LOCUS       DBNHXO010000001         3119 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1984552, whole genome shotgun sequence.
ACCESSION   DBNHXO010000001 DBNHXO010000000
VERSION     DBNHXO010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1984552"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(393..1163)
                     /locus_tag="AC162A_00005"
                     /pseudo
     CDS             complement(393..1163)
                     /locus_tag="AC162A_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(1205..2038)
                     /locus_tag="AC162A_00010"
     CDS             complement(1205..2038)
                     /locus_tag="AC162A_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR5009253.1"
                     /translation="MATPNVTYDDLSAGSPEEIVKAVAASRAKARFRFRTLFVLTWLI
                     LVGGLVLAILSTGRIDGGFLQEWGPYILLGVPLTIIVSVFSIFFATIFAVIGALGRLS
                     RTAPIYAVATLYVSLVRGTPLIVQVFFIYSALPEFGIVLPAFVAGVFALAFNYGAYLT
                     EIFRAGIQAVPRGQVEAAAALGMTERSTMRRIVLPQAIRIVIPAIGNDFISMTKDSAL
                     VSFVALQETFWRARTVGSANFQSFATLMLAALIYWVMTIVFSLFQERLEKRMAESEVR
                     Q"
     gene            complement(2118..2999)
                     /locus_tag="AC162A_00015"
     CDS             complement(2118..2999)
                     /locus_tag="AC162A_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR5009254.1"
                     /translation="MLAIVAIVAAACSSGGGGAATAAPGGSTTACADAGVTPAAGGLL
                     EKIKKANKLVVSTDPKYPPQSELKPDGTYQGFDIDVANEIAKRLCVGIEYTTPDWTAI
                     TAGGWGGRWDVSVGSMTITVDRLKALDFSPPYYYTPAQMAASTNSGITTLEGLAGKKI
                     CSGEGTTYDQWLNGTLDYGTGEDLGKPPAGITTTTLPTDTDCADQWKAGRFDFDGWLT
                     SQTVVDGAIAAGLPLVKVGDPVYYEPLAVAADKGGPATADFSPILKKIVDDMHADGFL
                     STASQKWFGADFTKAKS"
BASE COUNT          468 a         1032 c         1106 g          513 t
ORIGIN      
        1 gcttggcgta ctggtcgagg atgtggttga gctgtccctc gaggcgcgcg agctcgtccc
       61 cggtcagacc gaggcgtgcg aggtaggcga cgtgctcgac gtcggcacgg gacagggagg
      121 ccatgcccgc gatgatacgc ggcggagccc ccctgctagg gcttgtcgat ctcgggcaga
      181 tccggcatcc cctggctctc gtggggaccc tgcgggtggg cctcgtcgtg gaggtgctcg
      241 gggaccagct cgtcgtggag gtgcgccggg ccctggtcgt agggcacgtg cggctcgtcg
      301 agcccgtggg ctccaccctc ctcgtggatg tggacggcgc cggcgccctc gggctcgtga
      361 atcgtgccgc cctcgtggac gtgggcctcc cccagggtgc gggcgaggaa ctcccgcgtc
      421 cgcgggtcct gcgggttgtt gatgacctgc tcggggggcc cgacctcgac gaacttgccg
      481 ccgtcgatga agtagacgcg gtccgccgcc tcgcgggcga acgccatctc gtgggtcacg
      541 acgatcatcg tcgcgccttc gtgggcgagg tcctccatca cgttgatgac ctcgccgacg
      601 agcgtcgggt cgagggcgga ggtcggctcg tcgaacagca ggaccttggg ctccatcgtc
      661 agcgcccgag cgatcgcgac gcgctgcttc tgcccgccgg acagtcgccc gggatactcg
      721 tcccgcttct ccgacaaccc cactttggcc aggtacttct ccgcggtcgc gatcgcctcg
      781 tccttcggga cgcccttcac cctgaccggc gcctcgatca ggttgtcgat gaccttgagg
      841 tgcgggaaca ggttgaactc ctggaacacc atctgcgccc gcagccgaat ctgtcggatc
      901 tgctcccgat gctgcttgct ccgggaatgc agggggtcgg cgtggaccgt gaacccgtcg
      961 acctcgaccg agccgacggt cggctcctcg aggaagttga tgcaccgcag cagcgtgctc
     1021 ttgccagagc ccgacttgcc gatcaggcag atcgtctcct tggggtagac ctcgagggtg
     1081 acgccgcgca ggacgtgatt ggcgttgtag aacttctgga tgtcgagcgc gcggacgacg
     1141 ggtggcgcgc cggcgggcgt cagcgcgccc gggcgtgcga actggtcgcg gatctcggtc
     1201 gcggtcactg gcgcacctcg ctctcggcca tccgcttctc gagccgctcc tggaacagcg
     1261 agaacacgat ggtcatgacc cagtagatga gggcggcaag catgagcgtc gcgaagctct
     1321 ggaagttggc gctgcccacg gtccgcgccc gccagaacgt ctcctgcagg gccacgaacg
     1381 agaccagcgc ggagtccttg gtcatcgaga tgaagtcgtt gccgattgcc gggatcacga
     1441 tccggatcgc ctgcgggagc acgatccggc gcatcgtgga gcgctcggtc atgcccagcg
     1501 ccgcagcggc ctcgacctgc ccccggggca ccgcctggat ccccgcccgg aagatctccg
     1561 tcaggtacgc cccgtagttg aacgccaggg cgaacacgcc ggcgacaaac gcaggcagga
     1621 cgatgccgaa ctccggcaag gccgagtaga tgaagaacac ctggacgatg agcggtgtgc
     1681 cccgcacgag ggacacgtag agcgtcgcga cggcgtagat cggggccgtc cgcgacaggc
     1741 ggccgagcgc gccgatgacc gcgaagatcg tcgcgaagaa gatcgagaag acggacacga
     1801 tgatcgtgag cgggacaccc aacaggatgt acggccccca ctcctggagg aacccgccat
     1861 cgatccggcc cgtggacagg atcgcgagga cgaggccccc gacgaggatc agccacgtca
     1921 ggacgaacag cgtccggaac cggaaacggg ccttggcccg cgacgcggcg accgccttga
     1981 cgatctcctc cgggctcccg gcgctcaggt cgtcgtacgt gacgttcggc gtggccatcg
     2041 ctcctggtcc tcctcggaac gcggcgggga cgtccggtgg acgtccccgc gaggcgacta
     2101 ctggccggtt ggatgcgcta ggacttcgcc ttcgtgaagt cggcgccgaa ccacttctgg
     2161 gaggccgtgc tcaggaagcc gtcggcgtgc atgtcgtcga cgatcttctt caggatgggg
     2221 gagaagtctg cggttgccgg gccgcccttg tcggcggcca cggcgagcgg ctcgtaatag
     2281 acgggatcgc ccaccttcac gagcggcagc ccggccgcga tcgcgccgtc gacgacggtc
     2341 tgcgacgtca gccagccgtc gaagtcgaac cggccggcct tccactggtc ggcgcagtcg
     2401 gtatcggtcg gcagcgtggt ggtcgtgatg cccgccggcg gcttgccgag gtcctcgccg
     2461 gtgccatagt ccagcgtgcc gttgagccac tggtcgtagg tcgtgccttc gcccgaacag
     2521 atcttcttgc cggcgaggcc ctcgagggtc gtgatgccgg agttggtgct ggccgccatc
     2581 tgggccggcg tgtagtagta cggcgggctg aaatcgagcg ccttcaggcg gtcgacggtg
     2641 atggtcatcg agccgacgct gacgtcccag cggccgcccc agccaccggc ggtgatcgcc
     2701 gtccagtcgg gggtggtgta ctcgatcccg acgcacaggc gcttggcgat ctcgttggcg
     2761 acgtcgatgt cgaagccctg gtacgtgccg tcgggcttga gctccgactg cggcgggtac
     2821 ttcgggtcgg tcgagacgac gagcttgttg gccttcttga tcttctccag caggccaccg
     2881 gcagccgggg ttacgccggc gtcggcacac gccgtcgtgc tgccgccggg tgcggccgtg
     2941 gcggccccgc caccgccgga tgaacaggcc gccgcgacga tcgcgacgat ggcgagcatg
     3001 gacagtcgtc cacgtaggga catgtgctcc tcctaactcc agggcaccgc gagatgcgct
     3061 gcggcaccgt gagtgggcgg cattatgcgc ccgccggcgg ttcgatcgcc agcagcccg
//
LOCUS       DBNHXO010000002         7509 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_992426, whole genome shotgun sequence.
ACCESSION   DBNHXO010000002 DBNHXO010000000
VERSION     DBNHXO010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7509)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7509
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_992426"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="AC162A_00020"
     CDS             complement(<1..857)
                     /locus_tag="AC162A_00020"
                     /inference="COORDINATES: protein motif:HMM:NF025616.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HIR5009255.1"
                     /translation="MQASNRPTVALRVVVVLAVAFVAAACSGSSSQILSTVGRPIEST
                     TQQPVASPAQNGGNGPASGQNPGGNANAPSAPRDDLKIVYTGSLQLVVDDLAASVAKA
                     KAAVLATGGYIGGSNESNTGDRSVATVTYRIPATRWDDAIGTLRGFATKVVAEQTEAT
                     EVGGQIVDLEARLRNLRASEASLQAIAQATGSVSDLLEVEAQLTSVRGQIEQLDGQRA
                     QLEDQVAYGTLVTTFGLEVVQVKEAAAGWDPADDVDGALATMVGAGQTIVSGAIWFGI
                     VWLPLILVLV"
     gene            1004..2098
                     /locus_tag="AC162A_00025"
     CDS             1004..2098
                     /locus_tag="AC162A_00025"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007025965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HIR5009256.1"
                     /translation="MRLPFEPPLEPMLAKASDALPEGEGWLYEPKWDGFRALVFRDGD
                     EVYIQSRDLKPLDRYFPELGEPLRAQLPERCVLDGEVVIAHDGGLQFESLLLRIHPAE
                     SRVRMLAAETPASYVAWDLLALGDEDLRAVPQGDRRARLEGVLGGVTPPIHLTPATLD
                     RALAADWFDRFEGAGLDGVVAKRLDGAYQPGKRAMLKIKHERTADCVVAGFRWHKNGP
                     GTHVGSLLLGLFDDGGTLHHVGVTSTFTWERRAALAEELAPLREHALDDHPWGEWAEW
                     ASAGAADASGQRLPGATSRWNRGKDLSWEPLRTERVVEVAYDHLQGDRFRHGTTFRRW
                     RPDKRPADCRYDQLETTAPFELSRIFGPGA"
     gene            2107..3426
                     /locus_tag="AC162A_00030"
     CDS             2107..3426
                     /locus_tag="AC162A_00030"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR5009257.1"
                     /translation="MDNLSIPAGLVGTLAIVAFGVTILAVFATPVLRRMDRLAELRGP
                     LAIVPYALLLVAAGLAATASALGTVTAQHVGVREIATAALAILCITPVVGVLLVRLRY
                     GSTGDRTFYQQIAANRVSSALLVAVLFEVVAVTGFLIGAGIGLGFGVALIGGLVTAGI
                     AVTISVGATAFALFHGDSLILDVSGAKPATSRHEAQLLNVVAELSAAAAIPVPKVYVI
                     DTSAVNAMSIGRDPAHASLAVTRGALEGLDREELQGVVAHELGHIANLDSRHALLVAL
                     LVGAVVVMTALFLQLMIEIARNPWVGGDSISEAVGGLAAWLVVTVIGLLFAGTLRLFA
                     PFAALAVQAAVSRDREYLADATAVGITRNPAGLIAALGKLDHANAVIAEANGGTQHLW
                     IVNPVRESREGGRGWFATHPATSDRIARLRALAGRVTQPDLAAAAPD"
     gene            3507..4475
                     /locus_tag="AC162A_00035"
     CDS             3507..4475
                     /locus_tag="AC162A_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019326220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5009258.1"
                     /translation="MTPSTHPSSIARSGPTAVAFDGVSRRFGDTVALDEIDLGIAIGE
                     TVALLGPNGAGKTTAIGIMLGLLEPSTGLARTLGLAPHDAVRSGRVGAMLQSAGLPPG
                     ARVGELVDLARGLYPAPAGRDALLERAGLADLVERRAETLSGGEAQRVRFAFAIAGDP
                     DLVFLDEPTVAMDVESRRAFWDDMRRFAAEGRTVLFATHYLDEADQVADRIVVLDRGR
                     IVGDGSPAALKATVTERTVRFALPDGGGSDDPVLRSLPGVIGLDRHGQTASLLSRDAD
                     ETVRGLVAAGIAFRHLEVAGADLDAAFLALTSGPREPVGSSTPAAA"
     gene            4487..5278
                     /locus_tag="AC162A_00040"
     CDS             4487..5278
                     /locus_tag="AC162A_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009259.1"
                     /translation="MLATRSATRALPSVGPFLAYLRLEVRRSLRNRRYLVFTIVFPVM
                     LYVLYTAILPATASGPVAGLPWDVYFLVSMAAYGAIGAAMSQAAPIATERRAGWARQL
                     RVTPLPGIAYVAAKAASAMLLTVPALALVGLAGQVVNHVDIGVGTWVAVIVALAIGSL
                     PFAALGLLLGYLLDAESAQGGMVLSFFSLAILGGLFAPLEAFPAGLATIGRVLPSYHF
                     ANLGRSVVAGQLPDPTDIAVIAAWAVGLGVAAAWRYAADERSGRS"
     gene            5281..6483
                     /locus_tag="AC162A_00045"
     CDS             5281..6483
                     /locus_tag="AC162A_00045"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019350.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5009260.1"
                     /translation="MASPAAPAADGPQLTVSGPGGGRRRLRVPFVAATLFFTVFPFLS
                     LLANPPQPAVLALLVAGWAIFAAVLVALFRGGPFASPFGGWPMVVAVVAMIAIAAVTQ
                     VVFRTAGGSALYFYAGVTAARLTDERLALGGIAAAGLVATLAIGIASADWSDGLVVGV
                     TVATISLTLFSLARLGRANRELQAARRELADMAVAEERTRIARDLHDTLGHSLSLIAL
                     KSELARRVLPADPARAAAEIGDVERVARESLASVRETVSGYRQPTLAIELAGARTALR
                     AAGIEGEVEPAPDGLSRDVDAVLGWAVREGVTNVLRHSEARSARIRVVADGATRAVFV
                     EDDGHGGEATEGDHAPGWTGTGLTGLRERAAALGGTVEAGPVAGRGWRLHVAVPADRD
                     ATAGSAAT"
     gene            6480..7103
                     /locus_tag="AC162A_00050"
     CDS             6480..7103
                     /locus_tag="AC162A_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5009261.1"
                     /translation="MIRVLLAEDQAMVRGALAALLALEADLEVVGEVGRGDQVVPRAL
                     EARPDVALVDIEMPALDGLSAAAQLRAAVPACRVIILTTFGRPGYLRRAMEAGASGFL
                     LKDAPATELADAIRRVARGQRVVDSTLALAALSEGANPLSEREREALALSAASGTVAD
                     IAATLGLSEGTVRNHLSAAIQKVGARNRAEAARIASGKGWLAPETDG"
     gene            complement(7100..>7509)
                     /locus_tag="AC162A_00055"
     CDS             complement(7100..>7509)
                     /locus_tag="AC162A_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018326122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5009262.1"
                     /translation="VRLAGVSAGDDLAAIERERLRALVEIDLDRAAELHADDFQLVTP
                     SGRVLSKAEYLEAVRTRAIAYALWEAGEIEVRRHDDAAMIRYRSEMAFTVDGARTPRR
                     RFWHTDLYERRDGRWQVVWSQATEIRGPERPEA"
BASE COUNT          910 a         2768 c         2716 g         1115 t
ORIGIN      
        1 accagcacca ggatcagggg cagccacacg atcccgaacc agatggcacc gctgacgatc
       61 gtctggcccg cgccgaccat cgtcgccagc gcaccgtcga cgtcgtcagc gggatcccag
      121 cccgcggcgg cctccttgac ctggacgacc tccagtccga acgtcgtgac cagcgtcccg
      181 taggcgacct ggtcctccag ctgcgcccgc tggccatcga gctgctcgat ctggccgcgg
      241 acgctggtca gctgcgcctc gacctcgagc aggtcgctca ccgagcccgt ggcctgggcg
      301 atcgcctgga gcgacgcctc gctggcgcgc aggttgcgca ggcgggcctc gaggtcgacg
      361 atctggccgc ccacctcggt cgcctcggtc tgctcggcga cgaccttggt ggcgaacccg
      421 cggagagtgc cgatcgcgtc gtcccagcgc gtcgcaggga tgcggtaggt gacggtcgcg
      481 acggaacggt cgccggtgtt ggactcgttg gagccgccga tgtagccgcc ggtcgcgagg
      541 acggctgcct tggccttggc gaccgacgcc gccaggtcgt cgaccacgag ctgaagggag
      601 cccgtgtaca cgatcttgag gtcgtcgcgg ggcgccgacg gcgcgttcgc attcccgccc
      661 gggttctggc cggacgctgg gccgttgccg ccgttctgtg ccgggctggc aaccggttgc
      721 tgggtcgtcg actcgatcgg cctcccgacg gtcgagagga tctgggaact gctcccgctg
      781 cacgccgccg ccacgaaggc gacggccagg acgacgacca cgcggagcgc gacggtcggt
      841 cggttggacg cttgcatggc tggtcacctc gtgtcgggct cgttcgagcc catcgaccgc
      901 atcgggcgcg gcgacgtgag cgtggcccga ccccgcgctc acgtcgttcg gtgacaaccc
      961 gcaatcgcgc ctcccgggct tcccggcgcg ctatcgtgcc tgcatgcgac tccccttcga
     1021 accgccgctc gagccgatgc tcgcgaaggc gagcgacgcg ctgcccgagg gcgagggctg
     1081 gctctacgag ccgaagtggg acggtttccg cgcgctggtc ttccgggacg gcgacgaggt
     1141 ctacatccag agccgcgacc tcaagccgct cgaccgctac ttccccgagc tgggcgaacc
     1201 gctcagggca cagctcccgg agcgttgcgt gctcgacggg gaggtggtga tcgcccacga
     1261 cggcggactc cagttcgagt cgttgctgct gcgcatccac cccgccgagt cgagggtgcg
     1321 gatgctggcg gcggagacgc cggcgtccta cgtcgcgtgg gacctgctcg cgctgggtga
     1381 cgaggacctc cgcgccgtgc cccagggcga ccgacgggcc cgcctcgagg gcgtgctggg
     1441 cggcgtgacg ccgccgatcc acctcacccc cgcgacgctc gatcgcgcgc tggccgccga
     1501 ctggttcgac cggttcgagg gggccggcct cgacggggtc gtcgcgaagc ggctcgacgg
     1561 ggcctaccag cccggcaagc gggcgatgct caagatcaag cacgagcgca cggccgactg
     1621 cgtcgtcgcg ggctttcgct ggcacaagaa cggcccggga acgcacgtcg ggtcgctgct
     1681 gctcggcctg ttcgacgacg gcggcacgct gcaccacgtc ggcgtcacgt cgacgttcac
     1741 gtgggagcgg cgcgcagcgc tcgcggagga gctggcaccg ctgcgcgagc atgcgctcga
     1801 cgaccatcca tggggcgaat gggcggagtg ggcgtcggcg ggagcggccg atgcctcggg
     1861 ccagcggctg cccggagcca cctcacgctg gaaccggggc aaggacctgt cgtgggagcc
     1921 actgcgcacc gagcgggtcg tggaggtcgc ctacgaccac ctccagggcg accggttccg
     1981 ccacgggacg acgttccgcc gctggcgccc ggacaagcgg cccgcggact gccgctacga
     2041 ccagctggag accaccgcgc cgttcgagct gtcacgcatc ttcggccccg gcgcctagcc
     2101 cccacggtgg acaacctgag catcccggcg gggctcgtcg gcaccctggc gatcgtcgcg
     2161 ttcggggtga ccatcctcgc cgtgttcgcg acgccggtcc tgcggcggat ggaccgcctc
     2221 gcggagctgc gcggcccgct cgcgatcgtc ccgtacgcgc tgctcctggt cgccgccggc
     2281 ctggccgcca ccgccagtgc cctcggcacc gtgaccgccc agcacgtcgg cgtgcgggag
     2341 atcgcgaccg cggcgctcgc catcctgtgc atcacgccgg tcgtcggcgt cctgttggtc
     2401 cggctgcgct acggatcgac gggcgatcgg acgttctacc agcagatcgc ggccaaccgg
     2461 gtcagctcgg cgctgctcgt cgccgtgctg ttcgaggtcg tggcggtcac cgggttcctc
     2521 atcggcgccg gcatcggcct ggggtttggc gtcgccctga tcggtggcct ggtgaccgcc
     2581 gggatcgcgg tgacgatctc cgtcggcgcg acggcgttcg cgttgttcca tggcgactcg
     2641 ctgatcctcg acgtgtcggg ggccaagccc gccacgagcc ggcacgaggc gcagctgctc
     2701 aacgtcgtcg ccgagctgtc cgcggcggcg gccatccccg tccccaaggt ctatgtcatc
     2761 gacaccagcg cggtgaacgc gatgtccatc gggcgagacc ccgcgcacgc gtcgctcgcc
     2821 gtgacccgcg gcgcgctcga gggcctcgat cgggaggagt tgcagggcgt cgtcgcccac
     2881 gagctcgggc acatcgccaa cctcgactcg cgccatgcac tgctggtcgc gctgctggtc
     2941 ggggccgtcg tcgtgatgac cgcactgttc ctccagctga tgatcgagat cgcgaggaat
     3001 ccgtgggtcg gcggcgactc gatctcggag gccgtcgggg gcctcgcggc gtggctggtg
     3061 gtgacggtga tcgggctgtt gttcgccggc acgctgcgcc tgttcgcgcc gttcgccgcg
     3121 ctcgcggtcc aggcggcggt cagccgcgac cgcgagtacc tcgccgacgc caccgccgtc
     3181 gggatcaccc gtaatccggc cggcctgatc gccgccctgg gcaagctcga ccacgccaac
     3241 gccgtcatcg ccgaggcgaa cggcggtacg cagcacctgt ggatcgtcaa cccggttcgc
     3301 gagagccgcg agggcggccg cggctggttc gccacccacc cggcgacgag cgaccggatc
     3361 gcccgtctgc gcgcactggc cggccgggtg acgcagccgg acctggcggc cgctgcgccc
     3421 gactgacgca cgccgtgacg cgcgtcatgg cgcgttcacg acgccagtga cttcgcgggg
     3481 tcgcccgcca ggaagatcct cggggcatga caccaagcac gcacccttcc tccatcgccc
     3541 gatccggccc gaccgccgtc gccttcgacg gcgtctcgcg caggttcgga gacaccgtcg
     3601 ccctcgacga gatcgatctc ggcatcgcaa tcggcgagac cgttgccctg ctcgggccca
     3661 acggcgcagg caagaccacc gcaatcggga tcatgctcgg cctgctcgag ccctcgaccg
     3721 gcctggcccg aacgctcggc ctcgcgccac atgacgcggt ccgctccggg cgtgtcggcg
     3781 ccatgctgca gtcggcgggg ctcccgcccg gcgcgcgtgt cggtgagctc gtcgacctcg
     3841 cccgtgggct ctacccggcg ccggcggggc gcgacgctct cctcgagcgc gcgggccttg
     3901 ccgatctcgt ggaacggcgc gccgagacgc tgtccggcgg ggaggcgcag cgcgtccgct
     3961 tcgccttcgc gatcgcgggc gaccccgacc tcgtgttcct cgatgagcct accgtcgcca
     4021 tggacgtcga aagccgccgg gcgttctggg acgatatgcg ccggttcgcc gccgagggcc
     4081 gcaccgtgct gttcgcgacc cactacctcg acgaggcgga ccaggtcgct gaccggatcg
     4141 tggtcctcga tcgcggccgg atcgtgggcg acggatcccc agcggcgctc aaggccaccg
     4201 tgacggagcg gacggtgcgc ttcgcgctgc cggacggcgg cgggtcggac gaccctgtcc
     4261 tccgctcact gccgggggtc atcggcctcg accgacacgg ccaaacggcg tccctgctga
     4321 gccgcgatgc cgacgagacg gtgcgcggcc tggtcgccgc cgggatcgcg ttccgtcacc
     4381 tggaggttgc gggcgccgac ctcgacgccg ccttccttgc actgacgtcc gggccgcgtg
     4441 agcccgtcgg atcgtcgacg cccgcggcag cctgaaggag tccgtgatgc tcgccacccg
     4501 atccgccacg cgcgccctcc cgtcggtcgg cccgttcctc gcctacctcc ggctcgaggt
     4561 caggcgttcg ctgcgcaacc ggcgctacct cgtgttcacg atcgtgttcc cggtcatgct
     4621 ttacgtcctg tacacggcga tcctgcccgc gacggcgtcg ggcccggtcg ccggactgcc
     4681 gtgggacgtc tacttcctgg tgtcgatggc cgcctacggc gcgatcggcg ccgcgatgag
     4741 ccaggctgcg ccgatcgcca cagagcgtcg cgcgggctgg gccagacagc tgcgggtgac
     4801 gccactgcca gggatcgcct acgtggcggc gaaggccgcc tcggcgatgc tcctcaccgt
     4861 tcccgcgctc gccctggtcg gcctcgccgg acaggtcgtc aaccacgtgg acatcggcgt
     4921 cgggacgtgg gttgccgtca tcgtggcgct cgcgatcgga tcgctcccgt tcgccgcgct
     4981 gggcttgctg ctcggctacc tgctcgacgc cgagagcgcc caggggggca tggtgctctc
     5041 gttcttctcg ctggcgatcc tcggcgggct gttcgcgccg ctcgaggcct ttccggcggg
     5101 cctcgctacg attggccgcg tgctcccctc ctaccacttc gcgaacctcg ggcgatccgt
     5161 cgtcgccggg cagctgccgg acccgaccga catcgcggtg atcgcggcct gggcggtcgg
     5221 tcttggcgtg gcggcggcgt ggcgctacgc cgccgacgag cgctcaggcc ggtcctgagc
     5281 gtggcttcac ctgccgcacc ggccgccgac ggcccgcagc tgaccgtttc ggggcccggc
     5341 ggcggccgcc ggcggctccg ggtgccgttc gtcgctgcaa cgctgttctt cacggtcttc
     5401 ccgttcctct cgctgctcgc gaacccgccc cagcccgcgg tcctcgccct gctggtggcc
     5461 ggctgggcga tcttcgccgc cgtcctcgtc gcgctgttcc gcggcgggcc gttcgcaagc
     5521 ccgttcggcg gctggccgat ggtcgtcgcg gtggtggcga tgatcgcgat cgcggcggtg
     5581 acgcaggtcg tgttcaggac ggccgggggg tccgcgctct acttctacgc gggcgtgacg
     5641 gccgcgcggc tgaccgacga gcggctggcc ctcggcggga tcgcggccgc cggcctggtg
     5701 gccaccctcg cgatcgggat cgcctcggcc gactggagcg acgggctcgt cgtgggcgtc
     5761 accgtcgcca cgatctcgct caccctgttc tcgctggcca ggctgggacg cgcgaaccgc
     5821 gagctccagg cggcacgccg cgagctcgcc gacatggcgg tggccgagga gcgcacccga
     5881 atcgcccgcg atctccacga cacgctcggc catagcctgt cgctgatcgc cctcaagagc
     5941 gagctggcgc gtcgcgtcct tcccgccgac cctgctcggg ccgccgccga gatcggcgac
     6001 gtggagcgcg tcgcgcgcga gtcgctggcg tccgtgcgtg agaccgtcag tggctatcgc
     6061 cagccgacgc tcgccatcga gctggcgggc gcgaggacgg ccctgcgggc ggccgggatc
     6121 gagggcgagg tcgagcccgc acccgacggc ctgtcccgcg acgtggacgc ggttctcggc
     6181 tgggcggttc gcgagggcgt cacgaacgtt ctgcgccaca gcgaggcgcg gtccgcgcgg
     6241 atccgtgtgg tcgcggacgg cgcaacgaga gcggtcttcg tcgaggacga cggacacggc
     6301 ggcgaggcga ccgagggcga tcacgcccca ggctggaccg ggacagggct gacggggctg
     6361 cgggaacggg cggcggccct cggcggcacg gtcgaggcgg gacccgttgc tggccgtggc
     6421 tggcggctgc acgtcgccgt gcccgccgat cgcgacgcca cggccgggag cgccgcgacg
     6481 tgatccgcgt gctgctcgcg gaggaccagg cgatggtgcg cggcgccctg gcggccctgc
     6541 tcgcgctcga ggcggacctc gaggtcgtcg gcgaggtcgg tcgtggcgac caggtcgttc
     6601 ccagggccct cgaggcgcgt ccggacgtcg cgctcgtcga catcgagatg ccggcgctcg
     6661 acggtctctc ggcggccgcc cagctgcggg ccgccgtgcc ggcgtgccgc gtgatcatcc
     6721 tcacgacgtt cgggcggccc ggatacctgc ggcgagcgat ggaggccggc gcatcggggt
     6781 tcctgctgaa ggacgcgccc gcaaccgagc tcgccgacgc gatccggcgc gtggcgcggg
     6841 gccagcgggt cgtcgactcg acgctcgccc tcgcggcgct cagcgagggt gcgaatccgt
     6901 tgtccgaacg ggaacgtgag gcgctcgcgc tgtcggcggc cagcggcacc gtcgccgaca
     6961 tcgccgcgac cctcggactg tccgagggga cggtccgcaa ccacctctcg gcggcgatcc
     7021 agaaggttgg cgcccggaac agggcggagg ccgctcggat cgcgtcgggc aagggctggc
     7081 tcgcgcccga gacggacggc taggcctccg gccgctccgg gcccctgatc tcggtcgcct
     7141 gcgaccagac cacctgccag cggccgtcgc gtcgctcgta gaggtcggta tgccagaacc
     7201 gccgccgcgg cgtccgcgcc ccgtcgacgg tgaacgccat ctcggagcga tagcggatca
     7261 tcgccgcatc gtcatggcgc cggacctcga tctccccggc ctcccacagc gcgtaggcga
     7321 tcgcgcgggt ccgcacggcc tcgaggtact cggccttcga gagcactcgc ccggacggcg
     7381 tgaccagctg gaagtcgtcg gcgtgcagct cggccgcccg gtcgaggtcg atctccacca
     7441 gcgcccggag ccgctcccgc tcgatcgccg cgagatcgtc gccggcgctc actccggcga
     7501 gccggacga
//
LOCUS       DBNHXO010000003         2467 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4762939, whole genome shotgun sequence.
ACCESSION   DBNHXO010000003 DBNHXO010000000
VERSION     DBNHXO010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4762939"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="AC162A_00060"
     CDS             complement(<1..1421)
                     /locus_tag="AC162A_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP and VCBS repeat-containing protein"
                     /protein_id="HIR5009263.1"
                     /translation="MKTPTLTSRKFPLSLLSFALALLSWPAPVAAASTAAMPSDFNGD
                     GYADMVVGSIYEDHEAGGITVLYGSPRGLTSDGSQRFSQDTPGIEGKPRRDRGYDGDG
                     SHFGAVLASGDFNRDGRADLAVGVPSDTVGDQDYAGSVTVLYGSKHGLTARDSQRLTE
                     ANVAGTPQFGDFFGASLAAGDFDGDGFPDLAVGVPGEFDPPGSGIAQVLHGGSDGLNP
                     SRTYALTMDMTGSADPNLTFPASLAAGDLDGDGIDDLAAGAPDDSCDRTACVTEGGDV
                     AVYYGSGGVGLSGDGSQRWSQDSEGVLDASESNDMFGASLETGDFDGDGYGDLAIGVP
                     GERVADALYDYGEGAVAVLYGTPTGLSADGNQRWDEDSPGVPGTPEHVGLFGAALAAG
                     DFDGDGADDLAIGAPFHDVGSAFGAGIVVVLRGSAGEGLTSTGAQRLTQASAGVPSKP
                     EGYDEFGTALAAADYGRSGRDDLA"
     gene            complement(1482..2252)
                     /locus_tag="AC162A_00065"
     CDS             complement(1482..2252)
                     /locus_tag="AC162A_00065"
                     /EC_number="2.8.3.-"
                     /EC_number="4.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004595063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HIR5009264.1"
                     /translation="MAFSKSEMMIVAAARELAGQHVCFVGVGLPNIAVNLAKRTVAPD
                     LELVYEAGVFGARPARLPLSIGDPTIVTGATAVVSMFELFAFYLQRGLIDVGFLGAAQ
                     IDRFGNLNSTVIGDYAAPKTRLPGSGGACEIAINARNVFVIMRQSARSFVEKIDFRTS
                     PGNLGGAEQAEAIRRAGGWNGRGPSVVVTDLGIWHFDETGEMRLDWLHPGVTLDDIKA
                     TIAWEPRISDALAVTPEPTTEELRLVREELDPGGVYTK"
     gene            complement(2252..>2467)
                     /locus_tag="AC162A_00070"
     CDS             complement(2252..>2467)
                     /locus_tag="AC162A_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR5009265.1"
                     /translation="YVQGAYDRDNRFYLDWDPISRDEDLVQAWLREWVYDLDGRASYV
                     EKLGAERVASLKPGPAPSGSVDYGSYG"
BASE COUNT          358 a          893 c          849 g          367 t
ORIGIN      
        1 gcgaggtcgt cgcggcccga gcggccatag tcggctgccg ccagcgcggt gccgaactcg
       61 tcgtagccct cgggcttgct cgggacgccg gcgctcgcct gggtcaagcg ctgggcgccg
      121 gtcgacgtca ggccctcgcc ggccgaccca cgaaggacga cgacgatacc cgcgccaaac
      181 gccgagccga cgtcgtggaa cggagccccg atcgcgaggt catctgcccc gtctccgtcg
      241 aagtcgccgg ccgcaagcgc ggccccgaat aggcctacgt gctcgggtgt ccccgggacg
      301 cccgggctgt cctcgtccca gcgctggttg ccgtcggcgg acagtccggt cggggtcccg
      361 tacagcaccg cgacggcccc ctcgccgtag tcgtagaggg cgtcagccac gcgctcgccc
      421 ggcaccccga tcgctaggtc gccatacccg tcgccgtcga agtcgccggt ctcgagcgat
      481 gccccgaaca tgtcgttgct ctcgctcgcg tccaggacgc cctcgctgtc ctggctccat
      541 cgctggctgc cgtcgcccga gagcccgaca ccacccgagc cgtagtacac ggccacgtcg
      601 ccgccctcgg tcacgcaggc tgttcggtcg caactgtcgt cgggcgcgcc ggcagcgaga
      661 tcgtcgatcc cgtccccgtc gaggtcgccg gccgcgagcg acgccggaaa tgtcaggttc
      721 ggatctgctg agccggtcat gtccatcgtc agggcatacg ttctgctcgg gttcagtcca
      781 tcggacccac cgtggagcac ctgggcaatc ccgcttcccg gggggtcaaa ctcccctgga
      841 acgcccacgg cgaggtccgg gaacccgtcg ccgtcgaagt cgcccgcggc cagcgacgct
      901 ccgaagaagt cgccgaactg cggcgttccg gccacattcg cttccgtgag ccgctggctg
      961 tcgcgggccg tgagcccatg cttcgacccg tacaggacgg tgaccgatcc cgcgtagtcc
     1021 tggtcgccaa ccgtgtcgga ggggacgcca acggcgaggt ccgcgcgccc gtcgcggttg
     1081 aagtcgcctg acgcgaggac ggcgccgaag tgactcccat cgccgtcgta gcctcggtca
     1141 cgtcgcggct tgccctcgat gcccggcgtg tcctgtgaga agcgctggct gccatccgag
     1201 gtcaggccac gcggcgagcc gtacaggacg gtgataccgc cggcctcgtg gtcctcgtag
     1261 atggacccca cgaccatgtc ggcgtagccg tcgccgttga agtcggacgg catcgcggcc
     1321 gtcgaagccg ctgcgacggg cgcgggccag gacaggaggg ccagcgcgaa cgagagcagc
     1381 gagagcggga acttgcgcga cgtcaacgtc ggtgtcttca cgtcggccct agaacgtccg
     1441 acggtccagg ttgtgacagc cgccgatcgg ccaccggccg cctacttcgt gtagacgccc
     1501 ccgggatcga gctcttcgcg gaccaggcgc agctcctccg tcgttggctc gggggtcacg
     1561 gcgagcgcgt cgctgatgcg cggctcccag gcgatcgtcg ccttgatgtc gtccagcgtc
     1621 acgccggggt ggagccagtc caggcgcatc tcgccggtct cgtcgaagtg ccagatcccg
     1681 aggtccgtga cgaccaccga cggtccccgg ccgttccagc cgccggcccg ccggatcgcc
     1741 tcagcctgct cggcgccgcc gaggttgccg ggcgacgtcc ggaagtcgat cttctcgacg
     1801 aacgagcgcg cggactggcg catgatcacg aacacgttgc gcgcgttgat cgcgatctcg
     1861 cacgcgccgc cggagccggg cagccgtgtc ttcggcgccg cgtagtcgcc gatcacggtg
     1921 gagttgaggt tgccgaaccg gtcgatctgc gccgccccca ggaacccgac gtcgatgagg
     1981 ccgcgctgga ggtagaacgc gaacagctcg aacatcgaga ccaccgcggt cgccccggtg
     2041 acgatggtcg ggtcgccgat ggacagcggg agccgcgccg gccgggcccc gaacacgccc
     2101 gcctcgtaga cgagctccag gtccggggcg accgtccgct tcgcgaggtt gacggcgatg
     2161 ttcggcagcc cgacgcccac gaagcagacg tgctggcccg ccagctcgcg cgcggcggcg
     2221 acgatcatca tctcggactt cgagaacgcc atcagccgta gctcccgtag tcgaccgagc
     2281 cggatggggc cggacctggc ttgaggctcg cgacgcgctc ggcgccgagc ttctcgacat
     2341 aggacgcgcg gccgtcgagg tcgtagaccc actcgcgcag ccaggcctgg accaggtcct
     2401 cgtcgcggct gatcgggtcc cagtcgaggt agaagcggtt gtcgcggtcg taggcgccct
     2461 gcacgta
//
LOCUS       DBNHXO010000004         4009 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3969298, whole genome shotgun sequence.
ACCESSION   DBNHXO010000004 DBNHXO010000000
VERSION     DBNHXO010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3969298"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2054)
                     /gene="lon"
                     /locus_tag="AC162A_00075"
     CDS             complement(<1..2054)
                     /gene="lon"
                     /locus_tag="AC162A_00075"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR5009266.1"
                     /translation="MAKRPSTPEEKPAATPRELPLVALRETVIFPEMIVPLQVGREKS
                     VAALNAAVENGGPIALVTQRQPEQEEISDPSELYAIGTLAKIAQVVQLQDGTVRAIVQ
                     GQGRIRVRAFASTSPYLAAEIDELSDDVPTGVEIQALVRSVQAQIEQYVANGAPVPPE
                     AAVAARNITEPGLLADMVAYSPDMSTEQRQELLETIDVEQRLKLVSAFLARQIEILEL
                     KGKIQSEVKSEMDKTQREYILREQLKAIQRELGEDDPQQAEINELRDKVEAAGMPDEI
                     KARAIKEIDRMSRIPSASPEVGVIRTYVDWLVSLPWNVSTDDRLDIREAALILDEDHY
                     GLEKVKERILEYLSVRTLADTIRSPILLFVGPPGVGKTSLGKSIAKAMGRKFVRMSLG
                     GIHDEAEIRGHRRTYIGALPGRIIQSVKTAGTNNPVFMLDEVDKIGMDFRGDPSSALL
                     EVLDPEQNFSFQDNYLEVPFDLRRVLFVATANLLDPIPAALRDRMEVILLPGYTQHEK
                     VEIARRFLVPKQMENHGLTAKRVEITDEVLTELVQAYTKEAGVRNLERELASIMRKVA
                     RQVAEGRKRKTIVDVKKLEEYLGPPRFEYGELEAVDQTGSATGLVVTEVGGDVVAVEV
                     TLMEGKEEFILTGQLGDVMRESARAALSWIRSNAAELGIEREVFEKHTLHIHVPAGAI
                     PK"
     gene            complement(2119..2505)
                     /locus_tag="AC162A_00080"
     CDS             complement(2119..2505)
                     /locus_tag="AC162A_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR5009267.1"
                     /translation="MALRRPERDPTQPVYVISVAATLVSAHPRTLRIYEDEGLVCPAR
                     TPTNIRLYSEEDIRRILWIRHLTRDRGVNLAGVRTLFELEERLGTRILEALYDEGTRV
                     KNEEAAARAAARPTPAGSDRDPGKTA"
     gene            complement(2768..>4009)
                     /locus_tag="AC162A_00085"
     CDS             complement(2768..>4009)
                     /locus_tag="AC162A_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HIR5009268.1"
                     /translation="FTLTGDVTNFVVKATDITCGDCKAKYWVTRSIGSGATSADLINS
                     DTLPGSGGVWFSGFMLTADDYFLVISVTQGSATWGGTTGAAMTHNGNVDGSDYAASPM
                     DASYPPRSASFAVIAGEGKLAYEVTGSQPDQPVDPPPLPDADGDSVPDATDNCPNDAN
                     PGQADDDGDGIGDACDTPDVPPPAPDADGDDVPDATDNCPEVANPGQADDDNDGVGNA
                     CDTPDVPPPAPDADGDGVPDATDNCPNDANPGQADDDNDGVGDACDTPDAPPPPDDDL
                     VVPTLATGAKVAFRGRFLPDGAVVWAGTPVREWAKLGGLLPGARGKVTYWIRRQGDGV
                     PNCSTGAGARELGTRRVWHRGVLGSDIAWLRNAGRYELWATYSGDKRHAEASSPCGSQ
                     TIVVKEKPKRHGHHHGDRKRR"
BASE COUNT          501 a         1323 c         1466 g          719 t
ORIGIN      
        1 tccttgggga tcgccccggc ggggacgtgg atgtggagcg tgtgcttctc gaacacctcg
       61 cgctcgatgc cgagctcggc ggcgttcgac cggatccagg acagggcggc cctggccgac
      121 tcgcgcatga cgtcgcccag ctggccggtg aggatgaact cctccttgcc ctccatcagg
      181 gtgacctcga cggcgaccac gtcgcctccg acctcggtca cgaccaggcc cgtcgccgag
      241 cccgtctggt ccacggcctc gagctcgccg tattcgaagc gcggcggccc caggtactcc
      301 tcgagcttct tgacgtcgac gatcgtcttg cgcttgcggc cctcggcgac ctgtcgggcg
      361 accttgcgca tgatgctggc aagttcacgc tccaggttcc ggacgcccgc ctccttcgtg
      421 taggcctgga cgagctcggt gaggacctcg tccgtgatct ccacgcgctt ggcggtgagg
      481 ccgtgattct ccatctgctt ggggacgagg aaccgccgcg cgatctcgac cttttcatgc
      541 tgcgtgtagc ccggcaggag gatgacctcc atccggtcgc gcagggcggc cgggatcgga
      601 tcgagcaggt tcgccgtggc gacgaagagc acgcgccgga ggtcgaacgg gacctcgagg
      661 tagttgtcct ggaagctgaa gttctgctcc gggtccagga cctcgagcaa ggccgacgac
      721 gggtcgccac ggaagtccat gccgatcttg tcgacctcgt cgagcatgaa cacggggttg
      781 ttcgtgcccg cggtcttgac gctctggatg atccgaccgg gcaaggcgcc gatgtaggtg
      841 cggcgatggc cccggatctc cgcctcgtcg tggatgccgc cgaggctcat ccggacgaac
      901 ttgcggccca tcgccttggc gatgctcttg cccaggctgg tcttgcccac gccggggggg
      961 cccacgaaca gcaggatcgg gctgcggatc gtgtccgcga gcgtgcgcac ggagaggtac
     1021 tcgaggatgc gctccttgac cttctcgagg ccgtagtggt cctcgtcgag gatcagggca
     1081 gcctcgcgga tgtccagccg gtcgtcggtc gagacgttcc agggcagcga cacgagccag
     1141 tcgacgtagg tgcggatgac gccgacctcg ggggaggccg acgggatccg gctcatccgg
     1201 tcgatctcct tgatcgcccg ggccttgatc tcgtccggca tgccggccgc ctcgaccttg
     1261 tcgcgcagct cgttgatctc ggcctgctgc gggtcgtcct cgcccagctc gcgctggatg
     1321 gccttgagct gctcgcggag gatgtactcc cgctgggtct tgtccatctc ggacttgacc
     1381 tcgctctgga tcttcccctt gagctcgagg atctcgatct ggcgggcgag gaacgccgag
     1441 acgagcttca ggcgctgctc gacgtcgatg gtctcgagga gctcctggcg ctgctcggtc
     1501 gacatgtcgg ggctgtaggc caccatgtcg gcgaggaggc cgggctcggt gatgttgcgg
     1561 gcggcgacgg ccgcctcggg cggcaccggc gcgccgttgg ccacgtactg ctcgatctgg
     1621 gcctggacgc tgcgcacgag cgcctggatc tcgacgcctg tcgggacgtc atcggacagc
     1681 tcgtcgatct cggcggcgag gtagggggac gtgctcgcga aggcgcggac gcggatgcgg
     1741 ccctgaccct ggacgatggc ccgcacggtc ccgtcctgga gctgcacgac ctgggcgatc
     1801 ttggccagcg tgccgatggc gtagagctcg gagggatcgg agatctcctc ctgctcgggc
     1861 tgccgctggg tgacgagcgc gatcgggccg ccgttctcga cggcggcgtt gagcgccgcg
     1921 acgctcttct cgcgccccac ctggagcggg acgatcatct ccggaaagat gaccgtctcc
     1981 cggagcgcga cgagcggcag ctcacgcggg gtggctgcgg gcttctcctc cggggtcgac
     2041 ggtcgcttgg ccatcagggt ctccttgaat gccgggtttc ggtccggcgc gctggtgcgg
     2101 tgattcggtg ctgtgacgtc atgcggtctt gcccgggtcc cggtcgctgc cggcgggcgt
     2161 cggtcgggcg gcggcccgag cggccgcctc ctcgttcttc actctggtgc cttcgtcgta
     2221 cagggcctcg aggatccggg ttccgaggcg ctcttcgagc tcgaacagcg tccggacgcc
     2281 ggcgaggttc acgccgcggt cccgggtcag gtggcggatc cagaggatcc tccggatgtc
     2341 ctcctccgag tagaggcgga tattggttgg cgttcgtgcc ggacagacga ggccctcgtc
     2401 ctcgtagatg cggagcgtgc gggggtgggc ggagaccagc gtcgccgcga cgctgatcac
     2461 atagaccggt tgggtcgggt cccgttccgg tcggcggaga gccatcagtc agagtcctct
     2521 gccggcgtcc gagccggcgt cgtggtggtc gttgcgatgt ccgacgggac ggctcccgag
     2581 gacatgcatc agcctagaac cttgacaatg gcgttgtcaa gacacgtatc ggcagaacgg
     2641 tcgattgcct gtctgcgcca gcgtctcgca ggggacaaac gaacacggcg ccgcgggtga
     2701 ccccacggcg ccgtgccgtt gcgctgcgaa tccgagcggt gctcgggcgt ttcgttgatc
     2761 cgggccgcta gcgccgcttg cggtcgccgt gatggtgacc gtggcgcttc ggcttctcct
     2821 tgacgacgat cgtctggctg ccgcacgggc tcgacgcctc ggcgtggcgc ttgtcacccg
     2881 agtacgttgc ccagagctcg taccgtccgg cgttgcgcaa ccaggcgatg tccgatccga
     2941 ggacgccgcg gtgccacacg cgccgggtgc ccagctcgcg cgcgcccgcg ccggtcgagc
     3001 agttcggtac gccgtcgccc tgccggcgga tccagtaggt caccttgccg cgggcgcccg
     3061 gcagcaggcc gccgagcttg gcccattcgc ggaccggcgt gccggcccat acgaccgccc
     3121 catccgggag gaaccggccg cggaacgcca ccttggcgcc ggtcgcgagc gtcggcacga
     3181 cgaggtcgtc gtcgggcggg ggcggtgcgt ccggcgtgtc gcaggcgtcg cccacgccgt
     3241 cgttgtcgtc atcggcctgg ccggggttcg cgtcgttggg gcagttgtcg gtggcatccg
     3301 gcacgccgtc gccatccgcg tcgggcgcgg gcgggggcac gtcgggcgtg tcgcaggcgt
     3361 tgcccacgcc gtcgttgtcg tcatcggcct ggccggggtt cgcgacctcg gggcagttgt
     3421 cggtggcgtc cggcacgtcg tcgccatccg catcgggcgc gggcggcggc acgtcgggcg
     3481 tgtcgcaggc gtcgccaatg ccgtcgccgt cgtcatcggc ctggccgggg ttcgcgtcgt
     3541 tggggcagtt gtcggtcgcg tccggcacgc tgtcgccatc cgcgtcgggc aggggcggcg
     3601 gatcgacggg ctggtcaggc tgggagccgg tcacctcgta ggcgagcttg ccctcgccgg
     3661 cgatcaccgc gaaggacgcg ctccggggcg gatagctggc gtccatcggc gaggcggcgt
     3721 agtcggagcc gtcgacgttg ccgttgtgcg tcatcgcggc gccggtcgtt ccgccccagg
     3781 tcgcgctgcc ctgggtgacc gagatgacca ggaagtagtc gtccgcggtc agcatgaagc
     3841 cgctgaacca gacgcccccg gatccgggca gggtgtccga gttgatgagg tcggcggacg
     3901 tcgcgccgct cccgatcgag cgggttaccc agtacttggc cttgcagtcg ccgcacgtga
     3961 tgtccgtcgc cttgacgacg aagttggtga cgtcgcctgt gagcgtgaa
//
LOCUS       DBNHXO010000005         1940 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2977128, whole genome shotgun sequence.
ACCESSION   DBNHXO010000005 DBNHXO010000000
VERSION     DBNHXO010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2977128"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..>1940)
                     /locus_tag="AC162A_00090"
     CDS             complement(165..>1940)
                     /locus_tag="AC162A_00090"
                     /inference="COORDINATES: protein motif:HMM:NF019522.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HIR5009269.1"
                     /translation="GVDSGTVHAHPIPDDRPMPRRRVPRELIVVVLGALLVLAVLLVP
                     DLMPPPDETTPPVVALHGRIESVALPDPTSVDEGAPPVPTAKVLILEGARQGEVLDAQ
                     IEGPGGSQLIGDYKPGDEVVVSITRSADEAAPYVAVADRWRVVPLGLLVGLFAVAVVA
                     VGGWRGARALVALGLTIAIILKILLPLIIRGVPPLPLAVVTAAGITAITILLTEGRSR
                     ASLAAILGTSAALAIAGLLAAAATAAASFTYSAGSDLAFLVTLSGSGLDLRGVLLAAM
                     LLGAVGVLDDVTVTQAVVVEELAARSPLRGRALFMSAHAIGRSHIGATVNTLFLAYVG
                     AALPLLVVLLVSHPPLALVTNSEDIATEIVRTLAGSLGIVAAVPLTTFIATTLVGRDR
                     PTEAGEDPAAPQRRSSWPVVGVGVVVLLLLGATAILPATQPGRPPLATEPPFQAPPDD
                     GLGFLGTPPSDYVAPPTDDPDGHEPALAELGYPVPISIGTQTVGTAAVDEASVTGKAL
                     HKVKVVVSYAAIGTWPLDPGVWEILTSDGTEIPLTPTPAMSGTLEAGDDATFTLTGET
                     SKRLRDAFVAYRDSPETFAFLVALP"
BASE COUNT          302 a          674 c          732 g          232 t
ORIGIN      
        1 acatcgtccg ccgctacgtc gaagcccacg atcccggggc cgcgccggcc gactacttgc
       61 aggacggggg tgtcgtcgtg ctcatcgagg cgtcgtcgat tcgggcctgg gactacgcgg
      121 acgcggcata tgtctgacca acgaccacga ccgggtggcg ccagctacgg cagcgccacc
      181 aggaaggcga acgtctccgg cgagtcgcgg taggcgacga aggcgtcgcg caggcgcttc
      241 gacgtctctc ccgtcagcgt gaatgtcgcg tcgtcaccgg cctcgagggt gccgctcatc
      301 gcgggcgtcg gggtcagcgg gatctccgtc ccgtccgagg tcaggatctc ccagacgccc
      361 ggatcgagcg gccaggtccc aatcgcggcg tacgagacaa cgaccttgac cttgtgcaag
      421 gccttgccgg tgacggaagc ctcatcgacc gcggcggtcc cgacggtctg cgtcccgatc
      481 gagatcggca ccggatagcc gagctcggcc agcgccggtt catgcccgtc cgggtcgtcg
      541 gtcggcggcg cgacatagtc cgatggcggc gtgcccagga agccgaggcc gtcgtcaggc
      601 ggcgcctgaa agggcggctc ggtcgcgagc ggaggacgcc cgggctgggt cgccgggagg
      661 atcgccgtcg ccccgagcag gaggagcacg accacgccaa cgcccacgac cggccaggac
      721 gaacgacgct gcggagccgc cgggtcctcg ccggcctccg ttggccggtc gcggcccacg
      781 agcgtggtcg cgatgaacgt ggtcaacggc acggccgcga cgatcccgag gctgccggcc
      841 agcgtgcgca cgatctcggt cgcgatgtcc tcgctgttgg tgacgagggc gaggggtgga
      901 tggctcacca gcagcacgac cagcagcggc agcgcggcgc cgacataagc caagaacagc
      961 gtgttgacgg tcgccccgat gtgcgagcgg ccgatcgcat gcgcgctcat gaacagggcc
     1021 cgaccccgca gcgggctacg cgcggcgagc tcctcgacca cgaccgcctg cgtcaccgtc
     1081 acgtcgtcga gcacccctac cgcgcccagg agcatcgcgg ccagtagcac gccccggaga
     1141 tcgaggccgg aaccgctgag cgtgaccagg aacgcgaggt cggagccggc cgagtaggtg
     1201 aagctggcgg ccgcggtggc agcggcggcg aggaggccgg cgatcgcgag ggcggcggat
     1261 gtgccgagga tggcggcgag gcttgcgcgc gagcgcccct cggtcagcag gatcgtgatc
     1321 gcggtgatgc cggcggccgt cacgaccgcg agcggcaacg gcggcacgcc ccggatgatc
     1381 agcgggagca ggatcttgag gatgatcgcg atggtgagcc cgagggcgac gagcgcccgc
     1441 gcgccgcgcc agccgccgac ggcgacgacg gcgaccgcga acagcccgac gagcagcccg
     1501 agcgggacca cccgccagcg atcggcgacg gcgacgtagg gcgccgcctc gtcggcgctc
     1561 cgggtgatcg agaccaccac ctcgtcgccg ggcttgtagt cgccgatcag ctgcgagccg
     1621 cccggtccct cgatctgggc gtcgaggacc tccccctgac gcgcaccctc gaggatgagg
     1681 accttggcgg tcggcacggg cggcgctccc tcgtcgacgc tcgtcgggtc gggcagggcg
     1741 acggactcga tgcgaccgtg gagcgcgacc acgggcgggg tggtctcgtc cggcggcggc
     1801 atcaggtccg gcacgaggag gaccgccagg acgagcagcg cgccgaggac gaccacgatc
     1861 agctcgcgcg gcactcgccg gcgcggcatg gggcggtcgt cggggatcgg gtgagcgtgc
     1921 accgtccccg aatctacccc
//
LOCUS       DBNHXO010000006         1909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_688, whole genome shotgun sequence.
ACCESSION   DBNHXO010000006 DBNHXO010000000
VERSION     DBNHXO010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_688"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..841)
                     /locus_tag="AC162A_00095"
     CDS             complement(83..841)
                     /locus_tag="AC162A_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013252.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HIR5009270.1"
                     /translation="MVEQLNLRLEPALPRPGDVAPMWPTARPDPFDSPDYLFEPTWGG
                     HRVLVFVGPSERPGDGAVRIVDPAGRDLADRLPELTGLAVRVAARSAVLDGELVVVDA
                     RGRADDDALRERLNGKPGRPVACLVFDILHLDGRWLLNVPLEKRRATLRKVLRPGDEV
                     VVVPAITGEGRALHAAVTAQGIAGVLARRRSSPYLPGVHSALWRSIMAGPKPEDAADA
                     GAEPAVSAVADDAPATAPVLALFRRLPFEEDPAD"
     gene            963..>1909
                     /locus_tag="AC162A_00100"
     CDS             963..>1909
                     /locus_tag="AC162A_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5009271.1"
                     /translation="MPRPARPDDVYRLSVPFDPRLSPDGRFVAFTVKRAAVGRDGYRH
                     AVWLAPTDGSAPARQVTIGSRTDRHARFSPDGSTLAFISDRRLLVEEEPTRPKEAKDR
                     LDCDQLFLLPLDGGEARRLTDLPRGVNAFAWSPDGRSLAVLSSSLGATADEETKKRGR
                     PTPPKPGETPLSDYRYIDRLGYQYNGAGFIDDRDAHLWLVDATSGEARSLVSGPTPEG
                     EPAWSPDGTRIAFTANRRPNPDLDGRSSVFVVEVATGAVTTIAGGPDAAFFSPTWTAN
                     AASILALGDRFPRAGYRTGIWRFSADGSDAGRTGGLDLLA"
BASE COUNT          245 a          737 c          692 g          235 t
ORIGIN      
        1 tcgcgatccg cgtgtaccgg gccggcgtgc tgatgtacgg ccagccgccg acgatgcgtt
       61 cgttcgcgcg ggccgtccgg ggctagtcgg ccgggtcctc ctcgaacggg agacggcgga
      121 acagggcgag caccggcgcc gtggccggcg cgtcgtccgc gacggcggac acggcgggtt
      181 ccgcgcctgc gtcggccgca tcttcgggct tcggtccggc catgatcgag cgccacaggg
      241 cgctgtgcac gcccggcagg tacgggcttg aacgccgacg ggcgagcacg cccgcgatcc
      301 cctgggccgt cacggcggca tggagcgctc gcccctcgcc cgtgatcgcg ggtaccacga
      361 ccacctcgtc gccgggccgc aggaccttgc gcagcgtcgc gcgccgcttc tcgagcggga
      421 cgttgagcag ccagcgcccg tcgaggtgca ggatgtcgaa cacgaggcag gcgaccggcc
      481 ggccgggctt cccgttgagt cgctcgcgca gcgcgtcgtc gtcggcccgc ccgcgagcgt
      541 cgaccacgac cagctccccg tccagcaccg cggatcgtgc ggcgacgcgc acggcgagcc
      601 ccgtcagctc gggcaggcgg tcggcgaggt cgcgacccgc tggatcgacg atccgcacgg
      661 cgccgtcgcc cggccgctcg gagggaccaa cgaacacgag cacccggtgg ccaccccacg
      721 tcggctcgaa caggtagtcg ggcgagtcga aggggtccgg gcgcgcggtc ggccacatcg
      781 gcgccacatc gcccgggcga ggcagcgccg gctcgagccg gaggttcagc tgctcgacca
      841 tcgcccgccg atggtagccc gcccacgggc gatagactca cggccaacga ccatcggccg
      901 cccgctgccc gcggcggccc agcccgcggc ggctgtcgcc ggccacgacc ccggaggtga
      961 acgtgccccg tcccgcacgc ccggacgacg tctatcggct gtccgtcccg ttcgatcccc
     1021 ggctgtcgcc ggacggccgg ttcgtcgcgt tcaccgtgaa gcgggccgcc gtcgggcgcg
     1081 acggctaccg gcatgcggtg tggctcgcgc cgaccgatgg ctcggcgccg gcccggcagg
     1141 tcacgatcgg cagccggacg gaccggcacg cgcggttctc gcccgacggc agcaccctcg
     1201 cgttcatctc cgaccggcgg ctgctggtcg aggaggaacc gacccggccg aaggaggcga
     1261 aggaccgcct cgactgcgac cagctgttcc tgctgccgct cgacggcggc gaggcgcggc
     1321 ggctcaccga cctgccgcgg ggcgtgaacg ccttcgcgtg gtcgcccgac gggcgctccc
     1381 tggcggtgct gtcgagctcg ctcggcgcca ccgcggacga ggagacgaag aagcgcggcc
     1441 gccccacgcc gccgaagccc ggcgagaccc cgctgtcgga ctaccgctat atcgaccgcc
     1501 tgggctacca gtacaacggc gccggcttca tcgatgatcg cgacgcccac ctgtggctgg
     1561 tcgacgcgac gagcggcgag gcccgctcgc tggtctccgg tccgacgccc gagggtgagc
     1621 ccgcctggag ccccgacggc acccggatcg cgttcacggc caaccgcagg ccgaacccgg
     1681 acctcgacgg gcggtcgtca gtgttcgtcg tcgaggtggc gacgggcgcc gtcacgacga
     1741 tcgccggcgg gcccgacgcc gcgttcttca gccccacctg gaccgccaac gccgcatcga
     1801 tcctcgcgct cggcgaccgg ttcccgcgcg ccggctaccg gaccgggatc tggcgcttct
     1861 ccgccgacgg ctcggatgcc ggccggaccg gtggccttga cctgctcgc
//
LOCUS       DBNHXO010000007         3348 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2778943, whole genome shotgun sequence.
ACCESSION   DBNHXO010000007 DBNHXO010000000
VERSION     DBNHXO010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2778943"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="AC162A_00105"
     CDS             complement(<1..475)
                     /locus_tag="AC162A_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009272.1"
                     /translation="MIDQPTVRRPRTGASDLELFSLGWRRIQVLLRVRPPDAGFDPAL
                     VRIRHKATGAAVPLTGWEADEDCLTLRVNVMQGPDAEPLAPGRWELVVPGPRGGKGDP
                     VRLVGDAVAAGQSAPMSFAVGLRGTLNVVASVAARSGTFAIEVELDPTVAEPDEDA"
     gene            539..1990
                     /gene="ispD"
                     /locus_tag="AC162A_00110"
     CDS             539..1990
                     /gene="ispD"
                     /locus_tag="AC162A_00110"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIR5009273.1"
                     /translation="MQIHAIVLAGGDGNRFGGEMPKQFVRLAGDPILLRTLRALGASS
                     VDRVVVVSHPAWIDETRELVEAAALVMPVSVVAGGATRNESTRNGLAALDGADEDIVV
                     IHDAVRPLVPLDVIQRSIEPILSGRADGTDTVIPSADTLVIVEDDEVVEIPERSRYRR
                     GQTPQTFRLDVLARAYASATRAGDLSATDDCSLVLRHVPGVRIVAVAGDEVNVKITTR
                     IDMVMADRMLQMKVLTPIEEPEASASLEGMRLVVVGGTSGIGRAIADEAIRRGAHAEV
                     DGGSLGLDVRDYASVETRLGAAAERLGGIDHVVCTAGILRIGSVATTPAHELAEVVDI
                     NLTGTLNVARAAYPWLRASRGSLTVFTSSSFTRGRPDYVAYSASKAALVNMTQGLSEE
                     WADEGIRVNAVSPERTDTPMRRKAFPGESPEGLLGSEAVAVATLRLIQSDLTGQVLDV
                     RRHDALTPPLVVEPAEPGTVPVQPTGAPPARTR"
     gene            2016..3164
                     /locus_tag="AC162A_00115"
     CDS             2016..3164
                     /locus_tag="AC162A_00115"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF016357.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HIR5009274.1"
                     /translation="MSPLEYRLASLVLRVLVLFVRLLPVRRRRVVLATARLPLLEGNL
                     LHLHDAIRAIRPDVDVALLLQPYTYDLAGKLRYLARMVRGTYLVHTSGLVVVDNAYLP
                     VHVAPHREATTVVQVWHAPGALKRFGADTAGGLEEPEATFLHRYYDYVVAPGEGAREP
                     WSRALRTPVDQVLPLGTPRTDRFYDTAALDASRSWLLAIHPVLRDRKVVLYAPTFRGR
                     GRAKHGTGALNGRRLRERLPQEYALVLKSHPNLDPATQPVDGFDVVADPKSDMNDYLA
                     LASVFVTDYSSSIFEWAILHRPLVLVVDDLEAYEEDPGLYLDYRREMIGTQVRDADGV
                     ATAILADDFDVSGYDRFIAANVGACDGHASERFVDRFLPRHERVDRRS"
BASE COUNT          453 a         1232 c         1206 g          457 t
ORIGIN      
        1 tcgcgtcctc gtccggttcc gcgacggtcg ggtcgagctc cacctcgatc gcgaacgtgc
       61 ccgaacgggc ggcgaccgac gcgacgacgt tgagcgtgcc gcgcagcccc acggcgaacg
      121 acatcggggc ggattggccg gccgcaactg cgtcgccgac gaggcgcacc gggtcccctt
      181 tgccgcctcg cggtcccggc acgacgagct cccagcgacc gggtgccagc ggctcggcgt
      241 ccgggccctg catgacgttg acccgcaggg tcaggcagtc ttcgtcggcc tcccagccag
      301 tgagcgggac cgccgcgccg gtcgccttgt ggcggatccg gacgagcgcg ggatcgaacc
      361 cggcgtcggg tgggcggacc cggaggagga cctggatccg ccgccatccg agcgagaaca
      421 gctcaaggtc cgaggcgccg gtccggggtc ggcggacggt cggctggtcg atcatgtcgg
      481 ggaccgatga taccggtggg tgcgggccgg gctggccgcg ctgacgtagt ctctccgtgt
      541 gcagatccac gcgatcgtcc tcgccggcgg cgacggcaac cgcttcggcg gcgagatgcc
      601 aaagcagttc gtccggctcg cgggagaccc gatcctgctg cggacgctcc gcgcgctcgg
      661 ggcgtcgtca gtcgacaggg tggtggtcgt gtcgcacccg gcatggatcg acgagacgcg
      721 cgagctcgtc gaggccgcgg cgctggtgat gccggtcagc gtcgtcgccg gcggggcgac
      781 ccgcaacgag agcacgcgca acggcctggc ggccctcgat ggcgccgacg aggacatcgt
      841 cgtcatccac gacgcggtgc ggcccctggt ccccctcgac gtgatccagc gctccatcga
      901 gccgatcctg tccgggcggg cggacggcac ggacaccgtg atcccgagcg cggacacgct
      961 cgtgatcgtc gaggatgacg aggtcgtcga gatccccgag cggagccggt accgccgcgg
     1021 ccagacgccg cagacgttcc gcctggacgt cctcgcccgg gcctatgcgt cggcgacgcg
     1081 cgcgggtgac ctgtcggcga ccgatgactg cagcctggtc ctgcggcacg tgcccggcgt
     1141 gcggatcgtc gcggtcgcgg gcgacgaggt gaacgtcaag atcacgaccc ggatcgacat
     1201 ggtcatggcc gaccggatgc tccagatgaa ggtgctcacg ccgatcgagg agcccgaggc
     1261 cagcgcctcg ctcgagggga tgcggctggt cgtcgtgggc ggcacgagcg ggatcgggcg
     1321 cgcgatcgcc gacgaggcga tccgccgggg cgcgcacgcg gaggtcgacg gcgggtcgct
     1381 cggcctggac gtgcgcgact acgccagcgt cgagacgcgg ctcggggcgg ccgccgagcg
     1441 cctgggcggg atcgaccacg tggtctgcac cgccggcatc ctgcgcatcg ggtccgtcgc
     1501 caccacgccg gcccacgagc tcgccgaggt cgtggacatc aacctcaccg gcaccctcaa
     1561 cgtcgcccgg gcggcgtatc cgtggctgcg cgcgtcccgc ggctcgctca cggtgttcac
     1621 ctcgagctcg ttcacgcgcg gccgaccgga ctacgtggcg tactccgcga gcaaggcggc
     1681 gctggtcaac atgacccagg gcctgtccga ggagtgggcc gacgagggga tccgggtcaa
     1741 cgccgtgagc cccgagcgca ccgacacccc gatgcgccgc aaggcgttcc cgggcgagtc
     1801 acccgaggga ctcctgggct ccgaggcggt cgccgtcgcg acgctccgcc tgattcagtc
     1861 cgatctgacc ggccaggtcc tcgacgtccg ccgtcacgat gcgctgacgc cgccgctggt
     1921 cgtcgagccg gccgagcccg ggaccgtccc cgtacagccg accggcgcgc cgcccgcgcg
     1981 gacccggtag ccgtcgcccg cgtgggcgcc cgcccgtgag cccgctcgag taccgcctcg
     2041 cctccctcgt cctgcgcgtc ctcgtcctgt tcgtgcgcct gctgcccgtg cgccggcggc
     2101 gggtcgtgct cgcgacggcg cggctgccgc tgctcgaggg caacctcctc cacctccacg
     2161 acgcgatccg ggccatccgc cccgacgtcg acgtggcgct cctcctccag ccgtacacgt
     2221 acgacctggc gggcaagctg cgctacctcg cacggatggt ccggggaacc tacctcgtgc
     2281 acacgtcggg gctcgtggtc gtcgacaacg cctacctgcc ggtccacgtc gcgccgcacc
     2341 gcgaggcgac gacagtggtc caggtgtggc acgcacccgg agcgctgaag cggttcggcg
     2401 ccgacaccgc cggcggcctc gaggagcccg aagccacctt cctccaccgc tactacgact
     2461 acgtggtcgc gcccggggag ggcgcccgcg agccctggtc ccgggccctg cgcacgcccg
     2521 tcgaccaagt cctgccgctg ggcacgccgc gcaccgatcg cttctacgac acggcggcgc
     2581 tcgacgcgtc ccggtcgtgg ctgctggcga tccacccggt cctgcgggat cggaaggtcg
     2641 tcctctacgc cccgacgttc cgcggccggg gacgggccaa gcacgggacg ggcgcgctca
     2701 acggccgccg cctgcgcgag cgcctgccgc aggagtacgc cctggtcctc aagtcgcacc
     2761 ccaacctcga ccccgcgacc cagcccgtcg atggcttcga cgtcgtcgcg gaccccaaga
     2821 gcgacatgaa cgactacctc gcgctggcca gcgtgttcgt cacggactac tcgtcgtcga
     2881 tcttcgagtg ggcgatcctc caccggccgc tcgtgctggt ggtcgatgac ctcgaggcgt
     2941 acgaagagga cccgggcctg tacctcgact accggcggga gatgatcggc acccaggtcc
     3001 gggacgccga cggcgtggcc acggcgatcc tggccgacga cttcgatgtg tccggctacg
     3061 accggttcat cgccgccaac gtcggcgcct gcgacgggca cgcctctgag cgcttcgtcg
     3121 accgcttcct gcctcgccac gagcgggtgg atcgccggag ctgacggcgc cggctgcgtc
     3181 agcgggcgcc gagctcgagg gactccagga agccgtcgag gatcgcgcgg tcgccggcgt
     3241 cgtcgcgggt cgtcgactcg agcgagagca ccaggccgag gccgtcgttc accacggcgg
     3301 cgtcgaggta gtggatgagg tacgacggcg ggatcggcag gtgatagg
//
LOCUS       DBNHXO010000008         1870 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2779017, whole genome shotgun sequence.
ACCESSION   DBNHXO010000008 DBNHXO010000000
VERSION     DBNHXO010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2779017"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="AC162A_00120"
     CDS             <1..902
                     /locus_tag="AC162A_00120"
                     /inference="COORDINATES: protein motif:HMM:NF014879.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
                     /protein_id="HIR5009275.1"
                     /translation="LVEPLYGAGSGWIPRLLAGGRIRVTEVHNDRNPYFGGINPEPIR
                     PNVDEALALIAAGGYDLGLLLDGDADRAGAADERGTFLHQLEVTSLLMYYLAEYRGMR
                     EPVVVSVNNTSTAARLGKHYGIETHEVPVGFKFIGPKMIETGAMLGAEESGGFGFGMH
                     LPERDGIYADLCLLDLFLREREGGRAPVSTAMELFHELAGPSFYRRIDVHFDRPSYDA
                     VKRRVLVGLRESPPSSLAGTAIDRTQWLDTGDGVKWFLADGSWLLVRASGTEPLVRVY
                     TEATTAALRDELVVAGERLVRGG"
     gene            899..1282
                     /locus_tag="AC162A_00125"
     CDS             899..1282
                     /locus_tag="AC162A_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5009276.1"
                     /translation="MSEPEAKAGQRYVPKPWGHELIWALTDRYCGKLIAIDAGRRLSL
                     QRHQRKDESVYVLSGRLHLHLEDDDGVIQVIDLGPGESRHVPPGRIHRFEAREAVEVI
                     EVSTPEIDDVERLEDDYGREGTSAP"
     gene            1477..1755
                     /locus_tag="AC162A_00130"
     CDS             1477..1755
                     /locus_tag="AC162A_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009277.1"
                     /translation="MHSSLIGKVEKANRYARELDRISIERIALTFRGDNDTHHVSLDA
                     GQWHCTCHYFESWGSCVHLLTLQKIFGVMLPEDAQVSIFNASAAAALV"
BASE COUNT          278 a          666 c          656 g          270 t
ORIGIN      
        1 tgctggtgga gccgctgtac ggcgccgggt ccggctggat cccgcgcctg ctcgccggtg
       61 gccgcatccg cgtcaccgag gtccacaacg accgcaaccc gtacttcggc ggcatcaacc
      121 ccgagccgat ccgtcccaac gtcgacgagg cgctcgcgct gatcgccgcc ggtggctacg
      181 acctcggcct gctgctcgac ggcgacgcgg accgggccgg tgcggccgac gagcgcggca
      241 cgttcctgca ccagctcgag gtcacgagcc tgctcatgta ctacctcgcc gagtaccggg
      301 ggatgcgcga gccggtggtc gtgtcggtga acaacacgtc gacggcggcg aggctcggga
      361 agcactacgg gatcgagacc cacgaggtcc cggtgggctt caagttcatc gggcccaaga
      421 tgatcgagac cggggcgatg ctgggcgccg aggagtcggg cggcttcggg ttcgggatgc
      481 acctgcccga gcgcgacggc atctacgcgg acctgtgcct gctcgacctg ttcctccgtg
      541 agcgcgaggg cggccgggcg cccgtgtcga cggcgatgga gctgttccac gagctcgcgg
      601 gaccgtcgtt ctatcgtcgg atcgacgtcc acttcgaccg gccctcgtac gacgcggtga
      661 agcgccgcgt gctcgtcggc ctgcgcgagt cgccgccgag ctcgctcgcc gggaccgcga
      721 tcgaccggac ccagtggctg gacaccggcg acggcgtcaa gtggttcctc gcggacggct
      781 cgtggctgct cgtccgggcg agcgggaccg agcccctggt ccgcgtctac acggaagcca
      841 ccaccgccgc cctgcgcgac gagctggtcg tggccggcga gcgcctggtg cgcggcggat
      901 gagcgaaccg gaggcgaagg ccggccagcg gtacgtcccc aagccgtggg gccacgagct
      961 gatctgggcg ctgaccgacc ggtactgcgg caagctcatc gcgattgacg cgggtcgccg
     1021 gctgtcgctc cagcgccacc agcggaagga cgagagcgtg tacgtcctgt ccggccggct
     1081 ccacctgcac ctcgaggacg acgacggcgt gatccaggtg atcgacctcg gccccggcga
     1141 gtcgcgccac gtcccgcccg ggcggatcca ccggttcgag gcgcgcgagg cggtcgaggt
     1201 gatcgaggtc tcgacgcccg agatcgacga cgtcgaacgc ctcgaggacg actacggccg
     1261 cgagggaacg tccgctccgt gagctttcgc ccacgggacc gtggcgccca cgcaccgtcc
     1321 gacagtaccg gcgtcagccg gtccgattcc gggggctgac cgacggcccg ctggggtgta
     1381 ggcttgatgc agagaccgcc accaccggtc cctggtccga ccggacggcg gcccctcgtg
     1441 ggacgcccat tcggggcgac tggaggttga tcggcgatgc actcctctct catcggcaag
     1501 gtcgagaagg cgaatcgcta cgcacgcgag ctggaccgga tttcgatcga gcgaatcgcc
     1561 ctgacgttcc gcggtgacaa cgacacccat cacgtcagcc tcgatgcggg ccagtggcac
     1621 tgcacgtgcc actacttcga gagttggggt tcctgtgtcc acctcctcac gctgcagaag
     1681 atcttcggcg tgatgctgcc cgaggacgcc caggtctcga tcttcaacgc gtcggcggcg
     1741 gccgccctgg tctgagccgg ccggccacga ggccggccca gcgcaccgca tcagccatcg
     1801 acggcgaccc gccagtacca ggccgcccgg cggccgtcgc cgaacctcgc ctccagctgc
     1861 aggctccacg
//
LOCUS       DBNHXO010000009         4761 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4168509, whole genome shotgun sequence.
ACCESSION   DBNHXO010000009 DBNHXO010000000
VERSION     DBNHXO010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4168509"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..427)
                     /locus_tag="AC162A_00135"
     CDS             complement(254..427)
                     /locus_tag="AC162A_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009278.1"
                     /translation="MIDRRAPSGAGDTGPGILETSIVLGLALLLAAVIVVAFGGTLAD
                     AIGVLVDAAHGAS"
     gene            complement(424..1584)
                     /locus_tag="AC162A_00140"
     CDS             complement(424..1584)
                     /locus_tag="AC162A_00140"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019350.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5009279.1"
                     /translation="MSKTTDPPPASAAQLAADLGADVAAIDNELAEIDMLVGQARAEA
                     GRHEQKRAQLAAKLTATDEGGAPDAWAQLVTLTKRSVLMEAQVDVLEGKRRALARHRD
                     ALVVVAEAITVLAGESGAVPDGGPPSLSRVVMSAQEDLRREIARAMHDGPAQSLTNIV
                     LQAAIVERLLGRDPDSARGELRLLVSMVQQTLDATKNFIFEVRPMVLDDLGVVPTLRR
                     MVRDRGRRAKIPVEFETLGQDRRLPMDVESTVFRILDVALAAYLSLGPERVQVRLDWT
                     DELEARVIAERAPKLPGDGEALPDIPTDDMPDAIREMIMQRHEDRKAAVEAAEAAAIV
                     ELPAAVLRDVTDRASSIGGRVEMLAGGGELRVLVPLPRESDATTAGGALRPS"
     gene            complement(1614..2357)
                     /locus_tag="AC162A_00145"
     CDS             complement(1614..2357)
                     /locus_tag="AC162A_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5009280.1"
                     /translation="MTEYQGPERRRGGSDRRNQSYERVRIVIVDDHALFRVGIRQILE
                     REPDFEIVGEADDARSAMDAAFATNPDVILMDLSLPSPGGIETTQRVKRELPAAAIVV
                     LSTEEDEDALFDAIKAGAAAFILKDIAPEDLVMIIRRVVAGEYLINDKVFARPAVASR
                     VLKEFRELAVYGQEAAPIFAPLSPREVEILDNIAQGMTNKQVAYALSISEQTVKNHMS
                     SILRKLSVNDRTQAVVYAMRQGWIRMPED"
     gene            complement(2354..3463)
                     /locus_tag="AC162A_00150"
     CDS             complement(2354..3463)
                     /locus_tag="AC162A_00150"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5009281.1"
                     /translation="MDGPRGEPAESRYDELHAEAAEALSFAANTLRGIRDRYRQAYLD
                     EVTRSEDLNDDLAARERAADGGSGGAAVTSSDPTSLEAAQAAEAGAADLQLRLVRADA
                     GRSTDALGRHEGELSHLELAIRNLETTWLFIERGDASLVTDLDMPLLPTDLQMRIVEA
                     QEAERVRLAQEIHDGPAQALSNAIFQVEFVDRVFETDPSTAREELRLLRDLLRRALGD
                     VRTFIGQLRPPVLDELGLDGSIAETVSNQAALSGLLITTQLEAPAGDIPPATQTVVLR
                     VVQEALQNVRKHASATNAVVASMLDGGEWVLEVRDDGRGFDTGAVAARGRRNFGLQFM
                     RERAELIGARFEVHSRPEAGTVVRLAIPMTRKESG"
     gene            complement(3490..>4761)
                     /locus_tag="AC162A_00155"
     CDS             complement(3490..>4761)
                     /locus_tag="AC162A_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009282.1"
                     /translation="AVGSGLTAAVYLPLLLHSALPDSTMLFGALVLGAIVLMTRVLRE
                     PRGGRILDPRLLIIGLLIGLAALTRNEAIWLALTWAWLAFRRAGVDGRTRLRLVGVVA
                     VVSLLVFAPWAARDWAVFGNPLPGQAATNALSISGFDIFAWNEPPTLARYLGVGAGGL
                     LEMRSAGLAHNLFNVLLFLGIPVSLLGLVALPWQGRDRALRPLVLIAVLTFFATSLLF
                     PVATTWGTFLHASAPAAVLLIVSAVGLLDAGLAALGRRMGWERPIAWLGPLLTVFASA
                     LFSVALLPAFGAGAQATQRTYEVLEREMAAIGAPLDGSSPVIHDFPIWMAETARVPTL
                     ALPDESPSDVLDLATHFGAKWLIVGPEHGRWPAVLDGPDPDAACFEEVLLPIPADRLD
                     ADAIRDLRVFRIACAGVAGATAPESAVRPSP"
BASE COUNT          710 a         1685 c         1712 g          654 t
ORIGIN      
        1 gcccaggaag atgagggcga cgatcgcgac gatcgcgatg agggcgagga tcagcgcgta
       61 ctcggcgagt ccctggccct cctcgtcccg ctcgagcgag gcgaggaacg aggtgatgag
      121 cgccatatat gtgcctcccg atgagtgcca ccctgaggtc cagggcggcg ataacgtact
      181 cgctgggatc ggggattctc aatgagacga tggtcatggt cagcgcacgg aatcgcggtg
      241 ccaggaggaa cctttaggag gccccgtggg cggcgtcgac gagcacgccg atggcgtccg
      301 ccagcgtccc gccgaaggcc acgacgatga ccgcggccag gagcagcgcg agcccgagca
      361 cgatcgacgt ctcgaggatc cccggaccgg tgtcgcccgc gccgctcggg gcacgccggt
      421 cgatcacgac ggccgcaggg cgccgcctgc cgtcgtcgcg tcgctctcgc gcgggagcgg
      481 gacgaggacc cggagctccc cgccgccggc gagcatctcg acccggccgc cgatggacga
      541 cgcccgatcg gtcacgtcgc gcagcacggc cgcgggcagc tcgacgatcg ccgcggcctc
      601 ggcagcctcg accgccgcct tacggtcctc gtggcgctgc atgatcatct cccggatcgc
      661 gtcgggcatg tcgtcggtcg ggatgtcggg gagcgcctcg ccgtcgcccg gcagcttggg
      721 tgcccgctcg gcgatgacgc gggcctccag ctcgtcggtc cagtcgaggc gtacctggac
      781 gcgctcgggc ccgagtgaca ggtaggccgc gagggccacg tccaggatcc ggaagaccgt
      841 gctctccacg tccatcggca ggcgccggtc ctgccccagc gtctcgaact ccaccgggat
      901 cttggcccgc cgcccgcggt cgcgaaccat gcgccgcagc gtcggcacca cgccgaggtc
      961 gtcgaggacc atgggccgga cctcgaagat gaagttcttg gtcgcgtcga gcgtctgctg
     1021 gaccatcgac acgagcagcc ggagctcgcc ccgggcgctg tccggatcgc ggcccaggag
     1081 tcgctcgacg atcgccgcct ggagcacgat gttcgtcagc gactgggcgg gcccgtcgtg
     1141 catggcccgc gcgatctcac ggcgcaggtc ctcctgcgcg ctcatcacga cgcgcgagag
     1201 cgacggcgga cctccgtcag ggaccgcgcc ggactcgccg gcgaggacgg tgattgcctc
     1261 ggcgacgacc accagcgcgt cccgatggcg cgcgagcgcg cggcgctttc cctcgaggac
     1321 gtcgacctgt gcctccatca gcaccgaccg cttggtcagc gtgacgagct gggcccacgc
     1381 gtcgggcgct ccgccctcat cggtcgccgt cagcttggcg gcgagctggg cgcgcttctg
     1441 ctcatgtcgt ccggcctcgg cccgcgcctg cccgacgagc atgtcgatct cggccagctc
     1501 gttgtcgatc gccgcgacat ccgcgccgag gtcggcggcg agctgggccg cggaagccgg
     1561 cggtggatcg gtggtcttgg acatcgagcg cgcttcgtcc ggtctgcctc gcctcagtcc
     1621 tccggcatcc ggatccagcc ctggcgcatc gcgtacacga cggcctgggt ccggtcgttg
     1681 accgacagct tccggaggat ggagctcatg tggttcttga ccgtctgctc cgagatcgac
     1741 agcgcgtagg cgacctgctt gttggtcatg ccctgcgcga tgttgtccag gatctcaacc
     1801 tcgcgcggtg acaacggcgc gaagatcggg gcggcctcct ggccgtacac ggcgagctcg
     1861 cggaactcct tgagcacccg cgaggcgacc gccggccggg cgaacacctt gtcgttgatc
     1921 aggtactcac cggccacgac ccggcggatg atcatcacga ggtcttcggg tgcgatgtcc
     1981 ttgaggatga acgccgccgc gcccgccttg atggcgtcga acagggcgtc ctcgtcctct
     2041 tcggtggaca gcaccacgat ggccgcagcc ggcagctcgc gcttgacccg ctgcgtggtc
     2101 tcgatgccgc ccggggacgg cagggagagg tccatgagga tgacgtcggg gttggtcgcg
     2161 aacgcggcgt ccatcgcgga gcgtgcgtcg tccgcctcgc ccacgatctc gaagtccggc
     2221 tcccgctcca ggatctggcg gatgccgacc cggaacaggg cgtggtcgtc gacgatgacg
     2281 atccgtaccc gttcatacga ctggttgcgc ctgtccgagc cgcctctacg gcgctcggga
     2341 ccctggtact cggtcatccg ctctcctttc gcgtcatggg aatagccagc cggacgaccg
     2401 tgcccgcctc tggcctcgaa tggacttcaa agcgcgcgcc gatgagctcg gcccgctcgc
     2461 gcatgaactg gagcccgaag ttgcgacgac cgcgggccgc caccgcgccg gtgtcgaacc
     2521 cgcgaccatc gtcgcggacc tccaacaccc attctcctcc gtccagcatc gacgcaacca
     2581 ccgcgttcgt cgccgaagcg tgcttgcgga cattctgcag ggcctcctgc acgacgcgca
     2641 gcacgacggt ctgggtcgcc ggggggatgt cccctgccgg ggcttcgagc tgggtcgtga
     2701 tcagcagccc ggacagggcg gcctggttgc tcacggtctc ggcgatcgac ccgtcgagcc
     2761 ccagctcgtc gaggaccggc ggccggagct ggccgatgaa ggtgcggacg tcgcccagcg
     2821 cccggcgcaa caggtcgcgc aggaggcgca gctcctcgcg ggccgtcgag gggtccgtct
     2881 cgaacacgcg atcgacgaac tcgacctgga agatcgcgtt cgagagcgcc tgggccggtc
     2941 cgtcgtgtat ctcctgggcg aggcgcacgc gttcggcctc ctgggcctcg acgatgcgca
     3001 tctggaggtc ggtcgggagc agcggcatgt cgaggtccgt gaccagcgag gcgtcgccgc
     3061 gctcgatgaa cagccaggtc gtctcgaggt tgcggatcgc gagctcgagg tgcgacagct
     3121 cgccctcgtg gcgaccgagc gcgtccgtgg agcgcccggc gtcggcgcgg accaggcgca
     3181 gctgcaggtc agcggcgccg gcctccgcgg cctgggcggc ctcgagggag gtcgggtcgg
     3241 acgaggtgac ggcggctccg cctgagcccc cgtcggcggc gcgctcgcgc gcggcgaggt
     3301 cgtcgttgag gtcctccgac cgggtgacct cgtcgagata ggcctggcga tagcggtcgc
     3361 ggatgccgcg cagcgtgttc gccgcgaagc tcagcgcctc cgccgcctcg gcgtggagct
     3421 cgtcgtagcg actctctgca ggctcgcccc gaggaccatc catcgcgcgg agtgtatcgg
     3481 ccacacccat cacggcgacg gcctcacggc agattccggt gctgtcgcgc ccgcgacacc
     3541 cgcgcacgcg atccggaaca cgcggaggtc gcgaatggcg tcggcgtcga gccggtcggc
     3601 cgggatcggc aacaggacct cctcgaagca ggccgcatcg gggtccggtc cgtcgagcac
     3661 cgccggccag cggccgtgct ccggcccgac gatcagccac ttcgccccga agtgggtcgc
     3721 gaggtcgagg acgtcggacg gcgactcgtc cggcagcgcg agcgttggca cgcgcgccgt
     3781 ctcggccatc cagatcggga agtcgtggat cacgggcgac gagccgtcga gcggcgcgcc
     3841 gatcgcggcc atctcgcgtt ccagcacctc gtaggtccgc tgcgtcgcct gcgccccggc
     3901 gccgaaggcc ggcagcagcg ccaccgagaa cagggcggag gcgaacaccg tgagcaacgg
     3961 ccccagccag gcgatcggcc gttcccagcc catccgccgg ccgagggcgg ccagcccggc
     4021 atcgagcaac ccgaccgcgg acacgatcag cagcacggct gccggggccg acgcgtggag
     4081 gaacgtgccc cacgtcgtcg cgaccgggaa cagcaggctg gtggcgaaga acgtgagcac
     4141 ggcgatcaac accaggggcc gcagcgcccg gtcgcgaccc tgccacggca gggcgacgag
     4201 gcccaggagc gagaccggga tgccgaggaa cagcaggacg ttgaacaggt tgtgcgcgag
     4261 gccggccgag cgcatctcca gcagtccccc cgccccgacg ccgagatagc gcgccagcgt
     4321 cggtggctcg ttccaggcga agatgtcgaa cccggagatc gagagggcat tggtggccgc
     4381 ctggcccggc agcgggttgc cgaacacggc ccagtcgcgg gccgcccagg gcgcgaacac
     4441 gagcaggctg acgacggcga ccacgccgac cagccgcagg cgcgtccggc cgtccacgcc
     4501 ggcgcggcgg aacgccagcc aagcccaggt gagcgcgagc cagatcgcct cgttgcgcgt
     4561 gagggccgcg agcccgatga gcaggccgat gatgagcagg cggggatcga ggatccgacc
     4621 gcccctcggc tcgcgcagca cgcgggtcat gagcacgatc gcgcccagga cgagcgcgcc
     4681 gaacagcatc gtcgagtcgg gcagggccga gtggagcagc agcggcaggt agacggccgc
     4741 cgtgaggccg gatccgacgg c
//
LOCUS       DBNHXO010000010         2785 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1192336, whole genome shotgun sequence.
ACCESSION   DBNHXO010000010 DBNHXO010000000
VERSION     DBNHXO010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1192336"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="AC162A_00160"
     CDS             complement(<1..143)
                     /locus_tag="AC162A_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013495.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HIR5009283.1"
                     /translation="MIASVDGVVGAISTDSLVIEVGGIGYRVFVAPSVIATAPAGGRL
                     RLHT"
     gene            complement(151..810)
                     /gene="ruvC"
                     /locus_tag="AC162A_00165"
     CDS             complement(151..810)
                     /gene="ruvC"
                     /locus_tag="AC162A_00165"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR5009284.1"
                     /translation="MIVLGIDPGTAALGYGIVDRTASGLRAIDYGCLTTSADLPLPER
                     LVRIHDLVAELIETHHPSIVAVERLFFSKNVQTAFAVGQARGVVLLAAAQAGVPVREA
                     TPNEVKVGVTGYGRAEKGQVARMVTVILRLADVPTPDDAADALAIAISCANAERPGDR
                     LYGTAKGATAAVMDRAAVAPIVRGETPYERSVREALAREKAAAPASPAKAAAPTARRQ
                     P"
     gene            complement(807..1556)
                     /locus_tag="AC162A_00170"
     CDS             complement(807..1556)
                     /locus_tag="AC162A_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR5009285.1"
                     /translation="MSGHSKWSTIKRQKGVNDSKRSAMFTKVAREIAVAARAGGGDPD
                     ANYRLRLAIDKARSINMPADNIKRGIEKATGGGDDVQYEEIVYEGYGPGGVAVLVEAA
                     TDNRNRTAAEVRSIFAKSGGQLAGSGAVAWQFEPRGLITVDREGTDPDEVALAGIDAG
                     ATDVDTEVDPIEIYTDPGDLERIRKSLEAAGVNVEGAENAMIAKQTVSLDASHVRQAL
                     RLVELLEDLDDVQRVTANVDIPEELLAEVAG"
     gene            1614..>2785
                     /locus_tag="AC162A_00175"
     CDS             1614..>2785
                     /locus_tag="AC162A_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012239.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR5009286.1"
                     /translation="MTVKSHDLAHIRSVVLAGHAGAGKTTLAEQVLFRAGAIPRQGRV
                     DDGTAHLDYEPEEQKRHQSLSLAVGTFEHGEHEITVVDTPGYPDFTAEMIQGFHAADA
                     ALFVVDASGGVEAGLEAAVTTGRATGTAACFVLNKCDRENADPGAALDALREAFGTKI
                     APLHLAIGAADTFSGYVDLVHRKAWRFEGGKEVEIPVPDELADEIARRRDQLLEAAAE
                     ADDAVFEKYLGEEDISDAELDACLHKGVRESILAPVLVASAAKGIGLSALLDAIVRYL
                     PSPEEEGPYAATDKAGKELEVPATGNQLLVRVFKTAADPFVGRLTYMRVLSGTLKSQG
                     TVFNSSRGEDERIGQLLYLHGKEQEPAPELRAGEIGAVAKLGVTETGDTITTRDAAL"
BASE COUNT          405 a          991 c          989 g          400 t
ORIGIN      
        1 gtgtgcaacc gcaggcggcc gcccgccggt gcggtcgcga tcaccgacgg tgcgacaaac
       61 acccggtaac cgatgccgcc gacctcgatg acgagcgaat cggtggagat cgcgccgacc
      121 acgccatcga ccgacgcgat catcggcgcc ctacggctgg cggcgggcgg tgggggcggc
      181 cgccttggcc ggggacgcgg gcgcggcggc cttctcgcgg gcgagggcct cgcggaccga
      241 gcgctcgtac ggcgtctcgc cacgcacgat cggggcgacg gccgcgcggt ccatgaccgc
      301 cgcggttgct cccttcgccg tcccgtacag gcggtcgccc ggccgctcgg cgttcgcgca
      361 cgagatcgcg atcgcgagcg catcggcggc gtcgtcgggg gtcggcacgt cggccagccg
      421 caggatcacc gtgaccatcc gcgcgacctg gcccttctcg gcccggccgt acccggtgac
      481 gccgaccttg acctcgttgg gcgtcgcctc gcggaccggc acgcccgcct gcgccgcggc
      541 gagcagcacg acgccccgcg cctggcccac cgcgaacgcg gtctgcacgt tcttcgagaa
      601 gaacaggcgc tcgacggcga cgatcgacgg gtggtgggtc tcgatcagct cggccacgag
      661 gtcgtggatc ctgacgagcc gctcgggcag cggcaggtcc gcgctcgtcg tcaggcaccc
      721 gtagtcgatc gcgcgcagcc ccgacgcggt ccgatcgacg attccgtagc ccagcgcggc
      781 ggtcccgggg tcaatcccca ggacgatcac ccggcgacct cggccagcag ctcctcgggg
      841 atgtccacgt tggccgtcac gcgctggacg tcgtcgaggt cctccagcag ctcgaccagg
      901 cgcagggcct ggcgcacgtg gctcgcgtcc aggctgaccg tctgcttcgc gatcatcgcg
      961 ttctcggcgc cttcgacgtt cacgcccgcc gcctcgagcg acttgcggat gcgctcaagg
     1021 tcgcccggat ccgtgtagat ctcgatcgga tcgacctcgg tgtcgacgtc ggtggctccg
     1081 gcatcgatgc ccgccagcgc gacctcgtcg ggatcggtgc cctcgcgatc gacggtgatc
     1141 aggccgcgcg gctcgaactg ccaggcgacg gccccggaac ccgcgagctg gccgccggac
     1201 ttggcgaaga tcgagcgcac ctccgccgcc gtgcggttgc ggttgtcggt tgcggcctcg
     1261 acgaggaccg cgacgccgcc cgggccgtag ccctcgtaca cgatctcctc gtactggacg
     1321 tcgtcaccgc cgccggtcgc cttctcgatc ccccgcttga tgttgtcggc gggcatgttg
     1381 atcgaacgcg ccttgtcgat ggcgagccgc agccggtagt tcgcgtccgg gtcaccgccg
     1441 ccagcgcgtg cggcgacagc gatctcgcgc gcgaccttcg tgaacatggc gctgcgcttc
     1501 gagtcgttga cgcccttctg gcgcttgatc gtcgaccact tggaatgtcc ggacatcggc
     1561 cgggagtata ccgatatcat cgaatcccga gtcaccacga ggtaccccgc gcgatgaccg
     1621 tcaagagcca cgacctcgcc cacatccgct ccgtcgtgct cgcgggccac gccggagctg
     1681 ggaagacgac gctcgcggag caggtcctct ttcgagccgg cgcgatcccc cgccagggcc
     1741 gggtcgacga tggcacggcg cacctggact acgagcccga ggagcagaag cgccaccagt
     1801 ccctgagcct cgccgtcggg acattcgagc acggcgagca cgagatcacg gtcgtcgaca
     1861 cgccaggcta tccggacttc acggcggaga tgatccaggg cttccacgcg gccgatgcgg
     1921 cgctgttcgt cgtcgatgcc tcgggcggcg tcgaggccgg gctggaggcg gccgtgacaa
     1981 cgggtcgggc gacgggcacg gccgcgtgct tcgtgctcaa caagtgcgac cgcgaaaacg
     2041 ccgatccggg cgccgcgctg gacgcgctgc gcgaggcgtt cgggacgaag atcgcgccgc
     2101 tgcacctcgc gatcggcgcc gcggacacgt tcagcggcta cgtcgacctc gttcaccgca
     2161 aggcgtggcg gttcgagggc ggcaaggaag tcgagatccc tgttccggac gagctcgccg
     2221 acgagatcgc gcgtcgccgg gatcagctgc tcgaggcggc cgcggaggcg gacgatgccg
     2281 tgttcgagaa gtacctgggc gaggaggaca tctcggacgc cgagttggac gcgtgcctcc
     2341 acaagggcgt tcgcgagtcg atcctcgcgc cggtgctcgt cgcgtcggcg gccaagggca
     2401 tcgggctgtc ggccctgctc gacgcgatcg tgcgctacct gccctcgccc gaagaggagg
     2461 gcccgtacgc ggcgacagac aaggcgggca aggagctcga ggtgccggcg accggcaacc
     2521 agctcctcgt acgcgtgttc aagaccgccg cggacccgtt cgtcggccgg ctgacgtaca
     2581 tgcgcgtcct gtcgggcacg ctcaagagcc aggggacggt gttcaactcc agccgcggcg
     2641 aggacgagcg catcggccag ctgttgtacc tccacggcaa ggaacaggag ccggcgcccg
     2701 agctgcgggc gggcgagatc ggggccgttg ccaagctcgg ggtgaccgag accggcgaca
     2761 cgatcacgac gcgcgacgcg gcgct
//
LOCUS       DBNHXO010000011         2216 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4764422, whole genome shotgun sequence.
ACCESSION   DBNHXO010000011 DBNHXO010000000
VERSION     DBNHXO010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4764422"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="AC162A_00180"
     CDS             complement(<1..526)
                     /locus_tag="AC162A_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR5009287.1"
                     /translation="MDLTVIIVIAIIVVIALFLVSLYNGLVTRRNRIDEAFAQIEVQL
                     KRRHDLIPNLIAAVKGYMGFEQSVLTQVTEARANAVAAGAQGPAQQASAENALTSSLR
                     SLFAVVENYPDLKANENVMRLQEELTTTENQISFSRQHYNATVLDFNNSIQTFPAVLI
                     AGPFGFHKREFFDAE"
     gene            complement(623..699)
                     /locus_tag="AC162A_00185"
     tRNA            complement(623..699)
                     /locus_tag="AC162A_00185"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(663..665),aa:Val,seq:tac)
     gene            complement(749..833)
                     /locus_tag="AC162A_00190"
     tRNA            complement(749..833)
                     /locus_tag="AC162A_00190"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(797..799),aa:Leu,seq:gag)
     gene            complement(873..958)
                     /locus_tag="AC162A_00195"
     tRNA            complement(873..958)
                     /locus_tag="AC162A_00195"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(923..925),aa:Leu,seq:cag)
     gene            complement(990..>2216)
                     /locus_tag="AC162A_00200"
     CDS             complement(990..>2216)
                     /locus_tag="AC162A_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5009288.1"
                     /translation="TPIAGFLAAATQPLLPAHLLDAVPFAELATSSFAREPVGTGPYV
                     LTELDDTHAVLLPTALVNATEVLPTEPAPSMDALGTPSPTSTPTLPVPYLERIEVDFF
                     DDDAAAAAAFAADDVDAVAGLPPASSANLSTAPGVDRVRYPTTTLATVMLNLRASHPE
                     LRDARVRRALLGAVDRDALVADALGGDGIAADTLVPPTSWAFDAAAAAPVKFDTKAAA
                     KLLTEAGWKKKNDAWTAKDAKAPFRMQLLTVPPAANPRLAAVTRSLKEAWEDFGIGVD
                     IVETPASELATKLRTGAFTAAALDITTGLEPDLYPLLASSQVRGSGTNLTGYQDPALD
                     TLLEAARKPGTAEARTAAWKALLTGLAARQPLLPIAWHSESVLFQGVNGPTARLIAGP
                     GDRFWDVLAWRLAADR"
BASE COUNT          334 a          729 c          812 g          341 t
ORIGIN      
        1 gctcggcgtc gaagaactcg cgcttgtgga agccgaacgg accggcgatc aggacggcag
       61 gaaacgtctg gatcgagttg ttgaagtcca gcacggtggc gttgtagtgc tgacgcgaga
      121 agctgatctg gttctcggtc gtcgtcagct cctcctggag gcgcatgacg ttctcattcg
      181 ccttgaggtc ggggtagttc tcgacgacgg cgaacagaga gcggagggac gacgtcaggg
      241 cgttctcggc ggaggcctgc tgggccggac cctgggcacc cgcggcgaca gcgttggcgc
      301 gcgcctcggt gacctgggtg agcacgctct gctcgaaccc catgtagccc ttgacggcgg
      361 cgatcaggtt cgggatcagg tcgtggcggc gcttgagctg gacctcgatc tgcgcgaacg
      421 cctcgtcgat gcggttccgt cgtgtgacca gcccgttgta caggctcacg aggaacagcg
      481 cgatcacgac gatgatcgcg atgacgatga tcacggtcag atccatccgg tctcctcgag
      541 tcctctcggg cacacgcccc gcgtcagcat accgcgccgg ctcctcgccc accgcaagac
      601 gcctcggcgc gcaatgtcgg agtggtgggc ggtggaggac tcgaacctcc gacttcctcg
      661 gtgtaagcga ggcgctctaa ccaactgagc taaccgcccc ggaaacgacg agcgggcggc
      721 tcgtgccgcc cgcggagggt caggagagtg gtgcccaggc cgggacttga acccggatga
      781 gttgcctcat acgcccctca aacgtacgcg tataccaatt ccgccacctg ggcctgtgtt
      841 cgcttgccga ccggcacgtc ggtgaccgtt cctggtaccg agggagggac tcgaacccac
      901 acgaccttgc gatcactagg tcctgaacct agcgcgtcta ccattccgcc acctcggccc
      961 ggccgaccac cgatcggacg cccgagggct taccggtctg cggcgaggcg ccatgctagc
     1021 acatcccaga accggtcccc ggggcccgcg atgagcctcg ccgtgggtcc gttgaccccc
     1081 tgaaacagca cgctttcgct gtgccaggcg atcggcaaca ggggctgccg cgcggcgaga
     1141 ccagtgagca gggccttcca cgcggcggtg cgcgcctcgg cggttccggg cttgcgcgcg
     1201 gcctcgagca gcgtgtcgag cgcagggtcc tggtagccgg tcaggttggt ccccgagccg
     1261 cggacctggc tcgacgccag cagcgggtag aggtcgggct ccaggccggt ggtgatgtcg
     1321 agggcagccg cggtgaaggc tcccgtgcgc agcttggtgg ccagctccga cgcgggtgtc
     1381 tcgacgatgt cgaccccgat cccgaagtcc tcccaagcct ccttgagcga ccgggtgacg
     1441 gcggccaggc gcgggttggc cgcgggcggg acggtcagga gctgcatccg gaacggcgcc
     1501 ttggcgtcct tggccgtcca tgcgtcgttc ttcttcttcc agcccgcttc ggtgagcagc
     1561 ttcgccgccg ccttcgtgtc gaacttgacg ggcgccgcgg cggcggcgtc gaaggcccac
     1621 gacgtgggcg gcacgagcgt gtccgcggcg atgccgtcgc cgccgagcgc atcggcgacc
     1681 agcgcgtcac ggtcgacggc gccgaggagc gcgcgtcgca cgcgcgcgtc gcgcagctcg
     1741 ggatgcgagg cgcgcaggtt gagcatgacc gtcgcgagcg tggtcgtcgg atacctgacc
     1801 cgatcgacgc cgggcgccgt ggacaggttg gccgacgacg cgggcggcag gccggcaacc
     1861 gcgtcgacgt catcggcggc aaacgcggcc gccgcagcag cgtcgtcgtc gaagaagtcg
     1921 acctcgatcc gctcgaggta gggcaccggg agggtcggcg tcgacgtcgg cgacggcgtg
     1981 ccgagcgcgt ccatcgacgg cgcgggctcg gtcggcaaga cctcggtggc gttgacgagg
     2041 gcggtcggga gcagcaccgc gtgggtatcg tcgagctccg tgaggacgta cggcccggtc
     2101 ccgaccggct cccgcgcgaa ggagctggtg gcgagctccg cgaacgggac ggcgtcgagc
     2161 agatgcgccg ggaggagcgg ttgggtcgcc gccgcgagga agcccgcgat gggcgt
//
LOCUS       DBNHXO010000012         5632 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3574116, whole genome shotgun sequence.
ACCESSION   DBNHXO010000012 DBNHXO010000000
VERSION     DBNHXO010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5632)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3574116"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="AC162A_00205"
     CDS             complement(<1..708)
                     /locus_tag="AC162A_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR5009289.1"
                     /translation="MKLGVIIPQGWTGDYDGWDATAAWNRSVALARQADDLGVESIWV
                     FDHFHTVPRPTDEITFESFTMLSALAAVTTRVRLGHIVICTAFRNPALTAKMISTMDV
                     ISGGRMELGIGAGWKRDEWDAYGYGFPETRERLARLADDLEVVSRMMDGDRHQHATFA
                     GTYSSVTDARNVPKPVQRPRVPIMVGGNGPNVTWRLAAKFADELNLDGMDPSDVAAAL
                     PTVRARCEEIGRDPATLA"
     gene            770..1147
                     /locus_tag="AC162A_00210"
     CDS             770..1147
                     /locus_tag="AC162A_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019250221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HIR5009290.1"
                     /translation="MHDKARAAVERLAHGPEIAYLFWPTVMAYLRIATHPSVFERPLS
                     IPVAIGNVEQLIGRPHVRTTGETGAFWLSFRDVTDDALPSGNLVPDAHLVTLMLDNDV
                     RTIWTRDRDFRRFPRIEVRDPFA"
     gene            1181..1696
                     /locus_tag="AC162A_00215"
     CDS             1181..1696
                     /locus_tag="AC162A_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR5009291.1"
                     /translation="MVVIFGWGSGDAQDLGEVAPVVCPNCHNQVLLHHIRSDQRVTLY
                     FIPLASYGTNDYLSCPICRHGLQIPPEQREAVERMQSATRVFRSGALSAALYQPQVEA
                     FWQRFGVAPSGEQVVHPAPTMPAPAAVPSAAAPASPTLAEQLEGYAKLHKDGVLTDEE
                     FVALKRKVLGI"
     gene            complement(1707..2888)
                     /locus_tag="AC162A_00220"
     CDS             complement(1707..2888)
                     /locus_tag="AC162A_00220"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR5009292.1"
                     /translation="MSHPRISAKAASFTESVIREMNRLAVEAGAVSLAQGFPDFACPQ
                     ELKDAAAKAVHDDINQYAITWGATNLREAIATSTVEQFPGWSEIDPATMITVTCGATE
                     AMVAAMLALLDPGDEVVIFEPYYENYGPDAIISGAVPRYVTLHEPDWSIDPDELRAAF
                     GPRTRGIVLNSPHNPTGKVFSREELALIAALCVEFDVIAFTDDIYEHIVFEGEHIPLA
                     TMPGMADRTVSIHSMSKSYSVTGWRVGWTIASPDLTVGIRRVHDFLTVGAAAPLQAAA
                     VVALGFPATYYEHLVADYAARRDALLPSLREAGFRFVEPAGAYYVMTDIRGLTDEDDT
                     TFALRLIREVGVAAVPGSSFYSRPELGRAKLRFAFPKRLETLEVAAARLLANRPVEAA
                     G"
     gene            complement(2898..3359)
                     /locus_tag="AC162A_00225"
     CDS             complement(2898..3359)
                     /locus_tag="AC162A_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009293.1"
                     /translation="MDYMYGAELEAERAGWYEFAELVRSLTPGECLIPGYYHDPDWAV
                     RDLAGHIGTWLAEAASQLERIAVGTYEGHDIDVDSLNAQFLAAMDGMAWDVAWLQANA
                     GRTRLMSAWSKLRSPNDEAAWWIRKSGGDHYAEHLPHLRDWVGELMARRAA"
     gene            complement(3388..3564)
                     /locus_tag="AC162A_00230"
                     /pseudo
     CDS             complement(3388..3564)
                     /locus_tag="AC162A_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            complement(3577..5328)
                     /locus_tag="AC162A_00235"
     CDS             complement(3577..5328)
                     /locus_tag="AC162A_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5009294.1"
                     /translation="MTLTVRQRVEEQETGLLGRETELGLLRLALAPDGPLVVFVHGIG
                     GVGKTTLLEAFSAEARVAGAVVLRLDCGSIEPTARGFLDAISAATGSDLATAEDAAVR
                     LGSLGPRVILALDRYEVLRPLDLWLLQAFVPVLHDNVRVLLAGREGPMAGWSIAMRQL
                     FRSLPLGNLPRDDAEALLRRDGVGGDDVERIQRLARGHPLSLRLAASALVAGPSLDHD
                     ATTVTAIVTELTELYLERLDPQTRRALDAAAVVRRPTLSLMGAMLPDAAPQDVFDRLR
                     GLPFVELSSDGLVIHDTVREVVAAYLRATDPDRSRRYRIAAWRQLRDEVTRATSQEMW
                     RYTADLLYILENPAVREAFFPTSEHLYFVEPAIEGDWPAIHAIAMAAEPPEAVEILED
                     WWRRLPDAFWAARDGAGNIVGFSVMTQVDRVPRSLFDADPLARIFRDHVRRLPVPRGQ
                     RVLVLRFQFAHPADPDQALVIAALILDVKRQYMELRPELRRIYNVLPEVYGPGSPWTR
                     LGFRTVLDAPVVFGGVAYYAHVLDFGPASVDGWLTRIIATELQVDDDSILDVAQHQLV
                     LADRRVALTRLEFEVFQ"
     gene            complement(5358..>5632)
                     /locus_tag="AC162A_00240"
     CDS             complement(5358..>5632)
                     /locus_tag="AC162A_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009295.1"
                     /translation="GTYAGKILEYAPGPTTVITATYQFKGSERPFTALVHVEQTGLEG
                     VVRGVVTDGWGKGSPVHGGYTEIKCDHDGVTTDCWQGTLTIEDPGR"
BASE COUNT          853 a         1948 c         1998 g          833 t
ORIGIN      
        1 cgccagcgtc gcggggtcgc gcccgatctc ctcgcagcgc gcccggaccg tcggcagggc
       61 ggcggcgaca tcggacgggt ccatcccgtc gaggttcagc tcatcggcga acttcgcggc
      121 cagccgccag gtgacgttcg gtccgttgcc tccgaccatg atcggcacgc gcggccgctg
      181 caccggcttg gggacgttcc gggcgtcggt gacgctggag tatgtgcccg cgaaggtcgc
      241 gtgctggtgc cggtcgccgt ccatcatccg cgagacgacc tcgaggtcgt ccgccaggcg
      301 cgcgagccgc tcgcgcgtct cgggaaagcc gtagccgtag gcgtcccatt cgtcgcgctt
      361 ccagccggcg ccgatcccga gctccatccg gccgccgctg atgacgtcca tcgtcgagat
      421 catcttcgcc gtcagcgcgg ggttccggaa cgcggtacag atcacgatgt gccccaaccg
      481 gaccctcgtg gtcacggcgg ccagggcgga cagcatcgtg aacgactcga acgtgatctc
      541 gtcggtgggc cgggggacgg tgtggaagtg gtcgaagacc cagatcgact cgaccccgag
      601 gtcatccgcc tggcgggcga gggcgacgct gcggttccag gccgccgtcg catcccagcc
      661 gtcgtagtcg cctgtccacc cctgcgggat gatcacgccg agcttcatct ggttcgcctc
      721 cacgcgatgc gttcgcccag tatcgcccgg acgccgctgg acgagtccag tgcacgacaa
      781 ggcgcgtgcg gccgtggagc ggctcgccca cggaccggag atcgcctacc tcttctggcc
      841 caccgtgatg gcctatctcc ggatcgcgac gcacccgtcc gtcttcgagc gaccactctc
      901 gatcccggtg gccatcggca acgtcgagca gctgatcggc cgaccgcacg tacgcacgac
      961 cggcgaaacg ggcgccttct ggctgtcgtt tcgggacgtc accgatgacg cactgccgtc
     1021 gggcaatctc gttcccgacg cccacctcgt gacactgatg ctcgacaacg acgtacgcac
     1081 gatctggaca cgtgaccgcg acttccgccg cttcccccgc atcgaggttc gcgatccgtt
     1141 cgcctgagcg tgatacaacc ggcgcggagg cggaacgcga atggtcgtga tcttcgggtg
     1201 gggttcgggg gacgcgcagg acctgggcga ggtcgccccc gtcgtctgcc ccaactgcca
     1261 caaccaggtg ctcctgcacc acatccgctc ggaccagcgg gtcacgctgt acttcatccc
     1321 gctcgcgtcg tacgggacca acgactacct ctcgtgcccg atctgccggc acgggctcca
     1381 gatcccgcca gaacaacgcg aggccgtcga gcggatgcag tcggccaccc gggtgttccg
     1441 gagcggtgcc ctgagcgccg ccctctatca gccccaggtg gaggcgttct ggcagcggtt
     1501 cggcgtggca ccgtcgggcg agcaggtcgt gcatcccgcg ccgaccatgc ccgcgccggc
     1561 cgcggtcccg tccgcggccg cgccggcgtc gccgaccctc gccgagcagc tcgagggcta
     1621 cgcgaagctt cacaaggacg gcgtgctgac cgacgaggag ttcgtcgcac tcaagcgcaa
     1681 ggtcctcggg atctgaccgt ccgtcgctag ccggcggcct cgacggggcg gttggcgagc
     1741 aggcgggcgg cggcgacctc cagggtctcg agccgcttcg ggaaggcgaa ccgcagcttc
     1801 gcgcgcccga gttccgggcg cgagtagaac gacgagcccg gcactgccgc gacgccaacc
     1861 tcgcggatca ggcgcagcgc gaaggtcgtg tcgtcctcgt cggtcaggcc gcggatgtcg
     1921 gtcatgacgt agtaggcccc ggcgggctcg acgaaccgga agccggcctc gcgcagcgac
     1981 ggcagcaggg cgtcgcgcct cgccgcgtag tcggccacga ggtgctcgta gtaggtcgcc
     2041 gggaacccga gcgccacgac cgccgcggcc tggagcggcg ccgccgcgcc gaccgtgagg
     2101 aagtcgtgca cccggcggat cccgacggtg aggtcgggcg atgcgatggt ccagccgaca
     2161 cgccagccgg tgaccgagta cgacttcgac atcgagtgga tcgagaccgt ccggtcggcc
     2221 atgccgggca tcgtcgccag cgggatgtgc tcgccctcga acacgatgtg ctcgtagatg
     2281 tcgtcggtga acgcgatgac gtcgaactcg acgcacaggg cggcgatgag cgcgagctcc
     2341 tcgcggctga acaccttgcc ggtcgggttg tggggcgagt tgaggacgat gccccgcgtc
     2401 cgggggccga aggccgcgcg gagctcgtcg ggatcgatcg accagtcggg ctcgtggagc
     2461 gtcacgtagc gcgggaccgc gcccgagatg atggcgtcgg ggccgtagtt ctcgtagtag
     2521 ggctcgaaga tcacgacctc gtcgcccggg tcgaggagcg ccagcatcgc ggcgaccatg
     2581 gcctcggtcg ccccgcaggt cacggtgatc atcgtggccg ggtcgatctc gctccagccg
     2641 gggaactgct cgacggtcga ggtcgcgatc gcctcgcgca ggttcgtggc gccccaggtg
     2701 atcgcgtact ggttgatgtc gtcgtggacc gccttcgccg cggcgtcctt gagctcctgc
     2761 ggacaggcga agtcagggaa gccctgggcg aggctgaccg cccccgcctc gaccgcgagc
     2821 cggttcatct cgcggatgac cgattcggtg aagctggccg ccttggcaga gatccggggg
     2881 tggctcatcg ggggacgtca ggcggcacgc cgcgccatga gctcgccgac ccagtcccgg
     2941 aggtgcggga ggtgctcggc gtagtggtcg ccgcccgact tgcggatcca ccaggccgcc
     3001 tcgtcgttgg gtgaccgcag cttcgaccag gccgacatca ggcgcgtccg cccggcgttg
     3061 gcctggagcc acgcgacgtc ccaggccatc ccgtccatcg ccgcgaggaa ctgcgcgttg
     3121 aggctgtcga cgtcgatgtc gtggccttca tacgtgccga cggcgatccg ctcgagctgt
     3181 gaggcggcct cggcaagcca ggtgccgatg tgcccggcca ggtcgcggac cgcccagtcg
     3241 ggatcgtggt agtagccggg gatcaggcac tcgccgggcg tcagcgagcg aaccagctca
     3301 gcgaactcgt accagccggc ccgctcggcc tcgagctcgg cgccgtacat gtagtccatg
     3361 gcggccattg tcgccggtcg gccgcggcta cgtcgacgcg acgtaccggt agccgaggcc
     3421 gcgcacggtc gcgatcgagg atgcgcgatc gccgagcttg cgccgcagcg acttgatcag
     3481 ggcctcgatc acgttgctgc cgcccgcgta gtcgtagccc cagacgtcgc gcaggaggga
     3541 cgcccgttcc acgaccctgc cgggtcgctc gtagagctac tggaacacct cgaattccag
     3601 cctcgtgagc gcgacccgac gatcggccag gacgagctgg tgctgggcga cgtcgaggat
     3661 cgagtcgtcg tcgacctgca gctccgtcgc gatgatccgt gtcagccagc cgtcaaccga
     3721 tgccggcccg aagtcgagga cgtgcgcgta gtacgcgacg ccgccgaaca cgactggggc
     3781 gtcgagcacc gtccggaaac caagccgggt ccacggcgat ccaggcccgt agacctccgg
     3841 gaggacgttg tagatgcgtc ggagctcggg ccggagctcc atgtactggc gcttgacgtc
     3901 aaggatcagc gccgcgatga ccagggcctg gtcgggatcc gccggatgcg cgaactggaa
     3961 gcggaggacc aggacccgct gcccacgggg gacgggcagg cgccgaacgt ggtcgcgaaa
     4021 gatgcgcgcg agcggatcgg cgtcgaacag ggagcgaggg acgcgatcga cctgggtcat
     4081 cacggagaag cccacgatgt ttcccgcccc gtcacgcgcc gcccagaacg cgtcgggcag
     4141 gcgacgccac cagtcctcga ggatctcgac cgcctccggg ggctcggccg ccatcgcgat
     4201 cgcgtggatt gccggccagt cgccttcgat cgccggctcg acgaagtaga ggtgctcgga
     4261 cgtcgggaag aacgcctcgc gtaccgccgg gttctcaagg atgtagagga ggtcggccgt
     4321 gtagcgccac atctcctgcg aggtcgcgcg cgtgacctca tcgcggagct gccgccaggc
     4381 ggcgatccgg tagcggcggg accggtcggg atctgtcgcc cgcaggtacg ccgcgacgac
     4441 ctcgcggacg gtgtcgtgaa tgacgaggcc gtcgctgctc agctcaacaa acggcagccc
     4501 tcgcaggcgg tcgaacacgt cctgcggtgc cgcatctggc agcatcgcgc ccatcagcga
     4561 gagcgtcggc cgcctgacca ccgcggcggc atcgagcgcc cggcgcgtct gtggatcaag
     4621 gcgctcgaga tacagctccg tgagttctgt gacgatggcc gtcaccgtcg tcgcgtcgtg
     4681 gtcaagcgaa gggcccgcca cgagtgctga cgccgcgagg cgcaaggaga ggggatgccc
     4741 gcgggccagc cgctgaatgc gctccacgtc gtcgccccca acgccgtcac ggcggagcaa
     4801 ggcttcggcg tcgtcgcgcg gcaggttgcc cagtggcagg ctccggaaca gctggcgcat
     4861 cgcgatcgac cagccggcca tgggtccctc tcgaccggcg agcagcacgc gcacgttgtc
     4921 gtgcaggacc gggacgaagg cctgcagcag ccagaggtcg agcgggcgga gcacctcgta
     4981 gcggtcgagc gcgaggatga cccgcgggcc gaggctcccg aggcggacgg ccgcgtcctc
     5041 ggcggtcgcc aggtcgctcc ccgttgccgc tgagatggca tcgaggaaac ctcgcgccgt
     5101 tggctcgatc gacccacagt cgagacgaag gacgaccgcg cccgcgacgc gggcctccgc
     5161 cgagaacgcc tcgagcagcg tggtcttgcc gacgccgccg atgccatgga cgaagacgac
     5221 cagcggcccg tccggcgcca gggcgaggcg caggaggccc aactcggtct cgcggccgag
     5281 gagcccggtc tcctgctcct cgacccgctg gcggacggtg agcgtcatgt gcggagtctg
     5341 cgctgcacgt caacggctca gcgaccgggg tcctcgatgg tgagggtgcc ctgccagcag
     5401 tccgtggtga ccccgtcgtg gtcgcacttg atctccgtgt acccgccatg cacggggctg
     5461 cccttgcccc agccgtccgt gacgacgccg cggacaacgc cctcaaggcc ggtctgttcg
     5521 acgtggacga gcgccgtgaa cggccgctcg gagcccttga actggtacgt ggcggtgatg
     5581 acggtggttg ggccgggcgc gtactcgagg atctttcccg cgtacgtccc tt
//
LOCUS       DBNHXO010000013         3198 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4169751, whole genome shotgun sequence.
ACCESSION   DBNHXO010000013 DBNHXO010000000
VERSION     DBNHXO010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4169751"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="AC162A_00245"
     CDS             <1..380
                     /locus_tag="AC162A_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009296.1"
                     /translation="ERPGDWTHALMDVGATLCRPRSPLCPSCPASAWCGYAAGQRPSS
                     SATARAAARVPEPAFPATTRWLRGRIVARARDADDGDWVAFDAPIGGHDLSAVREAVV
                     ALASDGLIEVRESSAGPEARLPR"
     gene            421..1203
                     /locus_tag="AC162A_00250"
     CDS             421..1203
                     /locus_tag="AC162A_00250"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HIR5009297.1"
                     /translation="MPTETVPLPADDPDLQASTLDDLARRWAEAAGRGAITGEVMTGL
                     DRKAQASGTPGATLMEHAGTAVAAAAHALAVRNGRDDRPVLILAGPGNNGGDGFVAAR
                     RLAGWGIASIVVLVAADEKPGTRDAALNWKRLEGLEAVTRVHAAVARDVAILGQGIEK
                     AGVVVDALLGSGVSGRLREPVTTAVGVIGRARAAGVPVLAVDTPTALDLSSGEPSDPV
                     VRADLTVTFHRPKTGLQTKIGRALAGRILVAPIGISRDVDRA"
     gene            1205..1402
                     /locus_tag="AC162A_00255"
     CDS             1205..1402
                     /locus_tag="AC162A_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009298.1"
                     /translation="MRQPGVRDVLLVAAVAVGVVLLAAALTAVLPREGQAVVFHTPLL
                     IGVLVLGTAFVLWRILRAPRP"
     gene            1413..3080
                     /locus_tag="AC162A_00260"
     CDS             1413..3080
                     /locus_tag="AC162A_00260"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HIR5009299.1"
                     /translation="MTDAPDRPAPALPLERRNRDLARLANEPWDLLIVGGGIVGAGAL
                     LDAASRGLRVALVEQDDIASGTSSRSSRLIHGGLRYLQQFRIGLVREALDERARLLRL
                     APHLVRLEEFLFPLYGLPIVTRAFYTTGMTMYDVLGSAKSGGRHRQLSTDGALEYAPN
                     LRRAGLRGALVYHDAMEDDARYTLAVVRTAVAHAAGSALAVTRVRATAPLQEGDRVVG
                     AVLTDALTGATIEARATTILDATGVWASLPERPFGTGSISVLPSRGSHLVIPRDRIPA
                     RGGMTLRIPGRVAFLVPWPRHWLIGTTDKPYHGAVERPAASAEDVDEILGTVNGALDC
                     DLTRDDVVGTYAGLRPLIAPSGSTSTVQVSREHRVSVEGAGLVRVSGGKYTTYRVMAA
                     DAVDAVLGSTQAKRRPSATAELPLIGAATLADLATLAGRIAEAEGIGAAAASSLVDRH
                     GTKAEAVLAAGAAHGLTRPLAGGFPYLESEVAWAVEHELAQSLDDVLARRIRLAPELR
                     DRGAAVAPRVAEMMGDALGWDAARRAEEITTYLELAHRQFDVPEIAP"
BASE COUNT          375 a         1208 c         1195 g          420 t
ORIGIN      
        1 tggaacgccc gggcgactgg acccacgcgc tcatggacgt cggggcgacg ctgtgccggc
       61 cgaggtcgcc gctctgccct tcgtgtccag cgtctgcctg gtgcggatac gcggcgggcc
      121 agcgcccgtc gtcgtcggcg acggcccgcg ctgcggcccg agtccccgag cccgccttcc
      181 cggccacgac ccgctggctg cgcggccgga tcgtcgcccg ggcccgcgac gccgacgacg
      241 gtgactgggt cgcgttcgac gccccgatcg gcggccacga cctgagcgcc gtgcgcgagg
      301 cggtggtggc gctcgcctcc gacggcctga tcgaggtgcg cgagtcgagc gccggccccg
      361 aggcgcgcct gccgaggtag gccgccgatc gccccgggcg ccgcacggta ccctctccgc
      421 gtgcccaccg agacggttcc gctgcccgcg gacgaccccg acctgcaggc gtccacgctc
      481 gacgacctcg cccggcgctg ggccgaggcg gccggccgcg gcgcgatcac gggcgaggtg
      541 atgacggggc tcgatcgcaa ggcccaggcc agcggcacgc cgggtgcgac gctgatggag
      601 cacgccggga ccgccgtcgc cgccgcggcc cacgcgcttg ccgtccgcaa cggccgcgac
      661 gaccgtcccg tcctgatcct cgccgggcct ggcaacaatg gcggcgacgg gttcgtcgcg
      721 gcgcggcggc tcgccggctg gggaattgcg tcgatcgtcg tgctcgtggc cgccgacgag
      781 aagcccggca cgcgcgacgc cgcgttgaac tggaagcgcc tcgaaggcct ggaagccgtc
      841 acccgggtgc acgccgcggt cgcgcgggac gtcgcgatcc tggggcaggg gatcgagaag
      901 gccggggtcg tcgtcgacgc gctcctcggg tccggggtca gcgggcgcct tcgcgagccg
      961 gtgacgacgg cggtcggcgt catcgggcga gccagagctg ccggcgtccc ggtcctcgcc
     1021 gtcgatacgc cgacggccct cgacctctca tcgggcgagc cgtcggaccc ggtggtgcgc
     1081 gccgatctga ccgtgacctt ccaccggccg aagacggggc tccagacgaa gatcgggcgg
     1141 gcgctggcgg ggcggatcct cgtcgcgccg atcgggatct cgcgggacgt cgaccgtgcc
     1201 tgacgtgcgc cagcccggcg tccgcgacgt gctgctcgtc gctgcggtgg ccgtgggcgt
     1261 cgtcctgctc gccgccgcgc tcacggctgt cctgccgcgc gagggccagg cggtcgtgtt
     1321 ccacacgccg ctgctgatcg gcgtgctcgt gctcgggacg gcgttcgtcc tgtggcgcat
     1381 cctgcgcgcg ccacgaccct gacgcggccg cgatgaccga cgcgcccgat cgcccggcgc
     1441 ccgcgttgcc gctcgagcgg cgaaaccggg acctcgcgag actcgccaac gagccctggg
     1501 acctgctgat cgtcggcggt gggatcgtcg gggcgggcgc tctcctcgat gccgcctccc
     1561 gcgggctgcg cgtcgcgctg gtggagcagg acgacatcgc ttccgggaca tcctcgcgct
     1621 ccagccgcct gatccacggc ggcctgcgct atctccagca gttccgcatc gggctcgtcc
     1681 gcgaagcgct cgacgagcgg gcccgcctgc tccgcctcgc gccgcacctt gtccggctgg
     1741 aggagttcct gttcccgctc tacgggctgc cgatcgtcac ccgcgccttc tataccaccg
     1801 gcatgacgat gtacgacgtg ctggggtcgg cgaagtcggg tgggcggcac cgccagctgt
     1861 cgacggacgg ggcgctcgag tacgcgccga acctgcggcg cgcgggtctc cggggtgcgc
     1921 tcgtctacca cgacgcgatg gaggacgacg cgcgctacac gctggcggtc gtccggaccg
     1981 cggtcgccca cgcggcgggc tcggccctcg cggtcacgag ggtgcgggcc acggccccgt
     2041 tgcaggaagg cgaccgcgtc gtcggggccg tcctgaccga tgcgctcacc ggagcgacga
     2101 tcgaggcgcg cgcgacgacg atcctcgatg cgacgggcgt ctgggcgtcg ctgccggagc
     2161 ggccgttcgg gacgggctcg atcagcgtcc tgccgtcgcg cggcagccat ctcgtgatcc
     2221 cccgcgaccg gatcccggcc cgcggcggca tgaccctgcg gatcccgggg cgggtcgcgt
     2281 tcctcgtccc gtggccgcgc cactggctga tcggaaccac cgacaagccg taccacggcg
     2341 cggtcgagcg gcctgcggcg agcgcggagg acgtcgacga gatcctcggg acggtgaacg
     2401 gggcgctgga ctgcgacctg acgagggacg acgtggtcgg cacgtacgcc gggctccggc
     2461 ccctgatcgc gccgtcaggc tcgacctcga cggtgcaggt gtcgcgcgag caccgggtca
     2521 gcgtcgaggg ggcggggctc gtccgcgtca gcggcggcaa gtacacgacc taccgggtga
     2581 tggcggccga cgccgtcgac gccgtgctgg gctccaccca ggcgaagcgg cggcccagcg
     2641 ccacggccga gctgccgctg atcggcgcgg cgacgctggc cgacctcgcg acgctcgccg
     2701 gcaggattgc cgaggcggaa ggcatcggag cggcggccgc gtcgtccctc gtggaccggc
     2761 atgggacgaa ggccgaggcg gtcctcgccg ccggcgcagc ccacggtctc acccggcccc
     2821 tcgccggcgg cttcccgtac ctcgagtcgg aggtcgcctg ggcggtcgag cacgaactcg
     2881 cccaatctct cgacgacgtc ctcgcccggc gcatccgcct ggcgcccgag ctccgcgacc
     2941 gcggtgcggc ggtcgcgccg cgcgtggccg agatgatggg cgacgccctc ggctgggacg
     3001 cggcccgccg ggccgaggag atcaccacct acctcgagct cgcccatcgc cagttcgacg
     3061 ttccggagat agccccatga cggacggccg cgtgatcctc gcgctcgacc agggcacgac
     3121 gtcgtcgcgc gccatcgctt tcgaccacgc cgggacgccc gtcgcgagcg cccagcgcga
     3181 gttcccccag ggcttccc
//
LOCUS       DBNHXO010000014         6862 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2582012, whole genome shotgun sequence.
ACCESSION   DBNHXO010000014 DBNHXO010000000
VERSION     DBNHXO010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6862)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2582012"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="AC162A_00265"
     CDS             complement(<1..919)
                     /locus_tag="AC162A_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5009300.1"
                     /translation="MPGRVVSRRFIGREAELARVVDAVTAAASGAATTVIVSGSAGMG
                     VTRFLDEALDRVSGRPQPPMVLRGQSRGPVDPPWAAVLDAIAPLLADRPDAELDALLA
                     RDGRPILLGLPGMVERAQHVPGPRASDLADPERRQPRALESLLRWLGRVAAERPIVIV
                     LEDLHAADAATRSFATFVARIARSERICLVLTFQPDQPDRLTLEHPLRENLAVIEAGL
                     RPPVRIDLAPLARREIAGLIEGIDGERPSASIVVLVAERSAGSPLVVEELVAARRELR
                     SATLTGSLADLVGARLARRSPECRRVLRLL"
     gene            984..1946
                     /locus_tag="AC162A_00270"
     CDS             984..1946
                     /locus_tag="AC162A_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HIR5009301.1"
                     /translation="MSERRAPHPANGLNELPGEEWLYFTKSVWTTAYPSELGHARRRA
                     HGANKPPRLMARLIEFFTRTDELVLDPFAGVGGTLLGAAIARGPRRAIGIELDPRWRT
                     VFEETLAALLAEHGGTGPRLADLGPADPGGQRPFDPSGVELRLGDARALLPTLPAASI
                     DFVATDPPYNVQLPMTMAGGKLAETHANRRTDYAMRSDLAGDLANLPDYAAFLDAMSE
                     VLCELHRVLRPGRYVVLIVRDAYQEGRYLFTASDLAARAAAEGLVPKGDITWYQAGTR
                     LRPYGYPRTFVPNIVHQHILVLRKPPAPRAKPSRRRVVPAETGS"
     gene            complement(1943..3631)
                     /locus_tag="AC162A_00275"
     CDS             complement(1943..3631)
                     /locus_tag="AC162A_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009302.1"
                     /translation="MRHHRTPVGALVTALGRPSVRAALASLLLVVALITPASAATDSH
                     LQNPDVSPGSGHPTTTITFSITYRSQYGTKPDWVRVKVGGKTIDMASDGGTDWRDGVR
                     FSATSKLDVGEWPTQFVTQLRDRWTFVTDGPDVTIEPEPTPKPTPKPTPKPTPKPTPK
                     PTPKPTPKPTPKPEPKPEPRSNPTPRPTHQPDPKPDKPDPTAKPTPKPDPTNEPTQAA
                     ATTSPNGAPSLEPNAAGWPAGYESPDSQYPVEGASPSADTLAPSPSPDPVTAGVILGA
                     GAAGGTPGDPGGHGPNAGGSGGGGSTLPDGGAAGGTTPGDEPGSLGQAPGILAILAAV
                     MPTAVVTTGAVTMLMAFMVFGKRRRDGEPTADDDILAAQAARGTGAVPNSGTVGSKVM
                     PGAVAAATAVQAAAAPPPELDQIDGHLPRWRRPSLMQARKTDPTRSVSTGLKLTFDGE
                     AGSAVTGMERRLIRYRLVSLLSAPDEVRGVEIGVLDEGDEVILLEKAGTYWRVLCPDG
                     REGWLHKMTLGDVVIDSSAAGAGTWTSADDGPILGGFEDVMRAYRERRAEFGEA"
     gene            3940..4695
                     /locus_tag="AC162A_00280"
     CDS             3940..4695
                     /locus_tag="AC162A_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009303.1"
                     /translation="MQILAGPNQITRSDRIAIARSAFTSGLLVIVGLLVGWLCLGTTL
                     VTAFVPNGRPSVAEAATSVVAWAFAIVVPAAFLIIGFARIAAIIDTWSSMRPRTATAR
                     LVKSLGPEYMGATGVHVPGGRRLHELVLGPFGILVIAVVPPSKITRHVGSRWEIRDER
                     GRWLPIEAPLDRASRDAERVRSWLASDDRDFLVRVYAAIVTEERGIERTSACAVVAPR
                     ELAGWIAALPAQRGLSAERRQGLAETIAEATHG"
     gene            complement(4737..4813)
                     /locus_tag="AC162A_00285"
     tRNA            complement(4737..4813)
                     /locus_tag="AC162A_00285"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4777..4779),aa:Ile,seq:tat)
     gene            complement(4866..5177)
                     /locus_tag="AC162A_00290"
     CDS             complement(4866..5177)
                     /locus_tag="AC162A_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009304.1"
                     /translation="MTNEQTAHIALPKLYGAPAYARPPVLKVVTADRPFDPDSLPLEN
                     EQTEEERELTVELAATSSFGAAISDALASSRDGSELRGRPFQLTAIKRRIRRDGKITR
                     R"
     gene            complement(5299..5718)
                     /locus_tag="AC162A_00295"
     CDS             complement(5299..5718)
                     /locus_tag="AC162A_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009305.1"
                     /translation="MTGPSADPRPTVEVRRRGWFEVRWRQFRRAPRPVFRAVVASLTV
                     ATLLGCAFLAYDIALDRGARLPGGDLRLLFLTAYVAIVVAAGALITWLIVPLPTGSGT
                     RRATRTPWSAALGLFAAVPIAYLVLVVLHEIVKPLLT"
     gene            complement(5699..>6862)
                     /locus_tag="AC162A_00300"
     CDS             complement(5699..>6862)
                     /locus_tag="AC162A_00300"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR5009306.1"
                     /translation="AGTAGPMPSETQRAMDEQAQRELATGRAHPDMLPEILERMGEAR
                     AALAAVIVAGGDDVALTHSVSDGLNLFVNALPWAAGDRVLTTRHEHPGGLGPLLALRD
                     RLGVELEALDIGDGGDDERTLAAFTAALERPARAILVSHVLWTTGAVLPIRRLGELAR
                     SAGVVMLVDGAQAAGATPVSVEELAVDAYAMAGHKWLLGPEGMGALWVRRDLADSLTP
                     AHATFLSYQTFDIEHPVLQPGARRFEATSFHRPSVVGLARSCGWISMYVGLPWAHERA
                     ARLAGAAADRLASIPGVSLVTPRKRMTTLVTFRIAGWPAATAVAELGSRAFAILRDLP
                     MLDALRISVGFWTTEEELDRFAEAVELLAAHSPESIPSRRTLTILGSDDRPLR"
BASE COUNT          916 a         2461 c         2542 g          943 t
ORIGIN      
        1 cgagcagtcg caacacgcga cgacactcgg gcgaccggcg cgcgagccgg gcgcccacca
       61 ggtccgcgag cgagcccgtg agcgtggcgc tgcgcagctc gcgccgtgcc gcgaccagct
      121 cctcgaccac cagtggcgac ccggcggacc gttccgcgac gagcacaacg atggacgcgg
      181 acggtcgctc tccgtcgatc ccctcgatga gcccggcgat ctcgcggcgg gccagggggg
      241 caagatcgat ccggaccggc ggccggaggc ccgcctcgat caccgcgagg ttctcgcgca
      301 gcgggtgctc gagggtgagc cgatctggct ggtcgggctg gaaggtgagc acgaggcaga
      361 tgcgttcgct gcgggcgatc cgggcgacga acgtcgcgaa cgagcgggtg gcagcgtcgg
      421 ccgcgtgcag gtcctcgagc acgatcacga tcggccgctc cgccgcgacc cggccgagcc
      481 accgcagcag cgactcgagg gcccggggct ggcgccgctc cgggtcggcg aggtcgctgg
      541 ctcgtgggcc cgggacgtgc tgtgcacgct ccaccatccc ggggagcccg agcaggatcg
      601 gccgcccgtc ccgggccagc agggcgtcca gctcggcgtc cgggcgatca gcgaggagcg
      661 gcgcgatcgc atcgagcacc gcagcccagg gcggatcgac cggtccgcgg gactggccgc
      721 ggagcaccat cggtggctgc gggcgaccgg acacgcgatc gagcgcctcg tcgaggaatc
      781 gcgtcacgcc catcccggcg ctgcccgaca cgatcacggt ggtggcggcg cctgaggctg
      841 cggcggtgac ggcgtcgacg actcgggcga gctccgcctc ccggccgatg aaccgtcgac
      901 tgaccacgcg tcccggcatg ccgcattgta gggccgccgc ctcggtctcc cgggcaggtc
      961 gtcgcgccgg gtacgctgcc ccggtgagcg agcgccgggc accccacccc gccaatggcc
     1021 tgaacgagct ccccggcgag gagtggctct acttcacgaa gtcggtgtgg acgacggcct
     1081 atccgtccga gctcggtcac gcgcggcgcc gggcccacgg cgccaacaag ccgcctcgcc
     1141 tgatggcccg cttgatcgag ttcttcaccc gcaccgacga gctggtcctg gatccgttcg
     1201 ccggcgtcgg cggaacgctc ctgggggccg ccatcgcgcg aggtccgaga cgggcgatcg
     1261 ggatcgagct ggatccccgt tggcgaacgg tgttcgagga gaccctcgcc gcgctgctcg
     1321 ccgagcacgg tggcacggga cctcgcctcg ccgacctcgg gccggccgac ccgggcgggc
     1381 aacggccgtt cgacccgtcc ggcgtggagc ttcgcctcgg cgacgcaagg gcgctgctgc
     1441 ccacgctccc ggccgcatcg atcgacttcg tcgccaccga cccgccgtac aacgtgcagc
     1501 tgccgatgac gatggccggc gggaagctcg cggagaccca cgccaaccgg cggacggact
     1561 atgcgatgcg ctccgacctc gcgggcgacc tcgccaacct gcccgactat gccgccttcc
     1621 tcgacgcgat gagcgaggtc ctgtgcgagc tccatcgcgt gctgcgcccg ggccggtatg
     1681 tcgtgctcat cgtccgcgac gcgtaccagg agggccggta cctgttcacc gcctcggacc
     1741 tggcggcgcg ggccgcggcg gaggggctcg tgcccaaggg cgacatcacg tggtaccagg
     1801 cgggaacccg gctgcggccc tacggctacc cacgaacgtt cgtgccgaac atcgtgcacc
     1861 agcacatcct ggtgctgcgc aagccacctg caccgcgggc gaagccgtcc cggcggcggg
     1921 tcgtcccggc cgagacgggg agctaggcct cgccgaactc cgcgcgacgc tcccggtaag
     1981 cgcgcatgac gtcctcgaag ccgcccagga tcggtccgtc atcggccgac gtccaggtgc
     2041 cagcgcccgc cgctgacgag tcgatcacga catcgcccag ggtcatcttg tgcagccagc
     2101 cctcgcgccc gtccgggcac aggacccgcc agtaggtccc ggccttctcg agcaggatga
     2161 cctcgtcgcc ctcgtcgagg accccgatct cgacgccgcg gacctcgtcc ggcgcgctga
     2221 ggaggctgac caggcggtag cggatcagcc ggcgctccat ccccgtgacc gcgctgccgg
     2281 cctcgccgtc gaaggtgagc ttgaggccag tcgacaccga cctcgtcggg tcggtcttgc
     2341 gcgcctgcat cagcgacggg cggcgccagc gcgggaggtg gccgtcgatc tggtcgagct
     2401 cgggcggagg ggccgcggcc gcctgcacgg cggtcgccgc ggcgacggca cccggcatca
     2461 ccttggagcc caccgtcccc gagttcggca cggcgcccgt tccacgcgcg gcctgggcgg
     2521 cgaggatgtc gtcgtccgcg gtgggctcgc cgtcgcgccg gcgcttcccg aacaccatga
     2581 acgccatcaa catcgtgacg gcgccggtcg tgacgaccgc tgtcggcatc accgcggcga
     2641 ggatggcgag gatgccgggc gcctggccga gcgagccggg ctcgtcgccg ggcgtggttc
     2701 cgccggcagc gccgccgtcc ggcagggtgg atccgccgcc cccgctgccg cccgcattgg
     2761 ggccatgtcc accaggatcg ccaggggtcc cgccggccgc accagctcca aggatgacgc
     2821 cggccgtcac cgggtccggc gaaggcgacg gcgcaagggt gtcggcggac ggggacgcgc
     2881 cctcgaccgg gtactgcgag tcggggctct cgtagccggc gggccagccc gccgcattgg
     2941 gctcgagcga cggcgcgccg ttcggagacg tcgtcgcagc ggcctgcgtc ggctcgttcg
     3001 tgggatccgg cttcggggtc ggcttcgcgg tcgggtccgg cttgtctggc ttcgggtccg
     3061 gctggtgggt cggtcggggg gtcgggttcg acctgggttc gggcttgggc tcgggcttgg
     3121 gggtcggctt cggcgtcggc ttgggggtcg gcttcggcgt cggcttcggg gtgggcttcg
     3181 gcgtgggctt cggggtgggc ttcggggtgg gctcgggctc gatcgtgacg tcgggtccat
     3241 cggtgacgaa cgtccagcgg tcgcggagct gggtcacgaa ctgggtcggc cactcgccga
     3301 cgtcgagctt cgacgttgcc gagaagcgga cgccgtcgcg ccagtcggtg ccgccgtccg
     3361 acgccatgtc gatggttttg ccgcccacct tgacgcgcac ccagtcgggc ttcgtgccgt
     3421 actggctgcg gtaggtgatc gagaaggtga tcgtcgtcgt gggatgcccg ctgccgggcg
     3481 agacgtccgg gttctggagg tggctgtcgg tcgctgccga ggccggggtg atcagcgcga
     3541 cgacaagcag cagcgacgcc agcgcggccc ggaccgacgg tcggcccagc gcggtgacga
     3601 gcgcgccaac gggtgtccgg tgatgccgca accaggtagt cccactcggg tccctcaggg
     3661 cgtgagggct cttcgtggag catcggagcg ccgatcccgt gccgcaatgg tacgggggtc
     3721 ctcagcgtgt tgggaccggc ggtgcggcaa cgccccgcac cggacccccg ccggcccgca
     3781 gctacgtgtc aggtggcggg ccgaagattc cggtgcaccc ttgcaccacc ccgcgggcag
     3841 tctggtgcgg tccggtgtgg agaaccggac ggtcgccgcc tcccgggtag cccgatgtgg
     3901 tcactcgccg cgagcgcgcg tggcggcgac catcggggca tgcagattct cgccggaccc
     3961 aaccagatca cccgctcgga ccggatcgcc attgcgcgct ccgccttcac gtcgggactt
     4021 ctggtcatcg tgggcctgct cgtcggctgg ctgtgcctcg ggacgaccct cgtcaccgcc
     4081 ttcgtcccga acggccgccc gtccgtcgca gaggccgcca cgagtgttgt cgcgtgggcg
     4141 tttgccatcg tggttcccgc cgcgttcctg atcatcggct tcgcccggat cgcggcgatc
     4201 atcgatacct ggtcgtcgat gcggccgcga acggcgaccg cccgcctcgt caagtcgctg
     4261 ggtccggagt acatgggcgc gaccggtgtc cacgtcccgg gcggccgccg gctgcacgag
     4321 ctcgtgctcg gaccgttcgg gatcctcgtg atcgccgtcg tgccgccgtc caagatcacc
     4381 cgccacgtcg gcagtcgctg ggagatccgc gacgagcgtg gtcggtggct cccgatcgag
     4441 gctccgctcg accgcgcgtc gcgcgacgcc gagcgcgtcc gcagctggct cgccagcgac
     4501 gatcgcgact tcctcgtccg cgtctacgcc gcgatcgtca ccgaggagcg cgggatcgaa
     4561 cggacatctg cctgcgccgt cgtcgcgccg cgcgagctcg cgggctggat cgccgcgctg
     4621 ccggcccagc ggggtctgtc agccgaacgg cgccaggggc tcgccgagac catcgcggag
     4681 gcgacccacg gctgagcgca cgagcgggcg cggctgcgtc gacgcacgag cgagggtggt
     4741 aggggagcca ggactcgaac ctggaaccgg cgggatataa gcccgcagct ctaaccgatt
     4801 gagctactcc cccacggcgg ccgggccggt gcgggcgcgg ccgtcaggcc gtgagcgagc
     4861 gccggtcagc gccgggtgat cttgccgtcg cgccgtatcc ggcgcttgat cgctgtgagt
     4921 tggaacgggc gaccccgcag ctccgagccg tcgcgtgacg acgccagcgc atcgctgatc
     4981 gcggcaccga acgaggacgt cgccgcgagc tcgacggtga gctcgcgctc ctcctcggtc
     5041 tgctcgttct ccagcggcag cgagtccgga tcgaacggcc gatccgccgt cacgaccttc
     5101 aggacaggcg ggcgagcata cgcaggggca ccgtagagct tcgggagcgc gatgtgtgcg
     5161 gtctgttcgt tggtcacgag cgtcctcggt cgccgactcg ttgtcggact gcggggatca
     5221 taccgggcct gaaaaggtcc gtctcgctac ccgaaaggcc catggtcgta ctccggggtg
     5281 aggccaacgc gcaccgggtc acgtcagcag gggtttcacg atctcgtgga gcaccacgag
     5341 gacaaggtac gcgatcggta ccgccgcgaa cagcccgagc gcggcgctcc atggcgtccg
     5401 cgtggcgcga cgtgtcccgg acccggtggg cagcgggacg atgagccagg tgatcagcgc
     5461 gccggcggcg accacgatcg ccacgtaggc ggtgaggaac agcagccgca ggtccccgcc
     5521 gggcagccgg gcgccccggt cgagcgcgat gtcgtacgcg aggaacgcgc agcccagcag
     5581 cgtcgcgacc gtcaggctcg cgaccacggc ccggaagacg ggtcgcggtg cgcggcggaa
     5641 ctggcgccag cggacctcga accagccgcg ccgccgaacc tcgaccgtcg gccgggggtc
     5701 agcggagggg ccggtcatca ctgcccagga tcgtgagcgt ccgtcgcgac gggatgctct
     5761 cgggggagtg ggccgccagg agctcgacgg cctcggcgaa ccggtccagc tcctcctccg
     5821 tcgtccagaa gccgacggag atccggagcg cgtcgagcat cggcaggtcg cgcaggatgg
     5881 cgaacgcccg cgagccgagc tccgcgacgg cggtcgcggc cggccagccg gcgatgcgga
     5941 acgtgaccag cgtcgtcatc cgctttctcg gcgtcaccag gctcaccccg gggatcgacg
     6001 cgagacggtc ggcggcggca ccggccagcc gtgcggcgcg ctcgtgggcc cagggaagtc
     6061 ccacgtacat cgagatccag ccgcagctcc gggcgagccc gacgaccgac ggccggtgga
     6121 agctcgtggc ctcgaaccga cgggcgccgg gttggagcac ggggtgctcg atgtcgaagg
     6181 tctggtacga gaggaacgtg gcgtgcgccg gcgtcagcga gtcggcaagg tctcgacgga
     6241 cccacagggc gcccatcccc tcggggccca ggagccactt gtggccggcc atcgcgtagg
     6301 cgtcgacggc gagctcctcg accgagacgg gcgtcgcccc cgcggcctgc gcgccgtcta
     6361 cgagcatcac gacaccggcc gatcgcgcca gctcgccgag ccgccggatc ggcagcacgg
     6421 cgccggtggt ccagagcacg tggctgacca ggatcgcgcg cgccggccgc tcgagggcgg
     6481 cggtgaaggc ggcgagggtc cgttcgtcgt cgccaccgtc accgatgtcg agcgcctcca
     6541 gctcgacgcc cagacggtcg cgcagcgcga gcagcgggcc tagcccgcca ggatgctcgt
     6601 ggcgggtcgt caggacgcga tcgccggccg cccacggcag cgcgttcacg aacaggttga
     6661 ggccatcgct gacgctgtgg gtcagcgcga catcgtcgcc gcccgcgacg atcaccgccg
     6721 cgagggccgc ccgcgcctcg cccatgcgct cgaggatctc cggcagcatg tccgggtggg
     6781 cccgcccggt cgcgagctcc cgctgtgcct gttcgtccat cgcccgctgg gtctcggacg
     6841 gcatcggccc cgcggtcccg gc
//
LOCUS       DBNHXO010000015         3393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_400188, whole genome shotgun sequence.
ACCESSION   DBNHXO010000015 DBNHXO010000000
VERSION     DBNHXO010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_400188"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1224
                     /locus_tag="AC162A_00305"
     CDS             91..1224
                     /locus_tag="AC162A_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5009307.1"
                     /translation="MPASDRGPATPTTPELVLAQRMHALGFLADGVAHDLANPLGAIL
                     GLASLIAADERVPDDLRTDARLLREEADRTYRLVSTLLDVTRTRPPVLRPTAVAPTVT
                     SLLELATHPLAGVTVEVAIASDLPEVEVDPSAFRYAIAGLVVDALERLGAPSASGRLR
                     IEAAATGEGPRATVRLSLIDDGRPVEPGATTVTGIGALLDRLGVPVIQDTIDAAGHPR
                     NRTLLDLRVPIQGEAWPAAGVGTTPGSPDAGDPRRPLVLVCDDEASVRALLVRVLERA
                     GCEAIEAGSGEEALLALDQAAVDAIVSDHHLPAMTGIDLYERLAATRPDLRQRFVLLS
                     GDPDSPEIVAFIARSGVPIVAKPFDLAGIEATIRAVVLPVTGG"
     gene            complement(1225..2085)
                     /locus_tag="AC162A_00310"
     CDS             complement(1225..2085)
                     /locus_tag="AC162A_00310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5009308.1"
                     /translation="MSVEHVFVAGAGLMGHGIAQVHAAIGKQVTIYEPDLARAVAGRD
                     RIAGNLERSVAKGRLTAEDRDATVARLRPSDDLATAGDADLVVEAVFEDVEVKSRLWA
                     GLDAIAPAGALFASNTSSISIDALAAAVAQPRRARFVGMHFFSPVPVMPLIELIRGSA
                     TDDATEAAVRALAAELGKQVIVSADRPGFIVNRILMPFLAEAMRAYEEGLGTAEDIDT
                     GARIGLNHPMGPLELADFIGLDVCLGILRVLHEGFEQPQFEPPRVLEQLVADGHLGQK
                     TGRGFHSYPR"
     gene            complement(2082..3278)
                     /locus_tag="AC162A_00315"
     CDS             complement(2082..3278)
                     /locus_tag="AC162A_00315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5009309.1"
                     /translation="MTEIAPGAPLVTRPFVLLTATGLAFFTAGGMVLPAASPYAIEVL
                     GADAIGAGVAVGAFALAALAMRPVVGWASDRFGRRPLLIGGAMLTGVALLAHLAASTL
                     PAFIAVRGAFGIAEAFFFVSVLAAASDLAPPDRRGEALNLVSLSLYLGLAFGPPLGEI
                     ALGAAGYRAVWVLAAALAGVAALLSLTLPETAPAVLSGATERVRARLIHPAAVFPGLL
                     VGCGAFGMAGFLAFLPLYARQVGLDGAALPLAVYALIVVGLRITFAKLPDQVGAARLS
                     GAALAVASIGLVITVVVPTPPGLIVGTAVFAAGVAFIFPALLSLAVSRVDEAERGTAV
                     GTTSVFLDASFGIAPALLGGLAISWGFGGAFLVGAAVAAAGSVTLIARRGSLAAGEPR
                     GVRSGA"
BASE COUNT          489 a         1273 c         1247 g          384 t
ORIGIN      
        1 gcgtgaccgt caggtccgct cgggggctgc atgcggcgga gggtacaccg cgacctcgcg
       61 tcgcggccgc cgccgcgtag catgaccgcc gtgcctgcat cggaccgagg acccgccacg
      121 ccgacgaccc cggaactcgt gctcgcccag cggatgcacg cgctggggtt cctcgccgac
      181 ggcgtcgccc acgacctcgc caacccgctc ggggcgatcc tcgggctggc atcgctgatc
      241 gccgcggacg agcgggtccc cgacgacctg cgcaccgacg cccggctgtt gcgcgaggag
      301 gcggaccgca cctaccgact cgtgtcgacg ctgctcgacg tgacccggac ccggccgccg
      361 gtcctgcggc cgacggccgt cgccccgacg gtgacctcgc tcctcgagct cgcgacccat
      421 ccgctcgcgg gcgtcacggt ggaggtcgcg atcgcgagcg acctgcccga ggtcgaggtc
      481 gacccgtccg cgttccgcta cgcgatcgcg gggctggtgg tcgatgcact agagcgcctc
      541 ggggctccct cggccagcgg ccggctgcgg atcgaggccg ccgcgacggg cgagggcccg
      601 cgggcgaccg tccggctctc gctcattgac gacgggaggc ccgtcgagcc cggcgccacg
      661 accgtgaccg gcatcggcgc gctcctcgat cgcctgggcg tgccggtgat ccaggacacg
      721 atcgacgcgg ccgggcaccc gcgcaaccgg acgctcctcg atctgcgcgt gccgatccaa
      781 ggcgaggcgt ggccggccgc cggggtcgga acgacgccag ggagccctga cgccggcgac
      841 cctcgccgcc cgctcgtcct tgtctgcgac gacgaggcgt cggtccgggc tctgctggtg
      901 cgcgtcctcg agcgcgcggg ctgcgaggcc atcgaggcgg ggagcggcga ggaggcgctg
      961 ctcgcccttg accaagcggc cgtcgacgcg atcgtgtcgg accaccacct gccggcgatg
     1021 acggggatcg acctctacga gcggctcgca gccacccgcc ccgaccttcg acagcggttc
     1081 gtgctcctgt ccggggatcc ggacagcccc gagatcgtgg ccttcatcgc ccgctcgggc
     1141 gtcccgatcg tcgccaagcc gttcgacctc gccgggatcg aggccaccat ccgcgccgtt
     1201 gtgctcccgg tgaccggcgg ctgatcagcg cgggtacgag tggaacccgc gcccggtctt
     1261 ctggcccagg tgaccgtcgg ccaccagctg ctccagcacc cgcggcggct cgaactgcgg
     1321 ctgctcgaag ccctcgtgaa ggacgcgcag gattccgagg cagacgtcga ggccgatgaa
     1381 gtcggcgagc tcgagcggcc ccatcgggtg gtttagcccg atcctcgccc cggtgtcgat
     1441 gtcctcggcg gtgcccagcc cctcctcgta ggcccgcatg gcctcggcga ggaacggcat
     1501 caggattcgg ttgacgatga acccgggccg gtcggccgac acgatgacct gcttgccgag
     1561 ctcggcagcg agggcccgga cggcggcctc ggtcgcgtcg tcggtcgccg agcctcggat
     1621 cagctcgatg agcggcatca ccggtacggg gctgaagaag tgcatgccga cgaacctcgc
     1681 ccggcgcggc tgcgccaccg cggccgcgag ggcgtcgatc gagatcgacg aggtgttgga
     1741 cgcgaacagg gcgcccgcgg gcgcgatggc gtcgagccca gcccacaggc gcgacttcac
     1801 ctcgacgtcc tcgaacaccg cctccacgac gaggtccgcg tcgccggcgg tcgcgaggtc
     1861 gtcgctcggg cggagacggg cgacggtcgc gtcccggtcc tcggcggtca gccggccctt
     1921 ggcgaccgag cgctcgaggt tccccgcgat ccggtcccgg ccggcaacgg cccgggcgag
     1981 atccggctca tagatggtca cctgtttgcc gatcgcggcg tgcacctgtg cgatcccgtg
     2041 gcccatcagc cccgcccccg cgacgaacac gtgctccacg ctcatgcgcc agagcgtacg
     2101 ccgcgcggct cgccggccgc gagcgacccg cgccgcgcga tgagggtgac ggatccggcc
     2161 gcggcgacgg ccgccccgac gaggaacgcg ccgccgaacc cccagctgat cgcgagcccg
     2221 ccgagcagcg ccggcgcgat cccgaacgag gcgtcgagga acacgctcgt cgtgcccacg
     2281 gcggtcccgc gttccgcctc gtcgacccgc gaaacggcga gcgacagcag ggcaggaaag
     2341 atgaacgcca cgcccgcggc gaagaccgcg gtcccgacga taagccccgg cggcgtgggg
     2401 acgacgacgg tgatcacgag gccgatggat gcgaccgcga gggccgcgcc cgagaggcgg
     2461 gccgcgccga cctggtcagg gagcttggcg aacgtgatcc ggagcccgac cacgatcagc
     2521 gcgtagaccg cgagcgggag tgccgctccg tcgagcccca cctggcgggc gtacagcggc
     2581 aggaaggcga ggaacccggc catcccgaat gccccgcagc cgacgagcag gccggggaac
     2641 acggccgccg ggtggatcag gcgcgcccgg acgcgctcgg tggcgcccga gagaaccgcg
     2701 ggagccgtct ccggcagcgt cagggagagc agggcggcca cgcccgcgag ggccgcggcg
     2761 aggacccaga ccgcccggta ccccgccgcc ccgagcgcga tctcgcccag cggcggtccg
     2821 aacgcgaggc cgagatacag cgagagcgag acgaggttga gcgcctcgcc gcggcgatcc
     2881 ggcggcgcga ggtcgctggc ggccgccagg accgacacga agaagaacgc ctcggcgatc
     2941 ccgaacgcgc cgcggaccgc gatgaaggct ggcagcgtcg acgccgcgag atgggcaagc
     3001 agcgcgaccc ccgtcagcat cgcgccgccg atcagcaacg ggcgacgccc gaagcggtcc
     3061 gacgcccagc cgacgacggg gcgcatcgcg agcgccgcca gcgcgaatgc ccccaccgcc
     3121 acgcccgcgc cgatcgcgtc ggcccccagg acctcgatcg cgtagggcga tgcggccggc
     3181 aggaccatgc cgcctgcggt gaagaacgcg aggcccgtgg ccgtcagcag gacgaacggg
     3241 cgcgtgacga gcggcgctcc cggggcgatc tcggtcacgg gcggtcccgc aggccctcga
     3301 gcagctggtc cgcggcggcg taggggtcga gctcgtggct cgcgacggcg tcgattgcgg
     3361 cgtccgtgcc ggcccgtcga tcagcggcac cga
//
LOCUS       DBNHXO010000016         5142 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5162256, whole genome shotgun sequence.
ACCESSION   DBNHXO010000016 DBNHXO010000000
VERSION     DBNHXO010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5162256"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="AC162A_00320"
     CDS             complement(<1..383)
                     /locus_tag="AC162A_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR5009310.1"
                     /translation="MTGAAFDALAVGSDIQRRIGVKTSRDEPLAWFTTMRVGGPADLF
                     AVAHNLFELRGLARYARARSIPLFLLGRGSDLVIADAGIRGLVVQVRAEATSIEGERF
                     LAGAGVPMARAATESQKAGLGGLEYG"
     gene            complement(380..1528)
                     /locus_tag="AC162A_00325"
     CDS             complement(380..1528)
                     /locus_tag="AC162A_00325"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - obsolete lipid glycosylation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIR5009311.1"
                     /translation="MRLLIAGGGTGGHIYPALAVARSLRARPDAAELAWLGGHRGLEA
                     SLVPPAGIALRRLFLRSLRSAGRDIHLVLDPIRLGFSIPQAIVLLIARRPAAIFTTGG
                     YVAIPTLLAATLLRIPSVLWEGNVVPGRSVRFVARLATVLAVSYAETAVALGHERAYV
                     TGTPIRPLSGVDPDEARATFGGRPGERILLVFGGSQAVRRINDAVIAALPRLVERVRV
                     VHVVGDAGYGAALAARQTLPEALRDRYRPYPFLHEEMTAALAAADLAVGRAGASTLAE
                     AAAFALPMAIVPYPHAAGHQRRNAELHAAAGAAILVEDEALDADRLVEVAGLLVDPAS
                     HAAMSAAARDLARPAAAAAVADLVLAAAHREALPSQDEIETVARGVLP"
     gene            complement(1434..2717)
                     /locus_tag="AC162A_00330"
     CDS             complement(1434..2717)
                     /locus_tag="AC162A_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR5009312.1"
                     /translation="MSTTPLTAVRATPKAAQRTKAQITLKRERHEPEYGILVAIVALA
                     AIGILMVYSSSAMKAFLQRDDTFAIVGPQIQWAALGMVAMVAMMRIDYRWLRVASVPL
                     FIIGIVSLDLVFVPQINIVVGGSARWLKIGPLPAVHPAEFMKLALVVYLAHWFAKRGS
                     GVRGLWGGTAPFLVITVPVVVLVLLEPDLGTSSVLAFTAFTLFFLAGANLIHLAGLAA
                     AAVPAVLLMFVRGYQMDRIYAWLDPWKDTTGLGFHSVQGYLALALGGLVGAGLGESRL
                     AGGLFLPNASNDFIFAIIGEEFGLLGAGLVIVLFLAFGYLGIRTSLGAPDTFGALLAA
                     GITGWICIQAFINIAVVVGLIPVTGIPLPFVSAGGSSLIISFAAIGILLSVSRETVEK
                     GTWNDAPADRGRGNGRSHLPGPRRRPVASRATRRG"
     gene            complement(2714..4267)
                     /gene="murD"
                     /locus_tag="AC162A_00335"
     CDS             complement(2714..4267)
                     /gene="murD"
                     /locus_tag="AC162A_00335"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR5009313.1"
                     /translation="MPTPDMTAARPIDLDALRLEDVRGGALADRPVTVLGLARSGIAL
                     ARFFADAGARVTIYDGRQAAELERGIAQLGDRAVTLRLGPDVDPASTWAGAALVATSP
                     SITADYPTTEPRLRGQLQALVARRAAGDADTPALVAEADLFLRLCPAPTVGVTGTKGK
                     TTTSALTHAILAADPLHPAVLGGNIGVPLIERLPELTADHRVVIELSELQLPALSRGT
                     TVAVYTNVTSDHLDRHGSIEAYRRVKRRLAELVDPAGAVVLNLDDPVVAGYPRRDTAA
                     VVGYRDDRPISGGVGVIDEWIVAADVERLALAGGGPAQTGPGGGIMPIDELSIPGRHN
                     VSNALAAVAVGLLFGVGPDAIRGAAAGFTGVEHRLQPVGLRDGVRYVNDSQGTQPDAV
                     IAALRAFEPPVVLIAGGRDKGVDLTELAAVAAETASAAVLIGESGPALRDLLIAAGIA
                     RTAEAGSMDEAVATADALARDALHHQPPGSVATVLLSPAAASFDMFEDYAARGRAFIA
                     AVGRLGATS"
     gene            complement(4269..>5142)
                     /gene="mraY"
                     /locus_tag="AC162A_00340"
     CDS             complement(4269..>5142)
                     /gene="mraY"
                     /locus_tag="AC162A_00340"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR5009314.1"
                     /translation="IRQEGPETHYVKEGTPAMGGVLIVLVVLGIYFFLREPDAATFAP
                     LAALAGVGALGAFDDYLNAKTGEGIRARQKLIWLTVVAFFAAYQIQQTYAITAIAVPF
                     VGPVQIDPWLYILFGAFAIVAAANGVNLTDGLDGLSGGTVAIAFVAYMLIALLNVPNQ
                     ANLALLCALIIGALLGFLWFNVHPAQIFIGDSGALSLGATLAVTALITGQILVLPLIG
                     IIFVVETTSVILQVAYFRATGGKRLFRMSPIHHHFELGGWDEEKITLRFWIVGILSGL
                     LGVTLFLASINALA"
BASE COUNT          800 a         1849 c         1881 g          612 t
ORIGIN      
        1 ccgtactcga gcccacccag cccggccttc tggctctccg tcgcggcgcg ggccatcggc
       61 accccggctc ccgcgaggaa ccgctcgccc tcgatgctcg tcgcctcggc acgcacctgg
      121 acaacgaggc cgcgaatccc ggcatcggcg atgacgaggt cgctccccct gccgagcagg
      181 aacagcggga tcgaacgcgc gcgcgcgtag cgcgcgaggc cgcgcagctc gaacaggttg
      241 tgggcgaccg cgaacaggtc cgccggcccg cccacgcgca tggtcgtgaa ccacgcgagc
      301 ggctcgtcgc gcgacgtctt gacgccgatc cggcgctgga tgtcggaccc gaccgcaagg
      361 gcatcgaacg ccgccccggt cacgggagga ccccgcgcgc gaccgtctcg atctcgtcct
      421 ggctcggcag ggcctcgcgg tgggccgcgg cgagcaccag gtcggcgacg gccgcggccg
      481 cggcgggccg ggcgaggtcg cgcgcggcgg cggacatggc cgcgtggctg gccgggtcga
      541 ccagcaggcc cgcgacctcg accaggcggt cggcatcgag tgcctcgtcc tcgaccagga
      601 tcgccgcgcc cgccgcggcg tgcagctccg cgttccggcg ctggtgcccc gcggcgtgcg
      661 ggtacgggac gatcgccatc ggcagggcaa aggccgccgc ctccgcgaga gtcgaagccc
      721 cggcgcgccc gacggcgaga tcggccgccg caagggccgc ggtcatctcc tcgtgcagga
      781 atggatacgg gcggtagcgg tcgcgcaggg cctcgggcag cgtctggcgc gcggcgaggg
      841 ccgcgccgta gccggcgtcg ccgacgacgt ggaccacgcg cacccgctcc acgagcctcg
      901 gcagggccgc gatcacggcg tcgttgatcc gccgcacggc ctgcgacccg ccgaacacga
      961 gcaggatccg ttcgcccggc cggccgccga acgtcgcgcg tgcctcatcc ggatccacgc
     1021 ccgacagcgg gcggatcggg gtcccggtca cgtaggcgcg ctcgtggccc agggccacgg
     1081 ccgtctccgc gtagctgacc gcgagcaccg tcgcgagccg ggccacgaag cgcacgctgc
     1141 ggccgggcac gacgttcccc tcccacagca cgctcgggat ccgcagcagc gtcgcggcca
     1201 ggagcgtcgg gatcgccacg tagccgccgg tcgtgaagat cgcggccggg cggcgcgcga
     1261 tcagcagcac gatcgcctgg gggatcgaga agccgagccg gatcgggtcg aggacgaggt
     1321 ggatgtctcg cccggccgag cgcagggagc gcaggaacag ccggcgcagc gcgatgcccg
     1381 cgggcgggac cagcgaagcc tcgaggccgc gatggccgcc gagccaggcc agctcagccg
     1441 cgtcgggtcg cgcgcgaagc gaccgggcga cggcgagggc cgggtagatg tgaccgcccg
     1501 ttcccccgcc cgcgatcagc aggcgcatcg ttccaggttc ctttctcgac cgtctcgcgg
     1561 gacacggaca acaggatccc gatggcggcg aagctgatga tcagcgacga cccaccggcc
     1621 gacacgaacg gcaacgggat gccggtcacc gggatcagcc ccaccacgac cgcgatattg
     1681 atgaacgcct ggatgcagat ccagccggtg atgcccgcgg ccagcagggc gccgaacgtg
     1741 tcgggtgccc cgagcgacgt ccggatcccc aggtagccga acgcgaggaa cagcacgatc
     1801 accagccccg cgccaaggag cccgaactcc tcgccgatga tcgcgaagat gaagtcgttg
     1861 gacgcgttgg gcaggaacag gccgcccgcg aggcggctct cgcccaggcc cgcgccgacg
     1921 agcccgccca gggccagcgc gaggtagccc tggacgctgt ggaacccgag cccggtcgtg
     1981 tccttccacg gatccagcca ggcgtagatg cggtccatct ggtacccgcg cacgaacatc
     2041 agcagcaccg ccggcacggc ggccgcggcg agccccgcca ggtggatgag gttggcgccc
     2101 gcgaggaaga acagcgtgaa cgccgtgaac gcgagcaccg acgacgtccc gaggtccggc
     2161 tcgagcagga ccagcaccac gaccgggacc gtgatgacga ggaacggcgc cgtgccgccc
     2221 cacaggccgc ggacgccgct cccccgcttc gcgaaccagt gggcgagata gacgacgagg
     2281 gcgagcttca tgaactcggc cgggtggacc gccggcagcg gcccgatctt gagccagcgg
     2341 gccgacccgc cgacgacgat gttgatctgg ggcacgaaca cgaggtccag gctcacgatc
     2401 ccgatgatga acagcggcac cgaggccacg cgcagccagc ggtagtcgat ccgcatcatc
     2461 gcgaccatcg cgaccatccc cagggccgcc cactggatct gcgggccgac gatcgcgaac
     2521 gtgtcgtcgc gctggaggaa ggccttcatc gcggacgacg agtagaccat caggatgccg
     2581 atggcggcga gggcgacgat cgcgaccagg atcccgtact cgggctcgtg gcgctcgcgc
     2641 ttgagcgtga tctgggcctt cgtccgctgg gccgccttgg gcgtggcgcg gaccgcggtc
     2701 aggggggtcg tgctcatgag gtcgctccca ggcgcccgac ggcggcgatg aaggcccggc
     2761 cgcgggccgc gtagtcctcg aacatgtcga acgacgccgc ggccgggctg agcagcacgg
     2821 tggccacgga tcccggcggc tggtggtgga gcgcatctcg cgcgagcgcg tccgcggtcg
     2881 ccaccgcctc gtccatcgag cccgcttcgg ctgtccgggc gatgcccgcc gcaatgagca
     2941 ggtcccgcag cgcaggcccg ctctccccga tcaggacggc ggccgacgcg gtctccgcgg
     3001 cgaccgccgc gagctccgtc aggtccacgc ccttgtcccg gccaccggcg atgaggacga
     3061 ccggcggctc gaacgcgcgg agggccgcga tcacggcgtc gggctgcgtc ccctgcgagt
     3121 cgttgacgta gcggaccccg tcccgcagcc cgacgggctg cagccggtgc tcgacgccgg
     3181 tgaagccggc cgccgcgccc cgtatggcgt ccggcccgac cccgaacagc agcccgaccg
     3241 ccacggcggc gagggcgttg gacacgttgt gacgacccgg gatcgacagc tcgtcgatgg
     3301 gcatgatccc gcctccgggg ccggtctggg ccggtccgcc gcccgccagc gcaaggcgct
     3361 cgacgtccgc cgccacgatc cactcgtcga tcaccccgac gccgccggag atgggccggt
     3421 cgtcccgata gccgacgacc gcggccgtgt cccgccgggg atagccggcg acgaccgggt
     3481 cgtcgaggtt gaggaccacg gcgcccgccg gatcgacgag ctcggcgaga cgccgcttca
     3541 cccgtcggta ggcctcgatc gacccgtggc ggtcaaggtg gtcggaggtg acgttggtgt
     3601 agaccgcgac ggtcgtgccc cgtgacagcg ccggcagctg gagctccgag agctcgatga
     3661 cgacccggtg atccgcggtg agctcgggca ggcgctcgat cagcgggacg ccgatgttgc
     3721 cgcccaggac cgccggatgg agcgggtccg cggcgaggat cgcgtgggtc agcgccgacg
     3781 tcgtcgtctt gcccttggtg ccggtcacac cgacggtcgg cgcggggcac agccggagga
     3841 acaggtccgc ctccgcgacc agcgccggtg tgtccgcatc gcccgccgcc cgccgggcga
     3901 ccagcgcctg gagctgcccg cgcaggcgcg gttcggtcgt cgggtagtcg gcggtgatgg
     3961 agggagacgt cgcgaccagg gcggccccgg cccaggtgct cgccgggtca acgtccgggc
     4021 ccagccgcag ggtcaccgcg cggtcgccga gctgggcgat gccccgctcg agctccgcgg
     4081 cttgccggcc gtcgtagatc gtcacccggg cgccggcgtc ggcgaagaag cgcgccaggg
     4141 cgatcccgct gcgcgccagc ccgagcaccg tcactgggcg gtccgcgagc gctccgccgc
     4201 gcacgtcctc gaggcgcagg gcgtcgagat cgatcgggcg ggcggccgtc atgtcgggcg
     4261 tcggcatctc aggcgagggc attgatcgag gccaggaaga gcgtcacgcc gagcaggccg
     4321 gaaaggatgc ccacgatcca gaagcggagc gtgatcttct cctcgtccca gccacccagc
     4381 tcgaagtggt ggtggatcgg actcatccgg aagaggcgct tgccgcccgt ggcccggaag
     4441 taggccacct ggaggatcac cgacgtcgtc tcgacgacga agatgatccc gatcagcggg
     4501 agcacgagga tctggccggt gatcagcgcc gtgacggcca gggtcgcccc taacgacaac
     4561 gcccccgaat cgccgatgaa gatctgcgcc ggatgcacgt tgaaccaaag gaagcccagc
     4621 agcgcgccga tgatcagcgc gcagaggagc gcaaggttcg cctggttcgg gacgttgagc
     4681 agggcgatga gcatgtacgc cacgaaggcg atcgccacgg tcccgcccga gaggccgtcc
     4741 aggccgtccg tcaggttcac gccgttggcc gcggccacga tcgcgaacgc cccgaacagg
     4801 atgtacagcc acgggtcgat ctggaccgga ccgacgaacg ggaccgcgat ggcggtgatg
     4861 gcatacgtct gctggatctg gtaggccgcg aagaaggcca cgaccgtcag ccagatgagc
     4921 ttctggcggg cgcggatgcc ttccccggtc tttgcgttga ggtagtcgtc gaatgctccc
     4981 agggccccga caccggccag cgccgcgagt ggggcgaacg tcgccgcgtc cggctcgcgc
     5041 aggaagaagt agatgcccag gacgacgagg acgatgagca ccccacccat cgccggggtg
     5101 ccctccttga cgtagtgggt ctccgggccc tcctggcgga tc
//
LOCUS       DBNHXO010000017         2295 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5361073, whole genome shotgun sequence.
ACCESSION   DBNHXO010000017 DBNHXO010000000
VERSION     DBNHXO010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5361073"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="AC162A_00345"
     CDS             <1..371
                     /locus_tag="AC162A_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009315.1"
                     /translation="SLWARGERVSAAPTAGDGTVTRDVADGWRAILLRRPRWRAEREI
                     RLESATGDWPAAALAELGEPEPGRVVVDLVGVDARPGANRPPALAIERRDGGPGPTLE
                     AALGRLPAWLLIGRRFRRAS"
     gene            536..1390
                     /gene="efeU"
                     /locus_tag="AC162A_00350"
     CDS             536..1390
                     /gene="efeU"
                     /locus_tag="AC162A_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HIR5009316.1"
                     /translation="MLANYLIGLREGLEAALVIGILLAYLVKIERRDLLPAVWLGVAA
                     AVGISIAFGALLTFGPSTLTFEAQEAIGGTLSIIAVALITWMIFWMARMARYIKADLH
                     AKLDGAINAGAGAVFVVAILAVGREGLETALFLWVGAKAAQTTSAGPLIGAALGLATS
                     FVIGWAIYRGALKLNLRTFFAWTGLFLIVIAAGVLAYGIHDLQEAGILPGLNNLAFDV
                     SATIPPGSILGTTLKGIFNFSPATTWLELIAWVAYIVPVTILFVRLVWGSAPPTRTDA
                     PGKVTTTA"
     gene            1457..>2295
                     /gene="efeO"
                     /locus_tag="AC162A_00355"
     CDS             1457..>2295
                     /gene="efeO"
                     /locus_tag="AC162A_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HIR5009317.1"
                     /translation="MSRQIAVPAPRSLVALALVAVPLAAGCVSNSPTGGAIDVESTAT
                     ECKVASGQAPSGNLTFKVKNSGDEVTEFYLLGEDGLRVISEVENIGPGLTRDLVVQAR
                     PGTYFTACKPGMVGDGLRAAFTVTDSGKSIGPAGDVAKQLKDAEAAYVAYVKDQSGAL
                     ISATEAFADSYTKGDDDAARTAYAPTRVHWERIEPVAESFGDLDPLLDAREADLAEGE
                     TWSGWHMIEKDLWPPTPDANGGTAYVPLTTAERAAAAKDLVTYTQQLVDKVNAPDFTF
                     EAF"
BASE COUNT          327 a          852 c          781 g          335 t
ORIGIN      
        1 cctcgctgtg ggcgcgcggc gagcgcgtgt cggcggcgcc gaccgccggc gacggaaccg
       61 tgacccgcga cgtcgccgac ggctggcgag cgatcctgct gcggcgcccg cgctggcggg
      121 ccgagcgcga gatccgcctc gagtcggcga cgggcgattg gcccgccgcg gcgctggccg
      181 agctcggcga gccggagccg ggcagggtcg tggtcgacct ggtcggtgtc gatgcgcggc
      241 cgggcgccaa ccggccgccg gcgctggcga tcgagcggcg cgacggcgga cccggcccga
      301 ccctcgaggc ggcgctcggc aggcttccgg catggctgct gatcggccgg aggttccgcc
      361 gggcgtcgta gggcgggtcg ccttcagggg tccgaatgaa ggcaccctga gtcaaaacga
      421 caagcaggct tgacacttgc ggtcaggcgt ccctacagtt gccccgtcaa ccgaaccgcc
      481 cccgagttct ccccgctcgg cggcacccac gtcgtgtctg gagctgcgcc gcgccatgct
      541 cgccaactac ctgatcggac tgcgtgaggg cctcgaggcg gcgctcgtca tcgggatcct
      601 gctggcatac ctcgtcaaga tcgaacggcg agacctcctg cccgcggtct ggctcggcgt
      661 cgcggccgcg gtcggcatct cgatcgcctt cggggcgctg ctcacgttcg gtcccagcac
      721 gttgaccttc gaggcgcagg aggccatcgg cggcacgctc tcgatcatcg ccgtcgcgct
      781 gatcacctgg atgatcttct ggatggcccg gatggcccgc tacatcaagg cggacctgca
      841 cgcgaagctc gacggcgcga tcaacgccgg cgccggcgcc gtgttcgtcg tcgcgatcct
      901 cgcggtcggc cgtgagggac tcgagaccgc cctgttcctg tgggtcggcg ccaaggcggc
      961 ccagacgacg tcggcggggc cgctgatcgg cgccgccctc ggcctcgcaa cgtcgttcgt
     1021 gatcggctgg gccatctacc gcggcgcgct caagctcaat ctccggacgt tcttcgcctg
     1081 gaccggcctg ttcctgatcg tcatcgccgc gggcgtgctc gcctacggga tccacgacct
     1141 ccaggaggcc gggatcctgc cgggcctcaa caacctcgcc ttcgacgtgt cggccacgat
     1201 cccgccaggg agcatcctcg gtacgaccct caagggcatc ttcaacttct cgccggccac
     1261 gacctggctc gagctgatcg cctgggtcgc atacatcgtg ccggtcacga tcctgttcgt
     1321 ccggctcgtc tggggctcgg ccccgccgac gcgcaccgat gcgcccggca aggtgacgac
     1381 caccgcctga accccggccg gaccccgccg cggtccggcg cccagcccaa ccccactcac
     1441 ccccaccgga ggctcgatgt cccgccagat cgccgtcccc gccccgcgct cgctcgtcgc
     1501 gctcgcgctc gtcgccgtgc cgctggcggc aggctgtgtg tcgaactcgc cgaccggcgg
     1561 cgccatcgac gtggagtcga ccgcgaccga gtgcaaggtg gccagcggcc aggcgccgtc
     1621 gggcaacctc acgttcaagg tcaagaacag cggcgacgag gtcacggagt tctacctcct
     1681 gggcgaggac gggctgcggg tcatcagcga ggtcgagaac atcgggccgg gtctcacccg
     1741 cgatctcgtg gtccaggccc gtcccggcac ctacttcacg gcgtgcaagc cgggcatggt
     1801 cggggatggc cttcgcgcgg cgttcaccgt caccgactcg ggcaagtcga tcggccctgc
     1861 cggcgacgtc gccaagcagc tcaaggacgc cgaggccgca tacgtcgcct acgtcaagga
     1921 ccagtcggga gccctgatct ccgcgaccga ggcgttcgca gactcgtaca ccaagggcga
     1981 cgacgatgca gctcggacgg cctatgcgcc cacccgcgtc cattgggagc gcatcgagcc
     2041 agtcgccgaa tcgttcgggg acctcgatcc gctcctcgac gcccgcgagg cggacctcgc
     2101 cgagggcgag acctggagcg gctggcacat gatcgagaag gacctctggc cgccgacgcc
     2161 cgacgcgaac ggcgggaccg cgtacgtgcc gctcaccacc gccgagcgtg cggcggcggc
     2221 gaaagacctc gtgacctaca cccagcagct cgtcgacaag gtcaacgccc ccgacttcac
     2281 gttcgaggcg ttcca
//
LOCUS       DBNHXO010000018         3219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3178332, whole genome shotgun sequence.
ACCESSION   DBNHXO010000018 DBNHXO010000000
VERSION     DBNHXO010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3178332"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="AC162A_00360"
     CDS             complement(<1..712)
                     /locus_tag="AC162A_00360"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR5009318.1"
                     /translation="MDRFRFITSGESHGPMLGAVVAGVPAGLALTEDDLAVDLARRQR
                     GYGRGARQAIEHDRARILGGVRHGVTLGSPILLEVANRDWENWTRVMQVGPLSEDEAA
                     ELATLADEGNKRAQPITRVRPGHADLAGALKYGFNDVRDVLERSSARETTARVAAGGV
                     ARVFLRELGIEVWSFTAEVGGVAVDPSRATRTRDEADTSPLRCPDPDAETAMIARIDE
                     ARSGGDTVGGVFEVVVHGA"
     gene            complement(735..1655)
                     /gene="aroE"
                     /locus_tag="AC162A_00365"
     CDS             complement(735..1655)
                     /gene="aroE"
                     /locus_tag="AC162A_00365"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR5009319.1"
                     /translation="MTKRVVLIGHPVAHSLSGAMQQAAFDHLGIDASYELWDRAPIDL
                     GDAIVELRTDDFLGANVTIPHKERVVPMVDKLTDEAHATGAVNTLTKEGRKLVGHNTD
                     VPGFKVALDVLVGRQKMPRTAIVLGAGGGARAVVYGLIREGFQRVIVFNRHLHRAEGL
                     VKHFGRSAAHMELRAMPWHESIIEAELAKTKVLVNATSIGLGADESPLPAEILNPELL
                     VLDLIYAKTRLLREAAAAGCATSDGETMLLNQGAAAFNLWTGQPAPLEVMGAALADAR
                     AKGLRSAEGEPAGAPADAEAAAAAADSAAG"
     gene            complement(1652..3025)
                     /gene="aroA"
                     /locus_tag="AC162A_00370"
     CDS             complement(1652..3025)
                     /gene="aroA"
                     /locus_tag="AC162A_00370"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR5009320.1"
                     /translation="MSTVATRPAEAGDPADDVLVVRPAARLRGTPRLPGDKSISHRAL
                     LFALLAQGESRIAGAGDGEDVRSTAGLVAALGARVERVAERDGRVDYRVTSPGGDALH
                     EPEGILDCGNSGTSARLVAGVLAGRALFAVLDGDASLRRRPMGRVVEPLRQMGATFAG
                     RGGATLLPLAITGRAPLSPITYDTPVPSAQVKSAILIAGLAADGETWVTEDVATRDHT
                     ERMLRARGVAVDEGAAPDGRHTVRVAGPSSVAVVDETVPADPSAAAFWLVAAAIHPDA
                     ELRLEGVSTNPTRRAIIDILRRMGADIEEHDRPVVGGADGEPLADLVVRSSELRAVDL
                     SPAEVAAAIDEIPILALAAALAQGTTRFRGGGELRHKESDRIAGITDGLGALGADVRV
                     EGDDIEIHGGRPLVGAVTETHDDHRLAMTFAIAGLLATGETIVRRPGSAAVSYPGFAS
                     ELEGVRA"
     gene            complement(3022..>3219)
                     /locus_tag="AC162A_00375"
     CDS             complement(3022..>3219)
                     /locus_tag="AC162A_00375"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935231.1"
                     /note="catalyzes the formation of
                     3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate from
                     phosphoenolpyruvate and D-erythrose 4-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR5009321.1"
                     /translation="AVGADGIMVEVHPRPDEALSDGEQSLTFELFDQMMTALTAVHGH
                     VKDLHGDLVRADGGVMAARGT"
BASE COUNT          415 a         1194 c         1177 g          433 t
ORIGIN      
        1 gggcaccgtg gaccacgacc tcgaacacgc cgcccacggt gtcgccgccg gagcgcgcct
       61 cgtcgatgcg ggcgatcatc gccgtctcgg cgtcgggatc cgggcatcgc agcggagagg
      121 tgtcggcctc gtcccgggtc cgcgtggccc gggacggatc gacggccacg ccgccgacct
      181 cggcggtgaa cgaccagacc tcgatgccca gctcgcgcag gaacacccgg gccacgccgc
      241 ccgccgcgac gcgtgccgtc gtctcgcggg cggacgaacg ctccaggacg tcgcgcacgt
      301 cgttgaaccc gtacttgagg gcgcctgcga gatccgcgtg gccggggcgc acgcgcgtga
      361 tcggctgggc gcgcttgttg ccctcgtccg cgagcgtcgc cagctcggca gcctcgtcct
      421 cggacaacgg acccacctgc atcacgcggg tccagttctc ccagtcgcgg ttggcgacct
      481 cgagcaggat cggggagccg agggtcacgc cgtgcctgac cccgcccagg atccgggcgc
      541 ggtcgtgctc gatcgcctgc cgggctcccc ggccataccc gcgctggcga cgagccaggt
      601 cgacggcgag gtcgtcttcg gtgagcgcga ggccggcggg cacgcccgcg accacggcgc
      661 ccagcatcgg gccgtggctc tcgccggacg tgatgaaccg gaagcggtcc atggcgcctg
      721 ccctcgtccc ccggtcagcc ggcggcggag tcggccgcgg cagccgcggc ctcggcgtcg
      781 gcgggcgcgc ccgccggctc gccctcggcc gaccggaggc ccttggcacg cgcgtcggca
      841 agcgccgcgc ccatgacctc gagcggcgcc ggctgcccgg tccacaggtt gaacgcggcg
      901 gctccctggt tgagcagcat ggtctcgccg tcggacgtgg cgcaaccggc cgccgcggcc
      961 tcgcgcagca ggcgggtctt cgcgtagatg aggtccagca ccaggagctc cgggttgagg
     1021 atctcggccg gcagcggcga ctcgtcagcc ccgagcccga tcgaggttgc gttcaccaga
     1081 accttggtct tcgccagctc ggcctcgatg atcgactcgt gccagggcat cgcccgcagc
     1141 tccatgtgcg ccgcgctgcg cccgaagtgc ttgaccagcc cttcggcccg atggaggtgg
     1201 cggttgaaca cgatcacccg ctggaagccc tcacggatca ggccgtagac gacggcccgc
     1261 gcgccgccgc cggcacccag cacgatcgcg gtccggggca tcttctggcg cccgacgagg
     1321 acatcgagcg cgaccttgaa cccgggcacg tcggtgttgt ggccgacgag cttgcggccc
     1381 tccttggtca gggtgttgac cgcgccggtc gcgtgcgcct cgtcggtgag cttgtcgacc
     1441 atcgggacga cccgctcctt gtgcgggatc gtgacgttgg cgccgaggaa gtcgtcggtg
     1501 cgcagctcca cgatcgcgtc gccgaggtcg atcggcgcgc gatcccacag ctcgtacgac
     1561 gcgtcgatgc cgaggtggtc gaaggcggcc tgctgcatgg cccccgagag cgaatgggcg
     1621 accgggtggc cgatgaggac gacgcgtttc gtcatgcccg aactccctca agttcgctcg
     1681 caaagcccgg atacgagacc gccgccgacc caggccggcg gacgatcgtc tcgcccgtgg
     1741 cgagcaggcc cgcgatcgcg aacgtcatcg cgagccggtg atcgtcgtgt gtctcggtga
     1801 ccgcgccgac cagcggccgg ccaccatgga tctcgatgtc gtcgccctcg acgcggacgt
     1861 ccgccccgag cgccccgagt ccgtccgtga tcccggcgat ccggtcggac tccttgtgcc
     1921 ggagctcgcc gccgccccgg aaccgcgtcg ttccctgggc gagggccgct gccagcgcca
     1981 ggatcgggat ctcgtcgatg gccgctgcaa cctcggcggg tgacaggtcg acggcccgaa
     2041 gctcgctgga tcgcacgacg aggtcggcga gcggctcgcc gtccgccccg ccgacgacgg
     2101 gacgatcgtg ctcctcgatg tcggcgccca tgcgcctcag gatgtcgatg atggcacgcc
     2161 gcgtcgggtt cgtgctcacc ccttcgagcc gcagctcggc gtcggggtgg atggcggcgg
     2221 caacgagcca gaaggcggcc gccgatggat cggccgggac ggtctcgtcc acgacggcga
     2281 cgctcgatgg ccccgcgacc cggaccgtat ggcgaccgtc cggcgcggcg ccctcgtcca
     2341 cggcgacgcc gcgggctcgc agcatgcgtt cggtgtggtc gcgggtggcg acgtcctcgg
     2401 tcacccacgt ctcgccgtcc gcggcgaggc cggcgatcag gatcgcggac ttgacctgcg
     2461 cgctgggaac gggcgtgtcg taggtgatcg gggacagggg agcgcggccc gtgatcgcaa
     2521 gcgggagcag cgtcgccccc ccgcgaccgg cgaacgtcgc gcccatctgg cgcagaggct
     2581 cgacgacgcg gcccatcggt cgccgccgga gcgacgcgtc gccgtcgagt acggcgaaca
     2641 gcgcacggcc ggcgagcacg ccggcaacga ggcgcgccga cgtcccggag ttgccgcagt
     2701 cgaggatgcc ctcgggctcg tgcagggcgt cgccgcccgg ggaggtcacg cggtagtcga
     2761 cgcgcccgtc gcgctcggcg acccgctcga cccgtgcccc gagagccgcg accagcccgg
     2821 cggtcgagcg cacgtcctcg ccgtcccccg cgccggcgat ccggctctcg ccctgggcca
     2881 gcagcgcgaa cagcagcgct cggtgggaga tggacttgtc gcccggcagc cgcggggtcc
     2941 cgcgcaggcg cgcggccggc cgcacgacga gcacgtcgtc ggcagggtcg ccggcctccg
     3001 ccgggcgcgt cgccacggtg ctcacgtccc tcgagcggcc atgacgccgc cgtcggcgcg
     3061 gacgaggtcg ccgtggaggt ccttgacgtg gccgtggacg gcggtcagcg ccgtcatcat
     3121 ctggtcgaac agctcgaacg tgagcgactg ctcgccgtcg gacagcgcct cgtcggggcg
     3181 cggatggacc tcgaccatga tcccgtcggc cccgacggc
//
LOCUS       DBNHXO010000019         4806 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3178629, whole genome shotgun sequence.
ACCESSION   DBNHXO010000019 DBNHXO010000000
VERSION     DBNHXO010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3178629"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="AC162A_00380"
     CDS             <1..767
                     /locus_tag="AC162A_00380"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5009322.1"
                     /translation="LQGDVWGWTSAAVIGGFVLALVALVAFVLRERSTPEPAIHLDVF
                     RSRAFVASTLVGAAAWFGILSGSVQLAVYLQEVRGLSPTQAALVLLPWPLLAAVLFPR
                     AASIVARIGASKVMVWSLVFALAAAVLMAAFDASTPFVVVSAVAALGGVPIALGVVAS
                     TVTALSEFRPEDAGIAAGAFNSLRQVGSALGVAIPAAAFDYALTPGAADLMPGSHAAF
                     VSRVIVFAIALVAVLLLLRRPLAAPAPVPAAGVGAD"
     gene            790..2436
                     /gene="metG"
                     /locus_tag="AC162A_00385"
     CDS             790..2436
                     /gene="metG"
                     /locus_tag="AC162A_00385"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR5009323.1"
                     /translation="MSDRRRYYLTTAIAYANNAPGLHTVYEVIEADVIARWHRMQGDD
                     TRFLTGTDEHSVNIAQAAEAQGRTPREFVDEKVALFRSAEDALLISPDRFIRTTDPDH
                     YLSAQEMVRRAYDNGDIYLGTYEGWYCPNEGFKATSDLLETARGMQCPNHPDVELQWL
                     TERNWFFRLSAYQERLLDHYEQHPDFVQPDYRRNEMLGFIRGGLEDFSISRAGATWGI
                     PFPIAENGETAQREDGSWDPEAGTIYVWYDALINYITGAGFPGDLDAFHHWWPADLHV
                     IGKDIARFHTIYWPAMLWSAGIDAPRHVWVHGWLLVQGERMSKSRGNFLDPHAVVAAF
                     GADGARYVTLREVAFDRDTDVSWDSFVRRYNADLANDFGNLVNRTVTMVNRYLGGERP
                     APRRAGDSPLGEGWTDTLRVYGERLDACRLHDALAELWEFVGGANKTVDAEQPWTLNK
                     AALAGDAEAAARLRDVLGDLVEACRLVGLAVAPFMPSIAPRVLEQLGHDFPYAADGNG
                     GPPILELLAWGAAGEAGRVTDAPAPLFPRVEVEAAEAPTA"
     gene            2452..3288
                     /locus_tag="AC162A_00390"
     CDS             2452..3288
                     /locus_tag="AC162A_00390"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR5009324.1"
                     /translation="MRLVDSHGHVNADRFADDVELVLGAARLAGVERILVPGWNHASS
                     GRALALVERYPWLDAAVGVHPHDAAKVTDAEWAAIEAWARDERVVAVGETGLDSDRMF
                     SPWDAQLDNLRRNLALALGTGKPAILHCRSKAGARDAQDALVEELRRAGLDEAGARAS
                     FGDRPPAVIHSFSGPVDYAREVLALGLAVSFSGLVFRAGEEASAEVAEIVPAEQLLVE
                     TDAPFLTPRGAARSRNEPAFVALTARWVAERRGVGEEGLGELGDGLVAAYDAVFPRRR
                     QR"
     gene            3327..3731
                     /locus_tag="AC162A_00395"
     CDS             3327..3731
                     /locus_tag="AC162A_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009325.1"
                     /translation="MGALAIASWVAGCGVVDLATPLPPGSRRWVITVENQSDRPAIVE
                     VAEDAGQEGPIVGTVSPNPIPAGATVDVMFGIPPGRGWAIFVNPGPDRGPLITSSDVP
                     PVAQGKLPVKIVIGSDGSPGAQVPSLPGWFGN"
     gene            complement(3745..4767)
                     /locus_tag="AC162A_00400"
     CDS             complement(3745..4767)
                     /locus_tag="AC162A_00400"
                     /inference="COORDINATES: protein motif:HMM:NF016013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HIR5009326.1"
                     /translation="MATDEGALAGSAADDILIDARQSLGAATLADLFPDGPAFRQSVV
                     TFTVLLALAGLIATFGLIQDSIAAIIGAMVVAPLGGAMMALAGALVTGRSRWQLNSFV
                     EVVLGSLLVVAIGFVVAAVLPDPLTLNPSLAARTEPGLLDLGVALAAGAAGAFVTVRR
                     TGSDALPGVAIAVSLVPPLATVGICLELGRTSDAAGALTLFLTNFAAIVVIASIVFAI
                     CGVRPVPAPANAGRVLARGLVLAVIGLALISIPLAVTAAERVRTVVWSNAAAPLVDDW
                     IGDLDLEVTDWGIEGDTVTIDLRGTDQPPPVAPLAASIARAIDHAVEVQVNYVHIERE
                     QATAAP"
BASE COUNT          631 a         1745 c         1752 g          678 t
ORIGIN      
        1 tgctgcaggg cgacgtctgg ggctggacgt cggcggcggt gatcggcggc ttcgtgctcg
       61 cccttgtcgc gctcgtcgcg ttcgtgttgc gggagcgatc gacgcccgag cccgcgatcc
      121 atctcgatgt gtttcgcagc cgcgcgttcg tggcctcgac gctggtcggc gccgctgcct
      181 ggttcgggat cctgtcgggg tcggtccagc tcgccgtgta cctgcaggag gtccgcggcc
      241 tgtcgccgac gcaggccgcg ctcgtgctgc tgccgtggcc gctcctggcc gccgtgctgt
      301 ttccccgggc ggcgtcgatc gtcgcccgca tcggcgcctc gaaggtgatg gtctggagcc
      361 tcgtcttcgc cctcgcggcg gccgtgctga tggcggcgtt cgacgcctcg acgccgttcg
      421 tcgtcgtgtc ggccgtcgcg gcgctgggcg gtgtgccgat cgcgctgggc gtggtcgcga
      481 gcacggtgac cgccctgtcg gagttccggc ccgaggacgc gggcatcgcg gcgggcgcgt
      541 tcaactcgct gcgccaggtc ggctccgcgc tcggcgtcgc gatcccggcg gccgccttcg
      601 actacgcgct cactcccggg gccgcggacc tgatgccggg gtcgcacgcg gcgttcgtga
      661 gccgggtcat cgtgttcgcc atcgccctgg tcgccgtcct gctgctgctc cgccgcccgc
      721 tggccgcgcc cgcgcccgtg ccggccgccg gggtcggcgc cgactgaccc cctgctacga
      781 tcgcgccgca tgtccgatcg ccgccggtac tacctgacca ccgccatcgc gtacgccaac
      841 aacgcccccg gcctgcacac cgtgtacgag gtcatcgagg ccgacgtcat cgcgcgctgg
      901 caccggatgc agggggacga cacccgcttc ctgaccggca ccgacgagca ctcggtgaac
      961 atcgcccagg cggccgaggc gcagggtcgc acgccgcgcg agttcgtcga cgagaaggtc
     1021 gccctgttcc gatcggccga ggatgcgctg ctgatcagcc ccgaccggtt catccggacg
     1081 acggacccgg accactacct gtccgcccag gagatggtcc ggcgggccta cgacaacggc
     1141 gacatctacc tcggcaccta cgagggctgg tactgcccca acgaggggtt caaggcgacg
     1201 tcggacctgc tcgagacggc gcgcgggatg cagtgcccca accaccccga cgtcgagctc
     1261 cagtggctga cggagcgcaa ctggttcttc cgcctctccg cgtaccagga gcggctgctc
     1321 gaccactacg agcagcatcc cgacttcgtc cagcccgact accggcgcaa cgagatgctg
     1381 ggcttcatcc gcggcggcct cgaagacttc tcgatcagcc gcgcgggcgc gacctggggc
     1441 atcccgttcc cgatcgcgga gaacggcgag accgcgcagc gcgaggacgg cagctgggat
     1501 cccgaggccg ggacgatcta cgtctggtac gacgcgctca tcaactacat caccggggcg
     1561 ggctttcccg gggacctcga cgccttccac cactggtggc cggcggacct ccacgtgatc
     1621 ggcaaggaca tcgcccgctt ccacacgatc tactggccgg cgatgctgtg gtcggccggg
     1681 atcgacgccc cgcgccacgt ctgggtccac ggctggctgc tggtccaggg cgagcggatg
     1741 agcaagagcc gcggcaactt cctcgatccc cacgcggtcg tggctgcgtt cggggcggac
     1801 ggcgcacggt acgtgacgct gcgcgaggtg gcgttcgacc gcgacaccga cgtgtcgtgg
     1861 gactcgttcg tgcgccgcta caacgccgac ctcgccaacg acttcggcaa cctcgtcaac
     1921 cgcaccgtca cgatggtcaa ccgctacctc ggcggcgagc ggcccgcgcc ccgacgggct
     1981 ggggactcgc cgctgggcga gggctggacc gacacgctcc gggtgtatgg ggagcggctc
     2041 gacgcgtgcc ggctccacga tgccctcgcg gagctgtggg agttcgtcgg tggtgccaac
     2101 aagaccgtcg acgcggagca gccgtggacg ctcaacaagg cggccctggc gggcgacgcc
     2161 gaggcggccg cgcggctccg cgacgtcctc ggcgacctcg tcgaggcgtg ccggctggtc
     2221 ggcctcgccg tcgcgccatt catgccctcg atcgcccccc gcgtcctcga gcagctgggt
     2281 cacgacttcc cgtacgccgc tgacggcaac ggcgggcccc cgatccttga gctgctcgcc
     2341 tggggcgccg cgggcgaggc cggccgcgtg accgatgccc ccgcgccgct gtttccgcgg
     2401 gtcgaggtcg aggccgccga agccccgacg gcctgaggcg ccgcgccggt cgtgcgcctc
     2461 gtcgactccc atggccacgt caacgcggac cggttcgccg acgacgtcga gctcgtattg
     2521 ggcgcggccc ggctggccgg ggtcgagcgg atcctggtcc ccggctggaa ccacgcctcg
     2581 tcggggcggg cgctcgcgct cgtcgagcgt tacccgtggc tggacgcggc cgtcggggtg
     2641 catccccacg acgcggccaa ggtcaccgac gccgagtggg cggccatcga ggcgtgggca
     2701 cgggacgagc gggtcgtggc cgtcggcgag accggcctcg actcggaccg catgttctcg
     2761 ccctgggacg cccagctcga caacctgcgc cgcaacctcg cgctggcgct cgggacgggc
     2821 aagcccgcca tcctccactg ccggtcgaag gcgggcgccc gcgacgcgca ggacgcgctc
     2881 gtcgaggagc tgcggcgggc gggtctcgac gaggcggggg cgcgggcgtc gttcggcgac
     2941 cggccgcccg ctgtcatcca cagcttctcg ggtccggtgg actacgctcg agaggtgctc
     3001 gcgctgggcc tcgcggtgag cttcagcggc ttggtcttcc gagccgggga ggaggcctcg
     3061 gcagaggtgg ccgagatcgt cccggcggaa cagctgctgg tcgagaccga tgcgccgttc
     3121 ctgacgcccc gcggcgctgc gcggtcgcgc aacgagccgg cgttcgtggc actcaccgca
     3181 cgctgggtcg cggaacggcg cggggtgggg gaggagggcc tgggcgagct cggggacggc
     3241 ctcgtcgcgg cctacgacgc ggtcttcccc cggcgtcgcc agcgatgacg tggccgcccg
     3301 cacgcaccgc cgcggtcctt cggatcgtgg gcgcgctcgc gatcgcatcc tgggtcgcgg
     3361 gctgtggcgt cgtcgacctc gcgaccccgc tcccacccgg ctcgcgccgg tgggtgatca
     3421 cggtcgaaaa tcagagcgac cggccggcga tcgtcgaggt cgccgaggat gcgggtcagg
     3481 aagggccgat cgtcggcacg gtcagcccga acccgatccc ggccggcgcg acggtcgacg
     3541 tcatgttcgg gatcccgccc ggccgcgggt gggcgatctt cgtcaatcca ggcccggacc
     3601 ggggcccgct gatcacgtcg tccgacgtgc caccagtcgc ccagggcaag ctccccgtca
     3661 agatcgtcat cggcagcgat ggaagtcccg gcgcccaggt cccgagcttg ccgggctggt
     3721 tcggcaactg accccggcgc gacgtcacgg cgcggcggtc gcctgctcgc gctcgatgtg
     3781 cacgtagttg acctggacct cgaccgcgtg atcgatcgcc cgggcgatcg aggcggcgag
     3841 gggcgccacg ggcggtggtt ggtcggttcc ccggaggtcg atcgtcaccg tgtcgccctc
     3901 gatgccccag tcggtgacct cgaggtcgag atcgccgatc cagtcgtcca cgagcggcgc
     3961 ggccgcgttg ctccagacga ccgtccggac gcgctcggct gcggtgacgg cgagggggat
     4021 cgagatcagc gccaggccga tcaccgcgag gaccaggccg cgcgcgagca cccggcccgc
     4081 attggcgggt gccggcaccg ggcggacgcc gcagatcgcg aacacgatcg aggcgatcac
     4141 gacgatggcg gcgaagttgg tcaggaacag cgtgagggca cccgccgcgt cgctggtccg
     4201 ccccagctcg aggcagatcc cgacggtggc cagcggcggg acgagggaca cggcgatcgc
     4261 gaccccgggc aaggcatcgc tgccggtcct gcggacggtg acgaaggccc cggcggcgcc
     4321 ggccgcgagg gcgacgccga ggtcgaggag gcccggctca gtgcgcgcgg cgagcgacgg
     4381 attgagggtg agcgggtccg ggagcaccgc cgcgacgacg aacccgatcg ccaccaccag
     4441 cagcgagccg agcacgacct cgacgaacga gttcagctgc caccggctcc ggccggtgac
     4501 gagcgctccc gcgagggcca tcatcgcgcc gcccagcggc gcgaccacca tcgcgccgat
     4561 gatcgcggcg atcgagtcct ggatcaggcc gaaggtcgcg atcagcccgg cgagggccag
     4621 cagcaccgtg aacgtgacca ccgactgccg gaacgcgggc ccgtcaggaa acaggtcggc
     4681 gagcgtggcc gcaccgagtg actgccgtgc atcgatgagg atgtcgtcgg ccgcgctccc
     4741 ggcaagcgct ccctcgtccg tcgccatgct gtctccacgc ggtcgtcctg ccgccgaact
     4801 ctagcg
//
LOCUS       DBNHXO010000020         2362 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2980366, whole genome shotgun sequence.
ACCESSION   DBNHXO010000020 DBNHXO010000000
VERSION     DBNHXO010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2980366"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="AC162A_00405"
     CDS             complement(<1..432)
                     /locus_tag="AC162A_00405"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5009327.1"
                     /translation="MQIIGRGLIAQSLEPYAALHDGTVAFATGVAASTSEDGDGYRRE
                     LDQLRAVLADAAASGERVVYFSGGGAVYGDWSHPVREDDPPRPQTTYGRHQLACERLL
                     AASAVPHLILRLPNVIGPSRNREQLLPSIVAQVLSGTVIVQS"
     gene            complement(432..2153)
                     /locus_tag="AC162A_00410"
     CDS             complement(432..2153)
                     /locus_tag="AC162A_00410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5009328.1"
                     /translation="MSSPTSAGLPPDLRRRIIGSPLVRRRLVEFVVPEAPVEQELRAS
                     LGRVPANARRYAVVGCGIGSLAWELASRHGTAVVVGFDPDATSIDVAGILFERPNLSF
                     VAGPGDAGALDGFDVWVVAPDATLATATGDPLTPGGSRVVTLAAPGRRGPRSGRELWT
                     DATRSDRVTARLGIRVTADGYLVARRPGPAVLIVMPERGGYSETFIRAQIERLPANVS
                     LLVGSLPNMIDEDGAELVPWVGRAGRGALGGLFGGRATQWLDQFWLRRHLSARHIDVV
                     LAEYGTRGIQMISASRQTGIPLVTHFYGYDGTARTIFQKYERGYRRLFRHGGPLVVVS
                     RHMAARLTSIGADPATLSVIACGVDPQPEAVVPAFEDPPVFLAVARFVEKKAPHMTLL
                     AFAALVRDVPEARLTMVGDGPLLGPCKQLARGLGIESCVEFPGAMPHDRLPDLYRSSR
                     AFVQHSMEASNGDTEGLPVAILEASAAGLPVVSTLHAGIPEAVIDGETGYLVPEGGID
                     AMADRLRTLANDRALAQRLGSAARRHVNANFALDDTLGRLEDVLESSIARSARGASRR
                     PGRRRRR"
BASE COUNT          359 a          857 c          842 g          304 t
ORIGIN      
        1 cgactggacg atgaccgtcc ctgacagcac ctgggcaacg atggacggca gcagctgctc
       61 gcggttcctg ctcggaccga tgacgttggg cagccgcagg atgaggtgag gcaccgcgct
      121 cgcggccagc aggcgctcgc aggcgagctg gtggcggcca taggtggtct gcggccgtgg
      181 cggatcatcc tcgcggacag gatggctcca gtcgccatac acggcgcccc cgcccgagaa
      241 gtagacgacg cgttcgccgc ttgccgcggc atccgcgagc acggcgcgga gctggtccag
      301 ctcgcgtcga tacccgtcgc cgtcctcgct ggtcgatgca gcgacgccgg tggcgaatgc
      361 gaccgtcccg tcatgcaggg cggcgtacgg ctcgaggctc tgggcgatca acccgcggcc
      421 gatgatctgc atcatcggcg gcgccggcgg cccggccggc gggacgcgcc gcgagctgat
      481 cgggcgatcg acgactcgag cacgtcctcc agccgcccca gcgtgtcgtc gagggcgaag
      541 ttggcattga cgtgccgcct ggcagccgat cccagccgtt gcgccaacgc ccgatcgttc
      601 gccagcgtcc gcagcctgtc ggccatcgcg tcgatcccgc cttcggggac gaggtatccc
      661 gtctcgccgt cgatgaccgc ctccggaatc cccgcgtgca gggtcgagac cacgggcagg
      721 ccggccgcgc ttgcctcgag gatcgcgacg ggcagccctt ccgtgtcccc gttcgacgcc
      781 tccatcgagt gctgcacgaa cgcgcggctt gaccgataca ggtcaggcag ccggtcgtgc
      841 ggcatggcgc ccgggaactc cacgcaggac tcgatcccca ggcccctggc gagctgcttg
      901 cacggcccga ggagggggcc gtcgccgacc atcgtcaacc gcgcctccgg gacgtcgcgg
      961 accagggcgg caaaggcaag cagcgtcatg tggggcgcct tcttctcgac gaacctggcc
     1021 accgcgagga agaccggggg gtcttcgaag gcgggcacga ccgcctcggg ctgggggtcg
     1081 acaccacacg caatcacgct gagcgtggcg ggatctgccc cgatcgaggt cagccgggcg
     1141 gccatgtggc ggctcacgac gaccagcgga ccaccgtgtc ggaacagccg acggtagccc
     1201 cgctcgtact tctggaagat ggtccgcgcg gtcccgtcgt acccgtagaa gtgggtgacg
     1261 agggggatgc cggtctgccg cgaagcggag atcatctgga tcccccgggt cccgtactcg
     1321 gccagcacga cgtcgatgtg acgcgccgag aggtgtcgcc tgagccagaa ctggtcgagc
     1381 cactgcgtcg cgcggcctcc gaacaacccg ccgagcgcgc cacggccggc ccggccgacc
     1441 cagggcacca gctccgcgcc gtcctcgtcg atcatgttcg gcaggctgcc aacgagcagc
     1501 gacacgttgg ccggcaggcg ctcgatctgc gcccggatga acgtctcgga gtagccccct
     1561 cgctccggca tcacgatcag aacggctggc cctggccggc gggcaacgag gtagccgtcc
     1621 gccgtcactc ggatccccag gcgtgcggtc acccggtccg accgcgtcgc gtccgtccac
     1681 agctcgcgac ccgagcgggg gccccgacgg ccgggagccg cgagggtcac gacgcggctg
     1741 ccgcctggcg tcagcggatc acccgtcgcg gtggccagcg tggcatcggg agccacgacc
     1801 cagacgtcga agccatccag ggcgccggcg tcgccggggc cggcgacgaa cgacaggttg
     1861 gggcgctcga acaggatccc ggccacgtcg atcgacgtgg cgtccggatc gaaccccacc
     1921 accacggccg tcccgtggcg tgacgccagc tcccacgcca gcgatccgat gccgcacccc
     1981 acgacggcgt acctccgggc gttcgccgga acgcggccga gcgacgcgcg aagctcctgc
     2041 tcgaccggtg cctcggggac gacgaactcg acgagccgcc tgcggacgag gggcgacccg
     2101 atgatccggc ggcggagatc gggcgggagg ccggcgctcg tcgggctcga catcagggcg
     2161 gcgagggcag caaggctggc aagcgcggcc gtcgacccgc caggccgcgt gcgcgccagc
     2221 cgacgccgcc tggcgcatcc ggcatgcgtc gtccagcccc gtgggaacgg gtgaacatcg
     2281 gcgtgaacgg taccatcacc cttcgcccga cggcgctgcc gccgacgcgg tatccaggat
     2341 gaggtcggct ccctcagtgg cg
//
LOCUS       DBNHXO010000021        14414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4369656, whole genome shotgun sequence.
ACCESSION   DBNHXO010000021 DBNHXO010000000
VERSION     DBNHXO010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 14414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14414
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4369656"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="AC162A_00415"
     CDS             <1..312
                     /locus_tag="AC162A_00415"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5009329.1"
                     /translation="GMGDGRTSIGGLEVEVRGGRCTLVGSETLAGSVIALDTAVRNLV
                     REGTSLVAAVAAASSNPAALLGATDRGAIEVGRRAHLVELDDDLVVRRVNRGSGWVDP
                     R"
     gene            415..1020
                     /locus_tag="AC162A_00420"
     CDS             415..1020
                     /locus_tag="AC162A_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009330.1"
                     /translation="MAGGWDENASDYSLLAYTSGGALAQIELVGPHLRVGGTIDIGRF
                     SRLSDRVNHSRGYIRIQDARLLKRNGDPTPLLVPVLYVNQDEVTFIAMAHVSDEGSPQ
                     ATAASQDRPLIEKQPRRYLVFTPGHVISGLAHVYRDMTVEGFADTPDPRFIPMTEVSA
                     RSLADRRVVSHFDLLLVNRTQMTAVAEASPDSPADVPVVES"
     gene            1038..1958
                     /locus_tag="AC162A_00425"
     CDS             1038..1958
                     /locus_tag="AC162A_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009331.1"
                     /translation="MVDWFRRAIARAPTPTAPSEPLGDEAPDDPDTEAVEPVTAGAEV
                     IEELEGVSCPSCGTWLIPPPRSNRRCPQCREPIVVRRTEGRTVYFTEAAVAVFEAERQ
                     READELRWTIERREWLRLARVAGAPEARRRRIDGRPPSAEAVGAAKALYLGAADAAVK
                     EARRTKHWTDVGRLRREEAAALFAEAGHPVPPPDDIVALHREGAAAQLRAHAATGTHA
                     ELIGASCCKACRKDDGRAFKIADELRTPRLPHEDCPRGLCSCEWWMSVVAPPRRRRRR
                     SATPAPSPAAIAPPSAADAPSPAADEPAPG"
     gene            complement(1971..2789)
                     /locus_tag="AC162A_00430"
     CDS             complement(1971..2789)
                     /locus_tag="AC162A_00430"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5009332.1"
                     /translation="MGPRDGRFAGRSLLVTGSTGLAASAVRAIAAEGGAVFVTSRTPD
                     HLEALAAEVRGAGGRCEWHAADLRVEADVDAVFAAFDATFGRLDGVYSVAGISARRFG
                     DGPVHEATLEGWESVIAANATSQFLVARAAVRRMLAQAPDAAGSRGSVLLMSSLLATR
                     PVPEHFATHGYAASKAAIEGLTRAMAATYAPDAIRVNAIAPSVVATPMSRRAQEDETV
                     SAYLAAKQPLAGGPLDADAVTAIALALLGDEGRMVTGQVIAVDGGWSVAEGPRP"
     gene            complement(2789..3442)
                     /locus_tag="AC162A_00435"
     CDS             complement(2789..3442)
                     /locus_tag="AC162A_00435"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF013263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - (R,S)-4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIR5009333.1"
                     /translation="MKADDLRAGPIAAAIREHRLVVVLRRVTPLAALLEMVDELADAG
                     ARVFEVTFDASSAEADLVAVRERLGRRADGPFLTGAGTVLERSQLEAARRARADFAVA
                     PTLDLALVGTAVAEGMPFIPGALTPTEIGSAWAAGATFVKLFPASAVGPTFVRELRGP
                     MPAVQLIATGGVDAANAPAFLQAGAAAVGVGGAITRAGADVRRAIVAAVAGVGVEGR"
     gene            complement(3439..4743)
                     /locus_tag="AC162A_00440"
     CDS             complement(3439..4743)
                     /locus_tag="AC162A_00440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5009334.1"
                     /translation="MTDRYDVVVVGSGSAGSSAAITAARHGARTLLLDRLAFMGGTST
                     AVLDTFYAFYTPGTHPRRVVGGLGWEVASALTANGVAFERPNTYGAGTGVTYDAEALK
                     VLWERLAEDAGVELLLHTWATGVRIDGDRRLRAVRLWNKGGERWVEAAAFVDASGDAD
                     VCAQAGAGYDDASSTPGGVQSLSTLFKLANVDIERATAVPKAELWALMRDAVEHGGYR
                     LPRVEGSWHRTPYPGVVLIHMTRIPNVDATDPAQLTRAEIEGRRQVQEYHRFLRDRVP
                     GFERSVVVATSPAIGVRESRRVHGDYRLTREDVLGGRRFDDEIALCGAPIEDHGTGGD
                     TAWHYVGEPGVYGIPYRCLLPAAIEGLLMAGRCFSATHDAHASARSMATCMAMGQAAG
                     TAAAMAAAGETTPRALDASRIRERLLDDGALLEPVATGASVG"
     gene            complement(4740..5699)
                     /locus_tag="AC162A_00445"
     CDS             complement(4740..5699)
                     /locus_tag="AC162A_00445"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase family protein"
                     /protein_id="HIR5009335.1"
                     /translation="MPEAHVVSVSGPRPVGVLGVVDAHDHLFLDSPAMPGQAFTDPDR
                     SIAEALEGKATGIGTIVEMTPIGLGRRPAGMRAVADATGLTIIAASGYHRDTHYPDGH
                     WVHAATVETLADRIVTDLTTGMHPSDWDDPGLPLDPARAGAIKGGASYHRITRSEQRR
                     LEAIAGAASATGAAVLVHTEIGTSGHDVVDLLESAGLATDRIALAHMDRNPDAELHVE
                     IAARGVTLEYDTIGRTKYHPDSVVLDLIEALVTNNMTDRIVLGLDLGARDYLRAYDGG
                     PGMRYLMRTFVPRLRRRVGDDVTDRLLVRNPARLYAIAGAGPS"
     gene            5916..6932
                     /locus_tag="AC162A_00450"
     CDS             5916..6932
                     /locus_tag="AC162A_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009336.1"
                     /translation="MARRPRGRLGRFTLRWAAPFIGVLLAYAVVAPGAATAATTWSRN
                     LYVNRAFMYQDPYPTACTAAAAMMMLNTIAYRETGGDGFTWTPTRIKADAGNQRDMLS
                     ILAYARSHDTLRAKGRGSDPHGWRNVLNAFGWGEDAMRDPAKRVYDDRAYRTFDKAVK
                     SAVRAIARRSMPVGVLGWAGGHAQVMTGYVVTGADPRVSNDFVVRYVYLSDPLAGNST
                     VNRRLSLAKFRSGPLRTRFQAYREADSPYDDPWTPGNIKSSVVPSRGQSEWYHRWVIL
                     RPTTPGGQTPDPTPDPDPTASPDPTPTPDQGGEPDPTATPDSTEAPEPTPDEAAPAEE
                     PADS"
     gene            7013..7492
                     /locus_tag="AC162A_00455"
     CDS             7013..7492
                     /locus_tag="AC162A_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009337.1"
                     /translation="MRSYLIVANRTLASPTLAAAVAQRVAEGEATFHVVVPRTPVQHG
                     LTWDEDEAEAAAQQRLDDVLARLKSMGVAATGEVGSKDPVDAVHDALRGREADEIILS
                     TLPVRASRWLGQDVPTKLRRSVNVPVEVVIAPDEGAPAAAGSGDQAADDQAHRVADR"
     gene            7513..10548
                     /gene="pgmB"
                     /locus_tag="AC162A_00460"
     CDS             7513..10548
                     /gene="pgmB"
                     /locus_tag="AC162A_00460"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HIR5009338.1"
                     /translation="MTEGPIYPVDPWRIRELTFDPALAPRNETIFALGNGHLGMRGNL
                     DEDAGNADHGTYVNGFYEEAPIVYPEGAYGYARNHQVLLNVADGKRIALFVGDDAFDL
                     TTGTIEAYERSLDLRDGILTRTVRWRSPGGATVDVVARRLVSLARPAVGAIDFAVTLV
                     EGDARIRIVSAINARVRNQEASDDPRVGAHLPANVLETIHHEASGTFGAVVQRTQATR
                     LAIVAAADHVVRSDGASLDGQSSTADEGGFALTLDASLAPGGSLGVTKLLAYCTSRDH
                     PEEALLDRARDAIGLARETGFDGLAAEQRAVLDGFWSASDVEIDGDEMLQQGVRFNLF
                     SLFQSAGRDGRTSLAAKGLSGEGYEGHYFWDTEIFALPFFGYTQPGIARALLRYRCGI
                     LDKARARAAEMSQRGALYPWRTIGGEEASAYFPAGTAQYHINADIAYAIEKYVAASGD
                     RSLLLEGGAEVVFETARLWADLGDYIPARDGAFCINEVTGPDEYTALVNNNGYTNLMA
                     RAHLRFAARLVDELAADEPAAYDLLAARIGLEPAEVAEWRRAADLMRIPHDAALGVHA
                     QDDSFLDRAPWPFATTPASEYPLLLHYHPLVIYRHQVLKQADVVLAQVLLGSQFTTAE
                     KKRNFDYYDPLTTGDSSLSPCVQSIAAAELGYADAAYRYFMRTARMDLDDVNGNVAHG
                     VHVAAMAGSWMSLIYGFAGLRDDDGSIAFSPRLPDAWDRLAFRLAVGGATLAVTITRH
                     EISYALVGGDALAIRHFGRPVELTAAAPVVIGLDPNLRAVIFDLDGVITDTAEHHYRA
                     WKRLAAEESIPFDRELNERLKGVSRMESLDIILEGAGQTASLADRVRLADRKNAYFRE
                     LIDAITPADLLPGIGDLLADLRARGVRSAIASMSHNVYDVVRHLGIEAMVDVIVDPAT
                     LVKGKPDPEIFLAAADVLGMRYEDCVGIEDARAGIAAIKAARMVAVGVGDDLPGCDWL
                     ISDTRPLTVDALAGLFRRAEAAGRATDPAERIVSADPVA"
     gene            10690..10989
                     /locus_tag="AC162A_00465"
     CDS             10690..10989
                     /locus_tag="AC162A_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009339.1"
                     /translation="MGSLHVALLDGFEDDTVEVLVADQVVARRSGVTTMTQISRADEF
                     DVQLGGATTLVVRLPERGLQATVDLGGVGSATNLGVAVTGDAIQTRISAEPFRFA"
     gene            11061..13316
                     /locus_tag="AC162A_00470"
     CDS             11061..13316
                     /locus_tag="AC162A_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009340.1"
                     /translation="MAIKVEPQPETAALTGSEEEAFGIPFWPPFRLAASGLYTWRFPI
                     SIIPRQPIPLPKLPPIPDPGPWRGGDVTEAGDGQAVDTATSALFPILSERIRLDVDGR
                     YPQMVVSGDMSTGLFSTVHWIANLTKTGSNTYVGAIWYRDPPVAPFPYDSVKVTTGGG
                     WLFDPRTATVTFSGPGGIQRTRTYTYTSPYFHDVDLEVDFQTGVTPATSFATCSHPNR
                     PATLPCETLTIGKVFQRAGFNVTSSPSGTVPVAGAGANATWSNQEMHDAMQVYWSHYA
                     PKAQWATWIFFAATHDMGHGLGGIMFDDIGPQQRQGTSIFIDSFISDPAPAGDPAPAA
                     FIDRMRFWTGVHETGHAFNLAHAWQKSLTSGGKGPWLPLVDEPESRSFMNYPYFVAGG
                     TTAFFADFEYRFSDQELLFMRHAPSSFVQQGNALWFDHHGFLGANALPEPTYQLVVRA
                     NRDRPSFEFMEPVTLELKLTNATAQPQIVDEHILDVDQGLVVIITKKGEPTRQLLPIA
                     RLCRQASQKVLQPAESVYAPMLASVGPNGWNISDPGTYGIQVCLHLPTGEDILSNELR
                     VIVEPARTTEDETLAQDYFTEDVGRVIALEGSRVLDTATDTLTEIADKLRHRRVAVHA
                     NLVLGVPLATEYKVLSRNRKHDGQQYRIEALPARPEEAKDRLVSALSGDPTRSAESFG
                     HVRYKESVDQLTGFLESAGDAADAYKQQDIMLETLSGREVRGRKVLDSVLKEIKTKRD
                     SLASAAGVKSR"
     gene            complement(13409..13681)
                     /locus_tag="AC162A_00475"
     CDS             complement(13409..13681)
                     /locus_tag="AC162A_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR5009341.1"
                     /translation="MSQVRIPPVLRTSAGGQKLIEVEGATVGEALGALVAAYPDLGPR
                     LLDASGGINRFVNVFVNETDVRHLQDLATPLGPGDTVLLLPAMAGG"
     gene            complement(13678..>14414)
                     /locus_tag="AC162A_00480"
     CDS             complement(13678..>14414)
                     /locus_tag="AC162A_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR5009342.1"
                     /translation="VRIDGTYDDVNRLCLEVADELPWGLVNVNLRPFYAEGSKTLGYE
                     IAEELGWRLPDVIVAPIASGAMVTRLARSFEELVELGWVAAKPVRFVGGQAAGCAPVA
                     TAFAAGRSDIEPVRTPDTIVRSLAIGNPADGRYAVELARASEGSIEAITDEATADAIR
                     RTATLEGVYTETAGGVTIAAAEAARRNGTIRDGDEVVALLTGNGLKTPDAVRFGIEDR
                     PAEPGRPGLTPVIPARFAAFEDWLSA"
BASE COUNT         2058 a         5324 c         5100 g         1932 t
ORIGIN      
        1 ggcatgggcg acggtcggac ctcgatcggc ggtctcgagg tcgaggtccg cggcgggcgc
       61 tgcacgctcg tcggcagcga gaccctggcg gggtcggtca tcgcgctcga cacggcggtc
      121 cgcaacctcg tccgagaggg gacgtcgctg gtcgccgccg tggcggccgc gtcgtccaac
      181 ccggcggcgc tgctgggcgc cacggatcgc ggcgcgatcg aggtcgggcg gcgtgcccac
      241 ctggtcgagc tcgacgacga cctggtggtc aggcgggtca accgcggctc cggctgggtc
      301 gacccgcgct gacgggtggc gccggggcgg gcggcgcccg agcgtacaat ccatcgaccg
      361 gcgccccgtc tgacggcgcc tcaccggacc gggtacgacc aggaggcggc gcgagtggcg
      421 ggtggctggg acgagaacgc atcggactac agcctcctcg cctatacctc gggcggcgcg
      481 ctcgcccaga tcgagctcgt cggaccgcac ctccgggtcg gcggcacgat cgacatcggc
      541 cgcttctcga ggctctccga ccgcgtcaac cacagccgcg gctacatccg gatccaggac
      601 gcccggctgc tcaagcgcaa cggcgacccg acgccgctcc tcgtcccggt cctgtacgtg
      661 aaccaggacg aggtcacgtt catcgcgatg gcccacgtct ccgacgaggg gtctccccag
      721 gccacggcgg cgtcgcagga ccggccgctg atcgagaagc agccccggcg gtatctcgtg
      781 ttcacgcccg ggcacgtgat ctcggggctc gcccacgtct accgcgacat gacggtcgag
      841 ggcttcgccg acacgcccga cccgcggttc atcccgatga cggaggtgtc ggcgcgatcg
      901 ctggccgacc ggcgggtggt cagccacttc gatctgctgc tcgtcaaccg gacccagatg
      961 accgcggtcg ccgaggcttc gcccgacagc ccggccgacg tcccggtcgt ggagtcctag
     1021 gccgggggta ggcacgcatg gtcgactggt tccggcgcgc gatcgcccgg gcgccgacgc
     1081 caaccgcgcc gtccgagccg ctcggggacg aggcgccgga cgatccggac acggaggcgg
     1141 tcgagccggt cacggccggt gccgaggtga tcgaggagct cgagggcgtc tcgtgtccgt
     1201 cgtgcgggac gtggctgatc ccgccgcccc gctccaaccg gcgctgcccg cagtgccgcg
     1261 agcccatcgt cgtgcggcgc accgagggcc ggacggtcta cttcacggag gccgcggtcg
     1321 ccgtgttcga ggccgagcgc cagcgcgagg cggacgagct gcgctggacg atcgagcggc
     1381 gcgagtggct gcgtctcgcg cgcgtcgcgg gcgcgccgga ggctcggcgc aggcgcatcg
     1441 acgggcggcc gccgtccgcc gaggcggttg gcgccgccaa ggcgctctac ctcggcgcgg
     1501 cggatgccgc cgtcaaggaa gcccgccgga cgaagcactg gacggacgtc ggacggctgc
     1561 gccgcgagga ggccgcggcc ctgttcgcgg aggcgggcca tccggtgccg ccgcccgacg
     1621 acatcgtggc gctccaccgg gagggagcgg ccgcccagct gcgggcgcac gcggcgaccg
     1681 ggacccacgc cgagctgatc ggcgccagct gctgcaaggc ttgccgcaag gacgacggcc
     1741 gggcgttcaa gatcgccgat gagctgcgga cgccacgact cccccacgag gactgcccgc
     1801 gcgggctgtg cagctgcgag tggtggatgt cggtggtcgc accgccgcgc cggcgtcggc
     1861 ggaggtccgc gacgcccgcg ccgtcgcccg cagccatcgc tccgccgtcc gctgccgacg
     1921 cgccgtcgcc cgctgccgac gagccggccc ccggctgagc cggcggtttc ctagggccgc
     1981 gggccctcgg cgacgctcca gccgccgtcg acggcgatga cctggcccgt gaccatgcgc
     2041 ccctcgtcac cgaggagcgc gagggcgatc gcggtcacgg cgtcggcgtc cagcgggccg
     2101 cccgccagcg gctgtttcgc ggccaggtag gccgacaccg tctcgtcctc ctgggcgcgg
     2161 cggctcatcg gcgtcgcgac caccgacggc gcgatcgcgt tgacgcggat cgcatcgggc
     2221 gcgtaggtgg cggccatcgc ccgcgtgagc ccctcgatcg ccgccttgct cgccgcgtag
     2281 ccatgggtgg cgaagtgctc cgggaccggc cgcgtcgcga gcaggctcga catcagcagg
     2341 acgctcccgc gcgaccccgc ggcgtccggc gcctgggcga gcatccggcg gacggccgcg
     2401 cgcgccacca ggaactggct cgtggcgttc gcggcgatca cggactccca gccctcgagc
     2461 gtcgcctcgt ggaccggtcc gtcgccgaac cgccgtgcgg agatgccggc cacgctgtag
     2521 acgccgtcga ggcgcccgaa cgttgcgtcg aaggcggcga acacggcgtc cacgtccgcc
     2581 tcgacccgca ggtccgccgc gtgccactcg caccggccgc ccgcgccccg gacctcggcg
     2641 gcgagcgcct cgaggtggtc gggagtccgg gaggtcacga acacggcgcc gccctcggcg
     2701 gcaatcgcgc ggacggctga cgccgcgagc cccgtggacc cggtgacgag caggctgcgg
     2761 cccgcgaacc ggccgtcgcg cggccccatc agcggccctc cacgccgacc ccggccaccg
     2821 ccgcgacgat ggcccgacgc acgtcggcac cggcgcgcgt gatcgcgccc ccgaccccca
     2881 ccgcggccgc gcccgcctgg aggaacgccg gcgcgttggc ggcatcgacg ccgcccgtcg
     2941 cgatgagctg gacggctggc atcggcccgc ggagctcgcg cacgaaggtc gggccgaccg
     3001 cggacgccgg aaacagcttc acgaacgtcg cgccggccgc ccaggccgac ccgatctcgg
     3061 tcggcgtcag cgcgcccggg atgaacggca tcccctcggc caccgcagtg cccacgagcg
     3121 cgaggtccag cgtcggcgcg acggcgaagt cggcccgggc gcgccgggcc gcctcgagct
     3181 ggcttcgctc cagcaccgtg cccgcgccgg tgaggaatgg gccgtcggct cgccgcccca
     3241 ggcgctcccg caccgccacg aggtccgcct ccgccgacga ggcatcgaac gtgacctcga
     3301 acacgcgcgc gccggcatcc gcgagctcgt cgaccatctc gagcaacgcc gccaggggcg
     3361 tcacgcgccg gagcacgacg acgagccggt gctcccggat cgccgcggcg atcggccccg
     3421 cgcggaggtc gtcggccttc atccgaccga cgccccggtg gccacgggct cgagcagggc
     3481 cccatcgtcg aggagccgct cccggatcct cgaagcgtcg agcgcgcgcg gcgtcgtctc
     3541 cccggccgcc gccatcgccg ccgccgttcc tgccgcctgg cccatcgcca tgcaggtcgc
     3601 catcgaccgc gccgaggcat gcgcatcgtg ggtcgccgag aagcagcgcc ccgccatcag
     3661 caggccctcg atcgcggcgg gcagcaggca ccgataggga atgccgtaca cgcccggctc
     3721 gcccacgtag tgccacgccg tgtcgccgcc cgtgccgtgg tcctcgatcg gggcgccgca
     3781 cagggcgatc tcgtcgtcga accgccggcc gccgagcacg tcctcgcgcg tcaggcggta
     3841 gtcgccgtgg acccggcggc tctcgcgcac gccgatcgcg ggcgacgtgg cgacgaccac
     3901 ggagcgctcg aatcccggca cgcggtccct caggaagcgg tggtactcct gcacctggcg
     3961 ccggccctcg atctcggccc gggtcagctg ggccggatcg gtcgcgtcga cgttggggat
     4021 gcgggtcatg tggatgagga ccacgccggg gtacggcgtg cggtgccacg agccctcgac
     4081 ccgcggcagc cggtagccgc cgtgctcgac ggcgtcgcgc atcagcgccc acagctcggc
     4141 cttcgggacg gccgttgcgc gttcgatgtc cacgttggcg agcttgaaca gggtcgagag
     4201 cgactgcacg ccgccgggcg tggagctcgc gtcgtcgtag ccggcgccgg cctgcgcgca
     4261 cacgtcggcg tcgcccgacg cgtcgacgaa cgccgccgcc tcgacccagc gctcgccgcc
     4321 cttgttccac aggcgcaccg ccctcaggcg ccggtcgccg tcgatccgga cccccgtcgc
     4381 ccacgtatgc agcaggagct cgacgcccgc gtcctcggcc agccgctccc agaggacctt
     4441 gagcgcctcc gcgtcgtagg tgacgccggt gcccgcgccg tacgtgttcg gccgctcgaa
     4501 cgcgacgccg ttcgcggtca gcgcgctcgc gacctcccag ccgagcccgc cgaccacgcg
     4561 ccgcgggtgc gtgcccggcg tgtagaaggc atagaacgtg tcgagaaccg cggtcgaggt
     4621 gccgcccatg aaggcgagac ggtcgaggag cagcgtccgt gctccgtgcc gcgccgccgt
     4681 gatcgccgcg gatgaccccg cggatcccga cccgacaacg acgacgtcgt agcgatcggt
     4741 cacgacggcc cggctcccgc gatggcgtac aaccgcgccg ggttccgcac gaggagccga
     4801 tcggtcacgt cgtcgccgac ccggcgccgg agtcgcggga caaaggtccg catcaggtag
     4861 cgcatgcccg ggccgccgtc gtaggcgcgc aggtagtcgc gggcaccgag gtccaggccg
     4921 aggacgatgc gatcggtcat gttgttggtg acgagggcct cgatcaggtc gagcacgacg
     4981 ctgtcggggt ggtacttcgt gcggccgatg gtgtcgtact cgagcgtcac gccgcgcgcc
     5041 gcgatctcca cgtgcagctc ggcatcgggg ttgcgatcca tgtgggcgag cgcgatccgg
     5101 tcggtcgcca gccccgcgga ctcgagcagg tcgacgacgt cgtgcccgga cgtcccgatc
     5161 tcggtgtgga cgaggaccgc cgcgccggtc gccgacgcgg cgcccgcgat cgcctccagg
     5221 cgccgctgct cgctgcgggt gatccggtgg tacgaggcgc cgcccttgat cgcgccggcg
     5281 cgagcggggt cgagggggag ccccgggtcg tcccagtccg acgggtgcat ccccgtcgtg
     5341 aggtcggtca cgatccggtc cgccagcgtc tccacggtcg ccgcgtggac ccagtggccg
     5401 tcggggtagt gggtgtcgcg gtgatagccg gacgcggcga tgatcgtgag cccggttgcg
     5461 tccgcgacgg cgcgcatgcc cgccggtcgc cgcccgagcc cgatcggcgt catctccacg
     5521 atcgtgccga tcccggtcgc cttgccctcg agcgcctcgg cgatcgagcg gtccggatcg
     5581 gtgaacgcct ggccaggcat cgccggcgag tcgaggaaca ggtgatcgtg ggcgtcgacg
     5641 acgccgagca cgccgacggg tcgaggcccg gagacgctga cgacgtgggc ctccggcacg
     5701 cgaacctcct gcggcgacca tcgacgggcg accgccatgg tacccgccgg atgtggcggc
     5761 aacgggatcg cgcgggggtc gatcggcggt accgattgcc ccaacgttcc cattggttcc
     5821 cgcgtgtttg ctgcgtaagg tcgccaaaag gccgggtatg acgcccggcc gacgcgtggg
     5881 agggcaatct gcccgtcatt cgcgatcagc caccggtggc ccgacgcccg cgagggcgcc
     5941 tcggccggtt cacgctgcgc tgggcggccc cgttcatcgg cgtcctgctg gcctacgcgg
     6001 tggtggcccc gggtgccgcc acggccgcga ccacgtggtc caggaacctg tacgtcaacc
     6061 gggcgttcat gtaccaggac ccgtatccca cggcctgtac cgcggccgcc gcgatgatga
     6121 tgctcaacac gatcgcctac cgcgagaccg gcggtgacgg gttcacctgg accccgaccc
     6181 ggatcaaggc ggacgccggc aaccagcgcg acatgctctc gatcctggcc tacgcgcggt
     6241 cgcacgacac cctccgggcg aagggccgcg gctccgatcc ccacggctgg cggaacgtcc
     6301 tcaacgcctt cggctggggc gaggatgcga tgcgggaccc ggcgaagcgg gtctacgacg
     6361 accgcgccta ccggacattc gacaaggccg tgaagtccgc ggttcgggcg atcgcgcggc
     6421 gctcgatgcc cgtgggcgtc ctcggctggg ccggtggtca cgcccaggtg atgaccggct
     6481 acgtggtcac cggcgccgac ccgagagtgt ccaacgactt tgtcgtgcgc tacgtctacc
     6541 tgagcgatcc gctggccggg aactcgaccg tcaaccggag gctcagcctc gcgaagttcc
     6601 gctcgggccc gctgcggacg cggttccagg cctatcgcga ggccgacagc ccctacgacg
     6661 acccctggac gccggggaac atcaagagct ccgtcgtgcc cagccgcggg cagtccgagt
     6721 ggtaccaccg ctgggtcatc ctgcgaccga ccacgcccgg tgggcagacc cccgacccga
     6781 cgccggatcc ggatccaacg gcgtcgccgg accccacacc gacgcccgac cagggcgggg
     6841 aacccgatcc caccgcgacg ccggactcga ccgaggcgcc tgaaccgacg ccggacgagg
     6901 ccgccccagc cgaggagccg gccgactcct gatcgcgccg cgtgcgaacc ggccttgaac
     6961 cgcgggcgta ccgtgggtac aggtcgatga acgtcgcgcg aggaggcatc cggtgcgctc
     7021 gtatctgatc gtggccaacc gtacgctggc cagtcccacc ctcgccgccg ccgtcgccca
     7081 gcgcgtcgcc gaaggcgagg caaccttcca tgtcgtcgtg ccgcggacgc cggtccagca
     7141 cggcctgacg tgggacgagg acgaagccga ggccgccgcg cagcagcgcc tcgacgacgt
     7201 gctcgcccgc ctgaagtcca tgggcgtggc ggcgaccggc gaggtcggca gcaaggaccc
     7261 cgtcgacgcg gtccatgacg cgctgcgcgg gcgcgaggca gatgagatca tcctgtccac
     7321 gttgcccgtg cgcgcctcgc gctggctcgg tcaggacgtc ccgaccaagc tccggcggag
     7381 cgtgaacgta ccggtggagg ttgtcatcgc tcccgacgaa ggcgcgccgg ctgcggccgg
     7441 ctccggcgac caggcagccg acgatcaagc gcaccgcgtc gccgaccgct gaatcctgcg
     7501 tccacgacag cgatgaccga ggggccgatc tatcccgttg atccgtggcg gatccgcgag
     7561 ctgaccttcg accccgcgct ggcgcctcgg aacgagacga tcttcgcgct cggcaacggg
     7621 cacctgggca tgcgtgggaa cctcgacgag gatgccggca acgccgatca cgggacgtac
     7681 gtcaacggct tctacgagga agcgccgatc gtctaccccg agggcgcgta cgggtacgcc
     7741 cgcaaccacc aggtcctgct caacgtcgcc gacggcaagc gcatcgcgct gttcgtgggc
     7801 gacgacgcgt tcgatctcac caccggcacg atcgaggcgt atgagcgatc gttggacctc
     7861 cgcgacggca tcctgacgcg gaccgtccgc tggcgatccc cgggcggcgc gacggtcgac
     7921 gtcgtcgccc ggcggctggt gtcgctcgcc cggccggccg tcggtgcgat cgacttcgcg
     7981 gtgacgctgg tcgagggcga tgcccggatc cgcatcgtgt ccgcgatcaa cgcgcgcgtc
     8041 aggaaccagg aggcctccga cgatccgcgt gtcggcgcac acctcccggc gaacgtcctc
     8101 gagacgatcc accacgaggc atcggggacg ttcggcgccg tcgtccagcg gacccaggca
     8161 acgcggctcg cgatcgtggc cgcggccgat catgtcgtcc gcagcgacgg agcgtccctt
     8221 gacggccagt cgtcgacggc ggacgagggc gggttcgcgc tgaccctcga cgcgtcgctg
     8281 gcgccgggcg ggtcgctcgg ggtgaccaag ctgctcgcct actgcacctc gcgcgaccac
     8341 cccgaggaag cgctcctcga tcgcgcgcgc gacgcgatcg gcctggcgcg cgagaccggg
     8401 ttcgacgggc tcgccgccga gcagcgggcg gtcctcgacg ggttctggag cgcctcggac
     8461 gtcgagatcg acggggacga gatgctgcag cagggcgtcc gattcaattt gttcagcctg
     8521 ttccagtcgg ccggccggga tggccggacg agcctggccg cgaagggcct gtccggcgag
     8581 ggctacgagg gccactactt ctgggacacc gagatcttcg cgctgccgtt cttcggctat
     8641 acccagccgg ggatcgcgcg ggccctgctg cgctatcgct gcgggatcct ggacaaggcc
     8701 cgagcgcggg ctgcggagat gagccagcgc ggcgcgctct acccgtggcg gaccatcggc
     8761 ggcgaggagg cgtcggccta cttcccggcc ggcaccgcgc agtaccacat caacgccgac
     8821 atcgcgtacg ccatcgagaa gtacgtcgcc gccagcggcg accgctcgct gctgctcgag
     8881 ggcggagccg aggtcgtgtt cgagaccgcg cggctgtggg ccgacctcgg cgactacatc
     8941 ccggcgcgcg acggtgcctt ctgcatcaac gaggtcaccg gacccgacga gtacacggcc
     9001 ctcgtcaaca acaacggcta tacgaacctg atggcgcgcg cgcaccttcg cttcgccgcg
     9061 cggctcgtgg acgagctggc ggccgacgag cccgccgcct acgacctgct cgccgcgcgg
     9121 atcgggctgg agcctgccga ggtggccgag tggcgccgcg ccgcggacct catgcgcatc
     9181 ccgcacgacg ctgccctcgg cgtccatgcg caggacgact cgttcctcga ccgcgccccg
     9241 tggccgttcg cgacgacgcc ggcctcggag taccccctcc ttctccacta ccacccgctg
     9301 gtgatctacc gccaccaggt gctcaagcag gccgacgtcg tgctcgcgca ggtcctgctc
     9361 ggcagccagt tcacgaccgc ggagaagaag cgcaacttcg actactacga cccgctgacg
     9421 acgggcgact cctcgctgtc gccgtgcgtc cagagcatcg ccgccgccga gctcgggtac
     9481 gcggacgcgg cctaccgcta cttcatgcgc accgcgcgga tggacctcga cgacgtcaac
     9541 ggcaacgtcg cccacggcgt gcacgtcgcc gccatggccg ggtcgtggat gtcgctcatc
     9601 tacgggttcg ccggactgcg cgatgacgac ggctcgatcg ccttctcgcc ccggctgccg
     9661 gacgcctggg accggcttgc gttccggctc gccgtcggcg gcgcgaccct cgcggtcacc
     9721 atcacccgcc acgagatcag ctacgcgctg gtcgggggcg acgcgctcgc catccgccac
     9781 ttcggccgac cggtcgagct gacggccgcg gccccggtgg tgatcggcct cgatcccaac
     9841 ctgcgggccg tgatcttcga cctcgacggc gtcatcacgg acaccgccga gcaccactac
     9901 cgggcctgga agcggctcgc cgccgaggag tcgatcccgt tcgaccgcga gctcaacgaa
     9961 cggctcaagg gcgtgagccg gatggagagc ctggacatca tcctcgaagg cgcgggccag
    10021 acggccagcc tggccgacag ggtccggctc gccgaccgca agaacgccta cttccgggaa
    10081 ttgatcgacg cgatcacccc ggccgacctg ctgccgggca tcggcgacct gctggctgac
    10141 ctgcgcgcac gcggcgtccg gagcgcgatc gcgtcgatga gccacaacgt gtacgacgtc
    10201 gtccgccacc tgggcatcga ggcgatggtg gacgtcatcg tcgacccggc gacgctcgtc
    10261 aagggcaagc ccgatccgga gatcttcctg gccgcggccg acgtgctcgg catgcgctac
    10321 gaggactgcg tggggatcga ggacgccagg gccgggatcg ccgcgatcaa ggccgcgcgc
    10381 atggtcgcgg tcggggtggg ggacgacctg ccgggctgcg actggctcat ctcggacacc
    10441 cgcccgctga cggtcgacgc cctcgccggg ctgttcaggc gtgcggaggc cgctggccgg
    10501 gccacggatc cggcggagcg aatcgtctcg gcggatcccg tcgcctagcg ggagatcccc
    10561 tctggtcaac tgcgatttga cggcgtagga tgcgccgaaa ccggcgcacg actcgcgccg
    10621 gcgcgatcgc caggacacga cgatcgctag cgaggcggcg cagtcgcctg cctcatggag
    10681 tgtgccgggg tggggagcct ccacgtcgcc ctgctggacg gattcgagga cgacaccgtc
    10741 gaggtgctgg tcgcggacca ggtcgtcgcc cgacggtccg gcgtgacgac gatgacccag
    10801 atcagccgcg ccgacgagtt cgacgtccag ctcgggggag cgacgacgct ggtcgtccgt
    10861 ctcccggagc gcggcctgca ggcgaccgtg gacctcggtg gtgttgggtc ggcgaccaac
    10921 cttggcgtcg ccgtcaccgg ggacgcgatt cagacccgga tctcggcgga gccattccgc
    10981 ttcgcctgaa aacaccacgg aaggggggtg gtgccagcag cgagtcgcac cgtcagtgca
    11041 cgtagctgag gagatcctcc atggccatca aggtcgagcc gcagccggag actgcggcgc
    11101 tcacgggctc cgaagaggag gccttcggca tccccttctg gccgcccttc cggctcgccg
    11161 cgagcgggct ctacacgtgg cggttcccca tctcgatcat ccccaggcag ccgatccctc
    11221 tgcccaagct gccgcccatc ccggatcccg gaccctggcg cggcggcgac gtcaccgagg
    11281 ccggcgacgg ccaggcggtc gacaccgcaa cctctgccct gttcccgatc ctctccgagc
    11341 ggatccgcct cgacgtcgat ggccgctatc cgcagatggt cgtcagcggg gacatgtcga
    11401 cgggcctgtt ctcgacggtg cactggatcg cgaacctgac gaagaccggg tcgaacacct
    11461 acgtcggcgc gatctggtat cgggacccgc ccgtggcgcc gttcccgtac gacagcgtca
    11521 aggtcacgac gggcggcggc tggctgttcg atccgcgaac agcgaccgtc acgttcagcg
    11581 gacctggcgg catccagcgc acgcggactt acacctacac gtcaccctac ttccacgacg
    11641 tcgacctcga ggtcgacttc cagaccggcg tgacgccggc cacgagcttc gccacgtgct
    11701 cgcaccccaa ccgaccggcg acgctgccgt gcgagacgct cacgatcggg aaggtctttc
    11761 agcgcgcggg cttcaacgtc acgtcctcgc cgagcggaac ggtgcccgtc gccggcgccg
    11821 gggcgaacgc gacctggagc aaccaggaga tgcacgacgc gatgcaggtc tactggtcgc
    11881 attacgcccc caaggcgcag tgggcgacct ggatcttctt cgcggcaacg cacgacatgg
    11941 gccacggcct cggcgggatc atgttcgacg acatcggccc gcagcagcgc cagggaacgt
    12001 cgatcttcat cgactcgttc atctcggatc cggcgccggc gggcgatccc gcccccgcgg
    12061 ccttcatcga ccgcatgcgg ttctggaccg gcgttcacga gacgggccac gcattcaacc
    12121 tcgcgcacgc gtggcagaag tcgctcacgt cgggtggcaa gggcccctgg ctgccgctcg
    12181 tcgacgagcc cgagtcgcgc tcgttcatga actacccgta cttcgtggcc ggcggcacga
    12241 ccgcgttctt cgccgacttc gagtaccggt tcagcgacca ggagctgctg ttcatgcggc
    12301 acgcgccgtc cagcttcgtc cagcagggca acgccctgtg gttcgaccat cacggcttcc
    12361 tcggcgccaa cgccctgccg gagccgacct accagctcgt cgtccgcgcg aatcgtgacc
    12421 gaccttcgtt cgagttcatg gagccggtca cgctcgagct caagctcacc aacgccacgg
    12481 ctcagccgca gatcgtggac gagcacatcc tggacgtcga tcaggggctc gtggtgatca
    12541 tcaccaagaa gggggagccg acccgtcagc tcctgccgat cgctcgcctc tgccgtcagg
    12601 ccagtcagaa ggtgctccag cccgcggagt cggtgtacgc gccgatgctc gcgtcggtgg
    12661 ggccgaacgg ctggaacatc tcggaccccg gcacgtacgg gatccaggtg tgcctgcatc
    12721 tgccgacggg cgaggacatc ctgtcgaacg agctccgcgt catcgtcgag ccggcccgca
    12781 cgacggagga cgagaccctc gcgcaggact acttcaccga ggacgtgggg agggtgatcg
    12841 ccctcgaggg cagccgcgtg ctggacaccg cgacggacac cctgacggag atcgccgaca
    12901 agctccgcca tcggcgggtc gccgtccacg cgaacctggt cctcggcgtg ccgctggcca
    12961 ccgagtacaa ggtcctgtcg cgcaaccgca agcacgacgg gcagcagtac cggatcgagg
    13021 cgctgcccgc ccggccggaa gaggccaagg accggctcgt ttcggcgctc agcggggacc
    13081 cgacgcgctc cgcggagtcg ttcggtcacg tccgctacaa ggagtccgtg gaccagctca
    13141 ccgggttcct cgagtccgcc ggcgacgccg cggacgccta caagcagcag gacatcatgc
    13201 tcgagaccct gtccgggcgc gaagtgcgcg gccgcaaggt gctcgactcg gtcctcaagg
    13261 agatcaagac gaagcgcgac agcctcgcct cggcggcagg cgtcaagtcg cgctagccac
    13321 gagcagcggc cggcccgtcc gccgtcccgg cggcgagccg gccatccccg ccccttcgcg
    13381 cctgagcgcc gcggccgggc gggccgcgtc agccgccggc catcgccggc aggagcagca
    13441 ccgtgtcgcc ggggccaagg ggcgtcgcca ggtcctggag gtggcggacg tcggtctcgt
    13501 tcacgaacac gttcacgaac cggttgatcc cgccggacgc gtcgagcagt cgtggcccga
    13561 ggtccgggta ggcggcgacg agcgcgccca gcgcttcgcc gacggtcgcg ccctcgacct
    13621 cgatcagctt ctggccgccg gccgacgtcc ggagcaccgg cgggatgcgg acctggctca
    13681 tgccgacagc cagtcttcga acgccgcgaa ccgggccggg atgacgggtg tgagcccggg
    13741 ccgcccgggt tcggcaggcc gatcctcgat gccgaaccgg accgcgtccg gcgtcttgag
    13801 gccgttgccc gtgagcaggg cgaccacctc gtcgccgtcg cggatcgtcc cgttgcggcg
    13861 cgcggcctcg gccgcggcga tcgtcacgcc gccggcggtc tcggtgtaga cgccctcgag
    13921 cgtcgccgtc cggcggatcg cgtcggcggt cgcctcgtcg gtgatggcct cgatcgaccc
    13981 ctccgatgca cgcgcgagct ccaccgcgta gcggccgtcg gccgggttgc cgatggccag
    14041 cgatcggacg atggtgtcgg gcgtcctgac gggctcgatg tccgagcgac cggccgcaaa
    14101 ggccgtcgcg accggggcgc aaccggcggc ctgcccgccc acgaaccgga ccggcttcgc
    14161 ggccacccag ccgagctcga ccagctcctc gaacgaccgg gccagccggg tgaccatcgc
    14221 gcccgaggcg atcggggcga cgatgacgtc gggcaggcgc cagccgagct cctcggcgat
    14281 ctcgtaaccg agggtcttgc tgccctcggc gtagaacggg cggaggttga cgttgacgag
    14341 gccccagggc agctcgtcgg cgacctcgag gcacaggcga ttgacgtcgt cgtaggtgcc
    14401 gtcgatccgg acga
//
LOCUS       DBNHXO010000022         4324 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4965760, whole genome shotgun sequence.
ACCESSION   DBNHXO010000022 DBNHXO010000000
VERSION     DBNHXO010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4965760"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1980
                     /locus_tag="AC162A_00485"
     CDS             <1..1980
                     /locus_tag="AC162A_00485"
                     /inference="COORDINATES: protein motif:HMM:NF016563.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-lysosomal glucosylceramidase"
                     /protein_id="HIR5009343.1"
                     /translation="GGGTYHGLFPRAWQAFEPETLGVRLVGEQLSPVIGGDLERSALP
                     VGVFEWWIENPGPDPLTVGLLFTWADPFAASGPAAPRPHRVVAGDDGSLAVEFGDAAD
                     DAPTALRGTLAIAALGGDGMQLSARSTFDPVADRELWSDFAADGRLEPGGGPSEGGRQ
                     ADRASGAALAATVELRPGERRSVRFAIAWDLPVVEFGAGRKWWKRYTKAWGRTGDRAV
                     DLARHALAEAPAWRAAIEAWQAPYLEDPGRPDWYAMALFNELYFLVDGGSFWEHGEVG
                     GPVPGPGDVGRFALLECVDYPFYDTVDVDFYASFALLEVFPELELRGIRDLLEAIPVD
                     LPEIVTIEASGATAPRKVGGTVPHDVGGPTDDPFIRPNWYTFQDVNDWKDLGPKFVLQ
                     AWRDALAAGPDGDALIEAVWPTVDAVLTRLSRFDRDGDGLLEHDARPDQTYDTWPMRG
                     PSAYAGSLWLAAVLAAEQMARRLGHGEAAARWEGWFERGQVAFDRRLWRGDHYAYDDG
                     AGPSSDSIMADQLAGQWYADVTGLGDIAPPDQVEAALRTIHRMNVHTFADGRMGAVNG
                     MRPDGTVDHSSEQSAEVWVGTAYALAAFMIGRGLDAEGWETARGVVATTYERGLWFRT
                     PEAYDRDGNFRASIYLRPLAIWAIEEALRRRAARP"
     gene            complement(1993..2394)
                     /locus_tag="AC162A_00490"
     CDS             complement(1993..2394)
                     /locus_tag="AC162A_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009344.1"
                     /translation="MTRALVVHHDIDLADQEVESLRRFGYQVEQCSGPTANTCPILNG
                     GSCDLAARCDVLVYDAWASGDTDGSGALIDGLRRVHPTVPIVLTVPGVGMRWEPTSDP
                     GVVELQGQPTGARLHEAIQRAIEGGREPVER"
     gene            complement(2602..3435)
                     /locus_tag="AC162A_00495"
     CDS             complement(2602..3435)
                     /locus_tag="AC162A_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009345.1"
                     /translation="MIISDEHGYVFVEQPHTACTAIRDELVAHYGGRSILDRHATYAA
                     FRRVATPAQKRYFVFSGMRNPLDERVSLYCKFRNDHHHKYSRQFRRMAERQQHAFVEV
                     ADEHADFGTFLRHYNRRPYDNDMLIFHARMDDLIRFEHLQEDFSRVLRRLGLRQVRPV
                     PVVNATAERGSFLDYYPPELRGHAVRVFGPFMRRWGYELPPEWGDVRVPIAWTVGYRV
                     LGAVRYVYRRYLNVGRLNVRRRATNPVLRRVDSIVGPLKRTAYQLFGPLLASVGLIAL
                     S"
     gene            complement(3494..>4324)
                     /locus_tag="AC162A_00500"
     CDS             complement(3494..>4324)
                     /locus_tag="AC162A_00500"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR5009346.1"
                     /translation="DEVILHEPSYVAYVPAIVFAGGVVRHVATSAANDFALDPAGVGA
                     AITSRTKALFLGYPANPTGAVLDDDVQDELARIAERHDLLVYSDEIYDRLAYGTYRHR
                     AFSSLPGMRERTILMGGFSKAYAMTGWRVGWLCAPAGILEGIVKVHQYGIMSASTVAQ
                     DAALAALQAGEPAVVSMVAEYDRRRRLLVDALNRFGLPTFEPRGAFYAFPDISSTGLT
                     SDEFTERLLREEKVAVVPGDAFGPSGAGHVRMCYATAYEQLEEAIARIGRFVERVRPA
                     "
BASE COUNT          616 a         1512 c         1573 g          623 t
ORIGIN      
        1 ggcggcggga cgtatcacgg cctgttcccg cgcgcgtggc aagcgttcga gcccgagacg
       61 ctcggcgttc ggctcgtcgg cgagcagctg tcgccggtca tcggcggcga cctcgagcgg
      121 agcgcactgc ccgtcggggt gttcgagtgg tggatcgaga accccgggcc cgacccgctg
      181 acggtcgggc tcctgttcac ctgggccgat ccgttcgcgg cctccggccc cgcggcgccg
      241 cgcccgcacc gcgtggtggc cggtgacgac gggagcctcg cggtcgagtt cggtgatgcc
      301 gccgacgacg cgcccacggc tcttcgcggg accctcgcca tcgccgcgct cggtggggac
      361 ggcatgcaac tcagcgcgcg ctcgacgttc gatccggttg ccgaccggga actttggtcg
      421 gacttcgccg ccgacgggcg gctggagccc gggggtgggc catcggaggg cggtcggcaa
      481 gcggatcggg cgtcgggcgc cgcgctggcg gcgacggtcg agcttcggcc cggcgagcgg
      541 cgctcggtgc ggttcgcgat cgcctgggac ctgccggtcg tcgagttcgg cgccgggcgg
      601 aagtggtgga agcgctacac gaaggcctgg ggccggacgg gcgatcgggc ggtcgacctc
      661 gcgcggcacg ccctggcgga ggcacccgcg tggcgcgccg cgatcgaggc ctggcaggca
      721 ccgtacctcg aggatccggg acgcccggac tggtacgcga tggcgctgtt caacgagctc
      781 tacttcctgg tcgacggcgg ctcgttctgg gagcacggcg aagtcggcgg tcccgtgccc
      841 ggtccgggcg atgtcggccg gttcgcgctg ctcgaatgcg tcgactaccc cttctacgac
      901 accgtcgacg tcgacttcta cgcgtcgttc gccctgctcg aggtgttccc cgagctggag
      961 ctgcgcggga tccgggacct gctggaagcg atccccgtcg acctgcccga gatcgtgacc
     1021 atcgaggcgt cgggcgcgac ggcaccgcgc aaggtcggcg gcacggtccc ccacgacgtc
     1081 ggcggcccga ccgacgaccc gttcatccgc cccaactggt acacgttcca ggacgtcaac
     1141 gactggaagg acctggggcc caagttcgtg ctccaggcgt ggcgggacgc cctcgccgcg
     1201 gggcctgacg gcgacgccct gatcgaggcc gtgtggccca cggtcgacgc cgtcctcacc
     1261 cggctgtcgc gcttcgaccg cgatggcgac ggcctgctcg agcacgatgc tcgccccgac
     1321 cagacctacg acacctggcc gatgcgcggc ccgtccgcct acgcgggctc actgtggctg
     1381 gccgcggtgc tggcggccga gcagatggca cgccgcctgg gccatggcga ggcggcggcc
     1441 cgctgggagg gctggttcga acgcggccag gtcgccttcg atcgccggct gtggcgcggc
     1501 gaccactacg cgtacgacga tggcgccggc ccgagctcgg acagcatcat ggccgaccag
     1561 ctggcgggcc agtggtacgc cgacgtcacc ggcctcggcg acatcgcccc gcccgaccag
     1621 gtcgaggccg ccctgcggac gatccaccgg atgaacgtgc acacgttcgc ggacgggcgg
     1681 atgggcgcgg tcaacgggat gcgccccgac ggtaccgtcg accactcaag cgagcagtcg
     1741 gccgaggtct gggtggggac cgcgtatgca cttgcggcct tcatgatcgg gcgcggactc
     1801 gacgcggagg gctgggaaac ggcgcggggc gtcgtcgcga ccacctacga gcgcggactg
     1861 tggttccgga cgcccgaggc atacgaccga gacggcaact tccgcgcctc gatctacctg
     1921 cggccgctcg cgatctgggc gatcgaggaa gcgctgcggc ggcgcgcggc tcgtccctag
     1981 ggcgaggcgg acttagcgct ccaccggctc ccgtccgccc tcgatcgccc gctggatcgc
     2041 ctcgtggagg cgggctccgg tcggctggcc ctggagctcg acgacgccgg gatccgatgt
     2101 cggctcccag cgcatgccga cgcccggcac cgtcaggacg atcggcaccg tcggatggac
     2161 acgccgcagt ccgtcgatca gcgcgccgga gccgtccgtg tcgccgctcg cccaggcgtc
     2221 gtagacgagg acgtcgcacc gcgccgcgag gtcgcagctg ccgccgttga ggatcggaca
     2281 cgtgttcgcg gtcggcccgc tgcactgctc cacctggtag ccgaatcgcc gcagcgactc
     2341 aacctcctgg tccgccaggt cgatgtcgtg gtggacgacg agcgctcgag tcatggcgca
     2401 cctcgatcag tccaccggga gagcggccgc cgcatggcgg tcacgccgga tccggcaacg
     2461 gtgctcgggg ggcgccattc gcgatagtga ccaaggtcat ccgtgggagg ggcattccgc
     2521 cccccggtcg acctgcccga atcgtcgggc accgggggtc tgccgattgc gttcccgatg
     2581 cagccgcgcg ggtacctggc ctcagctgag cgcgatcaga ccgacggaag ccagcagcgg
     2641 cccgaacagc tggtaggcgg tccgcttgag cggtccaacg atcgagtcga cgcggcgcag
     2701 cacggggttc gtcgcccgcc gccgcacatt gaggcggccc acgttgaggt agcggcggta
     2761 gacatatcgg accgcgccca ggacgcggta gcccacggtc cacgcgatcg gcaccctgac
     2821 gtcgccccac tccggcggca gctcgtagcc ccagcggcgc atgaacggcc cgaacacgcg
     2881 gaccgcgtgg ccgcgcagct cgggcgggta gtaatcgaga aaactgccgc gctcggccgt
     2941 ggcattgacc acgggcaccg gccgcacctg ccgcaggccc aaccgccgca ggacacggct
     3001 gaagtcctcc tggaggtgct cgaaccggat caggtcgtcc atgcgcgcgt ggaagatgag
     3061 catgtcgttg tcgtacgggc gtcggttgta gtggcgcagg aacgtcccga agtccgcgtg
     3121 ctcgtccgcg acctcgacaa aagcgtgctg ctgacgctcc gccatgcgcc tgaactggcg
     3181 gctgtacttg tgatggtggt cgttccggaa cttgcagtac agactcaccc gctcgtcgag
     3241 cggattccgc atgccggaga agacgaagta ccgcttctgc gccggcgtcg ccaccctgcg
     3301 gaacgccgcg tacgtcgcat gccggtcgag gatcgaccgg ccgccgtagt gcgcaaccag
     3361 ctcgtcccgg atcgccgtgc acgccgtgtg cggctgctcg acgaatacgt agccgtgctc
     3421 atcactgatg atcacggata tcgattgtag ggcttcgatc aaccccctgt cgggtctccg
     3481 ggcacggcgt gcctcaggcg gggcgcacgc gctccacgaa ccggccgatc cgggcgatcg
     3541 cctcctcgag ctgctcgtag gccgtggcgt agcacatgcg cacgtgcccg gcgccggacg
     3601 gcccgaacgc gtcgcccggc acgaccgcga ccttttcctc gcgcagcagg cgctccgtga
     3661 actcgtccga ggtcaggccg gtggagctga tatccgggaa ggcgtagaag gcgccgcggg
     3721 gctcgaacgt cggcaggccg aaccggttga gggcgtcgac caggaggcgc cgccgccggt
     3781 cgtactcggc gaccatggac acgacggccg gttcgcccgc ctgaagcgcg gccagggcgg
     3841 cgtcctgggc aaccgtggac gccgacatga tcccgtactg gtggaccttg acgattccct
     3901 cgaggatccc ggcgggcgca cacagccagc cgacccgcca gccggtcatc gcgtaggcct
     3961 tggagaagcc gcccatcagg atcgtccgct cgcgcatccc cggcagcgag ctgaacgcgc
     4021 ggtggcgata cgtgccgtac gcgaggcggt cgtagatctc gtcgctgtac acgagcaggt
     4081 cgtggcgctc ggcgatccgg gcgagctcgt cctgcacatc gtcgtcgagc acggcgccgg
     4141 tcgggttggc ggggtagccg aggaacaggg ccttcgtgcg cgatgtgatc gcggccccga
     4201 cgccggccgg gtccagtgcg aagtcgttcg ccgcggacgt ggcgacgtgg cggacgacgc
     4261 cgccggcgaa cacgatcgcg gggacgtacg cgacgtacga gggctcgtgg aggatgacct
     4321 cgtc
//
LOCUS       DBNHXO010000023         3506 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2584292, whole genome shotgun sequence.
ACCESSION   DBNHXO010000023 DBNHXO010000000
VERSION     DBNHXO010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2584292"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="AC162A_00505"
     CDS             <1..844
                     /locus_tag="AC162A_00505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5009347.1"
                     /translation="GKNVKDVAGYGLMPLLVGSQGTLGLITEATLRLRPTPPPRATML
                     AFFSSLDAAGQAVAGMTAAGLGPVTLELMDGFTIRAVDDVHKLGLDRDAAAMLLVESD
                     LPGGAATSEMDAAEPICEAAGATLVVRAADPQEADWLRQGRRLAFRALEHLGVARMED
                     VGVPRSRVPEMLREIETIGERHRLTIGTFGHAGDGNLHPTFVLDRNEDEAASVPRLEA
                     ARNDLYEAALALGGTVTGEHGIGVARRDWLVRQRGEDAVAVMRAIKHALDPQDLLNPG
                     RVLV"
     gene            complement(873..1886)
                     /locus_tag="AC162A_00510"
     CDS             complement(873..1886)
                     /locus_tag="AC162A_00510"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR5009348.1"
                     /translation="MVTNPDWPTALDTVAAESSFSGLIGIDRPGRTPVDRAIGLADRR
                     WEVPVKPDSILAIASGTKGFTALTVMSLVTNGTLSLDSTARSILGADLPDVAGDVTVR
                     HLLGHRSGIGDYLDESEVDVTDHVLPEPVHRYLDAEDYLPSLDGHPTVFPADERFAYN
                     NGGFVLLAILAARAAGVPYHQLVHDRVIAPAGMVDTAFQRSDAPEPRTATNYLAADGL
                     RTNQLHMPLRGVGDGGLFSTLADMRSFWAALLAGRIVPPDVLAEMVEPRSESPDDDRR
                     YGLGFWLPAGGDAIQLEGYDAGISFLSTCRPSTGAVVTVISNWTDGIWPIVSFLREVA
                     TDG"
     gene            1950..3251
                     /locus_tag="AC162A_00515"
     CDS             1950..3251
                     /locus_tag="AC162A_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009349.1"
                     /translation="MEARILPLPTPALRSAGPDGAAPKVRVEGDRIVIDRLVLTDRVL
                     ASSLAEREPAERPDVIERALRIGLLAIQDASTTMDVDVVRREFEKLVAQTSAVNEHAA
                     RAVEDVLRSNFADGDGRLPRTLEKFLGDKGALQQFVNELFDETRRDSAIGRIGGLLGQ
                     YFDGDRSKVAQLLDPTRMGSPLHQFRQEIADGFKGVHERLSSLEAASKARADERSRSA
                     AKGGDFEDLLEDLLGSLARGAGDLIDRTATDTGALLGSKKGDFVLTVDPRIARGADLR
                     IVVEAKDRAMSQRAMREELREARENRGAAVAVAIWTPAHAPTGIAPFSMVGDDVHVVV
                     DPEAPDTAYLEAAVRLARLLALASLQEHEVDVDAAAIGRALAGVREQLDAIRALKTQL
                     TSVSNATKAVWTGLDTLRNGILARVTEAEAELRVTGSTTAA"
BASE COUNT          507 a         1241 c         1293 g          465 t
ORIGIN      
        1 cggcaagaac gtgaaggacg tggccggcta cgggctcatg ccgctgctcg tcggcagcca
       61 gggcacgctc gggctgatca cggaggcgac cctccggctc cggccgacac ccccgcccag
      121 ggcgacgatg ctggcgttct tctcgtcgct cgacgcggcg ggccaggccg tggccggcat
      181 gaccgccgcg ggcctgggcc ccgtgaccct ggagctcatg gacgggttca cgatccgggc
      241 ggtcgacgac gtgcacaagc tcggtctcga ccgggacgcc gccgcgatgc tgctcgtgga
      301 gtcggatctc cccggcgggg cggcgacgag cgagatggac gccgccgagc cgatctgcga
      361 ggccgccggc gcgacgcttg tcgtgcgggc ggcggatccc caggaggccg actggctgcg
      421 ccagggacga cgcctggcct tccgggcgct ggaacacctg ggcgtcgcac gcatggagga
      481 cgtcggcgtc ccgcgcagcc gggtcccgga gatgctgcgc gagatcgaga cgatcgggga
      541 gcggcaccgc ctcacgatcg ggaccttcgg ccacgcggga gacggcaacc tccacccgac
      601 gttcgtgcta gatcggaacg aggacgaggc ggcgtccgtg ccgcgcctcg aggcggcgcg
      661 caacgacctg tacgaggcgg cgctcgcgct cggcgggacc gtgaccggcg agcacggcat
      721 cggcgtggcg cgacgggact ggctcgtgcg gcagcgtggc gaggacgcgg tcgccgtcat
      781 gcgcgcgatc aagcacgcgc tcgaccctca ggacctgctc aacccgggcc gcgtcctcgt
      841 ctgaatcgtg gcgccgtggc cctggtcgcg cgtcagccgt cggtggcgac ctcgcggagg
      901 aagctgacga tcggccagat gccgtcggtc cagttggaga tcaccgtcac gaccgcgccg
      961 gtcgacggcc ggcacgtcga gaggaacgag atgcccgcgt cgtagccctc gagctggatg
     1021 gcgtcgccac cggccgggag ccagaacccg aggccgtatc gccggtcgtc gtccggtgac
     1081 tcgctccggg gctcgaccat ctcggcgagg acgtcgggcg gcacgattcg tcctgcgagc
     1141 agggcggccc agaatgagcg catgtccgcc agggtcgaga acagcccgcc gtcgccgacg
     1201 ccgcgcagcg gcatgtggag ctggttggtc cggagcccat cggccgccag gtagttggtc
     1261 gccgtcctcg gctcgggtgc gtccgaccgc tggaacgccg tgtccaccat gccggccggt
     1321 gcgatcaccc ggtcgtggac gagctggtgg tacggcacgc cggccgcgcg cgccgcgagg
     1381 atcgcgagca ggacgaagcc gccgttgttg tacgcgaacc gctcgtcggc ggggaacacc
     1441 gtgggatggc cgtcgaggga ggggaggtag tcctcggcgt cgaggtagcg atggacgggc
     1501 tcgggcagga cgtggtcggt cacgtcaacc tcggattcgt caaggtagtc gccgatgcct
     1561 gaccggtggc cgagcaggtg ccggaccgtg acgtcgccgg cgacgtccgg caggtcggcg
     1621 ccgaggatcg agcgcgcggt tgagtccagc gacaacgtcc cgttggtgac gaggctcatc
     1681 accgtgagcg ccgtgaagcc cttcgtgccg ctggcgatcg cgaggatcga gtccggcttg
     1741 accggcacct cccatcgccg gtccgcgagg ccgatcgccc ggtcgacggg cgtgcggccg
     1801 ggccggtcga tgccgatgag gccggagaac gaggactcgg cggcgacggt gtcgagggcg
     1861 gtgggccagt cgggatttgt gaccatggcg gaagcgtagg gcacggcgtg acacgctagg
     1921 tgtcacagcg gggtccgagg atgcgcggca tggaagcccg aatcctcccc ctgccgacac
     1981 cggccctccg ctccgctggc cccgacggtg ccgcgcccaa ggtccgcgtc gagggcgatc
     2041 gcatcgtgat cgatcggctc gtcctcacgg accgggtgct ggcctcgtcg ctcgccgaac
     2101 gcgagcccgc cgaacgcccc gacgtgatcg agcgcgccct ccggatcggc ctcctcgcca
     2161 tccaggacgc ctcgaccacg atggacgtcg acgtcgtccg ccgcgagttc gagaagctcg
     2221 tcgcgcagac gagcgccgtc aacgagcacg ccgcgcgcgc cgtcgaggac gtgctgcgca
     2281 gcaacttcgc ggacggcgac gggcgcctcc cgcgtacgct cgagaagttc ctcggcgaca
     2341 agggcgctct gcagcaattc gtcaacgagc tgttcgacga gacgcggcgg gactccgcga
     2401 tcggccgcat cggtggtctc ctgggccagt acttcgacgg cgaccgctcg aaggtcgccc
     2461 agctcctgga ccccacgcgc atgggatccc cgctccacca gttccgccag gagatcgcgg
     2521 acggtttcaa gggcgtgcac gagcgtctga gctcgctcga ggccgcatcc aaggctcgcg
     2581 ctgacgagcg ctcacggtcc gcggccaagg gcggcgactt cgaggacctg ctcgaggacc
     2641 tgctcggttc gctggcccgg ggcgccggcg acctcatcga ccggacggcg accgacacgg
     2701 gagccctgct gggctcgaag aagggcgact tcgtgctgac ggtcgatccg cggatcgccc
     2761 gcggcgcgga cctgcggatc gtcgtcgagg ccaaggaccg ggcgatgtcc cagcgcgcga
     2821 tgcgcgagga actgcgcgag gcccgcgaga accgcggcgc cgccgtcgcg gtggcgatct
     2881 ggacgcctgc gcacgccccg accggcatcg cgccgttctc gatggtcggc gacgacgtcc
     2941 acgtggtcgt cgaccccgag gcgccggaca ccgcctacct cgaggccgcc gtccgcctgg
     3001 cgcggctgct cgccctggcg tcgctccagg agcacgaggt cgacgtcgac gccgccgcca
     3061 tcgggcgggc gctcgccggc gtgcgcgagc agctcgacgc gatccgggcg ctcaagaccc
     3121 agctgacctc ggtctccaac gccacgaaag ccgtctggac cggcctcgac acgctccgca
     3181 acggcatcct ggcgcgcgtc acggaggccg aggcggagct ccgcgtcacc ggttcgacga
     3241 ccgccgcctg atcgggcgcc ccggaccggg gagccatggg cctgcgtgac aagtaggcca
     3301 tacccatggg aggatggatg cgggaccatc gccccgagcc ggaagcaggc gttaccggcg
     3361 gtcgtgaccg ggagcccccc gatgcagcgc atcgcgcccc ttacccccct gttcctcatc
     3421 gccgcgctcg cagggtcatt catcgcgccc gtcgccgcga ccgccgatcc cggatcgacc
     3481 ggtggcgcgg tctcgaaggc agcgac
//
LOCUS       DBNHXO010000024         9828 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5234, whole genome shotgun sequence.
ACCESSION   DBNHXO010000024 DBNHXO010000000
VERSION     DBNHXO010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 9828)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9828)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9828
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5234"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1168)
                     /locus_tag="AC162A_00520"
     CDS             complement(161..1168)
                     /locus_tag="AC162A_00520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5009350.1"
                     /translation="MTTPIARLPEGPAGSDITYRRYRGVDDIDAMARANARLRALAGV
                     LEPIDLESMRHHYAHLVNSDPLVDCLIVERDGATVGYGRVEWHDLVDGDRSYDNVIVL
                     APDAWGAGIATDLVDWTERHSVEVARANATDRRAWFANYAFGRDDELNAALTERGYTA
                     VRWDAEMLRPDLENLPDVPAPDGYVLRSPTEAELPAVFAMSVEGFSEHWGQYEADEQN
                     YEEWVEDPRFRLDLVVVAWKGDEPAALVHNVLEDRPDGSVTGLLDGVTTHPRHRRLGL
                     ARACIAESLQRLRAAGASSAYLGVDTDNQNRAYALYESCGFRVATSGTSYRKPFTMET
                     P"
     gene            complement(1165..2280)
                     /gene="rsgA"
                     /locus_tag="AC162A_00525"
     CDS             complement(1165..2280)
                     /gene="rsgA"
                     /locus_tag="AC162A_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013921400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR5009351.1"
                     /translation="MAVSASPALQDLGWDPGWAAAFLPFDAAGWQPARVVAAHRNAWV
                     VATPSGDRDAVIAGRLRHEAVGAGDLPAVGDWVAVAGLGDGDTAVIQAVLPRRTAFGR
                     NTSADGYAGNLAGEQILAANVDVALVITSLDGDFNLRRLERYLAVAWTGGATPVILLN
                     KADVALDPDGLRVAAEAVAPGVEVRTISALTGDGVADLADHHLPPGRTAVVLGSSGVG
                     KSTLVNALLGHERQRTSAVRDDDARGRHTTTHRELIRLPAGALLIDTPGIRSLGVAGA
                     ADGLENAFADIGELAAGCRFRDCRHDGEPGCAVRAALDDGRLDAARFASHRKLEREAA
                     HVARANDPILRAAERRRWKAISASVAVHMKRKYGSEQ"
     gene            complement(2294..3097)
                     /locus_tag="AC162A_00530"
     CDS             complement(2294..3097)
                     /locus_tag="AC162A_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009352.1"
                     /translation="MTLLTHELKASYAFVERNFFLTRRYWGWEIAFLVYSVAGALSIT
                     LIGAAAGDTRLLLTLMIGAIFWNYLSVVFGWIAETIQVERWEGTLEYTFMAPIRRWTQ
                     LLGSCLYAMVFGLVHTGVILIVMMVFFRDLTLHVEDVPTALVFVLLGSFSIVGIGMMA
                     AILPLLYVERGAQMTFVLQSCLLLVSGVYYPISVLPEWMQVLSKFSPATYVLDGVRAG
                     LLDGVPVTELWYDVVPLIVMGIVLIPAGLWAFGRAERYAKRTGKLKRVG"
     gene            complement(3141..3401)
                     /locus_tag="AC162A_00535"
     CDS             complement(3141..3401)
                     /locus_tag="AC162A_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009353.1"
                     /translation="MTTLTAKHNGHTNRHHLTSRKDHRMFGIELRLAAINERQSLERS
                     FRDADRAAARQSRSIRSQVGESIIRLGRRVAGESLGSPALTG"
     gene            complement(3421..4374)
                     /locus_tag="AC162A_00540"
     CDS             complement(3421..4374)
                     /locus_tag="AC162A_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5009354.1"
                     /translation="MATTLVPEPKTTHPTSSPLPATETGLEPAARIPAGPDAGDANVL
                     PQPTPAGPPALLVEHVTKRFNVRRGKPPVLAIDDVSLRLERGTIQGVLGANGSGKSTL
                     IRLVSGLLTLDEGKVEVFGYDIERNELAVKRLINRVSVDAAFFKKLSPMENLLFAARL
                     YGLDGKQAKREATAILHRLGIAESRLGRPVEQMSRGMQQKVAIARALLTSPTLLLLDE
                     PTTGLDPRSKLDVQAYIEEVNAAHDATIVLTTHDLAEAERLCERITILDGGKTVAEDT
                     PEGLKRLVAEQHGQPPTLEAVFMTFTGRSLDEDVEEDDDND"
     gene            4955..5878
                     /locus_tag="AC162A_00545"
     CDS             4955..5878
                     /locus_tag="AC162A_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009355.1"
                     /translation="MVSNRQQSARIMAALATILLVAACAGASPGSGSTSASATLPAPT
                     PVGSGAAAEDYWLRLTTSQAIPPLDRFEVLPSLWITGDGHAVVPLVLPTPYPGPLVQG
                     LSGRTVSDAGRAAIVQEARDLGLLGGQTDFSSDAPLPGGVVGQIELVVDGTRVTLTGD
                     PSAHIECVTAPCDPPPGSAAAFGAFWNQLLDLPSWLGSDLGPPMPYDPPAYALVVGPP
                     PDPEPPLSPPPADWPLDQPLASFGARVANGTARCGTVSGADADALRPALDAANQLTPW
                     VQDPETSATFGLTVRPMVSGEDACKESFGVG"
     gene            complement(5886..6461)
                     /locus_tag="AC162A_00550"
     CDS             complement(5886..6461)
                     /locus_tag="AC162A_00550"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5009356.1"
                     /translation="MEIVPLSSGHWASLAELFSAGGDPKWCWCQFWRKPGSNWSNTTA
                     ADNRDDLWALMEGGGPSPGLVAIDDGKAVGWVGLGPREGFGRLARSRTLPQLPGEDVW
                     VINCFVVAKGSRRSGVARALLEAAVGYARDHGAGVLEGYPVATGGARISSASVYTGTA
                     AMFERAGFEVASPSTSKAASGTPRVVMRRAP"
     gene            6626..6823
                     /locus_tag="AC162A_00555"
     CDS             6626..6823
                     /locus_tag="AC162A_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009357.1"
                     /translation="MPLDPLAIGFAIVLIVAFVLIGSRFFPEDANILPWRSSARRPVV
                     REDDDARFNWGADERKDDVEP"
     gene            complement(6857..7300)
                     /locus_tag="AC162A_00560"
     CDS             complement(6857..7300)
                     /locus_tag="AC162A_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009358.1"
                     /translation="MAGCSAASTAGTSAPGAAAITAAAATPVAAATTPADASAPAATP
                     AGPSAAGGGAADFCAAWDEYQDARNADTSEETGAGFRAAATDIRATAPEEIKAAAGLL
                     ADVLDEFGQSMLAGGPQPETLGQGQSPERQKGVQDSIAWITTNCP"
     gene            complement(7511..7906)
                     /locus_tag="AC162A_00565"
     CDS             complement(7511..7906)
                     /locus_tag="AC162A_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR5009359.1"
                     /translation="MTRYVIDAGVAIRIAAGEIAVAAEHELLAPTLLRSQVLSTLHET
                     VARGELSEAEALERLGRLHRLSIRLLGDGVLRRRAWDLATKLGWASTYDAEYVALTQL
                     QADAFITQDEDLARSVQGLVTTASIDALR"
     gene            complement(7909..8256)
                     /locus_tag="AC162A_00570"
     CDS             complement(7909..8256)
                     /locus_tag="AC162A_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR5009360.1"
                     /translation="MSIERIDPGRRFAQAVAYGDTVYLAGHVASSASAGVFEQTVEIL
                     AAIDGHLAAAGTDKSKLLSANIWLADIATFDEMNRAWDAWVDMANLPVRATVESKLAG
                     PDYTVEIAVIAAR"
     gene            8343..9092
                     /locus_tag="AC162A_00575"
     CDS             8343..9092
                     /locus_tag="AC162A_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009361.1"
                     /translation="MREVPLTDFIRNRTLDAELGALLSLLIEARVPAIVAGPAGSDRA
                     GLRDALVAFGPADTQTIRLAAGDEDFAWMPEAVELGWRHEGGTGRGGPRANAATTVLL
                     ADLDDGPGGTWGERARIAIRALAVGYGMLATATGERLEDVLLGLSAPPVRAGDDELAR
                     LGLVLILGPAHVTAAHYLRPVSRDGHGHVQRLGPAVLATWDAANDRFEHFAWGITAEL
                     ADRVGIRPIELEREQARRAEQLSSVAATRGV"
     gene            9124..>9828
                     /locus_tag="AC162A_00580"
     CDS             9124..>9828
                     /locus_tag="AC162A_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR5009362.1"
                     /translation="MARSANASPPSDRPTTLGELRATGWQPRTVKEELRANLVDRLSA
                     GTPIFPGIVGYDETVIPSIENAILAGQDIVFLGERGQAKTRLARLLVGLLDELLPVVR
                     GGELNDDPFAPISAAARELVEAQGDTTPIDWLPRDRRYAEKLATPDITIADLIGEVDP
                     IRVAEGRYLSDELTLHYGLIPRANRGIFTLNELPDLAERIQVGLLNVLEERDVQIRGF
                     TIRLPLDLLVVASANPE"
BASE COUNT         1409 a         3467 c         3494 g         1458 t
ORIGIN      
        1 ccgaacacct cgtcgacgcc gtctgcggcg cgagcccggt tgacgacgtc ggtcaggacg
       61 acccaatcgt cgcgtcgccc aggcctgacg acgagccccg ggacggccgg ccacccgtcc
      121 acggggacct cgaccgggct cgcgggcgat tcagtgcgca tcacggtgtc tccatggtga
      181 agggcttgcg gtaggaggtg ccgctggtcg cgacccggaa gccgcacgat tcgtacagcg
      241 cataggcacg gttctgattg tcggtgtcca cgccgagata cgcgctcgat gcgccggccg
      301 cgcggaggcg ttgcaggctc tcggcgatgc aggcgcgcgc caggccgagg cggcggtgtc
      361 gcggatgggt cgtgacgccg tcgaggagcc cggtgaccga gccatccggc cgatcctcga
      421 gcacgttgtg cacgagcgcg gccggctcgt cgcccttcca ggcgacgacc acgaggtcca
      481 ggcggaagcg cgggtcctcg acccactcct cgtagttctg ctcatccgcc tcgtactggc
      541 cccagtgctc cgagaacccc tccacgctca tggcgaagac ggcgggcagc tccgcctcag
      601 tcggtgagcg caggacatag ccatcgggag ccgggacgtc cgggaggttc tcgagatcgg
      661 gccggagcat ctccgcgtcc cagcggacgg ccgtgtagcc gcgttccgtg agcgcggcgt
      721 tgagctcgtc atcgcggccg aacgcgtagt tggcgaacca ggcccggcgg tcggtggcgt
      781 tggctcgcgc gacctcgacg ctgtgccgct cggtccagtc gacgaggtcc gtcgcgatcc
      841 cggctcccca ggcgtcgggg gccaggacga tgacgttgtc gtagctgcgg tcgccgtcga
      901 ccaggtcgtg ccattcgacc cggccgtacc cgacggtggc gccgtcacgt tcgacgatca
      961 ggcagtcgac gagggggtcg gagttcacga ggtgggcgta gtgatggcgc atggactcga
     1021 ggtcgatcgg ctcgaggacc ccggccaagg cgcgcagccg cgcgttcgcc ctggccatag
     1081 cgtcgatgtc gtcgacgccg cggtagcggc ggtaggtgat gtccgagccc gccgggccct
     1141 cgggcaggcg ggcaatgggc gtggtcattg ttcgctcccg tacttgcgct tcatgtggac
     1201 ggccaccgat gcgctgatcg ccttccagcg acggcgctcg gcggcgcgga ggatcgggtc
     1261 gttcgcgcgg gcaacgtggg ccgcctcgcg ctccagcttg cgatggctcg caaacctcgc
     1321 cgcatcgagc cggccgtcgt cgagggccgc ccggacggcg cagccgggct cgccgtcgtg
     1381 gcggcagtcc cggaaccggc agcccgccgc gagctcgccg atgtcggcga aggcgttctc
     1441 gaggccgtcg gccgcacccg cgacgccgag cgagcggatg cctggggtgt cgatgagcag
     1501 cgcgcccgcc ggcaggcgga tgagctcgcg atgggtggtg gtgtggcggc cgcgggcgtc
     1561 gtcgtcgcgg acggcgctcg tccgctggcg ctcgtggccg agcagggcgt tgacgagcgt
     1621 ggacttgccc acgcccgatg acccgagcac gaccgctgtg cggccggggg gcagatgatg
     1681 gtcggccaga tccgcgacgc cgtcgccggt cagcgcggag atcgtccgca cctcgacgcc
     1741 cggagccacg gcttcggcgg ccacccgcaa cccgtcgggg tcgagggcga cgtcggcctt
     1801 gttgagcagg atcacggggg tggcgccgcc ggtccaggcg accgcgaggt agcgctcgag
     1861 gcgccgcagg ttgaagtcgc cgtcgaggct cgtgatcacg agcgcgacgt cgacgttggc
     1921 ggccaggatc tgctcgccgg ccaggttgcc ggcgtacccg tcggcgctgg tgttgcgccc
     1981 gaaggcggtc cggcggggaa gcacggcctg gatcacggcg gtgtcgccgt cgccgaggcc
     2041 ggccaccgcg acccagtcgc ccacggccgg caggtcgccg gcgcccaccg cttcgtggcg
     2101 caggcggccg gcgatcacgg catcgcgatc gcccgatggc gtggcgacga cccaggcgtt
     2161 gcggtgggcg gccacgaccc gggcgggctg ccagcctgcc gcgtcgaacg gcaggaaggc
     2221 ggcggcccag cccgggtccc agccaaggtc ctggagggcg ggtgaggcgg agacggccat
     2281 cggaaggtcc atctcagccc acccgcttca gcttgccggt gcgcttcgcg taccgttcgg
     2341 cccgcccgaa ggcccacaga ccggccggga tgagcacgat gcccatcacg atcaacggca
     2401 cgacgtcgta ccacagctcg gtcaccggga cgccgtcgag caggccggcg cggacgccgt
     2461 ccaggacgta cgtcgccggc gagaacttcg agagcacctg catccattcg ggcaggacgc
     2521 tgatcgggta gtacacgccc gacacgagca gcaggcacga ctgcaggacg aaggtcatct
     2581 gcgccccgcg ctccacgtac aggagcggca ggatcgcggc catcatcccg atgccgacga
     2641 tgctgaacga gccgagcagg acgaacacca gcgcggtggg cacgtcctcg acatgcaggg
     2701 tcaggtcgcg gaagaacacc atcatcacga tcaggatcac gccggtgtgc acgaggccga
     2761 agaccatcgc gtacaggcac gatcccagga gctgcgtcca gcgccggatc ggcgccatga
     2821 acgtgtactc cagggtgccc tcccagcgct ccacctggat cgtctcggcg atccagccga
     2881 acacgacgct gaggtagttc cagaagatgg cgccgatcat gagggtgagg agcaggcgcg
     2941 tatcgcccgc cgcggcgccg atcagcgtga tcgaaagcgc gccggcgacg gagtagacga
     3001 ggaacgcgat ctcccaaccc cagtaccggc gggtgaggaa gaagttgcgc tcgacgaagg
     3061 cgtacgacgc cttcagctcg tgcgtcagca gagtcaaggg actgtctcca gatcgagggt
     3121 tgggcccccg cccgccggca tcagccggtc agggcggggg agccgaggct ctcaccggcc
     3181 acccggcggc cgagtcggat gatcgactcg cccacctggg accggatcga gcgcgactgc
     3241 cttgcggcag cgcggtcggc gtcgcggaac gaccgctcga gcgactggcg ctcgttgatc
     3301 gccgcgagcc ggagctcgat tccgaacatt cgatggtcct ttcgagaggt gaggtgatgg
     3361 cggttggtgt ggccgttgtg cttggcggtc aacgtggtca tcgcggcacc tcctttcatg
     3421 tcagtcgttg tcgtcgtctt cctcgacgtc ctcgtcgagg gagcgaccgg tgaaggtcat
     3481 gaacacggct tcgagcgtcg gcggctgccc gtgttgctcg gcgacgagtc gcttgagccc
     3541 ctcgggcgtg tcctcggcga cggtcttccc cccgtcgagg atcgtgatcc gctcgcagag
     3601 gcgctccgcc tccgccaggt cgtgcgtcgt gaggacgatc gtcgcgtcgt gcgcggcgtt
     3661 gacctcctcg atgtacgcct ggacgtcgag cttgctgcgc gggtcgaggc ccgtcgtcgg
     3721 ctcgtccagc aggagcagcg tcgggctggt cagcagggct cgcgcgatcg cgaccttctg
     3781 ctgcatcccc cggctcatct gctccaccgg ccgaccgagg cgactctccg cgatgcccag
     3841 ccggtggagg atcgccgtcg cctcgcgctt cgcctgcttg ccgtcgaggc cgtacaggcg
     3901 ggccgcgaag aggaggttct ccatcgggct gagcttcttg aagaaggccg cgtcgaccga
     3961 aacccggttg atgagccgct tcacggcgag ctcgttgcgc tcgatgtcgt agccgaacac
     4021 ctcgaccttg ccctcgtcca gggtcagcaa ccccgagacg aggcggatga gggtcgactt
     4081 gccagagccg ttggcaccca ggaccccctg gatcgtgcct cgctccaggc ggagcgagac
     4141 gtcgtcgatc gcgaggaccg ggggcttgcc ccgacggacg ttgaagcgct tggtgacgtg
     4201 ctcgacgagg agtgccggcg ggcctgccgg tgtcggttgc gggaggacat tggcgtcccc
     4261 cgcgtccgga ccggccggga tccgcgcggc gggctcgagg ccggtctcgg tggccggcag
     4321 cgggctcgac gtcggatgag tcgtcttcgg ttcgggaacg agggtcgtcg ccactggact
     4381 ctcctgcgat gggttggctt gactcgagtg agtgagccgg tccgcgggag gtcccagaca
     4441 tgggacgagc cgcgggcccg gaaatccggg aggcctgcgg ctcgtttctt cgggcgaatc
     4501 agacgcctga aaaatgaata gagccgcggg cccggtggcc cacggctcgg tggtcgctcc
     4561 tggggcttac gccccgcgtc ctacctgctg gtgggcacac ctctcccgcc gacggcgcca
     4621 ctatcgggcg cgatcgagcg cgtcttggag aggatcatcg acaccatctg gcttagcgcc
     4681 tccggtaggg aagccgagcg acctgctcga ccgccgcgca gcatacgcgc gccgtcgccg
     4741 ccccgtcaac cctccagcgc cgggacaaac gacgtacctc ggtgacgcat ccgtcccggc
     4801 tcgcctccgt ctgtgcacga aacgctcgtt gcgaccatgc gactggatga ccgcccggtg
     4861 catggccccc atcggtggta cgaatcctcg ggccgggaac cgtcgtcgcg tcccgcgcgt
     4921 cgacgggtcc ggcagtcagc gagaggttcc ccgcttggtt tcgaaccgcc agcagagcgc
     4981 acggatcatg gcggcgctcg ccaccatcct gctcgtcgcc gcatgcgccg gggcctcgcc
     5041 tgggtccgga tcgacgtccg cgagcgcgac cctcccggcc ccaaccccgg tcgggtccgg
     5101 cgccgccgcc gaagactact ggctgcgtct gacgacctcc caggcgatcc cgccgctcga
     5161 tcgcttcgag gtcctgccat cgctgtggat caccggcgac gggcacgcgg tcgtgccgct
     5221 ggtcctgccg acgccctatc ccggcccact tgttcagggg ctgtccggca ggacggtctc
     5281 ggacgccggg cgcgcggcga tcgtgcagga agcgcgcgac ctcgggctgc tcggtgggca
     5341 gaccgacttc tcatccgacg ccccgctacc cggaggcgtc gtcgggcaga tcgagctcgt
     5401 cgtcgacggc acgagggtca cgctgaccgg ggacccgtcg gcgcacattg aatgtgtcac
     5461 cgcgccgtgc gacccgccgc ctggctcggc cgcggcgttc ggcgccttct ggaaccagct
     5521 cctcgacctg ccgtcgtggc tggggtccga cctcggccca ccgatgccgt acgacccgcc
     5581 cgcctacgcg ctcgtcgtcg gcccgccgcc ggatccggag ccgccgctca gcccgccgcc
     5641 ggccgactgg ccgcttgatc agccattggc gagtttcggc gctcgcgtgg ccaacgggac
     5701 ggcgcgatgc ggcacggtgt cgggtgctga cgccgacgcg ctacgcccgg cgctggacgc
     5761 cgccaatcaa ctcacgccct gggttcagga cccggagacg agcgcgacgt tcgggctgac
     5821 ggtccggccg atggtctccg gcgaggacgc ctgcaaggag tcgttcgggg tcggctgagc
     5881 ctgcgtcagg gcgcccggcg catcaccacc cgcggcgtgc ccgacgccgc cttcgaggtt
     5941 gacggcgagg ccacctcgaa cccggcacgc tcgaacatcg cggcggtccc ggtgtacacg
     6001 gatgcggacg agatccgggc gccgccggtc gcgaccgggt agccttcgag gacgccggca
     6061 ccgtgatccc gggcgtaccc gaccgctgcc tcgaggagcg ctctcgcgac gccgctgcga
     6121 cgcgatccct tcgcgacgac gaagcagttg atcacccaga cgtcctcgcc cggcagctgg
     6181 ggcagcgtcc gagaccgggc aagccgcccg aacccctccc gtggcccgag cccgacccag
     6241 cccacggcct tgccgtcgtc gatggccacc agcccgggcg acggcccacc gccttccatc
     6301 agggcccaca ggtcgtcacg gttgtcggcg gcggtcgtgt tgctccagtt cgagcccggc
     6361 ttgcgccaga actggcacca gcaccacttc ggatcgccgc cggccgagaa caactcggcc
     6421 agggacgccc agtgcccgga cgagagcgga acgatctcca cgcccgcatt gtggcctgcg
     6481 aggcgtcagg actcggcagg aatctcgaac tcgcgtgggc atcgagccgg gaacgactcg
     6541 gcgagcgcgg agccgcaggc gacgctaggc tgtcgggtga gtcgacgttg ccaggctcgc
     6601 accgagatcg gcgaggaggc aggcaatgcc cctggacccg ctggcaatcg gtttcgccat
     6661 cgtcctgatt gtggccttcg tcctgatcgg gtcgcgcttc tttcctgagg acgcgaacat
     6721 cctcccgtgg cgctcgtccg cacgccggcc ggtggtcagg gaggacgatg acgcacggtt
     6781 caactggggc gctgacgagc ggaaagacga cgtcgaaccc tgaccgtccc ggctcgagcc
     6841 tcagccagcg tggacgtcag gggcagttcg tggtgatcca ggcgatcgag tcctggacgc
     6901 ccttctggcg ctccggggac tgcccttggc cgagcgtctc gggttggggc ccgccggcca
     6961 gcatgctctg cccgaactcg tccagcacgt cggccaacaa cccggccgca gccttaatct
     7021 cctcgggtgc ggtcgcgcgt atgtccgtgg cggcggcccg gaagccggcg ccggtttctt
     7081 cggacgtgtc cgcgttgcgg gcgtcctggt attcgtccca ggcggcgcag aagtcggctg
     7141 cccctccgcc ggcggcggat gggcccgcgg gggtcgcagc gggcgcagaa gcgtcggccg
     7201 gcgtcgtcgc ggccgccact ggagttgccg ccgccgcagt tattgctgcc gctcccggcg
     7261 cgctggtccc ggcggtcgac gccgcggagc atcccgccaa gacgatcgtt ccaagaacgg
     7321 cggtgcacgc caggatggcg gccggcccgc tggagagctt ccgatgcaca aggactcctt
     7381 gcggcgcgcg cgcgctcgcc cggagggtcg tttggtctcc cggtgaggac cttccccgtg
     7441 tctccctccg gatcgacccg agtatgcgcg tggcgcctgc caaactcctg cccgcgccgc
     7501 gccaaccctg ctagcggagg gcgtcgatcg aggcggtcgt gaccaggcct tgcacgctcc
     7561 gtgcaaggtc ctcgtcctgg gttatgaacg cgtcggcctg gagctgggtg agcgcgacgt
     7621 actccgcgtc gtaggtcgac gcccagccga gcttggtcgc cagatcccat gcccgacgcc
     7681 gcagcacgcc atcgccaagg agccgtatgg acaaccgatg gagccttccg aggcgctcga
     7741 gcgcctcggc ctccgacagc tcgccccgcg cgacggtctc gtggagggtg gacaacacct
     7801 gcgagcggag cagcgtcggg gccagcagct cgtgctcggc cgccacggca atctcgccgg
     7861 ccgcgatccg gatcgcgacg cccgcatcga tgacgtacct ggtcatcgtc agcgggccgc
     7921 gatgaccgcg atctcgaccg tgtagtcggg gcccgccagc ttcgactcga cagtggcgcg
     7981 gaccggcagg ttggccatgt cgacccacgc gtcccaggca cggttcatct cgtcgaaggt
     8041 ggcgatgtcg gcgagccaga tgttcgccga gaggagcttc gacttgtcgg ttcccgcggc
     8101 tgcgaggtga ccgtcaatcg ccgcgaggat ctcgaccgtc tgctcgaaca cgccggcact
     8161 cgcgctgctc gcgacgtggc cggccaggta gaccgtgtcg ccgtaggcca cagcctgcgc
     8221 gaaccggcgc ccgggatcga tgcgttcgat gctcatgtgg ctcccctgtg ctgacgggtt
     8281 gggatgggtc cgatggtgcc acgacccttc gaggtcaggc agacgatgta gcctccgcct
     8341 cgatgcgcga agtgccactg accgacttca tccgaaaccg cacgctcgac gccgaactgg
     8401 gtgcgctcct gtcgctgctg atcgaggcac gcgtgccggc catagttgcg ggccccgcag
     8461 gatccgaccg cgccggcctc cgcgacgcac tggtcgcctt cgggccggcg gacacacaga
     8521 cgatcaggtt ggccgccgga gacgaggact tcgcgtggat gcccgaggcg gtcgagctgg
     8581 gctggcgaca tgaaggcgga acgggcaggg gaggaccgag agcgaacgcc gcgaccacgg
     8641 tcctgctcgc cgacctggac gacggccctg gcggcacctg gggcgagcgc gcgaggatcg
     8701 cgatccgggc gctcgccgtc ggctacggca tgctcgcgac ggcgaccggt gagcggctcg
     8761 aggacgtcct cctcggcctc tcggcccctc cggtcagggc cggggacgac gagctggcgc
     8821 gtctcggcct ggtgctgatc ctgggtccgg cgcacgtgac cgcggcgcac tatctccgac
     8881 cggtctcgcg cgacggccac ggccacgtcc agcgcctggg accggccgtc ctggcgacct
     8941 gggacgccgc caacgaccgg ttcgagcact tcgcctgggg catcacggcc gagttggcgg
     9001 accgggtcgg catccggccg atcgagctcg agcgcgagca ggcccggcgg gccgagcagc
     9061 tgagcagcgt ggcggcgacg cgaggcgttt gacgcgggtg ggaggccgtg ccacgctcag
     9121 atcgtggccc gctccgccaa cgcctccccg cccagcgacc gccccacgac cctcggggag
     9181 ctgcgtgcca ccggctggca gccgcgcacc gtcaaggagg agctacgggc caacctggtc
     9241 gaccgcctgt cggccggaac cccgatcttc ccgggcatcg tcggctacga cgagacggtc
     9301 atcccctcga tcgagaacgc gattctcgcc gggcaggaca tcgtgttcct gggcgagcgc
     9361 ggccaggcaa agacgaggct cgcacgcctc ctcgtcggcc tcctcgacga gttgctgccc
     9421 gtcgtccgcg gcggggagct caacgacgac ccgttcgcgc cgatcagcgc agccgcccgc
     9481 gagctggtcg aggcgcaggg cgacacgacc ccaatcgact ggttgccgcg cgaccgccgg
     9541 tatgccgaga agctcgccac ccctgacatc acgatcgccg acctgatcgg cgaggtcgat
     9601 ccgatccgtg tcgccgaagg ccgctacctg tccgacgagc tgaccctgca ctacgggctg
     9661 atcccgcgcg ccaaccgggg catcttcacc ctcaacgagc tgccggacct ggccgagcgg
     9721 atccaggtcg gcctgctcaa cgtcctcgag gagcgtgacg tccagatccg cgggttcacg
     9781 atccgcctgc cgctcgacct gctcgtcgtc gcgtccgcca acccggag
//
LOCUS       DBNHXO010000025         6796 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2981535, whole genome shotgun sequence.
ACCESSION   DBNHXO010000025 DBNHXO010000000
VERSION     DBNHXO010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2981535"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="AC162A_00585"
     CDS             <1..432
                     /locus_tag="AC162A_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009363.1"
                     /translation="EEQVEADVYLLTDEAPALLPAPTGDNGIRLDYSASASDQLLDDL
                     RSDRGMEWIPSSAWLTKVGVDSSAAGLSYDLAIDASGANAPSRVDAGLDLPGIGGGAE
                     TVVDGGRGIVAGRLLLALMVSLAGVAGILALTRYQPGRRSR"
     gene            443..868
                     /locus_tag="AC162A_00590"
     CDS             443..868
                     /locus_tag="AC162A_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HIR5009364.1"
                     /translation="MSPARVATSLGLGVVALLGAAVMALAVAREPEPIVVDISIHYSR
                     FLPAAIAVPAGRPVTFVIDNVDPIDHEWIVGDAAVHLRHRTGTEPSHGARPTEVSIDA
                     GTTRSTTVTFTEPGTLTYVCHLPGHEAYGMTGTVIVTAR"
     gene            complement(936..6767)
                     /locus_tag="AC162A_00595"
     CDS             complement(936..6767)
                     /locus_tag="AC162A_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR5009365.1"
                     /translation="MARRQLAVLAAFALLIVQAGGALAAPTAGASPGGGTASAPIAKG
                     LVAALNAESTDRFVVQFAAKADLDGAAKTKGFVKRGRAVLDALTTTAKQSQAAGLALA
                     KKSGAKARSYWLLNQLVVKGDAKLAKQFAKLKGVTRVRALRTYPLVKPVEMNAAVLAV
                     VGDPEWGVEKIRAPEAWAEGILGQGVVVANVDTGVDFTHPALVDHYRGNNGDGTFTND
                     YNWWDPTGICGDEPCDNAGHGTHTMGTMVGGDGPGPFTPDIGVAPAATWIAAKGCEEF
                     DCTEESLLSAGQWILAPTDSNGANPDPSKRPDIVNNSWGGGPGDEFYHATVNAWRAAG
                     IIPVFSSGNPGDACGDGGSPGDFNEVFSAGATDIDDNIADFSGRGPSGYGKVNPDISA
                     PGVDVVSSVPGDGYESFSGTSMAAPHTSGTIALLLSATPALIGAPNAYTATTDAIRAT
                     AVDRIDTTCGGDDDGDPNNVYGDGRIDAKAAVDLLATGGTLAGDVTDDDSGDPVSGAR
                     VTAAGTDRDFSVTADSDGHYEIFLAAGTYDVTADAFGYATGLEAGVEITTDNTTTVDF
                     ALTALPRFNLTGTVRASEDGSPLSGAAVAALGTPVAPAIADGSGDYTLNLPIGTYSVR
                     ASAGGCTESVVEDGVEIVDDDVDLDFALGRKLDTFGHGCRTVDLEWVDATTDTSLFGD
                     DFAGRLNLPFTFDFYGVGYNQVFISDNGYLTFGGADLFNAFPSPIPSESTPNGAVYAL
                     WRDLYVVGDGQISYATVGSPGNRAFVIEMNDVSVRGATSTVDIEIKLYEIGETVDILY
                     GNNDANPGDGRGSTIGIEDADGTDALEFSFSDSLVTANTAYRYEVVPTGIVGGTVTDA
                     NDDEPIAGATVSASPGLGSTTTAADGSYSLRLYPGDYDLTFSASGYVTETQGLSIADG
                     DELTRDVSLEAPVPTVDPDTVEVVLELGSDPVDAHVTLGNDGSAPLDWEAKERSGGST
                     PPVIEPTVNGHGVFTQGNKPAIQATRNGGGTALAHPRSYTWTAANPSADMSILVYADD
                     AVHAAPDTYVDQALQRLGLSYTAHYDADFDGFASDLESGSWDLVIFADEAWGPDFALF
                     DALNAYVVNGGRLIFGTYVVEFDPSHPLFETLGFSFSESVFDEPAPVYWWQPDHPIFT
                     FPEEAPEPSDQAAMGYGIYGQAGDPLAGAEALAGYTTPGPDDGLASLVIANEERTAFK
                     GWVDASNDADLDNDGVPDGVEFWEDAANGIASGFFTDVSWLSESPTDGTLDEGDTADV
                     TLTIGDPNLAPGEHKATVVFRTNAPKPRNVTVDVTLTVPLPGEWGALEGTVTDADTDE
                     PLADADVTLHSQWDGSPLDVSVTTDGDGAYSLLGPGGTWPLEFSKDGYTSVTRNQLVT
                     PATTTTGVDARLRQRVTHGRIKAGDLTFVMTKGNKDTGKITLTNKNGQIPLTFEVDEA
                     NIAGAPSLAGSATPRSIAKVAPLARDTRGANRPAVRVPPKLQSTGDILAAWPTEGLEV
                     PWAVGFTGNVWIGDFAIDGEPCGFAGGCTDTEFDVDGIPTGATIETPWAEGFGGDMAY
                     DAGRGLMWQVNVGGDNGIYGVDPADGSVVQTLTGSPWSDVDQRGLAYDPVADVFYIGG
                     WNEGIVYRVAGPSHTTPGETLSQCSPADGNISGLAWNGSFNLLWEATNSETDTIYLID
                     PATCDTIRAIDHPDPFFNGAGLDLDASGNLWMVSQASGLAFLVDAGLPLLSNVPWMTV
                     TPTSGSVAPDKKRTLTVTVNSKGLKPGLYRGAVVISTNDPEIGIAQVPVTLIVPKYRK
                     GINAGGRAYTDKAADIVYTKDRKFEPGGFGWVGSSSTQSTNDPIARTKRDPLYQDLRV
                     DMTGYKFKVPNGFYVVDLSFAELHYRKAGGRQFAIEIEGQTMVSGFDVVDEAGGRKTA
                     VDETYLVEVTDGRLNINFTAPRAHKPIVNALLVTEMPPGAFGF"
BASE COUNT          951 a         2251 c         2491 g         1103 t
ORIGIN      
        1 gaggagcaag tcgaggccga cgtgtacctg ctcaccgacg aggcccccgc gctgctcccg
       61 gcgccaaccg gcgacaacgg catccgcctc gactacagcg cttcggcgag cgaccagctc
      121 ctcgacgacc tgcgcagcga ccgcgggatg gagtggatcc cgtcctcggc ctggctgacc
      181 aaggtcggcg tcgactcgtc ggccgccggg ctgtcctacg acctggcgat cgatgcgagc
      241 ggcgccaacg ccccgtcgcg cgtcgatgcc ggcctcgacc tgccgggcat cggtggtgga
      301 gcggagacgg tcgtcgacgg cgggcgcggc atcgtggcgg gccggctgct gctcgcgctg
      361 atggtgtccc tggccggggt cgcggggatc ctcgcgctga cccgctacca gccgggccgc
      421 cggtctcgct gaggcgatca gcgtgtcgcc cgcacgggtc gccacctcgc tcggtctcgg
      481 cgtcgtcgcg ctgctcggcg cggcggttat ggcgcttgcg gtcgcccgcg agccggagcc
      541 gatcgtcgtc gacatctcga tccactactc gcgcttcctg ccggcggcga tcgccgtgcc
      601 ggcaggacgg ccggtgacgt tcgtcatcga caacgtcgat ccgatcgacc acgagtggat
      661 cgtcggcgac gcggcggtcc acctccgcca tcggaccgga acggaaccgt cacacggcgc
      721 ccggccgacc gaggtcagca tcgatgccgg cacgacgcgc tcgaccacgg tcacgtttac
      781 cgagccgggc acgctgacct acgtctgcca tctcccgggc cacgaggcgt acggcatgac
      841 ggggacggtg atcgtcaccg cccgctagca cccgccagac gagcgaacgc cccgccggtg
      901 gcggggcgtt cgtgtgcgag tgactgaacc ggaggtcaga acccgaaggc gcccggtggc
      961 atctcggtca cgagcagcgc gttcacgatc ggcttgtggg cgcgcggtgc cgtgaagttg
     1021 atgttgagcc tgccgtcggt gacctccacg aggtaggtct cgtcgacggc ggtcttgcgg
     1081 ccgccggcct catcgacgac gtcgaagccc gacaccatgg tctggccctc gatctcgatg
     1141 gcgaactggc gcccgcccgc cttcctgtag tggagctctg cgaacgaaag gtcgaccacg
     1201 tagaagccgt tggggacctt gaacttgtag ccggtcatgt cgacgcgcag gtcctggtac
     1261 agcgggtcac gcttggtgcg cgcgatcgga tcgttggtgg attgcgtcga gctcgagccg
     1321 acccagccga agccgccggg ctcgaacttc cggtcctttg tgtagacgat gtcggcggcc
     1381 ttgtccgtgt aggccctgcc gccggcgttg atgcccttcc ggtacttggg gacgatcagg
     1441 gtcacgggca cctgggcgat gccgatctcg ggatcgttcg tgctgatcac gacggcaccg
     1501 cggtacaggc cgggcttgag gcccttgctg ttgacggtga ccgtgagggt ccgcttcttg
     1561 tccggggcga ccgagccgct cgtgggcgtc acggtcatcc acgggacatt gctgagcagc
     1621 ggaagtccgg cgtccacgag gaacgccagg ccgcttgcct gcgagaccat ccacaggttg
     1681 ccggaggcgt cgaggtcgag gcccgcgccg ttgaagaacg gatccggatg gtcgatcgcc
     1741 cggatcgtgt cgcaggtcgc cggatcgatc aggtagatcg tgtccgtctc gctgttggtc
     1801 gcctcccaca acaggttgaa ggagccgttc caggcgaggc ccgagatgtt cccatcggcg
     1861 gggctgcact ggctgagcgt ctcgcccggc gtcgtatgcg acgggccggc gacgcggtac
     1921 acgatgccct cgttccagcc accgatgtag aacacgtcgg cgaccgggtc gtaggcgagg
     1981 cctcgctggt cgacgtccga ccacggcgac cccgtgaggg tctgaacgac ggacccgtcg
     2041 gcggggtcga cgccgtagat gccgttgtcg ccgccgacgt tgacctgcca catgaggcct
     2101 cggccggcgt cgtaggccat gtcgccgccg aagccctcgg cccacggcgt ctcgatggtc
     2161 gccccggtcg ggatgccgtc aacgtcgaac tcggtgtcgg tgcacccgcc ggcgaatccg
     2221 cacggctcac cgtcgatggc gaagtcgccg atccacacgt tgccggtgaa gccgacggcc
     2281 cacggcacct cgaggccctc cgtcggccag gcggcgagga tgtcgccggt cgactggagc
     2341 ttcggcggca cgcggacggc gggccggttg gcaccacgcg tatcccgcgc gagcggcgcg
     2401 accttggcga tcgacctggg cgtcgcgctg ccggcgaggc ttggcgcgcc ggcgatgttg
     2461 gcctcatcga cctcgaacgt gagggggatt tggccattct tgttggtcag cgtgatcttg
     2521 ccggtgtcct tgttgccctt ggtcatgacg aaggtcaggt caccggcctt gatccgacca
     2581 tgcgtgacgc gctggcgcag ccgcgcgtcg acacccgtcg tggtcgtggc gggggtcacc
     2641 agctggttgc gggtgaccga ggtgtagccg tccttgctga actcgagcgg ccatgtcccg
     2701 cccggaccca gcagcgaata ggctccgtca ccgtcggtgg tgacggacac gtcgaggggc
     2761 gagccgtccc actggctgtg gagggtgacg tcggcatcgg cgagcggttc gtcggtgtcc
     2821 gcgtcggtca cggtgccctc gagggcaccc cactcgccgg ggagcggcac cgtcagcgtc
     2881 acgtcgaccg tgacgttgcg cggcttgggt gcgttggtcc ggaagacgac ggtggccttg
     2941 tgctcgccgg gcgcgaggtt cgggtcgccg atcgtgaggg tcacgtccgc tgtgtcgccc
     3001 tcgtcgagcg tcccgtccgt cgggctctcc gacagccacg agacgtccgt gaagaacccg
     3061 gaggcgatgc cgttggcggc gtcctcccag aactcgacgc cgtcggggac gccgtcgttg
     3121 tcgagatctg cgtcgttgct ggcgtcgacc cagcccttga acgcggtgcg ctcttcgttg
     3181 gcgatgacga gcgacgccag gccgtcgtca gggcccgggg tcgtgtagcc ggccagcgcc
     3241 tcggcaccgg cgagcggatc gcccgcctgg ccgtagatgc cgtagcccat cgccgcctga
     3301 tcggacggct cgggggcctc ctcggggaag gtgaagatcg gatggtccgg ctgccaccag
     3361 tacacgggcg ccggctcgtc gaacacgctc tcggagaacg agaacccgag cgtctcgaac
     3421 agcggatggc tgggatcgaa ctcgaccacg taggtcccga agatcagccg gccgccgttg
     3481 acgacgtagg cgttgagcgc atcgaacagc gcgaagtcgg ggccccaggc ctcgtcggcg
     3541 aagatcacca ggtcccagct gcccgactcg aggtcactcg cgaagccgtc gaagtcggcg
     3601 tcgtagtggg ccgtgtacga caggccgagg cgctggagcg cctggtcgac gtacgtgtcc
     3661 ggcgccgcgt ggacggcgtc gtccgcgtag acgaggatcg acatgtcggc cgaggggttg
     3721 gcggcggtcc acgtgtatga gcgggggtgc gcaagcgccg tcccaccgcc gttgcgggtc
     3781 gcctggatcg cgggcttgtt gccctgggtg aagacgccgt ggccattgac ggtcggttcg
     3841 atgaccggag gcgtcgagcc gccgctgcgt tccttggcct cccagtcgag cggtgcgctg
     3901 ccgtcgttgc cgagcgtcac gtgcgcgtcc accgggtcgg aaccgagttc gagcacgacc
     3961 tcgacggtgt cggggtcgac ggtcgggacg ggcgcctcga gcgagacgtc gcgagtcagc
     4021 tcgtcgccat cggcgatcga gaggccctgg gtctccgtca cgtagcccga ggcggagaag
     4081 gtcaggtcgt agtcacccgg atagagccgc agcgagtagc tgccgtcggc ggccgtcgtc
     4141 gtcgacccga gtcccgggga tgccgagacg gtcgcgccgg cgatcggctc gtcgtcgttg
     4201 gcatccgtga cggtgccgcc gacgatgccc gtgggcacga cctcgtagcg ataggcggtg
     4261 ttggcggtga ccagcgagtc ggagaacgag aactcgaggg catcggtgcc gtctgcgtcc
     4321 tcgatgccga tcgtggagcc gcggccgtca ccggggttgg cgtcgttgtt gccgtacagg
     4381 atgtcgacgg tctcgccgat ctcgtacagc ttgatctcga tgtcgacggt tgacgtcgcg
     4441 ccccggaccg agacgtcgtt catctcgatc acgaaggctc ggttgcccgg cgagccgacg
     4501 gtcgcgtacg agatctggcc atcgccgacc acgtacaggt cacgccacag ggcatagacc
     4561 gcgccgttgg gcgtcgactc cgaggggatc ggactcggga acgcgttgaa caggtctgcg
     4621 ccgccgaacg tgaggtagcc gttgtcggag atgaagacct ggttgtagcc gacgccgtag
     4681 aagtcgaacg tgaatggcag gttgagccgg ccggcgaagt catcgccgaa cagggaagtg
     4741 tccgtcgtcg catcgaccca ctcgaggtcg accgtgcggc agccgtggcc gaatgtgtcg
     4801 agcttgcgcc cgagcgcgaa gtccaggtcg acgtcatcgt cgacgatctc cacgccgtcc
     4861 tcgacgacgc tctccgtgca gccgcccgcg gaggcgcgca ccgagtacgt cccgatgggc
     4921 aggttcaggg tgtagtcgcc cgagccgtcg gcgatcgccg gggcgacggg cgtcccgagc
     4981 gcggccaccg cggcgcccga gaggggggag ccgtcctccg aggcccgaac ggtgccggtg
     5041 aggttgaacc gcggcagcgc cgtgagcgcg aagtcgacgg tggtcgtgtt gtcggtcgtg
     5101 atctcgacgc cggcctcgag accggtcgcg tagccgaacg cgtcggccgt cacgtcgtag
     5161 gtgccggccg cgaggaagat ctcatagtgg ccgtcgctgt ccgcggtgac gctgaagtcg
     5221 cgatccgttc cggccgccgt gacgcgcgcg cccgagaccg ggtcgccgga atcgtcgtcg
     5281 gtgacgtcac cggccagcgt gccgccggtg gcgaggagat cgaccgctgc cttggcgtcg
     5341 atgcggccgt cgccgtagac gttgttcggg tcgccgtcgt cgtcgccgcc gcaggtcgtg
     5401 tcgatgcggt cgacggccgt cgcgcggatc gcgtcggtcg tggcggtgta ggcgttcgga
     5461 gccccgatca gggcaggggt ggccgacagc aggagggcga tggtgcccga cgtgtgcggg
     5521 gccgccatgg aggtgccgga gaatgactcg tagccgtcgc ccgggacgct cgagacgacg
     5581 tccacgccag gggcgctgat gtcgggattg accttgccgt agccggacgg gccgcgaccc
     5641 gagaagtcgg cgatgttgtc gtcgatgtcg gtcgcgccgg cgctgaagac ctcgttgaag
     5701 tcgcccggcg aaccgccgtc accgcaggcg tcgcccgggt tgccggacga gaacaccggg
     5761 atgatcccgg ccgcgcgcca ggcattgacg gtcgcgtggt agaactcgtc gccggggccg
     5821 ccgccccacg agttgttgac gatgtcgggg cgcttcgacg ggtcggggtt ggcgccgttg
     5881 ctgtcggtcg gggccaggat ccactggccg gccgacagga gcgactcctc ggtgcagtcg
     5941 aactcctcgc agcccttggc cgcgatccac gtggcggccg gagcgacgcc gatgtccggc
     6001 gtgaacgggc cggggccgtc gccgccgacc atcgtgccca tcgtgtgggt gccgtgtccc
     6061 gcgttgtcgc acggctcgtc gccgcagatg ccggtcgggt cccaccagtt gtagtcgttg
     6121 gtgaaggtgc cgtcgccgtt gttgccgcgg tagtggtcga cgagggccgg gtgcgtgaag
     6181 tcgacgcccg tgtcgacgtt cgcgaccacg acgccctggc cgaggatgcc ctcggcccag
     6241 gcctcgggtg cacggatctt ctcgacgccc cactcgggat cgccgaccac cgcgaggacc
     6301 gcggcgttca tctcgacggg cttgaccagg ggatacgtcc gcagcgctcg gacgcgggtg
     6361 acgcccttga gcttggcgaa ctgcttcgcg agcttcgcgt cgcccttgac gaccagctgg
     6421 ttgagcagcc agtagctccg ggccttggcg ccggacttct tcgccagcgc gagaccggcc
     6481 gcctgcgact gcttcgccgt cgtcgtgagc gcatcgagca cggcgcgacc gcgcttgaca
     6541 aagcccttgg tcttcgccgc gccgtcgagg tcggccttgg cggcgaactg gacgacgaag
     6601 cgatcagtcg actcggcgtt gagcgccgcg acgaggccct tcgcgatcgg tgccgaggcg
     6661 gtgccgccgc ctggcgacgc accggcggtg ggcgcggcga gcgccccacc tgcctggacg
     6721 atcaggagcg cgaatgcggc caagaccgcg agctggcgac gcgccacggc gagaccctcc
     6781 tcgaaccgcc cggaca
//
LOCUS       DBNHXO010000026         1740 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1196025, whole genome shotgun sequence.
ACCESSION   DBNHXO010000026 DBNHXO010000000
VERSION     DBNHXO010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1196025"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1115)
                     /locus_tag="AC162A_00600"
     CDS             complement(144..1115)
                     /locus_tag="AC162A_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR5009366.1"
                     /translation="MTTQERDLDGLVAGVDLGGTKIQTIVTHGDEIVGQARVPTPEDG
                     AEAIVRSVADTIHVAVDASGADPSSLRSVGVGAPGRAHEGVVSRSPNIPGMHEPFPLG
                     PQLSEALAGVPVHVDNDVSVAMRGELERGAGRPFSSMLGVFVGTGVGGGIVMDRHVWR
                     GRGAAAEIGHMIVKPDGRPCGCGGHGHLESYAGRASMQAKAERLAAEGHHTDLFHLME
                     KHGRDRLTSGIIERALEHGDHMTKRLMEQAVWALGIAIASAQNLLDVEAVIIGGGLAE
                     RFGTPWVDEIAAQMQPLLFSPDQPPVVLPAELGDLSGAMGAVVLAGG"
     gene            1193..>1740
                     /locus_tag="AC162A_00605"
     CDS             1193..>1740
                     /locus_tag="AC162A_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HIR5009367.1"
                     /translation="MPLPEHTTLNWGPWYRRSPFFEATLRAGCSAYDVYQHMYHPNTY
                     GDPVEEYWALVNDVTLWDVSVERIVEITGPDASAFTNLLTCRDLTNCAVKQGKYMLVT
                     AEDGGIVNDPVLLRVEENRWWLALADSDAGLYAMGVAVNSGLDVQVGHPDVYPVQVQG
                     PKAKETMRDLFGDWILDMKYYW"
BASE COUNT          267 a          603 c          608 g          262 t
ORIGIN      
        1 gcgacggcct cgatcgcctc gagctgcccc ggccggaagg acgcgtctgg gccgagcatc
       61 tcgtgcaggt gagaggaggc ggagctgccg tcgttcacgc cagcaccgta ctcgcgaccg
      121 gtgacgagga gacgcggatc gggttagccg ccggcgagca ccacggcgcc catcgcgccg
      181 gagaggtcgc cgagctcggc gggcagcacc acgggcggct ggtcgggaga gaacaggagc
      241 ggttgcatct gcgccgcgat ctcgtcgacc cacggcgtgc cgaaccgctc cgcaaggccg
      301 ccgccgatga tcacggcctc gacgtcgagc aggttctgcg ccgaggcgat ggcgatcccg
      361 agcgcccaca ccgcctgttc catcagccgc ttcgtcatgt ggtcgccgtg ctcgagcgcg
      421 cgttcgatga tgcccgaggt caagcggtcg cggccgtgtt tctccatgag atggaacagg
      481 tccgtgtggt ggccttcggc ggccaggcgc tcggccttgg cctgcatgct cgcgcggccc
      541 gcgtaggact ccagatgacc gtggccgccg cacccgcacg gccggccgtc aggcttgacg
      601 atcatgtgcc cgatctccgc ggcggccccg cgaccgcgcc agacgtggcg gtccatgacg
      661 atgccgcccc ccacgccggt gcccacgaag acgccgagca tgctggagaa cgggcggccg
      721 gcgccgcgct cgagctcgcc cctcatcgcg acgctgacgt cgttgtcgac atggaccggt
      781 acgccggcga gcgcctcgga gagttggggc cccagaggga acggctcgtg catacccggg
      841 atgttggggc tccgtgagac gacgccttcg tgcgcgcggc ccggcgcgcc gacgcccacc
      901 gatcggaggg aggaggggtc cgctcccgac gcatcgacgg cgacgtggat cgtgtcggcg
      961 accgacctga cgatcgcctc ggcaccgtcc tctggtgtgg gcacgcgcgc ctgtcccacg
     1021 atctcgtcgc cgtgcgtcac gatcgtctgg atcttcgtgc cgcccaggtc gacgccagcg
     1081 acgagtccgt cgaggtccct ctcctgcgtc gtcatcgggt cagcgtagtg ggtggcgacg
     1141 cggcgcccta gactccggcc accgcgaagg accacgacga ggaggacacc tgatgccgct
     1201 gccagagcac accacgctga actggggtcc ctggtaccgc aggtcgccgt tcttcgaggc
     1261 gacgcttcgc gccggctgct ccgcctacga cgtctatcag cacatgtacc accccaacac
     1321 ctacggcgat ccggtcgagg agtactgggc gctcgtgaac gacgtgaccc tgtgggacgt
     1381 gagcgtcgag cgcatcgtcg agatcaccgg gcccgacgcg agcgcgttca cgaacctgct
     1441 cacgtgccgc gacctcacga actgtgccgt gaagcagggc aagtacatgc tcgtcacggc
     1501 cgaggacggc ggcatcgtca acgacccggt gctgctgcgc gtcgaggaga accgctggtg
     1561 gctcgcgctc gccgattccg acgccggcct gtatgcgatg ggggtcgcgg tcaactccgg
     1621 gctcgacgtg caggtcggtc accccgacgt ctatccggtg caggtgcaag gcccgaaggc
     1681 caaggagacg atgcgcgacc tgttcggcga ctggatcttg gacatgaagt actactggtg
//
LOCUS       DBNHXO010000027         2128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_600506, whole genome shotgun sequence.
ACCESSION   DBNHXO010000027 DBNHXO010000000
VERSION     DBNHXO010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_600506"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /gene="ftsZ"
                     /locus_tag="AC162A_00610"
     CDS             complement(<1..1077)
                     /gene="ftsZ"
                     /locus_tag="AC162A_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019636227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR5009368.1"
                     /translation="MMPLRSDSENFALIRVIGVGGGGSNAVNRMIRAEMMGVEFIACN
                     TDAQALLQSDAPHKIRIGDKITRGLGAGGDSSIGQRAAEEDRDKIADALADSDMVFIT
                     AGLGGGTGSGAAPVVAEMAKEQGALTIGVVTKPFSFEGSRRKLVAEKASEDLKAKVDT
                     LITIPNDRLRDVVQKNTSIVDAFRVVDDVLRQGVAGISDIITVPGLINLDFADVRAIM
                     KDAGSALMGIGRASGENRALEAARMAVASPLLEINIAGAQGILFNITGSSNLTLWEVT
                     EAAEEIRAAADPEANIIFGTSFNERLGEEIMITVIATGFDGRRRPDASRAQQPERVSA
                     GVARPAAAAASATAGGTVPARDFLG"
     gene            complement(1314..1727)
                     /locus_tag="AC162A_00615"
     CDS             complement(1314..1727)
                     /locus_tag="AC162A_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HIR5009369.1"
                     /translation="MQSIQLEVRTGRDRGFTDLTPACARFAGEASQGGDGLLSVFVPH
                     ATAGVVILELGAGSDVDAVATLDRLLPRDDRWTHRHGSPGHGADHVLPLLAAPSITVP
                     VIGGRLALGTWQSIALLDPNEDNASRRIRLSFLGG"
BASE COUNT          270 a          726 c          798 g          334 t
ORIGIN      
        1 cccgaggaag tcgcgggccg gcacggtgcc gcccgcggtc gcgctcgccg ccgcggcggc
       61 cgggcgggcg acgcccgcgg aaacgcgctc cggctgctgg gcgcggctcg cgtccgggcg
      121 gcgacggccg tcgaacccgg tcgcgatgac cgtgatcatg atctcctcgc cgagccgctc
      181 gttgaagctc gtcccgaaga tgatgttcgc ctcgggatcg gccgcagccc ggatctcctc
      241 ggcggcctcg gtcacctccc acagggtgag gttcgaggag ccggtgatgt tgaacaggat
      301 cccctgcgcg ccggcgatgt tgatctcgag cagcgggctc gcgacggcca tgcgggcggc
      361 ctcgagggcg cggttctcgc cgctcgcccg gccgatgccc atgagcgccg aaccggcgtc
      421 cttcatgatc gcgcgcacgt cggcgaagtc gaggttgatc aggcccggca ccgtgatgat
      481 gtcgctgatg cccgcgacgc cctggcggag gacgtcgtcg acgacccgga acgcgtccac
      541 gatcgacgtg ttcttctgga cgacgtcgcg cagccggtcg ttggggatcg tgatcagcgt
      601 gtcgaccttg gccttgagat cctcggaggc cttctcggcg accagcttgc gccggctgcc
      661 ctcgaagctg aacggcttgg tgacgacgcc gatggtgagc gcgccctgct ccttcgccat
      721 ctcggccacg accggcgccg cgccggagcc ggtcccaccg ccgaggcccg cggtgatgaa
      781 caccatgtcc gagtcggcga gcgcgtccgc gatcttgtcg cggtcctcct cggcggcccg
      841 ctggccgatc gacgagtcgc cgccggcgcc aaggccgcgg gtgatcttgt cgccgatgcg
      901 gatcttgtgc ggggcgtccg actggaggag cgcctgggcg tccgtgttgc aggcgatgaa
      961 ctcgacgccc atcatctcgg cgcgaatcat ccggttgacg gcattgctgc caccaccgcc
     1021 gacgccgatg acgcggatca gggcgaagtt ctctgaatcg gaacgcagcg gcatcagcgc
     1081 tctcctggat gttcgagtgt ggcggcgggc gatcgctgtc gctcgtcgtg ctgggggggc
     1141 gcgccggtcc cgggctccgg cttcgcgacg ggtctgcagg gttagggcgg gagtataccc
     1201 acggcatgtg cgtgcctgtc cacgggtcgg tcgcggggcg tcgtgcgtgg gcgccggttc
     1261 gccggtgccg acgggcaccg cggatcggtg gggtgcggtg cccttggtga cggtcagccg
     1321 ccgaggaagg agaggcggat gcgccggctg gcgttgtcct cgttggggtc caggagcgcg
     1381 atcgactgcc aggtcccgag ggccagccgg ccgccgatga cgggcacggt gatcgagggc
     1441 gcggcgagca gcggcaggac gtggtccgcc ccgtggcccg gggatccgtg gcgatgcgtc
     1501 cagcggtcgt cccgcggcag cagccggtcg agtgtcgcga ccgcgtcgac gtcggagccg
     1561 gcgcccagct cgaggatcac gacgccggcc gtcgcgtggg gcacgaacac cgacagcagc
     1621 ccatcgccgc cctgtgatgc ctcgccggcg aagcgcgcgc aagccggcgt caggtcggtg
     1681 aagccgcggt cccgcccggt gcggacctcg agctggatcg attgcatctc gacgatcctt
     1741 ctgtggtgtg gtctgcggat ttgtcgcgcg gcccgtcagt ggcggaacgc gagacgttca
     1801 ctggagccgt tccagtcgtc cgggatccgc catctggaac gtcccgcgtt caggttggcc
     1861 cccgcacggc cgaatggctc gcccgacgtt ccagcccagc ggtcctgtga tggcccctgc
     1921 gctgcagtga acgtatggcg ttccaggccg gcgggcgtgc ctggcggtac gcgcgctaag
     1981 ggaagatcga gcggatcgcg tcccggatgc ggccgaagcc gccgccgccc ggcgccgact
     2041 cgtagcgcag cggctcgccc tgggccacct ggatcgcccc ccagcggagc aggccgacgg
     2101 ccgtcgagta ggacgggtcc tggatcgt
//
LOCUS       DBNHXO010000028         4634 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1395293, whole genome shotgun sequence.
ACCESSION   DBNHXO010000028 DBNHXO010000000
VERSION     DBNHXO010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1395293"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="AC162A_00620"
     CDS             <1..1184
                     /locus_tag="AC162A_00620"
                     /inference="COORDINATES: protein motif:HMM:NF021134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HIR5009370.1"
                     /translation="ASSARRGRPLNADSLGPWVVAGVVAWLGVAWIGWTLWQGTPPRA
                     GFDLALLLDGARSVLAGASPYDAAMLAGASPDAVDLFYSYPPPVAQAMAVVSWLPNGV
                     VLLLWGIGATLGFGLVAARLALLDARPAVPMAIRAIAVAPLVLPFAIAVLFGNLDAWY
                     PLAYGALLLAALPGASRRTQVAGGVAVALVSIAKLHPAPLLLWIALRAWQARGGPMGR
                     VLLAAVVTGVAVLAVSLVVGGVQPWLDYVAVVRAAAGSALVDARNAAPVSLIGQLTGI
                     EGSVLRGLQLLIVAGVVVVTVVAALRVRDPLFSLALAIAASLVTLPVTWYHYPVALMP
                     IGAALAIWRPASRRWVAAAIVLADVAIVWLPILWVAVAVIVVAAAVTDGEPATAEPQA
                     T"
     gene            1259..2206
                     /locus_tag="AC162A_00625"
     CDS             1259..2206
                     /locus_tag="AC162A_00625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HIR5009371.1"
                     /translation="MAIATVNAIFGIGAAIILIPLTYGSDAAIYQRGAQGIHDGFYAK
                     DFFYSPLTGILATPLTWVPLPVAALAMALIGLGILTLGVARETERLPTVDRVLVFVAA
                     LGFVPVTYEIITGQVTMLIAAALYPVRERDGFARGIGVGLVLALFAKPMLLPVLVWML
                     LWRRQALVGTIGAAAAVTVLGVVLLGTGIYGAWIDAVVGTGKIVRPGNMALTDLAPPA
                     VVLGLSVIAIALAGWAIIRDERRGFVAALTAAMLVAPFTLMYQVSILLLAVRPALAVV
                     PRATRILALVANPAVLAVFIPWAAAVLVAVTPFSRRRPE"
     gene            2203..4497
                     /locus_tag="AC162A_00630"
     CDS             2203..4497
                     /locus_tag="AC162A_00630"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR5009372.1"
                     /translation="MTAAATAPAPRLSLAAALALIGVALGATLLVLGLGSWPGTYYSP
                     IRVASIVVAFAGLVIWLVIALVRPEHRPASGLAGAFAAALFAFAISAINAQNVRLAFD
                     FLGYAVLLTGLYLLFVRVWTSHAIALRLEALLALACLLGGVLFIAAMVNLWATMWSAI
                     GAIVMPPLRFGSEGLWTGTPNAMAAFQVLLYCGVVTSLLARGRRGRIVAVALGIVVVV
                     DVAISGSRGAWLGVALAVVVTGTVWLATTDRDRLRGTWSRLWSGRSRVFVIVGAVVAA
                     LAASAAVVVALPRLSEGGGDLRSDFISVSIRMFQAAPLTGQGPGSWGPERLAYTDASQ
                     IDYYVPHAHNVPAQTVAEFGLVGIVAGIIVVALVLRLVAHGLRGRSPAERGLALAALF
                     ACVYITGQQLVDAWIHQPAILFALAVPIARLDAALLRAETAPYPWTKRLLSVVLLVAV
                     VVGTGTALWPEPAAQLGLEAVGASEKGDWQRALDESTAAVTADPRMPPYQLMRGLSAA
                     HLGDMQTAREAFAAATADDLPASWINLAAVQLRLGDPVGAAASLDRGTRLGIQQPVVA
                     TAAAGLYHELGDDDEAARMLAAAFGAVPSLSADAMWRDPAWAPVAQQAVDAAVAGDDR
                     TAAMRVALESGRFGAARAIAHEIDPDDSDLAQTTVAAWAGDQGAFRKLHEAARADPVN
                     APLVEMCRRVARVHDPSLSTPGWACDGGWYFGAYPVATIGSGPLEASLPGANEYWQGL
                     YVYRRPGPADLLVPWVLHIQSTHA"
BASE COUNT          474 a         1715 c         1683 g          762 t
ORIGIN      
        1 acgcgtcctc ggcccgccgc ggacgcccgc tcaacgccga cagcctgggc ccgtgggtcg
       61 tggctggcgt ggtcgcgtgg ctgggcgtcg cctggatcgg ctggacgctg tggcagggca
      121 cgccgccgcg ggccggattc gacctcgcgc tactgctcga cggcgcgcgg agcgtgctcg
      181 cgggcgcgag cccgtacgac gcggcgatgc tggccggcgc gtcgccggac gccgtggacc
      241 tgttctattc ctacccgccg ccggtcgccc aggcgatggc tgttgtctcg tggttgccga
      301 acggcgtcgt gctcctcctg tggggcatcg gggccacgct gggcttcggg ctggtcgcgg
      361 cacggctcgc cctgctcgac gcccgtccgg ccgtcccgat ggccatccgg gccatcgccg
      421 tcgccccgct cgtactcccg ttcgccatcg ccgtgctgtt cggcaacctc gacgcctggt
      481 acccgctggc gtacggggcg ctgctgctgg ccgcgctgcc cggcgcgtcc cggcggaccc
      541 aggtggccgg cggcgtggcc gtcgcgctcg tctcgatcgc aaagctccac ccggcgccgc
      601 tgctgctgtg gatcgcgctg cgcgcctggc aggcccgcgg cgggccgatg ggccgggtgc
      661 tgctcgcggc cgtcgtcacg ggcgtcgccg tgcttgcggt cagcctcgtc gtcggcggcg
      721 tgcagccgtg gctcgactac gtggcggtcg tccgcgccgc cgccggctcc gccctcgtgg
      781 atgcgcggaa cgccgcgccg gtctcgctga tcggtcagct gaccgggatc gagggctcgg
      841 tgctacgcgg gctccagctc ctcatcgtcg ccggcgtcgt cgtcgtgacg gtggtcgccg
      901 ccctgcgcgt gcgcgacccg ctgttcagcc tggcactcgc gatcgccgcc tcgctggtga
      961 cgctaccggt gacctggtac cactatccgg tcgcgctgat gccgatcggc gccgcgctcg
     1021 ccatctggcg gccggcgtcg cgccggtggg tcgccgccgc catcgtcctc gccgatgtgg
     1081 cgatcgtgtg gctgccgatc ctgtgggtcg cggtcgcggt catcgtggtc gcggccgcgg
     1141 tcaccgacgg cgaaccggcc acggccgagc cgcaggcgac atgaccgcca cccccggact
     1201 cggctcgttc gccgcctcgc cgcggctacg ggctctcaca gcggacaggc cgtacctcgt
     1261 ggctatcgcc accgtcaacg cgatcttcgg gatcggcgcc gcgatcatcc tgatcccgct
     1321 cacctacggc agcgatgcgg cgatctacca gcgtggcgcg caggggatcc acgacgggtt
     1381 ctacgcgaag gacttcttct attcgccgct gacaggcatc ctcgcgacgc cgctgacgtg
     1441 ggtcccgctg cccgtcgccg ccctcgcgat ggccctgatc ggcctcggga tcctcaccct
     1501 cggcgtcgcg cgggagacgg agcgcctccc cacggtcgac cgggtgctcg tgttcgtcgc
     1561 cgccctcggg ttcgtgccgg tcacctacga gatcatcacg ggccaggtga cgatgctgat
     1621 cgcggccgcc ctgtacccgg tccgcgagcg cgacggcttc gcccgcggga tcggcgtggg
     1681 cctcgtgctc gcgctgttcg ccaagccgat gctgctcccc gtgctggtct ggatgctgct
     1741 ctggcggcgc caggcgctgg tgggcacgat cggcgccgcg gcggccgtga ccgtgctggg
     1801 cgtcgtcctg ctcggtacgg gcatctatgg ggcgtggatc gatgccgtcg tcggcacggg
     1861 gaagatcgtg cggcccggca acatggcgct gaccgacctg gccccgcccg cggtcgtcct
     1921 ggggctgtcg gtgatcgcga tcgcgctcgc cggctgggcg atcatccgcg acgagcgacg
     1981 agggttcgtg gccgcgctga ccgccgcgat gctcgttgcg ccgttcacgc tcatgtacca
     2041 ggtctcgatc ctgctgctcg ccgtccgccc ggccctcgcc gtggtgcctc gcgccacgcg
     2101 catcctcgcc ctcgtcgcga acccggcggt gctcgcggtg ttcatcccgt gggccgctgc
     2161 ggtgctggtc gccgtcacgc cgttctcgcg gcggcgcccg gaatgacggc agccgcgacc
     2221 gctcccgcgc cacggctgtc gctcgccgcg gcgctcgccc tgatcggcgt cgccctcggt
     2281 gcgacgctgc tggtcctcgg tctcggctcg tggccgggca cgtactactc gcccatccgg
     2341 gtcgcatcga tcgtggtggc gttcgccggc ctcgtcatct ggctggtcat cgcgctcgtg
     2401 cgccccgagc atcggcccgc cagcggcctc gcgggcgcgt tcgcggcggc cctgttcgcg
     2461 ttcgcgatca gcgcgatcaa cgctcagaac gtccgtctcg cgttcgactt cctgggctat
     2521 gccgtgctcc tgacggggct gtacctgctg ttcgtccggg tgtggacatc gcacgcgatc
     2581 gccctgcgcc tcgaagcgct gctcgcgctc gcctgcctgt tgggtggcgt gctgttcatc
     2641 gccgcgatgg tgaacctgtg ggccacgatg tggtccgcga tcggggcgat cgtgatgcca
     2701 ccgctgcggt tcggctccga ggggctgtgg accgggacgc cgaacgccat ggccgccttc
     2761 caggtgctcc tctactgcgg cgtcgtcacg tccctgctgg cgcgcgggcg ccgtgggcgg
     2821 atcgtggccg tcgcgctcgg catcgtcgtc gtcgtcgatg tcgccatcag cggatcgagg
     2881 ggggcatggc tcggcgtcgc gctcgcggtt gtcgtgaccg ggaccgtgtg gttggcgacg
     2941 accgaccgcg accgcctgcg cggcacgtgg agccggctgt ggagcggtcg ctcgcgagtg
     3001 ttcgtgatcg tgggcgcggt cgtcgcggcg ctcgcggcgt cggccgcggt cgtcgttgcg
     3061 ctcccgcggc tcagcgaggg cgggggcgac ctccgctcag atttcatcag cgtatcgatc
     3121 cgcatgttcc aggcggctcc cctgacgggc cagggcccgg gcagctgggg tcccgagcgg
     3181 ctcgcctaca cggacgcgtc ccagatcgac tactacgtcc cgcacgcgca caacgtgcct
     3241 gcccagacgg tggccgagtt cggcctcgtt gggatcgtcg ccgggatcat cgtggtcgcg
     3301 ctcgtcctgc ggctggtcgc gcacggcctg cggggccgct cccccgctga gcgggggctc
     3361 gcgctcgccg cgctgttcgc gtgcgtgtac atcaccggcc agcagctcgt ggacgcctgg
     3421 atccaccagc ccgcgatcct gttcgcgctg gccgtgccca tcgcgcgtct cgacgcggcg
     3481 ctgctccggg cagagaccgc gccgtacccg tggacgaagc ggctcctgtc ggtcgtgctc
     3541 ctcgtggcgg tcgtggtggg aacagggacc gccctctggc ccgagccagc ggcgcagctc
     3601 ggcctggagg ccgtcggggc atccgagaag ggcgattggc agcgggcgct cgacgagtcg
     3661 accgcggcgg tcaccgccga cccgcgcatg ccgccatatc agctcatgcg cggcctcagc
     3721 gcggcgcatc tcggcgacat gcagacggcg cgcgaggcgt tcgcggcggc gacggccgac
     3781 gacctcccgg ccagttggat caacctggcg gcggtccagc tgcggcttgg cgatccggtt
     3841 ggggcggcgg cgtcgctgga tcgcgggacc cggctcggga tccagcagcc cgtggtcgcc
     3901 acggccgccg cgggcctgta ccacgagctt ggcgacgacg acgaagccgc gcgcatgctg
     3961 gcggcggcgt ttggcgccgt cccgagcctc agtgccgacg cgatgtggcg agaccccgcg
     4021 tgggcgccgg tcgcgcagca ggccgtcgat gccgcggtcg caggcgacga ccggaccgcc
     4081 gcgatgcgcg tcgcgctcga gtccggccgc ttcggcgccg cgcgcgccat cgcgcacgag
     4141 atcgacccgg acgacagcga cctcgcccag acgacggtcg ccgcgtgggc gggtgaccag
     4201 ggggcgttca ggaagctcca cgaggccgcg cgcgccgatc ccgtgaatgc gccgctcgtg
     4261 gagatgtgtc gccgggtcgc ccgtgtccac gacccgagcc tctcaacccc tggctgggcg
     4321 tgcgacggcg gctggtactt cggcgcgtac cccgtggcga cgatcggcag cggtccactc
     4381 gaggcgtccc tgcccggcgc caacgagtac tggcagggcc tctacgtcta tcgccggccc
     4441 ggcccggccg acctactcgt gccgtgggtg ctccacatcc agtcgacgca cgcctgaggg
     4501 ccggcctcag cgtcgacgca tccgtcggcg cgcgacctcc tcgcgagcga tcccgatgac
     4561 gaacttcgag ttggtgacca cgtagcgccc gagcaggcgt cgggggtcca tcgccagccg
     4621 gaacagccac tcga
//
LOCUS       DBNHXO010000029         2638 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_998617, whole genome shotgun sequence.
ACCESSION   DBNHXO010000029 DBNHXO010000000
VERSION     DBNHXO010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_998617"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="AC162A_00635"
     CDS             <1..265
                     /locus_tag="AC162A_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009373.1"
                     /translation="IDVSSVLDQRWAAITEHVTQISTENPFVRFGRDAWAEFWSREAY
                     IRRESRVPAPDAETDLFAGLESATPGPLGWGRTADELAAIGAP"
     gene            complement(656..2419)
                     /locus_tag="AC162A_00640"
     CDS             complement(656..2419)
                     /locus_tag="AC162A_00640"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR5009374.1"
                     /translation="MSDDEPRYDARQKPAPSRYGAHPIRPEPRRPAWLIPAALIGGLA
                     LVAVVIGVGLFGGGSGGPNGTPSAVPSGIAAVPSAASPADSPAPSDGDTSASQSPGES
                     AGPSASPDAPAELVDAAVAVAPVTSFRSPLTSVKPIDVRALVGGGNAFDHVVLVEADA
                     DAILDAVRLDREALGKKLKIVASPEALAKYLASHRSDIGFLRADDVGPSVKALGWGQA
                     SLFGGDRVKSLDAWPLQATLKIPDGQPPAYDPARAWTMVAGGDVLLDRGVKLAIDDHA
                     AGTNYPFNGGTVDITGTCKDCSPLGWDTPYTQRTGNAGAFRELTKGADIAIANFENPA
                     PNDWRFHPSGTVFSANPANIAALSDAGFDWVSMANNHIGDAGDDGILQTMKNLNKFDI
                     AHAGAGRNTKAAHKAALIEAGGVTVGLLGYDTIAPSYWSASDSPGSAQMTVGALKRDI
                     ARARAAGAEVVVVMPHWGVEYTARPTAQQQKLGRAAINAGADMVVGNHPHWVGAMEVY
                     KGKPIWYAMGNLVFDQSWSIPTSEGITVENTFNGTQLVQSRLRPHLILDKAQPNLLDP
                     AGDGREVLRQLYKASGDLLPW"
     gene            complement(2503..2579)
                     /locus_tag="AC162A_00645"
     tRNA            complement(2503..2579)
                     /locus_tag="AC162A_00645"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2543..2545),aa:Asp,seq:gtc)
BASE COUNT          369 a          917 c          960 g          392 t
ORIGIN      
        1 gatcgacgtg tcgtcggtcc tcgaccagcg ctgggcggcg atcacggagc atgtgaccca
       61 gatctcgacc gagaacccgt tcgtgcgctt cggccgtgac gcatgggccg agttctggag
      121 ccgcgaggcg tacatccggc gcgagtcccg ggtcccggcg cctgacgcgg agaccgacct
      181 gttcgcgggc ctcgagtcag cgaccccagg cccgctcggc tggggccgca ccgccgacga
      241 gctggccgca atcggcgccc cgtagcgccc ggccaccgct gaccgtcgtg acccggcggg
      301 cgcggcgagc tggccaagta ttcgccgggt gaatgcgcct caacggttcg gccacaaggc
      361 cgtctgcgcc cgcgggcacg cgtgcgtggc ggccacgtcg gatcctgcct tgtattcctg
      421 acgtgagtac taggcgaagt acggtcccgc gcacttggcc caccattcgt ccggcgcatg
      481 caaggcacac caggggcccg gatcctgccg ctctcagtca ccgggtgaat gcatggcagg
      541 tcggggcgca cccgcattca accggtgaat gcatgtccga gtcggcggtg gcaggggccc
      601 aggcggcggt ggcgaggtgc cggccggcgc ccgacgaggt cgcgccggtc ggctgctacc
      661 agggcaggag gtcgcccgac gccttgtaga gctgcctcag cacctccctg ccgtcgcccg
      721 cagggtcgag gaggttcggc tgggccttgt cgaggatcag gtgcggtcgg aggcgcgact
      781 ggacgagctg cgtcccgttg aacgtgttct cgaccgtgat cccctccgag gtcgggatcg
      841 accacgactg gtcgaacacg aggttgccca tcgcatacca gatcggcttg cccttgtaga
      901 cctccatcgc accgacccag tgcgggtggt tgccgaccac catgtccgcg ccggcgttga
      961 tcgccgcccg gccgagcttc tgctgctgcg ccgtcggccg cgccgtgtac tcgacgcccc
     1021 agtggggcat cacgacgacg acctctgctc cggccgcacg ggcccgggcg atgtcccgct
     1081 tcagggcgcc gaccgtcatc tgcgcgctgc cggggctgtc ggacgccgac caatacgagg
     1141 gcgcgatcgt gtcgtagccg agcaggccga cggtcacgcc acccgcctcg atcagcgccg
     1201 ccttgtgcgc ggccttggtg ttccgccccg cacccgcgtg cgcgatgtcg aacttgttga
     1261 ggttcttcat cgtctgcagg atcccgtcgt cgccggcgtc gccgatgtgg ttgttggcca
     1321 tcgacaccca atcgaagccg gcgtcgctga gcgcggcgat gttcgcgggg ttggccgaga
     1381 acaccgtccc cgacgggtgg aagcgccaat cgttgggcgc cgggttctcg aagttggcga
     1441 tcgcgatgtc cgcgcccttg gtgagctcgc ggaaggcccc ggcgtttccg gtgcgctgcg
     1501 tgtacggggt gtcccagccc agcggcgagc agtccttgca ggtgcccgtg atgtcgaccg
     1561 tgccgccgtt gaacgggtag ttcgtgccgg ccgcgtggtc gtcgatggcg agcttcacgc
     1621 cgcgatcgag caggacgtcg ccgcccgcga ccatcgtcca tgcgcgcgcc gggtcatagg
     1681 ccggcggctg gccgtcggga atcttgaggg tcgcctggag cggccacgcg tcgagcgact
     1741 tcacccgatc gccaccgaac agcgaggcct ggccccagcc cagcgccttg accgatggcc
     1801 cgacgtcatc ggcacgcagg aagccgatgt cggaacggtg acttgcgagg tacttcgcga
     1861 gggcctcggg cgaggcgacg atcttgagct tcttgccgag cgcctcgcga tccagcctga
     1921 cggcgtcgag gatggcgtcg gcgtcggcct cgaccaggac gacgtggtcg aacgcgttcc
     1981 cgccacccac gagcgcccgg acgtcgatgg gctttaccga cgtcagcggc gaccggaagc
     2041 tggtcaccgg cgcgacggcc acggccgcgt ccacgagctc ggccggtgcg tccggcgacg
     2101 ccgacgggcc tgcggactcc ccgggcgact ggctggcgct ggtgtcgccg tccgacggag
     2161 cgggcgagtc cgcgggcgac gccgccgacg gcaccgcggc gatgccgctc gggacggccg
     2221 acggcgttcc gttgggccct cccgacccac cgccgaacag gccgacgccg atcaccacgg
     2281 caacgagggc gaggcccccg atcagggcgg ccgggatcag ccacgcaggg cgtcggggct
     2341 cgggacggat gggatgggcc ccatacctcg acggcgcggg cttctgacgc gcgtcgtagc
     2401 ggggctcgtc gtcgctcaca ggcggcagcg tacccgaccg atcggaaaag gcggcgcccc
     2461 gtccgggcgg gcggacgggg cgcgagagga agcgaggaag gatggcggac ccgaccggat
     2521 tcgaaccggc gatctccagc gtgacaggct ggcatgttgg gccgctacac cacgggtccg
     2581 cagccggtgg cggatgatag cagggacccc atggcgccgc cagcgatcct gaggggtt
//
LOCUS       DBNHXO010000030         3501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1990753, whole genome shotgun sequence.
ACCESSION   DBNHXO010000030 DBNHXO010000000
VERSION     DBNHXO010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1990753"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1093)
                     /gene="ilvC"
                     /locus_tag="AC162A_00650"
     CDS             complement(47..1093)
                     /gene="ilvC"
                     /locus_tag="AC162A_00650"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR5009375.1"
                     /translation="MYYDADADPRALDGQTVAIIGYGSQGHAHALNLRDSGVDVVVGL
                     APGSKSRALAEAAGLRVEDVADAVKAADVIMIAVPDTAQKAVYDAEIEPNLRPGQLLM
                     FAHGFNIRFARIQPGATIDVGMVAPKGPGHLLRSVYEQGGGVPALFAVEQDPSGTARA
                     RVLAYARGIGSTRAGVLETTFKEETETDLFGEQALLCGGVSALVKAAFETLVEAGYQP
                     ELAYFETMHELKLIGDLMYRGGLNFMRFSVSDTAEYGDYVSGPRVTEGAKAAMKDVLS
                     DIQSGSFATRWIAVQDAGGEEFRRLREQDRHHQIEQVGADLRAKMPFLKPVVVEAGQA
                     QAVASAVPAGEGSR"
     gene            complement(1102..1698)
                     /gene="ilvN"
                     /locus_tag="AC162A_00655"
     CDS             complement(1102..1698)
                     /gene="ilvN"
                     /locus_tag="AC162A_00655"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR5009376.1"
                     /translation="MSADDGSNGQHPQPAHTSPPPPRALPGTGEAARHILVVLVMNRP
                     GVLNRVASLMRARNFNIDSLAVSRTDRESVSRMTITIHGDAVAVEQATKQLYRLVDVL
                     KVQDVTADQLVEHELALVKVRAGDANRAEVIKIVELYKGRVVDIAPDSVIVEATGTEG
                     EIDALVALLRGYGIKELVRTGAVVMLRGAGSIEEALKL"
     gene            complement(1695..3443)
                     /gene="ilvB"
                     /locus_tag="AC162A_00660"
     CDS             complement(1695..3443)
                     /gene="ilvB"
                     /locus_tag="AC162A_00660"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018725895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HIR5009377.1"
                     /translation="MLRDVRPRKRTRIGADALMEALIRQGTDVLFSYPGGVILPIYDI
                     LGDYPEIRHILVRHEQGGGHAADGYARASGRVGVCMGTSGPGATNLVTAIATAQLDSI
                     PMVAITGNVPGALIGKDAFQEIDITGITLPMTKHNYLVRNADDIPHVIAEAFHLARSG
                     RPGPVHVDITKDALQQETRAEHPSLDDVIAGLPGFRPTLDGHPKQLKLAAREIAAAKR
                     PLILAGHGVLIADGGEELRAFAEKTSIPVAWTLLGIGALDERHPLAYGYMGMHGWKHV
                     NRAIQSADLLIAVGMRFDDRVTGNVRTYAPYARIVHADIDPAEIGKNVAVEVPIVGDA
                     RRVLAGLSALVPATPLEERRAYLDELAEWKQESLGSSWHGSGAWRDGLLSADFVVERI
                     GELTDHTANYVADVGQNQMWLARYAGFHRPNSHLSSGGLGTMGYCVPAAMGAALGRPE
                     FETWAVTGDGGFQMTSQELMTLAADRIPVKIAILDNKKLGMIRQWQEIIYAGNYHSAH
                     LPGPDFIKLAEAYGIPAFKATTTADVDTTIKAAQAVDGPALIWFEIAEEQNVFPMMPA
                     GKGLSDLIETWGAPEE"
BASE COUNT          459 a         1231 c         1250 g          561 t
ORIGIN      
        1 ccggcggcga cgccgccacc gaacctcggt tgcgtggcct cgaaggtcat cgcgacccct
       61 cccccgccgg taccgcgctg gccaccgcct gcgcctgtcc ggcctcgacg acgaccggct
      121 tgaggaacgg catcttcgct cgcaggtcgg ccccgacctg ctcgatctgg tggtgccgat
      181 cctgctcgcg cagccgtcgg aactcctcgc caccggcgtc ctggaccgcg atccagcggg
      241 tcgcgaacga cccgctctgg atgtcggaca gcacgtcctt catcgcggcc ttggcgccct
      301 cggtcacccg cgggcccgag acgtagtcac cgtactcggc ggtatcggag acgctgaagc
      361 gcatgaagtt gaggccgccg cggtacatga ggtcgccgat gagcttgagc tcgtgcatcg
      421 tctcgaagta cgcgagctcg ggctggtagc ccgcctcgac gagcgtctcg aaggcggcct
      481 tgacgagggc agacacgccg ccgcacagca gcgcctgctc gccgaacagg tcggtctcgg
      541 tctcctcctt gaacgtcgtc tcgagcaccc cggcgcgggt cgagccgatg ccccgcgcat
      601 aggcgagcac gcgggcacgc gccgtccccg acgggtcctg ctcgaccgcg aacagcgccg
      661 gcacgccgcc gccctgctcg tagacgctgc gcaggaggtg gcccgggccc ttgggcgcga
      721 ccatgcccac gtcgatcgtt gcgcccggct ggatccgcgc gaaccggatg ttgaacccgt
      781 gggcgaacat cagcagctgg cccggccgga ggttgggctc gatctcggcg tcgtagaccg
      841 ccttctgggc cgtatccggc acggcgatca tgatcacgtc ggcggccttg acggcgtcgg
      901 cgacgtcctc gacccgcagc ccggccgcct cggcgagggc gcggctcttg gagcccggcg
      961 cgagcccgac gacgacgtcg accccgctgt cgcgcaggtt gagggcgtga gcgtggccct
     1021 ggctgccgta gccgatgatc gcgaccgtct gcccgtcgag ggcgcgcgga tccgcgtcgg
     1081 cgtcgtagta catccgcgct gtcacagctt gagtgcctcc tcgatcgagc ccgccccgcg
     1141 cagcatgacc accgcgccgg tccggaccag ctccttgatc ccgtagccgc gcagcagcgc
     1201 gacgagggcg tcgatctccc cctccgtccc cgtcgcctcg acgatcacgg agtccggcgc
     1261 gatgtccacg acgcgcccct tgtacagctc gacgatcttg atcacctcgg cccggttggc
     1321 gtcgccggcg cggaccttga ccagtgcgag ctcgtgctcg accaactggt cggcggtgac
     1381 gtcctggacc ttgagcacgt ccaccagccg gtagagctgc ttggtcgcct gctccacagc
     1441 gacggcgtcg ccgtggatcg tgatcgtcat ccggctgacg ctctcgcggt cggtccggga
     1501 cacggcgagc gagtcgatgt tgaagttgcg tgcccgcatg aggctcgcaa cccggttgag
     1561 gacgccgggc cggttcatca ccaggacgac gaggatgtgg cgggctgcct cgcccgtgcc
     1621 ggggagcgcc cgcggcgggg gcgggctcgt gtgcgccggc tgggggtgct ggccgttgga
     1681 tccgtcgtcg gcgctcattc ctcgggcgct ccccaggtct cgatcaggtc ggacagcccc
     1741 ttgccggccg gcatcatcgg gaacacgttc tgctcctcgg cgatctcgaa ccagatcagc
     1801 gccggcccgt cgaccgcctg ggcggccttg atggtcgtgt cgacgtcggc ggtcgtggtc
     1861 gccttgaagg ccgggatccc gtacgcctcg gcgagcttga tgaagtccgg cccgggcagg
     1921 tgggcggagt ggtagttgcc cgcgtagatg atctcctgcc actggcggat catcccgagc
     1981 ttcttgttgt cgaggatcgc gatcttgacc gggatccggt cggccgccag cgtcatcagc
     2041 tcctggctgg tcatctggaa gccaccatca ccggtcacgg cccacgtctc gaactccggc
     2101 cgcccgagcg ccgcgcccat cgccgccggg acgcagtagc ccatggtgcc gaggccgccc
     2161 gagctcaggt ggctgttcgg ccggtggaag cccgcgtagc gcgcgagcca catctggttc
     2221 tgaccgacgt cggccacgta gttcgcggtg tggtcggtca gctcgccgat ccgctcgacg
     2281 acgaagtcgg cggacagcag gccgtcgcgc cacgcgccgg agccgtgcca cgagctgccc
     2341 agcgactcct gcttccactc ggcgagctcg tcgaggtagg cgcgccgctc ctcgagcggc
     2401 gtcgctggaa cgagcgccga gaggccggcg aggacccgcc gcgcgtcgcc gacgatcggc
     2461 acctcgaccg cgacgttctt gccgatctcg gccgggtcga tgtccgcgtg gacgatccgc
     2521 gcgtagggcg cgtaggtccg gacgttgccg gtcacccggt cgtcgaagcg catgcccacg
     2581 gcgatcagga ggtcggccga ctggatggcc cggttcacgt gcttccagcc gtgcatgccc
     2641 atgtagccgt aggccagcgg atggcgctcg tcgagggcgc cgatgccgag cagggtccag
     2701 gccacgggga tgctggtctt ctcggcgaac gcccgaagct cctcgccgcc gtccgcgatc
     2761 agcacgccgt ggccggcgag gatcagcggg cgcttggcag cggcgatctc gcgggcggcc
     2821 agcttgagct gcttggggtg gccgtcgagg gttgggcgga agcccggtag gccggcgatg
     2881 acgtcgtcga gggagggatg ctcggcgcgc gtctcctgct gcagggcgtc cttggtgatg
     2941 tccacatgga cgggacccgg acggccggag cgggcgaggt ggaacgcctc ggcgatgacg
     3001 tgcgggatgt catcggcgtt ccggacgagg tagttgtgct tggtcatcgg cagcgtgatg
     3061 ccggtgatgt cgatctcctg gaacgcgtcc ttgccgatga gcgcgcccgg gacgttgccg
     3121 gtgatcgcaa ccatcgggat cgagtccagc tgggcggtgg cgatggccgt gaccaggttc
     3181 gtcgcgcccg gaccgctggt gcccatgcac acgccgaccc ggccgctggc ccgcgcgtac
     3241 ccgtcggcgg cgtgcccgcc gccctgctcg tggcggacga ggatgtgccg gatctcaggg
     3301 tagtcgccga ggatgtcgta gatcggcagg atgacgccgc cgggatagct gaacaggacg
     3361 tcggtcccct ggcggatgag cgcctccatc agcgcgtcgg cgccgatccg cgtgcgcttg
     3421 cggggacgca cgtcccggag catgtccttg cgcgcctgct cgaccgccgc cgcgcccgcc
     3481 gatccgtcgg ccggcacgtg g
//
LOCUS       DBNHXO010000031         4042 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_404425, whole genome shotgun sequence.
ACCESSION   DBNHXO010000031 DBNHXO010000000
VERSION     DBNHXO010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_404425"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="AC162A_00665"
     CDS             complement(<1..652)
                     /locus_tag="AC162A_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019547303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR5009378.1"
                     /translation="MPVPVVEFTDERLPNGLRLIVAEDHLAPVVAVNVWYNVGSKNEV
                     PGKTGFAHLFEHVMFQGSAHVGKAEHMALIQAAGGTLNGTTWLDRTNYYETMPSHQLE
                     LALFLEADRMGSLLHALSQENLDNQREVVKNEKRWSYDNRPYGTWNEKLLGHLYPEGH
                     PYHHPTIGSMADLDAASVDDVSAFFRTWYAPNNAVLSVVGDCDRAQVRAWADKYFGP"
     gene            1129..2154
                     /locus_tag="AC162A_00670"
     CDS             1129..2154
                     /locus_tag="AC162A_00670"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5009379.1"
                     /translation="MVRDVGAGTGMPARFDVRTAFDFAISLASDVGQHDELPDEDRRW
                     LEKARRALPDHLRPATDSELCIFGTGLIVDLPEVADAAAFAALLKRTPGREFAAAVLA
                     DQLRDPAREAEIDAALDGDRTAVDAVLASWPEHKHGWLSRILGDGDELVGEVSDLLEA
                     WLPLYQEIEPRVASIIERDVALRAGDRARLRPADLIERTTNGIRWLSEPGVRRVVLAP
                     SYLARPYNFTFAGGDWRMFVYPVSDGALEPSDPLAPPAGVLRLHRALGDDTRLRILRL
                     LRDRDWYLTEIAERLELSKPTIKHHLAQLRAAGLVTLTEEGGLSYYSLRRDRLDDAST
                     ELKHYLT"
     gene            2261..2464
                     /locus_tag="AC162A_00675"
     CDS             2261..2464
                     /locus_tag="AC162A_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009380.1"
                     /translation="MYQTTNPTEVNPLVGDRIHDLYETSSELRASRGPRNTSDRAGLL
                     DRTRWTIGRRLISIGSAVAGQHA"
     gene            2625..3278
                     /locus_tag="AC162A_00680"
     CDS             2625..3278
                     /locus_tag="AC162A_00680"
                     /inference="COORDINATES: protein motif:HMM:NF016149.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0046688 - response to copper ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HIR5009381.1"
                     /translation="MSTKPSSIVRPSLIGALLAIVALGLAAGTALAHAELDTADPADG
                     AVLDTPPTTIRLTFTERLDASKSSFRLLLGDDNLGVGEVAGPRAMTLDGLTLGPGKYL
                     IKWTSASADDGDIERGQLTFTVEEPPASVEPSASPAPTPEPSASAAPSTAPSAEPTTA
                     PTPAASVATSPAAPTPAPSAAPTEPASSTSDVLLPIVVGLLLVGGVGAFVLRRSRGA"
     gene            3289..>4042
                     /locus_tag="AC162A_00685"
     CDS             3289..>4042
                     /locus_tag="AC162A_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009382.1"
                     /translation="MGLIAGAVRPALARAAGIVAALAVSLGAPAVVAAHNLGTVYQSP
                     LPLAVYLAGAATTVALSFVFVLARDMRAAPTPPGRIVHVPAPLRIALRAAGLLGWFWI
                     VAQGIAGGTSEASVATLFLWVYGWVGVAMLSALLAPVWEWVDPFATLHDILAWGLRRL
                     GIRGWPASELPPAIRLWPAVLGLMFFIWLELVAVAGNSTLTVVLVGYTLLTLALMAQF
                     GRDQWRAQGETFTVWFRTLNRLAIYGVVPAAQT"
BASE COUNT          554 a         1503 c         1374 g          611 t
ORIGIN      
        1 tcgggccgaa gtacttgtcg gcccacgcgc gcacctgggc gcgatcgcag tccccgacca
       61 ccgacagcac cgcgttgttc ggcgcgtacc aggtccggaa gaacgcgctg acgtcgtcga
      121 ccgaggcggc gtcgaggtcg gccatcgacc cgatcgtcgg gtggtgatac ggatgcccct
      181 cggggtacag gtgcccgagc agcttctcgt tccaggtgcc gtacggccgg ttgtcgtaac
      241 tccagcgctt ctcgttcttg acgacctcgc gctggttgtc gaggttctcc tggctgagcg
      301 cgtggagcag cgagcccatc cggtccgcct cgaggaacag ggccagctcg agctggtgcg
      361 agggcatcgt ctcgtagtag ttggtgcggt cgagccaggt cgtcccgttg agcgtgccgc
      421 cggccgcctg gatgagcgcc atgtgctcgg ccttgccgac gtgcgcggac ccctggaaca
      481 tcacgtgctc gaacaggtgc gcgaacccgg tcttgcccgg aacctcgttc ttggagccga
      541 cgttgtacca gacgttgacg gccaccacag gcgcgaggtg gtcctcggcg acgatcaggc
      601 gcaggccgtt gggcaggcgc tcgtcggtga actcgacgac gggaacgggc atgcgaagcg
      661 ggacctcggg cgacgcgggg ctgggaggcc gattgtatgg cgaccccgtc gtcggggcgc
      721 gtttcaccga cgccgtggtg actcgcaggc aacgctctag ggtcgaacgg cgtcccgggc
      781 ggagcctcgc ggcggtttcg tgcctcctgg gcacgcgcga gtcccgtagg aagtcgcggc
      841 gcccttgggg acccggattc gttgcctggc atgcacgatc agcggtcggg ctggctggcg
      901 agcggccatg tgccgcactt cgttgcctgc caagcacgac gtcgggacgg ccctgagaac
      961 gaaccacgcg cgacagtcgc gcagggcttg acaggtgcgg agtcgatgtt agatagtcgt
     1021 ctaacacgag ttcgatcagt tcgaacgacc actccaaccg ggacaccgat gacaccctct
     1081 ccccgccaga tcacgccgag caccgcgacc gccccgagcg ggcgtgcgat ggtgcgcgac
     1141 gtcggggcag gcaccggtat gccggcccgg ttcgacgtgc ggaccgcgtt cgacttcgcg
     1201 atcagcctcg cctccgacgt cggccagcac gacgagctgc ccgacgagga ccgccgctgg
     1261 ctcgagaagg cccgcagggc cctccccgac cacctccggc cggccactga ctccgagctg
     1321 tgcatcttcg ggacgggcct gatcgtggat ctgcccgagg tcgcggacgc cgccgccttt
     1381 gccgcgctgc tcaagcggac gccgggtcgc gaattcgccg cggccgtgct cgcggaccag
     1441 ctgcgcgacc cggcccgaga ggcggagatc gatgctgccc tggatggcga ccgaaccgcc
     1501 gtcgatgccg tccttgcctc ctggcccgag cacaagcacg gctggctcag ccggatcctc
     1561 ggcgacgggg acgagctcgt gggcgaggtc agtgacctcc tggaggcctg gctcccgctg
     1621 taccaggaga tcgagccgcg cgtcgccagc atcatcgagc gggacgtcgc cctccgggcg
     1681 ggcgatcgcg cccgccttcg ccccgcggac ctgatcgagc gcacgaccaa cggcatccgc
     1741 tggctgtcgg agccgggcgt gcggcgcgtc gtgctcgcgc cgtcgtacct ggcgaggccg
     1801 tacaacttca ccttcgccgg cggcgactgg cggatgttcg tctacccggt ctcggacggg
     1861 gccctcgagc ccagcgaccc gctggcgccg ccggcaggcg tgctgcgcct gcaccgcgcg
     1921 ctgggcgacg acacgcggct ccggatcctg cggctcctgc gggaccgtga ctggtacctg
     1981 accgagatcg ccgagcgcct cgagctcagc aagccgacca tcaagcacca cctcgcgcag
     2041 ctccgggccg cgggcctcgt gacgctgacc gaggagggcg gcctctccta ctacagcctc
     2101 cgacgcgacc gtctcgacga cgcgtcgacc gagctcaagc actacctcac ctaagtcccc
     2161 ccacattcat gccgcgtttc gacgcggcgc cgagttagat gagtacctaa cacacgtaag
     2221 accagcaacg gactggaagg acaccgcgag gacgacgccc atgtaccaga cgacgaaccc
     2281 gaccgaggtc aatcccctcg tcggggaccg catccacgac ctgtacgaga cctcgagcga
     2341 gctgcgcgcc agccggggac ccaggaacac gtccgaccgg gcggggttgc tggaccggac
     2401 ccgctggacg atcggacgtc gcctgatctc gatcggcagc gccgtcgccg gccagcacgc
     2461 ctgatccacc gggccgacgc ccggccagtc agcggggcgg ccggtcagcc gcgaccccgg
     2521 tcgccttgct cctcgaggcg ggccggcgtt gcgggtgacc ggccgtttcg cgtccggcgc
     2581 ccgggaaccg cacggaatcg gagccgcggc tagactccgc tctcgtgagc acgaagccca
     2641 gttccatcgt ccgtccatcc ctcatcggcg cgctcctggc gatcgtcgcc ctcggcctcg
     2701 ccgccggcac tgcgctcgcc cacgcggagc tcgacaccgc cgatccggcc gacggcgccg
     2761 tcctcgacac cccgccgacg acgatcaggc tcacgttcac cgagcggctc gacgcatcga
     2821 agagctcgtt ccggctcctg ctcggtgacg acaaccttgg cgtcggtgag gtagcaggcc
     2881 cgcgcgcgat gacgctggac ggtctcacgc tcggccccgg caagtacctg atcaagtgga
     2941 cttcggcgtc cgccgatgac ggcgacatcg agcgcggcca gctgaccttc acggtcgagg
     3001 agccgccggc ctccgtcgaa ccctcggcga gccccgcgcc caccccggag ccgtccgcat
     3061 cggccgcccc atccactgcg ccctccgccg aaccaacgac cgccccgacg cccgctgcct
     3121 cggtcgccac atcgcccgcc gcgccaaccc cggccccgtc ggccgcgccg accgagcccg
     3181 cctcgtcgac gagcgacgtc ctgctgccga tcgtcgtcgg gttgctgctc gtcggcgggg
     3241 tcggagcatt cgtgctgcgc cgaagccgcg gggcctagct cgatcggcgt gggcctgatc
     3301 gcgggagccg tgcgcccggc cctggcgcgc gccgcgggga tcgtcgccgc cctcgcggtc
     3361 tccctcggcg ccccggcggt cgtggcggcc cacaacctcg gcaccgtcta ccagagtccg
     3421 ctgcccctcg ccgtctacct cgcgggggcg gcgaccaccg tcgcgctgtc gttcgtgttc
     3481 gtgctcgccc gtgacatgcg cgcggccccg accccgcccg gccgcatcgt ccacgtgcct
     3541 gcgccgctgc ggatcgcgct ccgggccgcc ggtctacttg gatggttctg gatcgtggcc
     3601 caggggatcg ccggcgggac ctcggaggca tcggtcgcga cgctgttcct gtgggtctac
     3661 ggctgggtcg gcgtcgcgat gctgtcggcg ctgctggcgc cggtctggga atgggtcgac
     3721 ccgttcgcga cgctgcacga catcctcgcc tggggccttc ggcgccttgg catccgcggc
     3781 tggcccgcct cggaactccc gcccgcgatc cgcctctggc cggccgtcct cggcctcatg
     3841 ttcttcatct ggctcgagct cgtcgccgtc gccggcaaca gcaccctgac ggtggtgctc
     3901 gtcgggtaca cgctgctgac actggccctg atggcgcagt tcggacgcga ccagtggcgc
     3961 gcccagggtg agacgttcac ggtctggttc cgcacgctca accgactcgc gatctacggc
     4021 gtcgtgccgg cggcgcagac cg
//
LOCUS       DBNHXO010000032         3529 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2586302, whole genome shotgun sequence.
ACCESSION   DBNHXO010000032 DBNHXO010000000
VERSION     DBNHXO010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2586302"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="AC162A_00690"
     CDS             <1..1061
                     /locus_tag="AC162A_00690"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR5009383.1"
                     /translation="HGAVGMLADRERRHAAELEAAIGALGEGILVTDPDGSIRMLNPA
                     AIRLLAGPVSSIEGLLDRLLDADDERPAALDRGPAEYRLIGRPPAWIEVTSYPVAEAA
                     GLASPSTVIVCRDVTAFRQGQALREAFLGLLSHELRTPVTTIYGGAAVLSKPGKTLDP
                     AMANEILADIASESDRLYRLVEDLLVLARFDEGFELGDEPALLQHLVPSVVDQERGRW
                     PLVTFEVVVAPDLPAVSGDETSITQVLRNLLSNAAKYSAGAGPVTAVVELDPDGVAVR
                     VRDEGPGIDPLEAEAIFDPFFRSPSTAKMAGGAGIGLYVSRRLVGAMNGRIWATARPG
                     GGSEFSFVLPRYRSDEED"
     gene            1220..1993
                     /locus_tag="AC162A_00695"
     CDS             1220..1993
                     /locus_tag="AC162A_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5009384.1"
                     /translation="MASQPLVLVADDEPRITKLVAISLSEDGFRVITASNGEEALRKA
                     EEYRPDVVLLDIVMPDIDGIEVMGQLREGRAVPVILLTAKGSTADKAKGLDLGADDYI
                     AKPFHPDELAARIRAVLRRSTGASSPAGIVRFDDIEIDLERRMVLRNGELVQLSRTEW
                     LLLQHLAANAGKVVLHTELLTKVWGPEYRDDLQYLRVWVSRVRRKLGALPGEPGRIKT
                     FQGIGYLLDVDDPSAEVAKEPDKPGDSAAAESSAEVAAP"
     gene            complement(2036..2527)
                     /locus_tag="AC162A_00700"
     CDS             complement(2036..2527)
                     /locus_tag="AC162A_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012730.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR5009385.1"
                     /translation="MKRRSPLRHAMDRFMADPASVRNAAYVMIVFTVGIVLVGGVIVW
                     VFDSRDFPDLGGALWYTLQTVTTVGYGDKVPTEGVGRLVGASVMVVAVALIAILTASI
                     TSTFVEAAQRRNRAGLEAQERDSAEALQQQLDEIVARLAAIETELKRPDARAATAVEE
                     HRP"
     regulatory      2638..2744
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2826..>3529
                     /locus_tag="AC162A_00705"
     CDS             2826..>3529
                     /locus_tag="AC162A_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR5009386.1"
                     /translation="MALELASSTAAPTADAAAAAPRIRGLICRNCGTPEVIGPNYVCA
                     ACFGPLEVDYDLAQVGRLVDPAAIAGRRPGIWRYLELLPVDAAPERGLAVGSTPLLRA
                     SRLGASIGIEGLLIKDDTRNPTLSFKDRAVAIAAARVASFGLEALGCASTGNLAGATA
                     AAAAAIGVPAFVFVPADLEPAKIEHALAYGATVVRIDGTYDDVNRLCLEVADELPWGL
                     VNVNLRPFYAEGSKTLG"
BASE COUNT          502 a         1298 c         1222 g          507 t
ORIGIN      
        1 tgcacggcgc cgtcgggatg ctggcggatc gcgaacggcg ccacgcggcg gagctcgagg
       61 cggcgatcgg cgccctgggc gagggcatcc tcgtgaccga ccccgacggc tcgatccgga
      121 tgctcaatcc agccgcgatc cggctcctcg ccggccccgt gtcatcgatc gaggggctgc
      181 tggaccgcct gctcgatgcc gacgacgagc ggccagccgc gctcgaccga ggacccgcgg
      241 agtaccgcct gatcgggcga ccgccggcgt ggatcgaggt cacctcgtac cccgtcgccg
      301 aggccgcggg cctcgcctcg ccgtccaccg tgatcgtgtg ccgcgacgtc accgcgttcc
      361 gccagggcca ggcgctccgc gaggcgttcc tgggcctgct gtcgcacgag ctgcggacgc
      421 ccgtgaccac gatctacggt ggcgcggccg tcctgtccaa gccgggcaag acgctcgatc
      481 cggccatggc gaacgagatc ctcgccgaca tcgcgagcga gtccgaccgc ctgtaccggc
      541 tggtcgagga cctgctcgtg ctggcgcgct tcgacgaggg gttcgagctg ggcgacgagc
      601 cggccctcct ccagcacctg gtgccctcgg tcgtcgacca ggagcgcggc cgctggccgc
      661 tggtgacctt cgaggtcgtc gtcgcgcccg acctccccgc ggtgagcggc gacgagacca
      721 gcatcaccca ggtcctgcgc aacctgctct cgaacgcggc gaagtacagc gcgggggcgg
      781 ggcccgtgac ggccgtcgtc gagctggacc ccgacggcgt cgcggtgcgg gtgcgggacg
      841 agggtccggg catcgacccg ctcgaggccg aggccatctt cgaccccttc ttccgctccc
      901 cgtcgacggc caagatggcc ggcggcgcgg gcatcggcct gtacgtgtcg cgccggctgg
      961 tcggcgcgat gaacgggcgg atctgggcga cggcgcgacc tggcggcggc agcgagttct
     1021 cgttcgtgct gccgcgctat cggagcgacg aggaggactg aacctccgcg gcgcgagggc
     1081 caatctgata tgccgatgta acgccgggtc gggacctgta ccgagccgcc cgtagcagcg
     1141 agcgggcatg atggctcgcg tgggcgtctg gccccggcgt gcttgcgcgt gggctccggg
     1201 cgtcgatcgg agtgtgtgca tggcgtccca gccgttggtc ctcgtggccg acgacgagcc
     1261 ccggatcacg aagctcgtcg cgatctccct gtccgaggac ggattccgcg tgatcaccgc
     1321 ctccaacggc gaggaggcac tgcgcaaggc cgaggagtac cggcccgacg tggtcctgct
     1381 cgacatcgtc atgccggaca tcgacgggat cgaggtcatg ggccagttgc gcgaggggcg
     1441 ggccgtgccc gtgatcctgc tgaccgcaaa gggatcgacc gccgacaagg cgaagggcct
     1501 cgacctcggc gccgacgact acatcgcgaa gccgttccat cccgatgagc tggccgcgcg
     1561 catccgcgcc gtgctccgtc gcagcaccgg ggcatcgtcg cccgccggga tcgtgcgctt
     1621 cgacgacatc gagatcgacc tcgagcggcg catggtgctg cgcaacggcg agctggtcca
     1681 gctctcgcgg accgagtggc tcctgctcca gcacctggcc gcgaacgccg gcaaggtcgt
     1741 cctccacacg gagctcctga ccaaggtctg ggggcccgag taccgcgacg acctgcagta
     1801 cctgcgcgtc tgggtcagcc gcgtccgacg caagctaggc gcgctccccg gcgagccggg
     1861 ccggatcaag acgttccagg ggatcggcta cctgctcgac gtcgacgacc cttcggccga
     1921 ggtcgccaag gagcccgaca agcctggcga ctcggccgcg gccgaatcct ctgccgaagt
     1981 cgccgccccc tgacggtccg gctcgacggt ggaccggcac ggccagctgg cgagcctagg
     2041 gacggtgctc ctccaccgcc gtggcggcgc gagcgtccgg tcgcttgagc tcggtctcga
     2101 tcgccgcgag cctggcgaca atctcgtcga gctgctgctg cagggcctcg gccgagtctc
     2161 gttcctgggc ctcaagtccg gcccgattgc gccgctgggc agcctcgacg aacgttgagg
     2221 tgatcgaagc cgtcaggatc gcgatcagcg cgacggcgac gaccatcacc gacgccccca
     2281 ccaatcgacc gacgccctcc gtcgggacct tgtcgccgta acccacggtt gtgaccgtct
     2341 gcagcgtgta ccagagtgcg ccgccgaggt ccgggaagtc ccgcgagtcg aacacccaca
     2401 cgatcacccc gccgacgagc acgatgccca cggtgaacac gatcatcacg taggcggcgt
     2461 tcctcaccga ggcggggtcg gccatgaacc ggtccatcgc gtggcgcagg gggcttcttc
     2521 gcttcatgcc gcgcagccta gcgcgccacg caaacccgcc tgcgggcgcc tggcgcagaa
     2581 ggagcggcgg ccttgacggt cgtccgcttc ccttcgtacc atccgcgcaa ctgaagagca
     2641 ctatcaagag tggcggaggg accggcccgt tgaagccacg acaacctacc ggcgggatgc
     2701 cggcaaggtg ccaattccgg gcaggtcttc ctgcgagata gggcgaaccc cctgggaccc
     2761 ttcctcgtgg agggtccttc tcgtttctcc ggtcccggcg tcgtctcccg aaaggaccgg
     2821 cttccatggc actcgagctc gcgagctcca ccgcagcgcc cacggccgat gccgcggcgg
     2881 cggcgccgcg catccgcggc ctgatctgtc gcaactgcgg cacgcccgag gtcatcgggc
     2941 cgaactacgt ctgcgccgcc tgcttcgggc ccctcgaggt ggactacgac ctggcgcagg
     3001 tcgggcgact cgtcgacccc gccgcgatcg cggggcgccg gccgggcatc tggcgctacc
     3061 tcgagctcct ccccgtggac gccgcgccgg aacgcgggct ggcggtcggc agcacgcccc
     3121 tgctgcgcgc gagccgcctc ggcgcgtcga tcggcatcga ggggctgctg atcaaggacg
     3181 acacccgcaa ccccacactg tcgttcaagg accgcgcggt cgccatcgcg gcggctcgcg
     3241 tcgcctcgtt cggcctcgag gcgctgggct gcgcgagcac cggcaacctc gcgggcgcca
     3301 ccgccgcggc cgcggcggcc atcggcgtgc ccgcgttcgt gttcgtcccc gccgacctcg
     3361 agcccgccaa gatcgagcac gccctcgcct acggcgcgac ggtcgtccgg atcgacggca
     3421 cctacgacga cgtcaatcgc ctgtgcctcg aggtcgccga cgagctgccc tggggcctcg
     3481 tcaacgtcaa cctccgcccg ttctacgccg agggcagcaa gaccctcgg
//
LOCUS       DBNHXO010000033         8884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4174531, whole genome shotgun sequence.
ACCESSION   DBNHXO010000033 DBNHXO010000000
VERSION     DBNHXO010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 8884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8884
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4174531"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..1756)
                     /locus_tag="AC162A_00710"
     CDS             complement(245..1756)
                     /locus_tag="AC162A_00710"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR5009387.1"
                     /translation="MAPDERPFPPGEYPAIVVGSGPGGLQTSYGLKHYGVPHAVISAD
                     PSPGGMFRRWPFFQRLLSWTKPYAPELATAREYQRYDWNSLIAFEPELRSLQAGFMDG
                     SSYFPSRPEMEASLAAFADRAGLAVRYDCRWERTRRDDGPDGPVFTLETSDGEYRCRT
                     LVLAVGIAQPWTPKQPGIEHARHYAETREASTYAGKRLLIIGKQNSGFELASGLAAWA
                     SKITVASPSPAKTSVQTKSLVGVRARYVQPFEDNFLGLGVSILDASIDSIEHVGDDYR
                     VMLKRTDNGESMSVDADEVIAATGFTCPLQDLTDLGVTTFGQAKLPAVTPMWESASVP
                     GIYFAGTVSSAAPGLKKHGIPAYSGAVQGHRYNSLILARHIAESRFGVATERPVVAAG
                     DVVAHLLREATCAPELWHQKAYLARVLTVSPEDGILDEGILPVVQALDGMTKDMIAMT
                     VEADGTGALYPVVYARRDGRLAEHALPGHPLLDFATRAHAEALQAAVGGLIGS"
     gene            complement(1762..2184)
                     /locus_tag="AC162A_00715"
     CDS             complement(1762..2184)
                     /locus_tag="AC162A_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR5009388.1"
                     /translation="MATREEIADILAGFTLFGDLQTPQLLGVAGVFDEVVFPPGERIL
                     RQGLTGSGFFVILDGTAEVRIDGQQRATLGRGDFFGEVSILLGEPPTADVVASTTLRC
                     IQIAGSAVEGFMVDHPKVMYRMLQAQARRLRNANRWRS"
     gene            2356..2784
                     /locus_tag="AC162A_00720"
     CDS             2356..2784
                     /locus_tag="AC162A_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR5009389.1"
                     /translation="MTLTQDRRSALIGGCPLFRGIEDTGLEALAAAAVEVDFPADRVI
                     ARQGEIGTGFFVIVEGMVRVSRDGNVVARLGPGEFFGELSILDGGPRIAQVAAEVPTR
                     CLALASWDFERVLREEPGVALSVLRVVAGRLREVTTDHRT"
     gene            2809..5526
                     /locus_tag="AC162A_00725"
     CDS             2809..5526
                     /locus_tag="AC162A_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5009390.1"
                     /translation="MAAPARPTGEVTFLFTDIEGSTRLVDALGTAAWRPILARHRELI
                     RAALAAEDGLEIGTEGDSFFAVFLDPAAAVRSVAIAQRALAAEAWPEGGEVRVRMGLH
                     TGIGELDADGSYVGHDVHRAARVSSAANGGQVLLSEVTSSLVEGHLAPGLSLRPLGAH
                     RLKDLRPERIAQLDIDGLPGDFPPIHSIDARPNNLPLELTSFVGRERELEETRALLGT
                     SRLVTLTGPGGTGKTRLALQVAALLVDEYPDGTWFVPLGTITDSALVMPAIARAIGIG
                     DDPSRSPIDVVGAELGSKNVLLVLDNFEQVRGAATDVGELLRRAGKVRILATSRAPLR
                     VSGEQEYPVPGLPSPPDVDRLGPLARERLPAGVRSGTPEALAAFESVRLFVARGGSVK
                     PGFAVTSSNAGDVAAIVAHLGGVPLAIELAAARLRFLTPAAIHERLEGRLDLPGAGAA
                     DVPERQRSLRGTIMWSYELLDPPACRLFQRLAVFMGGFDVARAEGVAGPASDLGIDVL
                     DGLASLVDQSLVRSDEIDGEPRFSMLEPIREYALERLEASGDIGAVAERHAKAFLALS
                     RELEPDLAGDRQRTALDRLELEHANLRAAIDWGDASSDAEVALGITAAIWRFWQKRGY
                     LREARARVAALIARPWFATAPAPLRARTHEVMGGIIYWHGLVDGSRPDYEAALAIWRE
                     IGDEREIANAAYNLSFCYTMGILGPLPPDAREQADALLAEALAIYQSLGDVSGEANVY
                     WGIGIQHYFAQDNAAAAQAFEAALALNRKVGDRTQEAWSLHQLGSARLKLGDTAVAHE
                     LLAEGLRMFAEAGDVAGVTLGLDHMAAVAVSEGDLPRAARLSGLARRIQASSGTGLAG
                     VVESGFEVDTRATASQALAADELARYEAEGSAMPLPEGVRYALGEIDFP"
     gene            5523..8213
                     /locus_tag="AC162A_00730"
     CDS             5523..8213
                     /locus_tag="AC162A_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5009391.1"
                     /translation="MTTALPEGIVTFLFTDIEGSTRLLHEVGDAAYGETLGIHRTIVD
                     AAAGEHGGVRFGSEGDAVFVAFASAAGAIRAAVAAQRALAAYAWRGGDVRVRMGIHTG
                     EVLVVAGDYVGIEVHRAARVAAAAHGGQIIVTDATRTLAGEPDGAISMRDAGEHRLKD
                     FARAERLYQVVADGLETAFPPLKTLDLTPNNLPPQLTTFVGRAEVDNAVSLLDRTRLL
                     TLTGPGGTGKTRLSLALAGDCIERFPDGTWFVPLAPVTDPELVASAIAASIGLLAPQR
                     PPIERVKEHLRERTALLVLDNFEQVVSGAPIVADLLRSAPRLTVIVSSRAPLRISGEQ
                     EFPVPPLALPPAGTTDVETLLAAEAVRLFVERAMAVRPDFMLTPENGPHVAEVVRRLD
                     GLPLAIELAAARIRLLSPAAMAQRLGDRLGLLSAGARDLPERQRTLRGAIDWSHELLE
                     AEDRRLFARLGVFAGGGPLETAEAVCGIAGDEVPLDVLGGLERLAEQSLVRIGDDLHG
                     DVRFTMLETIREYALDKLAERGEADALRDRHADAFLAFATTSRPQAIDTTTAAAAHAR
                     LLDRLEDEHDNLRTALEHLTTAGDTGRASDLVFVLWRFWQMRGHLFEGRTRVDRVLAM
                     PGWTATPTRARLRTLEAAGGLAYWAADAESASAHYGAAVEMARLVGDDAELANALYNF
                     FFARRPAKDSEEWFQLIRDGDTALLDEALEIWTRLGDEHGMGRALWGLSEYHDYRGEA
                     ELGEAAATRALEIFERIGDPFWVSWSRFTRAFGRLLSGDVQRAAADLAPTVREFWASR
                     DLSGVTLVMSATAIMLLHEGRDADAYRVGGAERRLVAETGIHLASRVPLPGTADVDPD
                     TSDPRLQAALAEGASWTRDEAVDHVLAFNEALGREAPTRT"
     gene            8233..8448
                     /locus_tag="AC162A_00735"
     CDS             8233..8448
                     /locus_tag="AC162A_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009392.1"
                     /translation="MHAPDRAAAAASAQALRLVDLDPPRATTTDVEPDDPGELMLVCA
                     NCGARMDERKCKLICRCGYFLSCSDYY"
     gene            8474..>8884
                     /locus_tag="AC162A_00740"
     CDS             8474..>8884
                     /locus_tag="AC162A_00740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5009393.1"
                     /translation="MTAAGRPGSIGYRYFGRFQRDARLILVTSLVSGAALSLYWIDFN
                     LYLSSLGLTTATIGIVATIASVAGALVAFPASAASDRVGRRAVFAAGLVVGLLALVGL
                     IASEALPVIVVAAALWAAGNQAMQVVLSPYLTEHS"
BASE COUNT         1218 a         3208 c         3193 g         1265 t
ORIGIN      
        1 ctcgagctcg gcgcacagcg cgcggagcca gtcgctggag cccgggtccc gccgcgagtc
       61 gacgacgtcg ccgaacagga cggtcccggc agagcgggca gtcatccggc cgagcctact
      121 cgatggcaag ttagtcgtaa agggctaacc gctagataag tagccggtta tgactatcgt
      181 cggccctgtc aatcagccga cagccacggt ccacgctcag cccgctcgcc cggcgttggc
      241 gaactcagct cccgatcagc ccaccgaccg cggcctgcaa agcctcggcg tgggccctcg
      301 tcgcgaagtc cagcagcggg tggccgggca gcgcgtgctc ggcgagccgg ccgtcgcgcc
      361 gggcgtagac gacgggatag agcgctccgg tcccatcggc ttccacggtc atcgcgatca
      421 tgtccttggt catgccgtcg agggcctgga cgacgggcag gatcccctcg tcgaggatcc
      481 cgtcctccgg cgacaccgtg agcacgcggg cgagataggc cttctggtgc cacaactccg
      541 gggcgcaggt cgcctcgcgg agcagatgag cgacgacgtc gccggcggcc accacgggac
      601 gctccgtcgc caccccgaac cgcgactcag cgatgtgccg cgccaggatc agcgagttgt
      661 agcgatggcc ctgtacggcc cccgagtagg ccgggatgcc gtgcttcttg aggcccggcg
      721 cggccgagct cacggtcccc gcgaagtaga tgccgggcac cgaggccgac tcccacatcg
      781 gggtcacggc cggcagcttt gcctggccga acgtcgtgac gccgaggtcg gtcaggtcct
      841 ggagcgggca ggtgaaccca gtcgccgcga tgacctcgtc cgcatcgacg ctcatcgact
      901 ccccgttgtc ggtccgcttg agcatgacgc gatagtcatc gccgacgtgc tcgatcgagt
      961 cgatcgacgc gtcgaggatc gacaccccga gacccaggaa gttgtcctcg aacggctgga
     1021 catagcgcgc gcgcacgccg accagcgact tcgtctgcac cgaggtcttc gccggcgacg
     1081 gcgatgcgac cgtgatcttc gacgcccacg cagcaaggcc ggaggcgagc tcgaaccccg
     1141 agttctgctt gccgatgatc aacaggcgct tgcccgcgta ggtcgacgcc tcgcgggtct
     1201 ccgcgtagtg gcgcgcgtgc tcgatgcccg gctgcttcgg cgtccacggc tgcgcgatgc
     1261 cgacggccag cacgagcgtc cggcagcggt actcgccgtc gctcgtctcg agggtgaaga
     1321 cggggccgtc gggtccatcg tcgcgccggg tccgctccca gcggcagtcg taacgcaccg
     1381 cgagcccggc gcggtcggcg aaggccgcga ggctcgcctc catctcgggc cgcgacggga
     1441 agtacgacga gccgtccatg aaccccgcct gcagagaccg cagctctggc tcgaaggcga
     1501 tcaggctgtt ccagtcgtag cgctggtact cgcgggccgt cgcgagctcg ggcgcgtacg
     1561 gcttggtcca cgacagcagc cgctggaaga acggccagcg gcggaacatg ccgcccggcg
     1621 acggatccgc cgagatcacc gcatgcggca cgccgtagtg cttgaggccg tacgaggtct
     1681 gcagcccgcc ggggccgctg ccgacgacga tggcggggta ctccccgggt gggaagggac
     1741 gctcgtccgg ggccacgcgg ttcagctccg ccagcggttc gcgttgcgca gccgccgcgc
     1801 ctgcgcctgg agcatccggt acatgacctt ggggtggtcg accatgaagc cctcgaccgc
     1861 cgagccggcg atctggatgc agcgcagggt cgtcgacgcc acgacgtcgg ccgtcggcgg
     1921 ctccccgagc aggatcgaca cctcgccgaa gaagtcccct cgacccagcg tcgcccgctg
     1981 ctggccgtcg atccgcacct ccgcggtccc atcgaggatg acgaagaacc ccgagccggt
     2041 caggccctgg cgcaggatcc gctcgccggg cggaaacacg acctcgtcga acacgcccgc
     2101 gacgcccagg agctgcgggg tctggaggtc gccgaacagc gtgaatcccg cgaggatgtc
     2161 cgcgatctct tcacgcgtgg ccatccgccc ctgcctccat caacgttccg gggcgcctcg
     2221 ccccgacgcc ctcgccccac cgttggacga atgctatgcc acccggccgg ttcgcatcgg
     2281 atcgtgagcg ggtgttccgt tgacgacgaa ctcggaagcg gcgcaccatg acctccccag
     2341 aaccccggag gaccgatgac cctgacccag gaccggcgca gcgccctgat cggcggctgt
     2401 ccgctgttca gggggatcga ggacaccggc ctcgaggcgc tggccgccgc cgccgtggag
     2461 gtcgactttc cggccgatcg cgtgatcgcg cggcagggcg agatcggcac cgggttcttc
     2521 gtcatcgtcg agggcatggt ccgcgtcagc cgcgacggca acgtcgtcgc ccgcctcggc
     2581 ccgggcgagt tcttcggcga gctgtcgatc ctcgacggcg ggccgcggat cgcgcaggtc
     2641 gccgccgagg tgccgacgcg ctgcctcgcg ctcgcgtcgt gggacttcga gcgcgtgctg
     2701 cgcgaggagc cgggggtggc tctgtcggtc ctgcgggtcg tcgccgggcg cctccgcgag
     2761 gtgacgacgg accaccggac ctaggccgag cgatcgcagg acgtgaccgt ggctgcccct
     2821 gcccgcccga ccggcgaggt caccttcctg ttcacggaca tcgagggttc gacgcgcctc
     2881 gtcgacgcgc tcgggaccgc cgcgtggcgc ccgatcctcg cccgtcaccg ggagctgatt
     2941 cgcgccgcgc tggccgcgga agacgggctc gagatcggca ccgagggcga ctcgttcttc
     3001 gccgtgttcc tcgacccggc ggcggccgtg cgctccgtcg ccatcgcgca gcgggcgctg
     3061 gcggcggagg catggcccga gggcggtgag gtccgtgtcc ggatgggcct gcacacgggc
     3121 atcggcgagc tcgacgccga cggcagctac gtcgggcacg atgtccaccg ggccgcacgg
     3181 gtctcatcgg ccgcgaacgg cggccaggtg ttgctgtcgg aggtgacctc gtcgctggtc
     3241 gaggggcacc tggcgccagg tctcagcctc cggccgctgg gggcgcatcg cctcaaggac
     3301 ctgcgcccgg aacggatcgc ccagctcgac atcgacggac tgccgggcga ctttccgccg
     3361 atccactcga tcgacgcgcg ccccaacaac ctgccgctgg agctgacctc gttcgtgggt
     3421 cgcgagcgcg agcttgaaga gacgcgggcg ctgttgggaa cgtcacggct cgtgaccctg
     3481 acgggtcccg gggggaccgg caagacgcgg ctcgcgctcc aggtcgccgc gttgctcgtc
     3541 gacgagtacc ccgacggcac ctggttcgtc ccgctcggaa ccatcaccga ctcggcgctg
     3601 gtcatgcccg ccatcgcgcg ggccatcggc atcggcgacg acccgagccg ctcaccgatc
     3661 gacgtcgtcg gggcggagct cgggtcgaag aacgtcctgc tcgtcctcga caatttcgag
     3721 caggtgcgtg gggcagcgac ggacgtgggc gagctcctca ggcgcgccgg caaggttcga
     3781 atcctcgcga cgagccgcgc tccgctgaga gtgtccggcg agcaggagta ccccgtcccg
     3841 ggcctcccgt cgcctcccga cgtcgatcga ctcggcccgc ttgcgcgcga gcgccttccg
     3901 gccggcgtcc gatcggggac accggaggcg ctggcggcct tcgagtcggt ccgcctgttc
     3961 gtcgcgcgcg gcggctccgt gaagcccggc ttcgccgtca ccagctccaa tgccggggac
     4021 gtggccgcga tcgtcgcgca cctcgggggc gtgccgcttg cgatcgagct cgccgcggct
     4081 cgcctgcgct tcctcacgcc agccgcgatc cacgagcggc tggagggccg gctcgacctg
     4141 cccggagccg gagcggctga cgtcccggag cgccagcgct cgctgcgcgg cacgatcatg
     4201 tggagctacg agctgctcga cccgcctgcc tgccgcctgt tccagcggct ggccgtgttc
     4261 atgggcgggt tcgacgtcgc gcgggccgag ggagtcgccg ggccggcgtc ggacctgggc
     4321 atcgacgtgc tcgacggcct tgcctcgctc gtggaccaga gtctcgtccg cagcgacgag
     4381 atcgatggcg agccgcggtt ctcgatgctc gagccgatcc gggagtacgc gctcgagcgc
     4441 ctggaggcgt cgggcgacat cggcgcggtc gcagaacgcc acgccaaggc ctttctcgcc
     4501 ctgtcccgcg agctcgagcc ggacctcgcg ggcgaccgcc agcgaacagc cctcgaccgg
     4561 ctggaactcg agcacgcgaa cctgcgcgcg gcgatcgact ggggcgacgc cagcagcgac
     4621 gccgaggtcg ccctcgggat caccgccgcg atctggcggt tctggcagaa gcgcggctac
     4681 ctgcgcgagg cacgcgccag ggtcgcggcg ctgatcgccc gaccctggtt cgccaccgcg
     4741 ccggccccgc tgcgcgcgag gacgcacgag gtgatgggcg ggatcatcta ctggcacggg
     4801 ctggtcgacg gatcgcgccc cgactacgag gctgcgcttg cgatctggcg cgagatcggc
     4861 gacgagcgag agatcgcgaa cgcggcctac aacctctcgt tctgctacac gatgggcatc
     4921 ctcgggccgc tgccgccgga cgctcgcgag caggccgacg cactgcttgc cgaggcgctc
     4981 gcgatctacc agtcgctggg cgacgtctcc ggagaggcga atgtctactg gggaatcggc
     5041 atccagcact acttcgccca ggacaacgcg gcggcggcgc aggcgttcga ggcagcgctg
     5101 gcgctgaacc gcaaggttgg cgaccggacc caggaggcct ggtcgctgca ccagctcggg
     5161 tcggcccgcc tcaagctcgg tgacaccgcc gtcgcgcacg agctgctcgc ggaagggctg
     5221 cgcatgttcg ccgaagccgg cgacgtcgcc ggcgtcacgc tcgggctcga ccacatggcc
     5281 gccgtcgcgg tcagcgaggg cgacctgccc cgggcggccc gcctgtcggg cctcgcccgg
     5341 cgcatccagg cgtcgtcggg gacgggtctc gcgggcgtcg tcgagagtgg gttcgaggtc
     5401 gacacgcggg cgaccgcgtc gcaggcgctc gcagccgacg agctcgcacg ctacgaggcc
     5461 gaagggtccg ccatgcccct gcccgagggc gtgcgctacg ccctgggcga gatcgacttc
     5521 ccgtgacgac cgcgctgccc gagggcatcg tcacgttcct gttcaccgac atcgagggct
     5581 cgacccggct gctccatgag gtcggcgacg cggcgtatgg cgagacgctg gggatccacc
     5641 ggaccatcgt cgatgccgcg gccggcgaac atggcggcgt caggttcggg tccgagggcg
     5701 acgcggtgtt cgtcgcgttc gcgtcggccg cgggcgcgat ccgggcggcg gtcgcggccc
     5761 agcgtgccct cgccgcatac gcgtggcggg gcggcgacgt ccgggtccgg atgggcatcc
     5821 acacgggcga ggtgctggtg gtcgccggcg actacgtggg catcgaggtg catcgcgccg
     5881 cgcgggtagc ggcggccgcg cacggcggcc agatcatcgt cacggacgca acgcggacgc
     5941 tggccggcga gccggacggt gccatctcca tgcgcgacgc gggcgagcac cggctcaagg
     6001 acttcgcgcg cgcggaacgc ctgtaccagg tggtggccga cgggctcgag accgcgtttc
     6061 ccccgctcaa gaccctcgac ctcacgccca acaacctgcc gccgcagctg acgacgttcg
     6121 tcggtcgagc cgaggtggac aacgccgtct cgctgctgga ccggacgcgg ctgctgaccc
     6181 tcaccgggcc cggaggaacg ggcaagacgc ggctctcgct ggcgctggcg ggcgactgca
     6241 tcgagcgctt tcccgacggg acatggttcg tgccgctcgc cccggtcacg gaccccgagc
     6301 tcgtggcctc ggccatcgcc gcatcgatcg gcctgctcgc tccgcaacgc ccaccgatcg
     6361 agcgcgtcaa ggagcatctc cgggagcgaa ccgcgctgct cgtgctggac aacttcgagc
     6421 aggtcgtgtc cggcgccccg atcgtcgccg acctgctgcg ctcggcgccc aggctcaccg
     6481 tcatcgtctc gagccgggcc ccgctgcgga tctccggcga gcaggagttc cccgtcccgc
     6541 ccctggcctt gccgccggcg ggcacgaccg atgtcgagac gctgctcgcg gccgaggccg
     6601 tgcgcctgtt cgtcgagcgg gcgatggccg tgcggccgga cttcatgctc acccccgaga
     6661 acggtcccca cgtggccgag gtcgttcgga ggctcgatgg cctgccgctc gcgatcgagc
     6721 tggccgcggc gcggatccgg ttgctttcgc cagccgcgat ggcacagcga ctgggggacc
     6781 gcctaggcct gctgtcggcc ggcgcgcggg atctgcccga gcggcagcgg accctccgcg
     6841 gcgcgatcga ctggagccat gagctcctgg aggcagagga ccgccggctg ttcgcccggc
     6901 tgggcgtatt cgcgggcggc ggtccgctcg agaccgcgga ggctgtgtgc gggatcgcgg
     6961 gcgacgaggt ccccctcgac gtcctcggcg gcctcgaacg gctcgcggaa cagagcctcg
     7021 tgcggatcgg cgacgacctc cacggcgacg tccggttcac gatgctcgag acgattcgcg
     7081 agtacgccct tgacaagctc gccgagcggg gcgaagccga cgccctccgg gaccgccacg
     7141 cggacgcctt cctcgcgttc gcgacgacgt cgcgaccgca ggcgatcgac acgaccacgg
     7201 cggccgcggc ccacgcgcgg cttctcgatc gtctcgagga cgagcacgac aacctccgga
     7261 cggcgctcga gcacctgacg acggccgggg acaccgggcg ggcgtcggac ctcgtgttcg
     7321 tcctctggcg gttctggcag atgcgcggcc acctcttcga gggccggacc cgggtggatc
     7381 gcgtgctcgc gatgcccggc tggaccgcga cgccgacacg agcccgtctg cggacgctcg
     7441 aggcggccgg cggcctggcg tactgggctg ccgacgccga atcggcgagc gcccactacg
     7501 gggcggccgt cgagatggcc cggctggtcg gcgacgatgc cgagctggcc aatgcgctct
     7561 acaacttctt cttcgcgcgc cggccggcca aggactccga agagtggttc caactcatcc
     7621 gcgacgggga caccgcgctc ctcgacgagg cactcgagat ctggacccgc ctcggcgacg
     7681 agcacgggat gggccgtgcg ctgtggggcc tgagcgagta ccacgactac cgcggcgagg
     7741 cggagctcgg cgaggcggcc gccacgcgcg ccctcgagat cttcgagcgc atcggtgacc
     7801 cgttctgggt gagctggtcg cggttcacgc gcgccttcgg ccggctcttg agtggcgacg
     7861 tccagcgggc cgcggcggat ctcgcgccca cggttcgcga gttctgggcg agccgcgacc
     7921 tctcaggtgt gacgctcgtg atgtccgcga ccgcgatcat gctgctgcac gagggtcgcg
     7981 acgccgacgc ctaccgggtc ggcggtgccg agcgacggct ggtggccgag accggcatcc
     8041 acctcgcgtc ccgcgtgccc ctgccgggaa cggcggacgt cgacccggac acgtccgacc
     8101 cccgcctcca ggccgccctg gccgaaggcg cgtcgtggac gcgcgacgag gccgtggacc
     8161 acgtgctcgc attcaacgag gcgctcggcc gcgaggcacc cacgcggacc tgagcggcgt
     8221 acgatggcgg gcgtgcacgc cccggatcgc gccgccgccg cggcatccgc ccaagcgctc
     8281 cggctcgtcg acctcgatcc gccgagggcc acgacgaccg acgtcgagcc agacgatccg
     8341 ggcgagctga tgctcgtgtg cgccaactgt ggcgcgcgca tggacgagcg caagtgcaag
     8401 ctcatctgcc gctgcgggta cttcctctcg tgctcggact actactgatc gcgcacgacg
     8461 atcccgggga ggcgtgacag cggccggccg gcccgggtcg atcggctacc ggtacttcgg
     8521 ccggttccag cgcgacgcgc ggctgatcct cgtcacgagc ctcgtctcgg gcgcggcgct
     8581 cagcctgtac tggatcgact tcaacctgta cctctcgtcg ctggggctga cgacggcgac
     8641 catcggcatc gtggcgacca tcgcgtccgt ggcgggggcc ctcgtcgcgt tcccggccag
     8701 cgccgcatcg gaccgggtcg gccggcgggc cgtcttcgcg gccgggttgg tcgtgggact
     8761 gctggccctc gtcgggctca tcgcgagcga ggccctgccg gtgatcgtgg ttgcggcggc
     8821 gttgtgggcg gcgggcaacc aggcgatgca ggtcgtgctg tcgccgtacc tcaccgagca
     8881 cagc
//
LOCUS       DBNHXO010000034         4450 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1198596, whole genome shotgun sequence.
ACCESSION   DBNHXO010000034 DBNHXO010000000
VERSION     DBNHXO010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1198596"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="AC162A_00745"
     CDS             <1..458
                     /locus_tag="AC162A_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR5009394.1"
                     /translation="DRNITVGQIYMLKLHHLVEDKIHARSTGPYSLITQQPLGGKAQF
                     GGQRFGEMEVWALEAYGAANILQELLTVKSDDVLGRVQTYEAIVKGEEIQPPRVPESF
                     KVLIKELRSLGLNAEILDQNDEEIPLAEDDASGYLLPDLGGINLAGFED"
     gene            626..>4450
                     /gene="rpoC"
                     /locus_tag="AC162A_00750"
     CDS             626..>4450
                     /gene="rpoC"
                     /locus_tag="AC162A_00750"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR5009395.1"
                     /translation="MLEVNNFSAIRISLASPDQIREWSKGEVTKPETINYRTLKPEKD
                     GLFDERIFGPTRDWECYCGKYKRIRYKGIICDKCGVEVTRSKVRRERMGHIQLASPVS
                     HIWYFKGTPSRLGILLDVSPRNLERILYFALYIVTHVDEEARQRALKALDEQSEGRGG
                     TGGQRLTELEEQLKADVNRQRDELRATLATTKGELEAQRTTRTEELVREAQAVEAALK
                     ALGTGAAEETIAFAPTGEVVVAAGDKGGKDATAKLRKIVAAETERVTEELQTREADEE
                     RQTEQKVQDLVAAMDEALRAERETLATDAQGQKDELRKAREEIESLKPLMTIGEIELR
                     SLDEKYGSGAKTGRIFWAGMGAEAVRDIIVRMDLEELSRQLHAEVRTSSGQRRKKAIK
                     RLRLIEAFRRSGNRPEWMILSVLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNN
                     RLKRLLELGAPEIIIRNEKRMLQEACDALIDNGRRGRAIAGTGNHRLKSLSDMLKGKQ
                     GRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKKMALELFKPFVMRQLVEKGFAHN
                     IKSAKRIVERVRPEVWDVLEDVIKDHPVLLNRAPTLHRLGIQAFIPVLVEGSAIQIHP
                     LVCTAFNADFDGDQMAVHVPLSTAAQEEARAMMLSTANLLSPADGSPVVAPTQDMVLG
                     CFYLTMDGPTVRGHKPRAFASEDEAIIAYELGARGIDHASRISSEREEGDVSPVKHRS
                     AKLLLHEPVDVAVLGWDAETEALTERVVRTTVGRVIFNRVLPDQMRFANDAMRRADLK
                     RLVDTCYRLLGPAETAHLVDGIKDVGFKFATRGGLTIGLWDIVVPKAKREKLIEADEA
                     VASIDKQFQRGLITEDERYEQVVELWQRVTKDVSDRMMESLGDDNPVRMMTDSGARGN
                     KGNIGQLGAMRGLMADPSGRIIDVPVRSNFREGMTVLEYFISTHGARKGLADTALRTA
                     DSGYLTRRLVDVAQDVITRQDDCGTEEGSWIIRAESAEEPDAFQRRLVGRLAAADLID
                     PKLKVKKGETAPIVVARGDMISEELARTIDAAGIDEVLVRSPLACESRYGVCRICYGR
                     NLATGEMIGIGEAVGIIAAQSIGEPGTQLTMRTFHTGGVAGQDITQGLPRVEELFEAR
                     IPKGKAEISHIDGVVEVIAGDTGRKVKVISREAFDTPLELTAGAELRAAPGDLVALNQ
                     VLARAATGDTLAPVAGFLAKTSDGIVVRGEDIVEREYSIPHSAKLLVEDGQEIRAGDA
                     ITDGPINPQEYLD"
BASE COUNT          795 a         1579 c         1503 g          573 t
ORIGIN      
        1 tcgatcgcaa catcacggtc ggccagatct acatgctcaa gctccaccac ctcgtcgagg
       61 acaagatcca cgcccggtcc acgggcccct acagcctgat cacgcagcag ccgctgggtg
      121 gcaaggcgca gttcggcggc cagcggttcg gcgagatgga ggtctgggcc ctcgaggcct
      181 atggcgccgc caacatcctc caggagctgc tcacggtgaa gtcggacgac gtcctcgggc
      241 gcgtccagac gtacgaagcc atcgtcaagg gcgaggagat ccagcccccg cgggtccccg
      301 agtcgttcaa ggtcctgatc aaggagctgc ggagcctcgg gctcaacgcg gagatcctcg
      361 accagaacga cgaggagatt ccgctggccg aggacgatgc ctccggctac ctgctccccg
      421 atctcggcgg gatcaacctc gccgggttcg aagactaacg aaatcctctc gccgagggaa
      481 cgcaggagct ttcctcggca atcggagggc tggtcgtaga cctgaccaat caatcaaaca
      541 gcgggaccgg cgtccggccg gcccggttcc gctcgccact acgcatgttc gcgccgcctg
      601 agccgcggcg gcgctgagga gaccgatgct cgaggtcaac aacttctcgg caatccgcat
      661 ctcgcttgcc agcccggacc agatccggga gtggtcgaag ggcgaggtca cgaagcccga
      721 gacgatcaac taccggacgc tgaagcccga gaaggacggt ctgttcgacg agcggatctt
      781 cgggcccacc cgcgactggg agtgctactg cgggaagtac aagcgcatcc gctacaaggg
      841 gatcatctgc gacaagtgcg gcgtcgaggt cacgcgcagc aaggtccgcc gtgagcgcat
      901 gggccacatc cagctggcca gccccgtcag ccacatctgg tacttcaagg gcacgcccag
      961 ccggctcggc atcctgctcg acgtcagccc ccgcaacctc gagcgcatcc tgtacttcgc
     1021 gctgtacatc gtcacccacg tcgacgagga ggcgcgccag cgggcgctca aggccctcga
     1081 cgagcagtcc gagggtcgcg gcggcaccgg cggccagcgc ctgaccgagc tcgaggagca
     1141 gctgaaggcg gacgtcaacc gccagcgcga cgagctgcgc gcgacgctcg ccaccaccaa
     1201 gggcgagctc gaggcccagc gcacgacccg caccgaagag ctggtccgcg aggcgcaggc
     1261 ggtcgaggcc gccctcaagg cgctcggcac cggcgcggcc gaggagacca tcgcgtttgc
     1321 cccgacgggc gaggtcgtgg tcgccgcggg cgacaagggc ggcaaggacg ccacggcgaa
     1381 gctgcgcaag atcgtcgccg ccgagaccga gcgtgtcacc gaggagctcc agacccgcga
     1441 ggcggacgag gagcgccaga cggagcagaa ggtccaggac ctggtcgcgg ccatggacga
     1501 ggcgctgcgc gccgagcgcg agacgctggc cacggacgcc cagggccaga aggacgagct
     1561 gcgcaaggcc cgcgaggaga tcgagtccct caagccgctg atgacgatcg gcgagatcga
     1621 gctccgctcg ctcgacgaga agtacggctc gggcgccaag accggccgga tcttctgggc
     1681 gggcatgggc gcggaggccg tgcgcgacat catcgtgcgc atggacctcg aggagttgtc
     1741 ccgccagctg cacgccgagg ttcggaccag ctccggccag cggcgcaaga aggcgatcaa
     1801 gcgcctgcgc ctgatcgagg cgttccgccg ctccggcaac cgcccggagt ggatgatcct
     1861 ctcggtcctg ccggtcatcc cgcccgacct gcgcccgatg gtccagctcg acggcggccg
     1921 gttcgcgacg tcggacctca acgacctgta ccggcgcgtg atcaaccgca acaaccgcct
     1981 caagcggctg ctggagctcg gcgcgcccga gattatcatc cgcaacgaga agcggatgct
     2041 ccaggaggcg tgcgacgccc tgatcgacaa cggccgccgc ggccgggcga tcgcgggcac
     2101 cggcaaccac cgcctcaaga gcctctcgga catgctcaag ggcaagcagg gccggttccg
     2161 ccagaacctg ctcggcaagc gcgtcgacta ctccggccgt tcggtgatcg tcgtcggccc
     2221 cgagctcaag ctgcaccagt gcggcctgcc caagaagatg gcgctcgagc tgttcaagcc
     2281 gttcgtcatg cgccagctgg tggagaaggg cttcgcccac aacatcaaga gcgccaagcg
     2341 tatcgtcgag cgcgtccggc ccgaggtctg ggacgtcctc gaggacgtca tcaaggacca
     2401 cccggtcctg ctgaaccgcg cgcccacgct ccaccgcctg ggcatccagg cgttcatccc
     2461 ggtgctcgtc gaaggctcgg cgatccagat ccacccgctc gtgtgcacgg cgttcaacgc
     2521 ggacttcgac ggcgaccaga tggccgtcca tgtcccgctg tccacagccg cgcaggaaga
     2581 ggcgcgtgcc atgatgctct cgacggcgaa cctgctgtcg ccggcggacg gctcgccggt
     2641 ggtcgcgccg acccaggaca tggtcctcgg ctgcttctac ctcacgatgg acggcccgac
     2701 ggtgcgcggc cacaagccgc gcgccttcgc gagcgaggac gaggcgatca tcgcctacga
     2761 gctcggcgcc cggggaatcg accacgcgag ccggatctcc agcgagcgcg aagagggcga
     2821 cgtgtcgccc gtcaagcacc gcagcgccaa gctgctgctc cacgagcccg tggacgtggc
     2881 ggtcctcggc tgggacgccg agaccgaggc gctcaccgaa cgcgtcgtgc gcacgaccgt
     2941 cggccgggtg atcttcaacc gggtcctgcc cgaccagatg cgcttcgcca acgacgccat
     3001 gcggcgtgcc gacctcaagc gcctcgtcga cacctgctac cggctgctcg gccccgctga
     3061 gacggcgcac ctcgtcgacg gcatcaagga cgtcggcttc aagttcgcca cccgcggcgg
     3121 gctcacgatc ggcctgtggg acatcgtcgt gcccaaggcc aagcgcgaga agctcatcga
     3181 agccgacgag gccgtcgcct cgatcgacaa gcagttccag cgcggcctca tcaccgagga
     3241 cgagcgctac gagcaggttg tcgagctctg gcagcgcgtc accaaggacg tctccgaccg
     3301 gatgatggag tcgctgggcg acgacaaccc ggtccggatg atgaccgact ccggcgcgcg
     3361 cggaaacaag ggcaacatcg gccagctggg cgccatgcgc ggcctgatgg ccgacccgtc
     3421 cggccggatc atcgacgtcc cggtccgcag caacttccgc gagggcatga cggtcctcga
     3481 gtacttcatc agcacccacg gcgcccgcaa gggcctcgcc gacaccgcgc tccgcaccgc
     3541 ggactccggg tacctcaccc ggcgcctcgt ggacgtcgcg caggacgtca tcacccgcca
     3601 ggacgactgc gggaccgaag agggcagctg gatcatccgc gccgaatccg cggaggagcc
     3661 cgacgcgttc cagcgccggc tcgtcggccg gctggccgcg gcggacctca tcgaccccaa
     3721 gctcaaggtc aagaagggcg agacggcgcc gatcgtggtc gcccgaggcg acatgatcag
     3781 cgaggagctc gcgcggacga tcgacgcggc cggcatcgac gaggtgctgg tccggtcacc
     3841 gctcgcgtgc gagtcgcgct atggcgtgtg ccgcatctgc tacggccgca acctcgccac
     3901 cggcgagatg atcggcatcg gcgaggcggt gggcatcatc gccgcccagt cgatcggcga
     3961 gcccggcacg cagctgacca tgcggacgtt ccacaccggc ggcgtcgccg gccaggacat
     4021 cacgcagggc ctgccgcggg tcgaggagct gttcgaggcc cggatcccca agggcaaggc
     4081 cgagatcagc cacatcgacg gcgtcgtcga ggtcatcgcg ggcgacaccg gccgcaaggt
     4141 caaggtcatc agccgcgagg cgttcgacac gccgctcgag ctcacggccg gtgccgagct
     4201 gcgggccgcc ccgggcgacc tcgtcgcgct caaccaggtg ctggctcgtg ccgcgacggg
     4261 cgacacgctg gcgccggtcg cgggcttcct cgccaagacc agcgacggca tcgtcgtccg
     4321 cggcgaggac atcgtggagc gcgagtactc gatcccgcac agcgcgaagc tcctggtcga
     4381 ggacggccag gagatccgcg ccggcgacgc gatcacggac ggcccgatca acccgcagga
     4441 gtacctcgac
//
LOCUS       DBNHXO010000035         2959 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1397298, whole genome shotgun sequence.
ACCESSION   DBNHXO010000035 DBNHXO010000000
VERSION     DBNHXO010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1397298"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2959
                     /locus_tag="AC162A_00755"
     CDS             <1..>2959
                     /locus_tag="AC162A_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5009396.1"
                     /translation="SAWLGVPLRADGRLIGLLVAQSYSSEHPYTRADLDLLAFVGQHV
                     ASALTRARAIEETRQRNAELAVINEIGDALARQLDFQAIVELVGERIRILFDSRSMFI
                     AIYDEPAGMIAYPYEIEDGQRYRSESIEFGPGLTSHVIRTRRPLRLHTSAESWAMGAI
                     DSDAVARETLPATGEGPESPGANAGIEAESWLGVPILIGERVIGVIAMEEFEKHAFDE
                     STERLLGTIATSMASALENARLFDETRRLLAETDERAAELAIVNEVQQGLAAQIEMQA
                     MYDLVGDRLRDVFDAQVLDIGILDREAGLIHFPYTIERGVRFPDEPIPLMGIRKHVIE
                     TREPLLMNERVAERAVELGQPGVLQGEEPKSTMWAPLLVGGEATGVVSVQNLDREQAF
                     SESDVRVLTTLAASLSVALENARLIHETRQRLTELATVNEIGQALASQLDLDPMYELV
                     GDLMRNTFAADLVYVAMHDVETNRIEFAYYSENGVTRDEEGFPYGQGLTSHIIRTREP
                     LLLNRDEAFEALEARMVGTPVKSYLGVPILLGDRAIGAISVQSMQEEGRFGAADVRLL
                     GTIAASVGVAIQNAQLFRDAGRRFDEMAALADAAREISATLEPTVVLERMTERARTLI
                     GVDTSAVYLADPDGQAFRAVVAIGAVADAIRADSIVLGEGIIGDVVAKRRAEIVNDVA
                     GDPRGVPIPGTSKEAEERLMAAPLLARDRVIGVMAVWRAAPGRPFTDADLAFFEGLAQ
                     QATIAIENARFYSDALEASRAAEDANQAKSTFLAAMSHEIRTPMNAIIGMSGLLLDTP
                     LDTEQRDYAETIKTSGDALLTVINDILDFSKIEAGKVELDAAPMELRRVVEGALDLLA
                     AEASAKDVELVYAVDDDLPAGIVGDAGRFRQIVINLLSNALKFTESGEVELRLGGSPI
                     AGPGRSAAHRWEIWVTVRDTGIGIPPEAMDRLFRSFSQVDVSISRRFGGTGLGLAISR
                     RLAELMDGSVTAES"
BASE COUNT          477 a         1029 c         1038 g          415 t
ORIGIN      
        1 agcgcgtggc tcggcgtccc actccgcgcc gacggccggc tcatcggcct gctcgtcgcg
       61 cagtcctact caagcgaaca cccctatacg cgcgccgacc tcgacctgct ggccttcgtc
      121 gggcagcacg tcgcctcggc gctgacgcgc gcgcgggcga tcgaggagac ccggcagcgc
      181 aacgctgagc tggcggtgat caacgagatc ggcgacgccc tcgcccggca gctcgacttc
      241 caggcaatcg tcgagctggt cggggagcgc atccggatcc tgttcgactc gcgctcgatg
      301 ttcatcgcga tctacgacga gccggccgga atgatcgcct acccgtacga gatcgaggac
      361 ggccagcggt accgcagcga atcgatcgag ttcggcccgg gcctcacgtc ccacgtgatc
      421 cgcacccggc gcccgctccg gcttcatacc agcgccgagt cctgggccat gggcgccatc
      481 gacagcgacg cggtcgcaag ggagacgctg ccggcgaccg gcgaggggcc cgagtcgccc
      541 ggcgccaacg ccggcatcga ggctgagtcc tggctcggcg tgcccatcct gatcggcgaa
      601 cgcgtgatcg gcgtcatcgc catggaggag ttcgagaagc acgccttcga cgagtcgacc
      661 gagcggctgc tcggcacgat cgcgaccagc atggcgagcg ccctcgaaaa cgcccggctg
      721 ttcgacgaga cgcggcggct gctcgccgag actgacgagc gtgccgccga gctcgcgatc
      781 gtcaacgagg tccagcaggg acttgccgcg cagatcgaga tgcaggcgat gtacgacctc
      841 gtcggcgacc ggctgcgcga cgtcttcgac gcccaggtcc tcgacatcgg gatcctcgac
      901 cgcgaggcgg gcctcatcca cttcccgtac acgatcgagc gcggcgtccg gtttcccgac
      961 gagccgatcc cactgatggg catccggaaa cacgtcatcg agacccggga gccgctgctg
     1021 atgaatgagc gggtggccga acgggcagtc gagctcggcc agccgggcgt cctccagggc
     1081 gaggaaccga agtccacgat gtgggcgccg ctgctcgtgg gcggcgaggc caccggcgtg
     1141 gtctcggtcc agaatctcga ccgcgagcag gccttctccg agtcggatgt ccgcgtcctg
     1201 acgacgctcg cggccagcct cagcgtcgcg ctcgagaacg cgcgcctgat ccacgagacg
     1261 cgccagcggt tgacggagct ggcaaccgtc aacgagatcg gccaggcgct ggcgagccag
     1321 ctcgacctgg atccgatgta cgagctggtc ggcgacctga tgcgcaacac gttcgcggcg
     1381 gacctcgtgt acgtggcgat gcacgacgtc gagaccaacc gcatcgaatt cgcctactac
     1441 agcgagaacg gggtgacccg ggacgaggag ggcttcccgt acggccaagg cctcacgtcg
     1501 cacatcatcc gcacccgcga gccgctgctc ctcaaccgcg acgaggcgtt cgaggcgctg
     1561 gaggcgcgca tggtcgggac gcccgtcaag tcctacctcg gtgtcccgat cctcctcggc
     1621 gaccgggcga tcggcgcgat cagcgtgcag agcatgcagg aggagggccg cttcggcgcg
     1681 gccgacgtcc gcctcctcgg cacgatcgcc gccagcgtcg gcgtcgcgat ccagaacgcc
     1741 cagctgttcc gggacgccgg gcgacggttc gacgagatgg cggccctcgc ggacgcggcg
     1801 cgtgaaatct cggccacgct cgagcccacc gtcgtgctgg agcggatgac cgagcgcgca
     1861 cgcacgctca tcggggtcga caccagcgcg gtctacctcg ccgatcccga cgggcaggca
     1921 ttccgcgccg tcgtcgccat cggggcagtc gcggacgcga tccgcgccga ctccatcgtg
     1981 ctcggagagg gcatcatcgg cgacgtcgtc gcgaagcggc gggcggagat cgtcaacgat
     2041 gtcgcggggg acccacgcgg ggtgccgatc ccgggcacga gcaaggaagc cgaggagcgg
     2101 ctgatggccg cgccgctgtt ggctcgcgac cgggtcattg gcgtgatggc cgtgtggcgt
     2161 gcggcgccgg gccgtccgtt caccgacgcc gacctggcgt tcttcgaggg cctcgcccag
     2221 caggcgacca tcgcgatcga gaatgccagg ttctactccg atgccctgga ggcgagccgg
     2281 gccgcagagg acgcgaacca ggccaagagc acgttcctcg cggcgatgag ccacgagatc
     2341 cggacgccca tgaacgcgat catcgggatg agcgggctcc tgctcgacac gccgcttgac
     2401 accgagcagc gcgattacgc cgagacgatc aagacctcgg gcgacgcgct gctcaccgtg
     2461 atcaacgaca tcctcgactt ctccaagatc gaggccggca aggtggagct ggacgcggcg
     2521 ccgatggagc tgcggcgggt cgtggaaggg gcgctcgacc tgctggcggc ggaggcctcg
     2581 gccaaggacg tcgagctcgt gtatgccgtc gacgacgacc tgccggccgg catcgtcggc
     2641 gacgccgggc ggttccgcca gatcgtgatc aacctgctgt cgaacgccct caagttcacc
     2701 gagtcgggcg aggtcgagct ccgcctcggt ggctcgccga tcgcgggccc gggccgctcc
     2761 gcggcccatc gctgggagat ctgggtgacg gtgcgcgaca caggcatcgg catcccgccc
     2821 gaggcgatgg atcgcctgtt ccggtcgttc agccaggtcg acgtgtcgat ctcccgccgg
     2881 ttcggcggga ccggcctggg gctcgcgatc agccggcggc tggccgagct gatggacggc
     2941 agcgtgaccg ccgagagca
//
LOCUS       DBNHXO010000036         3039 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3381692, whole genome shotgun sequence.
ACCESSION   DBNHXO010000036 DBNHXO010000000
VERSION     DBNHXO010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3381692"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1840)
                     /locus_tag="AC162A_00760"
     CDS             complement(<1..1840)
                     /locus_tag="AC162A_00760"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR5009397.1"
                     /translation="MADALYWIIPVAGIIAILFAIYLARDVLSRDQGTDAMQDVAGTI
                     LEGAVAFIRRQYTTIAIIAVFAAVIIGIVITLVETPAVADVPELTNPEWGAFAGLPIG
                     IMTAFAFLVGAACSMASGIIGMFVAVKANVRTASAARRSLVEAVQVAMRGGAVSGFLV
                     VALSLLGVWGIFTLYDTVVGDVTVAQAPFLIVGFGFGASFVALFAQLGGGIYTKAADV
                     GSDLVGKVEKGIPEDDPRNAGVVADLVGDNVGDCAGRGADLFESTAAENIGAMILGVA
                     VYSIAVAAGWPNPEAWIFFPLVIRAFGLLATIVAIFFVRGKETEDPMNMLNRGYWVTT
                     LLSAIGLAFVTAIMMQTNGGTGTGGLPTWTYFFAAGVVGLATSVAFVYITQFYTAGSF
                     RPVREIAEASKTGPATNIISGTAVGFETTLVTAVTISIALFLSYWLGNQAGLYSATGV
                     NVGGIFGTAVATMGMLMTTAYILAMDTFGPITDNAGGIAEFSRAEQGAREITDRLDAV
                     GNTTKALTKGYAIASASLAAFLLFSAYIDKINLIQGRRIEAGVPGAALLDSVNLANVN
                     VFIAALLAAMLVYFFSSLAIRAVGTTAQSIIVEVRRQFREMPGIMDY"
     gene            2100..2744
                     /gene="lepB"
                     /locus_tag="AC162A_00765"
     CDS             2100..2744
                     /gene="lepB"
                     /locus_tag="AC162A_00765"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR5009398.1"
                     /translation="MDQRSNFGCLVEVAETLILTLIIFFVIQNFIAQPYQVQQNSMER
                     TLEPGQYVLVDKLTPRWDSYSRGDVVVFNPPATWTSDPTPFIKRVIGLPGDMVAVKED
                     GLVYINDTPLDEPYTFANDAGVHEPTTALDEQSWVVPAGQLFVMGDHRQRSADSRVFG
                     PISTSDVIGRAFLRYWPISTFGILQTPSYPGVPSATGDAPKPGSSDPALQSAAP"
     gene            2767..>3039
                     /locus_tag="AC162A_00770"
     CDS             2767..>3039
                     /locus_tag="AC162A_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009399.1"
                     /translation="MTPLLVALAGLVAVGGIVAVSAREPRFAVLGVIIALVGAGYVAD
                     PLPSLAALAARLTGAVLGGYLIWVALRRAPAQTAGWHVGWPGAVALA"
BASE COUNT          500 a         1041 c         1053 g          445 t
ORIGIN      
        1 tgtaatccat gatcccgggc atctcgcgga actggcgccg gacctcgacg atgatcgact
       61 gggccgtcgt gcccaccgcg cggatcgcga gcgagctgaa gaagtacacg agcatcgccg
      121 ccagcagcgc cgcgatgaac acgttgacgt tggcgaggtt gaccgagtcg agcagcgctg
      181 cgcccgggac ccctgcctcg atgcggcggc cctggatcag attgatcttg tcgatgtagg
      241 cggagaacag caggaacgcg gccagcgacg ccgaggcgat cgcgtagccc ttggtcaggg
      301 ccttggtcgt gttgcccacg gcgtcgaggc gatcggtgat ctcgcgagca ccctgctcgg
      361 cgcggctgaa ctcggcgata ccgcccgcgt tgtcggtgat cggcccgaac gtgtccatgg
      421 ccaggatgta ggccgtggtc atcagcatgc ccatcgtcgc aacggcggtc ccgaagatgc
      481 caccgacgtt gacgcccgtg gcggagtaca gaccggcctg gttgccgagc cagtagctga
      541 ggaacagcgc gatgctgatc gtcaccgcgg tgacgagggt cgtctcgaag ccgaccgccg
      601 tgccgctgat gatgttggtg gccgggccgg tcttcgacgc ctcggcgatc tcgcgcaccg
      661 ggcggaacga gcccgcggtg tagaactgcg tgatgtagac gaacgccacg ctcgtcgcga
      721 ggccgacgac gccggccgcg aagaagtagg tccaggtggg caggccaccg gtgccggtgc
      781 cgccgttggt ctgcatcatg atcgccgtca cgaaggcgag cccgatcgcg gacagcaggg
      841 tcgtgaccca gtagccgcga ttgagcatgt tcatcgggtc ctcggtctcc ttcccgcgca
      901 cgaagaagat cgcgacgatc gtggccagca ggccgaacgc gcggatgacg agcgggaaga
      961 agatccaggc ctcggggttg ggccagccgg cggcgaccgc gatcgagtag accgcgacgc
     1021 ccaggatcat cgcgccgatg ttctcggcgg cggtcgactc gaacaggtcc gccccgcggc
     1081 cggcgcagtc gccgacgttg tccccaacga ggtccgcgac cacgcccgcg ttgcgggggt
     1141 cgtcctcggg gatgcccttc tcgaccttgc ccacgaggtc cgagccgacg tccgcggcct
     1201 tggtgtagat accgccgccc agctgagcga acagcgcgac gaacgaggcg ccgaacccga
     1261 agccgacgat caggaaggga gcctgcgcca cggtcacgtc gccgacgacc gtgtcgtaga
     1321 gcgtgaagat gccccacacg cccagcagcg agagggcgac cacgaggaac ccggagaccg
     1381 ccccgccgcg catcgcgacc tggaccgcct cgacgaggct gcgacgggcc gcggaggcgg
     1441 tccggacgtt ggccttgacg gcgacgaaca tgccgatgat ccccgacgcc atcgagcacg
     1501 ctgcgccaac caggaacgcg aacgcggtca tgatgccgat cgggaggccg gcaaaggcgc
     1561 cccactcggg gttggtcagc tccggcacgt ccgcgaccgc gggcgtttcg accagcgtga
     1621 tgacgatgcc aatgatcacg gccgcgaaca cggcgatgat ggcgatcgtc gtgtactggc
     1681 gccggatgaa ggcgaccgca ccctcgagaa tcgtgccggc gacgtcctgc atggcgtccg
     1741 tgccctggtc acgcgacagc acgtcgcgcg cgaggtagat cgcgaacagg atcgcgatga
     1801 tgcccgcgac cgggatgatc cagtagagcg cgtcggccat cagagtttgc agagcctcct
     1861 acggggattc gagacgccgg tgatctggtg tgcgggacct cgccgggtcg cggggcgggc
     1921 ggtctgaacg agcgcagcgg ctcgcgtgac gagggagggc aattaccggc gggggctcgg
     1981 aacgcagggg aacccgcgcg tcggcaggcg agagtgtatg cgtgggcgtt gacgggggtc
     2041 aaacgcagga acgggccgcg ggaccgcgtt ggacccgacc ctccctatac tccccgctcg
     2101 tggaccaacg atccaatttc ggatgtctcg tcgaagtcgc cgagaccctg atcctcacgc
     2161 tcatcatctt cttcgtcatc cagaacttca ttgcccagcc ctaccaggtc cagcagaact
     2221 cgatggagcg gaccctcgag ccagggcaat acgtgctggt ggacaagctc accccgcgct
     2281 gggactcgta cagtcgcggt gatgtcgtgg tgttcaaccc gcccgccacc tggacctccg
     2341 acccgacacc gttcatcaag cgcgtcatcg gcctcccggg cgacatggtg gccgtcaagg
     2401 aagacggcct cgtgtacatc aacgacacgc ccctcgacga gccctacacg ttcgcgaacg
     2461 acgccggcgt ccacgagccg acgacggccc ttgacgagca gagctgggtg gtcccggcgg
     2521 ggcagctgtt cgtcatgggc gaccaccgcc agcgatcggc cgactcgcgg gtgttcggcc
     2581 cgatcagcac gtcggacgtg atcggtcggg cgttcctgcg ctactggccg atctcgacct
     2641 tcggcatcct ccagacgccc tcgtacccgg gcgtgccgag cgccacgggc gacgcgccga
     2701 agccagggtc gtccgacccc gccctccagt ccgcggcgcc ctgacgcccc gggccgagcc
     2761 gatgccgtga cgcccctgct ggtcgcgctc gccggactcg tggccgtcgg cggcatcgtc
     2821 gccgtgagcg cccgtgagcc acggttcgcc gtcctgggcg tgatcatcgc gctggtcggc
     2881 gccggctacg ttgcggaccc actgcccagc ctcgccgcgc tcgccgctcg cctcaccggc
     2941 gccgtgctcg gcggctacct catctgggtc gcactgcgac gcgcgccggc gcagaccgcg
     3001 ggctggcacg tcggctggcc tggcgccgtg gcgctcgcg
//
LOCUS       DBNHXO010000037         7232 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1199486, whole genome shotgun sequence.
ACCESSION   DBNHXO010000037 DBNHXO010000000
VERSION     DBNHXO010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1199486"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..119
                     /locus_tag="AC162A_00775"
     tRNA            43..119
                     /locus_tag="AC162A_00775"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:77..79,aa:Val,seq:cac)
     gene            complement(331..732)
                     /locus_tag="AC162A_00780"
     CDS             complement(331..732)
                     /locus_tag="AC162A_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5009400.1"
                     /translation="MKKILVAYDGGDPARRALDLAIELAQKFGATVAVVSVTPVHPGR
                     APIDPWDDREVHLGELLEAQTILRAKGIEAEMLAPAGDPAITIERLAADGGFDAVVIG
                     SRGLGALARALQGSTSEHVATHSETTVIVAR"
     gene            complement(860..1909)
                     /locus_tag="AC162A_00785"
     CDS             complement(860..1909)
                     /locus_tag="AC162A_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019498419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HIR5009401.1"
                     /translation="MTVDLRTTYLGLALRSPIVASAAPHNGDPERARRLDQAGVGAIV
                     LPSLFEEEILAEELELGRVLEQGTEQFAESLGYFPRVDRFVGAADRYLTSLERVKAAV
                     GVPVVASLNASSPGGWVRHAQRIEAAGADALELNLYHLAVDGTRTATAMEAIDLELVA
                     AVRDAVTIPLAVKLSPYYSAFANFAPAVVTAGADGLVLFNRLYQPDLDLETLAVVPSI
                     ELSSPWEMRLPVRWIAILRPRLGAAVSLALTSGAHAGTDVVKGLMVGADVVMMTSALL
                     LHGPEHVATAEAELRAWMDEHEYESVAQLRGSASQANVEDPGAFERANYMQTLRSWTS
                     GSRSIASPAMRGGDG"
     gene            complement(1906..5517)
                     /gene="nifJ"
                     /locus_tag="AC162A_00790"
     CDS             complement(1906..5517)
                     /gene="nifJ"
                     /locus_tag="AC162A_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIR5009402.1"
                     /translation="MIERDQCLDGNEAVARVAYALSEVIAIYPITPASQMAEHCDDWA
                     SAGRPNQWGQVPAVVEMQSEAGAAGALHGALQTGTLATTFTASQGLLLMIPNMFKIAG
                     ELTPAVIHVAARTIATHALSIFGDHSDVMHARTTGWAMLSAGSVQEAHDFALVAHAAT
                     LRARVPFLHFFDGFRTSHEINRIVVLGDDDVRALVREEDVLAFRASGLTPDAPVVRGT
                     AQNPDVFFQAREASNPFHLAVPGIVEQVLDELAARTGRRYGLVDYHGAPDAERVVVSM
                     GSANGAIEEAVDALNERGERVGMLRVRLFRPFPAAQLLAALPPTVRSIAVLDRTKEPG
                     AVGEPLYLDVVGALSEAMDADAPRFAVPPRVIGGRYGLASKEFTPSMVKPVFEALAEP
                     RPMRHFTVGIVDDVTHLSLPIDDAFRYRRPAGEVQAMFFGLGSDGTVGANKASVKIIG
                     ESTGLYAQGYFVYDSRKSGSITVSHLRFGPEPIRSTYLVEDADFVACHQFGLLERVNV
                     LERAKRGATFLLNAPYGPDEVWEHLPRDVQATIVAKDIDLWVIDALAVAAEAGTGSRI
                     NTVMQPCFFQLAGIVAPDQAVAGIKAFVQKTYGKRGAAIVERNFAAIDRSLERLARVE
                     VGAVGAVGRAATDGRHPSPDVPDFVTRFTSRLLAGQGDLLPVSALPVDGTFPTGTAAF
                     EKRAIAQEIPIWDPTICIDCGKCAMVCPHASIRMKVFPASAVEGAPSSFLHKEFRSRE
                     LPGHRLTIQVAPDDCTGCGVCVDVCPARSKTDTSHKAIEMAPAAAHRDLERERWDVFR
                     SIPALDRGLLPHDTIKGSQVLEPLFEFSGACGGCGETPYIRLVSQLFGDRMIVANATG
                     CSSIYGANLPTTPWTVNASGRGPAWSNSLFEDNAEFGLGMRLGIDAQQEQARRLLERL
                     GPRLDADLVDGLLTAVQDDEAGIELQRDRVARLRGALATIDGVDAAEARHLLALAGDL
                     VRRGVWIIGGDGWAYDIGFGGLDQVLSSGRNVNILVLDTEVYSNTGGQASKSTPRGAV
                     AKFAAAGKRTGKKDLGAIARSYGDVYVAQISMGANDAQTTKALIEADAWPGPSLVIAY
                     STCIAHGIDMATSMAHQKDAVRSGYWPLYRFHPSEVDGGHPFKLDSAAPTIPVAEFAA
                     TEARFAVLQRTHPERAAELASLAQADADERWRYYQGLAGIERTVPHIDHRAPEGPEA"
     gene            complement(5596..6651)
                     /locus_tag="AC162A_00795"
     CDS             complement(5596..6651)
                     /locus_tag="AC162A_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR5009403.1"
                     /translation="MRATVFHGPGDIRVDQVARPRPGVGEAVIRVTTTTICGTDVHIV
                     KGEYPVRPGLVIGHEPVGVIEELGPGVTGYELGQRVLVGAITPCGQCRACLSGKLSQC
                     GHGGGYEALGGWRFGNTIDGAQAEYLLVPSAQANLTPIPDGVTDEQVVLLADIASTGF
                     SGAESGGVRIGDAVVVFAQGPIGLCATAGAKLMGASLIIGVDSDPVRLEMSRRMGADV
                     ALDFTQVDVVAEVHRLTGGGADVTIEALGTQQTFESALRSLRPGGTLSSLGVYSGKLE
                     MPYDAFAAGLGDHRVVTTLCPGGKERMRRLIATVESGRFDPLPLITHRFALDDIVEAY
                     DLFSHRRDGVLKVAIHP"
     gene            complement(6849..>7232)
                     /locus_tag="AC162A_00800"
     CDS             complement(6849..>7232)
                     /locus_tag="AC162A_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5009404.1"
                     /translation="RMIAVNSAIEVDLSGQVVADSIGHRLYSGVGGQMDFIRGAALAA
                     EGRAIIALPSTAAAGTVSRITATLQDGAGVVTTRAHARIIVTEWGVADLYGRSLRERA
                     VALITIAHPDHREELMAAARRDRLL"
BASE COUNT         1034 a         2517 c         2613 g         1068 t
ORIGIN      
        1 ggccgcccga cggggtatca tgcgggtccc ctcggggacc tcgggcgatt agctcagtcg
       61 gtcagagcgc acggttcaca tccgtgaggt cactggttcg agcccagtat cgcccaccat
      121 cctgaccctc ccgaccacca gggccttcaa ggccctggtt tcgtatctgc accgagtcac
      181 aagccaaccg gcgggaccct tgcgggttcc gccggttgtc accgtccggt gtcgcggccc
      241 ggctggaagg tgagccggat cgctatccga gcaggtggtg gggtgccggc cggtggccga
      301 cgactctcgg tctcgcgggc acccgtcgac tcagcgggcg acgatgaccg tcgtctcgct
      361 gtgggtcgcg acgtgctcgg acgtgctgcc ctggagcgct cgcgccaggg cgccgaggcc
      421 acgggacccg atcacgaccg cgtcgaagcc gccgtccgca gcgagtcgct cgatggtgat
      481 cgccggatcg ccggcgggcg ccagcatctc cgcctcgatc cccttcgcgc ggaggatcgt
      541 ctgcgcctcg agcagctcgc ccaggtgcac ctcacggtcg tcccacgggt cgatcggggc
      601 ccgcccggga tggaccgggg tgacgctgac gacggcgacg gtggccccga acttctgggc
      661 cagctcgatc gcaaggtcga gggcgcgacg cgcgggatcg ccgccgtcat acgccacgag
      721 gatcttcttc atgacaggct cctctgcgct tgtcggccgc cggccgacgg tcgtgcaccg
      781 tgagcatggc tgccgcgtgc gcccacgacg acggccgcac gtcccgttcg cgggggtcgg
      841 atggcccgat cgacggggcc tacccgtcac caccacgcat ggccgggctc gcgatcgatc
      901 tcgacccgga cgtccaggac cgcagcgtct gcatgtagtt ggcacgttcg aaggcgcctg
      961 gatcctcgac gttcgcctga ctggcgctgc cgcgcagctg cgcgaccgat tcgtactcgt
     1021 gctcgtccat ccaggcccgc aactccgcct ccgcggtggc gacatgctcg ggcccgtgga
     1081 gcaggagggc cgacgtcatc atcacgacgt cggcaccgac catcagcccc ttgacgacgt
     1141 ccgtgccggc gtgcgcgccg gacgtgagcg cgagtgagac ggcggcgccg agccgtggtc
     1201 gcaggatggc gatccagcgg acgggcaggc gcatctccca ggggctgctg agctcgatgc
     1261 tgggcacgac ggcaagcgtc tcgaggtcga ggtccggctg gtagagccgg ttgaacagca
     1321 cgagtccgtc cgcgcccgcc gtcacgaccg ccggtgcgaa gttcgcgaag gccgagtagt
     1381 acgggctcag cttgaccgcg agcggaatcg tgaccgcgtc gcgcacggcc gcgaccagct
     1441 cgaggtcgat ggcctccatg gccgtggccg tccgcgtgcc atcgaccgca aggtggtaga
     1501 ggttgagctc gagcgcatcc gcgcccgcgg cctcgatccg ctgcgcgtga cggacccagc
     1561 cgcccggcga gctcgcgttg aggctggcga ccaccgggac gccgacggcg gccttcaccc
     1621 gttcgagcga ggtcaggtag cgatcggcag cgccgacgaa tcgatcgacg cgcggaaagt
     1681 agccgagcga ctccgcgaac tgctcggttc cctgttcgag cacgcgcccg agctcgagct
     1741 cctccgcgag gatctcctcc tcgaacagtg acggaaggac gatggcaccg accccggcct
     1801 gatcgaggcg gcgggcccgc tccggatcgc cgttgtgcgg cgcggccgac gcgacgatcg
     1861 gcgagcgcag cgcgaggccg aggtaggtcg tacgcaggtc gacggtcatg cctcggggcc
     1921 ctccggtgct cgatggtcga tgtgcggcac cgttcgctcg atcccggcga ggccctggta
     1981 gtagcgccac cgttcgtcgg cgtcggcctg ggccagcgat gcgagctcgg ccgcgcgttc
     2041 cgggtgcgtc cgctgcagga ccgcgaatcg cgcctcggtg gcggcgaact cggcgaccgg
     2101 aatggtcggg gcggcggagt cgagcttgaa cgggtggccc ccgtcgacct ccgaggggtg
     2161 gaagcggtac agcggccagt agccgctccg gacggcgtcc ttctggtgcg ccatcgacgt
     2221 cgccatgtcg atgccgtggg cgatgcaggt gctgtacgcg atcacgaggg acggtcccgg
     2281 ccaggcgtcg gcctcgatca gcgccttcgt cgtctgggca tcgttggcgc ccatcgagat
     2341 ctgggcgacg tacacgtcgc cgtaggagcg cgcgatggcc ccgagatcct tcttcccggt
     2401 ccgcttgcca gcggcggcga acttggcgac cgcgccgcgc ggcgtgctct tcgacgcctg
     2461 gccgccggtg ttcgagtaga cctcggtgtc gaggacgagg atgttgacgt tgcggccgga
     2521 cgagagcacc tggtcgaggc cgccgaaccc gatgtcatag gcccagccgt cgccaccgat
     2581 gatccacacc ccccgtcgga cgaggtcgcc ggcgagcgcg agcaggtgtc gcgcctcggc
     2641 cgcgtcgacg ccgtcgatcg tcgccagcgc cccgcgcagg cgggccacgc ggtcacgctg
     2701 gagctcgatc ccggcctcgt cgtcctggac ggccgtcagc agcccgtcga cgaggtccgc
     2761 gtcgaggcgc gggcccaggc gctcgagcag gcggcgggcc tgctcctgct gggcgtcgat
     2821 cccgagccgc atcccgagcc cgaactcggc gttgtcctcg aacagcgagt tgctccaggc
     2881 cgggcctcgc ccgctggcgt tgacggtcca cggggtcgtg ggcaggttgg cgccgtagat
     2941 cgaggagcag ccggtcgcgt tggccacgat catccgatcg ccgaacagct ggctcaccag
     3001 gcggatgtac ggcgtctcgc cgcaccctcc gcatgcaccc gagaactcga acagcggctc
     3061 cagcacctgt gatcccttga tcgtgtcgtg cggcagcagt ccacggtcga gcgccgggat
     3121 cgaacggaac acgtcccacc gctcgcgctc gaggtctcga tgcgcggcgg caggcgccat
     3181 ctcgatcgcc ttgtggctgg tgtcggtctt gctgcgcgcc ggacagacgt ccacgcacac
     3241 gccgcacccg gtgcagtcat cgggcgcgac ctggatcgtc agccgatgcc cgggcagctc
     3301 gcgggagcgg aactccttgt gcaggaacga gctcggcgcg ccctccaccg ccgaggcggg
     3361 aaagaccttc atccggatcg aggcgtgcgg gcagaccatc gcgcacttgc cgcagtcgat
     3421 gcagatcgtc ggatcccaga tcgggatctc ctgggcgatc gcgcgcttct cgaaggccgc
     3481 ggtcccggtc gggaacgtgc cgtcgaccgg gagcgcgctg accggcagca ggtcgccctg
     3541 gccggcgagg agtcgtgacg tgaagcgggt gacgaagtcc ggcacatcgg ggctcgggtg
     3601 ccggccgtcg gtggccgccc tgccgaccgc gccgaccgcg ccgacctcga cgcgcgcgag
     3661 ccgctcgagg gaccggtcga tcgccgcgaa gttgcgctcg acgatggctg cgccgcgctt
     3721 gccgtaggtc ttctggacga acgccttgat gcccgcgacg gcctggtcgg gcgccacgat
     3781 tccggcgagc tggaagaagc acggctgcat gaccgtgttg atccggctgc cggtgccggc
     3841 ctcggcggcg acggcgagcg catcgatgac ccacaggtcg atgtccttcg ccacgatcgt
     3901 cgcctggacg tcccgcggca ggtgctccca cacctcgtcc gggccgtacg gggcgttgag
     3961 caggaacgtg gcgccgcgct ttgcgcgctc gaggacgttc acccgctcca gcaggccgaa
     4021 ctggtggcag gcgacgaagt cggcgtcctc cacgaggtag gtggagcgga tcggctcggg
     4081 gccgaagcgc agatgagaga cggtgatcga tccggacttg cgcgagtcgt acacgaagta
     4141 gccctgggcg tagaggccgg tgctctcgcc gatgatcttg accgaggcct tgttcgcacc
     4201 gaccgtgccg tccgagccca atccgaagaa catggcctgg acttcgcccg ccgggcgtcg
     4261 gtaccggaag gcgtcgtcga tcggcaggct gagatgcgtc acgtcgtcga cgatgccgac
     4321 cgtgaagtgg cgcatcggtc gcggctcggc cagcgcctcg aacacgggct tcaccatcga
     4381 gggcgtgaac tccttcgacg cgagcccgta gcgtccgccg atcactcgcg gcggaaccgc
     4441 gaagcgcggc gcatcggcat ccatcgcctc gctgagggca ccgacgacgt cgagatacag
     4501 cggctcgccg accgcgcccg gctccttggt ccgatcgagg acggcgatcg agcgcacggt
     4561 cggaggcagc gctgccagga gttgggccgc cgggaacggt cggaacaggc ggacccgcag
     4621 cattccgacg cgctcgccgc gctcgttgag ggcgtccacc gcctcctcga tcgcgccgtt
     4681 agccgagccc attgagacga ccacgcgttc cgcgtcgggt gccccgtggt agtcgaccag
     4741 gccgtaccgc cgccccgtcc gcgcggcgag ctcatcgagg acctgttcca cgatcccggg
     4801 tacggcgagg tggaacgggt tggacgcctc acgcgcctgg aagaacacat cggggttctg
     4861 ggccgtgccc cgcacgaccg gggcgtcggg tgtcagccca ctggcgcgga acgcgaggac
     4921 gtcctcctcg cggacaaggg cacggacgtc gtcgtcgccg aggaccacga tgcggttgat
     4981 ctcgtgggac gtccggaagc cgtcgaagaa gtggaggaac ggcacccgtg cgcggagggt
     5041 cgcggcatgc gccaccagcg caaagtcgtg cgcctcctgg accgacccgg cggacagcat
     5101 cgcccacccg gtcgtgcgcg cgtgcatcac gtcgctgtgg tcgccgaaga tcgacagcgc
     5161 gtgggtcgcg atcgtccgtg ccgcgacgtg gatcaccgcg ggggtgagct cgccggcgat
     5221 cttgaacatg ttggggatca tcaggagcag gccctgggag gccgtgaagg tcgtggccag
     5281 ggtcccggtc tgcagggctc cgtggagggc tcccgccgcc cccgcctcgg actgcatctc
     5341 gacgacggcc ggcacctggc cccactggtt gggccggccc gcggaggccc agtcgtcgca
     5401 gtgctcggcc atctgggatg ccggggtgat cggatagatc gcgatcacct cgctcagcgc
     5461 gtacgcgacg cgcgcgaccg cctcgttgcc gtccaggcac tggtcgcgct cgatcatcaa
     5521 gtcttccttc ctgtggtccc cgcttggtgg cgggctcgcc ggtggaaccg gcgagcccgt
     5581 accctcctcc gcggcttacg ggtggatcgc gaccttcagc acgccgtcgc ggcgatggct
     5641 gaacaggtcg tacgcttcga cgatgtcgtc gagcgcgaac cggtgggtga tcagcggcag
     5701 cgggtcgaag cgtcccgact cgacggtcgc gatgagccgt cgcatccgtt ccttgccgcc
     5761 cgggcacagc gtcgtgacga cgcggtgatc gccgaggccg gccgcaaacg cgtcatacgg
     5821 catctcgagc ttgcccgagt acacgccgag gctcgaaagg gtgccgcccg gacgcagcga
     5881 gcgcagggcg ctctcgaacg tctgctgggt cccgagggcc tcgatcgtca cgtccgcgcc
     5941 gccaccggtg aggcggtgga cctcggcgac gacgtcgacc tgggtgaagt cgagggccac
     6001 gtcggctccc atgcgacggg acatctcgag ccgcaccggg tcgctgtcga cgccgatgat
     6061 cagcgacgcg cccatgagct tggcgccggc cgtggcgcac aggccgatcg gcccctgcgc
     6121 gaacaccacg accgcgtcgc cgatgcgcac gccgcccgac tcggccccgg agaagccggt
     6181 cgaggcgatg tcggcgagga ggacgacctg ctcgtcggtg acgccgtcgg ggatcggggt
     6241 gaggttggcc tgggctgacg ggacgaggag gtactccgcc tgtgccccgt cgatggtgtt
     6301 gccgaagcgc cagccgccca gcgcctcgta gccgccgccg tggccgcact ggctgagctt
     6361 gccggacagg cacgcccggc actgtccgca aggggtgatg gcgccgacca ggacccgctg
     6421 gccgagctcg tagcccgtga cacccgggcc gagctcctcg atgacgccga cgggctcatg
     6481 gccgatcacg agcccggggc ggaccgggta ctcgcccttg acgatgtgga cgtccgtgcc
     6541 gcagatggtc gtcgtggtga cgcggatcac ggcttcgccc acccctgggc gcggtcgggc
     6601 cacttggtcg acgcggatgt cgccggggcc gtggaagacg gttgcgcgca tcacgcccga
     6661 ctcgacgggc ggaagcagcg gggtggcggt tgcgagggtc acggcggacc tccagtacgg
     6721 agcgttcgaa gccggcgctg gctcgccgat ccgtggcgag cgcgtgtcgc ttcaccgcca
     6781 tcgtcgggag cgtgggctcc gggcaacagg gccggaggtc acgcgcattc gggaccctgt
     6841 ggcgatagct agagcaggcg gtcgcgccgg gcagccgcca tgagctcctc gcggtgatcc
     6901 ggatgggcga tcgtgatcag ggccacggcc cgttcccgca agctccggcc gtacaggtcg
     6961 gccacgcccc actcggtgac gatgatccgc gcgtgcgccc gggtcgtgac gacgccggcg
     7021 ccgtcctgca gggtcgccgt gatccgcgag acggttccag cggccgccgt cgagggcagc
     7081 gcgatgatcg cccgcccctc ggccgcgagc gcggccccgc ggatgaagtc catctggccg
     7141 ccgacgcccg agtacaggcg atggccgatc gagtcggcga cgacctggcc gctgaggtcc
     7201 acctcgatcg ccgagttgac cgcgatcatc cg
//
LOCUS       DBNHXO010000038         2845 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2786772, whole genome shotgun sequence.
ACCESSION   DBNHXO010000038 DBNHXO010000000
VERSION     DBNHXO010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2786772"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="AC162A_00805"
     CDS             <1..505
                     /locus_tag="AC162A_00805"
                     /inference="COORDINATES: protein motif:HMM:NF015648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR5009405.1"
                     /translation="ALRFRGEEYVITSRRVIHVEGVINKRATDASLDRINDAVLTVSG
                     LGRIFGFGNLDVVTASGIGMERLQMLRDAKSFKKALIEAKHELEVDIARPTTPPLRPV
                     EPAAVVAMPPSPPAPTPTLPPPPPPGESKPADVVAELDRLAARHAAGEISTEAFEASK
                     RALLDRL"
     gene            556..1188
                     /locus_tag="AC162A_00810"
     CDS             556..1188
                     /locus_tag="AC162A_00810"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR5009406.1"
                     /translation="MFNPIELVPELITVAIFLLIAFPVHEFAHAAVAYLQGDATAKLF
                     GRLTLNPIVHFDPMGGLMTIVSVTLTGFIVGWAKPTPVNPANLHDRRNGEVFVALAGP
                     ASNFLMALLGAFVIRILLAAGVDLSGLPGFVAYNFVLFNVLLAIFNFIPVPPLDGSAL
                     LYRFLSPRQAWQVRPFLTQYGIFIVLGVVLLLSRQLGGLIYGITDVLVGA"
     gene            1169..1657
                     /locus_tag="AC162A_00815"
     CDS             1169..1657
                     /locus_tag="AC162A_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009407.1"
                     /translation="MSWWARKARQFRLHLVARVSPAERAALTAWLHPPELDLFDAMHV
                     ADRRHGLDVVAALRRSGVTDRDVLAAGLLHDCAKGDTGVGPRVAWSLGEAYGPRVLAL
                     AARVPGWGVALDRLRHHAEASASMVEAAGLPPFAVDLVRYQSEPRDPEFGRVFQLADE
                     AA"
     gene            1657..2526
                     /locus_tag="AC162A_00820"
     CDS             1657..2526
                     /locus_tag="AC162A_00820"
                     /inference="COORDINATES: protein motif:HMM:NF014654.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR5009408.1"
                     /translation="MALSGMPSVAFGDGGRPEAATRVTLPAFDGPLALLLSLIEARQL
                     DVLTVPLGALADAYLDALATLDEDRMGHVSAFVAVASQLILIKSRALLPRRAVDGPVA
                     LADEGPDPEAELRARLILYRAYRDAGTSLLDAATARGALFHRESVTARAAGLAGARAP
                     AAEPLSPAILVLALDGLARIAPPPAPPPETIRRTVTLTSRAAIIREALREAPAVVLQD
                     LLKGVRDRVVVAVTFLALLELMKRREIVVEQAQPWGPIVARRTTAAERAASGSQAIVD
                     EDTPLDESLESFA"
     gene            2523..>2845
                     /locus_tag="AC162A_00825"
     CDS             2523..>2845
                     /locus_tag="AC162A_00825"
                     /inference="COORDINATES: protein motif:HMM:NF016005.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR5009409.1"
                     /translation="MTELEAVGPGELAAGEADDPTTAGELTEAQLEALLFVAEKPLSR
                     REISLLAGVDRETVDALLGDLEVSLDGRGIRLVLSGDHVELTTAPDAGALIARYVGAD
                     AVRLSP"
BASE COUNT          342 a         1071 c         1003 g          429 t
ORIGIN      
        1 cgcgctgcgg ttccggggcg aggagtacgt catcacgagc cgccgcgtca tccacgtgga
       61 gggcgtgatc aacaagcgtg cgaccgacgc ttcgctcgac cggatcaacg acgcggtgct
      121 cacggtgtcg gggctcgggc ggatcttcgg cttcggcaac ctcgacgtcg tcacggcatc
      181 ggggatcggg atggagcggc tgcagatgct gcgcgacgcc aagtcgttca agaaggcgct
      241 gatcgaagcc aagcacgagc tcgaggtcga catcgcgcgc ccgacgacgc cgccgctgcg
      301 gcccgtcgag ccggctgccg ttgtcgccat gccaccctcg cctcccgcgc caacgcccac
      361 gttgccgcct ccgccgccgc ccggagagtc gaaacccgcc gacgtcgtgg ccgagctcga
      421 ccggctcgcc gcccggcatg cggccgggga gatctcgacc gaggccttcg aggcgtccaa
      481 gcgagcgctg ctcgaccgcc tctgaccgcg cggccggtac gatacgggcg acttccgcca
      541 ccacccgagg cctgagtgtt caatccgata gagcttgttc ccgagctcat caccgtcgcg
      601 atcttcctgc tgatcgcatt cccggtccac gagttcgcgc acgccgccgt ggcctacctc
      661 cagggcgacg cgacggccaa gctgttcggg cgcctgacgc tgaacccgat cgtccacttc
      721 gatccgatgg gcggcctcat gacgatcgtc tcggtgacct tgaccgggtt catcgtcggc
      781 tgggccaagc ccacgcccgt caacccggcc aacctccacg accgccgcaa cggcgaggtc
      841 ttcgtcgccc tcgcaggccc cgcgtcgaac ttcctgatgg ccctcctcgg cgctttcgtc
      901 atccggatcc tcctggcggc cggcgtggac ctcagcggcc tcccggggtt cgtcgcctac
      961 aacttcgtcc tgttcaacgt cctgctggcg atcttcaact tcatcccggt cccgccgctc
     1021 gacggctcgg cgctgctgta ccggttcctc tcgccgcgcc aggcctggca ggtccgtccg
     1081 ttcctgaccc agtacggcat cttcatcgtg ctcggggtcg tcctgctgct cagccgccag
     1141 ctgggcggcc tcatctacgg gatcaccgat gtcctggtgg gcgcgtaagg cccgccagtt
     1201 ccggttgcac ctcgtcgccc gcgtgagccc cgccgagcga gccgcgctga cggcgtggct
     1261 gcacccgccg gagctggacc tgttcgatgc gatgcacgtc gccgaccgcc gccacgggct
     1321 cgacgtggtg gccgcgctgc gaagatccgg ggtgaccgat cgcgacgtgc tcgcggccgg
     1381 gctcctgcac gactgcgcga agggcgacac cggggtcggc ccgcgggtgg cctggtccct
     1441 gggcgaggcg tacgggccgc gggtgctcgc gctcgcggcg cgggtgccgg gctggggcgt
     1501 ggccctcgat cgcctgcgcc accacgcgga ggcgtcggcg tccatggtgg aggcggccgg
     1561 cctgccgccg ttcgccgtcg acctcgtgcg ctaccagtcc gagccccggg accccgagtt
     1621 cggccgcgtg ttccagctcg cggacgaggc cgcctgatgg cgctgtcggg gatgccctcg
     1681 gtcgcgttcg gcgacggcgg ccgtccggag gcggccaccc gggtgaccct gcccgcgttc
     1741 gacgggccgc tcgcgctgct gctctcgctc atcgaggccc gccagctcga cgtgctcacg
     1801 gtgcccctcg gcgcgctcgc ggacgcctat ctcgacgccc tcgcgacgct cgacgaggac
     1861 cgcatgggcc acgtctcggc gttcgtcgcg gtcgcgagcc agctgatcct gatcaagtcg
     1921 cgggcgctgc tgccgcgccg ggccgttgac gggccggtcg cgctggccga cgagggcccc
     1981 gaccccgagg ccgagctgcg tgcccgcctg atcctgtacc gcgcctaccg ggacgccggc
     2041 acgtcgctcc tggacgcggc gacggcacga ggcgccctgt tccatcgcga gtccgtgacg
     2101 gcccgtgccg cggggctggc gggcgcccgg gcgccggccg ccgagccgct gtcgccggcg
     2161 atcctggtcc tcgcgctcga cggcctcgcc aggatcgcac cgcccccggc tccgccgccc
     2221 gagacgatcc gacggacggt cacgctcacc tcgcgggcgg cgatcatccg cgaggccctc
     2281 cgcgaggcac cggcggtcgt cctccaggac ctgctcaagg gcgtgcgcga ccgggtcgtg
     2341 gtcgcggtca cgttcctcgc cctgctcgag ctgatgaagc ggcgcgagat cgtcgtggag
     2401 caggcgcagc cgtggggacc gatcgtggcg cgccggacga ccgcggcgga acgggctgcg
     2461 tcggggtcac aggccatcgt cgacgaggac acgccgctcg acgagtcgct ggagtcgttc
     2521 gcgtgaccga gctcgaggcg gtcgggccgg gcgagctcgc cgccggtgag gccgacgatc
     2581 ccacgaccgc gggcgagctc accgaggcgc agctcgaggc gctgctattc gtggccgaga
     2641 agccgctctc gaggcgtgag atctcgctgc tggccggggt ggaccgggag acggtcgacg
     2701 ccctgctggg cgacctggag gtctcgctcg acgggcgcgg gatccggctg gtcctgtccg
     2761 gcgaccacgt cgagctgacg accgcgcccg acgccggggc cctgatcgcc cgctacgtcg
     2821 gcgcggacgc ggtccgcctg agccc
//
LOCUS       DBNHXO010000039         1675 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1001167, whole genome shotgun sequence.
ACCESSION   DBNHXO010000039 DBNHXO010000000
VERSION     DBNHXO010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1001167"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="AC162A_00830"
     CDS             <1..592
                     /locus_tag="AC162A_00830"
                     /inference="COORDINATES: protein motif:HMM:NF037737.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009410.1"
                     /translation="AEQLGEPLLRRANPVVATYARQEAVDIRISARAEAGRSAVELAD
                     DAEAEVRATLGTYVWASGDTTWSGALAEALAVRGWTLATVESGTDGALVGLLRGLGAV
                     RRSEVASVPGTRADKEPERDLAEADRIRAAAETDVGLAIRASAAGDDTAVNVAVVTPE
                     RRHAERRLAFLRGSAGADRAAIAGAAVLLAVLRNET"
     gene            complement(589..1302)
                     /locus_tag="AC162A_00835"
     CDS             complement(589..1302)
                     /locus_tag="AC162A_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR5009411.1"
                     /translation="MSQARQATEAAAFRAALGGIPLFHDLSDDDLDALTASVRTRRFR
                     RGEVIFHQGDPGDALHVVLSGRIKISSPSDTGVEAILATLRPSEFFGALALLDGAPRS
                     ATATAVSATETLVLPREQFRQLVDDVPSIRDHVLAELAGEIRRLTNHVEELHFLDIAG
                     RLAARLARMADEQGRPTEGEIRLEGPITQGELAAMVGSTRQSVNKLLGWFVDDGLIRM
                     DRDAIVIVDLAGLRMAARR"
     gene            complement(1337..>1675)
                     /locus_tag="AC162A_00840"
     CDS             complement(1337..>1675)
                     /locus_tag="AC162A_00840"
                     /inference="COORDINATES: protein motif:HMM:NF022077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR5009412.1"
                     /translation="VTKALAMLQRLNVPIIGIVENMSAFICPHCGEATEIFGRGGGER
                     FAQQHGIDFLGKIPLDVTVRQGGDVGVPAVAQREPGTAAAALEAVAGQVAARMSIRAD
                     SVQTPVLSIT"
BASE COUNT          227 a          599 c          626 g          223 t
ORIGIN      
        1 cgcggagcag ctcggcgagc cgctcctgcg acgggcgaac ccggtggttg cgacctacgc
       61 ccgccaggag gccgtggaca tccggatctc ggcgcgtgcc gaggccgggc gttcggcggt
      121 ggagctcgcc gacgacgccg aggccgaggt gcgggcaacc ctcgggacgt acgtctgggc
      181 gagcggcgac acgacgtggt cgggcgcact tgcggaggcc ctcgcggtcc gtggctggac
      241 gctggcgacg gtcgagagcg gcaccgacgg cgccctggtc ggcctgcttc gcggtctggg
      301 ggcggtgcgc cgctccgagg tcgcgtcggt cccgggaacc agggcggaca aggagccgga
      361 acgcgacctg gcggaggccg atcggatccg cgccgccgcg gagacggacg tcggcctcgc
      421 gatccgcgcc tcggccgccg gcgacgacac ggcggtcaac gtcgcggtcg tgacgccgga
      481 gcgccggcat gccgagcgtc gtctggcgtt cctgcgcggc agcgcgggcg cggaccgagc
      541 cgcgatcgcg ggggcggccg tcctcctcgc ggtcctgcgc aacgagacct agcggcgggc
      601 cgccatacgc aggccggcga ggtcgacgat cacgatcgcg tcgcgatcca tccggatcag
      661 cccgtcgtcg acgaaccagc cgagcagctt gttcacgctc tggcgggtgc tgccgaccat
      721 cgccgctagc tcgccctgcg tgatcggccc ttcgagccgg atctcgccct ccgtcggcct
      781 gccctgttcg tcggccatcc tcgcgagccg cgcggccaga cgcccggcga tgtcgaggaa
      841 gtgcagctcc tcgacgtggt tggtgaggcg ccggatctcg ccggccagct cggccaggac
      901 gtggtcgcgg atcgacggta cgtcatcgac aagctggcgg aactgctcgc gcgggaggac
      961 cagcgtctcg gtcgcgctca cagcggtcgc ggttgccgag cgcggtgcac cgtcgagcag
     1021 cgccagggcg ccgaagaact cgctcgggcg gagcgtggcc aggatggcct cgacgcccgt
     1081 gtcggacggg ctcgagatct tgatccgacc gctgagcacg acgtgcagcg cgtcacccgg
     1141 atcgccctgg tggaagatca cctccccccg ccggaagcgc cgggtgcgga cactcgcggt
     1201 gagcgcatcg aggtcgtcgt cggagagatc gtggaacagg gggatcccgc cgagcgcagc
     1261 tcggaacgcg gccgcctccg tcgcctggcg cgcctgactc accgccgcat cctacgaccg
     1321 gccggggcgg cgtggctcag gtgatcgaga ggaccggcgt ctggacggag tccgcgcgga
     1381 tgctcatccg ggccgcgacc tggcccgcga cggcctcgag cgccgcggcc gccgtccccg
     1441 gttcgcgctg ggcaaccgca ggcacgccga catccccgcc ctggcgaacc gtgacatcga
     1501 gcgggatctt gcccaggaag tcgatcccgt gctgctgggc aaaccgctcg ccgccgcccc
     1561 gcccgaagat ctcggtcgcc tcgccgcaat gcgggcagat gaaggcgctc atgttctcga
     1621 cgatcccgat gatcggcacg ttcaggcgct ggagcatcgc gagcgccttg gtcac
//
LOCUS       DBNHXO010000040         9253 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4970441, whole genome shotgun sequence.
ACCESSION   DBNHXO010000040 DBNHXO010000000
VERSION     DBNHXO010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9253)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9253
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4970441"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="AC162A_00845"
     CDS             <1..983
                     /locus_tag="AC162A_00845"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF016357.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HIR5009413.1"
                     /translation="SGVVGRLRAIAFAARAGYHLAGARAVVVDDYFFPLYVIRPRPGT
                     VRLQVWHAAGAFKTFGYSVLDRSFGADEAFVEKVAIHCNYSLALVSSMSVAPHYAEAF
                     GQPVDLFTSRFGIPRTDLFADDRRRERARARLRTTYGLPDGRRVVLYAPTFRGETIGN
                     ARYDDLLDLRVMHDALGDGHVVVLKLHPFVRQALVVPTDLGGFAIDASADPDLNELML
                     ISDVLVTDYSSAIYEFSLLGRPIAFLAPDGDAYQRERGFYLDFPADLPGPVFTTTADL
                     AAAIRANAFDLERVRAFAAASFDVVDGRATARIVDEVIVPALRGEAPPNA"
     gene            complement(980..1663)
                     /locus_tag="AC162A_00850"
     CDS             complement(980..1663)
                     /locus_tag="AC162A_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009414.1"
                     /translation="MEWLGVERRRLGTALLVFGLIGMVLAAVIAGGLIAGGIAARNLD
                     DRIAADQAKLSSSLHRIDASIAKLATSTDHAAATLNTTGALIGEAGVVIQDLAGVSDE
                     LANNLNFNILGQQPLAGASSKFRDFSARLRAFQGMTGGLEDNLTTNANDLTGLTAEIE
                     QIGVMTQDLAERVDDFDRAGEIIALLVGGIILGGILVAWLALAAAFCAWAGWRLRKIA
                     AAEAAATQT"
     gene            1677..2609
                     /locus_tag="AC162A_00855"
     CDS             1677..2609
                     /locus_tag="AC162A_00855"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006909275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5009415.1"
                     /translation="MPRPPDGYPEAQEQEGSVERIGFVGLGTMGAAMAANLRRAGFEV
                     TVWNRTPGRADGLVQLGAREAATPAAVAVASDVVVSCVSDTPDVEAVLFGSDGVADGI
                     ARGGLVIDCSTISPTATVDFARRLADAGVGFVDAPVSGGSEGARLATLTIFVGGEPDA
                     VERARPILAAMGKTITRFGPAGSGQAVKAINQVVLAGTYLGVAEGMVLALKAGLDPVA
                     VADALHGGAARSWVLENRSGRMIDNEYPLGFRTALHLKDLVIALEMARGLGASLPVAG
                     IAAQLEAGLVARGHGDEDMSNLARAIRSLSGLDG"
     gene            complement(2631..3821)
                     /locus_tag="AC162A_00860"
     CDS             complement(2631..3821)
                     /locus_tag="AC162A_00860"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR5009416.1"
                     /translation="MAGPRRIGILTGGGDVPGLNAVIKSVVYRATELGYEAIGIRRGW
                     EGLTHQRRGPEPDPEYVRILDRNNTRTIDRTGGTMLHSSRTNPRKMRASSLPAWLDPA
                     TVADRETSDGVYDLTQHVLDTIEHLGLSHLVTIGGDDTLSFSSVLHTAGMPLIAIPKT
                     MDNDVNGTEYCIGFSSAITRAKELINRQRTTLGSHERIGVFRIFGRDAGFTALYTAYV
                     TSTRCLIPEFPYDLDALAELLKEDHRNNPSRYAMVVTAEGAIWHGFEVPEFGEADAFG
                     HRHKANVGEVLATELKARTGIDAVSSELTYDLRSGEPDMLDSMVASTFANIGMDLIRD
                     GVGGRMVAIRDGKYAHVSLDEASKPPRRVQLDAYTPERFRPKYADRLGDPLLLTPSLG
                     PVLT"
     gene            3921..4373
                     /locus_tag="AC162A_00865"
     CDS             3921..4373
                     /locus_tag="AC162A_00865"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF025793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HIR5009417.1"
                     /translation="MNYELFMGEALAEARLAGSRGAPPVGAIAVMNEAMVARGSHRVT
                     KGNDPTAHAVVVALREAARKLATPHLSDVTIFTTLEPCPMCVGALLECDVAALVYAMP
                     NPIDGAAGTVLQLAQHPGLGRRLQVVSGIRRAEAEELNAQLAAVPSGA"
     gene            4752..4837
                     /locus_tag="AC162A_00870"
     tRNA            4752..4837
                     /locus_tag="AC162A_00870"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4786..4788,aa:Ser,seq:cga)
     gene            4893..4980
                     /locus_tag="AC162A_00875"
     tRNA            4893..4980
                     /locus_tag="AC162A_00875"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4927..4929,aa:Ser,seq:gga)
     gene            complement(4999..5427)
                     /locus_tag="AC162A_00880"
     CDS             complement(4999..5427)
                     /locus_tag="AC162A_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5009418.1"
                     /translation="MEPRINLVTLGVTDLERSLRFYRDGLGWRPSSASVEGDVAFFQV
                     GPLVLALWSRSELAKDAGLFDGGGWGAVALAHNVRSQAEADVAVARAIDAGGRLLKAA
                     GPTDWGGYSGYIADPDGHPWEIAWNPHWPLEADGSIRLPD"
     gene            5480..5743
                     /gene="ffs"
                     /locus_tag="AC162A_00885"
     ncRNA           5480..5743
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC162A_00885"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            5782..7410
                     /gene="dnaX"
                     /locus_tag="AC162A_00890"
     CDS             5782..7410
                     /gene="dnaX"
                     /locus_tag="AC162A_00890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR5009419.1"
                     /translation="MTEQIPHQALYRRWRAQTFSEIVGQEAVVATLRNAVATGKVSHA
                     ILFTGPRGTGKTSLARILAKALNCTDLQPNADPCDRCPSCVAIREGRALDLVEIDAAS
                     NRGIEAVRDLRERLAYAPTDLRRKVYILDEAHQITKDAWNALLKSLEEPPEFVSFMFA
                     STHPQEFPPAILSRLQRFDVRRLTIPEITGKLERILAADGREVDPEAIAVIARLAAGG
                     MRDAESILDQLLASTVGPVTADAVRDLLGLADAETVDAFVTAMADGDAAGGIALLDGL
                     EDRGRDLRVFLDQVVDALRDRLIAGLASGASDEAALVAAARRLGSIDPTRLGPGGLRL
                     QLELALLDPAPSPARVVQSQPAAVPAPQVRTARPEPAPVARPPTREPARPPTPVMVDT
                     PAAPEPISEVTAPPAAAEAAAAAEPATADATAGDDLERLRRGWSQIIAVVSKSPALKP
                     LIDACRPISVDGNVVTLGFPEEKDFMREIAEKKRAGIEEGIAGYLGHPVGVRCVVSNL
                     DLLPPLPDDADAAHIMAEAQRIFAEDIADVREVT"
     gene            7508..7816
                     /locus_tag="AC162A_00895"
     CDS             7508..7816
                     /locus_tag="AC162A_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR5009420.1"
                     /translation="MGMGNMQKMVMQMQQEAARVQAELEAARIDASAGGGVVTATVSG
                     KGDLVGLVIDPSAVDPEDVEMLQDLIVSAVGEAIRNARELEADKMGALTAGLRIPGLG
                     "
     gene            7827..8444
                     /gene="recR"
                     /locus_tag="AC162A_00900"
     CDS             7827..8444
                     /gene="recR"
                     /locus_tag="AC162A_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR5009421.1"
                     /translation="MTTAIIEPVARLIEAFARLPGVGPKTAQRLTFHLLRAPDAEART
                     LARALIAVRDEVVFCDTCFNISDAPQCPICRDPGREDVRLCVVEEPLDVLAIERTGEF
                     RGRYHVLHGAISPIDGIGPERLKIRELLARVDEAEARGERIEEVIIATNPTLEGEATA
                     MYLGERLDGHVGSVTRIARGIPVGGDLEYADDVTLIRALQGRREL"
     gene            8444..8629
                     /locus_tag="AC162A_00905"
     CDS             8444..8629
                     /locus_tag="AC162A_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009422.1"
                     /translation="MRELLFEILLRLTKALAAAVLGLLVWLVAVGPLGAQGSVELALL
                     CWLSGAAFILVVQESPI"
     gene            8927..>9253
                     /locus_tag="AC162A_00910"
     rRNA            8927..>9253
                     /locus_tag="AC162A_00910"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1342 a         3295 c         3246 g         1370 t
ORIGIN      
        1 gatcgggtgt cgtgggtcgc ctgcgggcca tcgcattcgc cgcccgggcc ggctaccacc
       61 tcgccggtgc acgggccgtc gtcgtcgatg actacttctt cccgctgtac gtgatccggc
      121 cacgccccgg gacggtccgg ctccaggtct ggcatgcggc aggggcgttc aagacattcg
      181 gctacagcgt cctcgaccgc tcgttcggcg ccgacgaggc gttcgtcgag aaggtggcga
      241 tccactgcaa ctacagcctc gccctcgtgt cgtcgatgtc ggtcgccccg cactatgcgg
      301 aggcattcgg acaaccggtg gacctgttca cgtcgcggtt cggcatcccg cgcacggacc
      361 tgttcgcgga cgatcgccgg cgcgagcgcg cccgcgctcg gctgcgcacg acctacggcc
      421 tgcccgacgg acgccgggtc gtgctctacg ccccgacgtt ccggggcgag acgatcggca
      481 acgcccgcta cgacgacctg ctcgacctgc gcgtcatgca cgatgcgctg ggcgacggcc
      541 acgtcgtcgt cctcaagctc caccccttcg ttcgccaggc cctcgtcgtc ccgacggacc
      601 ttggcgggtt cgcgatcgac gcctccgccg acccggacct caacgagctg atgctgatca
      661 gcgatgtcct ggtgaccgac tactcgagcg cgatctacga gttctcgctg ctcgggcggc
      721 cgatcgcgtt cctcgcgccc gatggcgacg cctaccagcg cgagcgcggc ttctaccttg
      781 acttcccggc cgacctgccc ggaccggtct tcacgacgac cgcggacctc gccgcggcga
      841 tccgggcgaa tgccttcgac ctcgagcgcg ttcgcgcgtt cgccgcggcg agcttcgatg
      901 tggtcgacgg ccgagccacg gcacggatcg tcgacgaggt catcgtccct gccctgcgcg
      961 gcgaggcgcc gcctaacgcc taggtctggg tcgcagccgc ctcggcggcg gcgatcttcc
     1021 gcagccgcca gccggcccag gcgcagaacg cggcggccag cgcgagccag gcgaccagga
     1081 tgccgccgag gatgatcccg ccgacgagca gcgcgatgat ctcgcccgcc cggtcgaagt
     1141 cgtcgacgcg ctcggcgagg tcctgggtca tcacgccgat ctgctcgatc tcggccgtga
     1201 ggcccgtcag gtcgttcgcg ttggtggtca ggttgtcctc gaggccgccg gtcatcccct
     1261 ggaacgcgcg cagccgggcg ctgaagtcgc ggaacttgga cgacgctccc gcgagcggct
     1321 gctggccgag gatgttgaag ttgaggttgt tggcgagctc gtccgagacg ccggcgaggt
     1381 cctggatcac gacgcccgcc tcgccgatca gcgcacccgt ggtgttgagc gtcgccgcgg
     1441 cgtgatcggt ggacgtggcg agcttggcga tcgaggcgtc gatccggtgg agggacgagc
     1501 tgagcttcgc ctggtcggcg gcgatccggt cgtcgaggtt gcgggcggcg atcccgcccg
     1561 cgatcagccc gcccgcgatc accgccgcga ggaccatccc gatcaggccg aagaccagca
     1621 gcgcggtccc caggcgacgc cgctccaccc cgagccattc catcgccgca tcgtatctgc
     1681 cgcgaccccc ggacgggtac cctgaagcgc aggagcagga gggcagcgtg gaacggatcg
     1741 ggttcgtcgg gctcggcacg atgggagcgg ccatggccgc gaacctgcgg cgggcggggt
     1801 tcgaggtcac ggtctggaat cggacgccgg gccgcgccga tgggctcgtc cagctcggtg
     1861 cccgcgaggc ggcgacgccg gcggccgtgg ccgtcgcgtc ggacgttgtc gtgagctgcg
     1921 tctcggacac ccccgacgtc gaggccgtgc tgttcgggag cgacggcgtg gcggacggga
     1981 tcgcccgcgg tgggctggtc atcgactgct ccacgatctc cccgaccgcg accgtggact
     2041 tcgcccgccg actcgccgac gccggcgtcg gcttcgtcga tgcgccggtc tcgggcgggt
     2101 cggagggagc ccgcctcgcg accctgacga tcttcgtggg tggcgagccg gacgccgtcg
     2161 agcgggcccg cccgatcctc gcggccatgg gcaagaccat cacccgcttc ggtccggccg
     2221 gcagcgggca ggcggtcaag gcgatcaacc aggtggtcct cgccgggacg tacctcggcg
     2281 tcgccgaggg gatggtgctc gcgctcaagg ccgggctcga ccccgtcgcc gtcgccgacg
     2341 cgctccacgg cggcgcggcg cgcagctggg tcctcgagaa ccgcagcggt cggatgatcg
     2401 acaacgagta cccgctcggg ttccggacgg cgctgcatct caaggacctg gtgattgcgc
     2461 tcgagatggc gcgcgggctc ggggcgtcgc tcccggtcgc ggggatcgcg gcccagctgg
     2521 aggcggggct cgtcgcgcgc ggccacggcg acgaggacat gtccaacctc gcgcgcgcga
     2581 tccggtcgct gtcgggcctg gacggctgac gggcgccgac cgcccgatcg tcaggtcagg
     2641 acgggcccca gggagggcgt cagcagcagc gggtcgccca ggcggtcggc gtacttcggc
     2701 ctgaaccgtt cgggggtata ggcgtcgagc tggacccggc gcggcggctt cgaggcctcg
     2761 tcgagcgaga cgtgtgcgta cttgccgtcg cggatcgcga ccatccggcc gccgacccca
     2821 tcgcggatca ggtccatccc gatgttcgcg aacgtcgacg ccaccatcga gtcgagcatg
     2881 tcgggttcgc ccgaccggag gtcgtaggtc agctccgacg acaccgcgtc gatcccggta
     2941 cgtgccttga gctcggtggc cagcacctcg ccgacgttgg ccttgtggcg atgcccgaac
     3001 gcgtcggcct cgccgaactc ggggacctcg aacccgtgcc agatcgcgcc ctcggcagtc
     3061 acgaccatcg cgtaccggct ggggttgttg cggtggtcct ccttgagcag ctcggccagg
     3121 gcgtcgaggt cgtaggggaa ctcggggatc aggcagcgcg tggacgtcac gtacgccgtg
     3181 tacagcgccg tgaacccggc gtcgcggccg aagatccgga acacgccgat ccgctcgtgg
     3241 ctgcccagcg tcgtccgctg gcggttgatc agctccttgg cccgcgtgat cgcggacgag
     3301 aaaccaatgc agtactcggt tccgttgacg tcgttgtcca tcgtcttggg gatcgcgatc
     3361 agcggcatcc cggccgtgtg caggaccgac gagaacgaga gcgtgtcgtc gccgccgatg
     3421 gtgacgaggt gactgagccc gaggtgctcg atcgtgtcga gcacgtgctg ggtcaggtcg
     3481 tacacgccgt ccgaggtctc gcggtcggcg accgtcgccg ggtcgagcca ggcgggcagg
     3541 gacgaggcgc gcatcttgcg cgggttggtg cgcgagctgt ggagcatcgt gccgccggtc
     3601 cggtcgatgg tccgcgtgtt gttgcggtcg aggatccgga cgtactcggg gtcgggctcc
     3661 ggcccgcgtc gctggtgggt caggccctcc cagccacgcc ggatgccgat cgcttcgtag
     3721 ccgagctcgg tcgcgcggta gacgacgctc ttgatgacgg cgttgaggcc cggcacgtca
     3781 ccgccgccgg tgaggatgcc gatgcgtcga ggtccagcca attggggctc cgcagcaagg
     3841 gcccggccga cggccgaacc gccgaacatc ctagcgccgg ggctgggttg cgcgttggtg
     3901 acggtcgtat gatcgcgccc atgaactacg agctgttcat gggcgaggcc ctcgcggagg
     3961 ctcgtctggc cggctcacgc ggcgcgccgc cggtcggggc catcgcggtg atgaacgagg
     4021 cgatggtcgc ccgcgggtcg caccgggtga ccaagggcaa cgacccgacg gcccacgccg
     4081 tggtcgtcgc actgcgcgag gctgcgcgca agctcgcgac accgcatctc tccgatgtga
     4141 cgatcttcac cacgctcgag ccctgtccga tgtgcgtggg ggcgctcctc gagtgcgacg
     4201 tcgccgcgct cgtgtacgcg atgcccaacc cgatcgatgg tgccgccggg accgtcctcc
     4261 agctcgcaca gcaccccggc cttggccgcc ggctgcaggt cgtgtccggg atccgccgcg
     4321 ccgaggccga ggagctgaac gcgcagctcg ccgcggtgcc gtcgggcgcc tgaccgctgg
     4381 ccgcgtccac cgcgattcgc cccgccggcc atccgccacg cattcatgcg gcgaatgcaa
     4441 gctgaggcga gccctgccag gcagtcacgg ggtgaatgcg aacggtcaga atccagcgtc
     4501 cagacgacca tccatgcacg gactgaatgc tgggccaagt gcacggcccg aaggtcagcc
     4561 ctgcattcac gccatgcatg caaggcagga tcgtctcgcc tcctgccgca cggccgccga
     4621 tccggctcga cgctgagcca ggcagagccg tcgtcagcat tcactcgatg catggatggc
     4681 acggtcggac ggcgccgggc cccccgccgc atggccgagg ctcggcaggg ccctttgtta
     4741 tcctctcccg cggagaggtg tccgagtggt tgatggtgcc gctctcgaaa agcggtgtgc
     4801 gaaagcaccg tgggttcgaa tcccaccctc tccgccacgc gcgcaaggcc acgacccggc
     4861 ggcttccccc gccgggtcgt atcgtgtctc ccggagaggt cgcctagtgg cctagggcgc
     4921 cgcactggaa atgcggtatg gggcaaccca tcgcgggttc gaatcccgcc ctctccgcca
     4981 tagccgcggt tcagcccgct agtcgggcag ccggatcgag ccgtcggctt cgagcggcca
     5041 gtgcgggttc caggcgatct cccacgggtg cccgtcggga tccgcgatat acccggagta
     5101 gccgccccag tcggtcggcc cggcagcctt gagcagccgc ccgccggcgt cgatcgcccg
     5161 ggccaccgcc acgtcggcct ccgcctgcga gcgcacgttg tgggcgagcg ccaccgcgcc
     5221 ccaaccgccg ccgtcgaaca gcccggcatc cttcgccagc tccgaccgcg accacagcgc
     5281 gaggacgagc ggccccacct ggaagaacgc aacgtcgccc tcgaccgacg ccgacgatgg
     5341 gcgccagccg aggccgtcgc ggtagaagcg cagggagcgc tcgaggtcgg tcacgcccag
     5401 cgtgacgagg ttgatccggg gctccatcgg gcacctcaag ggacgggccg gacaagcggc
     5461 ccctgctatc atccgcctgc cgtgctaggc ggggaactag cggtgccctg tacctgcaat
     5521 ccgctccagc agggccgaat tcccccccga ggtcggcgtt ctcgagagcc gacgacgttc
     5581 gcgacgttga agaccgggtc ccgcgcagcg gcgactcgtg aaccgtgtca ggtccggaag
     5641 gaagcagcac tcagcggtga tcgccgggtg ccgcggaatg gcctggtccg agttgcgggc
     5701 gtcggtgggc gcgggagcgt cctgatcgac ggggggtgca cggcaaaact cccgcctcga
     5761 cctgcccgcc gaggagcgcc gttgaccgaa cagatcccgc accaggcgct ctatcgccgc
     5821 tggcgagccc agacgttctc cgagatcgtc gggcaggagg cggtggtcgc cacgctgcgc
     5881 aacgcggtcg cgaccggcaa ggtctcgcac gcgatcctgt tcacgggtcc gcgcgggacg
     5941 ggcaagacat cgctcgcgcg gatcctcgcc aaggcgctga actgcacgga cctgcagccc
     6001 aacgccgacc cctgtgaccg ctgcccgtcg tgcgtcgcga tccgcgaggg ccgagccctc
     6061 gacctggtcg agatcgacgc cgcgtccaat cgcgggatcg aggccgtccg cgacctccgc
     6121 gagcgcctcg cctatgcccc gacggacctc cggcgcaagg tctacatcct cgacgaggcg
     6181 caccagatca ccaaggacgc ttggaacgcg ctcctgaagt cgctcgagga accccccgag
     6241 ttcgtcagct tcatgttcgc gtccacccat ccccaggagt tcccgccggc catcctgtcg
     6301 cggctccagc ggttcgacgt ccggcgcctg accatccccg agatcaccgg gaagctcgaa
     6361 cgcattctgg ccgccgacgg acgggaggtc gaccccgagg ccatcgcggt catcgcccgg
     6421 ctggcggcgg gcgggatgcg cgacgccgag tcgatcctcg accagctgct cgcatccacg
     6481 gtgggtccgg tgacggccga tgcggtgcgc gacctcctgg gcctcgccga tgccgagacc
     6541 gtcgacgcgt tcgtgaccgc gatggccgac ggcgacgcgg ccggaggtat cgcgctgctc
     6601 gacgggctcg aggatcgagg ccgggacctg cgcgtgttcc tcgaccaggt cgtggacgcg
     6661 ctgcgggacc ggctcattgc cgggctggcg tcaggcgcgt ccgacgaagc cgcgctcgtc
     6721 gccgccgccc gccgcctggg ctcgatcgac ccgacacggc tcggtcccgg gggcctgcgc
     6781 ctccagttgg agctggccct cctcgacccg gcgccgtcgc ccgcgcgcgt cgtccagtcg
     6841 cagcccgccg cggtccctgc cccgcaggtc cggaccgctc gtcccgagcc ggcacccgtc
     6901 gccaggccac ccacccgcga gccggcccgt ccgccgacac cggtcatggt cgacacgcct
     6961 gccgccccgg agccgatctc cgaagtcacc gcaccgcccg cggccgccga ggctgcggca
     7021 gccgccgagc cagcgacggc cgacgccacc gccggcgacg acctcgagcg cctccggcgc
     7081 ggctggtcgc agatcatcgc cgtcgtcagc aagagcccgg ccctgaagcc gctcatcgat
     7141 gcctgccgcc cgatctccgt cgatggcaac gtcgtgacgc tcggcttccc cgaggaaaag
     7201 gacttcatgc gcgagatcgc ggagaagaag cgagccggga tcgaggaggg gatcgccggc
     7261 tacctcggcc accccgtggg cgtccgctgt gtggtgagca acctcgacct gctgccaccc
     7321 ctgcccgacg acgcggacgc cgcgcacatc atggccgagg cgcagcgcat cttcgccgag
     7381 gacatcgccg acgttcgcga ggtcacctga ccgcctctcg atggtgcgcg aggcccccgc
     7441 atcggcgaga cttgccgcac gaacagcacg tatcggaccc accaacccga ccggaggacc
     7501 cgtacccatg ggcatgggca acatgcagaa gatggtcatg cagatgcagc aggaggcggc
     7561 ccgcgtccag gccgagctcg aggctgcgcg gatcgacgcg tcggccggtg gcggcgtggt
     7621 cacggccacc gtcagcggca agggcgacct cgtcggtctc gtcatcgacc cgtccgcggt
     7681 ggaccccgag gacgtcgaga tgctccagga cctgatcgtc tcggccgtcg gcgaagcgat
     7741 ccgcaacgcc cgtgaactcg aagcggacaa gatgggcgcg ctgaccgcgg gcctgcgcat
     7801 ccccggcctc ggctgatcgc gcgcgcgtga ccaccgccat catcgagccc gtcgcgcgcc
     7861 tcatcgaggc cttcgcccgg ctgcccggcg tcggacccaa gacggcgcag cggctcacct
     7921 tccacctcct gcgtgccccc gacgccgagg cgcggaccct cgcgcgtgcc ctgatcgcgg
     7981 tccgcgacga ggtcgtgttc tgcgacacct gcttcaacat cagcgacgcg ccgcagtgcc
     8041 cgatctgccg cgacccgggt cgcgaggacg tgcggctgtg cgtggtggag gaaccgctcg
     8101 acgtgctggc catcgagcgg accggcgagt tccgcggccg ctaccacgtg ctccacgggg
     8161 cgatcagccc gatcgacggc atcgggcccg agcggctcaa gatccgcgag ctcctcgcgc
     8221 gcgtcgatga ggcggaggcg cgcggcgagc ggatcgagga agtgatcatc gcgaccaacc
     8281 cgaccctgga gggcgaagcg accgccatgt acctgggcga gcggctcgac ggccacgtcg
     8341 ggtccgtgac gcggatcgcc cgcggcatcc cggtcggcgg cgacctcgag tacgccgacg
     8401 acgtcacgct gatccgggcg ctccagggcc gccgggagct ctgatgcggg agctgctgtt
     8461 cgagatcctc ctgcggctga cgaaggcgct cgccgcggcg gtgctgggcc tcctcgtctg
     8521 gctggtggcc gtggggccgc tgggggcaca gggctcggtc gagctggcgc tcctgtgctg
     8581 gctcagcggc gcggccttca tcctcgtggt ccaggagagc ccgatctagg ctcgccacag
     8641 ggcgtgccgc ggacgccggc gcacgtttga cgacccgccg cgacgcgcca tatcatccgc
     8701 cctcgtgcct cacgacccga ggctcgccgg tcccctgcgc aagcagggcc aatccccgat
     8761 gccccgtttg acacgggggg cggtctgcgc aatcatgagc agtccgcgct ctgggcggta
     8821 aaggccgggc gctgcacctt aacaactgaa gagtggtagg aacagtcagc acaagaacgt
     8881 gcctggacca agctcttgag attcaacttg atcgcaaggt caagttttcg cggagagttt
     8941 gatcctggct caggataaac gctggcggcg tgcatgagac atgcaagtcg aacgaacgag
     9001 cttgctcgtt agtggcggac ggctgagtaa cgcgtgggtg acctgccctg aagtggggga
     9061 taactgcccg aaagggtggc taataccgca tgtggcgaca cgttcattcg tgtcatcaaa
     9121 gccgcgaggc gcttcgggag gggcctgcgt ccgattagct cgttggtggg gtaatggctc
     9181 accaaggcga cgatcggtag gtggtctgag aggacgatca cccagactgg gactgagaca
     9241 cggcccagac tcc
//
LOCUS       DBNHXO010000041         6104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2588460, whole genome shotgun sequence.
ACCESSION   DBNHXO010000041 DBNHXO010000000
VERSION     DBNHXO010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2588460"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2154
                     /locus_tag="AC162A_00915"
     CDS             <1..2154
                     /locus_tag="AC162A_00915"
                     /inference="COORDINATES: protein motif:HMM:NF037144.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HIR5009423.1"
                     /translation="GSLGSISPIDCVTEAPACTATVTYTPDDDVNGSDEFEFTATDED
                     VESSSPVTVSITIDPVNDPPNFTRGPDVTDDEDGGGRTIVGWATSIDEGAPNESSQTV
                     TFDVTNDDNGLFTGGGQPEIASNGTLTYTLAANASGSTTVSVQAKDNGGTANGGDDSS
                     AIKTLTITVGDVNDAPHFTAGPDEHVDEDPGTQTVTDWATGISTGPANESGQVITFDI
                     TANTNEELFASGPSVDSDGTLTYRPADDAHGTATIDIRVVDNGGTANGGDDSSPNQSF
                     TVTVDSVNDAPSFTKTTVPGEADPTSAEDAPLQTVFGWAQNVSEGAANESGQDVTFVI
                     TGNDNTALFSTQPSVSSSGDLSFKPAANKNGTANLTIHLEDDGGTANGGVDTSADQTF
                     SITVSGVNDAPSANNDAFTVRLGGATAIDIKANDNGGPGDTGDPLTITSVTGAAKGYI
                     TIGGTGGATTLSYDPMGCATGTDTFTYTLTDGGGLTDTATVFVTINGPASFPFADGPR
                     PAFVSGSTIGTTTPVRMSWCGLTSGTSLKSYRIYQAKNGGSFKTLIKSTTGTSSTRNL
                     DVAPDASQFRARITDRKNHDSTGTGPKFRVVRTQDTSGSIVYSSGWSKSTKGNPSGSS
                     THATSGANKTATFTFTGRAFAIVGTQAKKHGSFNVYLDGVKVTTSAISTKSKKTHERR
                     VLYARSTTAGSHTIEIRTTGSGRVDLDAILSIAQP"
     gene            2221..2436
                     /locus_tag="AC162A_00920"
     CDS             2221..2436
                     /locus_tag="AC162A_00920"
                     /inference="COORDINATES: protein motif:HMM:NF021073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HIR5009424.1"
                     /translation="MNTGFGAYFALFSEIGMILLVTTLAGVAAGYWADQQLGTLPIFV
                     LVGFLAGAGIGTVGIYRLLTRFLKRFD"
     gene            2500..3483
                     /gene="atpB"
                     /locus_tag="AC162A_00925"
     CDS             2500..3483
                     /gene="atpB"
                     /locus_tag="AC162A_00925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR5009425.1"
                     /translation="MTGDTAQKKRGISNPVAIGLLLIFVIVIDVFAFLLFPPFDKEHP
                     ESLTCEFPVCYINGNLEFPAPHPVWSLDGQPQSTDLITFQVSLSSTLVTMFFITIVVI
                     VLLWALSRKHELKPGRGQNAIEYVYEMLESFGTSLGGPRSKPYIPLFAGFFLFILFCN
                     WSGLIPPIGKVDQLRAPTSDLNVTIGLALVAFVFIEYQGFKHLGAGYLGKFFPFGEFK
                     KGAGAGAIALFVGLTELLLEFVKPITLSMRLFGNIYGGEVALGVLTALTLAIIPMAML
                     ALEVLLNAVQALIFATLTLMFTLVAIEGHGQEHEAAESAEATIHAVEAAAH"
     gene            3546..3767
                     /locus_tag="AC162A_00930"
     CDS             3546..3767
                     /locus_tag="AC162A_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009426.1"
                     /translation="MENLGALAALGVIGPGIGIGILAGLAAAAIGRNPDAAGEIRGLA
                     IILAAFAEGLGVLAVVVGLLAIFIKPAAG"
     gene            3789..4361
                     /gene="atpF"
                     /locus_tag="AC162A_00935"
     CDS             3789..4361
                     /gene="atpF"
                     /locus_tag="AC162A_00935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR5009427.1"
                     /translation="MDTFALAGEVLRQVAIPAAEGAEPAKFQINLFWIIVAAANFVVF
                     FLIVQRIAFGGLVRTLNDRRSRIEQGLQDAEQARRDRETAEQERQSTLQEARREANDI
                     LNRAQKVAAEAREQDIAATRDEIARLRERATVEIDAERQRAVAELRGEVTDLALAAAG
                     RVVGESMTGDRQRKLVADFLADPAAGGSAG"
     gene            4375..4926
                     /gene="atpH"
                     /locus_tag="AC162A_00940"
     CDS             4375..4926
                     /gene="atpH"
                     /locus_tag="AC162A_00940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR5009428.1"
                     /translation="MARPATAGRRYAEAAFQVALRDGELDGWRDDLAHAADILGRPEV
                     QAVIDNPAIPSAQRHAVVGKLLEGRVRPGALRLVSLLVERGRAREIQRVSDQYVRQLN
                     AHRGVIVATVTSAAPLSNDETAEIRTRVEAMAGSSIDLRTAVDPELLGGLTVQIGDRL
                     LDASIRGRLERLRDQLNAGTRPR"
     gene            4948..>6104
                     /gene="atpA"
                     /locus_tag="AC162A_00945"
     CDS             4948..>6104
                     /gene="atpA"
                     /locus_tag="AC162A_00945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR5009429.1"
                     /translation="MAIRSDEIISIIKSQIESFDATSETRSVGTVVEVGDGIAQIYGL
                     DAALASEMLEFPGGVMGMALNLEEETVGAVILGDPSAIKEGDTVRTTGRVVEVPVGEV
                     LLGRVVDPLGNALDGKGEIPRTVTRPVERIAPGVITRQSVDTPVQTGIKAIDALIPIG
                     RGQRELIIGDRQTGKTAIAIDTIINQKGSDLVCIYVAIGQKLSTVAQTVATLEKYGAM
                     EHTIVVVAGAEDPAPLQYLAPYAGVAMGEEIMENGVQVGDRLVKDALCVYDDLSKHAW
                     AYREMSLLLRRPPGREAYPGDVFYLHSRLLERAARMSEANGGGSLTALPIVETQANDV
                     SAYIPTNVISITDGQIFLETDLFNAGQRPAMNIGISVSRVGSAAQTKAMKKV"
BASE COUNT         1063 a         2183 c         2010 g          848 t
ORIGIN      
        1 ggcagcctcg gctcgatctc accgatcgat tgcgtcaccg aggcacccgc ctgcacggcg
       61 accgtgacct ataccccgga tgacgacgtc aacggcagcg acgagttcga gttcacggcg
      121 acggacgagg acgtcgaatc gtccagcccc gtgaccgtgt cgatcacgat cgatcccgtg
      181 aacgatccgc ccaacttcac ccgcggcccg gacgtcaccg acgacgagga tggtggcgga
      241 cggacaatcg tcggctgggc gacctcgatc gacgagggag cccccaacga gtcctcacag
      301 acggtgacct tcgacgtcac caacgacgac aacggcctgt tcaccggcgg gggccagccc
      361 gagatcgcgt ccaacggcac gctgacctac acgctcgcgg cgaacgcgtc cgggtccacg
      421 accgtctcgg tccaggccaa ggacaacggc ggcacggcca acggcggcga cgacagcagc
      481 gccatcaaga cgctcacgat cacggtcggc gatgtgaacg acgccccgca cttcactgcg
      541 ggtcccgacg agcatgtcga cgaggatccg ggcacccaga ccgtcaccga ttgggcgacc
      601 ggcatctcca ccggcccggc gaacgaaagc gggcaggtga tcacgttcga catcaccgcg
      661 aataccaacg aagagctgtt cgcgagcggg ccctcagtcg acagcgacgg aacgctgacc
      721 tatcggccgg ccgatgacgc ccacgggacc gcaacgatcg acatccgggt cgttgacaac
      781 ggcggcaccg cgaacggcgg cgacgacagc agccccaacc agtcgtttac cgtgaccgtg
      841 gacagcgtca acgacgcgcc ctcgttcacc aagacgaccg tcccggggga ggccgacccg
      901 acgtccgccg aggacgcccc gctccagacc gtcttcggct gggctcagaa cgtctctgag
      961 ggtgcggcca acgagagcgg tcaggacgtc acgttcgtca tcacgggcaa cgacaacacg
     1021 gccctgttct cgacccagcc gtcggtgagt tcgtccggcg acctgtcgtt caagcccgcg
     1081 gcgaacaaga acgggacggc gaacctgaca atccacctcg aggacgacgg cggcaccgcc
     1141 aacgggggcg tcgacacgag cgccgaccag acgttctcga tcaccgtcag cggtgtcaac
     1201 gacgccccga gcgcgaacaa cgacgcgttc accgtgcgcc tcggcggcgc gacggccatc
     1261 gacatcaagg ccaacgacaa cggcggcccg ggcgacaccg gcgacccgct cacgatcacc
     1321 tccgtcacgg gcgccgcgaa gggctacatc acgatcggcg gcaccggcgg agcgacgacc
     1381 ctctcgtacg atccgatggg ctgcgcgacg ggcaccgaca ccttcaccta cacgctcacc
     1441 gatggcggcg gcctgacgga cacggcgact gtgttcgtca ccatcaacgg cccggccagc
     1501 ttcccgttcg ccgacggccc gaggccggcg ttcgtctcgg gcagcacgat cggcaccacg
     1561 acgccggtca ggatgtcctg gtgcggcctc acgagcggga cctcgctcaa gtcgtaccgc
     1621 atctaccagg ccaagaacgg cggctcgttc aagacgctga tcaagtcgac gacggggacc
     1681 tcgagcaccc gcaatctcga cgtcgctccg gacgcctccc agttccgggc caggatcacc
     1741 gaccggaaga accacgactc gaccgggacc ggccccaagt tccgcgtcgt ccggacccag
     1801 gacaccagtg gctcgatcgt ctactcgtcg ggctggtcga agtcgaccaa gggcaatccc
     1861 tcgggcagca gcacccacgc gacgagcggc gcgaacaaga cggcgacgtt cacgttcacc
     1921 ggccgcgcat tcgcgatcgt cggcacccag gcgaagaagc acggcagctt caacgtctac
     1981 ctcgacggcg tgaaggtgac gacgtcggcg atcagcacga agtcgaagaa gacccacgag
     2041 cgcagggtgc tctacgcgcg ttcgacgacg gccgggagcc atacgatcga gatccggacc
     2101 accggcagcg gccgcgtgga cctggacgcc atcctctcga tcgcgcagcc ctagcaccgc
     2161 tgccgccgtt ccgaatcggc gggggcctct ggtatagtcc agcgcggtga tcggtcgccc
     2221 atgaacaccg gtttcggagc gtatttcgca ctgttctccg agatcgggat gatcctcctg
     2281 gtgacgaccc tcgccggtgt ggcagcgggc tactgggcag accagcagct cgggaccctg
     2341 ccgatcttcg tcctcgtcgg cttcctcgcc ggcgccggga tcgggacggt cgggatctac
     2401 cgcttgctca cacggttcct gaagcggttc gactaaaccg gccacggaac gggcacggac
     2461 gagcgtggcc catggccgcg cggctgcttg ggaggaaacg tgacgggcga cacggcgcag
     2521 aagaagcgcg gtatcagcaa ccccgttgcc atcgggctgc tgctgatctt cgtgatcgtc
     2581 atcgacgtgt tcgccttcct gctgtttccg cccttcgaca aggaacaccc ggagagcctg
     2641 acctgcgagt tcccggtctg ctacatcaac ggcaacctgg agttcccggc accccacccg
     2701 gtgtggagcc tcgacggcca gccccagagc acggacctga tcacgttcca ggtgagcctg
     2761 tcctcgacgc tggtcacgat gttcttcatc acgatcgtgg tgatcgtgct gctgtgggcg
     2821 ctgagccgga agcacgagct gaagccaggc cgcggtcaga acgcgatcga gtacgtctac
     2881 gagatgctcg agagcttcgg cacctcgctc ggcggcccgc ggtccaagcc gtacatcccg
     2941 ctgttcgcgg gcttcttcct gttcatcctg ttctgcaact ggagcggcct gatcccgccg
     3001 attggcaagg tcgaccagct gcgggcgccg acgagcgatc tcaacgtcac gatcggcctc
     3061 gcgctggtcg cgttcgtgtt catcgagtac cagggcttca agcacctggg cgccggctat
     3121 ctgggcaagt tcttcccatt cggggagttc aagaagggtg cgggcgccgg ggccatcgcg
     3181 ctgttcgtcg ggctcaccga gctcctgctg gagttcgtga agccgatcac gctgtcgatg
     3241 cgactcttcg gcaacatcta cggcggcgag gtggcactgg gcgtgctgac ggccctgacc
     3301 ctcgcgatca tccccatggc catgctcgcg ctcgaagtgc tcctcaacgc cgtccaggcg
     3361 ctgatcttcg ccaccctgac cctcatgttc acgctcgtcg cgatcgaggg tcatggccag
     3421 gagcacgagg cggcggagtc cgccgaagcc acgatccacg ccgtcgaagc ggcggcgcac
     3481 tgaccacccg accacgggac ccaagacccg ggactgaggc ccggaccacg gaggcaacac
     3541 ggaacatgga aaacctcggc gcccttgcgg ccctcggtgt catcggaccc ggcatcggca
     3601 tcgggatcct cgccggcctg gccgccgccg cgatcgggcg caaccccgac gcggccggtg
     3661 agatccgcgg cctcgcgatc atcctcgcgg cgttcgcaga gggcctcggc gtcctcgcgg
     3721 tcgtcgtcgg cctgctcgcg atcttcatca agcccgccgc gggctgacct gcggtagcgg
     3781 tgacgcccat ggataccttc gccctcgccg gggaggtcct gcgccaggtc gccataccgg
     3841 cggcggaagg agccgagccc gcgaagttcc agatcaacct gttctggatc atcgtggccg
     3901 cggccaactt cgtcgtgttc ttcctgatcg tccagcgcat cgcgttcggc ggcctcgtca
     3961 ggaccctcaa cgaccggcgc tcccggatcg agcaggggct gcaggacgcg gagcaggcgc
     4021 gacgggaccg ggagacggcg gagcaggagc ggcagtccac cctccaggag gcccggcgcg
     4081 aggccaacga catcctcaac cgcgcccaga aggtcgcggc cgaggcccgt gaacaggaca
     4141 tcgccgcgac ccgcgacgag atcgcccggc tgcgcgagcg cgcgacggtc gagatcgacg
     4201 cggagcgcca gcgcgccgtc gccgagctcc ggggagaggt caccgacctc gcccttgcgg
     4261 ccgccggccg cgtggtgggc gagtcgatga caggcgaccg ccagcgcaag ctcgtggccg
     4321 acttcctcgc cgatccggcc gccggcgggt ccgccggctg atggtgcgcg actgatggcc
     4381 cgcccggcca ccgccggccg ccggtacgcg gaggcagcct tccaggtcgc cctgcgcgac
     4441 ggcgagctcg acggctggcg cgacgacctc gcgcacgcgg ccgacatcct cggtcgcccc
     4501 gaggtccagg ccgtcattga caacccggcc atcccgtcgg cccagcggca cgcggtggta
     4561 gggaagctgc tggagggccg ggtccgcccg ggcgccctcc ggctcgtgag cctgctcgtc
     4621 gaacgcgggc gggcgcgcga gatccagcgg gtgagcgacc agtacgtccg ccagctgaac
     4681 gcgcatcgtg gggtgatcgt ggccaccgtc acgagcgccg cgcccctctc gaatgacgag
     4741 accgccgaga tccgcacgcg ggtcgaggcg atggccggtt cctcgatcga cctccgcacg
     4801 gcggtcgatc ccgagctgct gggcgggctg accgtccaga tcggcgaccg gctcctcgac
     4861 gcctcaatcc gcggccgcct cgagcggctg cgcgaccagc tcaacgcggg cacccggccc
     4921 cgctagcagc gcccgaacgg agacgccatg gccatccggt cagacgagat catcagcatc
     4981 atcaagagcc agatcgagtc gttcgacgcg accagcgaga cccgcagcgt gggcaccgtg
     5041 gtcgaggtcg gcgacgggat cgcgcagatc tacggcctcg acgcggcgct cgcgtccgag
     5101 atgctcgagt tccccggcgg ggtgatgggc atggccctga acctcgagga ggagacggtc
     5161 ggcgcggtca tcctgggcga cccatcggcg atcaaggagg gcgacaccgt ccggacgacc
     5221 ggccgcgtgg tcgaggtccc ggtcggcgaa gtgctcctgg gccgggtcgt ggaccccctg
     5281 ggcaacgcgc tcgacggcaa gggcgagatc ccgcgcaccg tgacccgtcc ggtcgagcgc
     5341 atcgcgcccg gcgtcatcac ccgccagtcg gtggacacgc cggtccagac gggcatcaag
     5401 gccatcgacg ccctgatccc gatcggccgc ggccagcgcg agctgatcat cggtgaccgc
     5461 cagaccggca agaccgccat cgcgatcgac acgatcatca accagaaggg cagcgacctc
     5521 gtctgcatct acgtcgcgat cgggcagaag ctctcgaccg tggcgcagac cgtcgcgacg
     5581 ctcgagaagt acggcgcgat ggagcacacg atcgtcgtcg tcgccggcgc cgaggatccc
     5641 gccccgctcc agtacctcgc cccgtacgcg ggcgtcgcga tgggcgagga gatcatggag
     5701 aacggcgtcc aggtgggcga ccgcctggtc aaggacgcgc tgtgcgtcta cgacgacctg
     5761 tccaagcacg cctgggcgta ccgggagatg tcgctgctgc tgcgccgccc ccccggccgc
     5821 gaggcctacc cgggcgacgt gttctacctc cacagccggc tgctcgagcg agcggctcgc
     5881 atgagcgagg cgaacggcgg cggctcgctg accgccctgc cgatcgtcga gacgcaggcc
     5941 aacgacgtgt ccgcgtacat cccgaccaac gtgatctcga tcacggacgg ccagatcttc
     6001 ctcgagaccg acctgttcaa cgccggccag cggcccgcga tgaacatcgg catctcggtc
     6061 tcgcgcgtcg gctccgccgc gcagaccaag gcgatgaaga aggt
//
LOCUS       DBNHXO010000042         2318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_208792, whole genome shotgun sequence.
ACCESSION   DBNHXO010000042 DBNHXO010000000
VERSION     DBNHXO010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_208792"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1076)
                     /locus_tag="AC162A_00950"
     CDS             complement(135..1076)
                     /locus_tag="AC162A_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5009430.1"
                     /translation="MSGRPIVSAQTRQLAEFGILTTVLIWSANFVIVKAAIGEIGPFS
                     FTAARYLVASLTLLAILWWRQGSIRPPAGYGWALLALGVLGFGCYQVLWTTGLTMISA
                     GDSALILAVSPVLTALLAAAVGMDRLTAPKLAGALLAFAGVVVVIGASHALSLGSSLL
                     GDALTLGAAVVWAVYSVGASKVVRSIDPLRATTWTVIAGAAFLVPLGVAESVASPPVE
                     VTIAIIGAVLYSGVLAAGVANVFVFIAIRYVGPTRTTAMQLLLPAGAVVLGAIFLAEP
                     IGLPQVVGGAVIVAGVWLTRRSSILPASMRARLSSAS"
     gene            complement(1154..1309)
                     /locus_tag="AC162A_00955"
     CDS             complement(1154..1309)
                     /locus_tag="AC162A_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009431.1"
                     /translation="MKALRTVAVALGITLGALIVIPLAVLAGLFVWLKVTEEDDEEAL
                     ELEQEGI"
     gene            complement(1384..2058)
                     /locus_tag="AC162A_00960"
     CDS             complement(1384..2058)
                     /locus_tag="AC162A_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009432.1"
                     /translation="MRIYDGSPRQDFEEVFRSIGAFLDSRGMRDILIVEVPDGFVVQG
                     LVAATTAGGSWSDTMGQVNKETLSFFDDDIAKFMEEAAARRGKGGAQPAMAAAGTFER
                     SLRVIGRWMDAQRPKDIFLFEQGGAYVIRLLETGQAGVHHTLSEFTREDIETLISDGP
                     QLRNKPKPASAGGPIQAVAPMSAGVPPTPAPAPEVAAVPASVPAPEAAAAPEAAASST
                     WGEPAS"
     gene            complement(2106..>2318)
                     /locus_tag="AC162A_00965"
     CDS             complement(2106..>2318)
                     /locus_tag="AC162A_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009433.1"
                     /translation="LAVVPDGPSSIGGPNSVWTSVTGTSAPDGTTIVLALCVTGALAT
                     LAAIATFRSSERRARDLGLLDRTTGS"
BASE COUNT          369 a          816 c          838 g          295 t
ORIGIN      
        1 gtccgagacg tcggtgcgcg cgatcgtgcc gtcgtagtcg acgaggatcg cgagcggcgg
       61 ctggccgggt cgcagcggcg gcaccggcgc cggcgggagg acggacgacc ggggcgggtc
      121 gggcagggct gaggtcacga ggccgaggat agccgcgccc gcatcgacgc cgggaggatc
      181 gacgagcggc gcgtgagcca gactcccgcc acgatcaccg cgccgcccac cacctgcggc
      241 agaccgatcg gctcggccag gaagatcgcc ccgaggacca ccgctcccgc cggcaggagc
      301 agctgcatcg cggtggtccg ggtcgggccg acgtatcgga tggcgatgaa cacgaagacg
      361 ttggcgacgc cagccgccag cacccctgag tagaggacgg cgccgatgat tgcgatcgtc
      421 acctcgacgg gcggactcgc gaccgactcc gcgacaccca gcgggaccag gaacgcggcg
      481 cccgcgatga cggtccaggt cgtggcgcgc aacggatcga tcgaccgcac gaccttgctc
      541 gcgccgaccg agtacaccgc ccagaccacg gccgcaccga gcgtgagcgc gtcgccgagc
      601 agcgacgagc cgagcgagag cgcgtgcgac gccccgatca cgacgacgac gcccgcgaac
      661 gcgagcagcg ccccggcgag cttgggtgcc gtcagccggt ccatccccac cgcggcggcg
      721 aggagcgcgg tcaggaccgg cgataccgcc aggatcagcg ccgagtcgcc tgcgctgatc
      781 atggtcagcc cggtcgtcca caggacctgg tagcacccga agccgagcac gccgagcgcc
      841 aggagcgccc acccataccc cgccggcggg cggatcgacc cttgtcgcca ccacaggatc
      901 gccagcagcg tgagtgacgc cacgaggtat cgggcagcgg tgaacgagaa cggcccgatc
      961 tcgccgatcg cggccttgac gatgacgaag tttgccgacc agatcaggac ggtcgtgagg
     1021 atgccgaact cggccagctg acgggtctgg gcggagacga tgggtcgccc ggacaccgaa
     1081 acgcccgcct ggtcggcggg cgtcgcgtgg ctcggcgggc cggcggacgg gtccgccggc
     1141 ggaggcgagt cgctcagatg ccttcctgct cgagctcgag ggcctcttcg tcgtcctctt
     1201 cggtgacctt gagccagacg aacaggccgg ccagcacggc cagcgggatg acgatcaggg
     1261 cgccaagggt gatccccagg gcgactgcaa cggtccgcaa cgccttcatc gggttgggcc
     1321 tcctacgaga gtgacggtca ggacgtgatt gtgccccccg cggcgccgcg tgtcacccgg
     1381 acgttagctg gcgggctcgc cccaggtcga cgatgcggct gcctccgggg cggcggctgc
     1441 ctccggggcg ggcaccgagg cgggcaccgc tgcgacctcc ggggcgggcg ccggggtggg
     1501 cggcacgccc gcggacatgg gtgcgacggc ctggatcggg ccgccggcac tcgccggctt
     1561 gggcttgttg cgcaactgcg ggccatcgga gatcagggtc tcgatgtcct cgcgtgtgaa
     1621 ctcgctgagc gtgtggtgaa ccccggcctg ccccgtctcg agcaaccgga tcacgtaggc
     1681 gcccccctgc tcgaacagga agatgtcctt cggcctctgg gcgtccatcc agcggccgat
     1741 cacccgcagc gaacgctcga acgtgccggc cgcggccatc gcgggttgcg cgccgccctt
     1801 ccctcgccgc gccgcggcct cttccatgaa cttcgcgatg tcgtcgtcga agaagctcag
     1861 ggtctccttg ttgacctggc ccatggtgtc ggaccaggag ccgccggcgg tggtggccgc
     1921 aacgaggccc tggacgacaa agccgtccgg cacctccacg atgaggatgt cccgcatacc
     1981 gcggctgtcg aggaacgcgc cgatcgaccg gaagacttcc tcgaagtcct gtcgcgggct
     2041 gccgtcgtag atccgcatca gctctcctcg ctgcggcgcc ccgccgcccg gtcgacctcc
     2101 ggcacttacg agccggtcgt ccggtccagc agcccgagat cacgggcgcg acgttcgctg
     2161 gatcggaagg tggcgatggc cgcgagtgta gccagcgccc cggtcacgca caaggcaagg
     2221 acgatggtcg taccgtccgg cgccgaagta ccggtgacag atgtccacac ggaattcggg
     2281 ccgccgatcg aggacgggcc gtcgggcacg acggcgag
//
LOCUS       DBNHXO010000043         3462 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5169242, whole genome shotgun sequence.
ACCESSION   DBNHXO010000043 DBNHXO010000000
VERSION     DBNHXO010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5169242"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="AC162A_00970"
     CDS             <1..409
                     /locus_tag="AC162A_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HIR5009434.1"
                     /translation="AVRPDAHETFNPTALGRYWHERVVRFAGDHGLAQVGNSDAHSVE
                     AIGTGWTTFEGSTADDVRAAIVGGTTRHHGAFHGTTGQLGTFGRQLRKYGRDARAEVG
                     GRVRRDGTGRDHGYPGGHLRPPRFEPMDGEAGR"
     gene            406..1635
                     /locus_tag="AC162A_00975"
     CDS             406..1635
                     /locus_tag="AC162A_00975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5009435.1"
                     /translation="MKIGLVSPYVYPLPGGVTQHVRYLYENLRLRGHDVRILTSSHGL
                     QRTSEGDVIRLGKGFSMPTNGSVGTITVSPRYVSQVRELLDRERFDVLHFHEPFVPFL
                     SLVILRESQSINIGTFHAYGGWSPAYEFGSRAMKGYANRLHGRIAVSAAARHFIDRYF
                     PGDYKVIPNGVDIDRYRRAVPVARWQDGTANILFVGRFEPRKGVLDLLQAYRILRKTG
                     CQCRLLLVGGGPQEREARRYVAIRRLHGVEFLGRVSDDEKAQLFRTADVFVAPATGRE
                     SFGIVLLEAMAAGAPIVASDIHGYKGVVRRDREALLVPPREPKAIAGSIARLLREPET
                     AAAMSAAGATRAEEFSWPRVTAKVEEYYGFAIRRQAAATGSLPAHFTAPVPPSPRVPA
                     AVPSAAGQLDGSPVEVP"
     gene            complement(1637..>3462)
                     /locus_tag="AC162A_00980"
     CDS             complement(1637..>3462)
                     /locus_tag="AC162A_00980"
                     /inference="COORDINATES: protein motif:HMM:NF027518.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009436.1"
                     /translation="ILILVRSALGRLILIFGMIGLTGVLGWMALAVPPDSKASGNLIF
                     TLIMVALTLATVVVTVYHPIAWSDEEMWLAVAGPAGVGILIAVGAILLGKADQTYTVG
                     PVQFTLLEVAFAFVVLGILAYAAFGVAGRLGFGPMARAPEPDDPRSLLPPASPPAEGW
                     LRLGSWLGLPIVWMIGSLLVVPVVVYVITYIPWAMVEGHQLWPGWPPGHTGQTLVQLT
                     GEMYRYHNNLTAPHAASSPWWAWPLNLKPVWFYQGSFANSTAASIYDAGNVILWWVGI
                     PAMVFVAYQAFKRRSLALALILIGFLCQWISWARIDRAAFQYHYYTSLPFLFMALGYF
                     IAELWHGPSRRTWTVARVAAVFALFAPVILWLIRKPILCNLAGVESVSPGSQACHGDP
                     GNLVVTPAAAALAVVAVVVLIVVVRLMLNLSRPRGDGRPVGPADLLPLAIAAVVGGAV
                     IALTRMLPSDATLFSVPGIVPEFIALIIAIPLGLIALQVVTARDSRRFAAGFVLAAGA
                     WFVVLYPNIAALPLPSAFVNAYQGLLPTYLYAFQFSVNTLDRSGAISFADARFAILLV
                     FLAVACAVVAYSTWVWRMALVEGDEPAAGDTSGPEGAPGTA"
BASE COUNT          563 a         1206 c         1244 g          449 t
ORIGIN      
        1 ggccgtccgg cccgacgccc acgagacgtt caacccgacg gcgctcggcc ggtactggca
       61 cgagagggtc gtgcggttcg ccggggacca cgggctggcc caggtcggca acagcgacgc
      121 tcactcggtc gaggcgatcg gaaccggctg gaccacgttc gagggatcga ccgcagacga
      181 cgtccgagcg gcaatcgtgg ggggcacgac ccgtcaccac ggcgcgttcc acggcaccac
      241 cggccagctc ggcaccttcg ggcgccagct gcggaagtac gggcgcgacg cacgcgccga
      301 ggtcggcggc agggtccgta gggatggcac cggtcgggat cacggctatc cgggcggcca
      361 cctgcggccg ccgcgattcg agccaatgga cggggaggcc ggccgatgaa gatcgggctc
      421 gtctcgccct acgtgtaccc gctgccgggc ggggtgacgc agcacgtgcg gtatctgtac
      481 gagaatctgc gcctgcgcgg ccacgacgtc cggatcctga cttcgtcgca tggactccag
      541 cggacgtccg agggcgatgt catccgcctg ggcaagggct tctcgatgcc caccaacggc
      601 tctgtcggga cgatcacggt ctcgccgcgc tacgtgtcgc aggtgcgcga gctgctcgac
      661 cgcgagcggt tcgacgtcct ccacttccac gagcccttcg tgcccttcct ctcgctcgtc
      721 atcctgcgcg agtcgcagag catcaacatc ggcaccttcc atgcctacgg cggctggtcg
      781 ccggcgtacg agttcggctc gcgagccatg aagggttacg cgaaccgcct ccatggccgg
      841 atcgccgtgt cggcggccgc ccgccacttc atcgaccgct acttcccggg cgactacaag
      901 gtgatcccca acggcgtcga catcgaccgc taccggcggg ccgttcccgt tgcgcgctgg
      961 caggacggga cggcgaacat cctgttcgtc ggacggttcg agccgcgcaa gggggtcctg
     1021 gacctgctcc aggcgtatcg catcctgcgc aagaccggct gccagtgtcg cctgctgctc
     1081 gtcggcggcg gaccgcagga acgcgaggcc cgccgctacg tcgcgatccg ccgcctgcac
     1141 ggcgtcgagt tcctggggcg ggtctccgac gacgagaagg cgcagctgtt caggacggcc
     1201 gacgtcttcg tcgcgccggc cactggccgg gagtcgttcg ggatcgtgct gctcgaggcg
     1261 atggcggccg gggcgccgat cgtcgcctcg gacatccacg gctacaaggg cgtcgtgcgc
     1321 cgggaccgcg aggccctgct ggtgccgccg cgtgagccca aggcgatcgc agggtccatc
     1381 gcgcgcctgc tccgcgagcc cgagacggcc gcggcgatgt ccgctgcggg cgccacccgc
     1441 gccgaggaat tcagctggcc gcgggtgacg gccaaggtcg aggagtacta cgggttcgcg
     1501 atccgccgtc aggcggcggc gaccgggtcg ctgccggcgc acttcaccgc gcccgtgccc
     1561 ccgtcgccga gagtgcctgc cgccgtcccg tcggctgccg ggcaactcga cggctcgccg
     1621 gtcgaggttc cctgactcag gcggtccctg gcgcgccttc ggggccgctc gtatcccccg
     1681 ccgctggctc gtcgccctcg accagggcca tccgccacac ccaggtggag taggcgacca
     1741 cggcacaggc cacggcgagg aacaccaaca ggatcgcgaa ccgcgcgtcc gcgaacgaga
     1801 tcgccccgct gcggtcgagc gtgttgaccg agaactggaa cgcgtacagg taggtcggca
     1861 gcaggccctg gtaggcgttc acgaacgcgg acggcagggg cagcgccgcg atgttcgggt
     1921 agaggaccac gaaccaggcg ccggcggcga gcacgaagcc ggccgcgaac cggcgcgagt
     1981 cgcgcgccgt gacgacctgg agcgcgatca gcccgagcgg gatggcgatg atgagggcga
     2041 tgaactcggg gacgatcccg ggcaccgaga acagcgtggc gtccgacggg agcattcggg
     2101 tcagcgcgat gaccgcgccg ccgaccacgg ccgcgatggc gagcggcagc agatccgcgg
     2161 gaccgaccgg tcgcccgtcg ccccgtgggc ggctgaggtt gagcatcagc cggacgacca
     2221 cgatcagcac cacgaccgcc accacggcca gcgcggccgc ggccggcgtg accacgaggt
     2281 tgcccgggtc gccgtggcac gcctgcgagc cggggctcac cgactcgacg cccgcgaggt
     2341 tgcacaggat cggcttgcgg atcagccaca ggatgaccgg cgcgaacagc gcgaacacgg
     2401 ccgcgaccct ggccacggtc cacgttcgcc gcgacggccc gtgccacagc tcagcgatga
     2461 agtacccgag cgccatgaac aggaacggca ggctcgtgta gtagtgatac tggaacgcgg
     2521 cccggtcgat gcgcgcccag gagatccact ggcacaggaa gccgatgagg atgagtgcga
     2581 gggcgaggct gcgacgcttg aacgcctggt aggccacgaa caccatcgcg gggatcccga
     2641 cccaccacag gatcacgttg cccgcgtcgt agatcgaggc ggcggtcgag ttggcgaagc
     2701 ttccctggta gaaccagacc ggcttgaggt tgagcggcca cgcccaccac ggcgagctgg
     2761 cggcgtgcgg ggcggtcagg ttgttgtggt agcggtacat ctcgcccgtg agctggacca
     2821 gggtctggcc ggtgtgcccg ggcggccagc cgggccacag ctgatggccc tcgaccatgg
     2881 cccaggggat gtacgtgatc acgtagacca ccacggggac gacgagcagc gacccgatca
     2941 tccacacgat cggcaggccc agccaggacc ccaggcgcag ccatccctcg gctggtggcg
     3001 acgctggcgg cagcaggctc cggggatcgt cgggctcggg cgcacgggcc atcggcccga
     3061 atccgagcct gccggccacg ccgaacgcgg cgtaggcgag gatcccgagg acgacgaagg
     3121 cgaaggcaac ctcgagcagc gtgaactgga cgggaccgac cgtgtaggtt tggtccgcct
     3181 tgccgagcag gatcgcgccc acggcgatca ggatgccgac ccccgccggg ccggcgaccg
     3241 cgagccacat ctcctcgtcc gaccacgcga tggggtggta cacggtcacc accacggtgg
     3301 ccagcgtgag cgcgaccatg atcagcgtga agatgaggtt gccggacgcc ttgctgtccg
     3361 gcggcaccgc cagcgccatc cagccgagga cgccggtgag cccgatcatc ccgaagatga
     3421 ggatcaggcg gccgagcgcc gaacggacca ggatcaggat gc
//
LOCUS       DBNHXO010000044         5172 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4574069, whole genome shotgun sequence.
ACCESSION   DBNHXO010000044 DBNHXO010000000
VERSION     DBNHXO010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5172)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4574069"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="AC162A_00985"
     CDS             complement(<1..1534)
                     /locus_tag="AC162A_00985"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR5009437.1"
                     /translation="MDDEGQRAHEDGHHVHDPHPATVAPYGSWRSSIDIELVAGSAIA
                     LGEPWLDGDDIYWLESRSAQGGRRTLLRHTPDGSTREVTPSPFPVGNRVHEYGGGSYV
                     VDRGRIVVSSTEDGRLWRFDPDAAVDPVALTPPDGGRFADLRFDPARERLYAVRETHD
                     AAEPDRPDLVVNELVAITLDGSDGPGQALVSGADFYAAPRPSPDGSRLAWLEWDQPDM
                     PWDATRLRVADVAEDGGLGPARTAAGGPGVSIVQPAWRADGVLHYVSDESGWWNLFAL
                     DGEGGLDGPARNVAPMDAELGDPAWVFGRSSYGFADDGTVLAVGRVDGLDGLLTISVS
                     GEVEPVDAPFTEVEGLQIAGGLAILIGAGPHDGAVVARLDARTGRPAGVLARSLATPI
                     DPGLLPHAEPIAYPTSGGATARALYFPPTNDAYRAPDGELPPLIVLSHGGPTSAASSA
                     LSLDRAFFTSRGIAVVDVDYRGSTGYGRPYRDALQGQWGIVDVDDCVAAAQFLAVRGS
                     VDR"
     gene            complement(1543..2820)
                     /gene="ahcY"
                     /locus_tag="AC162A_00990"
     CDS             complement(1543..2820)
                     /gene="ahcY"
                     /locus_tag="AC162A_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR5009438.1"
                     /translation="MTNAATHVPHDVTDLALAPEGLRRIEWAEREMPVLRQIRERFER
                     ERPLAGLRIGACLHVTTETANLMRTLKAGGADVYLAASNPLSTQDVTAAALVESFGIA
                     TFARRGEDRDTYYAHLRSVADIRPTITMDDGCDLVSLLHSERRDQLGEVLAGTEETTT
                     GVIRLRAMAADNALAFPVVAVNEALTKHLFDNRYGTGQSTIDGILRATNLLIAGRNVV
                     IAGYGWVGRGIASRMDGHGAHVAVVEVDPVRALEALMDGYQVMTVAEAAPWGDLFVTA
                     TGNINVFRREHFASMKDGAIMANSGHFDAELDLPSLRAMAEGHVTDVRENVQQFDIGG
                     KKLNLIAEGRLVNLGAAEGHPAAVMDMSFANQALAAEYVAKHHSELEPRVYGVPEAID
                     AEVARLKLAAMGVVLDAMTPEQLEYVSSWQHGT"
     gene            complement(2817..3827)
                     /locus_tag="AC162A_00995"
     CDS             complement(2817..3827)
                     /locus_tag="AC162A_00995"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5009439.1"
                     /translation="MATIAVGQRSAVVARSTTDRAVLRAYLEQDRLFAAYAICDLEDR
                     EFGRTRWGIATEGDEIVAVGLEYSGITPQPLFLMGRNDGIEALLQHVVRPRVAYLAAK
                     AEALPAVASLYNLDPGPPMIRMWVDRTRFQPAPAATERLLPVEVGELNRLYQLGFGAW
                     LPSSTIAEGVYYGIRVNGKLVSAAGTHVISRTARLAVVGNVLTHDDHRGRGYAQAVTS
                     AVTAELLRYCDQVVLNVRSDNPPAVQAYRRLGYTEHCRFEERLVRRTTSPWADLTAPL
                     RRLLSRGAARGGDAQGANRTAVQHARGPANNDQAHPIDDLPLDDLSLASPSPDGPPEE
                     PR"
     gene            complement(3827..>5172)
                     /locus_tag="AC162A_01000"
     CDS             complement(3827..>5172)
                     /locus_tag="AC162A_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor eIF-2B
                     alpha/beta/delta subunit family protein"
                     /protein_id="HIR5009440.1"
                     /translation="DQPVQPPDPNRRRFFRQFAGDVMTSMSSVIGAAQLLQQESAAAA
                     RELLGEVDPEDAAPDASAASAGSPGNVAAQAVPAAGVGYRAAFRWDGDVCRVVDQRRL
                     PEMLVDLEVRGAADGVAAVRDDAVVGSLVQAQLAAVTVALVAGKTTTQRPFARRATIR
                     GAANAFRTTRPGSAAIAHTVARLLAVEERLGIDAEGEAIAAELLAEATQILGEATDAQ
                     GLVVEHALTALAELAADREDPLRVLTIGSTGAMGGGQSGTALSTIIAAHHAKRPIEAL
                     VAETRPGFDGSRIAAWELREAGVTHAVVIDAAAPGRIAAGDVDVVLVGADRIAANGDV
                     VAVAGSYPLALAASAAGVPFLVCVPTITIDPAVGDGAAAPIDEGRPGATLFAAGTRVT
                     PEGTTTRTTVQDLTPAALVTAIVTERGTLRPPFATSIAAVVVVPEPETAPVEAVG"
BASE COUNT          694 a         1886 c         1897 g          695 t
ORIGIN      
        1 gccggtcgac ggatcctcga acggcgagga attgcgccgc cgcgacgcag tcgtccacgt
       61 ccacgatgcc ccactggccc tggagcgcgt cgcgataggg acgcccgtag ccggtcgagc
      121 cgcggtaatc gacgtcgacg acggcgatgc ctcgcgacgt gaagaacgcc cggtccagcg
      181 acagcgcgga cgacgccgcg gacgtcggcc cgccgtggga caacacgatg agcggcggca
      241 gctccccgtc cggtgcgcgg taggcgtcgt tggtgggcgg gaaatacagg gcccgtgccg
      301 tggcgccacc ggacgtcggg taggcgatgg gctccgcgtg cgggagcagg cctggatcga
      361 tcggcgtcgc gagggagcgc gccaggacgc ccgccgggcg accggtccgc gcgtccaggc
      421 gggcaacgac cgcgccgtcg tgcggtcccg cgccgatcag aatggccagt ccgccggcga
      481 tctggagccc ctcgacctcg gtgaacggcg cgtcgaccgg ctcgacctcg cccgacacgc
      541 tgatcgtgag caggccgtcc agcccgtcca cgcggccgac cgccagcacg gtgccgtcat
      601 ccgcgaaccc gtaggaggac cggccgaaca cccatgccgg gtcgcccagc tcggcgtcca
      661 tcggcgcgac gttgcgagcc gggccgtcga gcccgccctc cccgtccagg gcaaacaggt
      721 tccaccagcc cgactcgtcc gagacgtagt ggagcacccc atccgcgcgc caggccggct
      781 gcacgatcga cacgcccggt ccgccggccg ccgtgcgtgc cgggccgagc ccaccgtcct
      841 ccgcgacatc cgccacgcgg agccgcgtcg cgtcccacgg catgtccggc tggtcccact
      901 cgagccaggc gagccggctc ccgtcgggcg acggccgcgg cgcggcgtag aagtcggcgc
      961 cgctcacgag cgcctgcccg gggccatcgg acccgtcgag cgtgatcgcc accagctcgt
     1021 tgacgacgag gtccggacga tcgggttcgg cggcgtcgtg cgtctcgcgc acggcgtaca
     1081 ggcgctcgcg cgccgggtcg aaccgcaggt cggcgaagcg tccgccgtcg ggcggcgtca
     1141 gcgcgacggg atcgaccgcg gcgtccgggt cgaaccgcca tagccggccg tcctcggtcg
     1201 acgagacgac gatccgcccg cgatcgacga cgtacgagcc accgccgtac tcgtggacgc
     1261 ggttgcccac cgggaacggc gacggcgtga cctcgcgcgt cgacccgtcc ggcgtgtggc
     1321 ggagcagggt ccggcggcca ccctgcgcgg agcgggactc gagccagtag atgtcgtcgc
     1381 catcgagcca cggctcgccg agcgcgatcg ccgagccggc gaccagctcg atgtcgatgg
     1441 acgagcgcca gctgccgtat ggcgcgaccg tcgccgggtg cgggtcgtgc acgtggtggc
     1501 cgtcctcgtg ggcgcgctgc ccctcgtcgt ccatgcgcgg ctctacgtcc cgtgctgcca
     1561 ggaggacacg tactcgagct gctcgggggt catcgcgtcc agcacgacgc ccatcgcggc
     1621 cagcttgagc ctcgccacct cggcgtcgat cgcctcgggc accccgtaca cgcgcggctc
     1681 gagctcgctg tggtgcttgg ccacgtactc cgcggccagc gcctggttcg cgaagctcat
     1741 gtccatgacc gcggccgggt ggccctcggc agcgcccagg ttgacgagcc gtccctcggc
     1801 gatgaggttg agcttcttgc cgccgatgtc gaactgctgg acgttctcgc ggacatcggt
     1861 cacgtgaccc tcggccatgg ctcgcagcga gggcaggtcc agctcggcat cgaagtggcc
     1921 ggagttggcc atgatcgcgc cgtccttcat cgaggcgaag tgttcgcgcc ggaacacgtt
     1981 gatgttgccg gtcgcggtga cgaacaggtc gccccacggc gcggcctcgg cgaccgtcat
     2041 cacctggtag ccgtccatca gcgcctcgag cgcgcggacc ggatcgacct cgaccaccgc
     2101 cacatgcgcg ccgtgcccgt ccatgcgcga tgcgatcccg cggccgaccc agccgtagcc
     2161 cgctatgacc acgttccgcc cggcgatcag caggttcgtc gcccgcagga tgccgtcgat
     2221 cgtcgactgg cccgtcccgt accggttgtc gaacaggtgc ttggtgagcg cctcgttgac
     2281 ggcgacgacc gggaacgcga gcgcgttgtc ggccgccatc gcgcggagcc ggatgacgcc
     2341 cgtggtcgtc tcctcggtgc ccgcgaggac ctcgcccagc tggtcgcggc gctcgctgtg
     2401 gagcaacgac acgaggtcgc agccatcgtc catcgtgatc gtcggccgga tgtcggccac
     2461 cgatcgcagg tgagcgtagt aggtgtcgcg gtcctcaccg cggcgggcga aggtcgcgat
     2521 cccgaacgac tcgacgagcg cggcggccgt cacgtcctgc gtggacagcg ggttggacgc
     2581 cgcgaggtag acatcggcac cacccgcctt gagcgtgcgc atgaggttcg cggtctcggt
     2641 cgtgacgtgg aggcacgcgc cgatgcgcaa gccggcgagc ggccgctcgc gctcgaagcg
     2701 ctcgcggatc tggcgcagga cgggcatctc gcgctcggcc cactcgatgc ggcggagccc
     2761 ctcgggggcg agggcgaggt cggtgacgtc gtgcgggacg tgggtggcgg cgttggtcat
     2821 cggggttcct caggggggcc gtcgggcgac ggcgatgcga gcgataggtc gtcgagcggg
     2881 aggtcgtcga tcgggtgagc ctggtcattg ttggccgggc cgcgggcgtg ctggacggcg
     2941 gtccggttcg ctccttgggc gtccccgcca cgcgccgctc cgcgcgagag caggcgccgc
     3001 agcggcgcgg tgaggtcggc ccagggcgag gtcgtgcgtc gcacgagccg ctcctcgaac
     3061 cggcagtgct cggtgtagcc gaggcggcgg tacgcctgga cggcgggcgg gttgtccgat
     3121 cggacattga gcacgacctg gtcgcagtag cgcagcagct ccgcggtcac ggcggacgtg
     3181 acggcctgcg catagccacg gccgcgatgg tcgtcgtggg tgagcacgtt gccgacgacg
     3241 gcgagtcgcg cggtgcgcga gatcacgtgc gtgccggccg ccgacacgag cttgccgttc
     3301 acccggatcc cgtagtacac gccctcggcg atggtgctcg acgggagcca cgcgccgaag
     3361 ccgagctggt acaggcgatt gagctcgccg acctccaccg gcagcaggcg ttcggtggcg
     3421 gccggtgcgg gctggaagcg cgtccggtcg acccacatgc ggatcatcgg cgggccaggg
     3481 tcgaggttgt agaggctcgc gacggcgggc agcgcctccg ccttcgccgc caggtacgcc
     3541 actcgcggcc ggacgacgtg ctggagcagg gcctcgatcc cgtcgttgcg gcccatcagg
     3601 aacagcggct ggggcgtgat cccgctgtac tcgaggccga ccgcgacgat ctcgtcgccc
     3661 tcggtggcga tcccccaccg cgtccggccg aactcgcggt cctcgaggtc gcagatcgcg
     3721 tatgccgcga acaaccggtc ctgctcgagg taggcgcgca ggaccgcgcg gtcggtggtc
     3781 gagcgcgcga cgacggcgct gcgctgaccg acggcgatcg tggccatcag ccgaccgcct
     3841 cgacgggggc cgtctcgggc tccggcacga cgacgacggc ggcaatcgac gtcgcgaacg
     3901 gaggccggag cgtgccgcgc tcggtgacga tcgccgtgac cagcgccgcc ggggtcaggt
     3961 cctggaccgt ggttcgcgtc gtcgtgccct cgggcgtgac gcgtgtcccg gccgcgaaca
     4021 gcgtcgctcc cggccgaccc tcgtcgatcg gcgcggcggc gccgtcgccc acggccgggt
     4081 cgatcgtgat cgtcggcaca cagacgagga acggcacgcc ggcggccgat gccgcgagtg
     4141 caagtgggta gctgcccgca acggcgacga catcgccgtt ggccgcgatg cggtcggcgc
     4201 cgaccagcac gacatcgaca tcgccggccg cgatccgacc cggcgcggcg gcgtcgatga
     4261 ccacggcatg cgtgacgccg gcctcgcgca gctcccaggc ggcgatccgg gaaccgtcga
     4321 atccggggcg cgtctcggcg accaatgcct cgatcgggcg cttcgcgtgg tgggcggcga
     4381 tgatcgtcga gagcgccgtc ccgctctggc cgccgcccat ggcccccgtg ctcccgatcg
     4441 tgaggacgcg cagcgggtcc tcacgatcgg ccgcgagctc ggccagcgcc gtcagcgcat
     4501 gctcgaccac gagaccctgc gcgtcggtgg cctccccgag gatctgggtc gcctcggcca
     4561 gcagctcggc cgcgatcgcc tctccctcgg cgtcgatccc gagacgctcc tcaaccgcga
     4621 gcagccgcgc cacggtgtgc gcgatcgccg ccgagccggg gcgcgtcgtg cggaatgcgt
     4681 tggccgcgcc ccggatcgtc gcccggcgcg cgaacggccg ctgcgtcgtc gtcttgcccg
     4741 ccacgagtgc cacggtcacc gcggcaagct gggcctgcac caacgagccg acgacggcgt
     4801 cgtcgcggac cgcagcgaca ccgtcggcag cgccccggac ctcgagatcc accagcattt
     4861 ccggcagcct gcgctggtcc acgacccggc acacgtcgcc gtcccagcgg aacgcggccc
     4921 ggtaccccac cccagcggcc ggtaccgcct gggccgccac gttccccggg gaaccggcac
     4981 tcgcggcgga ggcgtcgggc gccgcatcct ccggatcgac ctcgcccagc agctcgcgcg
     5041 ccgccgcggc cgactcctgc tggagcagct gcgcggcgcc gatgaccgag ctcatcgacg
     5101 tcatcacgtc gcccgcgaac tgccggaaga accggcgacg gttcgggtcg ggcggctgga
     5161 cgggctggtc gg
//
LOCUS       DBNHXO010000045         7134 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_408443, whole genome shotgun sequence.
ACCESSION   DBNHXO010000045 DBNHXO010000000
VERSION     DBNHXO010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 7134)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7134)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7134
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_408443"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..2751
                     /locus_tag="AC162A_01005"
     CDS             199..2751
                     /locus_tag="AC162A_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009441.1"
                     /translation="MAKVPRLLRREPAIDKAELELARSRSLERLKQITASRHQADAAP
                     DAAADRANGSASVAVADPSRPSIEIVGSASVDAPQRSGATNGSLPHWGASLVGVMDEA
                     TADTASDVEAEQDDVAGIAQDLDHEAVEPETAEADATVESLAIEPDDEAVAVIELPSP
                     ADLADGDSVEFASEPIDNADLTAVVLDVADASWVSNGTHDEVTVDIVPWPEASDGDVV
                     ADESAKRAADEEDAREAADRAAAEAAWAEAMAAADQAAAEDAARAETFAAERTAAEHR
                     RTEARAAEQARAAAEVAERDAAEHAWAAAMAAAEQAAADERARAEALAAEQARAAEKA
                     RAAEERARAEALAAEQAAADRAAVERAAAEQARIAEQERFAEQRARAEALAAEERARE
                     EERARAEALAAAALAAQLARVAEAKARAEEQARAERQARAEAARAAEEQARAEAAEQA
                     RIAAEQAAAEDQARAEEQARIAAEEQARAEEQARAEAQARAEAQARAEALAAAELART
                     EKRARAEARAAELAAADQARAAQVERAAAEQAELEAAAARAATVDVDEIVEVSHNGHA
                     SEVDEVADLEAGWPAEMPDLAPLPREKAGKTRSSSSKAARKAARKAAPQVPSQASCPY
                     CAHLLVPVPTANRRCDRCRQKIMVRHVDDRVVFLTEASVLVFESERRRIADSRNWVGP
                     RNQWTRLAASAGAPEDKVARLNETLASPETVEAARNLYLAAVDRSVREARQERDWPGA
                     AEIRRAQAAAMYKVAGSPETLPADVIALHRDAAAIELRGIAELARDAELVGGSCCEAC
                     AANDGRSFRIGPDLRSYLPHESCPNGVCSCRWRLSDRDRAAVERLLRSGAKP"
     gene            complement(2797..4017)
                     /locus_tag="AC162A_01010"
     CDS             complement(2797..4017)
                     /locus_tag="AC162A_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE/TadZ family protein"
                     /protein_id="HIR5009442.1"
                     /translation="MQLSILLLATDRVAGEALTTALAGPGHGVTTVADPVDLFASAAG
                     YNLVIIDKVPPPFTVAAVIEELRRDDAASTIPVLAVAQGDDIEERIALLEAGADDVIT
                     KPFDHTELEARVEALSLRFQRSQMRPNIVAVTAIGDPRGRRIVSVYSPKGGVGATTIA
                     TNLALLAGERHPDRTLLIDLDLSFGQVSSHMNLQPKQTLLELARDEAALREAELFRTY
                     AIQHPSGIHVLAAPTSPGYAVLITADTVEVVLERALEAYDVVIVDAGTALDERMLTVF
                     SRSDTVVIPVLPEIPALNAVHLLLEQLTETGSLGASTVFVLNNLFARELLKRADIEAA
                     LGAPISASLPYDSIAYLKAVNEGSPVVRSAPKSVAAASLRSLADTILGPVATAGSASG
                     GGKGEKKGRFGRSR"
     gene            4192..4638
                     /gene="rpsF"
                     /locus_tag="AC162A_01015"
     CDS             4192..4638
                     /gene="rpsF"
                     /locus_tag="AC162A_01015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR5009443.1"
                     /translation="MRRYELMLVLRPDAPDERAAAVIDRTTRYVVAEGGQIVKVAPWG
                     RRRLAYPIDRYREGSYHIVVFEAPAESIAELERSLQITEEVLRYLVTRTIKPIKARRD
                     AAGGVDIEDIDVDDADIPLLDEDVEDEDTGELIDESESEAAPAAIE"
     gene            4654..5148
                     /locus_tag="AC162A_01020"
     CDS             4654..5148
                     /locus_tag="AC162A_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR5009444.1"
                     /translation="MASFSKAMIIGNLGRDPEMRYTPNGRPVTEFSVAVNQSTKNQQT
                     GEWVEATDWFRVTVWGDRAERTAEQLRKGNRVFVEGRFRTREFEGRDGQKRISLEITA
                     DNVVSLEARARDEEGSFAAPTGGFGAPGGGGSGGGGDEPPVRPTRPSGGPSGGDDDLD
                     DLPF"
     gene            5172..5435
                     /gene="rpsR"
                     /locus_tag="AC162A_01025"
     CDS             5172..5435
                     /gene="rpsR"
                     /locus_tag="AC162A_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR5009445.1"
                     /translation="MPPKSKKRDDYYRDRRRRKVCSFCADKTVSIDYKEVNRLRRYLS
                     ERAKIESRRKTGTCAAHQRELSVALKRARHVALLPYAPQHLRP"
     gene            5440..6318
                     /locus_tag="AC162A_01030"
     CDS             5440..6318
                     /locus_tag="AC162A_01030"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR5009446.1"
                     /translation="MTAGLLLVAGGAAAGMFGSLLGLGGGLLLVPLLTLAFGVPLREA
                     VAISLVAVIVTSTAGAGVYLRRHVANLRLGMSLELFTAIGALAGGLIAFVLDEQTLAG
                     LFAVVLTWVAVSMIRQDRLTTDEAPADPEREHEPETIVPDAPAGLPAAEVVAPPATFT
                     DSLAGDGYRVHRLGFGMVASVGAGLVSALLGVGGGIIKVPVMNVAMGVPLKVATATSN
                     MMIGITATSSAVIYLLRGEIDPFIAGPTALGVFVGASVGSRVAGRIDVRVLRWLFVLV
                     VLYTAFEMARRALGAG"
     gene            6315..6716
                     /locus_tag="AC162A_01035"
     CDS             6315..6716
                     /locus_tag="AC162A_01035"
                     /inference="COORDINATES: protein motif:HMM:NF019459.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HIR5009447.1"
                     /translation="MSAQRVPGAPGASSGAAYAIETIVARMLVIGTWVAVGLILVGVL
                     GMIASGIDPMAHPPVAAFDPAAIPGDLVALRPAGFIWTGILVVMALPIGRVVVAGAGF
                     YAAGERRLALISVLVIVVVGLSIVAASRIGV"
     gene            6716..>7134
                     /locus_tag="AC162A_01040"
     CDS             6716..>7134
                     /locus_tag="AC162A_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HIR5009448.1"
                     /translation="MDIAILIIAFLVILLGAELFTNGIEWFGRKLNLAQGAVGSVLAA
                     VGTALPETMIPVIAIIFGGGGEATHAVGVGAILGAPFMLSTLAMFVTGIGVIAFRKRR
                     ATDTRMVVDTEVLVHDIRYFLIAYGLAIATAFLPPDL"
BASE COUNT         1050 a         2476 c         2643 g          965 t
ORIGIN      
        1 ccagccatcc ccgcgagcca tggccggtcg gcctcgtcgt cgttgccagg tgacaacgat
       61 cgcaagaacg ggtagtgctt ttgggctatt cgtgtggtat taccctctat cccagtccag
      121 tgcggcaggg tccggttggg gggagtgggg atgggaccgg cccgaggcag ccggctgcac
      181 gtcgaggtgg ttcgggacat ggcgaaggtg ccgcgactcc tgcgcaggga gccggcgatc
      241 gacaaggcgg agcttgagct cgcccggtcg cgctcgctcg agcgcctcaa gcagatcacg
      301 gcgtctcgcc accaggcgga cgccgcgccc gacgctgcgg cagatcgggc caacggcagc
      361 gcatcggtcg ccgtcgcaga cccttcgcgg ccatcgatcg agatcgtcgg gagcgcgtcc
      421 gtggacgcgc ctcagcggtc cggagccacc aacgggtcgc tgccccactg gggcgcgtcg
      481 ctcgtcggcg tcatggacga ggccacggcc gacaccgcgt cggacgttga ggctgaacag
      541 gacgacgtcg cgggaattgc gcaggacctc gaccacgagg ccgtcgagcc cgagacagcc
      601 gaggccgacg cgaccgtcga gtccctcgcg atcgagcccg acgatgaagc cgtcgcggtc
      661 atcgaactgc cgagcccggc cgacctcgcg gacggcgaca gcgtcgagtt cgcgagcgag
      721 ccgatcgaca acgccgacct gaccgccgtc gtcctcgacg tcgccgatgc gagctgggtc
      781 tcgaacggga cccacgacga ggtcaccgtg gacatcgtcc cgtggcccga ggcgtccgac
      841 ggtgacgtgg tggccgacga gtccgcgaag cgtgccgccg acgaggagga tgcccgggag
      901 gccgccgatc gagccgcggc cgaagcggca tgggccgagg cgatggccgc cgccgaccag
      961 gccgccgcgg aggatgccgc gcgcgccgaa accttcgccg ccgagcggac cgccgccgag
     1021 cataggcgga ccgaggctcg cgccgccgag caggctcggg ccgccgccga ggtcgctgaa
     1081 cgcgacgccg ccgagcacgc ctgggcggcc gcgatggccg ccgccgagca ggccgctgcc
     1141 gatgaacggg cacgcgccga ggccctggcg gccgagcagg cccgggcggc cgagaaagcc
     1201 cgggcagcgg aggaacgggc acgcgccgag gccctggcgg ccgagcaggc cgcagccgat
     1261 cgcgccgccg tcgaacgggc tgcggccgag caggcgcgca ttgccgaaca ggaacggttc
     1321 gccgagcagc gggcgcgtgc cgaggcgctc gcggcggagg agcgggcgcg agaggaagag
     1381 cgggcgcgag cggaagcgct cgccgcggca gcgctggccg cccagctggc ccgggtcgcg
     1441 gaagccaagg cacgcgccga ggagcaggcc cgagcggaaa ggcaggcccg agccgaagca
     1501 gctcgtgcgg ccgaggaaca ggcacgggcc gaggcggccg agcaggcgcg catcgccgcc
     1561 gagcaggccg cggccgagga ccaggcacgg gccgaggaac aggcacgcat cgcggccgag
     1621 gagcaggcac gggccgagga acaggcacgg gccgaagcac aagcacgggc cgaagcacaa
     1681 gcacgggccg aagccctggc cgccgccgag ctggcacgga cggagaagcg cgctcgcgcc
     1741 gaagcacggg ctgccgagct cgccgccgcc gatcaggccc gcgccgcgca ggtcgagcgg
     1801 gccgccgccg agcaggccga gctcgaggcc gcggccgcac gtgctgcgac cgtcgatgtc
     1861 gacgagatcg tcgaggtctc ccacaacggg cacgcgagtg aggtcgacga ggtcgccgac
     1921 ctggaggccg ggtggccggc cgagatgccg gacctcgcgc ccctgccccg ggagaaggcc
     1981 gggaagacgc gctcgtcgag ctccaaggcg gcccgcaagg cggcccgcaa ggccgcgccg
     2041 caggtcccgt ctcaggcgtc ctgcccgtac tgcgcccacc tgctggtgcc cgtcccgacc
     2101 gctaaccggc gatgcgatcg ctgtcgccag aagatcatgg tccgccatgt cgacgaccgc
     2161 gtggtcttcc tcaccgaggc ctcggtgctc gtcttcgaat ccgagcgtcg gcgcatcgcc
     2221 gacagtcgga actgggtcgg gccgcggaac cagtggacgc gcctcgccgc ctcggcggga
     2281 gcaccggagg acaaggtcgc caggctcaac gagaccctcg cgtcgccgga gacggtcgag
     2341 gccgcccgaa acctctatct cgcggcggtc gaccgctcgg tgcgagaagc acgacaggag
     2401 cgcgactggc ccggcgccgc cgagatccgg cgggcccagg cggccgcgat gtacaaggtc
     2461 gccgggtcac ccgagacgct cccggccgac gtcatcgcgc tgcaccggga cgccgccgcg
     2521 atcgagctcc gcgggatcgc ggagctggct cgggatgccg agctggtcgg cgggtcgtgc
     2581 tgcgaggcgt gcgcggcgaa cgacggtcgc tcgttcagga tcggccccga cctgcggtcg
     2641 tacctgccgc acgagagctg cccaaacggc gtctgctcgt gccggtggcg cctgagcgat
     2701 cgggatcggg ccgccgtcga gcgcctgctg cggagcggcg ccaagccctg atcgcggcga
     2761 gtcgcgtcga ggtcccgggt cagagccggc gccgggtcaa cgggagcgcc cgaaccgccc
     2821 cttcttctcg cccttgccgc ccccggacgc cgacccggcg gtcgcgaccg ggccgaggat
     2881 cgtgtcagcg agggaccgga ggctcgccgc cgcgaccgac ttcggggcgc tgcgcacgac
     2941 cgggctgccc tcgttgacgg ccttgaggta cgcgatcgag tcgtacggca gcgatgcgct
     3001 gatcggcgcg ccgagcgccg cctcgatgtc cgcgcgcttc agcagctcgc gggcgaacag
     3061 gttgttgagc acgaacaccg tcgacgcgcc gagcgacccc gtctcggtca actgctcgag
     3121 cagcaggtgc acggcgttga gcgccgggat ctcggggagg acggggatga cgacggtgtc
     3181 cgagcggctg aagaccgtga gcatccgttc gtcgagggcc gttccggcat cgacgatgac
     3241 gacgtcatac gcctcgagcg cccgctcgag gacgacctcg acggtgtccg cggtgatcag
     3301 gacggcgtag cccggcgacg tgggtgcggc cagcacatgg atcccgctcg ggtgctggat
     3361 cgcataggtc cggaacagct ccgcctcgcg cagcgccgcc tcgtcgcggg cgagctcgag
     3421 gagcgtctgc ttcggctgca ggttcatgtg cgacgagacc tggccgaacg agaggtcgag
     3481 gtcgatcagg agcgtccggt cggggtggcg ctcgcccgcc agcagggcga ggttcgttgc
     3541 gatcgtcgtg gcgcccacgc cgcccttcgg gctgtacacg gacacgatcc ggcggccgcg
     3601 cggatcgccg atcgcggtga cggcgacgat gttcgggcgc atctgcgacc gctggaaccg
     3661 gagcgagagg gcctcgacgc gtgcctcgag ctccgtgtgg tcgaagggct tggtgatgac
     3721 gtcgtcggcg ccggcctcga ggagggcgat gcgctcctcg atgtcgtcac cctgggcgac
     3781 ggcgagcacc gggatggtcg acgcggcgtc gtcccgtcgg agctcctcga tcacggccgc
     3841 gaccgtgaac ggcggcggca ccttgtcgat gatcacgagg ttgtagcccg cggccgacgc
     3901 gaacaggtcg accgggtcgg ccacggtcgt cacgccgtgt ccgggtccgg cgagggcggt
     3961 ggtcagggcc tcgccggcga cgcggtcggt cgcgaggaga aggatcgaga gctgcaacgt
     4021 attccccgag gctggtcggt cgacaacgac cggaacgaca tcggcgcgaa ggatacaggg
     4081 tcgcatgcag ggccgggggt gggtctcgtg ctatcctgcc gccgtccttt ccgccccgcg
     4141 ccggcatgcc gtcagctggg gcagatccca ttggaaggag tacagctggc cgtgcgccgc
     4201 tatgaactca tgctcgtcct ccggcccgat gcgccggatg agcgggccgc cgcggtcatc
     4261 gaccgcacca cacgctacgt cgtcgccgag ggcgggcaga tcgtcaaggt cgctccctgg
     4321 ggtcgtcgcc ggctcgcgta tcccatcgat cgctaccgcg agggctcgta ccacatcgtg
     4381 gtattcgagg ctcccgccga gtcgatcgcg gagctggagc gaagcctgca gatcaccgaa
     4441 gaggtgctcc gctacctcgt cacccgcacg atcaagccga tcaaggcgcg ccgcgacgcg
     4501 gccggcggcg tggacatcga ggacatcgac gtcgacgacg ccgacatccc gttgctcgac
     4561 gaggacgtcg aggacgagga cacgggcgag ctgatcgacg agtccgagag cgaagccgcg
     4621 cccgcggcca tcgagtagag cagggagccg gcaatggcgt cgttttcgaa ggccatgatc
     4681 atcggcaacc tcgggcgcga tcccgagatg cggtacacgc ccaacggacg tccggtcacg
     4741 gagttcagcg tcgcggtcaa ccagagcacg aagaaccagc agaccggcga gtgggtcgag
     4801 gcgaccgact ggttccgggt caccgtctgg ggtgatcgcg ccgagcggac cgccgagcag
     4861 ctgcgcaagg gcaaccgtgt gtttgtcgag ggccgcttcc ggacccggga attcgagggc
     4921 cgcgacggcc agaagcggat ctcgctcgag atcaccgcgg acaacgtcgt cagtctcgag
     4981 gcccgcgccc gcgacgaaga gggttcattc gcggcaccga ccggtggctt cggcgccccg
     5041 ggtggtggcg gcagcggcgg cgggggcgac gagccccccg ttcggccgac ccgtccctcc
     5101 ggcggaccct cgggcggcga cgacgacctc gacgacctcc ccttctaacg atcttccagg
     5161 agaactgacg aatgcccccc aagtcgaaga agcgcgacga ctactaccgc gaccggcgtc
     5221 ggcgcaaggt ttgctccttc tgcgcggaca agacggtctc gatcgactac aaggaggtca
     5281 accgcctccg ccggtatctg tccgagcgcg ccaagatcga gtcgcgccgc aagaccggga
     5341 cgtgcgccgc gcaccagcgc gagctgtccg tcgccctgaa gcgggcccgg cacgtggcgc
     5401 tgctcccgta cgcgccccag cacctccgcc cgtagcgcgg tgaccgcggg cctgctgctt
     5461 gttgcgggag gcgccgcggc gggcatgttc ggctcgctgc tcgggctggg cggcggcctg
     5521 ctgctggtgc cgctgctgac cctcgccttc ggcgtcccgc tgcgcgaggc cgtcgcgatc
     5581 tcgctggtcg cggtcatcgt gacctcgacg gccggcgcgg gcgtctatct gcgccgccac
     5641 gtcgcgaacc tgcggctggg catgtcgctc gagctgttca cggcgatcgg cgccctggcc
     5701 ggcggcctga ttgcgttcgt gctcgacgag cagaccctcg ccgggctgtt cgcggtggtg
     5761 ctcacctggg tggcggtctc gatgatccgg caggaccgcc tcacgaccga cgaggcgccg
     5821 gccgatcccg aacgggagca cgaacccgag acgatcgtcc cggacgcgcc cgctggcctt
     5881 ccggccgccg aggtcgtcgc tccgccagcg accttcaccg acagcctcgc gggcgacggc
     5941 taccgcgtcc accggctggg cttcgggatg gtcgcctcgg tgggcgcggg gctcgtgtcc
     6001 gcgctgctgg gcgtcggcgg cggcatcatc aaggtgccgg tgatgaacgt cgcgatgggc
     6061 gtgccgctca aggtggcgac ggccaccagc aacatgatga tcgggatcac ggcgacgtca
     6121 tcggcggtga tctacctgct ccgcggcgag atcgacccgt tcatcgccgg gcctacggca
     6181 ctgggcgtgt tcgtcggcgc ctcggtcggg tcgcgcgtcg cgggccggat cgacgtgcgc
     6241 gtcctgcgct ggctgttcgt gctcgtggtg ctgtacaccg cgttcgagat ggcgcgccgc
     6301 gccctgggag ccgggtgagc gcgcagcgcg tccccggagc gcctggcgcc tcgagcgggg
     6361 ccgcctacgc gatcgagacc atcgtggccc ggatgctcgt catcggcacg tgggtcgcgg
     6421 tggggctgat cctcgtcggg gtgctcggaa tgatcgcctc gggcatcgac ccgatggcgc
     6481 atccgccggt tgcggcgttc gatccggcgg ccattccggg cgacctggtg gcccttcggc
     6541 cggccggatt catctggacc ggtatcttgg tcgtcatggc actccccatc gggcgggtgg
     6601 tcgtggccgg tgcggggttc tacgccgccg gggaacgacg actcgcgctg atctccgtac
     6661 tcgtgatcgt ggtcgtcggg ctatcgatcg tcgccgcctc gaggatcggc gtctgatgga
     6721 catcgcgatc ctcatcatcg cgttcctcgt catcctgctc ggcgccgagc tgttcaccaa
     6781 tggcatcgag tggttcgggc gcaagctcaa cctggcccag ggtgcggtcg gctcggtgct
     6841 cgccgcggtc gggacggcgc tgcccgaaac gatgatcccg gtcatcgcga tcatcttcgg
     6901 cggcggcggc gaagcgaccc acgcggtcgg cgtcggcgcg atcctgggcg ccccgttcat
     6961 gctgtccacg ctggccatgt tcgtgaccgg tatcggcgtg atcgcgttcc gcaagcggcg
     7021 tgcgaccgac acccggatgg tggtcgacac cgaggtgctc gtccacgaca tccgctactt
     7081 cctgatcgcc tacgggctcg cgatcgcgac cgcgttcctg ccgcccgacc tgaa
//
LOCUS       DBNHXO010000046         2794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_805635, whole genome shotgun sequence.
ACCESSION   DBNHXO010000046 DBNHXO010000000
VERSION     DBNHXO010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_805635"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="AC162A_01045"
     CDS             complement(<1..724)
                     /locus_tag="AC162A_01045"
                     /inference="COORDINATES: protein motif:HMM:NF015427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HIR5009449.1"
                     /translation="MDARASEPGFWDDQRAAQKVLREAEGLREETALWRGLEKRADDL
                     TELLTLLDDTPDPDTLADLEREADQLDREFARERTRLLFSGEYDAKNAVLTISAGAGG
                     TEATDWAEMLLRMYLRWGERHRFRTDVVDSTEGEQAGIKSATVTMDGRFAYGWLRAER
                     GVHRLVRISPYDAQKRRQTTFALVEVMPEADEDVEIELNWDEIRVDTFRSSGAGGQHV
                     QKTESAIRLTHLPTGIVVTCQNE"
     gene            complement(1261..1863)
                     /locus_tag="AC162A_01050"
     CDS             complement(1261..1863)
                     /locus_tag="AC162A_01050"
                     /inference="COORDINATES: protein motif:HMM:NF014724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR5009450.1"
                     /translation="MRLIIVAALAVVIVASLATLRIWQQGDRDEQRPVDAIVVLGAAQ
                     YDGRPSPVFEARLDHAVRLWQSGIARSFVVTGGKLPGDRTTEAAVARRYAVDHRVPEG
                     AIFGENDAHNTLDSLRAVASEMRQLGMTSAVFVSDPTHMLRVLRIARDLGIEAYGSPT
                     PTSPIQQDLGRRVSATIHELGALVVYFISGGAPDVEQQGT"
     gene            complement(1887..>2794)
                     /locus_tag="AC162A_01055"
     CDS             complement(1887..>2794)
                     /locus_tag="AC162A_01055"
                     /inference="COORDINATES: protein motif:HMM:NF019155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HIR5009451.1"
                     /translation="EFDDVINKQRETIYAERDKVLRNEDLTETVREFLDAEMDALGDQ
                     FAGDASPSEWNFEGLSLALHAMGIEGEGTSEADLDEAGRSREELVEHLRQLADEQLTT
                     RAAEHGDEVWSQVERFVLLRTIDSLWVEHLTEVDDMRRGIGLRGYAQQDPLNEFRKEA
                     YQLYEELSSLIQHQVASTIFRVTVQREPATVPLPGPGQPALAGTSDGGDGTNGSNAVS
                     GAAPRPAVVGASLAAGGAALRRPETAPIAGVPADPMRGQRSVPGDAAASGGVRQGFTP
                     TGARIGRNEPCWCGSGQKYKKCHGA"
BASE COUNT          373 a          974 c          992 g          455 t
ORIGIN      
        1 gctcgttctg gcaggtcacg acgatgccgg tgggcaggtg cgtcagccgg atcgcggact
       61 cggtcttctg gacgtgctgg ccgccggcac ccgaggagcg gaacgtgtcc acgcggatct
      121 cgtcccaatt gagctcgatc tcgacgtcct cgtctgcctc gggcatgacc tcgacgaggg
      181 cgaatgtcgt ctgacgccgc ttctgggcgt cgtacgggct gatccggacc aggcggtgca
      241 cgccgcgctc ggcgcgcagc cagccgtacg cgaagcgacc gtccatcgtc acggtcgcgc
      301 tcttgatccc ggcctgctcg ccctcggtgg agtcgacgac gtcggtccgg aaccggtggc
      361 gctcgcccca gcgcaggtac atgcgcaaca gcatctcggc ccagtcggtg gcctcggtgc
      421 cgcccgcgcc cgcgctgatc gtcagcaccg cgttcttcgc gtcgtactcg ccggagaaca
      481 ggaggcgcgt ccgctcgcgc gcgaactcgc ggtcgagctg gtcggcctca cgctcgaggt
      541 ccgcgagcgt atcggggtcg ggcgtgtcgt cgaggagggt cagcagctcc gtgagatcgt
      601 cggcccgctt ctcgaggccc cgccacaggg cggtctcctc gcgcaggccc tccgcctcgc
      661 ggagcacctt ctgcgcggcg cgctggtcgt cccagaagcc gggctcgctg gcgcgcgcgt
      721 ccagctcagt gaggcgcgct tccttggtgg cgaggtcaaa gacgccccga ggtcgaccgg
      781 atccgctcgg ccaggtcgcg gatggacgcg gcgtccatca gtcgcgcacc tggatgtcct
      841 ggagcaggat cggacggagc tcgatcagcc gcagggcggt cttgctccgg gcgacgacgg
      901 ggttcacgca gggacagtag ctgttatggg ggcagacacc gcagttgcca tcgcaatcaa
      961 gcgcgatcgc gtaactgcag ttccggcaat cccgctcgca ggcgatcagg tctacgaact
     1021 gggcttcctc ggcctgcttc acaaagctcc ctcgctctcg tccttcgctg tcggtcgagc
     1081 gtgacggttg ggatgcagcg ccaaggctgg gcaggggcga aaggctgggc ggcgccggtc
     1141 cctgggtggc gaaggactga tcgggagtat aggcgggggt cacgctcacg ccaaggggta
     1201 gaaccggaat ttcgtgcgga agtttgcgtg ggattgaaca ccctgccgcc gggtgcgaga
     1261 tcaggtgccc tgctgctcca cgtcgggcgc gccgccggag atgaagtaca cgaccagcgc
     1321 accgagctcg tggatggtcg cggacacccg gcggcccagg tcctgctgga tcggcgaggt
     1381 cggcgtgggc gagccgtacg cctcgattcc caggtcgcgc gcgatccgca ggacccgcag
     1441 catgtgggtc ggatcggaca cgaacactgc ggacgtcatg cccagctggc gcatctccga
     1501 cgcgacggct cgcagcgagt cgagcgtgtt gtgggcgtcg ttctcgccga agatcgcgcc
     1561 ctcgggtacc cggtggtcga cggcgtagcg acgggccacc gcggcctccg tcgtgcgatc
     1621 gccgggcagc ttgccgccgg tcacgacgaa cgacctggcg atgcccgact gccacagccg
     1681 gacggcgtgg tcgaggcggg cctcgaacac cggcgacggg cggccatcgt actgggcggc
     1741 gccgagcacc acgatggcgt cgacgggccg ctgttcgtcg cggtcgccct gctgccagat
     1801 gcggagggtc gcgaggctcg cgacgatgac caccgcaagc gcggcgacga tgatgaggcg
     1861 cacgagttct ttccgcccgg accggatcag gccccgtggc acttcttgta cttctggccc
     1921 gatccgcacc agcagggttc gttgcggccg atgcgggcgc cggtgggggt gaacccctgg
     1981 cgcacgccgc cgctcgcggc ggcgtcgccc ggcacggagc gctgtccgcg catcgggtcc
     2041 gcgggcaccc cggcgatcgg cgccgtctcg gggcgccgga gcgccgcgcc gccggcggcg
     2101 aggctcgccc cgacgaccgc cggccgcgga gccgcgcccg agacggcgtt cgacccgttc
     2161 gttccgtcgc cgccgtcgct cgtgcccgcc agggccggtt gccccggtcc gggcagcggc
     2221 acggtcgccg gttcgcgctg tacggtgacc cggaagatcg tgctcgccac ctggtgctgg
     2281 atgaggctgc tcagctcttc gtacagctga taggcctcct tccggaactc gttgagcgga
     2341 tcctgctgcg cgtacccacg cagcccgatg ccgcggcgca tgtcgtcgac ctcggtcagg
     2401 tgttcgaccc acaggctgtc gatcgtgcgc agcagcacga agcgctcgac ttggctccag
     2461 acctcatcgc cgtgctctgc ggcgcgcgtc gtcagctgct cgtcggcgag ctggcgcagg
     2521 tgctcgacca gctcctcccg gctcctcccg gcctcgtcga ggtcggcctc cgacgttccc
     2581 tcaccctcga tccccatggc gtgcagggcg agcgacaacc cttcgaagtt ccactcgctg
     2641 ggcgacgcgt cgccggcgaa ctggtcgccc agggcgtcca tctcggcgtc gaggaactcg
     2701 cgcacggtct cggtcaggtc ctcgttgcgc aggaccttgt cgcgctccgc gtagatcgtc
     2761 tcgcgctgct tgttgatgac gtcgtcgaac tcga
//
LOCUS       DBNHXO010000047         3260 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1401879, whole genome shotgun sequence.
ACCESSION   DBNHXO010000047 DBNHXO010000000
VERSION     DBNHXO010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1401879"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="AC162A_01060"
     CDS             complement(<1..1028)
                     /locus_tag="AC162A_01060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5009452.1"
                     /translation="MSGTAATGSAPEAASSEAGGRPGLRGSLVMAAAAVAARLPEAPL
                     GAAAESAGELWYRVAPARRAQARANLARVCEGLAASGRGPSAARRAATDPDALERLVR
                     ASFRHAARYYLEVARAGAYDIETALARVDLDDEDEVRRTLQAGTPVIIVGMHFGAIEL
                     PVVLLSHLVGHAVTAPMEAVTDPALQRWFVSSRSRVGVNIVPIANARRSLLRALRGGE
                     SVGLVADRDLTHSGIDVPFFGHPAPIPAGPSLLALETGATIYVSAARRTRDGRYRARL
                     IRVPTPEIGTRRERMVTLTTAIASGFESILADGPEQWWGAFHPIWPDLDQTATADRRA
                     AADGLPDVP"
     gene            complement(1025..2005)
                     /locus_tag="AC162A_01065"
     CDS             complement(1025..2005)
                     /locus_tag="AC162A_01065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5009453.1"
                     /translation="MSQGRPLEAPHRRPQTSSPRIVERTAIVGYKATAAVLAHVPPRL
                     SEAVIGTAAQGSYLLWPAKRRFSNANFGHVLGLPPEHPRVRRLALRAYREYARYVVEL
                     MRLPSRPQAELVAGVEDAGVDRLIADWRASGRPMIVVAGHVGSNEAVGAALAGRGYPV
                     SVIADDSSYPELFELLSRQREDWGVKIIAWRNLRGLYSVLKRKEILALLVDWGYRSDG
                     IPVRLFGTWTTLPAGPAALAAKSGAMIAPLAIRRIGDGRVRVEPYETFTVPSSDPADI
                     QRATQRIADALESIIALAPEQWYSFKPMWPDDPAETRDLEARATALVASP"
     gene            complement(2002..3213)
                     /locus_tag="AC162A_01070"
     CDS             complement(2002..3213)
                     /locus_tag="AC162A_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018142406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HIR5009454.1"
                     /translation="MSTVNGRANGAARNGARDDKVRVAIVGVGNCASSLVQGRYYYEN
                     AKAGDFVPGLMHVQLGDYHISDVEFVAAFDVDKEKVGKDLSEAIYSGQNNTFKFMDVP
                     HLGVKVDRGMTHDGLGKYLSQVIEKAPGPTADVVGILKERQVDVMVSYLPVGSEEATK
                     WYVEQALKAGVGFVNAIPVFIGREPYWQRRFAEEGLPVIGDDIKSQVGATITHRVLTR
                     LFMDRGVRIDRTYQLNFGGNTDFLNMLERERLESKKISKTNAVTSMLDYEIADEDIHV
                     GPSDYVPWLKDRKWCHIRIEGTTFGDVPLNAELKLEVWDSPNSAGVIIDAIRCLRIGL
                     DRGLKGTLVAPSSYFMKSPPLQIHDDIAHQRVEAFIRGEDNETLVGNEVSRKDAKALA
                     GVAKAKAAATR"
BASE COUNT          456 a         1127 c         1174 g          503 t
ORIGIN      
        1 ggcacgtccg ggaggccgtc ggctgccgca cgacggtcgg ccgtcgccgt ctggtccagg
       61 tctggccaga tcgggtggaa cgcgccccac cactgctcgg ggccatcggc gaggatcgac
      121 tcgaagcccg acgcgatggc cgtcgtcagg gtcaccatac gctcgcggcg tgtcccgatc
      181 tccggcgtcg gcacccggat caggcgggcg cggtagcgac cgtcgcgagt ccgcctggct
      241 gcgctgacgt agatcgtggc gcccgtctcg agcgccagca gcgagggccc ggcggggatc
      301 ggtgccgggt ggccgaagaa cgggacgtcg atgcccgagt gggtcaggtc gcgatcggcg
      361 accagaccga ccgattcccc tccgcggagc gcgcgcagca gggagcggcg ggcgttggcg
      421 atgggcacga tgttgacccc gacacggctg cgcgagctca cgaaccagcg ctgcagggcc
      481 ggatcggtga cggcctccat cggcgctgtc acggcgtggc cgacaagatg tgagagcagg
      541 accaccggca gctcgatcgc cccgaagtgc atgccgacga tgatgacggg ggtgcccgcc
      601 tggagcgtcc gtcgcacctc gtcctcgtcg tcgaggtcta cgcgcgccag cgctgtctcg
      661 atgtcgtagg cgcccgcccg cgcgacctcg aggtaatacc gggccgcatg ccggaacgat
      721 gcccgaacga ggcgctcgag ggcgtccgga tccgtggccg cgcgcctggc cgctgacggt
      781 ccgcgcccgc tcgccgcgag cccctcgcag acacgagcga ggttcgcgcg ggcctgcgcg
      841 cgccgcgccg gggcgacccg gtaccacagc tcgcccgcgg actcggcggc ggcgccgagg
      901 ggcgcctcag gcaggcgggc ggcgacggcc gccgcggcca tcacgagcga gccacggagc
      961 ccggggcgtc cgccggcctc gctcgacgcg gcttcgggag cggatccggt ggccgccgtg
     1021 ccgctcacgg cgaggcgacc agcgcggtcg cgcgagcctc gaggtcccgg gtctcggccg
     1081 gatcgtctgg ccacatcggc ttgaagctgt accactgctc gggcgcgagg gcgatgatcg
     1141 actcgagcgc gtcggcgatg cgctgcgtcg cgcgctggat gtcagcgggg tccgaagagg
     1201 gcaccgtgaa cgtctcgtac ggctcgaccc gcacccgccc gtcgccgatc cgccggatgg
     1261 cgaggggggc gatcatcgcg ccactcttcg cggccaaggc cgcgggtccc gccggcagcg
     1321 tcgtccaggt cccgaacagg cgcacgggga tcccatcgct gcgatagccc cagtcgacga
     1381 gcagggcgag gatctccttg cgcttgagca cgctgtacag cccgcgcagg ttgcgccacg
     1441 cgatgatctt gacgccccaa tcctcgcgtt ggcggctgag cagctcgaac aactcggggt
     1501 aggacgagtc gtcggcgatc acgctgaccg gatagccccg gcccgcgagt gcggcgccga
     1561 ccgcctcgtt gctcccgacg tgcccggcca ccacgatcat cggcctgccc gacgctcgcc
     1621 agtcggcgat gagtcggtcc acgccggcgt cctcgacccc cgcgaccagc tccgcctgcg
     1681 ggcgcgaggg gaggcgcatc agctcgacga cgtagcgcgc gtactcgcgg tacgcccgca
     1741 gcgcgagccg ccggacgcgc ggatgctccg gcggcagccc gagcacgtgg ccgaagttcg
     1801 cgttggagaa ccggcgcttc gccggccaga gcaggtagct gccctgggcc gcggtgccga
     1861 tcacggcctc cgagagtcgg ggtggcacat gcgcgaggac ggccgccgtg gccttgtagc
     1921 cgacgatggc cgtccgttcg acgatgcggg gagaggaggt ctgggggcgc cgatggggcg
     1981 cctccagtgg tcgcccctgg ctcaccgggt ggcggcggcc ttcgccttcg ccaccccggc
     2041 cagcgccttg gcgtccttgc gggacacctc gttgccgacc agcgtctcgt tgtcctcgcc
     2101 gcggatgaac gcctcgacgc gctgatgcgc gatgtcgtcg tggatctgga gcggggggct
     2161 cttcatgaag tagctcgacg gggcgacgag cgtgcccttg aggccgcgat cgaggccgat
     2221 ccgcaggcag cggatcgcgt cgatgatcac gccggcactg ttcgggctgt cccagacctc
     2281 gagcttgagc tcggcgttga gcgggacgtc accgaaggtc gtgccctcga tccggatgtg
     2341 acaccacttg cggtccttga gccacggcac gtagtccgac gggccgacgt ggatgtcctc
     2401 gtccgcgatc tcgtagtcga gcatcgaggt gacggcgttg gtcttgctga tcttcttgga
     2461 ctcgaggcgc tcgcgctcga gcatgttcag gaagtccgtg ttgccgccga agttcagctg
     2521 gtaggtgcgg tcgatccgca cgccgcggtc catgaacagg cgggtcagga cgcggtgggt
     2581 gatcgtcgcg ccgacctggc tcttgatgtc gtcgccgatg accgggaggc cctcctccgc
     2641 gaaccggcgc tgccagtagg gctcgcggcc gatgaacacc gggatcgcgt tgacgaagcc
     2701 gacgccggcc ttcagggcct gctcgacgta ccacttcgtg gcttcctcgc tcccgaccgg
     2761 gaggtaggag accatgacgt cgacctggcg ctccttcagg atcccgacga cgtcggccgt
     2821 ggggccaggc gccttctcga tgacctgcga gaggtacttg cccaggccgt cgtgggtcat
     2881 gccgcgatcg accttgacgc cgaggtgggg cacgtccatg aacttgaacg tgttgttctg
     2941 gcccgagtag atcgcctcgg aaaggtcctt gccgaccttc tccttgtcga cgtcgaacgc
     3001 cgccacgaac tccacatcgc tgatgtggta gtcgcccagc tggacgtgca tcaggccggg
     3061 cacgaagtcg ccggccttcg cgttctcgta gtagtagcgg ccctgcacga ggctgctcgc
     3121 gcagttgccg acgccgacga tggccactcg gaccttgtcg tcgcgggccc cgttgcgcgc
     3181 cgctccgttc gctctgccgt tgacggtgct catacctgga aaaccctcct aatgcgaaag
     3241 atgcgaagtc tggttggctg
//
LOCUS       DBNHXO010000048         5234 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4774459, whole genome shotgun sequence.
ACCESSION   DBNHXO010000048 DBNHXO010000000
VERSION     DBNHXO010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4774459"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..228)
                     /locus_tag="AC162A_01075"
     CDS             complement(13..228)
                     /locus_tag="AC162A_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009455.1"
                     /translation="MLKRTVAGILWLLAFWSAWAVVSVAVVLPPIPGVLIGTAIGVFV
                     AIDPFHRIWESTAAPSAQPAALEADAA"
     gene            329..2044
                     /locus_tag="AC162A_01080"
     CDS             329..2044
                     /locus_tag="AC162A_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015110127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR5009456.1"
                     /translation="MRFTRRARGQGAVTAGLTQVRGYRPDWFRADLLAGITVTAYLVP
                     QCLAYADLAGVEPVTGLWVAVVAMVAYALLGTSRQLSVGPESATAVMVAAAVAPLAAG
                     DPARYAALAAGLALLVGLVCIVAFVLRLGFLADLLSQPILIGYMAGVALIMIGSQLAT
                     ITGVTLTSDGAIARTVELSGKAGDVQPAAVALGLGVTAFLLILQRVAPLAPGSLIAVV
                     GAAVIVYVFDLAARGIALVGPVPSSLPSIALPALGVGDVTALFGSAAAIAFVAYTDVA
                     LTGRAFAVRTGEVIDANREFLGLGVANVASGLTGGFALSASGSRTAIIDAVRAHSQVA
                     GLVAAASVGVVVLAVPGLISYIPRAALGGIVVFAALRLIDVAALRRLGRFRMSELALA
                     LAALFGVLVFDVLAGILIAVGLSVADLFVRIARPPASVLGQVPGLAGLHDIDDYPEAR
                     QIPGLLVFRYDAPLCFANAEDFRTRALDAVDAANEGPTPVEWFLLNAEAIVELDMTSA
                     AALRRLVSELAERDVEFAMARVKQDFRAQLARGGLIGQIDEARMYPTLPVALEAFARR
                     AEGAP"
     gene            complement(2041..2877)
                     /locus_tag="AC162A_01085"
     CDS             complement(2041..2877)
                     /locus_tag="AC162A_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012730.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0034702 - monoatomic ion channel complex
                     [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HIR5009457.1"
                     /translation="MSDDPRTDLKGTGYELFMLLLSLLSIANAVIVILTGPASVGHQV
                     AFTIEVAITPFFLSDFVYRLLSTRSKRRYLVRRWGWADLLAIVPFLRVFRLVRIYLVV
                     REGRRVGRERLAEDLFVSRASTTFLFTVFLVIAVVEFAGMAEYYVEQGVPNANIVSAG
                     DSIWWGLVTITTVGYGDQYPVGEGGRLVGTILLFAGIGLFSVLTGFIANFFLAPDLPR
                     RRARLAPGTPAAEMAALHELLAEQERQHAAVRAKLHDLERSMLSRTREGGANPARAVD
                     GS"
     gene            3054..4817
                     /locus_tag="AC162A_01090"
     CDS             3054..4817
                     /locus_tag="AC162A_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR5009458.1"
                     /translation="MAAVTERRTRTPAWILNGVLPLSGKRIPLDIAAGATLAALAIPE
                     TMGYASMAQMPVITGLYTILIPLFLFAVLGSSRHLVVGADSATAMTMAAGLTALGLVG
                     GSSEWIAMAGLSALMVGVVLILARLLKLGFLAYFLSRSVLIGFLTGVGIQVAMTQLAG
                     VLGVPKPAGGVLEQFVKTLQEIPQTNVATLAVSVAVWVIILGSDRINKRIPGALIAVV
                     LMIAISYLGLLPSSVTLLGAVQGGLPPIGLPQGVITVSNVLALLPIVLSCFLIILAQS
                     AATSRAYAMKYRDSFDENVDLIGLAAANFGAGVSGTWLVNGSPTKTEMVDGAGGRSQV
                     AQLTAGLVVLVVLLFLTPALAYMPNAVLAAVVMLIGIRLIDILGMRGIARVRTGEFVV
                     AAITAATVVLVGIEQAILLAIALSIVEHIDHSYHPFDRLLTSKPGGDYGFEEVATGAQ
                     ALPGLVIYRFGASLYYANGNRFSEEIVHLVEDADPPLRWLCVSATSIGDIDYSGAETI
                     EAVREEILGHGVTLVFADVDPKISGQLDAYELTDKIGKDHIFASLLEAIAAFGATAGS
                     AAPAVAPATTGDGPAPAPSTG"
     gene            complement(4839..>5234)
                     /locus_tag="AC162A_01095"
     CDS             complement(4839..>5234)
                     /locus_tag="AC162A_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR5009459.1"
                     /translation="VFVSAPFFIRSARTGLTSVPRELEDAARVDGASELYLFRRVTLP
                     IARAALAAGLVMTWARSLGEFGATIMFAGNVQGRTQTLPLVVYSEFNSGDLEASIAAA
                     AILVLAAFGVLIAVRVLHWGRVLDDRSLG"
BASE COUNT          732 a         1845 c         1837 g          820 t
ORIGIN      
        1 tgcggcatgg ggtcaggcgg cgtccgcctc cagcgcggcc ggctgggccg acggcgcggc
       61 tgtcgactcc cagatccgat ggaacgggtc gatggcgacg aatacgccga tggccgttcc
      121 gatcagaacg ccggggatcg ggggcaggac gaccgccacc gagacgacgg cccatgcgga
      181 ccagaaggcc aggagccaga ggatcccggc aaccgttcgc ttcaacatgg cgtgagcatc
      241 gtcgtgagcc cgtcggcgcg gcaatggctc attcgtactc gcccgggaac gcaaaccggc
      301 ccaccacggc gcgcctacca tcgcgcacat gcgcttcact cgccgggccc ggggtcaggg
      361 cgcggttacg gccggcctga cgcaggtgcg gggctaccgg ccggactggt tccgcgccga
      421 tctcctggcc gggatcacgg tgaccgccta cctcgtcccc cagtgcctcg cctacgccga
      481 cctcgcgggc gtcgagccgg tgaccggcct gtgggtcgcg gtcgtggcga tggtcgccta
      541 cgcgctcctc gggacatcgc gccagctgtc ggtcggcccc gagtccgcca ccgcggtcat
      601 ggtcgcggcc gcggtcgcgc cactggcggc gggcgatccc gcccggtacg cagcgctggc
      661 ggccggcctc gccctgctcg tcggcctggt gtgcatcgtg gccttcgtcc tgcggctggg
      721 gttcctcgcc gacctgctgt cacagccgat cctgatcggc tacatggcgg gggtcgcgtt
      781 gatcatgatc gggtcgcagc tggcgacgat cacgggcgtc acgctcacgt cggacggggc
      841 gattgcccgg acggtcgagc tgtcgggcaa ggccggcgac gtccagccgg ccgcggttgc
      901 cctggggctc ggcgtgacgg cgttcctgtt gatcctgcag cgcgtcgcgc cgctggcgcc
      961 gggctcactg atcgcggtcg tgggcgcggc ggtcatcgtc tacgtcttcg accttgccgc
     1021 tcgcggcatc gcgctggtcg gtccagtgcc gagcagcctg ccgtcgattg cgctgcccgc
     1081 cctcggcgtt ggcgacgtca ccgcgctgtt cgggtcggcg gccgcgatcg cgttcgtcgc
     1141 gtacacggac gtcgcgctga ccgggcgcgc gttcgcggtt cgcaccggcg aggtgatcga
     1201 tgccaaccgc gagttcctcg ggctcggggt ggccaacgtg gcctctgggc tgaccggggg
     1261 atttgcgctc agcgcctcgg ggtcgcggac cgcgatcatc gacgctgtcc gcgcccacag
     1321 ccaggtcgcc ggcctggtgg ccgcggcgtc ggtcggcgtc gtggtgctgg ccgtgcccgg
     1381 gctgatctca tacatcccgc gggccgcgct gggcgggatc gtcgtgttcg ccgcactacg
     1441 cctgatcgac gtcgcggcgc tccgccgcct cgggcggttc cggatgtcgg agcttgcgct
     1501 tgcgctggcg gcgctgttcg gcgtgctggt gttcgacgtg ctggccggga tcctgatcgc
     1561 ggtcggccta tcggtcgccg acctgttcgt ccggatcgcg cggccgccgg cctcggtcct
     1621 cggccaggtg ccggggctcg ccggcctcca cgacatcgac gactacccag aggcacggca
     1681 gatcccgggc ctgctcgtgt tccgctacga cgcgccgctg tgcttcgcca acgccgagga
     1741 cttccggacc cgggcgctcg atgcggtcga cgccgccaac gagggtccga ctccggtcga
     1801 gtggttcctg ctcaacgcgg aggcgatcgt ggagctggac atgacgtcgg ccgcggccct
     1861 gcgccgcctc gtgagcgagc tggcggagcg cgacgtcgaa ttcgcgatgg cacgcgtaaa
     1921 gcaggacttc cgagcccagc tcgctcgggg cgggcttatc gggcagatcg atgaggcgcg
     1981 gatgtacccc accctgccgg tcgccctcga ggcctttgcg cgacgagcgg aaggggcgcc
     2041 ctagctcccg tccacggcgc gtgccggatt ggcgccccct tcgcgcgtgc gcgagagcat
     2101 cgacctctcg aggtcgtgca gcttcgctcg caccgcagcg tgctggcgct cctgctcggc
     2161 gaggagctcg tggagtgccg ccatctccgc ggccggcgtt ccgggcgcaa ggcgcgcccg
     2221 gcgacgcggc aggtccggcg cgaggaagaa gttcgcgatg aaaccggtca gcacggagaa
     2281 cagcccgatt ccggcgaaca acaggatggt cccgaccagg cggccgccct cgccgaccgg
     2341 atactggtcg ccatacccca cggtggtgat cgtcaccagg ccccaccaga tcgaatcacc
     2401 cgccgacacg atgttcgcgt tcggcacgcc ctgctcgacg tagtactccg ccatgccggc
     2461 gaactcgacg accgcgatca cgaggaacac cgtgaacagg aacgtggtcg acgcccggct
     2521 cacgaagagg tcctcggcca ggcgctcccg ccccacccgc cggccttcgc gcacgacgag
     2581 gtagatcctg acgagccgga acacccgcag gaacggcacg attgcgagga ggtccgccca
     2641 cccccagcgc cggaccaagt atcggcgctt cgagcgggtg ctcaggagcc ggtacacgaa
     2701 gtccgagagg aagaagggcg tgatcgcaac ctcgatggtg aacgcgacct ggtgccccac
     2761 cgaggccggg ccggtcagga tcacgatcac ggcattcgcg atcgagagca gcgacagcag
     2821 gagcatgaac agctcgtagc cggtgccctt gaggtcggtt cgcggatcat cggacatcgg
     2881 gcgtctccag ggtgcggggc ctcgattgtg cgacggcggc gggcacccgt gcggggcctc
     2941 gcgtcgcctg ccgagacccc caacgattga tgacgcggtc gccggagcag cgaccgtatg
     3001 ctccgcgctt cggacgtgac ggtccgaggg ggacgagggc ggaggaggag gccatggcgg
     3061 cagtgacgga gcgccggacg cggacgccgg cgtggattct caacggcgtc ctgccgctca
     3121 gcggcaagcg gatcccactc gatatcgccg ccggtgcaac gctcgcggcg ctggcgatcc
     3181 ccgagacgat gggctacgcc tcgatggcgc agatgcccgt catcacgggc ctgtacacga
     3241 tcctcattcc gctgttcctg ttcgcggtcc tgggctcgtc gcgacacctc gtcgtcggcg
     3301 cggactccgc gacggcgatg accatggcgg ccggcctgac ggcgctcggg ctggtcgggg
     3361 gatcttcgga gtggatcgcg atggccggcc tgtcggccct catggtcggc gtcgtcctca
     3421 tccttgcccg gctcctcaag ctgggcttcc tcgcctactt cctgtcgcgc agcgtgctca
     3481 tcggtttcct gaccggcgtc ggcatccagg tcgccatgac ccagcttgcg ggcgtgctcg
     3541 gggttccgaa gcccgcgggc ggcgtcctcg agcagttcgt caagacgctc caggagatcc
     3601 cgcagacaaa cgtggcgacg ctggcggtct cggtcgcggt ctgggtcatc atcctgggct
     3661 ccgaccgcat caacaagcgg atcccggggg cgttgatcgc ggtggtcctc atgatcgcga
     3721 tcagctacct cggcctgctg ccgagctcgg tcaccctgct cggcgcggtc cagggtggac
     3781 tccccccgat cggcctcccg cagggcgtga tcaccgtcag caacgtgctc gccctgctgc
     3841 ccatcgtctt gtcctgcttc ctgatcatcc tcgcccagag cgccgcgaca tcccgcgcgt
     3901 atgcgatgaa gtaccgcgac agcttcgacg agaacgtcga cctcatcggc ctcgcggcgg
     3961 cgaacttcgg cgccggcgtc tcggggacct ggctcgtgaa cggcagcccg accaagaccg
     4021 agatggtcga cggagccggc ggacggtcgc aggtcgccca gctgaccgcc ggcctcgtgg
     4081 tcctcgtcgt cctgttgttc ctgacgccgg cgctcgccta catgcccaac gcggtcctcg
     4141 ccgccgttgt catgctcatc ggcatccggc tgatcgacat cctcggcatg cgcggcatcg
     4201 cgcgggtccg gaccggtgag ttcgtggtcg ccgccatcac ggccgcgacc gtggtcctgg
     4261 taggaatcga gcaggcgatc ctgctcgcga tcgcgctgtc gatcgtcgag cacatcgacc
     4321 atagctacca tccgttcgac cgcctactga cgtccaagcc gggcggcgac tacgggttcg
     4381 aggaggttgc caccggtgcc caggccctgc cgggcctcgt gatctaccgc ttcggtgcca
     4441 gcctctacta cgcgaacggc aaccgcttct cggaggagat cgtccacctc gtcgaggacg
     4501 ccgacccgcc gctccgctgg ctgtgcgtgt cggcgacgtc gatcggcgac atcgactact
     4561 cgggcgcgga gacgatcgag gcggtgcgcg aggagatcct gggccacggg gtcaccctgg
     4621 tgttcgccga cgtcgacccc aagatcagcg gccagctcga cgcctacgag ctcaccgaca
     4681 agattggcaa ggaccacatc ttcgcgtcgc tgctcgaagc catcgcggcg ttcggtgcaa
     4741 ccgctggttc agccgcgcca gcggtcgcgc cggcgacgac cggcgatggc cccgcgccgg
     4801 ccccttcgac cggctgacgc cagaccgacg gccgcagctc agccgaggct gcggtcgtcg
     4861 aggacgcggc cccagtgcag gacacggacg gcgatcaaca ccccgaaggc ggctagcacc
     4921 aggatcgcgg cggcggcgat ggacgcttcc agatcgccgc tgttgaactc ggagtacacg
     4981 acgagcggca acgtctgggt cctgccctgg acgttgccgg cgaacatgat cgtggcgccg
     5041 aactcgccca gcgacctggc ccaggtcatg accaggccgg cggccagggc cgcgcgagcg
     5101 atcgggagtg tgaccctccg gaacaggtag agctcgctgg cgccgtcgac gcgggccgcg
     5161 tcctcgagct cgcggggcac gctggtcagg ccggtgcgcg cggatcggat gaagaacggg
     5221 gccgagacga agac
//
LOCUS       DBNHXO010000049         1698 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1203868, whole genome shotgun sequence.
ACCESSION   DBNHXO010000049 DBNHXO010000000
VERSION     DBNHXO010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1203868"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1118)
                     /gene="cydB"
                     /locus_tag="AC162A_01100"
     CDS             complement(102..1118)
                     /gene="cydB"
                     /locus_tag="AC162A_01100"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HIR5009460.1"
                     /translation="MLEELWFVLFVLIVAAYLILDGFDMGVGMLMIPMGKNDDERRTM
                     LNSIGPIWDGNEVWLVIAGGVLFGVFPLVYASLFSGFYLAFMLVLFVMILRTVAMEFR
                     SKEPSPRWRSFWDMVFALASTGLAFLLGVAFGDVLSGVPMDADGNMSVSLIGLLKPVP
                     LLVGATTIAMFAMHGSLYLVQKTEGELQARIRRAVPRLIAAFFVLNTLVVVAMVLFRQ
                     QITERYVDDIWPIIFPAAALVAILMAYRFVRQGREFAAFLASGATIALLVISGALGMF
                     PNLIISTTDPAYNLTIYNAAAAENTLVVCLIVAVIGLPFILAYTAGVYYFFRGKSTVD
                     SHGY"
     gene            complement(1118..>1698)
                     /locus_tag="AC162A_01105"
     CDS             complement(1118..>1698)
                     /locus_tag="AC162A_01105"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIR5009461.1"
                     /translation="QTTTGISIPCLLSVLAYFNPQATVQGINSFAPDPTPPINLVFQV
                     YHFMFGLGTLFMPIGLLAGLLYWRKQRLFTARWMLWILVVTVFFVEAVIIAGWWTAEI
                     GRQPWVVYNVLKTADGVSPTLAGIDVAVSLVMFIVLYAILLVLFLFLLNRAIQRGPEP
                     LEEVETVDRATLPDTFREIFTRRDRAGSSGER"
BASE COUNT          347 a          530 c          582 g          239 t
ORIGIN      
        1 gaatcagccg cgcgcgcgga tccccggcga gcagcctgga ctcgggaccg ggcgggcgac
       61 gtgaacggac cggcctcgcc cgctcgtcgc gggtgagggt ctcagtagcc gtgcgagtcg
      121 accgtggact tgcctcggaa gaagtagtac acgccggcgg tgtacgcgag gatgaacgga
      181 agtccgatca cggcgacgat caggcacacc acgagcgtgt tctcggccgc ggcggcgttg
      241 tagatcgtca ggttgtaggc gggatcggtg gtcgagatga tcaggttcgg gaacatcccg
      301 agggcgcccg aaatgacgag cagggcgatc gtcgcgcccg aggcgaggaa tgcggcgaac
      361 tcgcgtccct ggcgtacgaa gcggtacgcc atcaggatgg ccaccagggc cgcggccggg
      421 aagatgatgg gccagatgtc gtccacgtag cgttccgtga tctgctggcg gaagagcacc
      481 atcgcgacca cgaccagcgt gttgaggacg aagaaggccg cgatcagccg cggcaccgcc
      541 cgccggatcc gggcctgcag ctcgccctcg gtcttctgga cgaggtacag gctcccgtgc
      601 atcgcgaaca tcgcgatggt cgtggcgccg accagcagcg ggaccggctt gagcagcccg
      661 atcagggaga ccgacatgtt gccgtctgcg tccatcggca cccccgacag cacgtcgccg
      721 aacgccacgc ccagcaggaa cgccagcccc gtcgaggcga gcgcgaacac catgtcccag
      781 aacgaacgcc agcgcggcga gggctccttg ctacggaact ccatcgcgac ggtccgcagg
      841 atcatcacga acagcacgag catgaatgcc aggtagaagc cggagaacag tgaggcgtag
      901 acgagcggga acacgccgaa cagcacgccg cctgcgatga ccagccagac ctcgttgccg
      961 tcccagatgg ggccgatgct gttgagcatc gtccggcgct cgtcatcgtt cttccccatc
     1021 gggatcatca gcatcccgac gcccatgtcg aagccgtcga ggatcaggta ggcggccacg
     1081 atcaggacga acaggacgaa ccagagctct tcgagcatca gcgttctccg ctcgagccgg
     1141 cacgatcccg gcgcgtgaag atctcacgga acgtgtcggg cagggtggca cggtcgacgg
     1201 tctcgacctc ctcgagtggt tcgggaccgc gctggattgc ccggttgagg aggaacagga
     1261 acaggacgag caggatcgcg tacaggacga tgaacatgac gagggagacg gcgacgtcga
     1321 tccccgccag cgtcggcgat accccgtcag ccgttttgag gacgttgtag acgacccatg
     1381 gctggcgtcc gatctccgcg gtccaccagc cggcgatgat cacggcttcc acgaagaaca
     1441 cggtcaccac gaggatccag agcatccagc gggccgtgaa caggcgctgc ttccgccagt
     1501 acagcaaccc ggcgagcagg ccgatcggca tgaacaacgt ccccaagccg aacatgaagt
     1561 gatagacctg aaacaccagg ttgatcggcg gggtcgggtc gggtgcgaac gagttgatgc
     1621 cctgaaccgt ggcctgcggg ttgaagtagg cgagcacgct gagcaggcac gggatcgaga
     1681 tccccgtcgt ggtctggg
//
LOCUS       DBNHXO010000050         9442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4179972, whole genome shotgun sequence.
ACCESSION   DBNHXO010000050 DBNHXO010000000
VERSION     DBNHXO010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 9442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9442
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4179972"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..978)
                     /locus_tag="AC162A_01110"
     CDS             complement(151..978)
                     /locus_tag="AC162A_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009462.1"
                     /translation="MHRWSHRQPDQADPAQETKPAPRVGAPGSVEPVLQLQAVVGNAA
                     VTSLLRAGGGAPTLGIQRLWDTDEEEDVPSDDGPSEDNGGGAPTPEESPEDQPAEETT
                     DPYAAVETEVTSTLDPGTVLDTLAPLETPERQLGDFEVPETGETAQALRIQRDNTPEA
                     PGPTREGKPGDIIKAFKPYLEPALKWLEQNVLDALKKIKAGEAVITVIVAAPIIIGPL
                     TQPGPRRLALDQLDGTDVTFGVIPNLKIKPQITDGELRGGTVTYDLAPALRKWGIPF"
     gene            1098..1946
                     /locus_tag="AC162A_01115"
     CDS             1098..1946
                     /locus_tag="AC162A_01115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR5009463.1"
                     /translation="MNVFVTALVLAAALLLAGCQPTGDTGSSPGVSAAPGRSAAAAPS
                     TAASNALQLVALGDSETTGSGDRTGRGWVEYYAERIREGTGREVNVTNLARNGTRSLD
                     LVMAVQQAADIRNAIAAADIVVLGIGGADLNAGDALLEAGSCGGTDCYDASIAAYGAN
                     IDAIAAEIVNLRGGQPTALRAITQPNALTGAESVIPPFLRSISTEVGVHVARGFNAAT
                     CAAMAAHDGACVDVLTAINGPDGTGDGYATGLMNIADCCYPSERGHRLIADLLYKTGL
                     EPLANP"
     gene            complement(1952..3109)
                     /locus_tag="AC162A_01120"
     CDS             complement(1952..3109)
                     /locus_tag="AC162A_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014728.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5009464.1"
                     /translation="MTDPDRDAFDVRPTGDEMLARVRAEELDGRGRLRIYLGMAPGVG
                     KTFRMLEEGHRRLERGTDVVVGFAEAHGRPHTLELLDGLEVVPRERIEYRGIVVEEMD
                     TVAILERRPTVALIDELAHTNVPGSARGKRWEDVEIVRDAGIHVVTTMNVQHVASVSD
                     AVATITGAPVNERLPDDVLMTADEIELVDMSPHALRQRMKHGNVYPPDRTQVALERFF
                     TESNLTALRELSLRLVARRVEGQLEDTIAGQQLPLVTDRVLVLVDGSPATPRAVRRAA
                     MLAGTLHATLVAVVVETPDLARESFDRRRDIQEALDDAVDLGAEVVTIEAKDLVSGLE
                     EVSRSRRATHVVLPFRAQSGVARLRDRPVVDRLIERLPDVELHVVGPPAKR"
     gene            complement(3106..3705)
                     /gene="kdpC"
                     /locus_tag="AC162A_01125"
     CDS             complement(3106..3705)
                     /gene="kdpC"
                     /locus_tag="AC162A_01125"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HIR5009465.1"
                     /translation="MTTFLRRVLWPAFALLVAMTLITGVAYPAVVTAVAQVAFPHQAN
                     GSMLVAKDGRTVGSSLIGQAFSGDTYFWGRLSAAGATDDNPLGYDASSSAGSNLGPTN
                     QDLLDRVEQSVADYQAANPGVPIPVDLITTSASGLDPHISPAAAEYQVARVATARGLS
                     EADLRAVVARHTEQPLFGIFGQPRVHVLELNLDLDGLLQ"
     gene            complement(3716..5872)
                     /gene="kdpB"
                     /locus_tag="AC162A_01130"
     CDS             complement(3716..5872)
                     /gene="kdpB"
                     /locus_tag="AC162A_01130"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HIR5009466.1"
                     /translation="MSVDLGRGTRRFQRFVTLGGAKPSGEPPRPRGILDPELLRSALP
                     HAVRKLDPRLLIRNPVMFVVEITAALVTLIWIASVTGIQPDPGTSGSGFQLQIGVWLW
                     FTVLFATYAEAVAEARGRAQAATLRRTRSETTAHRRRDDGTLEAVGSSELRKGDVIVV
                     EEGEVIPGDGDVIEGVGFVNEAAITGESAPVLKEPGTDIRSSVTGGTTLISDRLVIRV
                     TTDPGETFLDRMIALVEGAKRQRTPNEIALAILLAGLTIVFLMATVTLRPFGEYAGTT
                     VETVALVALLVCLIPTTIGGLLSAIGIAGMDRVARFNVLAMSGRAVEASGDVDVILLD
                     KTGTITYGNRLASSITPAPGVSEGDVLQAALVSSIRDETPEGRSVVDLARKRLAELGL
                     PAGAGDDAAFTALGATISEEIPFKAETRTSGVRLGGGATILKGAVDAITAGLTDAVPP
                     EVIAEADRIADAGATPLAIRDGGRILGMIELKDTVKEGLVERFAEFRKMGIRTVMITG
                     DNPRTAATIAREAGVDDFIAQATPEAKIAFIRQQQADGHLVAMTGDGTNDAPALAQSD
                     VGLAMNSGTSAAKEAANMVDLDSDPTKLLEVIEIGKQLLITRGSITTFSIANDIAKYF
                     AILPALFVSVYPDLGALNVMGLATPQSAILSAVIFNALIIIALIPLALRGVAFRAAPA
                     GALLQRNLLIYGVGGIIAPFIGIKAIDVVVSALNLA"
     gene            complement(5898..7610)
                     /gene="kdpA"
                     /locus_tag="AC162A_01135"
     CDS             complement(5898..7610)
                     /gene="kdpA"
                     /locus_tag="AC162A_01135"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HIR5009467.1"
                     /translation="MGSGDLFVLFTLAVALLVATPLLGAYIHRVMEGERVFLSPILRP
                     VERAVYRVCGVDESTEQGWRGYTIAVLVLAAVAIAAGYIVLRLQDVLPLNQGGIPAMS
                     PDLAFNTSVSFETNTNWQNYSGETGATYLTQAGVLAVRNFTSAATGLAIAVALFRGLT
                     RRSATTIGNYWVDLTRGILYLLLPISIIGALVLVWQGAPQTWNPAQAVTTLEGAVQHI
                     AIGPVASQEWIKELGNNGGGFMNANSAHPFENPTALTNWLEMFAILLFPFALTYAFGR
                     YAGDQRQGWTIFAAMAIVLSIGAVGAMHVEYVGNPLFPAAADQAGLGNMEGKEVRFGS
                     ALGGLFMAVTTGTSTGAINSWHDSAQPIAGLVPLFNMELGEVTPGGIGAGMYGMLIIG
                     AILAVFIAGLMVGRTPEYLGKKVEAFEIKMAMLVALVLGASILGFTALAAATPEGQAG
                     PLNTGPHGFSEILYAFSSQTGNNGSAFAGLTGNTPFYNVTGAIAMLIGRYGMIVPILA
                     LAGSMAAKRRVAPSLGTFPTTGPLWTVLLIGVVIIVGALTFFPALSLGPIVEQLIQNA
                     GKVS"
     gene            complement(7614..7703)
                     /gene="kdpF"
                     /locus_tag="AC162A_01140"
     CDS             complement(7614..7703)
                     /gene="kdpF"
                     /locus_tag="AC162A_01140"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HIR5009468.1"
                     /translation="MNVLEVLTALIAAFLFVYLLWALLRPEDF"
     gene            complement(7786..8178)
                     /locus_tag="AC162A_01145"
     CDS             complement(7786..8178)
                     /locus_tag="AC162A_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5009469.1"
                     /translation="MPERPRQILIVEDDESLRRIVVRHLRSLGFRVAEADSAEAAVEA
                     LDRGLRPDVVILDLNLPGDTGWDLLRGAALDAAGRPPVVITSATTVSPKWLDEFGVAG
                     YLPKPFPLETLVATVERLTNPEATQQQP"
     gene            complement(8171..8425)
                     /locus_tag="AC162A_01150"
     CDS             complement(8171..8425)
                     /locus_tag="AC162A_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009470.1"
                     /translation="MDASARHVPSAGRTLEQELLLVREAIDMVAGGGAPRVVVGGLQF
                     GEALIEPARELAGAAGVLVVPLWMPDDAGADIAIERMPDA"
     gene            complement(8483..9229)
                     /locus_tag="AC162A_01155"
     CDS             complement(8483..9229)
                     /locus_tag="AC162A_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5009471.1"
                     /translation="MTSPRPAAGGPTVLVVEDDEETRAALRRELAVRGYRTVEAADGR
                     AALRAWESRRPDLVLLDLGLPDMDGLDIVRQIRREAATPIVILSGRYEEREKVEALER
                     GADDYVTKPFGVDELNARLQVALRHAAGPVADVAGTIRAGPLEFDAGRHAARVGDVPL
                     DLTPREFEILRVLLTNAGRLVTKGRLLRAVWGEAYQGEDAYIYVHVSQLRRKIAAADP
                     GGALRDLIVTEPGVGYRVRELDRPTALDRP"
     gene            complement(9226..>9442)
                     /locus_tag="AC162A_01160"
     CDS             complement(9226..>9442)
                     /locus_tag="AC162A_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5009472.1"
                     /translation="TLPLLFDKFYRVPGTPGGSRSGSGIGLAVVRGLVEAMDGRVDAR
                     RSELGGLAIDIDLPAATAPEPAPAVPG"
BASE COUNT         1406 a         3199 c         3447 g         1390 t
ORIGIN      
        1 tcccgccgcc gacgacgacg acgcgagcgt ggctcgggag ctgcgaggcg ggcaggacac
       61 cggcgacgtc gagatcgcgg ttggcgagat gttcgggcac gggcatggcc gccgcatcgt
      121 agaacgaccg tggcccgtcg ggagcggaga tcagaagggg atgccccact tgcggagcgc
      181 gggggcgagg tcgtaggtga cggtgccgcc gcggagctca ccgtcggtga tctggggctt
      241 gatcttgagg ttcgggatca ccccgaaggt gacgtccgtc ccatcgagct gatcgagcgc
      301 caggcgacgc ggacctggct gcgtcagggg gccgatgatg atcggcgccg cgacgattac
      361 cgtgatcacg gcctcgcccg ccttgatctt cttgagcgcg tcgaggacgt tctgctcgag
      421 ccacttgagc gcgggctcga gatagggctt gaaggccttg atgatgtccc ccggcttgcc
      481 ctcgcgcgtc ggacccggtg cctcgggcgt gttgtcgcgc tggatgcgga gcgcctgggc
      541 ggtctcgccg gtctcgggca cctcgaagtc gccgagctgt cgttccggag tctcgagcgg
      601 cgccagggtg tcgagcacgg tgcccgggtc gagcgtcgag gtgacctcgg tctcgaccgc
      661 ggcgtacggg tccgtcgtct cctcggccgg ctgatcctcg ggcgattctt cgggcgtcgg
      721 cgcgccaccg ccgttgtcct cggagggccc gtcgtcagaa ggcacgtcct cctcctcgtc
      781 ggtgtcccac aggcgctgga tgcccagggt cggcgccccg ccgcccgcgc ggagcaggct
      841 cgttacggcg gcgttgccca cgacggcctg cagctgcagg acgggctcga cgctgccggg
      901 cgcaccaacg cgcggcgcag gcttcgtttc ctgcgccgga tcggcttgat caggctgtcg
      961 gtgtgaccag cggtgcatcg atgaacgcgc ctccaagcgc agctcgagga tgcgtggcga
     1021 ccaaacgcga tggcagcgcc gttgaggcaa gcaacgtcgc ccaaccgggc aaacccagcc
     1081 acgcgtagga tcgccgcatg aacgtgttcg tcactgcgct tgttctggcg gccgccctct
     1141 tgttggccgg ttgccagccg accggggaca cggggtcaag cccgggcgtc tcggcggcgc
     1201 ctggtcgatc tgcagcagct gcaccgtcga ccgccgcgtc gaacgccctc cagctcgtcg
     1261 cgctcgggga ttccgagaca accggatcgg gcgaccgcac ggggaggggc tgggtcgagt
     1321 actacgcgga gcgcatccgt gagggcaccg gtcgcgaggt gaacgtcacg aaccttgctc
     1381 ggaacggcac ccgaagcctc gacctcgtga tggcggtgca gcaggccgcg gacatccgca
     1441 atgcgatcgc ggccgccgac atcgtggtcc tcgggatcgg cggcgcggac ctgaacgcag
     1501 gtgacgccct cctcgaggcc ggctcgtgcg gcgggaccga ctgctacgac gcatcgatcg
     1561 ccgcctacgg cgcgaacatc gacgccatcg cggccgagat cgtcaacctc cgcggcggcc
     1621 agccgaccgc cctgcgcgcg atcacccagc ccaacgccct tacgggtgcc gagtcggtga
     1681 tcccgccgtt cctgcgatcg atctcgaccg aggtcggtgt ccacgtcgcg cgcggtttca
     1741 acgcggccac ttgcgcggcg atggccgcgc acgatggcgc ctgtgtcgac gtccttacgg
     1801 caatcaacgg gcccgacggc accggcgacg ggtacgcaac gggcctgatg aacatcgccg
     1861 actgctgtta ccccagcgag cgtggccacc ggctgatcgc ggacctgctg tacaagacgg
     1921 gcctcgaacc gctggcgaac ccgtaggccg gtcagcgctt cgcgggcggg ccgacgacgt
     1981 gcagctcgac gtcgggcagg cgctcaatga ggcgatcgac gaccgggcga tcccggagcc
     2041 gggccacgcc ggactgggcc cgaaagggca ggacgacgtg ggtggcccgc cgcgagcggc
     2101 tgacctcctc gaggccgctt acgaggtcct tcgcctcgat ggtgacgacc tcggcgccga
     2161 gatccacggc atcgtcgagc gcctcctgga tatcgcggcg tcgatcgaac gactcgcgtg
     2221 cgaggtccgg ggtctcgacg accaccgcca cgagggtcgc gtggagcgtt cccgcgagca
     2281 tcgccgcgcg gcgaacggca cgtggcgtcg cggggctgcc gtcgacgagg acgaggacgc
     2341 gatcggtgac gagcggcagc tgctggcccg cgatcgtgtc ctcgagctgg ccctccacgc
     2401 gccgggcgac caggcgcagg ctgagctcgc gcagcgcggt gaggttcgac tcggtgaaga
     2461 accgctcgag cgcgacctgt gtccggtccg gcgggtagac gttgccgtgc ttcatgcgct
     2521 gtcggagcgc atgggggctc atgtcgacca gctcgatctc gtccgccgtc atgaggacgt
     2581 cgtcggggag gcgctcgttg accggcgcgc cggtgatcgt ggcgacggca tcggacacgc
     2641 tcgcgacgtg ctggacattc atggtggtca cgacgtggat ccccgcgtcg cggacgatct
     2701 ccacgtcttc ccagcgcttg ccgcgtgccg agccgggcac gttggtgtgg gcgagctcat
     2761 cgatgagagc gacggtcggc cggcgttcga gaatggcgac cgtgtccatc tcctcgacca
     2821 cgatgccgcg atactcgatc cgctcgcgcg ggacgacctc gaggccgtcg aggagctcga
     2881 gggtatgcgg ccgcccgtgc gcctcggcaa agccgaccac gacgtccgtg cctcgttcga
     2941 ggcgccgatg gccttcctcg agcatccgga acgtcttgcc gacgccgggc gccatgccca
     3001 ggtagatgcg cagccgtccc cggccatcca gttcctcggc gcgaacgcgc gcgagcatct
     3061 cgtcgcccgt cgggcggacg tcgaacgcgt cgcggtcggg atctgtcact gcaggagccc
     3121 gtcgaggtcc aggttgagct cgagcacgtg gacgcgcggc tggccgaaga tcccgaacag
     3181 gggctgttcg gtgtggcggg cgacgacggc ccggaggtcg gcctcggaca gcccacgcgc
     3241 cgtggcgacc cgcgcgacct ggtactcggc cgccgccggg ctgatgtggg gatccagccc
     3301 ggacgccgac gtcgtgatca ggtccaccgg gatcggcacg ccggggttgg cggcctggta
     3361 gtcggcgacg ctctgctcga cccggtcgag caggtcctgg ttcgtcggcc ccaggttcga
     3421 gccggccgag ctgctggcgt cgtagccaag cgggttgtcg tcggtcgccc cggcggcgga
     3481 gaggcggccc cagaagtagg tatcgccgga gaacgcctgc ccgatcaggc tcgagccgac
     3541 ggtccggccg tccttggcga cgagcatcga cccgttggcc tggtgcggga aggcgacctg
     3601 ggcgaccgcg gtcaccacgg cagggtaggc gacgccggtg atcagggtca tggcgacgag
     3661 gagggcgaac gccggccaca ggacgcggcg caggaaggta gtcatcaggg agtccttacg
     3721 cgagattcag ggccgagacg acgacgtcga tcgccttgat cccgatgaac ggggcgatga
     3781 tgccgccgac gccatagatc agcaggttgc gctgcagcag cgcgccggcc ggcgccgcgc
     3841 ggaaggcgac gccccggagg gcgagcggga tcaacgcgat gatgatcagc gcgttgaaga
     3901 tcaccgcgga caggatcgcg ctctgcggtg tggcgaggcc catgacgttg agggccccga
     3961 ggtccgggta gacgctcacg aacagcgcgg gcaggatcgc gaagtacttc gcgatgtcgt
     4021 tggcgatcga gaaggtcgtg atcgatccgc gcgtgatgag cagttgcttg ccgatctcga
     4081 tcacctcgag cagcttcgtc gggtcggagt cgaggtcgac catgttggcc gcctccttgg
     4141 cggccgacgt gccgctgttc atcgccagcc cgacatccga ctgggcgagc gccggcgcat
     4201 cgttggtgcc atcgccggtc atggcgacga ggtgaccatc cgcctgctgc tggcgaatga
     4261 acgcgatctt cgcctcgggc gtggcctgcg cgatgaagtc gtcgacgccg gcctcgcgcg
     4321 cgatcgtcgc ggccgtcctg gggttgtcgc cggtgatcat caccgtccgg atgcccatct
     4381 tgcggaactc ggcaaaccgc tcgacgaggc cttccttgac ggtgtccttc agttcgatca
     4441 tgccgaggat gcgcccgcca tcgcggattg cgaggggagt cgccccggca tcggcgatcc
     4501 ggtccgcctc ggcgatcacc tccgggggga ccgcgtcggt gagcccggcc gtgatcgcgt
     4561 cgacggcccc cttgaggatc gtcgcgccgc ccccgagccg gaccccgctc gtgcgcgtct
     4621 ccgccttgaa cgggatctcc tcggagatcg tcgcgccgag ggccgtgaac gcggcgtcgt
     4681 cgcccgcgcc ggccggcagg ccaagctcgg cgagccgctt ccgggcgagg tccacgaccg
     4741 accggccctc gggcgtctcg tcgcggatcg aggacaccag ggctgcctgg aggacatcgc
     4801 cctcggacac gccgggtgcg ggcgtgatcg acgacgccag ccggttgccg taggtgatcg
     4861 tgccggtctt gtcgagcagg atgacgtcga cgtccccgga tgcctcgacc gctcggccgc
     4921 tcatggcaag cacgttgaag cgcgcgacgc ggtccatgcc ggcgatgccg atggcggaca
     4981 gcaggccgcc gatggtcgtc gggatgaggc acacgaggag cgcgaccagg gcgacggtct
     5041 cgaccgtggt cccggcgtac tccccgaacg gccgcagcgt cacggtggcc atcaggaaga
     5101 cgatggtcag gccggcgagc aggatcgcga gcgcgatctc gtttggcgtc cgctgccgct
     5161 tcgcgccctc gacgagcgcg atcatccggt cgaggaacgt ctcgccgggg tcagtggtga
     5221 cgcggatgac caggcggtcg ctgatcagcg tggttccgcc cgtcactgag ctgcggatgt
     5281 ccgtgcccgg ctccttcaac acgggggcgg actcgcccgt gatcgctgcc tcgttgacga
     5341 agccgacgcc ttcgatgacg tcaccgtcgc ccgggatgac ctcgccctcc tcgacgacga
     5401 tcacgtcgcc cttgcggagc tccgaggatc cgacggcctc cagcgtcccg tcgtcccggc
     5461 gccgatgcgc ggtcgtctcg gaccgggtcc ggcgcagggt cgccgcctgg gcgcgtcccc
     5521 gtgcctcggc gaccgcctcg gcgtaggtcg cgaacaggac cgtgaaccag agccagacgc
     5581 cgatctggag ctggaacccg ctgccggagg tccctgggtc gggctggatg ccggtgaccg
     5641 aagcgatcca gatcagcgtg accagcgccg ccgtgatctc gaccacgaac atcaccgggt
     5701 tgcggatcag cagcctgggg tcgagcttgc ggacggcgtg gggtagggcc gaccggagga
     5761 gctccgggtc gaggatgccg cgcggccggg gcggctcacc ggacggcttc gcgccgccca
     5821 gggtcacgaa gcgctggaag cggcgcgtgc cgcgtcccag gtcaacggac ataagggatc
     5881 gactgaggct catggggtca ggacaccttt cccgcgttct ggatcagttg ctcgacgatc
     5941 gggcccagcg agagggcggg aaagaacgtg agcgcaccga cgatgatcac gacgccgatc
     6001 agcagcaccg tccacagcgg gcccgtggtc gggaacgtcc caagcgatgg tgcgacgcgg
     6061 cgcttggcgg ccatcgagcc cgccagggcg aggatcggga cgatcatccc gtagcgcccg
     6121 atgagcatcg cgatcgcgcc ggtgacgttg tagaagggcg tgttgccggt caggccggcg
     6181 aaggccgagc cgttgttgcc ggtctggctg gagaaggcat agaggatctc gctgaagcca
     6241 tgcggccccg tgttcagcgg gccggcctgg ccctcgggcg tcgccgcggc gagggccgtg
     6301 aacccgagga tgctcgcgcc gaggaccagc gcgaccagca tcgccatctt gatctcgaat
     6361 gcctcgacct tcttgccgag gtactcgggc gtccggccca ccatgaggcc ggcgatgaac
     6421 acggccagga tcgcgccgat gatcagcatc ccgtacatgc cggcgccaat gccgcccggg
     6481 gtgacctcgc cgagctccat gttgaacagc gggacgaggc cggcgatggg ctgggcgctg
     6541 tcgtgccagg agttgatcgc gcccgtactg gtgccggtcg tgacggccat gaacaggccg
     6601 ccgagcgcgg acccgaagcg gacctccttg ccttccatgt tgcccaggcc cgcctggtcg
     6661 gccgcggcgg ggaacagcgg attgcccacg tactcgacgt gcatcgcgcc cacggcgccg
     6721 atcgagagga cgatcgccat cgcggcgaag atcgtccagc cctggcgctg gtcgccggcg
     6781 taccgcccga acgcgtaggt cagggcaaac gggaagagca ggatggcgaa catctccagc
     6841 cagttggtga gcgccgtggg gttctcgaac ggatgggccg agttcgcgtt catgaaccca
     6901 ccgccgttgt tacccagctc cttgatccac tcctgggagg cgacaggccc gatcgcaatg
     6961 tgctggaccg cgccctcgag tgtcgtgacg gcctgggctg ggttccaggt ctggggagcc
     7021 ccctgccaca cgagcacgag ggcaccgatg atcgagatcg ggaggagcag gtacaggatc
     7081 ccgcgcgtca ggtcgaccca gtagttgccg atggtcgtcg cactgcggcg cgtcagcccg
     7141 cggaacaggg cgacggcgat ggccaggccg gtcgcggcgg acgtgaagtt gcggacggcg
     7201 aggacgcccg cctgggtgag gtaggtcgcc cccgtctcgc ccgagtagtt ctgccagttg
     7261 gtgttggtct cgaagctgac cgaggtgttg aaggccaggt ccggcgacat ggcggggatc
     7321 ccgccctggt tgaggggcag gacgtcctgc aagcgaagca cgatgtagcc ggcggcgatg
     7381 gcgaccgccg cgagcacgag gacggcgatc gtgtagccgc gccagccctg ctcggtcgac
     7441 tcgtcgacgc cgcacacgcg gtacaccgcg cgctcgaccg gtcgcaggat cgggctcagg
     7501 aagacgcgtt cgccctccat gacccggtgg atgtaggcgc ccagcagcgg cgtcgcgacc
     7561 aggagagcga cggccagcgt gaacaggacg aacaggtctc cgctacccat ggctcagaag
     7621 tcctcggggc gcaggagtgc ccacaggagg tacacgaaca ggaacgcggc gatcagcgcc
     7681 gtcaggacct cgaggacgtt catcggcgca cgcggtcgca cagggcgacg tagccggcga
     7741 acgcgacgac ggcgccggcg aagatgagca ggatctggac gtctgtcatg gctgctgctg
     7801 agttgcctcc gggttggtga gccgctcgac ggtggccacg agggtctcga gcgggaacgg
     7861 tttcggcagg tagccggcca cgccgaactc gtcgagccac ttcgggctga cggtggtggc
     7921 gctcgtgatg acgacgggcg gccggccggc agcgtccagc gccgcgcctc ggagcaggtc
     7981 ccagcccgtg tcgccgggca ggttcaggtc gagaatcacg acgtcggggc gcaggccgcg
     8041 atcgagcgcc tcgaccgccg cctcggcgga gtcggcctcg gcaacccgaa agcccaggct
     8101 gcgcaggtgg cgcacgacga tccggcgcag gctctcgtcg tcctcgacga tcaggatctg
     8161 ccgcggacgc tcaggcatcg ggcatccgct cgatggcgat gtcggccccg gcgtcgtcgg
     8221 gcatccacag cgggacgacg agcacgcccg ccgcgccggc cagctcgcga gccggctcga
     8281 tgagcgcctc gccgaactgg aggcccccga cgaccacgcg gggcgcgccg cccccggcga
     8341 ccatgtcgat cgcctcccga accaggagaa gctcctgttc gagcgtgcgt ccggccgacg
     8401 gaacgtggcg ggctgaggcg tccatgcctc caccctgcca gcgccctcca agaggcggcg
     8461 ggggtgtgag ggtccgggaa gcctaaggtc ggtctaaagc cgtgggccgg tcgagctcgc
     8521 ggacgcggta gccgacgcct ggctccgtga cgatcaggtc gcgcagcgcg ccacccgggt
     8581 cggccgcggc gatcttgcgc cgcagctggc tgacgtggac gtagatgtag gcgtcctcgc
     8641 cctggtatgc ctcgccccag accgcgcgga gcagccggcc tttcgtgacc aggcgacccg
     8701 cgttggtcag cagcacgcgc aggatctcga actcgcgggg cgtgaggtcg agcgggacgt
     8761 cgcccactcg ggccgcgtgg cgaccggcgt cgaactcgag gggtcccgca cggatcgtgc
     8821 cggcgacgtc ggccaccggg ccggccgcgt ggcgcagggc cacctgcaaa cgagcgttga
     8881 gctcgtcgac gccgaacggc ttggtgacgt agtcgtctgc gccccgctcg agcgcttcga
     8941 ccttctcccg ttcctcatag cgtccagaca ggatcacgat gggcgtcgcg gcctctcgcc
     9001 ggatttggcg cacgatgtcg agtccgtcca tgtcgggcag tccgaggtcg agcaggacga
     9061 ggtccgggcg ccggctctcc caggcgcgga gggccgcgcg tccatcggcc gcctcgaccg
     9121 tccggtagcc gcgaaccgcc agctcccgcc gtagcgccgc ccgtgtctcc tcgtcgtcct
     9181 cgacgacgag gaccgtcggg ccgcctgcag caggccgggg cgatgtcatc cgggcacagc
     9241 cggcgccggc tcgggcgctg tcgcggccgg caggtcgatg tcgatcgcca ggcctcccag
     9301 ctcgcttctc cgggcgtcga cgcggccgtc catcgcctcg acgagccccc tgacgacggc
     9361 gagcccgatc ccgcttcccg accgggagcc gccgggcgtg cccggcaccc ggtagaactt
     9421 gtcgaacagc aggggcagcg tc
//
LOCUS       DBNHXO010000051         2222 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1600720, whole genome shotgun sequence.
ACCESSION   DBNHXO010000051 DBNHXO010000000
VERSION     DBNHXO010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1600720"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..1147
                     /locus_tag="AC162A_01165"
     CDS             335..1147
                     /locus_tag="AC162A_01165"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5009473.1"
                     /translation="MTAEAPAAAAAPRSPEPRGNRPPILEAIDLRKEFRLAGETVDAV
                     RGVNLRVTGGEFIALMGPSGSGKSTLLQLLGGLDQPTSGEVMLEGQPIGRLSDDEATR
                     LRREHTGFVFQSFNLVPLLNVVENVGLPFTIAGLDPRSERLAARVREAIALVELTGKE
                     RHRPDQLSAGEQQRVAVARATVTRPLLLFADEPTGNLDFTTGLGILDALWRTCVDLGQ
                     TVVLVTHDAKTAAYADRVLVLGDGRIRDEIVLGRRDDHTAAPLIERLAQLGL"
     gene            1223..>2222
                     /locus_tag="AC162A_01170"
     CDS             1223..>2222
                     /locus_tag="AC162A_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009474.1"
                     /translation="MLTAFGIAIGVAVLVAALAVDAGLDASIERTVAAQVGRADLRLT
                     AFAEAGLSGRTLAAVDAVPGVAVAAPAIERHTFLLTSSGRTSDGGPVAILGIDAGREL
                     RVRDLDVVRGWAPTKLAPDEVLVSERLAAADGLEVGDDLTLLGAGAPVRTRIGGVLAG
                     DGPAAGSLGRTIVTSLEIATELSTPDGGGLASADPTALRGLSRIDIVLAAGADPAAVE
                     AELERTLTTEPYLLTSPNDVATALRATTADVRATMALLAAISLFAAAFVILNTIAMTV
                     VERIRELGLLRAAGATRAQVGRIVVTQGLLLGALGSALGIVAGALLAAVAATWLRTA"
BASE COUNT          271 a          844 c          806 g          301 t
ORIGIN      
        1 gcgtcctgga cggcatcgcg cgcgagctcg ccgtcccgga ggatcaggcg ggcggccccg
       61 tccagccgcg cgacggccgc gcccgcaagc accgcgaagg cgtccttgtc gccccgctgt
      121 gcccgcatga cgaggtctcg ctggtccacg gtcgctccgc gttcgacgcg gctcacatgg
      181 tcgcgttcgg atgctaatgg cgttcgaccg cgcccgaaag ttcccggcgt gcccgtggct
      241 aggactttcg ggccgcagac cgcaccggac cgcaccgcta gcgtacggac ccgacacacc
      301 acgacaccgc gagcctggag caactcaccc caccatgacc gccgaagccc ccgccgcggc
      361 cgctgccccg cgcagccccg aaccgcgcgg caaccggccg ccgatcctcg aggccatcga
      421 cctgcgcaag gagttccgcc tcgccggcga gaccgtcgat gccgtgcgcg gcgtcaacct
      481 ccgggtgacc gggggcgagt tcatcgcgct gatgggcccc tcgggctccg gcaagagcac
      541 gctgctccag ctgctgggcg ggctcgacca gccgacgtcg ggcgaggtca tgctcgaggg
      601 tcagcccatt ggccggctgt ccgacgacga ggccacccgc ctccggcgcg agcacacggg
      661 cttcgtgttc cagtcgttca acctggtccc gctgctcaac gtcgtggaga acgtgggcct
      721 gccgttcacg atcgcgggcc tcgaccctcg ctccgagcgg ctcgccgcgc gggtgcgcga
      781 ggcgatcgcc ctggtcgagc tgaccggcaa ggagcgccat cggcccgatc agctgtccgc
      841 gggcgagcag cagcgtgtgg cggtggcgcg cgcgacggtc acccggccgc tgctcctgtt
      901 cgccgacgag ccgacgggca acctcgactt caccaccggc ctcggcatcc tcgacgccct
      961 gtggcggacg tgcgtcgacc tcggccagac cgtcgtgctg gtcacccacg acgccaagac
     1021 ggccgcgtac gccgaccggg tcctggtgct gggcgacggg cggatccggg acgagatcgt
     1081 cctgggccgc cgcgacgacc acacggccgc gcccctgatc gagcggctcg cgcagctcgg
     1141 tctgtaaggc ctcggaccca gccgcatgcg aggtctcgtt ccctacgcct ggcgaagcct
     1201 cgtcgcgcga cccgcgcgga ccatgctcac ggcgttcggg atcgccatcg gggtcgccgt
     1261 cctggtggcc gcgctcgccg tcgacgcggg cctcgacgcc tccatcgagc gcaccgtggc
     1321 ggcccaggtc gggcgggcgg atctgcggct cacggcgttc gcggaggccg ggctctccgg
     1381 tcgcacgctg gccgcggtag acgcggtacc gggcgtcgcg gtcgccgcgc ctgcgatcga
     1441 gcggcacacc ttcctcctga cgagctccgg gcgaacgagt gacggcgggc ccgtggccat
     1501 cctcggcatc gacgccggcc gggagcttcg tgtccgcgac ctggacgtcg tgcgcggctg
     1561 ggcccccacg aaacttgccc ccgacgaggt cctggtgagc gagcggctcg ccgccgcgga
     1621 tgggctcgag gtcggcgacg acctgacgct gctcggcgcc ggggcgccgg tgcggacgcg
     1681 gatcggtggc gtgctggccg gcgacggtcc ggccgcgggg tcgctgggcc gcacgatcgt
     1741 cacctcgctc gagatcgcga ccgagctctc gacgcccgac ggcggcggcc tcgcatcggc
     1801 cgacccgacc gcgttgcgcg gtctatcgcg catcgacatc gtgctggcgg cgggcgccga
     1861 cccggccgcc gtggaggccg agctcgagcg gacgctgacc accgagccct acctcctgac
     1921 gtcgcccaac gacgtggcga cggcgctgcg cgcgacgacg gccgacgtgc gcgcgacgat
     1981 ggcgctgctg gccgcgatca gcctgttcgc ggcggcgttc gtcatcctga acaccatcgc
     2041 catgacggtg gtcgagcgca tccgcgagct gggcctcctg cgcgcggcgg gcgcgacccg
     2101 cgcccaggtc gggcggatcg tggtcaccca gggcctgttg ctcggcgcgc tgggctcggc
     2161 gctggggatc gtggccgggg ccctgctcgc ggcagtggcc gctacctggt tgcggaccgc
     2221 gg
//
LOCUS       DBNHXO010000052         2018 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4379087, whole genome shotgun sequence.
ACCESSION   DBNHXO010000052 DBNHXO010000000
VERSION     DBNHXO010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4379087"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1519)
                     /gene="glmM"
                     /locus_tag="AC162A_01175"
     CDS             complement(185..1519)
                     /gene="glmM"
                     /locus_tag="AC162A_01175"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR5009475.1"
                     /translation="MTRLFGTDGIRGVANVDLRPSLAFALGKATAHQVVGAGGSLVVG
                     QDTRRSGDMFVAAITAGATSMGADVHQVGVVPTPALAFLAADPAFAAGIMVSASHNPA
                     EDNGLKVLDPRGLKLDDDVEDELEAAVLRADELPGVAPALIGRTVDGTGLLARYVAHR
                     RALAGAIDASGLHVLLDTANGAAFHVAPEILRATGARVTVIHDAPDGDNINVASGATA
                     PASLAEAVKAAGADVGFALDGDADRCIAVDALGNLVDGDQVLGILALERLARGALDHG
                     SLVVSVLSNGGLQAAVEAAGGRIVRTPVGDKHILAAMLVSGAGLGGEKSGHVIIREHT
                     TSGDGIVTAVELLRVMTAAGSGIAELASVVPLLPQQQRAIRVRHRDQWEADDVLGASI
                     ADAERRLAPHGRVLVRPSGTEPVLRVMVEGPDAALVAELADAIAALAGDRLH"
     gene            complement(1554..>2018)
                     /locus_tag="AC162A_01180"
     CDS             complement(1554..>2018)
                     /locus_tag="AC162A_01180"
                     /inference="COORDINATES: protein motif:HMM:NF019561.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR5009476.1"
                     /translation="DQLATLMQVETDPIPVSGATRDVTMAVALALPTGVVEVEPGPVR
                     VTVHIEVITETRTYSAGIRLDGREPTLQYELPVSQAQLTLFGPVADLDRLGSAPIVVA
                     VSVAGLAPGTHELPVVPSLPSTVTLVEVAPATVTVTITEPVSEASLQPSPAP"
BASE COUNT          262 a          749 c          742 g          265 t
ORIGIN      
        1 acgaacaggt caccctgctc gtcgacgagg gcgatgccgg ccgagtcgta gccccggtac
       61 tcgagacgcc gcagaccctc gatcaggatt gggcccgcct cgcggggccc cgtatagccg
      121 acgattccgc acatgcgcct ggggggtctc cttgggctcg agctcgcggc cccgccgcgg
      181 ctggctagtg tagtcggtcg cctgcgagcg ccgcgatggc gtcggcgagc tcggcgacca
      241 gcgccgcgtc cggaccttcg accatcaccc gcagcacggg ctccgtgccc gacggacgga
      301 cgagcacgcg cccgtgcggc gccagccgtc gctccgcgtc ggcgatcgac gccccgagga
      361 cgtcgtcggc ctcccactga tcgcggtggc ggacccggat cgcccgctgc tgctggggca
      421 ggagcgggac gacgctcgcg agctccgcga tgcccgatcc ggccgcggtc atgacccgca
      481 gcagctcgac ggcggtcacg atcccgtcgc ccgacgtcgt gtgctcgcgg atgatcacgt
      541 ggccgctctt ctcgccgccc agcccggcgc cggacacgag catcgccgcg aggatgtgct
      601 tgtcgccgac cggcgtgcgc acgatccgcc caccggccgc ctccacggcg gcctggagcc
      661 cgccgttcga gagcacggac acgacgaggc tgccgtggtc gagcgcgccg cgcgcgagcc
      721 gctcgagggc gaggatcccg agcacctggt cgccgtcgac gaggttcccg agcgcgtcga
      781 ccgcgatgca ccggtccgcg tcgccgtcga gggcgaaccc gacgtccgcg ccggcggcct
      841 tgaccgcctc ggccagcgag gccggcgcgg tggcgccgct cgcgacgttg atgttgtcac
      901 cgtcgggcgc gtcgtggatc acggtcaccc gcgcgccggt ggcccgcagg atctcgggcg
      961 cgacgtggaa cgccgcgccg ttcgccgtgt cgagcagcac gtgcagcccc gacgcgtcga
     1021 tggcgccggc gagggcccgc cggtgcgcga cgtagcgcgc gaggagccca gtcccgtcca
     1081 cggtccgccc gatcagggcc ggcgccacgc ccggcagctc gtcggcccgc aggacggccg
     1141 cctccagctc gtcctcgacg tcgtcgtcga gcttgaggcc gcgcgggtcg aggaccttga
     1201 gcccgttgtc ctcggccggg ttgtgggacg ccgagaccat gatcccggcg gcgaaggcgg
     1261 ggtccgcggc gaggaacgcg agcgccggcg tggggacgac ccccacctgg tggacgtccg
     1321 cacccatgct tgtcgcgccg gcggtgatcg cggcgacgaa catgtcgccc gaccggcgcg
     1381 tgtcctggcc cacgaccagc gagcctccgg cgccgacgac ctggtgggcg gttgccttgc
     1441 ccagcgcgaa cgcaagcgag ggccgcaggt cgacgttggc cacgcctcga atgccgtccg
     1501 tgccgaacaa ccgtgtcatc ggggtcctcg cgtgatgggg cggtgcgtcc gggtcagggc
     1561 gcgggcgatg gctgaagcga tgcctcgctc accggctccg tgatcgtgac ggtcaccgtt
     1621 gccggtgcca cctccacaag ggtcacggtc gacggcagcg atgggaccac gggcagctcg
     1681 tgggtcccgg gcgcgaggcc tgcgacgctg accgcgacga cgatcggcgc cgacccgagc
     1741 cggtcgaggt cggcgaccgg gccgaacagg gtcagctggg cctgcgacac gggcagctcg
     1801 tactggagcg tcggctcccg gccatcgagt cggataccgg ccgagtacgt tcgcgtctcg
     1861 gtgatcacct cgatgtgcac ggtcacgcgg acgggacccg gctcgacctc caccaccccg
     1921 gtcggcagcg caagggccac ggccatcgtc acgtcccgcg tggccccgga gacggggatg
     1981 ggatccgtct cgacctgcat cagcgtcgcg agctggtc
//
LOCUS       DBNHXO010000053         6380 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_608694, whole genome shotgun sequence.
ACCESSION   DBNHXO010000053 DBNHXO010000000
VERSION     DBNHXO010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_608694"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="AC162A_01185"
     CDS             <1..227
                     /locus_tag="AC162A_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015748802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIR5009477.1"
                     /translation="TIGGFIIAASMIPFLWNVLVSLRNGKPAGDDPWEGNTLEWATSS
                     PPPPYNFDHLPEIRSERPVFDGRHGRTQAH"
     gene            229..894
                     /locus_tag="AC162A_01190"
     CDS             229..894
                     /locus_tag="AC162A_01190"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR5009478.1"
                     /translation="MSDVMTADAELAQHEVLPSHAAGGISNPVLGMLLFITSEIMFFA
                     GLFAAYFSLRAGYQVAGVHVWPPPEFAEILNPFTLFTENGQLNLILPATIILVISSFT
                     CQMGVWAIRRDDHRGFVRNMTATLALGITFLLMQAFDYSVLFHEGLSMGSTPFGTTYF
                     TLTGFHGAHVFGGALMLAVVVYRGMAGQFSSKHHDMVEATSLYWHFVDVVWILLFSIL
                     YLL"
     gene            914..1096
                     /locus_tag="AC162A_01195"
     CDS             914..1096
                     /locus_tag="AC162A_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009479.1"
                     /translation="MSPRRAALVGVIFVVIAFVYWLTPRIAGFPEDYAGITMLLALGV
                     AMSIMTFVLFAGLRRG"
     gene            1110..2249
                     /locus_tag="AC162A_01200"
     CDS             1110..2249
                     /locus_tag="AC162A_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5009480.1"
                     /translation="MIEEAWSQLIGFTEQFVVPDWGSLVGLIPILLLVLAFLYVTWTI
                     YRFATAGPTRRGKRRLTPVPPPGTHLPGGSFAPLIGAFGVLMFGFGLVSGGPWIIVGA
                     VVLTIALLYWGREFLREFDQLPSSSTALIAAGGQALVPGAPMLAPGALPAPEGTPPAG
                     VHIPAPSFRPLLVAMSMTLLVAGMILGGWALILGAVALSFTLFGWLIDARREYVYAVL
                     ADRTGHLDPGPSPSWPTATFAVLAWILAGGLLLSSGLLPNSSAPTTGAAVASAVAGGG
                     GAIPSPAAPSLPAADVVETAENTTFLVGTLEAPAGRPFTIAFDNKDTQPHNIEIKDAS
                     GGVAFNGEIFTGPKVVVYNVPALAAGTYPFVCTVHANMTGTLTTK"
     gene            2299..2772
                     /locus_tag="AC162A_01205"
     CDS             2299..2772
                     /locus_tag="AC162A_01205"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR5009481.1"
                     /translation="MLLVAAVLVAACGGSEGARSGQPAPPITGTALDGTTIDLAAYRG
                     HPVVVNFWASWCTPCREEFPLFEERLATLGPTDGLVILGVLYKDQAELARQFTDEFGA
                     TWPTVTDPGDTIAKAYRVVAPPQTYFIDADGVLQAIQIGQVLPQDFDTQYAKIAP"
     gene            2769..3734
                     /locus_tag="AC162A_01210"
     CDS             2769..3734
                     /locus_tag="AC162A_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5009482.1"
                     /translation="MTGAAGTTADPAVRVEGLRKAYAGREILSGVSFDVRRGELFALL
                     GPNGAGKTTTVEILEGYRRPDAGTASVLGLDPARDGPRLRPRIGLMLQEGGVDNRSTA
                     REVVRLYARFYRDPEDPDRLLDAVDLGRVAGTRYRRLSGGEKQRLALALALLGRPELL
                     LLDEPTAGMDPAAKQATRQRIVDLRAAGTTIVLTTHELADVERLADRVAVLDRGRLVA
                     TGTPAELTGGGAPRLRLRLSRALDEAEAEALTAVVSRIAGGQPGLRAGAPAGSYEVAG
                     MTGAPDPRLVAAIAAWCEARDILVVELHTGAASLEERYLELVGES"
     gene            3731..4507
                     /locus_tag="AC162A_01215"
     CDS             3731..4507
                     /locus_tag="AC162A_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009483.1"
                     /translation="MTAIGTGVPARPAPRTSMVLALTRHEVRMGLRRGEALLITFVIP
                     VGVLLTFSAFGPIGITTTRPVDKVLPGAISLAVIAAAFVALAISTGFERQYGVLKRLG
                     GSPAGSTVVVAAKTASVVLVELVQLLLLVGVAAVVLGWSAGPTAAAAIVLAALVLGTI
                     AFAGLGLLLAGTLRAEATLAFANLLFLVFLVLGGIVVPLDRLPDAVARIAGALPAAAL
                     TQALAIGLGHAPGDAGPPLALLAGWAVVLAALAAWRFRWD"
     gene            4533..5159
                     /locus_tag="AC162A_01220"
     CDS             4533..5159
                     /locus_tag="AC162A_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR5009484.1"
                     /translation="MPDTYADLVEQLADVGRSAVGRGLVVASGGNLSARCPDRDAFLV
                     TGSGTWLDRLAPGDFALVGLDGAPLDGPEPSSEWRLHRATYRVRPDVNAVVHLHPQTA
                     VMLDALGHRVRVLTLDHATYLRRIERVPYYPNGSDELADASAEASRECDVIVLAHHGC
                     STLGDTVEMAWRRALNLEEAALNTFRCLQLGDTDTTFPPDQLETVRHR"
     gene            5288..>6380
                     /locus_tag="AC162A_01225"
     CDS             5288..>6380
                     /locus_tag="AC162A_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR5009485.1"
                     /translation="MQQLITRVQAALRNVTEDSRSHRLIAWLRPAVLVVASVLVLGAA
                     FITADDAAHRVTEAAGIESARSAETIVRASVDPLLAEASMGDPDGSSGREINSLLERL
                     VQPGGIARIKLWSPAGTVLFSDLSDIRGRVFEVDDKLRAALEGETASELHYGDSEAPE
                     NAFDIGLPEDYLEIYLPVRASNGTVIGAYEIYEDASSIIELIEATRRDVFIVAGLAAT
                     VLLLMLWSAFNATSKTLASQNRRLVELAADLRGREARSRSLLQNSSDAQAILDADGRV
                     RYESVAVERILGHAAADWEGRPFASVVHDDDRATVEQALADLASGQGSERRFESRVKH
                     ADGTWRTIEAIGRNLLEDPAVAGIVINHRD"
BASE COUNT          819 a         2363 c         2211 g          987 t
ORIGIN      
        1 cgacgatcgg cgggttcatc atcgcggcga gcatgatccc gttcctgtgg aacgtcctcg
       61 tgtcgctccg caacggcaag ccggccggcg acgacccgtg ggagggcaac acgctcgagt
      121 gggccacgtc gtccccaccg ccgccctaca acttcgatca cctgccggag atccgctccg
      181 agcggcccgt gttcgacggc cggcacggcc ggacccaggc gcactgacgt gagcgacgtg
      241 atgacggccg acgcggagct cgcccagcac gaggtcctgc ccagccacgc cgccggcggc
      301 atcagcaacc cggttctcgg gatgctgctg ttcatcacct cggagatcat gttcttcgcc
      361 ggcctgttcg cggcctactt cagcctccgg gcgggctacc aggtggccgg cgtccatgtc
      421 tggccgccgc ccgagttcgc cgagatcctg aacccgttca cgctgttcac cgagaacggc
      481 cagctgaacc tgatcctgcc ggcgacgatc atcctcgtga tctcgtcgtt cacctgccag
      541 atgggcgtgt gggcgatccg ccgcgacgac caccgcgggt tcgtgcgcaa catgacggcg
      601 accctcgccc tgggcatcac gttcctgctg atgcaggcgt tcgactactc ggtcctgttc
      661 cacgaggggc tgtcgatggg ctcgaccccg ttcggcacca cctacttcac cctcaccggc
      721 ttccacggcg cccacgtgtt cggcggcgcg ctgatgctcg cggtcgtcgt gtaccggggc
      781 atggccggcc agttcagctc caagcaccac gacatggtcg aggcgacgtc gctctactgg
      841 cacttcgtcg acgtcgtctg gatcctgctg ttctcgatcc tgtacctgct gtagcgcgga
      901 ggagctgttt tcgatgtccc cccgtcgcgc ggcgctggtc ggcgtgatct tcgtcgtgat
      961 cgccttcgtc tactggttga caccgcgcat cgccggcttc cccgaggact acgccgggat
     1021 cacgatgctg ctcgccctcg gcgtggcgat gtcgatcatg accttcgtgc tgttcgccgg
     1081 cctgcggcga ggctgacgat ctcgcggcga tgatcgagga ggcctggagc cagctcatcg
     1141 gcttcaccga gcagttcgtc gtccccgact gggggtcgct ggtcggcctc atcccgatcc
     1201 tgctgctcgt gctcgccttc ctgtacgtga cctggacgat ctaccggttc gcgaccgccg
     1261 ggcccacgcg ccgcgggaag cgccggctga ccccggtgcc gccgcccggg acgcacctgc
     1321 cgggcgggtc gttcgcgccg ctcatcggtg cgttcggggt cctgatgttc gggttcgggc
     1381 tggtctccgg cgggccgtgg atcatcgtgg gcgccgtggt cctcacgatc gcgctcctct
     1441 actgggggcg tgagttcctg cgcgagttcg accagctgcc atcgtcctcg acggcgctca
     1501 tcgccgccgg cggtcaggcc cttgtcccgg gtgccccgat gctggcgccg ggagccctcc
     1561 cggcgccaga ggggacgccg cccgcaggcg ttcacattcc cgcaccctcg ttccgcccgc
     1621 tgctcgtggc gatgtcgatg accctgctcg tggccgggat gatcctcggc ggctgggccc
     1681 tgatcctcgg ggcggtcgcg ctctcgttca cgctgttcgg ctggctgatc gatgcccgcc
     1741 gcgagtacgt ctacgcggtc ctggccgacc ggaccgggca cctcgatccg ggcccgtccc
     1801 cgtcgtggcc gacggcgacc ttcgccgtgc ttgcctggat cctcgcgggc gggctcctgc
     1861 tcagctccgg gctgctgccc aactcgagcg cgccgacgac cggtgcggcc gtcgcaagcg
     1921 cggtggccgg cggcggtggc gccatcccgt caccggccgc accgagcctg ccggccgctg
     1981 acgtggtcga gaccgccgag aacacgacct tcctcgtcgg gacgctcgag gcgccagccg
     2041 gcaggccgtt cacgatcgcc ttcgacaaca aggacacgca gccccacaac atcgagatca
     2101 aggacgcgtc ggggggcgtc gcgttcaacg gcgagatctt cacggggccc aaggtcgtgg
     2161 tctacaacgt gccggcgctg gccgcgggca cctacccgtt cgtgtgcacg gtccacgcga
     2221 acatgacggg cacgctgacc accaagtagg cgcgtcacga accgcacaac ccaccctcga
     2281 acgggccccc tggcggtcgt gctgctcgtc gctgcggtgc tcgtcgccgc ctgcggcggg
     2341 tccgagggag cgcggagcgg ccagcccgcc ccgccgatca ccggcacggc cctggacggc
     2401 acgaccatcg acctcgccgc gtaccgcggg cacccggtgg tcgtgaactt ctgggcgtcc
     2461 tggtgcacgc cctgccgcga ggagttcccg ctgttcgaag agcgcctcgc gacgctcggg
     2521 ccgaccgacg ggctggtcat cctgggcgtg ctgtacaagg accaggcgga gctcgcgcgc
     2581 cagttcaccg acgagttcgg ggcgacctgg ccgacggtca ccgatccggg cgacacgatc
     2641 gccaaggcgt accgcgtcgt ggccccgccg cagacctact tcatcgacgc cgacggcgtc
     2701 ctgcaggcga tccagatcgg ccaggtgctg ccccaggact tcgacaccca gtacgcgaag
     2761 atcgcgccgt gacgggcgcg gccgggacca ccgcggatcc cgccgtccgt gtcgaggggc
     2821 tgcgcaaggc ctacgccggt cgcgagatcc tcagcggcgt ctcgttcgac gtgcgacgtg
     2881 gcgagctgtt cgcgctgctg gggcccaacg gcgcgggcaa gacgacgacg gtcgagatcc
     2941 tcgagggcta ccgccggccc gacgccggga cggcgtcggt gctcggtctc gacccggcac
     3001 gggacggccc gcgcctgcga ccgcgcatcg ggctgatgct ccaggaagga ggggtcgaca
     3061 accgctcgac ggcccgcgag gtcgtccggc tgtatgcgcg cttctatcgt gacccggagg
     3121 accccgaccg cctgctcgac gccgtcgacc tgggccgcgt ggccggcacg cgctaccggc
     3181 gcctgtcggg cggcgagaag cagcgcctgg cgctggcact ggccctgctc ggccggccgg
     3241 agctgctgct cctcgatgag ccgactgccg ggatggaccc ggccgccaag caggccacgc
     3301 gccagcgcat cgtggacctg cgcgccgccg ggacgaccat cgtgctcacg acccacgagc
     3361 tggccgacgt cgagcggctc gccgaccggg tcgccgtgct cgatcggggc cggctggttg
     3421 cgacgggcac gcccgccgag ctcaccgggg gcggggcgcc ccgcctgcgg ctccggctct
     3481 cgcgtgcgct cgacgaggcc gaggccgagg ccctgacggc cgtcgtgagc cggatcgccg
     3541 ggggacaacc gggcctgcgc gcgggagcgc cggcgggctc gtacgaggtc gcggggatga
     3601 caggcgcacc cgacccacgc ctggtcgccg cgatcgccgc gtggtgcgag gcgcgcgaca
     3661 tcctcgtcgt cgagctgcac acgggcgccg cgagcctcga ggagcgctac ctcgagctgg
     3721 tgggcgagtc atgaccgcga tcgggaccgg ggtcccggcg cgcccggctc caaggacgag
     3781 catggtcctc gcgctcaccc ggcacgaggt acggatgggc ctccgccgtg gcgaggcgct
     3841 gctgatcacg ttcgtgatcc cggtcggcgt gctgctcacg ttcagtgcct tcggcccgat
     3901 cgggatcacc acgaccaggc cggtcgacaa ggtgctgccg ggggccatca gcctcgcggt
     3961 gatcgccgcc gccttcgtcg ccctcgcgat ctcgaccggc ttcgagcgcc agtacggcgt
     4021 gctcaagcgc ctcggcggca gcccggcggg atcgacggtc gtggtcgcgg ccaagacggc
     4081 gtcggtcgtc ctggtcgagc tcgtccagct ccttctcctg gtcggggtcg ccgcggtcgt
     4141 cctcggctgg tccgccggcc cgaccgccgc ggccgcgatc gtgctcgcgg cgctcgttct
     4201 cgggacgatc gcgttcgcgg gcctgggcct gctcctcgcg ggcacgctcc gcgccgaggc
     4261 gacgctcgcc ttcgcgaacc tgctgttcct cgtgttcctc gtgctcggcg gcatcgtcgt
     4321 gcccctcgac cggctgccgg atgccgtcgc ccggatcgcc ggcgcgctgc cggcggcggc
     4381 gctgacacag gccctcgcga tcgggctcgg gcatgcgccg ggcgacgccg ggccgccgct
     4441 cgccctgctc gcgggctggg ccgtcgtgct cgccgccctc gcggcctggc ggttccgctg
     4501 ggactgacgc acccgtcatg attggcggcg acatgccgga cacctacgcg gacctcgtcg
     4561 agcagctggc cgacgtcggg cggtcggccg tcgggcgcgg cctcgtggtc gcctcgggcg
     4621 gcaacctctc ggcccgctgc cccgacaggg acgcgttcct cgtcacgggt tccgggacgt
     4681 ggctggaccg gctggcaccc ggagacttcg ccctcgtcgg cctcgacggg gcgccgctcg
     4741 acggcccgga gccatcgagc gagtggcgcc tgcatcgcgc gacgtaccgc gtccggcccg
     4801 acgtcaacgc ggtcgtccac ctccacccac agacggcggt catgctcgac gcgctgggcc
     4861 atcgcgtccg ggtgctcacg ctcgaccacg cgacgtacct gcggcggatc gagcgtgtcc
     4921 cctattaccc caacggctcc gacgagctcg ccgacgcgtc cgcggaggcc tcgcgcgagt
     4981 gcgacgtcat cgtcctcgcg caccacggct gctcgacgct gggcgacacg gtcgagatgg
     5041 cctggcgccg ggcgctcaat ctcgaggagg cggcgttgaa cacgttccgc tgcctgcaac
     5101 taggcgatac ggacacgacc ttccccccgg accagctcga aaccgtccgc caccgctgac
     5161 cgtttggccc gcgcgacgca ggcgtacttc cggccacgac cggtccggac caacggcggg
     5221 gtcacacccc gaccatcgtc gccctagtgt tgcttcccag ccccactagg cacgagtaca
     5281 ccgcttcttg cagcagctga tcacgcgcgt tcaagccgct ctgcgcaacg tcaccgagga
     5341 ctcgcgaagc caccgcttga ttgcgtggct ccggccggcg gtgcttgtcg tcgcgtccgt
     5401 gctcgtcctg ggcgccgcct tcatcaccgc cgacgacgcc gcccaccggg tcaccgaggc
     5461 ggccgggatc gagtccgcgc ggagcgcgga gacgatcgtg cgcgcgagcg tcgacccgct
     5521 gctcgccgag gcctcgatgg gcgacccgga tggctcgtcg ggtcgcgaga tcaacagcct
     5581 gctggagcgc ctcgtccagc ccggcggcat cgcccggatc aagctctggt cgccggccgg
     5641 cacggtcctg ttctcggacc tgtcggacat tcgcgggcgc gtgttcgagg tcgacgacaa
     5701 gctcagggcg gccctcgagg gcgagacggc gagtgagctc cactacgggg actcggaggc
     5761 tcccgagaac gccttcgaca tcgggctgcc cgaggactat ctcgagatct acctcccggt
     5821 gcgggcgtcg aacggcaccg tcatcggggc gtacgagatc tacgaggacg catcctcgat
     5881 catcgagctg atcgaggcga cgcgacgcga cgtgttcatc gtcgccgggc tggcggcgac
     5941 ggtgctgctg ctgatgctgt ggagcgcgtt caatgcgacc tcgaagacgc tcgccagcca
     6001 gaacaggcgg ctggtcgagc tggccgccga cctgcgtggc cgcgaggctc gttcccggtc
     6061 cctgctccag aactcgtcgg acgcccaggc catcctcgac gcggacggcc gggttcgcta
     6121 cgagagcgtc gccgtggagc ggatcctcgg ccacgcggcg gccgactggg agggccggcc
     6181 gttcgcgtcg gtcgtccacg acgacgatcg cgccacggtc gagcaggccc tggcagacct
     6241 cgcctccggc caagggtcgg agcggcgctt cgagagccgg gtcaagcacg ccgacggcac
     6301 gtggcggacg atcgaggcga tcggccgcaa cctgctcgaa gacccggccg tcgccggcat
     6361 cgtcatcaac caccgcgaca
//
LOCUS       DBNHXO010000054         5539 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1601919, whole genome shotgun sequence.
ACCESSION   DBNHXO010000054 DBNHXO010000000
VERSION     DBNHXO010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1601919"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="AC162A_01230"
     CDS             <1..770
                     /locus_tag="AC162A_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008273 - calcium, potassium:sodium
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006874 - intracellular calcium ion
                     homeostasis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HIR5009486.1"
                     /translation="RKRRATDTRMVVDTEVLVHDIRYFLIAYGLAIATAFLPPDLNLI
                     RYAVAFVLIGIYIWYVKGHFDEEAEEEHDELAPLRFHRMDRSAHQDDPTIPRLRIVNV
                     QVIVALAFIIGGAWLFVGAVEELASTLGVSEILLALVIAPIATELPEKFNSLIWVRQG
                     KDTLAMGNITGAMVFQSAFPTFVALIFAGSAWHISENSLLAFLSAGITFLAVAAIFGP
                     MWRRGRLDGKWLLVGGLLYLIYLVLVGLGIAGVIPTG"
     gene            872..1852
                     /locus_tag="AC162A_01235"
     CDS             872..1852
                     /locus_tag="AC162A_01235"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR5009487.1"
                     /translation="MVTQQSSAATSAGAAPAQARSLDDLIAYFEGNWVPLRDAKVSIM
                     THAFMYGTATFEGIRGYWNAEQGQLYGLFVPEHMERIRNSAKMLLMEDIPSVDELVGI
                     VVETARRNQFREDIYIRPSFYKSTRAIGVRLHHLDHELYVITTPFGNYIDIEKGVRIM
                     TSTWRRNADEALPARGKIVGGYVNMAFQKSEAELNGFDEALVLTPDGHASEASAANLF
                     VVRDGVLLTPPVSDDILEGVTRKAIIQLAQDAGIPVEVRSIDRSELYVCDEVFLCGTG
                     VQVSAVIEVDHRSVGTGAIGPVARLISGRYFDAVRGKLPEYMSWLTPIGE"
     gene            complement(1860..3704)
                     /locus_tag="AC162A_01240"
     CDS             complement(1860..3704)
                     /locus_tag="AC162A_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR5009488.1"
                     /translation="MSTPPKPPAAPRPPRTRSAFAAAFLSLIFPGLGHAYAGAWSRAL
                     AFAALPLLLLALAGGIFLRANRFELLGFIVQPQVLIGVFIVNVLILIYRIVAAIDAWN
                     VARFLNDVDASGGGRLGRSKLPLSPLSVAGLLAVLIVLGGAHLAVARYNVMAIDLVNC
                     VFTDSDDPTCEEPAETPEPGATTTAGATPDASEEPTATEAPLETPIGSATGTIAPELP
                     AWDGKERLNILLVGVDQREGQSYFNTDTLIVASIDPKTNEVAMFQVPRDTVDVPVPAN
                     ARSVWGSVYRGKINAWYANTRNRTDLWRGEKSQTRGFNALKALLGELYNLDIRYYVMV
                     NFQGFRDAVNTLGGVQINVQIPVAESDYPVGGAVTRVYIPAGPQHMSGPQALIYARSR
                     HRAQGGDFDRGRRQQRVLVSLREQMNVQSIMTNLTDLVKTLKQSVNTDIPVAEVPKLL
                     SLADSVDTKNIRSYVFAPSFYATEATEAVRGYIIEPNIVRIRKAVKEAFSTPAELLAL
                     RERLGAEAARVWVLNASGRPGLSARSADRLAYDGMDASAPNQRPNTLANTKIVVYNGA
                     ETTMPETIAYLEDLFNTTVTTAVDPKVTVDMIVTLGRNAPDLEVDPVG"
     gene            3817..>5539
                     /locus_tag="AC162A_01245"
     CDS             3817..>5539
                     /locus_tag="AC162A_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012361.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR5009489.1"
                     /translation="MFEPVSSRPDFVAQEHALLREWTERRTFARLRAQNAGGPRWSFL
                     DGPITANNPMGVHHAWGRTYKDLYQRFHAMLGEDQRYQNGFDCQGLWVEVNVERDLGF
                     TSKRDIEAFGIAEFVSLCKQRVLTFAARQTEQSMRLGYWMDWNDPAELLRLRDLLAAD
                     PSAPATIQGPDGPVSDTVEMVVGRLGMPDVGGSYFTFSNENNDLIWGFLAECHRRGWI
                     YRGHDTMPWCPRCGTGLSQMEMNEGYQDREDPGLTVRFPLLDRPGESLLVWTTTPWTL
                     AANVAAAVGPDLRYVKVRQGEDAFWLSRGTLKQVLQGPFQVVEEVAGADLVGWRYEGP
                     FDELPAVRDAFTEAAYEHRVVAWSEVGEEEGTGIVHIAPGCGAEDFQLGTSLGLPVVG
                     PIDEDGRYYPDFGWLSGREAPAITEAIIDDLEQRRFFYHLEPYSHRYPHCWRCGTPLL
                     FRLVDEWYISMGEVYDQPREALTKAQVDASLRYQIMEVVDRIRWIPSFGYERELDWLL
                     NMRDWMISKKRYYGLALPIYDCRACGTFDVIGGREELETRAVEGWDAFAGHTPHRPFV
                     DEVKIACP"
BASE COUNT          844 a         1884 c         1939 g          872 t
ORIGIN      
        1 tccgcaagcg gcgtgcgacc gacacccgga tggtggtcga caccgaggtg ctcgtccacg
       61 acatccgcta cttcctgatc gcctacgggc tcgcgatcgc gaccgcgttc ctgccgcccg
      121 acctgaatct gatccgctac gcggtcgcct tcgtcctcat cgggatctac atctggtacg
      181 tcaagggcca cttcgacgag gaggccgagg aggagcacga cgaactcgcg ccgctgcgct
      241 tccaccgcat ggaccggagc gcccaccagg acgacccgac gatcccgcgc ctgcgcatcg
      301 tcaacgtcca ggtcatcgtc gcgctcgcct tcatcatcgg cggcgcctgg ctgttcgtgg
      361 gtgccgtcga ggagctcgcc tcgacgctcg gcgtcagcga gatcctgctc gccctcgtga
      421 tcgccccgat cgcgacagag ctgcccgaga agttcaactc actgatctgg gtccgccagg
      481 gcaaggacac gctcgcaatg ggcaacatca cgggcgccat ggtcttccag tcggcgttcc
      541 cgacgttcgt ggccttgatc tttgccggct cggcctggca catcagcgag aactcgctgc
      601 ttgcgttcct gtcggccgga atcacgttcc tcgcggtcgc ggcgatcttc ggcccgatgt
      661 ggcgccgggg gcgcctcgac gggaagtggc tgctcgtcgg cgggctgctg tacctcatct
      721 acctggtcct cgtcggcttg ggcatcgcgg gcgtcatccc gaccggctga gggagctgat
      781 cgtccgggct gacccccgat cgccgcgtgc taggatcggc caacgtacga accccgcggc
      841 ctcgtgccgc atgcgacgtg gagcgccgcc gatggtcacc cagcagtcgt ccgccgcaac
      901 gtcggccggg gcagccccgg cccaggcccg gtcgctcgac gacctgatcg cgtacttcga
      961 gggcaactgg gtaccgctgc gggacgcgaa ggtcagcatc atgacccacg cgttcatgta
     1021 cgggacggcc acgttcgagg gcatccgcgg ctactggaac gccgagcagg gccagctcta
     1081 cgggctgttc gtgccggagc acatggagcg catccgcaac tccgccaaga tgctgctcat
     1141 ggaggacatc ccctccgtcg acgagttggt cggaatcgtg gtcgagaccg cgcgccggaa
     1201 ccagttccgc gaggacatct acatccggcc ctcgttctac aagagcaccc gcgcgatcgg
     1261 cgtccggctc caccatctcg accacgagct gtacgtgatc acgacgccgt tcgggaacta
     1321 catcgacatc gagaagggcg tacggatcat gacctcgacc tggcggcgca acgccgacga
     1381 ggcgttgccg gctcgaggca agatcgtcgg cggctacgtg aacatggcgt tccagaagtc
     1441 cgaggcggag ctcaacggct tcgacgaggc gctggtcctg acgcccgacg gccatgcgtc
     1501 cgaggcctcc gcggcgaacc tgttcgtcgt gcgcgacggc gtgctgctga cgccgccggt
     1561 gtcggacgac atcctcgagg gcgtcacccg caaggcgatc atccagctgg cccaggacgc
     1621 gggcatcccg gtcgaggtcc gctcgatcga ccgctccgag ctctacgtgt gcgacgaggt
     1681 attcctgtgc ggcaccggcg tccaggtcag cgcggtgatc gaggtcgacc atcggtcggt
     1741 cggcacgggc gcgatcggac ccgtcgcgcg cctgatcagc gggcgctact tcgacgcggt
     1801 gcgcggcaag ctgcccgagt acatgagctg gctgacgccg atcggcgagt agcggccggt
     1861 cagccgaccg ggtcgacctc gaggtccggg gcgttccgcc cgagcgtgac gatcatgtcc
     1921 acggtgacct tgggatcgac cgccgtcgtg accgtggtgt tgaacaggtc ctcgaggtac
     1981 gcgatcgtct cgggcatcgt cgtctcggcg ccgttgtaga ccacgatctt ggtgttcgcc
     2041 agcgtgttcg ggcgctggtt gggcgcggac gcgtccatgc cgtcgtacgc gagccggtca
     2101 gccgaccgtg ccgacagccc cggcctgccc gaggcgttga ggacccacac ccgcgccgcc
     2161 tcggctccca ggcgctcgcg cagcgcgagc agctcggccg gcgtcgagaa cgcctccttg
     2221 acggccttgc ggatgcggac gatgttgggc tcgatgatgt agccgcggac ggcctcggtc
     2281 gcctcggtcg catagaacga cggcgcgaac acgtaggagc ggatgttctt ggtgtcgacg
     2341 ctgtccgcga gcgagagcag cttcgggacc tccgcgacgg ggatgtcggt gttgaccgac
     2401 tgcttgagcg tcttcacgag gtcggtcagg ttggtcatga tcgactgcac gttcatctgc
     2461 tcacgcaacg acaccaggac ccgctgctgg cgccggccgc ggtcgaagtc ccctccctgc
     2521 gcccggtgcc gcgagcgggc gtagatgagc gcctgggggc cgctcatgtg ttgcgggccg
     2581 gccgggatgt agaccctggt caccgcgcct ccgaccgggt agtcgctctc ggcgaccggg
     2641 atctggacgt tgatctggac cccgcccagg gtgttcacgg cgtcgcggaa gccctggaag
     2701 ttgaccatca cgtagtagcg gatgtcgagg ttgtacagct cgccgagcag cgccttgagg
     2761 gcgttgaagc cgcgggtctg ggacttctcg ccgcgccaca ggtcggtccg gttgcgggtg
     2821 ttggcgtacc aggcgttgat cttgccccgg tagacgctgc cccagacgct gcgcgcgttc
     2881 gccggaaccg gcacgtcgac cgtgtcgcgc gggacctgga acatcgcgac ctcgttggtc
     2941 ttggggtcga tcgaggccac gatcagggtg tcggtgttga agtagctctg gccctcgcgc
     3001 tggtcgacgc cgaccagcag gatattcagc cgctccttgc cgtcccaggc cggcagctcg
     3061 ggggcgatgg tcccggtcgc gctgccgatg ggcgtctcga gcggcgcctc cgtcgcggtg
     3121 ggctcctcgc tggcgtccgg ggtcgccccc gcggtcgtcg tcgcgcccgg ttccggcgtt
     3181 tccgccggct cctcgcaggt cgggtcgtcg ctgtcggtga acacgcagtt cacgaggtcg
     3241 atggccatga cgttgtagcg cgcgaccgca aggtgcgcgc cgccgaggac gatcaggacc
     3301 gccagcaggc ccgcgaccga cagcgggctc agcggcagct tcgaccgccc caggcggcca
     3361 ccgcccgaag cgtcgacgtc gttgaggaag cgggcgacgt tccaggcgtc gatcgcggcg
     3421 acgatgcggt agatcaggat caggacgttg acgatgaaca ccccgatgag cacctggggc
     3481 tggacgatga acccgagcag ctcgaagcgg ttggcacgca ggaagatccc gcccgcgagc
     3541 gccagcagca gcagcggcag cgcggcgaac gcgagcgccc gcgaccaggc gccggcatag
     3601 gcatgcccga gcccggggaa gatcagggac aggaaggcag cggcgaacgc ggaccgtgtc
     3661 cgcggcgggc gcggcgcggc cggcggtttg gggggagtcg acatcgcgcg gaatgatacg
     3721 tcgcgcaagg aattcgggcg ggcgccgcga gccgatagac tggtcacccg ctcgcaggcc
     3781 cggcccgggc cgcggtccgc gacccctcga ggcccgatgt tcgaacccgt ctccagccga
     3841 cccgactttg tcgcccagga gcacgcgctg ctcagggagt ggacggagcg gcgcaccttc
     3901 gcgaggctcc gcgcccagaa cgccggcggc ccgcggtgga gcttcctcga cgggccgatc
     3961 accgcgaaca acccgatggg cgtccaccac gcgtggggcc gcacgtacaa ggacctctac
     4021 cagcgcttcc acgcgatgct cggcgaagac cagcgctacc agaacgggtt cgactgccag
     4081 ggcctgtggg tcgaggtcaa cgtcgaacgc gacctcggct tcacctccaa gcgtgacatc
     4141 gaggcgttcg ggatcgccga gttcgtgagc ctgtgcaagc agcgggtgct cacgttcgcc
     4201 gcgcgccaga ccgagcagag catgcggctc ggctactgga tggactggaa cgatcccgcg
     4261 gagctgctcc ggctgcgcga cctgctggcg gcggacccgt cagcgccggc gacgatccag
     4321 gggcccgacg gccctgtcag cgacaccgtg gagatggtgg tcgggcggct cggcatgccc
     4381 gacgtcggcg gcagctactt cacgttcagc aacgagaaca acgacctgat ctgggggttc
     4441 ctcgccgagt gccaccgacg cggctggatc taccggggtc acgacacgat gccctggtgc
     4501 ccgcgctgcg gcacgggcct gtcgcagatg gagatgaacg agggctacca ggaccgcgag
     4561 gacccgggtc tgaccgtccg attcccgctc ctcgaccggc ccggggagtc gctgctggtc
     4621 tggaccacga cgccctggac cctggcggcc aacgtcgccg ccgccgtggg gccggacctg
     4681 cgctacgtca aggtccggca gggcgaggat gccttctggc tcagccgcgg cacgctcaag
     4741 caagtcctcc agggcccgtt ccaggtggtc gaggaggtcg ccggggcgga cctcgtgggc
     4801 tggcgctacg aagggccctt cgacgagctg cccgccgttc gcgacgcgtt cacggaggcc
     4861 gcgtatgagc atcgggtggt cgcctggagc gaggtcggcg aggaggaggg gacgggcatc
     4921 gtccacatcg cccccggctg cggcgccgag gacttccagc tcggcacgtc gctcgggctg
     4981 ccggtcgtcg gaccgatcga cgaggacggg cgctactacc cggacttcgg ctggctctcg
     5041 gggcgcgagg cgccggcgat caccgaggcg atcatcgacg acctcgagca acgccggttc
     5101 ttctaccacc tcgagcccta cagccaccgc tacccgcact gctggcggtg cgggacgccg
     5161 ctgctgttcc gtctcgtgga cgagtggtac atcagcatgg gcgaggtcta cgaccagccg
     5221 cgcgaggcgc tcaccaaggc ccaggtcgac gccagcctgc gctaccagat catggaggtc
     5281 gtcgaccgga tccggtggat cccgtcgttc ggctacgagc gcgagctcga ctggctgctc
     5341 aacatgcgcg actggatgat ctccaagaag cgctactacg ggctggcgct gccgatctac
     5401 gactgccgtg cgtgtggcac gttcgacgtc atcggcgggc gcgaggagct cgagacgcgc
     5461 gcggtcgagg gctgggacgc cttcgcgggt cacacgccgc accgcccgtt tgtggacgag
     5521 gtgaagatcg cctgtccca
//
LOCUS       DBNHXO010000055         1885 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2990380, whole genome shotgun sequence.
ACCESSION   DBNHXO010000055 DBNHXO010000000
VERSION     DBNHXO010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2990380"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="AC162A_01250"
     CDS             <1..489
                     /locus_tag="AC162A_01250"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR5009490.1"
                     /translation="IGINTAVAGDSQGIGFAIPIDIARPLLAQASAGQALARPWLGVR
                     FETIDPTVKAAAGLGVDNGAWIPDASALGNGAQGQDPNDPFAQAAAEPAIVPGGPAEK
                     AGLKAGDIITAVNGQALDAQHPLDLVMSQLAPGQTASLTVLRDGSTITIDVPLGTRPS
                     ES"
     gene            complement(544..1527)
                     /locus_tag="AC162A_01255"
     CDS             complement(544..1527)
                     /locus_tag="AC162A_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAS signaling domain-containing protein"
                     /protein_id="HIR5009491.1"
                     /translation="MSTLTDRYVWGVVRAVPESQRPELEPEIRAMIADAIDARTATGS
                     PGEPADAVERAVLTELGDPEVLAAHYTDRTLYLIGPAYFLVWKRLLLMLLPVVVPIVT
                     IVAMAARSFAGDAGIGDVIAAGVTAAINVAIQIVFWFTVVFAVIERTGGKQLDVGTKW
                     TPDQLPGLPVPGRLSLAELALSVAGLVFAAVFIVWQQVAPPITVSGQSYPIFDPALWS
                     FWLPWFLVVIGLELVFTGALYLAGRWTWPFAVVNALLAAAFAIPAVWLIQTDQLFNPA
                     AADALATAGFGGAIAPTGLIIAVSIAVISAWDAVDGFLKASRVSRAATAAA"
     gene            complement(1524..>1885)
                     /locus_tag="AC162A_01260"
     CDS             complement(1524..>1885)
                     /locus_tag="AC162A_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015660777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR5009492.1"
                     /translation="HTIVPAMTFDEILSGTQQELRRGTVVLASLLLLREPGYGYALLD
                     QLRDLGFSVDANTLYPLLRRLETQGMLASAWDTSEARPRKFYRISPDGERLAAELLAD
                     WSRLDAAIGRLNHGDPT"
BASE COUNT          300 a          673 c          677 g          235 t
ORIGIN      
        1 atcgggatca acaccgcggt cgccggcgac agccagggca tcgggttcgc gatccccatc
       61 gacatcgccc gcccgctgct cgctcaggcg tcagccggtc aggcactggc acgcccgtgg
      121 ctcggcgtcc ggttcgagac gatcgatccg accgtgaagg cggcggccgg gctcggcgtt
      181 gacaacggcg cctggatccc cgacgccagc gcgctcggca acggcgctca gggccaggac
      241 ccgaacgacc ccttcgccca ggcggcagcc gagccggcga tcgtcccggg cgggcccgcc
      301 gagaaggccg gcctcaaggc cggcgacatc atcaccgcgg tcaacggaca ggcgctcgac
      361 gcccagcacc cgctcgacct cgtgatgagc cagctcgcgc ccggccagac cgcgtcgctc
      421 accgtcctcc gggatgggtc gacgatcacg atcgacgtcc cgctcgggac gcggccgagc
      481 gagtcctagt cacccgcgca gcacgacggg gctccccatc gggagccccg ttttcgttgc
      541 ccgtcaggcg gccgcggtcg cagcccgcga gacgcgcgac gccttgagga acccgtcaac
      601 cgcgtcccag gcggagatca ccgcgatgga caccgcgatg atcaggcccg ttggcgcgat
      661 cgccccgccg aagccggccg tcgcgagtgc gtcggccgcc gccgggttga acagctggtc
      721 ggtctggatg agccacacgg cggggatcgc gaacgccgcg gcgagcagcg cgttgacgac
      781 ggcgaacggc caggtccagc ggcccgcgag gtagagcgcg cccgtgaaca cgagctcgag
      841 gccgatgacc accaggaacc acggcagcca gaacgaccac agggcggggt cgaagatcgg
      901 gtaggactgg ccgctgaccg tgatcggtgg cgcgacctgc tgccacacga tgaacaccgc
      961 ggcgaacacg aggccggcga cggacagcgc gagctccgcc aggctgagtc gtcccgggac
     1021 cggcagcccc ggcagctgat ccggggtcca cttggtgccc acgtcgagct gcttgccccc
     1081 ggtgcgctcg atcacggcga acacgaccgt gaaccagaac acgatctgga tcgccacgtt
     1141 gatggccgcc gtgacgcccg cggcgatcac gtcgccgatg ccggcgtcgc ccgcgaacga
     1201 cctcgccgcc atcgcgacga tcgtcacgat cgggacgacg accggcagca gcatcagcag
     1261 cagccgcttc cagacgagga agtacgcggg gccgatgagg tacagcgtcc ggtccgtgta
     1321 gtgggccgcg aggacctcgg ggtcgccgag ctcggtcagg acggcgcgct cgacggcatc
     1381 ggcgggctcg ccgggcgagc cggtcgcggt tcgcgcgtct atcgcgtcgg cgatcatcgc
     1441 ccggatctcg ggctcgagct cgggccgttg ggactccggg acggcgcgca cgacgcccca
     1501 gacatagcgg tcggtcaggg tgctcacgtg gggtctccat ggttgaggcg gccgatcgcg
     1561 gcatcgagcc gcgaccagtc ggcgaggagc tcggcggcga ggcgctcgcc gtcggggctg
     1621 atccgataga acttccgggg acgcgcctcg ctcgtatccc acgcgctggc cagcatcccc
     1681 tgggtctcca ggcgccggag cagcgggtag agcgtgttcg cgtccacgct gaagccgaga
     1741 tcgcgcagct ggtccagcag cgcgtagccg tagccgggct cccggagcag cagcaggctg
     1801 gcgagcacga ccgtgccgcg tcgcagctcc tgctgggttc ccgagaggat ctcgtcgaag
     1861 gtcatggcgg gcactatagt gtgcg
//
LOCUS       DBNHXO010000056         1984 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2793234, whole genome shotgun sequence.
ACCESSION   DBNHXO010000056 DBNHXO010000000
VERSION     DBNHXO010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2793234"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="AC162A_01265"
     CDS             complement(<1..239)
                     /locus_tag="AC162A_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR5009493.1"
                     /translation="MTGPLILAIESSCDETGVALVEDGRRIHANVVASQIALHAPTGG
                     IVPEVAARAHLRWIVPALDEALDTAGVTIPDVDAVA"
     gene            complement(236..802)
                     /gene="rimI"
                     /locus_tag="AC162A_01270"
     CDS             complement(236..802)
                     /gene="rimI"
                     /locus_tag="AC162A_01270"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR5009494.1"
                     /translation="MGIDDLASVQQIEHASFTTPWPPQAYRQEIESNRLAHYLVGELG
                     GDVVAYGGIWLMVDEAHVTTFAVHPAFRRRKIGERLLLAMLDLSAQRHATEATLEVRL
                     SNLAARRLYEKYGFRPVGIRPRYYSDNGEDALIMTTEPLASPAMVDRIAGLRVELDAA
                     PAPSPDPDPSFERTAPEPNPAPAGEGAA"
     gene            complement(829..1521)
                     /gene="tsaB"
                     /locus_tag="AC162A_01275"
     CDS             complement(829..1521)
                     /gene="tsaB"
                     /locus_tag="AC162A_01275"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR5009495.1"
                     /translation="MTAGGPLLVVDTATTTAVIALGSPSGSLIAERSWEAGYRHGEEL
                     LTRIEQLLRDEDVEAAALGGLVVGTGPGAFTGLRVGLATVKGLAYALNLPVVGVPTGE
                     ALLTAAGGADTANDLVLVQPAGPSDRVLTRPGEPSRILAGETDLELRPGERLVAVDLD
                     GREAPEAVARGRGARVGLAAALLDAGAARFAAGLPDDLARLVPEYVTLPRGVRDAPPG
                     DGGVAITGGTTP"
     gene            complement(1518..>1984)
                     /gene="tsaE"
                     /locus_tag="AC162A_01280"
     CDS             complement(1518..>1984)
                     /gene="tsaE"
                     /locus_tag="AC162A_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR5009496.1"
                     /translation="DAVCERVIATHDAEETRALAARLATVARPGDLLCLVGDLGAGKT
                     QFAKGFATGLGIEDTVSSPTVVLMTEYAGRLPMFHVDLYRLDTAADALAGGLLDERQL
                     AGVALVEWAERLGSALPRARLDVIIEGTGDEPRSIALRADDDGYCRYLEAAA"
BASE COUNT          259 a          726 c          753 g          246 t
ORIGIN      
        1 gcgaccgcat cgacgtcggg gatcgtgacc ccggcggtgt ccagcgcctc gtcgagcgcg
       61 ggcacgatcc agcgcaggtg ggcgcgagcc gcgacctcgg gcacgatgcc gccggtcggg
      121 gcgtggaggg cgatctggct ggcgacgacg ttggcgtgga tccgccgccc gtcctcgacg
      181 agcgccacgc ccgtctcgtc gcagctcgac tcgatcgcca ggatcagcgg gccggtcacg
      241 cggcgccctc gccggccggg gcgggattgg gctcgggcgc ggtccgctcg aaggacgggt
      301 cgggatccgg cgacggggcg ggcgcggcgt ccagctcgac tcgcagcccg gcgatgcggt
      361 cgaccatcgc gggcgaagcc agcggctcgg tcgtcatgat cagggcgtcc tcgccgttgt
      421 cgctgtagta gcgggggcgg atccccacgg gccggaagcc gtacttctcg tacaggcggc
      481 gcgccgccag gttcgacaac cggacctcga gggtcgcctc ggtcgcgtgg cgttgggccg
      541 acaggtcgag catcgcgagc agcagccgct ccccgatctt gcggcggcgg aacgccgggt
      601 ggacggcgaa ggtcgtgacg tgggcctcgt cgaccatcag ccagatgccg ccgtaggcga
      661 cgacgtcccc gccgagctcc ccgacgaggt agtgggcgag ccggttgctc tcgatctcct
      721 ggcggtacgc ctggggcggc cagggcgtgg tgaacgacgc gtgctcgatc tgctggaccg
      781 acgcgaggtc atcgatgccc atgggacgga tcgtgaggac gaccgtgttc atggcgtggt
      841 gccgccggtg atcgcgacgc ccccgtcccc gggcggggca tcgcggaccc cgcgaggcag
      901 cgtgacgtac tcggggacga ggcgggcgag gtcgtcgggc aggccggcag cgaaccgggc
      961 tgccccggcg tcgaggagcg ccgcggccag gccgacgcgg gcgccgcggc cccgggccac
     1021 ggcctccggc gcctcgcgcc cgtcgaggtc gaccgcgacc agccgctcgc cggggcgcaa
     1081 ctcgaggtcg gtctccccgg ccaggatccg cgacggctcg ccgggccggg tcaggacgcg
     1141 gtccgacggg ccggcgggct gcacgagaac gaggtcattg gcggtgtcgg cgccccctgc
     1201 cgcggtcagg agggcctcgc ccgtcgggac gcccaccacc ggcaggttca gcgcataggc
     1261 gaggcccttc acggtcgcca acccgacccg aagccccgtg aaggcgccgg gccccgtgcc
     1321 gaccacgagc ccgcccagcg cggctgcctc gacgtcctcg tcgcggagca gctgctcgat
     1381 cctcgtcagc agctcctcgc cgtgccggta cccggcctcc cacgaccgct cggcgatgag
     1441 cgagcccgag ggcgacccca gggcgatcac ggcggtcgtg gtggccgtgt ccacgaccag
     1501 caggggaccg ccggcggtca cgcggcggcc tccaggtacc ggcagtaccc gtcgtcgtcc
     1561 gcacgcaacg cgatcgaccg cggctcgtcg cccgtaccct cgatgatcac atcgagccgc
     1621 gcgcgcggca gcgcggagcc caaccgctcc gcccactcca cgagcgcgac gcccgcgagc
     1681 tgtcgctcgt ccagcagccc gcccgcgagc gcgtcggcgg cggtatcgag gcgatacagg
     1741 tcgacgtgga acatcggcag gcggcccgcg tactcggtca tcagcacgac tgtcggggag
     1801 ctgaccgtgt cctcgatccc gagccctgtg gcgaacccct tggcgaactg cgtcttgccg
     1861 gcgccgaggt cgcccacgag gcacaggagg tcgccggggc gggcgacggt cgcgagccgt
     1921 gcggccaggg cacgggtctc ctcggcgtcg tgcgtcgcga tgacgcgctc gcaaacggca
     1981 tctc
//
LOCUS       DBNHXO010000057         2376 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2594124, whole genome shotgun sequence.
ACCESSION   DBNHXO010000057 DBNHXO010000000
VERSION     DBNHXO010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2594124"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1295)
                     /locus_tag="AC162A_01285"
     CDS             complement(51..1295)
                     /locus_tag="AC162A_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015172532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR5009497.1"
                     /translation="MPNPTYDRSPWLLEPEISFLNHGSFGACPVPVLEAQRVWRDRLE
                     AEPVRFLDRELEPRLDEVRLEVARFLHADPEGIVFVDNATTGVATVLASIRFQPGDEL
                     LAGNHEYNATINALRAAADRDGATVTIVRVPFPIRDPSEAVEAYLEAVTPRTRFALVS
                     HVTSPTALVLPIEAIVRELDRREVEVLVDAAHAPGMVDVDLGRLAAPYWTANAHKWLC
                     APKGVAILHVRADRRKLVRPLVISHGRNSDRTDRSRFRLEFDWTGTADPSGILALPAA
                     LRYVGGLDEDGWPGFMATNRSLARRGRDVLCSALGVLPPAPDVMIGSMAAVPLPNLAP
                     TRAAAERLQVALFEEERIEVPITIFPVAAARDGGGEQALVRISAQRYNRPEEYARLAD
                     TLAKRLRGPSSPRALLGRLRRG"
     gene            complement(1340..1996)
                     /locus_tag="AC162A_01290"
     CDS             complement(1340..1996)
                     /locus_tag="AC162A_01290"
                     /inference="COORDINATES: protein motif:HMM:NF015534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HIR5009498.1"
                     /translation="MDALIGEARTVAGDRDPVVVIVPTAAARQRPDQAVEYGERAFQA
                     AAARAGIAVRLTTARILTRDDAADPSLIEPIGIADLVHFPGGDPDLIPAVHRDTPAWT
                     AILAALERGACVAGASAGAMALGGRVWTSRGGIDGLGLLPGTAVIPHFAPDRVTSWRR
                     IVEDGAALTWIGLDEQTLVLGRPGETWTVAGRGRMHLVPAGALEPTLVAHHGMAVSLG
                     "
     gene            complement(2053..>2376)
                     /locus_tag="AC162A_01295"
     CDS             complement(2053..>2376)
                     /locus_tag="AC162A_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009499.1"
                     /translation="LVALLPVWRLHVAGWPVRWLVTAWILYAIGILAVLRLPGLRLVI
                     PIVVLAYIAPFVAGPERVSRVLSGRRTTPAVVIDVTPRAAPGPPEAPRHVEGEVIDDD
                     PGRPS"
BASE COUNT          332 a          902 c          872 g          270 t
ORIGIN      
        1 ggcgacgacg tgcggatccg ggccaccgtc ccgcgcccct gaacgcggga tcagccccgg
       61 cgcaggcgcc cgaggagcgc gcgcggggag gagggcccgc gtaggcgctt ggcgagcgtg
      121 tcggccagtc gcgcgtactc ctcgggccgg ttgtagcgct gggccgagat ccgaacgagc
      181 gcctgctcgc cgccgccgtc ccgggccgcc gccaccggga agatcgtgat cgggacctcg
      241 atccgctcct cctcgaacag cgcgacctgg agtcgctcgg ccgcggcccg cgtcggggcc
      301 aggttcggga ggggcacggc ggccatcgac ccgatcatca cgtccggcgc cggcggcagg
      361 acaccgagcg ccgagcacag cacgtcgcgc ccgcgacgcg ccagcgaccg gttcgtggcc
      421 atgaagccgg gccagccgtc ctcgtccagg ccgcccacgt aacgcagggc ggcgggcagc
      481 gcgaggatcc cggatgggtc ggcggtgccg gtccagtcga actcgagcct gaaccgcgac
      541 cggtccgtcc ggtccgagtt ccggccatgc gagatcacca gcggccggac cagcttccgg
      601 cggtcggcac ggacatggag gattgccacg cccttcggcg cgcacagcca cttgtgcgcg
      661 ttggccgtcc agtacggcgc cgccagccgg ccgaggtcga cgtcgaccat gcccggcgcg
      721 tgggccgcgt ccacgagcac ctccacctcg cgccggtcga gctcgcggac gatggcctcg
      781 atcggcagca ccagcgccgt gggcgaggtg acgtggctca cgagcgcgaa ccgggtcctc
      841 ggggtcaccg cctcgagata cgcctcgacg gcctccgacg ggtcccggat cgggaacggg
      901 acgcggacga tggtgacggt cgcgccgtcg cgatcggcgg ccgcgcgcag ggcattgatc
      961 gtcgcgttgt actcgtggtt gccggccagc agctcgtcgc cgggctggaa gcggatcgac
     1021 gcgagcaccg tcgccacgcc ggtcgtcgcg ttgtccacga acacgatccc ctcggggtcg
     1081 gcgtggagga accgggcgac ctcgaggcgc acctcgtcga ggcggggctc gagctcacgg
     1141 tccaggaacc ggacgggctc ggcctcgagg cggtcgcgcc acacccgctg cgcctcgagc
     1201 accggcaccg ggcacgcccc gaacgagccg tggttgagga acgagatctc gggctcgagc
     1261 agccacggcg atcggtcgta cgtcgggttc ggcacgtcgc gacgatagcc gaccgccgga
     1321 caggcggtga cgcgctccgt cagcccagcg agaccgccat gccgtggtgg gcgacgagcg
     1381 tcggctcgag cgcgccggcc gggaccaggt gcatgcgccc gcgcccggcg accgtccagg
     1441 tctcgccggg ccgtccgagg accagcgtct gctcgtcgag cccgatccag gtcagcgcgg
     1501 caccgtcctc cacgatgcgt cgccaggacg tcacccggtc cggcgcgaag tgcgggataa
     1561 cggccgtgcc cggcagcagc cccagcccgt cgatcccgcc gcgcgacgtc cacacgcgac
     1621 cgcccagcgc catcgcgccg gcgctcgccc cggccacgca cgcgccccgt tcgagggcgg
     1681 cgaggatcgc agtccacgcc ggcgtgtcgc gatgcacggc cgggatgagg tccgggtcgc
     1741 ccccggggaa gtgcacgagg tcggcgatcc cgatcggctc gatcagcgac gggtccgcag
     1801 cgtcgtcacg ggtcaggatc cgcgccgtcg tgaggcgcac ggcgatgccg gcgcgggcag
     1861 cggccgcctg gaacgcccgc tcgccatact cgaccgcctg gtctggacgt tgacgcgcgg
     1921 ccgcggtcgg cacgatgacg accacgggat cgcggtcgcc cgcgacggtc cgcgcctcgc
     1981 cgatcagcgc atccatcgcc gcctcgtcgc cggccacgta ctcgccgccg ccgtgcaggg
     2041 cgatccgacc gctcaggacg gccgccccgg atcgtcgtcg atcacctcgc cctcgacgtg
     2101 gcgcggcgct tccgggggcc cgggcgccgc ccgcggggtc acgtcgatca cgacggccgg
     2161 cgtcgtcctg cgaccgctca gcacccgcga cacccgctcg ggtccggcga cgaacggggc
     2221 gatgtacgcc aggaccacga tcgggatcac gaggcgaagc cccggcagcc ggagcaccgc
     2281 gaggatgccg atcgcgtaca ggatccaggc ggtgaccagc cagcggaccg gccagcccgc
     2341 cacgtgcagc cgccacaccg gcagcagggc caccag
//
LOCUS       DBNHXO010000058         2838 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2991827, whole genome shotgun sequence.
ACCESSION   DBNHXO010000058 DBNHXO010000000
VERSION     DBNHXO010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2991827"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="AC162A_01300"
     CDS             complement(<1..917)
                     /locus_tag="AC162A_01300"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5009500.1"
                     /translation="MGVVGAGTMGAGIAQVALEAGDEVALYDVAPGAVELARTRIADG
                     LGRRAAKLGLDDGGAEDWVAVQLGRLRRVDTLDAVAEDALLVIEAAVEDLALKRVVFA
                     TLDARAAAGTILATNTSALPVASIAADASRRSRIVGLHFFNPAPVMALVEVVIGPDTD
                     ASTAARAEALVTRWGKTPIRCSDSPGFVVNRINRPFTLEPLRMLDLGAATIEEIDGAV
                     RAEGFPQGPFEHIDLIGLDVNLATSTAVHVGLGSPARLAPSALQARLVAARRLGRKTG
                     VGFYEYDASGHRVRPGPGFGRRGGKHVPL"
     gene            complement(954..1457)
                     /gene="paaD"
                     /locus_tag="AC162A_01305"
     CDS             complement(954..1457)
                     /gene="paaD"
                     /locus_tag="AC162A_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HIR5009501.1"
                     /translation="MTTAVGRALDRAMVVAALRDVPDPEIPVISVVDLGVIGRIEVDA
                     DHVRVELLPTFVGCPALEPMREAVRSRLVELAPGHEVAVAVVFDPPWTTERISPAGRH
                     RLAASGFAPPVAGGSAMPVALDVPVPCPWCGSRRTVLENAFGPTACRAIRYCTDCRQP
                     FEQFKPV"
     gene            complement(1454..2239)
                     /gene="paaC"
                     /locus_tag="AC162A_01310"
     CDS             complement(1454..2239)
                     /gene="paaC"
                     /locus_tag="AC162A_01310"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HIR5009502.1"
                     /translation="MTSDAHDLVPATRNALAILLLSMADDELVIGFGDSEWTGIAPLL
                     EEDVAMSSLAQDELGHALALYRLLADVAEDGRDADAIAYDRPPEGYFHARLLDHPRGD
                     WATAIARRYLYDTADGVRLAALEACTWAPLRDLAGKIRREERYHVMHAEAWLERLANG
                     DAESRRRLLDALDRLGADAWTVLAPLPNDLSLLMAGILDAPLTELDARWRGAIGPMFE
                     RLGLPMPAPAGDPAGARTGHSDAFRWLHGEFTSVRRLDPQATW"
     gene            complement(2236..2616)
                     /locus_tag="AC162A_01315"
     CDS             complement(2236..2616)
                     /locus_tag="AC162A_01315"
                     /inference="COORDINATES: protein motif:HMM:NF018002.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaB"
                     /protein_id="HIR5009503.1"
                     /translation="MTDQPPVDRLDEVGIEEPDVGLEPYEVFRQEKEGDAMRHGGSVM
                     APDRVLALHYARELYGRRQESVRLWVVRRADVAVLDDPDLLQPPLDRSFKKPGGFVMR
                     DKLAAARELSGQAKPHAKSKGGPG"
     gene            complement(2624..>2838)
                     /gene="paaA"
                     /locus_tag="AC162A_01320"
     CDS             complement(2624..>2838)
                     /gene="paaA"
                     /locus_tag="AC162A_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704148.1"
                     /note="with PaaBCDE catalyzes the hydroxylation of
                     phenylacetyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HIR5009504.1"
                     /translation="ELGLTLPDPDLRLDEAAGEWRYAEPDWDELRAVVTGHGPASAER
                     VAFRQLNWDEQAWVRTAIAATRPAAA"
BASE COUNT          388 a         1052 c         1039 g          359 t
ORIGIN      
        1 tcgagcggaa cgtgcttccc tccgcgtcgc ccgaaccccg gccccgggcg gacgcggtga
       61 cccgacgcgt cgtactcgta gaagcccacc ccggtcttcc gcccgagcct gcgcgccgcg
      121 acgagccgcg cctggagcgc ggacggcgcc agccgggccg gtgacccgag gccgacgtgc
      181 acggcggtcg acgttgcgag gttcacgtcg agcccgatga ggtcgatgtg ctcgaacggg
      241 ccctgcggga agccctcggc gcgcacggcc ccgtcgatct cctcgatcgt cgcggcgccg
      301 aggtcgagca tgcgcaacgg ctcgagcgtg aacgggcgat tgatgcggtt gacgacgaag
      361 cccggagagt cagagcagcg gatgggtgtc ttgccccacc gcgtcacgag cgcctccgcg
      421 cgcgccgccg tgctcgcgtc agtgtcgggt ccgatgacga cctcgacgag ggccatcacc
      481 ggggcggggt tgaagaagtg caggccgacg atccgcgagc gtcgcgacgc gtcggcggcg
      541 atcgaggcga ccggcagcgc gctcgtgttg gtggcgagga tcgtgcccgc cgcggcccgc
      601 gcgtcgagcg tcgcgaacac gacgcgcttg agcgcgaggt cctcgaccgc cgcttcgatg
      661 accagcaatg cgtcctcggc gaccgcatca agcgtatcga cgcgccgcag gcgtccgagc
      721 tgcaccgcca cccagtcctc ggcccccccg tcgtcgaggc cgagcttggc cgcccgccgg
      781 cccagtccgt ccgcgatccg ggtccgcgcg agctccacgg cgcctggcgc cacgtcgtac
      841 agcgcgacct cgtcgccggc ctcgagcgcg acctgggcga tccccgcgcc catcgtgccc
      901 gccccaacga cgcccaccct gaagggtgtg gtcgtcggcg gggcgtccac ggctcagacc
      961 ggcttgaact gctcgaacgg ctggcggcaa tccgtgcagt agcggatcgc ccggcaggcc
     1021 gtcggaccga aggcattctc gaggaccgtc cgtcgcgagc cgcaccacgg gcacgggacg
     1081 gggacgtcga gggcgaccgg catcgcggac ccgccggcga cgggtggggc gaacccactc
     1141 gcggcgaggc ggtggcgacc cgccggcgag atccgctctg tcgtccaggg cggatcgaac
     1201 accaccgcga cggccacctc gtggccgggt gcgagctcga ccagccgcga ccgcacagcc
     1261 tcgcgcatcg gttccagcgc ggggcagccg acgaaggtcg gcagcagctc gacgcgcacg
     1321 tgatccgcgt cgacctcgat ccgcccgatg acgccgagat ccaccaccga gatgacgggg
     1381 atctccgggt ccgggacgtc gcggagcgcg gccacgacca tcgcccggtc gagcgcgcgg
     1441 ccgacggcgg tcgtcaccac gtggcctggg ggtcgaggcg gcggacggac gtgaactccc
     1501 cgtgcagcca tcggaacgcg tccgagtggc cggtccgggc gcccgccggg tcgccggccg
     1561 gggcgggcat cgggaggccg aggcgctcga acatcgggcc gatcgcccct cgccagcgcg
     1621 cgtcgagctc cgtgaggggc gcgtcaagga tgccggccat cagcagcgac aggtcgttcg
     1681 ggagcggcgc gagcacggtc caggcgtccg cgcccagcct gtccagcgcg tcgagcagcc
     1741 ggcgccggga ctccgcgtcg ccgttcgcca gccgttccag ccaggcctcc gcatgcatga
     1801 cgtggtagcg ctcctcgcgc cggatcttcc ccgccaggtc gcgcagcggt gcccaggtgc
     1861 acgcctcgag ggccgcgagg cggaccccgt ccgcggtgtc gtacaggtag cggcgggcga
     1921 tcgcggtcgc ccagtcgccg cgcggatggt caaggagccg agcgtggaag tagccctcgg
     1981 gcgggcggtc gtaggcgatc gcgtcggcgt cgcggccatc ctcggcgaca tcggcgagca
     2041 gccggtacag cgccagggcg tgacccagct cgtcctgcgc gagcgagctc atcgccacgt
     2101 cctcctcgag cagcggcgcg atgccggtcc actccgagtc accgaagccg atcaccagct
     2161 cgtcgtcggc catcgagagc agcaggatgg cgagggcgtt ccgggtggcc gggacgaggt
     2221 cgtgcgcgtc cgacgtcatc cgggtccgcc cttggacttc gcgtgcggct tggcctgccc
     2281 ggacagctcg cgcgccgccg cgagcttgtc gcgcatcacg aagccgccgg gcttcttgaa
     2341 ggagcgatcg agcggcggct ggagcagatc agggtcgtcg agcacggcga cgtccgcgcg
     2401 ccgcacgacc cacaggcgga cgctctcctg gcggcggccg tagagctcgc gtgcgtagtg
     2461 gagcgccagc acccggtcgg gggccatgac cgacccgccg tggcgcatcg cgtccccctc
     2521 cttctcctga cgaaagacct cgtagggttc gagccccacg tccggctctt cgatccctac
     2581 ctcgtcgagc cggtcgaccg gcggctggtc cgtcatcggg atctcaggcg gccgccgggc
     2641 gggtagcggc gatcgccgtg cgcacccacg cctgctcgtc ccagttgagc tgccggaacg
     2701 cgacccgctc cgcggacgcc gggccatggc cggtgaccac cgcccgcagc tcgtcccagt
     2761 cgggctcggc gtagcgccat tcgccggccg cctcgtccag gcgcagatcc ggatcgggga
     2821 gcgtgaggcc gagctcga
//
LOCUS       DBNHXO010000059         2483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4381672, whole genome shotgun sequence.
ACCESSION   DBNHXO010000059 DBNHXO010000000
VERSION     DBNHXO010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4381672"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="AC162A_01325"
     CDS             complement(<1..803)
                     /locus_tag="AC162A_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009505.1"
                     /translation="MSNYRLPAAAAVLAAVTALACSTGSAPTTAPTSGPVGTPVGPAP
                     SPLIDGIAHPTGATDVVLRYDSAGGFVPVEFLAAHVPYFTLYGDGTVVFVQNSTETEP
                     PEDGIWRSSPLRTGKLSEEQVQDLLEFALRDGGLAIAKAEYQNPLVADAPTAIFEINA
                     DGGRKTVSVVALGMEGEPGPDSAIKASMAKLGERLRDFDRGGTLSSDAFTPAAYRAVL
                     MDATGGAGGPARPWPWPSIGLDDFKFPADPNALQQGTKILSPDELAAIGV"
     gene            1004..1318
                     /locus_tag="AC162A_01330"
     CDS             1004..1318
                     /locus_tag="AC162A_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HIR5009506.1"
                     /translation="MYEDRTLTCRDCGESFVFSSGEQTFFASKGLTNDPQRCPTCRAA
                     AKRARNASPGGSREYHAAICNSCGGQAVVPFQPRNDRPVYCSTCFDKVRAGVDESAPA
                     PA"
     gene            1448..1645
                     /locus_tag="AC162A_01335"
     CDS             1448..1645
                     /locus_tag="AC162A_01335"
                     /inference="COORDINATES: protein motif:HMM:NF018961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HIR5009507.1"
                     /translation="MIIIREMVGSSPRSWSDAARQAVATASKTVRNIRTIEVVKSTAR
                     IEGGEIVEYLVELKIGFEYEG"
     gene            1684..>2483
                     /locus_tag="AC162A_01340"
     CDS             1684..>2483
                     /locus_tag="AC162A_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5009508.1"
                     /translation="MPQTATGKHRDAPTRDGPAPELAETGNPTAKSRVDAGEPASVSF
                     AAKVGDGPGERAASNRSLGQGGNARVGVGPGIPGAMLLGGRAGILGMNPFPIVGARIH
                     PPLLRADTLTRERLNAWLDEAATGRVALVTAEAGFGKTTLLGDWARNSSRLTAWYRLD
                     PDDRDWLTFCRHLVASGRELDPEFAPETYALLMLLGPGGPTRADIQASLVDEFAAFGA
                     AHQQGLTLIFDDYHAVDGSDEVVPTVRALIERTGDGFSIVIASRSTPRL"
BASE COUNT          375 a          849 c          901 g          358 t
ORIGIN      
        1 acgccgatcg ccgcgagctc gtcgggcgac aggatcttcg tgccctgctg cagggcgttc
       61 gggtcggccg ggaacttgaa gtcgtcgagc ccgatcgacg gccacggcca cggacgtgcc
      121 ggaccgccgg cgccgccggt cgcgtccatc aggacggccc tgtaggccgc gggcgtgaag
      181 gcgtcgctcg agagcgtgcc gccgcggtcg aagtcgcgca ggcgctcacc gagcttggcc
      241 atcgaagcct tgatcgcgct gtcggggccg ggctcgccct ccatgccgag ggcgacgacc
      301 gagacggtct tcctgccgcc gtcggcgttg atctcgaaga tcgccgtcgg ggcgtcggcg
      361 accagcgggt tctggtactc ggccttcgcg atggcgagcc caccgtcacg gagcgcgaac
      421 tcgaggaggt cctggacctg ctcctcgctc agcttgccgg tgcgcagcgg ggagctgcgc
      481 cagatgccgt cctcgggcgg ctccgtttcc gtcgagttct ggacgaacac gacggtcccg
      541 tcgccgtaca gcgtgaagta gggcacatgc gcggcgagga actcgaccgg gacgaaccct
      601 ccggcggagt cgtaccggag cacgacgtcg gtcgctccgg tcgggtgcgc gatcccgtcg
      661 atgaggggtg acggggcggg tccgaccggg gtccccacgg gaccggatgt cggcgcggtg
      721 gtgggagctg aaccggtcga gcaggcgagg gcggtgacgg cggccagaac ggcggcggcg
      781 gcgggcaggc ggtagttcga catgggccat cggacgcgcg gaacgtcggc gaggttcccg
      841 aacgtgggta aatcctattg acgacgcgcg tatcctccgc gcaaagaagt tccgggctcg
      901 cgagcccgcc tgcccgagcg ccgaccgtta cagacgacgc cgagccggcc cgacgccggg
      961 tcttgagctt gcatatcggt ggtttgaggg aggaccccga ggggtgtacg aggaccgcac
     1021 gttgacttgc cgtgactgcg gcgagtcgtt cgtcttctcg tcgggcgagc agacgttctt
     1081 cgccagcaag ggcctgacca acgatccgca gcggtgcccg acctgcaggg ccgcagcgaa
     1141 gcgggccagg aacgcgagtc cggggggttc tcgcgagtac cacgcggcca tctgcaacag
     1201 ctgcggcggg caggcggtgg tgccgttcca gcccaggaac gatcgcccgg tctactgcag
     1261 cacctgcttc gacaaggtcc gagccggcgt ggacgagagc gcgccagcgc ccgcctgatc
     1321 cgcaagcgtc gtcgcgtccc cacgccccac ggcctcttcg gtcgcgcctc cgtcacgcgg
     1381 cgcggtcacg gtcccggagg tccggcctat gctgggctgg tccgatgcta ggaggagccc
     1441 agtgggcgtg atcatcatcc gagagatggt cgggtcgagt ccccgatcgt ggagcgacgc
     1501 cgctcgccag gccgtggcca cggcatccaa gaccgtgcgc aacatccgca ccatcgaggt
     1561 cgtgaagtcg acggcccgaa tcgagggtgg cgagatcgtc gagtacctcg tcgagctcaa
     1621 gatcggcttc gaatacgagg gctgataagc cgccgatgag cggcgctcgg caagatgccg
     1681 ggtatgcccc agaccgccac cggcaagcac cgagacgcac cgacacgcga cggtccggcg
     1741 cccgagctcg ccgagaccgg caatccgacc gcgaaatccc gcgttgacgc gggcgaaccg
     1801 gcgtccgtat cgttcgccgc caaggtcggc gacgggccag gggagagggc agcgtccaac
     1861 cggtccctgg ggcaaggggg caatgcgcgt gtcggcgtcg gaccgggcat cccgggcgcg
     1921 atgttgctcg gcggtcgtgc gggcatcctc ggcatgaacc cgttcccgat cgtcggcgcc
     1981 cggatccatc cgccgctcct gcgcgcggac acgctcacgc gcgaacgcct gaacgcctgg
     2041 ctcgacgagg cggcgacggg tcgcgttgcg ctggtcactg cggaggccgg cttcggcaag
     2101 accacgctgc tcggcgattg ggcgcgcaac agctcgcgac tgaccgcctg gtaccgcctc
     2161 gatcccgacg atcgcgactg gctcacgttc tgccgccacc ttgtcgcgag cggccgtgag
     2221 ctcgaccccg agttcgcccc cgagacgtat gcgctgctga tgctgctggg ccccggcggg
     2281 ccgacccgcg ccgacatcca ggcgagcctc gtggacgagt tcgcggcctt cggcgccgcg
     2341 catcagcagg gcctgacgct gatcttcgac gactaccacg cggtcgacgg cagcgacgag
     2401 gtcgtcccga ccgtccgcgc cctgatcgaa cgcacgggag acgggttcag catcgtgatc
     2461 gcgtcgcgct cgacgccgcg gct
//
LOCUS       DBNHXO010000060         3014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4977197, whole genome shotgun sequence.
ACCESSION   DBNHXO010000060 DBNHXO010000000
VERSION     DBNHXO010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4977197"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="AC162A_01345"
     CDS             <1..626
                     /locus_tag="AC162A_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017625789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR5009509.1"
                     /translation="PLPGRIAAAGIPMVVSPRPRRPMSVSYVDVDNRGGANSAVAHLV
                     AAGRRVIATVAGPLDMAPGVDRLAGYRDALLDAGLGSDPSLEVVADFTQEGGAKAMER
                     LLSARPDIDAVFAASDLIAAGALSVLAAAGRRVPEDVAVVGFDDSPVASSTRPALTSV
                     RQPIEEMGHEMARLLIDSIEGADRAPRRVILATELIRRASSAGRRVP"
     gene            742..2277
                     /locus_tag="AC162A_01350"
     CDS             742..2277
                     /locus_tag="AC162A_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 periplasmic-binding domain-containing
                     protein"
                     /protein_id="HIR5009510.1"
                     /translation="MFKSRSGPRALVALAGTVAIVLTACTSSTATPAPSAAAPSQAAA
                     VTPAPSATPEPTPLLTAAPVDASVPGPNGGQIVRWFVGLGAGGQPQQIQVQTDFVTKF
                     NNDPANKDKQYISLEIYDNKVAANILKTQIAAGNAPDIIGPVGVEGLNLFIDQVADLQ
                     PQIDSTGYDMSKYPAALANFFKIGKDGATIGVPFGTYPSFLWYNKKLFDEANLPYPPT
                     KVGDLYEGKPWDMAAVRELGMKLTVDANGNDATSPDFDPTNIVQWGFDSQWADNAPTA
                     ETALFGAGSFLAADGKSAQIPDPMRTGIHWFNDGVWKDHFIPTQNQVNSDLLAKGNPF
                     QSGNLAMTEIHTWYSCCVNPAAPAKPIVGDNFGWAVAPSFNGATTAKLHADTFSMLKT
                     TKHPDEAFAVLSALAASGELATIYGAMPADPAQTQPFKDAIEAAYPKADLDWAVPEAM
                     LGNPDIPNHQAWVPDYAKSKAAWQAFGVGYRTAESADIDADLDKLKDTLQGIFDAAPD
                     KNP"
     gene            2386..>3014
                     /locus_tag="AC162A_01355"
     CDS             2386..>3014
                     /locus_tag="AC162A_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5009511.1"
                     /translation="MSATIEELPTTPAPSRRSFVLRPSVREALWGFVFIGPWLIGLVL
                     FIAGPMIASLVMSLTDFNLVRPETTKFVGLDNYVRMANDPTISQSLIATLKFALIAIP
                     VTMLASLGFALLLNHPKLPAKGPLRALVYLPVMIPLVASTLVWIGFLNTETGWLNQIL
                     GSLGLPQPDWINSETWIYPTLSIIGLWAIGNFMIINIAGLQSVPTELYEA"
BASE COUNT          502 a         1146 c          923 g          443 t
ORIGIN      
        1 accccctgcc cggccgcatc gcggcggcgg gcatcccgat ggtggtctcg ccgcgcccgc
       61 ggcggccgat gtcggtcagc tatgtcgacg tcgacaaccg cggtggcgcc aacagcgccg
      121 tcgcccacct cgttgccgcc ggtcggcggg tcatcgccac ggtcgccggt cctctggaca
      181 tggcacccgg cgtggaccgg ctcgccggct accgcgacgc cctgctggac gccggcctcg
      241 gctccgaccc cagcctggag gtcgtcgcgg acttcaccca ggagggtggc gccaaggcga
      301 tggagcggct gctttccgct cgccccgaca tcgacgccgt attcgccgcc tccgacctga
      361 tcgcggcggg cgcgttgtct gtactggccg ccgcaggtcg ccgcgttccc gaggacgtcg
      421 ccgtcgtcgg gttcgacgac tcgccggtcg cgagcagcac gcgtccggcg ctcaccagcg
      481 tccgccagcc gatcgaggag atgggccacg agatggcccg gctgctcatc gattccatcg
      541 agggggcgga tcgcgcgcca cggcgcgtga tcctcgccac cgaactcatc cgtcgggcct
      601 cgagcgccgg gaggcgcgtg ccctgagccg gccggcgacc ggcgggcgac ggccttggga
      661 gaccgtcacc ccacccactt aggcctcttt catcgaggta ggagatccga gaagggtctc
      721 gaacaggagg agtcgatcgc gatgttcaag tctcgatccg gtccgcgggc cctcgtggcg
      781 ctcgcgggca cggtcgccat cgtgctgacg gcatgcacga gctcgaccgc gaccccagcg
      841 cccagtgccg ccgcgccgag ccaggccgcc gccgtcacac cggccccgag cgcgacaccc
      901 gagcccacgc cgctcctgac ggccgcgccc gtcgacgcca gcgtgcccgg ccccaacggc
      961 ggccagatcg tccgctggtt cgtcggcctg ggcgcgggtg gtcagccgca acagatccag
     1021 gtccagaccg acttcgtcac caagttcaac aacgaccccg cgaacaagga caagcagtac
     1081 atctcgctcg agatctacga caacaaggtc gcggccaaca tcctcaagac gcagatcgcc
     1141 gccggcaacg cgccggacat catcggcccg gtcggcgtcg aggggctgaa cctgttcatc
     1201 gaccaggtgg ccgacctgca gccgcagatc gactcgaccg ggtacgacat gagcaagtac
     1261 ccggccgccc tggccaactt cttcaagatc ggcaaggacg gcgccacgat cggcgtgccg
     1321 ttcgggacct acccgtcctt cctctggtac aacaagaagc tgttcgacga ggccaacctg
     1381 ccgtacccgc cgaccaaggt cggtgacctg tacgaaggca agccgtggga catggccgcc
     1441 gttcgcgagc tgggcatgaa gctcaccgtc gatgccaacg gcaacgacgc cacgagcccg
     1501 gacttcgatc ccaccaacat cgtccagtgg ggcttcgact cccagtgggc agacaacgcc
     1561 ccgacggccg agacggccct gttcggcgcc ggctcgttcc tcgccgccga cggcaagtcg
     1621 gcccagatcc ccgacccgat gcggaccggt atccactggt tcaacgacgg cgtctggaag
     1681 gaccacttca tcccgaccca gaaccaggtc aacagcgacc tgctggccaa gggcaacccg
     1741 ttccagtcgg gcaacctcgc catgaccgag atccacacgt ggtactcgtg ctgcgtgaac
     1801 ccggcggccc cggccaagcc catcgtcggt gacaacttcg gctgggccgt cgccccgtcg
     1861 ttcaacggcg ccaccacggc caagctgcac gccgacacgt tcagcatgct caagaccacc
     1921 aagcacccgg atgaggcatt cgccgtcctg tccgccctcg cggcgtccgg cgagctcgcg
     1981 accatctacg gcgcgatgcc cgccgacccg gcccagacgc agccgttcaa ggacgccatc
     2041 gaagcggcct acccgaaggc cgatctcgac tgggccgtcc cggaggccat gctcggcaac
     2101 ccggacatcc ccaaccatca ggcctgggtc ccggactacg ccaagagcaa ggccgcctgg
     2161 caggccttcg gcgtcggcta ccgcaccgcc gagagcgcgg acatcgacgc cgacctcgac
     2221 aagctcaagg acaccctcca gggcatcttc gacgctgccc cggacaagaa cccctagcta
     2281 acccctcctc cagcggcctc ccggagcggt gccccgaccc tccgggaggc cgatttctcc
     2341 cccatcgatt ccgatcacgg acccgcacgg agcccggacc cgacgatgag cgcgacgatc
     2401 gaggaactgc ccacgacgcc cgcgccctcc cgtcgctcgt tcgtgctccg tccgtccgtc
     2461 cgcgaggcgc tgtggggctt cgtgttcatc ggaccgtggc tgatcggcct cgtgctgttc
     2521 atcgccggtc caatgatcgc gtcgctcgtg atgtcgctga ccgacttcaa cctggtccgt
     2581 cccgagacga ccaagttcgt cggcctcgac aactacgtcc gcatggcgaa cgacccgacg
     2641 atctcgcagt ccctgatcgc gaccctcaag ttcgcgctga tcgcgatccc agtgacgatg
     2701 ctcgccagcc tcgggttcgc cctgctcctc aaccacccca agctgccggc caagggtccg
     2761 ttgcgggcgc tcgtgtacct gcccgtgatg atcccgctgg tcgcgtccac gctcgtgtgg
     2821 atcggcttcc tcaacaccga gacggggtgg ctcaaccaga tcctcgggtc gctcgggctg
     2881 ccacagccgg actggatcaa cagcgagacc tggatctacc cgacgctctc gatcatcggc
     2941 ctgtgggcga tcggcaactt catgatcatc aacatcgccg ggctccagtc ggtgcccacc
     3001 gagctgtacg aggc
//
LOCUS       DBNHXO010000061         6145 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4778699, whole genome shotgun sequence.
ACCESSION   DBNHXO010000061 DBNHXO010000000
VERSION     DBNHXO010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4778699"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1271)
                     /locus_tag="AC162A_01360"
     CDS             complement(192..1271)
                     /locus_tag="AC162A_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HIR5009512.1"
                     /translation="MPTITERAVFDALRDVQEPELGRDIVTLDMVKDVVIDGSAVAFT
                     IELTTPACPLKDEIEGNARAVLGGIGVDRIDITWGAMVRRAQPRQAEQLLPGVKNVLA
                     VASGKGGVGKSTVSVNLAVALAQAGASVGLLDADITGPNIPMMLGLDGQPKATADNKI
                     APLERHGVKAISIQFFVPEGQPIVWRGPLVGGAIQQFLRDVDWGELDYLVIDLPPGTS
                     DAQLTLAQAVPISGAVLVTTPQEVALSDVAKALAMFKRMSVPVLGLVENMTAFVCPHC
                     GEVTEIFGRGGGERFAKEHGIDYLGGVPLDVTVRQGGDVGVPAVAQREPGPAALALTA
                     VAGAVAARMSVRAAQAADRPILTVS"
     gene            1544..2647
                     /locus_tag="AC162A_01365"
                     /pseudo
     CDS             1544..2647
                     /locus_tag="AC162A_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492018.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3068..4018
                     /locus_tag="AC162A_01370"
                     /pseudo
     CDS             3068..4018
                     /locus_tag="AC162A_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
     gene            4053..5093
                     /locus_tag="AC162A_01375"
     CDS             4053..5093
                     /locus_tag="AC162A_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005311286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009513.1"
                     /translation="MTRHRWLIPYLLLLPGLLWLAIFFVLPNIQMFLMSLSTKGAGSG
                     LTQQYEFTWAFSNYADALTDFPDQFANSLIYGGLATLFCLLIGYPLAYGIAFRGGRYK
                     NLLLFLVIAPFFTSFLIRTISWKIILGNDGAFLSIIRDVLGIVPDNFSVLGTPLAVVS
                     GLTYQFLPFMVLPLYVSLEKIDRRLIEAAQDLYAGPQRQGGALIGGIAGAVLAVGVMA
                     GLSYLPLSLDRALNIAVVAAIGALIGATIATLLISESFTKVVFPLSLSGIFAGVILTF
                     IPAIGDYVNAELLGNPKTQMIGNVIQNRFLAQNDYPTAAALSFLLMAGILIAIFAYAR
                     ALGSDELTGGAG"
     gene            5090..6037
                     /locus_tag="AC162A_01380"
     CDS             5090..6037
                     /locus_tag="AC162A_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013731699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009514.1"
                     /translation="MSSAPTTMQQTATGGVGTVKRATKFLDRWLVPVFVAFALFYLIL
                     PILVMIAFSFNDPPGRFNFVWGQFSMAAWENPFGRPGLQAAIVTSLVVATVATLVATI
                     LGTLIALALSRYRFRGQMGTSLFIFIPMATPEIVLGASLLTLFVGSANEPWKTLTGGA
                     LFPLGIQTVLIAHIMFCISFVVVTVRARMQGFPRHLEEAAMDLGASPWATFWKVTFPL
                     ILPGIMAGALLAFSLSIDDFIVTNFTSGTASTFPIWVWASIKNNLPPQVHVIGTMVFV
                     GAVGLVALSTLWERRNERRDQQIAAREAAKAAATYGVTD"
BASE COUNT          938 a         2210 c         2006 g          991 t
ORIGIN      
        1 cgacgccgca tcggtcgcag aaccgggcgc cgatggccgc gtcggcgccg caggcgtcgc
       61 atcgcactgg cggacgactt cctctcccat tggcgcgctc gacggcgcgc tcggtcgcga
      121 cagtctaggg accgggagcc ggtccggata ggcgccgcct ccgccccgcg ggcgacgggc
      181 agcgccgccc gtcaggagac ggtcaggatc ggccggtccg cggcctgggc cgcgcgaacg
      241 ctcatccggg ccgcgacggc gccggccacg gcggtcaggg ccagcgccgc cggaccgggc
      301 tcacgctggg cgaccgccgg gacgccgacg tcgccaccct gccggacggt gacgtcgagc
      361 gggacgccac cgaggtagtc gatgccgtgc tccttggcga accgctcgcc cccgcctcga
      421 ccgaagatct cggtgacctc gccacagtgc gggcagacga aggccgtcat gttctcgacc
      481 agcccgagga ccggcacgct catccgcttg aacatcgcga gggccttggc gacatccgag
      541 agcgccacct cctgcggcgt cgtcaccagg acggccccgg agatcggcac cgcctgtgcg
      601 agggtcaact gcgcgtcgga ggtgcccggc ggcagatcga tgacgaggta gtcgagttcg
      661 ccccaatcca cgtcgcggag gaactgctgg atcgccccgc ccacgagcgg gccgcgccag
      721 acgatcggct ggccctcggg gacgaagaac tggatcgaga tggccttgac gccgtggcgt
      781 tcgagcggcg cgatcttgtt gtccgcggtg gccttcggct gcccgtcgag gccgagcatc
      841 atcgggatgt tcgggccagt gatgtccgcg tccagcaacc cgaccgaggc accggcctgg
      901 gcgagggcga ccgcgagatt cacgctgacc gtgctcttgc cgaccccgcc cttgcccgac
      961 gccaccgcga gcacgttctt gaccccgggc aggagctgct cggcctggcg gggctgcgcg
     1021 cggcggacca tcgctcccca ggtgatgtcg atgcgatcca ccccgatccc gccgaggacg
     1081 gcccgagcgt tgccctcgat ctcgtccttg agcgggcagg ccggggtcgt gagttcgatc
     1141 gtgaacgcga cggccgatcc atcgatcacc acgtccttga ccatgtcgag ggtgacgatg
     1201 tcgcgtccga gctcgggttc ctggacgtcg cgcagggcat cgaagacggc gcgctcggtg
     1261 atggtgggca tggatcgtcg gtcccttcgc ggtgtcggcc cggtgttctg acggatggag
     1321 cgtcgatatc ctaccgcgcc atccgtccac gggcctcgat gggctcggcg ggcggtcgcg
     1381 ggcacgtccg gacgtggggg tctcgcccga cgaatgtcgc agcgaagatg acgacgcgct
     1441 gcgaatctcg cacttgtcgg accggtcgac gacgtcgatt tcttgtttga catgcaccac
     1501 tggcgaccat atcctcgggc cgacccgccc cttcccccaa ggagtgccaa cgaccgtgcc
     1561 caccgtcgac gtcaggctcg tggacgtggt caagaagtac ggcgagaacc tcgtcgtcga
     1621 ccacatcaac ctggaagtcc agtcggggga gttcttcacc ttgctcgggc cctcgggctg
     1681 cggcaagacc accaccctgc ggatgatcgg cgggttcgag gcgcccacgt ccgggctcat
     1741 cgagctcaag ggcgaggacg tcacgtggtt gccgccctac aagcggcacg tcaacacggt
     1801 cttccagaac tacgccctct tcccccacct gacgatctac gagaacgtcg cgttcggcct
     1861 ccgccgttcg aaggtcgacg acgccgaggt caagacccgc gtcaccgata tgctcaagct
     1921 cgtcgagctg cccgggttcg aggggcgcaa gccgacccag atctccggcg gccaggcgca
     1981 gcgcgtggcg ctcgcccgtg ccctcatcaa ccggccctcg gtgctgctcc tcgacgagcc
     2041 gctcggtgca ctcgatctca agctgcgcaa gcagatgcag gtcgagctca agcggatcca
     2101 gcaggaggtc gggatcacgt tcatcttcgt gacccacgac caggaagaag cgatgacgat
     2161 gtccgatcgc atcgccgtca tgaacaaggg caagtacgag cagctcgccg atcccgcgag
     2221 cctgtacgag cgacctcgga cgcggttcgt cgccgggttc ctcggcgtga gcaacctcct
     2281 cgcgaccacg cacgacggct cggtcgatgg cgcctactcg gtgtgcaagc tggccgacgg
     2341 cacgatcatc cgtgtcccgt cgtcgctcac cgagggcatc gagaagtacg aggtcggggt
     2401 gcggcccgag aagatccgcc tccgcgagcc cgcggaggac gtacccgccg gcttcaacca
     2461 gctgcacggg gtcgtccgcg acgcgtcgta tttgggcgtc tcgaccagct acgtcgtcga
     2521 gacccgtggc ggcggcacgg tcaccgtcta cgagcagaac gtcgagcgcg ccacgcgcgc
     2581 ggagctgtgg gagcccggcg aagaggtcgt cctgacctgg tcgcctgacc acacgttcgc
     2641 cgtcgagggt ggcggcgcgc cgccgcccga cgtcgtcacg gcggccgtcg tcggcgctgc
     2701 gggtgccgcg gccgcggtcc cgccgcctcg gagtacctcc cggcgcgcgt tcctgatcgg
     2761 cggggcggtc gtcgccgtcg gtggcgtcgc cgcgttcatc gccgccacgt caggcggcag
     2821 caagccgccg gccgcgaccc ccgcgccatc gaccgctctc gggccgacgc ccgcgcccgt
     2881 gccgaccgcg gccgccaccg ccgagggcag cgccagcgcg cccgttgccc agaccccgac
     2941 cggtccgctc aacttcgcca actgggacgc ctacatcgac ctcacgacgg tgcccgggcc
     3001 tgacggcgag ctgggcacgg acgacgacga gtacgacctc ccgtcgccga ccctggacga
     3061 gttcgccgcc aagtacgcgg tggaggtcaa ctaccagaac gcggtcgacg agaacgagtc
     3121 gttcatgggg acgatcgcgc cccagctgtc cgcgggcctc gacaccggct gggacctgat
     3181 cgtccttacc gactggatgg cgtctcgcgt cgtggcggcc gggtgggccg agaagatcga
     3241 ccacaacaac acgccgactg ccgtcgccaa cgtccgcgac gagctcgccg ggttcccctg
     3301 ggatccgaac ttcgacttcc actttccgtg gcagtcgttc tcgaccgggg tgggcttcaa
     3361 cgtcgggtcg acacagcggg agctcaccaa gatcgccgac ctgttcgacc cggcgttcgc
     3421 gggcaaggtc acgctcctct cggacccgca cgactcgttc cccctggtcc acctcatgct
     3481 ccaggcccag ggcaaggcat cggcgacgcc ggccgaggcg atgaccgtcg aggacggcca
     3541 ggtcgtccac gacttcctca agccctacgt ggacagcgcg cacatccgca agttcaccgg
     3601 caacgcctac ctgcaggact tcgccagcgg cgacacctgg gcggccttcg tctggtccgg
     3661 cgacctcgcc tcgtcgggcg gggacgacga ccggttcgtg taccccgaag agggctcgtt
     3721 gatcgcgacc gacaacatga tcatccccaa gggtgcgcag cataagtaca ccgccgagct
     3781 gatgatcgac tgggtctacg acgtcgaccg ggccgcccgg atcgccgcgt tcgtgtacta
     3841 cgtctcaccg gtcaagggcg ttgccgaggc gatcaaggcg ctcgaccccg acgccgccac
     3901 caatcccctg ctcttcccgc cccccgaggt gctcgcgaag cagcatcccc agccgatctg
     3961 ggacgagccg accgccgcaa agatgaacga gctgtacgcc gacctgagcg gcgtctagcg
     4021 cagcccagcc gacctgtgag gactcgcgaa gcgtgacccg ccaccgctgg ctgatcccat
     4081 acctgctgct gttgccgggc ctcctgtggc tcgcgatctt cttcgtcctg cccaacatcc
     4141 agatgttcct gatgtcgctt tcgacgaagg gggcgggcag cggactgacc cagcagtacg
     4201 agttcacctg ggccttcagc aactacgccg acgcgctgac cgacttcccc gatcagttcg
     4261 cgaactcgct catctacggc gggctggcga cgctgttctg cctcctcatc ggctacccgc
     4321 tcgcctacgg gatcgcgttc cgaggcggcc gctacaagaa cctgttgctg ttcctggtca
     4381 tcgcgccgtt cttcacgagc ttcctgatcc gcacgatctc gtggaagatc atcctgggca
     4441 acgacggggc gttcctctcg atcatccgcg acgtgctggg catcgtgccc gacaacttca
     4501 gcgtcctcgg gacgccgctc gcggtcgtgt ccgggctgac gtaccagttc ctgccattca
     4561 tggtcctgcc gctgtacgtg tcgctggaga agatcgaccg acggctgatc gaggccgcgc
     4621 aggacctgta tgcaggcccg caacgccagg gcggcgcgct catcggcggg atcgcgggtg
     4681 ccgtgctggc ggtgggggtg atggccgggc tcagctacct gcccctgagc cttgaccgcg
     4741 ccctcaacat cgccgtcgtc gccgccatcg gcgccctgat cggcgccacg atcgcgaccc
     4801 tgctgatctc ggagtcgttc accaaagtcg tgttcccgct cagcctgtcg ggcatcttcg
     4861 cgggggtgat cctcaccttc atccctgcca tcggcgacta cgtcaacgcc gagctcctgg
     4921 gcaacccgaa gacgcagatg atcgggaacg tgatccagaa ccggtttctc gcccagaacg
     4981 actacccgac cgcggctgcc ctctcgttcc tgctgatggc cggcatcctg atcgcgatct
     5041 tcgcctacgc gagggcgctc ggcagcgacg agctcacagg gggtgcggga tgagctcggc
     5101 tccgaccacg atgcagcaga ccgcgactgg cggcgtcggg actgtcaagc gcgcgaccaa
     5161 gttcctcgac cgctggctcg tcccggtctt cgtcgccttc gcgctgttct acctgatcct
     5221 gccgatcctc gtgatgatcg cgttctcgtt caacgacccg cccggcaggt tcaacttcgt
     5281 gtggggccag ttctccatgg cggcctggga gaacccgttc ggacggcccg gcctgcaggc
     5341 ggcgatcgtc acgagcctcg tcgttgccac ggtcgcgacg ctcgtcgcca cgatcctcgg
     5401 cacactgatc gcgctcgcgc tgtcgcgcta caggttccgt ggccagatgg gcacgagcct
     5461 gttcatcttc atcccgatgg cgaccccgga gatcgtgctc ggcgccagcc tcctgacgtt
     5521 gttcgtgggc tcggcgaacg agccctggaa gacgctcacc ggcggggcgc tgttcccgct
     5581 cgggatccag acggtcctga tcgcccacat catgttctgc atcagcttcg tggtcgtgac
     5641 ggtgcgtgcc cggatgcagg ggttcccgcg tcacctcgag gaggcggcga tggacctggg
     5701 cgcctcgccg tgggcgacct tctggaaggt gaccttcccg ctgatcctgc cgggcatcat
     5761 ggccggcgcc ctgctcgcgt tctcgctctc gatcgacgac ttcatcgtga ccaacttcac
     5821 ctcgggcacc gcgtcgacct tcccgatctg ggtctgggcg agcatcaaga acaacctgcc
     5881 gccgcaggtg cacgtgatcg gcacgatggt cttcgttggc gccgtgggcc tcgtcgccct
     5941 gagcacgctg tgggagcggc gcaacgagcg tcgcgaccag cagatcgcgg cccgcgaggc
     6001 ggccaaggcc gccgcgacct acggcgtcac ggactgagca cgaggacgac cccgccagcc
     6061 gcgcaaggga gaagccgatg accgccatct ccgacacgga cgcccgcccg ggcgcggctg
     6121 ccactcccct gcccgatcgc tccgt
//
LOCUS       DBNHXO010000062         9981 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1406237, whole genome shotgun sequence.
ACCESSION   DBNHXO010000062 DBNHXO010000000
VERSION     DBNHXO010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 9981)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9981)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9981
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1406237"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="AC162A_01385"
     CDS             complement(<1..399)
                     /locus_tag="AC162A_01385"
                     /inference="COORDINATES: protein motif:HMM:NF021465.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone"
                     /protein_id="HIR5009515.1"
                     /translation="MFAVGHLRDPRRDRAILAGSLVALSGLAWLTLWVWAASPYGRYL
                     HHDGGIAPLPVEAALFSLGWLLMIVAMMLPSSVPLVVTFGAIVGRRPQPRLLVALLVA
                     GYLAIWGAFGLAAWILDRGIHAAVEASPWLA"
     gene            complement(414..1022)
                     /locus_tag="AC162A_01390"
     CDS             complement(414..1022)
                     /locus_tag="AC162A_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HIR5009516.1"
                     /translation="MAYDIEGRLLEVCTCNVLCPCWVGEDPDGGTCDSALAWHIDSGS
                     IEGVDVTGRTVALSVHIPGNVLVPASWKAAVFVDDGASDEQQGELLKVFTGQLGGAIA
                     DLAGLIGEVVSVERVPITFTVDGGEGRLRIGEVADADLIPFTGATGKTTVLSETVFST
                     IPGSPVYAAKSHHFTRNGKPHGLPDVNRKGNNALQGHFRFAA"
     gene            1178..1858
                     /locus_tag="AC162A_01395"
     CDS             1178..1858
                     /locus_tag="AC162A_01395"
                     /inference="COORDINATES: protein motif:HMM:NF022589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HIR5009517.1"
                     /translation="MNELWTALVPLVIGSAILPVQITVTVLLLRSGAGRIAAVAWLAG
                     MTLVRLAQGIIFGFVFGVGVADAQGPARPGPIASTMLLVVGVLFLVSAVRKLLDDPDD
                     DAPPPTWMAMMSTATPGRAFGFGAGVVGASPKLWAFTLGAIAVIAEADLGQPAAAGMF
                     LVFVVLAESIHLALVAFAYAAPRRAEPALDRVTELLARYNRPLMIGLGLVFGVWFLAK
                     ALDGFGVL"
     gene            2109..3518
                     /locus_tag="AC162A_01400"
     CDS             2109..3518
                     /locus_tag="AC162A_01400"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HIR5009518.1"
                     /translation="MTTPTVDPGDVAPAVAALDTIVSLSKRRGFIFPSSEIYGGINAV
                     WDYGPLGVELKNNVKRAWWRAMVQDRDDIVGLDAGILMHPQVWVTSGHVGSFSDPLVE
                     CATCHRRYRLDELPGAEDLSATDLRDETIVSRLGLVCPNDAGPLSAPRRFNLMFTSHM
                     GPVEDDASIVYFRPETAQGSYVNFKNVQQSSRKKIPFGIAQIGKSFRNEISPGNFVFR
                     MREFEQMEMQYFVRPDTGASEAFEYWLPRRRAWYEAYGVTPSRLRFREHAPDELAHYA
                     KKAIDVEYRFPFGWKELEGIHNRGDFDLGNHAKASGENLEYFDQATEEHFIPWIVETA
                     AGADRAAFTFLIDAYREEEVRGETRVVLGLHPDLAPYKVAVLPLLKKRPEIVEMCHRL
                     LADLKRDLMAVYDDTAAIGKLYRRQDEIGTPWCVTVDVDSLEDGAVTIRDRDTMGQER
                     VPLEGVKRLLLDRMAAVRP"
     gene            3540..4421
                     /locus_tag="AC162A_01405"
     CDS             3540..4421
                     /locus_tag="AC162A_01405"
                     /inference="COORDINATES: protein motif:HMM:NF021378.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR5009519.1"
                     /translation="MTRATREDPRLRDVLTLAIAVAGLAACITLIYLAMRAVMDVGGA
                     CADGGPYVSAQPCPDGTPGAMLIGVFGLFGFGALGLTAGARMGGYAWLPLLAWTGLFA
                     TLGWNFLDYGLLNPPAGQGIEWGYVIPGVMFELMAWVPLVFLVATLRGSGDGIGRSSS
                     TAPITANTLRGPGQPGQPSVTVIDMRPPEVQASHRVEVAGPVRKDQLQAIDALFGAAV
                     ADAVADAVAEIPLDPQLRATGSGTAAGDEVEFSEGTQALLDRLERLADMRDRGLLTPE
                     EYETAKDAVMHEIEARS"
     gene            4689..6278
                     /locus_tag="AC162A_01410"
     CDS             4689..6278
                     /locus_tag="AC162A_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009520.1"
                     /translation="MSGTSRRRRALGIGIALLLVVCAFGIPGAATAYTDSDKDGLPNK
                     FERQKSHTNPHRKDTDRDGTKDGNEDPDRDGLSNRYEFLAGTNPRKKDTDKDGKKDGA
                     EDPDKDGLTNVREQAAHTHPRKADTDGDGTRDDREDPDHDGLWNIIEFRADTKPRDAD
                     SDNDGRRDGNEGTRDDALSNGVEQGVGTNPGLKDTDGDGKPDGREDPDADGLMTIEEV
                     VRGLDPKDADSDNDGTLDGDETVAVVDAPVIQGAPDCTVFPADNVWNAKVDALDVASN
                     SSTMISTIGSGRSFHMDFGSYAGYGIPYQVVDGSATKRNVVFDYDDESDAGPYPIPAF
                     PLIEDGSDAHMLLVDRDDCKLYELFAARQSSSGTWHAGSGAIWDMDTNALRPDGWTSA
                     DAAGLPILPGLVRWDEVASGEITHALRFTAPQTRNQYIYPARHEAGSAGASLPPMGMR
                     VRLKDNASVNNMIAGLSDDARVIAIALQRYGMILADNGSAWYISGASDPRFDDDVMHE
                     LDVFEGSNLEVVDTSGLVNGP"
     gene            complement(6499..8211)
                     /locus_tag="AC162A_01415"
     CDS             complement(6499..8211)
                     /locus_tag="AC162A_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR5009521.1"
                     /translation="MEAARTSTSSLAVVERWIPGVRVFRTYERAWLSRDLIAGVVLVT
                     LLVPQGMAYAELAGLPAITGLYTTVVCLIAYAFVGPSPVLVLGPDSSLGPMIAAAILP
                     LAAGDEQVAIGLAGMLALLVGLVTVGAGIARLGFVADLLSNPVRTGYLAGLAVVIFVG
                     QLPKLFGFSTDASGLIAEVGAFLEGLDQTNVWALAIGLLSLGIIVVLRRIAPRTPGIL
                     VAVVVAIGLSMVLDLAARGVSVIGVLPQGFPTPALPSASPSDIPLLLATAVGISLVAI
                     GDTISTSGGFARRVGYEVDGNQELAGIGVANLAAGVFSGFPVSTSGSRTAVAAQSGAK
                     TQLTGLVAAALVLLMLVAAPGLVQAMPQPVLAAVVIAASISLFDVAELRRLYSVRRTE
                     FLLALACALGVALVGVLQGIVIAVVLSAVYVFRRAWAPYSAVLGRSPGVIGYHDVLHY
                     PDAEQIPGLLIVRWSAPLFFANANQFRDRIRGLLEPLDPPPAWVLVVAEPITDIDTTA
                     GSMLADLDAELNASGIHLAFAEMQTAVRDSIVRYGLLETIDEGHFYRSVDEAVDAFHR
                     EVGA"
     gene            complement(8266..9147)
                     /locus_tag="AC162A_01420"
     CDS             complement(8266..9147)
                     /locus_tag="AC162A_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5009522.1"
                     /translation="MTEPITAPPIADPTSGLPIVPDAPVYNASLIRRFDEADSLAYFW
                     VRFDGDPTPFEPGQYMTIGVTVGEKLVQRPYSVASPPAVAGDEGYEFYVRRVQGGTFT
                     PLLFELPVGHRMRMIGPKGKFTLLPDDDRIHIFISSGTGNAPFVAMMRQLLIDGAPRR
                     AVFLNGVSYADELGYRDRVEGWERSGEYPVTFVPTVSRPNDPRNAGWMGRTGRVESIL
                     GPVLEEMGLTPANSIAYICGNPDMILSAEETLLGRGYPEEQVHKELYWPKGKEPRGVA
                     AMDLAAAIEAAEHNADE"
     gene            9372..>9981
                     /locus_tag="AC162A_01425"
     CDS             9372..>9981
                     /locus_tag="AC162A_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR5009523.1"
                     /translation="MATTAAPGTDRAHQAAKRYIYAWGEGTAEGNGRMKDLLGGKGAG
                     LAEMTIAGLPTPPGFTITTEACNDYFAAGKQLPAGLWDDVLEAMHEVERRSGKGFGDV
                     ANPLLVSVRSGAKFSMPGMMDTVLNLGLNEQTLHGLIALTGNERFGWDAYRRFIQMFG
                     RIVMDVNGGRFDGPLEARKELHGHGAADTDLTVDDLKALVEEF"
BASE COUNT         1601 a         3458 c         3512 g         1410 t
ORIGIN      
        1 cgccagccac ggcgacgcct cgacggccgc gtggatcccg cgatccagga tccaggcggc
       61 cagcccgaag gcgccccaga tggccaggta tccggcgacc agcagcgcga cgaggagccg
      121 cggctgcggc cggcggccga cgatcgcgcc gaacgtgacc acgagcggca cgctcgaggg
      181 cagcatcatc gccacgatca tcaggagcca gccgagcgag aacagcgcgg cctccacggg
      241 cagcggcgcg atgccgccgt cgtgatggag gtagcgcccg tacggcgacg ccgcccacac
      301 ccacagcgtc agccaggcga ggccggacag cgcgacgagc gatccggcga ggatcgcccg
      361 atcgcgccgc gggtcgcgga ggtgtccgac cgcgaacatt cgtccggcct ccgtcaggcg
      421 gcgaagcgga agtggccctg gagggcgtta ttgcccttcc ggttcacgtc cggcaggcca
      481 tggggcttgc cgttccgggt gaagtggtgg gacttcgcgg catacaccgg ggagcccggg
      541 atcgtcgaga acaccgtctc cgacagcacc gtggtcttgc cggtcgcgcc cgtgaagggg
      601 atgaggtccg cgtccgcgac ctcgccgatg cgcaggcggc cctcgccgcc gtcgacggtg
      661 aaggtgatcg ggacgcgctc gaccgagacg acctcgccga tgaggccggc gaggtccgcg
      721 atcgcgccgc ccagctgtcc ggtgaacacc ttgagcagct cgccctgctg ctcgtcgctg
      781 gcgccgtcgt cgacgaatac ggcggccttc caggatgcag gcacgagcac gttgcccggg
      841 atgtgcacgg acagcgcaac cgttcgcccg gtgacgtcga cgccctcgat cgagccgctg
      901 tcgatgtgcc acgccagcgc cgagtcgcag gtcccgccgt cggggtcctc gccgacccag
      961 caggggcaga gcacgttgca ggtgcagacc tcgagcagcc tgccctcgat gtcgtatgcc
     1021 atggtcgggt cctccgcact ggttccgact cgcatccgcg ggtccgcatt cacgtcagtc
     1081 ggtcgatcga ccgacatcgg tcgtgtgacc gaaccatacg accgggtgtc aagcccctcg
     1141 ggcgcgccgt ggccgtcgcg cggcgcaata tggatctgtg aacgaactgt ggaccgcgct
     1201 ggtgccgctc gtcatcggca gcgcgatcct gcccgtgcag atcaccgtca cggtcctgct
     1261 gctgcgctcg ggcgccggac ggatcgccgc ggtcgcctgg ctcgccggca tgaccctcgt
     1321 ccggctcgct caggggatca tcttcggctt cgtgttcggt gtcggcgtgg ccgacgccca
     1381 gggacccgcc cggcccgggc ccatcgcttc aaccatgctc ctcgtggtgg gcgtcctgtt
     1441 cctcgtgagc gccgtccgca agctgctcga cgatccggac gatgacgcac cgccgccaac
     1501 atggatggcg atgatgtcca ccgcgacccc gggccgtgcg ttcgggttcg gtgcgggcgt
     1561 cgttggggcg agcccgaagc tgtgggcgtt cacccttggt gccatcgccg tcatcgcgga
     1621 ggcggatctc ggccagccgg ctgcagccgg catgttcctc gtgttcgtcg tgctcgcgga
     1681 gtcgatccac ctggcgctgg tggccttcgc gtacgcggcg ccgcgccgcg cggagcccgc
     1741 gcttgaccgc gtgacggagc tcctggcgcg ttacaaccgg ccgttgatga tcgggctggg
     1801 cttggtgttc ggggtctggt tcctggccaa ggccctcgac ggctttggcg tcctctagct
     1861 cgtcggctcg cccagcgagc catgcagcgg ttctctgccc cttttgctcg cccggcgagc
     1921 gatctggcgg ctgcgggccc cgatagccgc tgggctgcac gctcggggag caagccgccg
     1981 ggagaaccgc tgtcctgctc gtcaggcgag cagcggcggg ggagggaccc aatcggcgtg
     2041 cctgctacca tccggccacc agtcatcccg cgcggccctt ccacggtcgc gtaccagcgg
     2101 agcaccctgt gaccaccccc accgtcgacc ccggcgacgt cgcgcccgcc gttgccgcgc
     2161 tggacacgat cgtctccctg tccaagcgcc gcggcttcat ctttccctcg tccgagatct
     2221 acggcggcat caacgccgtg tgggactacg ggcccctcgg cgtcgagctc aagaacaacg
     2281 tcaagcgcgc gtggtggcgg gcgatggtcc aggaccgcga cgacatcgtg ggcctcgatg
     2341 ccgggatcct gatgcatccc caggtgtggg tcacctccgg ccatgtcggg tcattcagcg
     2401 accccctggt cgagtgcgcg acgtgccacc gccgctatcg cctcgacgag ctcccgggcg
     2461 ccgaggacct gagcgccacg gacctgcggg acgaaaccat cgtcagccgt ctcggcctcg
     2521 tgtgccccaa cgacgccggc ccgctgtcgg cgccccgtcg gttcaacctg atgttcacct
     2581 cgcacatggg cccggtggag gacgacgcct cgatcgtcta cttccgcccc gagaccgcgc
     2641 agggcagcta cgtcaacttc aagaacgtgc agcagagctc gcgcaagaag atcccgttcg
     2701 gcatcgccca aatcggcaag tcgttccgca acgagatctc gcccggcaac ttcgtgttcc
     2761 ggatgcgcga gttcgagcag atggagatgc agtacttcgt ccgcccggac acgggggcct
     2821 cggaggcctt cgagtactgg ctgccgcgac gccgggcctg gtacgaggcc tacggcgtga
     2881 cgccgtcgcg cctccgattc cgggagcacg cgcccgacga gctcgcgcac tacgccaaga
     2941 aggcgatcga cgtcgagtac cgcttcccgt tcggctggaa ggagctcgag ggcatccaca
     3001 accggggcga cttcgacctc ggcaaccacg ccaaggcgtc gggggagaac ctcgagtact
     3061 tcgaccaggc cacggaggag cacttcatcc cctggatcgt ggagacggcc gcgggcgcgg
     3121 accgtgcggc gttcacgttc ctcatcgacg cctaccgcga ggaggaggtc cggggtgaga
     3181 cacgggtcgt cctcgggctg catccggacc tcgcgccgta caaggtcgcc gtgctgccgt
     3241 tgctcaagaa gcggcccgag atcgtcgaga tgtgccaccg cctgctggcc gacctcaagc
     3301 gcgacctgat ggcggtctac gacgacacgg ccgcgatcgg caagctctat cgccgccagg
     3361 acgagatcgg gacgccgtgg tgcgtgacgg tggacgtcga ctcgctcgag gacggcgcgg
     3421 tcacgatccg tgaccgcgac accatgggcc aggagcgcgt accgctcgag ggcgtgaagc
     3481 gcctgctgct cgaccggatg gccgccgtcc ggccctgacc cggtcgctag actggatcgg
     3541 tgacccgagc cacacgcgag gatccgcggc tgcgcgacgt gctgacgctc gcgatcgcgg
     3601 tcgccgggct cgcggcctgt atcacgctca tctacctcgc gatgcgggcc gtcatggatg
     3661 tgggcggggc gtgtgccgac ggcggaccct acgtctcggc ccagccctgt ccggacggca
     3721 cgccgggagc gatgctgatc ggggtgttcg ggctgttcgg gttcggggcg ctggggttga
     3781 cggccggcgc ccgcatgggc ggctacgcct ggctgccgct gctcgcttgg accgggctgt
     3841 tcgcgaccct gggctggaac ttcctcgact acggcctgtt gaatccacca gcagggcagg
     3901 ggatcgagtg ggggtacgtc atccccggcg tgatgttcga gctcatggcc tgggtgccgc
     3961 tcgtgttcct ggtcgcgaca ctgcgcgggt caggagatgg gatcggtcgg tcttcttcga
     4021 cggccccgat caccgccaac acgctccgtg ggcctgggca gccggggcag ccatcggtca
     4081 ccgtcatcga catgcggcca ccggaggtgc aggccagcca tcgtgtcgag gtcgcgggcc
     4141 ctgtccggaa ggaccagctc caggccattg acgccctgtt cggcgccgcg gtggccgacg
     4201 ccgtcgccga tgcggtggcg gagatcccgc tcgacccgca actgcgcgcg accgggagcg
     4261 gcacggctgc cggcgatgag gtggagttct ccgaggggac gcaggcgttg ctcgaccggc
     4321 tcgagcgcct ggcggacatg cgcgatcgcg gcctgctgac gccggaggag tacgagacgg
     4381 ccaaggatgc cgtgatgcac gagatcgagg ctcgctcgtg acgatcacgc gccggatcgt
     4441 gccactgctg ctggtcgcgg ccgggatcgt cggcgtcgcg gtcggcgccc gggtgttcgc
     4501 gttctttgcc ggcggttagg gtcctggctc gcgaactgcg gtgcccgtcg gcaccgaccg
     4561 ggacctccgg gtcatcgtcg cccagaccga ccgcgcgcga ggatcggggg catgggcgcg
     4621 gtcgccaggc cggtctgcaa cacgaccgag ctcgacggtc gcgcccaccc agatacacgg
     4681 gaggcgggat gagcgggacc agcaggcgac ggagggcgct cggcatcggc atcgccctgt
     4741 tgctggtggt ctgcgccttc gggatccccg gggcggcgac cgcgtacacg gactcggaca
     4801 aggacgggct cccgaacaag ttcgagcggc agaagtcgca cacgaacccg catcgcaagg
     4861 acaccgaccg cgacggcacg aaggacggga acgaggatcc cgaccgggac gggctctcga
     4921 accgctacga gttcctcgcc gggacgaacc cgcgcaagaa ggacaccgac aaggacggca
     4981 agaaggacgg cgccgaggac cccgacaagg acggcctgac caacgtccgg gagcaggccg
     5041 cgcacaccca tccgcgcaag gccgacaccg acggcgacgg cacccgcgac gaccgcgagg
     5101 atcccgacca tgacggcctg tggaacatca tcgagttccg ggccgacacc aagccacgcg
     5161 acgccgactc cgacaacgac gggcgccgcg acggcaacga gggcacccgc gacgacgcgc
     5221 tctcgaacgg cgtcgagcag ggggtcggga cgaacccggg gctcaaggac accgacggcg
     5281 acggcaagcc ggacggccgc gaggatcccg acgccgacgg cttgatgacg atcgaggagg
     5341 tggtccgcgg cctcgatccc aaggacgccg actccgacaa tgacgggacg ctcgacggcg
     5401 acgagaccgt ggccgtcgtc gacgcgccgg tcatccaggg cgcgcccgac tgcacggtgt
     5461 tcccggccga caacgtctgg aacgcgaagg tcgacgcgct cgacgtcgcc tcgaacagct
     5521 cgacgatgat ctcgaccatc ggcagcggcc gctcgttcca catggacttc ggctcgtacg
     5581 ccggctacgg gatcccgtac caggtcgtcg acggttcggc gacgaagcgg aacgtcgtgt
     5641 tcgactacga cgacgagtcc gacgctggcc cgtatccgat cccggcgttc ccgctgatcg
     5701 aggacggctc cgacgcccac atgctcctgg tcgatcgcga cgactgcaag ctgtacgagc
     5761 tgtttgcggc ccgccagtcc tcgagcggga cgtggcatgc gggttcaggc gcgatctggg
     5821 acatggacac gaacgccctg cgtccggacg gctggaccag cgccgacgcg gcggggctgc
     5881 cgatcctgcc cgggctcgtc cgctgggatg aggtcgcgtc gggcgagatc acccacgcgc
     5941 tgcggttcac cgcgccacag acccgcaacc agtacatcta tccggcgcgc cacgaggcgg
     6001 gctccgcggg cgcgtccctg ccgccgatgg gcatgcgggt gcgcctgaag gacaacgcga
     6061 gcgtgaacaa catgatcgcc ggcctctccg atgacgcccg cgtcatcgcg atcgcgctcc
     6121 agcgctacgg gatgatcctg gccgacaacg gctcggcctg gtacatctcc ggcgcgagcg
     6181 acccgcgctt cgacgacgac gtgatgcacg agctggacgt gttcgagggc tcgaacctgg
     6241 aagtggtcga taccagcggt ctcgtcaacg gcccgtagtc ggccagccct gtccggccgg
     6301 tgcgcgccga tgcgtggcgc gcatggattc cggtcgcggc gaccaacttg atgcgtggca
     6361 cgcacggatt cgagccgatt tccgcctgat catgcccgat tcgtgccgtc gcgatgcgtg
     6421 cggcgcacgt tccgcgcact tggagcaggg attgatgcgt gccacgcacg gtggcgactc
     6481 aggtcgtcgg ccggatgctc aggctccgac ctctcggtga aatgcatcga ccgcctcatc
     6541 gaccgaccgg tagaagtgac cctcgtcgat ggtctcgagc agcccgtagc gcacgatcga
     6601 gtcacggacg gcggtctgca tctcggcgaa cgcaaggtgg atgccgctgg cgttcagctc
     6661 ggcatcgaga tcggccagca tggacccggc cgtggtgtcg atgtcggtga tcggttcggc
     6721 caccacgagg acccaggcgg gcggtgggtc gagtggctcg agaagaccgc ggatccggtc
     6781 gcggaactgg ttggcattgg cgaagaacag cggagccgac cagcgaacga tgagcagccc
     6841 agggatctgt tcggcgtccg gatagtgcag gacgtcgtga tacccgatca cgccgggact
     6901 ccttccgagg accgccgagt atggcgccca ggcacgcctg aagacgtaga ccgccgagag
     6961 gacgaccgcg atgacgatcc cctggaggac gccgacgagc gccaccccga gcgcgcaggc
     7021 cagggcaagg aggaattcgg tcctgcgcac ggagtagagg cgccgtagct cggcgacgtc
     7081 gaacaggctg atcgacgccg cgatgacgac ggccgcgagg accggctggg gcatggcctg
     7141 gacgagcccg ggcgcggcga ccagcatcag gagcacgagc gcggcggcga cgaggccggt
     7201 cagctgcgtc ttcgccccgg actgggccgc gaccgcggtg cgcgaaccgc tggtgctcac
     7261 ggggaacccg gagaacacgc cggccgccag gttggcgacc ccgatcccgg ccagctcctg
     7321 attcccatcg acctcgtagc cgactcgtcg ggcgaacccg cccgacgtcg agatcgtgtc
     7381 gccgatcgcg accagcgaga tcccgacggc ggtcgccagc aggagcggga tgtccgaggg
     7441 cgatgctgac ggcagcgcgg gcgtcgggaa cccctgcggc aggacgccga tgaccgacac
     7501 gccacgcgcg gcgaggtcga ggaccatcga gagcccgatc gcgaccacga cggccacgag
     7561 gatgcccggc gtgcgcggtg cgattcgccg gaggaccacg atgatgccga ggctgagcag
     7621 gccgatggcg agggcccaga cgttggtctg gtcgagaccc tcgaggaagg caccgacctc
     7681 cgcgatcagc ccgctcgcat cggtcgaaaa accgaacagc ttcggcagct gaccgacgaa
     7741 gatcacgacg gcgaggcccg cgagataccc cgttcggacc gggttcgaca ggaggtcggc
     7801 gacgaacccg agccgggcaa tgccggcgcc gaccgtgacg aggcccacca acagggcaag
     7861 catcccggct aggccgatcg cgacctgctc gtcaccggcg gcaagtggca ggatcgcggc
     7921 cgcgatcatc ggtccgagcg acgagtcggg accgaggacg aggacgggcg acggtccgac
     7981 gaaggcgtag gcgatgaggc agacgacggt ggtgtacagc ccggtgatgg cgggcaatcc
     8041 ggccagctcc gcgtaggcca tcccctgtgg gacgagcagc gtgacgagca cgacgcccgc
     8101 gatcaggtcg cgagacagcc acgcacgttc gtaggtccgg aagacccgga cgcccggaat
     8161 ccaacgctcc acgacggcga gcgaggaggt cgaagttcga gcggcttcca ccgttcctcc
     8221 ttcgagcgcg gcgcttggga ctaggaggtc gaggtgggcc tgatcctact cgtcggcgtt
     8281 gtgttcggcg gcctcgatcg cggcggcgag gtccatggcg gccaccccgc ggggctcctt
     8341 gcccttgggc cagtagagct ccttgtggac ctgctcctcg gggtagccgc ggccgagcag
     8401 cgtctcctcg gccgacagga tcatgtccgg gttgccgcag atataggcga tcgagttggc
     8461 gggcgtgagg cccatctcct ccaggaccgg ccccaggatc gactccaccc ggccggtccg
     8521 tcccatccag cccgcgttgc gcgggtcgtt gggccgcgac acggtcggga cgaacgtcac
     8581 cgggtactcc ccggatcgct cccagccctc gacccggtcg cgatagccca gctcgtccgc
     8641 gtacgagacg ccgttgagga acaccgcccg ccgcggcgcc ccgtcgatca ggagctggcg
     8701 catcatcgcc acgaacggcg cattgcccgt gcccgacgag atgaagatgt ggatccggtc
     8761 gtcgtcgggc agcagcgtga acttgccctt gggtccgatc atccgcatcc ggtggcccac
     8821 gggcagctcg aacagcagcg gcgtgaacgt cccgccctgc acccggcgca cgtagaactc
     8881 gtagccctcg tccccggcca cggccggcgg cgacgcgacg gagtacggcc gctgcacgag
     8941 cttctcgccg accgtgaccc cgatcgtcat gtactgcccg ggctcgaagg gcgtcggatc
     9001 gccgtcgaag cggacccaga agtaggccag cgagtcggcc tcgtcgaagc gcctgatcag
     9061 gctcgcgttg tagacgggag cgtccggcac gatcggcagt cccgaggtcg ggtcggcgat
     9121 cgggggcgcg gtgatcggtt cggtcatcgc gggcagtggt ctccgggcac ggcggaacgt
     9181 cgtgtggatc gtaccccgcg aagacgcacg cggacggggg ccggacgggt ggttcagggg
     9241 tgccgatcgg ccccacccga caccgatccg caacccgggt cgaccagagg acacgcgtac
     9301 aatcgcaacc tgcagcgaag ccttccccgc gcccgcccac gcgcggcgcc gacgccgagg
     9361 agcccgtccc gatggccacg acagccgcac ccggcaccga ccgcgcccac caggccgcca
     9421 agcgctacat ctacgcctgg ggcgagggga ccgccgaagg caacggcagg atgaaggacc
     9481 tgctcggtgg caagggtgcg ggcctggccg aaatgaccat cgccggcctg ccgaccccgc
     9541 cgggcttcac gatcaccacc gaggcctgca acgactactt cgccgcgggc aagcagctcc
     9601 cggcaggcct ctgggacgac gtcctggagg cgatgcacga ggtcgagcgc cgctcgggca
     9661 agggcttcgg cgacgtcgcc aacccgctgc tcgtgtccgt ccgctcgggc gccaagttct
     9721 cgatgcccgg gatgatggac acggtcctca acctgggtct caacgagcag acgctgcacg
     9781 gcctgatcgc gctcaccggc aacgagcgct tcggctggga cgcgtatcgc cggttcatcc
     9841 agatgttcgg ccggatcgtc atggacgtga acggcggccg gttcgacggc ccgctcgagg
     9901 cccgcaagga gctccacggc cacggcgccg ccgacacgga cctgacggtc gacgacctca
     9961 aggcgctcgt cgaggagttc a
//
LOCUS       DBNHXO010000063         4496 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3389997, whole genome shotgun sequence.
ACCESSION   DBNHXO010000063 DBNHXO010000000
VERSION     DBNHXO010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3389997"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="AC162A_01430"
     CDS             complement(<1..319)
                     /locus_tag="AC162A_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013219.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HIR5009524.1"
                     /translation="MTTTAAPAGAPGRLAIGVSVRRVTRTGWLVGAGLVVLVLVFFAG
                     VLLGSVDLSPATTIGIIANRLGFNVPVTWPRTSEAIVMDLRLPRVLTAMVVGTGLAVA
                     GATF"
     gene            complement(339..1391)
                     /locus_tag="AC162A_01435"
     CDS             complement(339..1391)
                     /locus_tag="AC162A_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5009525.1"
                     /translation="MDARSRATHRRRHHAFPALLLLLLATVLAACTTPAASPSATEAA
                     LATATPAPSVATPEPTAAPTPTPAPTFPLTLTDDEGNEVTLPAAPTKIASITNAATEI
                     LFALGVGDQIVGKVEEFTPYPPEVADIPDVAKFGSVDVEKIVGLGTELVIAGGSNFNP
                     PDAIAKLRQLGVPVLVVFAPDVETALADITLIGSAVGRDEEAAKLTSSIQASFDQVAA
                     ATEGLPKPRVYYELDASNGFFGPAPDYFGTEMIEIAGGDPLTSGTDGVYQLEEEKILA
                     FDPEVILLGDAAYGVTPEQVAARPGWDTLTAVKNGDVRPIDDVIVTRPGPRLGEGIRA
                     LATAIHPDIVLPSAAP"
     regulatory      complement(1476..1619)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01482"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The adenosylcobalamin (AdoCbl) riboswitch is found
                     in the 5'-untranslated regions of vitamin B12 (Cobalamin)
                     related genes in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF01482"
     gene            1752..2831
                     /locus_tag="AC162A_01440"
     CDS             1752..2831
                     /locus_tag="AC162A_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009526.1"
                     /translation="MEVIRFARALVPLAAITLMSTPGAMRPAVSGTADPPWDPPACLV
                     AVGVPPAAAGTAWYRTDATLDASGTLAGQQLTVGVVGGGERRVALPAESFASGPVRGL
                     VTVGDDDGTRSRLRVVDPAHGCAWTIAVEATVIRSAILAPDAGSTWEHRVDRATRADL
                     GVWRRPVSGGIASRVAPGLAPDVAHGRTFATDLRWAPDGRRVIASCGELACRTRLVDP
                     ATGLTEMTSRTGPVIGVAGTRVIAYGVCPGLPCPVRATEPSTGAVETLVDEAGPAALA
                     VGALVYERDGHLVSQDLRTRVVSDVAASDGLAPLPGGSGATAGMDLPAGSVTLAAAGQ
                     RPAPSTARRFDPFTSAIGPIEAVQR"
     gene            2828..3856
                     /locus_tag="AC162A_01445"
     CDS             2828..3856
                     /locus_tag="AC162A_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HIR5009527.1"
                     /translation="MITRLHAFAGVALALIIVGALAAPAAAHGPDPILGGGLYAQNLD
                     LKYRWAGGGTPPAAMKSAINDAADDSNATRKSKAPTFDYAASADNVVYYGSDVPCGVN
                     GLACMRRDPPDWFGVWFRENGHRFDWGTLRWCELAGGPDGCYQVENVMLDELGHVLVL
                     DHHDNFGDDSDYGDAVVQTYSRTKPKAGWNAHAYGRCDVATLQQQYDVANSSTLYSTC
                     LDVPTELSVSPSRSSVVAGGMVTFTATLGTDGTGKLSNNLVSGRTVVLQQRLSSGWSD
                     ILTMNAASEAGTYTGSVSVWATRDYRALFRKPANEGLKSSSSPARTVTVTAPCRQPCP
                     QLEGGSGG"
     gene            3853..4449
                     /locus_tag="AC162A_01450"
     CDS             3853..4449
                     /locus_tag="AC162A_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009528.1"
                     /translation="MTSPTVVSSGGGAHRWLALGALVVVVAACSGPAGSPSAASGTPA
                     PASAEVPATPAAPASEAPSASAEATADPGGSSEPPAALLIGTDAGPVAGDLGTLSWDG
                     LVSDSPWIVQRSGNAAAAGARLRVRFDDSMDQTRWTARWAPIRRGEAGTPRAAGSGDE
                     GRILIEAPAEAGSWSLQLEARFGDGRRAAWYWRVAVDG"
BASE COUNT          601 a         1576 c         1684 g          635 t
ORIGIN      
        1 ggaacgtcgc ccccgcaacc gccagccccg tcccgaccac catcgccgtc agcacccgcg
       61 gcagccgcag gtccatgacg atcgcctccg acgtccgtgg ccaggtgacc ggcacgttga
      121 agccgagccg gttggcgatg atcccgatcg tggtcgccgg cgacaggtcg acggagccga
      181 gcaggacgcc ggcgaagaac acgagcacga gcacgacgag gcccgcgccg accagccacc
      241 ccgtccgggt cacccggcgg acggacacgc cgatggccag gcgcccgggg gcgcctgccg
      301 gggcggccgt ggtggtcatc gcgggcctgc cggcatcgtc agggcgccgc cgagggcagc
      361 acgatgtccg ggtggatcgc ggtcgcgagt gcacggatgc cctcgccgag gcgcggtcct
      421 ggccgggtca cgatgacgtc gtcgatcggg cgaacgtcgc cgttcttgac ggcggtcagg
      481 gtgtcccagc ccggtcgtgc ggcgacctgc tcgggcgtga ccccgtaggc ggcgtcgccc
      541 agcaggatca cctccgggtc gaaggcgagg atcttctcct cctcgagctg gtagacgccg
      601 tccgtgccgc tggtcagcgg atcaccgccg gcgatctcga tcatctcggt gccgaagtag
      661 tccggggccg gcccgaagaa gccgttcgat gcgtcgagct cgtagtacac gcgcggcttc
      721 gggagacctt cggtcgcggc ggcgacctgg tcgaacgacg cctggatcga ggacgtcagc
      781 ttggccgcct cctcgtcgcg gccgaccgcg ctgccgatca gcgtgatgtc cgcgagggcc
      841 gtctcgacgt ccggcgcgaa gacgacgagg acgggtacgc cgagctggcg cagcttcgcg
      901 atggcgtcgg gcgggttgaa gttgctgccg ccggcgatca cgagctccgt gccgaggccg
      961 acgatcttct ccacgtccac cgacccgaac ttggcgacgt cggggatgtc ggcgacctcc
     1021 ggcggatagg gcgtgaactc ctcgaccttg cccacgatct ggtcgccgac gccgagggcg
     1081 aacaggatct ccgtggccgc gttcgtgatc gacgcgatct tggtcggggc ggcggggagg
     1141 gtgacctcgt tgccctcgtc gtcggtgagg gtgagcggga aggtgggtgc cggcgtcggc
     1201 gtgggcgctg cggtcggctc gggcgtggcg accgacgggg cgggcgtcgc cgtcgcgagc
     1261 gcggcctcgg tggcgctcgg gctcgcggcc ggcgtcgtgc aggcggcgag gaccgtcgca
     1321 aggagcagga gcaacagggc ggggaaggcg tgatggcgac ggcgatgcgt cgcgcgggag
     1381 cgtgcgtcca cgggtactcc gggtggcagg ggttggagga aacgaaagac ccctctcttc
     1441 gggatcgaag gaggggtcag gcggtagatc gcacgatgcg cctgaccttt ctctcgaagg
     1501 ctcgagcggc gacggcggat cggcgaccgg acttccgccc caatggggcg gtcaccgtag
     1561 cgggactgtg ccggactcac accggcttcg cgatcacacc gcgtcggggc tattcagggc
     1621 ctcacgggcc cggctgatgg ccgcagcgta ccaccggccc cgggcgataa gtccgggata
     1681 agcggccatc gcgacactgg tgacctcgcc ctcctcccgc ccggtcgtgt ccccgccgga
     1741 ggaggtcact cgtggaggtg atccgattcg cgcgtgcgct cgtgcccctg gcggcgatca
     1801 cgctgatgag caccccggga gccatgcggc ccgcggtgtc cgggacggcg gacccgccct
     1861 gggatccgcc cgcgtgcctc gtcgccgtgg gcgtgccgcc ggccgcggcg ggcacggcgt
     1921 ggtaccgcac ggacgccacg ctcgatgcgt cgggcacgct cgcgggccag cagttgacgg
     1981 tcggcgtcgt cggcggaggc gagcggcggg tcgcgctgcc cgcggagtcg ttcgcgtccg
     2041 ggccggtgcg cggcctcgtg acggtcggcg atgacgacgg gacccgctcg cggctgcgcg
     2101 tcgtcgaccc ggcacatggc tgcgcctgga cgatcgccgt cgaggcgacc gtcatcagga
     2161 gtgcgatcct ggcaccggat gccggctcga cctgggagca ccgtgtcgac cgggcgacgc
     2221 gtgcggacct cggcgtctgg cggcgtcccg tcagcggcgg catcgcgtcg cgggtcgcgc
     2281 ccgggctcgc ccccgacgtt gcccacggcc ggacgttcgc gacggacctg cgctgggcgc
     2341 cggacgggcg gcgtgtgatc gcgtcctgtg gtgagctcgc ctgccggacc cggctcgtcg
     2401 atccggcgac cggcctgacc gagatgacga gtcgcaccgg gcccgtgatc ggtgtcgccg
     2461 gcacacgcgt gattgcgtac ggcgtttgcc cgggacttcc gtgcccggtg cgggcaaccg
     2521 agccatccac gggcgccgtc gagaccctcg tcgacgaggc tgggccggca gccctggcgg
     2581 tcggcgcgct ggtgtacgag cgcgatggcc atctcgtcag ccaggacctg cggacacgag
     2641 tcgtctcgga cgtggcggca tcggatggcc tcgcccccct gcctggcggc tcgggcgcca
     2701 cagccggcat ggacctgccc gccggttcgg tgacgctcgc ggcggccggg caacggcctg
     2761 ccccatcgac cgctaggcga tttgacccgt tcacctccgc catcggaccc atcgaggcgg
     2821 tgcagcgatg atcaccaggc tccacgcatt cgccggagtg gctctggcgc tgatcatcgt
     2881 cggcgcgctc gcggcgcccg ccgccgcgca cggcccggac ccgatcctcg gcggcgggct
     2941 gtatgcccag aacctcgacc tcaagtaccg ctgggccggc ggcggcactc cgcccgccgc
     3001 gatgaagagc gcgatcaacg acgccgctga cgattcgaac gccacgcgca agtcgaaggc
     3061 gccgaccttc gactacgccg cctccgcaga caacgtcgtg tactacgggt ccgacgtccc
     3121 gtgtggcgtc aacggcctcg cctgcatgcg ccgcgaccca cccgactggt tcggcgtgtg
     3181 gttccgcgag aacggtcacc ggttcgactg gggcacgctc cgctggtgcg agctcgccgg
     3241 cgggccggac ggctgctacc aggtcgagaa cgtgatgctc gacgagctcg ggcatgtcct
     3301 cgtcctcgac caccacgaca acttcggcga cgacagtgac tacggcgatg ccgtggtcca
     3361 gacctactcc cggacgaagc ccaaggccgg ctggaacgcc cacgcctacg ggcgttgcga
     3421 cgtggcgacc ctccagcagc agtacgacgt cgcgaactcc agcacgctct acagcacctg
     3481 cctcgacgtt cccaccgagc tgtcggtctc accatcgagg tcctcggtcg tcgccggtgg
     3541 catggtgacg ttcacggcga cgctcgggac ggacggcacc gggaagctct cgaacaacct
     3601 ggtgagcggc cgcacggtcg tgctccagca gcgtctgagc tccggctgga gcgacatcct
     3661 gacgatgaat gcggcgtccg aggccggcac ctacaccggc agcgtgagcg tctgggcaac
     3721 gcgcgactac cgggcgctgt tccgcaagcc ggcgaacgag ggcctcaagt cgtcgtcctc
     3781 gccggccagg accgtgaccg tgaccgcgcc ctgccggcag ccctgtccac agctcgaagg
     3841 agggtcaggc gggtgacgag tccgacggtt gtctcgagtg gaggcggagc gcatcggtgg
     3901 ctggcgctcg gagccctcgt cgtggttgtg gccgcctgtt cgggaccggc gggctccccg
     3961 tcggcggcga gcggcacgcc ggctcccgca tcggccgaag tcccggcgac gccggccgcg
     4021 cctgcgagtg aggctccgtc cgcatcggcc gaggcgacgg ccgaccccgg cggctcgtcg
     4081 gagccgcccg cggcgctgct gatcgggacc gatgccgggc ctgtggcagg cgatctcggc
     4141 acgttgtcgt gggacggtct cgtgtccgac tcgccgtgga tcgtccagcg ctcaggcaat
     4201 gccgccgcag ccggcgcacg cctgcgggtc aggttcgacg attccatgga ccagaccagg
     4261 tggacagcgc gctgggcgcc gatccgccgc ggcgaggctg gcacgccacg ggcggccggc
     4321 tcgggcgacg agggccggat cctgatagag gcgccggccg aggccggctc gtggagcctg
     4381 cagctggagg cgaggttcgg cgacggccgc cgggcggcct ggtactggcg ggtcgccgtc
     4441 gatggctgat gcggtgcgct gggccggcct cgtggccggc cggctcagac cagggc
//
LOCUS       DBNHXO010000064         3766 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2794835, whole genome shotgun sequence.
ACCESSION   DBNHXO010000064 DBNHXO010000000
VERSION     DBNHXO010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2794835"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="AC162A_01455"
     CDS             complement(<1..209)
                     /locus_tag="AC162A_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5009529.1"
                     /translation="MRITSLALTDLRAYHDREFELSPGLNIVRGPNEAGKSTIQRAIE
                     LALTRKVTSSAGDLDGLVPWGSSEDA"
     gene            complement(206..1384)
                     /locus_tag="AC162A_01460"
     CDS             complement(206..1384)
                     /locus_tag="AC162A_01460"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR5009530.1"
                     /translation="MLRLLHTADVHLGARHADLGEAAAAQRERQFAAFRAAVDLAIEE
                     KVDLFLVAGDLFDSNVQPKRSVERVAAELGRLATARIRSVLVPGTHDVYDRSSVYRAY
                     DLAALAGTRPAEEMVTVLTPDHPWIHLASVDAVIHGPCFPTKRAPMSPLRDLAAVETP
                     EAAWRIGLLHASVAIPGKTDNDEVVVTVEEIAASGLDYLALGHWHSAQVAKTKGVTYA
                     YAGAPEPVAVDQDKAGKVLLVTLDVDGAGQHTVEVDQRVVGRTTFERREIDAATIDSQ
                     PALITQLTQGADPDLVLDVRVIGVRPDGLDLEPGEVEQALRGSFLRVRVRDVSHPALT
                     EGLLPPAETIAGAFIRNVEGRIADLEASGDDPARREADELRDVLRLGRLLLAGSEVTL
                     "
     gene            1830..2171
                     /locus_tag="AC162A_01465"
     CDS             1830..2171
                     /locus_tag="AC162A_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HIR5009531.1"
                     /translation="MTYVDRSLNCVDCGVEFIHSAADQEYYVQKGFTSDPKRCASCRA
                     YRRTTRDGGSADVRDIGGPRGYERTDDRMSREYFVAICTSCGNQAQVPFKPRMDKPVY
                     CSDCFRSIRPD"
     gene            complement(2259..2981)
                     /locus_tag="AC162A_01470"
     CDS             complement(2259..2981)
                     /locus_tag="AC162A_01470"
                     /inference="COORDINATES: protein motif:HMM:NF015534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HIR5009532.1"
                     /translation="MPGPVALVGAGEFLAPMAEFDRGLLEATGRPRPRVVVLPTAAAP
                     DGEQIFRDWAEMGIEHFSALGAEVEPVLVRNAAEGFDGAALQAIGEADLIYLSGGHPK
                     HLLDVLQQSPLGGAIRAANDRGAVIAGCSAGAMAIVGRTVDFRFLPKVRMPMPFPVRW
                     PIGLALVDGVAVLPHYDAWPEPFSAFVALQAPRGGVILGIDEDTAAVGCNNAWLVHGR
                     GRVTIWRGRHRERFRRGEAFRI"
     gene            3054..>3766
                     /locus_tag="AC162A_01475"
     CDS             3054..>3766
                     /locus_tag="AC162A_01475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018503290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5009533.1"
                     /translation="MTATSGRGGPRDWDATTYDQVAAPMTRWGSDVLERLPLRGDETV
                     LDAGCGTGQITEQLVERLPRGRVVALDGSPAMVEAARGRLARFGERVDFVIADLAAPL
                     PLADASVDAILSTATFHWVADHGALFRHLGRVVAPGGRLVAQCGGEGNIVQLRAALDE
                     VGDGWEGPWNFASPEETRRRLEAAGFVGIDTWLTDEPTSFEPGPAFRDYLRTVALSAH
                     VARLEPEAQDDFLDAVIERL"
BASE COUNT          522 a         1371 c         1367 g          506 t
ORIGIN      
        1 gcgtcctcag agctgcccca cgggaccagc ccatcgaggt ccccggcgga gctggtgacc
       61 ttgcgggtca gcgcgagctc gatcgcccgc tggatcgtcg acttgccggc ctcgttgggc
      121 ccgcggacga tgttcagacc gggcgacagc tcgaactcgc ggtcgtggta ggcgcgcagg
      181 tcggtcaggg cgaggctcgt gatcctcaca gcgtgacctc ggaacctgcc aggagcagcc
      241 gccccagccg gaggacgtcg cgcagctcgt cggcctcgcg gcgagccggg tcatcgccgg
      301 acgcttcgag gtctgcgatt cggccctcga cgttgcggat gaacgcgccc gcgatcgtct
      361 ccgcgggcgg cagcaggccc tcggtgagcg ccggatggct cacgtcgcga acgcgcacgc
      421 gcaggaacga cccacgcagg gcctgctcca cctcgcccgg ctcgaggtca agcccgtcgg
      481 gccgcacgcc gatcacccgc acgtcgagca cgaggtccgg gtcggcgccc tgggtcagct
      541 gggtgatcag cgccggctgg gagtcgatcg tggccgcgtc gatctcgcgg cgctcgaagg
      601 tggtccggcc gacgacccgc tggtcgacct cgacggtgtg ctggccggcc ccatcgacgt
      661 cgagcgtcac caggaggacc ttgccggcct tgtcctggtc gacggcgacc ggctcgggcg
      721 ccccggcgta ggcgtaggtg acgcccttcg tcttcgcgac ctgggccgag tgccagtggc
      781 cgagggcgag gtagtcgaga ccgctggcgg cgatctcctc caccgtgacc acgacctcgt
      841 cgttgtcggt cttgccgggg atcgcgacag aggcgtgcag gaggccgatg cgccaggcgg
      901 cctcgggggt ctcgacggcg gcgaggtccc ggagcgggct catcggcgcg cgcttggtcg
      961 ggaagcacgg gccgtgaatg acggcgtcga ccgatgccag gtggatccag gggtggtccg
     1021 gcgtgaggac ggtgaccatc tcctcggccg gccgggtgcc ggccaatgcc gccaggtcgt
     1081 aggcccggta gaccgacgag cggtcgtaga cgtcgtgtgt gccggggacc agcaccgacc
     1141 ggatgcgggc cgtggcgagc cggccgagct cggcggcgac ccgctccacg gaacgcttcg
     1201 gctgcacgtt cgagtcgaac aggtcgccgg ccacgaggaa caggtcgacc ttctcctcga
     1261 tggccaggtc cacggcggcc ctgaacgccg cgaactggcg ttcccgctgt gccgcggcgg
     1321 cctccccgag gtccgcgtgg cgcgcgccga ggtggacgtc tgcggtgtgc agcagtcgaa
     1381 gcatcgggtg cggctgccct ccctggtctc cgtcccgttc gttcgcaccc ggcgccggca
     1441 agccgaaccg ggacccgcga gcagccgctc ccgccctgcg cacccgtgac ctaggctcga
     1501 tcgcgggccg ccttgggccg ggccggtcgc ccccgggacc cggtttgcga tccgggcgcc
     1561 cccattgctc ccggctcgcg acccgagtct tgacctccat tgtgacaggt cggctgtcac
     1621 gcccggaggg gtgccgagag cacggttcga caacccggtg taggctcagg gccactcaac
     1681 taccggtgcg ccccgagcta gggactccga gaacggcacc tggatcgcgc ggcctcggct
     1741 gactccccgc cgcaagcgcc gtccaggcgt cggaaccctt gcaggcgcgc accagaccat
     1801 gtccgagcgc gcgcccggga ggagcagcaa tgacctacgt cgaccgaagc ctgaactgcg
     1861 ttgactgcgg ggtggagttc atccactcgg cggcggacca ggagtactac gtgcagaagg
     1921 ggttcacctc ggaccccaag cgctgcgcct cgtgccgcgc ctaccggcgc acgacgcgcg
     1981 acggcgggag cgcggacgtc cgcgacatcg gcggcccgcg cggctacgag cgcaccgacg
     2041 accggatgtc ccgcgagtac ttcgtggcca tctgcacgtc gtgcggcaac caggcgcagg
     2101 ttccgttcaa gccgcgcatg gacaagcccg tctactgctc cgactgcttc cggtcgatcc
     2161 gcccggactg accgcctcgg acgtcacgcc gcacggatgc ccctggtcct cccgggggca
     2221 tcggcatgtc tggcgggcga cgaggccgag cgacgacgct agatccggaa cgcctcgccg
     2281 cgtcggaagc gctcgcggtg tcgaccgcgc cagatcgtga cccggccgcg gccgtgcacc
     2341 agccacgcgt tgttgcagcc gaccgccgcc gtgtcctcgt cgatgcccag gatcacgcct
     2401 ccgcgcgggg cctggagggc cacgaaggcc gagaacggct cgggccaggc gtcgtagtgg
     2461 ggcaggacgg ccacgccatc gaccagcgcc aggccgatcg gccagcgcac cgggaacggc
     2521 atcggcatcc ggaccttggg caggaagcgg aagtctacgg tccggccgac gatggccatc
     2581 gccccagcgg agcagccggc gatcaccgcg ccccggtcgt tggcggcgcg gatcgcgccg
     2641 cccagcggcg actgctgcaa cacgtccagc aggtgcttcg gatggccgcc ggagagatag
     2701 atgagatccg cctcgccgat cgcctggagt gccgccccgt cgaacccctc cgcggcgtta
     2761 cgcaccagca cgggttcgac ctccgcaccg agcgcgctga agtgctcgat gcccatctcg
     2821 gcccagtcgc ggaagatctg ctcgccgtcg ggcgcggcgg ccgtcggcag gacgacgacg
     2881 cgcggcctcg gacggccggt ggcttcgagc aacccgcggt cgaactctgc catcggcgcg
     2941 aggaactctc cggcgcccac gagcgccacg ggtccgggca cggccgcagc atagatcagg
     3001 ccgccgaagt gcgatctgga cgccccgatc cgccgcctga cacacttcgg gggatgacag
     3061 ccacaagcgg ccgcggcgga ccccgggact gggacgccac gacgtacgac caggtcgccg
     3121 cccccatgac ccggtggggc tccgacgtcc tcgagcggct gccgctgcgc ggcgacgaga
     3181 cggtgctcga cgcgggctgc gggacgggcc agatcaccga gcagctcgtg gagcgcctcc
     3241 cgcgcggccg cgtcgtggcg ctggacggct caccggcgat ggtcgaggcc gcccgcggcc
     3301 ggctggctcg cttcggcgaa cgggtggact tcgtcatcgc ggacctcgcg gcgccgctgc
     3361 ccctcgccga cgcgagcgtc gacgcgatcc tctcgaccgc cacgttccac tgggtcgcgg
     3421 accacggggc cctgttccgg cacctcggcc gcgtcgtcgc tcccggcggg cggctggtcg
     3481 cccagtgcgg gggcgagggc aacatcgtgc agctgcgcgc tgcgctcgac gaggtcggcg
     3541 acgggtggga gggcccctgg aacttcgcgt cgccggagga aacgcggcgg cggctcgagg
     3601 cggccggttt cgtcgggatc gacacctggc tcaccgacga gccgacgtcg ttcgagccgg
     3661 gtcccgcgtt ccgcgactac ctccggacgg tggccctgag cgcgcacgtc gcgcggctcg
     3721 agcccgaagc gcaggacgac ttcctcgacg ccgtgatcga gcgcct
//
LOCUS       DBNHXO010000065         1819 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4382523, whole genome shotgun sequence.
ACCESSION   DBNHXO010000065 DBNHXO010000000
VERSION     DBNHXO010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4382523"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1819
                     /locus_tag="AC162A_01480"
     CDS             <1..>1819
                     /locus_tag="AC162A_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR5009534.1"
                     /translation="ANGELIQNAFRIGLLRMERVVRERMTIQEIDKATPNALINIRPV
                     VAAMKEFFGGSQLSQFMDQTNPLAELTSKRRLSALGPGGLSRERAGFDVRDVHHSHYG
                     RICPIETPEGPNIGLIGSLATYGRINAYGFIETPYRRVKRTVAHDDAQLSTFAAGADL
                     IGKDGKVILKKGEAFNDAAHKELARQKALAFPIRPVVTDEIDYLPADEEEEHHVAQAN
                     APLDAENHFIGERVPSRYRDTFPEARPDQIEYMDVSPKQVVSVATALIPFLEHDDANR
                     ALMGSNMQRQAVPLLEPEAPIVGTGMEARAARDSGQVVIARRAGVVTSVTAERVQVEA
                     DSGELDEYRLLKFVRSNQGTCINQRPIVTVGQRVDSGDAIADSSSTDGGELALGRNVL
                     VAFMSWEGGNYEDAIVISERLVRDDLFTSIHIEKHEIESRDTKLGPEEITRDIPNVGE
                     EALKDLDEEGIVYIGAEVRPGDILVGKITPKGETELTAEERLLRAIFGEKAREVKDSS
                     LRLPHGERGKVVEIREFRREWNDDLQPGVNRLVRVSVAQKRKVSVGDKMAGRHGNKGV
                     IAKVLPMEDMPFLPDGSSVDILLNPLGVPSRMNIGQILET"
BASE COUNT          327 a          655 c          607 g          230 t
ORIGIN      
        1 gcgccaacgg cgagctcatc cagaacgcgt tccggatcgg cctcctgcgc atggagcgcg
       61 tcgtccgcga gcggatgacg atccaggaga tcgacaaggc cacgcccaac gccctcatca
      121 acatccgtcc ggtcgtggcg gcgatgaagg agttcttcgg cggcagccag ctcagccagt
      181 tcatggacca gacgaacccg ctcgcggagc tgacgtccaa gcggcgcctg tccgccctcg
      241 gcccgggcgg cctgtcgcgc gagcgcgccg ggttcgacgt ccgtgacgtg caccacagcc
      301 actacggccg gatctgcccg atcgagaccc ccgagggccc gaacatcggc ctgatcggct
      361 cgctcgcgac ctacggccgg atcaacgcct acggcttcat cgagacgccc taccgccggg
      421 tcaagcggac cgtcgcccac gacgacgcgc agctgtcgac ctttgccgcc ggcgccgacc
      481 tgatcggcaa ggacggcaag gtgatcctca agaagggcga ggcgttcaac gacgcagcgc
      541 acaaggagct cgcccgccag aaggcgctcg cgttcccgat ccgccccgtc gtcaccgacg
      601 agatcgacta tctcccggcc gacgaggaag aggagcacca cgtcgcgcag gccaacgcgc
      661 cgctcgacgc cgagaaccac ttcatcggcg agcgcgtccc gtcccgctac cgggacacct
      721 tccccgaggc gcggccggac cagatcgagt acatggacgt cagccccaag caggtcgtct
      781 cggtcgcgac ggcgctgatc ccgttcctcg agcacgacga cgccaaccgt gccctcatgg
      841 gctcgaacat gcagcgccag gcggtgccgc tcctcgagcc cgaggcgccg atcgtcggca
      901 ccggcatgga ggcccgcgcc gcgcgcgact ccggccaggt cgtgatcgcc cgccgcgcgg
      961 gcgtcgtgac cagcgtgacc gccgagcgcg tccaggtcga ggccgactcg ggcgagctcg
     1021 acgagtaccg gctgctcaag ttcgtgcgca gcaaccaggg cacgtgcatc aaccagcgcc
     1081 cgatcgtgac ggtcgggcag cgggtcgaca gcggcgacgc catcgccgac tcgtcctcga
     1141 ccgacggcgg cgagctcgcc ctgggccgca acgtcctggt cgcgttcatg agctgggagg
     1201 gcggcaacta cgaggacgcg atcgtgatca gcgagcgcct cgtgcgcgac gacctgttca
     1261 cgagcatcca catcgagaag cacgagatcg agtcgcggga caccaagctg ggccccgagg
     1321 agatcacccg ggacatcccc aacgtcggcg aggaggccct caaggacctc gacgaggagg
     1381 ggatcgtcta catcggcgca gaggtccgcc ccggcgacat cctggtcggc aagatcacgc
     1441 ccaagggcga gaccgagctg accgcggagg agcgcctgct gcgcgcgatc ttcggggaga
     1501 aggcgcgcga ggtgaaggac tcctcgctcc ggttgccgca cggcgagcgg ggcaaggtcg
     1561 tcgagatccg cgagttccgc cgcgagtgga acgacgacct ccagccgggc gtcaaccgcc
     1621 tggtccgggt gtcggtcgcc cagaagcgca aggtctcggt cggcgacaag atggccggcc
     1681 gccacggcaa caagggcgtc atcgccaagg tcctgccgat ggaagacatg cccttcctgc
     1741 ccgacggcag ctcggtggac atcctgctca acccgctcgg cgtgccgagc cgcatgaaca
     1801 tcggtcagat cctcgagac
//
LOCUS       DBNHXO010000066         6652 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1009824, whole genome shotgun sequence.
ACCESSION   DBNHXO010000066 DBNHXO010000000
VERSION     DBNHXO010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1009824"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..934)
                     /gene="prmC"
                     /locus_tag="AC162A_01485"
     CDS             complement(20..934)
                     /gene="prmC"
                     /locus_tag="AC162A_01485"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR5009535.1"
                     /translation="MADAAVTVSELVRVAAERISASGSGSARLDAELLLAQTLGIDRT
                     GVLAHPDAPVGPDARQAFEVAVKRRERGEPVAYIRGFREFHGLAFATDSRALIPRPET
                     ELLVDAAIDEIVARLTATPRLPGAPALRVVDVGTGTGAIAVSVLAALRRRKMADQVMV
                     IAIDVSEEALQLARENAVGHGVADRMVFVTADLLPHHVEPPYAVVCANLPYVPTGDLP
                     ALSPELAYEPRAALDGGPDGLDVLRRLLDRLTEITEPDGIALLEIGAEQGEAVAAEVA
                     GRLPGARCTVMPDLAGRPRLVRIELSPA"
     gene            complement(941..3214)
                     /locus_tag="AC162A_01490"
     CDS             complement(941..3214)
                     /locus_tag="AC162A_01490"
                     /EC_number="3.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF046269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HIR5009536.1"
                     /translation="MADSPVGAEVTRFIDPVARPIVKATDLGSVQVLKQGNVYLLTDP
                     FGDVHADSRGLGLYDGDTRRLSSSILRINGERPVLLQASAGGNYHGAIQLTNPRLERN
                     VADKVRPEDALASQKLGIARHRVIAGDSLEERVTIVNYAEHDEQVEVTLELAGDAADI
                     FEVRGWTRAARGRQLPIAARADRVTFRYEGLDGRRTATHVAFGEPADAVEAVDPEVAG
                     TVNAGWVRFTWHWPMASGEARELHWVAWAIERPVTGATSDVETDDALFPPEPLVAVDE
                     VAASYHAWSRGVSEIKTDNELVNLAIARSTSDLRLLVNDGPGEAERYLAAGIPWFATL
                     FGRDAIIASFQALCVRPQVAVETLEVLARLQATDEDPERDAEPGKILHELRTGEMART
                     GELPFRPYYGSVDATPLWLILFAATFDWTGDMTLVDRLWPNAMRALDWIDTYGDRDGD
                     GFIEYQRRAPSGLLNQGWKDSSDGIRDRNGKLAPTPIALAEVQGYVFDAKLRLATLAR
                     LRGDEALAVRLEADAERLRGRFEEAFWSDDQAFYAMALDGDKRRADAIASNAGHCLWS
                     GIASPDRARRVIDRLMAPDMFSGWGIRTYAAGQPGYNPIGYHSGTVWPHDVSLIAAGF
                     KRYGRHEDANRLVGRMFEAAQHFADFRLPELFCGFDRDHSPVPVPYPVACSPQAWAAG
                     SLFLFLETMLGLRPHADRRELELARPELPEWLQKVTVNNLRVGEGTVDLLFHRWRGGT
                     SAEVLRKSRDLDVTIRM"
     gene            complement(3217..4311)
                     /gene="prfA"
                     /locus_tag="AC162A_01495"
     CDS             complement(3217..4311)
                     /gene="prfA"
                     /locus_tag="AC162A_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR5009537.1"
                     /translation="MTDDHGLDAKLEELARQYDDVSEQLASPEVHADPGALRRLGREH
                     ARLEPVVAAYRELTDVREQLVGARHMRDADQDDEVKAMAREEIATLEARETQLIDDLR
                     VSLLPRDPNDDRNVIVEIRAGAGGEEAALFATELLRMYSRYAQEHRFATDLMSLNETG
                     IGGTKEAILEVAGDGAYSRLKFEGGVHRVQRVPSTESSGRIHTSTATVIVLPEVDDVE
                     VEIDEARDLRIDVKRSSGPGGQSVNTTDSAVRITHLPTGLVVEIQDEKSQHKNKAKAM
                     SVLRSRLYDLELAKQREAQSAERKVMVGGGDRSEKIRTYNFPDNRVTDHRIGVTIHNV
                     PGVLEGQLDPLIDALVMADQADRLSHLTET"
     gene            complement(4337..5335)
                     /locus_tag="AC162A_01500"
     CDS             complement(4337..5335)
                     /locus_tag="AC162A_01500"
                     /inference="COORDINATES: protein motif:HMM:NF018798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HIR5009538.1"
                     /translation="MPRFNYGGQALIEGVLMRGRDAIAVALRHPDGRIVFATERLDSG
                     FHGNRLSRAPFVRGLVVLYETLIVGTRWLIRSAGLQAEDEGIELGKGSVALMLGITAI
                     LGIGLFFLLPLIVATFTVGNADNGLVQHAVEGLIRVGIFIGYLALIARTPDIARVFQY
                     HGAEHMTIHALEHDDPLDPEHIRKYPTAHPRCGTEFLVVVILLSILAFSLVGRQAPLV
                     MIASRILLIPVIASVGYELLRLGARHRSNPIVRIVMWPGILVQMITTKQPTDDMIEIA
                     IVAMEEAIRADGSEVPANSLELERYPMPLPGETAAGVRAAAELTDEPASAEPPLPG"
     gene            5417..6067
                     /locus_tag="AC162A_01505"
     CDS             5417..6067
                     /locus_tag="AC162A_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018787705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5009539.1"
                     /translation="MRVVIAEDSVLLREGLVRLLEDAGASVVARVGDGPSLVAAVVEQ
                     RPDVSIVDVRMPPTQRDEGLRAAIEARRLVPGSPVLVLSQYVEREYASELLADGAGGV
                     GYLLKDRVADVRDFMDALARVAGGGTALDPEVVAQLMVSHRRDDRLAALTPREREVLA
                     AMAEGKTNMGIATALVISEGAAEKHITSILGKLDLPDSENDHRRVLAVLAFLDAGG"
     gene            complement(6327..>6652)
                     /locus_tag="AC162A_01510"
     CDS             complement(6327..>6652)
                     /locus_tag="AC162A_01510"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014981246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5009540.1"
                     /translation="PTALISTLPVGERLPPAIERSAYFVVVEALANVAKHSGASRCEV
                     ALRKDPWALVIEVRDDGAGGATVAPGGGLAGLRDRAQALDGTLAVSSPTGGPTVVRVE
                     LPIPS"
BASE COUNT          885 a         2370 c         2441 g          956 t
ORIGIN      
        1 cgcgatcggg cggccccgct cacgcgggcg acagctcgat gcggacgagc cgtggccggc
       61 cggcgaggtc gggcatgacc gtgcagcgcg ccccgggcag ccgcccggcc acttcggcgg
      121 cgacggcctc accctgctcg gccccgatct cgagcagcgc gatcccgtcg ggctccgtga
      181 tctccgtcag ccggtcgagc agccggcgca gcacgtcgag gccgtcggga ccgccgtcga
      241 gcgcagcccg cggctcgtag gcgagctcgg gcgagagggc tggcaggtcg cctgtcggca
      301 catacgggag gttcgcgcac acgaccgcgt agggcggctc gacgtggtgc gggagcagat
      361 cggccgtgac gaacaccatc cggtcggcga ccccgtgccc gacggcgttc tcgcgggcga
      421 gctggagcgc ctcctcggac acgtcgatcg cgatcaccat gacctggtcg gccatcttcc
      481 ggcgtcgcag cgcggccagc accgatacgg cgatcgcccc cgtccccgtc cccacgtcga
      541 ccacccgcag tgccggcgcc ccgggcagcc ggggggtcgc cgtgaggcgg gcgacgatct
      601 cgtcgatggc cgcgtcgacc agcagctcgg tctcgggccg cgggatcagc gcgcgggagt
      661 ccgtcgcgaa cgcgagcccg tggaactcgc ggaagccccg gatgtacgcg acgggctcgc
      721 cccgctcgcg gcgcttgacg gcgacctcga acgcctgccg cgcgtccggg ccgaccgggg
      781 cgtcgggatg ggcgaggacg ccggtgcggt cgatccccag cgtctgggcc agcaggagct
      841 ccgcgtcgag gcgggccgac cccgagccgg atgccgagat ccgctcggcc gcgacgcgga
      901 cgagctcgga cacggtgacg gcggcgtcgg ccatcgaccc ctacatccgg atggtgacgt
      961 cgaggtcgcg gctcttgcgc aggacctccg cggacgtgcc gccgcgccac cggtggaaca
     1021 ggaggtcgac ggtgccctcg ccgacccgca ggttgttgac ggtcaccttc tgcagccact
     1081 cgggcagctc cgggcgcgcc agctcgagct cgcgccggtc ggcgtgcggg cgcagcccga
     1141 gcatcgtctc gaggaacagg aacagcgacc cggcggccca ggcctgcggc gaacacgcga
     1201 cggggtacgg gacggggacc ggcgagtggt cgcggtcgaa gccgcagaac agctccggca
     1261 gccggaagtc cgcgaagtgc tgggcggcct cgaacatccg cccgacaagg cggttggcgt
     1321 cctcgtgccg gccatagcgc ttgaacccgg ccgcgatcag cgagacgtcg tgcggccaga
     1381 cggtgccgga gtggtagccg atcgggttgt agccgggctg cccggcggcg tacgtgcgga
     1441 tcccccagcc gctgaacatg tcgggtgcca tcaggcggtc gatcacccgc cgcgcacggt
     1501 cgggcgaggc gatcccggac cacaggcagt ggcccgcgtt ggaggcgatc gcgtccgcgc
     1561 ggcgcttgtc gccgtcgagg gccatcgcgt agaaggcctg atcgtcggac cagaacgcct
     1621 cctcgaaccg cccccggagc cgctccgcgt cagcctcgag gcgaacggcg agggcctcgt
     1681 ccccgcgcag ccgagccagc gtcgccagcc gcagcttggc atcgaacacg tacccctgga
     1741 cctcggccag cgcgatcggc gtcggggcga gctttccgtt gcggtcccgg atcccgtcgc
     1801 tcgagtcctt ccagccctgg ttcagcaggc cgctcggcgc gcgtcgctgg tactcgatga
     1861 acccgtcgcc gtcgcggtcg ccataggtgt cgatccagtc gagcgcacgc atcgcgttgg
     1921 gccacagccg gtcgacgagg gtcatgtcgc ccgtccagtc gaacgtcgcg gcgaacagga
     1981 tcagccacag cggcgtcgcg tcgacggatc cgtagtaggg ccggaacggc agctcacccg
     2041 tacgcgccat ctcgcccgtg cgcagctcgt gcaggatctt gccgggctcg gcgtcgcgct
     2101 ccgggtcctc gtcggtggcc tggagcctcg ccagcacctc cagcgtctcg accgcgacct
     2161 ggggccggac gcacagcgcc tggaacgacg cgatgatcgc gtcccggccg aacagcgtcg
     2221 cgaaccacgg gatgcccgcc gcgaggtagc gctcggcctc gccgggcccg tcgttgacca
     2281 gcagccgcag gtcgctcgtg gagcgggcga tcgcgaggtt gacgagctcg ttgtcggtct
     2341 tgatctccga tacgccccgg ctccacgcgt ggtatgaggc ggcgacctcg tcgaccgcga
     2401 caagcggctc cggcgggaac agggcgtcgt cggtctcgac gtcggacgtg gctccagtca
     2461 cgggacgctc gatcgcccag gcgacccagt gcagctcccg tgcctccccc gaggccatcg
     2521 gccagtgcca ggtgaaccgc acccagcccg cgttgacggt gccggcgacc tcggggtcga
     2581 ccgcctcgac cgcgtcggcc ggctcgccga aggcgacgtg ggtcgcggtg cgccggccgt
     2641 ccaggccctc gtagcggaac gtgacgcggt cggcgcgggc cgcgatcggc agctggcggc
     2701 cccgggcggc gcgcgtccag ccgcgaacct cgaagatgtc ggcggcgtcc cccgccagct
     2761 cgagcgtcac ctcgacctgc tcgtcgtgct ccgcgtagtt cacgatcgtg acccgctcct
     2821 cgagcgaatc ccccgcgatg acgcggtgcc gcgcgatgcc cagcttctgc gaggcgaggg
     2881 cgtcctctgg gcgcaccttg tcggcgacgt tccgctcgag gcgcgggttg gtcagctgga
     2941 tggccccgtg gtagttgcca ccggccgacg cctggagcag caccgggcgc tcgccgttga
     3001 tgcgcaggat cgaggacgag aggcgccggg tgtcgccgtc gtagaggccg aggccgcggc
     3061 tgtcggcgtg gacgtcgccg aacgggtctg tcagcaggta gacgttgccc tgcttgagca
     3121 cctggaccga ccccaggtcg gtcgccttga cgatcgggcg cgcgaccggg tcgatgaagc
     3181 gggtcacctc cgcccccacc ggggagtcgg ccaccgtcag gtctccgtga ggtggctcag
     3241 gcgatcggcc tgatcggcca tgacgagggc gtcgatgagc gggtcgagct ggccctcgag
     3301 gacccccggc acgttgtgga tcgtgacccc gatgcggtgg tccgtgaccc ggttgtcggg
     3361 gaagttgtag gtgcggatct tctccgaccg atcgccgccg ccgaccatca ccttgcgttc
     3421 cgcgctctgc gcctctcgct gcttggcgag ctcgaggtcg tacagccggg agcgcagcac
     3481 cgacatcgcc ttggccttgt tcttgtgctg gctcttctcg tcctggatct cgaccaccag
     3541 ccccgtcggc aggtgggtga tccggaccgc ggagtccgtg gtgttgaccg actggccgcc
     3601 cggcccggag ctgcgcttga cgtcgatccg gaggtccctc gcctcgtcga tctcgacctc
     3661 gacgtcgtcg acctcgggca ggacgatcac cgtggcggtg ctcgtgtgga tccggccgct
     3721 cgactcggtc gacggcacgc gctgcacgcg gtggaccccg ccctcgaact tgagccggct
     3781 gtaggcgccg tcgccggcga cctcgaggat cgcctccttg gtcccgccga tgcccgtctc
     3841 gttgaggctc atgaggtccg tcgcgaaccg gtgctcctgc gcgtagcggc tgtacatccg
     3901 cagcagctcg gtcgcgaaca gcgccgcctc ctcgccaccc gcgcccgccc ggatctcgac
     3961 gataacgttg cgatcgtcgt tggggtcgcg cgggagcagc gagacgcgga ggtcgtcgat
     4021 cagctgcgtc tcgcgggcct cgagcgtcgc gatctcctcg cgcgccatgg ccttgacctc
     4081 gtcgtcctgg tccgcgtcgc gcatgtggcg cgcgcccacg agctgctcgc ggacgtcggt
     4141 gagctcgcgg tacgcggcga cgaccggctc gaggcgcgcg tgctcgcggc ccagccggcg
     4201 cagggcgccc ggatcggcgt gcacctcggg gctggcgagc tgctccgaca cgtcgtcgta
     4261 ctggcgcgcg agctcttcca gcttggcgtc gaggccgtgg tcgtcggtca tggtcgtgct
     4321 cagcatcccg ggccggtcag cccggcagcg ggggttcggc ggacgccggc tcgtcggtca
     4381 gctcggccgc ggcgcgcacg cccgcggcgg tctcacccgg caacggcatc gggtagcgct
     4441 cgagctcgag gctgttcgcc gggacctcgg agccgtcggc gcggatcgcc tcctccatcg
     4501 ccacgatcgc gatctcgatc atgtcgtcgg tcggctgctt ggtcgtgatc atctggacga
     4561 ggatcccggg ccacatcacg atccgcacga tcgggttgga ccggtggcgg gcgccgaggc
     4621 ggagcagctc gtagccgacc gaggcgatca ccgggatcag gaggatccgg ctggcgatca
     4681 tgaccagcgg cgcctgacgg ccgacgaggc tgaaggccag gatcgagagc aggatcacga
     4741 ccaccaggaa ctccgtgccg cagcgcgggt gggcggttgg gtacttgcgg atgtgctcgg
     4801 gatcgagcgg gtcgtcgtgc tcgagcgcgt ggatcgtcat gtgctcggcg ccgtggtact
     4861 ggaacacgcg cgcaatgtcg ggcgtgcgcg caatgagcgc gaggtagccg atgaagatcc
     4921 cgacccggat caggccctcg accgcgtgct ggacgaggcc gttgtcggcg ttgcccacgg
     4981 tgaacgtcgc gacgatgagc ggcaggagga agaacagccc gatgcccagg atggcggtaa
     5041 tgccgagcat cagcgccacg ctgcccttgc ccagctcgat gccctcgtct tcggcctgga
     5101 ggcccgcgga gcggatcagc cagcgggtgc cgacgatcag cgtctcgtac aggaccacga
     5161 ggccgcgcac gaacggggcg cgcgacagcc ggttgccgtg gaagcccgaa tcgaggcgct
     5221 cggtcgcgaa cacgatccgg ccgtcggggt gacggagcgc gacggcgatg gcgtcgcggc
     5281 cgcgcatcaa gacgccctcg atcagcgcct ggccgccgta gttgaagcgg ggcatgcggc
     5341 cagtctaccg ggtgggtccc gctccgcccc ccggtccttt cggggggtgc atcatcgggg
     5401 cgtgaacggc gccgggctgc gggtggtcat cgcggaggac tcggtcctcc tgcgcgaggg
     5461 gctcgtccgc ctgctcgagg acgccggggc gtcggtcgtg gcgcgggtcg gcgacggccc
     5521 gtcgctggtg gccgcggtcg tcgagcagcg accggacgtg tcgatcgtcg acgtgcggat
     5581 gccgcccacc cagcgcgacg aggggctgcg cgccgcgatc gaggcgcggc ggctcgtgcc
     5641 cggctcaccg gtcctcgtgc tcagccagta cgtcgagcgc gagtacgcga gcgagctgct
     5701 cgcggacggg gccggcgggg tgggctacct gctcaaggac cgggtcgcgg acgtgcgcga
     5761 cttcatggac gcgctcgcgc gcgtggcggg cggcggcacc gcgctcgatc ccgaggtcgt
     5821 tgcgcagctc atggtcagcc accggcgcga cgatcggctc gccgccctga cgccgcgcga
     5881 gcgcgaggtg ctagccgcga tggccgaggg caagacgaac atgggcatcg cgaccgcgct
     5941 ggtgatcagc gagggtgccg ccgagaagca catcacgagc atcctgggca agctcgacct
     6001 gcccgactcc gagaacgacc accggcgcgt gctcgccgtg ctcgccttcc tcgacgcggg
     6061 cggctagttg tagtccgtcg cgggcctggc tcgggccggg cggtcgccac gcggctggat
     6121 gtgcccagga tcgaccaaat cgctccatat cggccccgag ttccggcctc gttggccctt
     6181 cgcgccgatc ttgtgcctgc gagtcacgga tttcgggccg aagcacgcct cggatcgccg
     6241 gatggagcat tttggcgggt accaggcaca acggcgacac gccgagactg gaggtcccgc
     6301 ccgcgggact cgcgctcgcc gctcgcctac gacgggatcg gcagctcgac gcggaccacc
     6361 gtgggaccac cggtcgggct cgagaccgcg agcgttccgt ccagcgcctg cgcccggtcg
     6421 cgcagccccg ccaggccgcc tccgggtgcg accgtcgcgc cgccggcgcc gtcgtcgcgc
     6481 acctcgatga ccagcgccca cgggtccttg cgcagcgcca cctcgcaccg gctcgccccg
     6541 ctgtgcttcg cgacgttggc cagcgcctcc acgacgacga agtacgcaga gcgctcgatc
     6601 gccggcggga gccgctcgcc aacgggcagc gtgctgatca gcgcggtcgg ca
//
LOCUS       DBNHXO010000067         1894 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1009935, whole genome shotgun sequence.
ACCESSION   DBNHXO010000067 DBNHXO010000000
VERSION     DBNHXO010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1009935"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..738
                     /locus_tag="AC162A_01515"
     CDS             190..738
                     /locus_tag="AC162A_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013540.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5009541.1"
                     /translation="MTTENGRAFNEWLRAQLKAKKMSQRQLAQQSGVDHSTISRLIRG
                     DRMPSLGTATKLARGLREIRDDSDTSTALGVVSMGPQNPTARVEYALRADEALSEPQV
                     RQIMEYYLAVRVRRFARTYSSVGSAESGLNGNGSTSSGSVYGRQSSVAGVAAAPAVRA
                     LPPRYGTQRPPARPPTRTPGRP"
     gene            complement(1169..>1894)
                     /locus_tag="AC162A_01520"
     CDS             complement(1169..>1894)
                     /locus_tag="AC162A_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009542.1"
                     /translation="LTDRLVLSLVAVAVACTLAAASVVVARGASRRAAVWLAGSVLWA
                     VVALALFAPSLAVITPGLPNDHYHSFLDPLVLALVAAGLAHVAGVGVSRASSPVRPIM
                     AAGAGVLLVAVSVLAWPPAVSPDGGWRLADQAAGRAAAAVDNGRFVLEGIPPFKNANA
                     MRFPLEHRGAAPFPADEGIPVPPTLPVVVVCDPLFDDVMGAACGGPAEEAWAAERGVP
                     TLEAIDSFEAGPRRVITVYRASP"
BASE COUNT          302 a          700 c          681 g          211 t
ORIGIN      
        1 gggggtaccg gcggagggca acccgatcgg gtcacgcctg caccgcacgc gacctcacca
       61 gacctcccgg gcccgcacgc ggcaccgtgc aagcgccgag tacgaacggg tgatgggacg
      121 gggagaccgc tcacccgaca ctccagttga tcccgcgcac cgggcgcagg agcttagccc
      181 aaggcgagca tgacgaccga gaacggccgc gcgttcaacg aatggctgcg agcacagctc
      241 aaggccaaga agatgtcgca gcgccagctg gcgcagcagt caggcgtcga ccactcgacg
      301 atctcccgac tgatccgggg cgaccggatg ccctccctcg gtacggcaac gaagctcgcc
      361 cgcggcctcc gcgagatccg cgacgactcc gacacgtcca ccgcgctcgg cgtggtgtcc
      421 atggggcccc agaacccgac ggcgcgtgtc gaatacgcgc tccgggcgga cgaggcgctc
      481 tcggagccgc aggtccggca gatcatggag tactacctgg cagttcgggt gcgccggttc
      541 gcgcggacct actccagcgt cgggtccgcc gagtcgggct tgaacggcaa cggctccacg
      601 agctccggct ccgtctacgg ccggcagtcc tcggtggccg gtgtggccgc ggcccccgcg
      661 gtccgcgccc tgccgccgcg ctacggcaca cagcgaccgc ccgcgcggcc tccgacccgc
      721 acgccgggtc gaccatagaa cacctcaagg gcacggcctg aacgtccctc cacgttcggg
      781 tctcggggac agcgaacgcc ggtctgcagg ggccggcgtt cgcgcttaag ccggatcgcc
      841 gccccgccgc ccctcacgtc ccgccgcccg ccgcccgccg cccgccggtt tcgtgcccgt
      901 gctgcacatc ggcggcccgg gcgtgcgccc agggcacatt cgcgaccttg cggcgaccga
      961 gagtgtcggt gccgtgcgcc ggacgcacaa cggcgtgtct cagtacggcc cttcgcccac
     1021 accgacgtcg gactcgtgcg acgaccgcac aggattgacg cgcagccgtc tcgacggcga
     1081 cctcggcggg ctggaatgtg catgtggcgc acagcgagtt gcctggcacc gacggccgga
     1141 gtcggcggga acctggaggt ggctcaggct agggcgacgc gcgatagacc gtgatcacgc
     1201 gacggggccc ggcctcgaag ctgtcgatcg cctcgagggt cggcacgccg cgctcagccg
     1261 cccaggcttc ctccgccggg ccgccgcagg cggcgcccat gacgtcgtcg aagagggggt
     1321 cgcagacgac gaccaccggc agggtcggcg ggacggggat gccctcgtcg gccgggaatg
     1381 gcgcggcgcc acggtgctcg agcgggaagc gcatcgcgtt cgcgttcttg aacggcggga
     1441 tgccctcgag cacgaaccga ccgttgtcca ctgctgcagc tgcgcgcccc gcggcctgat
     1501 cggcgagccg ccagccgccg tcgggtgaga ccgccggcgg ccaggcaagg acggacacgg
     1561 cgacgagcag cacacccgcg cccgcggcca tgatcggacg gacgggactc gacgcccggc
     1621 tgacgccgac tccggcgacg tgcgccagcc cggcggcgac caacgcgagc acgagcggat
     1681 cgaggaacga gtggtagtgg tcgttcggga ggcccggcgt gatgaccgcg aggctcgggg
     1741 cgaacaaggc gagcgcgacg accgcccaca gcaccgaacc ggcgagccag accgcggccc
     1801 gccgcgacgc gccgcgcgcg accacgacgg atgccgcggc gagcgtgcac gcgacggcga
     1861 cagccacgag cgacaggacg agtcgatcgg tgag
//
LOCUS       DBNHXO010000068         4659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1208506, whole genome shotgun sequence.
ACCESSION   DBNHXO010000068 DBNHXO010000000
VERSION     DBNHXO010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1208506"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="AC162A_01525"
     CDS             complement(<1..542)
                     /locus_tag="AC162A_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009543.1"
                     /translation="MAPRFVAVAEVADGALTRLSTEVATLARTLAEASGGTAVGLVVD
                     ADPAPAAAELAGFMAQVIAVSAPATANEAWAPHAAAEVGRLIDDGATHVLAGASTDGR
                     DLAGMVVGRWTWGLLANATAVTWGGDGLVAEATVLGGKAITRSAFTGAAGVVTVRPNA
                     TTAEPAAAAGSVEPRAAAAGA"
     gene            complement(542..1339)
                     /locus_tag="AC162A_01530"
     CDS             complement(542..1339)
                     /locus_tag="AC162A_01530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR5009544.1"
                     /translation="MQILVLTKPVPDPAAGAERLSGPDFRLDRSASPTVVNGNDEYAL
                     EAALQLVEAHGGEVAILAMAPANATETMRKALAMGAQRGVLVTDPALAGADLPATVRV
                     LAAAAAAETFDLLLTGLDTSDGTGGAVAAGIAARLGLPLLSAAAHIEPDPAAGRVRVK
                     RLSAKGYDLIEAPMPAVISCTQALGAPRYPSLKGIMAARQREIAVRSLADLGNPDDGP
                     LLPDGGWATRVVAADPPPARDTGRVVKAPAADAAREIADFLADRRLI"
     gene            complement(1386..2564)
                     /locus_tag="AC162A_01535"
     CDS             complement(1386..2564)
                     /locus_tag="AC162A_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5009545.1"
                     /translation="MTTSATAHGSIRLTEDQVLLRDAVRELADEQIAPRAAEIDRTAE
                     WPDDVRRLLASHDILAIPFAERHGGLGADLLTLCLAIEQISRACATSGLILAVQSLGA
                     IPIQVAGTPEQQDRWLPGLASGEKLIAFALTEADAGSDPSGIRTRAVIDGDDYVIDGG
                     KRFISHASVADYVVVFAVTNPDATRQSRRLSAILVETDRPGFLVTRLEHKMGIRGSPT
                     AELAFDGLRVPISNRLGDEGAGFAIAMQTFERSRPGIAAQAVGIAQGALEAATRHATE
                     RRQFGAPIGELQMVQAMLADMDASTEAARQLLYTACQAIEAGAPDAARWAAICKLVAG
                     DAAMRVTTDAVQVFGGYGYVDEYPVERMMRDAKITQLYEGTQQIQRLIVARALLAQRP
                     "
     gene            2716..2940
                     /locus_tag="AC162A_01540"
     CDS             2716..2940
                     /locus_tag="AC162A_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009546.1"
                     /translation="MDQSYLLLLGIAILVAIGSGARIRNRVLTERADAAQTESPFAVS
                     TEGDKICPKCGMGNLWTERRCSACGGALKG"
     gene            complement(2944..>4659)
                     /locus_tag="AC162A_01545"
     CDS             complement(2944..>4659)
                     /locus_tag="AC162A_01545"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR5009547.1"
                     /translation="VTKPKRLTFNRDALLILGMIGGVVATELLAQVFQVAAYGVIPGA
                     FIANAAGTALSGGDQAALEAVFAILWWAHMVLVAAFLCYLPFSKHLHIATAIPNIALR
                     KLRPRGELPAMDLEAEDATFGIRTVADLSWKDLLDGFTCTECGRCQEACPAWNTGKPL
                     NPKSFIMGIRHEAVDAEAGLPLIPNSPSVRAIRPGLDDRLPADALARPLVDTAIPFDA
                     VWDCLTCGACVEACPVTIEHVDKIVGIRRNLVLEESRFPQELTAAFKGMESVANPWGQ
                     PPSSRLDWAKGLPFEVRTVAAVQAAGELGDLEVLYWVGCAAAFDDRNRRVARAVATCL
                     FEAGVSFAVLGQEESCTGDPARRMGNEYVFQILASQNVDTLNRYRMGERTIVTACPHC
                     FNTIGNEYGQLGGSFTIRHHSEYLSSLVSSGRLQLGADGLPGRTVTYHDACYLARYNG
                     VIAPPREVLGAIPGVELREMENSGRQTFCCGAGGGRMWMEESRGTRVNAARTQQALAT
                     GAETVATACPFCMVMLRDGLNDAGRGAGSEDQVTAQDISELLAAAIVPFGGREIPEGR
                     RLPVL"
BASE COUNT          629 a         1691 c         1699 g          640 t
ORIGIN      
        1 gcaccggcag ccgcagcccg cggctcgacg gacccggccg ccgcggcggg ctccgctgtc
       61 gtcgcgttcg gccggacggt gaccacgccc gccgcaccgg tgaacgcgct gcgggtgatg
      121 gccttgcccc cgaggacggt cgcctccgcc acgagcccgt cgccgcccca cgtcaccgcg
      181 gtcgcgttcg cgagcaggcc ccatgtccat cggccgacga ccatcccggc gaggtcgcgt
      241 ccatccgtgg aggcgccagc caggacgtgg gtcgccccgt cgtcgatcag ccgcccgacc
      301 tcggcggccg cgtgcggggc ccacgcctcg ttcgccgtcg ccggcgcgct caccgcgatg
      361 acctgggcca tgaacccggc gagctctgca gccgcggggg caggatccgc gtccacgacc
      421 aggcccacgg ccgtgccgcc ggacgcctcc gcgagggttc tcgcgagcgt cgcgacctcc
      481 gtcgagaggc gggtcagcgc accgtcggcg acctccgcga cggccacgaa ccgcggcgcc
      541 atcagatcag cctccggtcg gcgaggaagt cggcgatctc gcgggccgcg tcggctgccg
      601 gcgctttcac gacgcggcct gtgtcccggg cgggcggcgg atcggcggcg acgacgcgcg
      661 ttgcccagcc accatcgggg agcagcgggc catcgtccgg attcccgagg tcggccagcg
      721 agcgcaccgc gatctcgcgc tggcgagccg ccatgatccc cttgagcgac gggtagcgcg
      781 gcgcgcccag cgcctgggtg caggagatca ccgccggcat cggggcttcg atcaggtcgt
      841 agcccttcgc gctgagccgt ttgacccgca cgcggcccgc ggccggatcg ggctcgatgt
      901 gtgcggccgc ggacagcagg ggcagcccga ggcgggccgc gatccccgcc gcgaccgcgc
      961 cgccggtgcc gtcggacgtg tcgaggcccg tgagcaggag gtcgaaggtc tccgcggccg
     1021 ccgctgcggc gagcacgcgc accgtggccg gcagatcggc gcccgccagc gccggatcgg
     1081 tcaccagcac gccgcgctgg gcgcccatcg caagcgcctt gcgcatcgtc tcggtggcgt
     1141 tcgccggggc catcgcgagg atcgccacct cgccgccgtg ggcttcgacg agctggaggg
     1201 cggcctccag cgcgtactcg tcgttgccgt tcacgaccgt gggcgaggcc gagcggtcca
     1261 gccggaagtc cggcccgctc agtcgctccg cgcccgccgc cggatccggc acgggcttgg
     1321 tcaggacgag gatctgcacg tggcgagacg ctcctggctc gatggctggc tgaaccggtg
     1381 gggtctcacg gacgctgcgc gagcagcgcc ctggcgacga tgagccgctg gatctgttgg
     1441 gtgccttcgt agagctgcgt gatcttggcg tcgcgcatca tgcgctcgac cgggtattcg
     1501 tcgacgtagc cgtatccccc gaagacctgc accgcatccg tcgtcacccg catggcggcg
     1561 tcaccggcga cgagtttaca gatggccgcc caccgggcgg cgtcgggcgc gccggcctcg
     1621 atcgcctggc acgccgtgta caggagctgg cgcgccgcct cggtggatgc gtccatgtcg
     1681 gcaagcatcg cctggaccat ctgcagctcg ccgatcggcg ccccgaactg ccgccgctcg
     1741 gtcgcgtgcc tcgtcgccgc ctcgagcgcg ccctgcgcga tgcccacggc ctgggccgcg
     1801 atcccgggcc gcgaccgctc gaaggtctgc atcgcgatcg cgaaccccgc gccctcgtcg
     1861 cccagccggt tgctgatcgg gacccgcagt ccgtcgaacg cgagctcggc cgtcggcgag
     1921 ccccggatgc ccatcttgtg ctcgaggcgc gtcaccagaa agccggggcg atcggtctcg
     1981 acgaggatcg ccgacaggcg ccggctctgg cgcgtcgcgt ccgggttggt gacggcgaac
     2041 accacgacgt agtcggcgac gctcgcatgg ctgatgaagc gcttgccacc gtcgatcacg
     2101 tagtcgtcgc cgtcgatcac ggcgcgggtg cggatcccgg acgggtccga gccggcgtcc
     2161 gcctcggtca gggcgaacgc gatcagcttc tcgcccgacg cgagaccggg cagccagcga
     2221 tcctgctgct cgggcgtgcc ggcaacctgg atcgggatcg cgccaagcga ctggacggcg
     2281 aggatcaggc cggacgtcgc gcacgcccgg ctgatctgct cgatcgccag gcacagcgtc
     2341 aggaggtccg cgccgaggcc gccgtggcgt tccgcgaacg ggatcgcaag gatgtcgtgg
     2401 ctcgcgagga gccggcggac gtcgtcgggc cactcggccg tgcgatcgat ctcggcggcg
     2461 cgcggcgcga tctgctcgtc ggccagctcg cgcaccgcgt cacgcagcag gacctggtcc
     2521 tctgtgaggc ggatcgagcc gtgggcagtg gcactggtcg tcaccggaag cccatcctag
     2581 cagccagcca ggggcggatc gtcaggggtc gatcgtcagg ggtgacaccg acccatcagg
     2641 ctcgtacgct ggcccgacca acgcgcgggc cgcatcgggc tgtcggggac caacgggacc
     2701 gcgcctggag ggtgtgtgga ccagtcatac ctgctgctgt tggggatcgc gatcctggtc
     2761 gcgatcggat cgggtgcccg catccggaac cgcgtgctga ccgagcgggc cgatgctgcg
     2821 cagacggagt cgccgttcgc cgtctcgacc gagggcgaca agatctgccc caagtgcggg
     2881 atgggcaacc tctggaccga gcgccgctgc agcgcctgcg ggggcgccct caagggctga
     2941 ccgtcagagc accggcagcc ggcgtccctc ggggatctcg cggccgccga acgggacgat
     3001 cgcggccgcc agcagctcgc tgatgtcctg cgccgtgacc tggtcctcgc tgcccgcgcc
     3061 gcggcccgcg tcgttgagcc cgtcgcgcaa catcaccatg cagaacgggc acgcggtcgc
     3121 gaccgtctcc gcgcccgtcg cgagggcctg ctgtgtgcgc gccgcgttga cccgcgtccc
     3181 gcgcgactcc tccatccaca tccggccgcc gccggcgccg cagcagaagg tctggcgccc
     3241 gctgttctcc atctcgcgca gctcgacccc cgggatcgcg cccagcacct cgcgcggcgg
     3301 cgcgatcacg ccgttgtagc gggcgaggta gcaggcgtcg tggtaggtca cggtccgccc
     3361 cggcaggccg tcggcgccga gctggagccg gccggacgag accagcgacg agaggtactc
     3421 gctgtggtgg cggatcgtga acgacccgcc cagctggccg tactcgttgc cgatggtgtt
     3481 gaagcagtgg ggacacgcgg tgacgatcgt ccgctccccc atccggtacc ggttcagcgt
     3541 gtcgacgttc tgcgacgcga ggatctggaa cacgtactcg ttgcccatgc gccgggccgg
     3601 gtcgccggtg caggactcct cctggcccag cacggcgaac gagacgccgg cctcgaacag
     3661 gcaggtcgcg acggcccgcg cgacgcgccg gttgcggtcg tcgaacgcgg ccgcgcagcc
     3721 gacccagtac aggacctcga ggtcgccgag ctcgcccgcg gcctggacgg cggcgaccgt
     3781 ccggacctcg aatggcaggc ccttcgccca gtcgagccgc gaggatggcg gctggcccca
     3841 cgggttggcg acgctctcca tgcccttgaa cgccgccgtg agctcctgcg ggaaccggct
     3901 ctcctcgagc acgaggttgc gccggatgcc gacgatcttg tcgacgtgct cgatggtgac
     3961 ggggcacgcc tcgacacagg cgccgcaggt caggcagtcc cagacggcgt cgaacgggat
     4021 cgcggtgtcg acgaggggcc gcgccagcgc gtcggcgggc aggcgatcgt cgagcccggg
     4081 gcggatcgcg cgcaccgatg gcgagttggg aatgagcggc agcccggcct cggcgtccac
     4141 cgcctcgtgg cggatgccca tgatgaacga cttggggttg agcggcttcc cggtgttcca
     4201 ggcggggcaa gcctcctggc agcggccgca ctcggtgcag gtgaacccgt cgagcaggtc
     4261 cttccacgac aggtcggcca cggtccggat gccgaacgtc gcgtcctcgg cctcgaggtc
     4321 catcgccggc agctcgccgc gcggccggag cttgcgcagc gcgatgttgg ggatcgcggt
     4381 agcgatgtgg aggtgcttgc tgaacggcag gtagcacagg aacgccgcga ccaggaccat
     4441 gtgcgcccac cacaggatcg cgaatacggc ctcgagggcc gcctgatcgc cacccgagag
     4501 cgccgtccca gcggcgttgg cgatgaatgc gcccgggatg accccgtacg cggcgacctg
     4561 gaacacctgg gccagcagct cggtcgcgac gacgccgccg atcatgccca ggatcagcag
     4621 ggcgtcccgg ttgaaggtga ggcgcttggg cttcgtgac
//
LOCUS       DBNHXO010000069         2135 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_612775, whole genome shotgun sequence.
ACCESSION   DBNHXO010000069 DBNHXO010000000
VERSION     DBNHXO010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_612775"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..>2135
                     /locus_tag="AC162A_01550"
     CDS             17..>2135
                     /locus_tag="AC162A_01550"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR5009548.1"
                     /translation="MLSRPETVVFLRERLFELGLKEYRPLGDPTRFLDALATLFSRAK
                     DEDVSPDAYLAHAGRLAEEAAVATAAIDAAGGEAAATDRDAAAALTEDARRQAELARS
                     YARFQQLLAASGSIDFGDQVSLALRLLRESPAAREAVQRRFKFVLVDEFQDTNRAQAE
                     LVALVAERHRSVTVVGDDDQSIYKFRGAAISNILEFRERYRQARVVVLRRNYRSRAPI
                     LDAAYRLVRHNDPDRLEARAGIVKRLDPERGGDDAAPVRHEAFASGAEEADWIAADIA
                     RRLREGAHPRDVAVLVRTNAAADPLLRSLNLEGIPWRFSGTSGLYTRPEVRLLLAFLR
                     AIADPSSSVDVYALAASDLYALGAQDLVAIVNTARRRSRSIWEVLEELERQPGILRLS
                     PETRAAASRLVADLHRYVELANERSAGEVLYAFLRGSGWLARLAAADSVAAEEALSNV
                     ARFFDIVRAQSALLADDRAVFVARHLNTLIEAGDDPPTADIDPDVDAVAVMTIHKAKG
                     LEFLVVFLPGLVSGRFPATARREPLGLPLELVHETLPEGDYQLQEERRLFYVGMTRAR
                     DELVLSHAADYGGQRARRVSPFVLEALDLPVAAGTPGAGARSTTPLERLAGLERTEAA
                     PVSRRPRGDEPLVLSFYAVDDYLTCPLKYKYAHLLRVPLAPHHSLIYGSALHAAVAEF
                     HRRHARGDVMTEEQLYASFEQAWT"
BASE COUNT          261 a          786 c          779 g          309 t
ORIGIN      
        1 gccgccggac gcccgcgtgc tctcgcgccc ggagaccgtc gtgttcctgc gggagcgcct
       61 gttcgagctt ggactcaagg agtatcggcc gctcggcgac cccacgcggt tcctggacgc
      121 cctcgcaacc ctgttctcac gggccaagga cgaggacgtg tcgcccgacg cctacctcgc
      181 gcacgccggg cggctggcgg aggaggcggc cgtggcgacg gcggccatcg acgcggcggg
      241 gggcgaggcg gcggcgacgg atcgtgacgc ggcggccgcc ctgaccgagg acgcgcggcg
      301 gcaggcggag ctggcgcggt cgtatgcccg cttccagcag ctgctggcgg cgagcggttc
      361 gatcgacttc ggcgaccagg tctcgctcgc gctgcgcctg ctgcgcgagt cgccggccgc
      421 tcgcgaggcg gtccagcggc ggttcaagtt cgtgctcgtg gacgagttcc aggacacgaa
      481 ccgcgcccag gccgagctgg tcgctctggt cgccgagcgg caccgcagcg tgaccgttgt
      541 cggtgacgac gaccagtcga tctacaagtt ccgcggcgcc gcgatcagca acatcctcga
      601 gttccgcgag cggtatcgcc aggcgcgggt cgtcgtcctg cgtcgcaact accggtcacg
      661 ggcgccgatc ctcgacgcgg cctaccgcct cgtgcgccac aacgatccgg accggctcga
      721 ggcgcgggcc gggatcgtga agcgcctcga ccccgagcgg ggtggcgacg acgccgcgcc
      781 cgtccgccac gaggcgttcg cgagcggcgc ggaggaggcg gactggatcg ccgcggacat
      841 cgcccgccgc ctccgcgagg gtgcccatcc gcgggacgtc gccgtcctcg tccgtacgaa
      901 cgcggcggcc gacccgctgc tgcgctcgct caacctcgaa gggatcccgt ggcgcttctc
      961 gggcaccagc gggctataca cgcggcccga ggtgcgcctg ctgctggcct tcctgcgcgc
     1021 catcgccgat ccgtcgtcgt cggtcgacgt ctacgcgctc gccgcctcgg acctgtacgc
     1081 gctgggggcg caggacctcg tcgccatcgt gaacaccgcc cgccggcgga gccgctcgat
     1141 ctgggaggtg ctggaggagc tcgagcgcca gcccgggatc ctgcgcctct ccccggagac
     1201 ccgcgccgct gcgtcgcggc tggtcgcgga cctgcaccgg tacgtcgagc tggccaacga
     1261 gcggtcggcc ggcgaggtcc tgtacgcgtt cctgcgggga tcgggctggc tcgcgcggct
     1321 cgccgccgcc gacagcgtcg cggccgagga ggcgctctcg aacgtcgccc gcttcttcga
     1381 catcgtgcgg gcccagtcgg cgctgctggc cgacgaccgg gcggtgttcg tcgcgcgtca
     1441 cctcaacacg ctgatcgagg cgggtgacga cccgcccacc gcggacatcg accccgacgt
     1501 cgacgcggtc gcggtgatga cgatccacaa ggcgaagggc ctcgagttcc tcgtcgtctt
     1561 cctgcccggg ctcgtcagcg gacggttccc cgccaccgct cgccgcgagc ccctgggcct
     1621 gccgctcgag ctggtgcacg agaccctgcc cgagggcgac taccagctcc aggaggagcg
     1681 tcgtctgttc tacgtgggga tgacgcgggc gcgcgacgag ctcgtgctgt cgcatgccgc
     1741 ggactacggc ggccagcggg cccggcgcgt gtcgccgttc gtgctggagg cgctcgacct
     1801 gccggtcgcg gccggtacgc cgggtgcggg cgcgcggtcg acgacgccgc tggagcgcct
     1861 ggccgggctg gagcggacgg aggcggcccc cgtgtcgcgt cgcccgcggg gcgacgagcc
     1921 cctcgttctc tcgttctacg ccgtggacga ctacctgacc tgtccgctca agtacaagta
     1981 cgcccacctg ctgcgcgtgc cgctcgcgcc ccatcactcg cttatctatg gctcggcact
     2041 ccatgcggcg gtcgccgagt tccatcgccg gcacgcgcgc ggggacgtga tgaccgagga
     2101 gcagctgtac gcgtcgttcg agcaggcgtg gacca
//
LOCUS       DBNHXO010000070         3175 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2398909, whole genome shotgun sequence.
ACCESSION   DBNHXO010000070 DBNHXO010000000
VERSION     DBNHXO010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2398909"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="AC162A_01555"
     CDS             complement(<1..682)
                     /locus_tag="AC162A_01555"
                     /inference="COORDINATES: protein motif:HMM:NF017984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase AlkZ-like family protein"
                     /protein_id="HIR5009549.1"
                     /translation="MTPDERTTAFSFRRQRLDGSAGSAVEAIGSVVGVYSANPTGPLS
                     IHVRAPSASPESILALDSSRATMRMRAMRTSAFVVPRASAALVAAATAVPLERFAWML
                     RAAHIAPEELPAVRDAVLAAAAQPGTAQQLRGRVAIDGLDVGSLLSWLSLRGDVVSIG
                     SGSLTSNASRYVARDAWLAGDPEPAAPEPAAARAWLAGEYLRAFGPARVADFAWWSGL
                     NGAAAASAI"
     gene            885..>3175
                     /locus_tag="AC162A_01560"
     CDS             885..>3175
                     /locus_tag="AC162A_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5009550.1"
                     /translation="MALALDDSTPLRRRRATRRQQAAPDVGTHEARAVALRRIVAEVN
                     GTLELDAVLEDVLDSSQELFGADAAGLWQLQPRRQPFELVASRDLGQELIDEVAAVHE
                     DDDVMGLRAMRERRPIVIADPAAAPRFGPVYERLGFRTVNFVPLLFRDEHVGLLVLYH
                     RTPYDWSPDELELCASFAHQMATAFANARLFARVRAGNARLRAIQELSSRLNRIQQVE
                     KIGDAIVAEADRLINHDTIRVYTVDEAAGMCEPIAYHGEVSGIGALNREVLRLAIGEG
                     LTGWVAAHNETIRLGDAGSDPRGLQLGTDDVPESMLLVPMSYESRVLGVIVVSKVGYD
                     QFGEDDQRTLEIFAGYAAQAVVNAEAFGQVRRQQEELSHRLDSQRRLLEVNERLLATL
                     DPSGVLEMIADSLKAVVSYDALTVYRVDRVAHVRRAVLARDRYAEVILQHEGPLDAGI
                     TGWVINSGEALLANDVHLDERSVQIPGTPEEPESMLVCPLFGAGDVIGTLNVARMGEA
                     EAHFSQDEFELVQLFAAQASIALRNAEAHGAVMTQAEHDALTGLRNHGAFQREIGLLI
                     ARGEPFGLLMLDLDAFKAYNDWHGHPEGDALLARIATAMAGALRDGDHVYRYGGDEFA
                     VLLPRISAAASRDVTERIRSAVARLTGDVGPSVTVSAGAAFYPEDAREKDDLVAVADR
                     ALYLAKPADRLRLGIDDPTRDPYLAAIDQMTLRLLERLEPRQLLREIVDRAASLVGVK
                     HGFLYLLEDNDGTTDLVCRVGVGL"
BASE COUNT          467 a         1183 c         1122 g          403 t
ORIGIN      
        1 cgatcgcgga tgccgccgcg gcgccgttca gccccgacca ccacgcgaag tccgccactc
       61 gcgccggtcc gaacgcccgc aggtactcgc ccgcgagcca ggcgcgggct gccgcgggct
      121 cgggcgccgc gggctccggg tcaccagcga gccacgcgtc gcgcgcaacg tagcgcgagg
      181 cgttcgacgt cagcgagccc gacccgatcg agacgacgtc gccccgcagc gagagccacg
      241 acagcagcga gccgacgtcg aggccgtcga tggccacccg accccgcagc tgctgtgccg
      301 tgcccggctg ggccgcggcc gccaggacgg cgtcgcggac cgcgggcagt tcctcgggcg
      361 cgatatgcgc cgcgcgcagc atccaggcga accgctcgag gggaacggct gtcgcggctg
      421 cgacgagcgc ggccgatgcc ctgggcacga cgaacgccga ggtccgcatc gcccgcatcc
      481 gcatcgtcgc ccgcgacgag tccagcgcca ggatcgactc gggcgacgcg gagggggccc
      541 ggacgtggat cgacaggggt ccggtcgggt tcgcgctgta gacgccgacg accgacccga
      601 tcgcctccac cgccgagccc gccgagccgt ccaggcgctg ccggcggaac gagaaggcgg
      661 tcgtgcgctc gtcaggggtc acgaggtgag tatcgacgcg gcagccgcac gtcaaccgcg
      721 ccgaacggca accccccgtt cgtgcagtcc ggctgcacgc tggtacgacc gtcctatcgt
      781 gcgcagccgc cacgctcgcg tacgttgcgt tccgacacag cccgaatcac cccctgtgcc
      841 acggggaggg cgcagggccg cagggggtaa ccacgacccg tcgcatggca ctggccctcg
      901 acgacagcac accgctccgg cgacgccgcg ccacgcgccg gcaacaggcc gcccccgacg
      961 tcggcacgca cgaggcgcga gccgtcgccc tgcgccgcat cgtggccgag gtcaacggca
     1021 cgctcgagct cgacgccgtg ctcgaggacg tcctcgactc ctcgcaggag ctgttcggcg
     1081 cggacgccgc ggggctttgg cagctgcagc cgcgccgcca gccgttcgag ctggtcgcga
     1141 gccgcgacct gggccaggag ctgatcgacg aggtcgccgc cgtccacgag gacgacgacg
     1201 tgatgggcct gcgcgcgatg cgcgagcgac gcccgatcgt catcgcggac ccggcggcag
     1261 cgccgcgatt cggcccggtc tacgagcggc tcggcttccg caccgtcaac ttcgtcccgc
     1321 tcctgttccg cgacgagcac gtcggcttgc tggtcctgta ccaccggaca ccttacgact
     1381 ggtcgcccga cgagctcgag ctgtgcgcct cgttcgcgca ccagatggcc accgcattcg
     1441 cgaacgcccg cctgttcgcg agggtccgcg ccggcaacgc ccggctgcgc gcgatccagg
     1501 agctgtcgtc gcgcctcaac cggatccagc aggtcgagaa gatcggcgac gcgatcgtcg
     1561 cggaggcgga ccgcctgatc aaccacgaca cgatccgcgt gtacaccgtc gacgaggccg
     1621 ccgggatgtg cgagccgatc gcctaccacg gcgaggtctc cgggatcggc gcactcaatc
     1681 gcgaggtcct gcggctcgcg atcggtgaag gactgacggg ctgggtcgcc gcccacaacg
     1741 agaccatccg cctgggcgac gccgggtcgg atccccgcgg cctccagctc ggcacggacg
     1801 acgtcccgga gtcgatgctg ctcgtcccga tgtcgtacga gtcgcgcgtc ctgggcgtga
     1861 tcgtcgtgtc caaggtgggc tacgaccagt tcggcgagga cgaccagcgg acgctcgaga
     1921 tcttcgccgg gtacgcggcc caggcggtgg tcaacgccga ggcattcggg caggtgcgcc
     1981 gccagcagga ggagctgagc caccggctgg atagccagcg ccgcctgctc gaggtcaacg
     2041 agcgcctgct ggcgaccctg gatccgagcg gcgtgctcga gatgatcgcc gactcgctca
     2101 aggccgtcgt ctcgtacgac gcgctgacgg tctaccgggt cgaccgggtc gcccacgtgc
     2161 ggcgagccgt gctcgcccgc gaccgctacg cggaggtgat cctccagcac gagggcccgc
     2221 tcgacgccgg catcaccggc tgggtcatca acagcggcga ggcgctgctc gccaacgacg
     2281 tgcatctcga cgagcggtcg gtccagatcc ccggcacccc cgaggagccc gagtcgatgc
     2341 tggtctgccc gttgttcggg gcgggcgacg tcatcggcac gctcaacgtg gcgcgcatgg
     2401 gcgaggcgga ggcgcacttc agccaggacg agttcgagct ggtgcagctg ttcgcggccc
     2461 aggcgtccat cgcgctgcgc aacgccgagg cgcacggcgc cgtgatgacc caggcggaac
     2521 acgacgcgct caccgggctg cgcaaccacg gcgcgttcca gcgcgagatc gggctgctga
     2581 tcgcgcgcgg cgagccgttc ggcctgctga tgctcgacct cgacgcgttc aaggcctaca
     2641 acgactggca cgggcatccc gagggcgacg cgctgctggc ccgcatcgcg accgcgatgg
     2701 ccggggcgct gcgcgatggc gaccatgtct accgctacgg cggcgacgag ttcgcggtgc
     2761 tcctgccgcg catctcggcc gcggcgtccc gcgacgtgac ggagcggatc cgatcggcgg
     2821 tcgcgcggct caccggtgac gtcggcccgt ccgtgacagt cagcgcgggc gccgcgttct
     2881 acccggagga cgcacgcgag aaggacgacc tcgtggccgt cgcggaccgg gccctctacc
     2941 tcgcgaagcc cgccgaccgc ctccgcctcg gcatcgacga cccgacgcgc gacccgtacc
     3001 tcgccgccat cgaccagatg acgctgcgcc tgctcgagcg gctggagccg cgccagctgc
     3061 tgcgcgagat cgtcgaccgc gccgcgagcc tcgtcggcgt gaagcacggc ttcctgtacc
     3121 tgctcgagga caacgacggc accacggacc tcgtgtgccg ggtcggcgtc gggct
//
LOCUS       DBNHXO010000071         1722 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_216938, whole genome shotgun sequence.
ACCESSION   DBNHXO010000071 DBNHXO010000000
VERSION     DBNHXO010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_216938"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="AC162A_01565"
     CDS             complement(<1..1222)
                     /locus_tag="AC162A_01565"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: protein motif:HMM:NF012471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR5009551.1"
                     /translation="MHRLLRRLVLLPSIAIALVVPAIAPATALAANEFPDGWKGYHSY
                     PEMVDDIKAVAAAHPSIVRLFSIGDSYQGRKLWAAKISDNVGTDENEPEVLYDGLHHA
                     DEHMSLEMTLHILHWLADDYGSKARITDIVNSREVWIVFAMNPDGAQFDIRDGRFHYW
                     RKNRQPTPGSSAVGTDLNRNYGYRWGVAGRTSNNPEAITYHGPNAFSAPETRAMRDFL
                     ASRVVNGRQQIRAAITFHESGRLVMWPYGYTHKNVPKDMTPDDHAALRKIGRHMASTN
                     GYKPEQGSDLYITRGGSRDYLYGMYRIFAYTFELSVKDYPKPRMIDPETGRTREAVLW
                     LAEKAWCPLTVLGDAVRTARCGALDDDLEVGRGWQVNPGGTDTATTTAAGRWARGNPP
                     GTKTGGVTLQPTTVP"
     gene            1365..>1722
                     /locus_tag="AC162A_01570"
     CDS             1365..>1722
                     /locus_tag="AC162A_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5009552.1"
                     /translation="MTAERIKITYATLRADNEDLQVAYEAGLERAKGRLGAYHRNFID
                     GEERDGEGSFELRSPIDSDLLVGTFARGTRADVQDAIRAARRAQPAWFRLGWEKRLEI
                     MKRAADLISERQMEYAG"
BASE COUNT          251 a          567 c          622 g          282 t
ORIGIN      
        1 acggcaccgt cgtcggctgg agtgtcacgc cgccagtctt cgtgcccggc gggttgccac
       61 gcgcccagcg gccagcggcc gtcgtcgtcg cggtgtcggt cccgcccggg ttaacctgcc
      121 agccgcggcc gacctcgagg tcgtcgtcga gcgccccgca gcgcgcggtc ctgaccgcgt
      181 cgccgagcac ggtcaacgga caccacgcct tctccgccag ccacaggacg gcctcgcggg
      241 tgcgaccggt ctcggggtcg atcatcctcg gcttcgggta gtccttgacc gacagctcga
      301 acgtgtacgc gaagatccgg tacatgccgt acaggtagtc acgcgacccc ccgcgggtga
      361 tgtacaggtc gctgccctgc tcgggcttgt agccgttggt cgaggccatg tgccggccga
      421 tcttgcgcag ggcggcgtga tcgtcggggg tcatgtcctt cgggacgttc ttgtgcgtgt
      481 agccgtacgg ccacatcacc agccggccgg actcatggaa cgtgatcgcg gccctgatct
      541 gctgacgtcc gttgacgacc cgcgacgcca ggaagtcgcg catcgcccgg gtctcggggg
      601 cggagaaggc gttcggcccg tggtaggtga tcgcctcggg attgttgctg gtccggccgg
      661 caacacccca tcggtagccg tagttgcggt tgaggtccgt gccgacggcc gacgagcccg
      721 gggtcggctg gcggttcttg cgccagtagt ggaagcggcc gtcccggatg tcgaactgcg
      781 cgccgtccgg gttcatcgcg aacacgatcc agacctcgcg gctgttcacg atgtcggtga
      841 tccgcgcttt cgacccgtag tcgtcggcga gccagtggag gatgtgcagg gtcatctcga
      901 ggctcatgtg ctcgtcggcg tggtggaggc cgtcgtacag gacttccggc tcgttctcgt
      961 cggttccgac gttgtcactg atcttggcgg cccagagctt gcggccctgg tagctgtcgc
     1021 cgatcgagaa caggcgcacg atcgacgggt gggcggccgc gaccgccttg atgtcgtcga
     1081 ccatctcggg gtagctgtgg tagcccttcc agccgtccgg gaactcgttc gcggccaggg
     1141 ccgtggcggg ggcgatggcc ggcacgacga gggcgatcgc gatcgacggg aggagcacga
     1201 ggcggcgcag gagacgatgc atgggaccag catgcggggt cgtgtcgagt ccgggacgcc
     1261 ggacgatggt cccggtcgcg gcacccagac tagactggcg gcaacatcga cctcgcgcgc
     1321 cctccgccat caatgcggac ggtcgccctc acgggagccc tgcgatgacc gctgagcgga
     1381 tcaagatcac ctacgcgacc ctgcgcgccg acaacgagga cctccaggtc gcctacgagg
     1441 ccggcctgga gcgggcgaag gggcggctcg gcgcctatca ccgcaacttc atcgacggcg
     1501 aggagcgcga cggcgagggc tcgttcgagc tgcggtcacc catcgacagc gacctcctgg
     1561 tcggaacgtt cgcccgcggc acacgtgctg acgtccagga cgcgatcagg gccgcccgca
     1621 gggcgcagcc ggcctggttc cggctcgggt gggagaagcg actcgagatc atgaagcgcg
     1681 cggcggacct gatctccgag cggcagatgg agtacgcggg cc
//
LOCUS       DBNHXO010000072         2814 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3192655, whole genome shotgun sequence.
ACCESSION   DBNHXO010000072 DBNHXO010000000
VERSION     DBNHXO010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3192655"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /gene="secD"
                     /locus_tag="AC162A_01575"
     CDS             <1..1439
                     /gene="secD"
                     /locus_tag="AC162A_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR5009553.1"
                     /translation="GGLYHPAPMRRAGPILIIVIGVLAILVDFVRVPIPSMGGQDAGT
                     THFLETKLGLDLQGGLRIEYQVLPAEGKTPTREDLAILRQIIINRVDKSGVAEPQVVV
                     QGNDRIVVEMPGIQNADQIRNLVGTTGRLDFVPLGQLTQGPPQGTELPAATIGTNCQE
                     FADASKPIENCVLFSGDQVAAASIGANQAGQRTVDFTLNSAGKDLFAKYTQEHTGDIF
                     AIVLDGKVISAPQINEPIPGGQVQISQNSSIGGYPLAEAQNLVTILQFGQLPFPIAEL
                     ANTTVSPTLGEAFLHQSLIAGLIAILMVIFFMVVHYRLPGVVASGALIYYALVVYALF
                     RLIPVTMTLAGVAGFVLSVGMAVDANILIFERTKEELRVGKSLPAAIEAGFNRAWNSI
                     LDSNVSSLITATILFVLGSSVIRGFGLVLIVGVLVSMFTAITVTRTVLRIVVAQPWAR
                     KASLYGVTEEEFLARPTSTRSREARARV"
     gene            1432..2616
                     /gene="secF"
                     /locus_tag="AC162A_01580"
     CDS             1432..2616
                     /gene="secF"
                     /locus_tag="AC162A_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR5009554.1"
                     /translation="MFDVVGKRRWFFAFSLAITIPGLIFLVMGFVNPKVGLQFAIDFT
                     GGTVWTIRFEDPNVSPQQVKDVLEGQGIEANVTETGTGFMEIRTKEAALAAPPAPAPT
                     LSPAPSGSPAVSPSPGASSSAVPSASAGASPSASAAASASASPSPSSSAAASPGASPS
                     ASPGAIIGPGGALPTQGRLGEIRFALEDALGTIAEQQSLSTVGAVVSSDLILQALLLI
                     LFGSIGILIWITIRFNDFRMGATALVAMLHDVLVVVGAFAILGTFAGLQIDSLFVTAM
                     LTVIGFSVHDTIVVFDRVRENKARHVGESFDRIVNHSILQTLGRSITTSLTVVITLLT
                     LLLFAGDAIGTFVLALLLGIVSGTYSSIFNAAPLLVVWHNRDDKRRQSELAARPSRRP
                     AT"
BASE COUNT          413 a         1020 c          914 g          467 t
ORIGIN      
        1 gcggcggcct gtatcatccc gccccgatgc gacgcgccgg cccgatcctg atcatcgtta
       61 tcggtgtgct cgcgatcctg gtggacttcg tcagagtccc catcccgtcg atgggcggcc
      121 aggacgcggg cacgacccac tttctcgaga cgaagctcgg ccttgacctg cagggcggcc
      181 tgcggatcga gtatcaggtg ctgccggccg agggcaagac gcccacgcgc gaggatctcg
      241 ccatcctgcg ccagatcatc atcaaccgcg tcgacaagtc gggcgtcgcc gagccccagg
      301 tggtcgtcca gggcaacgac cggatcgtcg tcgagatgcc gggcatccag aacgccgacc
      361 agatccgcaa cctcgtcggg accaccggcc gcctcgactt cgtgcccctc ggacagctga
      421 cgcagggacc cccgcagggg acggagctgc ccgcggcgac gatcgggacc aactgccagg
      481 agtttgccga cgccagcaag ccgatcgaga actgcgtgct gttctcgggc gaccaggtcg
      541 ccgccgcgtc gatcggcgcc aaccaggcgg gtcagcgaac ggtcgacttc acgctcaaca
      601 gcgccggcaa ggacctgttc gcgaagtaca cccaggagca caccggcgac atctttgcga
      661 tcgtcctcga cgggaaggtc atcagcgccc cccagatcaa cgagccgatc ccgggcggcc
      721 aggtccagat cagccagaac agcagcatcg gcggctaccc gctggccgag gcgcagaacc
      781 tggtcacgat cctccagttc ggccagctgc cgttcccgat cgcggagctg gccaacacga
      841 ccgtcagccc gacgctcggt gaggcgttcc tgcaccagag cctgatcgcc ggcctgatcg
      901 ccatcctgat ggtgatcttc ttcatggtcg tgcactaccg gctgcccggc gtcgtcgcca
      961 gcggcgcgct catctactac gcgctggtcg tctacgccct gttccgcctg atcccggtca
     1021 cgatgaccct cgccggcgtg gcgggcttcg tgctctcggt cggcatggcg gtcgacgcca
     1081 acatcctgat cttcgagcgc accaaggagg agctgcgggt cggcaagagc ctgcctgcgg
     1141 cgatcgaggc gggcttcaac cgggcctgga actcgatcct cgactccaac gtgtcgagcc
     1201 tgatcacggc gacgatcctg ttcgtgctgg gctcgtcggt gatccgcggc ttcggcctcg
     1261 tcctgatcgt cggcgtgctg gtctcgatgt tcacggcgat caccgtcacc cgcacggtcc
     1321 tgcggatcgt ggtcgcgcag ccgtgggcgc gcaaggcgag cctgtatggc gtgaccgagg
     1381 aggagttcct ggcccgcccg acgagcaccc gcagccgcga ggcgcgcgcg cgtgtttgac
     1441 gtcgtcggga agcggcgctg gttcttcgcg ttctcgctcg ccatcaccat cccgggcctg
     1501 atcttcctcg tcatggggtt cgtgaacccc aaggtcggcc tgcagttcgc gatcgacttc
     1561 accggcggca cggtgtggac catccggttc gaggacccca acgtcagccc gcagcaggtc
     1621 aaggacgtcc tcgagggcca ggggatcgag gccaacgtca ccgagaccgg cacgggcttc
     1681 atggagatcc gaaccaagga agccgcgctc gccgcgccgc ccgcgccggc gccgaccctc
     1741 tcgccggcac cgtcgggctc gcccgcggtc tcgccgtcgc cgggcgcgtc gtcgtccgcg
     1801 gtcccatcag cgtccgcggg cgcgtcaccc tcggcctcgg ccgcagcatc cgcctcggcg
     1861 agcccgtcgc cgtcgagctc ggcggccgcc tccccagggg cgagtccgtc cgcgagcccg
     1921 ggcgcgatca tcggacccgg cggcgccctg ccgacccagg gcaggctcgg tgagatccgg
     1981 ttcgccctcg aggatgccct gggcacgatc gccgagcagc agtccctctc gacggtcggc
     2041 gctgtggtat cgagcgacct gatcctccag gccttgctcc tgatcctgtt cggctcgatc
     2101 gggatcctga tctggatcac gatccgcttc aacgacttcc ggatgggcgc gaccgccctg
     2161 gtcgcgatgc tccacgatgt gctcgtggtg gtcggggcgt tcgcgatcct cggcacgttc
     2221 gccggcctgc agatcgacag cctgttcgtg accgcgatgc tgaccgtgat cgggttctcg
     2281 gtccacgaca cgatcgtggt gttcgaccgg gtccgcgaga acaaggcgcg gcacgtcggc
     2341 gagtcgttcg atcgcatcgt caatcactcg atcctccaga cgctgggtcg atcgatcacc
     2401 accagcctga ccgtggtgat caccctgctg acgctgctgc tgttcgcggg cgacgcgatc
     2461 gggacattcg tcctcgcgct gctgctcggg atcgtgtccg ggacctattc ctcgatcttc
     2521 aacgccgccc cgctgctcgt cgtgtggcac aaccgcgacg acaagcggcg ccaatccgag
     2581 ctcgcggctc gcccaagccg ccgacccgcc acgtgacgac gggacccggc cgaggctggg
     2641 acccgattct cacctctggg ttcctgatgg acccgaggtc cggcaccgcc tcgccgtccc
     2701 gtcgagtccg gggtaagccg gtcgtggcat cctccagacg atgatcgacg cgcgtcaccg
     2761 ctgggtggtc ccggagccgc tgcacctcga cccggcgttg cgctcggcgg cccg
//
LOCUS       DBNHXO010000073         1536 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2002424, whole genome shotgun sequence.
ACCESSION   DBNHXO010000073 DBNHXO010000000
VERSION     DBNHXO010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2002424"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="AC162A_01585"
     CDS             complement(<1..745)
                     /locus_tag="AC162A_01585"
                     /inference="COORDINATES: protein motif:HMM:NF014615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR5009555.1"
                     /translation="MSLPRDRFATRLAAAPLLADGGMGTLLFSRGIPQRACLDELATT
                     RSDVVAALHREYLDAGADLIETLTFGANRIRLEAFGLGAQAGRFNRRAAQVAREARDV
                     SGRDAWVGGSIGPLGPPTRELSHPDEATIRAAFREQVDGLLEGGADLLVIETFFDLEH
                     LLLAADEARRAADVPVIASMTFGEDLVLADGTTPEVAVRALTAAGVDAIGVNCGVGPL
                     ACLDALARFGPSADGDPARSIMPNAGLPQR"
     gene            complement(755..>1536)
                     /locus_tag="AC162A_01590"
     CDS             complement(755..>1536)
                     /locus_tag="AC162A_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III PLP-dependent enzyme domain-containing
                     protein"
                     /protein_id="HIR5009556.1"
                     /translation="TETELTAAAAGLAPDGPLSARGIHLHVGSQLGAVDAWRDAVRRG
                     LAVLALIGAGRPGFDTFDVGGGFPVGEPGTVPTPAHFGREMPALLDALPLDRRPARLA
                     VEPGRFLVASASWLVARVLHVRERADADRVIVLDTGMTELIRPALYGASHPIVALTSL
                     GRPLDAEGRKPSGALAPIALARVDGPICESTDTFDRHDLPPLRRGDLVAIREAGAYAA
                     SMAMTYNGRPAPTQAMLETNGALTLGRRRRAVAIRRGATTG"
BASE COUNT          198 a          551 c          611 g          176 t
ORIGIN      
        1 ggcgctgcgg caggccggca ttgggcatga tcgagcgggc ggggtcgccg tccgccgacg
       61 gcccgaagcg ggcgagcgcg tcgaggcagg cgagcggccc gacgccgcag ttcaccccga
      121 tcgcgtccac gccggcggcg gtgagggcgc ggacggcgac ctcgggcgtc gtcccgtcgg
      181 ccagcaccag gtcctcgccg aaggtcatcg acgcgatcac cgggacatcg gcggcgcgcc
      241 gcgcctcgtc ggcggcgagc aggaggtgtt cgaggtcgaa gaaggtctcg atgacgagga
      301 ggtcggcgcc cccttcgagc agcccgtcga cctgctcccg gaacgcggcc cggatggtgg
      361 cctcgtcggg gtggctgagc tcgcgggtgg ggggcccgag gggcccgatg ctgccgccga
      421 cccacgcgtc acggccgctg acgtcgcgcg cctcgcgggc gacctgcgcc gcgcgacggt
      481 tgaacctgcc ggcctgcgcg ccgaggccga acgcctcgag ccggatccgg ttggcgccga
      541 aggtgagggt ctcgatgagg tccgcacccg cgtcgaggta ctcacggtgc agcgcggcga
      601 ccacgtccga gcgggtcgtc gcgagctcgt ccaggcacgc tcgctgcggg atcccgcgcg
      661 agaacagcaa cgtacccatg ccgccgtcgg cgaggagcgg cgcggcggcg agacgggtgg
      721 cgaatcggtc gcggggcagg ctcatgacgc aagcctatcc ggtcgtggcg ccccggcgga
      781 tcgcgacggc ccggcgccgg cggccgagcg tgagagcccc gttcgtctcg agcatggcct
      841 gggtgggtgc tggccggccg ttgtacgtca tcgccatgga cgccgcatac gccccggcct
      901 cgcggatcgc gacgaggtcc ccgcgccgca gtgggggcag gtcgtggcgg tcgaacgtgt
      961 cggtgctctc gcagatcggg ccgtcgacgc gggcgagggc gatcggtgcg agggcacccg
     1021 agggcttgcg gccctcggcg tccagcggcc ggccgagcga ggtcagggcg acgatggggt
     1081 ggctggcgcc gtagagcgcg gggcggatga gctcggtcat gccggtgtcg aggacgatga
     1141 cccggtcggc gtccgcgcgc tcgcgaacgt gcagcacgcg cgccacgagc cagctggcgc
     1201 tcgcgaccag gaaccggccg ggctcgaccg cgagccgtgc cggtcgccga tccagcggca
     1261 gggcatcgag cagcgccggc atctcgcgtc cgaagtgcgc cggcgtcggg acggtaccgg
     1321 gctcgcccac cgggaagcca ccgccgacgt cgaacgtgtc gaaccccggc cggccggcgc
     1381 cgatcagcgc gagcaccgca agcccgcgcc ggacggcgtc gcgccacgcg tcgaccgcac
     1441 ccagctgcga gccgacgtgg aggtggatgc cccgcgcgct caacggcccg tccggcgcga
     1501 gccctgccgc cgccgccgtc agctcggtct ccgtca
//
LOCUS       DBNHXO010000074         6859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3192935, whole genome shotgun sequence.
ACCESSION   DBNHXO010000074 DBNHXO010000000
VERSION     DBNHXO010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3192935"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="AC162A_01595"
     CDS             complement(<1..903)
                     /locus_tag="AC162A_01595"
                     /inference="COORDINATES: protein motif:HMM:NF022702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 N-terminal domain-containing protein"
                     /protein_id="HIR5009557.1"
                     /translation="MSRSATGAIVVVVLVVGALVGIGAASMLTAAPGATSTPIAGVTA
                     PPPDPTTPSSPGGSPVPTEPGGSSAEPPPSTEPSASPSPTPVPTPVTAPAPLTGMPVK
                     PAQAERRVVAVMIDDQFDARPQSGLSDADVVWQAPAEGGIPRYMAFFQTGDPPAVGPV
                     RSSRLYFIAWASEWRSLYVHAGGAPQAKALLASSKGRGSYVYNADEFRWGGGRYLWRI
                     NTRFAPHNVYTDGKHLRRLAKRVGADPVPGQKPVWKFGDPVPLEERPEGGTISVPYLA
                     NKITYKYNRATNRYQRSVSGEGKQV"
     gene            1143..1847
                     /locus_tag="AC162A_01600"
     CDS             1143..1847
                     /locus_tag="AC162A_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009558.1"
                     /translation="MPDDTPTPPKRTRKRSTRKPSPAELVGVMAPDPEPDPGLVAVEE
                     AGEADAAAPAADEAATVADAAGSAAAPEEPTGGGAPAASPGLPDTVLRIERGGIGEAT
                     AGSVEVHVGGIGALDAEDVFVQWGGVGAARADHLAVEFGSVGAALAGEMRLTQGFASA
                     VAAREATIEQALVRTLIAQEVTINRPTGVLVMIAQRVSGDVRPVLDWRGALAAGAAFA
                     LITALIRGLREARDRS"
     gene            complement(1877..2710)
                     /locus_tag="AC162A_01605"
     CDS             complement(1877..2710)
                     /locus_tag="AC162A_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5009559.1"
                     /translation="MTVPASDRVVIRAERLGVRYSLHLTRKQTIRDSIVHRVRRVGDP
                     DFWALRDASFTVLQGESVGVIGPNGAGKSTLLQVLAGILVPSEGVVEVDGHISSLLTL
                     GVGFDEDLSGVDNIRLAGAFMGIDHRVVEERLPGIIEYAEIGQFIDAPIKTYSAGMRA
                     RLGFSIATSVEPDILLLDEVLATGDAAFREKSKARVLELVREAKAIVLVTHDMNWVTE
                     YCNRAILIEKGRIAHFGSPDEVVELHKEHMAERRAEAKEKGVLRLRTRTPGSGGRRRK
                     G"
     gene            complement(2730..3602)
                     /locus_tag="AC162A_01610"
     CDS             complement(2730..3602)
                     /locus_tag="AC162A_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009560.1"
                     /translation="MTATSEAPASFRSSSVRPGALSLVQGGIREVLSRRRLIAYLVRA
                     DLRKSGSDTLLGNLWWFMDPLLQMLVYVIFVGIILQRGGAIEDYPLFVLCAILPWKWF
                     QSAVGDGVSSIVSRERLIKQIFFPKLVLPVSTTMAGVVSFAFGLVPLLAITLIFYRGH
                     LSAWFLLIPLVAVVQLVFSLGVVILVSAANVFYRDVGNLTRHLLRMWFYLSPALYPAE
                     LVKEIAKDNPIIADLFALNPWTVLFGAYRDLIFYGRAPHWTGLAILLAVSVVMVLIAV
                     YLFKRAEPSFAKVL"
     gene            3804..5486
                     /locus_tag="AC162A_01615"
     CDS             3804..5486
                     /locus_tag="AC162A_01615"
                     /inference="COORDINATES: protein motif:HMM:NF025002.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR5009561.1"
                     /translation="MLLNVGFGLTIAIALLLLLVAVGYSWYNDHLSPVGSVNGQSISK
                     DAFRKQVAINDFRADYQNRRIRTLLTAGHLRTADAQARQAVLDQRNQQKATISLEQLI
                     DGAVMAQLATSKNVTVADADVDARLAEDATTPELRHAWMIAVAPELAEGETKATDAEK
                     AAAKAKADQALADLRAGKDWETVAKAVSTDGTKNQGGDIGYIDENAALDPPFVEAVMA
                     AALNATTDVIEGADGTYRIGRVTDIVAPVVDATYLDQVKDAGIDLSDYREALRRDVLR
                     TKLSDAVVAQYLAPGPQREVSEIHLGVDVDPSTGSPTGKESDPTAVKIRHILYSPAGD
                     AQAAASLAPDDAAWKAAEEKANAAYQKLKADPSLFASLVASDSDDGGSSSRGGSYWFT
                     PDDGLLPEFQEAIFKPGLTPGQLLEPVKTDAGWHVIQIQHFPTDLEWANTLKQQIDAG
                     TLTFADAARDNSDTWDAATAGDDQWILKGQLSPEVEAALFAAPIGKLSSPLAVEGDGV
                     YLFLVSQEQNRAPDAEQKASLESSAFPTWYSTQKGAYAIERDPEITASGASG"
     gene            5509..6675
                     /gene="mazG"
                     /locus_tag="AC162A_01620"
     CDS             5509..6675
                     /gene="mazG"
                     /locus_tag="AC162A_01620"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HIR5009562.1"
                     /translation="MLDALLAEARLRWGLDPAAGLCVVVTEQLVATPIEPSIAQLIVP
                     AARLRAGIPGANPAPLPGRHGHGGEPIETLRRLYPAGHPVGRFGQPDGTTLADLTEGD
                     LGSPVYLAPVAPVADLASPWGMPWISARLREPDGCPWDREQTHASLRNHLLEEAYEVY
                     DALDDGATPELAGELGDLLLQVVLHAQLAAEQGVFDMTDVWSAIASKIVHRHPHVFGD
                     AEARTASDVNRQWERIKAGERAEAASGSGDGADVAPTSALDGISRTLPALAASQEMQE
                     RAANLGYDWPSIDGVLDKVREEVGELVEAEDAAHRTEELGDLLFVLVNVGRKTGIEVE
                     GALRGANEKFRGRFRHVELAAAARNVALRDMSFEELDALWDAAKAAERGEARTS"
     gene            6672..>6859
                     /locus_tag="AC162A_01625"
     CDS             6672..>6859
                     /locus_tag="AC162A_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009813363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009563.1"
                     /translation="MSIGNRPPGGHVRADGRAPTDLRPIEITLGVQKWAEGSCMIRFG
                     DTQVLCAATIEDRVPPHLR"
BASE COUNT         1089 a         2426 c         2462 g          882 t
ORIGIN      
        1 gacctgcttg ccctcgccgc tcaccgaccg ctggtagcgg ttcgtggcgc ggttgtactt
       61 gtaggtgatc ttgttcgcga ggtaggggac ggagatggtg ccgccctcgg gacgctcctc
      121 gagcggcacg gggtcgccga acttccagac gggcttctgg cccgggacgg gatcagcccc
      181 gacccgcttg gcgagccgac ggaggtgctt gccgtccgtg tacacgttgt gcggggcgaa
      241 gcgggtgttg atccgccaca ggtagcggcc gccgccccag cggaactcat cggcgttgta
      301 gacgtaggag ccccgtccct tggacgaggc gagtagggcc ttcgcctgcg gcgcgccgcc
      361 cgcgtggacg tacagcgacc gccactcgga cgcccaggcg atgaagtaca gccgcgagct
      421 gcggaccggc ccgaccgcgg gcgggtcgcc tgtctggaag aaggccatgt agcgcgggat
      481 gccgccctcg gcgggtgcct gccacacgac gtcggcgtcg gacagaccgg actgcggccg
      541 ggcgtcgaac tggtcgtcga tcatgaccgc caccacgcgg cgctcagcct gcgcgggctt
      601 gacgggcatg ccggtgagcg gcgccggagc ggtgacgggc gtcgggaccg gggtcggcga
      661 cggcgatgcc gacggctccg tgctgggggg cggctcagcg ctcgagccgc cgggctctgt
      721 cggcaccggc gagccgccgg gcgaggaggg cgttgtgggg tcgggcggag gcgccgtcac
      781 gccggcgatc ggcgtggacg tcgcgccggg cgcggcggtc agcatgctcg cggcaccgat
      841 gccgacgagc gcgccgacga cgaggaccac cacgacgatg gcgccggttg cactccggct
      901 cacgaggcga accctcgctc acgaccctgt ggacggtgca tgtacatccc cggccgcggt
      961 tcttggtcac gcgaccccgc gtcggctaca aggccagacc cggtcgggcg ggaggatagc
     1021 cgaacttgtc gcgacatgcc ccccgcgggt gcccgacact gccggcgtgg ccgtccacca
     1081 ccgttggggt aggctgcgcc gtagtccgtc tgccagcccg cgaacgcccg aggttcgacc
     1141 cgatgcccga cgacacgccc accccgccga agcgcacgcg caagcggtcg acccggaagc
     1201 cctccccggc cgagctggtc ggggtgatgg cgcccgatcc ggagccggat ccggggctgg
     1261 tcgccgtcga ggaggcgggc gaagccgatg cagcggcccc cgcggcggac gaagcagcaa
     1321 cggtcgccga tgcggccgga tccgccgcgg cgccggagga gcccacgggc ggcggcgcgc
     1381 ccgcggcgag ccccgggctc cccgacacgg tgctccggat cgagcgcggg gggatcggcg
     1441 aggcaacggc tggcagcgtg gaggtccacg tcggtgggat cggtgcgctc gacgcggagg
     1501 acgtgttcgt ccagtggggc ggcgtcggtg cagcacgagc cgaccatctc gccgtcgagt
     1561 tcgggagcgt cggagcggcg ctggccggcg agatgcggct gacacagggc ttcgccagcg
     1621 ccgttgccgc gcgcgaggcg acgatcgagc aggcgctcgt ccggacgctc atcgcgcagg
     1681 aggtcacgat caatcgcccg accggcgtcc tggtgatgat cgcgcagcgc gtatcgggcg
     1741 acgtccgtcc ggtcctcgac tggcgaggcg cgctcgcggc gggggccgcg ttcgcgctca
     1801 tcaccgcgct gattcgcggg ctgcgcgagg cgcgcgaccg aagctgagcg gccgattggc
     1861 cgcgaccgag gcgccgtcag cccttccggc gacgaccgcc gctgcccggc gtccgggtcc
     1921 gcaggcgcag cacgcccttc tccttcgcct cggcgcgccg ctcggccatg tgctccttgt
     1981 ggagctcgac gacctcgtcg ggcgagccga agtgggcgat gcgccccttc tcgatcagga
     2041 tggcccggtt gcagtactcg gtcacccagt tcatgtcgtg ggtgacgagc acgatcgcct
     2101 tcgcctcgcg gacaagctcc agcactcgcg ccttcgactt ctcgcggaac gccgcgtcgc
     2161 ccgtcgccag cacctcgtcg agcagcagga tgtcgggctc gacggaggtg gcgatcgaga
     2221 acccgaggcg cgcgcgcatc ccggccgagt aggtcttgat cggggcgtcg atgaactggc
     2281 cgatctcggc gtactcgatg atcccgggca ggcgctcctc gacgacccga tggtcgatgc
     2341 ccatgaacgc gccggcgagg cggatgttgt cgacgccgct caggtcctcg tcgaagccga
     2401 cccccagcgt cagcaggctc gagatgtggc cgtcgacctc gaccacgccc tccgacggga
     2461 cgaggatccc cgccagcacc tggagcagcg tgctcttgcc cgccccgttg ggtccgatga
     2521 cgcccaccga ctcaccctgg agcaccgtga acgacgcgtc gcgcagcgcc cagaagtcgg
     2581 ggtcgccgac ccggcgcacg cggtggacga tcgagtcgcg gatggtctgc ttcctggtga
     2641 ggtgcaggct gtagcggaca ccgagccgct cggcccggat gacgacgcgg tcgctggcgg
     2701 ggacggtcac gcgatcggcg gccacggcgt cacaggacct tcgcgaacga cggttccgcc
     2761 cgcttgaaca ggtacaccgc gatcagcacc atcacgaccg acacggcgag caggatggcc
     2821 agcccggtcc agtgcggagc ccgcccgtag aagatgaggt cgcggtacgc cccgaacagg
     2881 acggtccacg ggttcagggc gaacaggtcg gcaatgatcg ggttgtcctt ggcgatctcc
     2941 ttgaccagct cggccgggta gagcgcaggc gacaggtaga accacatccg cagcaggtgg
     3001 cgcgtgaggt tgccgacatc gcgatagaac acgttggccg cggagacgag gatgacgacg
     3061 cccagcgaga acaccagctg cacgacggcg accagcggga tcagcaggaa ccaggcgctc
     3121 aggtgcccgc ggtagaagat gagcgtgatt gcgagcagcg ggacgaggcc gaacgcgaag
     3181 ctcacgaccc cggccatcgt ggtggacacg ggcagcacga gcttggggaa gaagatctgc
     3241 ttgatcagcc gctcgcggct cacgatcgag ctcacgccat cgccgaccgc cgactggaac
     3301 cacttccagg gcaggatcgc gcacagcacg aacagcgggt agtcctcgat cgccccccca
     3361 cgctgcagga tgatgcccac gaagatcacg tagacgagca tctgcagcag cgggtccatg
     3421 aaccaccaca ggttgcccag cagggtgtcg gacccggact tgcgcaggtc ggctcgaacg
     3481 aggtaggcga tcagccgccg ccgggacagg acctcgcgga tgccgccctg taccagggag
     3541 agggctccgg gacgaaccga cgacgaccga aacgaggcag gcgcctcaga tgtggcggtc
     3601 acggccggga gggtagcacg cgggtgcacg ggtcaccccc ggctccgtag ggggacttgg
     3661 ggtaccattg cgcgccgcgc cgccagcgaa ctctcccgtc gccgcgacgg ccgccaccac
     3721 gaggatccac cgacccgatg tcgttccgcg cacggccgaa ctccaagcac tcacgcacgt
     3781 gggacgaacg agaccggcgt tcaatgctgc tcaatgtcgg cttcggcctg acgatcgcca
     3841 tcgcgctgct gctcctcctc gtcgccgtcg ggtactcctg gtacaacgac cacctgtcgc
     3901 cggtggggag cgtcaacggg cagtcgatct cgaaggacgc gttccgcaag caggttgcga
     3961 tcaacgactt ccgcgcggac taccagaatc gacgcatccg cacgctgctg accgcgggtc
     4021 acctgcggac cgccgacgcg caggcgcggc aggccgtcct cgaccagcgc aaccagcaga
     4081 aggccacgat ctcgctcgag caactgatcg atggcgcggt catggcccag ctcgcaacgt
     4141 cgaagaacgt cacggtcgcc gacgccgacg tcgacgcgcg gctggccgag gacgcgacca
     4201 cgcccgagct gcgtcacgcg tggatgatcg ccgtcgcacc cgagctcgcc gagggcgaga
     4261 cgaaggcgac cgacgcggag aaggcggccg ccaaggccaa ggccgaccag gcgctcgccg
     4321 acctgcgggc cggcaaggac tgggagacgg tcgccaaggc cgtgtcgacc gacggcacca
     4381 agaaccaggg cggggacatc ggctatatcg acgagaatgc ggcgctcgac ccgccgttcg
     4441 tcgaggcggt gatggccgcc gcgctgaacg cgacgaccga cgtgatcgaa ggcgccgacg
     4501 gcacgtaccg gatcgggcgc gtgacggaca tcgtcgcgcc ggtcgtcgac gccacctacc
     4561 ttgaccaggt caaggacgcg ggcatcgacc tcagcgacta ccgcgaggcg ctccggcgcg
     4621 acgtgctgcg gacaaagctg agcgacgcgg tggtcgcgca gtacctggca cccgggccgc
     4681 agcgcgaggt ctcggagatc cacctcgggg tcgacgtcga tccgagcacc ggcagcccga
     4741 cgggcaagga gtcggacccg accgccgtca agatccgcca catcctgtac tcgcccgccg
     4801 gcgacgcgca ggcggcggcg tcgcttgccc ccgacgacgc ggcctggaag gcggccgagg
     4861 agaaggccaa cgcggcctac cagaagctca aggcggaccc gtccctgttc gcctcgctcg
     4921 tcgcctccga cagcgacgac ggcgggtcgt cgagccgcgg cggcagctac tggttcacgc
     4981 ccgacgacgg gctcctgccc gagttccagg aagcgatctt caagcccggc ctgaccccgg
     5041 gccagctcct cgagcctgtg aagacggacg ccggctggca cgtgatccag atccagcact
     5101 tcccgaccga cctggagtgg gcgaacacgc tcaagcagca gatcgacgcc ggcacgctca
     5161 ccttcgccga cgccgctcgc gacaactccg atacctggga cgcagcgacg gccggcgacg
     5221 accagtggat cctcaagggc cagctctccc cggaggtcga ggcggcgctg ttcgcggcgc
     5281 cgatcggcaa gctgagcagt ccgctggccg tcgaaggcga tggcgtgtac ctgttcctcg
     5341 tgtcgcagga acagaaccgc gcccccgacg ccgaacagaa ggcgagcctc gagagctcgg
     5401 cgttcccgac ctggtactcg acccagaagg gcgcctacgc gatcgagcgc gaccccgaaa
     5461 tcaccgcctc cggcgcgtcg ggctgacggc gccgctggcg gccggacgat gctggacgca
     5521 ctcctcgccg aggccaggct ccgatggggc ctcgatccgg ccgcgggcct gtgcgtcgtc
     5581 gttacggagc agctggtcgc cacgcccatc gagccctcga tcgcgcagct gatcgtcccc
     5641 gcggcccgcc tgcgcgccgg gatcccgggc gcaaaccccg caccccttcc cggtcgccac
     5701 ggccatggtg gcgagcccat cgagaccttg cggcgcctct acccggccgg ccatccggtc
     5761 ggccggttcg gacagcccga cgggacaacg ctcgccgatc tcaccgaggg cgacctcggc
     5821 tccccggtct acctcgcccc cgtcgccccc gtcgccgacc tcgcctcgcc gtggggcatg
     5881 ccctggatct ccgctcggct gcgcgagccg gacggctgcc cgtgggaccg cgagcagacc
     5941 cacgcctcgc tccgcaacca cctcctcgag gaggcgtacg aggtctacga cgcgctcgac
     6001 gacggcgcga cccccgagct ggcgggcgag ctcggcgacc tgctcctcca ggtcgtgctc
     6061 cacgcccagc tggccgccga gcagggcgtg ttcgacatga ccgacgtctg gtcggccatc
     6121 gcgtccaaga tcgtccaccg tcacccgcac gtgttcggtg atgccgaggc gcgcacggcc
     6181 tccgacgtca accgccagtg ggagcggatc aaggcgggtg agcgcgcgga ggccgcctcg
     6241 gggtccggcg acggagccga cgtggcgccg acgagcgccc tcgacggcat cagccgcacc
     6301 ctgccggcgc tggccgcgag ccaggagatg caggaacgcg ccgccaacct gggctacgac
     6361 tggccgtcga tcgacggggt cctggacaag gtgcgcgagg aggtcgggga gctggtcgag
     6421 gccgaggacg ccgcccaccg gaccgaggag ctgggtgacc tgctgttcgt cctggtcaac
     6481 gtcgggcgca agaccgggat cgaggtcgag ggcgcgctgc gcggggccaa cgagaagttt
     6541 cgcggccggt tccggcacgt ggagctggcc gccgccgcgc gcaacgtcgc gctgcgggac
     6601 atgtcgttcg aggagttgga tgcgctctgg gatgccgcca aggcggcgga gcgcggggag
     6661 gcgaggacat catgagcatc ggcaatcggc cgcccggcgg ccacgttcgc gcggacggtc
     6721 gcgcgcccac ggacctgcgt ccgatcgaga tcacgctggg cgtccagaag tgggccgagg
     6781 gctcgtgcat gatccggttc ggcgacaccc aggtgctgtg cgcggcgacc atcgaggacc
     6841 gcgtgccgcc gcacctccg
//
LOCUS       DBNHXO010000075         4430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2400048, whole genome shotgun sequence.
ACCESSION   DBNHXO010000075 DBNHXO010000000
VERSION     DBNHXO010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2400048"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="AC162A_01630"
     CDS             <1..1067
                     /locus_tag="AC162A_01630"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019637004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR5009564.1"
                     /translation="ARALEISSARILILDEPTSSLDAHETEQLFGVMRKLKRQGLAVV
                     FITHFLDQVYAVSDRITVLRNGSLVGTFATSQLTRIELIKKMLGRELTELDDMARVKL
                     SGSRGGDGTTRLVASGMGRAGSIEPFDLELHAGEVLGLAGLLGSGRTEIADLLFGVER
                     PDTGTLTVDGTPVGKFSPRASIARGVALCPEDRKAEGIVDELTVRENIMLAVQAGRGW
                     LRYLSRAEQDDIADEYIRLLKIATPDAEQLAGHLSGGNQQKVILARWLATHPRILILD
                     EPTRGIDIGAKAEVQRLVLALAEDGTSCVFISSELEEVLRTCDRIVVLRDRVKVTEFE
                     GDVDEGVIMHAMAGVEHGVD"
     gene            1054..2112
                     /locus_tag="AC162A_01635"
     CDS             1054..2112
                     /locus_tag="AC162A_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009565.1"
                     /translation="MASTRSVSLVRRIGESKVLFPTIALGLILIFDLLTVPGFFGLQT
                     IDGHLYGNLVDIPRNGSTIMLLALGMTLVIATGGVDLSVGAVMAISAAVAALLINPDL
                     NSLNHDPNVTQTPLWAVVLITLAVATLCGAWNGMLVAYAGIQPMVATLILMIAGRGIA
                     QLISNGVRIQIFYEPYAVFGNGWLVLPVSLYVVAVVFTVAWLFTRRTALGTFIEAVGI
                     NARSSYYSGIDEKRVKLVAYMFCGLCAGMAGIVASSNIRTSDANNIGLNFDLDAILAV
                     VIGGTALGAGGRFSLLASLVGALVIQAITTSMYAVGVPASGLLAIKGFVVIIVILLFS
                     VQVRDWVRSVAMRRGTVQ"
     gene            2109..3188
                     /gene="yjfF"
                     /locus_tag="AC162A_01640"
     CDS             2109..3188
                     /gene="yjfF"
                     /locus_tag="AC162A_01640"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HIR5009566.1"
                     /translation="MNARFLDRLGRYRRFAPLATTITLFCLAYAYGALAYESMRDPQV
                     FLNLLRAAPFLLISAIGMTFVILSGGIDLSVGGVVALTTVVSAALLRNNGTDPWLVMV
                     IVLVMGMAFGLVMGFFITYLKVQPFIATLAGLWVARGLCFFISDNAIPIRNRLYEILA
                     GTKLLIPGLSDPAAKTGDFVSIPAVFSLLFLALMMIIAHKTGFGRSVYAIGGNELSAR
                     LMGLPVNRTKMAVYTLNGLCSALAGIALSIDVGSGHGLYASGMELTVIGAVVIGGTLL
                     TGGVGYVFGTLFGVLIIGVIQTLIQFNGTLSSWWTPIVVGALTLVFIGVQSFLAGQGP
                     FRRSRAITSSATSGPSGELPEGEAP"
     gene            3188..4099
                     /locus_tag="AC162A_01645"
     CDS             3188..4099
                     /locus_tag="AC162A_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009567.1"
                     /translation="MRVGRTAALIACAIAIAGCEYVVVPPEASGQAAVSGEGWTAVPT
                     SVGPTDGGALGIDLTIRNDTGAWSAMEAAAKPATLTGADGATSTCATVKVGTGGHRVA
                     PGLQIRGYQTGPKKAPVTELIRVECDGATAAAGAKVALEYVYVTGEYNYYDSDATRTA
                     ATLEVPLEPLAADLSYPIGETVDGLVQPADIEMTAINGMVLTLVATRRTDTGLAFDWQ
                     ATNPGEYPSSVHIGTPPVVGADGIVYGFYESPDLASVPVTDAGATSEWTTEVAVPDDV
                     TGLYMLLSVENKKQRLFTNYLLDLGTP"
     gene            4123..>4430
                     /locus_tag="AC162A_01650"
     CDS             4123..>4430
                     /locus_tag="AC162A_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HIR5009568.1"
                     /translation="MKSLGQHEVWFVTGSQDLYGPDALRQVAENAAAVVAALDASSAI
                     PVRVVFRPVVASPEAIAATCLEANANDACVGVIAWMHTFSPARMWIAGLQALAKPLLH
                     L"
BASE COUNT          633 a         1554 c         1527 g          716 t
ORIGIN      
        1 tcgcgcgggc gctggagatc tcgtccgccc ggatcctcat cctcgacgag ccgacctcga
       61 gcctggacgc ccacgagacc gagcagctgt tcggcgtgat gcgcaagctc aagcgccagg
      121 gcctcgcggt cgtattcatc acccacttcc tcgaccaggt gtacgccgtc tcggaccgga
      181 tcacggtcct gcgcaacggc agcctcgtcg ggacgttcgc gacctcccag ctgacgcgca
      241 tcgagctgat caagaagatg ctcgggcgcg agctcaccga gctcgacgac atggcccgcg
      301 tcaagctctc gggctcccgc ggcggcgatg gcacgacgcg cctcgtggcc agcggcatgg
      361 gacgggcggg ctcgatcgaa ccgttcgacc tcgagctcca cgccggcgag gtgctcggcc
      421 tggcggggct gctgggatcc ggtcgcacgg agatcgccga cctcctgttc ggggtcgagc
      481 ggccggatac cgggacgctg accgtcgacg gcacccccgt cggcaagttc tcgccgcggg
      541 cctcgatcgc ccgtggcgtg gcgctgtgcc cggaggaccg caaggccgag ggcatcgtcg
      601 acgagctgac ggtgcgcgag aacatcatgc tggcggtcca ggcgggccgc ggctggctgc
      661 gctacctgag ccgcgccgag caggatgaca tcgccgacga gtacatccgg ctgctcaaga
      721 tcgcgacccc ggacgcggag cagctcgccg ggcacctgtc gggcggcaac cagcagaagg
      781 tcatcctcgc ccgctggctc gcgacgcacc cgcggatcct gatcctcgac gagcccacgc
      841 gcggcatcga catcggcgcc aaggccgagg tgcagcggct ggtgctcgcg ctcgccgagg
      901 acgggacctc gtgcgtgttc atctcgtcgg agctcgagga ggtcctgcgg acctgcgatc
      961 ggatcgtcgt gctccgcgac cgcgtcaagg tcaccgagtt cgagggcgac gtcgacgagg
     1021 gcgtgatcat gcacgcgatg gcgggggtcg agcatggcgt cgactaggtc cgtctcgctc
     1081 gttcgccgga tcggcgagag caaggtcctg ttcccgacga tcgccctcgg gctgatcctc
     1141 atcttcgacc tgctcaccgt cccgggcttc ttcgggctcc agacgatcga cgggcacctg
     1201 tacgggaacc tcgtcgacat cccccgcaac ggctcgacga tcatgctgct cgccctcggg
     1261 atgaccctgg tcatcgcgac cggcggcgtc gacctgtcgg tcggggcggt gatggcgatc
     1321 tcggcggccg ttgccgccct gctcatcaat cccgacctca actcgctcaa ccacgacccg
     1381 aacgtgaccc agacgccgct gtgggcggtc gtcctgatca ccctcgccgt ggcgaccctg
     1441 tgcggggcgt ggaacgggat gctggtcgcg tacgcaggca tccagccgat ggtggcgacg
     1501 ctgatcctga tgatcgccgg ccgaggcatc gcccagctga tctcgaacgg cgtccggatc
     1561 cagatcttct acgagccgta cgcggtgttc ggcaatggct ggctggtgct gccggtctcc
     1621 ctgtacgtgg tcgcggttgt gttcaccgtg gcctggctgt tcacgcggcg gaccgcgctc
     1681 gggacgttca tcgaggccgt cggcatcaac gcgcgctcga gctactacag cggcatcgac
     1741 gagaagcgcg tcaagctcgt cgcctacatg ttctgcggcc tgtgcgcggg catggcgggc
     1801 atcgtcgcca gctccaacat ccggacctcg gacgccaaca acatcggcct caacttcgac
     1861 ctcgacgcga tcctggcggt cgtcatcggc ggcacggcgc tcggcgccgg gggccggttc
     1921 tcgctgctgg ccagcctcgt cggggcgctg gtgatccagg cgatcacgac ctcgatgtac
     1981 gcggtcggcg tgccggccag cgggctgctg gccatcaagg gcttcgtcgt gatcatcgtg
     2041 atcctgctgt tctcggtgca ggtccgggac tgggtccgct cggtcgccat gcgccggggc
     2101 accgtccagt gaacgcccgg ttcctcgatc gcctcgggcg ctaccggcgg ttcgcgccgc
     2161 tggccaccac gatcacgctg ttctgcctcg cctacgcgta cggggcgctg gcgtacgaga
     2221 gcatgcgcga cccgcaggtg ttcctgaacc tgctgcgcgc ggcgccgttc ctgctgatca
     2281 gcgccatcgg catgacgttc gtgatcctgt ccggcgggat cgacctgtcg gtgggcggcg
     2341 tggtggcgct cacgaccgtc gtctcggcag cgctgctgcg caacaacggg acggacccgt
     2401 ggctggtcat ggtgatcgtc ctggtgatgg ggatggcctt cggcctggtg atgggcttct
     2461 tcatcaccta cctcaaggtc cagccgttca tcgccaccct ggccggcctg tgggttgccc
     2521 gcggcctgtg cttcttcatc agcgacaacg cgatccccat ccgcaaccgg ctgtacgaga
     2581 tcctcgccgg cacgaagctc ctgatccccg ggctcagcga cccggccgcg aagacgggcg
     2641 acttcgtgtc gatcccggcc gtcttttcgc tcctgttcct cgcgctgatg atgatcatcg
     2701 cccacaagac cggcttcggg cgctccgtgt acgccatcgg cggcaacgag ctctcggcgc
     2761 ggctcatggg cctgccggtg aaccggacga agatggccgt gtacaccctc aacggcctgt
     2821 gctcggcgct ggccggcatc gcgctcagca tcgacgtcgg gtcgggccac gggctgtatg
     2881 cctcggggat ggagctcacg gtcatcggcg cggtggtcat cggagggacg ctgctgacgg
     2941 gcggcgtcgg ctacgtgttc gggacgctgt tcggcgtcct gatcatcggc gtcatccaga
     3001 cgctcatcca gttcaacggg accctctcct cgtggtggac gccgatcgtg gtcggcgccc
     3061 tcacgctcgt gttcatcggc gtccagagct tcctcgcggg ccagggcccg ttccggcggt
     3121 cgcgcgcgat cacgtcgtcg gcgacctcgg gcccgtcggg cgagctgcca gagggggagg
     3181 cgccgtgatg agggttgggc gcacggccgc gctcatcgcg tgcgcgatcg cgatcgccgg
     3241 ctgcgagtac gtggtcgtgc cgcccgaggc cagcggccag gcggcggtca gcggcgaggg
     3301 ctggacggcc gtcccgacga gcgtcggccc gaccgatggc ggcgcgctcg ggatcgacct
     3361 gacgatccgc aacgacacgg gggcctggag cgcgatggag gcagccgcca agccggccac
     3421 cctgaccgga gcggacggcg cgacctcgac ctgcgcgacg gtcaaggtcg gcaccggcgg
     3481 ccaccgcgtg gcgccggggc tgcagatccg cggctaccag acggggccca agaaggcccc
     3541 ggtgaccgag ctgatccgcg tcgagtgcga cggcgcgacg gcggccgcgg gcgcgaaggt
     3601 tgcgctcgag tacgtctacg tgaccggcga gtacaactac tacgactcgg atgcgacgcg
     3661 caccgccgcg acgctggagg tcccactcga gccgctcgcg gcggacctca gctacccgat
     3721 cggggagacg gtcgacggcc tcgtccagcc ggccgacatc gagatgaccg cgatcaacgg
     3781 catggtgctg acgctcgtcg ccacccggcg cacggacacc gggctcgcct tcgactggca
     3841 ggccaccaac ccgggcgagt acccctcgtc ggtccacatc ggcacaccgc cggtcgtcgg
     3901 ggccgacggc atcgtctacg gcttctacga gagccccgac ctggcctccg tgcccgtcac
     3961 ggacgccggc gccacatcgg agtggaccac ggaggtcgcc gtccccgacg atgtgaccgg
     4021 tttgtacatg ctgctgagcg tggagaacaa gaagcagcgg ctgttcacca actacctgct
     4081 ggacctcggc accccatgaa cgcgacagac aacggaggca acgtgaagtc gctcggccaa
     4141 cacgaggtgt ggttcgtcac cggcagtcag gacctgtacg gcccggacgc gctgcgccag
     4201 gtcgcggaga acgccgcggc cgtggtggcg gccctcgacg cgtcgtccgc gatccccgtt
     4261 cgggtggtct tccggcccgt cgtcgcctcc cccgaagcca tcgcggcgac ctgcctcgag
     4321 gcgaacgcga acgatgcgtg cgtcggcgtc atcgcctgga tgcacacgtt ctcgccggcc
     4381 cggatgtgga tcgccggcct ccaggcgctg gccaagccgc tgctgcacct
//
LOCUS       DBNHXO010000076         2411 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_614125, whole genome shotgun sequence.
ACCESSION   DBNHXO010000076 DBNHXO010000000
VERSION     DBNHXO010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_614125"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1545
                     /locus_tag="AC162A_01655"
     CDS             154..1545
                     /locus_tag="AC162A_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5009569.1"
                     /translation="MTGAGVDFDALDRLVDARLERWTAELVEYVAISSEGGDLPGLRA
                     AADWTADRLRRLGASVEVLEIEGRPPLVVGEIGGGPRTLNAVQHYDVQPATPLDLWTT
                     PPYEPAIRDGRVWGRGATDNKGEFLPRLWAVEAWLEAIGPLPCRVRFLVEGEEETGSH
                     TLDTLLDLRPELREADAALIEGGGLDLAGRPYVDGGGKGIVVVELHLRTMKVDAHSSG
                     SVVLPNAGHRLVAALATFWNADGRPAIAGLEAGKRAPTPTQLAVLAAQDLGWLDDIRQ
                     DAGIETFIGGLDGLAALEALTFETTLNLQGIWAGHAGTTPKTVTPAEAHARLDIRIVP
                     DQMPDEMVTAIRRHLDDGGFADIEVVALEGEAAWWTPADNPLIDAAAAASTDVTGLET
                     IVGLSMAGTVPMFQICATHRVPATTLGAARDDCRAHAPDENIRIEDLGTAARITARFL
                     DRFARLTEIPRVP"
     gene            complement(1555..1899)
                     /locus_tag="AC162A_01660"
     CDS             complement(1555..1899)
                     /locus_tag="AC162A_01660"
                     /inference="COORDINATES: protein motif:HMM:NF015735.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR5009570.1"
                     /translation="METWIYFIHPPREDFAETMTDVESEAWGRHWERIKRLFDEGHIV
                     LIGPTLGRHNTGICVFEAPDEAAALAFMNGDPAIAEGFAKGELRPFQLSLLRGRDEAR
                     IRASLEAAAADG"
     gene            complement(1901..>2411)
                     /locus_tag="AC162A_01665"
     CDS             complement(1901..>2411)
                     /locus_tag="AC162A_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5009571.1"
                     /translation="AGADMGPHGVSIGVMSKSFALAGLRIGWLATRDARLLDAAARFK
                     DYTTICASAPAEVLALIALRARDRVLGRSRAIVEANMALLDGFFERQGDRFTWVRPRG
                     GSIAFPSLVGPVPIERFTQDLLDARGVLLAPGSIFGHPGNHFRIGFGRETMPAALAEL
                     EAFAARTLG"
BASE COUNT          357 a          897 c          838 g          319 t
ORIGIN      
        1 cctcaagcag gccatcgccc tggcctcggt cgcgccgcgg ttcgagcggg tcggcaccag
       61 gctccaggtc gagtggacgg tcgagggcaa gcgcggccgc gtcaacgcca cggtcgttga
      121 gctgccggtc ctcgacctcg aacgcaagcg cgcgtgaccg gcgccggcgt cgacttcgac
      181 gccctcgacc gcctcgtcga cgcgcggctc gaacgctgga cggcggagct cgtcgagtac
      241 gtcgcgatct cgtccgaggg cggcgacctg ccgggcctgc gcgccgccgc ggactggacg
      301 gcggaccggc tccggcgcct gggcgccagc gtcgaggtcc tcgagatcga gggcaggcca
      361 cccctggtgg tcggcgagat cggcggcggg ccgcgaacgc tgaacgccgt gcagcactac
      421 gacgtccagc cggccacgcc gctcgacctc tggacgaccc cgccgtacga gccggccatc
      481 cgcgacggtc gcgtctgggg ccgcggcgcg accgacaaca agggcgagtt cctgcctcgc
      541 ctgtgggccg tcgaggcatg gctcgaggcc attggtccgt tgccctgtcg cgttcggttc
      601 ctcgtcgagg gcgaggagga gaccggcagc cacaccctcg acaccctgct cgacctgcgt
      661 ccggagctcc gcgaggcgga cgccgcgttg atcgaaggcg gcgggctcga cctcgcgggg
      721 cggccatacg tcgatggcgg cggcaagggc atcgtcgtcg tcgagctcca tctgcgcacc
      781 atgaaggttg acgcccactc gagcgggagc gtcgtgctcc ccaacgcggg gcatcggctg
      841 gtcgccgcgc tggcgacgtt ctggaatgcg gacgggcggc cggcgatcgc ggggctcgag
      901 gccggcaagc gggcgccgac cccgacgcag ctcgccgtgc tcgccgccca ggatctcggc
      961 tggctcgacg acatccgcca ggatgccggg atcgagacct tcatcggcgg ccttgacggt
     1021 ctcgcggcgc tcgaggcgct cacgttcgag acgacgctca acctccaggg catctgggcc
     1081 ggccacgcgg gcacgacccc gaagaccgtc actcccgccg aggcgcatgc acgcctcgac
     1141 atccggatcg tcccggacca gatgccggac gagatggtca cggcgatccg ccgccacctc
     1201 gacgacggcg ggttcgcgga catcgaggtc gtcgcgctcg aaggcgaggc cgcctggtgg
     1261 acgcctgccg acaacccgct gatcgacgcg gcggcggcgg catcgaccga cgtcaccggc
     1321 ctcgagacca tcgtcggcct gtcgatggcg gggacggtcc cgatgttcca gatctgtgcc
     1381 acccatcggg tgcccgcgac aaccctcggc gccgcccgcg acgactgccg cgcccatgcg
     1441 cccgacgaga acatccgcat cgaggacctt gggaccgcgg ctcggatcac cgcccgcttc
     1501 ctggatcgat tcgcgcggct gacggagatc ccgcgggtcc cctagccgca cgtgtcagcc
     1561 gtcggcggcg gccgcctcca gcgaggcacg gatccgcgcc tcgtcgcgtc ctcgcagcag
     1621 cgacagctgg aacggccgca gctcgccctt ggcgaacccc tccgcgatcg ccgggtcgcc
     1681 gttcatgaac gcgagtgccg cagcctcgtc gggcgcttcg aacacgcaga tcccggtgtt
     1741 gtggcggcca agcgtcggcc cgatgagcac gatgtgtccc tcatcgaaca ggcgcttgat
     1801 ccgctcccag tggcggcccc acgcctccga ctcgacgtcg gtcatcgtct ccgcgaagtc
     1861 ctcgcgcggc ggatggatga agtagatcca ggtctccatg tcaacccaag gtacgcgccg
     1921 cgaacgcctc gagttcagcc agggcggccg gcatcgtctc tcggccgaac ccgatccgga
     1981 agtggttgcc cggatggccg aagatcgagc ccggggcgag gaggacaccc cgcgcgtcga
     2041 gcaggtcctg cgtgaaccgc tcgatcggca ccggtcccac gagcgaggga aacgcgatcg
     2101 agccgccccg tggcctgacc caggtgaacc gatcgccttg gcgctcgaag aagccgtcga
     2161 gcagcgccat attggcctcg acgatcgccc gcgaccgccc gagaacccgg tcgcgcgcgc
     2221 ggagcgcgat cagcgccagg acctccgccg gggcggacgc gcagatcgtg gtgtagtcct
     2281 tgaaccgggc ggcggcgtcg agcagccggg cgtcgcgcgt cgccagccac ccgatccgca
     2341 agcccgccag cgcgaacgac ttcgacatca ccccgatcga gaccccgtgc ggccccatgt
     2401 ccgcgcccgc c
//
LOCUS       DBNHXO010000077         3753 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_19509, whole genome shotgun sequence.
ACCESSION   DBNHXO010000077 DBNHXO010000000
VERSION     DBNHXO010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_19509"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /gene="surE"
                     /locus_tag="AC162A_01670"
     CDS             <1..618
                     /gene="surE"
                     /locus_tag="AC162A_01670"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014077.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR5009572.1"
                     /translation="FDLVASGINYGANLGDDITYSGTVSAAMEAVINNCPAFAISQEY
                     HEQIDFALATLAAAVVARNILEHGLKRGELLSVNVPAVPIAECEGFEMTRVGRRVYQD
                     QLVSRVDPRGIPYYWLGGPPPSGIRVPGTDFNAVVNRRVSVTPIHLDLTGRRLLRQLQ
                     TWDWPMPDAEGFVPPAAELVTGPPSSAADAAEEQRIAEESQLERR"
     gene            503..1132
                     /locus_tag="AC162A_01675"
     CDS             503..1132
                     /locus_tag="AC162A_01675"
                     /inference="COORDINATES: protein motif:HMM:NF039485.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HIR5009573.1"
                     /translation="MRRASCRPPRSWSPGPRRAPPTRPRSSASPRSRSSSADRRLAGH
                     GTLEPRHSQRGTEMDDIGKMLGGSGGSGVGDIAGAIGGLVGGEGGLQGLVNQLDQAGL
                     GDTVSSWISTGANKPVDPQALGNALGPDKVQQLSGQSGLDVGSLLPILAAALPTVIDA
                     VTPDGNVPQGNAAAGLDVGGMLEGLSEAANAGPNSPLAGLGALLGGPKS"
     gene            complement(1129..2736)
                     /locus_tag="AC162A_01680"
     CDS             complement(1129..2736)
                     /locus_tag="AC162A_01680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR5009574.1"
                     /translation="MATTYDAIVVGAGHNGLTAAAYLAAGGLDVLVLEARDRIGGATV
                     TEELAPGVRVPALAHTVGRLRPSVARELALTKHGLSLVSPEVRVFAPQPDGRAVTLWS
                     DLGKSVDAIRTWSENDAMAFVEFDRGVRSLARFLADLGDEAPPEIRAPGFGDALLGLR
                     LGRAFRGLGKTDGRTILRVLAMAVADYVAESFTSEPLRATLAWRGVRYTAMGPWSAGT
                     TAVLLADSAGNDGGAAGETVFAKGGPSALADALASAAQAAGAEIRTSARVVAVTTVDG
                     VATGVALESGEEILASAVVSGLDPKQLLTGLIDPVTLGPSLRWRAGNIRTPGTLAKVN
                     LVLDGLPEFPAAGGDARLLRGRIQVGTTSIDDVERAFDASKYGRVSERPILEATIPSL
                     VDPSLVAGAKDGTQVMSVLMQWLPAKVAAEDWAARRDELGDLAVATLETVSPGLGARV
                     TARQVLTPGDLETDYGLTGGHPLHAEPALDSFFLWRPLLGWARYRMPVEGLYLAGSGA
                     HPGGGVTGVPGRNAAREVIADRKRRRR"
     gene            complement(2738..>3753)
                     /locus_tag="AC162A_01685"
     CDS             complement(2738..>3753)
                     /locus_tag="AC162A_01685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR5009575.1"
                     /translation="LKATMSASGIIGTYQGIRSPGTAYVLLHHYMGEIDGAFRAWGIP
                     KGGTGGVSYAIARSAQALGAEIRTEAPVARITVRSGRATGVVLESGEEIEASVVLSSA
                     DAKVTFLDLLEDGTLEPEFEQEVRRFKFRGSSGKVNLAVDALPDFSCLPGVGEHLRGA
                     ISFSPSMTDMEQAYDDAKYGHWSRKPYIDMIIPTLVDPQMAPPGKHVISCFVQYAPYK
                     LDPSLGTWDDNREAFGDAVIDRIAEFAPNIRDIIIGRQVLTPLDIERTIGLTEGNIFQ
                     GELSLEQLFFNRPVPGYARFRTPIKDLWLSGSSTHPGGGLMGANGRLAALEVLRARGR
                     KVA"
BASE COUNT          541 a         1378 c         1337 g          497 t
ORIGIN      
        1 ttcgacctcg tcgcctcggg catcaactac ggcgcgaacc tgggcgacga catcacctac
       61 tcgggcacgg tgtcggccgc gatggaggcg gtcatcaaca actgcccggc gttcgcgatc
      121 tcgcaggagt accacgagca gatcgacttc gcgctcgcca cgctggccgc cgcggtcgtc
      181 gcccgcaata tcctcgagca cggcctcaag cgcggggagc tgctcagcgt caacgtgccg
      241 gcggtgccga tcgccgagtg cgaggggttc gagatgaccc gggtcggccg gcgcgtgtac
      301 caggatcagc tggtctcgcg cgtcgatccg cgcggcatcc cctactactg gctcggcggc
      361 ccgccgccgt ccggaatccg cgtgcccggc accgacttca atgcggtggt caatcgccgg
      421 gtctcggtca cgccgatcca cctcgacctc accggccgcc gcctccttcg gcaactccag
      481 acgtgggact ggccgatgcc cgatgcggag ggcttcgtgc cgcccgccgc ggagctggtc
      541 accgggcccc cgtcgagcgc cgccgacgcg gccgaggagc agcgcatcgc cgaggagtcg
      601 cagctcgagc gccgataggc gccttgccgg ccatggcact ctggagcctc gacactcgca
      661 gcggggaacg gagatggacg acatcggcaa gatgctcggg ggctcgggcg gctcaggggt
      721 tggcgacatc gcaggcgcga tcggcggcct cgtcggtggc gagggcggcc tccaggggct
      781 ggtgaaccag ctcgaccagg cgggtctcgg cgacacggtc agctcgtgga tctcgaccgg
      841 cgccaacaaa ccggtcgatc cccaggcact gggcaatgcg ctcggcccgg acaaggtgca
      901 gcagctatcg ggccagagcg gcctcgatgt gggctccctg ctcccgatcc tggcggcggc
      961 cctgcccacc gtgatcgacg cggtcacccc cgacggcaac gtaccccagg gcaacgccgc
     1021 ggccggcctc gatgtcggcg gcatgctcga gggcctgtcc gaggccgcaa acgctggccc
     1081 caactcgccg ctggccggtc tgggcgcgtt gctgggcggc ccgaagagct agcggcgccg
     1141 gcgcttgcgg tcggcgatca cttcgcgagc ggcgttgcgc cccgggacgc ccgtgacgcc
     1201 gccgccaggg tgtgccccgg agccggcgag atacaggcct tcgaccggca tccggtagcg
     1261 cgcccagccg agcagcggcc gccacaggaa gaacgaatcg agcgcgggct cggcgtgcag
     1321 cggatgaccg ccggtcagcc cgtagtcggt ctccaggtcg ccgggtgtca ggacctgtcg
     1381 tgccgtcacg cgggcgccca ggcccggcga gacggtctcg agcgtcgcga ccgcaaggtc
     1441 gcccagctcg tcgcgacgcg ccgcccagtc ctcggcggcg accttcgcgg gcagccactg
     1501 catcaggacg ctcatcacct gagtcccgtc cttcgccccg gcgaccagcg atgggtcgac
     1561 gagcgacggg atcgtcgcct cgaggatcgg gcgctccgag acgcggccgt acttcgaggc
     1621 atcaaaggcc cgctccacgt cgtcgatcga cgtcgtcccg acctggatcc ggccgcgcag
     1681 cagccgggca tcgccgccgg cggcggggaa ctccggcagg ccgtcgagga cgaggttgac
     1741 cttggccagc gtcccgggcg tccggatgtt gcccgcccgc cagcgcaggg acggcccaag
     1801 ggtgacgggg tcgatcagcc cggtgagcag ctgcttcgga tcgagaccgg agacgaccgc
     1861 cgacgcgagg atctcctcgc ccgactccag cgcaacgccg gtcgcgacgc catcgacggt
     1921 cgttaccgcc acgacgcgcg ccgaggtgcg gatctcggca cccgcagcct gggccgccga
     1981 cgccagcgca tcggccagcg cggacggacc gcctttcgcg aacaccgtct cgcccgcggc
     2041 gccgccatcg ttgccggccg agtcggccag caggaccgcc gtcgtgcccg cggaccaggg
     2101 gcccatcgcc gtgtaccgga cgccgcgcca ggcgagcgtc gcgcgcagcg gctcggaggt
     2161 gaacgactcg gcgacgtagt cggccacggc catcgcgagg acgcgcagga tcgtgcggcc
     2221 atcggtcttg cccaggccgc ggaacgcgcg ccccagccgc aaccccagca gcgcgtcgcc
     2281 gaagccgggc gcccggatct cgggcggcgc ctcatcgccg aggtcggcca ggaaccgcgc
     2341 cagcgagcgg acgccgcggt cgaactccac gaacgccatc gcgtcgttct cggaccacgt
     2401 gcggatcgcg tccacggact tgccgaggtc gctccacagc gtcaccgccc gcccgtccgg
     2461 ttgcggcgcg aagacgcgga cctccggcga gaccagcgac aggccgtgct tggtgagggc
     2521 gagctcgcgg gcgaccgagg gccgcaaccg gccgaccgtg tgagcgagcg ccggcacgcg
     2581 cacgccgggc gccagctcct cggtcacggt cgcgccgccg atgcggtcgc gcgcctcgag
     2641 caccagcacg tcgaggccgc cggcggcgag gtacgcggcg gcggtcaggc cgttgtggcc
     2701 ggcgccgacg acgatcgcgt cgtaggtggt cgccacctca ggccaccttc cggccgcggg
     2761 cccgcaggac ctcgagggcc gcgagccggc cgttcgcgcc catcagcccg ccgcccggat
     2821 gggtggacga gcccgacagc cacaggtcct tgatcggggt cctgaaccgc gcgtaacccg
     2881 ggaccgggcg gttgaagaac agctgctcga gcgacagctc gccctggaag atgttgccct
     2941 ccgtcaggcc gatggtccgc tcgatgtcca gcggggtcag cacctggcga ccgatgatga
     3001 tgtcgcggat gttgggcgcg aactccgcga tccggtcgat caccgcgtcg ccgaacgcct
     3061 cgcggttgtc gtcccaggtg ccgaggctcg ggtcgagctt gtacggcgcg tactgcacga
     3121 agcagctgat cacgtgcttg ccgggcggcg ccatctgggg atcgacgagc gtcgggatga
     3181 tcatgtcgat gtacggcttg cggctccagt ggccgtactt cgcgtcgtcg tacgcctgct
     3241 ccatgtccgt catcgagggg ctgaagctga tcgccccccg caggtgctcg ccgacacccg
     3301 gcaggcacga gaagtcgggc agcgcgtcca ccgcgagatt caccttgccg gagctgccgc
     3361 ggaacttgaa ccgccgcacc tcctgctcga actcgggctc cagcgtcccg tcctcgagga
     3421 ggtccaggaa ggtcaccttc gcgtcggcgg aggacaggac gacggacgcc tcgatctcct
     3481 cgccgctctc gagcacgacg ccggtggccc ggccgctgcg cacggtgatc cgggcgaccg
     3541 gcgcctcggt gcggatctcg gcgcccagcg cctgtgcgga gcgggcgatc gcgtacgaca
     3601 cgccgccggt gccacccttg gggatgcccc aggcgcggaa cgcgccgtcg atctcgccca
     3661 tgtagtggtg gagcagcacg tacgccgtcc cgggcgaccg gatgccctgg taggtgccga
     3721 tgatccccga cgccgacatc gtcgccttga gcg
//
LOCUS       DBNHXO010000078         2783 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3392968, whole genome shotgun sequence.
ACCESSION   DBNHXO010000078 DBNHXO010000000
VERSION     DBNHXO010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3392968"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="AC162A_01690"
     CDS             complement(<1..537)
                     /locus_tag="AC162A_01690"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR5009576.1"
                     /translation="MATKIILDVDTGTDDAVALMTAALSPDIELVGASVVNGNTNVDS
                     CTENTLRVFDWIGMPSVPVHRGMSRPLARPQATQLNPATRIHGDLLDLPPASRSADAG
                     HAVDWLIDTYLASDGDITLVPVGPLTNVATAIQKAPRILERIPEIVIMGGAHDHGNAT
                     PSAEFNIWLDPEAARIVVN"
     gene            613..1305
                     /locus_tag="AC162A_01695"
     CDS             613..1305
                     /locus_tag="AC162A_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009577.1"
                     /translation="MILSTSPRAASIASWMRGLVLVFAALFASHDAVYLVRFGPGAGY
                     ANAMSAGGHDGYWLPASLGLVIGASIAFLSVVHVIIRLRRDIGAPPHAAAVTPGGPSY
                     GAELARLWLRLLVAVGLLFAVQENIEGLLANGGIAGLDPLFGHDAWLVPPILAATTFL
                     LAAVGAVLRWRVRILQARVEAARRARAFGRPIASAPSRRWLTMPAGGGHGPDAARPDA
                     GRAPPIVIAAAA"
     gene            1406..2020
                     /locus_tag="AC162A_01700"
     CDS             1406..2020
                     /locus_tag="AC162A_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009578.1"
                     /translation="MNRTRTVRRLGGAVTVAALFSLLAAGTSLAHEVRDVGEYTVVVG
                     FIDEPVFVGQKSGLEFFVDKGDTPVDGLEQTLKAEVIYQDQHRDLPIEARFGEEGAYE
                     SVFFPTAAGPYTFHISGTLDGQAIDESFTSSPEGFSEVEDLASGQFPVQFPSQAEVVS
                     DAQAGRAAQTQATIGIAVGVIGLVVAVVAVGLALSVRRRAPAPK"
     gene            2044..>2783
                     /locus_tag="AC162A_01705"
     CDS             2044..>2783
                     /locus_tag="AC162A_01705"
                     /inference="COORDINATES: protein motif:HMM:NF016149.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0046688 - response to copper ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HIR5009579.1"
                     /translation="MAGALLLAGPILIGPAAPVALAHAELVTSAPAAGDVVATAPAEI
                     RLVFSETIDGAYTRLDLLDPLGHMIADGIGAPDPSDPYTLVAPIEPLPDGLYTVNWQA
                     LSRADGHRTSGFFTFGVGDVAPPAGGGQDAGGSIHAGHDAVTALIETESRFAQLLGLL
                     LAVGLPVFVFTALKIEPSRRLGQAGAGALLIAAVGAFGLLAVNAAGFGTDPVAYARDT
                     RSGQLLAAGALLALAGGLVVLGLARRRSP"
BASE COUNT          335 a          984 c         1006 g          458 t
ORIGIN      
        1 gttgacgacg atccgcgccg cttcagggtc gagccagatg ttgaactccg ccgacggcgt
       61 cgcgttcccg tggtcgtggg cgccgcccat gatcacgatc tccgggatcc gctccaggat
      121 ccgcggcgcc ttctggatcg cggtcgcgac gttggtcagc ggcccgaccg gcaccagcgt
      181 gatgtcgccg tccgacgcga ggtaggtgtc gatcagccag tcgaccgcgt ggccagcgtc
      241 ggcagagcgg gaggcgggtg gcaggtcgag caggtcaccg tggatccggg tcgcggggtt
      301 gagctgggtc gcttgcgggc gcgcgagtgg tcgggacatg ccgcgatgga cgggaacgga
      361 gggcatcccg atccagtcga acacgcgcag cgtgttctcg gtgcagctgt cgacgttggt
      421 gttgccgttg acgacgctcg cgccgacgag ctcgatgtcc ggcgacaatg cggcggtcat
      481 gagggcgacc gcgtcgtccg tcccggtgtc gacgtcgagg atgatcttgg tggccatgcc
      541 ccccagtgtg cccgctgggc ggcggcgacg cgatgagtgc gagcagcatc gtgaggcgta
      601 gactccgcgg acatgatcct gtccacgtca cccagggccg cgtcgatcgc atcgtggatg
      661 cgcgggctgg tgttggtgtt cgctgccctg ttcgcgagcc atgacgccgt ctacctcgtc
      721 cggttcgggc cgggagcggg gtacgcgaac gcgatgtcag ccggtggcca cgatggttac
      781 tggctgcccg cgagtctcgg cctggtcatc ggcgcctcga tcgcattcct gagcgtcgtc
      841 cacgtcatca tccgactccg ccgggatatc ggcgctccgc cgcacgccgc ggccgtcaca
      901 cccggcggtc cgtcttatgg cgccgagctc gcgcggctgt ggctgcgcct gctggttgcg
      961 gtcggcctcc tgtttgctgt ccaggagaac atcgagggcc tgctggccaa cggtggcatc
     1021 gccggtctcg acccgctgtt cggccacgac gcctggctgg tgccgccgat cctcgctgcg
     1081 acaaccttcc tgctcgccgc agtcggggcg gtcctccgct ggcgggtccg catcctgcag
     1141 gcgcgggtcg aagctgcccg gcgcgcccgg gccttcggcc gcccgatcgc ctcggcgccg
     1201 tctcggcgct ggctcacgat gcccgcgggt ggcgggcatg gcccggatgc cgcccggccg
     1261 gacgccgggc gtgccccgcc gatcgtgatc gcggcggccg cctaagcggc gtccgcctcc
     1321 cgagtcgtgc ggccgccgtc cccggccgcc agcctacgtc ccgcgccggt tcgccggctg
     1381 gtgggacggt cggaggaacg atcacgtgaa tcgcacccga acggtgcggc gcctcggcgg
     1441 cgccgtcact gtcgcagccc tgttcagcct gcttgccgcc ggcacctcgc tcgcacacga
     1501 ggtgcgggat gtcggggagt acaccgtcgt ggtcgggttc atcgacgagc ccgtcttcgt
     1561 cggccagaag tccggtctcg agttcttcgt ggacaagggc gacacgcccg tcgacggcct
     1621 ggagcagacg ctcaaggccg aggtgatcta ccaggaccag caccgcgacc tgccgatcga
     1681 ggcgcgcttc ggcgaggagg gcgcgtatga gtcggtgttc ttcccgaccg ccgccgggcc
     1741 ctacaccttc cacatctcgg ggaccctcga cggtcaggcg attgacgagt ccttcacgtc
     1801 cagcccggag ggattcagcg aggtggagga cctggcgtcg gggcagttcc cggtccagtt
     1861 cccgtcgcag gccgaagtgg tgagcgacgc tcaagcgggc cgagccgccc agacgcaggc
     1921 gacgatcggt atcgcggtcg gtgtcattgg cctggtcgtc gcggtcgtcg cggtcggcct
     1981 cgcgctgtcc gtccgccgcc gagccccagc tccgaagtga ccgtccgacc ggccatccga
     2041 cggttggccg gcgcgctcct cctggcgggc ccgatcctga tcgggcccgc cgctccggtc
     2101 gcgctggccc atgccgagct cgtgaccagc gcgcccgcgg ccggtgacgt cgtcgcgacc
     2161 gcgcctgcgg agatccgcct ggtcttctcg gagacgatcg acggcgcgta cacgcggctc
     2221 gacctcctcg acccgctcgg ccacatgatc gcggacggga tcggggcccc cgacccatca
     2281 gacccgtaca cactggtcgc gccgatcgag ccgctcccag acgggctgta tacggtgaac
     2341 tggcaggcgc tctcgcgcgc cgatggccat cgcaccagtg gattcttcac gttcggcgtc
     2401 ggcgacgttg cgccaccagc gggtggcggc caggacgccg gggggagcat ccacgccggc
     2461 cacgatgcgg tgacggccct gatcgagacg gaatcgcggt tcgcgcagct gctcgggttg
     2521 ctgctcgcgg tcggcctgcc ggtgttcgtg ttcaccgccc tgaagatcga gccgtcacgg
     2581 cggctcggcc aggccggcgc cggggcgctg ctcatcgctg ccgtcggcgc cttcgggctg
     2641 ctggcggtga acgccgccgg cttcggcacc gacccggtcg cgtacgcgcg cgacacgcgc
     2701 agcggccagt tgctcgcggc tggggcgctc ctggccctgg ccggtgggct ggtcgtgctc
     2761 ggactcgccc gacgtcgctc gcc
//
LOCUS       DBNHXO010000079         2599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4782502, whole genome shotgun sequence.
ACCESSION   DBNHXO010000079 DBNHXO010000000
VERSION     DBNHXO010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4782502"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="AC162A_01710"
     CDS             complement(<1..738)
                     /locus_tag="AC162A_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIR5009580.1"
                     /translation="MPYGPHVADDRARMLETIGVASVDDLFEDIPPALRASPLRIDPP
                     EPELDLSRRLSTLAARNRVDLASFLGAGAYRHWTPPAVDQLLLRGEWYTAYTPYQPEV
                     SQGTLQSIYEYQSLLAELTGLDVVSASHYDGGAATAEAALMTCRATRRDRILISRAVH
                     PHYRQTLQTYAEGAGLALDEVPLVASGALAGTTDLEALARLLAEPDRPVAGVVAAQPG
                     ILGLLEDMAEIGRLAHAAGALFVSVIEP"
     gene            complement(742..1122)
                     /gene="gcvH"
                     /locus_tag="AC162A_01715"
     CDS             complement(742..1122)
                     /gene="gcvH"
                     /locus_tag="AC162A_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR5009581.1"
                     /translation="MVPDDLRFTKEHEWVRVEGDEAIVGITQYAADQLGDIVFVELPA
                     VGKVLEQYATFGVVESVKAVSDLFAPVAGEVVGVNDALADKPELVNGEPFGDGWMVRV
                     RLADAAQVEGLLDAAGYERIVAEG"
     gene            complement(1162..2295)
                     /gene="gcvT"
                     /locus_tag="AC162A_01720"
     CDS             complement(1162..2295)
                     /gene="gcvT"
                     /locus_tag="AC162A_01720"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR5009582.1"
                     /translation="MTEATTTTPLPSPLLDRHERLGAKIVEFAGWLMPIQYAGILEEH
                     RAVRSAAGLFDLSHMGELVVEGPDAGAALAAALVTDPPALAVGRAQYSMICADDGGIM
                     DDLIVYRLAEDRFMVVANASNARVVSDALAERMEGFQAVLDDRSLATGLVAIQGPRSI
                     DILGPLTSLDIGAIRYYGIAEGEVAGMPVQVARTGYTGEDGFELFADVDRVGELWDTL
                     LEAGRPHGQVPVGLGARDTLRLEAGMPLYGNELDASTNPFEAGLGRVVKLSKPGDFVG
                     RAALERVVRDGVGRQLIGLEMRDRGIARHGYPVLAPASADAPAGPVGSVTSGTMSPTL
                     GTAIAMAYARPVDAGPGTMLAVEIRGVPVAAEVVPLPFYKRPA"
     gene            complement(2292..>2599)
                     /locus_tag="AC162A_01725"
     CDS             complement(2292..>2599)
                     /locus_tag="AC162A_01725"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5009583.1"
                     /translation="VREVREETGLEVRIISPFDHIDYTFVMGGTRISKTVHYFLMVST
                     GGDLARHDHEFAEVRWVSFDEAATLLTFDTERSLVSRAAEVAVGGSFPLPALGERTA"
BASE COUNT          380 a          956 c          917 g          346 t
ORIGIN      
        1 gggctcgatc acgctcacga acagcgcgcc cgcggcatgc gcgagccgcc cgatctcggc
       61 catgtcctcg agcaggccca ggatcccggg ctgcgccgcg accacgccgg ccacgggacg
      121 gtccggctcg gcaagcagcc gcgccagggc ctcgagatcg gtggtcccgg ccagcgcgcc
      181 ggacgcgacc agcgggacct cgtcaagcgc gagcccggcg ccctcggcat aggtctggag
      241 cgtctggcgg tagtgcgggt gcaccgcccg tgagatcagg atccgatcgc ggcgggtggc
      301 gcgacaggtc atcagggccg cctctgccgt cgccgcgccg ccgtcgtagt gggaggcgga
      361 gacgacgtcg aggccggtca gctccgcgag caatgactgg tactcgtaga tgctctggag
      421 cgtcccctgg ctgacctcgg gctgatacgg cgtgtacgcc gtgtaccact cgccacggag
      481 cagcagctgg tcgacggccg gcggtgtcca gtggcggtac gcgccggcac cgagaaagct
      541 cgcgaggtcg acacggttgc gggccgcgag cgtgctcagt cgccggctca ggtccagctc
      601 gggctccggg gggtcgatcc gcaaggggct cgcccgcagg gcaggcggaa tgtcctcgaa
      661 caggtcgtcg accgacgcca cgccgatcgt ctcgagcatc cgggcccgat cgtcggcgac
      721 gtgcggcccg tagggcatcg gtcagccctc ggcgacgatg cgctcgtacc cggcggcgtc
      781 gaggagcccc tcgacctgcg ccgcgtccgc gagccgaacc cggaccatcc agccgtcgcc
      841 gaacggctcg ccgttcacga gctccggctt gtccgcgagc gcgtcgttga ccccgacgac
      901 ctcgcccgcg accggcgcga acaggtcgct gacggccttc acggactcga ccacgccgaa
      961 cgtcgcgtac tgctcgagca ccttgccgac cgccggcagc tcgacgaaca cgatgtcgcc
     1021 gagctggtcc gccgcgtact gggtgatgcc gacgatcgcc tcgtcaccct ccacgcggac
     1081 ccactcgtgc tccttggtaa agcgcaggtc gtcggggacc atcgggcggg ctcctccgtt
     1141 cggcgtcgag tgggatccgg ctcaggctgg gcgcttgtag aacgggagcg gcacgacctc
     1201 cgccgcgacg ggcaccccgc ggatctcaac cgccagcatc gtaccgggtc cggcatcgac
     1261 cggacgggcg taggccatcg cgatcgccgt gccgagcgtg ggtgacatgg tgcccgaggt
     1321 caccgagccg accgggcccg ccggcgcgtc ggccgaggcg ggcgcaagca ccgggtagcc
     1381 atgccgggcg atcccgcggt cccgcatctc cagcccgatc agctggcggc cgacgccgtc
     1441 gcgcacgacc cgctcgagcg ccgcgcgccc cacgaagtcg ccgggcttgg acagcttcac
     1501 gacccgtccg aggcccgcct cgaacggatt ggtcgaggcg tcgagctcgt tgccgtagag
     1561 cggcatgccc gcctcgaggc gcagggtgtc gcgggcgccg aggccgacgg ggacctggcc
     1621 gtgcggccgt cccgcctcga gcagcgtgtc ccacagctcc cctacccggt ccacgtcggc
     1681 gaacagctcg aagccgtcct cgcccgtata gccggtccgt gcgacctgga cgggcatgcc
     1741 cgccacctcg ccttcggcga tcccgtagta ccggatcgcg ccgatgtcga gcgaggtcag
     1801 cgggcccagg atgtcgatcg accgggggcc ctggatggcc accagcccgg tcgcgagcga
     1861 gcgatcatcc aggacggcct ggaacccttc catgcgctcg gcgagcgcgt ccgagacgac
     1921 cctcgcgttc gaggcgttgg cgacgaccat gaaccggtcc tcggccagcc ggtacacgat
     1981 caggtcgtcc atgatcccgc cgtcgtcggc gcagatcatc gagtactgcg cgcgcccgac
     2041 cgcgagcgcc gggggatccg tcaccagggc cgcggccagc gcggcgccgg cgtccggccc
     2101 ttcgacgacc agctcgccca tgtgcgacag gtcgaacagc ccggccgccg agcggaccgc
     2161 gcggtgctcc tcgaggatcc cggcgtactg gatcggcatc agccagccgg cgaactcgac
     2221 gatcttcgcg cccaaccgct cgtggcggtc gaggagcggg ctgggcagcg gcgtcgtcgt
     2281 cgtggcctcg gtcatgcggt gcgctccccc agggccggca gcgggaacga cccaccgacg
     2341 gcgacctcgg ccgcccgcga gacgagcgac cgctcggtgt cgaacgtcag cagcgtagcg
     2401 gcctcgtcga atgagaccca gcggacctcc gcgaactcgt gatcgtggcg cgcgaggtcg
     2461 ccgccggtcg aaaccatcag gaagtagtgg acggtcttcg agatgcgcgt cccacccatc
     2521 acgaacgtgt agtcgatgtg gtcgaacggg ctgatgatcc ggacctcgag acccgtctcc
     2581 tcgcggacct cgcgcacgg
//
LOCUS       DBNHXO010000080         4787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2004629, whole genome shotgun sequence.
ACCESSION   DBNHXO010000080 DBNHXO010000000
VERSION     DBNHXO010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2004629"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="AC162A_01730"
     CDS             <1..866
                     /locus_tag="AC162A_01730"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016297 - fatty acyl-[ACP] hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HIR5009584.1"
                     /translation="VARSPGRAEARVSAAGAEEDDPKAANRTATVAYRVRFDECGPDG
                     LARTSTMLRYAQDVAWIHSERLGFDRAWYADRGLTWLVRAAQVTILRPVPLGTTLDVA
                     TTVSGFRKVWARRRTDGRLADGTLVFWAHTDWVIVDARGLPTRVPAEFPEVYGTMPGP
                     FEPGRVALPPTPPGASAHRGLVRPQDLDPLGHVNNAAYLDYLEEALLAAGDEAAAAVT
                     ASPRSVWLEYLVQAIPGAIVDGSSWPLAGGPGEDATRAWRLTDEAGIELARGRHAVGA
                     AAIKVSAGSGA"
     gene            863..2065
                     /locus_tag="AC162A_01735"
     CDS             863..2065
                     /locus_tag="AC162A_01735"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004561244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase family protein"
                     /protein_id="HIR5009585.1"
                     /translation="MTRMVFRGGSVFDGTGSDPAPGDVAIEDGRIVDVGTGLDGDESI
                     DITGKTLLPGLFDCHVHMIFSHVDTWRQVNTPFSYRFYEAADNLRATLRTGITSVRDA
                     GGADLGIQQALADGLIEGPRMQISLTMLSQTGGHGDDWLVSGVRVTLMPAYPGVPSAI
                     VDGPDEMRRKVRELHLMGANVVKVATSGGVLSARDNPAHAHFSPEELDVLVAEATAAG
                     MFVMAHAQGADGIKNAVRAGIRSIEHGIFLDAEGIDLMLGHGTWLVPTLVAPQGVIDA
                     AANGVVFPPGVLEKANSLADLHRRSVGTAIEAGVRIAMGTDSGVTPHGQNLRELSMMA
                     GLGMKPAAVLEASTRSAAQLMGVDDELGTVESGKAADLVLVDGDPYEFDTLADRIDGV
                     WLRGVRVV"
     gene            2283..3872
                     /locus_tag="AC162A_01740"
     CDS             2283..3872
                     /locus_tag="AC162A_01740"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphohydrolase"
                     /protein_id="HIR5009586.1"
                     /translation="MDVQIAAAAVLLVAALIGALAGSAGYSLVVERHQTRRVARLAER
                     LAHAADEGALVALEAERIADPRLRAAFANLADRVADTWSLATVDPLTGIANRQTVLAR
                     LDEELTRANRYRRQLSVIIIDLDHFKRLNDAHGHAAGDSVLRHVGQRLAASVRTVDVA
                     GRYGGEEFLVVLPETGPDAAASLAEKLRRAVGGSEVRLPDGEMVSVTLSAGVAGGQGE
                     LLNLDALVRDADAALYSAKALGRDQVYVFRETGDEGTVRRAPISSEARTRALDVGRSA
                     MDAAQDALITALAGREAWSGKPSTLIAEASVALGRAVDLPSGELDRIRTASLLHDLGK
                     LAIPEDILARPGDLAISEWRVVTEHPKIGQVVLEQAGALRDAATIVLHHHEWYDGRGY
                     PHGLNGSDIPVGARIVSIVDAYEAMIAGRPYRAAISHRAALAELRRQAGIQFDPDLVE
                     VFAALFADGLPWDLDGHAVIPGHARVETAETEVVAAGTASGRGSKGGGRRSAAVAPGG
                     PRRRSTAEAHDAVHDKRRRAG"
     gene            3941..>4787
                     /locus_tag="AC162A_01745"
     CDS             3941..>4787
                     /locus_tag="AC162A_01745"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5009587.1"
                     /translation="MDRGRPTRDVALLFATRSLRMFGYGFLAVVLVLYLESLGLDGAS
                     IGLLLGLTLLGDAAISLLLTTRADRFGRRRTLFLGSLLMVGAGIAFASTDLFPVLLVA
                     ATLGVLSPSGNEVGPFLAIEQASLAQLVDDRSRTSLFARYQVVGSVATAFGSLGAGIV
                     TQAALQAGFVQVDAYRVVIVGYAVIGVAMAFVFPLLSQAVEVPARSAAPGEVVSAAQP
                     ATRLGLHRSRGIVARLSALFALDAFGGGFVMQGFIAFWLTSQFGADPAQIGAILFGAN
                     ILAAAS"
BASE COUNT          617 a         1715 c         1767 g          688 t
ORIGIN      
        1 gagtcgctcg atcgcctggc cgggcggaag cccgcgtgag cgcggcaggc gcggaggagg
       61 acgaccccaa ggcggccaac cggacggcca cggtcgcgta tcgcgtccgc ttcgacgagt
      121 gcgggcccga cggcctggcc cggacgtcga cgatgctgcg atacgcccag gacgtggcct
      181 ggatccactc cgagcgcctc gggttcgacc gggcgtggta cgccgatcgc ggcctcacct
      241 ggctggtgcg ggcggcgcag gtgacgatcc ttcgaccggt gcccctgggc acgacgctgg
      301 acgttgcgac gacggtgtcc gggttccgca aggtatgggc gcgtcgccgc accgacgggc
      361 ggctcgcgga cggcaccctc gtgttctggg cgcacaccga ctgggtgatc gtcgatgccc
      421 gcggcctgcc gacgcgcgtt cctgcggagt tccccgaggt ctacggcacc atgcccggcc
      481 cgttcgagcc cgggcgagtc gcgctgccgc cgacgccacc tggagcctcg gcgcatcgcg
      541 ggctcgtccg gccacaggat ctcgacccgc tgggtcacgt caacaacgcc gcctacctcg
      601 actacctcga ggaggcgctg ctcgccgccg gcgacgaggc cgccgccgcc gtcacggcgt
      661 cgccgcggtc cgtgtggctc gaatatcttg tccaggccat cccgggggcg atcgtcgacg
      721 ggtcgagctg gccgcttgca ggcgggccgg gcgaggacgc gacgagggcc tggcggctga
      781 cggacgaggc ggggatcgag ctcgcgcggg ggcggcacgc ggtcggggca gccgcgatca
      841 aggtgagtgc agggagcggc gcatgacgcg gatggtcttt cgcgggggct cggtcttcga
      901 cggcaccggg tcggatccgg cgcccggcga cgtcgccatc gaggatggcc gcatcgtgga
      961 cgtcgggacc gggctcgatg gcgacgagtc catcgacatc accggcaaga ccttgctgcc
     1021 cggcctgttc gactgccacg tgcacatgat cttcagccac gtcgacacct ggcggcaggt
     1081 gaacacgccg ttctcgtacc ggttctacga ggcggccgac aacctccggg ccaccctccg
     1141 gaccgggatt acgtccgtcc gggatgcggg cggggcggac ctgggcatcc agcaggcgct
     1201 cgccgacggc ctcatcgaag ggccgcggat gcagatcagc ctgacgatgc tcagccagac
     1261 cggcggtcac ggcgacgact ggctcgtgtc gggcgtacgc gtcacgctga tgccggccta
     1321 tcccggcgtg ccctcggcca tcgtcgacgg tcccgacgag atgcggcgca aggtgcgcga
     1381 gctgcacctg atgggcgcga acgtggtcaa ggtcgcgaca tcgggcggcg tgctctcggc
     1441 gcgcgacaac ccggcccacg cgcacttcag ccccgaagag ctggacgtgc tcgtggctga
     1501 ggcgacggcc gcggggatgt tcgtgatggc gcacgcccag ggggccgacg ggatcaagaa
     1561 cgccgtccgc gccggcatcc gctcgatcga gcacggcatc ttcctcgatg ccgaggggat
     1621 cgacctgatg ctggggcacg gcacgtggct cgtgccgacg ctggtcgccc cacagggcgt
     1681 gatcgacgcg gccgccaacg gcgtcgtgtt cccgccgggc gtgctcgaga aggcgaactc
     1741 cctcgccgac ctccaccggc gaagcgtcgg gacggcgatc gaggcgggcg tgcggatcgc
     1801 gatgggcacc gactccggcg tcacgccgca tggccagaac ctgcgcgagc tgtcgatgat
     1861 ggccgggctg gggatgaagc cggccgcggt gctcgaggcg agcacccgca gcgcggcgca
     1921 gctgatgggc gtggacgacg agctcgggac ggtcgagtcc ggcaaggccg cggacctcgt
     1981 gctcgtcgac ggcgacccgt acgagttcga caccctggcc gaccggatcg acggagtctg
     2041 gctgcgcggg gttcgtgtcg tctgaggcct cgtccgcctg cccggcggcc gcccgtccgc
     2101 ctataggact tttgggctat gacccaccga ccccccgggg ttgcgcgcgg gaaccggggg
     2161 tgcgccccgc atcggggacg ggaccatctc gccatggagt tcgggccatc cccacgtagc
     2221 ctgttggtac ggggcgccga acggtggcgc atgccgccct ggcgaaggtc gcgagaggtc
     2281 cgttggacgt ccagattgcc gcagcagccg tgctgctcgt cgccgcactg atcggggcgc
     2341 tcgccggctc ggcagggtac tcgctggtcg tcgagcgcca ccagacccgg cgcgtcgcgc
     2401 ggctcgcgga gcggctcgcc catgcggccg acgagggtgc gctcgtcgca ctcgaggcgg
     2461 agcgcatcgc cgacccgagg cttcgggcag cgttcgccaa cctcgcagac cgcgtggccg
     2521 acacctggag cctcgccacc gtcgatccgc tgaccgggat cgccaatcgc cagaccgtgc
     2581 tcgcgcggct cgacgaggag ctcacgcgcg ccaaccggta tcgacgccag ctgtcggtga
     2641 tcatcatcga cctcgaccac ttcaagcgcc tcaatgacgc ccatggccac gcggccgggg
     2701 actcggtcct gcggcacgtc ggacagcgtc tcgcggcgag cgtccggacc gtggacgtcg
     2761 cgggccgcta cggcggcgag gaattcctcg tcgtcctgcc cgagacgggc cccgacgccg
     2821 cggcgtcgct ggccgagaag ctgaggcgag ccgtcggtgg cagcgaggtc cggctgccgg
     2881 acggcgagat ggtcagcgtc acgctgtcgg cgggcgtcgc cggcggccag ggcgagctgc
     2941 tcaaccttga cgcgctggtg cgcgacgccg acgcggcgct gtactccgcg aaggccctcg
     3001 ggcgcgacca ggtctacgtg ttccgcgaga ccggtgacga gggcaccgtc cggcgcgccc
     3061 cgatcagctc cgaggcacgc acgcgggcgc tcgacgtcgg gcgctccgcg atggacgcgg
     3121 cgcaggatgc cctgatcacg gccctcgccg gccgcgaagc gtggtcgggc aagccgtcga
     3181 cgctgatcgc cgaggcctcc gtcgccctcg gccgcgcggt cgacctgccg agcggcgagc
     3241 tcgaccgcat ccgcaccgcg agcctgcttc acgacctcgg caagctcgcg atcccggagg
     3301 acatcctcgc ccggcccggc gacctcgcga tctccgagtg gcgcgtcgtg accgagcatc
     3361 ccaagatcgg ccaggtcgtc ctcgagcagg ccggcgcgct ccgggacgcg gcaaccatcg
     3421 tgctccacca ccacgagtgg tacgacggcc gcggctaccc ccatgggctc aacgggagcg
     3481 acatcccggt gggagcccgg atcgtctcga tcgtcgacgc ctatgaggcg atgatcgcgg
     3541 gccgcccgta tcgcgccgcg atctcgcacc gcgcggcgct cgccgagctc cggcgccagg
     3601 ccggcatcca gttcgacccg gacctcgtcg aggtgttcgc cgcgctgttc gccgacgggc
     3661 tgccgtggga cctcgacggc cacgccgtga tccccgggca cgctcgggtc gagaccgccg
     3721 agacggaggt cgtcgcggcc ggcaccgcaa gcggtcgcgg atcgaagggc ggcgggcgcc
     3781 ggtccgctgc cgttgcgccc ggaggacccc gtcgccgttc gaccgccgag gcgcacgacg
     3841 cggtccacga caagcgccgg cgcgccggct agcaacgccg cgggcctcgc acggcggagg
     3901 ccccgtacgg acggccagcc cgtccggcag gattggcgtc gtggatcgag gtcgaccgac
     3961 ccgggacgtc gcgctgctgt tcgcgacgcg cagcctgcgc atgttcgggt acgggttcct
     4021 cgccgttgtc ctcgtcctgt acctggagtc gctcgggctc gacggcgcat ccatcgggct
     4081 gctgctcggc ctgacgctgc tgggcgacgc ggcgatctcg ctgctgctga ccacccgcgc
     4141 cgaccggttc ggtcgccggc ggacgctgtt cctgggctcg ctgctcatgg tcggcgccgg
     4201 catcgccttc gcgtcgaccg acctgttccc cgtgctcctc gtcgcggcga ccctgggcgt
     4261 gctcagcccg agcggcaacg aggtcgggcc gttcctcgcg atcgagcagg cgtccctggc
     4321 ccagctggtc gacgatcgca gccggacgtc gctgttcgcc cggtaccagg tggtgggctc
     4381 ggtcgccacg gcgttcgggt ccctgggcgc gggcatcgtg acgcaggccg ccctgcaggc
     4441 gggcttcgtc caggtggacg cgtatcgggt ggtgatcgtc ggttacgcgg tgatcggcgt
     4501 cgcgatggcg ttcgtgttcc cgctgttgtc acaggcggtc gaggtcccgg cccggagcgc
     4561 cgcaccgggt gaggtcgtgt cggccgcgca gcccgcgacg cggctcggac tgcatcgatc
     4621 tcgcgggatc gtcgcccggc tgtcggccct gttcgcgctt gacgcgttcg gcggtggctt
     4681 cgtcatgcag ggcttcatcg cgttctggct gacgtcccag ttcggcgccg acccggcgca
     4741 gatcggcgcg atcctgttcg gggccaacat cctcgccgcg gcgtccg
//
LOCUS       DBNHXO010000081         2496 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2204080, whole genome shotgun sequence.
ACCESSION   DBNHXO010000081 DBNHXO010000000
VERSION     DBNHXO010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2204080"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="AC162A_01750"
     CDS             complement(<1..930)
                     /locus_tag="AC162A_01750"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR5009588.1"
                     /translation="MTTAVDLAALARDAVPAEVVARLRGAREVLAVGHENPDADTLGA
                     TLAVCRLVESLGGRATAMFSDVPPPLYAFIPDVERVRTDPDPSVAYDLLVLSDCATAE
                     RVGPVADRHPALFADLSRLVIDHHASNDAQGPGDWVDPGAAATCEMVALLAVRLGIPL
                     DAGGGALANALMAGIVMDTATFAHPNATPRTLAVSAALVEAGAPLSDISRRLYRTKPD
                     AQLRLFGRVLERLRTAPDGRVIWSTLLGGDLEATGALPAHSEGIIDLLAQSETAVVAM
                     LLKEQDDGTTRLSVRTKPGGVDATVLTGLFGGGG"
     gene            complement(927..1505)
                     /gene="rbfA"
                     /locus_tag="AC162A_01755"
     CDS             complement(927..1505)
                     /gene="rbfA"
                     /locus_tag="AC162A_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR5009589.1"
                     /translation="MSQRIDRVDELLRQEIGALLAKEVADPRIGFATITDVETSPDLG
                     HAKVWVSVIGGKADRDETLRALQSSMGYIRHELGRRLRIRRIPELHVRLDDSAERGTR
                     LLHLLNELEAGTDPQAIVPFEESLPKPVKRLPHEGDTDEPVAQPPAKPTRKRPSARRG
                     SYPARASSHRAKGGDKPAGGKPRSGKSKPPRG"
     gene            complement(1519..>2496)
                     /locus_tag="AC162A_01760"
     CDS             complement(1519..>2496)
                     /locus_tag="AC162A_01760"
                     /inference="COORDINATES: protein motif:HMM:NF023413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR5009590.1"
                     /translation="VGTVIEAELDKGRGPVATLIVQTGTLRVGNVVVVGNTFGKVRAL
                     ETAEGKRVQKVGPSSAVVILGLADVPEAGDILRVVGDEKIARTQVETRKNEQAAREGG
                     GGGRATLEDLYRQIQAGQAKELRIILKADVSGSLGAIDHALEQLGTDEVKINILLEAA
                     GDITDNDIMLATASNAIVVGFNTAITETARRAAESEGVDVRLYDIIYKLTDDIDAALK
                     GLLEPEIVEVVEGRAEVRQIFRVGKSTVIAGCYVTDGRIVRNGGARVWRGGKVIETDK
                     IDSLRRFRDDVREVAQNFECGIGLAGYNDLVEGDIIECFTNQTRSRVVG"
BASE COUNT          321 a          899 c          897 g          379 t
ORIGIN      
        1 gcccccgccg ccgaacaggc cggtcaggac ggtggcatcg acgccaccgg gcttggtccg
       61 cacgctgagg cgcgtggtgc cgtcgtcctg ctccttgagc agcatcgcga ccacggcggt
      121 ctcggactgg gcgagcaggt cgatgatccc ctcggagtgc gcgggcagcg cgccggtcgc
      181 ctcgaggtcg ccgccgagca gcgtcgacca gatcacgcgg ccgtcgggcg cggtccgcag
      241 ccgctccagc acccgaccga acaggcgcag ctgggcgtcg ggcttggtcc ggtacagccg
      301 ccgcgagatg tcggacagcg gcgcgcccgc ctcgacgagc gccgccgaga ccgccagcgt
      361 cctcggcgtc gcgttcggat gggcgaaggt cgcggtgtcc atgacgatgc cggccatcag
      421 cgcgttcgcg agcgcgccgc cgcccgcgtc gagggggatg cccagccgca ccgcgagcag
      481 ggcgaccatc tcgcaggtcg ccgcggcacc cggatcgacc cagtcccccg gtccttgtgc
      541 gtcgttcgac gcgtggtggt cgatcacgag gcgcgacagg tccgcgaaca gggccggatg
      601 gcggtcggcc acgggcccga cgcgctccgc ggtcgcgcag tccgacagca ccagcaggtc
      661 gtaggcgacg gacgggtcgg ggtcggtgcg cacgcgctcg acgtcgggga tgaacgcgta
      721 gagcggtggc ggcacgtcgg agaacatggc cgtcgcccgg ccccccagcg actcgaccag
      781 gcggcacacg gccaaggtcg cccccagggt gtcggcgtcg gggttctcgt ggcccacggc
      841 gagtacctcg cgggcaccgc gcagccgcgc gaccacctcg gcggggacgg cgtcacgggc
      901 gagggcggcg aggtcgaccg cagtcgtcat ccgcggggcg gtttcgactt gccggaccgg
      961 ggcttcccgc cggccggctt gtcgccgccc ttcgcccgat ggctgctggc ccgcgcggga
     1021 tacgagccgc gtcgggcgga tggccgcttc cgggtgggct tcgcgggcgg ctgggccacg
     1081 ggttcgtccg tgtcgccctc gtgcggcagg cgcttcaccg gcttcgggag cgactcctcg
     1141 aacgggacga tcgcctgcgg gtccgtgccg gcctcgagct cgttgagcag gtgcagcagg
     1201 cgggtgccac gctcggccga atcgtcgagg cgcacgtgga gctcggggat gcgccggatc
     1261 cggagccggc gtccgagctc gtggcggatg tagcccatcg aggattggag cgcgcgcagc
     1321 gtctcgtcgc gatcggcctt gccgccgatc acgctcaccc agaccttggc atgaccgagg
     1381 tcgggcgacg tctcgacgtc cgtgatcgtc gcgaacccga tccgcgggtc ggcgacctcc
     1441 ttcgcgagca gcgccccgat ctcctggcgc agcagctcgt cgacccggtc gatcctctgg
     1501 ctcatgggcg cgacgccgtc agccgactac ccggctgcgg gtctggttcg tgaagcactc
     1561 gatgatgtcg ccctcgacca ggtcgttgta cccggcgagg ccgatcccgc actcgaagtt
     1621 ctgggcgacc tcacgaacgt cgtcgcggaa ccgccgcagg gaatcgatct tgtcggtctc
     1681 gatcaccttg ccgccacgcc agacccgcgc gccgccgttg cggacgatcc gcccgtcggt
     1741 cacgtagcag ccggcgatca cggtgctctt gccgacccgg aagatctggc gcacctcggc
     1801 gcggccctcg acgacctcca cgatctccgg ctcgagcagg cccttgaggg ccgcgtcgat
     1861 gtcgtcggtg agcttgtaga tgatgtcgta gaggcggacg tcgacgccct cgctctcggc
     1921 ggcgcgccgc gccgtctccg tgatcgcggt gttgaacccg accacgatgg cgttcgacgc
     1981 cgtcgcgagc atgatgtcgt tgtccgtgat gtcgcccgcg gcctcgagca ggatgttgat
     2041 cttgacctcg tccgtgccga gctgctcgag cgcgtggtcg atggcaccca gcgaacccga
     2101 cacgtcggcc ttgaggatga tccgcagctc cttggcctgg ccggcctgga tctggcggta
     2161 gaggtcctcg agcgtcgcgc gaccgccgcc gccaccctcg cgagcggcct gctcgttctt
     2221 gcgggtttcg acctgcgtac gggcgatctt ctcgtcgccc acgacgcgca ggatgtcgcc
     2281 ggcctcgggg acgtccgcca ggccgaggat gaccacggct gacgacggac ccaccttctg
     2341 gacgcgcttg ccttcggccg tctcgagcgc gcggaccttg ccgaacgtgt tgcccaccac
     2401 gacgacgttg ccgacgcgca gcgtgccggt ctggacgatc agcgtcgcca ccggaccgcg
     2461 gcccttgtcg agctccgcct cgatcacggt gccgac
//
LOCUS       DBNHXO010000082         3547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1806579, whole genome shotgun sequence.
ACCESSION   DBNHXO010000082 DBNHXO010000000
VERSION     DBNHXO010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1806579"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="AC162A_01765"
     CDS             complement(<1..706)
                     /locus_tag="AC162A_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HIR5009591.1"
                     /translation="MTRVVLDDDGLDVLELALGGWLPPEAVAGAPGSPEIILTDAENT
                     PLARVTSGEGGSPRIEPLAELARGAGPHWEPDVRLSFAAVRAGVTAGPDRATVVFIVD
                     VPPTRHATESMLLRAAIPEVATVVVVAAVRRHRSPGLVGAAGLTRAAWGLAGTLATAR
                     PEQAIVRLALPWPIGARRDLAPAFADLGASLVEAASELRGPASPGAADVEADYPPASA
                     REVLRASESDAVRPGAV"
     gene            complement(703..1689)
                     /locus_tag="AC162A_01770"
     CDS             complement(703..1689)
                     /locus_tag="AC162A_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR5009592.1"
                     /translation="MTLDVPLAIGGLFVGIIVGLTGMGGGALMTPMLVFFFQVDPLTA
                     ISSDIVVSLFMKPVGAFVHLRHGTVNLKLVGWLCLGSVPLAFMGAWLISLVPETVPLD
                     VLLKRALGIALLIATGGLLLRAVMQARYNRLPLGEGPAPMTRPDVVIRPIPTVVLGAV
                     AGLMVGLTSVGAGSIVVVVLLFLYPRLKASSLVGTDLTQAVPLVAAAALGHILFGSFS
                     LAIASSLLVGAIPGTWIGARISSRAPGGVIRRALAILLLASSLKLLGVPDLLVTASAL
                     VALLFAFFGWRLVRDRLKRMPAAERTPLPIPDLGSVTGPGEELVPERDTVTP"
     gene            1852..2715
                     /locus_tag="AC162A_01775"
     CDS             1852..2715
                     /locus_tag="AC162A_01775"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HIR5009593.1"
                     /translation="MITHAPQDLSALDDARLAAALAAGAGEVLLGLRLQADADHPDGD
                     SPDIAAADLRRAGDAAAQAWLAAALAAARPGDAILSEEAADDPVRLTAERVWIIDPLD
                     GTREFGEHRPDGRWRDDFAVHIALWRRARGLTDGAVALPACGVVHSSGTRIPADVAAA
                     RAVLDGSRPLRIAVSRSRPPEVAARLAERADVELVPMGSTGVKVSRVLDGTVDAYVHA
                     GGQHEWDSAAPVAVALASGFVATRLDGSPLVYNQPSPWSPDLVVCHPALADHLRRLLG
                     AAGVEPAGGPE"
     gene            2727..>3547
                     /gene="cysD"
                     /locus_tag="AC162A_01780"
     CDS             2727..>3547
                     /gene="cysD"
                     /locus_tag="AC162A_01780"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HIR5009594.1"
                     /translation="MTQLRTLEAESVHIFREVAAEMQRPCLLFSGGKDSIVMLHIARK
                     AFAPARIPFPVMHVDTGLNFESILAYRDSWIEKLGLQLVIASVEDAIQRGLVQEEPNG
                     SRNRIQTPVLLEAAEKNGFDALFGGGRRDEEKARAKERVFSFRDEFGQWDPKNQRPEI
                     WNLYNGRVFPGQSIRVFPLSNWTELDIWTYIAEEGIEIPELYLAADREVVERAGMLYA
                     VNPFIPLRPGETPQVLRVRYRTVGDANLTAAVVSDADTVEKIVAEIAAVRITERGA"
BASE COUNT          532 a         1313 c         1273 g          429 t
ORIGIN      
        1 agacggcgcc aggcctgacg gcatcggact cactcgcccg gagcacttcg cgcgccgacg
       61 ccggcgggta gtccgcctcg acgtcggcgg cacccggcga ggccgggcct cggagctcgc
      121 tcgcggcctc gaccaggctg gcgccgaggt ccgcgaacgc gggggcgagg tcgcgccggg
      181 caccgatcgg ccacggcagc gcgagccgca cgatcgcctg ttcggggcgg gcggtcgcca
      241 gcgtgccggc caggccccag gcggctcggg tcagcccggc cgcgccgacg agacctggcg
      301 atcggtggcg ccgcacggct gccacgacga cgacggtcgc gacctccggg atggccgccc
      361 gcaacagcat cgactcggtc gcgtggcgcg tgggtggaac gtcgacgatg aacacgaccg
      421 tcgcccggtc cggcccggcg gtgaccccgg cacgtacggc cgcgaacgag agccggacgt
      481 ccggctccca gtgcggcccc gcgccgcgag cgagctcggc cagcggttcg atccgcgggc
      541 taccgccctc gccgctcgtg acccgcgcga ggggcgtgtt ctcggcgtcg gtcaggatga
      601 tctccggcga acccggagcc ccggcgacgg cctcgggtgg aagccagccg ccgagcgcga
      661 gctcgagcac gtcgagacca tcgtcgtcga ggaccacccg ggtcacggcg tgaccgtgtc
      721 gcgctcgggc acgagctcct ctccgggtcc cgtgaccgag ccgaggtcgg gaatcggcaa
      781 cggtgtgcgt tcggcggccg gcattcgctt gagtcgatcg cggaccaggc gccagccgaa
      841 gaacgcgaac aggagggcga ccagcgcgct cgcggtgacc agcaagtcgg gcacgccgag
      901 cagcttgagg ctcgacgcca acagcaggat cgccagcgcg cggcggatca cgcccccggg
      961 ggcccggctc gagatccggg cgccgatcca ggttcccgga atcgcgccca cgagcagcga
     1021 cgacgcgatc gcgagcgaga agctgccgaa caggatgtga ccgagcgcgg cggcggcgac
     1081 gagcggaacc gcctgggtca gatccgtgcc caccagcgaa gaggccttca gcctcgggta
     1141 caggaacagc aggacgacga cgacgatcga tcccgcgccg accgacgtca gcccgaccat
     1201 caggccggcg acggcgccca gcacgaccgt cgggattggc cggatcacca cgtccggacg
     1261 ggtcatcggc gccgggccct cgccgagcgg caggcggttg tagcgcgcct gcatcacagc
     1321 ccgcaggagc aggccgccgg tcgcgatcag cagggcgatg ccgagcgcgc gcttgaggag
     1381 gacgtcgagc gggacggttt cggggacgag cgagatcagc caggcgccca tgaacgcgag
     1441 tggcaccgag cccaggcaca gccagccgac cagcttcagg ttcaccgtcc cgtggcgcag
     1501 gtggacgaag gcgccgaccg gcttcatgaa caggctgacg acgatgtcgc tcgagattgc
     1561 cgtgagcgga tcgacctgga agaagaacac cagcatcggc gtcatcagcg cgccgccacc
     1621 cattcccgtc aggccgacga tgatgccgac gaacagcccg ccgatggcaa gggggacgtc
     1681 cagggtcacg ccgtcgaccg cgccggttgg tgggcggtcg ggatacgaac catgcggcga
     1741 tgataccttc ggacctcacg ggacaacccc accctgcggg ggcccgagga ccgttggccg
     1801 acggccgcgt ccgacccgcc acatccacgc agcgaggccc cttttcgacc ggtgatcacc
     1861 cacgcgccgc aggacctgtc cgccctcgac gacgcccggc tcgccgccgc cctggccgcc
     1921 ggcgccggcg aggtcctcct cggcctccgc ctccaggccg atgccgacca cccggacggc
     1981 gacagccccg acatcgcggc cgccgacctc cgtcgcgcgg gcgatgcggc cgcgcaggcg
     2041 tggcttgccg cggcgctcgc cgcggctcgt cccggcgacg cgatcctgtc cgaggaggcc
     2101 gccgacgacc ccgtccgcct gaccgcggag cgcgtctgga tcatcgaccc cctcgacggg
     2161 acccgcgagt tcggcgagca ccggccggac ggacgctggc gcgacgactt cgccgtgcac
     2221 atcgctctct ggcgccgcgc ccgtggcctg accgacggcg cggtggcgct gccggcgtgc
     2281 ggcgtcgtcc acagctcggg cacgcggatc ccggccgacg tggccgccgc ccgggccgtg
     2341 ctcgacggct cccggcccct ccggatcgcg gtcagccgca gccgtccccc cgaggtcgcc
     2401 gcgcgactgg ccgagcgcgc cgacgtggag ctggtcccca tgggctcaac cggcgtcaag
     2461 gtctcgcggg tgctcgacgg gacggtcgat gcctatgtgc acgccggcgg tcagcacgag
     2521 tgggactcgg cggcgcccgt cgcggtcgcc cttgcgtccg ggttcgtggc gacgcgcctg
     2581 gatggctcgc cactcgtcta caaccagccc agtccgtggt cacccgacct cgtcgtctgc
     2641 cacccggcgc ttgcggacca cctgcgacgg ctgctcggcg cggccggcgt cgagccggcc
     2701 ggggggcccg agtgacgccg cactcgatga cccagctgcg gacgctcgag gccgagagcg
     2761 tgcacatctt ccgtgaggtc gcggccgaga tgcagcggcc gtgcctgctg ttctcgggcg
     2821 gcaaggactc gatcgtgatg ctccacatcg cgcgcaaggc gttcgcgccg gcgcggatcc
     2881 cgttcccggt catgcacgtc gacaccggcc tcaacttcga gtcgatcctc gcctaccgcg
     2941 acagctggat cgagaagctg ggactgcagc tggtcatcgc ctcggtcgag gacgcgatcc
     3001 agcgcgggct cgtccaggag gagccgaacg gctcgcgcaa caggatccag acgccggtgc
     3061 tgctcgaggc ggccgagaag aacggcttcg acgcgctgtt cggcggcgga cggcgcgacg
     3121 aggagaaggc gcgcgcgaag gagcgggtct tctcgttccg cgacgagttc ggccagtggg
     3181 atcccaagaa ccagcggccc gagatctgga acctctacaa cggccgcgtg ttcccgggcc
     3241 agagcatccg cgtgttcccg ctgtcgaact ggaccgagct cgacatctgg acctacatcg
     3301 ccgaggaggg catcgagatc ccggagctct acctcgccgc cgaccgcgag gtcgtcgagc
     3361 gcgccgggat gctgtacgcg gtcaacccct tcatcccgct ccgtcccggc gagacgccgc
     3421 aggtcctccg cgtccgctat cggaccgtcg gggacgcgaa cctcacggcc gccgtggtct
     3481 cggacgccga caccgtcgag aagatcgtgg ccgagatcgc cgccgtccgg atcaccgagc
     3541 gcggcgc
//
LOCUS       DBNHXO010000083         2158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2798733, whole genome shotgun sequence.
ACCESSION   DBNHXO010000083 DBNHXO010000000
VERSION     DBNHXO010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2798733"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="AC162A_01785"
     CDS             complement(<1..502)
                     /locus_tag="AC162A_01785"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR5009595.1"
                     /translation="MREIQAAVIERAGEATTVEALRLDDPRPGEVLVRMTASGVCHSD
                     LHVRDGDWERPGPIVLGHEGAGVIEAVGAGLDPSAVGLAVALSWYAPCLRCRECQRGR
                     QWLCSGSPSLHHTQADGTSRLTRADGSPVLAYLSIGTMATAQVVPATAVVPMPDGVPP
                     EVAALIG"
     gene            complement(549..>2158)
                     /locus_tag="AC162A_01790"
     CDS             complement(549..>2158)
                     /locus_tag="AC162A_01790"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HIR5009596.1"
                     /translation="EDALGRVAWGEVAELRWPLATVGCHLASLEVRQHAAVHAAALRA
                     LRSDAGPDVEVAAGVTVAEVLATFRSIARLQARFGVEACRRYVISFTTSPDDVADVLE
                     LARLAAQTEPFGRPVPALADLPPDLPVLDVVPLLESADALAGATELLDGLLDDPAYRA
                     HLRERGDRQEVMLGYSDSSKESGVLAANWLLYRAQEALIATSRRHGIALTLFHGRGGA
                     LGRGGGPANRAILAQAPGSVGGRLKFTEQGEVIAGHYADVSIAQRHLEQVTAAALLAS
                     TPEHERSTEEAASIGRATMTELTAVSRTAYRSLVDRPAFGAFFRSATPIDLIAGLGLG
                     SRPSARPGSSGRLAAGTAPVAPLDVDALRAIPWVFAWSQARANLPGWYGLGTALEAIT
                     ERGGKETIDHLAGLYRRWPFFASVLDNAELSLAKADLGTFHRYADLAEGPGAIAIRGM
                     IEAEYARSVRMLLLITSRERLLAGHPTLERSIDLRNPYVDALSAVQVELLGRLRRPDL
                     TAADEAAIRNVIGVTINGIAAGLQNTG"
BASE COUNT          295 a          789 c          820 g          254 t
ORIGIN      
        1 agccgatcag cgccgcgacc tcgggcggca cgccgtcggg catcgggacg accgccgtcg
       61 ccggcacgac ctgcgccgtg gccatcgtgc cgatcgagag gtaggcgagg accggcgagc
      121 catccgcccg ggtcaggcgc gacgtgccgt cggcctgcgt gtggtgcagc gacggcgagc
      181 cggagcacag ccactggcgg cctcgctggc actcgcggca gcgcaggcaa ggggcgtacc
      241 acgacagtgc gaccgccagg cctacggcgg acgggtcgag cccggccccg acggcctcga
      301 tgacgcccgc gccttcgtgg ccgagcacga tcggccccgg gcgttcccag tcgccgtcgc
      361 gcacgtggag gtcggagtga cacacgcccg acgcagtcat gcgcacgagc acctcacccg
      421 gccgcgggtc gtcgaggcgc agcgcctcga cggtggtcgc ctcgccggcg cgctcgatga
      481 cggccgcctg gatctcgcgc atgcccctac gatgcccgat cgcgggcggc tccacccggc
      541 ggttcgggct agccggtgtt ctggagcccc gccgcgatgc cgttgatcgt gacgccgatc
      601 acgttcctga tcgccgcctc gtcggccgcc gtgagatccg gccggcgcag gcggccgagg
      661 agctccacct ggacggccga cagcgcgtcg acgtacgggt tgcggaggtc gatcgaccgt
      721 tccagcgtcg ggtgaccggc gagcaggcgt tcgcggctgg tgatgagcag cagcatccgg
      781 actgaccggg cgtactcggc ctcgatcatg ccccggatcg cgatcgcccc cggaccttcc
      841 gcaaggtcgg cgtaccggtg gaacgtgccc aggtcggcct tggccagcga cagctcggcg
      901 ttgtcgagca cggacgcgaa gaacggccag cggcggtaga ggcccgcgag gtgatcgatg
      961 gtctccttgc cgccccgctc ggtgatcgcc tcgagcgccg tcccgaggcc gtaccagccg
     1021 ggcagattgg cccgcgcctg cgaccacgcg aacacccacg ggatcgcccg caacgcgtcg
     1081 acgtcgaggg gcgcgacggg cgcggtaccg gccgccaggc gcccggagga acccggccgc
     1141 gcggagggtc gcgagcccag gcccaggccc gcgatcaggt cgatcggcgt ggccgagcgg
     1201 aagaaggcgc cgaacgccgg ccggtcgacc agcgagcgat aggcggtgcg cgagaccgcc
     1261 gtgagctcgg tcatcgtcgc gcggccgatg gaggcggcct cctcggtcga gcgctcgtgc
     1321 tcgggcgtgg acgcgagcag cgccgcggcc gtgacctgct cgaggtggcg ctgcgcgatc
     1381 gagacgtcgg cgtagtggcc ggcgatgacc tcgccctgtt cggtgaactt cagccgcccg
     1441 ccgaccgagc ccggcgcctg ggcaaggatc gcccggttcg cgggtccgcc accgcggccg
     1501 agggccccgc cacgcccgtg gaacagcgtc agtgcgatgc cgtgtcgccg ggacgtcgcg
     1561 atcagcgcct cctgcgcccg gtagaggagc cagttcgcgg ccaggacgcc gctctccttc
     1621 gacgagtccg agtagccgag catcacctcc tgcctgtcac cacgctcccg caggtgggcg
     1681 cgatacgccg ggtcgtcgag caggccgtcg agcagctccg tcgcgccggc gagggcgtcg
     1741 gcgctctcca gcaggggcac gacgtcgagg acggggaggt ccggtggcag gtcggcgagc
     1801 gccgggacgg ggcggccgaa cggctccgtc tgggcggcga gccgcgcgag ctcgaggaca
     1861 tcggcgacgt cgtcggggct ggtggtgaac gagatcacgt accggcggca tgcctcgacg
     1921 ccgaagcgcg cctggagccg ggcgatcgag cggaacgtgg ccaggacctc ggccacggtg
     1981 acgccggcgg cgacctcgac gtccggcccg gcgtccgagc gcagcgcccg cagggccgcg
     2041 gcgtggaccg ccgcgtgctg gcggacctcg agcgaggcga ggtggcaccc gaccgtcgcc
     2101 agcggccacc gcagctcggc gacctcgccc caggccaccc ggcccagcgc atcctcga
//
LOCUS       DBNHXO010000084         1619 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2204143, whole genome shotgun sequence.
ACCESSION   DBNHXO010000084 DBNHXO010000000
VERSION     DBNHXO010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2204143"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /locus_tag="AC162A_01795"
     CDS             complement(<1..1426)
                     /locus_tag="AC162A_01795"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR5009597.1"
                     /translation="MKSRAAIAPVVAVLVALNVAACGQATQSPSPASSPAASAVPAAS
                     PTVSSTPRPSAEPPSAANPVPVIVDTDLAADDLYALLVLLRDPAVDVRAVTIAGTGEV
                     HCVPGLRNTRRLLAALGRPEVPVACGREQPGPNGRWFPLEWRAGSDAFYGVELPPVDG
                     ETARNEEAPQMLVRLTAESDVPLTLVTLGPLSNVADAAALDPAFASRLAGIHAMAGTI
                     DAPGNIELGDTAPSDGVEWNVGADPDAMGAVLTLDVPITLVGLDATNDVPVPPDIVDQ
                     LAADHAAAGADIVFEMYATSPFLSSSGNSYWDPLAAVTLTDPSIAGWEDVTVRMEPAG
                     AGAGRLVRDPGGRPVRAAMSADGDAFMTRFLGALRTGEPRANPFAVVGSLAVRWDGEA
                     CTVVGEPPAGPGLVKVVLENTSTQEVALSVGEVKPPRTWDDVLAFANEADLTDPNLVI
                     PDWIVPVPLELGAPGGSSAAAYAPM"
BASE COUNT          239 a          588 c          592 g          200 t
ORIGIN      
        1 gcatcggggc atacgccgcg gcgctcgatc ctcccggggc cccgagctcg agcggaaccg
       61 ggacgatcca atcggggatc acgaggttcg ggtccgtcag gtcggcctca ttagcgaatg
      121 ccaggacgtc gtcccaggtc ctggggggct tcacctcgcc gacgctgagc gcgacctcct
      181 gggtcgacgt gttctcgagc acgaccttca cgagcccggg gccggccggc ggttcgccga
      241 cgaccgtgca ggcctcgccg tcccagcgga ccgccagcga gccgaccacc gcgaacgggt
      301 tcgcgcgtgg ctcaccggtc cgcagcgcgc ccaggaagcg cgtcatgaag gcgtcgccgt
      361 cggcggacat cgccgcgcga acgggccgcc cgcccgggtc gcggacgagc cgccccgccc
      421 cggcgcctgc cggttccatg cggaccgtca cgtcctccca gccggcgatc gacgggtcgg
      481 tgagcgtgac cgccgccagc gggtcccagt aggagttccc gctggaggag aggaacggcg
      541 acgtcgcgta catctcgaac acgatgtcag cacccgccgc ggcgtggtcg gcggccagct
      601 ggtcgacgat gtcgggtggc acgggcacgt cgttggtcgc gtcgaggccg acgagcgtga
      661 tcgggacgtc gagcgtcagg accgcaccca tcgcatccgg gtccgcgccc acgttccact
      721 cgaccccgtc tgagggcgct gtgtcgccga gctcgatgtt gcccggggcg tcgatcgtgc
      781 ccgccatcgc gtgaatgccg gcgaggcgcg aggcgaacgc gggatcgagg gccgcggcgt
      841 cggccacatt cgagagcggc ccgagggtca cgagcgtgag cggcacgtcg ctctcggcgg
      901 tgaggcgcac gagcatctgc ggcgcttcct cgttgcgcgc cgtctcaccg tcgaccgggg
      961 gcagctcgac gccgtagaac gcatcggatc ccgcccgcca ctcgagcggg aaccatcgcc
     1021 cgttcgggcc cggttgctca cggccgcagg cgaccgggac ctctggccga cccagggcgg
     1081 cgaggagccg gcgggtgttg cggaggccgg gcacacagtg aacctccccg gtccccgcga
     1141 tcgtgaccgc acgcacatcg acggcggggt cacgcagcag gacgagcagc gcgtacaggt
     1201 catccgccgc gaggtccgtg tcgacgatca caggcaccgg gttggccgcc gaaggcggct
     1261 cggcggacgg ccgaggagta gaggacaccg tcggcgaggc cgccggcacg gccgacgccg
     1321 cgggagacga ggcgggtgaa ggcgactggg tcgcttgccc gcaggcggcg acgttcaggg
     1381 cgaccagcac agccacgacc ggcgcgattg cggcgcggga cttcatgtcg tgcaccccgc
     1441 ctcctccagg cacgcgtccc tatggcgcga cggtgccccg gtcgaaccag atccgctcgc
     1501 cgccgaaccg ctgcgacgtc gaatcccacg ggaacatgat cagcacctgc aggctcaccg
     1561 gcaagcccga cgccggcacg cccgcccacg gcccgaggtt ggtgccggtc aactgcgcg
//
LOCUS       DBNHXO010000085         4258 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_813836, whole genome shotgun sequence.
ACCESSION   DBNHXO010000085 DBNHXO010000000
VERSION     DBNHXO010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_813836"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="AC162A_01800"
     CDS             complement(<1..478)
                     /locus_tag="AC162A_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5009598.1"
                     /translation="MSNEGAVVVVGGTRAIGLELVRHYAAQGREVVLSGQSRENVDAA
                     VAEIGGSVRGLTFDLSEPHTIAAALADVGAVDKLALVAIDRDQNTIADYDIDRAIRLV
                     TLKLVGYTAVVSALRPRLTESSSVVLFGGMAKERPYPGSTTVTTVNGGVVGLTRTLV"
     gene            complement(468..1385)
                     /locus_tag="AC162A_01805"
     CDS             complement(468..1385)
                     /locus_tag="AC162A_01805"
                     /inference="COORDINATES: protein motif:HMM:NF014376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5009599.1"
                     /translation="MHSVGRIDWSFADHPTPASARSSGLARVVLVGPEQGAVHTELAA
                     GAFVPGGWLRRHVHSFEEALYVLAGELAIEIGGTAHRLVAGDFALMPIGTWHALASAG
                     EGQVRWLSVSTPQRLTPDAGRKDTFFTRGTFDPADLVARAARPRFGDPAVRLVGHYDG
                     TPPQVEALRVTDPARGRKPAGMDTAILAYSGISVKMLVDKVFGADMLTMFTVDYELNG
                     SAQAHDHPFEETYVFLAGEVEAELDGEHHTLRPGDVVFAGVGSLHGFYNTGTERVRWL
                     ETQAPQPPVRHAYRWAPTWTKFEEDNDVE"
     gene            complement(1409..2389)
                     /locus_tag="AC162A_01810"
     CDS             complement(1409..2389)
                     /locus_tag="AC162A_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009600.1"
                     /translation="MTETTSAVEAPGPSATSRARALIGRGGIVIPFVIAFVVLSLISP
                     PFLRFQNLANILDQQSGIIIVAAAGTLVLIAGGIDLSVGAIYGLAGATAAQLAVSFGP
                     PVGIIAAILVGLMIGLSNGVIVTRFRINPLIGTLAMTFVVGGIGAIATHGNLIVVFDH
                     PEFKVFAATKIAGISSAAWMMIAFAIATAILLSRTTFGRYVYATGGNAEAARLGGVRI
                     NTVRVATYALSGAAAALAGTLDASRVLSAQASAGDFLTFTVLTGIIVGGTSIMGGEGS
                     VQRTVIGCLFVALIANGFNLLGLDPYYQQITLGIILLLAVGTDAWSRRQA"
     gene            complement(2386..3924)
                     /locus_tag="AC162A_01815"
     CDS             complement(2386..3924)
                     /locus_tag="AC162A_01815"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR5009601.1"
                     /translation="MAGLTSNQAAPAVAPDGAHLEILEVGKSFGGTRALDDVSVTITA
                     GSVHAFVGENGAGKSTLGKIVAGVFPPDQGQLALRGEIVTFRSPREALARGIALVAQE
                     VAVVPRRTVAENVFLGTEPRRFGFVQRRRLLERFDRLVAGAGFELPADAIVGSLPLAK
                     QQQVEILRALARDAELIVFDEPTAALSAIEVQRFHEIVRTLTRSGRSVILVSHFLGEV
                     LALADTITVLRDGKLVKTGPAADETEDTLVAAMLGRSVSRTYPPKQVPPADAPVALTV
                     RDLTAAGVSGASLEIRAGEIVALAGLVGAGRTELARAIFGASPATAGEMTAGTSRLTG
                     TPLGSLRANVSMIPESRKDDGLLLRRPVRENVSLASLPSLGRFGFVRRGVEATRAREA
                     LRKVAAADNLEATPVTLSGGNQQKLMFARALLNKPAVLIADEPTRGVDIGAKRDIYEL
                     IVGLAAGGVAILLISNEIEEVLGLAHRVVVMRGGRVVAEVSGDNITEEAILAASFGRT
                     SSAA"
     gene            complement(3912..>4258)
                     /locus_tag="AC162A_01820"
     CDS             complement(3912..>4258)
                     /locus_tag="AC162A_01820"
                     /inference="COORDINATES: protein motif:HMM:NF024799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR5009602.1"
                     /translation="MLTAHPEINVITGADQAITGALQAVQDASLADKVKLVGYGGGDV
                     AFKGIKSGERFGTVMQAPATEGKLGTEQFIQAIRTGVPAAGVDVLANLPDGGVVTQEN
                     VDKFLTLAEWPG"
BASE COUNT          621 a         1489 c         1563 g          585 t
ORIGIN      
        1 cgaccagcgt ccgggtgagg cccacgacgc cgccgttgac ggtggtgacc gtcgtggagc
       61 ccgggtacgg ccgctccttg gccatgccgc cgaacaggac gacggacgag gattcggtca
      121 gccgcggtcg gagcgcgctg acgacggccg tgtagccgac gagcttgagc gtgaccagcc
      181 ggatcgcccg gtcgatgtcg tagtcggcga tggtgttctg gtcgcgatcg atcgccacca
      241 gggcgagctt gtcgacggcg ccgacgtcgg ccagcgccgc cgcgatcgtg tggggctcgg
      301 agagatcgaa cgtcaggccg cgcacggacc cgccgatctc ggccacggcc gcgtcgacgt
      361 tctcccggga ctggccggac aggacgacct cgcggccttg cgccgcatag tggcggacca
      421 gctccagccc gatcgcccgc gtcccgccga cgacgacgac ggctccctca ttcgacatcg
      481 ttgtcctcct cgaacttcgt ccatgtcggc gcccagcggt acgcgtggcg gaccggcggt
      541 tgcggcgcct gggtctcgag ccaccggaca cgctccgtgc cggtgttgta gaagccgtgg
      601 aggctgccca cgcccgcgaa cacgacatcg ccagggcgga gcgtgtggtg ctcgccgtcc
      661 agctcggcct cgacctcgcc cgcgaggaac acgtaggtct cctcgaacgg gtggtcgtgc
      721 gcctgggctg agccgttgag ctcgtagtcg accgtgaaca tcgtgagcat gtccgcgccg
      781 aacaccttgt cgacgagcat cttcacggag atcccgctgt aggcgaggat cgcggtgtcc
      841 atcccggccg gcttgcggcc gcgcgcggga tccgtgaccc gcagcgcctc gacctggggt
      901 ggcgtgccgt cgtagtggcc gaccaggcgc acggccgggt cgccgaaccg ggggcgcgcc
      961 gcccgtgcca cgaggtcggc cgggtcgaac gtcccgcggg tgaagaacgt gtccttgcgt
     1021 ccggcgtccg gcgtcaggcg ctggggcgtg ctgaccgaca gccagcgcac ctggccctcg
     1081 ccggcgctgg cgagcgcgtg ccaggtcccg attggcatca gggcgaagtc cccggcgacc
     1141 agccggtggg cggtcccgcc gatctcgatc gcgagctcgc ccgccaggac gtacagcgcc
     1201 tcctcgaacg agtgcacgtg ccgccgcagc cagcccccgg gcacgaacgc gccggccgcc
     1261 agctccgtgt ggacggcccc ctgctcaggc cccacgagga cgacgcgggc cagccccgac
     1321 gagcgggccg atgccggcgt gggatgatcg gcgaatgacc agtcgatgcg gccgacggag
     1381 tgcacggatc cccctgtgtt agctgcgctc aggcctggcg gcgcgaccag gcatctgtcc
     1441 cgaccgcgag gaggaggatg atcccgagcg tgatctgctg gtagtacggg tcgaggccga
     1501 ggaggttgaa gccgttcgcg atgagcgcga cgaacaggca gccgatgacc gtccgctgga
     1561 ccgagccctc gccgcccatg atgctcgtcc cgccgacgat gatgccggtg agcaccgtga
     1621 acgtcaggaa gtcgcccgcc gacgcctggg cggagagcac gcgcgacgcg tcgagcgtcc
     1681 cggcgagggc cgctgcggcg ccgctgagcg cgtaggtcgc gacgcgaacg gtgttgatcc
     1741 gcacgccgcc cagccgcgcg gcctcggcgt tgccgccggt cgcgtagacg tagcggccga
     1801 acgtggtccg gctgagcagg atcgccgtgg cgatggcgaa cgcgatcatc atccaggccg
     1861 ccgacgagat cccggcgatc ttcgtggcgg cgaagacctt gaactcggga tggtcgaaca
     1921 ccacgatcag gttgccgtgc gtggcgatcg cgccgatgcc gccgacgacg aacgtcatcg
     1981 cgagggtgcc gatcagcggg ttgatccgga agcgggtgac gatgacgccg ttcgagaggc
     2041 cgatcatcag cccgacgagg atcgccgcga tgatcccgac cggcgggccg aacgacaccg
     2101 cgagctgcgc cgctgtcgcg ccggcgaggc cgtagatcgc cccgaccgag aggtcgatac
     2161 cgcccgcgat cagcaccagc gttccggcgg cggcgacgat gatgatcccg ctctgctgat
     2221 cgaggatgtt ggcgaggttc tggaagcgca ggaagggcgg gctgatcagg ctcaggacga
     2281 cgaacgcgat cacgaacggg atgacgatgc cgccacgccc gatcagggcg cgcgcgcggc
     2341 tggtcgcgct ggggcccggc gcctcgacgg cggacgtggt ctcggtcatg cggcgctcga
     2401 ggtccggccg aacgaggcgg ccaggatcgc ctcctcggtg atgttgtcgc ccgacacctc
     2461 cgcgacgacg cgcccgccgc gcatgacgac gacccggtgc gcgagcccca gcacctcctc
     2521 gatctcattg gagatcagca ggatcgcgac gccgccggcg gcgagcccga cgatcagctc
     2581 gtagatgtcg cgcttcgcgc cgatgtccac gccgcgggtc ggctcgtcgg cgatcagcac
     2641 cgcaggcttg ttgaggaggg ctcgggcgaa catgagcttc tgctggttgc cgcccgacag
     2701 cgtcacgggg gtcgcctcga ggttgtccgc cgcggcgacc ttccggagtg cctcgcgggc
     2761 acgggtggcc tcgacgcccc gccggacgaa cccgaaccgg ccgagcgacg ggaggctcgc
     2821 gaggctgacg ttctcgcgca ccggccggcg cagcagcagg ccgtcgtcct tgcgggactc
     2881 ggggatcatc gagacgttgg cccggaggga ccccaggggc gtgccggtga ggcggctggt
     2941 gccggccgtc atctcgccgg cggtggccgg tgacgcgccg aagatcgccc gggcgagctc
     3001 cgtgcgcccc gccccgacga gtcccgcgag ggccacgatc tcgccggccc ggatctccag
     3061 cgacgcaccg gacacgcccg cggccgtcag gtcgcggacg gtgagggcga cgggcgcatc
     3121 cgcgggcggc acctgcttgg gcgggtaggt ccggctgacc gaccggccga gcatcgccgc
     3181 gacaagcgtg tcctcggtct catccgctgc cggtccggtc ttgaccagct tgccgtcccg
     3241 caggaccgtg atcgtgtcgg cgagggccag cacctcgccc aggaagtggg acacgaggat
     3301 caccgaacgc ccgctccggg tcagcgtccg cacgatctcg tggaaccgct ggacctcgat
     3361 cgccgacagg gccgcggtcg gctcgtcgaa cacgatcagc tcggcatcgc gggcgagggc
     3421 acgcaggatc tcgacctgct gttgtttggc caggggaagg gaccccacga tggcatcggc
     3481 cgggagctcg aagccggcgc cggcgaccag acggtcgaac cgctcgagca ggcggcgacg
     3541 ctggacgaat ccgaagcgcc gcggctcggt gcccaggaac acgttctcgg cgacggtgcg
     3601 ccggggaacg acggcgacct cctgggcgac gagcgcgatg ccccgcgcga gcgcctcgcg
     3661 cggggaccgg aacgtgacga tctcgccgcg cagcgcgagc tggccctgat cgggcgggaa
     3721 gaccccggcc acgatcttgc ccagggtgga cttgccggcc ccgttctcgc cgacgaaggc
     3781 atggacggag ccggcagtga tcgtgaccga gacgtcgtcg agcgcgcgcg tcccgccgaa
     3841 cgacttgccc acctcgagga tctcgaggtg ggcgccgtcc ggggccacgg cgggcgccgc
     3901 ctggttggag gtcagcccgg ccactccgcg agggtcagga acttgtccac gttctcctgc
     3961 gtgaccacgc cgccatcggg caggttggcg aggacgtcga cgcccgccgc cggcacgccg
     4021 gtccggatgg cctggatgaa ctgctcggtg cccagcttgc cctccgtcgc gggggcctgc
     4081 atgacggtgc cgaatcgctc gcccgacttg atgcccttga acgcgacgtc accgccgccg
     4141 tagccgacga gcttgacctt gtcggccagg ctcgcgtcct gaacggcctg cagcgcgccg
     4201 gtgatcgcct ggtcggcgcc ggtgatcacg ttgatctcgg ggtgcgcggt cagcatgt
//
LOCUS       DBNHXO010000086         3272 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5180474, whole genome shotgun sequence.
ACCESSION   DBNHXO010000086 DBNHXO010000000
VERSION     DBNHXO010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5180474"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="AC162A_01825"
     CDS             complement(<1..1116)
                     /locus_tag="AC162A_01825"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012343.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR5009603.1"
                     /translation="MATTTLPHAAPAYRSRLYEWLTTTDHKKIGILYLVNSLIFFFAG
                     GLLALVVRLQLVTPLDATTMGGLVSQDFYNQAFTMHASFMLFLFIIPILAGFGNYAVP
                     LQIGAPDMAFPRINALSFWLLPLGGLLMASGFMASGGAAASGWTEYPPLSGPKYSGTG
                     TDLWIMGLTLIGTSSILGAINFLVTIFKMRAPGMTLFRMPILVWTVLVTSVLVVMATP
                     VLTSALIMLFIDRNYGGTFFEPSIGGNAVLYQNIFWFYSHPAVYIMVLPAMGIVSEVL
                     PVFSRKPLFGYKAFVFATVGIGALGFSVWAHHMFTTGQVFLPFFSLMTFLIAVPTGVK
                     MFNWIFTMWRGQLMLKTPLLFALGFLSMFLIGGINGAF"
     gene            complement(1130..2251)
                     /gene="coxB"
                     /locus_tag="AC162A_01830"
     CDS             complement(1130..2251)
                     /gene="coxB"
                     /locus_tag="AC162A_01830"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR5009604.1"
                     /translation="MSMPPRSRTGSGPLIAGTLILLAIGIGVVVLLASGITPQRLWDS
                     FFPIENQTPVTDRSLAIKALYDFVFYIAAAIFVIVEAVLIYTAFRYRRKPTDAELPPQ
                     THGNNLVEVVWTVIPTIIVAVLFVLSWQTLNTVDAKATTTVHITAVAARFQWSFDYLA
                     ADGKTVLFTQNLPKGDEGGMFVPVGEPVQVDLRSTDVIHAFYVPKFLFKRDVVPGKLN
                     TFDFTVEEAGVYRGQCAELCGTYHGSMLFEVHAVDRATFDAWLQKQIDLANQTPAPPP
                     SGGAGGETLPLSAQNTTFSTDALAATADAPFTIEFNNEDVGMPHNVEIKDPGGAQVFL
                     GKIITGPAKEPYPVPPLKAGSYTFVCTVHPNMTGTLTVQ"
     gene            2424..>3272
                     /locus_tag="AC162A_01835"
     CDS             2424..>3272
                     /locus_tag="AC162A_01835"
                     /inference="COORDINATES: protein motif:HMM:NF021214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HIR5009605.1"
                     /translation="MSIRVSSSVRRAPVVIAAAISVLALAPGALAHGGTVPVPPDAAA
                     LVFGWSFDPLAWLPAIVALLVWRQGVARVNRAHPDHPVATRRSIYWVAGVLVILFAVD
                     SGLAAYDTTLFSMHMVQHLLLTMVGPPLLLLAGPITLLLQASSPATRKRYILPVLHSS
                     IVRRVSHPVVAWIVFAAVMWASHFSPLFDLALENTWVHRLEHALFLGAALLFWWPVVG
                     PDPSPWKLRPPASILYVGLQMPQNTFLALAIYMAGAPLYLHYATTTRTWGPTPLEDQQ
                     FAGGIMW"
BASE COUNT          533 a         1071 c         1141 g          527 t
ORIGIN      
        1 gaacgccccg ttgatgccgc cgatgaggaa catgctcagg aagccgagcg cgaagaggag
       61 cggcgtcttg agcatcagct ggccgcgcca catcgtgaag atccagttga acatcttcac
      121 gccggtcggg accgcgatca ggaacgtcat cagggagaag aacggcagga acacctggcc
      181 ggtcgtgaac atgtggtgtg cccagaccga gaagccgagg gcgccgatgc cgaccgtcgc
      241 gaacacgaac gccttgtagc cgaacagcgg cttgcgcgag aagaccggca gcacctcgga
      301 cacgatgccc atcgccggca gcaccatgat gtagaccgcg gggtgcgagt agaaccagaa
      361 gatgttctgg tacaggaccg cgttgccgcc gatcgacggc tcgaagaacg ttccgccgta
      421 gttgcggtcg atgaacagca tgatcagcgc gctggtcagg accggcgtcg ccatcacgac
      481 gaggacgctg gtcacgagca cggtccagac gaggatcggc atcctgaaca gcgtcatccc
      541 gggcgcgcgc atcttgaaga tcgtgaccag gaagttgatg gcgccgagga tcgagctcgt
      601 gcctatcagg gtgagaccca tgatccacag gtccgtcccg gtgccgctgt acttggggcc
      661 cgagagcggc gggtactccg tccagcccga cgccgccgcg ccgcccgagg ccatgaagcc
      721 cgacgccatc agcagtccgc ccaggggcag cagccagaac gagagggcgt tgatccgcgg
      781 gaacgccatg tccggcgccc cgatctggag cggcacggcg tagttcccga agccggccag
      841 gatcgggatg atgaacagga acagcatgaa cgaggcgtgc atcgtgaacg cctggttgta
      901 gaagtcctgg ctcacgaggc cacccatggt ggtcgcgtcc agcggggtca cgagctggag
      961 ccgcacgacg agggcgagga ggccgccggc gaagaagaag atcagcgagt tgacgaggta
     1021 caggatcccg atcttcttgt gatcggtggt cgtcagccac tcgtacagcc ggctccggta
     1081 ggcgggagcg gcgtgcggca gcgtggtagt ggccatcccg tcagtgctcc tactggaccg
     1141 tgagggtgcc ggtcatgttc gggtggacgg tgcacacgaa ggtgtacgag ccggccttga
     1201 gcggcgggac cgggtacggc tccttcgccg gcccggtgat gatcttgccc aggaacactt
     1261 gggcgccgcc cggatccttg atctccacgt tgtggggcat ccccacgtcc tcgttgttga
     1321 actcgatggt gaacggcgcg tcggccgtgg ccgcgagcgc atccgtcgag aacgtcgtgt
     1381 tctgggcgct cagcggcagg gtctcaccgc cggcgccacc cgacggcggc ggggcggggg
     1441 tctggttggc gaggtcgatc tgcttctgga gccaggcgtc aaaggtggcc cggtcgacgg
     1501 cgtggacctc gaacagcatc gagccgtggt aggtgccgca caactcggcg cactggccgc
     1561 gatagacgcc ggcctcctcg acggtgaagt cgaacgtgtt gagcttgccc ggcacgacgt
     1621 cgcgcttgaa caggaacttg ggcacgtaga aggcgtggat cacgtcggtg gaccggagat
     1681 cgacctggac gggttcgcca accgggacga acatgccgcc ctcatcgccc ttgggcaggt
     1741 tctgcgtgaa cagcacggtc ttgccgtcag cggcgaggta gtcgaacgac cactggaagc
     1801 gcgcggcgac ggccgtgatg tggacggtcg tggtcgcctt ggcgtcgacg gtgttgagcg
     1861 tctgccacga caggacgaac aggaccgcga cgatgatggt cgggatgacg gtccagacca
     1921 cctcgacgag gttgttgccg tgggtctggg gcggcagctc cgcgtcggtc ggcttgcgcc
     1981 ggtagcggaa cgcggtgtag atcagcacgg cctcgacgat cacgaagatc gccgccgcga
     2041 tgtagaacac gaagtcgtac agcgccttga tcgccagcga gcggtcggtc accggcgtct
     2101 ggttctcgat cgggaagaag ctgtcccaca ggcgttgcgg cgtgatgcca ctcgcaagga
     2161 gcacgaccac gccgatgccg atcgccagga ggatcagcgt gccggcgatg agcgggccgg
     2221 acccggtccg tgaccgggga ggcatcgaca tcgggttcct cgaatgtcga cccggccgca
     2281 gcgtcacggt cgggccagca gtgggtctcg ccgtccgagg acgagcgcct cccgtgcggg
     2341 gctcgggacg tcgagatcat acccgacgac ggctgccgcg cgggccgtca gcccccttcg
     2401 ccgcgaaggg cgagaatgcc cgggtgagca tccgcgtttc ctcgtcggtt cggcgcgccc
     2461 cggtcgtcat cgcggcggcc atctcggtgc tcgccctcgc gcccggcgcg ctcgcccacg
     2521 gcggaaccgt ccccgtcccg cccgatgccg ccgcgctcgt cttcggctgg tcgttcgacc
     2581 ccctcgcctg gctgcccgcg atcgtcgcgc tgttggtgtg gcggcagggg gttgcgcggg
     2641 tgaatcgtgc ccatcccgat cacccggtgg cgacgcgccg ctccatctac tgggtggcgg
     2701 gggtgctcgt catcctgttc gccgtcgact ccgggctcgc cgcgtacgac acgacgctgt
     2761 tctcgatgca catggtccag cacctgctgc tgacgatggt cgggccgccg ctcctgttgc
     2821 tggcggggcc gatcaccctg ctcctccagg catcgtcgcc cgccacgcgc aagcgctaca
     2881 tcctgcccgt gctgcactcc tcgatcgtcc gccgcgtgtc ccatcccgtc gtcgcgtgga
     2941 tcgtgttcgc ggccgtgatg tgggcgagcc acttctcgcc gctgttcgac ctcgcgctcg
     3001 agaacacgtg ggtccaccgg ctcgagcacg ccctgttcct cggcgcggcg ctcctgttct
     3061 ggtggcccgt cgtcggaccc gacccgtcgc cctggaagct gcggccgccg gccagcatcc
     3121 tgtacgtcgg gctgcagatg ccccagaaca cgttcctcgc gctcgcgatc tacatggccg
     3181 gcgcaccgct ctacctccac tacgcgacga cgacccgaac ctggggcccg acgccgctcg
     3241 aggaccagca gttcgcgggc gggatcatgt gg
//
LOCUS       DBNHXO010000087         6558 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_220785, whole genome shotgun sequence.
ACCESSION   DBNHXO010000087 DBNHXO010000000
VERSION     DBNHXO010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_220785"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="AC162A_01840"
     CDS             <1..314
                     /locus_tag="AC162A_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009606.1"
                     /translation="GDKVSPDDRAAVESQVVTLREALKGSEVSAINSGMQALVETLSR
                     VSTAAYQASSASSDANGSSSDGASGPAEGEGDGEGEPAGARSGGGDAGDETVEGEFKE
                     V"
     gene            complement(386..1762)
                     /locus_tag="AC162A_01845"
     CDS             complement(386..1762)
                     /locus_tag="AC162A_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009607.1"
                     /translation="MTAETRFDRELPAILEDLYLGPSPDYRNEVMAAAVRTRQRPSWT
                     FAGRWFPMADIASRSAFAPRVPWRTIGMAVLIVALALAAVALVVGSRQTKVPPPFGPA
                     ANGLITYSADGDIYTVDPTSGLATAIVTGPTADSEPVFSHDGRRVAFRRQSTTSASPA
                     DDIVVVNADGSHPVVVNAQPFPGGSGGFEWAPDSRSLIVNATARGSVGSATFGTQADG
                     SAVWLFDADAVTAPRALAVDGASAYLAAARPPDGKAILINRPIGDDQQLLVLDVTTGK
                     ETLLATGGAGDIGQARWSPDGSRVVYSAKPADDLESERLFIVNADGTGARQITSAPGT
                     WFDIDMAWSPDGSQIAFLRYERVGEDDWRIRPIGIYSVADGSVRDVGPLAAEVRSQAP
                     NPADQYATTGEGMFIDWSPDGAMILAYPSEATGHPVLIDPVDGTWTIVPQLTTPKEPS
                     QAWQRLAP"
     gene            complement(1759..2322)
                     /locus_tag="AC162A_01850"
     CDS             complement(1759..2322)
                     /locus_tag="AC162A_01850"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5009608.1"
                     /translation="MDQRVLVERARQGDHDAFAQLVDPALARLDAAARLILRDPELAR
                     DAVQETLIRAWRDLRGLRDPDRFDAWLHRLTVNACLDLARSRRRRPIEVELNAIDAPT
                     ASDHAGTLADRELVDAALKRLDPGHRAVVALHYLLGMPLPDVAASLGIPLGTAKSRLH
                     HALAAMRRSAMAGSDQAPTHVPGGQVA"
     gene            complement(2532..3275)
                     /locus_tag="AC162A_01855"
     CDS             complement(2532..3275)
                     /locus_tag="AC162A_01855"
                     /inference="COORDINATES: protein motif:HMM:NF025120.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5009609.1"
                     /translation="MCDVDPVRVGLSIRALRRRRGWTQEELGRRAGLSRSAVSRIERG
                     EADRLSLRTLAKVTNVLGARIRLQVLWQGEEMDRLLDRDHAHLVEWVVRRLTADGWEV
                     APEATFQVNGERGSIDVFAWHPATGHLLVVEVKSVVPDVQATLAGVDRKARVAVAVAR
                     QRGWPPGPVSRVLVLPDDRTARRRLSRFAATFDKAFPARTVELRRWFASPDGPIAGVL
                     FVSNATQAGARHRVGVMRASATHEVSRRR"
     gene            3400..3960
                     /locus_tag="AC162A_01860"
     CDS             3400..3960
                     /locus_tag="AC162A_01860"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5009610.1"
                     /translation="MQQLELVEQARRGDHDAFAALAGAAIPRLDALARLILRDPERGK
                     DATQEALVRAWRDLPSLRDPERFDAWLHRLLVHACMDEARKHRRHAFEVELTPIDHPT
                     IRDSAISVAERDALERGFRKLDPEQRALIVLHHYLGLSLPEVAETMRIPLGTAKSRLS
                     RALSALRAALEADARMTPEIVEGRFA"
     gene            3957..5225
                     /locus_tag="AC162A_01865"
     CDS             3957..5225
                     /locus_tag="AC162A_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5009611.1"
                     /translation="MTTRDGFTRLVDTWLAEEGVQTTPDYLDEVLDRTSTSRQRPAWL
                     SPGRWLPVDTTTLNPRAYSIPPAVRYGLVAVLLIIATVAALALYAGSQRPRVPAPFGP
                     ATNGRIYFDVDGAIVVTNADGSGRQTLDIGIAASGPYLSPDGTSLAFVSVDPVDMAGD
                     TIVVAGADGSNARPIAGADLRLDIDPAFNPTWSPDGAELAFGAFHDGAFKLFVAKADG
                     SGVRALGDRDLYGRQNPEWSTSGDWIAFMALPETGDPLLAVIRPDGSGERTLPTSPGA
                     GDGFRGWQLWAPDDTNRLLYGIGNTADGLGDAMATMDVNAGTETILSDVAGTVEHRGA
                     WSPDGKRIAFHLGDDIAVVNADGTGLVRLPDRLSGGSIGWSPDGEWIYGKSPSDREVS
                     AIDASGKRPPIVILLDGAETGVFSWQRIAP"
     gene            5385..6053
                     /locus_tag="AC162A_01870"
     CDS             5385..6053
                     /locus_tag="AC162A_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5009612.1"
                     /translation="MSRPDPAEQSRSRRERILDAAFHAFATQGYRDTAVDDIAGAAET
                     SKGGIYFHFPNKESIFRELMETTADKLVARVERAVAQESEPVARAEVAIHTVLATFAG
                     HRTMARLLFLDTMGAGRVFQTETNALHERFARLIQGYLDEAVTAEVIPPIDTRIASIA
                     WFGALNEVVARWLLADHAGRLEDAYPALRAILLRSVGVSEARIERVAVPAIADGADFP
                     EKAR"
     gene            6050..>6558
                     /locus_tag="AC162A_01875"
     CDS             6050..>6558
                     /locus_tag="AC162A_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009613.1"
                     /translation="MTAVRPVTGQTAHAAPALSMRLAALVEAATASVDTGLASVTASV
                     APLDSISLYAAAVEANLEAALWLRPAAGTSFVGVGRAWATEPAGPERFAEAEQAWRAL
                     LRTSRIDGASPVLMGGLGFTGRRPNDDDAWAPFGPASLVLPELLYASTLDGAALTASV
                     VGAADDIDGR"
BASE COUNT          968 a         2309 c         2316 g          965 t
ORIGIN      
        1 tgggcgacaa ggtgtcgccc gatgaccgcg cggccgtgga gtcacaggtc gtgacgctgc
       61 gcgaggcgct caagggctcc gaggtgtcgg cgatcaactc cgggatgcag gcgctggtcg
      121 agacgctgtc gcgggtgtcg accgccgcgt atcaggcgtc gtccgcgtcg tcggacgcca
      181 acggctcatc gtccgacggg gcctcggggc ctgccgaggg cgagggcgat ggtgagggcg
      241 agcccgccgg agcccgctcg ggtggcggcg acgccggcga cgaaacggtc gagggcgagt
      301 tcaaggaggt ctagcctccg ttcctccgtt catgcaaccg cccgccggct gacgccgacg
      361 ggcggttttg gttgtggcga cgggatcagg gcgcgagccg ttgccacgcc tggctgggct
      421 ccttcggcgt ggtgagctgg gggacgatcg tccaggtgcc atcgacgggg tcgatgagca
      481 cggggtgccc ggtggcctcg ctcgggtagg cgaggatcat ggcgccatcc ggcgaccaat
      541 cgatgaacat tccctccccg gtcgtcgcgt actggtcggc cggattcggc gcctgggaac
      601 gcacctctgc cgccagcggc ccgacatcgc ggaccgagcc gtcggccacc gagtagatgc
      661 cgatcgggcg gatgcgccag tcgtcctcgc caacgcgttc atagcgcagg aacgcgatct
      721 ggctgccgtc gggcgaccac gccatgtcga tgtcgaacca ggtcccgggc gcgctcgtga
      781 tctgccgggc gcccgtcccg tcggcgttga cgatgaacag ccgctcagac tcaaggtcgt
      841 ctgccggctt cgcgctgtag accacccgcg atccatccgg cgaccagcga gcctggccga
      901 tatcgcccgc gccgcccgtc gccaggaggg tctccttgcc cgtggtgaca tcgaggacca
      961 gcagctgctg gtcgtcgccg atcgggcggt tgatgaggat ggccttcccg tcgggcggcc
     1021 gagctgcggc gaggtaggcg gatgcgccgt cgacggcgag ggcgcggggt gccgtgacgg
     1081 cgtcagcgtc gaacagccag acggcgcttc cgtcggcctg cgttccgaag gtggccgaac
     1141 cgacggagcc gcgcgccgtc gcgttgacga tcagcgaccg ggagtccggc gcccattcga
     1201 agccgccact cccaccgggg aacggctggg cgttgacgac gaccgggtgc gaaccatccg
     1261 cattcacgac gacgatgtcg tcggcggggc tcgcgctggt cgtgctctga cgacggaacg
     1321 caacccgcct cccgtcgtgc gagaagaccg gttccgaatc ggccgttggg ccggtcacga
     1381 tggccgtcgc caaaccgctc gttggatcga cggtgtagat gtcaccgtcg gccgaatagg
     1441 tgatcaatcc gtttgccgca ggcccgaacg gcggcgggac cttggtctgg cgagatccga
     1501 cgacgagcgc caccgcggca agcgcgaggg cgacgatgag gacggccatc ccgatggtcc
     1561 gccacgggac tcgtggcgcg aacgcggaac ggctggcgat gtcagccatg gggaaccacc
     1621 ttccagcgaa ggtccatgac gggcgctgcc gcgtgcggac ggcggccgcc atgacctcgt
     1681 tgcgatagtc gggagacggc cccaggtaga ggtcctcgag gatggcgggc agttcgcggt
     1741 cgaaccgcgt ctcagcggtc atgcgacctg ccctcccggg acgtgcgtcg gggcctggtc
     1801 cgaacccgcc attgccgatc ggcgcatcgc cgcgagggcg tgatgcaacc gggacttggc
     1861 ggtcccgagc gggatgccca gggacgccgc cacgtctggc agtggcatcc ccaggaggta
     1921 gtgcagggcg acgaccgcgc gatggccggg atcgaggcgc ttgagcgccg cgtcgacgag
     1981 ctcgcggtcg gcgagagttc cggcgtggtc ggacgccgtg ggtgcatcga tggcgttgag
     2041 ctcgacctcg atcgggcgcc gtcgccggct ccgggcgagg tcgagacagg cattcacggt
     2101 gaggcggtgg agccaggcgt cgaaccggtc gggatcccga aggccccgca ggtcccgcca
     2161 ggctcggatg agcgtctcct ggaccgcgtc gcgcgcgagc tccgggtccc gaagaatgag
     2221 ccgggccgcc gcgtccagcc gcgcaagcgc agggtcgacg agctgggcga aggcgtcatg
     2281 atcgccctgc ctcgcgcgtt cgacgagcac tcgctggtcc acggtgacct cggtgtgcca
     2341 cgacctgggc ggtcgcgttc gtcagcgtat ggcgtcgagc aacccggaat cgttcacccc
     2401 gggccacttt cgcgcccgcg tgcccgcggc gagtcgtcgg cccccgatcc caaggctcgt
     2461 ttctgccatt cgctcatcac acgggcgttc gtcgcgacgt ttccgccaag cgctccgtcg
     2521 cggagcgggt gtcagcgtcg ccgactgact tcgtgcgtgg cgctggcacg catcaccccg
     2581 actcggtgac gagctcccgc ctgggtggca tttgacacaa acaggacgcc cgcgatcggg
     2641 ccatctggcg atgcgaacca ccgacgcagc tcgacggttc tcgcggggaa cgccttgtca
     2701 aacgtcgcgg cgaaccgcga gaggcgacga cgcgccgttc gatcgtccgg caggaccagg
     2761 acgcgggaga cgggacccgg cggccacccc cgctgtcgag cgacggccac ggcgactcgg
     2821 gccttgcggt cgacgcccgc cagcgtcgcc tgcacgtcag gaacgactga cttcacctcg
     2881 acgaccagca gatgcccggt tgccgggtgc catgcgaaga cgtcgatgga cccgcgttcg
     2941 ccattcacct ggaacgtcgc ctcgggcgcc acctcccagc cgtcggcggt gagccgtcgc
     3001 acgacccact cgaccaggtg cgcgtggtct cgatctaata ggcgatccat ctcctcgccc
     3061 tgccacagga cctggagccg aatccgggcc cccaggacgt tggtgacctt ggcaagcgtt
     3121 cgcagcgaga gccggtcggc ttcgccgcgt tcgattcggg acacggctga ccgggacagc
     3181 ccagcccgcc ggccgagctc ctcctgggtc cagccacgcc ttcgtcgaag cgccctgatc
     3241 gagaggccga ctcgtaccgg gtccacgtcg cacacgatgg gccggaccgc ttatccgcga
     3301 ctcaccagcg attttggaac ggtcgcccga ttcgatccgc catccttggc gagtgcggcc
     3361 acctgagcgt cgcaccggac tcattcgggt gagacggcag tgcaacagct ggagctcgtg
     3421 gaacaggcgc ggaggggcga tcatgacgcc ttcgccgccc tggcaggagc cgcgattcca
     3481 cgcctcgacg cgctcgcccg cctcatcctg cgcgacccgg aacggggcaa ggacgccacc
     3541 caggaggcgc tcgtgcgcgc ctggcgcgac ctgccgtcgc tccgcgaccc cgagcgattc
     3601 gacgcctggc tccaccgcct cctcgtccac gcctgcatgg acgaggcgcg gaagcaccgt
     3661 cggcacgcgt tcgaggttga gctgacacca atcgaccatc caacgatcag ggacagcgcc
     3721 atctccgtcg ccgagcgcga tgcgctcgag cgcggattca ggaagctcga tcccgagcaa
     3781 cgcgccctga tcgtcctgca tcactacctg ggcctgtcgc tgccggaggt ggccgagacg
     3841 atgcggatcc cgctgggaac cgccaagtcc cggctctccc gcgccttgtc ggccttgcgc
     3901 gctgcgctcg aggccgacgc caggatgacc cccgagatcg tggagggccg gttcgcatga
     3961 ccacgcgcga cggcttcacc cgactcgtcg acacctggct cgcagaggaa ggcgtgcaga
     4021 cgacgcccga ctacctggac gaggtcctgg atcggacgtc gacatcccgg cagcggccgg
     4081 cctggctcag ccccgggagg tggctgccag tggatacgac caccctcaac cctcgcgcct
     4141 actcgatccc gccggctgtc cgctacggcc tggtcgcggt gctgctcatc atcgccaccg
     4201 tcgccgcgct cgcgctgtac gccggctcac aacggcctcg cgtgccggcg ccgttcgggc
     4261 cggcgacgaa cgggcgtatc tacttcgacg tcgacggtgc catcgtcgtc accaacgccg
     4321 acgggagcgg ccggcagacg ctggacatcg gcattgcagc gtcgggtccg tacctgagcc
     4381 ccgacggcac gagcctcgcc ttcgtctccg tcgacccggt ggacatggcc ggcgacacca
     4441 ttgtcgtcgc cggggcggac ggctcgaacg cacgcccgat cgccggagcg gatctacgcc
     4501 tcgatatcga ccctgccttc aaccccacgt ggtcccccga cggcgccgaa ctcgcgttcg
     4561 gtgccttcca cgacggcgcc ttcaagctgt tcgtcgccaa ggccgatggc tccggcgtgc
     4621 gggcccttgg cgaccgcgat ctatacggac gccagaaccc ggagtggtcc acgtccggcg
     4681 actggatcgc gttcatggcc ctgccggaaa caggcgaccc gctcctcgcg gtcatccggc
     4741 ccgatggcag cggggagcgc accctgccaa cgtccccggg cgcgggcgac ggcttccgcg
     4801 gctggcagct gtgggctccg gacgacacca accgcctgct gtacggcatc gggaacaccg
     4861 ccgacggcct cggcgatgcc atggcgacga tggacgtcaa cgcggggacc gagacgatcc
     4921 tgtccgacgt cgctggcacg gtcgagcacc gcggtgcctg gtcgccggac gggaagcgga
     4981 tcgcgttcca cctcggtgat gacatcgcgg tcgtcaacgc ggatggaacg ggcctcgtcc
     5041 gcctccccga caggttgtcc ggcgggtcca tcggctggtc gcccgacggc gaatggatct
     5101 acggcaagtc acccagcgac cgcgaggtct cggcgatcga cgcgtccggc aagcgtccgc
     5161 cgatcgtgat cctgctagac ggcgccgaga ccggcgtctt cagctggcag cggatcgcgc
     5221 cgtagcggcg gcgtcgcccc gatcagaagg ccccggtcac cgccggggtc ttctcgcacc
     5281 cggttcgcga aataacgtgc gcattgacag taccaagttt ctggcgctag catggcgggt
     5341 ggaccgaccg actggtcggc cggttgcccc ggagcccgac cacgatgagc cgccccgacc
     5401 ccgccgaaca gagtcgcagc cgccgcgagc ggatcctcga cgcggcgttc cacgcgttcg
     5461 ccacgcaggg gtaccgcgat acggccgtcg atgacatcgc gggggccgcg gagacgagca
     5521 agggcggcat ctacttccac ttcccgaaca aggaatcgat cttccgcgag ctcatggaga
     5581 ccaccgccga caagctcgtc gcgcgggtcg agcgcgcggt ggcgcaggag tccgagccgg
     5641 tggcccgcgc ggaggtggcg atccacacgg tcctggcgac gttcgccggc caccgcacga
     5701 tggcgcggct cctgttcctc gacacgatgg gcgccgggcg cgtgttccag accgagacca
     5761 acgccctcca cgagcgcttc gcgcgcctga tccagggcta cctcgatgag gcggtcacgg
     5821 ccgaggtgat tccgccgatc gacacccgca tcgcgagcat cgcctggttc ggtgcgctga
     5881 acgaggtcgt tgcccggtgg ctgctggccg atcacgccgg gcggctcgag gacgcctatc
     5941 cggcgctgcg ggcgatcctg ctgcgatcgg tcggggtgtc cgaggcgcgg atcgagcgcg
     6001 tcgcggtgcc agccatcgcg gacggggccg acttcccgga gaaggcccga tgacggccgt
     6061 ccggccagtc accgggcaga ccgcgcacgc ggcaccggca ctcagcatgc gcttggcggc
     6121 gctggtcgaa gccgcgaccg cgtcggttga cacagggctc gcctccgtga ccgcgtccgt
     6181 ggcccccttg gactcgatct cgctctacgc cgcggcggtt gaggcgaatc tcgaggcggc
     6241 gctgtggctg cgcccggccg ctgggacgtc gttcgtgggc gtgggtcgcg cgtgggcgac
     6301 ggagcccgcg ggacccgaac ggttcgcgga ggccgagcag gcctggcgtg cgctgctgcg
     6361 gacttcccgg atcgacggcg cgagcccggt cctgatgggt ggcctcgggt tcacgggccg
     6421 ccggccgaac gacgacgatg cctgggcgcc gttcgggcct gcgtccctgg tgctgcccga
     6481 gctcctctac gcaagcacgc tcgatggcgc ggcactgacc gcgtcggtcg tcggcgcggc
     6541 ggacgacatc gacggccg
//
LOCUS       DBNHXO010000088         2033 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3196498, whole genome shotgun sequence.
ACCESSION   DBNHXO010000088 DBNHXO010000000
VERSION     DBNHXO010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3196498"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1779
                     /gene="typA"
                     /locus_tag="AC162A_01880"
     CDS             <1..1779
                     /gene="typA"
                     /locus_tag="AC162A_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018502179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR5009614.1"
                     /translation="FRANQAVVDRVMDSGDLEREKGITILAKQTTIDVDGVRLNIVDT
                     PGHADFGGEVERSLLMVDSVLLLVDAAEGPLPQTRYVLQKAMARRLPVVVALNKIDRS
                     DARAAEVLDDVYELFMDLGADEHQIEFPVVYTNAKLGTATRDLADPGTDLRPLLDLLV
                     DVTPAPVFEPDHPLQLLVTNLSANEYVGRMAVGRIRNGIIRVGQRIAVVREEADDTAG
                     EIEPGRMITFTGSVTSLQTARGMERIDISEAGPGEIVSVAGLPEVTIGDTLTEPGDPR
                     PLPRLDVDAPTLSMTFGVNTSPLGGRDGKYVTSRQIKARLEKEVLGNVSIEVHPTESG
                     DTFEVRGRGELQLAVLIEQMRREGYELTVSRPEVILRTVDGEVQEPYERITIDIPPDY
                     IGEVQQALAGRKGRLEQMTTDADGRVRMEFVLPVRGLIGYRGQLLTDTRGTALLHQIG
                     EGYGPWAGEVTHRTNGVLVSDRSGTSNAYGLFNLQERSELLIGAGEDVYEGMVVGENA
                     RAGDMDVNPAKEKKLTNIRTHSHDEALRLTPPRPLTLEAAIEFIGSDELVEVTPVAIR
                     LRKRLLAKTERERERGRAYDLARGGA"
     gene            complement(1787..>2033)
                     /locus_tag="AC162A_01885"
     CDS             complement(1787..>2033)
                     /locus_tag="AC162A_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009615.1"
                     /translation="LALAMDLSESTVRMVGGMFHAPDLGWPSGVQEDDDLHWSWAAQA
                     PTADGRQRVAEPPFEEVDPRSIRLRAQPLRRGTRQAP"
BASE COUNT          334 a          702 c          723 g          274 t
ORIGIN      
        1 ttccgcgcga accaggccgt cgtcgaccgg gtcatggact cgggcgacct cgagcgggag
       61 aagggcatca cgatcctggc caagcagacg acgatcgacg tcgacggcgt ccgcctgaac
      121 atcgtcgaca cgcccggcca cgcggacttc ggcggcgagg tcgagcgcag cctgctgatg
      181 gtcgacagcg tcctgctcct ggtcgatgcg gcggaaggcc cgctcccgca gacgcgctac
      241 gtgctgcaga aggcgatggc ccgaaggctt cccgtcgtcg tcgcgctgaa caagatcgac
      301 cgctcggacg cgcgcgcggc cgaggtcctc gacgacgtct acgagctgtt catggacctg
      361 ggcgccgacg agcaccagat cgagttcccg gtggtgtaca cgaacgcaaa gctcggcacg
      421 gcgacgcgcg acctcgccga cccgggtacg gacctgcgcc cgctgttgga cctgctcgtc
      481 gacgtcacgc ccgcgccggt cttcgaaccc gaccacccgc tgcagctcct ggtcaccaac
      541 ctgtccgcga acgagtacgt ggggcggatg gccgtcgggc ggatccgcaa cgggatcatc
      601 cgcgtcggcc agcgcatcgc cgtcgtccgc gaggaggcgg acgacaccgc gggtgagatc
      661 gagccgggcc ggatgatcac gttcaccggg tccgtcacga gcctccagac ggcgcgcggg
      721 atggagcgca tcgacatctc cgaggccggg ccgggcgaga tcgtctcggt cgccgggctg
      781 cccgaggtca cgatcggcga cacgctgacg gaaccgggcg atccacgccc gctgccgcgt
      841 ctcgacgtcg atgcgccgac gctgtcgatg acgttcggtg tgaacacgtc gccgctgggt
      901 ggcagggacg gcaagtacgt gacgtcgcgc cagatcaagg cccgcctcga gaaggaggtc
      961 ctgggcaacg tctcgatcga ggttcacccg accgagtccg gcgacacctt cgaggtccgg
     1021 ggccgcggcg agctccaact cgcggtcctg atcgagcaga tgcgccgcga gggctacgag
     1081 ctcacggtga gccgccccga ggtgatcctg cgcacggtcg acggcgaggt ccaggagcca
     1141 tacgagcgga tcacgatcga catcccgccc gactacatcg gcgaggtcca gcaggcgctc
     1201 gccggccgca agggccgcct cgagcagatg accaccgacg ccgacggacg cgtgcggatg
     1261 gagttcgtgc tgccggtccg ggggctgatc ggctatcgcg ggcagctcct cacggacaca
     1321 cgcgggacgg ccctgctgca ccagatcggc gagggctacg gcccttgggc gggcgaggtg
     1381 acccatcgca ccaacggggt gctcgtgagc gatcgatccg ggacgtcgaa cgcctacggg
     1441 ctgttcaacc tccaggagcg ctcggagctg ttgatcggtg ccggcgagga cgtctacgag
     1501 ggcatggtgg tcggggagaa cgctcgggcc ggcgacatgg atgtgaaccc ggccaaggag
     1561 aagaagctca ccaacatccg gacccattcc cacgacgagg cgctccggct cacgccgcct
     1621 cggccgctga cgctcgaggc ggcgatcgag ttcatcggca gcgacgagct ggtcgaggtc
     1681 acgcccgtgg cgatccgcct gcgcaagcgc ctgctcgcca agaccgagcg cgagcgcgag
     1741 cgtggccggg cctacgatct cgcccgcggc ggggcgtagg gtcgggctac ggcgcctgac
     1801 gtgtgccgcg acgcagcggc tgggcccgca acctgatcga ccgcggatca acctcctcga
     1861 acgggggctc ggcgacccgc tgccgcccgt cagcggtggg cgcctgagcc gcccaagacc
     1921 agtgcaggtc gtcgtcttcc tgcacgccgc tgggccagcc gaggtcgggg gcgtggaaca
     1981 tgccgccgac catcctgacc gtggattctg acaggtccat cgccagcgcc agg
//
LOCUS       DBNHXO010000089         3859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3990934, whole genome shotgun sequence.
ACCESSION   DBNHXO010000089 DBNHXO010000000
VERSION     DBNHXO010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3990934"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="AC162A_01890"
     CDS             complement(<1..736)
                     /locus_tag="AC162A_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HIR5009616.1"
                     /translation="MTSPTPTTDTRRASRAALFRDALFIALVLAIATAAVALVEAVAG
                     GDLDASSIYLVAVVIVATRLRTAAGIATAIAAFVIYNLLFIEPRFTLIVADAREWLNL
                     VLFLFVALVIGRLAGIGADRAEEAESRVAESTALYGITRVLASEGADTALPQVARSLV
                     ADAGMDRVWITLDDGGADRVAADSGNGAIGPSPAAVEVLMTDRGEPAWVRTHGPRTAA
                     DRRPAGSSGERRYKVRLESEGETVGAL"
     gene            786..1733
                     /locus_tag="AC162A_01895"
     CDS             786..1733
                     /locus_tag="AC162A_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR5009617.1"
                     /translation="MPSRTIPRRPGRPIGVGVQLPEVEREVRWPELRAIARTAEQAGF
                     DSIWFGDHLLYRYASGETRGPWEAWTTLAALAEATERVALGPLVAATAFHAPFMLAKQ
                     AATVDEISGGRLVLGLGAGWNDTEFAALGAPFDHRISRFEEAFTVVRTLLAEGAIDFA
                     GAYVQARDAELLPRPARPGGPLVLIGSSGPRMLGATMPYVDAWNAWYADTGNAPAGVA
                     ALRANVDAAAVAAGREPDAIERTVAVLVGMPGGNGRIMGDTDPRQAVPPLSGSPEAIA
                     EEFRGYAREGIGHVQLVVDPITEASVAALAPVLEALDRD"
     gene            1809..2306
                     /locus_tag="AC162A_01900"
     CDS             1809..2306
                     /locus_tag="AC162A_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR5009618.1"
                     /translation="MSDAQPRTRTRSASGRAEVSDLDKRIIEHLQQDGRRPYTQIASD
                     LGVSEAAVRARTNRLIEKGILQVVGVTDPLKLGFQQWAMIGVRVEASRLIEVAEEIAA
                     FPETDYVVVTAGSYDILVEAVAEDNEALLQFLSERLRKIPGVRETETFVYLRLMKQSY
                     QWGTR"
     gene            2434..3660
                     /locus_tag="AC162A_01905"
     CDS             2434..3660
                     /locus_tag="AC162A_01905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR5009619.1"
                     /translation="MPIETLLALLGIALLAFLVLIVVSPTPIGERLRRTAGRTRDEEL
                     MRGEPSAPGDLRPAALYRVIRVSTWVFIFAAATIVAVTDLWSTQQGPILLVLAAAAIT
                     LLVIQPLRGRIRETLLLTIEGAIGLVLAGMLIALTGGALSPFAFTLALIVVGAAIVVT
                     PDAALLLTAAAGATYLAAMAFDAQTRSGPPTEIAVATAGINLTALCLIAYVGMSIGRE
                     QRRAREDANRQATEDPLTGLRTRPFLFAALDREVDRSRRTGRSFCLLMLDLDDLKAIN
                     DSMGHLHGDRALRLVGDVVRNGIRRIDTGARFGGDEFVVLLPETDPTGGWVLAEKIRQ
                     GVAESDLAVDGVPIPTSVSVGVVTYPQDGLTVRALLECADAAMYRSKREGRDRVSGVP
                     VMDPDPSLTGSRSRPV"
     gene            3670..>3859
                     /locus_tag="AC162A_01910"
     CDS             3670..>3859
                     /locus_tag="AC162A_01910"
                     /EC_number="4.4.1.11"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of methanethiol and
                     2-ocobutanoate from L-methionine; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase"
                     /protein_id="HIR5009620.1"
                     /translation="MTSKRPGPGPSTRAIRAASRQPVVRQSPTSVPIYQTATFSSADA
                     EELAAVLTEEQQGYAYSRI"
BASE COUNT          530 a         1369 c         1433 g          527 t
ORIGIN      
        1 acagcgcccc gacggtctcg ccctcggatt cgagccggac cttgtagcgg cgttcgccgc
       61 tggaacccgc cgggcgtcgg tcggcggcgg tgcgcggacc gtgcgtccgg acccaggcgg
      121 gctcgccccg atcggtcatc agcacctcga cggcggcggg cgacggaccg atcgctccgt
      181 ttccgctgtc cgcggccacg cgatcggcgc cgccgtcgtc gagcgtgatc cagacacggt
      241 ccatgccggc gtccgccacg aggctgcggg cgacctgggg cagcgccgta tcggcgccct
      301 ccgaagcgag gacgcgcgtg atcccataga gggccgtcga ctcggcgacc cgagactcag
      361 cctcctccgc gcgatccgcg ccgatcccgg ccaggcgccc gatcaccagc gcgacgaaca
      421 ggaacagcac gaggttcagc cactcgcggg cgtccgcgac gatgagcgtg aaccggggct
      481 cgatgaacag caggttgtag atgacgaacg ccgcgatggc ggtcgcgatg ccggccgcgg
      541 tacgcaggcg ggtcgcgacg atgacgacgg cgacgaggta gatcgacgag gcatcgaggt
      601 cgccgcccgc gacggcctcg accagcgcca cggcggcggt cgcgatcgcg aggacgaggg
      661 cgatgaacag ggcgtcgcgg aacagggccg cgcgcgaggc gcgccgggtg tcggtcgtgg
      721 gcgtcggcga ggtcaccgac agatcgtagt cccgcccgtc gcggcgaatt tggtagcgtg
      781 gggccatgcc ctcccgcacg atcccgcggc gcccgggccg gccgatcggg gtcggggtcc
      841 agctgcccga ggtggagcgc gaggtccgct ggccggagct gcgcgcgatt gcgcggacgg
      901 cggagcaggc gggcttcgac tcgatctggt tcggcgacca cctcctgtat cgctacgcgt
      961 cgggcgagac gcgcggaccg tgggaggcgt ggacgacgct ggcagccttg gccgaggcga
     1021 cggagcgcgt cgcgctcggc ccgctcgtcg ccgcgaccgc gttccacgcc ccgttcatgc
     1081 tcgcgaagca ggcggcgacg gtcgacgaga tctcgggcgg ccggctcgtg ctcggcctcg
     1141 gcgcgggctg gaacgacacc gagttcgcgg cgctcggggc gccgttcgac caccggatct
     1201 cccggttcga ggaggcgttc acggtcgtgc ggacgctgct cgccgagggc gcgatcgact
     1261 tcgccggggc gtacgtccag gcacgcgacg ccgagctgct gccgcgcccg gcgcggcccg
     1321 gcggaccgct ggtcctgatc gggtcgagcg gcccgcgcat gctcggcgcg acgatgccgt
     1381 acgtggacgc atggaacgcc tggtatgcgg acacggggaa cgcccccgcg ggcgtcgccg
     1441 cgctgcgcgc gaacgtcgat gccgccgcgg ttgccgcggg acgcgagccg gacgccatcg
     1501 agcgcacggt cgccgtgctc gtggggatgc ccggcgggaa cgggcggatc atgggggaca
     1561 cggatccgcg ccaggcggtg ccgccgctca gcgggtcgcc cgaggcgatc gcggaggaat
     1621 ttcgcgggta cgcgcgcgag gggatcgggc acgtgcagct ggtcgtcgat cccatcaccg
     1681 aggcatcggt cgccgcgctt gcgccggtgt tggaggcgct cgatcgcgac tgacgcggcg
     1741 gcccctcccc ggcgtgcgtg gtgcgtgcta gcatccgccg ccagccgcgc aatcagagag
     1801 gccgtcccgt gagcgacgcc caaccgcgca cccgtacgcg atccgcgagt ggccgcgccg
     1861 aggtctcgga cctcgacaag cggatcatcg agcacctcca gcaggatggg cgccggccgt
     1921 atacccagat cgcgtcggac ctcggcgtgt ccgaggcggc cgtgcgggcg cggaccaacc
     1981 ggctgatcga gaagggcatc ctccaggtcg tgggggtcac cgacccgctc aagctcgggt
     2041 tccagcagtg ggcgatgatc ggcgtccggg tcgaggcgtc gcgcctgatc gaggtcgctg
     2101 aggagatcgc ggcgttccca gagacggact acgtggtcgt caccgcgggc agctacgaca
     2161 tcctcgtgga ggcggtcgcg gaggacaacg aggcgctgct ccagttcctg tccgaacggc
     2221 tgcgcaagat ccccggggtc cgcgagaccg agacgttcgt ctacctgcgg ctgatgaagc
     2281 agagctacca gtgggggacg cgctgagcgt cgtcccggtc gcgcgtggtg cacctgcgca
     2341 ccggatggcc cgcctcggag tggtgatttc gcgcaacggc ggtaggatgc ccgcagggcg
     2401 gcacgggggc gccgccgcac gcgggggcac gacgtgccga tcgagacgct acttgcgctg
     2461 ctcgggatcg cgcttctggc gtttctcgtc ctgatcgtcg tttcgccgac gcccatcggc
     2521 gagcggctgc gtcggacggc cgggcgaacg cgcgacgagg agttgatgcg gggcgagccg
     2581 agcgccccgg gggacctgcg gcccgccgcc ctgtaccggg tcatccgggt ctcgacctgg
     2641 gtgttcatct tcgcggcggc gacgatcgtc gcggtcaccg acctgtggtc gacccagcag
     2701 ggtccgatcc tgctcgtcct cgcggcagcc gcgatcacgc tgctcgtgat ccagcccctg
     2761 cgcggccgga tccgcgagac gctgctgctc acgatcgagg gtgcgatcgg gctcgtcctg
     2821 gccggcatgt tgatcgcgct cacgggcggg gccctcagcc ccttcgcgtt caccctggcc
     2881 ctcatcgtcg tgggcgccgc gatcgtggtg acgccggacg cggcgctcct gctgacggcg
     2941 gcggcgggag cgacctacct cgctgccatg gcattcgacg cccagacgcg atccgggccg
     3001 ccgaccgaga tcgccgtcgc gaccgcgggg atcaacctca ccgccctgtg cctcatcgcc
     3061 tacgtcggca tgtcgatcgg gcgcgagcaa cggcgcgccc gggaagacgc gaaccggcag
     3121 gcgaccgagg atccgctgac cggcctccgg acccgcccgt tcctgttcgc cgcgctggat
     3181 cgcgaggtcg atcgcagccg gcgcacgggt cgcagcttct gcctcctcat gctcgatctc
     3241 gacgacctca aggcgatcaa cgacagcatg ggccacctgc acggcgaccg ggcgctgcgc
     3301 ctcgtcggcg acgtcgtccg caacgggatc cggcgcatcg acacgggcgc ccgattcggt
     3361 ggggacgagt tcgtggtcct gctccccgag accgatccga cgggcggctg ggtcctcgcc
     3421 gagaagatcc gccagggcgt ggccgaatcg gacctcgcgg tcgacggggt cccgatcccg
     3481 acgtcggtgt cggtcggcgt ggtcacctat ccgcaggacg ggctgaccgt tcgcgcgctg
     3541 ctcgagtgcg ccgacgccgc catgtaccgc tcgaagcgcg agggccgcga ccgggtgagc
     3601 ggcgtgccgg tcatggaccc cgatccgagc ctcaccgggt cacgaagccg cccggtgtag
     3661 catccgcgcg tgaccagcaa gcgacccgga ccgggcccgt cgacgcgcgc gatccgagcc
     3721 gcgtcgcgcc aacccgtcgt gcgccagtcg cccacctcgg tcccgatcta ccagacggca
     3781 acgttctcct ccgccgatgc cgaggagctc gccgcggtgc tcaccgagga gcagcaaggc
     3841 tacgcataca gccgcatcg
//
LOCUS       DBNHXO010000090         3932 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2998260, whole genome shotgun sequence.
ACCESSION   DBNHXO010000090 DBNHXO010000000
VERSION     DBNHXO010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2998260"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="AC162A_01915"
     CDS             complement(<1..658)
                     /locus_tag="AC162A_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009621.1"
                     /translation="MTETTAPTGSPVADPLAAGRVAYGRHEWREAFDRLGEADRAGSL
                     GGEDLESLSTAAFFAAEATAGNEAKERAYRAYVAEGNAVRAAYLALDIAHEHGYAGRH
                     SMASAWLRKAEQQLDGLPESYAHGYAVLLRSEAAIVADPELALSLAQEAIDIGNRVAD
                     ADLRANAQTNLGYIKIAGGDTESGFALMEEASIAAINDELSPVVSGITCCRMISACRD
                     L"
     gene            719..922
                     /locus_tag="AC162A_01920"
     CDS             719..922
                     /locus_tag="AC162A_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009622.1"
                     /translation="MVEYGQGVGQATGVGGGSHGGGSQDLGAGAMAFMNDAVNQLNAL
                     PPTTLVIIVVLIFVGLVLVRRAF"
     gene            complement(955..3117)
                     /locus_tag="AC162A_01925"
     CDS             complement(955..3117)
                     /locus_tag="AC162A_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR5009623.1"
                     /translation="MPRLSTALSSRFQPEPVLSLQENAWLLVTLVAGPVLAVVGACIG
                     LLLSPVVSPAFVVAGVMLPVVTVIFLGVGLVRGRFLIVAAVLLNVEALAAAAMLPAGI
                     AVAIVLPMISIALVQNLLQTRPLLWASIAAAVVATVGVTLAVLVGPASVLFVGTATPV
                     TIGCFTALVAFALALDWRSTHRLRVALDAAESEIAARNAAEASLERTSSLLSAIVDGS
                     PLATQAFTLDRTVTVWNPAAERIFGWTADEIIGHPLPPAMLPDDERESSVERIRKTIE
                     GEPTRGDRVRRFTKDGREVWIEIYGSVLHGADGRPLGVAGQLADVTERVAIEAQLAHA
                     ERLEAIGRVAGGIAHDFNNTLTAIGGFAALISDETADPAAVKADAATIVDVVERSRQL
                     TRQLLAFARRSTLQPAIVDIRSAVTSVEPMLHRLLDGSVALTIRQPDDALMARVDAGQ
                     LEQALLNLAVNAGDAMVGGGTLTVCMRRVRVAAGDIPDEPDASGPFVAVAMSDTGTGM
                     TPDVIARVFEPFFTTKETGRGTGLGMAMVRDFVRQSGGSVRLESAPGTGTTVELLFPE
                     LVGARADNASGPDPVARPTGTESILLVDDEPAVAAFARRSLTDLGYEVRVARNVREAV
                     DAANGGEAIDLLISDLVLPDGTGPELAETLRRARPGARTLFACGYTVAALAARGVRYE
                     GLDVLGKPYNGVELATRVRRALDGRGFDAGGAEAPSAA"
     gene            3242..3868
                     /locus_tag="AC162A_01930"
     CDS             3242..3868
                     /locus_tag="AC162A_01930"
                     /inference="COORDINATES: protein motif:HMM:NF028169.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5009624.1"
                     /translation="MSGSSADRQATSNEHRHAGGRPRLPDTDRAIVKAVADLMAEQGV
                     RGTTINAVADRAGVARGTVYRRWPNRSAMIADAIRASRSWEPLELGDDLERNIRQQAE
                     EARTVLGEPSFQAMIPALAEMQLNGESDEVVRSAVFPRRRQMADMYERLAGDAGFRTD
                     IPPSLPNDLLVGAMLYRLLYDGRPPTTDEVQGMVDLILAGIRRPDARP"
BASE COUNT          571 a         1394 c         1433 g          534 t
ORIGIN      
        1 tcaggtcgcg gcaggccgag atcatccggc agcaggtgat gccgctgacg accggcgaca
       61 gctcgtcgtt gatcgccgcg atcgacgcct cctccatcag cgcgaacccg gactccgtgt
      121 ccccgccggc gatcttgatg tacccgaggt tcgtctgggc gttcgcgcgc aaatccgcgt
      181 cggccacgcg gttgccgatg tcgatcgcct cctgggcgag gctcagggcg agctcgggat
      241 ccgcgacgat cgccgcctcg ctgcgcagca ggaccgcgta cccgtgggcg tacgactcgg
      301 ggagcccatc gagctgctgc tccgccttgc gcagccacgc cgatgccatc gagtggcggc
      361 ccgcgtagcc gtgctcgtgg gcgatgtcga gcgcgaggta ggcggcccgc acggcgttgc
      421 cctcggcgac gtacgcgcgg tacgcgcgtt ccttcgcttc gttcccggcc gtggcctcgg
      481 ccgcgaagaa cgccgccgtc gagagcgact cgaggtcctc gccgccgagc gatccggccc
      541 ggtccgcctc gcccagccga tcgaaggctt cgcgccactc gtgccgcccg taggcgacgc
      601 gtccggcggc cagcgggtcg gccacgggac tgccggtcgg ggccgtcgtc tcggtcatcg
      661 gggggattgt gccacccgcg ggcgcagggg ccttgcgccc gggtgcgaag atgcgcgcat
      721 ggtcgagtac gggcaggggg tcggtcaggc gactggcgtg ggcggcgggt cgcacggcgg
      781 cgggtcgcag gacctcggcg ccggcgccat ggcgttcatg aacgacgccg tcaaccagct
      841 gaacgcgctg ccgcccacca cgctcgtgat catcgtggtg ctgatcttcg tcggcctggt
      901 gctcgtccgc cgcgcgttct gacactcggc ggctcggacg accccgcgca agcgctacgc
      961 ggcggacggc gcctcggccc ccccggcgtc gaagccacgc ccgtcgaggg ctcggcggac
     1021 gcgcgtcgcg agctccacgc cgttgtaggg cttgcccagc acgtccagcc cttcgtagcg
     1081 gaccccgcgg gccgcgaggg cggcgaccgt gtacccgcac gcgaacaggg tccgcgcgcc
     1141 cggtcgtgcc ctgcgcagcg tctcggcgag ctccgggccg gtgccgtcgg gcaggaccag
     1201 gtcgctgatc agcagatcga tcgcctcgcc gccgttggcc gcatccacgg cctcccggac
     1261 gttgcgggcg acccggacct cgtagccgag gtccgtgagt gagcgccgcg cgaaggcggc
     1321 gacggcgggc tcgtcgtcga cgagcaggat cgactcggtc cctgtcggcc gcgcgaccgg
     1381 atcgggtccg ctcgcgttgt cagcacgcgc tccgacgagc tcggggaaca gcagctccac
     1441 cgtcgtcccg gtgccgggcg ccgactcgag gcgcacgctg ccgcccgact ggcggacgaa
     1501 gtcgcggacc atggccatgc cgaggcccgt gccgcggccg gtctccttgg tcgtgaagaa
     1561 cggttcgaac acgcgcgcga tgacgtccgg cgtcatgccc gtcccggtat cggacatcgc
     1621 gacggcgacg aacggtcccg acgcgtcggg ctcgtcgggg atgtcgcccg cggcgacgcg
     1681 tacacgccgc atgcagacgg tgagggttcc gccgccgacc atcgcgtcgc ctgcgttgac
     1741 cgcgaggttg agcaaggcct gttcgagctg tccggcatcg acgcgcgcca tgagcgcgtc
     1801 atcgggctgg cggatggtga gcgcgaccga gccgtcaagg aggcgatgga gcatcggctc
     1861 cacggaggtc acggcggacc ggatgtcgac gatcgccggc tggagcgtcg agcgccgtgc
     1921 gaaggcgagg agttggcgcg tgagctggcg ggagcgctcg acaacgtcga cgatcgtcgc
     1981 cgcgtccgcc ttgacggccg caggatcggc cgtctcgtcg ctgatcagcg ccgcgaaccc
     2041 tccgatggcc gtgagcgtgt tgttgaagtc gtgcgcgatg cccccggcga cccgcccgat
     2101 cgcctcgagg cgctcggcat gggccagctg ggcttcgatc gcgacgcgct ccgtgacgtc
     2161 ggccagctgc ccggcgacgc ccaggggccg gccgtccgct ccgtggagca ccgagccgta
     2221 gatctcgatc cagacctcgc gcccatcttt ggtgaaccga cggacccggt cgccgcgggt
     2281 cggctcgccc tcgatggtct tgcggatccg ctcgaccgac gactcgcgct catcgtcggg
     2341 cagcatcgcg ggcggcagcg ggtgcccgat gatctcgtcc gcggtccagc cgaagatccg
     2401 ttccgccgcg gggttccaga cggtcaccgt ccggtcgagg gtgaacgcct gggtcgccag
     2461 gggcgagcca tccacgatcg cggacaggag cgagctcgtg cgctcgaggc tcgcctctgc
     2521 ggcgttgcgg gccgcgatct cggactccgc tgcgtccagc gccacccgga ggcgatgcgt
     2581 cgaccgccag tcgagcgcga gggcgaacgc gacgagcgcc gtgaagcagc cgatcgtgac
     2641 cggggtggcg gtgcccacga acagcacgct ggccgggccg acgaggaccg cgagcgtgac
     2701 gccgacggtt gcgacgaccg ccgccgcgat cgacgcccag agcagcgggc gagtctggag
     2761 caggttctgc acgagcgcga tgctgatcat cggcagcacg atcgcgacgg cgatgccggc
     2821 cggcagcatc gcggcggcgg ccagcgcctc gacgttcagc aggacggcgg cgacgatgag
     2881 gaagcggcca cggacgagcc cgaccccgag gaagatgacg gtgacgaccg gcagcatgac
     2941 gccggcgacc acgaacgccg ggctcacgac cgggctgagg agcaggccga tgcaggcgcc
     3001 gacgacggcg agcacgggtc cggcgacgag ggtgaccagg agccacgcgt tctcctgcag
     3061 cgacaggacg ggctcaggct ggaatcgcga tgacaaagcg gtgctgaggc gaggcaatca
     3121 acgggtcctt cggcgacagg accgcgctcc ggacaacccc gaccggcgac agacctgaac
     3181 cccttcaggc taccggttac gatacagagc gtcgagtatc taatatggta aagtctccga
     3241 catgtccggg tcgtccgccg atcgccaggc caccagcaac gagcatcgcc atgcgggtgg
     3301 gcggccccgt ctgccggaca cggaccgcgc gatcgtcaag gccgtcgccg acctgatggc
     3361 cgagcagggc gtgcggggaa cgacgatcaa cgcggtcgcg gatcgtgcgg gagtggcccg
     3421 cgggaccgtc taccgccgct ggcccaaccg atcggcgatg atcgccgatg cgatccgcgc
     3481 gtcgcgcagc tgggagccgc tcgagctcgg cgacgacctc gagcgcaaca tccgccagca
     3541 ggccgaggaa gcacgcaccg tgctcggcga gccgtcgttc caggcgatga tcccggcgct
     3601 tgccgagatg cagctcaacg gcgagtcgga cgaggtcgtg cgctcggccg tgttcccgcg
     3661 gcgacggcag atggccgaca tgtacgagcg cctcgcgggc gacgccggct tccggaccga
     3721 catcccgccc tcgctgccga acgacctgct cgtcggcgcg atgctctacc ggctgctcta
     3781 cgacgggcgg ccacctacga ccgacgaggt gcagggcatg gtcgacctca tcctcgccgg
     3841 aatccgtcgt cccgacgcgc ggccgtagcc gccggacctc gtgtagggtc cacgcatgcg
     3901 actcggctac gggctcctct cggcacagcg ac
//
LOCUS       DBNHXO010000091         1593 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_815717, whole genome shotgun sequence.
ACCESSION   DBNHXO010000091 DBNHXO010000000
VERSION     DBNHXO010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_815717"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /gene="cofD"
                     /locus_tag="AC162A_01935"
     CDS             complement(<1..933)
                     /gene="cofD"
                     /locus_tag="AC162A_01935"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HIR5009625.1"
                     /translation="MTAASVVALAGGVGGAKLAEGLAAHLGDRLSVVVNTGDDCERHG
                     LLVMPDHDTVLYNLAGIEQVEWGWGIEGDTHATMEQLAAYGEEDWFSLGDRDLALHVA
                     RTGRLRAGGRLTEVCLGLQGSLGIAPRILPMADEAVRTEVRVDEGWLEFQEYFVHRRQ
                     GPDVHEVRLVGVEAARPTPEVLAALEAASLIVVCPSNPIVSVGPILAIPGMRGGIDAA
                     RSRGVPVVAVSPIVGGRALKGPADRMLVSLGHDSSALGVARLYADLADVFVLDTVDAA
                     LADEVAALGLRPIVADTIMTDDASRARLAGVVLES"
     gene            complement(930..>1593)
                     /locus_tag="AC162A_01940"
     CDS             complement(930..>1593)
                     /locus_tag="AC162A_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR5009626.1"
                     /translation="TTLDHLSGGRAILGLGGAWFEREHEAYGIDFGSGFGERLDRLDE
                     AVMLVRRLLDGETVTHDGPVYPMRDALVTPRPVQVRLPIMIGGSGPKKTLRTLARYGD
                     QWNAMGELPKLAERDAILREHCATFGRDDAEIERTTTVDIVIRDTHEAGLAYYQDRLT
                     ANGEEFDADWNYFVGRPADIAQGLQPLLALGFRHVLIDMPAPYDLETLGRMGELIELL
                     NA"
BASE COUNT          226 a          599 c          558 g          210 t
ORIGIN      
        1 ggactcgagc acgacgcccg cgaggcgggc gcgggaggcg tcgtcggtca tgatcgtgtc
       61 ggcgacgatg ggccgaaggc ccagcgccgc gacctcgtcg gcgagcgccg catcgacggt
      121 gtcgaggacg aacacgtcgg ccagatccgc gtatagccta gccaccccga gcgccgacga
      181 gtcatggccc agggagacga gcatccggtc ggccgggccc ttgagcgcgc gacccccgac
      241 gatggggctt acggcgacga ccgggacgcc gcgcgaccga gcggcgtcga tccccccacg
      301 catccccggg atcgccagga tcggcccgac cgagacgatc gggttggacg ggcagacgac
      361 gatcagcgag gccgcctcga gcgccgcgag cacctccggc gtcggccggg ccgcctcgac
      421 cccgaccagc cgcacctcgt ggacgtcggg cccctggcgg cgatggacga agtactcctg
      481 gaactcgagc cacccctcgt cgacgcgcac ctcggtccgc accgcctcgt cggccatcgg
      541 caggatgcgc ggcgcgatcc ccagcgaccc ctggagtccg aggcagacct cggtcaggcg
      601 cccgcccgcg cggagccgcc ccgtccgcgc gacgtgcagc gcgagatcgc ggtcgccgag
      661 cgagaaccag tcctcctcgc cgtaggcggc gagctgctcc atcgtcgcgt gcgtgtcgcc
      721 ctcgatgccc cagccccact cgacctgttc gatcccggca aggttgtaga gcacggtgtc
      781 gtggtcgggc atcacgagca gcccgtggcg ttcgcagtcg tcgccggtgt tgacgacgac
      841 gctgagacga tccccgaggt gcgcggccag gccctcggcg agcttggcgc cgccgacccc
      901 gccggcgagc gcgacgacgg acgcggcggt cacgcgttga gcagctcgat cagctcgccc
      961 atgcgcccca gcgtctcgag gtcatacggc gcgggcatgt cgatcaggac gtggcggaag
     1021 cccagcgcga gcagcggctg caggccttgg gcgatgtcgg ccggccgccc gacgaagtag
     1081 ttccagtccg cgtcgaactc ctccccgttc gccgtcagcc ggtcctggta gtaggcgagc
     1141 cccgcctcgt gcgtgtcgcg gatcacgatg tcgacggtgg tcgtgcgctc gatctccgcg
     1201 tcgtcgcgcc cgaaggtcgc gcagtgctcg cggaggatcg cgtcgcgctc ggccagcttc
     1261 ggcagctcgc ccatggcgtt ccactggtcg ccgtaccggg ccagcgtccg gagcgtcttc
     1321 ttaggccccg acccgccgat catgatcggc agccgcacct gcaccgggcg cggggtgacg
     1381 agcgcgtcgc gcatcgggta aacgggtccg tcgtgggtca cggtctcgcc gtcgagcagc
     1441 cgccgcacga gcatcaccgc ctcgtccagg cggtccagcc gctccccgaa cccgctgccg
     1501 aagtcgatcc cgtaggcctc gtgctcccgc tcgaaccagg ccccgccgag accgaggatc
     1561 gcccgcccac cggagaggtg gtcgagcgtc gtc
//
LOCUS       DBNHXO010000092         3301 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_815747, whole genome shotgun sequence.
ACCESSION   DBNHXO010000092 DBNHXO010000000
VERSION     DBNHXO010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_815747"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..807)
                     /locus_tag="AC162A_01945"
     CDS             complement(55..807)
                     /locus_tag="AC162A_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009627.1"
                     /translation="MPLEFLKRRGGDADQPAEGSQSKAVPGLPEEVVAQEYQLKLYYA
                     GKSSEGVRLKAGPRALAELPSMLAGIARSDVEVVEPLPLEYSQAVPQIVRPSEAMQWL
                     NAHHQFTPITRHALVVLETIDAVDLAFDTFACGLLDGETDTSGYPEYNAVVGGVASHW
                     DEATGDMIVRSVVGWGGRGVRGDTDRIASRILGGLLTNILANQFAMGLTAVERPVPAA
                     GRGGLVCAHCGFASGHERAFYCPKCGMRLLRG"
     gene            complement(892..>3301)
                     /locus_tag="AC162A_01950"
     CDS             complement(892..>3301)
                     /locus_tag="AC162A_01950"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR5009628.1"
                     /translation="KEGESRASLGRERYLERMRAFIEATRTVILDQQRRVGGSCDWGR
                     LRFTMDEVSARAVRVAFLRLHEKGLAYRTEALVNWCPGCRTSVSDLEVLPTPETGTLW
                     SVRYHLIDEATGRPDPDATLTVATTRPETILGDTAVAVHPDDPRYAALVGRRVRIPFV
                     DRDVPVIADAVVDPAFGTGAVKITPAHDHDDNETGKRHGLAMPTILDDEARVTGTGTP
                     YDGLDRYEARQRIVADLAAQGDLAGERAHEMVIGRCQRSNDVVEPRLKTQWFVRTAPL
                     AAAALEATRSGRTTILPARLEKTWEHWMTNIRDWNVSRQLWWGHRIPAWYCPDGHATV
                     SAEPEGPAACATCGRPAAELVQDPDIFDTWFSSGLWPFSTLGWPEPTDDLARYYPGSV
                     METGYDILFFWVARMMMLGIELTGEAPFHTVYLSGLIRDPYGQKLSKTKGNTVDPLDT
                     IEVLGADALRFALVYGATPGNDQRFGQAKVENARNFANKLWNATRFVLGARPSTVPDG
                     SGRRLPDPAHLGPADRWVLSRAAATIAAVDHAMADYNFGEVSRLLYEAIWSEYCDWGL
                     ELAKVRLADPDRTDAEREATWWALVDALDVYLRLLHPVMPFITERLWQGLPHTDDDPG
                     LLIVARWPGARTRDEAAEAEVGALVELVRAIRNARADAKVEPAAWLPLDVYVEPSLGH
                     ALEGLRPAIERLARARPLRRHLTPEDLHGTASATGGLTVIAGPAEAIIGQPAGEAGAA
                     GADRARLEKELADTERLLAAARARLANDAFTSKAPPAIVEGARASEAELADQAERLRD
                     RLAR"
BASE COUNT          448 a         1169 c         1205 g          479 t
ORIGIN      
        1 ggctgcacgc ggtgcagagg tactcgtaga tcggcatcag gggctcggcg cggctcagcc
       61 gcggagcagc cgcatcccgc acttggggca gtagaacgct cgctcgtgcc ccgatgcgaa
      121 cccgcagtgg gcacacacga gaccgccccg acccgcggcc ggcacgggcc gttcgacggc
      181 ggtcagtccc atcgcgaact ggttcgcgag gatgttcgtg aggagcccgc ccaggattcg
      241 gctcgcgatc cggtcggtgt cgccgcgaac cccgcggccc ccccagccga ccacgctgcg
      301 cacgatcatg tcgccggtcg cctcgtccca gtgcgaggcc acgccaccga cgaccgcgtt
      361 gtactcgggg tagccggacg tgtccgtctc gccatcgagc agcccgcagg cgaacgtgtc
      421 gaacgcgagg tcgaccgcgt cgatcgtctc gagcacgacg agggcgtggc gggtgatcgg
      481 ggtgaactgg tggtgggcgt tcagccactg catcgcctcg gatggccgga cgatctgggg
      541 cacagcctgg gagtactcga gcggcagggg ctcgacgacc tcgacgtcgc tgcgggcgat
      601 gccggcgagc atcgacggga gctccgccag ggcgcgcggt ccggccttga ggcggacgcc
      661 ctcgctgctc ttgcccgcgt aatagagctt gagctggtac tcctgcgcca cgacctcctc
      721 ggggaggccc gggacggctt tggactgtga gccttcggcg ggttgatcag cgtcaccccc
      781 gcgacgcttc aggaactcga ggggcacgac tcctccggac gcggccagcg gacaggatgg
      841 gcagatcgta gcagcgcgcg cggggccggg gcggcgtccc gggcgccgcg atcagcgggc
      901 gaggcggtca cgcagccgtt cggcctggtc cgcgagctcc gcttcgctgg ctcgggcgcc
      961 ctcgacgatc gccggcggcg ccttggaggt gaaggcgtcg ttggcgaggc gggcccgtgc
     1021 ggccgccagc aggcgctcgg tgtccgcgag ctccttctcg agccgggcgc ggtcggcgcc
     1081 ggcggcgccc gcctcccccg ccggctggcc gatgatcgcc tcggcgggtc ccgcgatgac
     1141 ggtgagtccg cccgtcgcgc tggccgtgcc gtggaggtcc tcgggcgtga ggtgacggcg
     1201 aagcgggcgc gcccgcgcca ggcgctcgat cgccggacga agcccctcga gggcgtggcc
     1261 cagcgaaggc tcgacgtaga cgtcgagcgg gagccatgcc gccggctcga ccttcgcgtc
     1321 ggcacgcgcg ttgcggatgg cccgcacgag ctccacgagc gcgccgacct ccgcctccgc
     1381 cgcctcgtcc cgcgtccgtg cgccgggcca gcgcgcgacg atcagcaggc ccgggtcgtc
     1441 gtcggtgtgc ggaagcccct gccacagccg ctccgtgatg aacggcatga ccggatggag
     1501 caaccgcagg tagacgtcga gggcgtcgac cagcgcccac cacgtcgcct cccgctcggc
     1561 gtcggtccga tcgggatcgg ctagccggac ctttgcgagc tcgaggcccc aatcgcagta
     1621 ctcgctccag atcgcctcgt acaggagacg cgacacctcg ccgaagttgt agtcggccat
     1681 cgcgtggtcg acggcggcga tcgtcgccgc cgcgcgcgac aggacccagc gatctgccgg
     1741 cccgaggtgc gcgggatccg gcaggcgacg tccgctgcca tccgggaccg tcgacggacg
     1801 cgcaccgagg acgaaccgcg tggcattcca cagcttgttg gcgaagttgc gggcgttctc
     1861 gaccttggcc tggccgaacc gctggtcgtt gccgggggtc gcgccgtaca cgagggcaaa
     1921 gcggagggcg tcggcaccca gcacctcgat cgtatccagc gggtcgaccg tgttgccctt
     1981 cgtcttcgac agcttctggc cgtacgggtc acggatcagg cccgacaggt agaccgtgtg
     2041 gaagggagcc tcgccagtga gctcgatgcc cagcatcatc atccgtgcga cccagaagaa
     2101 caggatgtcg taacccgtct ccatgacgga gcccgggtag tagcgagcga ggtcgtcggt
     2161 cggctccggc cagccgaggg tcgagaacgg ccacaggccc gagctgaacc aggtgtcgaa
     2221 gatgtccggg tcctggacga gctcggccgc cggtcgcccg caggtcgcgc aggctgccgg
     2281 accctcgggc tccgcgctga ccgtggcgtg gccgtcgggg cagtaccagg ccgggatccg
     2341 gtggccccac cacagctggc gtgacacgtt ccagtcccgg atgttggtca tccagtgctc
     2401 ccaggtcttc tcgagcctgg cgggcaggat cgtcgtccgg ccggagcgcg tggcctcgag
     2461 cgccgcggcg gcgagcggtg ccgtccgcac gaaccactgc gtcttgaggc gcggctcgac
     2521 gacgtcgttg ctgcgctggc agcggccgat caccatctcg tgggcgcgct cgcccgccag
     2581 gtcgccctgc gccgcaaggt ccgcgacgat ccgctgccgg gcctcgtagc ggtcgagacc
     2641 gtcgtacggc gtgccggtgc cggtgacccg cgcctcgtcg tcgaggatcg tcggcatggc
     2701 cagcccgtgg cgcttgccgg tctcgttgtc gtcgtgatcg tgggctggcg tgatcttgac
     2761 agcaccggtc ccgaacgccg ggtccacgac cgcgtcggcg atgaccggca cgtcgcgatc
     2821 gacgaacggg atacgcacgc gccggccgac gagggccgca tagcgcgggt cgtctggatg
     2881 cacggccacg gcggtgtcgc cgaggatcgt ctccgggcgg gtcgtggcca cggtgagggt
     2941 cgcgtccgga tcgggccggc cggtcgcctc gtcgatcagg tggtagcgga cgctccacag
     3001 ggtcccggtc tcgggcgtcg ggagcacctc gaggtcgctg acgctcgtcc gacagcccgg
     3061 acaccagttc acgagcgctt ccgtgcggta cgcgagaccc ttctcgtgca gccgcaggaa
     3121 cgccacgcgg acggcccgcg ccgacacttc gtccatcgtg aaccgcagcc ggccccagtc
     3181 gcagctgccg ccgacccgcc gttgctggtc gaggatcacc gtccgggtgg cctcgatgaa
     3241 cgcgcgcatg cgctcgaggt agcgctcgcg acccagggac gcgcggctct ccccttcctt
     3301 c
//
LOCUS       DBNHXO010000093         1524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4190010, whole genome shotgun sequence.
ACCESSION   DBNHXO010000093 DBNHXO010000000
VERSION     DBNHXO010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4190010"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="AC162A_01955"
     CDS             <1..1249
                     /locus_tag="AC162A_01955"
                     /inference="COORDINATES: protein motif:HMM:NF027510.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HIR5009629.1"
                     /translation="ALAEDGVLTVGAAGVLANDTDFDLDPMTAAVVAGPANGTLTLDA
                     SGSFTYTPNANFNGTDSFTYQANDGFGNSNVATVTITVTPVNDAPAIGANGVAVTDGG
                     TVGISTGNLSATDVDDVATSLVFTVGATNGRFELVSAPGVAITSFTQAQVLAGEVRFV
                     HDGSNAPPAITVYVTDGVTNAGPFAMNVSFTGSGFVATPPTGGGGPGGSTEIPPLLAP
                     SASQRDPGDLQHAGPSTFLRSPTGAPDDGGGDGEEEVVAPVPSVPGGTLLRDKVAAPV
                     TGMPTVRSDSETIDTKPLRSEIEVEPLRAEMQVLPMRRDAFDNPEDEERKTIEVIMSS
                     VKVTGLAFSVGAVWWAVRAAGLMASLLASSPAWRHVDPLPVLGRDDEEEEVWDEAADE
                     EDKDRKDDEHRAAWVLEEREAGS"
BASE COUNT          219 a          565 c          545 g          195 t
ORIGIN      
        1 cgcgctcgcc gaggacggcg tcctgaccgt cggcgccgcg ggcgtgctcg cgaacgacac
       61 cgacttcgac ctcgacccga tgaccgcggc cgtggtcgcc ggccccgcga acggcacgct
      121 gacgctcgac gccagcggca gcttcaccta caccccgaac gcgaacttca acgggacgga
      181 cagcttcacc taccaggcca acgacgggtt cggcaactcg aacgtcgcca cggtgacgat
      241 cacggtcacc ccggtcaacg acgcgccggc gatcggggcg aacggcgtcg ccgtgaccga
      301 cggcggcacg gtcgggatct ccaccggcaa cctcagcgcg accgacgtcg acgacgtcgc
      361 cacgagtctc gtgttcaccg tcggtgcgac caacggccgc ttcgagctcg tctcggcgcc
      421 cggcgtggcg atcacgagct tcacgcaggc gcaggtcctc gccggcgagg tgcgcttcgt
      481 gcacgacggc tcgaacgccc cgccggcgat caccgtctac gtgaccgacg gcgtgaccaa
      541 cgccgggccg ttcgcaatga acgtctcctt caccgggagc ggcttcgtgg cgaccccgcc
      601 gaccggcggc ggcggccccg gcggctcgac cgagatcccg ccgctgctgg cgccgtccgc
      661 gtcgcagcgg gacccgggcg atctccagca cgcgggcccg tccaccttcc tgcgctcgcc
      721 gacgggcgcg cccgacgacg gcggcggcga cggcgaggaa gaggtcgtcg cgccggtccc
      781 gagcgtcccg ggcggcacgc tgctccgcga caaggtcgcc gcgccggtga cgggcatgcc
      841 gacggtgcgt tcggatagcg agaccatcga caccaaaccg ctgcgctcgg agatcgaggt
      901 cgagccgctt cgcgccgaga tgcaggtgct gccgatgcgc cgcgacgcgt tcgacaaccc
      961 ggaggacgag gagcgcaaga cgatcgaggt gatcatgagc tcggtcaagg tcaccggcct
     1021 cgcgttctcg gtcggcgcgg tctggtgggc ggtgcgcgcc gccggcctca tggcgagcct
     1081 gctcgcctcc tcgcccgcct ggcgccacgt cgatccgctg ccggtgctcg gccgcgacga
     1141 cgaggaggaa gaggtctggg acgaggccgc cgacgaagag gacaaggacc gcaaggacga
     1201 cgagcaccgc gccgcgtggg tcctggagga gcgggaagcg gggtcctgac cggcggcccg
     1261 cgaccgggct gacggtcgtt cggcaagacg ctgtcgtgcg ggtgtcatgg atccgctccg
     1321 gcggcctccg ccggcggctt tccgggcggg cgaaccggaa atagttcacg cggccgcttg
     1381 cgtcgccgcc ggcggcggac cggcgatcgc ctgccgtttg tggcatctgt cgcccgccgc
     1441 gacggccccc ccgtgagaac tgcaccctcc gcccgcggcg ggctcgcctc ttgcgattcc
     1501 cttcccgcag aagcagttgg gggc
//
LOCUS       DBNHXO010000094         1514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_23306, whole genome shotgun sequence.
ACCESSION   DBNHXO010000094 DBNHXO010000000
VERSION     DBNHXO010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_23306"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..862
                     /locus_tag="AC162A_01960"
     CDS             77..862
                     /locus_tag="AC162A_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009630.1"
                     /translation="MDEREQESFDEPAHVIVSGTAPRRLYAVIVVLLAVIVVLAIRPW
                     GDDARAPAPPPEGQASPGVDSDASGEPGVSAVTAPPPVAGAAGMMETCGTPSGWRAAT
                     LQDWAGRDSPIRSWIAIVPVEATSPLDPAIPFVPVATGIVTAIGYCAPLDEAARPPAV
                     ARASLWAIGDGRQTALSLIPLEPDSPDSLGGLWSRPGEITGGEASRGVWPPGRYVVEI
                     ASPGGEYRRWLGIEIADLALLRASPSAPLPSPSAPSSSGSPAP"
     gene            complement(872..1300)
                     /locus_tag="AC162A_01965"
     CDS             complement(872..1300)
                     /locus_tag="AC162A_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009631.1"
                     /translation="MHRRPLGNGRRLALIGAIVLVIGCLLPWYSVGGDGGLPEIVLRA
                     FDTTGIASFLAALATLALIALPYAAGERPVAIDRGVSFAILGAIAVAGVVLFPLIYPG
                     ILEALEGLLPASAYGFWISALGAIILARAAYDIWLEPPRR"
     gene            complement(1300..>1514)
                     /locus_tag="AC162A_01970"
     CDS             complement(1300..>1514)
                     /locus_tag="AC162A_01970"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043174 - nucleoside salvage [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR5009632.1"
                     /translation="RYLRGKNPASLRICTLLDKPARRLVDIPVDYVGFEIPDQFVVGY
                     GLDYGERYRNLRFVGVLRPEVYTEGD"
BASE COUNT          217 a          548 c          560 g          189 t
ORIGIN      
        1 cgagctgagc accaacgaga cggtccgcaa ggcctacctc ggggaggact gacgccgagc
       61 caccggcgtc cccgccgtgg acgagcgcga acaggaatcg ttcgacgagc cggcgcacgt
      121 catcgtgtcc ggcacggccc cgcggcgact ctacgcggtg atcgtcgtcc tcctggccgt
      181 catcgtcgtg ctggcgatcc ggccatgggg cgacgacgcg cgggctcccg ctccaccgcc
      241 cgagggtcag gccagcccgg gagtggacag cgacgcgtcg ggcgaacccg gggtgtcggc
      301 cgtgacggcg ccacccccgg tcgccggggc ggccggcatg atggagacgt gcgggacgcc
      361 gagcggatgg cgggcggcca cgctgcagga ctgggccggg cgggactcgc cgatccgctc
      421 ctggatcgcg atcgtgcccg tggaggcgac gagcccgctg gacccggcca ttccgttcgt
      481 gccggtggcg accggcatcg tgacggcgat cggctactgc gcaccgctcg acgaggcggc
      541 tcgtccgccc gcggtcgcac gcgcgtccct gtgggcgatc ggtgacggcc ggcagacggc
      601 gctgtcgctg atcccgctcg agcccgacag cccggactcc ctcggcggcc tctggtcgcg
      661 gcccggcgag atcaccggcg gcgaggccag tcgcggcgtc tggccgccag gtcgctacgt
      721 cgtcgagatc gcctcacccg gcggcgagta ccgccgctgg ttgggcatcg agatcgcgga
      781 cctcgcgctg ctccgcgcgt cgccgtcggc gccgctcccg tcgccatcgg ccccgtcgtc
      841 gtccgggtcg ccggccccct gagctccacc cctagcggcg cggtggctcc agccagatgt
      901 cgtacgccgc ccgggccagg atgatcgcgc ccagcgccga gatccagaac ccgtacgcgc
      961 tggcggggag cagtccctcg agcgcctcga ggatgccggg gtagatgagc gggaacagca
     1021 cgacgccggc cacggcgatc gccccgagga tcgcaaacga gacgccgcgg tcgatcgcga
     1081 caggccgctc accggccgcg tagggcaggg cgatcagggc gagcgtcgcg agcgcggcga
     1141 ggaacgatgc gatcccggtc gtgtcgaacg cccggaggac gatctcgggc aggccgccgt
     1201 cgccgccgac cgaataccac ggcaggaggc agccgatgac gaggacgatg gcgccgatca
     1261 gcgcgagccg gcgtccgttg ccgagcggtc gtcggtgcat cagtcgcctt cggtgtacac
     1321 ctcggggcgc aggacgccca cgaagcgcag gttccggtat cgctcaccgt agtccaggcc
     1381 atagccgacc acgaactggt ccgggatctc gaaccccacg tagtcgacgg ggatgtcgac
     1441 caggcggcgg gccggcttgt ccagcagcgt gcagatccgc agcgacgccg ggttcttccc
     1501 acgcaggtag cgga
//
LOCUS       DBNHXO010000095         2027 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_222474, whole genome shotgun sequence.
ACCESSION   DBNHXO010000095 DBNHXO010000000
VERSION     DBNHXO010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_222474"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="AC162A_01975"
     CDS             complement(<1..429)
                     /locus_tag="AC162A_01975"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR5009633.1"
                     /translation="MVRRGATCGDGTNPRGVQGAGLCLTPTIPVTPQPSEIGPMRIGF
                     IGLGRMGANMVRRLLRDEHEIVAYNRTPEKTKEIEGEGAIGAFSIAELVAKLEKPRAV
                     WVMVPAGDATEAQIQELLEHLEPGDTIIDGGNTNFHDDVRR"
     gene            complement(640..1611)
                     /locus_tag="AC162A_01980"
     CDS             complement(640..1611)
                     /locus_tag="AC162A_01980"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HIR5009634.1"
                     /translation="MAQFDLPLDELREYRPPRDEPADFDAFWSETLAESRAVRAPAVA
                     VDVDSHLETIRAADVTFSGYAGQPIKAWLLWPAGRTGPLPAIVEYIGYGGGRGRPVDW
                     LVYASAGYAHLVMDTRGQGAEWQSGDTSDIENTGTGPQVPGVMTRGILDPATYYYRRL
                     MTDAVLAVDAARDLPIVDGQRVMVAGQSQGGGLALIAATLARDVTAALIDVPFLCHYR
                     RAIEVTDEAPYSEIARFLRSHRLEEERVFRTLSYFDGRNFAARARIPALFSVGLEDKI
                     TPPSTVFSAYNEYAGPKEIRVWPFNGHEAPEDDHILERLDFAARHLR"
     gene            complement(1644..>2027)
                     /locus_tag="AC162A_01985"
     CDS             complement(1644..>2027)
                     /locus_tag="AC162A_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009635.1"
                     /translation="IGLVGLVGFSIALVDDVLEARLPHPRRGIRRSTIAYLIVTLLGL
                     VVILPVVVAILLGGPEYNAPIRAGLALAVTGACTLAWWVLRVRGGETPPRALRLIGSG
                     SLLAAVILGFVTSLPQVTGALGLPR"
BASE COUNT          311 a          696 c          729 g          291 t
ORIGIN      
        1 gcggcggaca tcgtcgtgga agttggtgtt gccgccgtcg atgatggtgt cgccgggctc
       61 gaggtgctcg agcagctcct ggatctgggc ctcggtggcg tcgccggccg ggaccatgac
      121 ccacaccgcg cgcggcttct cgagcttggc gaccagctcc gcgatcgaga aggcgccgat
      181 cgcgccctcg ccttcgatct ccttcgtctt ctcgggggtt cggttgtagg cgacgatctc
      241 gtgctcgtcg cgcagcaggc ggcgaaccat gttcgcgccc atccgcccga gtccgatgaa
      301 gccgatgcgc attgggccga tctccgaggg ctggggagtg accgggatgg ttggggtcaa
      361 gcataggccc gccccttgaa cgccgcgagg gttcgttccg tcgccacaag tggctcctct
      421 tcggaccaac cgaacatcgg acgcccaatt gccgtcggat ccgtcgtcct gcgacggcgc
      481 cccgacacat caggcgctcg gtcacgctcg catacgtcgc cggggcgact tgctctcctt
      541 cgattcgacc tcgaatcaag ggcgaattcg cccagggacc cgatcctgtt cggatagacc
      601 gaaaaggcgc ccgtcccggc gccctgttgc cttcggcgtt cagcgcaggt ggcgcgcggc
      661 gaagtcgagg cgctcgagga tgtggtcgtc ctccggcgcc tcgtggccgt tgaacggcca
      721 gacccggatc tccttcgggc ccgcgtactc gttgtaggcg gagaacaccg tcgacggcgg
      781 cgtgatcttg tcctcgaggc ccaccgagaa cagggccggg atgcgggccc gggcggcgaa
      841 gttgcggccg tcgaagtagg acagggtgcg gaagacccgc tcctcctcga gtcgatggct
      901 gcgcaggaat cgggcgatct cgctgtacgg cgcctcgtcg gtgacctcga tggcccgccg
      961 gtagtggcac aggaacggga cgtcgatcag cgccgcggtc acgtcccggg ccagcgtcgc
     1021 cgcgatgagc gcgagcccac ccccctggct ttgcccggcg accatgacgc gctggccgtc
     1081 gacgatcggc aggtcgcgcg cggcgtccac ggcgagcacg gcgtcggtca tcagacgccg
     1141 gtagtagtag gtcgccgggt caaggatgcc gcgcgtcatc acgcccggga cctgggggcc
     1201 cgtgcccgtg ttctcgatgt cggaggtgtc acccgactgc cactcggcgc cctgcccccg
     1261 ggtgtccatc acgaggtgcg cgtagccggc cgaggcatag acgagccagt cgaccggccg
     1321 gccgcgaccc ccgccgtagc cgatgtactc gacgatggcc ggcagcgggc ccgtccgccc
     1381 ggcgggccac agcagccagg ccttgatcgg ctgcccggcg tagcccgaga acgtgacgtc
     1441 ggccgcgcgg atcgtctcga ggtgcgaatc gacgtcgacg gccaccgcgg gagcgcggac
     1501 ggcgcggctc tccgcgaggg tctccgacca gaaggcatcg aagtccgcgg gctcgtcgcg
     1561 cgggggccgg tactcgcgaa gctcatcgag gggcaggtcg aactgggcca tgggcggatc
     1621 gtagccggat cagcgcgccg aattcagcgc ggaaggccaa gtgcgccggt gacctggggc
     1681 aggctcgtca cgaagccgag gatcacggcg gccagcagcg aacccgaccc gatcagccgc
     1741 agcgcccggg gcggggtctc gccaccacgc acgcgaagca cccaccaggc gagcgtgcac
     1801 gcgcccgtga ccgccagcgc caagccggcc cggatcggcg cgttgtactc ggggccgccc
     1861 agcaggatgg caacgacgac ggggaggatc acgacgaggc ccagcagcgt cacgatcagg
     1921 taggcgatgg tcgagcgcct gatcccgcga cgcggatgcg ggagccgcgc ctcgaggacg
     1981 tcgtcgacga gcgcgatcga gaagccgacg aggcccacga gcccgat
//
LOCUS       DBNHXO010000096         1585 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1413077, whole genome shotgun sequence.
ACCESSION   DBNHXO010000096 DBNHXO010000000
VERSION     DBNHXO010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1413077"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="AC162A_01990"
     CDS             <1..446
                     /locus_tag="AC162A_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009636.1"
                     /translation="IVTALGQSYLNALLAISIVTIPVYARLVRSSVLAAREADYVTAS
                     RALGESGFGILRRRIMPNAITPLIVQGTLGIGGAVLEIAALAFIGVVGIGAAEWGSMI
                     SLERNQLFNAPHVILVPGIAITLTVLAFNLLGDGLRDALDPRLNR"
     gene            523..>1585
                     /locus_tag="AC162A_01995"
     CDS             523..>1585
                     /locus_tag="AC162A_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5009637.1"
                     /translation="MAPAAALRAKRGTRGDLLLDVRGLQTSFHTRDGVVRAVDGIDFH
                     VNRGEIMGLVGESGCGKSVTSLSIMQLVGPPGRIEGGQILFDGEDLLTYSPDRMRKIR
                     GNRISMIFQQPQSSLNPVWEVGAQISEVLVLHRNMGKKAAQSRSIELLKMVGIPDPER
                     RSKAYPHEMSGGMAQRVMIAMALACEPELLIADEPTTALDVTIQAQILDLMRALREET
                     GTAIILITHDLGVVAEMCDRVAVMYAGEIVEQTDTVTLFRKPLHPYTRGLIASIPVVG
                     KVQDELSVIPGNVPNLIDLPPACRFAPRCQARIEQDVALSTEVHPELLAYGTGHDVRC
                     WLYHDATGAEKAEPVLAAQG"
BASE COUNT          250 a          577 c          533 g          225 t
ORIGIN      
        1 ccatcgtcac cgccctcggg cagagctacc tcaacgccct gctggcgatc agcatcgtca
       61 cgatccccgt ctacgcgcgc ctcgtgcgct cgtcggtcct ggccgcgcgc gaggccgatt
      121 acgtcacggc atcgcgcgcc ctcggcgagt cagggttcgg catcctccgc cgccggatca
      181 tgcccaacgc catcacgccg ctgatcgtcc aggggacgct cgggatcggc ggcgcggtgc
      241 tcgagatcgc ggccctcgcg ttcatcggcg tagtcgggat cggcgcggcg gagtggggct
      301 cgatgatctc gctcgagcgc aaccagctgt tcaacgcgcc gcacgtgatc ctcgtcccgg
      361 gcatcgcgat cacgctcacg gtcctcgcct tcaacctgct cggcgacggc ctgcgcgacg
      421 ccctcgaccc gcggctcaac cggtgagccg gtgagcgacg acaccgggag cggcgagatc
      481 caggccctcg ggaccaggct cgacgagacc gtcatcgagg agatcgcgcc cgccgccgcg
      541 ctccgggcca agcggggcac gaggggcgac ctcctgctgg acgtgcgcgg cctccagacg
      601 tcgttccaca cccgggacgg cgtcgtccgg gccgtcgacg gcatcgactt ccacgtcaac
      661 cgcggcgaga tcatgggcct cgtcggcgag tcgggctgcg gcaagagcgt gaccagcctc
      721 tcgatcatgc agctggtcgg gccgcccgga cggatcgagg gcgggcagat cctgttcgac
      781 ggcgaggacc tgctgacgta cagccctgac cggatgcgca agatccgcgg caaccggatc
      841 agcatgatct tccagcagcc gcagtcgtca ctgaaccccg tgtgggaggt gggcgcccag
      901 atctccgagg tgctcgtgct ccaccggaac atgggcaaga aggccgccca gagccgctcg
      961 atcgagctcc tcaagatggt cggcatcccg gacccggagc ggcgttcgaa ggcctatccc
     1021 cacgagatgt cgggcggcat ggcccaacgc gtgatgatcg cgatggcgct cgcgtgcgag
     1081 cccgagctgc tgatcgcgga cgagccgacg accgcgctcg acgtgacgat ccaggcgcag
     1141 atcctcgacc tgatgcgcgc cctccgcgag gagacgggca ccgcgatcat cctgatcacc
     1201 cacgacctcg gcgtggtcgc cgagatgtgc gaccgggtcg ccgtgatgta cgcgggcgag
     1261 atcgtcgaac agaccgacac ggtgaccctg ttccgcaagc cgctgcaccc gtacacgcgc
     1321 ggcctgatcg cgagcatccc ggtcgtgggc aaggtgcagg acgagctgtc ggtcatcccg
     1381 ggcaacgtgc ccaacctcat cgacctcccg ccggcctgcc ggttcgcacc ccgctgccag
     1441 gcccggatcg agcaggacgt cgcgctgtcg accgaggtgc atcccgagct gctcgcctac
     1501 ggcacggggc acgacgtccg ctgctggctg taccacgacg cgacgggtgc cgagaaggcc
     1561 gagcccgtcc tcgccgccca gggca
//
LOCUS       DBNHXO010000097        11052 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3992275, whole genome shotgun sequence.
ACCESSION   DBNHXO010000097 DBNHXO010000000
VERSION     DBNHXO010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 11052)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11052)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11052
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3992275"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="AC162A_02000"
     CDS             complement(<1..790)
                     /locus_tag="AC162A_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009638.1"
                     /translation="MSLTPRILARPRTTARSGLRRAIASTVTAAGFGLVVAACGSTIP
                     PTWTMPPAAAASPASPSPSPAPSASPTPASSSPAPSPSSEPSPVASPSIAPSPEPSAS
                     PGAAGWSFVKKEACPESRFECVTLAVPSDHNTAGSPTWDVTFAIQRASGKRVGTYVVI
                     TGGPGSSGISVADSYSDYYPASVAEHMDIVFIDQRGIGLSRPFQCPDATATYYDSDAR
                     PQDPAQGTAAGAAADAYVDACLTEAGVPEADLPLYSTEQAIEDLE"
     gene            complement(807..1973)
                     /locus_tag="AC162A_02005"
     CDS             complement(807..1973)
                     /locus_tag="AC162A_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011127590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HIR5009639.1"
                     /translation="MTDTLANRRIFRSKLDTIRQTYGFEDVSLAPGTDTIDPADVVLA
                     QTFCGIELGVPVLAAAMDAVVDVKLSGELARLGGLAVLNLEGVQTRYDDPSEVLERIA
                     TAPDDAVHDVLAEAYAPPIREELIARRIDELHAAGSKAAVSATPGVARRFGPFCAEHG
                     ADLFLVQSQVSSARHLATGYEPLALEDFTRFMPIPVAVGNTTNAQAAYLLMEQGAAAI
                     FVGVGPGAACTTREVLGIGIPQVTAVADVAAARDQYHAETGTYVPVVADGGMKRGGDL
                     AKAFAAGADIVMLGSPLARAAEAPGRGTNWGMAAPSPVLPRGTRIKVGTVGSLDQILN
                     GPSHVTDGTQNLFGALRQSMAALGARTIADMHDVEIVYAPAVATEGKSWQQRGR"
     gene            2100..2969
                     /gene="ppk2"
                     /locus_tag="AC162A_02010"
     CDS             2100..2969
                     /gene="ppk2"
                     /locus_tag="AC162A_02010"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HIR5009640.1"
                     /translation="MAKGKAKADTGEGAVADPTADSYGHPSAPAAQPLSAKAYERELA
                     RLQLELSKLQEWVHARGLKVVVLFEGRDAAGKGGAIKTLTLALNPRIARVVALPAPTE
                     RERTQWYFQRYAAHLPAAGEMVLFDRSWYNRAGVERVMGFATADEIEEFFRSCPEFER
                     MLVRSGIVLIKYWFSVSDAEQERRFEARIKDPTKRWKLSPMDLQSRARWVEYSIAKDE
                     MFRHTDIKQAPWWVVNADDKRRARLNMIDHLLSQVPYQDLTPEQIVLPPREPDRGYVR
                     PPLDEQSFVPERY"
     gene            complement(2963..3499)
                     /locus_tag="AC162A_02015"
     CDS             complement(2963..3499)
                     /locus_tag="AC162A_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR5009641.1"
                     /translation="MSLRPDLIECWLFRVAPAAGAADTRLDADRLEILLIRRSASRIF
                     PGLWQCVTGGVEPGERVPMAAIREVEEETALRGADVEAWYDLDQVAPFFDEGVDGMVV
                     SAIFAARIRPDAIARLSREHDGLRWVPAAEAPALAIWPSYAESVRRIRDLLLEPGLAR
                     WFELDGDGRRLARRPLGQ"
     gene            3845..6499
                     /locus_tag="AC162A_02020"
     CDS             3845..6499
                     /locus_tag="AC162A_02020"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR5009642.1"
                     /translation="MSAGAAILLAATSIIAAPVARIVPPATAQAAGSFADSHFTEDQV
                     FTGLTWPTTVRFASDGTAFVAEKRGVIWEYDTLSTPNANRVKVLDIQTEVYDFWDRGL
                     LSIALDPDFLNGRNYIYVYYDYNAPPGQTAPYWAEPCPNPPGATTDGCVVTSKVDRYT
                     VNLNTYAASNRVNLIGDGPGGTGSEWCQQFPSHAGGALAFDSDGMLLVSGGDGASFGG
                     MDWGQLGGSSGSPTPKNPCGDPVPGFVVATAQGGMLRSQDVRTQADPTGLDGTIARID
                     PDTGNAPNDNPLKDFGGADQNTKRIVATGFRNPFRMTFRPGHDDLYVGDVGNETWEEV
                     DRWTMSGSTPTNIPNYGWPCYEGNNHQAAFENLGTNMCDDLYDDGQNAPLYTYTHEGS
                     LTPKGPCFPGAADQTSSITGLAFYEGATGSSVDYPNKYTGALFFVDYSRDCLGAILPQ
                     GNGIPDSSKVEQIASGIANPVDLVTGPGGDLYYVDHNGGRVVRVKYVSSPIARATAEP
                     EGGVAPITIHLDGSTSSEPDPDFAIDDWDWDFFNDGSYDASGEEVDWQIDTEGEYQVK
                     LRVHSTSGLTDTVTLTVDTSNAAPVPVINAPADCDAPGCWSVGTDISVSGVASDAEDG
                     NLSASHFDWSVILHHCPAGCHTHTILTKSNTKSFSFDAPDHEYPSYLEIRLTATDDDD
                     ISSSTSVELQPRTSTVQLASSPSGVPLSAGNETEPAPWTATFIRKGSATISGPLTRTI
                     NGKRYRFSTWNDSHTRVRNITVSSSKNLTATYVPDAPDSCAGATTTSPNDTWLHDRQS
                     GNSDKDWFRFRLASKRDVVIKLKDLPVNGTLRLYKSCGNQVEESSKPGTRSEKISRTI
                     AKGTWYVRVSSPGNGWSSKQYALRFKTD"
     gene            complement(6509..7441)
                     /locus_tag="AC162A_02025"
     CDS             complement(6509..7441)
                     /locus_tag="AC162A_02025"
                     /inference="COORDINATES: protein motif:HMM:NF013761.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HIR5009643.1"
                     /translation="MNQLMRKVFLWFARNTWLRERLPQLPFMQKAVRKFMPGDTIDDA
                     LGVVPGLTTFGIRSMYTKLGEGITEASQAQDVADHYLELLARIKEAGVDGEISVKPTQ
                     LGLDIDEDMCLGHLRTLSAAAEAHGSYLWIDMEDSSYVDRTIDLYQRLRSTHARTGIC
                     LQAYLRRSAKDVERLLPLGPGVRLVKGAYDEPAAVAFRSRKEVDANYLALAVTLVRES
                     RARDIRLGLGTHDVELIEQIATHAGAAGIPKDGFEVVMLYGIRAKEQQRLARAGYRVQ
                     VLIAYGAAWYPWYMRRLAERPANVLFALRQLLPG"
     gene            complement(7449..7778)
                     /locus_tag="AC162A_02030"
     CDS             complement(7449..7778)
                     /locus_tag="AC162A_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HIR5009644.1"
                     /translation="MKLVVAIVHNEDAGVLVDALLEREYRATRLHSSGGFLKQSNATV
                     IVGVEDAQVDEVITIVRENCNARTQVVNPMPPIMEPGEFFMPYPLEVEVGGATVFVLP
                     VERFERL"
     gene            7878..8900
                     /locus_tag="AC162A_02035"
     CDS             7878..8900
                     /locus_tag="AC162A_02035"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR5009645.1"
                     /translation="MTEPAGGANGPLTVLSAGAAALARSSDLDGAISVIVEAGAAATG
                     AAIAALFSTDPDRARLELLVTLGMSDEQGAGFESMVAEDPSHPIHRAALDRTGTLARQ
                     WIAADGSSMTFADLPMVVAGGGVEACVGVLSFGWEGDHEVGTSEEVLLVAVADLAAAA
                     IASFRTSALATERAEWFERIAHTDPLTGLSNARTLSRVLELEVARAQRQGSEVSVAMF
                     DVDGFTSLNEASGSRAGDQVLRQVAAVLAETVRLVDTVARTGGDEFVVIAPGSAGVTV
                     ARRVLDGIAGLEAIEGHPVSVSAGIARFPQDGADGASLLEAARAALERSGGGASISEA
                     VGEPSA"
     gene            complement(8907..9707)
                     /locus_tag="AC162A_02040"
     CDS             complement(8907..9707)
                     /locus_tag="AC162A_02040"
                     /inference="COORDINATES: protein motif:HMM:NF052375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR5009646.1"
                     /translation="MTDHPNLTCDEVRDLAPLFVTGALDAGEMHAVRDHLAGCADAHA
                     ELAELGEAATALLETTEPAEPPATLKPRLMAAAAADLAEGRHPASAAAANIVSLDKAR
                     ARRGSRLGWLLAAAAVIAAVALGGWNIALRGQLDTAQAYRTGVDQALALAAQPGSVTA
                     FLTAEDGSVSGFSVVGQDGTTKLAVRGLAPTTGAQVYTAWSIEGDNAPVAIGDFTVGS
                     DGVAVATAQAPDATPGAVIALTLEPNAGNPAPMGPVVASGVTRDPAAG"
     gene            complement(9704..10327)
                     /locus_tag="AC162A_02045"
     CDS             complement(9704..10327)
                     /locus_tag="AC162A_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5009647.1"
                     /translation="MSDARLEPATVPELPTGPTDEQLLAGLAAGRLEALDALYERYKT
                     MAYGIARRITADDALAEDVVQEAFLGAWRGADRYVPGRGSVKTWLLSIVHHRAIDAVR
                     RRRPTTELPAEEDGKATPAPLTMPDVWGEVSAGIDREQVNAAVSTLPDAQREALELAY
                     WAGLSQTEIADRLGVPLGTVKSRVRLALVALRRELGAGPLEHAEGPP"
     gene            complement(10324..>11052)
                     /gene="tmk"
                     /locus_tag="AC162A_02050"
     CDS             complement(10324..>11052)
                     /gene="tmk"
                     /locus_tag="AC162A_02050"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR5009648.1"
                     /translation="GTIGSIAYTRAVAEDGETGRPGVFITLEGPDGSGKSSLLLPLGA
                     AIRAVGCDVVTTREPGSTPLGEQVRRLVLDTEPRIDRTGRADALLFAASRAQHVEEVI
                     RPGLARGAVVVCDRYADSSLAYQGAGSGVPMDELRAVQRFATGGLIPDLTILLDLPVE
                     AGLGRKAAEMTRFEAYHDLAYHERVRAAFLAFAADEPERYAIVDATLAADAVLAAAVQ
                     AVGRLAGRLPCLGEPIGSESRMPR"
BASE COUNT         1660 a         3930 c         3929 g         1533 t
ORIGIN      
        1 cctcgaggtc ctcgatcgcc tgctcggtgg agtacagcgg caggtcggcc tcgggcacgc
       61 ccgcttcggt gaggcaggcg tcgacgtagg catccgctgc cgcgcccgcc gcggttccct
      121 gggccggatc ctgtggccgc gcgtcgctgt cgtaataggt ggccgtcgcg tccggacact
      181 ggaagggtcg cgagagcccg atcccgcgct ggtcgatgaa cacgatgtcc atgtgctcgg
      241 cgacgctcgc cgggtagtag tcgctgtagc tgtcggcgac cgagatcccg ctcgagccgg
      301 gaccgccggt gatgacgacg taggtgccga cccgcttccc gctggcgcgc tggatcgcga
      361 acgtgacgtc ccaggtcggg ctgcccgcgg tgttgtgatc gctgggcacg gcgagcgtga
      421 cgcactcgaa ccggctctcc ggacacgcct ccttcttgac gaacgaccag ccggcggcgc
      481 ccggcgacgc ggagggctcg ggtgatgggg cgatcgacgg cgatgcgacg ggggacggct
      541 cgctcgacgg tgacggcgcc ggcgacgagc tggcgggtgt gggagacgcc gatggggccg
      601 ggctcggcga cggcgaggcc ggcgaggccg cagccgcggg gggcatcgtc caggtcggcg
      661 gaatggtgga tccgcaggcc gcgacgacga ggccgaagcc ggccgccgtc accgtcgatg
      721 cgatcgcgcg gcgcagaccg gaccgcgcgg tcgtgcgcgg acgagcgagg atccgcgggg
      781 tcagggacat gtgccgggca gcgccgctag cgtccgcgct gctgccagga cttgccctcg
      841 gtcgcgaccg ccggggcgta cacgatctcg acgtcgtgca tgtccgcgat cgtccgcgcg
      901 ccgagcgccg ccatcgactg gcgcagggcg ccgaacaggt tctgggtgcc gtccgtgacg
      961 tgcgaggggc cgttgaggat ctggtcgagc gacccgaccg tcccgacctt gatccgggtg
     1021 ccccgcggca ggacaggcga cggggccgcc atgccccagt tcgtcccgcg ccccggcgcc
     1081 tcggccgcgc gggcgagcgg cgagcccagc atcacgatgt ccgcgcccgc tgcgaaggcc
     1141 ttggcgaggt cgccaccgcg cttcatgccg ccgtcggcga cgaccggcac gtaggtgccc
     1201 gtctcggcgt ggtactggtc gcgcgccgcg gcgacgtcgg cgaccgccgt gacctggggg
     1261 atgccgatgc ccagcacctc gcgcgtcgtg cacgcggcgc ctggcccgac gccgacgaag
     1321 atcgccgccg cgccctgctc catcagcagg taggcggcct gcgcgttggt ggtgttgcca
     1381 acggcgaccg ggatcggcat gaaccgcgtg aagtcctcga gcgcgagcgg ctcgtagccc
     1441 gtcgcgaggt gccgcgccga ggagacctgc gactggacga gaaacaggtc ggcgccatgc
     1501 tcggcgcaga acggcccgaa ccggcgggcg acgcccggcg tcgccgagac cgccgccttc
     1561 gacccggcgg cgtgcagctc gtcgatgcgg cgcgcgatca gctcctcgcg gatcggcggg
     1621 gcgtacgcct ccgcgagcac gtcgtggacg gcgtcgtcgg gggccgtcgc gatccgctcg
     1681 agaacctcgg aggggtcgtc gtagcgcgtc tggacgccct cgaggttgag cacggcgagc
     1741 ccgccgaggc gggccagctc gcccgacagc ttcacatcga cgaccgcgtc catggccgcg
     1801 gcgaggacgg gaacgccgag ctcgatgccg cagaacgtct gggccaggac gacgtcggcc
     1861 gggtcgatcg tgtcggtgcc gggggccagc gagacgtcct cgaacccgta ggtctggcgg
     1921 atcgtgtcga gcttggagcg gaagatgcgc cggttcgcca gtgtgtcggt caaggcgtcg
     1981 tcctctcgcg catcgggggt gccacccggt gccggacagt ctagcgggag cggcccgcgg
     2041 gaggataatc gccgcccgac gcgacgaacg ggcgcgccga gccagtcgag gacagtgcga
     2101 tggccaaggg caaggcgaag gccgacaccg gcgagggggc ggtcgcggac ccgacggcgg
     2161 acagctacgg ccatccttcg gcgcccgcgg ctcagccgct gtcagcgaag gcctacgagc
     2221 gcgagctcgc ccgcctccag ctcgagctgt cgaagctcca ggagtgggtg catgcccggg
     2281 ggctcaaggt ggtcgtgctg ttcgagggcc gcgacgccgc cggcaagggc ggtgcgatca
     2341 agacgctgac cctggcgctg aacccgcgga tcgcgcgggt cgtcgccctc cccgccccga
     2401 ccgagcgcga gcgcacccag tggtatttcc agcgctacgc cgcccacctg ccggcggcgg
     2461 gcgagatggt cctgttcgac cgcagttggt acaaccgcgc cggggtcgag cgggtgatgg
     2521 gcttcgcgac ggcggacgag atcgaggagt tcttccgctc ctgccccgag ttcgagcgga
     2581 tgctcgtccg gtccgggatc gtgctcatca agtactggtt ctcggtgagc gacgccgagc
     2641 aggagcgtcg gttcgaggcc cgcatcaagg acccgacgaa gcgctggaag ctctcgccca
     2701 tggacctgca gtcgcgcgcc cggtgggtcg agtactcgat cgccaaggac gagatgttcc
     2761 gccacacgga catcaagcag gcgccgtggt gggtggtcaa cgccgacgac aagcgccggg
     2821 cgaggctgaa catgatcgac cacctgttgt cgcaggtgcc ctaccaggac ctcaccccgg
     2881 agcagatcgt gctcccgccg cgtgaacccg accggggcta cgttcgtccg cccctcgacg
     2941 agcagtcgtt cgtgcccgag cgctactgac cgagcggtcg ccgggccagg cggcggccgt
     3001 cgccgtcgag ttcgaaccag cgcgccagcc ccggctcgag cagcaggtcg cggatccgcc
     3061 gcaccgactc ggcgtaggac ggccagatcg cgagcgccgg cgcctcggcc gccggcaccc
     3121 agcgcagccc atcgtgctcg cgcgacagcc gggcaatggc gtcgggccgg atccgggcgg
     3181 cgaagatggc gctcacgacc atgccgtcga cgccctcgtc gaagaacggc gccacctggt
     3241 cgaggtcgta ccaggcctcg acgtccgcgc cccggagggc ggtctcctcc tccacctcgc
     3301 ggatcgccgc catcgggacg cgctcgccgg gctcgacgcc cccggtcacg cactgccaca
     3361 gacccgggaa gatccgcgag gcagaccgcc ggatcagcag gatctcgagg cgatcggcgt
     3421 cgagccgggt gtcggcggcg ccggcggccg gggcgacccg gaacagccag cactcgatca
     3481 ggtcggggcg gaggctcatg ccgctaggcc ccgcatcgag gcggtgacag ccagcaccac
     3541 caagcggtac cgaggtccta tcgcgagccg cgacgcggcg acggccgcgt cccaggcgcc
     3601 cggccgttgc cgaccgcccg atcgtgcacc gttgagcacg aattggccgg tgtcagtgcg
     3661 ccacgatatc gcgatcgagg tcggatcagg ccggtttggt tcggtcattg gcagtggcgc
     3721 gatcacacct gaatggggac tatggctccc gtacgagtca cggtacattt agcgggtttc
     3781 aaccggtact tatggcgcac ctcccctgat gcactcatcc cttccttccc cggttcggcg
     3841 tgccgtgtcg gccggcgccg cgatcctcct cgccgcgaca tcgatcatcg cggctcccgt
     3901 cgcgcgcatc gtgccgccgg ccacggcaca ggccgcgggc agcttcgccg attcccactt
     3961 cacggaagac caggtcttca ccggcctcac gtggcccacc acggtccgat ttgcgtcgga
     4021 cggaacggcc ttcgtcgccg agaagcgggg ggtcatctgg gagtacgaca ccctcagcac
     4081 gccgaacgcc aaccgagtca aggtgcttga catccagacc gaggtctacg acttctggga
     4141 tcgcggcctg ttgagcatcg ccctcgaccc ggacttcctc aacgggcgca actacatcta
     4201 cgtctattac gactacaacg ccccgcccgg ccagacggct ccctattggg cggagccctg
     4261 tccgaacccc ccgggcgcca ccaccgacgg ctgcgtcgtg accagcaagg tcgaccggta
     4321 cacggtcaac ctgaacacct acgcggccag caatcgcgtc aacctgatcg gtgatggccc
     4381 tggcggaacc ggcagcgagt ggtgccagca gtttccgagc cacgcgggcg gggcgctcgc
     4441 cttcgacagc gacgggatgc tgctggtcag cggcggcgac ggtgccagct tcggggggat
     4501 ggactggggc cagctcggag gctcctcggg ctccccgacc ccgaagaacc cgtgcggcga
     4561 cccggtgcct gggttcgtgg tggcgacggc ccagggtggg atgctccgct cgcaggacgt
     4621 tcgcacgcag gcggacccga ccgggctcga cgggacgatc gcccggatcg accctgacac
     4681 cggcaacgcc ccgaacgaca acccgctcaa ggacttcggg ggcgccgatc agaacaccaa
     4741 gcgcatcgtc gccaccgggt ttcgcaatcc gttccggatg accttccggc cagggcacga
     4801 tgacctgtat gtcggcgacg tcggcaacga gacctgggaa gaggtcgatc gctggacgat
     4861 gtcgggcagc acgccgacca acatcccgaa ctatggctgg ccctgctacg agggcaacaa
     4921 ccatcaggcc gcgttcgaga acctcggcac caacatgtgc gacgacctgt acgacgatgg
     4981 gcagaatgcg cccctctaca cgtacacgca cgagggcagc ctgaccccca agggtccctg
     5041 cttccccggg gccgccgacc agacgtcgtc cattacgggc ctcgcgttct acgaaggggc
     5101 caccggcagc tcagtggact acccgaacaa gtacaccggt gccctgttct tcgtcgacta
     5161 ctcgcgcgac tgcctcgggg cgatcctgcc ccagggcaac ggcatccccg acagctccaa
     5221 ggtcgagcag atcgccagcg ggatcgccaa ccccgtggac ctcgtgaccg ggcccggcgg
     5281 cgacctctac tacgtcgacc acaacggcgg acgggtggtc cgcgtgaagt acgtcagctc
     5341 gccgattgcg cgggcgaccg ccgagcccga gggcggcgtg gcaccgatca ccatccacct
     5401 cgatgggtcg acgtcgagtg agcccgatcc cgacttcgcc atcgacgact gggactggga
     5461 cttcttcaac gacggcagct acgacgcctc cggcgaggag gtcgactggc agatcgacac
     5521 ggaaggcgag taccaggtca agctgcgcgt ccacagcacg agcggcctca ccgacacggt
     5581 cacgctgacc gtcgacacgt ccaacgcggc gcccgtcccc gtgatcaacg cgcccgcgga
     5641 ctgtgacgct ccaggctgct ggtcggtcgg cacggacatc agcgtcagcg gcgttgccag
     5701 cgacgccgag gacggcaacc tcagcgcgag ccacttcgat tggtcggtga tcctccacca
     5761 ctgcccggct ggctgccaca cgcacacgat cctgaccaag tcgaacacga aaagcttcag
     5821 cttcgatgcg cccgaccacg agtacccctc gtacctcgag atccgcctca cggcgaccga
     5881 cgacgacgac atctcgagct ccacctccgt cgagctccaa ccgaggacgt cgaccgtcca
     5941 gctcgcctcg tcgcccagcg gcgtgccgct ctcggccggc aacgagaccg agccggcgcc
     6001 ctggacggcc acgttcatcc ggaagggctc ggcgaccatc agcggcccgt tgacccggac
     6061 gatcaacggc aagcgctacc ggttcagcac ctggaacgac tcgcacaccc gcgtgcgcaa
     6121 catcaccgtg agctcgagca agaacctgac ggcgacctac gtccccgacg cgcccgacag
     6181 ctgcgcgggc gcgaccacga cctcgcccaa cgacacgtgg ctgcacgatc gccagagcgg
     6241 caactcggac aaggactggt tccgcttccg cctggcgagc aagcgcgacg tcgtgatcaa
     6301 gctcaaggac ctgcccgtca acgggacgct gcggctgtac aagagctgcg gcaaccaggt
     6361 cgaggagtcc agcaagccgg gcaccaggtc cgagaagatc agccgcacga tcgcaaaggg
     6421 cacctggtac gtgcgcgtgt cctcgccggg caacggctgg agctcgaagc agtacgcgct
     6481 ccggttcaag acggactgac gtcccgcgtc agccggggag cagctggcgc agcgcgaaca
     6541 ggacgttggc cggccgttcg gcgagccggc gcatgtacca cgggtaccag gccgcgccgt
     6601 atgcgatgag cacctgcacc cggtagcccg cgcgggccag ccgctgctgc tccttggctc
     6661 ggatgccgta cagcatcacg acctcgaacc cgtccttggg gatgcccgcc gcgcccgcgt
     6721 gggtcgcgat ctgctcgatc agctcgacgt cgtgggtgcc gaggccgagc cggatgtccc
     6781 tcgcccggct ctcccgcacg agcgtgacgg ccagcgcgag gtagttggcg tcgacctcct
     6841 tgcgcgaccg gaaggccacc gccgccggct cgtcataggc gcccttcacc aggcggacgc
     6901 ccgggccgag cggcagcagt cgctcgacgt ccttcgcgct gcgccgcaaa tatgcttgga
     6961 ggcagatccc cgtccgcgcg tgcgtcgagc gaaggcgctg atagaggtcg atggtccgat
     7021 cgacgtagct gctgtcttcc atgtcgatcc acaggtacga cccatgcgcc tccgcggccg
     7081 ccgacagcgt ccgcagatgg ccgaggcaca tgtcctcgtc gatgtcgagg cccagctggg
     7141 tgggcttcac gctgatctcg ccgtcgacgc ccgcctcctt gatccgggct agcagctcga
     7201 ggtagtggtc ggcgacatcc tgggcctggg acgcctcggt gatgccctcg cccagcttgg
     7261 tgtacatgct ccggattccg aaggtcgtaa ggccgggcac cacgcccagc gcgtcgtcga
     7321 tggtgtcgcc gggcatgaac ttgcgcacgg ccttctgcat gaacggcagc tgcgggagcc
     7381 gctcgcgcag ccacgtgttg cgcgcgaacc acaggaagac cttgcgcatc agctggttca
     7441 tcggggcctc acaggcgttc gaagcgctcg acggggagga cgaacacggt ggcaccgccc
     7501 acctcgacct cgagcggata gggcatgaag aactcgcccg gttccatgat cggcggcatc
     7561 gggttcacca cctgggtccg cgcgttgcag ttctcgcgga cgatcgtgat cacctcgtcg
     7621 acctgggcgt cctcgacgcc gacgatcacg gtcgcgttgg actgcttgag gaagccgccc
     7681 gacgaatgga gccgggtcgc acggtactcg cgctcgagca gggcgtcgac gagcacgccg
     7741 gcgtcctcgt tgtgcacgat ggccacgacc agtttcatcg cggcgaggat agccgaccgc
     7801 gcctcggccg acggggccgc gggcgccgac gaggggtgta cgatgccggg ccgaatgcta
     7861 ttgacgcgga ggctgtcgtg accgaacccg ccgggggcgc caacgggccg ctcaccgtcc
     7921 tctcagcggg cgcggcggcg ctcgcacggt cgagcgacct ggacggtgcg atctccgtca
     7981 tcgtcgaggc cggcgcggcg gccacgggcg cggcgatcgc cgccctgttc agcacggacc
     8041 ccgatcgggc gcgcctggag ctgctggtca cgctcgggat gagcgacgag cagggcgcgg
     8101 ggttcgagtc catggtcgcc gaggacccgt cgcacccgat ccaccgcgcg gcgctggacc
     8161 ggaccgggac gctcgctcgc cagtggattg ccgcggacgg gtcgtcgatg acgttcgcgg
     8221 acctgccgat ggtcgtcgca ggtggcggcg tcgaggcctg cgtgggcgtg ctgtcgttcg
     8281 gctgggaggg cgaccacgag gtcggcacgt ccgaggaggt gctgctggtc gcggtcgccg
     8341 acctcgccgc ggcggcgatc gcgtcgttcc gtacctcggc gctggcgacc gagcgggccg
     8401 agtggttcga gcgcatcgcc cataccgacc cgctgaccgg gctgtccaac gctcgcacgc
     8461 tgagccgcgt cctcgagctc gaggtcgccc gcgcccagcg gcagggcagc gaggtctcgg
     8521 tcgcgatgtt cgacgtcgac ggcttcacgt cgctcaacga ggcctccggg tcccgggccg
     8581 gcgaccaggt cctccgccag gtggccgcgg tgctcgccga gaccgtcagg ctcgtcgaca
     8641 cggtcgcgcg caccggcggc gacgagttcg tcgtgattgc gccgggatcc gccggcgtca
     8701 ccgtcgcccg ccgggtgctc gacgggatcg ccgggctcga ggcgatcgaa ggtcacccag
     8761 tgagcgtcag tgccggcatc gcgcgctttc cccaggacgg ggcggacggg gcgtcgctgc
     8821 tcgaggccgc ccgggcggca ctcgagcgtt cgggcggtgg ggcgtcgatc tcggaggcgg
     8881 tcggcgaacc gagcgcctga gcggggctag cccgcggccg ggtcgcgggt cacgccgctg
     8941 gccacgacag gccccatcgg cgccggattg cccgcgttcg gctccagggt cagcgcgatg
     9001 acggcgcccg gcgtggcatc gggcgcctgg gccgtcgcga cggcgacccc gtccgatccg
     9061 accgtgaagt cgccgatcgc caccggtgcg ttgtcgccct cgatcgacca cgcggtatag
     9121 acctgcgccc cggtcgtcgg cgcgagcccg cgcacggcca gcttcgtcgt cccgtcctgg
     9181 ccgaccacgg agaagcccga gacggagccg tcctccgcgg tgaggaacgc ggtcacgctc
     9241 cctggctggg cggcgagggc gagggcctgg tcgacgccgg tgcggtaggc ctgggcggta
     9301 tccagctgcc cgcgcagggc gatgttccag ccgccgagcg cgacggcggc gatcacggcc
     9361 gcggccgcga gcaaccagcc gaggcgcgat ccccggcgcg cccgagcctt atcgaggctc
     9421 acgatgttcg cggcggctgc cgacgccggg tgccggccct ccgccaggtc cgccgcggcg
     9481 gccgccatca gccgcggctt gagggtcgcc ggcggctcgg cgggttccgt tgtctccagc
     9541 agggcggtgg cggcctcgcc caactccgcc agctccgcgt gcgcatccgc gcagcccgcg
     9601 aggtggtcac gaaccgcgtg catctcgccc gcatccagcg cgccggtgac gaacaggggc
     9661 gctagatcgc ggacctcgtc gcaggtcagg ttcggatggt ccgtcatggc ggaccctccg
     9721 cgtgctcgag gggtccggcg ccaagctcgc gccggagcgc cacgagggcg agccggacgc
     9781 ggctcttcac cgtgcccagc ggcacgccga ggcgatcggc gatctccgtc tgggacaggc
     9841 ccgcccaata ggcgagctcg agggcctcgc gctgggcgtc gggcagcgtg gagacggcgg
     9901 cgttgacctg ctcgcggtcg atccccgcgg atacctcgcc ccacacgtcg ggcatcgtca
     9961 gcggcgcggg cgtggccttg ccgtcctctt cggccggcag ctcggtcgtc ggacgacggc
    10021 ggcgcacggc gtcgatcgcc cggtgatgga cgatggagag gagccaggtc ttcacgctgc
    10081 cgcggcccgg gacgtaccgg tccgcgccgc gccacgcgcc cagaaacgcc tcctggacga
    10141 cgtcctcggc gagggcgtcg tcggcggtga tgcggcgtgc gattccgtag gccatcgtct
    10201 tgtatcgctc atagagcgca tcgagcgcct cgaggcgacc ggcggccaac ccggccagga
    10261 gctgctcgtc ggtcggaccc gtcgggagct ccggcacggt cgccggttcg aggcgagcgt
    10321 cgctcatcgt ggcatgcgtg actcggagcc gatcggttca ccaaggcacg gcagccgacc
    10381 cgccagccgc ccgacggcct gcacggctgc agccagcacg gcatcggcgg cgagcgtcgc
    10441 gtccacgatc gcgtagcgct cgggctcgtc cgccgcgaac gcgaggaacg ccgcccgcac
    10501 gcgctcgtgg taggcgaggt cgtggtaggc ctcgaagcgc gtcatttcgg ccgccttccg
    10561 gcccaggccg gcctcgacgg ggaggtccag gagaatcgtc aggtcgggga tcagaccgcc
    10621 ggtcgcgaac cgctggactg cccgcagttc gtccatcggc acccccgagc cggcgccctg
    10681 gtaggccagc gacgagtccg cataccggtc gcagacgacg acggcgccgc gcgcgagtcc
    10741 ggggcggatg acctcctcga cgtgctgggc gcgcgaggcg gcgaacagga gcgcatcagc
    10801 gcgaccggtg cggtcgatgc ggggctccgt gtcgaggacc aggcgccgga cctgctcgcc
    10861 gagtggggtc gagccgggct cgcgcgtcgt gacgacgtca cagccaaccg cccggatcgc
    10921 ggctccaagg ggcagcaaca ggctcgactt gccggaccca tcgggaccct cgagcgtgat
    10981 gaacacgccc ggccgtcctg tttcgccatc ctccgccacg gcccgagtgt aggcgattga
    11041 tccgatcgtc cc
//
LOCUS       DBNHXO010000098         4321 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3396911, whole genome shotgun sequence.
ACCESSION   DBNHXO010000098 DBNHXO010000000
VERSION     DBNHXO010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3396911"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1079
                     /locus_tag="AC162A_02055"
     CDS             225..1079
                     /locus_tag="AC162A_02055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5009649.1"
                     /translation="MDDSGSTPNVRPATAAEATDVRGMHAATRAAWDEAAERYEGWFD
                     EAVELIRGGGSNLLPVEHDLIGDLRGRCRRAIHLQCAGGRDTLSLWTSGADEVVGVDF
                     SRRMLELAARLSDATGAPARWVEADVLDTPHDLDGTCDLVYTGRGAILWLQDLDAWAA
                     VLRRLLAPEGRLVIFDGHPAEWLFDADPDGRWMLTDYDYFAGPEASRGWAPEYIDRLS
                     IPEGEQHEKFARSWTLGEIVTALLGAGLRVERVAEHPVDWWAGHRDVRPEERGRLPLS
                     FSVVATRG"
     gene            complement(1145..2548)
                     /locus_tag="AC162A_02060"
     CDS             complement(1145..2548)
                     /locus_tag="AC162A_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5009650.1"
                     /translation="MADRILIKNGIVLTQDSSLGELPQADVLIEGSTIAAVGPNLSAE
                     GARVIDAGGDIVIPGFIDTHRHTWETSIRTCAPDFALITYFGSILDKFAPHYRPDDVL
                     AANRWGALECINAGITTLVDWSHIMNTPDHADAAIQGLADTGIRSVFAYGFGNTSLVD
                     WWFGPDYTGSVLTSDGADARRLRKQYFNSDDSRITMALATRGTGFCKPDVVRHDWELA
                     KELGVNITVHVAMDRFGYTKLQILALRDMDLLYPNTTYVHASHFTDEEWALVRDSGGN
                     VSFAPQIEVQMGHGWAPAVTALAYNVPIGLSSDVATTAPSDQFTQMRSILGSERGRKH
                     QEAWDANLDGLEASPGLITSRQVLEWATLGGAQVAGIADRTGSITPGKKADIVIIDGS
                     AVNVAPIIDPVGAVVCAADVSNVKTVIVDGEILKQDYKLAASLTEPRKAVEASRDYLL
                     AKFGEPEPGWLPAKATA"
     gene            complement(2598..3545)
                     /locus_tag="AC162A_02065"
     CDS             complement(2598..3545)
                     /locus_tag="AC162A_02065"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5009651.1"
                     /translation="MTDGGAVLTVGLVGTGRMGSAMARALARAGLPLVVNNRNRDRAQ
                     ALADELGARIADSPADVASAADVTLTMLADDAAVAAVYGGERGLLAGARRGSVLVDLS
                     TVTPDALRAFEAAARAAGVGLLDSPVSGSTATAEAGQLTLMVGGEPGDLERARPVLEP
                     LAKAIFHLGPLGTGAAMKLAVNTLIFGLNGAVSEGLVLAELAGIDRATAYEVLAASAA
                     GAPFVGYKRAAFVDPEGTPVAFSLDLAEKDLRLITALADKLGVSMPQATTNLALVRSA
                     STDGRGGQDFSTVAQELRARARRPVTAAAGRANEEGRES"
     gene            complement(3542..>4321)
                     /locus_tag="AC162A_02070"
     CDS             complement(3542..>4321)
                     /locus_tag="AC162A_02070"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR5009652.1"
                     /translation="AAMTGLRPIAELMFSDFFAVTWDMVINQIAKARYMTDGQVSIPI
                     VLQTANGSGLRFGAQHSQSVENWAMAIPGLKVVAPATPADVVGLMASAVRSDDPVIFC
                     GSKSLFATKGDVPDGDHVVPLGQAVVLREGSDCTIVALAAMVPRAVEAAGRLSADHGI
                     DAEVIDLRSLVPLDVATILTSVAKTSRLFTVEENPRLLGWGAEIASIVADEGFWSLDA
                     PIGRITTPHIPLPSAASLEDIAVPGVDRIVDTVRRRMEEAK"
BASE COUNT          597 a         1525 c         1574 g          625 t
ORIGIN      
        1 tgcgagcgaa gacgcatcgt gcggcgcctc gaacgtggcg accattcccc gcgcggggaa
       61 catctcgagc gacacccgcg ccggttgagc ccggcggtcc gagccaagct cgggtcgcca
      121 cccacgacgg ggccggtact gcgcccggcc ccaggtcgca ctcgttcgcc gtcgtccgtc
      181 tgggcggcgg ctttcctgtt ccggcccgcg tagactcgcg gcccatggac gactcaggct
      241 cgacgcccaa cgtccggccc gccacggcgg ccgaggccac cgacgtgcgt ggcatgcacg
      301 ccgcgacgcg cgccgcctgg gacgaggccg ccgaacgata cgagggctgg ttcgacgagg
      361 cggtcgagct gatccgcggc ggcggctcga acctgctgcc ggtcgagcac gacctgatcg
      421 gcgacctgcg cggccggtgc cgccgggcga tccacctcca gtgcgccggc gggcgcgaca
      481 cgctgtcgct gtggacgtcc ggcgcggacg aggttgtcgg ggtcgatttc agccggcgga
      541 tgctcgagct cgcggcgcgg ctctcggacg cgacgggtgc gccggcccgc tgggtggagg
      601 ccgatgtcct cgacacgccg cacgacctcg acggcacctg cgacctcgtc tacacggggc
      661 gcggcgcgat cctgtggctc caggacctcg acgcgtgggc ggccgtcctt cgccgcctgc
      721 tggcgcccga gggccgcctc gtgatcttcg acggccaccc ggccgagtgg ctgttcgacg
      781 ccgatcccga cggacgctgg atgctcaccg actacgacta cttcgcgggg ccggaggcgt
      841 cccggggctg ggcacccgag tacatcgacc ggctgtcgat ccccgagggc gagcagcacg
      901 agaagttcgc ccggtcatgg accctgggcg agatcgtgac cgcgctgctg ggcgcgggcc
      961 tccgcgtcga gcgcgtcgcc gagcacccgg tcgactggtg ggcagggcac cgcgacgtcc
     1021 gccccgagga gcgtggccgc ctgccgctgt cgttctcggt agtcgcgacg cggggctgag
     1081 aatcgaggaa cccggacggc ggtgccggcc gggctcctca gtgtcggcgg ggtagacggc
     1141 tgggtcaggc ggtcgccttg gccggcagcc agcccggctc cggctcgccg aacttggcca
     1201 gcaggtagtc gcgggacgcc tcgacggcct tgcgcggctc cgtgagcgac gcggccagct
     1261 tgtagtcctg cttgaggatc tcgccgtcga cgatgacggt cttcacgttc gagacgtcgg
     1321 cggcgcagac gacggctccg accgggtcga tgatcggggc gacgttcacg gccgagccgt
     1381 cgatgatcac gatgtcggcc ttcttgccgg gcgtgatcga gccggtcctg tccgcgatcc
     1441 cggcgacctg ggccccgccc agcgtggccc actcgaggac ctggcgcgag gtgatcagcc
     1501 cgggcgacgc ctcgagcccg tcgaggttgg cgtcccatgc ctcctggtgc ttgcggcccc
     1561 gctcggagcc caggatcgag cgcatctggg tgaactggtc cgatggggcc gtcgtggcga
     1621 cgtccgacga gagcccgatc gggacgttgt atgcgagcgc cgtgaccgcc ggggcccagc
     1681 cgtggcccat ctggacctcg atctgcggcg cgaacgacac gttgccgccc gagtcgcgga
     1741 ccagcgccca ctcctcgtcg gtgaagtgcg aggcgtggac atacgtggtg ttcgggtaga
     1801 ggaggtccat gtcccggagg gccaggatct ggagcttcgt gtagccgaac cggtccatgg
     1861 cgacgtggac ggtgatgttg acgcccagct ccttcgcgag ctcccagtcg tggcgcacga
     1921 cgtccggctt gcagaacccg gtgccgcgcg tggccagggc catcgtgatc cgcgagtcgt
     1981 cggagttgaa gtactgcttc cgcaggcggc gggcgtcggc cccgtccgag gtgagcacgc
     2041 ttcccgtgta gtccggcccg aaccaccagt caacgaggga ggtgttgccg aagccgtacg
     2101 cgaacaccga ccggatgccc gtgtcagcga gcccctggat cgccgcgtcg gcgtggtcgg
     2161 gggtgttcat gatgtgcgac cagtccacga gggtcgtaat gcccgcgttg atgcactcga
     2221 gcgcacccca gcggttcgcg gcaaggacgt cgtccggccg gtagtggggg gcgaacttgt
     2281 cgaggatcga cccgaaatag gtgatcagcg cgaagtccgg ggcgcaggtc cggatcgatg
     2341 tctcccacgt gtggcggtgg gtgtcgatga agcccgggat cacgatgtca ccgccggcgt
     2401 cgatcaccct cgcgccttcg gcggagagat tgggcccgac ggccgcgatc gtgctgccct
     2461 cgatgagcac gtcggcctgc ggcagctcgc cgagggacga gtcctgggtg agaacgatcc
     2521 cgttcttgat aaggatccgg tcagccatgg gtgcctcctg catgcgacgt catcggtgac
     2581 gagtgctcgg acgatctcta gctctcccgc ccctcctcgt tggcgcgccc cgcggcggcc
     2641 gtgaccggcc gtcgggctct cgcgcgcagc tcctgtgcga cggtcgagaa gtcctggccc
     2701 ccgcggccgt ccgtggatgc ggaccgcacg agagcgaggt tggtggtcgc ctggggcatc
     2761 gacacgccga gcttgtcggc caacgcggtg atcaggcgca ggtccttctc ggcgaggtcg
     2821 agcgagaagg ccacgggcgt gccctcgggg tccacgaacg cggcccgctt gtaaccaacg
     2881 aacggcgccc cggccgcgct tgcggcgagc acctcgtacg cggtcgcacg gtcgatcccc
     2941 gcgagctcgg ccaggacgag gccctcggag acggccccgt tcagcccgaa gatcagcgtg
     3001 ttgaccgcca gcttcatcgc cgcaccggtg cccagcgggc cgagatggaa gatcgccttc
     3061 gccagcggct ccagcacggg gcgggcgcgc tcgagatcac cgggctcgcc gccgaccatc
     3121 agcgtcagct gtcccgcctc cgccgtcgcc gtcgagccgc tcaccggcga gtcgagcagg
     3181 ccgacgcctg cggcgcgggc ggccgcctcg aacgcgcgca acgcgtcggg cgtgaccgtg
     3241 ctcagatcga cgagaaccga gccgcgccgg gcgccagcca gcagcccgcg ctcgcccccg
     3301 tagaccgcgg ccaccgccgc gtcgtcggcg agcatcgtga gcgtgacgtc cgccgccgag
     3361 gcgacgtcgg ccgggctgtc cgcgatgcgc gcgcccaact cgtccgcgag ggcctgcgca
     3421 cggtcgcggt tccggttgtt gacgacgagg ggcaaaccag cgcgggcgag cgcgcgggcc
     3481 atcgcggagc ccatccgccc ggtgccgacc aggccgaccg taaggaccgc accgccgtcg
     3541 gtcacttcgc ctcctccatc cggcggcgga cggtgtcgac gatccggtcc acgccgggga
     3601 cggcgatgtc ctcgagcgac gccgcggagg gcagcgggat gtgcggcgtc gtgatccggc
     3661 cgatcggggc gtcgaggctc cagaacccct catcggcgac gatcgaggcg atctcggcgc
     3721 cccagccgag cagacgcggg ttctcctcga cggtgaacag gcgactggtc ttggcgaccg
     3781 acgtcaggat ggtcgcgacg tcgagcggca cgaggctgcg caggtcgatg acctcggcgt
     3841 cgatcccgtg gtcggcggac agccgccccg cggcctccac ggcccgcggc accatcgcgg
     3901 cgagcgccac gatcgtgcag tccgagccct cgcgcaggac gaccgcctgc cccaggggga
     3961 cgacgtgatc gccgtcgggg acgtcgccct tggtcgcgaa cagcgacttg ctcccgcaga
     4021 agatcaccgg gtcatcgctg cgcacggccg aggccatcag cccgaccacg tccgcgggcg
     4081 tggccggcgc cacgaccttg aggcccggga tcgccatggc ccagttctcg acgctctggc
     4141 tgtgctgcgc cccgaaccgc aggcccgagc cgttggccgt ctgcagcacg atcgggatcg
     4201 agacctggcc gtcggtcatg tagcgggcct tggcgatctg gttgatcacc atgtcccagg
     4261 tgacggcgaa gaagtcgctg aacatcagct cggcgatcgg gcgcaggccg gtcatcgccg
     4321 c
//
LOCUS       DBNHXO010000099         3171 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2999946, whole genome shotgun sequence.
ACCESSION   DBNHXO010000099 DBNHXO010000000
VERSION     DBNHXO010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2999946"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1295
                     /locus_tag="AC162A_02075"
     CDS             102..1295
                     /locus_tag="AC162A_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR5009653.1"
                     /translation="METAPFLLDIGLALLLAAGFGWVARKLTLPAVVGYLAAGLVVSQ
                     FTPGYVADRHQIELLAEVGVVLLLFEVGIEVDLGRLRREHGGLLWASPAQIGISTAIA
                     AVVLYALGVAPLGAALVGVSVAMSSSVVIVNITRSRRRTTDKPTEEGLLGWSVLQDVT
                     GVVLSTVILAVSGYAGQPLPMALALLAAFGLLAVAADQVLPRILRRLRSEHDLFLIVS
                     VASGLALAAAGAVFFGVPAALAAFVAGLAISDRPETAEARRRLLPFRDVFAVLFFVAV
                     GSLIDPGSLPGAAPWIALLIGLLVVAKVLVAWVLVRVARLEARPLQLAVGLGQMGEFG
                     YVLAGIGLTANVVSSELFTAVLASIVITIAGSAILVRFAGKAPSPADAGPPAASLRLG
                     RPPSR"
     gene            complement(1285..2070)
                     /locus_tag="AC162A_02080"
     CDS             complement(1285..2070)
                     /locus_tag="AC162A_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009654.1"
                     /translation="MSASSAWDRAGSLPVPVSGRRLPEVAALRAELPDVDALFTFMRD
                     AELRFATLRMRLEEHTWTARGEEVLVHDVTLRHPGEVKVLTSVPVDGTTDGYEAWVSD
                     GITVQTYVASRKLGTQRPVRPMVRGVTGKNDLPGRSRVYAPLTALPMETLPDLFIHPG
                     GYCQNVLATGACRVVGSSTVAGREAIVLECDHPRTIELTADRPDFSIRISVDRSDGVI
                     LRLEESIGGQVTRDAIVTSYVPNASLPPSAFAFTFPPDTTVIY"
     gene            2191..2805
                     /locus_tag="AC162A_02085"
     CDS             2191..2805
                     /locus_tag="AC162A_02085"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR5009655.1"
                     /translation="MEVTAMGRAGIRISRRELLRRSLAAGIGIWVIETVAGTIGFLWP
                     SAASAALRARVGTVDDLVAANPGIPFRDGFPVYVAQALAFVVLVDPSQGFVPGVDESG
                     TGAGVNVIALSKICPHLGCRPNPCLEDWWMHCPCHQSRYDRLGIKAAGEEYGPAPRGM
                     DRFAVEVDRTGVLTIDTTRVVLGPLPVAVGQPGLILPRVPHGCA"
     gene            2817..>3171
                     /locus_tag="AC162A_02090"
     CDS             2817..>3171
                     /locus_tag="AC162A_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009656.1"
                     /translation="MLRLYPGPWRRRYSVEVAALLDEHPPGWRDRLDLLRGALDAHLH
                     PWTAPTWPVIAAAIGGIAWTFAGAVALGQPAPPDWPGYLEETLAIFLGAVPLLALAAL
                     GASTRLGDRDPATARV"
BASE COUNT          381 a         1158 c         1120 g          512 t
ORIGIN      
        1 accgaatcgt agatctcctg ctcgaccctg gccgcgttga ggtagtgggc gaggacgtcg
       61 tcgccgaaga tcccgattcg accggccggt atcctccggc cgtggagacg gcgcccttcc
      121 tgctcgacat cggcctggcc ctcctgctcg cggcaggctt cggctgggtc gcccgcaagc
      181 tcacgctgcc ggccgtggtc ggctacctcg cggccggact cgtggtctcc cagttcacgc
      241 ccggctacgt cgcggaccgc caccagatcg agctgctggc cgaggtcggc gtcgtgctgc
      301 tcctgttcga ggttggcatc gaggtcgacc tcggccgtct ccgtcgcgag cacggcggcc
      361 tgctgtgggc gtcgccggcg cagatcggca tctcgaccgc gatcgccgcc gtcgtgctct
      421 acgcgctcgg cgtggcgccg ctgggtgccg cgctggtcgg cgtgtcggtg gccatgtcga
      481 gctccgtcgt catcgtcaac atcacgcgtt cgaggcgacg gacgacggac aagccgaccg
      541 aggagggcct gctcgggtgg agcgtgctcc aggacgtgac cggcgtggtg ctgtcgaccg
      601 tgatcctcgc ggtgtccggg tatgccgggc agccgctgcc gatggcgctc gccttgctcg
      661 cggccttcgg actcctggct gtcgcagcgg accaggtgct ccctcgcatc cttcgacgat
      721 tgcgatcgga acacgacctg ttcctgatcg tgtccgtcgc atcgggcctc gcgctcgccg
      781 cggcaggcgc cgtcttcttc ggcgtgccgg cggccctggc cgcgttcgtc gccggcctcg
      841 cgatcagcga ccgccccgag acggccgagg cgcggcgccg cctgctgccg ttccgcgatg
      901 tgttcgccgt gctgttcttc gtggcggtcg gctcgctgat cgacccgggc agcctccccg
      961 gcgcggcccc ctggatcgcc ctgctgatcg gcctgctcgt cgtggccaag gtcttggtgg
     1021 cgtgggtgct cgtcagggtg gcgcgactcg aggcccgtcc actccagctc gcggtcggcc
     1081 tgggccagat gggcgagttc ggctacgtcc tcgccgggat cggcctgacc gcgaacgtcg
     1141 tctcgagcga gctgttcacg gccgtcctgg cctcgatcgt gatcaccatc gccggctcgg
     1201 cgatcctggt ccggttcgcc ggcaaggcac cgtcaccggc ggacgcgggc ccacccgccg
     1261 cgtcgctccg cctcggccgg ccgcctagta gatgacggtc gtgtccggcg ggaacgtgaa
     1321 cgcgaacgcc gacggcggca gcgacgcgtt ggggacgtag ctggtcacga tcgcgtcccg
     1381 ggtgacctgg ccgccgatcg actcctcgag ccgcaggatc acgccgtccg agcggtcgac
     1441 cgagatccgg atcgagaagt ctggccggtc cgcggtcagc tcgatcgtcc gcgggtggtc
     1501 gcactcgagc acgatcgcct cacgcccggc caccgtcgac gagccgacca cgcggcaggc
     1561 gcccgtcgcg agcacgttct ggcaataccc gcccggatgg atgaacaggt ccggcagggt
     1621 ctccatcggc agcgcggtca acggggcgta gacacgggat cgccccggca ggtcgttctt
     1681 gcccgtgacg ccgcggacca tcggccgcac gggtcgttgg gtgcccagct tgcgtgacgc
     1741 cacgtaggtc tggacggtga tcccgtccga gacccaggcc tcgtagccgt cggtcgtgcc
     1801 gtcgacgggc accgaggtca ggaccttcac ctcgcccggg tgccggaggg tgacgtcgtg
     1861 gacgagcacc tcctcaccac gcgcggtcca ggtgtgctcc tccaggcgca tccggagcgt
     1921 tgcgaagcgc agctcggcgt cgcgcatgaa cgtgaacagc gcgtcgacgt cgggcagctc
     1981 ggcgcgcagc gcggcgacct cggggagccg gcgaccggac acggggacgg gcagggaccc
     2041 ggcgcggtcc caggcgctgc tcgcggacat cgcggggatt gtagcggggc ccgcgtcgcc
     2101 gaaggccctt gactggcggg acgcagggtg ttatatcgaa cgacgatata acgacaatag
     2161 gactagcgat cccgtcgtat ccgacagccg ttggaggtca ccgccatggg ccgcgcgggc
     2221 atccggatct cgcgtcgcga gctgctccgc cgctcgctcg ccgctgggat cgggatctgg
     2281 gtcatcgaga ccgtcgccgg gacgatcggg ttcctgtggc cgtcggcagc cagcgccgcc
     2341 ctgcgggcgc gagtcggcac cgtggacgac ctggtcgccg cgaaccccgg gatcccgttc
     2401 cgcgacgggt tccccgtgta cgtcgcgcag gcgctggcgt tcgtcgtgct cgtcgatccg
     2461 agccaaggat tcgtgcccgg cgtcgacgag tccggcaccg gggcgggcgt caacgtcatc
     2521 gccctgtcca agatctgccc gcacctcggc tgccgtccca acccctgtct cgaggactgg
     2581 tggatgcact gtccatgcca ccagtcgcgc tacgatcgcc tcgggatcaa ggccgcaggc
     2641 gaggagtacg gccccgcgcc gcgcggcatg gaccgcttcg ccgttgaggt cgaccgcacg
     2701 ggcgtgctga cgatcgacac caccagagtc gtcctggggc ccctgccggt ggccgtcggg
     2761 cagcccgggc tcatcctgcc gcgcgtcccg cacggctgcg cgtgaccgtg gacccgttgc
     2821 tccgcctgta tccgggtccc tggcgacgcc ggtattcggt cgaggtcgcg gccctcctcg
     2881 atgagcaccc tcccggctgg cgcgaccggc tcgacctgct ccgcggcgcg ctcgacgccc
     2941 acctccaccc gtggaccgca ccgacatggc cggtcatcgc cgccgcgatc ggcggcatcg
     3001 cctggacctt cgccggtgcg gtggcgctcg gccagccggc gcccccggac tggcccggct
     3061 atctcgagga gacgctcgcg atcttcctcg gcgcggtgcc gttgctcgcg ctggccgccc
     3121 tcggtgcctc cacccggctc ggcgaccgcg acccggcgac cgcacgggtc g
//
LOCUS       DBNHXO010000100         1592 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_24364, whole genome shotgun sequence.
ACCESSION   DBNHXO010000100 DBNHXO010000000
VERSION     DBNHXO010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_24364"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..896
                     /locus_tag="AC162A_02095"
     CDS             48..896
                     /locus_tag="AC162A_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR5009657.1"
                     /translation="MDDDLLPIGRFARLTGLSIGALRHYDEEGVLAPADVDPATGYRR
                     YRRDQVATARAIAALRDLELSLPAIRALLDADDATDRGSILRDERVRLEARTARLQRA
                     LHRLSVLAATNPNPDPATKEIPVPTPPPPPELAPELNRALGAGLYNRCWDLMEVEDRT
                     LDQDDELIATAHASAWHWRQVGHVANQSRGHWMCSRVYAVLGRPEPALYHARRCVDIV
                     EGGGEGIEDWDAPSAYEAMARALAIGGDLAGAAEWKAKAAVALQAIAEPEDRTVVEGD
                     LATLPV"
     gene            922..>1592
                     /locus_tag="AC162A_02100"
     CDS             922..>1592
                     /locus_tag="AC162A_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR5009658.1"
                     /translation="MHDVKLGVLLWNQATDWPSYLDAVRKVDKLGYSHLWAWDHLYAI
                     FGDPYQPIFEGWASLSAAAVATEKTQLGLLVGANTFRNPGLAAKLASTLDHISGGRAI
                     LGLGGAWMEPEHTAHGIDFGTGFGQRLDWLDEAVGACRDLLDGKSVTSAPGGHYQFND
                     LRHAPTPVQAHLPIMIGGSGEKKTLRTVAKYADLWNGMGPLDFITHKVGVLKQHCEDV
                     GRDPS"
BASE COUNT          242 a          602 c          534 g          214 t
ORIGIN      
        1 aaattcgccc ttgactctcg ggtaggtcga gggttcacga tctgcgcatg gacgacgacc
       61 tgctgcccat cgggcgcttc gcgcggctga ccgggctctc gatcggcgcc ctccggcact
      121 acgacgagga gggcgtactc gcgcccgccg atgtcgaccc ggctaccggc taccggcgct
      181 accggcgcga ccaggtcgcg acggcgaggg cgatcgcggc cctccgcgac ctcgagctgt
      241 cactgccggc gatccgcgcg ctcctcgacg ccgacgatgc cactgaccgt ggctcgatcc
      301 tgcgcgacga gcgcgtccgc ctcgaggcgc gtaccgcgcg gctgcagcgc gccctgcacc
      361 ggctcagcgt cctcgccgcc accaacccga accccgatcc agccacgaag gagatccccg
      421 tgccgacccc tcccccgccc cccgagcttg cccccgagct gaaccgcgcc ctcggtgccg
      481 gcctgtacaa ccgctgctgg gacctgatgg aggtcgagga ccggaccctc gaccaggacg
      541 acgagctgat cgcgaccgcc catgcgtcgg cctggcactg gcggcaggtg gggcacgtcg
      601 ccaaccagtc gcgcggccac tggatgtgtt cccgcgtcta cgccgtcctc ggccgcccgg
      661 agcccgcgct ctaccacgcc cgccggtgcg tcgacatcgt cgagggtggc ggcgagggca
      721 tcgaggactg ggacgcgccg tcggcgtacg aggccatggc ccgcgccctc gcgatcggcg
      781 gcgatctcgc cggggcagcc gaatggaagg ccaaggccgc ggtcgccctg caggcgatcg
      841 ccgagccgga ggaccgcacc gtcgtcgaag gcgacctggc gaccctcccc gtgtgaccat
      901 cgggcgtgcg acgatagccg catgcacgat gtgaagctgg gcgtcctcct ctggaaccag
      961 gccaccgact ggccgtcata cctcgacgcc gtccgcaagg tcgacaagct cgggtactcg
     1021 cacctctggg cgtgggacca cctctacgcg atcttcggcg acccgtacca gccgatcttc
     1081 gagggctggg cgagcctgtc cgcggccgca gtcgcgacgg agaagacgca gctgggcctg
     1141 ctcgtcggcg ccaacacgtt ccgcaacccg ggcctcgcgg ccaagctcgc ctcgacgctc
     1201 gaccacatct cgggtggtcg cgcgatcctc ggcctcggcg gggcctggat ggagcccgag
     1261 cacacggccc acgggatcga cttcgggacc ggcttcgggc agcggctcga ctggctcgac
     1321 gaggccgtgg gcgcctgccg tgacctgctc gacggcaagt ccgtgacctc ggcgccgggc
     1381 ggccactacc agttcaacga tctgcgccac gcacccacac cggtgcaggc ccacctgccg
     1441 atcatgatcg gcggcagcgg cgagaaaaag accctgcgaa cggtcgcgaa gtacgccgac
     1501 ctgtggaacg gcatggggcc gctcgacttc atcacccaca aggtcggggt cctgaagcag
     1561 cactgcgagg acgtcgggcg cgatccgtcc ga
//
LOCUS       DBNHXO010000101         2174 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_817658, whole genome shotgun sequence.
ACCESSION   DBNHXO010000101 DBNHXO010000000
VERSION     DBNHXO010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_817658"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="AC162A_02105"
     CDS             <1..1057
                     /locus_tag="AC162A_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009659.1"
                     /translation="RSNGFAERLDPDRASGGYHRSLVSRGVNSAELRSGATGTSSTAT
                     SSVLQPPVTPSLVSLGLTFGQVNTRNLPIVAARTELVVPVAAKGAKRMPEGTQVGVRW
                     DPILLDARPTAPVATTPSPAPGTSPAPLNAPEVDLVVAEQLGSVVTLAPAERTGRGLN
                     VGVTYPSAPGLYRLVVTLHNSTGVAYDAPTQSKLTSALVRVGGLYSAAFGAPSSLAMA
                     AGSTSTVGVRVVNAGSQGWDVESSTPPSEPDSLLIWLRTQRTAARVVGTWVSAQGMQV
                     PGPVSTALDPKAAEPGGSAAVGLAVVAPAQAGDYLLLLDVVSPTHGTLSTLGSTPALI
                     RVSVTGQAPAASPSPAP"
     gene            1115..>2174
                     /locus_tag="AC162A_02110"
     CDS             1115..>2174
                     /locus_tag="AC162A_02110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5009660.1"
                     /translation="MSTDRRPVGPGRADQASDPLRILLVAPPMLPVPPPTYAGTERVV
                     AALGDELHRRGHHVALVAPGDSRVPYEHIRTIDESLWSSGYAGRLEGFLQHTIEIAWR
                     AADRFDIVHAHMEHDSFVFAEHCATPVITTLHGRLDTEGMPELLAAHPNVPLVAISES
                     QRRFFPDQRWVSTIYHGLPLGDMPFEDQAGDYLLFVGRITPEKGIEDAIALSRATGIR
                     LKVAAKVHLESEHRHFREVVQPAIDDDVIDFLGELGPEERDPLYARALATVMLGAWPE
                     PFGLVAIESMATGTPVIARRAGALVETVIHGESGFIVDDVAEAALAVGRVPELDRRAI
                     RDSALARFSPARMADEYEV"
BASE COUNT          280 a          817 c          785 g          292 t
ORIGIN      
        1 gcgctcgaac gggttcgccg agcgcctcga tcccgaccgg gcgagtggtg gctaccaccg
       61 ctcgctggtg tcgcgcggcg tgaactcggc cgagctgcgg tcgggcgcca ccgggacgtc
      121 gtcgacggcg acgtcctcgg tcctgcagcc gccggtgacc ccgtccctgg tctcgctggg
      181 gctcacgttc ggccaggtca atacccgcaa cctcccgatc gtcgccgcac ggaccgagct
      241 ggtcgtgccc gtcgccgcga agggggcgaa acggatgccc gaaggcacgc aggtcggggt
      301 ccgctgggat ccgatcctgc tcgacgcgcg cccgacggcg cccgtggcca cgaccccgtc
      361 gccagccccg gggacctcgc cggcgcccct caacgcgccc gaggtggacc tcgtcgtcgc
      421 cgagcagctg ggctcggtcg tgacgctcgc gccagcggaa cgaacgggac gcggcctcaa
      481 cgtcggcgtc acctatccga gcgcacccgg gctgtaccgc ctcgtcgtga cgctccacaa
      541 ttcgaccggc gtcgcctacg acgccccgac ccagtcgaag ctcacgtccg cgctcgtccg
      601 cgtcggcggc ctgtactccg cggcgttcgg cgccccgtcg tcgctcgcca tggcggccgg
      661 ctcgacgtcg acggtcggcg tccgggtggt caacgcgggc tcccagggct gggacgtcga
      721 gtccagcacg ccgccgagcg agccggactc gctgctgatc tggctgcgca cccagcgcac
      781 ggccgcacgg gtcgtcggga cgtgggtgtc ggcgcagggc atgcaggttc ccggccccgt
      841 gtccacggcg ctggatccca aggcggccga gccgggcggg tcggcggcgg tcggcctcgc
      901 cgtcgtcgcg ccggcgcagg cgggcgacta cctcctgctg ctcgacgtcg tctcgccgac
      961 ccacggcacc ctctcgacgc ttggcagcac gccggcactg atccgcgtct cggtgaccgg
     1021 gcaggcaccc gccgcctccc cgagccccgc gccctaggac tccgccgggc gccgtcggac
     1081 gcccgatccg ggccgggatg attgaatggg cgccatgagc accgaccgca ggccggttgg
     1141 cccggggcgc gcggaccagg cctccgaccc gctgcgaatc ctcctggtcg cgccgccgat
     1201 gctgccggtg ccgccgccga cgtacgcggg caccgagcgc gtggtcgccg cgctgggcga
     1261 cgagctccac cgccgcggcc accacgtcgc gctcgtggcg cccggcgact ccagggtccc
     1321 ctacgagcac atccggacca tcgacgagag cctgtggtcg tcgggctacg cgggccggct
     1381 cgaggggttc ctgcagcaca cgatcgagat cgcctggcgg gcggcggacc gcttcgacat
     1441 cgtccacgcc cacatggagc acgacagctt cgtgttcgcc gagcactgcg cgacgcccgt
     1501 gatcacgacc ctgcacggcc gcctggacac cgagggcatg cccgagctgc tggcggcgca
     1561 cccgaacgtg cccctggtcg cgatcagcga gagccagcga cggttcttcc ccgaccagcg
     1621 ctgggtgtcg acgatctacc acggcctgcc gctgggcgac atgccgttcg aggatcaagc
     1681 gggcgactac ctgctgttcg tcgggcgcat cacccccgag aaggggatcg aggacgcgat
     1741 cgcgctcagc cgggcaaccg gcatccgcct caaggtggcg gccaaggtcc acctggagtc
     1801 agagcaccgg cacttccgcg aggtggtgca gccggcgatc gacgacgacg tgatcgactt
     1861 cctgggcgag ctcggccccg aggagcgcga tccgctctac gcgcgggcgc tggcgacggt
     1921 gatgctgggt gcctggcctg agccgttcgg gctggtggcg atcgagtcga tggcgaccgg
     1981 gacgccggtg atcgctcggc gggccggggc gctcgtcgag accgtgatcc acggcgagag
     2041 cgggttcatc gtggacgacg tggccgaggc ggcgctcgcg gtcggccgcg tgcccgaact
     2101 cgaccggcgg gcgatccgcg acagcgcgct ggcccggttc tcgcccgcgc gcatggccga
     2161 cgagtacgag gtcg
//
LOCUS       DBNHXO010000102         2179 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3596168, whole genome shotgun sequence.
ACCESSION   DBNHXO010000102 DBNHXO010000000
VERSION     DBNHXO010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3596168"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="AC162A_02115"
     CDS             <1..1244
                     /locus_tag="AC162A_02115"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019068520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR5009661.1"
                     /translation="PDRPVFLPNKDGHDAWVNSRALEIAGITADTPDPHDGRIARNPD
                     GSPLGTLHEGAQYLVWRHLPPTTDAEREQGLLESQAHLHAIGITNWQDAHVDPEDHAT
                     YLRVAGRGDLTARVVGALWWEHDAGLDQVDGLVARRADGPVGRYSATSVKLMLDGIIE
                     NYTASVVDPYFDADGNQTENRGMDFIDPDVLVQAVPMLDALGFQVHFHALGDRAVRIG
                     LDAIEAARKANGPSDTRPHLAHLQVVHPDDLPRFRQLGALANVQPLWAVHEGQMDDLT
                     IPFLGPVASARQYPLRSFLRHGATVVMGSDWSVSTADPFPQIDIAVNRVVPGHEGLQP
                     PFMPEEAIELAEAVIAFTAGSAHASHLDEAGTIEVGKLADLVVLDRDLFDRGAGGIAD
                     TRVVATFVDGVAVHETPALEG"
     gene            1427..1612
                     /locus_tag="AC162A_02120"
     CDS             1427..1612
                     /locus_tag="AC162A_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009662.1"
                     /translation="MHPLAIYMAQVHQNDLMAEADANRLAKQARQPREAGIVASFKSF
                     VRNLVTTNDAHSAASPA"
     gene            complement(1737..>2179)
                     /locus_tag="AC162A_02125"
     CDS             complement(1737..>2179)
                     /locus_tag="AC162A_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR5009663.1"
                     /translation="RVTFTAPPIPVIVDASVAVETYFGSPLATEAWRRWATEQRTLLA
                     PPIVWPEVANALIRRHHVPPGIVARHLDGLVAAGLQTADRGPAGVAFAAGLAAHRGLS
                     VYDATALWLAIDVDGELATFDRALVAAAKAEGVPIAEGLEPIGP"
BASE COUNT          345 a          806 c          728 g          300 t
ORIGIN      
        1 tccccgatcg accggtgttc ctgcccaaca aggacggcca cgacgcatgg gtcaacagcc
       61 gcgccctcga gatcgcgggt atcaccgccg acacccccga tccccacgat ggccggatcg
      121 cgcgcaaccc ggacggttcg ccgctgggga cgctgcacga gggcgcccag tacctggtct
      181 ggcgccacct cccgccgacc acggacgccg agcgcgagca gggcctgctc gagagccagg
      241 cccacctgca cgccatcggg atcacgaact ggcaggacgc ccacgtggac cccgaggacc
      301 acgccacgta cctccgggtg gccggccgcg gcgacctgac ggcgagggtc gtcggggcgc
      361 tgtggtggga gcacgacgcg gggctcgatc aggtcgatgg gctggtcgct cgcagggctg
      421 acggcccggt ggggcgctat tccgccacga gcgtgaagct gatgctcgac ggcatcatcg
      481 agaactacac ggcgtccgtc gttgatccgt acttcgacgc cgacggcaac cagaccgaga
      541 accggggcat ggacttcatc gatcccgacg tcctcgtcca ggccgtgccc atgctcgacg
      601 cgctcgggtt ccaggtgcac ttccacgcgc tgggggaccg ggcggtgcgc atcggcctgg
      661 acgccatcga ggcggcacgc aaggccaacg gcccgagcga cacgcggccg cacctcgccc
      721 atctccaggt ggtccacccc gatgacctgc cgcgcttccg gcagctgggc gcgctcgcca
      781 acgtccagcc cctgtgggcg gttcacgagg gccagatgga cgatctcacg atcccgttcc
      841 tcggtccggt cgcgtcggcc cggcagtacc cgcttcgctc gttcctgcgc cacggggcga
      901 cggtcgtgat gggctcggac tggtccgtgt cgacggccga cccgttcccg cagatcgaca
      961 tcgccgtcaa ccgcgtggtc ccgggccacg agggcctgca gccgccgttc atgccggagg
     1021 aggccatcga gctcgccgaa gcggtgatcg cgttcaccgc cggctccgcc cacgccagcc
     1081 atctcgacga ggcgggcacg atcgaggtcg gcaagctcgc cgacctggtc gtcctcgacc
     1141 gggacctgtt cgaccgcggc gccggcggca tcgccgacac gcgtgtcgtg gcgacgttcg
     1201 tcgatggggt cgccgtgcac gagacgcccg ccctcgaggg ctgagcgcag ggtcacgtcg
     1261 cggggtcgtc ccccgcccac tccgtcctcg gtcggatgac gtagtcgatt cgaggttccc
     1321 ggtccatcgg ccgtggcgtg ccggcgcgcg atagtgcctt cagcggcaac gaacaaccag
     1381 acagcgcacc accgaagctg ccgcaggaaa ggaggtcatc gagatgatgc acccgctagc
     1441 gatctacatg gcccaggttc accagaacga cctgatggca gaggccgacg caaaccgcct
     1501 cgcgaagcag gcacgccagc cccgagaggc aggcatcgtg gcctccttca agagcttcgt
     1561 ccgcaacctg gtcaccacga acgacgcgca cagcgccgct tcgcccgcct gatcgcacac
     1621 aacggccggc gggcctccct cccagccggc tcgcatcgcc cggacccctc ctccggtccg
     1681 ggagcatcct ccacgcagac gcccgcccgc cctcccaggg gcgggcgttt ctgcgtctag
     1741 ggaccgatcg gctcgagacc ctccgcgatc ggcacccctt cggccttcgc cgccgccacg
     1801 agggctcgat cgaacgtggc cagctcgccg tccacatcga tggcgagcca cagtgccgtc
     1861 gcgtcgtaga ccgacagccc gcgatgcgcc gcaaggcctg ccgcaaatgc gacgccggcg
     1921 ggtccgcgat cggcggtttg gaggcccgcc gcgaccaggc catcgaggtg tcgtgcgacg
     1981 atgcccggcg gtacgtgatg gcgccggatc agcgcgttgg caacctcagg ccagacaatc
     2041 ggcggcgcga gaagcgtcct ttgctcggtc gcccagcgtc tccaggcctc ggtggcgagc
     2101 ggactaccga agtaggtctc gacggcgacc gaggcgtcga cgatgaccgg tatgggcgga
     2161 gcggtaaacg tcacacgct
//
LOCUS       DBNHXO010000103         1820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2406513, whole genome shotgun sequence.
ACCESSION   DBNHXO010000103 DBNHXO010000000
VERSION     DBNHXO010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2406513"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="AC162A_02130"
     CDS             complement(<1..1384)
                     /locus_tag="AC162A_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HIR5009664.1"
                     /translation="MPTPTTLEIEFGGRTLTLETGKLAGLAGGAVTLRYGDSMVLGTA
                     NRSEPRPGLDFFPLTVDFEERMYAAGKIPGGFIKREGRASEAATLAARLTDRPIRPLF
                     PEGYKDDIQLVITVLSTDHENDPDILGTIAASAALTISEIPFQGPVGSVRVGYIDGEF
                     VLNPTYPQLADSQLDLVVSGTRDAIMMVEAGVKILPEDVMAEAILFGHRSLLPLVELQ
                     EKLRELVGKPKRVPYIEPATDSVLKFAELAKAGGEFVVVDVETTGTDPKMADLIEVAA
                     VRVKGGKITDRWSTFVNPGRPIVGNQMHGITDKDVKGAPSPAEAARKALDFVGNAPFV
                     GHSVGFDIAFLEEALGDGTRVEQGRYLDTLVIARDGYPDLENYKLGTLANFFGVELKE
                     SHRALPDAEATANLLIWFGNDLPGRIDALGEGIATGVRAAKGDAAESAKLIEAARRNA
                     RVSKSLFGLVQ"
     gene            complement(1503..1772)
                     /gene="rpsO"
                     /locus_tag="AC162A_02135"
     CDS             complement(1503..1772)
                     /gene="rpsO"
                     /locus_tag="AC162A_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR5009665.1"
                     /translation="MPLAREKKQEVIGQYAVKDGDTGSPEVQIALLTERIRDLTEHLR
                     RFPKDNHSRRGLLKLVGQRRRLLAYLVKKDATRYRAVIGSLGLRR"
BASE COUNT          253 a          588 c          664 g          315 t
ORIGIN      
        1 tctggacgag cccgaacagg ctcttgctga cgcgcgcgtt gcggcgggcg gcctcgatga
       61 gcttggccga ctcggcggcg tcgcccttcg cggcgcggac cccggtcgcg attccctcgc
      121 ccagcgcgtc gatgcggccg gggaggtcgt ttccgaacca gatcagcagg ttggcggtgg
      181 cctcggcgtc cggcaacgct cggtggctct ccttcagctc gacaccgaag aagttggcca
      241 gcgtgccgag cttgtagttc tcgagatccg ggtagccgtc gcgggcgatg acgagcgtgt
      301 ccaggtacct gccctgctcc acgcgcgtgc cgtcgccgag ggcctcctcg aggaacgcga
      361 tgtcgaagcc gaccgagtgg ccgacgaacg gcgcgttgcc gacgaagtcg agcgccttgc
      421 gggcggcctc ggccgggctc ggcgcgccct tgacgtcctt gtcggtgatg ccgtgcatct
      481 ggttgccgac gatcgggcgc ccggggttga cgaaggtcga ccagcggtcg gtgatcttgc
      541 cgcccttgac gcggaccgcg gcaacctcga tgaggtcggc catcttgggg tcggtgccgg
      601 tggtctcgac gtcgacgacg acgaactcgc cgcccgcctt cgccagctcg gcgaacttga
      661 gcacggagtc cgtcgcgggc tcgatgtacg gcacgcgctt gggcttgccc acgagctcac
      721 gcagcttctc ctgcagctcg acgagcggca gcagcgagcg gtgcccgaac aggatggcct
      781 cggccatgac gtcctcgggc aggatcttca cgcccgcctc gaccatcatg atcgcgtcgc
      841 gggtgcccga gacgacgagg tcgagctggg agtccgcgag ctgcgggtag gtcgggttga
      901 gcacgaactc gccgtcgatg tagccgacgc gcacggagcc gaccgggccc tggaacggga
      961 tctcgctgat cgtcagcgcg gccgacgcgg cgatggtgcc gaggatgtcc gggtcgttct
     1021 cgtggtccgt tgacaggacc gtgatcacga gctggatgtc gtccttgtag ccctcgggga
     1081 acagcgggcg gatcgggcgg tcggtcaggc gcgcggcgag cgtcgccgcc tcggacgcgc
     1141 ggccctcgcg cttgatgaat ccgcccggga tcttgccggc ggcatacatc cgctcttcga
     1201 agtcgaccgt gagcgggaag aagtccagtc cggggcgcgg ttctgaacgg ttggcggtcc
     1261 cgaggaccat cgagtcgccg tagcgaagcg tgacggcgcc gccggcgagg ccggcgagct
     1321 tgccggtctc gagcgtgagg gtccgaccgc cgaactcgat ctcgagcgtg gtgggtgtgg
     1381 gcacgtgtgt acgacctcct tggtcgtgcg tgcgcggatg gatctgtcgt tgtggcgggg
     1441 cgggcgcagg aaccggacac gaacgacccc gggttctcga cccggggtcc gtgtggcgag
     1501 gcctagcggc gcagccccag cgagccgatg acggcccgat agcgcgtggc gtccttcttc
     1561 acgaggtacg cgagcaaacg tcggcgctgt ccaacgagct tgaggaggcc gcggcgcgaa
     1621 tggttgtcct tcgggaagcg gcggaggtgc tccgtcaggt cccggatccg ctccgtcagg
     1681 agagcgatct ggacctccgg ggaaccggtg tcgccgtcct tgacggcgta ctgcccgatc
     1741 acctcttgct tcttttcccg cgcgagcggc actcgatcct ccgacacgaa cgatggcgtg
     1801 actgtattcc ttgttctttc
//
LOCUS       DBNHXO010000104         4019 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4192653, whole genome shotgun sequence.
ACCESSION   DBNHXO010000104 DBNHXO010000000
VERSION     DBNHXO010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4192653"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1974
                     /locus_tag="AC162A_02140"
     CDS             <1..1974
                     /locus_tag="AC162A_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5009666.1"
                     /translation="TTIAMTFTYEDDEGEPLQGRLAKSFRGAKGQVRLELGGDPITDP
                     ARADEELAAISGVPTEGFYRSTASVRHHELAGLQRDEGALRDRLQTSISGADRGMSRA
                     RRKLERAIHDLQTRGIKNPGRLRVAEEAVTVAASSLQAGEDSLGRLERDRDALAVSRE
                     HRAEAEAALAERRSMLEKARQAERLTAERDAAQEKFERYRTAVGLSEEIAELERTHPS
                     PTPLPVLRASVDRLRTVDSKIATLQGMLEGEVRVSYDAPPETRWRLLSRWAIVLVLVG
                     VLIAAVGFLVGFADILAVGILPVLVGGLVAVVGLVLAVVGWWLRRGEPEVESLRDVEV
                     DRRLRGRSEIEQELREAQAEHDDVLQRLGLTEFSEAEERLGLEEAHVAEIDQRRARLS
                     GLIGDETPDVLPARRDNAALEIEQKTAALEALGPIAKEPRARERLEVEVGDAERQVER
                     SRDDEANARARVEQNPVDAEEVAALAERLAIGREALAALRRRERVYERTLAELNTAEQ
                     ATMELATRYLERRMVPDVERITGGRYRRVRVDDSNLSMEVYSPERGDWVPVTELSQGT
                     VDVIYLAARIGLVRLVTGDRRPPIVLDDPFVTLDDGRAARALELLRDLAADFQVIYLT
                     TSDRYDALAERVIVLEGPTAVDAWTEPQGVAPA"
     gene            2003..2833
                     /locus_tag="AC162A_02145"
     CDS             2003..2833
                     /locus_tag="AC162A_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR5009667.1"
                     /translation="MGLISAASWGAGDFGGGMLSRRAPLFGVVFGSQLVGMVAALGIG
                     IVRGEPIPQGADVAWSVVAGFFGVLGITSLYRGLSVGRMGVVAPTTGVLAAVIPVVTG
                     FVTEGLPQTEVIVGIGLALIAVILVTRAPGHADAGPSGIEWGLLGGLGIGLFNVCIGQ
                     LSGAGAFGPLVLVRLVQAGILLVVIVVGRQAWRMPLPAARWVVVVGLLDMAGNAAFIL
                     AAQLGALAIAATLSSLYPVGTVILAVAILHERLTRSHAAGIVLTGIAILLIGLGSAGS
                     "
     gene            2861..3319
                     /locus_tag="AC162A_02150"
     CDS             2861..3319
                     /locus_tag="AC162A_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR5009668.1"
                     /translation="MRISAKVDYAVRAMCELAAHDGATPLKAEQMAAAQEIPLSFLEN
                     ILVDLRRAELVRSLRGQVGGYRLARAAETISIADIIRAVEGPLADVRGTRPEALRFEG
                     PATALRDVWLANRVSVRRVLEQVTIADVVAGTLPADVRALLDDKDALVGH"
     gene            complement(3321..>4019)
                     /locus_tag="AC162A_02155"
     CDS             complement(3321..>4019)
                     /locus_tag="AC162A_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009669.1"
                     /translation="SGRWPTYVLPAPTDVLARLAADVQTPELWTAVMVTLRRAIWGFG
                     IAIVIGVGIGVLVSRFAVMRSAVGSLIMGLQTMPSIAWFPLAVLLFKLTEEAITFVVV
                     LGAAPSIANGLIHGVDHIPPVLTRAGRVLGAKGIDAYRYIILPAAMPSFVAGLKQGWA
                     FAWRSLMAGELIVIIANKTSLGQLLSVNRELSDSQGLLAIMLVILTIGILLDSLVFGT
                     VERRIRAKRGLLTA"
BASE COUNT          530 a         1441 c         1503 g          545 t
ORIGIN      
        1 acgacgatcg cgatgacctt cacctacgag gacgacgagg gcgagccgct ccagggcagg
       61 ctcgcgaagt cgttccgcgg cgccaagggg caggttcgcc tcgagctggg cggcgacccg
      121 atcacggacc cggcgcgcgc cgacgaggag ctggcggcga tctcgggcgt gccgaccgag
      181 ggcttctacc ggtcgacggc gtcggtccgc caccacgagc tcgcgggcct ccagcgcgac
      241 gagggcgccc tccgcgaccg cctccagacg tcgatcagcg gcgccgaccg cggcatgagc
      301 cgcgcccgac gcaagctcga gcgggccatc cacgacctcc agacccgggg aatcaagaac
      361 cccggccgcc tgcgggtcgc cgaggaggcg gtgacggtcg ccgcctcgag cctccaggcg
      421 ggcgaggact cgctggggcg gctcgagcgg gaccgcgacg cgctggcggt ctcccgcgag
      481 caccgggccg aggcggaggc cgcgctcgcg gagcgccgct cgatgctcga gaaggcgcgc
      541 caggccgagc ggctgaccgc ggagcgcgac gcggcccagg agaagttcga gcgctatcgg
      601 acggccgtcg gactgagcga ggagatcgcc gagctcgagc gcacccaccc gtcacccacg
      661 ccgctccccg tcctgcgcgc gtccgtcgat cggctgcgaa cggtggactc caagatcgcc
      721 acgctccagg gcatgctcga gggcgaggtg cgggtgagct acgacgcgcc gccggagacc
      781 cgctggcgcc tgctctcgcg ctgggccatc gtgctcgtgc tcgtcggcgt cctgatcgcc
      841 gccgtcggct tcctcgtcgg gttcgccgac atcctcgcgg ttggcatcct ccccgtcctc
      901 gtcggtgggc tggtcgccgt ggtcggcttg gtgctggccg tagtcggctg gtggctccgg
      961 cgcggcgagc ccgaggtgga gtcgctgcgc gacgtcgagg tcgaccgccg gctgcgtggc
     1021 cggtccgaga tcgagcagga gctgcgcgag gcccaggcgg agcacgacga cgtgctgcag
     1081 cgcctcggcc tgaccgagtt ctccgaggcc gaggagcgcc tcggcctgga ggaggctcac
     1141 gtcgccgaga tcgaccagcg gcgtgcgcgg ctgtcgggcc tgatcggcga cgagacgccc
     1201 gacgtgctgc cggcccggcg cgacaacgcg gccctcgaga tcgagcagaa gacggcggcg
     1261 ctggaggccc tcgggccgat cgccaaggag ccgcgggcgc gagagcggct cgaggtcgag
     1321 gtcggcgacg ccgagcgcca ggtcgagcgc agccgcgacg acgaggccaa cgcgcgggca
     1381 cgggtcgagc agaaccccgt cgacgccgag gaggtcgccg ccctcgccga gcggctcgcc
     1441 atcggtcgcg aggcgctggc cgcgctccgt cgccgggagc gggtctacga gcggacgctg
     1501 gccgagctca acaccgccga gcaggcgacg atggagctcg cgacccggta cctcgagcgg
     1561 cgcatggtgc ccgacgtcga gcgcatcacc ggcgggcgct accgccgggt ccgggtcgac
     1621 gacagcaacc tctcgatgga ggtctattcg cccgagcgcg gcgactgggt cccggtgacg
     1681 gagctctcgc agggcaccgt cgacgtgatc tacctcgccg cgcggatcgg gctagtccgc
     1741 ctggtcaccg gcgaccgccg cccgccgatc gtgctcgacg acccgttcgt gaccctcgac
     1801 gatggccgcg ccgcccgcgc cctggagctg ctgcgcgacc tcgccgcgga cttccaggtg
     1861 atctacctca cgacgtcgga ccgctacgac gcgctggccg agcgggtcat cgtcctcgag
     1921 ggcccgacgg ccgtcgacgc ctggaccgag ccgcaggggg tggcccctgc ttgaggtcac
     1981 gggaccgttc gcggtcctgg cgctgggcct catctcggcg gccagctggg gggccggcga
     2041 cttcggcggc ggaatgctca gccggcgtgc gccgctgttc ggcgtcgtgt tcggctcgca
     2101 gctcgtgggg atggtcgcgg cgctcgggat cggcatcgtc cgcggcgagc ccatccccca
     2161 gggtgcggac gtcgcgtggt cggtcgtcgc cggcttcttc ggggtcctcg ggatcacctc
     2221 gctctaccgt ggcctgtccg tcggccgcat gggggtcgtc gcgccgacga cgggcgtgct
     2281 cgcggcggtg atccccgtcg tcaccgggtt cgtgaccgag ggcctgccgc agaccgaggt
     2341 catcgtgggc atcgggctcg cgctgatcgc cgtcatcctc gtcacccggg cacccggcca
     2401 cgccgacgcc ggcccgtcgg gcatcgagtg ggggctgctc ggcggcctcg ggatcggcct
     2461 gttcaacgtg tgcatcgggc agctgtcggg cgccggcgcg ttcggcccgc tggtcctcgt
     2521 ccggctggtc caggccggga tcctgctggt ggtgatcgtc gtcgggcggc aggcgtggcg
     2581 gatgccgctc cccgcggcgc gttgggtggt cgtcgtgggc ctgctcgaca tggctggcaa
     2641 cgcggcgttc atcctcgccg cgcagctggg cgcgctcgcg atcgccgcca cgctgtcctc
     2701 gctctacccg gtgggcacgg tgattctcgc cgtcgcgatt ctccacgagc ggctgacgcg
     2761 gagccacgcc gcggggatcg tcctgacggg gatcgcgatc ctgctcatcg ggctcggctc
     2821 cgccgggtcg tagcggcctg cgcccgcgta cgatcagccc gtgaggatct cggccaaggt
     2881 cgactacgcc gtccgcgcga tgtgcgagct cgcggcgcac gacggcgcga cgccactcaa
     2941 ggcggagcag atggccgcgg ctcaggagat cccgctctcg ttcctcgaga acatcctggt
     3001 cgacctgcgg cgcgccgagc tcgtgcgctc gctccgcggg caggtcggcg gctaccgcct
     3061 ggctcgtgcc gccgagacga tctcgatcgc cgacatcatc cgcgccgtcg agggcccgct
     3121 cgcggacgtc cgcgggacgc gccccgaggc gctgcggttc gagggcccgg ccacggcgct
     3181 ccgtgacgtc tggctggcga accgggtgag cgtgcggcgt gtgctcgagc aggtgacgat
     3241 cgccgacgtc gtggcgggca ccctgccggc cgacgtccgc gccctgctcg acgacaagga
     3301 cgcgctcgtc ggccactgac tcaggcggtc agcaggcccc gcttggcccg gatccgccgc
     3361 tcgaccgtcc cgaacacgag cgaatcgagc aggatcccga tcgtcaggat cacgagcatg
     3421 atcgcgagca ggccctggct gtcggaaagc tcgcggttga ccgagagcag ctggccgagg
     3481 gaagtcttgt tcgcgatgat cacgatcagc tcgcccgcca tcaggctccg ccacgcgaag
     3541 gcccagccct gcttgaggcc ggccacgaag ctcggcatgg ccgcgggcag gatgatgtac
     3601 cggtaggcgt cgatgccctt ggcgccgagc acccgtccgg cccgggtgag caccggcggg
     3661 atgtggtcga cgccgtggat caggccgttc gcgatcgagg gcgccgcgcc gagcacgacc
     3721 acgaacgtga tcgcttcctc ggtgagcttg aacaggagca ccgcgagcgg gaaccacgcg
     3781 atcgagggca tggtctggag gcccatgatc agcgagccga cggcggagcg catcaccgcg
     3841 aaccgcgaca ccagcacgcc gatcccgacg ccgatcacga tggcgatgcc gaagccccag
     3901 atcgcccggc gcaacgtgac catgacggcg gtccagagct cgggcgtctg cacgtcggcg
     3961 gcgaggcggg cgaggacgtc agtcggcgcc ggcaggacgt acgtcggcca gcggccgct
//
LOCUS       DBNHXO010000105         4069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4589880, whole genome shotgun sequence.
ACCESSION   DBNHXO010000105 DBNHXO010000000
VERSION     DBNHXO010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4589880"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1092
                     /locus_tag="AC162A_02160"
     CDS             256..1092
                     /locus_tag="AC162A_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HIR5009670.1"
                     /translation="MDTRVAIPTGDRQYHIGLGPGELADYILLPGDQDRVDRVAAHFD
                     SVEFRHRHREFATATGMFRGLRVSCLSTGIGTDNVEIVISEVLALNPHPTFIRIGSCG
                     ALQEGIGLGDLVISTGSVRLETTTNWFVSDGYPAVASYEAVLALEEAATRLGHPHHVG
                     ITATAPGFFGAQGRPIPQLPIRYPDLADEMSRQGILNFEMEASAVLVLAGLGGGRAGV
                     VCAVYANRRTGEFVTGDHKDRAEAAVVETGLESLLVLAELDRQKAKAGAARWRPSLWA
                     PQ"
     gene            1169..1309
                     /locus_tag="AC162A_02165"
     CDS             1169..1309
                     /locus_tag="AC162A_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018194239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HIR5009671.1"
                     /translation="MAQAYCVKDKMKVEVQNPQQITMKNGKPALSGTCPKCGTKVFKI
                     GG"
     gene            1780..2805
                     /gene="gap"
                     /locus_tag="AC162A_02170"
     CDS             1780..2805
                     /gene="gap"
                     /locus_tag="AC162A_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR5009672.1"
                     /translation="MTVRVGINGFGRIGRQSLKAIIERAPGVEVVAVNDLVDVPTNAL
                     LFKHDSTYGAYPGEVSHTDDAVVIDGKAIKVLQVKDPAQLPWGDLGVDIVIESTGLFT
                     DAEKARAHINAGAKKVIISAPAKGEDITIVLGVNEGQYDPDRHQIISNASCTTNCLAP
                     AAKVVHDKFTIRRGLMNTIHSYTNDQRILDVAHKDPRRARAAGQNIIPTSTGAAKALA
                     LVMPDLKGKFDGFSLRVPTPTVSVVDFTAELERETTIDELNQAFRDAAAGPLKGILGA
                     SDEPLVSTDFRGDSRSSIVDLPSNMVLGGNFIKVIAWYDNEWGYSCRVADLAQFVAER
                     LPAVATV"
     gene            2854..4062
                     /locus_tag="AC162A_02175"
     CDS             2854..4062
                     /locus_tag="AC162A_02175"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR5009673.1"
                     /translation="MDKLTVRDLDASEKRVFVRVDFNVPLADGRVTDDSRIRAALPTI
                     RYLQGQGARVILASHLGRPDGKVTDSLRLRPVAERLGQLMGKTVPVTGDALGLGTEDA
                     VRRMRAGEILLLENLRFHVEEEKNDPVFAEALASYCDVYVNDAFGTAHRAHASTVGIA
                     RLRPAYAGLLMEKELANLGKLLDNPERPFAAIIGGAKVSDKIKVLEHLLDKVDAMVIG
                     GGMANTFMLAQGKSVGKSLAEPDRVDDARAILAAAERQGVTVVLPVDVVIAKEVTRGT
                     EYKTIPAEKVPASWHIVDLGKQSLANIEEALANVRTVLWNGPLGVFEIPSFAHGTNAI
                     ARFLAKRAEQGAAVVIGGGDSVAAIQQQGLADKMTHISTGGGASLEFLEGRELPGVTV
                     LLDRQPEAVK"
BASE COUNT          667 a         1458 c         1360 g          584 t
ORIGIN      
        1 gcccgccgcg agcgctcacg gcgggtcctg cggtcgcgca tcgtggcccg tctggaggcc
       61 ttcgggcggg gcggcgaagg gtccggatcg gcccccggtc ggtcagccga gcccccgttg
      121 ggggcgcaaa ccgggccgga actcggcgga aacgtgcgtg gatcggcttg acgctgcacg
      181 aatcaggccc ctatgctcga tcgcaggcgc acgcaaaccg agcgtggccc atgcgggagc
      241 gagcctttgc cgaacgtgga tacccgggtc gcgatcccta ccggcgaccg gcaataccac
      301 atcggcctgg ggccaggcga gctggccgac tacatcctcc tccccggcga tcaggatcgc
      361 gtcgatcgcg tcgccgccca ctttgactcc gtcgagttcc ggcatcggca ccgcgagttc
      421 gcgaccgcca ccggtatgtt caggggcctg cgggtgtcgt gcctctcgac cgggatcggc
      481 accgacaacg tcgagatcgt gatcagcgag gtcctggcgc tcaacccgca ccccacgttc
      541 atccgcattg gatcgtgcgg cgccctgcag gaggggatcg gactcggcga cctcgtgatc
      601 tcgaccggat cggtccgcct ggagaccacg acgaactggt tcgtgtccga cggctacccg
      661 gccgtggcga gctacgaggc ggtgctcgcg ctcgaggagg ctgccacgcg gctgggtcat
      721 ccccatcatg tcgggattac cgccacggcg cccggattct tcggtgcgca gggacgcccg
      781 atcccgcagc ttcccatccg ctaccctgac ctcgccgacg agatgtcccg tcagggcatc
      841 ctgaactttg agatggaagc ctctgcggtg ctcgtgctcg caggtctcgg tggtggacgt
      901 gccggcgtcg tgtgcgccgt ctacgcgaac cgtcggaccg gtgagttcgt caccggcgac
      961 cacaaggacc gggcggaagc tgccgtcgtg gagacggggc tggaaagcct cctggtcctc
     1021 gcggagctgg accgtcagaa ggcgaaggct ggagcggcgc gctggcgccc gtcgctctgg
     1081 gccccacaat gagcgccaga ccgagcgaac ctgcggaccc gagaggcgtg ctctcgcgac
     1141 gcggatagcg tgaggaggag cggactcgat ggctcaggcc tactgcgtca aggacaagat
     1201 gaaggtcgag gtccagaacc cgcagcagat cacgatgaag aacggcaagc cggcgctctc
     1261 gggcacctgc ccgaagtgcg gcaccaaggt gttcaagatc ggcggctgac gccggcacat
     1321 cgttgagcga ccagagccct ccggcaggac cggagggctc tttcgtcatc agcgcacggt
     1381 gcactcggcc cccctgattc ggggcggtcg gcgcgggtcc tcggacggcc ctttgctagg
     1441 attgaccgag aacccctcga tccgctgttc ccgaggtcac gccctgttcg ctttcatggc
     1501 atccgccacc gacctcggct ccaggcacca tcgatcgagg atggacatcc acgagcaccg
     1561 gtcactcgct cggcctgccg acgtcggcac gccgcccggc ctcggggtcg ccgcagccgt
     1621 cgccacgatc gcgaccgccg cagcgctccc gacgtcgttc cggccggtcc tcggatcgac
     1681 gcgagaccgc gtccgtcccg cggttcgcct gtctcccgcc acggtcgccg cgtcgcgcgg
     1741 actcgatcgt gcgcccttcc gatccaagga ggatctccga tgaccgtccg cgtcggtatc
     1801 aacggtttcg gccgcatcgg ccgccagtcg ctcaaggcca tcatcgaacg cgccccgggc
     1861 gtcgaggtgg tcgccgtcaa cgacctcgtc gacgttccga ccaacgcgct gctgttcaag
     1921 cacgactcga cgtacggcgc ctacccgggc gaggtgagcc acactgacga cgcggtcgtc
     1981 atcgacggca aggcgatcaa ggtgctccag gtcaaggacc ccgcccagct cccgtggggc
     2041 gacctcgggg tcgacatcgt catcgagtcg accggcctgt tcaccgatgc cgagaaggcc
     2101 cgcgcccaca tcaatgccgg cgcgaagaag gtgatcatca gcgcccccgc caagggcgag
     2161 gacatcacga tcgtcctggg cgtcaacgag ggccagtacg accccgaccg gcaccagatc
     2221 atcagcaacg cgagctgcac gacgaactgc ctcgccccgg cggcgaaggt cgtccacgac
     2281 aagttcacga tccggcgcgg cctcatgaac acgatccact cgtacaccaa cgaccagcgc
     2341 atcctcgacg tcgcccacaa ggatccgcgc cgcgcgcgcg ccgccggcca gaacatcatc
     2401 ccgacctcga ccggcgcggc caaggccctc gccctcgtga tgcccgacct caagggcaag
     2461 ttcgacggct tcagcctgcg cgtcccgacg ccgaccgtgt ccgtcgtcga cttcaccgcc
     2521 gagctcgagc gcgagacgac catcgacgag ctcaaccagg cgttccgcga cgccgccgcc
     2581 ggcccgctca agggcatcct cggcgcatcg gacgagccgc tggtgtcgac cgacttccgc
     2641 ggcgactccc gctcctcgat cgtcgacctg ccgtcgaaca tggtcctggg cggcaacttc
     2701 atcaaggtca tcgcctggta cgacaacgag tggggctaca gctgccgcgt ggctgacctc
     2761 gcgcagttcg tcgcggagcg cctgccggcg gtcgccacgg tctgaccgga gcccgaggct
     2821 cccagctcgg acgcttccgg aggaacaccg accatggaca agctcaccgt ccgggacctg
     2881 gacgcgtccg agaagcgcgt cttcgtccgc gtggacttca acgtgccgct ggccgatggc
     2941 cgggtcaccg acgattcgcg catccgcgcc gcgctgccca cgatccggta tctccagggc
     3001 cagggtgccc gggtgatcct cgccagccac ctcggccggc ccgacggcaa ggtcaccgac
     3061 agcctccggc tccggccggt ggccgagcgg ctcggccagc tgatgggcaa gaccgtgccg
     3121 gtcacggggg atgcgctcgg cctcgggacc gaggacgcgg tccgccggat gcgcgccggc
     3181 gagatcctgc tgctcgagaa cctccgcttc catgtcgagg aggagaagaa cgacccggtg
     3241 ttcgccgagg ccctggcctc gtactgcgac gtctacgtca acgacgcgtt cggcaccgcg
     3301 caccgggcgc acgccagcac ggtcggcatt gccaggctgc ggccggccta cgccggcctg
     3361 ctgatggaga aggagctcgc caacctcggc aagctgctcg acaacccgga gcggccgttc
     3421 gccgcgatca tcggcggcgc caaggtcagc gacaagatca aggtgctcga gcacctgctg
     3481 gacaaggtcg acgccatggt gatcggcggc ggcatggcga acaccttcat gctcgcccag
     3541 ggcaagtcgg tcgggaagag cctcgcggag cccgatcgcg tcgacgacgc gcgcgcgatc
     3601 ctcgcggcgg ccgagcgcca gggcgtcacc gtcgtgctgc cggtcgacgt ggtgatcgcc
     3661 aaggaggtca cccgcggcac cgagtacaag acgatcccgg ccgagaaggt gccggcgagc
     3721 tggcacatcg tcgacctcgg caagcagagc ctcgcgaaca tcgaggaggc gctggccaac
     3781 gtgcgaaccg tgctctggaa cggaccgctg ggcgtgttcg agatcccctc gttcgcgcac
     3841 ggcaccaacg ccatcgcccg gttcctggcg aagcgcgccg agcagggcgc cgcggtcgtg
     3901 atcggcggcg gcgactcggt cgcggcgatc cagcagcagg gcctggccga caagatgacc
     3961 cacatcagca ccggcggcgg cgcgtcgctg gaattcctcg agggacgcga gctgccgggc
     4021 gtgacggtgc tcctggaccg tcagccggag gccgtcaagt gagcgatac
//
LOCUS       DBNHXO010000106         4128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_621046, whole genome shotgun sequence.
ACCESSION   DBNHXO010000106 DBNHXO010000000
VERSION     DBNHXO010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_621046"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="AC162A_02180"
     CDS             <1..726
                     /locus_tag="AC162A_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009674.1"
                     /translation="YFYMIGMIAEPVVYLVVWTTVANQQGGSVAGITSGEFAAYYIVW
                     TLVRNMNVVFTPFGWEERIREGQFSGLLLRPVHPIHWDLGYFAGWKVVVIVLWLPIAY
                     VLSLLFHPTAHITPLGVIVFLISIWGAYLIRSLNQSTLGMVTFWTTRVGPVFSLYFAA
                     ELLLSGRLVPPQLLPEWAQRLASILPFQYTFAFPIEALIGDLPPETLLAGLVMQGLWI
                     IIGAALVRRSFTIAVRRYSAVGG"
     gene            726..1511
                     /locus_tag="AC162A_02185"
     CDS             726..1511
                     /locus_tag="AC162A_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009675.1"
                     /translation="MRAFRLFWTFARVGALNELQYRANLAIQIVESAITLATGLVVLA
                     LVFNQVDELNGWSHAELLAVMGVYTLVGGIIGAFIQPNMVRLIGDVRQGTLDYILTKP
                     DDSQVLVSVREFRIWHLVDVVLGIVILVIAAREVPGTLGPAGTAAFLFALALGAAMVY
                     CFWLILTTAAFWIVRMDEIVDLFAGVYQAGRWPVSIYPDWLRVGLTFLVPIAFAVTVP
                     AEAFTQRLEPATLGLAALFAVGLLVITRLLWRRGVRRYSGASA"
     gene            complement(1531..2136)
                     /locus_tag="AC162A_02190"
     CDS             complement(1531..2136)
                     /locus_tag="AC162A_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5009676.1"
                     /translation="MEPVRGRPQAGLDLDVPDAEPIEPPVVALRRTVDPADWVEAAYE
                     EFRTEIFSFAMRGTREVAAAEDATQEAFIRLLREARENRIPDDPRAWLYRVVSNLLIS
                     GGRRATVAQRWRGVLARRETPSQPSPEATAVRRERRDAVQIALDAMSPDARTALLLAA
                     NGFNGREIANQLGRTELATRALLCRARTHLRTTLEAPDGSD"
     gene            complement(2228..3805)
                     /locus_tag="AC162A_02195"
     CDS             complement(2228..3805)
                     /locus_tag="AC162A_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR5009677.1"
                     /translation="MQQLPEVRVKTAVHTGPLTHDRARELATNAIDFDLSTAEWEQLD
                     GHLASCEPCRRFAAATRRDQARLAALPQREPPPRIHEALRRAEATRRDRTRPLLVLAA
                     AGMAGALAFATLGGGAQPKPADSPAVSTPEASIALATAAPTPAASAPAATTEPPSASP
                     TEPPRLPVDWAAVETQPSFHDPFGVMEAVAAGQRLVAVGDGCSVSLPQCHVAIWTSED
                     GETWERVPDDPKFHAGPYTPARRGELTDVVATDAGFLAVGRSREGDTRRAVVFWSNDG
                     LRWTRSRDDASLLLGTFEAAVETPSGFVAVGNVLEDRIAHASAWMSPDGLAWVRTTGD
                     GAAFAVGNGGTYSDGRMNGGMIDVVWTGTRIVAIGSACSLDGTGCRGVAWTSGRGQAW
                     ARQDGDLTGTPYSMVATGEGLVAVGDDGAGHPLSWISADDGDTWLPGRPVSGYLGNFS
                     AVVATPSGLVATIATTDLTSAVATSTDGLSWQIVADPGALGQGVVNGLAWDPVRSLAI
                     GVGWDGQTPAAAVWLGR"
     gene            3850..>4128
                     /locus_tag="AC162A_02200"
     CDS             3850..>4128
                     /locus_tag="AC162A_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_02809 family neutral zinc metallopeptidase"
                     /protein_id="HIR5009678.1"
                     /translation="MRWSRGHTGSIEDRRSQGGGGGGLGGGMGGIPIPASVGGGGALL
                     IVVAIIVLNLLSSSGGGGAGSGGLGGVLDGLGGGAVGPVGTPDLTNDGD"
BASE COUNT          526 a         1453 c         1502 g          647 t
ORIGIN      
        1 tacttctaca tgatcggcat gatcgccgag ccggtcgtct acctcgtcgt ctggacgacc
       61 gtcgccaacc agcagggcgg ctcggtcgcg gggatcacgt ccggcgagtt cgccgcctac
      121 tacatcgtgt ggaccctcgt ccggaacatg aacgtggtgt tcacgccctt cgggtgggag
      181 gagcggatcc gcgagggcca gttctccggc ctcctcctgc ggccggtcca cccgatccac
      241 tgggacctcg gctacttcgc cggctggaag gtcgtcgtca tcgtcctgtg gctgccgatc
      301 gcgtacgtgc tgtcgctgct gttccacccg acggcgcaca tcacgccgct gggcgtgatc
      361 gtgttcctga tctcgatctg gggcgcctac ctgatccggt cgctcaacca gtcgaccctc
      421 gggatggtga cgttctggac gacccgcgtc ggcccggtgt tcagcctcta cttcgccgcc
      481 gagctgctgc tctcagggcg gctcgtgccg ccgcagctgc tgcctgagtg ggcgcagcgg
      541 ttggcgtcga tcctgccgtt ccagtacacg ttcgcgttcc cgatcgaggc gctgatcggc
      601 gacctgccgc ccgagaccct gctggcgggc ctcgtgatgc agggcctgtg gatcatcatc
      661 ggtgccgcgc tggtccggcg atcgttcacg atcgccgtcc ggcgctactc ggcggtgggc
      721 ggctgatgcg cgcgttccgg ctgttctgga ccttcgcccg cgtcggcgcg ctcaacgagc
      781 tccagtaccg ggccaacctc gccatccaga tcgtggagtc ggcgatcacc ctcgcgaccg
      841 gcctcgtggt cctcgccctc gtgttcaacc aggtcgacga gctcaacggc tggtcccacg
      901 cggagctgct cgccgtgatg ggcgtgtaca cgctcgtcgg cggcatcatc ggcgcgttca
      961 tccagcccaa catggtgcgc ctgatcggcg acgtacggca ggggacgctg gactacatcc
     1021 tcaccaagcc cgacgacagc caggtgctcg tcagcgtccg cgagttccgg atctggcatc
     1081 tcgtcgacgt ggtgctcggg atcgtgatcc tggtgatcgc ggcacgcgag gtgcccggga
     1141 cgctcgggcc ggcggggacc gcggcgttcc tgttcgccct ggcgctgggg gcggcgatgg
     1201 tctactgctt ctggctcatt ctcacgaccg ccgcgttctg gatcgtgcgc atggacgaga
     1261 tcgtcgacct gttcgcgggc gtctaccagg ccggccgctg gcccgtctcg atctaccccg
     1321 actggctccg cgtcggcctc accttcctcg tcccgatcgc gttcgccgtg accgtgcctg
     1381 cggaggcgtt tacgcagcgc ctggagcccg cgactctcgg gctggccgcg ctgttcgcgg
     1441 tcgggctgct ggtgatcacg cgactgctgt ggcgccgggg cgtccggcgg tattcgggcg
     1501 cgtcggcgta ggcgctgggc gccagccagc ctagtcgctc ccgtcgggcg cctcgagcgt
     1561 ggtgcggagg tgcgtccggg cccggcacaa cagggcgcgc gtcgcgagct ccgtgcgtcc
     1621 aagctgattg gcgatctccc ggccgttgaa cccgttcgcc gccagcagga gcgcggtccg
     1681 cgcgtcgggg ctcatggcat cgagcgcgat ctggacggcg tcccgccgct cgcggcggac
     1741 cgccgtggcc tcgggcgacg gctgggatgg tgtctcgcgg cgcgccagga ccccccgcca
     1801 gcgctgggcg acggtcgcgc gacggccgcc gctgatcagg aggttggaca cgacccggta
     1861 gagccacgcc cgcggatcgt ccgggatacg gttctcgcgc gcctcgcgca gcaggcggat
     1921 gaacgcctcc tgggtcgcgt cctccgccgc cgcgacctcg cgcgtgccgc gcatcgcgaa
     1981 cgagaagatc tcggtgcgga actcctcgta cgccgcttcg acccagtcgg cggggtcgac
     2041 ggtccggcgc agggcgacca ccggcggctc gatcggttcc gcgtccggaa cgtcgagatc
     2101 cagcccggcc tgcggtcggc ctcgaaccgg ctccatccaa tggtccctca cgcgcggcac
     2161 cggccgcggt accagaaccc gcgcggtgcc ctcagcggag gtcattgacg ttcggctgtg
     2221 gaggggttca tcgccccagc catacagccg ccgccggagt ctggccgtcc cagcctactc
     2281 cgatggccaa gcttcgaaca gggtcccagg caaggccgtt gacgacgccc tgaccaagcg
     2341 cgcccgggtc ggcaacgatt tgccacgaca ggccatccgt ggaggtcgcc acggcggagg
     2401 tgaggtcggt cgtcgcgatg gtcgccacga ggccgctcgg cgtcgctacg accgcgctga
     2461 agttgccgag gtaaccggaa acgggccggc ccggcagcca ggtgtcgccg tcgtcggccg
     2521 agatccagct cagcgggtgg ccggcgccat cgtcaccgac ggccacgagg ccctcgccag
     2581 tcgcgaccat cgagtagggc gtgccggtca ggtcgccgtc ctgtcgcgcc catgcctgcc
     2641 cgcggccgga cgtccaggcg acaccgcggc acccggtgcc gtcgagggag cacgcgctgc
     2701 cgatggcgac aatgcgggtc ccggtccaga cgacatcgat catcccgccg ttcatccggc
     2761 catcggaata ggtgccgccg ttgccgaccg caaaggccgc accgtcgccg gtggtcctga
     2821 cccaggcgag cccgtcgggc gacatccatg cgctggcgtg cgcgatcctg tcctcgagga
     2881 cgttcccgac agcgacgaag ccgctgggcg tctcgaccgc cgcctcgaag gtgcccaaca
     2941 ggaggctcgc gtcgtcccgg gatcgcgtcc agcgcaggcc gtcgttcgac cagaacacca
     3001 cggcgcgccg ggtgtcgccc tcgcgtgagc ggccgacggc gaggaagccg gcgtccgtcg
     3061 ccacgacgtc cgtgagctcg ccacgacgcg ccggggtata tgggccggcg tggaacttgg
     3121 gatcgtcggg gacgcgctcc caggtctcgc cgtcttcgga cgtccagatc gcgacgtgac
     3181 actgcggcag gctcacgctg cagccgtcgc caaccgcgac gaggcgctgg cctgccgcca
     3241 cagcctccat gacgccgaag ggatcgtgga acgacggctg ggtctccacg gcggcccagt
     3301 cgacgggcag ccgcggcggt tcggtcggcg acgcgctcgg cggctcggtg gtcgcagccg
     3361 gggcggaggc cgccggggtt ggggccgccg tggcgagcgc gatcgaggct tccggggtgg
     3421 acacggccgg gctgtccgcg ggcttgggct gtgcgccacc gccgagggtc gcgaaggcca
     3481 gggcgccggc catccccgcc gccgcgagca ccaggagcgg ccgggtccgg tcgcgccgag
     3541 tggcctcggc tcgcctgagg gcctcgtgaa tcctcggcgg cggctcgcgc tgggggaggg
     3601 cggcgaggcg ggcctgatcg cgcctggtcg cggccgcgaa gcgccggcag ggctcgcagg
     3661 aggcgagatg cccgtcgagc tgctcccact cggccgtcga gaggtcgaag tcgatcgcat
     3721 tggtggcgag ctcgcgcgct cgatcgtgcg tgaggggtcc ggtgtgcacg gcggtcttga
     3781 cgcgaacctc cggaagttgt tgcacccgga tttacgatat tgcaacgccc caccgggcat
     3841 gatcgctcca tgcgctggag ccgggggcac acggggagca tcgaggatcg ccgaagccag
     3901 gggggcgggg gaggcggcct gggcggcggc atgggcggca tccccatccc cgccagcgtt
     3961 ggtgggggtg gcgcgctgct gatcgtcgtc gccatcatcg tcctcaacct gctgagcagc
     4021 agcggcggcg ggggcgcggg gagcgggggg ctcggcggcg tcctcgacgg gctcggggga
     4081 ggagccgtgg gaccggtcgg gacgcccgac ctgaccaacg acggcgac
//
LOCUS       DBNHXO010000107         2573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3597907, whole genome shotgun sequence.
ACCESSION   DBNHXO010000107 DBNHXO010000000
VERSION     DBNHXO010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3597907"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..>2573
                     /locus_tag="AC162A_02205"
     CDS             179..>2573
                     /locus_tag="AC162A_02205"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR5009679.1"
                     /translation="MLDADRGAGGRGPNARVVDPAGTGWADAGRRLLRRVPAPVRVAL
                     FSVFLGGLAVALYVGFLSHQVPLVPPVEIPWYVMAVAFFAAELKVVDVHFRREKHSFS
                     LSEFPAVIGFFLLSPTDYFLAMLVGTGAALVIHNQAPVKMAFNLANFAFTAAVGLSVF
                     YALQTTTGVPDPDDWAAAFAATSVAAVISAASIATAISMSGGAPQYEKLPEMIQFGGL
                     VAIANTSLALLAVSVLWLDPLLLALLIVPLLTVFLAYRAYISEREKHERLELLYQSSR
                     ILQHSPELDSALAALLEHAREMFRAERAEVLLWPRDDSVSEGLLTVCEQDHAPKMMVP
                     APLADADRLHRRVAHERRAFLHISPERHDPAVRNAMVAPLLGEAGLIGSMVVANRLTE
                     GTTFSDDDLRLLETLANQAAVALENGQLEQSLAELSRLKEQLRFQAYHDPLTGLANRS
                     LFAEQVNLALEGPRMGRVPVVLFLDLDNFKDVNDALGHPAGDRLLTAVAERVGSCIRT
                     GDMAARLGGDEFAVLLLDDPELGAAVTVATRLIAAFAVTFPISGQDLKVSASIGIAAS
                     IGTTEHADDMLRNADVAMYTAKQAGKNRFAVFDPTMHAAIVARHAMSTELSHAVTEGE
                     IDVFYQPVLSLATGLTFGVEALARWKHPTRGFVEPDEFIPLAEESGTILALGRAVLVE
                     ACREAAPWRSGTGEGVSLTVNLSAAQLAQETFIDDLVDILRATGFSASRLVLEMTETV
                     MFSDTQTTIARLTALRDLGVRIAVDDFGTGYSSLGYLRRFNVDILKIAREFIAPPESG
                     "
BASE COUNT          345 a          948 c          870 g          410 t
ORIGIN      
        1 tgtgcggcgg ttcggggtgg agggtaccag cgcgggcgac atcgccccgg tcgtcgggcc
       61 gacggagagg tccgggtggc gaaggtactt tcaggtcgcg gttcaccgag gcaccggggc
      121 gtagaaacag gtcatccggt ggcgtccgct cgaaggccgc tgccgcacgg agtgtcagat
      181 gctcgacgcg gaccggggcg ccggaggccg gggccccaac gctcgcgtcg tcgaccctgc
      241 gggcacgggt tgggccgacg cgggccggag gttgctgcgc cgcgtgccgg cgccggtccg
      301 ggtcgccctg ttctcggtgt tcctcggcgg cctcgcggtc gcgctctacg tcgggttcct
      361 cagtcaccag gtcccgctcg tgccgcccgt cgagatcccg tggtacgtga tggcggtggc
      421 gttcttcgcg gccgagctca aggtcgtcga cgtccacttc cggcgcgaga agcactcgtt
      481 ctcgctcagc gagttcccgg ccgtgatcgg gttcttcctg ctgtcgccca ccgactactt
      541 cctggcgatg ctcgtcggga ccggggcggc gctcgtcatc cataaccagg cgccggtgaa
      601 gatggccttc aacctggcca acttcgcgtt cacggcggcg gtgggcctgt ccgtgttcta
      661 cgccctgcag accacgaccg gcgtgcccga tccggacgac tgggcggcgg cgttcgctgc
      721 cacgtccgtc gccgcggtca tcagcgcggc gtcgatcgcg accgccatct ccatgtccgg
      781 cggggcgccc cagtacgaga agctgccgga gatgatccag ttcggcggcc tggtcgcgat
      841 cgccaacacc agcctcgcgc tgctcgccgt gtcggtgctg tggctcgacc cgctgctgct
      901 cgcgctgctg atcgtcccgt tgctcaccgt gttcctcgcc taccgggcat acatctccga
      961 gcgcgagaag cacgagcgcc tcgagctgct ctaccagtcc agccggatcc tccagcactc
     1021 gcccgaactc gactcggcgc tggccgccct gctcgagcac gcccgcgaga tgttccgcgc
     1081 cgagcgcgcc gaggtgctgc tgtggccgcg cgacgactcg gtctcggagg gcctgctgac
     1141 ggtgtgcgag caggaccacg cgcccaagat gatggtcccg gcgcccctcg cggacgcgga
     1201 ccgtctccac cggcgggtcg cccacgagcg acgcgcgttc ctgcacatct ctcccgagcg
     1261 ccacgacccc gccgttcgca acgccatggt cgcgcccctc ctcggcgagg ccgggctgat
     1321 cggctcgatg gtggtcgcca accgcctgac cgagggcacg acgttctccg acgacgacct
     1381 gcgcctgctc gagaccctcg ccaaccaggc ggcggtggcg ctcgagaacg gccagctgga
     1441 gcaatccctc gccgagctct cgcggctcaa ggagcagctc cggttccagg cctaccacga
     1501 ccccctcacg ggcctcgcca accgcagcct gttcgccgag caggtcaacc tcgccctcga
     1561 ggggccgcgg atgggccggg tgccggtcgt cctgttcctc gacctcgaca acttcaagga
     1621 cgtcaacgac gcgctcggcc accccgccgg cgatcgcctg ctgaccgccg tcgccgagcg
     1681 ggtcgggtcg tgcatccgga ccggagacat ggctgcgcgg ctcggtggcg acgagttcgc
     1741 ggtgctgttg ctcgacgacc ccgagctcgg tgccgcggtc accgtcgcga cccggctgat
     1801 cgccgcgttc gccgtcacgt tccccatcag cgggcaggac ctgaaggtgt ccgcgagcat
     1861 cgggatcgcc gccagcatcg gcaccaccga gcacgccgat gacatgctgc gtaacgccga
     1921 cgtcgcgatg tacacggcca agcaggccgg caagaaccgg ttcgccgtgt tcgaccccac
     1981 gatgcacgcg gcgatcgtcg cccggcacgc gatgtcgacc gagctgtcgc acgcggtgac
     2041 cgagggcgag atcgacgtgt tctaccagcc ggtgctgtcg ctcgcgaccg ggctcacgtt
     2101 cggcgtcgag gcgctggccc gctggaagca tccgacccgc ggcttcgtcg agcccgacga
     2161 gttcatcccg ctggccgagg agagcggcac catcctcgcc ctcggccgcg cggtgctggt
     2221 cgaggcgtgt cgcgaggcgg cgccctggcg atcagggacc ggcgagggcg tctcgctcac
     2281 cgtcaacctc tcggcggccc agctggccca ggagacgttc atcgacgacc tcgtcgacat
     2341 cctgcgcgcc accggcttct cggcgtcgcg gctggtgctc gagatgaccg agaccgtcat
     2401 gttcagcgac acccagacga ccatcgcgcg cctgacggca cttcgggacc tgggcgtccg
     2461 gatcgcggtc gacgacttcg ggaccggcta ctcgtcgctc ggctacctcc gtcggttcaa
     2521 cgtcgacatc ctcaagatcg cgcgcgagtt catcgccccg cccgagtccg gac
//
LOCUS       DBNHXO010000108        11334 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_26609, whole genome shotgun sequence.
ACCESSION   DBNHXO010000108 DBNHXO010000000
VERSION     DBNHXO010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 11334)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11334)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11334
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_26609"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="AC162A_02210"
     CDS             <1..867
                     /locus_tag="AC162A_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016739880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIR5009680.1"
                     /translation="AAIMITNPSTLGLFERRIGELLDATHEAGALAYMDGANLNAILG
                     RFKPGEAGFDVMHFNTHKTFSTPHGGGGPGAGPVGVRAALAPFLPSPRVLAADDGSYR
                     LETWGERPTSIGRMRSFVGSTGVLVRAYAYLRAHGDAGLREVSDDAVLAANYLKARVG
                     VAYEIPFDRACKHEFVASAADLKHRTGVRTLDVAKRLIDKGYHPPTIYFPLTVEEAML
                     IEPTETESLETLDGFADALIEIAHEAEVDPELVTSAPHSAPVRRLDEATAARQPNLRW
                     RGMTGAQTPCPD"
     gene            1010..1579
                     /locus_tag="AC162A_02215"
     CDS             1010..1579
                     /locus_tag="AC162A_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5009681.1"
                     /translation="MAEDDVDALAVLFDRYRVPVYRAALALTGDPGASEEVLLDTFGR
                     AYTHRHRLRTDVSPLPWLHKVALNLCYSRLGRRRLRSEPVTEIVAERLVDGGVRPDTS
                     AEWSELCVIVREGIAGLSPKHQSVVILYYLEGRSLQETADALGLPLGTVKSRIHHALK
                     DLRGRLNADRRFGGAWGEASPDVAELGGS"
     gene            1576..2118
                     /locus_tag="AC162A_02220"
     CDS             1576..2118
                     /locus_tag="AC162A_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR5009682.1"
                     /translation="MTMPGSACRRHRNALIDFVDRRERTAGTPAALDHLARCERCEHE
                     MAEFALTIVALRRAGREIAAFEVPGASVARVVPPSPRSSGWSWRLQVGSLLTCAAIVG
                     LVILPSGPTSASYGDGTQSDARSTRLIPWRVAESRLARMPDVRSVSIVTLPQRYPDGL
                     TRPWKEVPASDATPREPTAS"
     gene            2346..4220
                     /locus_tag="AC162A_02225"
     CDS             2346..4220
                     /locus_tag="AC162A_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5009683.1"
                     /translation="MRKLVGLFASAAILVAACGGTSTTPAPASQPAATPPPASAPASG
                     EAPSASAAPAGEINLFGSAYAPAAPTAQGGSVIIGDWQEANEFNPFRSSQVTEANIGA
                     LTFHSLLTISDDFKYIPQLAAEPVPTTENGGVTVGEGGDAMTVTWKLRDGLKWSDGEA
                     LTCDDFKYAWEWTMDPDNVGVILAGFEDITAIDCPTDVDMVLHFKNVFEGYLTMFNSP
                     LPRHYLEKIPMKEQVAGAGFRPDEIANLPVSGPFKFASVTPLAETRLARNDNFVGAKS
                     GTAANLDEIVFKWYGDPDAMIAGYRGGEVDLAFDLADSDIPKVQDLGAEMHAIPALLY
                     EFLRPNWSPGPFTETGDHPNTGGCSRNPAVQDRGTGCPTADPAIRAAIAYAIDKNEIN
                     TRLLGGNAQVANTNISPSAWFYADQTPATFDPEKAKQILADGGWADSDGDGIVEKDGL
                     KAKIELCTTTRQVRQDTLALISAWLKNVGIDSVINPVDATAIFANYTEATNDIPCALS
                     RSNFDLAEHAFSSSIDPLGNYTSYHSSQFEPQGANDAQVSDPAADKALADVKSNVDFK
                     VVRDAMAVFQKAYVDNTIEIPLYYRKNVELANPRVGNYFANGTQAGSTWNGEDWFATN
                     "
     gene            4395..5513
                     /locus_tag="AC162A_02230"
     CDS             4395..5513
                     /locus_tag="AC162A_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009684.1"
                     /translation="MTKFVIRRALQAIPVLFGITIVVYGILLAAPGGPTAKFANNPKM
                     TLEQKEKFKAAWGLDQPIPIQYCRWMGFCNPLVPGTVLGSLPPPAAFIGPTGWPNLLP
                     TAISGAANGVLHGDFGYSISSGEKVSDRIARAALPTFILAGAALVVWLSIAIVLGVYS
                     AIHRYSLFDNSATVFSYVGFAMPTFWLGIMLIFIFAGTLHWLPASGMTDTRASPPFGS
                     DKYWEYFANNTLAAIGDISRHLLLPLITLVVVNIAGDSRFVRASMLESLGQDYVRTAK
                     AKGLPARVVNFKHALRNALLPVITNIGLEIPFLFTGAIVTETIFSWPGIGKLTIDATR
                     SFDYPILMGVLLVTATLVVFANLIADVAYGVVDPRIKY"
     gene            5523..6479
                     /locus_tag="AC162A_02235"
     CDS             5523..6479
                     /locus_tag="AC162A_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009685.1"
                     /translation="MAQNVEPLTTAAEIVDAPDPETAVDYEVGLKSLNQWQLAWRKFR
                     KHRLALIGLGLIGGLILVAVVGPILLPFAFTDIPKPDQIVSAGRPPSLQHPMGETGGL
                     QRDVLTLVVNGSRSSLLIGFSSMMIGVVLGTIVGAIAGFAGSMVDNVLMRIVDVMLSL
                     PILFVILVAAKFLGNGTVTMVIVIFGLFSWMGVSRLVRSQFLSIREREFVEAAHAVGV
                     RDRRIIFRHILPNAFSPIVVAASLIVAGNIIAEAFVSFLGFGVDPTSPTWGNILSGGL
                     QFITQGNWWWPLFPGLAIIVTVLAVNFVGDGLRDAFDPRSRA"
     gene            6476..7597
                     /locus_tag="AC162A_02240"
     CDS             6476..7597
                     /locus_tag="AC162A_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5009686.1"
                     /translation="MTEASTVRPVLGAPPSEKILLEVRDLRTSFKVMDGVVPAVDGTS
                     FSLPKGATLAIVGESGSGKSVTAMTIMRLLDIPPAEIGAGSEIWFDGREVLSLSDSEM
                     RKIRGNEMAMIFQEPLTSLNPVFTVGDQIGEQVKLHKKVSQREARDRSIEMLRLVGIP
                     TPERRVKQYPHELSGGMRQRVMIAMALSCEPKLLIADEPTTALDVTVQAQILELLKAI
                     QERTGAALLLITHDLGVVAEMVDNVIVMYAGQIVEQGTVHEVLLEPRMPYTMGLIESI
                     PTVEKRGGKLSAIKGVVPSPFHLPPACRFEPRCPYAWETCRTDPPELFPAGPSGRLAR
                     CNLHLGAARQRLDAAIANHDDNMTVDRRITGLLDTVEPA"
     gene            8140..8529
                     /locus_tag="AC162A_02245"
                     /pseudo
     CDS             8140..8529
                     /locus_tag="AC162A_02245"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
     gene            8731..9795
                     /locus_tag="AC162A_02250"
     CDS             8731..9795
                     /locus_tag="AC162A_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006375325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5009687.1"
                     /translation="MEGQPLLTVQGLKKHFPIYGGVLRRQIGTVYAVDGVDFTIMPGE
                     TFSLVGESGCGKTTLGRTVIQLTPSTGGRVVFDGYELADVDPDDMRPLRRRMQIIFQD
                     PFGSLNPRMPISDIIGEGLLAQGLTDRAARDKRVEDALELVGLRREYTRRYPHEFSGG
                     QRQRIGVARALALGPDFIVADEPVSALDVSIQSQVLNLLIDLKRDLHLTYLFISHNLS
                     VVQYFSDRVGVMYLGKIAEIGSVEQLYQDPRHPYTVALLSAIPEPDPRRRKSRLVLKG
                     DVPSPAAPPSGCRFHTRCWLRERLGNPENCSTIEPPLRPFGQEGHQVACHWAEDVNST
                     TVSQAVAEAPAIVVAAAEDE"
     gene            9792..10388
                     /locus_tag="AC162A_02255"
     CDS             9792..10388
                     /locus_tag="AC162A_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009688.1"
                     /translation="MNPAPRHDGHASEAPVGAAILLVVLLVAACGLASGVPTAGPVSP
                     GPAGSPPASADGELPSPTWWPGGVVEAVLLLGKSDLDVQAAGADLGASASTQDLEAMW
                     GAADGLTRVLVKLPSQVDRIRDYPTTVKLAAAYDAALPDMLAGTTKLRDSITAGDAAG
                     IAAGSQQMAKGLEAYSDVRTLLADAVGQAALMQRILVK"
     gene            complement(10402..>11334)
                     /locus_tag="AC162A_02260"
     CDS             complement(10402..>11334)
                     /locus_tag="AC162A_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5009689.1"
                     /translation="GCGGAAWYGFNAPAASAALAAADFDLKETYPLHVPDQPVPGLPD
                     PAGLASAVQAQLEETVGVHTEIDTMPVEDYFADLATGGLDGLYLGGVGSTIADPEAFL
                     EPLFRKGLKSTPATRTPKAAAALADAASTADPAERADAFARANDAIRDAAPLIPLVHP
                     GSVTAFRSDVTDVVTSPLGLDPLGSFTPGDRGQLVFMQATEPDGAYCADQPSADAYRL
                     CGLVVEGLYGFAPGTLAVEPRLAQRCEPDAEAVVWTCRLRQGATFSDGSRVDAGDVLA
                     SYVAQWDTSQPLRADSQASFTAWTELFGDTIGGE"
BASE COUNT         1706 a         4098 c         3799 g         1731 t
ORIGIN      
        1 gccgcgatca tgatcaccaa cccctcgacg ctcggcctgt tcgagcgacg gatcggcgag
       61 ctgctcgacg cgacccacga agccggcgcg ctcgcctaca tggacggcgc caacctcaac
      121 gcgatcctcg ggcggttcaa gcccggcgag gccggcttcg acgtgatgca cttcaacacc
      181 cacaagacgt tcagcacgcc ccacggcggc ggcgggcccg gcgccggccc cgtcggggtt
      241 cgggccgcgc tcgcgccgtt cctgccctcg ccgcgggtcc tcgccgcgga cgacggctcg
      301 tatcgcctgg agacctgggg ggagcgcccg acctccatcg gccggatgcg ctcgttcgtg
      361 ggcagcaccg gcgttctcgt gcgcgcctat gcgtacctgc gcgcgcacgg cgacgcgggc
      421 ctgcgcgagg tctcggacga tgccgtgctg gccgcgaact acctcaaggc ccgcgtcggc
      481 gtcgcgtacg agatcccgtt cgatcgcgcc tgcaagcacg agttcgtcgc ctccgcggcc
      541 gatctcaagc accgcaccgg cgtgcggacc ctcgacgtgg cgaagcgttt gatcgacaag
      601 ggctaccatc cgccgacgat ctacttcccg ctgaccgtcg aggaggccat gctgatcgag
      661 cccacggaga ccgaatcgct cgagacgctc gacggattcg cggatgcgct gatcgagatc
      721 gcgcacgagg cggaggtcga tcccgagctc gtcacctcgg ccccgcacag cgcgccggtc
      781 cgacgcctcg acgaggcgac cgccgctcgc cagccgaacc tccgctggcg cggcatgacc
      841 ggcgcccaga cgccctgtcc ggactgatcc gaaccggaaa tgaacctcct gctccctcag
      901 cccgtatggt tggctacgag ggagcgctga cgctcacgga gaggcaccaa tcgaccgcga
      961 gacccgcgcc gacaaacccg cgccccccga cgccgtgctc attcgccgta tggcggagga
     1021 cgacgtcgat gcgctcgccg tcctgttcga ccgctaccgg gtgccggtgt accgtgccgc
     1081 gctcgcgctg acgggtgacc ccggcgcctc ggaggaggtg ctcctggaca cgttcgggcg
     1141 tgcctacacg caccgccacc gcctgcggac cgacgtctcg ccgcttccct ggctgcacaa
     1201 ggtcgcgctg aacctgtgct acagccggct cggccgtcgg cggctgcgca gcgagccggt
     1261 gacggagatc gtcgccgagc gcctcgtgga cggcggcgtc cgccccgaca ccagcgccga
     1321 gtggtccgag ctgtgcgtga tcgtgcgcga ggggatcgcg ggcctgtcgc ccaagcacca
     1381 gtcggtcgtg atcctgtact acctcgaggg acgctcgctc caggagaccg ccgacgcgct
     1441 ggggctgccg ctggggaccg tcaagagccg gatccaccac gcgctgaagg acttgcgcgg
     1501 tcgcctcaac gcagatcgcc ggttcggcgg agcgtggggc gaggcgtcgc ccgacgtggc
     1561 ggagctgggc gggtcgtgac gatgcccgga tcggcctgcc gccgccaccg caacgcgctg
     1621 atcgacttcg tcgaccgccg cgaacggacg gccggaacgc ccgcggccct cgaccacctg
     1681 gcgcgctgcg agcggtgcga gcacgagatg gcggagttcg ccctgacgat cgtcgcgctc
     1741 cggcgagcgg gtcgcgagat cgcggcgttc gaggtgcccg gcgcgtccgt ggcgcgggtc
     1801 gtgccgccaa gcccgcgttc cagcggctgg tcgtggcgat tgcaggtcgg cagtctgctc
     1861 acctgcgcgg cgatcgtcgg gctcgtgatc ctgcccagcg ggccgacgtc ggcctcctat
     1921 ggcgacggaa cccagtcgga cgctcgttcg acgcgattga tcccctggcg cgtcgccgag
     1981 tcgcgccttg cacggatgcc ggacgtgcgc tccgtgtcca tcgtcaccct gccccaacgc
     2041 taccccgacg gcctgacccg gccgtggaag gaggtgccag cgtccgacgc gacgccgcgc
     2101 gagccgactg cctcctgagg cgccgggacc tttcggcccg tcgccatccg gggtgcattt
     2161 gacactcgac ctgcggtaag cgatcctgcg ccccagcgca gccccgttgg ccgctgtgtg
     2221 tgcgcgtgtg cgcacgattt ccccatcacc ccaagggccg tgtgtgccga tgggcgggga
     2281 cagtggcccg gtggactgtc agggagtcat tgttcaggac ccctacgcaa cttggaggag
     2341 ccttgatgcg gaaattggtg ggcctgttcg caagcgcggc catcttggtc gcagcgtgcg
     2401 gcggcaccag caccacccct gccccggcga gccagccggc ggcaacgccc cccccggcga
     2461 gcgcaccggc atccggtgaa gccccgtcgg catcggctgc gccggccggc gagatcaacc
     2521 tgttcggcag cgcctatgcg cccgccgcgc cgaccgccca gggcggctcg gtcatcatcg
     2581 gcgactggca ggaagccaac gagttcaacc cgttccggtc gtcacaggtc accgaggcca
     2641 acatcggtgc cctcaccttc cacagcctgc tgacgatcag cgacgacttc aagtacatcc
     2701 cgcagctggc cgcggagccg gtcccgacca ccgagaacgg tggcgtcacg gtcggtgagg
     2761 gcggcgacgc gatgaccgtc acctggaagc ttcgcgacgg gctcaagtgg tccgacggcg
     2821 aggccctcac gtgcgacgac ttcaagtacg cgtgggagtg gaccatggac cccgacaacg
     2881 tcggcgtgat cctggccggc ttcgaggaca tcaccgcgat cgactgcccg acggacgtcg
     2941 acatggtcct gcacttcaag aacgtcttcg agggctacct cacgatgttc aactcgccac
     3001 tgccgcgcca ctatctggaa aagatcccga tgaaggagca ggtcgccggc gccggcttcc
     3061 ggcccgacga gatcgccaac ctcccggttt ccggaccgtt caagttcgcg tcggtgacgc
     3121 ccctggccga gacccggctg gcgcgcaacg acaacttcgt gggcgccaag tccggcacag
     3181 ccgccaacct cgacgagatc gtcttcaagt ggtatggcga ccctgacgcg atgatcgccg
     3241 gctaccgcgg cggcgaggtc gacctcgcgt tcgacctcgc ggactcggac atcccgaagg
     3301 tccaggacct gggcgcggag atgcacgcca tcccggcgct gctgtacgag ttcctgcgcc
     3361 ccaactggtc gcccggcccg ttcaccgaga ccggcgacca cccgaacacc ggtggctgct
     3421 cgcgcaaccc ggcggtccag gaccgcggca cgggctgccc gaccgccgac ccggcgatcc
     3481 gcgcggcgat cgcgtacgcg atcgacaaga acgagatcaa cacgcgcctc ctcggcggca
     3541 acgcccaggt cgcgaacacg aacatcagcc cgtcggcctg gttctacgcg gaccagaccc
     3601 cggcgacgtt cgatccggag aaagccaagc agatcctcgc cgacggcggc tgggccgatt
     3661 ccgacggcga cggcatcgtc gagaaggacg gcctcaaggc caagatcgag ctgtgcacga
     3721 cgacgcggca ggtccgccag gacacgctcg cgctcatcag cgcctggctc aagaacgtcg
     3781 gcatcgacag cgtgatcaac cccgttgacg cgactgcgat cttcgccaac tacaccgagg
     3841 cgaccaacga catcccgtgc gccctctcgc ggagcaactt cgacctcgcg gagcacgcgt
     3901 tcagctcgtc gatcgatccc ctcggcaact acaccagcta ccacagcagc cagttcgagc
     3961 cccagggcgc gaacgatgct caggtgagtg acccggccgc cgacaaggcg ctcgccgacg
     4021 tcaagtccaa cgtcgacttc aaggtggtgc gcgacgcgat ggcggtcttc cagaaggcct
     4081 acgtcgacaa caccatcgag atcccgctct actaccgcaa gaacgtcgag ctcgcgaacc
     4141 cgcgggtcgg caactacttc gccaacggca cccaggccgg ctcgacctgg aacggcgagg
     4201 actggttcgc cacgaactag gccgaggctt ctcgcgaagc acacgaacgg ggcgtccggt
     4261 atccggacgc cccgtttcga ctaccagcgg gcgtgacccc gataggtgta ctgtccgcgc
     4321 aagccccgtt cgctcgggat ttcccggtcg gacggtccag ccaaccgcat gctccgggtt
     4381 gaggccgaac gccgatgacc aagttcgtga tccgccgcgc gctgcaggcg attccggtgc
     4441 tgttcgggat caccatcgtc gtctacggga tcctgctcgc cgcgcccggc gggccgaccg
     4501 cgaagttcgc caacaacccc aagatgaccc tcgagcagaa agagaagttc aaggccgctt
     4561 ggggcctcga tcagccgatt ccgatccagt actgccgctg gatggggttc tgcaatccgc
     4621 tcgtccccgg gacggtcctc gggtccctgc cgccgcccgc cgcgttcatc gggccgacgg
     4681 gctggccgaa cctgctgccc accgcgatca gcggcgccgc caacggcgtg ctgcacggtg
     4741 acttcgggta ctcgatctcg tcgggcgaga aggtcagcga ccggatcgcc cgggctgccc
     4801 tgccgacctt catcctcgcg ggcgcggcgc tggtcgtgtg gctgtcgatc gcgatcgtgc
     4861 tcggcgtgta ctcggcgatc caccgctact cgctattcga caactcggcg accgtgttct
     4921 cctacgtcgg ctttgccatg cccacgttct ggctgggcat catgctgatc ttcatcttcg
     4981 ccggcaccct tcactggctt ccggccagcg gcatgacgga cacgcgggcc tcgccgccgt
     5041 tcggcagcga caagtactgg gagtacttcg cgaacaacac gctcgcggcg atcggtgaca
     5101 tcagccgtca cctcctgctg ccgctcatca cgctcgtcgt cgtcaacatc gccggggaca
     5161 gccggttcgt ccgggcgagc atgctcgagt cgctgggcca ggactacgtg cgtactgcca
     5221 aggcgaaggg cctgcccgcg cgcgtcgtga acttcaagca cgcgctgcgc aatgcgctgc
     5281 tgccggtgat caccaacatc ggcctcgaga tcccgttcct gttcacgggt gcgatcgtga
     5341 ccgagacgat cttctcgtgg ccggggatcg ggaagctcac catcgacgcc acacggagct
     5401 tcgactaccc gatcctgatg ggcgtcctgc tggtcacggc cacgctggtc gtgttcgcga
     5461 acctcatcgc agacgtcgcg tacggcgtcg tcgatccccg catcaagtac tgaggtccga
     5521 cgatggccca gaacgtcgag ccgctgacca ctgcggcgga gatcgttgat gcccccgacc
     5581 cggagaccgc ggtcgactac gaggtcgggc tcaagtcgct caaccagtgg cagctcgcgt
     5641 ggcgcaagtt ccgcaagcat cgcctcgcgc tcatcggcct cgggctgatc ggcggcctga
     5701 tcctggtggc cgtcgtcggc ccgatcctgc tgccgttcgc gttcacggac atcccgaagc
     5761 ctgaccagat cgtgtcggcg ggccgcccac cctcgctcca gcatccgatg ggcgagaccg
     5821 gtggcctgca gcgggacgtg ctgacgctcg tggtcaacgg gtcccggtcg tcgctgctca
     5881 tcggcttctc gagcatgatg atcggcgtgg tcctgggcac gatcgtggga gcgatcgccg
     5941 ggttcgccgg cagcatggtc gacaacgtcc tgatgcggat cgtcgacgtg atgctgagcc
     6001 tgccgatcct gttcgtgatc ctcgtcgccg ccaagttcct cggcaacggg acggtgacca
     6061 tggtgatcgt gatcttcgga ctgttctcgt ggatgggcgt gagccgcctg gtgcgcagcc
     6121 agttcctgtc catccgcgag cgggagttcg tcgaggccgc gcacgccgtc ggcgtgcggg
     6181 accggcggat catcttccgc cacatcctgc ccaacgcgtt cagcccgatc gtcgtcgcgg
     6241 catcgctgat cgtggcgggc aacatcattg ccgaggcgtt cgtgagcttc ctcgggttcg
     6301 gcgtcgaccc gacgtcaccc acgtggggca acatcctctc cggcgggctg cagttcatca
     6361 cccagggcaa ctggtggtgg ccgctgttcc cgggtctcgc gatcatcgtg acggtgctgg
     6421 ccgtgaactt cgtgggagac ggcctgcggg acgcgttcga tccgagatcg cgagcatgac
     6481 cgaggcctcg accgttcgcc ccgtcctcgg ggcaccgccg tccgagaaga tcctgctcga
     6541 ggtccgcgac ctgcggacct cgttcaaggt gatggacggc gtggtgcccg ccgtcgacgg
     6601 gaccagcttc tcgctgccga agggggcgac gctggccatc gtcggcgaat cggggtcggg
     6661 caagagcgtg accgcgatga cgatcatgcg cctgctggac atcccaccgg ccgagatcgg
     6721 tgcgggcagt gagatctggt tcgacggtcg cgaggtcctg tcgctgtccg acagcgagat
     6781 gcgcaagatc cgcggcaacg agatggcgat gatcttccag gagccgctca ccagcctcaa
     6841 tcccgtgttc acggtcggcg accagatcgg cgagcaggtg aagctccaca agaaggtcag
     6901 ccagcgcgag gcgcgcgacc gctcgatcga gatgctccgg ctggtcggca ttccaacccc
     6961 ggaacggcgg gtgaagcagt atccccacga gctgtcgggc ggcatgcgcc agcgcgtgat
     7021 gatcgcgatg gcgctctcgt gcgagcccaa gctcctgatc gccgacgagc cgacgacggc
     7081 gctcgacgtg accgtccagg cgcagatcct ggagctgctc aaggcgatcc aggagcggac
     7141 gggcgcggcg ctgctgctga tcacgcacga tctcggggtg gtcgccgaga tggtcgacaa
     7201 cgtgatcgtg atgtacgccg gccagatcgt cgagcagggg accgtgcacg aggtgctgct
     7261 cgagccgcgg atgccctaca cgatgggcct catcgagtcg atcccgacgg tggagaagcg
     7321 gggcggcaag ctgtccgcga tcaagggcgt cgtcccctcg ccgttccacc tgccgcccgc
     7381 gtgccggttc gagccgcgtt gtccatatgc ctgggagacg tgtcggaccg acccgcccga
     7441 gctctttccg gccgggccca gcggccgcct cgcccgctgc aacctccacc tcggagcggc
     7501 gcgccagcga ctggatgcgg cgatcgccaa tcacgatgac aacatgacgg tcgaccgccg
     7561 gatcaccggc ctgctggaca cggtcgagcc cgcatgacca ccaccatcga ggccgcgccc
     7621 caggccgagc cgatcatccg gcgcaagctt cgccgccgcc cgccgaccgc gatccgcaac
     7681 gggcgtgccc tgttgctctc ggtcgcgggc tacgcgctca ccggcctgtt cgtcgtgctg
     7741 ctcggcgtcg tgatcggcgc caagggcgcc ttcggcatcc tgctgccggc catcctggtc
     7801 agcgtccccc tcacgctcgt cgtgtacggc gtcgttcgcc tcacccgaaa ccgccagaag
     7861 tggtcgctcg cgctcgcgct cgggctcgac atcctcctgt cactcgcgtg cggctacctg
     7921 atcgtgacca tgcagacgcg gaccagcgac ccgaacgcga ccttcttcct ccagatgctg
     7981 ctcaccccgc cgctggtctt cgccatcgcc tcggcgtggc acttcgtgcg cggggcgagc
     8041 tggttcaacg gcgtctacga acgtccgctc ctgcccgagg aggccggcgc gcggccgatc
     8101 acgtccccgc tggtggccgg tggcccgacg acgcccgtca tgaccgggcc cgcgctcgcc
     8161 gagtacgcgc cccgccttgg cgcctatctc gccgacctcg tcatcgtggc catcctgacc
     8221 gcggtgccgg tcgtcgccgg gctcgcgatc agctccgccc tgggcggcgg catcgggcaa
     8281 ttgatcgcct acgcgggcgt gatcctcggc atcctcgtca acctgggcta cttcgcctat
     8341 ttctggagcc acggcggcac gacgccgggc atgcggcccg tgcatctcaa ggtggtgcac
     8401 gagatgacgc ccgatccgct tacctggggc aacgctatcc ggcgctacat cgtgttcgtg
     8461 ttcagcgcgg ccgccctgta catcggcttc gcctgggtat tcatcgaccc gcggcgcaag
     8521 gggtggcacg acatcgccgc acggacgatc gtgatcgacg cgcgcgccca ggccgccgag
     8581 gaggccaagg tcgccgacgc cgcggccgcg gcggtcgccg ccggcacggc cacccccgac
     8641 gaggtcgcgg atgtcgaccc gatgtcggag gccgacgccc cgatcatcga ggctgtcgtg
     8701 gccccggagg cggcgaggcc ggtctcggtc atggagggcc agccgctgct gaccgtgcag
     8761 gggctcaaga agcacttccc gatctacggc ggcgtcctgc gccgccagat cggcacggtc
     8821 tatgcggtcg acggcgtcga cttcaccatc atgcccggcg agaccttcag cctcgtcggc
     8881 gagtccggct gcggcaagac gacccttggc cggaccgtca tccagctgac gccctcgacc
     8941 ggcggccgcg tcgtcttcga cggctacgag ctggccgacg tcgaccccga cgacatgcgg
     9001 cccctgcgcc ggcggatgca gatcatcttc caggacccgt tcgggtcgct caacccgcgg
     9061 atgccgatct cggacatcat cggggagggg ctcctcgccc aggggctgac ggaccgcgcc
     9121 gcccgcgaca agcgcgtcga ggatgccctc gagctggtcg gcctgcggcg cgagtacacg
     9181 cggcgctacc cgcacgagtt ctccggcgga cagcggcagc gcatcggggt cgcccgggcg
     9241 ctcgcgctgg gccccgactt catcgtcgcc gacgagcccg tgtccgcgct cgacgtctcg
     9301 atccagagcc aggtcctcaa cctgctgatc gatctcaagc gcgatctgca cctcacctac
     9361 ctgttcatca gccacaacct gtcggtggtc cagtacttct ccgaccgcgt cggcgtgatg
     9421 tacctcggca agatcgccga gatcgggtcg gtcgagcagc tgtaccagga tccccgccac
     9481 ccgtacacgg tcgcgctgct gtcggcgatc cccgagcccg atccgcgccg ccgcaagtcg
     9541 agactcgtgc tcaaggggga tgtgccgtcg cccgcggccc cgccctcggg ctgccggttc
     9601 cacactcgct gctggctccg ggagcggctg ggcaatcccg agaactgctc gaccatcgag
     9661 ccgccgctgc ggccgttcgg gcaggaaggg caccaggtcg cctgccactg ggccgaggac
     9721 gtcaactcga ccacggtgtc ccaggccgtt gccgaggctc cggcgatcgt cgtggcggcc
     9781 gccgaggacg aatgaacccg gccccgcgtc acgacggcca cgccagcgag gcgccggtcg
     9841 gagctgccat tctgctcgtg gtcctgctcg tcgcggcgtg cgggctggcc tccggcgtcc
     9901 cgacggccgg gcctgtgtcg cccggcccag ccggttcgcc gccggcgtcc gccgatggcg
     9961 agctgcccag cccgacgtgg tggcccggcg gcgtcgtcga ggccgtgctc ctgctcggca
    10021 agtcggacct cgacgtccag gccgccgggg cggatctcgg cgcgtcggcc tcgactcagg
    10081 acctcgaagc gatgtggggc gcggccgacg gcctgaccag ggtgctcgtg aagctgccgt
    10141 cccaggtcga tcggatccgg gactacccga ccacggtcaa gctggcggcg gcctacgacg
    10201 ccgcgctgcc cgacatgctc gccgggacca cgaagctgcg cgactcgatc acggcgggcg
    10261 acgccgccgg catcgcggcg ggcagccagc agatggccaa ggggctcgag gcgtacagcg
    10321 acgtccggac gctgctcgcc gatgccgtgg ggcaggccgc gctgatgcag cggatcctcg
    10381 tcaagtagga ccgggccgcc actactcgcc cccgatcgtg tccccgaaca gctcggtcca
    10441 ggcggtgaat gaagcctgcg agtcggctcg gagcggctgc gaggtgtccc actgggcgac
    10501 gtacgaggcg agcacgtcgc cggcgtcgac gcgactcccg tcgctgaacg tcgcgccctg
    10561 ccgcagccgg caagtccaga ccacggcctc cgcgtcgggc tcgcagcgct gcgcgaggcg
    10621 cggctcgacc gccagcgtcc ccggtgcgaa gccgtacagg ccctccacga cgagcccgca
    10681 caggcgatag gcatcggccg acggctggtc cgcgcagtag gcgccgtcgg gctccgtcgc
    10741 ctgcatgaac acgagctggc cgcggtcgcc gggggtgaac gaacccagtg gatcgaggcc
    10801 cagcggcgag gtcaccacgt cggtcacatc ggagcggaag gccgtgaccg agcccgggtg
    10861 gaccagcggg atcagcggcg cggcgtcgcg gatcgcgtcg ttggcgcgcg cgaacgcgtc
    10921 cgcgcgctcc gccgggtcgg cggtggaggc ggcgtccgcg agggccgccg cggccttcgg
    10981 cgtgcgcgtc gcgggcgtgg acttgagtcc cttgcggaag agcggctcca ggaacgcctc
    11041 gggatccgcg atcgtcgagc ccaccccgcc gaggtacagg ccgtcgagcc cgcccgtcgc
    11101 caggtcggca aagtagtcct cgacgggcat cgtgtcgatc tccgtgtgca cgcccacggt
    11161 ctcctccagc tgcgcctgga ccgccgaagc aagccctgcc gggtcgggga gtcccggaac
    11221 cggctggtcc ggaacgtgga gcgggtacgt ctccttgagg tcgaagtcgg cggcggccag
    11281 cgccgcggag gcggccgggg cgttgaaccc gtaccacgcg gcgcccccgc atcc
//
LOCUS       DBNHXO010000109         4743 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5384993, whole genome shotgun sequence.
ACCESSION   DBNHXO010000109 DBNHXO010000000
VERSION     DBNHXO010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5384993"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="AC162A_02265"
     CDS             <1..453
                     /locus_tag="AC162A_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009690.1"
                     /translation="PASHEEPAVTQSSFAAALPPGPLVAAGEYRPGVCNIGPEEIARR
                     RRAGHVGLVATVALFVALVVVGAPPIARLLVALPATIAASGYLQAWLRFCAGFGSRGV
                     FNFGEVGPTEHVGDADARRRDRARARQIGGAALLIGVATGIIAVLLPV"
     gene            612..2051
                     /locus_tag="AC162A_02270"
     CDS             612..2051
                     /locus_tag="AC162A_02270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5009691.1"
                     /translation="MPDLAAAPAATAAQANPATFVTDSESAVSRSDLRALRAAIDGRV
                     LVAGDLGFDAARGVHNSHYDRQPAVIVRPASAVDVSRAVTTARELDLEIAVKGGGHSV
                     AGHSATDGGMLIDLGAMRDIGIDPIRRIGSAQGGATAGEYTAAAGARGFATPFGDTGS
                     VGLGGLTLGGGIGWLARKHGLTIDSLIEVDLVTADGRLVTVSEAADPDLFWAIRGGGG
                     NFGIATRFRYRLHPIEMVTGGMLVLPLTHQVLRDLVDVSVAAPEELTQISFVMGLPPA
                     PFVPPEAVGTPAVLVMPVHAGPLDEGTAAMAPFRAIATPLADMVGPMPYPAMYQLTAA
                     GEQPGASVIRSAFMPDLDSAAIDTIVERHRSPEGALGMTQLRVLGGEMARVPAGATAF
                     AHRDATVMATVLAHGEAPYEDLKAYAESYFADLSGQSTGVYSNFLGEEAPNRLHAAYP
                     GATYERLVQVKRRVDPDNVFHANHNIRPD"
     gene            2234..3616
                     /locus_tag="AC162A_02275"
     CDS             2234..3616
                     /locus_tag="AC162A_02275"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR5009692.1"
                     /translation="MQPAQTPIADPAAPPGETFAAEPPVVEPVVPLPPPPLAGGQPRK
                     SGHSRWVIGVLAPVLAVVVFAGGVAVGNSGLAGGSTANGSPTGSAAGAADLALIEEAW
                     RAIQDNYVDAKHLNDRDLAYGAIRGLTEAVGDEGHTSFLTADEAKAVDQSLSGTFVGI
                     GVQLDEDETKGPVIRSVIPGTPAEEAGLRRGDVITQVDGTTTDGEEIDEVVSRVRGPE
                     GEPVTLTIKRGEAVPFDVTITRKKFDLPLVSWAMVPGRKIAMIRLDQFATGATKGIEE
                     AIKGAKAADATAIVFDLRGNPGGYVNEAVGVASQFVGDGTVYRSVDASGTEKDIAVEP
                     GGLATGLPLVVLTDGNTASSAEIVTGAIQDADRGTVVGDKTFGTGTVLGRFDLADGSS
                     LRIGVERWLTRDGRPIWHEGLEPDVKVSLADTVVPLLPDDIRDLTPAKLAESDDTQLL
                     KALEILKGEG"
     gene            complement(3672..3878)
                     /locus_tag="AC162A_02280"
     CDS             complement(3672..3878)
                     /locus_tag="AC162A_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009693.1"
                     /translation="MTDARKASPRLPPRRPTPGPDPRKHLIAEYKHRLQEVWCICGWT
                     GSSASVNGRTSDWSRHVAASRETT"
     gene            complement(3883..4260)
                     /locus_tag="AC162A_02285"
     CDS             complement(3883..4260)
                     /locus_tag="AC162A_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HIR5009694.1"
                     /translation="MNVDKTLTCADCGQAFTFTASEQDFFAERGFTEPRRCATCRANR
                     KAARNAEGGGGGYSSGAGYSSGGGYSSGGYSSGGGGGYGSRGPREMFPATCSSCGQET
                     EVPFKPTTGKPVYCSACFAQRRA"
     gene            complement(4432..>4743)
                     /locus_tag="AC162A_02290"
     CDS             complement(4432..>4743)
                     /locus_tag="AC162A_02290"
                     /inference="COORDINATES: protein motif:HMM:NF016854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HIR5009695.1"
                     /translation="ERFDAAAARHPAAVRRKMFGYPALFVGGNLATGLFADTWMVRLA
                     PADLEVLVALPGAGPFAPMPGRTMKGYATLPPDVVAQDAELDAWLERAITFAGSLPAK
                     K"
BASE COUNT          659 a         1740 c         1711 g          633 t
ORIGIN      
        1 cccgcgtccc acgaggagcc agccgtgacc cagtcgagct tcgccgccgc cctcccgccc
       61 ggcccgctcg tcgccgctgg cgagtaccgg cccggcgtct gcaacatcgg ccccgaggag
      121 atcgcgcggc ggcgccgtgc cgggcacgtc ggcctggtgg cgaccgtcgc gctgttcgtc
      181 gccctggtcg tcgtcggcgc accaccgatc gcccggctcc tcgtcgcgct gccggcgacc
      241 atcgcggcat ccggctacct gcaggcgtgg ctcaggttct gcgccgggtt cggctcgcgc
      301 ggcgtgttca acttcggcga ggtggggccg acggagcacg tcggtgacgc ggacgcgcgg
      361 cggcgcgacc gggcgcgagc acgccagatc ggcggcgctg cgctgctgat cggcgtcgca
      421 acggggatca tcgcggtcct gctgccggtc tgacccctgc ctcatcgtca ttcgtccagc
      481 acgggcgcgg accgccgtcg ggccgcgctg gcatcgcacg tccggtggcg ccggcgaggc
      541 gcccggcgag gatcaggtca ccgatccacc gatcgccctg aacctatcga ccgtccaagg
      601 agacgaccgc gatgccggac ctcgccgccg cccccgccgc taccgcagcg caggccaacc
      661 ccgccacctt cgtcacggac tccgagtccg cggtctcacg ctccgacttg cgcgcgcttc
      721 gtgcggccat cgacggccgg gtcctcgtcg ccggcgacct cggctttgac gcggcccgcg
      781 gtgtccacaa cagccactac gaccggcagc ccgccgtgat cgtccgtccc gcgagcgcgg
      841 tcgacgtttc ccgcgccgtg acgaccgccc gcgagctgga cctcgagatc gcggtcaagg
      901 gcggcggcca cagcgtggcc gggcactcgg caaccgacgg cgggatgctg atcgacctgg
      961 gcgcgatgcg cgacatcggc atcgacccga tccgccgcat cgggtccgcc cagggcggcg
     1021 cgaccgccgg cgagtacacc gcggcagccg gcgcgcgggg cttcgcgacc ccgttcggcg
     1081 acaccgggtc cgttggcctc ggcggcctga cgctcggcgg cggcatcggc tggctcgccc
     1141 gcaagcacgg cctgacgatc gacagcctga tcgaggtgga cctcgtcacg gcggatggcc
     1201 ggctggtcac cgtcagcgag gcggccgatc ccgacctgtt ctgggcgatc cggggcggcg
     1261 gcggcaactt cggcatcgcg acccggttcc gctaccggct gcacccgatc gagatggtca
     1321 ccggcggcat gctcgtcctg ccgctgaccc accaggtgct gcgtgacctc gtcgacgtgt
     1381 ccgtggccgc gccggaggag ctgacccaga tctcgttcgt gatgggcctg ccgcccgcgc
     1441 cgttcgtgcc gcccgaagcc gtcgggacgc ccgccgtcct cgtgatgccc gtccatgcgg
     1501 gtccgctcga cgaggggacc gccgcgatgg cgccgttccg cgcgatcgcc acgccccttg
     1561 ccgacatggt cggccccatg ccctacccgg cgatgtacca gctgaccgcg gccggcgagc
     1621 agccgggggc gagcgtcatc cgctcggcgt tcatgcccga cctcgacagc gccgcgatcg
     1681 acacgatcgt cgagcgtcac cggagcccgg agggtgccct cgggatgacc cagctccgcg
     1741 tcctcggcgg cgagatggcg cgcgtgcccg ccggtgcgac cgcgttcgcg catcgcgacg
     1801 cgacggtgat ggccacggtg ctcgcccacg gcgaggcccc gtacgaggac ctcaaggcgt
     1861 acgcggagtc gtacttcgcc gacctgtccg gccagtcgac cggcgtgtac tcgaacttcc
     1921 tcggcgagga ggcgccgaac cgcctgcacg cggcctatcc gggcgcgacg tacgagcggc
     1981 tcgtccaggt caagcggcgc gtcgatcccg acaacgtgtt ccacgccaat cacaacatcc
     2041 ggcccgactg accgctcccg cgcgtccgcg cccccttcaa cgcccccgac ggaccccgtc
     2101 gggggcgcat ccgtgtcacg gagacgtaag gcaacttcac ccgtccgcta ccgtggctga
     2161 agggtcaggc atcatgctgc ccggagtgcc cgcgtcctcg cgcgcctgaa gcccagagat
     2221 gatgagcgac cccatgcagc ccgcccagac ccccatcgcc gacccggcgg cacctcccgg
     2281 cgagaccttc gccgccgaac ccccggtcgt cgagccggtc gtgccgctgc ccccgccgcc
     2341 cctggcgggc ggccagcccc gcaagagcgg gcactcgcgc tgggtgatcg gcgtgctggc
     2401 gcccgtcctc gcggtcgtcg tgttcgccgg tggcgtcgcc gtcggcaaca gcgggctcgc
     2461 gggcggatcc accgcgaacg ggtcgccgac cgggtcggcc gccggcgccg ccgacctcgc
     2521 gctgatcgaa gaggcgtggc gggcgatcca ggacaactac gtcgacgcca agcacctcaa
     2581 cgaccgcgac ctcgcctacg gcgcgatccg cggcctgacc gaggccgtcg gcgacgaggg
     2641 tcacacgtcg ttcctgaccg ccgacgaggc gaaggccgtc gaccagtcgc tttcggggac
     2701 gttcgtcggc atcggcgtcc agctcgacga ggacgagacc aagggcccgg tcatccggtc
     2761 cgtgatcccg ggcacgcccg ccgaggaggc ggggctgcgg cgcggcgacg tgatcacgca
     2821 ggtcgacggc accacgacag acggtgagga gatcgacgag gtcgtctcgc gggtccgcgg
     2881 tcccgaaggc gagccggtca cgctcacgat caagcgcgga gaggccgtcc cgttcgacgt
     2941 gacgatcacc cgcaagaagt tcgacctgcc gctcgtcagc tgggcgatgg ttcccggccg
     3001 caagatcgcc atgatccgcc tcgaccagtt cgcgaccggc gcgaccaagg gcatcgagga
     3061 ggcgatcaag ggggccaagg ccgcggatgc gacggcgatc gtgttcgacc tgcggggcaa
     3121 tccgggcggc tacgtcaacg aggccgtggg cgtcgcgagc cagttcgtcg gcgacggcac
     3181 cgtctaccgc agcgtcgacg cgagcgggac ggagaaggac atcgcggtcg agcccggggg
     3241 ccttgccacg ggcctgccgc tggtggtgct cacggacggg aacaccgcga gctcggcgga
     3301 gatcgtcacg ggcgccatcc aggacgccga ccgcgggacg gtggtggggg acaagacgtt
     3361 cgggacgggg accgtgctcg ggcgcttcga cctggcggac gggtcgtcgc tgcggatcgg
     3421 cgtcgagcgc tggctgacac gtgacggccg cccgatctgg cacgagggcc tcgagccgga
     3481 tgtgaaggtg tcgctggccg acacggttgt gccgctgctg cccgacgaca tccgggacct
     3541 gaccccggcc aagctcgcgg agagcgacga cacgcagctg ctgaaggccc tggagatcct
     3601 caagggcgag ggctgacgca aagaagggcg cccggcaggg cgcccttcga gacggtcaga
     3661 ggcgggtcag gtcaggtcgt ctcgcgtgag gccgccacgt ggcggctcca gtcggacgtc
     3721 cggccgttca ccgacgccga gctcccggtc cagccgcaga tgcaccagac ctcctggagg
     3781 cggtgcttgt actcggcgat gaggtgcttg cgggggtccg ggccgggcgt cggtcgccgc
     3841 ggtggcagac gcggcgatgc cttgcgggcg tcggtcaccc gatcaggccc ggcgctgcgc
     3901 gaagcaggcg ctgcagtaga ccggcttgcc ggtcgtgggc ttgaagggga cctcggtctc
     3961 ctggccgcac gacgagcagg tggccgggaa catctcccgc ggccccctcg agccgtagcc
     4021 gccaccgccg cccgacgagt agccgccgga cgagtagccg ccgcccgacg agtagccggc
     4081 gccggaggaa tagccgccac cgccgccctc ggcgttgcgg gcggccttgc ggttggcccg
     4141 acaggtggcg caccggcggg gctcggtgaa cccgcgctcg gcaaagaagt cctgctcgct
     4201 ggcagtgaag gtgaaggcct ggccacagtc ggcgcaggtg agcgtcttgt cgacgttcat
     4261 gtcgatccac tcctggaact tgcgacggtc tcgccgatcg gcctcggtgt cagccggggt
     4321 ggtggatcga gtgtgccgta ctggtatcgc ccgacgatgg ggcgtcgacg gaggatacgc
     4381 gatcctgcgc catacggcga ccccccagat gcacgggggt cccgataggc attacttctt
     4441 ggcgggcagg ctcccggcga acgtgatcgc gcgctcgagc caggcgtcca gctcggcgtc
     4501 ctgggcgacc acgtccgggg gcagcgtcgc gtagcccttc atcgttcggc ccggcatcgg
     4561 cgcgaagggc cccgcgccgg gcagcgcgac caggacctcg aggtcggccg gcgccaaccg
     4621 gaccatccag gtgtcggcga acaggccggt cgcgaggttg ccgccgacga acagggccgg
     4681 gtagccgaac atcttccggc gcacggccgc cggatggcgg gccgccgcgg catcgaagcg
     4741 ctc
//
LOCUS       DBNHXO010000110         5143 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1813271, whole genome shotgun sequence.
ACCESSION   DBNHXO010000110 DBNHXO010000000
VERSION     DBNHXO010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1813271"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="AC162A_02295"
     CDS             <1..789
                     /locus_tag="AC162A_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR5009696.1"
                     /translation="MWLGELITEKGIGNGISFIIFAGIVSRAPSALQAFINSPDLARF
                     VLFGILGVAAVAVIIYIQEGQRRIPIQYASRVRGRRMYQGGQTFLPLRVNQAGVIPII
                     FAVSILLFPSQIASYFVNAGGIVGDIANWIVTWLSSGSWLYVVLYFLLTVGFTYFYTA
                     FTFKPDETAEQLRKNGGFIPGIRPGRPTQDYLAKVVTRITVAGALFLGIVAALPPFIA
                     LIQPQYQGLALGGTGILIVVSVVVETMKQVEAQLLIRQYEGFIR"
     gene            923..1612
                     /locus_tag="AC162A_02300"
     CDS             923..1612
                     /locus_tag="AC162A_02300"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR5009697.1"
                     /translation="MQVVILLGAPGAGKGTQAPLLADRLGVPIVATGDLFRAAVRDGT
                     PLGIEARQYMDAGQLVPDEITVRLLLDRLARADADGGVILDGFPRTDVQARALDDALA
                     SRDSSVRVAVLVDVPTEELIRRMSGRWVCRAAGHPYHEVFSPPHVAGICDLDGSELYQ
                     RSDDRPETIRARMTQQLGALGAVVDHYRASGVLRTVNGLQDIDEVAAEVDAVVAEVGL
                     RRPASGDPPNA"
     gene            1612..2403
                     /gene="map"
                     /locus_tag="AC162A_02305"
     CDS             1612..2403
                     /gene="map"
                     /locus_tag="AC162A_02305"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR5009698.1"
                     /translation="MHVTRKSKAEIAKMRSAGRIVAEVLALVEESLLPGVSTAEIDRA
                     AERHIRAAKGVPSFLNYLGGRRYDARDPHAYPASTCISIDNEIVHGIPGDREIKAGQL
                     VSVDVGVIYDDWHGDGARTFICGGPEATTAAAQGLVEATRLSLMAGIYAAQPGNFICD
                     ISGAVEDIATPSGYGIIRQYVGHGIGTSMHEDPQVPNFRSRSKGLKLEPGLCLAIEPM
                     LTLGSEATQTLADGWTVATWDGSLAAHFEHTIAVTADGPEILTTV"
     gene            2606..2824
                     /gene="infA"
                     /locus_tag="AC162A_02310"
     CDS             2606..2824
                     /gene="infA"
                     /locus_tag="AC162A_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR5009699.1"
                     /translation="MPKKDAIEVEGTVIEPLPNTMFAVELENGHRVLAHISGKLRMNF
                     IRILPGDRVRVELSPYDLTRGRITYRLK"
     gene            2836..2949
                     /gene="rpmJ"
                     /locus_tag="AC162A_02315"
     CDS             2836..2949
                     /gene="rpmJ"
                     /locus_tag="AC162A_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006042225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR5009700.1"
                     /translation="MKVRASVKTRCDNCKVIRRHGTVMVICSNPKHKQRQG"
     gene            2954..3328
                     /gene="rpsM"
                     /locus_tag="AC162A_02320"
     CDS             2954..3328
                     /gene="rpsM"
                     /locus_tag="AC162A_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR5009701.1"
                     /translation="MARIAGIDIPREKRVEIALTYIYGVGLSSSQKILKQTSINPDTR
                     VRDLTEDQVNRLREVIDRSFKVEGDLRREVALNIKRLIEIGSYRGLRHRRNLPVRGQR
                     TKTNARQRRGPKKTVGARKKTK"
     gene            3346..3741
                     /gene="rpsK"
                     /locus_tag="AC162A_02325"
     CDS             3346..3741
                     /gene="rpsK"
                     /locus_tag="AC162A_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR5009702.1"
                     /translation="MAERKRAVKQRRRERKNVPQGHVHIQASFNNTIVTITDQGGNVI
                     SWGSAGIAGFKGSRKSTPYAAALAADGAAKRAMEHGMRQVEVYVKGPGAGREQAIRSL
                     QAAGLEVSAITDVTPIPHNGCRPPKRRRV"
     gene            3755..4381
                     /gene="rpsD"
                     /locus_tag="AC162A_02330"
     CDS             3755..4381
                     /gene="rpsD"
                     /locus_tag="AC162A_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR5009703.1"
                     /translation="MARYTDAVCRLCRREGLKLYLKGSRCYTKKCSFERRPTPPGQHG
                     VRRRKVGDYGLQLREKQKVRRVYGVLEKQFRNYYLEASRHSGVTGEALLRTLELRLDN
                     GVYRMGFAPSRAAARQMVAHGHFAVNGVPTNVPSYRLKIGDKVEVRKSHHERELFKVV
                     KETIRSHQAPDWLSLDAANLAGSVVAMPRRDQMPLDFNEQLVVEYYSR"
     gene            4392..>5143
                     /locus_tag="AC162A_02335"
     CDS             4392..>5143
                     /locus_tag="AC162A_02335"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR5009704.1"
                     /translation="MIELETPQIEPIAEAGTYAKYEAGPLQAGYGVTLGNALRRVLLS
                     SLEGAAVTSIQVRDVYQEFSTIPGVKEDVTQIVLNVKKLRLKSFATHPVQLRLIKSGA
                     GPVIAADIAESADVEIVNPELVLMTIDSDDVTIEMDLTVERGVGYLAAERSEALPIGV
                     IAVDAIFTPVRKVNYWVEGMRVGQMTNFDKLTIEIETDGTITPEEALSRSADILVRQF
                     QPFVTIGVQAVAVDRAPSGVPQLPPNMLDMPI"
BASE COUNT          877 a         1816 c         1671 g          779 t
ORIGIN      
        1 atgtggctgg gcgagctgat caccgagaag gggatcggca acggcatcag cttcatcatc
       61 ttcgcgggca tcgtcagccg cgccccgagc gcgctccagg cgttcatcaa cagccccgac
      121 ctcgcccgct tcgtgctgtt cgggatcctg ggggtcgcgg cagtcgccgt gatcatctac
      181 atccaggagg gccaacgccg gatcccgatc caatacgcgt cgcgcgtccg gggccggcgc
      241 atgtaccagg gcggtcagac gttcctgccc ctgcgcgtca accaggccgg cgtgatcccg
      301 atcatcttcg cggtcagtat cctgctgttc ccgagccaga tcgcgagcta cttcgtgaac
      361 gccggcggca tcgtcggcga catcgccaac tggatcgtga cgtggctcag cagcgggtcc
      421 tggctgtacg tcgtgctgta cttcctgctc accgtcgggt tcacgtactt ctacacggcg
      481 ttcacgttca agcccgacga gaccgccgag cagctgcgca agaacggcgg gttcatcccg
      541 gggatccgcc ccggacggcc cacccaggac tacctggcca aggtcgtcac gcggatcacc
      601 gtggctggcg cgctgttcct gggcatcgtc gccgcgctgc caccgttcat cgcgctgatc
      661 caaccgcagt accagggtct cgcgctcggt ggcacgggca tcctgatcgt ggtctcggtc
      721 gtggtcgaaa ccatgaaaca ggtcgaagcc cagttgctca tccggcaata cgagggattc
      781 atcaggtgac gggcaccgcc gtgacgggcg atgcgggcgt tgccgactcc gcgcgctcgc
      841 tcacgcaccg tcaggtgcgc tcgctcgtgt gctcgtcggc gccttcgcct cgccgcgtcc
      901 cggcggcgca gttcggctag ccatgcaggt cgtcatcctt ctgggcgctc caggcgctgg
      961 gaaggggacc caggcgcccc tgctcgccga ccgcctcggc gttccgatcg tggccactgg
     1021 cgacctgttc cgggccgccg tccgtgacgg gacgccgctg ggcatcgagg cccgccagta
     1081 catggacgcg ggtcagctcg tgcccgacga gatcaccgtc cgcctcttgc tcgaccgtct
     1141 cgccagggcg gacgccgacg gcggggtgat cctcgacggc ttcccccgga ccgatgtcca
     1201 ggcccgcgcg ctggacgacg ccctcgcgag ccgcgactcg tcggtccgcg tcgccgtcct
     1261 cgtcgacgtc ccgaccgagg agctcatccg ccgcatgtcc ggccgctggg tctgccgtgc
     1321 cgccggccac ccgtaccacg aggtgttcag cccgccgcac gtcgccggga tctgcgacct
     1381 cgacggctcc gagctctacc agcggtccga cgaccgcccc gaaaccattc gcgcgcgcat
     1441 gacccagcag ctgggcgcac tgggcgccgt cgtcgaccac taccgcgcgt cgggcgtcct
     1501 gcgcacggtc aacggcctgc aggacatcga cgaggtggcc gccgaggtcg acgccgtcgt
     1561 cgcagaggtc gggctccggc gccccgcgtc gggcgacccc ccgaacgcct gatgcacgtc
     1621 acccgcaagt cgaaggcgga gatcgcgaag atgcgatccg ccggccggat cgtggccgag
     1681 gtcctcgccc tcgtcgagga gagcctcctg cccggcgtct cgacggccga gatcgatcgc
     1741 gcggccgagc gccacatccg cgcggcgaag ggcgtgccgt cgttcctcaa ctacctcggc
     1801 ggccggcgct acgacgcgcg tgacccccat gcgtacccgg cctcgacctg catctcgatc
     1861 gacaacgaga tcgtccatgg catccccggc gatcgcgaga tcaaggcggg gcagctggtg
     1921 tcggtcgacg tcggcgtgat ctacgacgac tggcacggcg acggtgcgcg gacgttcatc
     1981 tgcggcggtc ccgaagcgac gaccgctgcg gcgcagggcc tcgtggaggc gacgaggctg
     2041 tcgctgatgg ccggcatcta cgcggcccag cccggcaact tcatctgcga catctccggc
     2101 gccgtcgagg acatcgccac gcccagcggc tacggcatca tccgccagta cgtcgggcac
     2161 gggatcggca cctcgatgca cgaggacccg caggtcccca acttccgctc gcgctcgaag
     2221 ggcctcaagc tcgagcccgg cctgtgcctg gccatcgagc cgatgctgac gctcgggtcg
     2281 gaggcgacgc agacgctcgc cgacggctgg acggtcgcca cgtgggacgg atcgctggcc
     2341 gcccacttcg agcacacgat tgccgtaacg gcggatggac ccgagattct gacgacggtc
     2401 tgagatcgat ggcaaggaga agcctgatct gtgatgaatc caccgacgac cgagctaagc
     2461 tcctgcgtgc gctcggtcgg aggtctcccg agatcctcac cacggtctag ggagttgtcg
     2521 aaagtgcgca gccttgatat cctcgccgtt cgtgtgtcgg cccgcccggg ccggcactcg
     2581 cgctgagagg acccaaggaa caggtttgcc gaagaaggac gcgatcgaag tcgaaggaac
     2641 cgtcatcgag cctctgccga acaccatgtt cgcggtcgag ctcgagaacg gccatcgtgt
     2701 tctcgcgcac atcagtggca agctccggat gaacttcatc cggatccttc cgggggaccg
     2761 ggtccgcgtg gagttgtctc cctacgacct cacccggggt cggatcacgt accgattgaa
     2821 gtaaacgagg aaccggtgaa ggtcagagcg tccgtcaaga cgcggtgcga caactgcaag
     2881 gtcatccggc gccacggcac cgtgatggtc atctgctcga atcccaagca caagcagcgg
     2941 cagggctgac ggcatggcac gtatcgccgg catcgacatc ccgcgcgaga agcgcgtcga
     3001 gatcgccctc acgtacatct atggggtcgg gctgtcctcc agccagaaga tcctcaagca
     3061 gacgagcatc aatcccgaca cgcgtgtccg ggacctcacc gaggaccagg tcaaccgcct
     3121 ccgcgaggtg atcgaccgga gcttcaaggt cgagggcgac ctccgccgcg aggtcgcgct
     3181 gaacataaag cggctgatcg agatcgggtc ctaccgcggg ctccgccacc gccggaatct
     3241 tccggttcgc gggcagcgaa cgaagaccaa cgcccgccag cgccgggggc ccaagaagac
     3301 ggtcggcgcg cgcaagaaga ccaagtgacg aggaaccgct aaggcatggc cgaacgaaag
     3361 cgcgccgtca agcagcggcg acgggagcgg aagaacgttc cgcagggaca cgttcatatc
     3421 caggcgagct tcaacaacac gatcgtgacg atcaccgatc agggtggcaa cgtcatctcg
     3481 tggggttccg cgggcatcgc cgggttcaag ggctcccgca agagcacacc gtacgccgcg
     3541 gccctcgccg ccgacggcgc cgcgaagcgc gccatggagc atggcatgcg ccaggtcgag
     3601 gtctacgtca agggacccgg cgccggccgc gagcaggcca tccgctccct ccaggccgcg
     3661 ggcctcgagg tctccgcgat caccgatgtc acccccatcc cgcacaacgg ttgccgcccg
     3721 cccaagcggc gccgcgtctg agcacgaaga gaccatggcc cgatataccg atgccgtctg
     3781 ccggctctgc cgccgggaag gactgaagct ctacctcaag ggcagccgct gctacaccaa
     3841 gaagtgctcg ttcgagcggc gtccgacgcc gcccggccag cacggcgtcc gccgccgcaa
     3901 ggtcggcgat tacggcctgc agttgcgcga gaagcagaag gtccgccgcg tctacggcgt
     3961 cctcgagaag cagttccgca actactacct cgaagcgtcg cgccactctg gcgtgaccgg
     4021 cgaggccctg ctgcggacgc tcgagctgcg cctcgacaac ggcgtctacc gcatgggctt
     4081 cgcgccctcg cgcgcagcag cccgccagat ggtcgcccac ggccacttcg ccgtgaacgg
     4141 cgtccccacc aacgtgccct cctaccggct gaagatcggc gacaaggtcg aggtgcgcaa
     4201 gtcgcaccac gagcgcgagc tgttcaaggt cgtcaaggag accatccgct cgcaccaggc
     4261 gccggactgg ctcagcctcg acgcggcgaa cctcgccggg tcggttgtgg ccatgccgcg
     4321 tcgcgaccag atgccgctcg acttcaacga gcagctcgtg gtcgagtact actcgaggta
     4381 gccgccgttc catgatcgaa ctcgagacac cgcagatcga gcccatcgca gaagccggca
     4441 cgtacgccaa gtacgaggct ggcccgctgc aggcgggcta cggcgtgacc ctcggcaatg
     4501 cgctccggcg cgtgctcctc tcctcgctgg agggggccgc ggtcacgtcc atccaggttc
     4561 gggacgtgta ccaggagttc tcgacgatcc cgggcgtcaa ggaagacgtc acgcagatcg
     4621 tgctgaacgt caagaagctg cgcctgaaga gcttcgcgac gcacccggtg cagctgcgcc
     4681 tgatcaagag cggtgccggc ccggtcattg ccgcggacat cgccgagtcg gccgacgtcg
     4741 agatcgtcaa ccccgagctc gtgctcatga cgatcgactc ggacgacgtg acgatcgaga
     4801 tggacctcac cgtcgagcgt ggcgtcggtt acctcgccgc ggagcgcagc gaggccctgc
     4861 cgatcggcgt catcgcggtc gacgcgatct tcaccccggt ccgcaaggtc aactactggg
     4921 tcgagggcat gcgggtcggt cagatgacca acttcgacaa gctcacgatc gagatcgaga
     4981 ccgacggcac gattacgccg gaggaggcgc tctcgcgctc cgccgacatc ctcgtccgcc
     5041 agttccagcc gttcgtgacg atcggcgtcc aggccgtcgc cgtggaccgc gcgccgtccg
     5101 gcgtgccgca gctcccgccg aacatgctcg acatgccgat cga
//
LOCUS       DBNHXO010000111         2759 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3201782, whole genome shotgun sequence.
ACCESSION   DBNHXO010000111 DBNHXO010000000
VERSION     DBNHXO010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3201782"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="AC162A_02340"
     CDS             complement(<1..128)
                     /locus_tag="AC162A_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR5009705.1"
                     /translation="MAKKKFERNKPHVNVGTIGHIDHGKTSLTAAITKYLALKGDA"
     gene            complement(195..270)
                     /locus_tag="AC162A_02345"
     tRNA            complement(195..270)
                     /locus_tag="AC162A_02345"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(235..237),aa:Thr,seq:ggt)
     gene            complement(322..397)
                     /locus_tag="AC162A_02350"
     tRNA            complement(322..397)
                     /locus_tag="AC162A_02350"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Thr,seq:tgt)
     gene            complement(467..1417)
                     /gene="rlmB"
                     /locus_tag="AC162A_02355"
     CDS             complement(467..1417)
                     /gene="rlmB"
                     /locus_tag="AC162A_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR5009706.1"
                     /translation="MPFRPVEPLAPSLTLAEDEELVAGRRPVEEAFVARREARRLLVV
                     PQRRRALEKLVLHATSLRIPVVEVEGGTLTQVAGFDGHQGVALVVGLRQWAAPDDILA
                     LASQRGEPPLVLVLDSLEDPQNVGTLLRTAEASGVHGAIFPTHHQAPLTPAAIKASAG
                     AVEHLRLAPVDDLPEALADLHVRGLRIVGADGDAALTAREADFRGPVAIIVGSEGKGL
                     GPAVRRRCDLLVRIPMHGKIDSLNAAVAGSILLYEAAAQRGLGGRPDAAPDEPMAPAA
                     EVVAEPADEPPVEPSNEPSETPSTALGEPAAEPPEETPEV"
     gene            complement(2130..>2759)
                     /locus_tag="AC162A_02360"
     CDS             complement(2130..>2759)
                     /locus_tag="AC162A_02360"
                     /inference="COORDINATES: protein motif:HMM:NF020752.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysS/YqeB C-terminal domain-containing protein"
                     /protein_id="HIR5009707.1"
                     /translation="HLRMDGEKMAKSTGNIARIGELVDSGVSPRAIRYALISVHYRAP
                     LNFSEESLTAAAAAVDRIDAVFAALAAYREDRDDDPSLPAALDAIRAGFDAGLDDDLN
                     VSAALGSLFEGIRDLNRRIDARALSTADAARATALLRDVDAVLGVAAPAEAALDPALQ
                     ALLDAREAARAARDWAASDRLRDELLAQGVAVEDTRDGQRWRLLVTSGG"
BASE COUNT          340 a         1011 c         1050 g          358 t
ORIGIN      
        1 tccgcgtcgc ccttgagggc gaggtacttg gtgatcgcgg ccgtgagcga cgtcttgccg
       61 tggtcgatgt ggccgatcgt gccgacgttc acgtgcggct tgttgcgctc gaacttcttc
      121 ttggccacag gaccttctca ctccaaggct ggggcggcgc aggccgccgg ttgtcgaccg
      181 ggatcgacga gagatggagc ccagaacggg attcgaaccc gtgacctcat ccttaccaag
      241 gatgtgctct gccagctgag ctatctgggc ccgcggagac cgcgccccgc ggcgcgcctc
      301 ctggcgcatt tccgttacgg atggagccga cgacgggact cgaacccgga acctgcggtt
      361 tacaaaaccg ctgctctacc aattgagcta cgtcggcacg cacgcagcgt caaacaagag
      421 ttggactttg cgggccgggg aatgatacgg cccggaagcg ggcgcgtcaa acctcgggcg
      481 tctcctcggg gggttccgcg gcgggctcgc cgagcgccgt ggacggggtc tcggacggct
      541 cgttcgaggg ctcgacaggc ggctcgtccg cgggctcggc gacgacctcc gcggcgggcg
      601 ccatcggctc gtcgggtgcg gcgtcgggcc gtccgcccag gcctcgctgg gccgcggcct
      661 cgtagagcag gatcgagccg gccacggccg cgttgagcga gtcgatcttg ccgtgcatcg
      721 ggatcctgac cagcaggtcg cagcggcgcc ggaccgccgg gccgaggccc ttgccctcgc
      781 tcccgacgat gatcgccacg ggcccccgga agtcggcctc gcgcgcggtg agcgcggcat
      841 cgccgtccgc cccgacgatc ctcagacccc ggacgtggag gtcggcgagc gcctccggca
      901 ggtcgtcgac tggggcaaga cgaaggtgtt cgaccgcgcc ggccgacgcc ttgatcgcgg
      961 ccggcgtgag cggcgcctgg tggtgggtcg ggaagatcgc cccgtgcacg cccgacgcct
     1021 ctgccgtgcg caggagcgtg ccgacgttct ggggatcctc gagcgagtcg aggaccagca
     1081 cgagtggcgg ctcgccacgc tggctggcga gggcaaggat gtcgtcgggc gcggcccact
     1141 ggcgaagccc gaccacgagc gcgacgccct ggtggccgtc gaaacccgcc acctgcgtca
     1201 gcgtcccgcc ctcgacttcg acgaccggga tccgcaggct cgtcgcgtgg agcacgagct
     1261 tctcgagcgc ccggcggcgc tgcgggacga cgagcagccg ccgagcctcg cggcgggcga
     1321 cgaacgcctc ctcgacggga cgccggcccg cgacgagctc ctcgtcctcg gcgagcgtca
     1381 gcgacggggc gagcggctcg accggccgga acggcaccgc cgcgtacgga cggacaggcg
     1441 gcccggacgg gcggtcgaag ggcgcgtcgg gccgatgcgg tggcgtcgaa tagcgacccg
     1501 cggggccctg gtggcggccc ggtgggcctg gccggaaccc gggcgaggcc gggcgataac
     1561 cctgcgggcc gggtcgcgca ccgggcgggc cgggacgata tcccggcggc cccggccggt
     1621 acccgggcgg acccggtcgg tacccggggg gtcctgagcg gtagccgggt ccggcgtgac
     1681 ggccggcctg cggcgggcga ccacccgcac gagcgtcgcg cggcggaccg tagtcgcggg
     1741 gggcatgccc aggaccggtg tcgcggcgcg gaccctcgtc gtcccagggc ctcggcggcc
     1801 gcggtccggt cggaccgcga cgaggatcgt cgttgtcgcg gcgcggcccg cgatcctgct
     1861 cgggcgctcc ccggaacccc gtcccccgcc gatcgccacc cggcccggac cctcgatatg
     1921 gcgcgccgcg ggacggcggc ccggcgtggg agcgcccgcc ctgcccgtgt gcgccgggcc
     1981 ctccgcgcgg tcgcgaccca ccgcccgacg tcggcttcgg gcgcggtccc gggggcgtgt
     2041 catgggcgcc gtcgcccgcg ggtgtgccgg cctctccgtc ccgagacggc ttcagcgtct
     2101 tcggctggcg gtcgcgcggg cgcggctggt cagccaccgg acgtcaccag cagccgccag
     2161 cgctgtccgt cgcgggtgtc ctcgacggcc acgccttggg ccagtagctc gtcgcgcagc
     2221 cggtcggacg cggcccagtc gcgcgccgcc cgcgcggcct cacgcgcatc gagcagcgcc
     2281 tgcagcgcgg ggtcaagcgc cgcttccgcc ggcgctgcga ccccgagcac ggcgtccacg
     2341 tcgcgcagca gggcggtcgc ccgcgccgca tcggccgtcg acagcgcgcg ggcatcgatc
     2401 cggcggttca ggtcgcggat cccctcgaac agcgagccca gcgccgcgga cacgttgagg
     2461 tcgtcgtcga gcccggcgtc gaagcccgcc cggatcgcgt ccagcgccgc gggcagcgac
     2521 gggtcgtcgt cccggtcctc gcggtacgcg gcgagggcgg cgaacaccgc atcgatccgg
     2581 tcgaccgccg cggccgcggc cgtcagggac tcctccgaga agttgagcgg ggcgcggtag
     2641 tggaccgaga tcagcgcata gcggatcgcg cgcggggaga cgcccgagtc caccagctcg
     2701 ccgatccgcg cgatgttgcc ggtcgacttg gccatcttct cgccgtccat ccgcaggtg
//
LOCUS       DBNHXO010000112         2314 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3400580, whole genome shotgun sequence.
ACCESSION   DBNHXO010000112 DBNHXO010000000
VERSION     DBNHXO010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3400580"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="AC162A_02365"
     CDS             complement(<1..1181)
                     /locus_tag="AC162A_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009708.1"
                     /translation="MTDATPRRGIGWQILAILAIGLVFRLIMAYGFDALRGSGFDSDL
                     GLFRFWASNLADHGPFGFYDRDFFADYTPGYLYALWLVGAVGELMGGVGDLIKLPAIL
                     TDVALGFIVYQMARDLGVSERRATIAGAVVVVNPITWFDSVVWGQVDSFGTVFLLLSI
                     RELWKARSERAAILAVVAALVKPQLAILVPIVAAVTIRRALWPDGGFGSESPPPASGF
                     GWERRTRGWIRIVSTGVAGFATAVALSAPFGLSVVSFTGSAPFVDSSLVRLILSTAST
                     YSYVSVNAYNLWALFPVDGESMATSRGWIADAPTADATAWASIGPFPAAVVGGLLLAL
                     LLFVVVPVLVARRPDRLTILVGVAVLALAFFAVPTRVHERYLFPLFAIAAIPFAFSWR
                     WR"
     gene            1241..>2314
                     /locus_tag="AC162A_02370"
     CDS             1241..>2314
                     /locus_tag="AC162A_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR5009709.1"
                     /translation="MKVERSIAEAPEGPPSPQPGAGRLAAAIVGLLLVLGAFLLYTWS
                     NPEHYNQYNHFVWQADAFLQGKVSFDWPVPDGSDRPSNWYFQDIYPLFDADGQPTGQV
                     LLPFPPLPALVLLPFVALAGLTVDQEAIAIGLAAIGVGAAWWMLGGLRIRLAVRALTT
                     VLFATGTVWWWAAAVGSTWYLAHLVAVIAALLAVGVALRNDPRAAVDDVGEPSGPPPV
                     GGWWSHVWPLDGSQVLAGLLLGIAATARLPLVFAAPFFILVGGGGTQARRLVSAAVGG
                     VLPVAALLVYTQLTTGSWLHPGYDYQYQLEANGYPTLGYNPSWAVEDLRYIPQNLGIM
                     FGALPVVAPDVKPNTLGFGDVTVL"
BASE COUNT          356 a          832 c          810 g          316 t
ORIGIN      
        1 cgccagcgcc aggagaacgc gaacgggatc gccgcgatgg cgaacagcgg gaacaggtag
       61 cgctcgtgga cgcgggtggg gaccgcaaag aacgcgagcg ccaggacggc gacgccgacg
      121 aggatcgtga ggcggtcggg tcgtcgcgcg acgaggaccg ggacgaccac gaacaggagc
      181 agcgcgagca gcagcccgcc gaccaccgcg gccgggaacg gtccgatcga cgcccaggcg
      241 gtcgcgtcgg cggtgggcgc gtcggcgatc cagccccggc tggtggccat gctctcgccg
      301 tcgacgggga acagggccca caggttgtag gcgttgaccg acacgtacga gtacgtcgag
      361 gcggtgctga ggatcagccg caccagcgac gagtccacga acggcgcgct gccggtgaac
      421 gacacgaccg acaggccgaa cggtgccgaa agcgcgaccg ccgtcgcgaa gccggccacg
      481 ccggtggaga cgatgcggat ccagcctctg gtccggcgct cccagccgaa ccccgacgcc
      541 ggcggcgggg attcgctccc gaacccgccg tccggccaca gcgcgcgccg gatcgtgacg
      601 gcggcgacga tcgggacgag gatcgccagc tgcggcttca cgagagccgc aacgacggcg
      661 aggatcgccg cccgctcgct gcgggccttc cacagctcgc gaatcgacag cagcaggaac
      721 accgtgccga agctgtcgac ctggccccag accaccgagt cgaaccaggt gatggggttc
      781 acgacgacga ccgcgccggc gatcgtggcc cgccgctcgc tgacgccgag gtcgcgcgcc
      841 atctggtaga cgatgaagcc gagggcgacg tccgtgagga tcgccgggag cttgatcagg
      901 tcgccgacac cgcccatgag ctcgccgacg gcgcccacca gccacagcgc atacaggtag
      961 ccgggggtgt agtcggcgaa gaagtcgcgg tcgtagaagc cgaacggacc gtgatccgcg
     1021 aggttgctcg cccagaaccg gaacaggccc aggtccgagt cgaaccccga accacggagt
     1081 gcatcgaacc cgtatgccat gatcagccgg aacaccaggc cgatcgccag gatcgccagg
     1141 atctgccagc cgatgcctcg ccggggggtc gcgtccgtca ttgtttcccg gccgggcgcg
     1201 tccgaaccca gaccggtcac ggggagcccc ctacaaagcg ttgaaggtgg agcggagtat
     1261 agccgaggcc cccgaagggc ccccctcgcc ccagcccggc gccggtcgcc tcgcggccgc
     1321 gatcgtcggg ctgctcctcg tgctgggcgc gttcctgctg tacacgtggt cgaatcccga
     1381 gcactacaac cagtacaacc atttcgtctg gcaggcggac gcgttcctgc agggaaaggt
     1441 ttcgttcgac tggcccgtcc ccgacggctc ggatcgcccg tcgaactggt acttccagga
     1501 catctatccg ctgttcgacg cggatgggca gccgaccggc caggtcctgc tcccgttccc
     1561 gccgctgccc gcgctggtgc tgctcccgtt cgtcgcgctg gcgggcctga ccgtcgacca
     1621 ggaggcgatc gcgatcggcc tcgcagcgat cggcgtggga gccgcgtggt ggatgctcgg
     1681 cggcctccgc atccggctcg ccgttcgtgc gctgaccacg gtgctgttcg cgacgggcac
     1741 ggtctggtgg tgggccgcgg ccgtcggcag cacctggtat ctcgcccacc tcgtcgccgt
     1801 gatcgccgcg ctgctcgccg tgggcgtcgc gttgcgcaac gacccgcgcg ccgccgtcga
     1861 cgacgtcggg gagccctcgg gcccgccacc cgtgggcggc tggtggtcgc acgtgtggcc
     1921 gctcgacgga tcccaggtgc tcgccgggct cctgttgggg atcgcggcca ctgcccgcct
     1981 gccgctcgtg ttcgcggctc cgttcttcat cctcgtgggc ggcggcggca cgcaggcgcg
     2041 acgcctcgtc tccgctgcgg tcggcggcgt gctccccgtg gccgccctgc tcgtgtacac
     2101 gcagctgacc acgggctcct ggctgcaccc cggctacgac taccagtacc agctcgaggc
     2161 caacggctac cccacgctgg gctacaaccc gtcgtgggcc gtcgaggacc tccggtacat
     2221 cccgcagaac ctcggcatca tgttcggcgc cctgccggtc gtggcgcccg acgtcaagcc
     2281 caacacgctt ggcttcggcg acgtgaccgt cctg
//
LOCUS       DBNHXO010000113         3215 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_425155, whole genome shotgun sequence.
ACCESSION   DBNHXO010000113 DBNHXO010000000
VERSION     DBNHXO010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_425155"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1094)
                     /gene="ku"
                     /locus_tag="AC162A_02375"
     CDS             complement(153..1094)
                     /gene="ku"
                     /locus_tag="AC162A_02375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end joining protein Ku"
                     /protein_id="HIR5009710.1"
                     /translation="MWKGAIQFGLVTIPVKLYTATESEYTIRFNMLHETDLSRIQMKV
                     WCPEDEQVISRGETVKGYEYAPDQYVVITDEDLEKVPLKTVRSIEIEQFAPRDAATDE
                     HAKFTKQSYYIEPDKIGRKAFYLLRQVLEERGLTAICKVVIRDREALASLDPFEGTML
                     LTTLHWPDEIRSTRDLDLPAEEFEFKPAELKMAEQLVETMTESFDPSKYKDEYREALL
                     KVIEAKVEGVEIATPEPVEESANLVDLMKLLEASVKAATDSKTAGATEPVNVADAKKA
                     REKRPAAKKATPKPAAAGPDEADDADSGRTTRRRKSA"
     gene            complement(1209..>3215)
                     /locus_tag="AC162A_02380"
     CDS             complement(1209..>3215)
                     /locus_tag="AC162A_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5009711.1"
                     /translation="SAAQFAALRADPNVEALVPDQVIRLEAQTTPRGVRRVFGPKNPI
                     ARIDGIDQRVDADVAIVDTGIDKDHGDLNVVGGVNCSSSNASAWGDGNGHGTHVAGIV
                     GAIDNGTGAVGVAPGVRLWAVRILTSGGSGLVSWYVCGLDWITAQRDPVDPSRPLIES
                     VNMSVAKAGKDDHNCGQTNHDPIHRAICRLVASGVTVVAAAGNNSFNASRLVPASYNE
                     VITVSALADSDGRPGGKGGRACYSWGSYDRDDTFANFSNYGADVDLIAPGKCIWSTVP
                     GGYGYLSGTSMAAPHVAGAVALYKASRPTATPVQVRHALEAFGNRDWKLSTDPDRYHE
                     PLLDVSRIVLLGDFAIAAPSPSKVFGADGGTVKVRVEAIRAEDVPDAIDLSVDADAPL
                     GASLSDDLLSGFDDSTSQLTVSVPPGTSSGTHFVDVTGRIGGTAHTTRVAIVVDTVAP
                     TIDRPWLGIVGDTIYSGGTFRASARWSAARDTTTSIRAYQVQWSSNGGSWGRTAELSS
                     AARAAGHSFAVGRSYRVRVRARDAAGNWSAWSQAGPFAAATVQDSSSTLSKSGKWHVS
                     RSRSWTGGTTRYAKASGASISREFTGRGVALVAPSGPKRGAARIYIDGNLARTIHLNG
                     KRLHPRRIAFTRTWTSTESHTIRVVVVGSPHHRRVDVDAFIVLL"
BASE COUNT          430 a         1106 c         1147 g          532 t
ORIGIN      
        1 cgagggcgac gacatcccgc gcgacatgcg ggtctcggtc cgggccgcgc ggatcgccat
       61 ggcggcgctg tcgccgttca cgggacggcg actgcgcgtg gtggtcatgc ggcgcgacga
      121 gccctgacgg ccgccggccg ggacagcctg gcctaggcgc tcttgcgacg cctcgtcgta
      181 cgcccgctgt ccgcgtcgtc ggcctcgtcg ggtccagcgg ccgccggctt cggagtcgcc
      241 ttcttcgcgg ccggccgctt ctcgcgggcc ttcttcgcgt cggcgacgtt gacgggctcg
      301 gtggcgccgg cggtcttcga gtcggtggcc gccttgacgc tcgcctcgag cagcttcatc
      361 aggtcgacga ggttcgcgct ctcctcgacc ggctccggcg tcgcgatctc gacgccctcg
      421 accttggcct cgatcacctt gagcagggcc tcgcggtact cgtccttgta cttggacggg
      481 tcgaaggatt cggtcatcgt ctcgaccagc tgttcggcca tcttgagctc ggcgggcttg
      541 aactcgaact cctcggccgg caggtcgaga tcgcgggtcg agcggatctc gtctggccag
      601 tggagggtcg tgagcagcat cgtgccctcg aacgggtcga gcgaggccag cgcctcgcga
      661 tcccggatca cgaccttgca gatcgccgtg agcccgcgct cctcgagcac ctggcgcagc
      721 aggtagaagg ccttgcggcc gatcttgtcg ggctcgatgt agtaggactg cttggtgaac
      781 ttcgcgtgct catccgtggc tgcgtcgcgc ggggcgaact gctcgatctc gatcgagcgc
      841 acggtcttga gcgggacctt ctcgaggtcc tcgtcggtga tcacgacgta ctggtcgggc
      901 gcgtactcgt agcccttcac ggtctcgccg cgcgagatca cctgctcgtc ctccgggcac
      961 cagaccttca tctggatcct cgagaggtcc gtctcgtgca gcatgttgaa ccggatcgtg
     1021 tactcagact cggtcgctgt gtacagcttg accgggatgg tgaccagtcc gaactggatg
     1081 gcccctttcc acatcgagcg tgccacggta ttgctccgtc ctcgcgtcgc ggccacccga
     1141 gcggctcccg ttgccgccgt cgaccggagc gtgaccatac cacccggtcg caatcgtgcc
     1201 gcgcggccct agaggaggac gatgaacgcg tccacgtcga cccggcgatg atggggtgac
     1261 ccgacgacca cgacccggat cgtgtgggac tcggtggacg tccaggtgcg cgtgaacgcg
     1321 atccgccgcg ggtgcaggcg ctttccgttg aggtggatcg tgcgggcgag gttgccgtcg
     1381 atgtagatcc gggcggcgcc acgcttggga ccactgggcg caacgagggc gacgccacgg
     1441 cccgtgaact cgcggctgat cgacgcgccc gacgccttcg cgtagcgggt cgttccgccg
     1501 gtccacgatc gcgagcggga gacgtgccac ttgcccgact tgctgagggt cgagctggag
     1561 tcctggacgg tcgccgctgc gaacggcccg gcctggctcc acgcgctcca gttgccggcg
     1621 gcatcgcgcg cgcggacccg gacccgatag ctgcgcccga cggcgaagct gtgccccgcg
     1681 gcgcgcgccg ccgagctgag ctcggccgtg cgcccccacg agcccccgtt ggagctccat
     1741 tggacctggt aggcgcggat gctcgtcgtg gtgtccctcg cggccgacca gcgtgccgac
     1801 gccctgaagg tgccgccgct gtagatcgtg tcaccgacga tccccagcca cggccggtcg
     1861 atcgttggcg cgaccgtgtc cacgacgatc gccacccgcg tggtatgcgc ggtgccgccg
     1921 atccgcccgg tgacgtcgac gaagtgggtc ccgctcgacg tgccgggcgg cacggacacg
     1981 gtgagctgcg aggtcgagtc gtcgaacccc gacaggagat cgtccgagag cgacgcgccg
     2041 agcggcgcat cggcgtcgac ggagaggtcg atggcgtccg gcacgtcctc ggcgcggatc
     2101 gcctccacgc ggaccttgac ggtcccgccg tccgcgccga acacctttga cggcgagggt
     2161 gcggcgatcg cgaagtcacc cagcagcacg atgcgggaca cgtcgagcag cggttcgtgg
     2221 taccggtccg ggtcggtcga gagcttccag tcgcggttgc cgaacgcctc gagcgcgtgg
     2281 cggacctgga ccggcgtcgc cgtcgggcgc gaagccttgt agagcgcgac ggcgcccgcg
     2341 acgtgtggcg ccgccatcga cgtgccggac aggtagccgt acccgccggg cacggtcgac
     2401 cagatgcact tgccgggcgc gatcaggtcg acgtccgccc cgtagttgga gaagttcgcg
     2461 aacgtatcgt ccctgtcgta gctgccccag gagtagcagg cgcggccgcc cttgccgccc
     2521 ggacggccgt cactgtcggc gagggccgag acggtgatga cctcgttgta gctcgccggg
     2581 accagccgcg acgcgttgaa gctgttgttg cccgcggcgg cgaccacggt cacccccgag
     2641 gcgacgaggc ggcagatcgc gcggtggatc gggtcatggt tggtctggcc gcagttgtgg
     2701 tcgtccttgc ccgccttggc gaccgacata ttcaccgact cgatgagcgg gcggctcggg
     2761 tcgaccgggt cgcgctgggc ggtgatccag tcgaggccgc agacgtacca cgacaccagt
     2821 cccgacccgc cggaggtgag gatccgcacg gcccacaggc ggacgccggg ggcaacgccg
     2881 acggcgccgg tgccgttgtc gatcgccccg acgatgccgg cgacatgggt cccgtgcccg
     2941 ttgccgtcgc cccaggccga ggcgttggag ctggagcagt tgacgccgcc gaccacgttg
     3001 aggtcgccgt ggtccttgtc gatcccggta tccacgatcg cgacgtcagc gtccacgcgc
     3061 tggtcgatgc cgtcgatccg ggcgatcggg ttcttgggtc cgaacacacg gcgcacgcca
     3121 cgcggggtcg tctgggcctc gagcctgatg acctggtcgg gcaccagcgc ctcgacattg
     3181 gggtcggcgc gcaacgccgc gaactgggcc gcgct
//
LOCUS       DBNHXO010000114         2568 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_622625, whole genome shotgun sequence.
ACCESSION   DBNHXO010000114 DBNHXO010000000
VERSION     DBNHXO010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_622625"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="AC162A_02385"
     CDS             <1..1018
                     /locus_tag="AC162A_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR5009712.1"
                     /translation="TVVALARQAGIVDAASIAGHDLDDLDEVALGKVAEDVTVFGRIT
                     PAQKERLVGALRARGHYVAMIGDGVNDVLSLKKANLAIAMGSGSQATRGVADLVLMED
                     SFGAVAQAVQEGQRILNGMEDILKLFLTRIATVGLVIVSSLVVITFPIELRNASALTV
                     FTVGIPSALLALWAQPGAQVRDSIDRTLARFVVPAAVVSSLVGLLVFYGVLALSNPDP
                     SAIEGAAGNARTALTSFLVFVGLFLIVFVEPPIRWFAVAEPITRDRRPTYLAIGLGFA
                     FVAVLLLPPARAFFQLIVPAPRDALIVILAVVAWVLLVRTFWAYRVVDRFLGRAPTRS
                     EATG"
     gene            1015..1374
                     /locus_tag="AC162A_02390"
     CDS             1015..1374
                     /locus_tag="AC162A_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5009713.1"
                     /translation="MTLRMTEVVIDCGDHGEVVDFWAAAMGYERREVNEQYVALVPPA
                     PAELGRPPLLFQKVPEPKVAKNRVHLDLRGDVMADEVARLVGLGASVVAERSLGSLSW
                     TVMADPAGNEFCVSGQG"
     gene            complement(1378..>2568)
                     /locus_tag="AC162A_02395"
     CDS             complement(1378..>2568)
                     /locus_tag="AC162A_02395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR5009714.1"
                     /translation="WVQPESPVAHALTPDRSIVLERDLADHGAALEEALGRDAAAWER
                     LFGHLAGESERLLPSLLRPVVRPPRHPILMARFGLPAMLPAASLARLAFREPAARALF
                     AGLAAHSMLRLGQPLSASFGLVLGLLAHAVGWPLARGGSGRIAAALEAEARSLGVEIE
                     TGRRIEALGELPPARAVLFDLTPRQVLAIAGDRLPPGYRRQLAGFRYGPGVHKVDWAL
                     DGPVPWRDPGTSRAGTVHLGGPMRDIAASEDAVHRGRVSDRPFVLLVQPTIADPSRAP
                     AGKHVAWAYCHVPHGWDGDATGAIEAQVERYAPGFRDLVLGRSVRDAAAIEAYDANYI
                     GGDINGGVQDVRQLLFRPVVRWNPYTTPDPGLYLCSSSTPPGGGVHGMSGTHAARAAL
                     RATR"
BASE COUNT          331 a          933 c          937 g          367 t
ORIGIN      
        1 cacggtcgtc gcgctcgcgc gccaggccgg catcgtcgac gccgcgtcga tcgcgggtca
       61 cgacctggac gacctcgatg aggtggccct gggcaaggtc gccgaggatg tgacggtctt
      121 cggccggatc acgccggccc agaaggagcg actcgtcggc gcgctccggg cccgcggtca
      181 ctacgtggcg atgatcggcg acggcgtgaa cgatgtcctc tcgctcaaga aggccaacct
      241 cgcgatcgcg atggggagcg gcagccaggc gactcgcggc gtcgccgacc tcgtgctgat
      301 ggaggactcg ttcggggcgg tcgcccaggc ggtccaggag ggccagcgga tcctcaacgg
      361 catggaggac atcctcaagc tgttcctgac ccggatcgcc acggtcggcc tcgtgatcgt
      421 cagcagcctg gtcgtgatca cgttcccgat cgagctgcgg aacgcgtccg ccctgacggt
      481 gttcaccgtc gggatcccga gtgcgctgct cgcgctgtgg gcccagccgg gcgcccaggt
      541 gcgcgacagc atcgatcgga cgctggcccg cttcgtcgtc cctgccgcgg tcgtgtcgag
      601 cctcgtcggg ctgctggtgt tctacggcgt gctcgcgctg tccaatccag acccgtccgc
      661 gatcgaagga gcggccggca acgcccggac cgccctcacc tcgttcctgg tcttcgtcgg
      721 gctgttcctg atcgtgttcg tggagccccc gatccgctgg ttcgcggtcg ccgagccgat
      781 cacgcgcgac cgccggccga cgtacctcgc catcggtctg gggttcgcct tcgtggcggt
      841 cctcttgctg ccgccggccc gcgcgttctt ccagctgatc gtgccagcgc cgcgggatgc
      901 gctgatcgtg atccttgcgg tcgtcgcgtg ggtactcctg gtacggacct tctgggccta
      961 ccgcgtcgtg gaccggttcc tcgggcgggc cccaacgcga tcggaggcga cgggatgacg
     1021 ctgcgcatga ccgaggtggt gatcgactgc ggggaccatg gcgaggtcgt cgacttctgg
     1081 gccgccgcca tgggctacga gcggcgcgag gtcaacgagc agtacgtcgc gctcgtcccg
     1141 ccggccccgg ccgagctcgg ccgcccgccg ctgctgttcc agaaggtccc cgagcccaag
     1201 gtcgccaaga accgggtcca cctcgaccta cgcggcgacg tgatggcgga cgaggtcgcc
     1261 cgcctcgtcg gcctcggtgc gtcggtggtc gcggagcgtt cgctgggcag cctcagctgg
     1321 acggtgatgg ccgatcctgc cggcaacgag ttctgcgtct cgggccaggg ctgaggctca
     1381 gcgcgtcgcg cggagggccg ctcgggcagc gtgcgtcccg ctcatcccgt gcacgccccc
     1441 acccggcggc gtcgacgagg agcacaggta caggccgggg tccggcgtcg tgtacggatt
     1501 ccagcggacg accggccgga acagcagctg gcgcacgtcc tggacgccgc cgttgatgtc
     1561 gccgccgatg tagttggcgt cgtacgcctc gatcgccgcc gcgtcgcgca ccgatcggcc
     1621 gaggacgagg tcgcggaacc ccggcgcgta ccgctcgacc tgcgcctcga tcgcccccgt
     1681 cgcgtcgccg tcccagccgt ggggcacgtg gcagtacgcc cacgcgacgt gcttcccggc
     1741 aggcgcgcgc gacgggtctg cgatagtcgg ctggaccagg agcacgaacg gccggtcgga
     1801 cacgcgaccc cggtgcacgg cgtcctcgga cgccgcgatg tcgcgcatcg ggccgccgag
     1861 gtgcacggtc ccggcgcgcg atgtccccgg gtcgcgccag gggaccgggc cgtcgagcgc
     1921 ccagtcgacc ttgtggacac cgggcccgta gcggaaccct gccagctggc gccggtaccc
     1981 gggtggcagc cggtcgcccg cgatggccag gacctggcgc ggcgtgaggt cgaacagcac
     2041 ggctcgcgca ggcgggagct cgccgagcgc ttcgattcgc cgtcccgtct cgatctccac
     2101 gcccagcgac cgcgcctctg cctcgagcgc cgcggcgatc cgcccgctcc cgccccgcgc
     2161 gaggggccag ccgaccgcgt gcgcgagcag gcccagcacg aggccgaacg acgccgacag
     2221 cggctgcccc agccggagca tcgagtgggc cgcgaggccg gcgaacaggg cccgggccgc
     2281 cggttcgcgg aacgcgagtc gagcgaggct cgccgccggc agcattgccg gcaggccgaa
     2341 gcgggccatg aggatcgggt gacgcggggg gcggacgacg ggtcgcagca gcgacggcag
     2401 caggcgctcc gactcgcccg cgaggtggcc gaacagccgc tcccacgcgg ccgcgtctcg
     2461 cccaagcgcc tcctccagcg ccgctccgtg gtcggccaga tcgcgttcca gcacgatcga
     2521 gcgatccggt gtcagcgcgt gggcgaccgg ggactccggt tggaccca
//
LOCUS       DBNHXO010000115         1635 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1020846, whole genome shotgun sequence.
ACCESSION   DBNHXO010000115 DBNHXO010000000
VERSION     DBNHXO010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1020846"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="AC162A_02400"
     CDS             complement(<1..212)
                     /locus_tag="AC162A_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HIR5009715.1"
                     /translation="MSPVRVWILAARPPTLPAALSGVVVGLGAALGSGATFRLDTALG
                     CVAVALLLQIVANFANDLSDFRRGADT"
     gene            complement(209..1039)
                     /gene="menB"
                     /locus_tag="AC162A_02405"
     CDS             complement(209..1039)
                     /gene="menB"
                     /locus_tag="AC162A_02405"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015880852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HIR5009716.1"
                     /translation="MTAAVTWTPVVAYEDIRFEHSGTGIAKVTINRPEVRNAFRPQTV
                     RELIDAFARIRDDATVGCVLLTGEGDMAFCSGGDQKYKSHEGGYLDSDGLARLNVLDL
                     QRQIRSLPIPVIALVNGYAIGGGHVLHVVCDLSIASDNAIFGQVGPRVGSFDAGFGVG
                     LLARLVGDKKAKEIWFLCRQYSAPEALAMNLVNKVVPLADLEAEGVAWADEILQMSPT
                     AIRFLKSAFLVATDGLAGLQEFAGNATGLYYTTDEAHEGSTAFLEKRPPEYTKYPRRP
                     "
     gene            complement(1036..>1635)
                     /locus_tag="AC162A_02410"
     CDS             complement(1036..>1635)
                     /locus_tag="AC162A_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5009717.1"
                     /translation="ADDLATILRALDAAPASVLGYSLGARIALRLAIVHPDVVGRLVL
                     ESPSAGIVDNGDRETRRATDEALARRIERDGIEAFVAEWEAQPIFASQAALPADRAAR
                     IRRIRLANRPDGLAASLRGAGQGSMEPLVDRLAEVGAPTLVITGAFDDRARPRAEHVA
                     AGIPNARLAVVEGSGHTPHDEQPRRFRRLALDFLQEDAA"
BASE COUNT          225 a          535 c          653 g          222 t
ORIGIN      
        1 gtgtccgcgc cgcggcggaa gtcagagagg tcgttggcga agttggcaac gatctggagc
       61 agcagggcga ccgccacgca gcccagcgcc gtgtcgagcc tgaacgtagc cccggatccg
      121 agcgccgcac ccagccccac gacgaccccg gacagcgccg cgggcagggt cgggggacgg
      181 gcggcgagga tccagacgcg gaccgggctc acgggcggcg cgggtacttc gtgtactcgg
      241 gcgggcgctt ctcgaggaac gccgtcgaac cctcatgtgc ctcgtcggtc gtgtagtaga
      301 ggccggtcgc gttgcccgcg aactcctgga gcccggccag gccgtcggtg gcgacgagga
      361 acgccgactt gaggaaccgg atcgccgtcg ggctcatctg gaggatctcg tcggcccacg
      421 ccacgccctc ggcctcgagg tcggccagcg gcacgacctt gttcacgagg ttcatcgcga
      481 gcgcttcggg ggccgagtac tggcggcaca ggaaccagat ctccttggcc ttcttgtcgc
      541 cgacgaggcg agccagcagg ccgacgccga agccggcatc gaagctgccg acccgggggc
      601 cgacctggcc gaagatcgcg ttgtcgctcg cgatcgagag gtcgcatacg acgtgcagca
      661 cgtggccgcc gccgatggca tacccgttga cgagggcgat gaccgggatc ggcagcgagc
      721 ggatctggcg ctggaggtcg agcacgttga ggcgggcgag gccgtcgctg tcgaggtagc
      781 cgccctcgtg gctcttgtac ttctggtcgc cgcccgagca gaacgccatg tcgccctcgc
      841 cggtcagcag cacgcagccg accgtggcgt cgtcgcggat gcgggcgaag gcgtcgatca
      901 gctcccgcac cgtctgcggc cggaacgcgt tgcggacctc ggggcggttg atcgtcacct
      961 tcgcgatccc cgtgcccgag tgctcgaagc ggatgtcctc gtacgcgacg acgggcgtcc
     1021 aggtcacggc ggcggtcatg cggcgtcctc ctgcaggaag tcgagggcga ggcggcggaa
     1081 gcggcggggt tgctcgtcgt ggggggtgtg gcccgagccc tcgacgacgg cgaggcgggc
     1141 gttggggatg ccggcggcca cgtgctcggc acgagggcgg gcccggtcgt cgaaggcacc
     1201 ggtgatgacg agcgtcgggg caccaacctc ggcgagccgg tcgacgagcg gctccatcga
     1261 gccctggccg gcgccgcgca ggctggcggc gaggccgtcg ggccggttgg cgaggcggat
     1321 gcggcggatg cgggctgcgc ggtccgcggg gagcgcggcc tggctggcga agatcggctg
     1381 ggcttcccac tcggcgacga aggcttcgat gccgtcgcgc tcgatgcggc gggcgagggc
     1441 ctcgtcggtg gcgcggcggg tctcgcggtc gccgttgtcc acgatcccgg cggacgggct
     1501 ctccagtacc aggcggccga cgacgtcggg gtggacgatg gccagccgca gggcgatccg
     1561 ggcgccgagc gagtagccga ggacgctggc gggggccgcg tcgagggcgc gcaggatcgt
     1621 cgccaggtcg tcggc
//
LOCUS       DBNHXO010000116         6708 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2408963, whole genome shotgun sequence.
ACCESSION   DBNHXO010000116 DBNHXO010000000
VERSION     DBNHXO010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6708)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6708
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2408963"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(446..655)
                     /locus_tag="AC162A_02415"
     CDS             complement(446..655)
                     /locus_tag="AC162A_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009718.1"
                     /translation="MHPETLYELATMRIAEDHAYAARQRMAREAREARKEAQGANADP
                     SLLDRITTALRNAVRPVSRPTAGVA"
     gene            850..1293
                     /locus_tag="AC162A_02420"
     CDS             850..1293
                     /locus_tag="AC162A_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009719.1"
                     /translation="MTAAPGVLGDRPAPTGLPDPRIQPPTIAEAGDPFAAVRVIDLVA
                     RLDRGRAIRLDDVVDHLNAAYTDWLFTRAVVADALIALQANWMADYRNASGIEIEEGP
                     YGLAVTIEDSSRVDPWIVGQAQRAAAACRQALDAFARRDRVATGG"
     gene            1410..2276
                     /locus_tag="AC162A_02425"
     CDS             1410..2276
                     /locus_tag="AC162A_02425"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR5009720.1"
                     /translation="MTVTGYAKDDVLVSADWAKARLDDPSIRFVEVDVDTTAYDQSHI
                     PGAVGWNWTSQLSDGVRRDIATQADFSELLSASGIGPDTTIVLYGDNNNWFAAWAYWQ
                     LQLYGHTNVRLLNGGRKFWLDNNLPLSTDVPSYAATGYQLPDADFSLRAFRDDILPRV
                     GDASLQLVDVRSPAEFNGEVIAPPGMTETAQRAGHIPGAASIPWAQQVREDGTFKSAD
                     ELTALYAAKGITADRDTIAYCRIGERSSLSWFVLHELLGFSRVRNYDGSWTEWGSMVG
                     VPIEVSQPVAVG"
     gene            complement(2342..3082)
                     /locus_tag="AC162A_02430"
     CDS             complement(2342..3082)
                     /locus_tag="AC162A_02430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5009721.1"
                     /translation="MGPYVRHLIANHRVLVTTIDGDVVAYGAVVDAVRAAQLCDLFVD
                     PGRLGQGIGRPLLTALFEGSTHRTTFASDDPRALPLYARAGMSPRWVCLYLDGASSAI
                     EAQPGLDVESAEPTRIAELERVWTGTFRPVDHAFWATQAEADPFVVSDGAGPVAAGYG
                     RARQVSDARALDRLVVRPDADPVAPALEALRRIGRSGPVHAAMPGPNPLLQALLERRF
                     QVVDRDQYMASSDDIVDPIHMFPNTGLL"
     gene            complement(3190..4377)
                     /locus_tag="AC162A_02435"
     CDS             complement(3190..4377)
                     /locus_tag="AC162A_02435"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010446150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmpA family aminopeptidase"
                     /protein_id="HIR5009722.1"
                     /translation="MPARQLGIRIGRLEAGPNDAITDVRGVRVGHTTLIDGHGPMVVG
                     RGPVRTGVTVILPHGDDVWAEPVFSGCHRLNGNGELTGLEWIREAGLLGGPIAITNTH
                     SVGVVRDALVAHAIAHRRSGGGLWSLPVVGETWDGLLNDIDGLHVRPEHVDAAIASAT
                     GGPVAQGNVGGGTGMVCHEFKGGIGSASRVVAEADGGWTVGALVQANYGEREQLRVDG
                     VPVGIEIPDTSVPSPYDAAEAADDLRAAAGGGRAPAIEPGTGSIIVIVATDAPLRPHQ
                     CERLAQRATLGLARMGSTANHSSGDLVLAFATGNRGLSTTDDPGHAAPMTHEVRMVSD
                     NRMTPLFKATVEATEAAIVNALLAAETLSGRDDVTAHALDHERLLDVMARYGRGPRAA
                     GGT"
     gene            4498..5865
                     /locus_tag="AC162A_02440"
     CDS             4498..5865
                     /locus_tag="AC162A_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012640.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HIR5009723.1"
                     /translation="MPTLDGIAPMDAAEAFRDLPGLALLESARPGRTGRWSYLTADPA
                     AVVDAPADGADPFAEARSMLARLDGGGGGDSPGPPFAGGLVGYLGYDLGRRFERLPEI
                     ARVDQHLPMLRLALHDWVLAWDRRTGVAWLAARAVDGDVASLRRRMDEIQARVRASVA
                     REEPSAPSIPSTFVSLTQHDAWVRDVEAVRSAIGRGDIYQANLTRRLEAPFEDDPWPV
                     FRRLRTGDPALFAGFLDIGHSPANGRPRALLSASPEPFLSVDAQGVVSTDPIKGTRPR
                     GRTREQDRALARELLASPKDQAENVMIVDVLRNDLGRVCEPGSVRVPRLLRLERTAAV
                     QHLVTTVTGQLRPDADAFDLLASAFPGGSITGAPKIRAMELIERLEPVRRGPYCGTML
                     WLGPDGRMGSSILIRTFVADGERLTLHVGGGITWRSDPEAEWDETVAKARGPLSSVGA
                     VEVPG"
     gene            5886..>6708
                     /locus_tag="AC162A_02445"
     CDS             5886..>6708
                     /locus_tag="AC162A_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR5009724.1"
                     /translation="MWVDGALLPADGPHLSAFDRAFQLGDGIFETLRARGGRLTELTE
                     HTARLQRSADGLGIELPDDVAELLERAIRELLAAEGLDGADGDASVRVTVSRGAWMSR
                     GLLPPRDERLTATIVIQAWPVLAPPADHLSRGLSIVASSVRRDPQNPLAALKTTSRAD
                     YVFARLEARRAGGDDALFLTISGHLSEATTANIFLVRTSAEGVVELATPALDCAILAG
                     TTRSWLLRWGSQVGLRPVETWLTPEDLAAADEAFVCSSVAGVLPVTRFDGAPIGDG"
BASE COUNT          880 a         2463 c         2413 g          952 t
ORIGIN      
        1 aggcaacgcg gcgcgcccga cggcccgagg cgccggcgtc actgctgacc ggcccgcctg
       61 accgcgaccc gcggagaagc gggcgctcgc tgggtcgacg atccgcgatg cgcccgcgat
      121 ctccagctcc acgcgcatgg atcgggtcgc gggcgtcgcg actcgagact gcgtggctcg
      181 ccgcggccgc cggaggccgc ggcttgctcg tgatcggggg cgagggggga tcggcctgtc
      241 aaggcgcttg cgcgagctgg gccgccgctg tcggcgacca gggcggacgg tccgtgactg
      301 gagcgtgcat gcagctgggg gcggtcacca tcgaccggat ctgcaggtgc ccgtcatgac
      361 ctccgcggtc gcgcgccggg cgctcgcccg gaaacgaaga ggtgcgaccg cccgcgcatc
      421 ctgcgcttgc ggtcgcaccc agtgatcacg caacgccggc ggtcgggcgc gagaccggcc
      481 ggaccgcgtt ccgcagcgcc gtcgtgatcc ggtccagcag ggacggatcg gcgttggcgc
      541 cctgcgcctc cttgcgggcc tcacgggcct cgcgcgccat gcgctggcgg gcggcgtagg
      601 catggtcctc ggcgatccgc atggtcgcca gctcgtacag ggtctcggga tgcatggtcg
      661 ttctccaggg ttgcgaccga cgggtgtcgg cgatgagcgc agcatcgatt ccggaggctc
      721 gccggccatc gggcgggtgc ctgacgggat gggtgccggg tacctgaaac gcgcgtgtag
      781 gcaggccgga ggcgctgtgc tagccttcca aacccgacca ttccgctcgg atattcgatg
      841 accgcctcga tgaccgccgc gcccggggtg ctgggcgacc ggcctgcccc gacaggcctg
      901 cccgatccgc ggatccagcc gccgaccatc gccgaagccg gcgatccgtt cgcggccgtg
      961 cgcgtcatcg acctcgtcgc ccggctcgac cgcggacgcg cgatccgcct cgacgacgtc
     1021 gtcgaccacc tgaacgccgc gtataccgac tggctgttca cccgtgccgt cgtcgcggac
     1081 gccctgatcg ccctccaggc gaactggatg gcggattacc gcaacgcgtc ggggatcgag
     1141 atcgaggaag gtccgtacgg cctcgccgtc accatcgagg actcgagccg cgtcgatccc
     1201 tggatcgtgg gccaggccca gcgggctgcg gccgcgtgcc ggcaggcgct cgatgcgttc
     1261 gcacgtcgtg atcgcgtggc gaccggtggc tgacctgcat cagggccgct gcgcgtgtag
     1321 ggggcgggtc cggtccagct agaccgcgcg cccgccgacg catcgccccc tcatccgttc
     1381 tccccttccc aaccttggag cacccttcga tgaccgtgac cggctacgca aaggacgacg
     1441 tcctcgtctc ggccgactgg gcgaaagccc gcctggacga tccgtcgatc cgcttcgtcg
     1501 aggtcgatgt cgacaccacc gcctacgacc agagccacat ccccggagcc gtgggctgga
     1561 actggacaag ccagctgtcc gatggcgtgc gtcgcgacat cgcgacccag gccgacttca
     1621 gcgagctgct cagcgcgagc ggcatcggcc ccgacaccac catcgtcctg tacggcgaca
     1681 acaacaactg gttcgccgcc tgggcgtact ggcagctgca gctgtacggg cacaccaacg
     1741 tgcgcctgct caacggcggc cgcaagttct ggctggacaa caacctgccg ctctcgacgg
     1801 acgtgccgtc gtacgcggcg accggctacc agctgcccga cgccgacttc tcgctgcgag
     1861 cgttccgcga cgacatcctg ccgcgggtcg gcgacgcgtc gctgcagctg gtggacgtcc
     1921 ggagtcccgc cgagttcaac ggcgaggtca tcgcccctcc cggcatgacc gagaccgccc
     1981 agcgcgcagg gcacatcccg ggcgccgcgt cgatcccgtg ggcccagcag gtccgcgagg
     2041 acggcacgtt caagagcgcg gacgagctca ccgccctgta cgcggccaag ggcatcaccg
     2101 ccgaccgcga cacgatcgcg tactgccgga tcggcgagcg ctcgtcgctc agctggttcg
     2161 tcctccacga gctgctgggc ttctcgcgcg tccgcaacta cgacggctcc tggaccgaat
     2221 ggggatcgat ggtgggcgtg cccatcgagg tctcccagcc ggtcgcggtc gggtaacgcc
     2281 ggccacccgt accgggggga tccagcggcg ccggggcaac ccggcgccgt ttcgattcgg
     2341 gctacagcag ccccgtgttg gggaacatgt ggatcgggtc gacgatgtcg tcggacgacg
     2401 ccatgtactg gtcgcggtcc acgacctgga accggcgctc gagcagcgcc tggagcagcg
     2461 ggttgggccc gggcatggcg gcgtggacgg ggccgctacg gccgatgcgt cggagcgcct
     2521 cgagggcggg ggcgacagga tccgcgtccg gccgcacgac gagccggtcg agcgcccggg
     2581 catcagatac ctggcgggca cgaccgtagc ccgcggcgac cggccccgca ccgtccgaca
     2641 cgacgaacgg atcggcctcg gcctgcgtcg cccagaacgc gtggtcgacg gggcggaacg
     2701 tgcccgtcca tacgcgctcg agctcggcga tccgagtcgg ctccgccgac tccacgtcga
     2761 gcccgggctg ggcctcgatc gcgctcgagg cgccgtcgag gtacaggcac acccagcgcg
     2821 gcgacatccc ggcccgcgca tacagcggca gcgcgcgcgg atcgtccgag gcgaacgtcg
     2881 tccggtgcgt cgagccctcg aacagcgccg tcagcagcgg ccggccgatg ccctggccca
     2941 agcgccccgg atcgacgaac aggtcgcaca gctgggcggc gcgcacggcg tcgaccaccg
     3001 cgccgtacgc gacaacatcg ccgtcgatgg tcgtgaccag gacccggtgg ttggcgatga
     3061 ggtgacggac gtacggcccc acgatgtccg cgcgttcgac ggatccatcg ttgtggtacg
     3121 caccgagaag ccgccggatc gtcggcaggt cgtcgaccgt cccgggccgg agttccggct
     3181 cgccgccgat caggtgcctc ccgcggcccg cgggccgcgg ccgtagcggg ccatgacgtc
     3241 cagcaggcgc tcgtggtcga gggcgtgggc cgtgacatcg tcccggccgc tcagcgtctc
     3301 cgcggcgagc agcgcgttca cgatcgcggc ctcggtcgcc tcgacggtcg ccttgaacag
     3361 cggcgtcatc cggttgtcgg agaccatccg cacctcgtgg gtcatcggcg ccgcgtgccc
     3421 cgggtcatcg gtcgtcgaca ggcctcggtt gccggtcgcg aacgcgagga ccaggtcgcc
     3481 cgacgagtgg ttcgcggtgc tgcccatccg cgccaggccg agcgtcgcgc gctgggcgag
     3541 ccgctcgcac tggtggggcc ggagcggggc gtcggtcgcg acgatgacga tgatcgagcc
     3601 cgttcccggc tcgatcgcgg gtgcacggcc accgccggca gcggcgcgca ggtcgtcggc
     3661 cgcctccgcc gcgtcgtacg gcgagggcac gctcgtgtcc gggatctcga tgcccacggg
     3721 caccccgtcg acgcgaagct gctcccgttc gccgtagttg gcctggacga gggcgccgac
     3781 ggtccagccg ccgtcggcct cggcgaccac acgggacgcg ctcccgatgc cgcccttgaa
     3841 ctcgtggcac accatgcccg tcccgccacc gacgttgccc tgggcgacgg gtccaccggt
     3901 cgcgctcgca atcgccgcgt cgacgtgctc ggggcggacg tggaggccgt cgatgtcgtt
     3961 gagcagcccg tcccacgtct cgccgacgac cggcagcgac cacaggccgc ccccgctgcg
     4021 ccgatgcgcg atggcgtggg cgaccagcgc gtcgcgcacg acgcccacgc tgtgggtgtt
     4081 ggtgatcgcg atcgggccgc cgagcaggcc cgcctcgcgg atccactcga gtccggtcag
     4141 ctcgccgttg ccgttgagcc gatgacagcc cgagaacacg ggctccgccc agacgtcgtc
     4201 gccgtggggc aggatcacgg tgacgccggt gcgcacgggg ccgcggccga cgaccatcgg
     4261 gccatggcca tcgatcagcg tcgtgtggcc cacccgcacg ccgcgcacat ccgtgatcgc
     4321 gtcgttgggg ccggcttcga ggcggccgat gcggatcccg agctgccgag cgggcacgtt
     4381 ccctcccatg acggtgcgtc gccggtgtgc agacggcccc catatcatcg ctgcgtgttc
     4441 aacccaccga gaggccattc cgcgcagcga gccgccggcg ctccctcgag cgtcaggttg
     4501 cccacgctcg acggcatcgc gccgatggac gcggcggaag cgttccgcga cctccccggc
     4561 ctcgccctgc tcgaaagcgc gcgtcctggg cgaaccggcc gctggagcta cctgaccgcc
     4621 gatccggccg cggtggtcga cgcacccgcc gacggcgccg acccatttgc cgaggcacgg
     4681 tcgatgctgg cgcggttgga cgggggcggt ggcggcgatt cacccgggcc cccgttcgcc
     4741 ggcgggctcg tcgggtacct gggctacgac ctcggccggc gcttcgagcg gctgcccgag
     4801 atcgcgcgcg tggaccagca cctgccgatg ctccggctcg cgctccacga ttgggtgctc
     4861 gcctgggacc ggcgcaccgg ggtggcctgg ctggcggcgc gggcggtcga cggtgatgtt
     4921 gcgagcctgc ggcggcggat ggacgagatc caggctcgcg tgcgggcgtc ggtggcgcga
     4981 gaggagccct cggcgccatc gatcccgtcg acgttcgtct cgctcaccca gcacgacgcc
     5041 tgggtccgcg acgtggaggc cgtccgctcc gccatcggcc gcggcgacat ctaccaggcg
     5101 aacctgaccc gccgcctcga ggctccgttc gaggacgatc cttggcccgt cttccgccgc
     5161 ctccggaccg gcgacccggc cctgttcgcc ggcttcctcg acatcggcca ctcgcccgcc
     5221 aacggccggc cccgcgccct gctgtcggcc tcacccgagc cgttcctctc ggtggatgcc
     5281 cagggcgtcg tgtccacgga cccgatcaag ggcacacgcc cgcgcggccg gacccgggag
     5341 caggaccggg cgctcgcccg cgagctgctc gcgagcccca aggaccaggc cgagaacgtg
     5401 atgatcgtgg acgtgctgcg caatgacctc ggccgggtct gcgagcccgg ctcggtgcgc
     5461 gtgccccggc tgctgcgcct cgagcgcacc gccgccgtgc agcacctggt cacgacggtt
     5521 accggccagc tgcgccccga cgcagacgcg ttcgacctgc tcgcgtcggc ctttcccggc
     5581 ggcagcatca ccggcgcccc gaagatccgg gcgatggagc tcatcgagcg ccttgagccg
     5641 gtccgccgcg gcccctactg cgggacgatg ctgtggctcg ggccggacgg gcggatgggc
     5701 tcgagcatcc tgattcgcac gttcgtcgcg gacggcgagc ggttgaccct ccacgtgggt
     5761 ggcgggatca cgtggcgcag cgaccccgag gccgagtggg acgagacggt cgcgaaggcg
     5821 cgcggcccgc tgtcgtcggt cggggcggtc gaggtcccag ggtgagtggc ccggatccgc
     5881 gtcacgtgtg ggtcgacggc gcgctcctgc cggccgatgg accgcacctg tccgcgttcg
     5941 accgcgcgtt tcagttgggc gacggcatct tcgagacgct ccgggcgcgc ggcggccgcc
     6001 tgaccgaact gaccgagcac actgcccggc tgcagcgctc ggccgacggt ttggggatcg
     6061 agttgccgga cgacgtggcg gagttgctcg agcgagcgat ccgggagctg cttgcggccg
     6121 aagggctcga tggcgccgat ggcgacgcct cggtgcgggt cacggtctcg cgcggcgcgt
     6181 ggatgtctcg cggcctcctg ccgccgcgcg acgaacgcct cacggcgacg atcgtgatcc
     6241 aggcgtggcc ggtcctcgcg ccgcccgccg accatctgtc gcgcgggttg tcgatcgtgg
     6301 catcgagcgt gcgccgcgac ccgcagaacc cgctggcggc cctcaagacc acgagccgcg
     6361 ctgactacgt cttcgcccgc ctcgaggccc gtcgtgccgg cggtgacgac gcgctgttcc
     6421 tcacgatctc cggccacctc tcggaggcca cgacggccaa catcttcctg gttcggacat
     6481 cggccgaagg ggtggtcgag ctcgcgaccc cggcgctcga ctgcgcgatc ctcgccggca
     6541 caacgcgctc gtggctgctg cgctggggga gccaggtcgg tcttcggccc gtcgagacct
     6601 ggctgacgcc cgaggacctg gctgcagccg acgaggcctt cgtgtgctcg tcggtggccg
     6661 gcgtgctgcc cgtgacgcgc ttcgacggcg cgccgatcgg cgacggtg
//
LOCUS       DBNHXO010000117         2739 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1615531, whole genome shotgun sequence.
ACCESSION   DBNHXO010000117 DBNHXO010000000
VERSION     DBNHXO010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1615531"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1498
                     /locus_tag="AC162A_02450"
     CDS             83..1498
                     /locus_tag="AC162A_02450"
                     /inference="COORDINATES: protein motif:HMM:NF025112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0052929 - ATP:3'-cytidine-cytidine-tRNA
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR5009725.1"
                     /translation="MAGPDVGAAADRLASAVPAAVVAVLDTLRSHGHAAYLVGGSLRD
                     ALLDREPADWDLATDALPPAIAALFPGAVYENRFGTVAVRREHEVFEITTFRVEHDYA
                     DHRRPHRVEFGGDLVADLSRRDFTVNAIAWGGPADEAGDDDGGRPNTLVDPFDGLGDL
                     AARRLRAVGDPAARFREDALRMVRAVRLAATLGMTVEPATLDAIAGQASLAEHLSGER
                     VGAELSRLLLAPAPSVGLRLAADTGLLAVVVPELAAQPGLAQNKVAGEDLWDHTLRTV
                     DAAPPSRPVVRLAALLHDVGKPDTLADGRFHHHDAVGARLTEVILRRLRYARPTIDTV
                     AHLVRHHMFTVDPDATDVAVRRFIRRIGREHIDDLFELRRADDIGSGLAPDDPATTAF
                     RARVEAQIAARPPLDRSALAVDGDDLIRDLGLEPGPSLGRVLDALLDQVIDDPGLNDR
                     ATLMLLAQGMLADMPRNEVDA"
     gene            1495..1863
                     /locus_tag="AC162A_02455"
     CDS             1495..1863
                     /locus_tag="AC162A_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009726.1"
                     /translation="MIELLLQAERALSVGLVDQADRLYRQVAAADPRNSIAVVGLARV
                     ALERADDAEAWRQAQRALAIDPENVAARRLGDRLEEVFAARGQALPEPPDVDNGAVAA
                     SPTSSASAGLVDRLLRRSRP"
     gene            1860..>2739
                     /gene="galE"
                     /locus_tag="AC162A_02460"
     CDS             1860..>2739
                     /gene="galE"
                     /locus_tag="AC162A_02460"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR5009727.1"
                     /translation="MKVLVTGGAGYVGGVSVDAILDAGHAVVVLDDLTTGHRAIVNPA
                     ARLVVGSYGDPATIAALLEAERPDAILHCAARSLVGESVRDPGRYYRDNVAGGVALLE
                     AARAAGVGGVVFSSTAAVYGVPDTTPIEEDAPLRPINPYGETKRTFEGALRWYGEAYG
                     LRSVSLRYFNVAGATERVGEVHLPETHLVPNALIAAEGGPQLTVFGENYPTPDGTPIR
                     DYIHVADLADAHLSALLLLVADGGRADDEAGDRPPQLVCNLGTSTGFSVREVLGVAGT
                     IVGRPIPHAVGPRRAGD"
BASE COUNT          346 a         1031 c          992 g          370 t
ORIGIN      
        1 tcgctgttca agccgtacct cgaagacgtt cggaacgaag cctggaggac gtacctcttc
       61 ctcggccgga gctgagccgc cggtggcagg cccggacgtc ggagcggcgg cggaccgcct
      121 cgcgtccgcc gtcccggcag ccgtggtcgc cgtcctcgac acgctgcggt cgcacgggca
      181 cgcggcgtac ctcgtcggtg gctcgctgcg cgacgccctc ctggaccggg aacccgccga
      241 ctgggatctc gcgaccgacg ccctcccgcc cgcgatcgcc gccctgttcc ccggggcggt
      301 gtacgagaac cgtttcggga cggtcgccgt gcggcgcgag cacgaggtgt tcgagatcac
      361 gacattccgc gtcgagcacg actacgccga ccaccggcgg ccacaccggg tcgagttcgg
      421 cggcgacctc gtggctgacc tgtcgcgccg tgacttcacg gtcaatgcga tcgcgtgggg
      481 tggacccgcg gacgaggccg gcgacgacga cggtggacgc cccaacacgt tggtcgaccc
      541 gttcgacggc ctcggcgacc tcgcggcccg gcggctgcgg gcggtcggtg atcccgccgc
      601 ccggttccgc gaggacgcgc tgcggatggt gcgggccgta cggctcgcgg ccacgctcgg
      661 gatgaccgtc gaaccggcga cgctcgacgc gatcgcgggc caggcatcgc tcgccgagca
      721 cctctccggt gagcgggtcg gcgctgagct ctcgcggctg ctcctcgcac cggcgccgtc
      781 cgtcgggctg cgcctcgccg ccgacaccgg gctgctcgcg gtcgttgtcc cggagctggc
      841 ggcccagccg ggtcttgcgc agaacaaggt cgcaggcgag gacctgtggg accacacgct
      901 gcggaccgtc gacgcggccc cgccgagccg gccggtggtc cgcctcgcgg cgctgctgca
      961 cgacgtcggc aagccggaca cgctcgccga cggacggttc caccatcacg atgccgtcgg
     1021 tgcccgccta acggaggtga tcctgcgccg gctccggtat gcgcggccga cgatcgatac
     1081 ggtcgcgcat ctcgtccgcc accacatgtt caccgtggac cccgacgcga ccgatgtcgc
     1141 cgtccggcgg ttcatccggc gcatcggccg cgagcacatc gacgacctgt tcgagctgcg
     1201 acgggcggac gacatcggca gcgggctggc accggacgac ccggcgacga cggccttccg
     1261 ggcccgcgtc gaggcccaga tcgccgcgcg gcccccgctc gaccggagcg ccctcgcggt
     1321 cgacggcgac gacctcatcc gggacctcgg gctcgagccg ggtccaagcc tcggccgcgt
     1381 gctggacgcg ctgctcgacc aggtgatcga tgaccccggc ctcaacgatc gcgcgacgct
     1441 gatgctgctg gcacagggca tgcttgccga catgccgcgc aacgaggtcg acgcgtgatc
     1501 gagctgctgc tgcaggccga gcgcgcgctg tcggtcgggc tcgtcgacca ggccgatcgc
     1561 ctgtaccggc aggtcgccgc cgcggacccg cgcaactcga tcgccgtcgt cgggctggcg
     1621 cgtgtcgcgc tcgagcgtgc ggacgatgcc gaggcgtggc gccaggccca gcgagcgctc
     1681 gccatcgatc ccgagaacgt ggcggcgcgc cgcctcggcg accgcctcga ggaggtgttc
     1741 gccgcgcggg ggcaggccct gccggagccg cctgacgtgg acaatggagc cgtcgccgct
     1801 tcaccgacct cgtcggcgtc ggcgggcctc gtcgaccgcc tgctccggag gagccgccca
     1861 tgaaggtcct ggtcaccggt ggggcgggct acgtgggcgg cgtctccgtg gacgcgatcc
     1921 tggacgccgg gcacgcggtc gtcgtcctcg acgacctgac gacgggccat cgggcgatcg
     1981 tcaacccggc ggcacgcctg gtcgtcggct cgtacggcga cccggccacg atcgccgcgc
     2041 tcctcgaggc cgagcgcccg gacgcgatcc tccactgcgc ggcgcggtcg ctcgtcggcg
     2101 agagcgtgcg cgaccccggc cgttactacc gggacaacgt cgcgggcggt gtcgcgctgc
     2161 tcgaggccgc gcgcgccgcc ggcgtcggcg gggtcgtctt ctcctcgacc gcggccgtgt
     2221 atggcgtccc cgacacgacg cccatcgagg aggacgcgcc gctgcggcca atcaacccgt
     2281 acggcgagac caagcgcacg ttcgagggcg cgctccgctg gtatggggag gcgtatggcc
     2341 tgcgcagcgt gagcctccgc tacttcaacg tcgccggcgc caccgagcgg gtcggggagg
     2401 tccacctgcc cgagacgcac cttgtgccca acgcgctgat cgccgcggag ggcggcccgc
     2461 agctgacggt gttcggcgag aactacccga cgcccgacgg gacgccgatc cgcgactaca
     2521 tccacgtcgc cgacctggcg gacgcccatc tctcggccct gctgctgctg gtggcggacg
     2581 gcggccgggc cgacgacgag gcgggtgaca gaccgccgca gctcgtctgc aatctcggca
     2641 catcgaccgg cttctcggtc cgcgaggtcc tgggcgtcgc cgggaccatc gtcggccgcc
     2701 cgatcccgca cgccgtgggt ccgcgccgcg ccggcgatc
//
LOCUS       DBNHXO010000118         1749 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2409365, whole genome shotgun sequence.
ACCESSION   DBNHXO010000118 DBNHXO010000000
VERSION     DBNHXO010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2409365"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="AC162A_02465"
     CDS             complement(<1..713)
                     /locus_tag="AC162A_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR5009728.1"
                     /translation="MTATASDADRLDRAIEDYLTYLRVERGVADATIRAYRGDLADFA
                     SSRGAAARWSAGPEVAQGYLAARARRGRPNDPGLAPTSLRRRAAAIRGFYRFAFGDGL
                     IDVDVAAHLELPRQPRLLPETLTVDEVERLLEAAGGDPGAEPTATDALRVRNRALLEV
                     LYAAGLRVSEALGLDLGAVRLESAWVRVIGKGDRERVVPVGDVAMDWLARYLAWPRAE
                     WLAAAGVPDGPTTPLFIAPR"
     gene            complement(716..>1749)
                     /locus_tag="AC162A_02470"
     CDS             complement(716..>1749)
                     /locus_tag="AC162A_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR5009729.1"
                     /translation="GGGAREQVARAERELRSLARLDPRWGPLADRLAGLEAEIEDVAA
                     EARTLAEAVDHDPTTIARLEERLGEIHRLLRRYGDDEATVIEHGERSAAEAERLRGLE
                     DERARRAADDARLLLEVADAAASLSIRRAETATGLAASVGAVLVELGFPADAFDVALG
                     RRPAARDDPAVEVDGDAVAFDVTGIDTVVFTFRPNAGEPARPLARIASGGELSRVALA
                     VKQVLAEVDDTPTLVFDEIDTGIGGRSADPVARSLWALARDHQVLCVTHLPQIAAYAD
                     AHYRIAKRERDGRTVTDVTRLERDEREDELAQMIGGTAGGDAARLSARELLDRAEAWR
                     VGRLADAPV"
BASE COUNT          207 a          704 c          632 g          206 t
ORIGIN      
        1 cgcggggcga tgaacagcgg cgtcgtcggg ccgtcgggga cgcccgcggc ggcgagccac
       61 tccgcgcgcg gccaggcgag gtaccgggcc agccagtcca tcgcgacgtc gccgaccggc
      121 acgacacgct cgcggtcgcc cttcccgatc acgcgcaccc acgccgactc gagcctgacc
      181 gcgcccagat ccagtccgag cgcctccgac acgcgcagcc ccgcggcgta gaggacctcg
      241 agcagcgccc ggttgcggac gcgcagcgcg tcggtcgccg tcggctccgc ccccgggtcc
      301 ccgcccgccg cctcgagcag ccgctcgacc tcgtccaccg tgagggtctc gggcagcagg
      361 cgtggctggc ggggcagctc gaggtgcgcc gcgacgtcga cgtcgatcag gccatcgccg
      421 aacgcgaacc ggtagaagcc gcggatcgca gcggcgcgcc ggcggaggct cgtcggggcg
      481 agccccgggt cgttgggccg gccacggcgg gcgcgggcgg cgaggtaccc ctgcgcgacc
      541 tccggcccgg ccgaccagcg cgccgccgcg ccgcgactcg aggcgaagtc ggcgagatcc
      601 ccgcgatagg cccggatcgt cgcgtcggcg acgccacgct cgaccctcag gtacgtgagg
      661 tagtcctcga tcgcccggtc gaggcggtcg gcgtcggacg cggtcgccgt cacgctcaga
      721 cgggcgcgtc ggccaggcgg ccaacgcgcc acgcctcggc acgatcgagc agctcgcgcg
      781 ccgacagccg cgccgcgtcg ccgcccgcgg tgccgccgat catctgggcg agctcatcct
      841 cgcgctcgtc gcgctccagc cgggtgacgt cggtcacggt gcggccgtcg cgctcccgct
      901 tggcgatccg gtagtgggcg tcggcgtagg cggcgatctg gggcaggtgg gtcacgcaca
      961 gcacctggtg gtcgcgcgcc agcgcccaca ggctgcgcgc taccgggtct gcgctgcgac
     1021 cgccgattcc cgtgtcgatc tcgtcgaaca cgagcgtcgg cgtgtcgtcg acctcggcca
     1081 gcacctgctt cacggccagg gcgacccgcg acagctcgcc gcccgacgcg atgcgcgcca
     1141 gcggtctcgc cggttcgccc gcgttgggtc ggaacgtgaa cacaacggtg tcgatgccgg
     1201 tgacgtcgaa cgcgacggca tccccgtcaa cctcgaccgc cgggtcatca cgcgcggccg
     1261 gccgccggcc gagggcgacg tcgaaggcgt cggccgggaa gccgagctcg accagcaccg
     1321 cgccgaccga ggccgcgagc ccggtcgccg tctccgcccg ccggatcgag aggctcgccg
     1381 ccgcgtcggc gacctcgagc aggagccgcg cgtcgtcggc cgcccgcctc gcccgctcgt
     1441 cctcgagccc ccgcagtcgc tcggcctccg ccgccgatcg ttcgccgtgc tcgatgacgg
     1501 tcgcctcgtc gtcgccgtac cgccgcagga gccgatggat ctcgcccagg cgctcctcga
     1561 gccgcgcgat cgtggtcggg tcgtggtcga ccgcctcggc cagcgtgcgg gcctccgccg
     1621 cgacatcctc gatctccgcc tcgagaccgg cgaggcgatc cgccagcggc ccccagcgcg
     1681 ggtccagccg ggcgagcgac cgcagctcgc gctcggccct cgccacctgc tcgcgggccc
     1741 cgccgccct
//
LOCUS       DBNHXO010000119         2825 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_822077, whole genome shotgun sequence.
ACCESSION   DBNHXO010000119 DBNHXO010000000
VERSION     DBNHXO010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_822077"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..640
                     /locus_tag="AC162A_02475"
     CDS             227..640
                     /locus_tag="AC162A_02475"
                     /inference="COORDINATES: protein motif:HMM:NF025066.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HIR5009730.1"
                     /translation="MTEPLNLVLFSGTDDKLQAASVLAAGAAALGKPVNIFLQYWALD
                     AFRSDRIARDHGLAPEAGEAGRAAVAALARAGQAPWAATLRDAKDLGEVDIQACSLSM
                     DLLALEPADLDPLVDGVEGVTAFYLNAGDGQVVFI"
     gene            666..902
                     /locus_tag="AC162A_02480"
     CDS             666..902
                     /locus_tag="AC162A_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR5009731.1"
                     /translation="MPVIEITTWVDARGLSCPMPIVKCAQAIKGIPSGAAIELLATDH
                     GSPKDVAAWCRATGNELVDQSSDGAVYRFVIRRK"
     gene            910..1503
                     /locus_tag="AC162A_02485"
     CDS             910..1503
                     /locus_tag="AC162A_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HIR5009732.1"
                     /translation="MTTELLVPIDPLEAAIAAVENLEEPVPLPAAELSTQGDVRVKDL
                     LIIDYSGDLEKVWATLILASTSAAMGVRTRVFVTFWGLQVFVKDSKRITGQNWMQRML
                     SAMQRPGISHRKLSKMNFLGMGPWMMGRLAKQYGVASPKELLEAAQAMGVEFIPCQMT
                     MDMFGLKPEDMIEGMGEPAGAATVIGLLTGDSTPLFI"
     gene            1530..1766
                     /locus_tag="AC162A_02490"
     CDS             1530..1766
                     /locus_tag="AC162A_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010901577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR5009733.1"
                     /translation="MATTPTRIVDARGSFCPGPLMELIRAIRESAVGDVIAVYSSDTG
                     SKTDIPKWVEKAGHRLVALDARDGYDEIVVEKRR"
     gene            1777..>2825
                     /locus_tag="AC162A_02495"
     CDS             1777..>2825
                     /locus_tag="AC162A_02495"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5009734.1"
                     /translation="MQHVLILGGGVGGTLTANLLARQLGDRIDRGEVIVTVVDATGDH
                     VYQPGFMYIALGGERAEKLKRPERSLLDKRVHLQVGEVTALDAERRIVTLADGRVLAY
                     DQLVLATGSRIVPEAIEHFDTQAQHFYSAEAALELRAALDRFTGGRIVIGIAGMPYKC
                     PPAPLEVAFLIEAELRERGLRERTTIDYCSPIGRAFTIESVSEMATPILAQKGIELHT
                     FFNVEAIDAERKVVLSLEGEELPYDLLILVPPHKGQQFLVDAGLAPAPGGWLPTDRAT
                     LQVGGRADVYALGDATDLPLSKAGSTAHFEAPVVAERIAAAIEGRAIDPGRGWYQGKV
                     MCFFEIGDGKGTLLR"
BASE COUNT          430 a          997 c          974 g          424 t
ORIGIN      
        1 tcctcgtgcg ggtctgtccg ggtcctacgg tcgatccgcc ggcgacgcgg taccagtgcc
       61 gcccggcccg tcgtgccggt acccgacggc ccggatcctg aatgcgacgg gacttccagc
      121 ccgtcgcagg ggggtcgggc ggcacttatc ccgcacggtc gcgcgggcgg aacgtggcgt
      181 cgatgaccga ggctccgtgc ccggccgagc acctggggac gccaccatga ccgagccgct
      241 caacctggtc ctgttctcgg gcaccgacga caagctccag gccgcgtcgg tcctggcggc
      301 cggcgccgcc gccctgggca agcccgtcaa catcttcctc cagtactggg cgctcgacgc
      361 cttccgctcg gaccgcatcg cccgtgacca cgggctcgcc cccgaggccg gcgaagccgg
      421 ccgcgccgcc gtcgccgcgc tcgcacgcgc gggtcaggcc ccgtgggccg cgacccttcg
      481 cgacgccaag gacctgggcg aggtcgacat ccaggcctgc tcgctgtcga tggacctgct
      541 cgcgctcgaa cccgccgatc tcgacccgct cgttgacggg gtcgaaggcg tgaccgcgtt
      601 ctacctcaac gccggcgacg gacaggtcgt cttcatctga tcccgatcac ccggaggaac
      661 ccgccatgcc agtcatcgag atcacgacct gggtcgacgc tcgcgggctg tcgtgcccca
      721 tgccgatcgt gaagtgcgcc caggcgatca aggggatccc gagcggcgcc gcgatcgagc
      781 tcctcgcgac cgatcacgga tcgcccaagg acgtcgcggc gtggtgtcgg gcgacgggca
      841 acgagctcgt cgaccagtcg tcggacggcg ccgtctaccg cttcgtcatc cgcaggaagt
      901 gagcacccca tgaccaccga gttgctggtc ccgatcgacc cgctggaggc ggcgatcgcc
      961 gccgtcgaaa acctcgagga gcccgtcccg ctgcccgcgg ccgagctgtc gacccagggc
     1021 gacgtgcggg tcaaggacct cctgatcatc gactacagcg gcgacctcga gaaggtctgg
     1081 gccacgctga tcctggcctc cacgagcgcc gcgatgggtg tccggacccg cgtgttcgtc
     1141 acgttctggg ggctccaggt cttcgtcaag gactcgaagc ggatcaccgg ccagaactgg
     1201 atgcagcgga tgctgtccgc gatgcagcgg cccgggatca gccatcgcaa gctgtcgaag
     1261 atgaacttcc tgggcatggg gccctggatg atgggtcgcc tggcgaagca gtacggcgtt
     1321 gccagcccca aggagctcct cgaggccgcc caggcgatgg gcgtcgagtt catcccatgc
     1381 cagatgacca tggacatgtt cggcctcaag ccggaggaca tgatcgaggg catgggcgag
     1441 cccgccgggg ccgcgacggt catcggcctg ctgaccgggg actccacccc gctgttcatc
     1501 tagcggtaca ggtgaccacc atgaccgaga tcgcaaccac cccgacgcgg atcgtcgatg
     1561 cccgcgggag cttctgcccc ggcccgctga tggagctcat ccgggccatc cgcgagtccg
     1621 ccgtcggcga cgtgatcgcc gtgtactcct cggacacggg ctcgaagacc gacatcccca
     1681 agtgggtcga gaaggccggc caccggctcg tcgcgctcga cgcccgggac ggctacgacg
     1741 agatcgtcgt cgagaagcgg cgctgaggtg gccgccatgc agcatgtcct gatcctcggg
     1801 ggcggggttg gcgggaccct gaccgccaat ctcctggccc gccagctggg cgatcggatc
     1861 gaccgtggcg aggtcatcgt cacggtcgtc gatgccacgg gcgatcacgt ctaccagccg
     1921 gggttcatgt acatcgcgct gggcggtgag cgggccgaga agctcaagcg accggaacgc
     1981 tcgctgctcg acaagcgcgt gcatctccag gtcggcgaag tgaccgcgct cgacgcggag
     2041 cgccggatcg tcacgctcgc ggacgggcgg gtcctcgcct acgaccagct cgtgctggcg
     2101 accggctcgc gcatcgtccc cgaggccatc gagcacttcg acacgcaggc gcagcacttc
     2161 tactcggcgg aggccgcgct ggagctgcgg gccgccctcg accggttcac cggcgggcgg
     2221 atcgtcatcg gcatcgccgg gatgccctac aagtgcccgc cggcgccgct ggaggtcgcg
     2281 ttcctcatcg aggcggagct ccgtgagcgc gggctccgcg aacggaccac gatcgattac
     2341 tgctcgccga tcgggcgcgc gttcacgatc gagagcgtgt ccgagatggc gacgccgatc
     2401 ctcgcccaga aggggatcga gctgcatacg ttcttcaacg tcgaagcgat cgacgccgag
     2461 cgcaaggtcg tcctgagcct ggagggcgag gagctgccgt acgacctgct catcctcgtg
     2521 ccgccgcaca agggccagca gttcctggtc gatgcgggtc tggcgccggc cccgggcggc
     2581 tggctgccga ccgaccgcgc cacgctccag gtcggtggtc gcgcggacgt ctacgccctg
     2641 ggtgacgcca cggatctgcc gctgtccaag gcgggctcca cggcgcactt cgaagccccc
     2701 gtggtcgccg agcggatcgc ggcggcgatc gagggacgcg ccatcgatcc gggtcgtggc
     2761 tggtaccagg ggaaggtcat gtgcttcttc gagatcggcg acggcaaggg caccctgctg
     2821 cggtt
//
LOCUS       DBNHXO010000120         4744 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3600137, whole genome shotgun sequence.
ACCESSION   DBNHXO010000120 DBNHXO010000000
VERSION     DBNHXO010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3600137"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..2681
                     /locus_tag="AC162A_02500"
     CDS             228..2681
                     /locus_tag="AC162A_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcvT family protein"
                     /protein_id="HIR5009735.1"
                     /translation="MVAHARAVIIGGGVGGTSIAYHLAALGWTDVVLLDRAEMTSGST
                     FHSAGLVGQLRSTVTLTRMMMYGVDLYRRLADETGVDPSWHEVGSLRLASSPARFEEL
                     QRQAGWAASFGLPIELISAAEARERFPLMTTDGVLGAVWLPTDGWLDPSNLAFALAAG
                     ARKRGVTIRNHHRVVAIGVRDGRVTGVDVEHDGEREMIEAEVVVNAGGMYAPDIGRLA
                     GITLPIIPMAHQYLLTAPIDGVEPGLPQLRDPDNLVYFREEVGGLCMGGYERNPMPWA
                     LDGVPADFNHRLLDPDWPRFEEIMAGAIRRVPAIADAPVTRMINGPEGFTPDNEFILG
                     ESEVRGLFVAAGFCAHGIAGAGGIGRQVATWIVEGEPELDLWKMDIRRFGAQYRSRSY
                     TLARSYENYATYYDIHYPNEERQAGRPLRVSPAYSRLAALGAVFGEKSSWERANWFEP
                     NGGGAGVADAAALGALRPRGWAGEHWSPAIGAEALATRTTAALFDETSFAKIEIVGSG
                     AAGLLQWLCANDVDREVGSITYTQLLNRRGGIECDLTVTRVAADRFLLVTGTAFGNHD
                     LAWIRHHAPSDGSVMVDDVTSARVCYALWGPRARDILAATTTDDVADAAFPYLTARTI
                     TVGSVPVYALRVTYVGELGWELYAPAEYGGALWDTLWEAGMAHGLVAGGYRAIDALRL
                     EKGYRAWAADITPEDTPYEAGLGFAVSLEKGDFLGRDALVAAKAAGPRKRLRCLILDD
                     PRSVCLGNEPVRIDGRIVGRVTSGGYGFSVERSLAYGYLPPDVAIGTRGEVDLFGTWV
                     GFEVAREPLFDPSGSRIRA"
     gene            2701..3552
                     /locus_tag="AC162A_02505"
     CDS             2701..3552
                     /locus_tag="AC162A_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR5009736.1"
                     /translation="MTNASSDPGFGPEWSAGRARVPESQLREWLDFALAACDAADEVA
                     RRHFRRDLDLERKPDRSFVTVADRAIELEIRDGILARWPDHGLVGEEYGEDAGRSTTR
                     WYIDPIDGTHNFIRGVPLFGTLLAVEHEGEMQVGVISAPAMRERWYAYRGGGAWSDGQ
                     DGRRQVRVSRVAALEDAQLVYGSLRDNVDSGLMPGFTDLIDASWRDRGFGDFWGYALV
                     AEGAAEAMMETGMHTWDLAAPQVVIEEAGGRVTDVNGERRIDAESFVGSNGLLHDEIL
                     RRLRAAG"
     gene            complement(3562..3858)
                     /locus_tag="AC162A_02510"
     CDS             complement(3562..3858)
                     /locus_tag="AC162A_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009737.1"
                     /translation="MTESNQPTEVESPPAPGPTPITATTSFEDQERAVGIDPDAPLPG
                     EGGLRIILFGAIALFGIPAVLGGGIAAGYPIVGIVLAVALGAVCLLFARPRQRA"
     gene            complement(3887..>4744)
                     /locus_tag="AC162A_02515"
     CDS             complement(3887..>4744)
                     /locus_tag="AC162A_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR5009738.1"
                     /translation="KDVYRGAFEPLPGSVYHAAYPYCYRATGGSHDPSACTCDWEDQL
                     DLLFHQLVYPDKVAGIIVEPVIGEGGYIVPSPTFLPRLREIASKHGILLIADEVQTGF
                     GRTGELFAVDHWNVEPDILVMAKGIASGLPLSGIIARKELMDRLPPGSHGGTYGGNVV
                     SCAAANATLDVIEDEGLVANARERGRQLLDGLRRACAGRVSVGDVRGLGLMVAVEFVK
                     PGEGDGRVPNPELAKRVQHEAFARNLLMLTAGTYVNVIRIIPPLVTTEDEVDRAIGIL
                     SESLDAAGA"
BASE COUNT          658 a         1642 c         1756 g          688 t
ORIGIN      
        1 gcgagacgca gcccaactcc gtcgtccgaa aggcccggct gctgggcgtg ttccaggtca
       61 cattgcaccc tgacggctgg tcgtaccgct tcatctccac ggacggatcg ttctccgacg
      121 agggaacggc ggactgccac tgatcgcccg atgggacgtc ccggcggcgc gggagtgccg
      181 ccgctaggct tcccgtcagc aacgggagca ggagggttgg cgaggcggtg gtcgcgcacg
      241 cacgggcggt gatcatcggc ggcggcgtcg gggggacgtc gatcgcgtac cacctcgccg
      301 cgctcggctg gaccgacgtc gtgctgctcg accgggcgga gatgacgtcc ggctcgacgt
      361 tccacagcgc tgggctcgtc gggcagctgc gcagcaccgt gacgctgacg cgaatgatga
      421 tgtacggcgt cgacctgtat cgccggctgg cggacgagac cggggtcgat ccgtcgtggc
      481 acgaggtcgg gtcgctccgc ctcgcgtcga gcccggcccg cttcgaggag ctccagcgcc
      541 aggcgggctg ggccgccagc ttcgggctgc cgatcgagct gatctcggcg gcggaggcac
      601 gcgagcggtt cccgctgatg acgacggacg gtgtgctggg tgcggtctgg ctgccgaccg
      661 acggctggct cgacccgtcg aacctggcgt tcgcgctggc ggcgggggct cggaagcggg
      721 gggtgacgat ccgcaaccat catcgagtgg tggcgatcgg cgtgcgcgac gggcgggtca
      781 cgggcgtcga cgtcgagcac gacggcgagc gcgagatgat cgaggccgag gtcgtggtca
      841 acgccggcgg catgtacgcc cccgacatcg gccgcctggc cgggatcacg ctgccgatca
      901 tcccgatggc gcaccagtac ctgctgacgg ctccgatcga cggcgtcgag cccggcctgc
      961 cccagctgcg cgacccggac aacctcgtgt acttccgcga ggaggtcggc ggcctgtgca
     1021 tgggcggcta cgagcgcaac ccgatgccct gggccctcga cggcgtcccg gccgacttca
     1081 accaccgcct gctcgacccc gactggccgc ggttcgagga gatcatggcc ggcgccattc
     1141 gccgggtccc cgcgatcgcc gacgcgcccg tgacgaggat gatcaacggc cccgaggggt
     1201 tcacgcccga caacgagttc atcctgggcg agtcggaggt gcgcggcctg ttcgtggccg
     1261 ccgggttctg cgcccacggg atcgccgggg cgggcggcat cgggcgccag gtggcgacct
     1321 ggatcgtcga gggcgaaccc gagctcgacc tgtggaagat ggacatccgc cgcttcggcg
     1381 cccagtaccg gagccgctcg tacacgctcg cgcggagcta cgagaactac gcgacctact
     1441 acgacatcca ctacccgaac gaagaacggc aggccgggcg gccactccgc gtctcgccgg
     1501 cctactcgcg cctcgcggcc ctcggcgcgg tgttcgggga gaagtcgtcg tgggagcgcg
     1561 ccaactggtt cgagccgaac gggggcggcg cgggcgtggc ggacgcggcc gcgctcgggg
     1621 cgcttcgccc gcgcggctgg gccggcgagc actggagtcc cgcgatcggg gccgaggcgc
     1681 tcgcgacgcg gacgaccgcc gccctgttcg acgagacgtc gttcgccaag atcgagatcg
     1741 tgggttcggg cgcggccggg ctgctgcagt ggctgtgcgc gaacgacgtc gaccgcgagg
     1801 tcggcagcat cacctacacc cagctgctca accgacgcgg cgggatcgag tgcgacctca
     1861 cggtgactcg cgtcgccgct gaccgcttcc tgctcgtgac cgggaccgcg ttcgggaacc
     1921 acgatctcgc ctggatccgc caccacgcgc cgagcgacgg ctccgtgatg gtcgacgacg
     1981 tgacctcggc ccgggtctgc tatgccctgt gggggccgcg cgcccgcgac atcctggccg
     2041 cgacgacgac cgacgacgtc gccgatgccg ccttcccgta cttgaccgcc cggacgatca
     2101 cggtcgggtc agttcccgtg tacgcgctcc gggtgacgta tgtcggcgag ctcggctggg
     2161 agctgtatgc gccagccgag tacggcggtg cgctgtggga cacgctgtgg gaggcgggca
     2221 tggcgcacgg cctcgtcgcc ggcggctatc gcgccatcga cgccctgcgg ctcgagaagg
     2281 gctatcgggc gtgggcggcc gacatcacgc ccgaggatac gccgtacgag gccggcctcg
     2341 gttttgcggt gagcctcgag aagggcgact tcctggggcg agacgcgctg gtggcggcga
     2401 aggcggccgg accgcggaag cgcctgcggt gcctcatcct cgacgatccg cgttcggtct
     2461 gcctcggcaa tgagcctgtg cgcatcgacg gccggatcgt gggcagggtg acctcgggcg
     2521 ggtacgggtt cagcgtcgag cgcagcctcg cctacgggta cctgccgccc gacgtcgcga
     2581 tcgggacccg cggcgaggtc gacctgttcg ggacgtgggt cggattcgag gtggcgaggg
     2641 agccgctgtt cgacccctcg ggctcgcgga tccgcgcctg agcagggcat catcggcgcc
     2701 atgacgaacg ccagctccga tcccggcttc gggcccgagt ggtccgccgg ccgcgcccgt
     2761 gtccccgaat cgcagctgcg cgagtggctc gacttcgcct tggccgcctg cgacgctgcg
     2821 gacgaggtcg cccggcggca cttccgccgc gacctcgatc tcgagcgcaa gcccgaccgc
     2881 tcgttcgtga ccgtcgccga ccgggcgatc gagctggaga tccgggacgg catcctcgcc
     2941 cgatggccgg accacggcct ggtcggcgag gagtacggcg aggacgccgg ccgctcgacg
     3001 acgcgctggt acatcgaccc gatcgacggc acccacaact tcattcgcgg cgtgcccctg
     3061 ttcgggacgc tgctcgcggt ggagcacgag ggcgagatgc aggtcggcgt gatctctgcg
     3121 cccgcgatgc gcgagcggtg gtacgcgtac cggggtggcg gcgcgtggag cgacggccag
     3181 gacggcaggc gccaggtccg cgtgtcgcgg gtggccgcgc tcgaggacgc ccagctcgtc
     3241 tacggcagcc ttcgcgacaa cgtcgatagc gggctcatgc ccgggttcac cgacctgatc
     3301 gacgcctcgt ggcgtgaccg cggtttcggc gacttctggg gctacgcgct ggtcgcagag
     3361 ggcgccgcgg aggcgatgat ggagaccggg atgcacactt gggacctcgc ggcgccccag
     3421 gtcgtgatcg aggaggcggg cggccgggtc acggacgtga acggggagcg gcggatcgac
     3481 gcggagtcat tcgtgggctc caacggcctg ctccacgacg agatcctgcg ccggctgcgc
     3541 gcggctgggt agctccgcgg gtcacgcgcg ctggcggggc ctcgcgaaca gcaggcacac
     3601 cgccccgagc gcgacggcga ggacgatccc gacgatcggg tatccggccg cgatcccgcc
     3661 gcccagcacc gccgggatgc cgaacagggc gatcgcgccg aacaggatga tccgcaggcc
     3721 gccctcgccc ggcagcggtg cgtcggggtc gatcccgacg gcacgctcct ggtcctcgaa
     3781 gctggtggtc gcggtgatcg gggtcggtcc gggcgcgggc ggggactcga cctcggtggg
     3841 ctggttcgac tcggtcacgg ttctcctcgc tgcctggacc gtgtcgtcaa gcgccggctg
     3901 cgtccagtga ctcgctgagg ataccgatcg cccggtcgac ctcgtcctcg gtcgtgacca
     3961 gcggcgggat gatccggatg acattcacgt acgtgccggc cgtgagcatg agcaggttcc
     4021 tcgcgaacgc ctcatgctgc acccgctttg cgagctcggg gttcggcacc cggccgtccc
     4081 cctcgcccgg cttcacgaac tcgaccgcga ccatcagccc caggccgcgg acgtcgccca
     4141 ccgacacccg gcccgcacac gcccggcgca gtccgtcgag cagctgtcgg ccgcgttcgc
     4201 gggcgttcgc gacgagcccc tcgtcctcga tgacatccag cgtggcgttg gcggccgcac
     4261 acgagacgac gttgccaccg tacgtcccgc cgtgcgaacc cggcggcagc cggtccatca
     4321 gctccttgcg ggcgatgatc cccgacagcg gcagcccgga cgcgatgccc ttggccatga
     4381 ccaggatgtc gggctccacg ttccagtggt cgacggcgaa cagctcgccc gtgcgcccga
     4441 accccgtctg gacctcgtcg gcgatgagca ggatcccgtg cttgctggcg atctcgcgca
     4501 ggcgcggcag gaacgtcggt gacgggacga tgtagccgcc ctcgccgatg accggctcga
     4561 cgatgatccc ggccaccttg tcggggtaga cgagctggtg gaacagcagg tcgagctggt
     4621 cctcccagtc gcaggtgcag gccgacgggt cgtgggagcc gccggtggcg cggtagcagt
     4681 acggataggc cgcgtggtag acagaacccg gcagcggctc gaacgcgcca cggtagacgt
     4741 cctt
//
LOCUS       DBNHXO010000121         2589 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4394304, whole genome shotgun sequence.
ACCESSION   DBNHXO010000121 DBNHXO010000000
VERSION     DBNHXO010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4394304"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..801)
                     /locus_tag="AC162A_02520"
     CDS             complement(58..801)
                     /locus_tag="AC162A_02520"
                     /inference="COORDINATES: protein motif:HMM:NF015648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR5009739.1"
                     /translation="MSYADGLLATDETVVRREHQHWIFPFYIAGKWVAVAVLVTVVGF
                     ILSQWVFKSDGTGIIGGTIAFINNVVTIVTVIALAFAVIGFIYSVIQWQSQEYILTTV
                     RVLHVHGVINKQSGDSSLENITDAQIRVPWLGRILGFGDLVVMTASEAGINNLRALRD
                     PIGFKKAMMESKTERMIALNTPRAAAAPAPAPGPVAPAPVVPAPPAAPVPNADDVTKT
                     LASLASLRDSGAITPEEYEAKKQELLDRI"
     gene            complement(924..2198)
                     /locus_tag="AC162A_02525"
     CDS             complement(924..2198)
                     /locus_tag="AC162A_02525"
                     /inference="COORDINATES: protein motif:HMM:NF024241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5009740.1"
                     /translation="MTRRARLWLDAWGPMLPMLLAEASIWVGFGALLPILPIYFTEHG
                     VDLPTLGIVVAAWPAARLIGEPLFGWVADRGPRKAMMIVGLLLAAVFAVAPLFIVGPA
                     AFIVLRALSGLSASIYDPAARGYLVDANPPERQGEMFGLYGAAQMGGLMIGPAIGGIA
                     AGLTGQPTVVFWVAGLMTIVSAIVVAARVPELAHRTPATAGHMAPAAEAAEAPRQPGS
                     IVNRLLIAAVAFNIGSYVASGAYEVIWSLYLTHLGASLGLIGLTFFTFALPILVLSPW
                     FGRFIDREGGFFALVIGMAGVGLCGLLYPLVPEIWWMAALGIVEGTAFALASPALFLL
                     VARASPAGRSSTAQGIFGAAGTVGTIVASLTAGALAAINLTYPFYATGIVALASLALG
                     LAIGRRRLWDAMQPTHLAPAAVPATSTAREAG"
     gene            2295..>2589
                     /locus_tag="AC162A_02530"
     CDS             2295..>2589
                     /locus_tag="AC162A_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009741.1"
                     /translation="MAPLLLLSILVAGLVALLPVWRLHVAGWPVRWLFTAWILYAIGI
                     LAVLRLPGLRFVIPIVVLAYIAPFVAGPERVSRVLSGRRTTPAVVIDVTPRAAP"
BASE COUNT          388 a          887 c          954 g          360 t
ORIGIN      
        1 accccgcggc tatcaacact accggcgatc ggcggttccg tcaaaggccg gcgtggccta
       61 gatgcggtcg aggagctcct gcttcttggc ctcgtactcc tcgggcgtga tggcaccgga
      121 gtcgcggagg ctggcgagcg atgcaagcgt cttggtgacg tcgtccgcgt tcggcaccgg
      181 ggccgccggc ggggcgggca ccaccggagc tggcgccacc gggccggggg ccggtgccgg
      241 cgcagccgcg gcccgcggcg tgttgagggc gatcatccgc tccgtcttcg actccatcat
      301 ggccttcttg aacccgatcg gatcccgcag cgcgcgcagg ttgttgatcc cggcctcgga
      361 cgcggtcatg acgacgagat cgccgaaccc caggatccgt cccagccacg ggacgcggat
      421 ctgggcgtcg gtgatgttct cgagcgagct gtcaccggac tgcttgttga tcacgccgtg
      481 gacgtggagg acgcgcacgg tcgtgaggat gtactcctgg ctctgccact ggatcacgga
      541 gtagatgaag ccgatcaccg cgaacgccag ggcgatcacc gtgacgatcg tgaccacgtt
      601 gttgatgaag gcgatcgtgc cgccgatgat cccggtcccg tcagatttga acacccactg
      661 cgagaggatg aacccgacga ccgtcacgag gaccgcgacg gcgacccact tgcccgcgat
      721 atagaacgga aagatccagt gctggtgctc ccgccggacg accgtctcgt cggtcgccag
      781 gagcccgtcg gcgtatgaca tggtcgatct ccctcgcgcc cgcggccgtc acccagtcgc
      841 gtccggcggt gacacgatgc ttgcacgcgg gtccggccgg cgcaaggcga tcccgcacgc
      901 tcgggctccg tggcgtgtgg cggtcagccg gcctcgcgtg cggtcgaggt cgccggcacg
      961 gcggccggcg ccaggtgcgt cggctgcatg gcgtcccaca gccgccggcg cccgatggcc
     1021 aggccgagcg cgagcgaggc gagcgcgacg atgccggtcg cgtagaacgg gtaggtgagg
     1081 ttgatggcgg ccagcgcgcc ggcggtgagc gaggccacga tcgtgcccac cgtcccggcc
     1141 gcaccgaaga tgccctgcgc ggtggagctc ctgccggccg gcgaggcccg cgccacgagc
     1201 aggaacaggg ccggcgacgc cagcgcgaac gccgtgccct cgacgatgcc gagggcggcc
     1261 atccaccaga tctcgggcac gagcgggtac agcaggccgc acagcccgac gccggccatg
     1321 ccgatcacga gcgcgaagaa gccaccctca cggtcgatga accggccgaa ccagggcgac
     1381 agcacgagga tcggcagggc gaacgtgaag aacgtcagcc cgatcagccc gaggctcgcg
     1441 ccgaggtggg tgaggtagag gctccagatg acctcgtagg cgccgctcgc gacgtagctg
     1501 ccgatgttga acgcgacggc ggcgatcagg agccggttca cgatgctgcc cggctgtcgc
     1561 ggggcttccg cggcctcggc ggcgggtgcc atgtggcccg cggtcgcggg cgtccggtgg
     1621 gcgagctccg ggacccgggc ggcgacgacg atcgccgaca cgatggtcat cagcccggcc
     1681 acccagaaca cgacggtcgg ctgaccggtg aggccggcgg cgatcccgcc gatcgccggt
     1741 ccgatcatga gcccgcccat ctgggcggcc ccgtagaggc cgaacatctc cccctggcgc
     1801 tcgggcgggt tggcgtcgac gaggtagccg cgggcggcgg ggtcgtagat ggacgcggac
     1861 agcccggaca gcgctcgaag cacgatgaac gctgccggtc cgacgatgaa cagcggcgcg
     1921 acggcgaaca cggcggccag cagcaggccg acgatcatca tcgccttgcg cgggccgcgg
     1981 tcggcgaccc agccgaacag cggctcgccg atgagtcgcg ccgccggcca cgcggcgacg
     2041 acgatcccga gtgtcggcag gtcgaccccg tgctcggtga agtagatcgg gaggatcggg
     2101 aggagcgcgc cgaatccgac ccagatgctc gcttccgcga ggagcatcgg gagcatcggg
     2161 ccccaggcat cgagccagag gcgggcgcgt cgggtcacgg gtcggcctgc ggtagcggag
     2221 ggtcatccta gcggaggacc cgcccttccc ccgtccgtgg accgtgcgcc agaaccgggg
     2281 catatgatcg gtcgatggcg cccctgctgc tcctgtcgat cctcgtcgcc ggtctggtgg
     2341 ccctgctgcc ggtgtggcgg ctgcacgtcg cgggctggcc ggtccgctgg ctgttcaccg
     2401 cctggatcct gtacgcgatc ggcatcctcg cggtgctccg gctgccgggg cttcgcttcg
     2461 tgatcccgat cgtggtcctg gcgtacatcg ccccgttcgt cgccggaccc gagcgggtgt
     2521 cgcgggtgct gagcggtcgc aggacgacgc cagccgtcgt gatcgacgtg accccgcggg
     2581 cggcacccg
//
LOCUS       DBNHXO010000122         8595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3203412, whole genome shotgun sequence.
ACCESSION   DBNHXO010000122 DBNHXO010000000
VERSION     DBNHXO010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 8595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8595
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3203412"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="AC162A_02535"
     CDS             <1..370
                     /locus_tag="AC162A_02535"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR5009742.1"
                     /translation="GGFAVVLELVPGQLAAAITERLTIPTIGIGAGAGCSGQIQVITD
                     TLGWGDWTPKHARRYADLRGTILGAVEQDRADVEAGSFPAEAETVLMDPGVLDEVLGR
                     SVDDQPSTMGIAGIPLDRDL"
     gene            390..1223
                     /gene="panC"
                     /locus_tag="AC162A_02540"
     CDS             390..1223
                     /gene="panC"
                     /locus_tag="AC162A_02540"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR5009743.1"
                     /translation="MEVLRTRAELRAALATASRPVGLVPTMGWLHAGHAALIERARVD
                     NATVVVSIFVNPRQFGETSDFTQYPRNEARDLSICEAAGVDLVFAPTVEEVYPPGFDT
                     TVTMGAIARPLEGAARPGHFDGVATVVAILFALVGAERAYFGLKDYQQVRVIRRMALD
                     LALPTEVVALDTIREPDGLALSSRNARLSPAGRAAAPVIRRALLAGAACVRDGERDAD
                     VVRAEVSRVLATERLAEVDYVSVADPDTLKELATIGGTGALLSMAVRIEGVRLIDNER
                     V"
     gene            complement(1390..2382)
                     /locus_tag="AC162A_02545"
     CDS             complement(1390..2382)
                     /locus_tag="AC162A_02545"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013986.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HIR5009744.1"
                     /translation="MTGVILAVDGGNSKTDVVLCSAEGAVLAAARGPTTSHQAVGLEA
                     GLDRLAALVAAAAGPPADLAVCCLAGLDLAADEARLQPAYAARAVARETLLFNDTIAA
                     LRAGTPDGWGVAVICGSGVNAIGRAPDGRIARFAGLGEIAGDRGGGSGLGMWGLGAAV
                     RAVDGRGPATSLSTLVPAFFGLDEPAAVTEAMYVGRIDQDRVGELAPVVAQAARDGDA
                     VAAELVDDVADELASFATAAIRRLGMEGEAVPVTLAGGLARGAADLLVPRVATLVREI
                     APAADVTVLHAPPVLGAPLLGLDRLAPGDHAAAERLRAAVIAWDAIPGARPAGS"
     gene            complement(2379..2999)
                     /locus_tag="AC162A_02550"
     CDS             complement(2379..2999)
                     /locus_tag="AC162A_02550"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR5009745.1"
                     /translation="MISASPFRVALTFDAEHPDRPHQPGITVGLLDVLAERAVPSTWF
                     LQGRWVEAEPTLARRVAEDGHLVGNHSFYHARLPLLTDDGLVTDIRDAEAVIRDVTGA
                     DPQPWFRCPFSAGDDDPRVLATLAALGYRDVGADVVLEDWEPSRTGPALAADALACTP
                     AVGDGAVVLVHAWPPGTLDALPAIVDGLRAAGATFVRIDALERFTA"
     gene            complement(2996..4111)
                     /locus_tag="AC162A_02555"
     CDS             complement(2996..4111)
                     /locus_tag="AC162A_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5009746.1"
                     /translation="MAAEAGQLDPAASGGVLARLSRSVRPRRPHRPRDAVKSFALTFA
                     LVAVLAAFLAPMLRATLVSLKTPDQLSETNAPFLPSLPQTFSFEGKDRDVYTVPLPDG
                     TTRQLAMVKPGRQQSDFVDPANPAAGTITWQGSWRTLDRAWLLAPAFQNYVDVWNLID
                     FPRLLFNTVALAVIGMIGTVISCTLVAYGFARFRFPGRSLLFTILIATIFLPSAVTVV
                     PTYTMFVQLGWVGTWLPLLVPAFFANAFDVFLIRQYFLTIPREMDEAASIDGAGPFRT
                     LISVILPQAWPAIVAVAIFHLVYAWNDFFGPLIYLSTKPELQPLAVGLQRFNGIHYRN
                     PAYVQAGTLMTMLIPVILFLIFQRIFVRGVVVTGVDK"
     gene            complement(4112..5074)
                     /locus_tag="AC162A_02560"
     CDS             complement(4112..5074)
                     /locus_tag="AC162A_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5009747.1"
                     /translation="MTAVGRTTRPRLRGLPRLRSLSPLTRREALWGYLFISPWIIGFL
                     WFVLGPMIASLAFTFTNINLAQEEPLQFVGLRNWQTMLGDAQVWAALGVTIRFALIQL
                     PVVLILPFLLAIGLNARYVKGASAFRVLFFLPYVVPFVSGVLIWGGMLNSETGWVNEV
                     LRFLGVENPPNWLNDTGWVYPALVMIGIWGIGAGIIVNLAGLRGIPTELYDAARIDGA
                     GFWGALRHITLPMMSPVIFYTLVLAIVEVLQYFLVPLVINNGTGEPGGSTFFFNLYLY
                     KNFFTYQNMAYGATLAWLLFLVTLVVSLIIFRTSRRWVYYAGER"
     gene            complement(5147..6643)
                     /locus_tag="AC162A_02565"
     CDS             complement(5147..6643)
                     /locus_tag="AC162A_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5009748.1"
                     /translation="MRVIRATTAVAVLALVVGACSSGAPTPAPSSAPTVAASDAASQP
                     APPSDGASAPANTPIAIGGSTPAPGQTEIRWYCCLGGGDAPEQVAVEKQVVEAFNASH
                     PNIHVTFEAVPYAGANDALATEIASGNGPDIVGPVGIGGANAFHGQWLDLAPLITKSN
                     YDLSGFPPDAVNIYKLDEGQVGIPFAIYPSVLFYAKSLFDEAGLAYPPHKYGDKYTMP
                     DGSVVDWNYDTITQVAKILTVDSTGKDATQDGFDPTKIVQWGFEPQRDDLRGMGAYFG
                     AGSLAGADGKTAQIPDPWKAAWKHWYDNIWTDHVSMTGPIFQSPDINPEDYPFFTGKV
                     AMSQNFLWSTYGLEGLEGDWDMAALPAYNGKTTSAFNADTFRILKDTKHPDEAFEVLT
                     YLLGEAAPDLLSTYGGMPARTDEQAAFFESQDETFTQKPDWQVAIDSIPFADNPNFEA
                     FMPKYNETLGLVGSGGKYTTRWQTEKGLNMDQEFEALRSEIQAIWDKP"
     gene            complement(6694..7515)
                     /locus_tag="AC162A_02570"
     CDS             complement(6694..7515)
                     /locus_tag="AC162A_02570"
                     /inference="COORDINATES: protein motif:HMM:NF019322.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR5009749.1"
                     /translation="MARFRLLAGPVPLHHQVYLDLRAALEAGEWRPGDRLPPERELAE
                     RYGCSLITVRRALSDLARERRLERTRGRGTFVTRPPIDLDLDGSMSFTEEVQQLGHDP
                     ATRVVTARTVAADAAVAEALDIPLGASVVHLERLRMADGEPLLLEQVFLPEARFPGLL
                     ASDLERGSLYELLGERYDTRVVRARESLEPIALHQREAHLLGVEPHRPALRIEGLAYD
                     RSGRPVEFARSYVRGDRTRYYVERVVVRSNPRSDDIDAGAAGALGVASTSVRRAT"
     gene            7622..>8595
                     /locus_tag="AC162A_02575"
     CDS             7622..>8595
                     /locus_tag="AC162A_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014179641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 4 glycosyl hydrolase"
                     /protein_id="HIR5009750.1"
                     /translation="MGLKVAVIGAGSTYTPELVEGFARREAVLPIDEIALMDPDTDRL
                     EIVGGLAGRILKRAGWPGQLTLTGDRSAALDGAAYTLIQLRVGGQAARLVDETLPLRF
                     GLIGQETTGPGGFAKALRTVPLVLEIAEESARRGASDNWILDFTNPVGVVTQALLDAG
                     HRAIGLCNVAIGFQRSLAKHFDVTPDRVSLDHVGLNHLSWIRAVRVDGVDRLPEMLEA
                     GGDAVTLGGDFPLEVVRALGAIPSYYLHYYYATDDVLRAQQDGASRATDVIHIERELL
                     EMYRDPTLDHKPELLERRGGAYYSEAAAALIESLHTGDGAVHVVDVRNA"
BASE COUNT         1294 a         2956 c         3157 g         1188 t
ORIGIN      
        1 gggggggttc gccgtggtgc tcgagctcgt gccgggccag ctagccgcgg cgatcaccga
       61 gcggctgacg atcccgacga tcggcatcgg cgcgggcgcc ggctgctccg gacagatcca
      121 ggtcatcacc gacaccctgg gctggggcga ctggacgccg aagcacgccc gccgctacgc
      181 ggacctgcgg gggacgatcc tgggcgccgt cgagcaggac cgggccgatg tcgaagcggg
      241 ctctttcccg gccgaggccg agaccgtgct gatggatccc ggggtcctcg acgaggtgct
      301 cggtcgatcg gtggacgacc agccctcgac gatggggatc gccggcatcc cgctcgaccg
      361 cgacctctag accttcggcc gaggggcgcg tggaggtcct tcgcacccgc gccgaactgc
      421 gcgccgcgct ggcaacggcg tctcgtcccg tcgggctggt gccgacgatg ggttggctcc
      481 acgccggcca cgcagcgctg atcgagcggg cacgggtcga caacgcgacg gtcgtcgtct
      541 cgatcttcgt caacccccgc cagttcggcg agacgtcgga tttcacccag tacccgcgca
      601 acgaggcccg cgacctctcg atatgcgagg cggcgggcgt ggacctcgtg ttcgcgccga
      661 ccgtggagga ggtctatccg cccggcttcg acacgacggt gacgatgggc gcgatcgccc
      721 ggccgctcga gggcgctgcg cggcccgggc acttcgacgg cgtggcgacg gtcgtcgcga
      781 tcctgttcgc gctcgtcggc gccgagcggg cgtacttcgg gctcaaggac taccagcagg
      841 tccgggtgat ccgccgcatg gcgctggacc tcgcgctgcc gaccgaggtc gtcgcgctcg
      901 acacgatccg tgagccagac gggctcgccc tctcgtcgcg gaacgcccgg ctgtcgcccg
      961 cgggtcgggc ggccgcgccg gtgatccggc gggcgctgct cgcaggcgcg gcttgtgtcc
     1021 gggacggcga gcgcgacgcc gatgtcgtcc gggccgaggt ctcgcgcgtc ctcgcgacgg
     1081 agcggctcgc cgaggtggac tacgtctcgg tcgcggaccc ggacacgctc aaggagctgg
     1141 cgacgatcgg cggaaccggg gcactgctgt cgatggcagt ccggatcgag ggcgtgcgcc
     1201 tgatcgacaa tgagcgggtc tgaccagctc cgccctctga caaatcctcg atctgtgact
     1261 gcacgtcggg gtccgcgcct cgcccgaggc cgagagttga cgtttacggc taactcgagg
     1321 cgtccgaacc cggctttcgc ctgatctggg cgctcatcgc ctggccaata gtcagcggct
     1381 gcgattctgt caggagccgg cggggcgagc tccggggatg gcgtcccagg cgatgaccgc
     1441 ggcccgcagc cgctccgcgg cggcgtgatc acccggcgcg aggcgatcga ggccgagcag
     1501 cggggcgccc aacacgggcg gcgcgtggag gaccgtgacg tcggcggccg gcgcgatctc
     1561 gcggacgagc gtggcgacgc gcggcacgag caggtccgcg gcgccacggg ccaggccgcc
     1621 ggccagcgtg accggcaccg cctcgccttc catgccaagc cgccggatcg cggccgttgc
     1681 gaacgacgcc agctcgtcgg cgacgtcgtc gacaagctcc gccgcaacgg cgtcgccgtc
     1741 acgggccgcc tgcgctacga ccggcgccag ctcccccacc cggtcctggt cgatccgccc
     1801 gacgtacatc gcctcggtca cggcggctgg ctcgtcgagc ccgaagaacg ccggcaccag
     1861 cgtggacagg gacgtcgccg gccctcgtcc gtcgaccgcc cggacggccg ccccgaggcc
     1921 ccacatgccc agcccggacc cgccgccccg gtcgccggcg atctcgccca gcccggcgaa
     1981 ccgcgcgatc cgcccgtccg gtgcccggcc gatcgcgttg acccccgagc cgcagatcac
     2041 ggccacgccc cagccatcgg gcgtcccggc ccgcagcgcc gcgatcgtgt cgttgaacag
     2101 cagcgtctcg cgggcgaccg cgcgcgccgc ataagcgggc tggagccggg cctcgtcggc
     2161 agcaaggtcg agcccggcta gacaacacac ggcgagatcg gccggcgggc ctgccgcggc
     2221 cgcgacgagc gccgcgagcc ggtcgagccc ggcctcgagc ccgacggcct gatgcgacgt
     2281 cgtgggtccg cgcgccgctg ccagcaccgc cccctcggcc gagcacagca cgacgtcggt
     2341 cttggagttg ccgccgtcga cggcgaggat gaccccggtc acgcggtgaa ccgctcgagc
     2401 gcgtcgatcc gaacgaacgt cgccccggcc gcgcggaggc cgtcgacgat cgcgggcagc
     2461 gcgtcgagcg tgcccggcgg ccaggcgtgg accagcacga cggcgccgtc ccccaccgcg
     2521 ggggtgcacg cgagcgcgtc ggcggccaac gccgggcctg tccgggatgg ctcccagtcc
     2581 tccagcacga cgtcggcacc cacgtcgcgg tagccgagcg ccgccagcgt cgccaggacg
     2641 cgggggtcgt cgtcgcccgc cgagaacggg cacctgaacc agggctgcgg gtccgcgccg
     2701 gtgacatcgc ggatcacggc ctcggcatcg cggatgtcgg tcaccagccc gtcgtcggta
     2761 agcagcggca ggcgggcgtg gtagaagctg tggttcccca ccaggtggcc gtcctcggcg
     2821 acgcgccgcg ccagcgtggg ttcggcctcc acccagcggc cctgcaggaa ccaggtcgac
     2881 gggacggcgc gctctgccag cacgtccagc agaccgaccg tgattcccgg ctggtgcggc
     2941 cggtcggggt gctcggcgtc gaacgtgagc gcgacccgga acggggacgc cgagatcact
     3001 tgtccacccc ggtgaccacg acgccgcgca cgaagatccg ctggaagatc aggaacagga
     3061 tcacagggat gagcatggtc atcagcgtgc ccgcctgcac gtaggccggg ttccggtagt
     3121 ggatcccgtt aaagcgctgc aggcccacgg ccagcggctg gagctcgggc ttcgtcgaga
     3181 gatagatgag cgggccgaag aaatcgttcc aggcgtagac gaggtggaag atcgccaccg
     3241 cgacgatggc cggccaggcc tggggcagga tcaccgagat cagcgtccgg aacggacccg
     3301 cgccgtcgat cgacgccgcc tcgtccatct cccgcgggat cgtgaggaag tactggcgga
     3361 tcaggaacac gtcgaacgcg ttcgcgaaga aggccggcac gagcagcggc agccacgtcc
     3421 ccacccagcc cagctgcacg aacatcgtgt aggtcgggac cacggtgacc gcggacggca
     3481 ggaagatcgt cgcgatcagg atcgtgaaca gcagtgaccg gccggggaac cggaaccggg
     3541 cgaagccgta ggcgaccagc gtgcacgaga tgacggtccc gatcatcccg atgaccgcga
     3601 gcgcgaccgt attgaacagg agccggggga agtcgatcag gttccacacg tcgacgtagt
     3661 tctggaacgc cggcgccagg agccaggcgc ggtccagcgt ccgccacgag ccctgccagg
     3721 tgatcgtgcc ggctgccggg ttggcggggt cgacgaagtc gctctgttgg cggccgggct
     3781 tgaccatcgc cagctggcgg gtggtgccgt cggggagcgg aacggtgtag acgtcgcgat
     3841 ccttgccctc gaagctgaac gtctgcggca aggagggcag gaacggcgcg ttggtctcgg
     3901 acagctggtc cggcgtcttg agcgagacca gcgtcgcgcg cagcatcggc gccaggaacg
     3961 cggcgaggac ggcaaccagc gcgaacgtca gcgcgaacga cttgacggcg tcacgcggcc
     4021 gatggggccg gcgtggccgt accgaccgcg acagccgggc gagcacgcct ccggaggcag
     4081 ccgggtcgag ttggccggcc tcggcggcca cctagcgctc ccccgcgtag tacacccagc
     4141 ggcgcgacgt gcggaagatg atcagcgaca ccaccagggt caccaggaac agcagccacg
     4201 cgagcgtcgc cccgtaggcc atgttctggt aggtgaagaa gttcttgtag aggtagaggt
     4261 tgaagaagaa cgtcgagccg ccgggctccc ccgtgccgtt gttgatgacc agcgggacga
     4321 ggaagtactg gagcacctcg acgatcgcga gcaccagcgt gtagaagatg accggcgaca
     4381 tcatcggcag cgtgatgtga cgcagcgcgc cccagaagcc cgcaccgtcg atgcgcgcgg
     4441 cgtcgtacag ctcggtcggg atcccgcgca ggccggcgag gttgacgatg atcccggcgc
     4501 cgatgcccca gatgccgatc atgaccaggg ccgggtagac ccagccggtg tcgttgagcc
     4561 agttgggcgg gttctcgacg cccaggaacc ggagcacctc gttcacccag cccgtctcgg
     4621 agttgagcat cccgccccag atgagcacgc ccgacacgaa cgggacgacg tagggcagga
     4681 agaacagcac ccggaacgcg ctggcgccct tgacgtagcg ggcgttgagg ccgatggcca
     4741 gcaggaacgg caggatgagg acgacgggca gctggatgag ggcgaaccgg atcgtgaccc
     4801 cgagggccgc ccagacctgc gcgtcgccca gcatcgtctg ccagttgcgc aggccgacga
     4861 actggagcgg ctcctcctgc gcgaggttga tgttcgtgaa cgtgaacgcc agcgacgcga
     4921 tcatcggccc cagcacgaac cacaggaagc cgatgatcca gggcgagatg aacaggtagc
     4981 cccacagcgc ctcgcgacgc gtgagcggtg agaggctccg gagcctcggc agcccgcgaa
     5041 gccggggccg ggtggtgcgg ccgacggccg tcacggggcg cgcaggggca cgatcatctg
     5101 gacctcactg gcggcaccgg cggggccggt gccgccgaat cgcaggttac ggcttgtccc
     5161 agatcgcctg gatctcggag cgcagcgcct cgaactcctg gtccatgttc aggccctttt
     5221 cggtctgcca cctggtcgtg tacttgccgc ccgagcccac gaggccgagg gtctcgttgt
     5281 acttgggcat gaacgcctcg aagttggggt tgtccgcgaa cgggatgctg tcgatcgcga
     5341 cctgccagtc cggcttctgg gtgaaggtct cgtcctggct ctcgaagaac gcggcctgct
     5401 cgtcggtccg ggcgggcatg ccgccgtagg tcgacaggag gtcgggggcc gcctcgccga
     5461 ggaggtaggt caggacctcg aacgcctcgt cgggatgctt ggtgtccttg aggatgcgga
     5521 aggtgtcggc gttgaacgcg gaggtcgtct tgccgttgta cgcgggcagc gcggccatgt
     5581 cccagtcgcc ctcgagccct tcgaggccgt atgtcgacca caggaagttc tggctcatcg
     5641 cgaccttgcc ggtgaagaac ggatagtcct cggggttgat gtcggggctc tggaagatcg
     5701 gcccggtcat gctcacgtgg tcggtccaga tgttgtcgta ccagtgcttc caggcggcct
     5761 tccagggatc cgggatctgg gcggtcttgc catccgcccc ggccagcgag ccggcgccga
     5821 agtaggcgcc catgccccgc aggtcgtcgc gctggggctc gaagccccac tggacgatct
     5881 tggtcgggtc gaagccgtcc tgagtcgcgt ccttgccggt cgaatcgacg gtcaggatct
     5941 tggcgacctg cgtgatggtg tcgtagttcc agtcgacgac cgagccgtcg ggcatcgtgt
     6001 acttgtcgcc gtacttgtgg ggcgggtacg cgagtccggc ctcgtcgaac aggctcttgg
     6061 catagaacag gacggacggg tagatcgcga acgggatgcc gacctggccc tcgtcgagct
     6121 tgtagatgtt gaccgcatcc ggcgggaagc ccgacaggtc gtagttgctc ttcgtgatga
     6181 gcggcgcgag gtccagccac tggccgtgga aggcgttggc gccgccgatg ccgacggggc
     6241 cgacgatgtc cggaccgttg cccgaggcga tctcggtcgc gagcgcgtcg ttggcgccgg
     6301 cgtacgggac ggcctcgaac gtcacgtgga tgttggggtg gctggcgttg aacgcctcga
     6361 ccacctgctt ctcgaccgcg acctgctccg gggcgtcgcc accgccgagg cagcagtacc
     6421 agcggatctc ggtctggccg ggtgccggcg tcgaaccgcc gatcgcgatc ggggtgttcg
     6481 ccggggccga tgcgccgtcg gacggcgggg ccggctggga cgcggcatcg gatgccgcta
     6541 cggtcggcgc ggacgatggc gcgggcgtcg gggccccgct gctgcaggcg ccgacgacca
     6601 gcgccagcac ggcgacggcg gtcgtggctc ggatcactcg catctgctcc tcctccggaa
     6661 cacgggaccg ccgccggtcg cggcagctgc tccctacgtg gctcggcgaa ccgaggtgct
     6721 ggcgacgccc agggcgccgg cggcacccgc gtcgatgtcg tcggatctgg ggttggagcg
     6781 gacgacgacc cgctccacgt agtagcgcgt ccggtcgccc cggacgtacg aacgcgcgaa
     6841 ctcgaccggc ctgcccgacc ggtcgtacgc gagcccctcg atgcgcagcg ccggccggtg
     6901 cggctcgacg cccaggaggt gggcctcccg ctggtgcagg gcgatcggct cgagcgactc
     6961 ccgcgcgcgg accacccgcg tgtcgtagcg ctcgcccagg agctcgtaca gggagccgcg
     7021 ctcgaggtcg ctggccagca ggccagggaa cctcgcctcg ggcaggaaga cctgctcgag
     7081 gagcagcggc tcgccgtcgg ccatgcgcag ccgctcgagg tggacgacgg acgcgccgag
     7141 cgggatgtcg agcgcctcgg cgacggcggc gtccgcggcg acggtgcgcg cggtgacgac
     7201 gcgggtcgcc gggtcgtgac ccagctgctg gacctcctcg gtgaacgaca tcgagccgtc
     7261 gaggtcgaga tcgatcggcg ggcgggtgac gaacgtgccc cgcccgcgcg tccgctcgag
     7321 ccggcgttca cgcgccaggt cgctgagcgc ccggcggacg gtgatcaggc tgcagccgta
     7381 gcgctcggcg agctcgcgct cgggaggcag ccggtcgccc gggcgccact cgcccgcctc
     7441 gagcgccgcg cgcaggtcaa gatagacctg atggtggagc gggacgggtc ccgccaggag
     7501 ccggaagcgt gccatgcccg tcgcgagtcc tcgtgcggtg ggacgtggtg ggggcttgcg
     7561 gtcggcgaag ttgctgctaa cgttataagc aatctgtcaa gcccgacgca aggggtggtc
     7621 gatgggcctc aaggtcgcgg tgatcggtgc gggcagcacc tacacgcccg agctcgtcga
     7681 gggatttgcg cggcgcgagg ccgtgctgcc gatcgacgag atcgcgctga tggacccgga
     7741 caccgaccgg ctcgagatcg tcggcgggct ggcgggccgg atcctcaagc gagccgggtg
     7801 gcccgggcaa ttgacgctca cgggtgaccg gtcggccgcc ctcgacggcg ccgcgtacac
     7861 gctgatccag ctccgcgtcg gcggccaggc cgcgcgcctc gtcgacgaga ccctgccgct
     7921 gcggttcggc ctcatcggcc aggagaccac cgggcccggc gggttcgcga aggccctgcg
     7981 cacggtcccg ctggtcctcg agatcgccga ggagtcggcg cggcgcggcg catcggacaa
     8041 ctggattctc gacttcacca atccggtcgg cgtcgtcacc caggccctgc tcgacgccgg
     8101 ccatcgcgcc atcggcctgt gcaacgtggc gatcgggttc cagcgcagcc tggcgaagca
     8161 cttcgacgtc acgcctgacc gggtctcgct cgaccacgtg ggcctgaacc acctgtcctg
     8221 gatccgggcg gtgcgggtgg acggcgtgga ccggctgccc gagatgctcg aggccggcgg
     8281 cgacgccgtg acgctcggcg gggacttccc gctcgaggtc gtccgggcac tgggcgcgat
     8341 cccctcctac tacctgcact actactacgc gacggacgac gtcctccggg cgcagcagga
     8401 tggcgcgagc cgcgccaccg acgtgatcca catcgagcgc gagctgctcg agatgtaccg
     8461 cgacccgacg ctcgaccaca agcccgagct gctcgaacgc cgcggcggcg cgtactacag
     8521 cgaggcggcg gcggcgctca tcgagtcgct ccacaccggc gacggggccg tccacgtggt
     8581 cgacgtccgc aatgc
//
LOCUS       DBNHXO010000123         3138 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3203691, whole genome shotgun sequence.
ACCESSION   DBNHXO010000123 DBNHXO010000000
VERSION     DBNHXO010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3203691"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="AC162A_02580"
     CDS             <1..446
                     /locus_tag="AC162A_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5009751.1"
                     /translation="LLWPTTFLSIHPDYLLVHRLVPVSPGHTTVICDWLFEGETIDAE
                     GFDPSDAVAFWDLTNQQDWHVCEMQQRGTSSRSWIAGRYSNEEASVQAFDLMVVDRYA
                     EDGVASNRTVRERYDLPPPKPDGNGNGRRVTVAELAREGAAASRG"
     gene            complement(453..1259)
                     /locus_tag="AC162A_02585"
     CDS             complement(453..1259)
                     /locus_tag="AC162A_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009752.1"
                     /translation="MRRDRNRWLTAYSIAVYVFLFLPIAILILFSFNASRHNFAWVGP
                     TLRWYPRLFRNEELIDALIVTLEVAVVAVIVATVLGTLLGLGLTRLNQRRRASAEALL
                     LLPMVTPEIVMGISMLLFFWQAFGGQGSIQQISIAHITFCISYVAVVVRARAANLDPQ
                     LEEAARDLGSSAWGAFRHVTFPLLLPAVAAGAMLTFALSFDDLIVTTFNAGVGSSTLP
                     LFIYSKIRFGVTPEINAISTIIVSITAILIFVAWRMGGLRGETKAGVAGV"
     gene            complement(1259..2107)
                     /locus_tag="AC162A_02590"
     CDS             complement(1259..2107)
                     /locus_tag="AC162A_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009753.1"
                     /translation="MRSRFGAGPLVLPGILWLGLFFLLPLAIIFVVSLGTKDRFGVVH
                     FDTLTLEHYATALSPTFLPTVWNSFRYAALTTIFSIAIGYPIAYWISRYGGRNKVLLL
                     ILVMLPFWTSYLIRTYAWMIILRDNGVVNSVLQAIGLVSPNDPIILLNTDLSVILGMT
                     YGFLPFAILPLYVSIDRLDDSLVSAARDLYASGRQAFRYVTLPLTMPGIIAAAILTFI
                     PAIGDYVTPELLGGAETTTIAKVVQSQFLSARDWPSGAALGFLLMVVTIIGTLVSLRV
                     LRREVL"
     gene            complement(2100..>3138)
                     /locus_tag="AC162A_02595"
     CDS             complement(2100..>3138)
                     /locus_tag="AC162A_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5009754.1"
                     /translation="TTPGSSAAASGEPTATPAPTPAPTPEGELFIYNWALYMGKSVIP
                     SFEKKTGIKVREDFFDNYDTALAKVGQAGGGYDIFFPASTEIPSLIERGLMQPLDHSL
                     LPNITNLGAEWADPGYDPGNAHSIPYMWWTTGVAYDTAKVPETLTSWNALWDEKYKKH
                     IAVLDDQREAFAAALFLLGKDPNTTNDADLDAALALLQEQKPLVRVYTTDDIGVLSTG
                     DAWVAHAWGSDVYQIVTERPTVKFYIPEEGGVRGSDATVLLANAKHPIAAQMFMNHLL
                     DAKVSARNTNYIGYMGPNAAAKEFIKPYILNDPAVNPDQAIVDKLFELLDLGADLDKY
                     ATRWTTLRSGA"
BASE COUNT          549 a          993 c         1119 g          477 t
ORIGIN      
        1 acctcctgtg gccgacgacg ttcctctcga tccacccgga ctacctgctg gtccatcggc
       61 tggtcccggt cagcccgggt cacacgacgg tgatctgcga ctggctgttc gagggcgaga
      121 ccatcgatgc cgagggcttc gacccgtccg acgccgtggc gttctgggac ctgaccaacc
      181 agcaggactg gcacgtgtgc gagatgcagc agcgcggcac gtcgtcgcgc agctggatcg
      241 ccggccgcta ctccaacgag gaggcgtcgg tccaggcgtt cgacctgatg gtggtcgacc
      301 gctacgccga ggacggcgtg gccagcaacc ggaccgtccg ggagcgctac gacctcccgc
      361 cgccgaagcc ggacgggaac ggcaacggac gccgggtgac cgtggccgag ctggcccgtg
      421 aaggggccgc cgccagccgg ggctgaccgg agtcagacgc ccgcgacgcc ggccttcgtc
      481 tcgccgcgga ggccgcccat gcgccaggcg acgaagatca ggatcgccgt gatcgaaacg
      541 atgatcgtcg agatcgcgtt gatctccggc gtcaccccga accggatctt ggagtagatg
      601 aacagcggca gcgtcgagga cccgacgccc gcgttgaacg tcgtcacgat caggtcgtcg
      661 aacgagagcg cgaacgtcag catcgcgccg gccgcgaccg cgggcagcag gagcgggaac
      721 gtcacgtggc ggaaggcgcc ccacgccgac gacccgaggt cgcgcgcggc ctcctccagc
      781 tgcgggtcga ggttcgcggc gcgcgcgcgg accacgaccg ccacgtacga gatgcagaac
      841 gtgatgtggg cgatcgagat ctgctggatc gacccctggc caccgaacgc ctgccagaag
      901 aacagcagca tgctgatgcc catgacgatc tccggggtca ccatcggcag cagcaggagc
      961 gcctcggcgg acgcgcgcct ccgctggttc aggcgggtca gcccgaggcc gagcagcgtg
     1021 cccaggacgg tggccacgat gaccgcgacc acggccacct cgagcgtcac gatcagcgcg
     1081 tcgatcagct cctcgttgcg gaacagccgc ggataccagc gcagcgttgg ccccacccac
     1141 gcgaagttgt ggcgcgaggc gttgaacgag aacaggatga ggatcgcgat cggcaggaac
     1201 aggaacacgt agaccgcgat cgagtaggcg gtcagccagc ggttccggtc ccggcgcact
     1261 acaggacctc gcggcgcagc acgcgcaggc tcacgagggt cccgatgatc gtgaccacca
     1321 tcagcaggaa tccgagcgcg gcgcccgatg gccagtcacg cgccgaaagg aactgcgact
     1381 ggacgacctt cgcgatcgtc gtggtctcgg cgccacccag gagctcgggg gtgacgtagt
     1441 cgccgatcgc cgggatgaat gtaaggatcg ccgccgcgat gatccccggc atcgtgagcg
     1501 gcagggtcac gtaccggaac gcctggcggc cgctggcgta caggtcgcgg gcggcggaca
     1561 cgagcgaatc gtcgaggcgg tcgatcgaga cgtacagggg caggatcgcg aacggcagga
     1621 acccgtaggt catgcccagg atcacggaca ggtcggtgtt gagcaggatg atcgggtcgt
     1681 tgggactgac gagcccgatc gcctggagca cggagttcac gaccccgttg tcgcgcagga
     1741 tgatcatcca ggcgtaggtc cggatcaggt agctcgtcca gaacgggagc atgaccagga
     1801 tcaggagcag caccttgttg cggccgccgt agcggctgat ccagtaggcg atggggtagc
     1861 cgatcgcgat cgagaagatc gtggtcagcg ccgcgtagcg gaacgagttc cagaccgtgg
     1921 gcaggaacgt cggggacagc gcggtcgcat agtgctcgag ggtgagggtg tcgaagtgga
     1981 cgaccccgaa gcggtccttg gtcccaaggc tgacgacgaa gatgatcgcc agcggcagca
     2041 ggaagaacag gcccagccag aggatccccg gcaggacgag cggaccggcg ccgaagcggc
     2101 tacgcaccgg agcggagggt cgtccagcgt gtcgcgtact tgtcgaggtc cgcacccagg
     2161 tcgagcagct cgaacagctt gtcgacgatg gcctggtcgg gattcaccgc cgggtcgttg
     2221 aggatgtacg gcttgatgaa ttccttggcc gcggcgttgg ggcccatgta gccgatgtag
     2281 ttcgtgttcc tcgcgctgac cttggcgtcg aggaggtggt tcatgaacat ctgggccgcg
     2341 atcggatgct tcgcgttcgc gagcagcacc gtcgcgtccg agccgcggac gccgccctcc
     2401 tcggggatgt agaacttgac ggtcgggcgc tcggtcacga tctggtagac gtcagagccc
     2461 caggcgtgcg cgacccacgc atcaccggtc gagagcacgc cgatgtcgtc ggtcgtgtag
     2521 acccggacga gcggcttctg ctcctgcagc agggcgagcg ccgcatcgag atccgcgtcg
     2581 ttggtcgtgt tcgggtcctt gcccagcagg aacagggccg ctgcgaacgc ctcgcgctgg
     2641 tcgtcgagca cggcgatgtg cttcttgtac ttctcgtccc acagcgcgtt ccagctggtc
     2701 agcgtctcgg gcaccttggc cgtgtcgtag gcgacgccgg tcgtccacca catgtacggg
     2761 atcgagtgcg cgttgccggg gtcgtaaccc gggtccgccc attcggctcc gaggttggtg
     2821 atgttcggca acagcgagtg gtcgaggggc tgcatgagcc cacgctcgat caggctcggg
     2881 atctcggtcg aggccgggaa aaagatgtcg tagccgccgc cggcctgacc gaccttggcg
     2941 agcgcggtgt cgtagttgtc gaagaagtcc tcgcggacct tgatgccggt cttcttctcg
     3001 aacgagggaa tgacgctctt gcccatgtac agggcccagt tgtagatgaa cagctcaccc
     3061 tcgggcgtgg gcgccggcgt cggcgcgggc gtggccgttg gctcgcccga tgccgccgcc
     3121 gacgatcccg gcgtggtc
//
LOCUS       DBNHXO010000124         5558 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1815258, whole genome shotgun sequence.
ACCESSION   DBNHXO010000124 DBNHXO010000000
VERSION     DBNHXO010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1815258"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="AC162A_02600"
     CDS             complement(<1..1488)
                     /locus_tag="AC162A_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009755.1"
                     /translation="MAVRLQLKLGAVTEQDQLADSPDAIVVVEPSVGSVARTKGNLYL
                     LVTSRTTGNRAREATRMIADTIRNEYYYDESAGIRQCLTKVMGVANKRLAHQRDRYGM
                     GHTGDDGGPIGVAVAVVRGRELYVATVGPAEAYLIRQARLSTLPDPNRDRGLPVEELV
                     PEVWRGELSVGDSLCLVSANIVAKIGTDALKDALVTLHPQSAIEHLHARFVAADGKGS
                     DGALAFEANEVGATHKAKSLVPVRPAEPLAGAPDKSPIPLADTVTASAAAIGAGATRA
                     RSAAGNGFERAIWRLQDLMPRRKSASRKVTPTASRMEMQRRAAVALLAFVAVAGALVI
                     GVYAVGGQRTPTNAIKSLNAAQSAFETAKAALASVSGPGVDLIANDPGRALELLNSAY
                     QQLGEAETAGYPIEQIAPLRATALKGLDRLYGVTRVKSTPMYTFPADKPVELAALVRG
                     DDGAPYVLDTANKTVWRIDLAKKTATAIAKTGQRTSSGRVADPKLL"
     gene            complement(1576..1947)
                     /locus_tag="AC162A_02605"
     CDS             complement(1576..1947)
                     /locus_tag="AC162A_02605"
                     /inference="COORDINATES: protein motif:HMM:NF017103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HIR5009756.1"
                     /translation="MTGPDRESLQRRRRPDRVGDLLPDAARQLGLEAELRFARAIATW
                     DAIVAERVPPAVGACRLERVEPDALVVGVDEPIVAAELRMRALELLAAFAAAPGGSRA
                     RELRLRIGPGGSPGPSGSPRV"
     gene            complement(1957..3117)
                     /gene="recF"
                     /locus_tag="AC162A_02610"
     CDS             complement(1957..3117)
                     /gene="recF"
                     /locus_tag="AC162A_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014516.7"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR5009757.1"
                     /translation="MAPILLGLSVRDLRGYAELDAVFPPGPQLVWGPNAAGKTSLLEA
                     IVLAAWGRSHRTTADGELVRWGQELARVEARVGEPDTDVGARDTVEVALVRTATGGAR
                     KRIRVNGVARRAASLVGVLRTVMFAPEEMLLVAGSPSLRRDALDRLAGQRSPAYLRDL
                     ATYGRALQQRNGLLRLIREEQATRAELRFWDVTLLDAGGAVRAERQRLLDALAEPLAA
                     AHAEIAPDEAAAGRLVVSYATNAPQLPGESARDALARRLAETAEKEVWNGTTLIGPHR
                     DDLVFEMSGRPLASFASRGQQRTAILAFKLAELDLLTALDGRPPLLLLDDVFSELDPE
                     RRAHLVRRIAALPQAFVTTTTLEDLDPALRSVGTAWAVTVAADATSRLEPGR"
     gene            complement(3192..4307)
                     /gene="dnaN"
                     /locus_tag="AC162A_02615"
     CDS             complement(3192..4307)
                     /gene="dnaN"
                     /locus_tag="AC162A_02615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR5009758.1"
                     /translation="MKLSVMQENLARGLSVVSRAVSSRSTLPVLANVLLRTEDAGLKL
                     TATNLEIGITYWVPGKIETDGATTVPARLLTDLVGSLPASEKVDLELQGADTLHLRCG
                     RFATHVKGIDADEFPTIQTAGERPTTRVSQKVLKQALEEVIFAAASDEARPILTGVLA
                     KFEGSKLTLAAADNYRIAVRTIEVLDAVEAASVVIPARALGELARILSDTDDPVELVL
                     AQARNQVLFHLEGIDLVSRLIDGQFPNYQTVLPATHATRAELDREELLRAVRPAALIA
                     SSSANIVKLQVGGDDGGVVVTATADVGDYTGDVEASIEGDATTIAFNARYLNDVLGNV
                     QADRFALELNGPLSPGVFRPVGDDAYVHVVMPVRTTS"
     gene            complement(5058..>5558)
                     /locus_tag="AC162A_02620"
     CDS             complement(5058..>5558)
                     /locus_tag="AC162A_02620"
                     /inference="COORDINATES: protein motif:HMM:NF019903.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5009759.1"
                     /translation="AILRAKAEEQGVPITSDVIEFIARKVVSNIRELEGALNRIVAYA
                     SMGAMPVSIELAQAVLSNVLYNPKTRQVTPERITQAVSDYYGVAMEQLRGQKREKSIV
                     VPRQIAMFLMREETDVSLLRIGAELGGRDHSTVLHACDKITREMQVNDEMRREVAAVR
                     EMIYSE"
BASE COUNT          736 a         1981 c         2025 g          816 t
ORIGIN      
        1 gagcagcttc gggtcggcga cgcgccccga ggacgtccgc tgacccgtct tcgcgatcgc
       61 ggtcgccgtc ttcttggcca ggtcgatacg ccacacggtc ttgttggcgg tgtcgaggac
      121 atacggcgcg ccgtcgtcgc cgcggaccag cgcggccagc tcgaccggct tgtccgccgg
      181 gaacgtgtac atcggcgtcg acttgacccg ggtcacgccg tacagccggt cgaggccctt
      241 gagggcggtc gcccgcagcg gagcgatctg ctcgatcggg tagccggccg tctccgcctc
      301 gccgagctgc tggtaggccg agttgaggag ctcgagggcg cggcccggat cgttggcgat
      361 gagatccacc ccggggccgc tcacgctggc cagggctgcc ttggcggtct caaaggcgct
      421 ctgcgcggcg ttcagcgact tgatcgcgtt ggttggcgtg cgctggcccc cgacggcata
      481 cacgccgatc acgagggcgc cagccaccgc gacgaacgcc agcagcgcca ccgcggcgcg
      541 tcgttgcatc tccatgcgcg acgcggtcgg cgtcaccttg cgcgaagcgg acttgcgcct
      601 gggcatgagg tcctgcagcc gccagatcgc ccgctcaaag ccgttgccgg cggcgctgcg
      661 agcccgcgtt gcgccggccc cgatcgccgc cgcgctcgcg gtgaccgtgt cagcgagcgg
      721 gatgggcgac ttgtcgggcg ctcccgcgag cggctccgcc ggccggaccg ggacgaggga
      781 cttggccttg tgcgtcgcgc cgacctcgtt ggcctcgaag gcgagggcgc cgtcgctgcc
      841 cttgccatcg gccgcgacga accgcgcgtg gaggtgttcg atcgccgact gcgggtgcag
      901 cgtgaccagc gcgtccttga gcgcgtcggt cccgatcttg gccacgatgt tggccgacac
      961 gaggcacagc gagtcgccga ccgagagctc gccccgccag acctcgggca cgagctcctc
     1021 gaccgggagg ccgcggtcgc gattggggtc ggggagtgtc gacaaccgcg cctggcggat
     1081 caggtaggcc tccgccgggc cgaccgtcgc cacgtacagc tcccggccac gaacgaccgc
     1141 cacggccacg ccgatggggc ctccgtcgtc gcccgtgtgg cccatcccgt accggtcgcg
     1201 ctggtgggcc agccgcttgt tggcgacccc catcaccttg gtcaggcact ggcggatgcc
     1261 cgcggactcg tcgtagtagt actcgttgcg gatcgtgtcg gcgatcatcc gcgtcgcctc
     1321 gcgagcgcgg ttgcccgtgg tccgggacgt gaccagcagg tacaggttgc ccttggtacg
     1381 cgccacggag ccgacggacg gctcgacgac cacgatggcg tcgggcgagt cggcgagctg
     1441 gtcctgctcg gtgacggcac cgagcttcag ctggaggcgg acggccaaga aacgagcctc
     1501 atgggtggag cgacgcgcgt cggcatacgg cggtgacggg acggaatccc gtcggcccgt
     1561 cggcgggagt ccggattata cgcgtgggct cccgctcggc ccgggcgagc ctccgggccc
     1621 gatccgcagg cgcagctcgc gtgcccgcga gccccctgga gcggccgcga acgccgccag
     1681 gagctcgagc gcgcgcatcc gcagctctgc cgccacgatc ggctcgtcga cgcccacgac
     1741 cagcgcatcc ggctccacgc gctctagccg gcaggcaccg acggccggcg gcacccgctc
     1801 cgcgacgatc gcgtcccagg tcgcgatggc tcgggcgaac cggagctccg cctcgaggcc
     1861 cagctggcgg gcggcgtcgg gcagcaggtc gccgacgcgg tcggggcgcc ggcgccgctg
     1921 cagggactcg cggtcggggc ctgtcatcgc acggtcctat cgcccgggct cgaggcgcga
     1981 ggtggcgtcg gccgccaccg tgaccgccca cgcggtgccg accgagcgga gcgcagggtc
     2041 caggtcctcg agcgtggtcg tcgtcacgaa cgcctgcggg agcgctgcga tccggcgcac
     2101 gaggtgcgcg cggcgctcgg ggtcgagctc ggagaacacg tcgtcgagca ggagcagcgg
     2161 cgggcggccg tcgagggccg tgagcaggtc cagctctgcg agcttgaacg cgaggatcgc
     2221 ggtccgctgc tgcccgcgcg aggcgaacga cgccagcggg cgtccgctca tctcgaacac
     2281 gaggtcatcc cgatgcgggc cgatgagcgt ggtgccgttc cacacctcct tctcggccgt
     2341 ctcggcgagc cggcgggcga gggcgtcgcg tgccgactca cccggcagct gcggtgcgtt
     2401 ggtcgcgtag ctcaccacga gccgcccggc ggccgcctcg tcgggcgcga tctcggcgtg
     2461 ggcggccgcg aggggctccg cgagcgcatc gagcaggcgc tgccgctcgg cgcggacggc
     2521 gccgccggcg tcgagcaacg tgacgtccca gaaccggagc tccgcccggg tcgcctgctc
     2581 ctcgcggatc agccgcagca gcccgttccg ctgctggagg gctcgcccgt acgtcgcgag
     2641 atctcgcagg taggccggcg accgctgtcc ggccaggcgg tcgagggcgt cgcggcgcaa
     2701 cgacggcgag ccggccacga gcagcatctc ctcgggcgcg aacatcacgg tccgcaggac
     2761 gccgacgagc gatgcggcac ggcgggcgac cccgttgacg cggatccgct tgcgggcgcc
     2821 gccggtggcc gtccgcacga gcgcgacctc gaccgtgtcg cgggcgccga cgtccgtgtc
     2881 cggctcgccg acccgggcct ccacgcgggc cagctcctgg ccccagcgca cgagctcacc
     2941 gtcggcagtc gtgcgatggg agcggcccca ggcggcgagg acgatcgcct cgagcaggct
     3001 cgtcttgccg gcggcgttcg gcccccagac cagctgcggg cctggcggga agacggcgtc
     3061 gagctccgca tagccgcgaa ggtcgcggac cgagagtccg agcaggatcg gcgccatgcg
     3121 cccgctcccc gtggcctcga cgccggaggc gctcgacggt gcgtccgtgg gcgttgcccg
     3181 caggacgtcc gtcaggaggt cgtccggacg ggcatcacga cgtggacgta ggcatcatcg
     3241 cccaccggtc ggaacacgcc cggcgacagc gggccgttga gctcgagcgc gaaccggtcg
     3301 gcctgcacgt tgcccaggac gtcgttgaga tagcgcgcgt tgaaggcgat cgtggtcgcg
     3361 tcgccctcga tcgaggcctc gacgtcgccc gtgtagtcgc ccacgtcggc cgtcgcggtc
     3421 acgacaaccc cgccgtcgtc gccgcccacc tggagcttga cgatgttggc cgacgagctg
     3481 gcgatcagcg ccgccgggcg gacggcccgc aggagctcct cgcggtcgag ctcggcccgc
     3541 gtggcgtgcg tggccggcag gaccgtctgg tagttcggga actggccgtc gatcaggcgc
     3601 gagacgaggt cgatcccctc gaggtggaac aggacctggt tgcgcgcctg ggcgagcacg
     3661 agctccaccg ggtcatcggt gtcggagagg atccgcgcca gctcgccgag cgcccgggcg
     3721 gggatcacga cggacgccgc ctcgaccgcg tccaggacct cgatcgtccg gaccgcgatg
     3781 cggtagttgt cggcggccgc gagggtgagc ttgctgccct cgaacttggc cagcacgccg
     3841 gtcaggatgg gccgcgcctc gtcgctggcg gcggcaaaga tcacctcttc cagcgcctgc
     3901 ttgagcacct tctgcgagac gcgcgtagtc ggacgctcgc ccgccgtctg gatcgtcggg
     3961 aactcgtcgg cgtcgatgcc cttcacgtgc gtcgcgaacc gtccgcagcg caggtggagc
     4021 gtgtcggcgc cctggagctc gaggtccacc ttctcggatg ccggcagcga gccgacgagg
     4081 tcggtcagca gcctggccgg gaccgtggtg gccccgtccg tctcgatctt gcccggcacc
     4141 cagtaggtga tgccgatctc gaggttggtc gccgtgagct tgaggccggc gtcctccgtc
     4201 cgcagcagga cgttggcgag cacgggcagg gtgctccggc tggagaccgc gcgcgacacg
     4261 accgacaggc cgcgggcgag gttctcctgc atcaccgaga gtttcacgta ggttctctcc
     4321 agcatcttct ctgggcgggc gagcctcgcg cggccccgtc gaccggctcc cggtgtgacg
     4381 gcgaagggct tgttccgcga actgccggca ggtggaatga tcagcctatc ttctcatccc
     4441 gtcatcaccg tcatcatgcc gtggattcgg tggatgccaa cgctcaatcg tgcggtcgcg
     4501 gaccgtggat gacccaccgc cgatcgtgga cccggacggg cgtctcgggg atgggaccgg
     4561 ggttcgaggg tggacggtca aagcctggcg cgcggcaatg tccaccgacc cttccgaatc
     4621 gcccgattct tccccgaagc catccccggt tccgggtgtc gccgtccacg ctacgaagcg
     4681 gttgtccgcg cgttgtcccc ggacttgtcc ccaggtcggt gtcggctgcc ggcgcggcca
     4741 ctcccgcggc gcagccgctc cacgactgcc cccagcgcaa cgcgcccgac aggcaccggc
     4801 gcaggggcgc cggcggactc ccagggcccc cgatcggcgc tcatcggagc cctttcatgc
     4861 gttcgatggc atggccggac ccggaagcgt tcggcaggac ggaaggcagg caaccttgcg
     4921 ctcgcagttc cgccgtgtgc atgcatggac cgactgtggc cacagcctga cccggggacg
     4981 gaccgaggaa cggggcgggc gggggacccg gcgctcatca gtgcctggct gccccgttcg
     5041 cggcgaaggc ccgcggccta ctcgctgtag atcatctcgc ggacggccgc gacctcgcgg
     5101 cgcatctcgt cgttgacctg catctcgcgt gtgatcttgt cgcacgcgtg gagcacggtg
     5161 gagtgatcgc ggccgccgag ctcggcgccg atccgcagga gcgagacgtc cgtctcctcg
     5221 cgcatcagga acatcgcgat ctgtcgcggc accacgatgc tcttctcgcg cttctggccc
     5281 cgcagctgct ccatcgcgac gccgtagtag tcggacaccg cctgcgtgat ccgttccggc
     5341 gtgacctggc gcgtcttggg gttgtagagc acgttcgaga ggaccgcctg ggcgagctcg
     5401 atcgagacgg gcatcgcgcc catcgaggcg taggcgacga tccggttcag cgcgccctcg
     5461 agctcccgga tgttggacac gaccttgcgg gcgatgaact cgatcacgtc cgacgtgatc
     5521 ggcacgccct gctcctcggc cttcgcgcgc aggatcgc
//
LOCUS       DBNHXO010000125         1606 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_624464, whole genome shotgun sequence.
ACCESSION   DBNHXO010000125 DBNHXO010000000
VERSION     DBNHXO010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_624464"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="AC162A_02625"
     CDS             <1..999
                     /locus_tag="AC162A_02625"
                     /inference="COORDINATES: protein motif:HMM:NF014090.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR5009760.1"
                     /translation="MRVRVIILVARLFGTRSVSDLVQALEGDEEAIYDTQGDSPDIEA
                     IAADEREHAEIWRRLDGEEGTDGRAQARQSAAAGVVSAAEHPAVAPGRPRAPAAILGA
                     EGWHRSARSGTLRAIIFGVSDGLVSNLALVMGIAGASRQAEFVLLAGIAGLLAGAFSM
                     AAGEYISMQSQRELFERQIELERAELEAMPEEEQRELAALYMAKGFPRAEADKIAARM
                     FEDPATALDTLVREELGLDPDELGSPWGAAFGSFLAFALGAVIPVIPYVFLEGTTAFI
                     VSIVASLIALFLVGAGVSLLTGRGTLFSGLRQLAIGAGAAAVTFAVGTVIGVSVGG"
     gene            1026..1316
                     /locus_tag="AC162A_02630"
     CDS             1026..1316
                     /locus_tag="AC162A_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5009761.1"
                     /translation="MRAEGLDPGTWSNGSNDRYAAHSHGYDKVLVCTAGSIRFGLPDT
                     GDAVGLAAGDRLELPAGTAHDALVGPGGVTCLEAHLPAGRLAALRHLPAGEW"
     gene            complement(1381..>1606)
                     /locus_tag="AC162A_02635"
     CDS             complement(1381..>1606)
                     /locus_tag="AC162A_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009762.1"
                     /translation="GSLGRRPRRIGRRDTGNRSAAVPGTERCTTMVIVAAFALLALFS
                     IISIVLSAEDPERPSENRDNRYMWTVLGRR"
BASE COUNT          204 a          549 c          610 g          243 t
ORIGIN      
        1 atgcgcgtgc gcgtgatcat cctggtggcc cgcctgttcg ggacgcgctc cgtctccgac
       61 ctcgttcagg cgctcgaggg cgacgaggag gcgatctacg acacccaggg cgacagcccg
      121 gatatcgagg cgatcgccgc cgacgagcga gagcacgcgg agatctggcg gcggctcgac
      181 ggcgaggagg ggaccgacgg cagggcgcag gcccgtcagt cggcagccgc cggcgtcgtc
      241 agcgcggcgg agcatccggc cgttgcgccg gggcggccgc gcgcgcccgc ggcgatcctg
      301 ggcgccgagg gctggcatcg cagcgcgcgc tccgggacgc tgcgggcgat catcttcggc
      361 gtctcggacg ggctggtctc gaacctggcg ctggtcatgg gcatcgccgg cgcgtcgagg
      421 caggcggagt tcgtgctcct cgccgggatc gcgggccttt tggcgggcgc gttctcgatg
      481 gcggccggcg agtacatctc gatgcagagc cagcgcgagc tgttcgagcg ccagatcgag
      541 ctggagcgcg ccgagctcga ggcgatgccc gaggaggagc agcgcgagct cgccgccctg
      601 tacatggcca agggcttccc gcgcgcggag gccgacaaga tcgccgcacg catgttcgag
      661 gacccggcga cggcgctcga cacgctcgtt cgggaggagc tcggcctcga ccccgacgag
      721 ctggggtcgc cgtggggcgc ggcgttcggc tcgttcctcg cgttcgcgct cggcgccgtc
      781 atcccggtca tcccgtacgt gttcctcgag ggaacgacgg cgttcatcgt gagcatcgtg
      841 gcgagcctga tcgctctgtt cctcgtcggt gcgggcgtga gcctcctcac cggccgcggc
      901 accctgttct ccgggctgcg ccagctcgcg atcggtgcgg gtgcggccgc cgtgacgttc
      961 gccgtcggga cggtgatcgg cgtgagcgtc gggggctgac ggtggcgccg acgatcgccg
     1021 accgcttgcg cgccgagggc ctggatcccg gcacctggtc gaacggttcg aacgaccggt
     1081 acgccgccca cagccacggc tacgacaagg tcctggtgtg cacggcgggc tcgatccggt
     1141 ttggtctgcc cgacacgggc gacgcggttg gtctcgcggc gggcgaccgt ctcgagctcc
     1201 cggcgggtac cgcgcacgac gcgctcgtcg ggccgggcgg cgtcacctgc ctcgaagccc
     1261 acctcccggc gggacggctc gcggcgctgc ggcacctgcc ggcgggggag tggtgacgcc
     1321 gcgaatcacg aacgccccgg ccggagccgg ggcgtcaatg ggataggctg tagttcgtgc
     1381 ctagcggcgg ccgagcacgg tccacatgta ccggttgtct cggttttcgc tcgggcgctc
     1441 cggatcctcg gcgctcaaga cgatgctgat gatcgagaac agcgcgagga gcgcgaacgc
     1501 ggcaacgatg accatcgtcg tgcacctttc tgtgcctgga actgccgcgg accggttgcc
     1561 cgtgtcccgc cggccgatcc ggcggggtcg acgtcccaat gaccct
//
LOCUS       DBNHXO010000126         1615 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1220909, whole genome shotgun sequence.
ACCESSION   DBNHXO010000126 DBNHXO010000000
VERSION     DBNHXO010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1220909"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="AC162A_02640"
     CDS             <1..823
                     /locus_tag="AC162A_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012609.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HIR5009763.1"
                     /translation="EPGAYLDELAKRIGRPAVSATRRRERLRAEADARADSVRERLSG
                     QPDELAAFERLLALARDIGPLTEGHNYWIDRMAQSRLRQLATRVGRRLAAEGTIGTPD
                     DVFFLEHRDITTALATPSDLKSLVAERRAAFAQQRAIRPPAVVGKPPEATTTVDRFEG
                     ARIESDVADELRGTGASGGIVRGPARVALSPADFGRIQPGDIIVCPSSNPSWVPVFVI
                     AGGLVTNTGGVLSHAAVVAREFGLPAVVGIAGATDQIADGREVEIDGTRGIVRML"
     gene            820..>1615
                     /locus_tag="AC162A_02645"
     CDS             820..>1615
                     /locus_tag="AC162A_02645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5009764.1"
                     /translation="MTGDRRERTLSALMLGVASGAILVPLNSTMLAVALPGIMDEFGL
                     GANQVSSVVTLYLGAVAVALPVSGSLGDRFGHRLTFIAGVLAFGAASLLAAISTSFAV
                     LELARVLQAISGAFVSTGSASIIRDIAPAGRRGEAFGLFDLLVSVSAAVGPFVGGVLV
                     GAFGWRSMFYLAVPIAILAAAAVAFMLAPRRNANVQVRPLDVPGLVVLAALIVAFLVA
                     LRTDATDVRIGATLGTLVLLVAFVAVELRTEHPAVDPRLFRSRSFSA"
BASE COUNT          186 a          614 c          557 g          258 t
ORIGIN      
        1 ggagcccggc gcctacctcg acgagctcgc caagcgcatc ggccggccgg cggtgtcggc
       61 gacccgtcgg cgcgaacggc tgcgggccga ggccgacgcc cgcgctgaca gcgtccgcga
      121 acggctcagc ggccagcccg acgagctcgc ggcgttcgag cggctcctgg ccctcgcccg
      181 ggacatcggt cccctgacgg agggtcacaa ctactggatc gaccggatgg cccagtcgcg
      241 ccttcgtcag ctcgcgacgc gagtcggccg caggctcgcc gcagagggga ccatcgggac
      301 ccccgacgac gtgttcttcc tcgagcaccg cgacatcacc accgcgcttg cgacaccgag
      361 cgatctcaaa tccctggtcg cggagcgcag ggccgccttc gcgcagcagc gggcgatccg
      421 tccaccggcc gtcgtcggca agcctccgga ggcaacgacg accgtcgaca ggttcgaagg
      481 agcgaggatc gagtcggacg tggccgacga gctccggggc acaggcgcct cgggcggcat
      541 cgtgcgcggt cccgcgcgcg tcgcgctgtc gcccgccgac ttcggccgga tccagcccgg
      601 cgacatcatc gtctgcccat cgtcgaaccc gtcctgggtc cccgtgttcg tgatcgccgg
      661 cggcttggtg acgaacaccg gcggcgtcct gtcgcatgcg gccgtcgtcg cgcgcgagtt
      721 cgggctgcca gcggtcgtcg gcatcgccgg cgccacggac cagatcgccg acgggcgcga
      781 ggtcgagatc gatggcaccc gcggcatcgt ccggatgctg tgacgggaga ccgccgggaa
      841 cgaacgctct ccgcgctgat gctgggggtg gcgagtggcg ccatcctcgt cccgctcaac
      901 tcgacgatgc tggccgtcgc cctgccgggg atcatggacg agttcggcct cggcgcgaac
      961 caggtgtcct ccgtcgtcac gctgtacctc ggggcggtcg ccgtcgccct gccggtcagc
     1021 ggctcgctgg gcgaccgctt cggtcatcgg ctgacgttca tcgccggcgt gctggcgttc
     1081 ggcgccgcct cgctgctggc ggcgatctcc acgtcgttcg ctgtcctcga gctcgcgcgc
     1141 gtcctccagg cgatcagcgg cgcgttcgtc tcgaccggct cggcctcgat catccgcgac
     1201 atcgccccgg ccggacggcg gggcgaggcg ttcggcctgt tcgacctcct ggtctcggtc
     1261 agcgccgcgg tcggcccatt cgtcggcggc gttctcgtcg gcgcgttcgg ctggcgctcg
     1321 atgttctatc tcgcggtccc gatcgcgatc ctggccgcgg cggccgtcgc cttcatgctg
     1381 gcgccccggc gcaacgccaa cgtccaggta cgacccctcg atgtcccggg cctggtcgtc
     1441 ctcgccgcgc tgatcgtcgc gttcctcgtc gccctccgca ccgacgccac cgacgtccgc
     1501 atcggtgcca cgctcgggac gctcgtcctg ctggtggcat tcgtggcggt cgagctgcgc
     1561 accgagcacc ccgccgtcga cccacggctg ttccgttcgc gctccttctc ggccg
//
LOCUS       DBNHXO010000127         2177 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3998495, whole genome shotgun sequence.
ACCESSION   DBNHXO010000127 DBNHXO010000000
VERSION     DBNHXO010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3998495"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /gene="rplP"
                     /locus_tag="AC162A_02650"
     CDS             <1..224
                     /gene="rplP"
                     /locus_tag="AC162A_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR5009765.1"
                     /translation="PATKKPAETRMGSGKGAPDHWVAVVRPGRILFEMAGVEHEAAVE
                     AMRLASHKLPVATRVVTRDGGKEVTDGDR"
     gene            211..417
                     /gene="rpmC"
                     /locus_tag="AC162A_02655"
     CDS             211..417
                     /gene="rpmC"
                     /locus_tag="AC162A_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR5009766.1"
                     /translation="MEIGEVRQLSDRELDDRLREAKQDLWQARFALSTRQLKDYSQIP
                     ATRRTIARILTVQRERATAPRSAE"
     gene            428..700
                     /gene="rpsQ"
                     /locus_tag="AC162A_02660"
                     /pseudo
     CDS             428..700
                     /gene="rpsQ"
                     /locus_tag="AC162A_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
     gene            826..1194
                     /gene="rplN"
                     /locus_tag="AC162A_02665"
     CDS             826..1194
                     /gene="rplN"
                     /locus_tag="AC162A_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR5009767.1"
                     /translation="MIQSQTRLRVADNTGARTIQCIRVMGRSRKTTAGIGDVIVASVK
                     TAIPNAPVKKGDVVRAVIVRTAKEIGRPDGSYIRFDENAAVLINNQGNPRGTRIFGPV
                     ARELRERNYMKIVSLAPEVL"
     gene            1194..1634
                     /gene="rplX"
                     /locus_tag="AC162A_02670"
     CDS             1194..1634
                     /gene="rplX"
                     /locus_tag="AC162A_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR5009768.1"
                     /translation="MTVRSIERQTKVPEIRKGDTVVVLAGKDSGKRGKVTHVIRRDAS
                     PTPVRSGYRRTSLKGGVFVVVDGLNIARKHTKPRQRSSGGMGSMPTIDPGGILDHALP
                     LPVSRVMVVCGQCSEPTRIAHRTLDNGSRVRVCKHCGEPLEVQS"
     gene            1631..2173
                     /gene="rplE"
                     /locus_tag="AC162A_02675"
     CDS             1631..2173
                     /gene="rplE"
                     /locus_tag="AC162A_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR5009769.1"
                     /translation="MSGQLRERYLTEIVPALEKQFGYENPMRVPRLEKIVLNIGLGEA
                     LANAKAVDAAVGDIQLITGQKPIVTRAKRSIAQFRVRTGNPIGVKVTLRGERMWDFLE
                     RMTRLALPRIRDFRGVPGRSFDGRGNYSLGLREQLAFPEIDYDKVDRLRGLEISIVTT
                     AKTDEESKRLLELLGMPFAG"
BASE COUNT          378 a          785 c          738 g          276 t
ORIGIN      
        1 agcccgcgac caagaagccc gccgagacgc ggatgggttc gggcaagggc gcccccgatc
       61 actgggtcgc cgtcgtgcga cccggccgga tcctgttcga gatggccggc gtcgagcacg
      121 aggccgcggt cgaggccatg cgcctcgcat cgcacaagct tccggttgcg acccgcgtcg
      181 tgacgcgcga cggcggcaag gaggtcaccg atggagatcg gtgaggtccg acagctctct
      241 gatcgggagc tcgacgaccg ccttcgggag gccaagcagg acctgtggca ggcgcgcttc
      301 gccctctcga cacgacagct caaggactac agccagatcc cggcgacgcg acgcacgatc
      361 gctcggatcc tcaccgtcca gcgcgagcgg gcaaccgccc cgcgctcggc cgagtaggag
      421 cgccgggatg actaccgcag gagccaccaa ccccgtgaag ggccagcgaa agaccaaggt
      481 cggccgcgtg gtcagcgaca agatggacaa gacgatcgtc gtgtccgtcg agcgcctgac
      541 gcgccacccc ctgtacaagc gcgtcatgaa ggcgtcgacg aagttcgccg cccacgacga
      601 gaccaacgac gcccggatcg gcgacaccgt cctcatcgag gagtcgcggc cgctgtccgc
      661 caccaagcgc tggcgcctgg tgtcggtcct ctcccgcgcg ggcgaggagc aggccggcgt
      721 gtcgctcgtg gccgaggagg ccgagaccac cgaggccatc catggcgccg cccacccggg
      781 ccgccggacc gaggcccagg agccggccgc cgaggaggcc gagtcgtgat ccagagtcag
      841 acgcgactgc gcgtcgccga caacaccggc gcgcgcacga tccagtgcat ccgggtcatg
      901 ggccgctcgc gcaagaccac ggccggcatc ggcgacgtga tcgtcgccag cgtcaagacc
      961 gccatcccca acgccccggt caagaagggc gatgtcgtgc gcgcggtcat cgtgcgcacc
     1021 gcgaaggaga tcggccgccc ggacgggagc tacatccggt tcgacgagaa cgcggccgtg
     1081 ctcatcaaca accagggcaa cccgcgcggc acccgcatct tcggaccggt cgcccgcgag
     1141 ctgcgcgaac gcaactacat gaagatcgtg tccctcgcgc cggaggtgct gtgatgaccg
     1201 tcaggtccat cgagcgccag accaaggtgc ccgagatccg caagggcgac accgtggtgg
     1261 tgctcgccgg caaggactcc ggcaagcggg gcaaggtcac ccacgtcatc cggcgcgacg
     1321 cctcgcccac ccccgtgcgc tccggctacc gccggacgtc gctcaagggc ggcgtgttcg
     1381 tggtcgtcga cgggctcaac atcgcccgca agcacaccaa gccccgccag cgctcgtccg
     1441 gcggcatggg ctcgatgccc accatcgatc ccggcggcat cctcgatcac gccctgccgc
     1501 tcccggtcag ccgcgtgatg gttgtctgcg gccagtgcag cgagccgacc cgcatcgccc
     1561 accggacgct cgacaacggg tcgcgcgtcc gcgtgtgcaa gcactgcggc gagcccctgg
     1621 aggtgcagtc gtgagcggcc agctcaggga gcgctacctg acggagatcg tgccggcgct
     1681 ggagaagcag ttcggctacg agaacccgat gcgcgtgccg cgcctcgaga agatcgtcct
     1741 caacatcggc ctcggcgagg ccctggcgaa cgccaaggcc gtcgacgcgg cggtgggcga
     1801 catccagctc atcacgggcc agaagccgat cgtcacccgc gccaagcggt cgatcgcgca
     1861 gttccgggtc cgcacgggca acccgatcgg cgtgaaggtc acgctccgcg gcgagcggat
     1921 gtgggacttc ctcgagcgca tgacgcgcct cgccctgccg cgcatccgcg acttccgcgg
     1981 cgtgcccggg cggtcgttcg acggccgtgg caactactcg ctcggtctcc gggagcagct
     2041 tgcattccca gagatcgact acgacaaggt ggaccgtctc cgcgggctgg agatcagcat
     2101 cgtgacgacg gcgaagaccg acgaggagtc gaagcgtctg ctcgaactcc tcggcatgcc
     2161 cttcgccggc taggcgc
//
LOCUS       DBNHXO010000128         2084 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1816239, whole genome shotgun sequence.
ACCESSION   DBNHXO010000128 DBNHXO010000000
VERSION     DBNHXO010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1816239"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1811)
                     /locus_tag="AC162A_02680"
     CDS             complement(<1..1811)
                     /locus_tag="AC162A_02680"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR5009770.1"
                     /translation="MLRQPFSFDALGRFAVRRRWWIVAAWAAILLAAIPLAPQAPSAL
                     SAGGFILDDLESARAKQLLQDELGVEASAFVLVYTSDSLRAGTPDWQAAVDAATRDVA
                     GAPHVTRVLPHALAPSQVSRDGHTAYDIVFLDLPPDDSPDAIPGVLERLHAVPGLTVR
                     TGGGPAFYGDVQSVTESDLRRSELISLPLAALALILVFGSLVAAGVPLAVGGAAVLVA
                     LAGIFVVASVIPMSIFVLNLATLLGLGLGVDYSLLMTSRFREELARRDGEDRVAEAVR
                     ITVATAGRAVFFSGLTVLLGLLGLVLFEFMILRSVGIAGAIVVGLAVASALTLLPAIL
                     AIVGTRIDSLAVRRVTADPGVDGPWARLARKVMRHPVAVLVPTLSLLLILGVPFLHVR
                     FNAPDSTILPPSVPSREAYDILGREFGEGAFAPLALAIRTTGDATTPDNLTKLYDYSR
                     RLAADPRVTHVVGLVDVDPRITLPQYQLLYGSPGGPPDRFVATALAATTKGDLTAFTI
                     YTPYGPNRDEPRALVHELRDPTSALAPPAGISVLVGGGAADVDDVVSRVWSDFPRTAL
                     FIVVTTFLVLFALLRSVVLPIKALVMNTLSIVASFGAL"
BASE COUNT          351 a          729 c          764 g          240 t
ORIGIN      
        1 agcgccccga agctggccac gatcgagagg gtgttcatga ccagcgcctt gatcggcagg
       61 acgaccgacc gcaggagcgc gaacaggacg aggaacgtgg tcacgacgat gaacagcgcc
      121 gtgcgcggga agtcggacca gacgcgcgac acgacgtcgt cgacgtccgc cgcaccgccg
      181 cccaccagga ccgagatccc ggccggcggt gcgagcgcac tcgtcgggtc gcgcagctca
      241 tggaccagcg cgcggggctc gtcgcggttc ggcccatagg gcgtgtagat cgtgaatgcc
      301 gtcagatcgc ccttggtcgt cgccgccagc gccgtcgcca cgaaccggtc gggcggccct
      361 cccggcgacc cgtagaggag ctggtactgc gggagcgtga tccgggggtc gacgtcgacg
      421 aggccgacga catgggtcac ccgcgggtcc gccgccaagc gccgcgagta gtcgtagagc
      481 ttggtgaggt tgtcgggagt cgtcgcgtcg ccggtcgtgc ggatcgcgag cgcgagcggc
      541 gcgaaggcgc cctcgccgaa ctcgcggccg aggatgtcgt acgcctcgcg cgacgggacg
      601 gacggcggca ggatcgtcga gtccggggcg ttgaaccgga cgtgcaggaa cggcacgccg
      661 aggatcagca gcagcgagag ggttgggacg agcaccgcga ccgggtggcg catcaccttg
      721 cgcgcgagcc tcgcccaggg gccatccacg cccgggtccg cggtgacgcg gcgaacggcg
      781 agcgaatcga tgcgcgtgcc gacgatcgcg aggatcgcgg gcaggagcgt gagcgccgag
      841 gccaccgcga gcccgaccac gatcgcgccc gcgatcccca ccgagcgcag gatcatgaac
      901 tcgaacagca cgaggcctag caggcccagc agcacggtca gccccgagaa gaacacggcc
      961 cgcccggcgg tcgcgacggt gatccggacg gcctcggcga cccggtcctc gccgtcgcgc
     1021 cgcgccagct cctcgcggaa gcggctcgtc atcagcagcg agtagtcgac gccgagaccc
     1081 aggcccagca gcgtcgcgag gttgagcacg aagatgctca tcgggatgac ggacgcgacg
     1141 acgaagatcc cggccagcgc caccagcact gcggccccgc caaccgccag cggcacgccg
     1201 gcggccacca gcgacccgaa cacgaggatc agcgcgagag ccgcgagcgg cagcgagatc
     1261 agctcggacc gccgcaggtc cgactccgta accgactgga cgtcgccgta gaacgccggc
     1321 ccgccgcccg tccggacggt gaggccgggc accgcgtgga gccgctcgag gacgccgggg
     1381 atcgcatcgg gcgagtcgtc gggcggcagg tcgaggaaca cgatgtcata cgccgtgtgg
     1441 ccgtcgcgcg agacctggct cggggcgagg gcgtgcggca ggacgcgggt cacgtgcggg
     1501 gcgccggcga cgtcgcgcgt cgccgcgtcg acggccgcct gccaatcggg ggtgcctgct
     1561 ctgagcgagt ccgaggtgta gacgaggacg aacgccgacg cctcgacgcc cagctcgtcc
     1621 tggaggagct gcttcgcccg cgcggactcg aggtcgtcga ggatgaaccc gccggcggag
     1681 agcgccgagg gcgcctgcgg cgcgagcggg atcgccgcca gcaggatcgc cgcccacgcc
     1741 gcgacgatcc accaccggcg gcggactgcg aaccggccca gggcgtcgaa gctgaacggc
     1801 tgtcgtaaca tgcggctcac aagtagcatg ttacacgaga cccgggtcgg acgccgccga
     1861 ccggacgtcc cattctgcta ggatcgagcg gccaatcgcc ttggcccagc gtcgcgaacc
     1921 gccatccccg accgaggctc ccgcgtgacg atcttcacga tctggttcca gaacttcccc
     1981 aacaacttcg tgatcctgct cgccgcgtcc gcgatcgcgg cggccttcgt cctgatcttc
     2041 gccaagtccg cgatggtcgc cgagggtggc cgccagggct gggt
//
LOCUS       DBNHXO010000129         3673 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3801025, whole genome shotgun sequence.
ACCESSION   DBNHXO010000129 DBNHXO010000000
VERSION     DBNHXO010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3801025"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="AC162A_02685"
     CDS             <1..513
                     /locus_tag="AC162A_02685"
                     /inference="COORDINATES: protein motif:HMM:NF018434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5009771.1"
                     /translation="VTMGTMPLASQAVLPWVADAFSGLDAEVVVALGPATDPSLLGRP
                     PDNVRLVPYVPMSRLLPTCAAVVFHAGSGTMLAALSAGVPLVLLPVGADQPDNAERCA
                     AAGVGIVVGPEVRDAASVRAAVERVLGSASFSEASRRVRLEIAGMPEPATVLLEVERL
                     ADSGDRPVSG"
     gene            563..1636
                     /gene="ruvB"
                     /locus_tag="AC162A_02690"
     CDS             563..1636
                     /gene="ruvB"
                     /locus_tag="AC162A_02690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR5009772.1"
                     /translation="MTDDGLSLLAAGATEPDELVVEGSLRPRTLDEYIGQREVKASLD
                     VLLQAAKGRGEAADHVLLYGPPGLGKTTLATIIARELGVNVRYTSGPAVERAGDLAAI
                     LTALDERDVLFIDEIHRLNRAVEEILYPAMEDFALDVMIGKGPSARSLRLSLKPFTVV
                     GATTRAGRISGPLRDRFGMTYRLDFYGEDELTAIVRRSAGILGVEVDADAARLIAKRG
                     RGTPRIVNRLLRRVRDHAEVHGDGRIHEQAVAEAMVVLDIDDLGLDTTDRKLLAAIVQ
                     KFGSGPVGVQALAAVLAEEVETIEDVYEPFLLRLGFLDRTPQGRIATEGARQHLAALG
                     YEIPPPRRAEPDMPAFWDDVPST"
     gene            1639..1863
                     /locus_tag="AC162A_02695"
     CDS             1639..1863
                     /locus_tag="AC162A_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HIR5009773.1"
                     /translation="MDSLEPFGRVLIVIGLVIAAAGAVMAFGSRIPVLGHLPGDITIK
                     GDNVTIFIPLGTMLVVSIVASVVLSLLARR"
     gene            1865..3025
                     /gene="tgt"
                     /locus_tag="AC162A_02700"
     CDS             1865..3025
                     /gene="tgt"
                     /locus_tag="AC162A_02700"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR5009774.1"
                     /translation="MTGAATFEVLAPPPGDGSTRARLGRLTLPHGVVETPQFMPVGTN
                     ATVKALHPDEVADAGATILLANTYHLYLRPGHERIERLGGLHMFMGWDRPILTDSGGF
                     QVVSMGDLRVIDDEGVTFKSHLDGSTHRFTPEHSIAVQQALGPDIAVAFDQPVFPTSP
                     RAVVEDAMHRTHRWAERSLEAHTRTDQALFGIVQGGLDPALREESARFIAALPFDGIN
                     IGGLAGDETPEERNRTLDETVPYLDGDPRPRYLMGLGSPLDLLDAVHRGIDLFDSVLP
                     ARVARNGQLWVPGGRLNLRNEQFRDDPRPVQDDCPCPLCTRFSRAYLSHLFRAKEMLA
                     YRLATCHNLTFTLDFMVRIRAALRAGTFPVETPRLRSRAVRMSGGEGAAKPA"
     gene            3066..3227
                     /locus_tag="AC162A_02705"
     CDS             3066..3227
                     /locus_tag="AC162A_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009775.1"
                     /translation="MGSRDRPHKEVKKKPKDKSSQPSLSPLSEPPQQVEVIRKQRKPR
                     WDEPESSEG"
     gene            3297..>3673
                     /locus_tag="AC162A_02710"
     CDS             3297..>3673
                     /locus_tag="AC162A_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HIR5009776.1"
                     /translation="MTITGKDLDARNGGTEKAGGGTEKAVAERGRAVDAAIVAIEKQF
                     GRGSIMKLGSRERQAVDSIPTGSIALDLALGVGGIPRGRITEVFGPESSGKTTLCQHV
                     IAEAQRYGGIAAFIDVEHALDPA"
BASE COUNT          536 a         1318 c         1295 g          524 t
ORIGIN      
        1 gtgacgatgg gcacgatgcc gctcgcgagc caggccgtgc tgccctgggt cgccgacgcg
       61 ttctccggcc tcgacgcgga ggtggtcgtg gcgctcggtc ccgcgacgga cccatccctg
      121 ctcggcaggc cccccgacaa cgtccggctc gtgccctacg tgccgatgtc ccgcctcctt
      181 ccgacctgtg cggcagtggt cttccatgcc gggtccggca cgatgcttgc ggcgctctcg
      241 gccggcgtcc cgctcgtgct cctgccggtg ggcgccgacc agccggacaa cgccgagcgc
      301 tgcgcggccg cgggcgtggg gatcgtcgtc ggccccgagg tgcgtgacgc ggcgtccgtc
      361 cgcgcggcgg tggagcgcgt gctcgggagc gcctccttca gcgaggcgtc gcggcgcgtg
      421 cggctggaga tcgccgggat gccagagccc gcgacggtcc tcctcgaggt cgaacgactc
      481 gcggattcgg gcgatcggcc ggtgtccgga tgaaatcgaa catgcgtacc aaaccgaccc
      541 ggaacctgct agactcccgg cgatgaccga cgacggcctc agcctcctcg cggccggcgc
      601 caccgaaccc gacgaactcg tcgtcgaggg gtcgctgcgc ccgcggacgc tcgacgaata
      661 catcggccaa cgggaggtca aggcgagcct cgacgtcctg ctccaggcgg ccaaggggag
      721 gggagaggcg gccgatcatg tcctcctcta cggcccgccc ggtctgggca agacgacgct
      781 cgccacgatc atcgcccgag agctcggcgt gaacgtccgt tacacgagcg gcccggcggt
      841 cgagcgagcc ggagacctgg ccgcgatcct gaccgcactc gatgagcggg acgtcctgtt
      901 catcgacgag atccaccggc tgaaccgcgc ggtcgaagag atcctgtacc cggcgatgga
      961 ggacttcgcg ctcgacgtga tgatcggcaa gggcccgagc gcgcggagcc tgcgcctgag
     1021 cctcaagccg ttcaccgtcg tcggtgcgac gacccgggcc ggccggatca gcgggccgct
     1081 ccgcgatcgc ttcggcatga cctaccgcct cgacttctac ggcgaggacg agctgacggc
     1141 gatcgttcgg cggtcggcgg ggatcctggg ggtcgaggtg gacgccgatg ccgcgcggct
     1201 gatcgccaag cgtggtcgag gcacgccgcg catcgtcaac cggctgctcc gccgggtccg
     1261 cgaccatgcc gaggtccacg gcgatggccg gatccacgag caggccgtcg cggaggcgat
     1321 ggtcgtgctc gacatcgacg acctgggcct cgacaccacg gaccgcaagc tgctggccgc
     1381 gatcgtacag aagttcgggt cggggccggt cggcgtccag gccctcgccg ccgtgctcgc
     1441 tgaggaggtc gagaccatcg aggacgtgta cgagccgttc ctgctccggc tcggcttcct
     1501 ggaccgcacg ccacagggcc ggatcgcgac ggagggcgcc cggcagcacc tcgcggcgct
     1561 gggctacgag atcccgccgc cgcggcgcgc cgagcccgac atgccggcgt tctgggacga
     1621 cgttccatcg acctgaccgt ggacagcctc gagccgttcg gccgcgtcct gatcgtgatc
     1681 ggtctcgtca tcgccgccgc cggggccgtg atggcgttcg gatcgcggat cccggtgctg
     1741 ggtcacctgc cgggcgacat cacgatcaag ggcgacaacg tgacgatctt catcccgctg
     1801 ggcaccatgc tcgtggtctc gatcgtggcc tcggtcgtcc tcagcctcct ggcgcggcgc
     1861 tgacgtgacc ggcgcggcaa ccttcgaggt gctcgcaccg ccgcccggcg acggctcgac
     1921 ccgggcgcga ctcggccgcc tcacgctccc gcatggcgtc gtcgagacgc cgcagttcat
     1981 gccggtgggg acgaacgcga cggtcaaggc gctccacccc gacgaggtcg cggacgccgg
     2041 cgcgacgatc ctcctcgcca acacgtacca cctgtacctg cgcccgggcc acgagcggat
     2101 cgagcggctc ggcgggctgc acatgttcat gggctgggac cggccgatcc tcaccgactc
     2161 cggcggtttc caggtcgtct cgatgggcga cctgcgggtg atcgacgacg agggggtgac
     2221 gttcaagagc catctcgatg ggtcgaccca ccggtttacg cccgagcact cgatcgcggt
     2281 ccagcaggcg ctcggacccg acatcgccgt cgccttcgac cagccggtgt tcccgacctc
     2341 gccgcgagcg gtcgtcgagg acgcgatgca ccggacgcac cgctgggccg agcgctcgct
     2401 cgaggcccat acgcggaccg atcaggcgct gttcgggatc gtccagggcg gcctggaccc
     2461 cgcgctgcgt gaggagtcgg cccggttcat cgccgcgctg ccgttcgacg gcatcaacat
     2521 cgggggcctc gctggcgacg agacgcccga ggagcgcaac cgcacgctgg acgagaccgt
     2581 cccgtacctc gacggcgacc cgcggccgcg gtacctgatg ggcctcggtt cgccgctcga
     2641 cctcctcgac gccgtccacc ggggcatcga cctgttcgat tccgtgctcc cggcgcgggt
     2701 cgcgcgcaac ggacagctgt gggtgccggg cgggcgtctg aacctgcgca acgagcagtt
     2761 ccgggacgac ccgcggccgg tccaggacga ctgtccgtgc ccgctgtgca cccggttttc
     2821 gcgggcgtat ctgagccatc tgttccgggc caaggagatg ctcgcgtacc gcctcgcgac
     2881 ttgtcacaac ctcaccttca ccctagactt catggtcagg atccgggctg cgctgcgcgc
     2941 cggaaccttc cccgtcgaga ccccccggct gcgctctcgc gccgtgcgga tgagtggcgg
     3001 tgaaggcgcc gcgaagccgg cgtgaagtcc ctgtcggtat cgtcagttgt gcgtggaggc
     3061 gcgaaatggg atcacgggat cgcccccaca aggaagtcaa gaagaagccc aaggacaagt
     3121 cctcccagcc gagcctgtcg ccgctctccg agcctcccca gcaggtggag gtcatccgga
     3181 agcagcgcaa gccgcgctgg gacgaacccg agtccagcga gggctgagcc gccgggcgga
     3241 cggggaccgg gtcccgctcc gtcgcgtcgg cacaagccgc acggagcgag gatgggatga
     3301 cgatcaccgg caaggacctg gacgcccgca acggcggcac cgagaaggcg ggcggcggca
     3361 ccgagaaggc cgtcgcggag cgcgggcgag cggtcgacgc cgcgatcgtc gcgatcgaga
     3421 agcaattcgg ccgcggctcg atcatgaagc tgggctcgcg cgagcgccag gccgtcgact
     3481 cgatccccac gggctcgatc gccctcgacc tcgcgctcgg cgtgggcggg atcccgcgcg
     3541 gccggatcac cgaggtcttc ggccccgagt cctcgggcaa gaccacgctc tgccagcacg
     3601 tgatcgccga ggcgcagcgc tacggcggca tcgccgcgtt catcgacgtg gagcacgcgc
     3661 tcgacccggc cta
//
LOCUS       DBNHXO010000130         7272 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5189610, whole genome shotgun sequence.
ACCESSION   DBNHXO010000130 DBNHXO010000000
VERSION     DBNHXO010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7272)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5189610"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="AC162A_02715"
     CDS             complement(<1..1395)
                     /locus_tag="AC162A_02715"
                     /inference="COORDINATES: protein motif:HMM:NF037144.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5009777.1"
                     /translation="MATTSLLLAGALFAPSVALAWGIPPIAFPDNVTTNEDTAKSGNV
                     LDNDWNISDSAMTVTGFVALNPTVGVIMIATNGNYTFVPAANYHGNASTTYTAHNDEG
                     NDTGTINITVSSVNDNPVANDDTITATEDTTTGNVSATMRSNDTDVDGDSLTVTAVSN
                     STGGTATRTGGTGGTVTFAPTANLCGVGAGGYDYTLSDGHGGTDTGHVTVNITCVNDG
                     PTANDDSVSVTEDTATDVTASILTNDTDPDGDTLVVTAVSNEAAGTVSLVAGVVTFTP
                     TADLCGAGIGGFDYTADDGAGGSDSAHVIVSITCVNDDPVADDDTVSGTEDLDVTVTE
                     FDMVGNDADVDSGTLTVAAVSNETGGSASLDAGEVTFTPDEDLCGVGAAGYDYTVEDV
                     DSGSDTGHVTIDLPCITDDPVAVGDTLSGTEDTDLVVDAADLRANDSDVDGDDLTVTS
                     VGTAGGGSVDLDGTT"
     gene            complement(2084..4141)
                     /locus_tag="AC162A_02720"
     CDS             complement(2084..4141)
                     /locus_tag="AC162A_02720"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004943897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5009778.1"
                     /translation="MSGPTNPGTNGSNPPPVRAVGNSVPGPEGPGAAGPRRPMGGPGG
                     GPGFMGGLTTPAAKSKDFKASFRRLLGELRPEGSKVLVVLALAVGSVFFAILGPKILG
                     AATNLIFAGAVGNQLPPGATIQQLVDQLNAAGETNQAQLLANLAGVVPGQGIDFAALA
                     QILMLAAFVYIVSSVFAWAQSYIMAGVTQRTVYRLRRRVDEKLGRLPLAYFDGQSRGD
                     ILSRVTNDIDNISQSLQQSLTQLITSVLTVIGVLIMMLTISPLLALISLLAVPASLVV
                     TVLIAKRSQKQFVAQWGTTGQLNGHIEEMHTGHAIVKVFGRQPEAIEAFDIQNESLYQ
                     ASYKAQFISGLIQPAMTFISNINYVLICVIGGIQVANGQMSLGDVQAFIQYSRQFTFP
                     IIQAASIANVLQSAVASAERVFELLDEPEEIADPVAPRLLPVPATGRVAFEDVAFRYV
                     ADKPLIEDMDLVVEPGQTVAIVGPTGAGKTTLVNLLMRFYDVDRGRITVDGIDTRELA
                     RDDLRRTFGMVLQDAWLFKGTIRENVAYGAKGEPDDARFREAVEAAHVNHFVRTLPDG
                     YDTVLDDDATNVSAGEKQLLTIARAFLADPPVLILDEATSSVDTRTEVLIQKAMSRLM
                     RNRTSFVIAHRLSTIRDADVILVMNEGRIIEQGTHDQLMAKGGFYHELYASQFEEALD
                     SVA"
     gene            complement(4138..5808)
                     /locus_tag="AC162A_02725"
     CDS             complement(4138..5808)
                     /locus_tag="AC162A_02725"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5009779.1"
                     /translation="MILLAIQAVANLYLPELNADIINNGVVTGDIDYILRTGGFMLLV
                     TFILGVAAVVAAYWSAKVAMGFGRDVRSQVFRKVETFSQVEVNTFGAASLITRNTNDV
                     QQVQTVLLLALTVMISAPIMVVGGLIMALRQDIPLSGVLLVIIPIMFALIGIVMYKAV
                     PLFQVMQKKVDRINLVMRETLSGVRVIRAFVRTRDEEARFDEASADLMDTGLRVNRLF
                     AITLPSLMAIMNLSTVAVLWLGAYRVNSGDMPIGNLTAFLQYISLILFSIMTAVIMFV
                     MVPRAAVSSVRIQEVLKTEPTIWDPPTPISPERTGHVEFRDVEFRYPGAEEPVLRHIS
                     FVAGPGETTAVVGSTGSGKSTLINLLPRFYDATGGTLLIDGVDVRDMDRQDLWSRIGI
                     VPQKAFLFGGTVASNLRFGDQEATDDDLWRALDIAQGRDFVAAMDQGLDSPITQGGTN
                     VSGGQRQRLAIARALVKKPDVYVFDDSFSALDFRTDARLRAALGRELGHATVIIVAQR
                     VGTILHADRIVVLDEGGIAGIGTHEELMRTCETYREIVLSQVTEEEVA"
     gene            complement(5862..6362)
                     /locus_tag="AC162A_02730"
     CDS             complement(5862..6362)
                     /locus_tag="AC162A_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR5009780.1"
                     /translation="MTPTTTNRKQLLNDLLDELTSHSPADMLRYMRRWPSGPLSLVHL
                     QVLTVLETDGPVPMGTLAESLDVSQASTTGIVDRMEQRGLVERRRDDEDRRVVRVALT
                     EDGTRLLAGVAAGRRERLGILLGELSDEELDGFLRGSRALREARMRLAPKWATPTSGP
                     TDEASR"
     gene            complement(6784..>7272)
                     /locus_tag="AC162A_02735"
     CDS             complement(6784..>7272)
                     /locus_tag="AC162A_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009781.1"
                     /translation="RMSATPTSPATIIAGEAMGRYGIAVFQATYIVVVSALFFGVSWG
                     NPVGAAAIILVFSLVSAGAAMLLGAMARNADQASSLGVFIGLALGALGGCMIPLQIMP
                     ATMQSLARLIPHSWAVTGLQSLITDGGGIDSVATNLAVLTGFAVVLLGLAAWRFRKAI
                     TG"
BASE COUNT         1076 a         2405 c         2634 g         1157 t
ORIGIN      
        1 cgtcgtgccg tcgaggtcga cgctgccgcc gccggcggtc ccgaccgagg tcacggtcag
       61 gtcgtcgcca tcgacgtcgg agtcgttggc ccggaggtcg gccgcgtcga cgacgaggtc
      121 ggtgtcctcc gtgccactga gcgtgtcgcc cacggcgacc gggtcgtcgg tgatgcaggg
      181 gagatcgatc gtgacatggc ccgtgtccga gccgctgtcg acgtcctcga ccgtgtagtc
      241 gtagccggcg gcgccgacgc cgcacaggtc ctcgtcgggt gtgaaggtga cctcgcccgc
      301 gtcgagcgag gcgctgccgc cggtctcgtt cgagaccgcg gcgaccgtga gcgtgccgct
      361 gtcgacgtcg gcgtcgttgc cgaccatgtc gaactcggtg accgtgacgt cgaggtcctc
      421 ggtgcccgag acggtgtcgt cgtcggcgac cgggtcgtcg ttgacgcagg tgatgctcac
      481 gatgacgtgc gcgctgtccg agccgcccgc accgtcgtcg gccgtgtagt cgaagccgcc
      541 gatgcccgcg ccgcagaggt ccgcggtggg cgtgaaggtg accacgcccg cgaccaggct
      601 gaccgtgccg gccgcctcgt tgctgacggc ggtgacgacg agcgtgtcgc cgtcgggatc
      661 ggtgtcgttg gtcaggatcg acgccgtgac gtcggtcgcg gtgtcctcgg tcacggagac
      721 cgagtcgtcg ttggccgtcg gcccgtcgtt gacgcaggtg atgttgaccg tgacgtgccc
      781 cgtgtcggtg ccgccgtggc cgtcggagag cgtgtagtcg tagccgccgg cgccgacgcc
      841 gcacaggttc gcggtcgggg cgaacgtgac ggtgccgccc gtgccgccgg tgcgggtggc
      901 ggtgccgccg gtcgagttgg agaccgcggt gaccgtgagg ctgtcaccgt cgacgtccgt
      961 gtcgttcgag cgcatcgtcg ccgacacatt gccggtcgtc gtgtcctcgg tcgcggtgat
     1021 cgtgtcgtcg ttcgcgacgg ggttgtcgtt gacggacgag acggtgatgt tgatcgtccc
     1081 ggtgtcgttg ccctcgtcgt tgtgggcggt gtacgtggtc gacgcgttgc cgtgatagtt
     1141 ggccgccggc acgaacgtgt agttgccgtt cgtcgcgatc atgatcacgc cgacggtcgg
     1201 gttcagggcg acgaagcccg tgaccgtcat cgcgctgtcg gagatgttcc agtcgttgtc
     1261 gaggacgttg cccgacttgg cggtgtcctc gttcgtggtg acgttgtcgg ggaaggcgat
     1321 ggggggaatg ccccacgcca gggccacgct gggggcgaag agtgcgcccg cgagcaggag
     1381 gctggtggtt gccacaccca gccttcgcag atggagcgag ctcgttggac gtcgaatgat
     1441 cacgtcgttg gtccctcagg cgtggtgaac ccgcggcgac acgtgccgcc gggttggtca
     1501 ctccggaggg tgctggagag caccgggcgc tggtaccagc cgaaagtccc ttggccggtg
     1561 gggcaatcgg tatgacggct ggccgaatgg gtcaccccgg gcggggcccc gccgggtcac
     1621 cccgcccgtg gggggtcgcg gacatgaaga cgcccccggc gatgccggcg gcgtctggtg
     1681 ctgcgtgagg ggttggagcg ctacttgagc gtgacgaagg cggcgggtcg tcgaatcgag
     1741 ccctcggggc cgatgcctcg ccggcccgtc cgccgcccgc gctccgcggt tcgagcctcg
     1801 ccttgtattc gtcacgtgaa tgccgagcgc cggtctgtcc gcgccttcgg cctcgcgagg
     1861 atcgcaccac gcacgcggcc agcccgagga gctgtcttgc atccaccgtg tgaatgttgg
     1921 gccaggtccg ggcgtcaaca gttggcccat cactcactac gtgaatgcaa ggcgcactcg
     1981 cgccccgatc cccgggcttc gcatgcaccc ggtgcatcga tggcagggtg gccccgcttc
     2041 gcattcaccc ggcgaatgca tggcctggtc gcggccggag acgctaggcg accgagtcga
     2101 gggcctcctc gaactgcgac gcgtacagct cgtggtagaa accgcccttc gccatcagct
     2161 ggtcgtgcgt gccctgctcg atgatccggc cctcgttcat gaccaggatc acgtccgcgt
     2221 cgcggatcgt ggacagccgg tgggcgatca cgaacgacgt ccggttgcgc atgagccgcg
     2281 acatcgcctt ctggatcagc acctcggtcc gcgtgtccac cgacgatgtg gcctcgtcga
     2341 ggatgaggac cggcgggtcg gcgaggaacg cccgggcgat cgtgagcagc tgcttctcgc
     2401 ccgccgacac gttggtcgcg tcgtcgtcga gcaccgtgtc gtagccgtcc ggcagcgtgc
     2461 gcacgaagtg gttcacgtgc gcggcctcga cggcctcgcg gaaccgggca tcgtcgggct
     2521 cgcccttggc gccgtacgcg acgttctcgc ggatcgtgcc cttgaacagc catgcgtcct
     2581 ggaggaccat cccgaacgtc cggcgcaggt cgtcgcgggc cagctcccgg gtgtcgatgc
     2641 cgtcgacggt gatccggccc cggtcgacgt cgtagaagcg catcagcagg ttgacgagcg
     2701 tggtcttgcc cgcgcccgtg ggaccgacga tggccacggt ctggccgggc tcgacgacca
     2761 ggtccatgtc ctcgatcagc ggcttgtccg cgacgtagcg gaacgcgacg tcctcgaacg
     2821 ccacccggcc ggtcgccggc acggggagca gccgcggggc gacggggtcg gcgatctcct
     2881 cgggctcgtc gagcagctcg aacacccgct cggccgacgc gacggccgac tgcaggacgt
     2941 tggcgatcga cgccgcctgg atgatcggga aggtgaactg gcgcgagtac tggatgaacg
     3001 cctgcacatc gcccaggctc atctggccgt tggccacctg gatgccgccg atgacgcaga
     3061 tcagcacgta gttgatgttc gagatgaacg tcatcgcggg ctggatcagc cccgagatga
     3121 actgcgcctt gtagctcgcc tggtacaggg actcgttctg gatgtcgaac gcctcgatgg
     3181 cctccggctg gcgcccgaac accttgacga tcgcgtgccc ggtgtgcatc tcctcgatgt
     3241 ggccgttgag ctggccggtc gtgccccact gggcgacgaa ctgcttctgg gaccgcttgg
     3301 cgatcaggac cgtcaccacg agggaggccg ggaccgcgag cagcgagatc agcgcgagca
     3361 acgggctgat cgtgagcatc atgatcagca cgccgatgac ggtcaggacc gacgtgatca
     3421 gctgggtcag gctctgctgg agcgactggc tgatgttgtc gatgtcgttg gtgacgcggc
     3481 tcaggatgtc gccccgcgac tggccgtcga agtaggccag cggcaggcgg ccgagcttct
     3541 cgtcgacgcg ccggcggagc cggtagacgg tccgctgggt gacgccggcc atgatgtacg
     3601 actgggccca ggcgaacacc gagctgacga tgtagacgaa ggccgccagc atcaggatct
     3661 gggccagggc ggcgaagtcg atgccctgcc cggggacgac accggccagg ttcgcgagca
     3721 gctgggcctg gttggtctcg cccgccgcgt tcagctggtc gaccagctgc tggatcgtcg
     3781 cgccgggtgg cagctggttg ccgacggcgc ccgcgaagat caggttggtc gcggcgccca
     3841 ggatcttggg gccgaggatg gcgaagaaca cgctgccgac ggcgagcgcg agcacgacga
     3901 ggaccttcga cccctccggc cgcagctcgc ccagcaggcg ccggaacgag gccttgaagt
     3961 ccttcgactt ggccgcgggc gtggtgagcc cgcccatgaa cccgggaccg ccgcccgggc
     4021 cgcccatcgg ccgcctgggt ccggcggcgc cggggccttc cggacccggg accgagttgc
     4081 cgaccgcgcg gaccgggggc ggattgctgc cgttggtgcc ggggttcgtc ggcccgctca
     4141 cgcgacctcc tcctcggtca cctgggacag gacgatctcc cggtaggtct cgcaggtccg
     4201 catcagctcc tcgtgggtgc cgatgccggc gatcccgccc tcgtcgagga cgacgatccg
     4261 gtccgcgtgg aggatcgtgc ccacgcgctg ggcgacgatg atcaccgtcg cgtggcccag
     4321 ctcccgcccc agcgcggccc gcaggcgcgc gtcggtccgg aagtcgaggg ccgagaagct
     4381 gtcgtcgaac acgtacacgt cgggcttctt gaccagcgcc cgggcgatcg cgagtcgctg
     4441 gcgctgaccg ccggacacgt tggtgccgcc ctgggtgatc gggctgtcga gcccctggtc
     4501 catcgccgcc acgaagtcgc ggccctgcgc gatgtcgagg gcgcgccaca ggtcgtcgtc
     4561 ggtcgcctcc tggtcgccga agcggaggtt gctcgcgacg gtgccgccga acaggaacgc
     4621 cttctggggc acgatgccga tccgcgacca caggtcctgg cggtccatgt cgcggacatc
     4681 gacgccgtcg atcagcagcg tgccgccggt cgcgtcgtag aagcgcggca gcaggttgat
     4741 cagcgtggac ttgccgctgc ccgtggatcc gacgacggcc gtggtctcgc cgggcccggc
     4801 cacgaacgag atgtggcgca ggaccggctc ctcggcgccc ggatagcgga actcgacgtc
     4861 gcggaactcg acgtggccgg tgcgctcggg cgagatcggg gtcggcggat cccagatcgt
     4921 cggctcggtc ttgagcacct cctggatccg gacgctcgag acggcggccc gcgggaccat
     4981 cacgaacatg atcaccgcgg tcatgatcga gaacaggatc agcgagatgt actgcaggaa
     5041 cgcggtcagg ttgccgatcg gcatgtcgcc gctgttcacc cggtaggcgc ccagccacag
     5101 gaccgcgacg gtgctcaggt tcatgatcgc catcagcgac gggagcgtga tcgcgaacag
     5161 ccggttgacc cgcaggcccg tgtccatcag gtcggcgctc gcctcatcga agcgggcctc
     5221 ctcgtcgcgg gtccgcacga aggcgcggat cacgcgcacg cccgagagcg tctcgcgcat
     5281 cacgaggttg atccggtcga ccttcttctg catcacctgg aacaacggca cggccttgta
     5341 catgacgatg ccgatgaggg cgaacatgat cgggatgatc acgagcagga cgcccgacag
     5401 ggggatgtcc tggcggaggg ccatgatcag gccgccaacg accatgatcg gcgccgagat
     5461 catcacggtc agcgcgagca gcaggaccgt ctgcacctgc tgcacgtcgt tggtgttgcg
     5521 ggtgatcagg ctggccgcgc cgaacgtgtt gacctcgacc tgcgagaacg tctcgacctt
     5581 gcggaacacc tggctgcgga cgtcgcgccc gaagcccatc gcgaccttgg cgctccagta
     5641 cgcggcgacg accgcggcga cgccgaggat gaacgtcacc agcagcatga agccgccggt
     5701 gcgcaggatg tagtcgatgt caccggtgac gacgccgttg ttgatgatgt cggcgttgag
     5761 ctcggggaga tacaggttgg cgaccgcctg gatcgccagc aggatcatca cgaacacgag
     5821 cggccaccga tagggcggca ggtagcggcg gaacaagtcg atcaccggga tgcctcgtcg
     5881 gtcggcccgg aagtgggggt agcccacttg ggcgccagcc tcatccgggc ctcgcgcagc
     5941 gcgcgcgacc cacggaggaa gccgtcgagc tcctcgtccg acagctcccc gaggaggatg
     6001 ccgaggcgct cgcgccgtcc ggccgcgacc ccggccagga ggcgggtgcc gtcctcggtc
     6061 agcgcgacgc ggaccacgcg ccggtcctcg tcgtcgcggc ggcgctcgac gaggccgcgc
     6121 tgctccatgc ggtcgacgat gccggtggtg ctcgcctggg acacgtcgag cgactccgcg
     6181 agcgtcccca tcgggaccgg cccgtcggtc tcgaggacgg tcaggacctg gaggtggacg
     6241 aggctcagcg gtcccgacgg ccagcgccgc atgtagcgga gcatgtcggc cgggctgtgc
     6301 gaggtgagct cgtcgaggag gtcgttgagg agctgcttcc ggttggtggt cgtgggcgtc
     6361 atcgggctct tcacgttagg tagatagtca atgcgacctg aaggataagt cgccaggatc
     6421 cggccgtcaa acggcggcgc gtggcgcccg gccccggcgc gtggccttgt attcctcccg
     6481 ggaatgcgga tcgacgaccc ggccaccgtc ggctggccca caggtccaca gccgtgcggg
     6541 agcgctccta ctgacccggc cagatagtca tgcggtgagt gttgggccag gtcgaagtcg
     6601 cggcagttgg cctaacagtc aggtcgcgaa tacatggcca cccgacccgc ttggattggc
     6661 cgttcgcatt cactcggtgc atacgcggcc gactccgctc gggctcgcat tcacccggcg
     6721 aatacaaggc catgacccgc cggggagccg cgaggccgcg atcagggaac gcgcgccagg
     6781 ggattacccg gtgatcgcct tccggaagcg ccaggcggcc aggcccagga ggacgacggc
     6841 gaacccggtc agcaccgcca ggttcgtcgc caccgagtcg atgccgccgc cgtcggtgat
     6901 cagggactgg agcccggtca ccgcccagga gtgggggatc agcctcgcga gcgactgcat
     6961 cgtcgccggc atgatctgga gcgggatcat gcagccgccg agggccccca gcgcgagccc
     7021 gatgaacacg cccagcgacg acgcctggtc ggcgttgcgc gccatcgccc cgagcagcat
     7081 cgccgcgccg gccgagacca ggctgaacac caggatgatc gcggccgccc cgacggggtt
     7141 gccccaggac acgccgaaga acagcgcgga cacgaccacg atgtaggtcg cctggaacac
     7201 ggcgatcccg taccgcccca tcgcctcgcc ggcgatgatc gtcgccggtg acgtgggcgt
     7261 cgcgctcatg cg
//
LOCUS       DBNHXO010000131         2989 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1617845, whole genome shotgun sequence.
ACCESSION   DBNHXO010000131 DBNHXO010000000
VERSION     DBNHXO010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1617845"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="AC162A_02740"
     CDS             <1..665
                     /locus_tag="AC162A_02740"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR5009782.1"
                     /translation="RNVNIAFVNQLALLCERMGLDVWEVIDAAATKPFGFMRFTPGPG
                     VGGHCIPLDPYYLSWRAREFDFTDRFIELAGDINLAMPRHVVELVAEALNDRGRSLRD
                     ARVGILGVSFKPNVRDARNSPAADVIALVAERGAEVAFHDPHVPSFEDAGGVVRESRD
                     LGDVIEWADAIVVLVAHRAIDWDRVYREAALVVDTVNSSRERSTSERQVLRLGAGWSS
                     VG"
     gene            748..>2989
                     /locus_tag="AC162A_02745"
     CDS             748..>2989
                     /locus_tag="AC162A_02745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR5009783.1"
                     /translation="MTPRQDAAGSGEAGGQSPDSTTAPGADSLRTERIRILSDELRAT
                     RAELTASTQELDRTHRELAALRRRRSVRLALGVSRLIAPVAGAVRRVAPATARQGARQ
                     AVGAGEPRKRLRATEGEEARLRSRLASVLRPARSTSGPLVSIVIPTRDGLDHLRRLLP
                     ALDQIVYRDVEIVVVDNGSRDGTAAWLAANRPSFPTRVIANPENRSYADANNQGVAAA
                     TGELILLLNNDTEPAGPHVLGHMVDRVVDDATVVAVGSRLIYPRRSGPAMGPVSVAAD
                     LSLQHRGVAFATVDGTQVAVHVGRGEDPLGHDASERREIEMATAACLLVRRAAFEAVG
                     GFSGGYDYGMEDVDLALKLRAAGGRVIYEPQATFWHHESATQAAEARASRAERQTANR
                     ALLQERWGPRIARETFLDRLSAARSWASGPLHVGITLTRDDEHAGWGDWYTAHELGEA
                     LEAIGWRVSYLERWKEHWYTPDRSIDVVISLLDAFDVRRLPPGVVSVAWVRNWTERWL
                     GQPWFEDFDIVLASSQRSKDLIDGASVHVAGLMPLATNPVRFRGGDTREPVEGVDVAF
                     TANRWGEARGVEAIAPVLSAAGRSVAVFGKGWEAVPEMAGVTRGALAYADLPAAYADA
                     AIVIDDTATPTLPYGAVNSRVFDALAVGTLVITDNVMGARELFGASLPAAAEPDEIVR
                     LAERYLDDPEERRRLAIELRNIVIERHTYAHRAAELRDVLERWASAPRVDIAIGPPES
                     DLAQLWG"
BASE COUNT          413 a         1066 c         1075 g          435 t
ORIGIN      
        1 tccgcaacgt caacatcgca ttcgtgaacc agctcgccct gctatgcgag cggatgggcc
       61 tggacgtctg ggaggtcatc gacgccgcgg ccaccaagcc gttcgggttc atgcgcttca
      121 caccgggtcc cggcgttggt ggccactgca tcccgctcga tccgtactac ctctcgtggc
      181 gcgcccgcga gttcgacttc accgaccggt tcatcgagct cgccggcgac atcaacctcg
      241 ccatgccgcg ccacgtggtg gagctggtcg ccgaggcgct caacgatcgt ggccgctcgc
      301 tgcgggacgc ccgcgtcgga atcctcgggg tctccttcaa gcccaacgtc cgtgacgcgc
      361 gcaactcccc ggcggcggat gtcatcgccc tggtcgcgga gcgcggcgcg gaggtcgcgt
      421 tccacgaccc gcacgttccg tcgttcgagg acgccggcgg ggtcgtgcgc gagagccggg
      481 acctcggcga cgtcatcgag tgggccgacg cgatcgtcgt cctcgtcgcc caccgggcaa
      541 tcgactggga ccgcgtctac cgtgaggctg cgctggtcgt cgacaccgtg aacagctcgc
      601 gcgagcggtc gacgtccgag cgccaggtcc tgcgccttgg cgctggctgg tcgagcgtcg
      661 gctgaggggt cgcgcgccga tcgtgtccgc ccgcagggtt gaggggcccg gccgcccgct
      721 cgatccccct cgactatgct ggcccggatg actcctcgtc aggacgctgc cggatccggc
      781 gaggccggcg gccagtcgcc cgattcgacg accgcgccgg gcgccgactc gctgcgcacg
      841 gaacgcatcc ggatcctgtc cgacgagctc cgggcgaccc gggccgagct caccgccagc
      901 acgcaggagc tggatcggac ccatcgagag ctcgcggcgc ttcgtcggcg acggagcgtg
      961 cggctcgcgc tcggcgtgag caggctgatc gcacccgtcg ctggagcggt ccggcgggtc
     1021 gcgcctgcta ccgcgaggca gggtgccagg caggccgtgg gtgccggcga gccccgcaag
     1081 cgtctgcggg cgaccgaagg agaggaggca cgcctgcgct cgcggctggc cagcgtgctc
     1141 cggcccgcga gatccaccag cggccccctc gtatcgatcg tgatccccac ccgcgacggg
     1201 ctcgatcacc tgcggcgact gctcccggcg ctggaccaga tcgtctaccg cgatgtcgag
     1261 atcgtggtcg tcgacaacgg ctcgcgcgac gggaccgccg cctggctggc cgccaaccgg
     1321 ccctcgtttc ccactcgcgt catcgccaat cccgagaacc gctcgtatgc ggatgcgaac
     1381 aaccagggcg tggccgccgc gacgggcgag ctcatcctcc tgctgaacaa cgacaccgag
     1441 cccgcgggac cgcacgtgct cggccacatg gtcgatcggg tcgtcgacga cgcgaccgtg
     1501 gtcgcggtcg gcagccgcct gatctatccg cgccgcagtg gaccggccat gggcccggtg
     1561 tcagtagcgg ccgatctgag cctccagcac cgtggcgtcg cgtttgcgac ggtcgatggc
     1621 acgcaggtgg cggtccacgt gggtcgcggt gaggatccgc tgggccacga tgcgtccgag
     1681 cgacgtgaga tcgagatggc gaccgcggcg tgcctgctcg tccgtcgcgc cgcattcgag
     1741 gccgtcggcg gcttctcggg cgggtacgac tacggcatgg aggacgtcga cctcgcgctc
     1801 aagctgcggg cggcgggtgg ccgcgtcatc tacgagccgc aggcgacgtt ctggcaccac
     1861 gagtcggcga cgcaggcggc cgaggccagg gcgtcacgcg cagaacgcca aacggccaac
     1921 cgcgcgctgc tccaggagcg atggggaccg cgcatcgcac gcgagacctt cctcgacagg
     1981 ctgtcggcgg cgaggagttg ggcgtcgggc ccgctgcatg tcgggatcac cctgacccgt
     2041 gacgacgagc acgccggctg gggcgactgg tacaccgccc acgagcttgg cgaggcgctc
     2101 gaggcgatcg gctggcgggt gagctatctc gagcgctgga aggagcactg gtacacgccc
     2161 gaccgctcga tcgacgtcgt catctcgctc ctcgacgcat tcgacgtccg gcggcttcct
     2221 ccgggcgtcg tcagcgtggc gtgggttcgc aactggaccg agcgctggct cggacagccg
     2281 tggttcgagg acttcgacat cgtgctcgcg tcgagccagc gctcaaagga cctgatcgac
     2341 ggcgcgagcg tgcacgtcgc cgggctgatg ccgctggcga cgaatccggt ccggttccgc
     2401 ggcggcgaca cccgcgagcc cgtcgagggc gtcgacgtcg cattcaccgc gaaccgctgg
     2461 ggggaggcgc gcggggtcga ggcgatcgcc ccggtgctct cggcggcagg gcgctcggtc
     2521 gcggtcttcg gcaagggctg ggaggcggtc cccgagatgg ccggcgtcac ccgcggcgcg
     2581 ctcgcctacg ccgacctgcc agcggcctac gccgacgccg cgatcgtcat cgacgacacc
     2641 gcaaccccga cgctgccgta cggcgcggtc aactcgcgcg tgttcgatgc gctcgccgtc
     2701 gggacgctcg tcatcaccga caacgtgatg ggggcgcgag agctgttcgg tgcttccctg
     2761 ccggcggcgg ctgagcccga cgagatcgtg cggctagccg agcggtacct cgacgatccc
     2821 gaggaacggc ggcgcctcgc gatcgagctc cgcaacatcg tcatcgagcg gcacacctac
     2881 gcccatcgcg cggcggagct gcgcgacgtc ctggagcgct gggcgtcggc gccgcgggtc
     2941 gacatcgcga tcggtccgcc cgagagcgac ctcgcgcagc tgtggggtg
//
LOCUS       DBNHXO010000132         3458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3205297, whole genome shotgun sequence.
ACCESSION   DBNHXO010000132 DBNHXO010000000
VERSION     DBNHXO010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3205297"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="AC162A_02750"
     CDS             <1..306
                     /locus_tag="AC162A_02750"
                     /inference="COORDINATES: protein motif:HMM:NF021608.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HIR5009784.1"
                     /translation="MDAVGKDLVPGVERISARFHGRREQRTTFERAMLRITGMDLKME
                     QYRKGEAFVAGIEQRAGAVALRRLWDGPETLPTSAEIDDPAAWVRRMGLNQLGGGGS"
     gene            354..1367
                     /locus_tag="AC162A_02755"
     CDS             354..1367
                     /locus_tag="AC162A_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HIR5009785.1"
                     /translation="MPAAIALTPILAARYRARDIEAIRAASPGARIVTIGFDGHPDGA
                     LDDVEVMLRGRLPADTFDRILARAPSLRWVHSATAGVERVLTPASRDRGLTITNARGV
                     FSRPIAEYVMLMILAVSRRLPQLLELQAERTWQPLESRELRDVTVGIVGLGSIGRAVG
                     ALATAFGCRVIATRRRPEAGTEVADGSGDDPVLGSLMLDRVLPPDRLPELLAESDFVV
                     LAAPLTDDTRGLIGESAIVQLKPGAWVINVARGELVDERALGRALREGRLGGAVLDTF
                     REEPLPATSPLYDLPNVILTPHTSWSSTRVLERSVGLFCDNLGRYAAGKPLVNVVDPS
                     AGY"
     gene            1425..2531
                     /locus_tag="AC162A_02760"
     CDS             1425..2531
                     /locus_tag="AC162A_02760"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR5009786.1"
                     /translation="MQIAIVGLASAGKTTVFNTLTRGHAETGGYGALQLNVGVVKVPD
                     ARLERLADLFHPKKVVHADVTYVDLPAPPASSEGRVGTDELPAEHMARLREADALIHV
                     VRAWDDAAHPHPAGSVDPARDIEQLDLEFMLADLAMIERRLERLNAGGGRHGTPAERE
                     ANEREGQILVRLKAVLEGDRPIRDEALDADDEKAIRGFRFLTQKPVLILVNVGEGDLA
                     GAGAVVERISGAYGHSHAMVDSLSAKIEMELGELEPDEAGVFMEELGLAESSLDRVIA
                     LSYRLVGLISFLTAGPDEVRAWPVPDGSNAVDAAGAIHTDLAKGFIRAETIGYEDLLA
                     LGSIAEAKKHGKVRSEGKTYRVRDGDVIEILFSK"
     gene            complement(2535..2879)
                     /locus_tag="AC162A_02765"
     CDS             complement(2535..2879)
                     /locus_tag="AC162A_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009787.1"
                     /translation="MHFYELHEGDNDTFVDLLLVREDEMEPEEFFEVVQSIRRRIQDS
                     YERETLIESIAEELERDYDFIAVSDERLTAAINVSKEEDDNFLADLEAPEPDVDYVSI
                     LADLQAGDSRLN"
     gene            2982..>3458
                     /locus_tag="AC162A_02770"
     CDS             2982..>3458
                     /locus_tag="AC162A_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR5009788.1"
                     /translation="MADAPAVVVVGAASRDLVHDDTRGWRLGGGVSYAALTLARLGLR
                     VGALVGADRLAAESRELHLLRDAGVEVVVAPLARGPVFINTETPTGRIQHAPQVSDPI
                     EPSALPPGWRDAAGWLFGPVAAELPDGWADVPARDAQVALGWQGLLRVLVTGEPVTQ"
BASE COUNT          461 a         1219 c         1287 g          491 t
ORIGIN      
        1 atggacgcgg tcggcaagga cctcgtgccc ggggtcgagc ggatctccgc gcggttccat
       61 ggccgccggg agcagcgcac gacgttcgag cgcgccatgc tgcggatcac cggcatggac
      121 ctcaagatgg agcagtaccg taagggcgag gcgttcgtcg cgggcatcga gcagcgggcg
      181 ggcgcggtgg cgctgcggcg cctgtgggat ggacccgaga cgcttccgac ctcggccgag
      241 atcgacgatc ccgccgcctg ggtgcggcgg atggggctga accagcttgg aggaggcggc
      301 tcgtgaggca gcccggggcc agcgccccag gaccaggatc ggggccaggt ggggtgcccg
      361 cggcgatcgc gctcaccccg atcctcgccg cgcgctatcg cgcccgggac atcgaggcga
      421 tccgggcggc ctcgccgggc gcccggatcg tgaccatcgg cttcgacggc cacccagacg
      481 gggcgctgga cgacgtcgag gtcatgctcc gcggacgcct gcccgccgac acgttcgacc
      541 ggatcctcgc gcgggcgccg tccctgcgct gggtacactc ggcgaccgcg ggcgtcgagc
      601 gcgtcctcac gcccgcgagc cgtgaccgcg ggctgacgat cacgaacgcg cgaggcgtgt
      661 tctcgcgccc gatcgccgag tacgtgatgc tgatgatcct cgccgtctcg cggcggctcc
      721 cgcagctgct cgagctccag gccgagcgca cgtggcagcc gctcgagtcg cgcgagctgc
      781 gcgacgtcac cgtgggcatc gtgggcctgg ggtcgatcgg gcgagcggtc ggcgccctcg
      841 cgacggcgtt cggctgccgg gtcatcgcca cccgacgccg gccggaggcg ggcaccgagg
      901 tggccgacgg gtcgggggac gacccggtgc tcgggtcgct gatgctcgac cgtgtcctgc
      961 cgcccgaccg gctgcccgag ctgctcgccg agtccgactt cgtcgtgctc gcggctccgc
     1021 tgaccgacga cacgcgaggg ctgatcggcg agtccgcgat cgtgcagctc aagccgggtg
     1081 cgtgggtgat caacgtcgcg cgtggcgagc tcgtcgacga gcgagcgctg ggccgcgcgc
     1141 tccgcgaggg ccggctcggc ggcgccgtgc tggacacgtt ccgcgaggag ccactgcccg
     1201 cgacctcgcc actgtacgac ctgcccaacg tcatcctcac gccgcacacg tcgtggtcgt
     1261 ccacacgggt gctcgagcgg tctgtcggcc tgttctgcga caacctcggg cgctatgccg
     1321 ccggcaagcc gctggtcaac gtcgtcgacc ccagcgcggg gtactgacgg gcgccggttg
     1381 gccgcgacgc cgggaacggc cctcccgacc ggtaggatcg ccccatgcag atcgccatcg
     1441 tcggcctggc ctcggccggc aagaccaccg tgttcaacac gctcacgcgc ggccacgccg
     1501 agaccggcgg ctacggcgcc ctccagctga acgtcggcgt cgtcaaggtc cccgacgccc
     1561 ggctcgagcg cctcgccgac ctgttccatc cgaagaaggt cgtccacgcc gacgtgacct
     1621 acgtcgacct gcccgcgccg ccggcctcgt cggagggtcg ggtcgggacc gacgagctgc
     1681 cagccgagca catggcgcgg ctgcgcgagg cggacgcgct gatccacgtc gtgcgggcct
     1741 gggacgacgc cgcgcatccg catccggcgg gctcggtcga cccggcgcgc gacatcgagc
     1801 agctggacct cgagttcatg cttgccgacc tcgcgatgat cgagcggcgg ctggagcgcc
     1861 tgaacgccgg tggcgggcgc cacgggaccc ccgccgagcg cgaggccaac gagcgcgagg
     1921 ggcagatcct cgtgcgcctc aaggccgtgc tggagggcga tcggcccatc cgcgacgagg
     1981 cgctcgacgc ggacgacgag aaggccatcc gcgggttccg attcctgacc cagaagcccg
     2041 tgctcatcct cgtcaacgtc ggcgaagggg acctcgcggg cgccggggcc gtcgtcgagc
     2101 ggatctcggg cgcctacggg cacagccacg cgatggtcga ctcgctatcg gcgaagatcg
     2161 agatggagct gggcgagctc gaacccgacg aggcgggcgt gttcatggag gagctgggcc
     2221 tggccgagtc gagcctcgac cgggtcatcg ccctgtccta ccggctggtc ggcctgatct
     2281 cgttcctcac cgccggcccc gacgaggtgc gtgcgtggcc ggtccccgat ggctccaacg
     2341 cggtcgacgc cgcgggcgcg atccacaccg acctcgcgaa gggcttcatc cgggccgaga
     2401 cgatcggcta cgaggacctg ctggcgctgg gctcgatcgc cgaggcgaag aagcacggca
     2461 aggtccgctc ggagggcaag acctatcgcg tccgcgatgg cgacgtgatc gagatcctgt
     2521 tcagcaagta gccgtcagtt gaggcggctg tcgcccgcct ggagatcggc caggatcgac
     2581 acgtagtcga cgtcgggctc gggcgcctcg aggtccgcca ggaagttgtc gtcctcctcc
     2641 ttcgagacgt tgatcgcggc ggtcagccgc tcgtccgaaa ccgcgatgaa gtcgtagtcg
     2701 cgctcgagct cctcggcgat cgactcgatc agcgtctcgc gctcgtacga gtcctggatc
     2761 cgccggcgga tcgactggac gacctcgaaa aactcctccg gttccatctc gtcctcgcgg
     2821 acgaggagca ggtcgacgaa ggtgtcgttg tcgccctcgt ggagctcgta gaagtgcatg
     2881 attggaacct cggtcggggg cgggttggcc cgccggtgcg cgggatgggc gcggtccgac
     2941 cagtataggc gggccccagc cgggccccga cgggaggcga ggtggcggac gcgccggcgg
     3001 tggtcgtggt cggtgcggcg tcgcgtgacc tcgtccacga cgacacccgc ggctggcggc
     3061 tgggcggcgg ggtctcgtac gccgccctga cgctcgcgcg tctcggactg cgcgtcgggg
     3121 cgctcgtggg cgcagaccgg ttggcggcgg agtcgcgcga gttgcacctc ctgcgcgatg
     3181 cgggggtcga ggtcgtcgtc gcgccgctgg cgcgcggtcc cgtgttcatc aacaccgaga
     3241 cgccgacggg ccggatccag cacgcgcccc aggtctccga cccgatcgag ccctcggcgt
     3301 tgccgccggg ctggcgcgat gccgcgggct ggctgttcgg gcctgtggcc gccgagttgc
     3361 ccgacggctg ggccgacgtg cctgcgcgag acgcgcaggt cgccctcggc tggcaggggc
     3421 tcctccgggt gctcgtcacc ggcgagcccg tgacccag
//
LOCUS       DBNHXO010000133         1697 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4792959, whole genome shotgun sequence.
ACCESSION   DBNHXO010000133 DBNHXO010000000
VERSION     DBNHXO010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4792959"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /gene="argG"
                     /locus_tag="AC162A_02775"
     CDS             complement(<1..677)
                     /gene="argG"
                     /locus_tag="AC162A_02775"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR5009789.1"
                     /translation="MNKVVLAYSGGLDTSVAVAWLREQYGVDVITLTVDVGGGSLREG
                     VERRAMSAGAAKAYVVDARETFISQYVWRSLQANALYQGVYPLATALARPLIAQLLVE
                     VAQREGADAVAHGCTGKGNDQVRFDVAVHALDPGLEVVAPMRVGMGLSRDQEIDYAAE
                     RGIEIPITKASPYSIDVNLWGRSCETGVLEDPWVTPPPDAYEWTVAAGDAPAPIELVI
                     GFEGGIPV"
     gene            complement(674..1180)
                     /locus_tag="AC162A_02780"
     CDS             complement(674..1180)
                     /locus_tag="AC162A_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0034618 - arginine binding [Evidence IEA];
                     GO_process: GO:1900079 - regulation of arginine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIR5009790.1"
                     /translation="MTMIVVKRDRHAAIRRLVGARAIGSQGELVSELSALGFDVTQAT
                     VSRDIAELGLAKVMRGDHSVYVNPEALGPATRPASDERLRRILADIPVSIGRSGLIVV
                     LTGQPGTANVIAQAIDESTLTDQVGTVAGDNTLIVLFPDEPALDRWLERFETLQPPEE
                     AALEATSR"
     gene            complement(1177..>1697)
                     /locus_tag="AC162A_02785"
     CDS             complement(1177..>1697)
                     /locus_tag="AC162A_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR5009791.1"
                     /translation="DVGCDAVGLSGVDGGLLIGQRLPNVGLVATVVGIRRDLIDSLLV
                     ADQVPVVAPLARDEDGVVCNVNADDAAAGLAAGLGARQLVLMTDVDGVRNADGTKLDS
                     ITADEAEVMIADGTIAGGMVPKVRAALAALAWDDAEAIIADSSAPDALTRALDDPTFG
                     TRVSARARAGAA"
BASE COUNT          234 a          607 c          623 g          233 t
ORIGIN      
        1 accgggatcc cgccctcgaa gccgatcacg agctcgatcg gggccggcgc gtcgcccgcg
       61 gcgacggtcc actcataggc gtcgggcggc ggcgtgaccc atgggtcctc gagcacaccg
      121 gtctcgcacg agcggcccca gaggttgacg tcgatcgagt acggggacgc cttcgtgatc
      181 gggatctcga tgccccgctc ggcggcgtag tcaatctcct ggtcgcgcga caggcccatc
      241 ccgacgcgca tcggggccac gacctcgagc ccggggtcga gcgcgtgcac cgcgacgtcg
      301 aaccgcacct ggtcgttgcc cttgccggtg cagccgtggg caacggcgtc ggcgccctct
      361 cgctgggcca cctcgaccag cagctgggcg atcagcggcc gggcgagtgc ggtcgcgagc
      421 ggatagacgc cctggtacag cgcgttcgcc tgcagcgagc gccagacgta ctgcgagatg
      481 aacgtctcgc gcgcgtcgac gacgtacgcc ttggcggcgc cggccgacat ggcgcggcgc
      541 tccacgccct cgcgcagcga gcccccgccg acgtcgacgg tgagcgtgat gacgtcgacg
      601 ccgtactgct cgcgcagcca ggcgacggcg acggaggtgt cgaggccgcc ggagtacgcg
      661 agcacgacct tgttcatcga gaggtggcct ccagtgccgc ctcctcgggc ggctgcagcg
      721 tttcgaatcg ctcgagccag cgatccagcg ccggctcgtc ggggaacagc acgatcagcg
      781 tattgtcgcc cgccacggtg ccgacctggt cggtcagcgt ggactcgtcg atcgcctggg
      841 cgatcacatt ggcggtgccg ggttgcccgg tgaggacgac gatcagcccg ctgcggccga
      901 tgctcaccgg gatgtcggcg aggatgcgcc gcagccgctc gtcggacgcc ggccgggtgg
      961 ccggcccgag cgcctcgggg ttgacgtaga ccgagtggtc gccgcgcatc accttggcga
     1021 ggccgagctc cgcgatgtcg cggctcacgg tggcctgggt gacgtcgaag ccgagcgcgg
     1081 acagctccga gaccagctcg ccctggctgc cgatggcgcg cgcgccgacg aggcggcgga
     1141 tcgcggcgtg gcgatcgcgc ttgaccacga tcatcgtcat gccgccccgg cgcgtgcgcg
     1201 cgccgagacg cgggtgccga acgtcgggtc gtcgagggcg cgggtaagcg cgtcgggcgc
     1261 cgaggagtcg gcgatgatcg cctcggcgtc gtcccacgcc agcgccgcca gggccgcacg
     1321 gaccttgggc accatgccgc ccgcgatcgt gccgtcggcg atcatgacct cggcttcgtc
     1381 ggccgtaatc gagtccagct tggtcccatc agcattccgg actccgtcca cgtcggtcat
     1441 cagcacgagc tggcgggcgc cgaggcccgc ggcgagtccg gcggccgcgt cgtcggcgtt
     1501 gacgttgcac acgacgccgt cctcgtcccg cgcgagcggc gcgacgacgg ggacctggtc
     1561 ggcaacgagc agcgagtcga tgaggtcgcg gcggatcccg acgacggtcg cgaccagccc
     1621 gacgttgggt aggcgctggc cgatgagcag cccgccatcc acgcccgaga gcccgaccgc
     1681 gtcgcagccg acgtccc
//
LOCUS       DBNHXO010000134         2067 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3006933, whole genome shotgun sequence.
ACCESSION   DBNHXO010000134 DBNHXO010000000
VERSION     DBNHXO010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3006933"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="AC162A_02790"
     CDS             complement(<1..349)
                     /locus_tag="AC162A_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR5009792.1"
                     /translation="MIGGRRPISGRKPGDKRVRVERHHAPYFRYTGPGQMTAKAAASA
                     PTTTMGRGVARLKHTLLGRPLANEEEIGQRLSKTKALAIFSSDAISSSAYATEEIILA
                     FILAGVAGAAFNLS"
     gene            complement(362..1084)
                     /locus_tag="AC162A_02795"
     CDS             complement(362..1084)
                     /locus_tag="AC162A_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR5009793.1"
                     /translation="MRVAVLSDLHANIAALEAVLAVMPSVDEVWQLGDVVGYGPQPDA
                     VVARLREIGARGVRGNHDAAAIDQLDIESFNVDARGAMEWTRATIADETRAWLAALPE
                     RLEREDYLLVHGSPRDPTWEYITTTPVARAAMAAMPTRSGLHGHTHVPIAFVEEDGRL
                     DTMSPGAGSHITFDGRRVMLNPGSVGQPRDGIPTTSWLLLDTGDGSATWQRTAYDVST
                     VQAEMLALGLPNRLIERLSYGL"
     gene            complement(1135..1932)
                     /gene="rsmG"
                     /locus_tag="AC162A_02800"
     CDS             complement(1135..1932)
                     /gene="rsmG"
                     /locus_tag="AC162A_02800"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR5009794.1"
                     /translation="MHDFEEGALDRPRGPLPTRVSDVPELPPGYGDALEAGLASLDLR
                     LEPDVLAAIDGHVRLLLAWTQAINLTGIREPAAVALGHVVDSLTALPLLRARGITRLL
                     DLGSGGGFPGLPLAAALPAERALLVDSIAKKVGFLRAVIDATGLADRVAAEAVRAEAL
                     AHDPRDREAWPAVVTRAVAPLAELVEVGLPLVAPGGVLVAWKREPLDAEIAAAAPALA
                     TLRAGRLEVVDPGVAGLERHRLVVVERGGPIDATFPRDAAERRRRAS"
BASE COUNT          265 a          743 c          783 g          276 t
ORIGIN      
        1 ccgacaggtt gaacgccgcc ccggcgaccc cggcgaggat gaacgcgagg atgatctcct
       61 cggtcgcgta ggccgacgac gagatcgcat ccgagctgaa gatcgcgagc gccttggtct
      121 tcgacaggcg ctggccgatc tcctcctcgt tggcgagcgg gcggccgagg agggtgtgct
      181 tgagacgggc gacaccccgg cccatcgtcg tcgtcggcgc gctggcggcc gccttcgcgg
      241 tcatctggcc cgggcccgtg taccggaagt acggcgcgtg gtggcgctcg acccggaccc
      301 gcttgtcgcc gggcttgcgc ccgctgatcg gccgccggcc gccgatcatc ggcggctcgt
      361 cctacagccc ataggacagc cgctcgatga gccggttcgg gagcccgagc gccagcatct
      421 cggcctggac ggtcgagacg tcgtaggcgg ttcgctgcca ggtcgccgag ccgtcgccgg
      481 tgtcgagcag gagccacgag gtggtcggga tcccgtcacg tggctggccc acgctgccgg
      541 ggttgagcat gacccggcgg ccgtcgaagg tgatgtgcga gccggcgccc ggactcatgg
      601 tgtccaatcg cccgtcctcc tcgacgaacg cgatcgggac gtgggtgtgt ccgtggaggc
      661 cgctccgggt gggcatcgcc gccatcgcgg cgcgggccac cggggtcgtc gtgatgtact
      721 cccaggtcgg gtcgcggggg ctgccgtgga cgagcaggta gtcctcgcgc tcgagccgct
      781 cggggagcgc cgcgagccac gcgcgtgtct cgtcggcgat cgtcgcgcgg gtccactcca
      841 tcgccccccg cgcgtcgacg ttgaacgact cgatgtccag ttggtcgatc gccgccgcgt
      901 cgtgattgcc tcgaacgcct cgggcaccga tctcgcgcaa ccgggcgacg accgcgtccg
      961 gctgcggccc gtagcccacc acgtcgccca gctgccagac ctcgtcgacc gacggcatca
     1021 ccgccagcac cgcctcgagg gccgcgatgt tggcatggag gtccgacagg acggcgacgc
     1081 gcaccggggg agggtagcgc agcgggatcg gcggagagcg tgggtgcccg ccactcacga
     1141 cgcccggcgt cggcgctccg ccgcgtctct cgggaaggtc gcgtcgatgg gaccgccgcg
     1201 ctcgacgacg accagccggt gccgctccag cccggcgacc ccggggtcca cgacttcgag
     1261 gcgacccgcg cgcagcgtgg ccagggccgg agccgctgcg gcgatctcgg cgtcgagcgg
     1321 ctcgcgcttc cacgcgacga ggacgccgcc gggagccaca agcgggaggc ccacctcgac
     1381 gagctcggcc agcggcgcga cggcgcgggt gacgaccgcg ggccaggcct cgcggtcgcg
     1441 cgggtcgtgc gcgagcgcct ccgcgcgaac cgcctctgcg gccacgcggt ccgcaaggcc
     1501 ggtcgcgtcg atcacggctc gcaggaagcc gaccttcttg gcgatcgaat cgacgagcag
     1561 cgcgcgctcg gcgggcaggg ccgcggcgag gggcaggccc gggaatccgc cgccgctgcc
     1621 gaggtcgagc aggcgggtaa tcccgcgcgc gcggaggagc gggagggcgg tcaggctgtc
     1681 gacgacgtgc ccgagggcga cggccgcggg ctcgcggatc cccgtgaggt tgatcgcctg
     1741 cgtccaggcc aggagcagtc gcacgtggcc gtcgatggcg gcgagcacgt ccggctcgag
     1801 ccgcaggtca agggacgcga ggccggcctc gagggcgtcg ccgtaccccg gcgggagctc
     1861 gggaacgtcc gaaacgcgcg tcgggagcgg acctctcggc cggtccaggg caccctcctc
     1921 aaagtcgtgc accgggccgt ccgggaaccg ctcccggcgt cgttcgttcg cggcaggcgt
     1981 cctgttcggt ctggcgtcgt ccggtcggtt ccggcccggg tttccccgtg acccctcccg
     2041 gacgacgttc cccgaaaact tgcgcgc
//
LOCUS       DBNHXO010000135         7504 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4397045, whole genome shotgun sequence.
ACCESSION   DBNHXO010000135 DBNHXO010000000
VERSION     DBNHXO010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 7504)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7504)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7504
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4397045"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="AC162A_02805"
     CDS             complement(<1..1086)
                     /locus_tag="AC162A_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009795.1"
                     /translation="MVAGSLAVLLVSQSAFAAVTWSKPVNAGPQYSWNLGRGLATTDT
                     SSKTYLHVQFEDDGHNHTGIYYRRGNASGSSWGSAKRLNPASSDAEAGAIAAAGRFVY
                     VAWVTGTHAANDYDPAAPRHIRVRVNTNHGASNAWQTAKKFEAPERTGRPSIAAWGSR
                     VYVAYTDADTGNIYVATNNGVNSEDIGWLAGQAGVTTRTSTIDGDGFEGTPVVAASGA
                     NVLVAWLDSDDGTIKARISTDHGHTWPDPAIDLATGQAYGMSAAADATRLALAWAAPD
                     GIRSRTFRDDTWKATHKLASFSSTGAYRNGYGTSIALAGSSRLGVAWSACTRANCAGS
                     STQGVNVRWRESANNGSSWKPSVTIASH"
     gene            2004..3680
                     /locus_tag="AC162A_02810"
     CDS             2004..3680
                     /locus_tag="AC162A_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009796.1"
                     /translation="MGEAVHDTVAQTLYLAIRFENLSNIYAGLVTEAAVLLDGQELSL
                     FPLVPPSSIPPGLSSDITYEVSVGEGDPLKDGVLRWGRSDETQTTIRLADGAVEGFGV
                     PRDLAIDGWGQIGREAVHLTGAVLFADAIRAPRAPTGQHVLRLVFDEFASSPSVVNGF
                     VPSDQLTLAWPDGTTHDALSGSDGRLPQMSWTSTTGNYADFAVPADPAGQYQALLASV
                     SRSAMGTFHTDLIDRVPISFALDQATVTTSPPGSAIVNLPRAILPPAEGDPAPVAVDL
                     ALTTGEVNLPGIGYRAKHLRWDPATATASIDGEAWLMKPVVPPDDGLLSTPSIFQPWM
                     HLRIGDRLYNGTLHGTEIPTITPDAPVPVTYDFAFMPAMDPAGATLFLGQDYTAPSVL
                     PLVPDSGDAIDPPLPVEVPVSAPAVSAGNYTVQLESYRLGLPREGVLPPTGMRALEVI
                     FDVTAHQVENPGAFGLLFATGTQLFLTAPDGYLQQAASADTAYFDNDGETRRVAATFF
                     VPASWNGGTLPFTVRSRDEIRDITGDHWTEVSFGAEFEPLDAQPTEGISQ"
     gene            3701..5293
                     /locus_tag="AC162A_02815"
     CDS             3701..5293
                     /locus_tag="AC162A_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009797.1"
                     /translation="MTKPSRRPPARQGAAVLGLVGILVSALLGGCSPAPEPKLNLNGI
                     EYTQAEFKYGLNVNPDPSVKLADDVVLVPNGAQAVRSITGDGLTWTLDPKAPGVDGLQ
                     VGKVMFLTGRGVGRIVDVTSADAGSEVTIAPVDITEVITEGDFSGDDVALDESQMIVY
                     DTGELPWSVIPLGPSGQPADDASPAPPGTPSSDPPASPSSVAAARLARYAVPGRDVGD
                     EPVPLDEEEYQPGDVSQRDPPPDPSWGNAGPMDVGDFHLTPKCCKSGVGVSIEYDKDG
                     TRVKGTLEVVGTLPRASWHLRISGGTLESAGLTLTGSAGIHYVFSAATKTGLVGNLHR
                     YVQLPASLEWNQVIGGIPFAFGLTQSFIIRTAFSSKDNHLDAEGLWSFSGGLGFAYTS
                     ATGLQVTKPADVSTTRSMLTSMGGGGTGAEGIVLAHRLEARIGIGVTNFSVGPFVDLV
                     SSVGASRGSDIGILICKGVNLNIATGAGISYRLPEIVVKIVNFVLKLFQAKPILSQGN
                     IARVEIPVLNRSAVFPESKICR"
     gene            complement(5375..6430)
                     /locus_tag="AC162A_02820"
     CDS             complement(5375..6430)
                     /locus_tag="AC162A_02820"
                     /inference="COORDINATES: protein motif:HMM:NF020650.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5009798.1"
                     /translation="MRPFPATARATLIAALAFVVAACGTGASRSPGTPSAQPESVAPN
                     ESTEPSAAPVALTVGLGYIPNVQFAQFYLAQQSGAYADAGLDVTLQNKIDPDLITLLG
                     QGAVDIGSGDGTSVIPAVSQGIPVVYTATIYGKFPSIVLARADSGITKAADLRGKKIG
                     IPGKYGSSWIMLQALLQSGGLTPADVDIVEFPDFGQAAALQQGAVDAATGFANNEPIQ
                     LRKAGIEPVVLTVDDIVPLPGPGLVTGKATLETKRDALGAFTLVTLNAMDEIAADPQK
                     GLDATFAAVPDLAKDPELQRQILDATIATWQNPRTDAYGAIDRDGWQQSLEFMTTLGL
                     VPNPVTVDQLVDETLLP"
     gene            complement(6466..7281)
                     /locus_tag="AC162A_02825"
     CDS             complement(6466..7281)
                     /locus_tag="AC162A_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009799.1"
                     /translation="MSRAVPSSLATQGSAERPARRRRWLELAIPVVVSLALFVLVWQL
                     IVFLTGYPSFILPGPLTVAQRFVTAWTDGTMGRHAATTLVEILLGFAIGSGLALIAGL
                     ALARSRLVERLVSPYLVAAQSTPILALAPLLVLWFGTGLLSKLVITTLIVFFPVAIAT
                     MVGIRTVDPGLIEMARSFRATGRQIATRIEIPAALPSILGGMRVGATLAVIGAIVAEW
                     AGGESGLGVLINIARGSLFDIPLMFATLLTLALIGVALYLVMVLIERRLVGDR"
     gene            complement(7278..>7504)
                     /locus_tag="AC162A_02830"
     CDS             complement(7278..>7504)
                     /locus_tag="AC162A_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5009800.1"
                     /translation="WQRIGTTIVLVTHSISEAVFLADRVVVMSPRPGRIVADVPVGLQ
                     RPRRMADVDSARAAEIAAQVRSHLAMDGES"
BASE COUNT         1126 a         2602 c         2648 g         1128 t
ORIGIN      
        1 gtgcgatgcg atcgtcaccg acggcttcca gctgctgccg ttgttcgccg actcccgcca
       61 ccggacgttg acgccctggg tggaggagcc ggcgcaattc gcgcgcgtgc acgccgacca
      121 ggcgacaccc agccggctgg acccagccag ggcgatcgag gtgccatagc cgttgcggta
      181 ggcgcccgtc gagctgaacg aggcgagctt gtgcgtcgcc ttccacgtgt cgtcccggaa
      241 cgtccgcgac cggatgccgt ccggcgccgc ccaggcgagc gcgaggcgcg tcgcgtcggc
      301 ggccgccgac atgccgtacg cctggccggt cgcgaggtcg atggccggat cgggccaggt
      361 gtgaccgtgg tcggtcgaga tcctggcctt gatcgtcccg tcgtcgctgt ccagccaggc
      421 gacgaggacg ttggcgcccg aggccgcgac gaccggcgtg ccctcgaacc cgtcgccgtc
      481 gatggtgctc gttcgcgtgg tgacgccggc ctgcccggcc agccacccga tgtcctcgct
      541 gttcaccccg ttgttggtcg ccacgtagat gttgccggtg tccgcgtccg tgtaggccac
      601 gtagacgcgg ctgccccagg cggcgatcga cgggcggccg gtccgctccg gggcctcgaa
      661 cttcttcgcc gtctgccagg cgttcgatgc gccgtggttg gtgttcacgc gcacgcggat
      721 gtggcgggga gccgccggat cgtagtcgtt ggcggcgtgg gtcccggtca cccacgcgac
      781 gtagacgaac ctgccggccg ccgcgatcgc gccggcctcc gcgtccgagc tggcggggtt
      841 cagccgcttc gccgagcccc acgacgatcc cgaggcgttg ccgcgtcggt agtagatgcc
      901 ggtgtggttg tggccgtcgt cttcgaactg gacatggagg taggtcttcg aggaggtgtc
      961 ggtggtcgcg aggccgcggc cgaggttcca cgagtattgc gggccggcgt tgacgggctt
     1021 cgaccaggta accgccgcga atgcggactg gctcaccagc aggaccgcca gggatccggc
     1081 gaccacgggt agaagcgtgc ggcgcggccg gtgtcggcgc ggagagagca tcgggtcaac
     1141 ctccacggtg acggacatgg ggcctagacc gctgcgacgt ctcccattat tgtgcgaatc
     1201 ggacgcaagc ccaacgtcac gagggctggt tgcgaaggag gggcccctgg agtgaggcgg
     1261 gacgagtcgc ggctcggcgt gctgttcgtc gtcgccggtc tcgtgctgac cgcatgcggc
     1321 actccagcgc ccaccggccc ctcctgggtc gacagggccc acgtcgacgc cgtgccgccc
     1381 accgaagggt ccggcgagtt cccccagcca gggccgggca ccggcggctg cgcactggtt
     1441 gacgacgtca cccggcccga cgagagcgga ggcaccgggt cggctcccgg cggaccggtt
     1501 agcgccgggg tgggcaacga tccctacacg gcgacgggag cgcgccaggt gcccgggccg
     1561 aacatgcacg tgtcggatcc gccggcgtca ccggtcacca cggtggtcgc cgccccggtc
     1621 gaccccgatg agcacgtagc tgcacgacag cctgacggca acgtaacggg accggtgccg
     1681 ccaccaaagg tcatcgatgg aactcgacct atgccgtccg tcgatccgtc ggccgcgccg
     1741 gggtccgcca caggcgtacc ggggcccgga gtagcctgcc cgggcggggg cttgtgaccc
     1801 gccacgcgac gcgacctctc gccaccatcg cccttggact cctgacggcc atgctcgcag
     1861 gttgcgggac caccgtaacg cccacgccgt ctccgacccc cggccagact ccgctcccct
     1921 cacctagcgg gccgtcccgg cccgcatccc agcccagccg cgacatcggc gcgcggttcc
     1981 agtccggccc gttcgtgcta accgtgggcg aggcggtaca cgacacggtc gcccaaaccc
     2041 tgtacctcgc catccggttt gagaacctca gcaacatcta tgcgggcctg gtcaccgagg
     2101 cggccgtcct gctcgacggg caggagctct cgctgttccc gctcgtcccg ccgtcgtcca
     2161 ttccgcccgg cctgtcgagc gatatcacct acgaggtgag cgtgggcgag ggcgatccgc
     2221 tgaaggacgg ggtgctgcgc tggggacgca gcgacgagac acagaccacc atccgcctcg
     2281 ccgacggcgc cgtggagggc tttggcgtcc cgcgcgactt ggccatcgac ggctggggcc
     2341 agatcggacg cgaggcggtg catctcacag gagccgtgct gttcgccgac gccatccgcg
     2401 ctcctcgggc acccaccggc cagcacgtgc tgcgcctggt attcgatgag ttcgcgagtt
     2461 cgcctagcgt cgtcaacggc ttcgtcccat ccgaccagct cacgctcgcc tggcccgacg
     2521 ggacgaccca cgacgcgctg tccgggagcg acggacggct cccgcagatg agctggacgt
     2581 cgacaacggg caactacgcc gacttcgccg tgccggcgga tcccgcgggc cagtaccagg
     2641 cactgctcgc gagcgtgtcg cggtccgcga tgggcacgtt ccacacagac ctcatcgacc
     2701 gcgtcccgat ctccttcgcc ttggaccagg cgactgtcac aacgtcgcca ccgggatccg
     2761 ccatcgtcaa tctgcctcgc gcgatcctcc cgccggcaga gggcgacccg gcgccggtcg
     2821 ccgtcgacct ggcgctgacc acgggcgagg tcaacctgcc tgggatcggg tatcgggcca
     2881 aacaccttcg ctgggatccc gccaccgcga cggcgagcat cgacggcgag gcgtggctga
     2941 tgaagccggt ggtcccgcct gacgatggac tcctgtcaac gccatcgatc ttccagccct
     3001 ggatgcacct gcggatcggt gaccggctgt acaacgggac gctccacggg acggagatcc
     3061 ccaccatcac ccccgatgcg cccgtacccg tcacctacga cttcgcgttc atgccggcta
     3121 tggatccggc tggcgcaacc ctgtttctcg ggcaggacta cacggcgcct tcggttcttc
     3181 cgcttgtgcc cgacagcggg gacgccattg atccaccgct gccggtcgag gtgccggtca
     3241 gtgcaccggc cgtttctgcc ggcaactaca ccgtgcagct ggagagctat cggctcggtc
     3301 ttccgcgcga gggggtcctt ccgcccacgg gcatgcgcgc gctcgaggtg atcttcgacg
     3361 ttacggccca ccaggtcgag aaccccggcg ctttcggcct gctgttcgcg acggggaccc
     3421 agctgttcct gacggcgccc gacggctacc tgcaacaggc tgccagcgcg gacacggcgt
     3481 acttcgacaa cgacggcgag acgcggcggg tggcggcgac gttcttcgtc ccggcgagct
     3541 ggaacggtgg aaccttgccg ttcacggtcc gcagccgcga cgagataagg gacatcaccg
     3601 gcgaccactg gaccgaggtg tcgttcggcg ccgagttcga gccccttgac gcccagccga
     3661 cggaagggat cagccagtga cgaccgcgtt ccgatcgccg atgacgaagc ccagccgacg
     3721 gccaccggcg cgtcaaggcg cggccgtgct cgggctggtc ggcatcctcg tgtccgccct
     3781 gctcggcggc tgctcgcccg ccccggagcc caagctcaac ctcaacggca tcgagtacac
     3841 gcaggccgaa ttcaagtacg gcctcaacgt caacccggac ccgagcgtga agctcgccga
     3901 cgacgtagtc ctcgtcccga acggtgccca ggccgtgcgc tcgatcacgg gcgacgggct
     3961 gacctggacg ctggatccca aggcgcccgg cgtcgacggg ctccaggtgg gcaaggtcat
     4021 gttcctgacc ggccgcggcg tcggccggat cgtcgacgtc accagtgccg acgccgggag
     4081 cgaggtcacg atcgcgccag ttgacatcac cgaggtcatc acggagggcg acttctcggg
     4141 cgacgacgtg gccctcgacg agagccagat gatcgtctac gacaccggcg agctgccgtg
     4201 gtcggtgatc ccactcggcc ccagcgggca gcccgcggat gacgcgtcgc ctgctccacc
     4261 gggcacgccc tcgagcgatc ctccggcatc gccctcgtcc gtcgccgccg cacgcctggc
     4321 ccggtacgcg gtgccgggtc gcgacgtcgg cgacgaaccg gtcccgctcg acgaggagga
     4381 gtaccagccc ggcgacgtct cgcagcggga tccgccgccc gacccgagct ggggcaacgc
     4441 tggcccgatg gacgtcggcg acttccacct cacgccgaag tgctgcaagt caggcgtcgg
     4501 cgtgtccatc gagtacgaca aggacggcac ccgagtgaag ggcacgctcg aggtggtggg
     4561 gacgctgccc cgtgcctcgt ggcacctgcg gatcagcggc gggacgctgg agtccgccgg
     4621 actcaccctg accggttcgg cgggcatcca ctacgtgttc tccgcggcca caaagaccgg
     4681 gctcgtcggc aacctgcacc gctacgtcca gctcccggcc tcgctggagt ggaaccaggt
     4741 gatcggcggg atcccgttcg cgttcggcct gacccagtcg ttcatcatcc gcaccgcgtt
     4801 cagctccaag gacaaccacc tcgacgcgga gggcctgtgg tcgttcagcg gcggcctcgg
     4861 cttcgcctac acgagcgcca cggggctgca ggtcacgaag ccggccgacg tgtccaccac
     4921 gcggagcatg ctgacgtcga tgggcggcgg gggcaccggc gcagagggga tcgtcctcgc
     4981 ccatcgcctc gaggcacgca tcgggatcgg cgtcacgaac ttctcggtcg gcccgttcgt
     5041 ggacctggtg tcctcggtgg gcgcctctcg cggctcagac atcggcatcc tgatctgcaa
     5101 gggggtcaac ctgaacatcg ccacgggtgc cgggatcagc taccgccttc ccgagatcgt
     5161 ggtgaagatc gtgaacttcg tcctgaagct gttccaggcg aagccgatcc tcagccaggg
     5221 caatatcgcc cgggtcgaga tcccggtcct gaacaggtct gcggtcttcc ctgagtccaa
     5281 gatctgccgc tgacgagccg cgggtggggt ggccgctatc ggtcgcgatc cgcgctcgaa
     5341 ccaggcgcac ctggccgccg tgccccagtt ggcatcacgg caggagcgtt tcgtccacca
     5401 gctggtcgac ggtcaccggg ttcgggacga ggccgagcgt tgtcatgaac tccagcgact
     5461 gctgccaccc gtctcgatcg atcgcgccgt aggcgtcggt ccgcgggttc tgccaggttg
     5521 cgatcgtggc gtccaggatc tggcgctgga gctcgggatc cttggccagg tcgggcacgg
     5581 ccgcgaacgt cgcgtcgagg cccttctggg gatcggcggc gatctcgtcc atcgcgttga
     5641 gggtcacgag ggtgaacgcc ccgagggcgt cgcgcttggt ctcgagcgtc gccttcccgg
     5701 tcacgagacc ggggccgggc agcgggacga tgtcgtcgac cgtgaggacc acgggctcga
     5761 tgccggcctt ccgcagctgg atcggctcgt tgttcgcgaa cccggtggcc gcatcgacgg
     5821 cgccctgctg gagcgcggcc gcctgaccga agtccgggaa ctcgacgatg tcgacatcgg
     5881 ccggcgtgag cccacccgac tgcaggagcg cctggagcat gatccacgag gagccgtact
     5941 tgccggggat gccgatcttc ttgcctcgca ggtcggcggc cttcgtgatg cccgaatcgg
     6001 ccctggccag cacgatcgac gggaacttgc cgtagatcgt cgcggtgtag acgaccggga
     6061 tgccctggct gacggccggg atcaccgagg tgccgtcgcc gctcccgatg tcgaccgcgc
     6121 cctggccgag gagcgtgatg aggtcggggt cgatcttgtt ctggagcgtg acgtcgaggc
     6181 cggcgtccgc gtaggcgccg gactgctggg cgaggtagaa ctgggcgaac tggacgttgg
     6241 ggatgtagcc gaggccgacc gtgagggcaa cgggcgcggc ggagggttcg gtcgattcgt
     6301 tgggcgcgac cgattccggc tgggccgatg gggttccggg cgatcgcgat gcgccggtgc
     6361 cgcaggcggc gacgacgaag gcgagggcgg cgatgagcgt ggctcgggcg gtggctggga
     6421 acgggcgcac gggtcgggtg acctccagga ggtgtgcttg gggtttcatc ggtcgccgac
     6481 caggcgacgt tcgatgagga ccatgaccag atacagggcg accccgatca gcgccagggt
     6541 cagcagggtg gcgaacatga gcggaatgtc gaacaagctg ccccgcgcga tgttgatgag
     6601 cacgcccagg ccggactcgc cgccggccca ctcggcgacg attgcgccga tcaccgcgag
     6661 cgtggcgccg acccgcatcc cgccgaggat cgagggcagc gcggccggga tctcgatgcg
     6721 ggtcgcgatc tggcggcccg tcgcccggaa gctgcgcgcc atctcgatca ggccggggtc
     6781 cacggtccgg atcccgacca tcgtcgcgat cgcgaccggg aagaacacga tcagcgtcgt
     6841 gatcacgagc ttggacagga gcccggtgcc gaaccacagg acgagcagcg gcgccagggc
     6901 gaggatcggg gtcgactgcg cggcgacgag gtaagggctg acgagccgct cgacgaggcg
     6961 ggaacgggcg agtgcgaggc cggcgatcag cgcgagcccg gatccgatcg cgaatccgag
     7021 caggatctcg actagcgtcg tcgccgcgtg gcggcccatc gtcccgtcgg tccatgccgt
     7081 gacgaagcgc tgcgcgacgg tgagcgggcc cggcaggatg aacgacgggt agccggtcag
     7141 gaacacgatc agctgccaga cgagcacgaa cagggcgagg ctgacgacga ccgggatcgc
     7201 gagctcgagc cagcggcgcc ggcgggcggg ccgttcagcc gatccctgcg tggccaggct
     7261 cgagggcacc gcgcggctca cgactcgccg tccatggcaa ggtgggatcg gacctgtgcg
     7321 gcgatctcgg cggctctcgc cgagtccacg tcggccatcc ggcgcggtcg ctgcaatccg
     7381 accggcacgt cggccacgat gcgtcccggg cgcggcgaca tgacgacgac gcggtcggcg
     7441 aggaacaccg cctcgctgat gctgtgggtc acgagcacga tcgtcgtgcc gatgcgctgc
     7501 caca
//
LOCUS       DBNHXO010000136         2217 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2015433, whole genome shotgun sequence.
ACCESSION   DBNHXO010000136 DBNHXO010000000
VERSION     DBNHXO010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2015433"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /gene="aspS"
                     /locus_tag="AC162A_02835"
     CDS             complement(<1..953)
                     /gene="aspS"
                     /locus_tag="AC162A_02835"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR5009801.1"
                     /translation="MPAEASSLATPYRTHTCGALRPADAGTAASLAGWVHRRRDYGKL
                     TFIDLRDRHGITQVVIDAGDAPAASEIAARLRPEFVVAFEGEVALRQPGTENPKLPTG
                     GVELQARTVRILNEAKTPPFYVNDPDAPIDEALRLRYRYLDIRREPMASRLLLRSRLV
                     QAIREVHHANGFVEVETPNLIKSTPEGARDFIVPSRLQPGTVYALPQSPQQLKQLLMV
                     GGIDRYFQIARCFRDEDLRGDRQPEFTQLDLEMSFVDEATVTRFVEQMVIDVTRATVP
                     ERPIRAVPFPVFDYAEVMERYGNDKPDIRFAMELFDLAPALS"
     gene            1070..>2217
                     /locus_tag="AC162A_02840"
     CDS             1070..>2217
                     /locus_tag="AC162A_02840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HIR5009802.1"
                     /translation="MADRIDLLGVLRDGLPGRAAEPRHVVVVGAGIAGLTAALLLKEA
                     GHRVTILEARNRLGGRIYTYRGFAGRMYGEFGAMRFPKQHHLAQHLIHDRFGLATTPF
                     GMEDPDTFVYLHGRRVRRGEFTPESFEFGLPPGERGRDPAALLHDAMQPLIDLLAQPG
                     GWEALIERYDRYSLLGFLIERGLSEPAISLLGPLFNLEGRFHFSLVEWFSHWHEDVFG
                     DLEYIDDGADTLANAFAPALLNDTRLGAEVHAIEQGPDGVVVRYRDNVGTRGTVTADE
                     CVLTVPMVLLRHMEIDGLDVDKWFTIRNVYYGRAHKIFMQFSRRWWVDDYAITHGVTV
                     TDLAIRNVVYTPAGQDPAIDKGVIIASYAWEQDSMAYSMLDESQRIAQA"
BASE COUNT          318 a          778 c          782 g          339 t
ORIGIN      
        1 gacagcgccg gcgccaggtc gaacagctcc atcgcgaacc ggatgtccgg cttgtcgttc
       61 ccgtaccgct ccatcacctc ggcgtagtcg aacaccggga agggcacggc gcggatcggc
      121 cgttcgggca ccgtcgcgcg ggtgacgtcg atgaccatct gttcgacgaa acgcgtgacc
      181 gtcgcctcgt cgacgaagct catctcgagg tcgagctggg tgaactcggg ctggcggtcg
      241 ccgcgcaggt cctcatcccg gaagcagcgg gcgatctgga agtagcggtc gatgccgccg
      301 accatgagca gctgcttgag ctgctgcggg ctctgcggga gcgcatagac cgtgcccggc
      361 tggaggcggc tggggacgat gaagtcgcgc gccccctcgg gcgtgctctt gatcaggttt
      421 ggtgtctcga cctcgacgaa gccgttggcg tggtggacct cgcggatcgc ctggacgagc
      481 cggctgcgga gcagcaggcg actcgccatc ggctcgcgcc ggatgtcgag gtagcggtag
      541 cggagccgga gggcctcgtc gatgggtgcg tccggatcgt tgacgtagaa cggcggcgtc
      601 ttcgcctcgt tcaggatccg taccgttcgg gcctggagct cgaccccacc ggtcgggagc
      661 ttcgggttct ccgtcccggg ctggcgcaac gccacctcgc cctcgaacgc gacgacgaac
      721 tccgggcgca gccgggctgc gatctcgctc gctgcgggcg cgtcgccggc atcgatcacg
      781 acctgggtga tgccgtgccg gtcgcggagg tcgatgaagg tcagcttccc gtagtcgcgc
      841 ctgcggtgga cccagccggc gaggctggcc gccgtcccgg cgtccgcggg tcgcagcgcg
      901 ccacaggtgt gcgtgcggta cggcgtggcg agcgaggagg cttcggcggg cacggcgaac
      961 ggactccgga ctgcggctgc gacgggtccc tatcgtatgt cacgtccctg cccgcggcgg
     1021 tcgcgcaacc aggctccagc cgcggtcgcg ctcgggtacc gtggcgcgaa tggcggaccg
     1081 catcgacctc ctcggggtcc tccgcgacgg ccttcccggg cgcgccgccg agccgcgcca
     1141 cgtggtggtc gtcggcgccg gcatcgcggg cctgacggcg gcgctgctgc tcaaggaggc
     1201 cggccaccgg gtcacgatcc tcgaggcgcg caaccgcctc ggcgggcgga tctacaccta
     1261 ccgagggttc gcgggccgga tgtacggcga gttcggcgcg atgcgcttcc ccaagcagca
     1321 ccacctcgcc cagcacctga tccacgaccg gttcggcctt gccacgacgc cgttcgggat
     1381 ggaggatccg gacaccttcg tctacctcca cggccgccgg gtgcggcgcg gcgagttcac
     1441 gccggagagc ttcgagttcg ggctgccgcc cggcgagcgc gggcgcgacc cggcagccct
     1501 cctgcacgac gcgatgcagc ccctgatcga cctgctcgcg cagccgggcg gctgggaggc
     1561 gctgatcgag cgctacgacc gatactcgct gctcggcttc ctgatcgagc gcgggctcag
     1621 cgagccggcg atctcgctcc tcggcccgct gttcaacctc gagggccgct tccacttctc
     1681 gctggtcgag tggttctcgc actggcacga ggacgtgttc ggggacctcg agtacatcga
     1741 cgacggcgcc gacaccctcg cgaacgcctt cgcaccggcg ctgctcaacg acacccggct
     1801 gggcgccgag gtccacgcga tcgagcaagg ccccgacggg gtcgtcgtcc gctatcggga
     1861 caacgtcggc acccggggca ccgtgaccgc cgacgagtgc gtgctgaccg tccccatggt
     1921 gctgctccgg cacatggaga tcgacggcct cgacgtcgac aagtggttca cgatccgcaa
     1981 cgtctactac ggccgcgccc acaagatctt catgcagttc agccgccgct ggtgggtcga
     2041 cgactacgcg atcacccacg gcgtcacggt caccgacctc gcgatccgca acgtggtcta
     2101 cacgccggcg ggccaggacc cggcgatcga caagggcgtg atcatcgcgt cctacgcctg
     2161 ggagcaggac agcatggcct acagcatgct cgacgagagc cagcgcatcg cgcaggc
//
LOCUS       DBNHXO010000137         2051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3603381, whole genome shotgun sequence.
ACCESSION   DBNHXO010000137 DBNHXO010000000
VERSION     DBNHXO010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3603381"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="AC162A_02845"
     CDS             <1..1164
                     /locus_tag="AC162A_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR5009803.1"
                     /translation="PVVRQSPTSVPIYQTATFSSADAEELAAVLTEEQQGYAYSRIDN
                     PTVVALGDAVAELHGAEAGIALATGMAAIHATFLSILQAGDRLLVGQVGYGTTRTQAA
                     SAFGRLGIDVAYVDTTDVGAVEAALAAHPTRILHVETIANPTCVVADLPELAEVAHRH
                     GALLTVDNTFASPAVCRPLEHGADLVIESGTKYLSGHSDVMAGAVVGPRDRVAAVRSA
                     QIATGATLGPFAAFLVLRGIATVAVRVERQSRTAEALATFLEGRDGVARVTYPGLPSH
                     PQRAAAQRILDTGGAMLSVDLAGGREAGRVFFDALEIPERTASLGSVHTMVVHPPTSS
                     HRTLDAASLAAAGITEGLLRVSVGLEDEADLIADFGQALEAARAASPTPVATG"
     gene            1294..>2051
                     /locus_tag="AC162A_02850"
     CDS             1294..>2051
                     /locus_tag="AC162A_02850"
                     /inference="COORDINATES: protein motif:HMM:NF016995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis ResB family protein"
                     /protein_id="HIR5009804.1"
                     /translation="MLALMAVIGMTLRQLPGFAFRSPTDYANEMDRLHGIYDATLTTG
                     VVDLLERLQLFRVFSSIWFSVGLVVLILSIVACTIDRLPRLWRQSADIRVVQPDPYYD
                     PTLPDRAALAGVSADATASILRRHRFVVRREVVDGTTYLYGDRHRWTKMATLLTHTGL
                     VLFLVAAAVTSRLGDEQGLVVAEGDSLTVQPIGTPGLLLVRNLGFDAPGFATGQPTDF
                     TTHLAVYQDGRQVADKVIRVNDPLAIGGYTFHQNG"
BASE COUNT          270 a          793 c          693 g          295 t
ORIGIN      
        1 cccgtcgtgc gccagtcgcc cacctcggtc ccgatctacc agacggcaac gttctcctcc
       61 gccgatgccg aggagctcgc cgcggtgctc accgaggagc agcaaggcta cgcatacagc
      121 cgcatcgaca acccgaccgt cgtcgcgctc ggcgacgcgg tcgcggagct gcacggggcc
      181 gaggccggca tcgcactcgc gaccggcatg gccgccatcc acgccacctt cctgtcgatc
      241 ctccaggcgg gcgaccggct gctcgtcggc caggtcggct acgggaccac gcggacgcag
      301 gcggcatcgg ccttcgggcg cctcgggatc gacgtcgcct acgtggacac gacggacgtc
      361 ggtgcggtcg aggcggcgct ggccgcacac ccgacgcgga tcctccacgt cgagaccatc
      421 gccaacccga cctgcgtggt cgccgacctc ccggaactgg cggaggtcgc ccaccgtcat
      481 ggggccttgc tcaccgtcga caacaccttc gcctcgccgg ccgtctgccg cccgctcgag
      541 cacggggcgg acctcgtgat cgagtcgggg accaagtacc tctccggcca ctccgacgtc
      601 atggccggcg cggtcgtcgg accgcgcgat cgcgtcgccg ccgttcgctc ggcccagatc
      661 gccaccggcg cgaccctggg gccgttcgcc gccttcctcg tgctgcgcgg gatcgccacc
      721 gtcgcggtcc gtgtcgagcg ccaatcgcgc accgccgagg ccctggcgac gttcctcgag
      781 ggacgggacg gcgtggcgcg ggtgacctac cccgggctgc cgagccaccc gcaacgggcc
      841 gccgcgcaac ggatcctcga cacgggcggc gcgatgctgt ccgtggacct cgccggtggc
      901 cgcgaggccg gccgcgtgtt cttcgacgcc ctcgagatcc cggagcgcac cgcgagcctc
      961 ggcagcgtcc acacgatggt cgtccacccc ccgacgtcgt cccaccgcac gctcgacgcg
     1021 gcgtcgctcg cggcggccgg catcaccgag ggcctgctgc gggtgtcggt cgggctggag
     1081 gacgaggcgg acctgatcgc cgacttcggg caggcgctcg aggccgcgcg cgcggcgagc
     1141 ccgacgccgg tcgccaccgg gtaggaggcg gccatcgcga ccatcgccgc ccggcggccg
     1201 gccgcgcgtc ccgcgccgag cctcccggtc cggatcgcca acggcatctg gggcctgctg
     1261 acctcggtcg acttcgccgt cgtccagatc agcgtgctcg cgctgatggc cgtgatcggg
     1321 atgacgctgc gccagctgcc gggcttcgcc tttcgttcgc cgaccgacta cgccaacgag
     1381 atggaccggc tccacgggat ctacgacgcg acactgacca cgggcgtcgt ggatctgctc
     1441 gagcggctgc agctgttccg ggtgttcagc tcgatctggt tcagcgtggg cctggtcgtc
     1501 ctgatcctct cgatcgtcgc gtgcaccatc gaccggctgc ccaggctgtg gcgccagtcg
     1561 gccgacatcc gcgtcgtgca gcccgacccc tactacgacc ccacgcttcc cgatcgtgcg
     1621 gccctcgccg gggtatccgc ggacgccacg gcctcgatcc tgcgccgcca ccggttcgtg
     1681 gtccggaggg aggtcgtcga cggcacgacc tacctgtatg gcgaccgtca ccgctggacg
     1741 aagatggcga cgctgctcac gcacacgggg ctcgtgctgt tcctcgtcgc cgcggcggtg
     1801 acctcgcggc tcggtgacga gcagggcctg gtcgtcgccg agggcgattc gctcacggtc
     1861 cagccgatcg gaacgccggg cctcctgctc gtgcgcaacc tcggcttcga cgcgccaggg
     1921 ttcgccaccg gccagccgac ggacttcacg acccacctcg cggtctacca ggacggcagg
     1981 caggtcgccg acaaggtcat ccgcgtcaac gacccgctcg cgatcggcgg ctacacgttc
     2041 caccagaacg g
//
LOCUS       DBNHXO010000138         2561 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2015846, whole genome shotgun sequence.
ACCESSION   DBNHXO010000138 DBNHXO010000000
VERSION     DBNHXO010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2015846"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="AC162A_02855"
     CDS             <1..291
                     /locus_tag="AC162A_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIR5009805.1"
                     /translation="AVKGRFGRKSSFAAPDETKALTGHEIGGVTVFGLPADLPIWVDA
                     AVMARDRIVLGGGSRSWKVIAPSSILRTLPNVEVVDGLANPAPPRDEASPGA"
     gene            517..1962
                     /locus_tag="AC162A_02860"
     CDS             517..1962
                     /locus_tag="AC162A_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009806.1"
                     /translation="MALLLGLAPTPAAALQPPRPLPHYHPRFVTQTDERPMNDCLWAS
                     AAMLIDKWTNGKIHPTHQRLRALSGDHVKGSNFDDLKVATRKLGLNFKFSPNGGDRIS
                     FSGLLRRLAHGAGAVVLGDDSRLPRWYGRWDYGFWQMSRKDRKKHPSRDNHAVYVERF
                     DRRHGRVWLMDPLGRAGWKGEWISIWSLRRFAWSSGGALFVAVTPTAKPAPFHHVRTS
                     KATLTQTSAALEANWSFRAPRKWRFPGVSTKTSFSVAKDPLLAAATSTEVAERVTDES
                     RPSHTVVASAGRSLTVKAPLPSKAGTYYGSIKLSDRRFGRTVAQVGSVPVFVPGPRNA
                     SLELRADDDTAEAGKRIEASVSVENTGELTWAETPTDPDDPDPEARNARLVARWIALD
                     VDLKLGPRTDADGNIQPAVPPPTTLDAVPLDAGRHVVVDAPLRAPWVIGRWALVIDVV
                     DDIDGSYARLGSKPAVLIVDVVAPKGRVEID"
     gene            2015..2443
                     /locus_tag="AC162A_02865"
     CDS             2015..2443
                     /locus_tag="AC162A_02865"
                     /inference="COORDINATES: protein motif:HMM:NF020397.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HIR5009807.1"
                     /translation="MTEPIPPEAFLADYAPPIRELAQRLRTIVVGTVPDVVERVRPGW
                     RLIGYDAPDGRRTTYFAWVFPEPVHVHLGFVEGVLMDDIGRHLQGAGETKKARWLTWR
                     PGDDVDADLVAGLVREGLRVAAMSKGERFALAMSLADDPG"
BASE COUNT          373 a          921 c          899 g          368 t
ORIGIN      
        1 gcggtcaagg gacgcttcgg ccgcaagtcg tcgttcgccg cgccggacga gacgaaggcg
       61 ctgaccgggc acgagatcgg cggcgtcacg gtgttcgggc tgccggccga cctgccgatc
      121 tgggtcgacg cggcggtcat ggcccgggac cgaatcgtcc tcggcggcgg cagccgcagc
      181 tggaaggtga tcgctccgtc gtcgatcctg cgcacgctgc ccaatgtcga ggtggtcgac
      241 ggcctggcca acccggcgcc gccgcgcgac gaggcatcac cgggggcctg agcgccgttt
      301 gaccgccgac tgatcgcgcg cgccccacgg gaccatcggg gcaccgactt cgggaccgcc
      361 gagtcatggt gatcccgacg ttccgcggcg aggctgtggg caaccccgcg gtcaacgagg
      421 tcccggcagg cgccggcgga ctgggccgcc gcagcgcagc caggcatgcc caacgtctct
      481 cgacctctcc gagcgctcgc cgctgccccc ctcgtcatgg cgttgctgct ggggctcgcg
      541 ccaacgccag cggccgccct gcagccgccg cggcccctgc cccactacca cccgcggttc
      601 gtcacccaga ccgacgagcg gccgatgaac gactgcctct gggcttcggc cgcgatgctg
      661 atcgacaagt ggaccaacgg caagatccac ccgacgcacc agcggctccg cgcgctgtcg
      721 ggcgaccacg tcaagggctc caacttcgac gacctcaagg tggcgacgag gaagctcggg
      781 ctcaacttca agttctcacc gaacggcggt gaccggatct cgttcagcgg cctcctccgg
      841 cgcctcgccc acggggcggg agccgtcgtc ctcggcgacg actcgcggct cccgcgctgg
      901 tacgggcgct gggattacgg cttctggcag atgtccagga aggaccgcaa gaagcacccg
      961 tcgcgcgaca accacgccgt ctacgtcgag cgcttcgatc gacgccacgg tcgcgtgtgg
     1021 ctcatggacc cgctcggccg tgcgggctgg aagggcgagt ggatcagcat ctggtcgctg
     1081 cgccggttcg catggagcag cggtggcgcc ctgttcgtcg ccgtcacccc gaccgcgaag
     1141 cccgccccgt tccaccacgt cagaaccagc aaggcgacgc tgacccagac ctctgccgcc
     1201 ctcgaggcga actggtcgtt ccgggctccc cggaagtggc gtttccccgg cgtgtcgacg
     1261 aagacgtcgt tcagcgtggc caaggatccg ctgctcgccg ccgcgacgtc caccgaggtc
     1321 gccgagcgcg tgaccgacga gagccggccg tcgcacacgg tggtcgcatc ggcgggacgg
     1381 tcgctcacgg tcaaggcgcc gttgccgagc aaggccggca cctactacgg gtcgatcaag
     1441 ctcagcgacc gtcgcttcgg ccggaccgtc gcccaggttg gcagcgtccc cgtgttcgtt
     1501 ccgggaccac gcaacgcgtc cctcgagctg cgcgccgatg acgacacggc ggaggctggg
     1561 aagcgcatcg aggcgtccgt gtccgtcgag aacaccggcg agctgacgtg ggccgagacg
     1621 cccaccgatc cggacgatcc ggaccccgag gcgcggaacg cgcggctcgt cgcgcgctgg
     1681 atcgcgctcg acgtcgatct caagctgggt ccacggacgg atgccgacgg caatatccag
     1741 ccggccgtgc ccccgcccac gaccctcgac gcggtgccac tcgacgccgg caggcatgtt
     1801 gtcgtcgatg cgccgctgcg cgcaccgtgg gtgatcgggc gctgggcgct cgtgatcgac
     1861 gtggtcgacg acatcgacgg gtcgtacgcg cgcctcggca gcaagccggc ggtgctgatc
     1921 gtcgacgtcg tcgcgcccaa gggccgggtg gagatcgact agctccggcg cgtacctgtc
     1981 aggtcctggc cgccgccggt gcgaccatgc ggacatgacc gagcccatcc cgcccgaggc
     2041 gttcctcgcc gactacgcgc cgccgatccg cgagctcgct cagcgcctgc gaacgatcgt
     2101 cgtcgggacg gtgcccgacg tcgtcgaacg cgtccgtccc ggctggcggt tgattggcta
     2161 tgacgctccg gacggtcgcc gcacgacgta cttcgcatgg gtctttccgg agccggtcca
     2221 cgtccacctc ggattcgtcg agggggtcct gatggatgac atcggtcgcc acctgcaggg
     2281 agcgggcgag acgaagaagg cccgctggct cacttggcga cccggcgacg acgtcgatgc
     2341 cgacctggtc gcggggctcg tccgcgaggg cctgcgggtg gcggcaatgt cgaagggcga
     2401 gcggttcgcg ctggcgatgt cgctggcgga cgatccgggc tgacgggtgg tgccgccgtg
     2461 caccgggaag gcgcacaatc gcggctcacg ttcaccggag gctgcgcatg gcgacggact
     2521 tcacgatcgg cctcgtcgac cgtccgggaa cgctcgcccg g
//
LOCUS       DBNHXO010000139         2685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2611115, whole genome shotgun sequence.
ACCESSION   DBNHXO010000139 DBNHXO010000000
VERSION     DBNHXO010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2611115"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..770
                     /locus_tag="AC162A_02870"
     CDS             66..770
                     /locus_tag="AC162A_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015746712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009808.1"
                     /translation="MEVIGWFTDPANWSGTSGIPNRMLEHLVLAGLAILIGCLIAIPV
                     GLFIGHTGKGAGAAVAIGNLGRAIPSYALLVIFVPLLGIGFFNALAALVLLSLPPILT
                     NTYAGLQGVDRDLVEAGRGMGMRERQILRGVELPLALPVILAGVRIASVQVVATATLA
                     ALVAGGALGRFIVDGFAQQNTPMLVGGAILVALLAILTERFMTILEHRATSPGLRAVL
                     DAGSERPAEVAAPTPS"
     gene            904..1932
                     /locus_tag="AC162A_02875"
     CDS             904..1932
                     /locus_tag="AC162A_02875"
                     /inference="COORDINATES: protein motif:HMM:NF015995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR5009809.1"
                     /translation="MRMTRMAALGATSLVLLVSACASGGSSSTPAATSAGASTPASVA
                     PASEPASEPASAGPSIASQLVLGGPPECPTRPFCQQGLEKTYGITFKEFKPLDVAGPL
                     TVEALKNGDVQVGMLLTSDPAYATNGFVLLQDDKKLQLADNLVPVLSKAIVDANPDVA
                     TLLNSATAKVTQSELTALNKAVTVDGQTSAEAATQWLTDNELLPATAVPSRGQDITVG
                     SANFYEQEVLAEVFAQVLEANGFTVERKFQLGAREVVFPALEKGDIDLLVEYAATALE
                     YVNKSAGEATTDATANVAKLNEHLAALGLVALEPASATDQNGFAVTTDTATQYSLTNL
                     SDLANPAP"
     gene            complement(1996..>2685)
                     /locus_tag="AC162A_02880"
     CDS             complement(1996..>2685)
                     /locus_tag="AC162A_02880"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR5009810.1"
                     /translation="VRAHDLGRRTTISRSAILGTIVIERVVDTFVVVAIASIAILVLS
                     VRGIVASAVLAGLAVTALLVVGVAAGIAAHRLPGASRVAAFLDRWPRIRAALTRLREG
                     LAVASNARVVGIAVILSLASWSCTVLAFAAAAQAVGVEPTIGQAALLAAGTNLATAIP
                     AGPGYVGTFELAVITIAGSVGIDQTSALAFGLLVHLATLLVTSIGGAIVLIFGGRGRR
                     PEADEEAAPAG"
BASE COUNT          412 a          977 c          937 g          359 t
ORIGIN      
        1 gccgacggcg gcctcgggtt cgccgggcgc ctgctcacgc cgtgggctcg tcggggcagt
       61 cgctgatgga ggtcatcggc tggttcaccg atcccgcgaa ctggtcgggg acgagcggca
      121 tcccgaatcg gatgctggag cacctcgtgc tcgccggcct cgccatcctc atcggctgcc
      181 tgatcgcgat cccggtcggg ctgttcatcg ggcacaccgg caagggcgcg ggcgccgccg
      241 tggccatagg caacctgggc cgcgcgatcc cgtcctacgc gctgctcgtg attttcgtcc
      301 cgctgctcgg gatcgggttc ttcaacgcgc tggcggcgct ggtgctgctg tcgctgccgc
      361 cgatcctcac caacacctat gccggccttc agggcgtcga ccgggacctg gtcgaggccg
      421 gccgcggcat gggtatgcgg gagcgccaga tcctgcgcgg ggtcgagctg ccgctggcgt
      481 tgccggtgat cctcgccggg gtccgcatcg cgtccgtgca ggtggtggcg acggccacgc
      541 tcgcggccct ggtggccggc ggggcgctgg gccggttcat cgtcgacggc ttcgcccagc
      601 agaacacgcc gatgctcgtc ggcggggcga tcctggtcgc gttgcttgcg atcctgaccg
      661 agcggttcat gacgatcctc gagcaccgcg cgacgtcgcc cggactgcgt gccgtcctcg
      721 acgcgggctc ggagcgaccg gcggaggtcg cggctcccac gccgagctga ccgtcacgcg
      781 gttgtaacgc gggggacccc gagtcgaacc gaattcctgt caggtccgaa cacgaacagc
      841 aggtagcatc tcggggcccg atcaccgcgc gttgcggtgc gggagtcaca gaaggaggac
      901 ccaatgcgga tgacccgcat ggctgccctc ggggcgacgt cgctcgtgct gctcgtcagc
      961 gcgtgcgcgt cgggtgggag cagctcaaca ccggccgcca cctcggccgg cgcctcgaca
     1021 ccggccagcg ttgcgccggc gagcgagccc gccagcgagc ccgcgagcgc gggcccgtcg
     1081 atcgccagcc agctcgtgct gggtggcccc cccgaatgtc caacgcgtcc gttctgccag
     1141 caggggctgg agaagacgta cgggatcacg ttcaaggagt tcaagccgct cgacgtggcc
     1201 ggacccctga ccgtcgaggc gctcaagaac ggcgacgtcc aggtcggcat gctgctgacc
     1261 tcggaccccg cgtacgcgac caacggcttc gtcctgctcc aggacgacaa gaagctccag
     1321 ctcgcggaca acctggtgcc ggttctgagc aaggcgattg tcgatgccaa tcccgatgtc
     1381 gcgaccctgc tcaactcggc gaccgcgaag gtcacccaga gcgagctgac ggccctcaac
     1441 aaggcggtca ccgtcgatgg ccagacctcc gcggaggccg cgacgcagtg gctcaccgac
     1501 aacgagctcc tgccggcgac ggccgtccca agccggggcc aggacatcac cgtcgggtcc
     1561 gcgaacttct acgagcagga ggtcctggct gaagtgttcg cccaggtcct cgaggccaac
     1621 ggcttcaccg tcgagcgcaa gttccagctc ggcgcccgcg aggtcgtgtt cccggcgctc
     1681 gagaaggggg acatcgacct cctcgtcgag tatgcggcga cggcgctcga gtacgtgaac
     1741 aagagtgccg gcgaggcgac caccgacgcg acggcgaacg tcgccaagct caacgagcac
     1801 ctcgcggccc tcggcctcgt cgcgctcgag ccggcgtcag ccaccgatca gaacgggttc
     1861 gccgtcacca cggacacggc gacccagtac tccctcacca acctcagcga cttggcgaat
     1921 ccggcgccgt aacgcgcccg ggtcaacgac cctcgccacg caccgaggcc ccggcaccgc
     1981 cggggccttt cgctgtcacc cggccggcgc ggcctcctcg tccgcctcgg gccgccgccc
     2041 gcgaccgccg aagatcagca cgatcgcgcc gccgatcgag gtcaccagca gcgtcgccag
     2101 gtggaccagc agcccgaacg caagggcgga cgtctggtcg atgccgaccg accccgcgat
     2161 cgtgatcacc gcaagctcga acgtgccgac gtagcccggc cccgctggga tcgccgtcgc
     2221 gaggttcgtg cctgcggcca gcaacgcggc ctgcccgatc gtcggctcga cgcccaccgc
     2281 ctgggccgcg gcggcgaacg cgagcaccgt gcacgaccag gacgcgagcg acaggatcac
     2341 cgcgatgccg acgacccgcg cgttgctggc gacggcgagg ccctcgcgca ggcgggtcag
     2401 cgcggcgcgg atccgcggcc agcggtcgag gaacgcggcc acccgggatg cccccggcaa
     2461 ccggtgcgcg gcgatgccgg ccgcgacacc gacgacgagc agggcggtca ccgcgagccc
     2521 cgcgagcacc gccgaggcga cgattccgcg cacggacagc acgaggatcg cgatcgaggc
     2581 gatggcgacg acgacgaacg tgtcgaccac gcgctcgatg acgatggtgc cgaggatcgc
     2641 cgatcgcgag atcgtggtgc ggcggcccag gtcgtgggcc cggac
//
LOCUS       DBNHXO010000140         3695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2216472, whole genome shotgun sequence.
ACCESSION   DBNHXO010000140 DBNHXO010000000
VERSION     DBNHXO010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2216472"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="AC162A_02885"
     CDS             <1..263
                     /locus_tag="AC162A_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009811.1"
                     /translation="IRVAPVNVEVRTDWFSGTPREVTWGADRMQVTRIVAVRDERSAY
                     PAITGPRTLFEVDTASFRLALTYRHRSRRWTIEGLDEAPKAA"
     gene            complement(359..1672)
                     /locus_tag="AC162A_02890"
     CDS             complement(359..1672)
                     /locus_tag="AC162A_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009812.1"
                     /translation="MLIVDGPVSADTYDWYEVQVADDGLFGWVAAGKGSEDWIEPAEP
                     RCTDDLQESVLWTRDPIDFLVCFGVEPVNVEVRAPDPGFDGVTGGIPSCSYTGGDVPC
                     AARPRWLFDVIPFSFTAPEAPAEPLFVAATGSMAARLEDVPDLATLTLTLAMDSLEAQ
                     DCRVVDDNGRDLISRDEAITRCRLTLVVRDVAWDPATAGEYGPNAMARVEVDNLGVRS
                     RPGLGPESRLLPKTLAAGSTVYIDAGPVSADGIDWYAVLWTDMESLYGWVPSIEVGAP
                     TLTPLETECAPMKDWPAFVNLSFKERLVCYGDQPITIRLRVRVTQDLSPDWSFACNWV
                     NNSTVSLATPCVAEPRWLASFSGIVGIRPGEMEVMLAFDPTRLKRGDFPADYTWKVVT
                     GTFAHPASASCRAIDPGTGADLLPPAEAAIYCRSQFVVTKLEPVP"
     gene            complement(2176..2760)
                     /locus_tag="AC162A_02895"
     CDS             complement(2176..2760)
                     /locus_tag="AC162A_02895"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5009813.1"
                     /translation="MEPVTIRVTDVIRLDDQGLIQRARSGDAEAFDAIAARRIPDAYR
                     LASAILGSQAAAADATQNALLAAWRELPRLRDVDAFDAWFHRILVNECRMQVRHQTRL
                     RETELTESGPDAAASHPQGKDASTLELVEVLDLLEGAFERLEPDDRAMVVLHHLEGRP
                     LTEIAALLHMPVGTVKWRLHQARGVLQHALEAVE"
     gene            2913..3581
                     /locus_tag="AC162A_02900"
     CDS             2913..3581
                     /locus_tag="AC162A_02900"
                     /inference="COORDINATES: protein motif:HMM:NF016825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HIR5009814.1"
                     /translation="METTSRFRDLTVAAFVDQLSSAEPVPGGGSAAAVAASLAAALVS
                     MVAQLSEGRPKYAEHAALHAEASAAAKALADRFLTLADEDAEAYAGFGAAMRLPRDTD
                     EERDARDAAMRNAAREASDVPYRTVEACLEVVVLAEALAGRSNRNASSDLEVAALLAT
                     AASRAAAANVYVNLPSIGDESAAGELLADTRRLVDDIDRLASQTRETVLGGEARPPIE
                     AGRG"
BASE COUNT          547 a         1312 c         1311 g          525 t
ORIGIN      
        1 tgatcagagt cgctccggtc aacgtagagg tccgcacgga ctggttcagc ggcacgcccc
       61 gagaggtgac ctggggcgcc gaccgcatgc aggtcacgcg catcgtcgcg gtccgcgacg
      121 agcggtctgc ctaccccgcg atcacgggtc cgcggacgct gttcgaggtc gacacggcct
      181 cgttccgcct cgccctgacg tatcgccacc ggtcccgccg gtggacgatc gaggggctcg
      241 acgaggcgcc gaaggccgcc tgaccccgca ccacactcgg ggtcgcggct cctccccgtc
      301 ggccccacat ggcggctgcg gggcgggcgc ggcagcgctc gccccgcggg ccactttgtt
      361 acggaaccgg ctcgagcttc gtcacgacga actgcgagcg gcagtagatc gcggcctccg
      421 cgggcggcag caggtcggca ccggtgcccg ggtcgatggc gcggcaactc gccgaggccg
      481 gatgcgcgaa cgtgccagta accaccttcc aggtgtagtc ggcggggaag tcgccacgtt
      541 tcagccgcgt tggatcgaag gccagcatca cctccatctc ccctggtcgg atgccgacga
      601 tgccggagaa ggatgcgagc cacctgggct ccgcgacgca tggagtggcc aacgagaccg
      661 tcgagttgtt gacccagttg caggcgaagc tccagtcagg actgaggtcc tgtgtcaccc
      721 gtacccgtag cctgatggtg atgggttgat cgccgtagca aacgagccgt tccttgaagg
      781 acaggttcac gaacgccggc cagtccttca tcggagcgca ttcggtttcg agcggcgtca
      841 gggtgggtgc ccccacctcg atcgagggaa cccagccgta caggctctcc atgtccgtcc
      901 acaggacggc gtaccaatcg atgccgtctg cgctcacggg ccccgcgtcg atgtagacgg
      961 tgctgccggc ggcgagtgtc ttcgggagca ggcgcgattc aggaccgagg cccggccgag
     1021 accgaactcc gaggttgtcg acttcgaccc gcgccatggc gtttggcccg tactcgcctg
     1081 ctgtcgcggg gtcccaagca acgtcgcgta ccacaagggt caggcggcac cgcgtaatcg
     1141 cctcgtcgcg cgaaatcagg tctctgccgt tgtcgtcgac gactcggcag tcttgcgctt
     1201 cgagggaatc catcgcaagg gtcagcgtca gcgtcgcgag gtcgggcaca tcctcgagtc
     1261 gggccgccat ggaacccgtg gcggccacaa acaggggttc ggccggcgct tccggtgccg
     1321 tgaacgagaa cgggatgaca tcaaacagcc agcgcgggcg tgctgcgcat gggacgtcgc
     1381 cgccggtgta cgaacacgaa ggaatgcctc cggtcacgcc atcgaaacct ggatcgggag
     1441 cccgcacctc gacgttcacg ggctcgacgc cgaagcacac gaggaagtcg atcgggtcgc
     1501 gcgtccacag gaccgactcc tggaggtcat cggtgcagcg gggttccgcg ggctcgatcc
     1561 agtcctcgct gcccttgccg gcagccaccc agccgaatag gccgtcgtcg gctacctgga
     1621 cctcgtacca gtcgtaggtg tcggcggaga ccgggccgtc gacgatgagc atccgcgcgc
     1681 ccttcttcag cagcggcgag aacttctcgg agtccgcacc gacgctcggc cgggacctga
     1741 cgcgcaggcc gtcgcccgcg ttcgtcacca cggcgatcgt gtcgggcgcg agggccgggg
     1801 gcgccggcgt cggcaccgcc tgcggctcgt tcgttggttc cttcctcggc tcgggtgtgg
     1861 cgggcgacag cgtggcgctc ggcgagggaa gcaccacgac tgcgggcagc gtcggctgcg
     1921 gtggcggcag gatcagccgg gcggcgacca ggaccccggc cgcgagtgcc aggagcacaa
     1981 gcgctgcggc cgcgacgagg cccatgcgcc cgaagcgccg ggtactcagc gcgccgctcc
     2041 aggcatgtcc gccgcggacc tggccactct ggcgaaccgc ctcagccact ccgggcagca
     2101 gccagccggg cgggggtgcg cccgcccggc gctcgagcat caggcgcagc aggtcttcgg
     2161 tgcgatcggg cgtcgtcatt cgaccgcctc cagggcgtgc tgcaggaccc cgcgggcctg
     2221 gtgcagccgc cacttcacgg ttccgacggg catgtgcaac agggccgcga tctcggtcag
     2281 cggccggccc tcgaggtggt ggaggacgac catcgcgcgg tcgtcgggtt ccagccgctc
     2341 gaaggcaccc tcgaggaggt cgagcacctc gacgagctcg agggtcgagg cgtccttgcc
     2401 ctgcgggtgc gaggcggccg catcaggccc ggactcggtg agctcggtct cgcgcaggcg
     2461 cgtctggtgg cgcacctgca tccgacactc gttgaccagg atccggtgga accaggcgtc
     2521 gaaggcatcg acgtcgcgca gccggggcag ctcgcgccaa gccgccagga gcgcgttctg
     2581 ggtcgcgtcc gcggccgccg cctggctgcc caggatggcc gacgccagcc ggtatgcgtc
     2641 ggggatccgg cgtgcagcga tggcgtcgaa ggcctcggcg tccccggaac gcgcgcgctg
     2701 gatgagcccc tggtcgtcga gccggatcac gtccgtgacc cgaatggtga cgggctccat
     2761 ccctcgctcc aatcctgcat gcacatgatg ccgaccgggg ctgttcggtt ggaagacgcc
     2821 tttcgacgga ttctgtcgcg tgcgaggcgc ggatcggccc ggcacccgca cccgcccaac
     2881 cgaccgagac ggcgcctccc tacaatcgag ccgtggaaac cacctcccga ttccgcgacc
     2941 tcacggtcgc cgcgttcgtc gaccagctct catcggccga acccgttccg gggggtggca
     3001 gcgccgccgc cgtggcggcg agcctcgcgg ccgccctcgt gtcgatggtc gcgcagttgt
     3061 ccgagggccg gccgaagtac gccgaacatg ccgccctgca cgcggaggcg tccgcggcgg
     3121 cgaaggcgct cgccgatcgg ttcctgaccc tggccgacga ggacgccgag gcctacgcgg
     3181 gtttcggggc cgcgatgcgc ctcccgcgcg acaccgacga ggaacgcgac gcgcgagacg
     3241 ccgccatgcg caatgcggcg cgggaagcct ccgacgtccc gtaccgcacg gtcgaggcgt
     3301 gtctcgaggt cgtcgtcctc gccgaggcgc tcgccgggcg gagcaacagg aacgcgtcgt
     3361 cggacctcga ggtcgcggcg ctcctcgcga cggccgcatc gagggccgcc gccgcgaacg
     3421 tctacgtgaa cctgccctcg atcggggacg agtccgcagc cggcgagctc ctcgcggaca
     3481 cccggcggct cgtcgacgac atcgaccgcc tggcatcgca gacccgcgag acggtgctgg
     3541 gcggcgaggc tcgcccgccg atcgaagccg ggcgcgggtg atcgcgcgat gaccgacggc
     3601 ccgcggctcc tgctcggcgc gccgatcgcc gcggagatcc gcgccaccgt cgcccgcgac
     3661 gtcgacgcga tgcgcggccg tcgcggccac gcccc
//
LOCUS       DBNHXO010000141         3315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2611713, whole genome shotgun sequence.
ACCESSION   DBNHXO010000141 DBNHXO010000000
VERSION     DBNHXO010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2611713"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="AC162A_02905"
     CDS             <1..311
                     /locus_tag="AC162A_02905"
                     /inference="COORDINATES: protein motif:HMM:NF037014.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009815.1"
                     /translation="TDGSRVIVDPVDSHHRLTIDAGWTSREVLVPVFRGGERVYDPPP
                     LAEARDHARHELGTLHPAILRQLRPHWYPAGLEWGLHTRREALIEAELAPDIAGAPAG
                     "
     gene            complement(318..1208)
                     /locus_tag="AC162A_02910"
     CDS             complement(318..1208)
                     /locus_tag="AC162A_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR5009816.1"
                     /translation="MNDAPGELGFVHGASWLHRLSPIPKLAWLTSVVVFAFVSFNAAI
                     LASIVIVGLVLATSAGILAHAVRALALLAPLAAAILLLQSIAPAVCGGACTPVASLGP
                     LTIYEEGVARGLTFVLRILAMETAAILVLQTTHPSDLFAALARLRVPYLLNVMIALTL
                     RLVPVLQREVRIVPAAQRSRGMRGTGLAAVVPALGPVFAGTVDRVGQLAISLESRGFG
                     ASGPRTSYRRIGFSTRDLVLAVTGVAAGIAAVVATIALGPTASPAFVVPAGAAVALVA
                     AALVVFAGSLVRAAMAIGRA"
     gene            complement(1205..2068)
                     /locus_tag="AC162A_02915"
     CDS             complement(1205..2068)
                     /locus_tag="AC162A_02915"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR5009817.1"
                     /translation="MARGRRVSEAPSPIVEVRNVSFRYGRNGPPVLEHLDVEVPRGQV
                     VALAGRNGSGKSTLARLLNGLLRPTTGSVVVGGLDTSRNPPRRMAAHAGYAFQNPNHQ
                     LFASTVAAELAFGPRNLGVAEAEIAERVADAAARLGLEGVLDRHPYHLERGARKLVAI
                     AAVVTMRTPVLILDEPTTGQDRRTSAMVRALIDDLGAGGTTIVCISHDMRLVADAADR
                     LLVLDDGRIVADGTPRAVFGDLSAIARAGLAPPQITRLALRLPKRAEPWPALTVDELA
                     GAVRHGAADAS"
     gene            complement(2047..2889)
                     /locus_tag="AC162A_02920"
     CDS             complement(2047..2889)
                     /locus_tag="AC162A_02920"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR5009818.1"
                     /translation="MAGAGVSIRGLWFTHAHAPGPALRDISLEIAPGELVGIVGRNGS
                     GKSTLCLALNGVVPHLLPGERIGSVVVGGIDPATTTVREMARVVAMVFDDPDAQVSQT
                     TVADEVAFGLENLGVPWGEMDARIGSALTAAGLAGLEGRDPATLSGGEQQRLAIACAI
                     AMRPSVLVMDEPTANLDPAGRAAVFEIAARLHRDDGMTVIIAEHDVEALAEHASRVIV
                     LDAGAVVMDGPPAEVFGRIAGASDDGVAVPEVTSLAARLGERPGRPMPVTVDDAVAWL
                     GDAG"
     gene            complement(2905..>3315)
                     /locus_tag="AC162A_02925"
     CDS             complement(2905..>3315)
                     /locus_tag="AC162A_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009819.1"
                     /translation="YGVRTFGMGRNPATYFVLCVIVGIATGAAGAVIALFVYGGATGH
                     PSDVVTAALVAAGGALVNAVFTSSILTSIADKVISGFVAIAIIGALPPRYTHGLPLPA
                     EVGTRTLLVATVGTVAGIAILLVYLVILAPPAAA"
BASE COUNT          497 a         1228 c         1210 g          380 t
ORIGIN      
        1 caacggacgg ctcccgggtg atcgtggatc ccgtggactc ccaccaccgg ctgaccatcg
       61 acgccgggtg gacctcgcgc gaggtcctgg tgcccgtgtt ccgcggcggc gaacgcgtct
      121 acgacccgcc gccacttgcc gaggcgcgtg accacgcccg ccacgagctg ggcacgctgc
      181 atcccgcgat cctgcgccag ctgcggcccc actggtaccc ggccggtctc gagtggggcc
      241 tgcacacgcg ccgcgaggcc ctgatcgagg ccgagctcgc ccccgacatc gccggggctc
      301 ccgccggctg acccgcctca ggctcggccg atcgccatcg cggcccgcac gagagagccg
      361 gcgaacacga cgagggcggc cgcgacgaga gcgacggccg cgccggccgg caccacgaac
      421 gcgggcgagg cggtgggccc cagcgcgatg gtcgcgacca cagcggcgat ccccgccgcc
      481 acccccgtca ctgccaggac gaggtcgcgc gtggagaagc cgatccgtcg gtacgaggtg
      541 cgcgggccgc tcgctccaaa gccgcgcgac tcgaggctga ttgccagctg tccgacacgg
      601 tcgaccgtgc cggcaaacac gggcccgagt gccggcacca cggccgcgag ccccgtgccg
      661 cgcatccccc tcgatcgctg cgccgccggc acgatccgca cctcgcgctg gaggaccggg
      721 acgagccgca acgtgagggc gatcatgacg ttgaggaggt acggaacccg cagccgggcc
      781 agcgcggcga acaggtccga cggatgcgtc gtctgcagga cgaggatcgc cgcggtctcc
      841 atcgccagga tgcgcaggac gaacgtgagg ccgcgcgcca cgccctcctc gtagatcgtc
      901 agcgggccga ggctcgcgac cggggtgcat gcgcccccgc agacggcagg cgcgatcgat
      961 tgcaggagca ggatcgcggc ggcaagcggc gccagcaggg cgagcgcccg cacggcatgc
     1021 gccaggatcc ccgcgctcgt ggcgagcacc aagccgacga tgacgatcga cgccaggatc
     1081 gcggcgttga acgacacgaa cgcgaacacg acgacggacg tgagccaggc gagcttcggg
     1141 atggggctca ggcggtgcag ccaggatgcg ccgtggacga agcccagctc ccccggcgcg
     1201 tcgttcacga cgcgtccgcc gcgccgtggc ggacggcgcc tgcgagctcg tccaccgtga
     1261 gcgccggcca gggctcggcg cgcttgggca gccgcagcgc gagccgcgtg atctgcggcg
     1321 gcgcgaggcc cgcccgggcg atggcggaga ggtcgccgaa cacggcgcgc ggcgtcccgt
     1381 cggcgacgat ccggccgtcg tcgagcacga gcagccggtc ggcggcgtcg gcgacgagcc
     1441 gcatgtcgtg cgagatgcac acgatcgtcg tcccgccggc gccgagatcg tcgatgaggg
     1501 cgcggaccat cgcggacgtt cgccggtcct ggccggtcgt cggctcgtcg aggatgagga
     1561 cgggcgtgcg catcgtcacg accgcggcga tggcgacgag cttccgtgcg ccccgctcga
     1621 ggtggtacgg atggcgatcg aggacgccct cgaggccaag ccgggcggcg gcgtcggcga
     1681 cgcgctcagc gatctcggcc tcggcgacgc cgagattccg gggcccgaac gccagctcgg
     1741 cggcgacggt cgaggcgaac agctggtggt tcgggttctg gaacgcatag ccggcgtggg
     1801 cggccatgcg tcggggcgga ttccgggacg tgtcgaggcc gccgacgacc accgagccgg
     1861 tcgtcggtcg caggaggccg ttgaggagac gggcgagggt cgacttgccc gacccattcc
     1921 tgccggcgag cgccaccacc tgcccgcggg gcacctcgac gtcgaggtgc tcgaggaccg
     1981 ggggtccgtt gcggccgtag cgaaacgaga cgttccgcac ctcgacgatc gggctcggcg
     2041 cctcgctcac ccggcgtccc cgagccacgc cacggcatcg tccacggtga ccggcatcgg
     2101 ccggccgggg cgctctccca gccgcgcggc gagcgacgtc acctccggga ccgcgacgcc
     2161 gtcgtcgctt gctcccgcga tgcgcccgaa cacctccgcc gggggcccat ccatcacgac
     2221 tgcaccggcg tcgaggacga tgacccggct cgcgtgctcg gccagtgcct cgacgtcgtg
     2281 ctcggcgatg atcacggtca tcccgtcgtc gcggtggagg cgggccgcga tctcgaacac
     2341 ggccgcccgc ccggcgggat cgaggttggc ggtcggttcg tccatcacga ggaccgacgg
     2401 ccgcatcgcg atcgcgcagg cgatcgccag gcgctgctgc tcgccgcccg agagggtggc
     2461 cggatcgcgc ccttccagcc cggccaggcc ggccgcggtc agcgcggacc cgatccgtgc
     2521 gtccatctcg ccccacggca cgcccaggtt ctcgagaccg aaggccacct cgtcggcgac
     2581 cgtggtctgg ctcacctggg catcggggtc gtcgaacacc atcgccacga cccgggccat
     2641 ctcgcggacg gtcgtcgtgg cggggtcgat gcccccgaca accacgctgc cgatgcgctc
     2701 ccccgggagc aggtgcggca ccacgccgtt cagcgcgagg cagagggtcg acttgccgga
     2761 cccattgcgt cccacgatgc cgacgagctc gcccggcgcg atctccagcg agatatcgcg
     2821 cagcgcgggg cccggcgcgt gcgcgtgcgt gaaccacagc ccgcggatgg agacgccggc
     2881 gccggccatc gacgagcgcg cggctcaggc cgcggcgggc ggggcgagga tcaccaggta
     2941 caccagcagg atcgcgatgc ccgcaaccgt gcccacggtc gcgaccagca gcgtccgcgt
     3001 cccgacctcg gccggcagcg gcagcccatg ggtataccgg ggcggcagcg ccccgatgat
     3061 cgcgatggcg acaaacccgc tgatcacctt gtccgcgatc gaggtcagga tgctcgacgt
     3121 gaacacggcg ttgaccagcg cgccaccggc ggcaacgagc gcggccgtga caacgtccga
     3181 gggatggccc gtcgcaccgc catacacgaa cagcgcgatg accgccccag cggccccggt
     3241 ggcgatcccg acgatcacgc acagcacgaa gtacgtggcc gggttccggc ccatgccgaa
     3301 ggtccggacg ccata
//
LOCUS       DBNHXO010000142         2319 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3803871, whole genome shotgun sequence.
ACCESSION   DBNHXO010000142 DBNHXO010000000
VERSION     DBNHXO010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3803871"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1142)
                     /locus_tag="AC162A_02930"
     CDS             complement(24..1142)
                     /locus_tag="AC162A_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnose ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5009820.1"
                     /translation="MKYTSTGRLVALIGGAAIALSACSSTGGATSAPSAAAPTAAPTP
                     AATVEAPSTPASAPASQAAGPVTVGYLPKDIVNQYFAAAKVGIDKAAGELGGTVTQVG
                     PNEAKADLQIPFITDLTTQGVSAIIISADGKDEVAPALKAAMDKGIKVVGFDSSPAVG
                     AYNVFVNQADFSGVGTSLVEWACEIAPSCTGEIAILSAAATATNQNTWIEGMKTTLAD
                     PKYAGLKLVDTVYGDDDATKSTQQAQALLTKYPDLKVIVAPTTVGVLAAAQEVSQQKL
                     SDKVKVTGLGFPNQMKPFVKDGTSPVFGLWSVPDLGYLSYVVADKLIKGEITGAEGET
                     FSVPGLNNDQPYTIGKDSVVTLGPAFRFDGTNVDQFNF"
     gene            complement(1160..2212)
                     /locus_tag="AC162A_02935"
     CDS             complement(1160..2212)
                     /locus_tag="AC162A_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009821.1"
                     /translation="MTPSTASGTTGRSLVRLLARWETLLLVVLIVMIVIGSQLSKFFL
                     TPGNFANLLAALMEVAIMALPMTLIIIAGEIDLSVESMAGLSSAVLGFLWAAGVPLPI
                     AVLLVIVMGALGGLMNGLFVARGGLPSLVVTLGTLALFRGLALIVLGPRGVSNFPPEF
                     TTLGFGYVPGTLIPWPFVIFIALAIILGIVLHRTWAGRQVYAVGKSTGAARFAGVRVQ
                     RLRISLFVLTGLIAALAGVILTSRLSSARADAGQGMTLTVVTVVLLGGVNIFGGRGTI
                     PGVVLAVLVVAVMQNALRLASVTVEVQSIALGLLLILSVVIPSFAHQAKSAIDRARRG
                     RPPPTDVVPRGEQAVT"
BASE COUNT          377 a          757 c          843 g          342 t
ORIGIN      
        1 cccggggccg gctgaaccga tgactagaag ttgaactggt ccacgttggt gccgtcgaac
       61 cggaacgccg gcccgagcgt cacgacgctg tccttgccga tcgtgtacgg ctggtcgttg
      121 ttgaggcccg gcacgctgaa ggtctcgccc tcggcgcccg tgatctcgcc cttgatcagc
      181 ttgtcggcca cgacgtagga gaggtagccg aggtcgggca cgctccacag gccgaacacc
      241 ggggacgtgc catccttgac gaacggcttc atctggttcg ggaagccgag gccggtgacc
      301 ttgaccttgt ccgacagctt ctgctgggac acttcctggg cggccgccag gacgccgacg
      361 gtcgtcggcg ccacgatgac cttgaggtcc gggtacttcg tgagcagcgc ctgtgcctgc
      421 tgggtgctct tggtcgcgtc gtcatcgccg tagacggtgt cgacgagctt gagccccgcg
      481 tacttgggat cggcgagcgt ggtcttcatg ccctcgatcc aggtgttctg gttcgtcgcg
      541 gtggccgcgg cggagaggat ggcgatctcg cccgtgcagc tcggggcgat ctcgcacgcc
      601 cactcgacga ggctggtgcc gacgccgctg aagtccgcct ggttgacgaa cacgttgtag
      661 gcgccgaccg ccgggctgga gtcgaagccg accaccttga tgcccttgtc catggcggcc
      721 ttgagtgcgg gcgcgacctc gtccttgccg tcggccgaga tgatgatcgc gctcacgccc
      781 tgggtcgtga ggtcggtgat gaacgggatc tggaggtcgg ccttggcctc gttggggccg
      841 acctgggtga ccgtgccacc gagctcgccg gcggccttgt cgatgccgac cttcgcggcg
      901 gcgaagtact ggttcacgat gtccttcggc aggtagccga cggtgaccgg gcccgcggcc
      961 tgggaggccg gggccgatgc cggcgtcgac ggtgcctcga ccgtggctgc cggcgtcggc
     1021 gcggcagtcg gcgcggcggc cgacggggcg gacgtggcgc cgcccgtgct cgagcatgcg
     1081 gagagcgcga tggccgcgcc cccgatgagg gcgaccagtc gccccgtgga tgtgtacttc
     1141 aaggctgttc ctccacctgc taagtgactg cttgctcacc acgtgggacc acgtccgtcg
     1201 gcggcggccg acctcggcgg gcgcgatcga tggccgactt ggcctggtga gcaaaggaag
     1261 ggatgacgac cgagaggatc agcagcagcc cgagggcgat gctctggacc tcgacggtca
     1321 cgctcgcgag ccgcagcgcg ttctgcatca cggccacgac gagcacggcg agcacgacgc
     1381 ccgggatggt cccgcggccg ccgaagatgt tgaccccgcc gagcagcacg acggtgacca
     1441 cggtcagggt catgccctga ccggcatccg cccgggcgct cgacaggcgc gacgtcagga
     1501 tcacgcccgc gagggcggcg atgaggccgg tgaggacgaa caggctgatc cgcagccgct
     1561 ggacgcgaac gccggcgaac cgggcggcgc ccgtgctctt gccgaccgcg tacacctggc
     1621 ggccggccca ggtccggtgc aggacgatgc ccaggatgat ggcgagggcg atgaagatca
     1681 cgaacggcca cgggatcagc gtcccgggga catagccgaa gccgagggtg gtgaactcgg
     1741 gcgggaagtt gctcacgccc cgcggcccga ggacgatcag ggcgagaccg cggaacaggg
     1801 cgagggtgcc cagggtgacg acgagcgacg gcaggccgcc gcgggccacg aacaacccgt
     1861 tcatcagccc acccagggcg cccatcacga tgaccagcag cacggcgatg gggagcggca
     1921 cgccggcggc ccacaggaac ccgagcacgg cactcgacag cccggccatc gactcgacgg
     1981 agaggtcgat ctcgccggcg atgatgatca gcgtcatcgg caacgccatg atcgccactt
     2041 ccatgagcgc ggcgagcagg ttcgcgaagt tgccgggcgt caggaagaac ttcgacagct
     2101 gcgatccgat gacgatcatc acgatcagca cgaccagcag cagcgtctcc cagcgcgcga
     2161 gcagccggac caggctgcgt ccagtggtgc cggaggcggt cgagggggtc atcggctgcg
     2221 actcgctgcc gcaaggcgct ggacccggcg attgatcagt gcatcggcgc tgacggcgaa
     2281 caggatcacg acgccgtaga tcgcctggag ccagaactg
//
LOCUS       DBNHXO010000143         3962 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1819655, whole genome shotgun sequence.
ACCESSION   DBNHXO010000143 DBNHXO010000000
VERSION     DBNHXO010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1819655"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="AC162A_02940"
     CDS             <1..431
                     /locus_tag="AC162A_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5009822.1"
                     /translation="GLAMIDAARQEGDGIAGLRLRVGVHSGPVIGGVIGHRKFAFDIW
                     GETVNIASRLETHGVPGRVHVSASTRGNVADLFEVEPRGPMQLRGYGPMETYTIARRR
                     TSESEDPRHSPEAPLTTTPAYRPGRARRVAITPLATSGGA"
     gene            complement(535..810)
                     /locus_tag="AC162A_02945"
     CDS             complement(535..810)
                     /locus_tag="AC162A_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009823.1"
                     /translation="MDAGATGRASGDHGRGRRYRRHELALPDGGKLRLGVDGRIERLD
                     ADGSTERSWTVADPDWPNQAIRFGLRPQPPTVTPRGQRIPDLGLPGA"
     gene            complement(815..1102)
                     /locus_tag="AC162A_02950"
     CDS             complement(815..1102)
                     /locus_tag="AC162A_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009824.1"
                     /translation="MARTSFSSPASSIVVCSDGYFDVYPPVDRSNGDKPAYRGELGEL
                     GAGIRGIDDRLAVRPDSAALAGAVEAWSAAYGSAAVRGELGHGRDGKATDG"
     gene            complement(1393..3309)
                     /locus_tag="AC162A_02955"
     CDS             complement(1393..3309)
                     /locus_tag="AC162A_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009825.1"
                     /translation="MLAPGEVIVEGGRLRIGHAGIPNVAYPDGPFVPGETVTATITGV
                     VARGRFAGKPYLEHTGRGELLGSATVSATVPLTWLTDEFAELVFDPPVSIRYHAPTSA
                     ALACERNQGKIAYLMVGVIADFTGSTGTEARIDTTSDLPLASVIEPEVRCPVRSDFDG
                     DGYADLAIGAPGDRAGTVDLAGAVNVLYGSAAGLTADGDQLWTQDSRGVPGVSRTREQ
                     FGAALAAGDFDRDGKADLAVGAPSDSIDGLRSGGVTILYGTKRGLRATGSQRWSRANL
                     PGSPASGDQFGAAVAAADFDGDGYDDLVVGVPADDIAADGDDLGSIEVLYGGPAGLEA
                     GRATNFDRSYLGATERLSGFFGRALAAGDLDGDGYADIAIGAPLGNTGVDDVGILYGG
                     PLGLTTEGSQSWSQASPGVAGDTDRDDWFGAALSAGDFDLDGIDDLAIGAPGDDVLGN
                     GRHQGGVTVLYGSPEGLIAQGSQWWHQNLPNFPGSAENYDWFGQAVSAGNLDNDGYAD
                     LAIGSPGDRVGSADGAGAIFVLWGGSGGLTRFGAQRWSQASAGMPGTPEAGDRFGSSL
                     AIGHFGRSAPGDLAIGIPGEAIGARGDDGLIGVLYGRTNSLWSKHSQEWWQDRMGIGG
                     SSENHDGLGTALTP"
     gene            complement(3655..3731)
                     /locus_tag="AC162A_02960"
     tRNA            complement(3655..3731)
                     /locus_tag="AC162A_02960"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3695..3697),aa:Arg,seq:tcg)
BASE COUNT          581 a         1418 c         1372 g          591 t
ORIGIN      
        1 tcgggctcgc gatgatcgac gccgccaggc aggagggcga cggcatcgcc ggccttcggc
       61 ttcgagtcgg cgtgcactcc gggccggtca tcggcggcgt gatcggccac cgcaagttcg
      121 cgttcgacat ctggggcgag accgtgaaca tcgccagccg gctggagacg catggcgtcc
      181 ctggccgcgt ccacgtcagc gcctcgacac gggggaacgt ggccgacctg ttcgaggtgg
      241 agccccgcgg tcctatgcag ctccgcggct acggtcccat ggagacgtac acgatcgcca
      301 ggcgccgcac cagcgagagc gaggacccgc gccacagccc ggaagcccca ctgaccacaa
      361 cgcccgcgta ccgccccggc cgggcacgac gcgtcgcgat cacgccgctc gcgacatccg
      421 gcggcgcctg aacggcggtc ggcgcggtcg tccacgcggc ggtggtcctg gcgcgcccgc
      481 gcgatgagcc gatccggtgc ctcgatgccc tcgctgaagc cgggcccggc gcgtctacgc
      541 tcccggcagc cccaggtccg ggatgcgctg gccgcgaggc gtgacggtgg gtggctgggg
      601 gcgcaacccg aaccggatcg cctggttggg ccaatcgggg tcggccaccg tccaggaacg
      661 ctcggtcgat ccgtccgcat cgaggcgttc gatcctgccg tcgacgccga gcctgagctt
      721 gccgccgtcg ggcagcgcga gctcgtgacg ccggtagcgt cgcccccgtc cgtggtcgcc
      781 gctcgcgcga ccggtggcgc cagcgtccac gtgcctagcc atcggtcgcc ttgccgtccc
      841 gtccgtggcc gagctccccc cgaactgcgg cgctgccgta ggcggcggac caggcctcga
      901 ccgcgcccgc gagcgccgcg gagtccggcc ggacggcaag gcgatcatcg atgccacgga
      961 tccccgcgcc gagctcgcca agttcgcctc ggtaggcggg cttgtcgccg ttcgatcggt
     1021 cgaccggcgg atacacgtcg aagtagccgt cgctgcacac gacgatgctc gaggcaggcg
     1081 aggagaagga ggtacgggcc acaggaggaa tccgatccgt cgcgcgcttc ggaccggcga
     1141 aggagagccc ggcgcaattg gcgactgcgc cacctaccat cggctctcgg ctcgattccg
     1201 tcaaacgagg tggatcggga ccctggccgc gcgggccgcg agaggacctg gcgcgcccgg
     1261 agggcgccaa gcgcgcagga acaaagcagg cgagtcgatt gggcgctgcg caacgcgcgg
     1321 cttgttgtcg atcggcccgg cgattgcggg ttacaccgtc gatcccgatc gcaagcgccc
     1381 gctggtccca ggctaggggg tcagcgcggt accaaggcca tcgtggtttt cggagctgcc
     1441 cccgatgccc atccggtcct gccaccactc ctgcgagtgc ttggaccaga ggctgttagt
     1501 ccggccatac aggacgccga tgagcccgtc gtcgccacgc gctccgatcg cctctcccgg
     1561 gatcccgatg gcgaggtcgc cgggcgcgga ccgcccgaag tggccgatcg caaggctgga
     1621 gccgaatcgg tcgcccgctt cgggcgtccc gggcatgccc gcgctcgcct gcgaccagcg
     1681 ttgggcacca aatctggtaa ggccgcctga tccgccccat aggacgaaga tggctcccgc
     1741 gccatcggcg ctcccaacgc ggtcaccggg agagccgatg gcgaggtcgg cgtagccgtc
     1801 gttgtcgagg ttccccgcgg acaccgcttg tccgaaccag tcgtagttct ccgcggagcc
     1861 cggaaagttc gggaggttct gatgccacca ctggctgccc tgtgcgatca ggccctcggg
     1921 gctcccgtac agcacagtga cgccaccctg gtggcggcca ttgccgagga catcgtcgcc
     1981 aggcgccccg atcgcgaggt cgtcgatgcc gtccagatcg aagtcgccgg ccgagagtgc
     2041 agcgccaaac cagtcatccc ggtccgtatc gcccgctacc cccggactcg cctggctcca
     2101 ggactggctg ccctccgtcg tcaggccgag tgggccccca tagaggatcc ctacatcatc
     2161 gacgccggtg ttgccgagcg gcgcgccgat cgcgatgtct gcgtagccgt cgccatcaag
     2221 atcgccggcg gcgagtgccc ggccaaagaa cccggataga cgctcagttg ctcccagata
     2281 gctacggtcg aagttcgttg cacgtcctgc ctcgagtccc gcaggcccgc cgtacaggac
     2341 ctcgatcgag ccgagatcgt caccgtcggc cgcaatgtcg tccgcgggca ccccgacgac
     2401 gaggtcgtcg tagccgtcgc cgtcgaagtc agcggctgcg acggcggccc cgaactggtc
     2461 gccgctagcc ggtgagcccg gcaggttcgc gcggctccag cgctgactgc ccgtggcccg
     2521 caggccgcgt ttcgtgccgt agaggatggt tactccgcct gaccgcaggc cgtcgatgct
     2581 atcgctcggc gcgccgactg cgaggtctgc cttgccgtcg cggtcgaagt ccccggcagc
     2641 gagcgcagcc ccgaactgct cacgcgtccg gctcacgccg ggaacccctc gactgtcttg
     2701 cgtccagagt tggtcgccgt cggccgtcaa gccggcggcc gagccgtaca ggacgttcac
     2761 tgcgccagca aggtccaccg ttcccgctcg gtcacccgga gccccgatgg cgaggtcggc
     2821 gtagccatca ccgtcgaagt cggaccgcac cgggcacctg acctcaggct caatgacaga
     2881 tgcgagtgga aggtcggacg tcgtgtcgat ccgggcctcg gtcccggtac tccctgtgaa
     2941 gtcagcaatg actccgacca tcagatacgc gatcttgccc tggtttcgct cgcacgcgag
     3001 ggcggccgac gttggcgcgt ggtaccgaat cgagaccggc gggtcgaaca ccagttcggc
     3061 gaactcgtcg gttagccacg tgagtggcac ggttgccgac accgtggccg accccagtag
     3121 ctcgccccgg cccgtgtgct cgagataggg cttcccggcg aaccgcccgc gggcgacgac
     3181 tccggtgatc gtcgccgtca cggtctcacc cggcacgaac gggccgtccg gataggcgac
     3241 gttggggatg cccgcgtgcc cgattcgcag ccggcccccc tcgacgatga cttcgccggg
     3301 cgcgagcacc cgcatggacc cgaccgagat gttggcttgg tcggcagacg tgatcgttgg
     3361 agccgcaagg aggagtgtcg cgagggcggc agctcggagc gcacgcttgc ggggcgtccg
     3421 tttggcgggg ccgacagcgc cggccgccag gattgggcta cgtcgcccac gagcagtcat
     3481 gtgggcaggc atgaatagtg ctccagggcg ggctccgagg acgccgtcac gcgtccgccc
     3541 tgagacgccc gacgtcgtct cttgtggcgg cttggcacat cgcaggccgt ttcgaggggg
     3601 catcgaagcg cggctcgcgc gtgggtgccc ggttgagcac cgagctagag gacctggcgc
     3661 gcccggaggg attcgaaccc ccgacactcg gttccgaaga ccgatgctct ggtccactga
     3721 gctacgggcg cgcggcgggc gacatcatac ccagcgccgc tcaagttgcg gtggggccgc
     3781 cgggtgcggc acactccgcc ggtgcctcgt cagcatttcc cgacgtcgcg tcctcggcgc
     3841 cggatcggca tcgcgcgggc cgccctcatc ggtgcagccg ctgccgtggt catcgtcctc
     3901 gccctgcccg cgaccacgcc gcggggtgac gtcctcgaca tcgtgaccga ccacgtcggg
     3961 tc
//
LOCUS       DBNHXO010000144         5214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5391732, whole genome shotgun sequence.
ACCESSION   DBNHXO010000144 DBNHXO010000000
VERSION     DBNHXO010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5391732"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..2909
                     /locus_tag="AC162A_02965"
     CDS             252..2909
                     /locus_tag="AC162A_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5009826.1"
                     /translation="MARGSSPTLIGRADDLERLRAALTGARTHGRPVVLVSGEAGIGK
                     SRLLAEFATSATADPLDGRPVRFLHGGCLEVGETLAYLPILEWLEELARDGGEAGTEA
                     RLLHDQFGGSVGPVAIDADTGPGRSTRLQRLRDVVAASAADADVVAVIDDLHWADRST
                     LDVVSFLARRLAGSNVVLVLAFRSDELHRRHPLTPVVAELERHATLDHIRLAPLGSDE
                     VRAQIEAIEGQAADPGDVARVVALADGNPFHVEELLALEGSGPLPDSLRDVLDARLGQ
                     VDDGTLEVLREAAVIGRQVDAELLATISRAGDAAVDDGIRTAIGTRILVADPDGRHYR
                     FRHALLREAVYDDMRPVERIATHRRIAEALTARPDLGDPAPAVALAERAHHWLAARAE
                     PEAFVALIESGRAARGANAWAEGRKAFEEALALWDRVPDPEAAGVARSRLLEDAAAMT
                     WYMGETRAALNLNRRTQTEPDVQADPPRLARLLDHEAWYVWEFGDLDLSRELARQANE
                     LLPADVPTTELALARGTLGAQAQMVGRSRDAVAILEEGLAIARAAGNDGAAVPIHAFL
                     AVAYAELGEPDRALLALRSAEEGMDRVTVEDAFFAVTTNAPWVWVTIGDYEGAVTSSQ
                     WAMTLAEQRGLDIGGGQWVTAPRAEAEYRLGRWDDALATIAGSADYVTAPSPASTQQS
                     ILARILAGRGDYQAARSVVGEALRLARPQAIDESVAARTGETWVEVLGGDPATAAHGL
                     RTAWSAWADADGRFWRSELAWQAAWVATLLAERPGRRDADTGEDLAAFAVEAVGPIRE
                     PGVYVDGIDPKFDLAKAFLARRAGHDDPAIWATAAEQLERVGYVPLAALARQGEAEAH
                     LRAGSADSAEPAIRRALGHVDAMGATAMR"
     gene            3012..3224
                     /locus_tag="AC162A_02970"
     CDS             3012..3224
                     /locus_tag="AC162A_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5009827.1"
                     /translation="MSVREREVLSLVAEGRTNREIGAALFISDKTASVHVTHILTKLG
                     VSSRTEAALLAARAGGDLAEPDPQRN"
     gene            3379..>5214
                     /locus_tag="AC162A_02975"
     CDS             3379..>5214
                     /locus_tag="AC162A_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009828.1"
                     /translation="MIGRSSGGRGPGIGFRAAVVTIGAVLIAACASSTPPSSAPGAGA
                     TPAPSAPGASATASTGAPSGAAEGQTDLDRLVADAVADASPFSLSTADRSLQDTMDEQ
                     TGLTAALGPELHDWLQEQRSAAAVAALEARGIDPASLGPTGISSEPVASTTIGLTAMT
                     GLGWVGTAVVAWAMVQSGGNSIGQSGSGGPQTSQSTSQTTIGDERITASMTFTTQLTI
                     SGSTVIVDADATETMSVSSVSTGAAIGSSTSHNRIHVEANMCPDASGGVPVTVTSDWS
                     EDGSGMPGGSSFSMQSTMTIAATVADDAFLTSSTTTATTNYTASGAGGSQSGSTTRGW
                     SQTMGPSGSSSQGTSGPVTSTVTGNLTVGQGGAMHGLSTILPMKVADQALEVAQAAWR
                     SGKCVEIIASEQSRNVKRNEVIPFTAQARQKIEGVNLTVPVVASFGGVKSIDPVDTPI
                     PGPAHFTFTAGPAKDNVGDVFLKSTSRRGIGTLGLRFRVKVEGWFVDGTFTRGPVSGT
                     SKGKHCGDDPLGDWNVRGTYTVVSGVTIKGKQTWKVKITTEDRPTGIWSGTFTYQDDS
                     SLPVGPVTIQQHTRVSGTAQLTLNDTDGTALMKFTERSRRQWAEVP"
BASE COUNT          764 a         1860 c         1922 g          668 t
ORIGIN      
        1 ggtctgctcg tgctcaaggg cagctccctc ctcacggtcg cgatctccgg catggcggac
       61 ctgtcggagg aggcaggaga ggccgcggcg aaggaggtcg ccgcgctgat cgccgcccgg
      121 atgtgacccg cgggggttcg gtgggcgcgc ccggttggcg cccgcgggat gtggcccgcg
      181 cggcaagacg cacgcgcggg cacggccgca tggactcgac acacgaccgt cgctgggcga
      241 cgatgccggc gatggcgcgc ggatcgagcc ccacgttgat cggcagggcc gacgatctcg
      301 agcggctgcg ggccgcactt acgggcgccc ggacgcacgg ccgacccgtc gtgctcgtca
      361 gcggcgaggc cgggatcggg aagagccggc tgcttgcgga gtttgcgacc tcggcgacgg
      421 cggaccccct ggacggcagg ccagtgcgct tcctgcacgg cggctgcctg gaggtcggcg
      481 agacccttgc ctacctgccg atcctcgagt ggctcgagga gctggcaagg gacggcggcg
      541 aggccgggac cgaggcgcgg ctgctgcacg accagttcgg cggcagcgtc ggacccgtgg
      601 cgatcgatgc ggacacgggc ccggggcgct ccacgcggct ccagcgcctg cgcgatgtcg
      661 tcgcagcatc ggccgccgac gccgatgtgg tagccgtcat cgacgacctc cactgggccg
      721 accggtcgac gctcgacgtg gtctcgttcc tcgcccgccg gctggccggc tcgaacgtcg
      781 tgctggtgct ggccttccgc tccgacgagc tccaccgccg ccacccgttg acgccggtag
      841 tggccgagct cgagcgccat gcgacgctcg accacatccg cctggcgccg ctcggctcgg
      901 atgaggtccg ggcacagatc gaggccatcg agggccaggc cgccgacccg ggcgacgtcg
      961 cgagggtcgt cgcgctcgcg gacggcaacc cgttccacgt cgaggagctg ctcgcgctcg
     1021 agggctccgg tcccctcccc gactcgctgc gtgacgtcct cgacgcgcgc ctcggccagg
     1081 tcgacgacgg gacgctcgag gtcctgcgtg aggcggccgt gatcggccgg caagtcgatg
     1141 cggagctgct cgcgacgatc tcgcgggccg gcgacgcggc ggtcgacgac gggatccgga
     1201 ccgccatcgg cacgcggatc ctcgtggccg acccggacgg gcggcactac cggttccgtc
     1261 acgccctcct ccgcgaggcc gtgtacgacg acatgcggcc ggtcgagcgg atcgccaccc
     1321 atcgccggat cgccgaggcg ctgacggcaa ggcccgacct cggcgatccc gcgccggccg
     1381 tcgccctcgc ggagcgggcc caccactggc tcgccgcgcg cgccgagccc gaggcgttcg
     1441 tggccctcat cgagtccgga cgggcggcgc gtggagccaa cgcctgggcc gagggacgca
     1501 aggcgttcga ggaggcgctt gcgctgtggg accgcgtgcc ggacccggag gcggcaggcg
     1561 tggcccggtc acgcctgctc gaggacgccg ccgcgatgac ctggtacatg ggtgagacac
     1621 gcgcggcact caacctgaac cgccgcacgc agaccgagcc cgacgtccag gccgacccac
     1681 cccgactcgc gaggctcctt gatcacgagg cgtggtacgt gtgggaattc ggcgacctcg
     1741 acctctcccg cgaactcgct cggcaggcca acgagctgct tccggccgac gtccccacca
     1801 cggagctggc cctggcccgc gggacgctcg gggcgcaggc gcagatggtc ggccggtccc
     1861 gcgacgccgt cgcgatcctc gaggaggggc tggcgatcgc ccgggccgcc ggcaacgacg
     1921 gcgcggccgt gcccatccac gccttcctcg cggtcgccta cgcggagctc ggggagcccg
     1981 accgggcgct tctcgccctc cggagcgccg aggaagggat ggaccgggtg acggtcgagg
     2041 acgcgttctt cgccgtgacc acgaacgcgc cctgggtctg ggtcacgatc ggcgactacg
     2101 agggtgcggt tacgtcgagc cagtgggcga tgacgcttgc cgagcaacgc gggctggaca
     2161 tcgggggcgg gcagtgggtg acggcgccgc gcgccgaggc cgagtaccgg ctcgggcgat
     2221 gggacgacgc gctggcgacg atcgcgggat cggcggacta cgtgacggcg ccgagccccg
     2281 cctcgaccca gcagtcgatc ctcgcgcgca tcctggccgg ccgcggcgat tatcaggcgg
     2341 cacggtcggt ggtcggcgag gcactcaggc ttgcccggcc gcaggcgatc gacgagtcgg
     2401 tggcggcgcg gaccggcgag acctgggtcg aggtattggg cggcgacccg gctacggcgg
     2461 cgcacggtct ccgcacggcg tggtcggcat gggcggacgc cgacggccgc ttctggcgct
     2521 ccgagctcgc gtggcaggcc gcctgggtcg ccaccctgct tgcggagcgg cccgggcgac
     2581 gcgacgcgga caccggcgag gacctcgccg ccttcgccgt ggaggcggtc ggccccatcc
     2641 gtgagcccgg ggtgtatgtc gacggcatcg accccaagtt cgacctcgcg aaggcgttcc
     2701 tcgcccggcg tgcggggcac gacgacccgg cgatctgggc gaccgccgcc gagcagctcg
     2761 agcgcgtcgg ctacgtgccg ctggcggccc ttgcgcgcca gggagaggcc gaggcgcacc
     2821 tccgcgccgg atcggccgat tccgccgagc ccgcgatccg gcgggcgctc ggacacgtcg
     2881 acgcgatggg ggcgaccgcg atgcgctagc ccatcgagcg gctggcccgg gcggcacgga
     2941 tcacgctcga tcgggccggt gatgcggcga cgaccgacgg gtcaccatcg ctggccgccg
     3001 atccgtgggg cttgtccgta cgcgagcgcg aggtgctctc gctggttgcc gagggacgga
     3061 ccaaccgcga gatcggcgcg gcgttgttca tcagtgacaa gaccgcgtcg gtgcacgtga
     3121 cccacatcct gaccaagctc ggggtgtcga gccggaccga ggccgcgttg ctcgcggcgc
     3181 gggcgggcgg cgacctcgcg gagccggatc cgcagcgcaa ctagcccttg cgccgcgacc
     3241 gcgggtcgtt acgatgcgac cgccacgctt cggcaccggt tggcgcagct ggacgatgac
     3301 gatgcggcgt ggcaacgggc agattgcctg cgacacggca cccacccaag ggcagccggc
     3361 gcgagcggag gctgccagat gatcggacgg tccagtgggg gccgcgggcc ggggatcggg
     3421 ttccgcgcgg ccgtcgtcac gatcggcgcc gtcctcatcg cagcgtgcgc gtcgtctacg
     3481 ccgccatcat cggcaccagg cgcaggggcc acgccagctc cctcggcacc cggtgcctcg
     3541 gccaccgctt cgacgggcgc cccgtcgggt gccgccgagg gccagaccga cctggaccgc
     3601 ctcgtcgccg acgcggtcgc cgacgcctcg ccgttcagcc tctcgaccgc ggatcgctcc
     3661 ctccaggaca cgatggacga gcagactggt ctgaccgccg cgctcggacc ggagctccac
     3721 gactggctcc aggagcaacg ctcggccgcc gcggtggccg ctctcgaggc ccgtggcatc
     3781 gacccggcct ccctcggacc gacggggatc tcgtcggagc cagtcgcctc gaccaccatc
     3841 ggcctcaccg cgatgaccgg cctcgggtgg gtcggcacgg cggtcgtcgc ctgggcgatg
     3901 gtgcaatcag gcggcaactc gattggccag tccggcagcg gcgggccgca gacgtcacag
     3961 tcgacgagcc agacgacgat cggggatgag cgcatcacgg cctcgatgac gttcaccacc
     4021 cagctcacga tcagcggctc gacggtgatc gtggatgccg atgcgaccga gaccatgagc
     4081 gtgtccagcg tgtcaacggg agcggcaatc ggcagttcca ccagccacaa ccgcatccac
     4141 gtcgaggcca acatgtgccc cgatgcgagc ggtggcgtcc cggtcacggt caccagcgac
     4201 tggtccgagg acggcagcgg gatgcccggc ggcagctcgt tctccatgca gtcgaccatg
     4261 acgatcgccg cgacggtggc cgacgacgcc ttcctcacgt cgtcgaccac gacggccaca
     4321 acgaactaca ccgcgagcgg cgccgggggc tcgcagtccg ggagcacgac caggggctgg
     4381 tcccagacca tgggcccgag cggatcgagc tcgcagggca cgagcggtcc ggtgacgtcc
     4441 actgtgaccg gcaacctcac ggtgggccag ggtggggcga tgcacggcct gtcgacgatc
     4501 ctgccgatga aggtcgccga ccaggcgctg gaggtggccc aggccgcctg gcgtagcggc
     4561 aagtgcgtcg agatcatcgc atcagagcag agtcgcaacg tcaagcgcaa cgaggtgatc
     4621 ccgttcaccg cgcaggccag gcagaagatc gagggcgtga acctcacggt gccggtggtc
     4681 gcgtcgttcg gaggcgtgaa gagcatcgac cccgtcgaca cgcccatccc cggaccggcg
     4741 cacttcacgt tcacggcggg cccggccaag gacaacgtcg gcgacgtctt tctcaagtcg
     4801 acgtcccggc gcggcatcgg gacgctcggc ctcagattca gagtgaaggt agagggctgg
     4861 ttcgtggacg gtacgttcac ccgcggcccg gtcagcggca cctccaaggg caagcactgc
     4921 ggcgacgacc cgctcggcga ctggaacgtg cgcggcacgt acacggtcgt gtccggcgtg
     4981 accatcaagg gcaagcagac ctggaaggtc aagatcacga ccgaggaccg gccgaccggc
     5041 atctggagcg ggacgttcac ctaccaggac gactcgtccc tgcccgtcgg gcccgtcacg
     5101 atccagcagc acacgcgcgt cagcggcacg gcacagctca cgctcaacga caccgacggg
     5161 accgcgctca tgaaattcac ggagcggtcg cggcggcagt gggcagaggt tccc
//
LOCUS       DBNHXO010000145         1762 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3407052, whole genome shotgun sequence.
ACCESSION   DBNHXO010000145 DBNHXO010000000
VERSION     DBNHXO010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3407052"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="AC162A_02980"
     CDS             <1..341
                     /locus_tag="AC162A_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024687.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR5009829.1"
                     /translation="KGITVHLMSCPTVLNEREVSRLIDVEWEAAPAQTYPIAIRVEAY
                     DRTGLLSDITQVMAENKVNILAANVGVNPDHTAVVAMTIQIASVAQLAKVLGRVEQLK
                     DVISAQRDLG"
     gene            complement(393..620)
                     /locus_tag="AC162A_02985"
     CDS             complement(393..620)
                     /locus_tag="AC162A_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009830.1"
                     /translation="MVEARATSVRLTLARRTAAARTCQIIAARRLVAALVTKRHGLAI
                     WRRPANVAQHGVRADPEDSRRRPLDLRTVAI"
     gene            complement(705..>1762)
                     /locus_tag="AC162A_02990"
     CDS             complement(705..>1762)
                     /locus_tag="AC162A_02990"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5009831.1"
                     /translation="IVTQAVKMILAFTMFALVATGAVEVWQVMVLALLGGLTNVVDMP
                     TRQAFAVEMVGREDIGNAVALNSAMFNAARVLGPAVAGLTIGAFDISTAFLIDAISFL
                     AVLVALLAMRQNELRVAPLIARPSSVREVGRDLREGLSYVRRTPLVLMGVLVVGLTAT
                     FGMNFQVVIPPLTEEVLHSDATGYGFLMAASGLGSLVAALYIAFSPRSRVGLIAGGSI
                     LVGIMEIGLGLSGLFFVSLVLMFFVGLGAIAMAATANTTIQLAVPDHLRGRTMSVYTT
                     VFAGSTPVGGLLMGWVASRYGVAEAMALGGAVCLVIGLAAFAWLRRIRADALIAARVT
                     PATLASAGSQASVARPR"
BASE COUNT          318 a          599 c          635 g          210 t
ORIGIN      
        1 gcaaggggat caccgtccac ctgatgtcat gcccgaccgt gctcaacgag cgcgaggtca
       61 gccggctgat cgacgtcgag tgggaggccg ccccggcgca gacctacccg atcgcgatcc
      121 gcgtcgaagc ctacgaccgg acgggactcc tgtcggacat cacccaggtg atggccgaga
      181 acaaggtcaa catcctggcg gccaacgtcg gcgtgaaccc ggaccacacg gcggtcgtcg
      241 cgatgacgat ccagatcgcg tcggtcgcgc agctggccaa ggtcctcgga cgggtcgagc
      301 aattgaagga cgtcatcagc gcccaaagag acttgggctg acgcgccgct gatccgcgcc
      361 cgctgatcct cggccgcggc ggcggcgctc tgtcagatcg ccaccgtgcg cagatcgagt
      421 ggccgtcggc gggagtcctc ggggtcggcc cgcacgccgt gctgggccac attcgcaggc
      481 cgtcgccaga tcgccagccc gtggcgcttc gtcacaagcg cggcgaccaa gcgccgtgcg
      541 gcgatgatct gacaagtgcg tgccgcggcg gtgcgtcgcg cgagcgtgag ccgcaccgac
      601 gtcgcgcgcg cctcgaccat acgccgtcac ccggcgacga tctgacagtc cgagcgagcg
      661 agccggcccg ggggccaggc gaggaggccg gcgagtgggt gtggctagcg ggggcgagcg
      721 acggaggcct gggagccagc tgaagccagc gtcgccggcg tgacgcgggc ggcgatcagc
      781 gcgtcggcac ggatccggcg cagccaggcg aaggcggcga ggccgatgac gaggcacacg
      841 gcgccgccca gggccatcgc ttcggcgacg ccgtagcgtg aagccaccca gcccatcagc
      901 aggccgccaa cgggcgtgga cccggcgaag accgtcgtgt acacgctcat cgtccggcca
      961 cggaggtggt cgggaaccgc gagctggatc gtggtgttgg cggtcgcggc catggcgatc
     1021 gccccgaggc ccacgaagaa catcagcacg agcgacacga agaacaggcc ggacaggccg
     1081 aggccgatct ccatgatgcc gacgaggatc gacccacccg cgatcaggcc cacgcgggac
     1141 cgcggggaga acgcgatgta cagggcggcc acgagcgacc cgaggcccga cgccgccatc
     1201 aggaacccgt agcctgtcgc gtcggagtgg agcacctctt cggtcagcgg cgggatgacc
     1261 acctggaagt tcatgccgaa tgttgcggtg agtccgacca cgaggacgcc catcagcacg
     1321 agcggggtgc gacgcacgta cgacaggccc tcgcgcaggt cgcggccgac ctcgcggacg
     1381 gagctcgggc gcgcgatcag gggcgcgacg cgcagctcgt tctggcgcat cgcgagcagc
     1441 gcgaccagga ccgcgaggaa gctgatggcg tcgatcagga atgcggtcga gatatcgaac
     1501 gcgccgatgg tgaggcccgc cacggccggc ccgaggacgc gcgccgcgtt gaacatcgcc
     1561 gagttgaggg cgaccgcgtt gccgatgtcc tcgcgcccaa ccatctcgac ggcgaacgcc
     1621 tggcgggtcg gcatgtcgac aacattggtc aggccgccca acagggcgag gaccatgacc
     1681 tgccacacct cgactgcgcc ggtcgccacc agggcgaaca tcgtgaaggc caggatcatc
     1741 ttgaccgcct gcgtcacgat ca
//
LOCUS       DBNHXO010000146         4302 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_629962, whole genome shotgun sequence.
ACCESSION   DBNHXO010000146 DBNHXO010000000
VERSION     DBNHXO010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_629962"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="AC162A_02995"
     CDS             <1..652
                     /locus_tag="AC162A_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR5009832.1"
                     /translation="VICTAFRNPALTAKMISTMDVISGGRMELGIGAGWKRDEWDAYG
                     YGFPETRERLARLADDLEVVSRMMDGDRHQHATFEGTYSSVTDARNVPKPVQRPHVPI
                     MVGGNGPNVTWRLAAKFADELNLDGMDPSDVAAALPTVRARCEEIGRDPATLAISVHM
                     WAEDIGLPGQSRVDRLAGLRELGVNRAMGLVRATTTDDEALPRFIEDARAAGVEVG"
     gene            complement(668..1057)
                     /locus_tag="AC162A_03000"
     CDS             complement(668..1057)
                     /locus_tag="AC162A_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5009833.1"
                     /translation="MFESLDYLYTPSRDVAADVRYFTDVLGARLVFAIEAMGTRVAMV
                     ELSDGPPRIVLAGHLDGERPVLVYRVADLDAAVAELEGRGWTPGHGLEIPQGPVRSFE
                     GPGGHRLAIYELTRPGVIETFAGRREF"
     gene            1439..2131
                     /locus_tag="AC162A_03005"
     CDS             1439..2131
                     /locus_tag="AC162A_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009834.1"
                     /translation="MTLIAVLVLVTAATTTGVWTLAVFSNTAQSSTTITAGRIFPGIR
                     TTSGFVVHDASGGGAEVDRSSAFAVVGDGRTVATTAWATAFAANRYLQFDMNAPLPAN
                     VGASAITFRLTVASASPSGTACGYVNVRRISDDGSVATYGSPASPLGCVTGTTPATLV
                     LALPAITTTDSANDLRIRVFGRDSAGAGSVIDGAMVTGDTPYSTYTLYPVRYTDAASS
                     TPVSVPWELDGP"
     gene            2214..>4302
                     /locus_tag="AC162A_03010"
     CDS             2214..>4302
                     /locus_tag="AC162A_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5009835.1"
                     /translation="MVVVALGSSIGVAPARALDRQPVAAGPVTTPTPPPTPEPTPTAR
                     PAPTPAPTATPTPEPVVTPAPTPGPSEAPATPAPSDLAPSAVPDPTPTPAPVDPVVEP
                     SAVPAASDVPATSPEPSPADVAMAPYLVTFVAEAGEATRADALTRAGATLVSEIPELR
                     LAVVELAAADAPDRADLLRADPAVARVERDRTRTTEGAPDDPRYPDQWSLPRIGWDDV
                     RDGPAPDGHAVVAILDTGVDASARDLASRLLPGVSFVDGVAPDTDPNGHGTWMAGIVG
                     ADTDNGTGIAGVAFGHVGVLPITVLGPDGTGYDSAIVAGIVAAVDAGADVILMSFSAR
                     GYSAVLQAAVDYAWAHDVVLVAATGNDDSTAPAYPAGDRGVIGVTSTNRADLLAPGSN
                     SGPTAFMAAPGVDILTTDGGGGYESVSGTSAAAAEVAGAAGLLRAFDPTASNGVIVGR
                     LARSAAPADTAERTGNGRLDLARAAADTDDTFVEPAGVAGTADGGPFVGPYVVAAVRT
                     WTGAGANNNWTTAANWGGTAPVAGDDLVFPAGAARLSNTNNYANGTVFNSITISGTGY
                     ALAGNRIALGATGLSSSAVGSANTLSLQLSFAATSTVTVTSATSTVTLSGLISGAGGL
                     TKAGAGTLALSVANSYTGPTAVNGGTLRLGIANGVGASSALSVASGATFDLNGFSDTV
                     GSVAGVAGSTITSG"
BASE COUNT          578 a         1617 c         1541 g          566 t
ORIGIN      
        1 cgtgatctgt accgcgttcc ggaaccccgc gctgacggcg aagatgatct cgacgatgga
       61 cgtcatcagc ggcggccgga tggagctcgg gatcggcgcc ggctggaagc gcgacgagtg
      121 ggatgcctac ggctacggct ttcccgagac gcgtgagcga ctcgcgcggc tggcggacga
      181 cctcgaggtc gtctcccgga tgatggacgg agaccggcac cagcacgcga ccttcgaggg
      241 cacctactcc agcgtcaccg acgcccgcaa cgtccccaag ccggtgcagc ggccgcacgt
      301 gccgatcatg gtcggcggca acggaccgaa tgtcacctgg cggctggccg cgaagttcgc
      361 cgacgagctg aacctcgacg ggatggaccc gtccgatgtc gccgccgccc tgccgacggt
      421 ccgggcgcgc tgcgaggaga tcgggcgcga ccccgcgacg ctggcgatct cggtgcacat
      481 gtgggcggag gacatcggcc tgcccgggca gtcgcgggtc gaccggctgg cgggcctgcg
      541 ggagctcggc gtcaaccggg cgatggggct cgtccgcgcg acgacgaccg acgacgaggc
      601 gctgccgagg ttcatcgagg acgcccgcgc cgccggcgtc gaggtcggct gaggcgggcc
      661 ctcgccgcta gaactcgcgc cggccggcga aggtctcgat cacgcccggc cgggtcagct
      721 cgtagatcgc gaggcggtgc ccgcccgggc cctcgaatga ccgcacggga ccctgtggaa
      781 tctcgaggcc gtgcccgggc gtccagccac ggccctcgag ctccgcgacc gccgcgtcga
      841 ggtcggcgac gcggtagacg agcacgggcc gctcgccatc gagatggccg gcgagcacga
      901 tccgcggcgg cccgtcgctc agctcgacca tcgccacccg cgtgcccatg gcctcgatcg
      961 cgaacacgag gcgtgccccc aggacatcgg tgaaataccg gacgtcggcc gcgacgtcgc
     1021 ggcttggcgt gtacaggtag tcgagcgact cgaacacctc gccatggtac gagcggccgg
     1081 gctgcgggcc tgctggtgcg ccgggatcgg cggacgcccc gtaccaatgg gtgatcggcg
     1141 atgtcccttc ggaggatgcc gcggttgggt gcggagtggt tggatggcct cgcgatcgcg
     1201 gagcagccgt ctattccccg tggtcgctgt cgcgcgcatg caccgggcgc atgcggcgcg
     1261 gcgagccgag tgcccgagtg catgcccgat caggaggcca tatggacgca ccgcggaccc
     1321 agaacccgtg cggtgccgcc gtctccagcc cacgccccgg agcgagctga ccttggcgag
     1381 ccactccgcc gcgcgtcgcg gcgcgctgcc ccgggtattc ggaccgattc gcctgcgggt
     1441 gaccctgatc gccgttctcg tgctcgtcac cgccgcgacg accaccggcg tgtggacgct
     1501 cgcggtgttc agcaatacgg cccagtcgtc gacgaccatc accgccggtc gaatcttccc
     1561 gggcatccgc acgacgtcgg ggttcgtcgt gcacgacgcg tcgggcggcg gggccgaggt
     1621 ggatcgctcg agcgcgttcg ccgtcgtcgg cgacggccga acggtcgcca ccacggcttg
     1681 ggccaccgcg ttcgcggcca accgctacct ccagttcgac atgaacgcgc cgctgcccgc
     1741 gaacgtcggg gcgtcggcga tcacgttccg cctcaccgtg gcgagtgcca gcccttcggg
     1801 caccgcgtgc ggctacgtga acgttcgccg catctccgac gacgggagcg tggcgacgta
     1861 cggaagcccg gcatcgcccc tcggctgtgt caccggaacg acgcccgcca cgctggtcct
     1921 cgcgctgccg gccatcacga ccaccgactc ggcgaacgat ctccggatcc gcgtgttcgg
     1981 ccgcgacagt gccggcgccg ggtcggtgat cgacggggcg atggtgacgg gcgacacgcc
     2041 ctactcgacg tacacgctct atccggtgcg ctacaccgac gcggcgagct cgacgccggt
     2101 gtccgtcccg tgggaactcg atggcccgta ggcccaggga cgctccgccg aagtcgaggc
     2161 ttccgcggtc gccgatctcc gcccaccgcg ccaggacggt cgccgtcctg gcgttggtcg
     2221 tcgtcgccct cggcagctcg atcggcgtgg cgccggcacg ggcgctcgat cgtcagccgg
     2281 tcgccgccgg tccggtcacg accccgacgc cgccgcccac gcctgagccg acgccgaccg
     2341 cgcgaccggc gccgacaccg gcccccacgg cgacgcccac gccggaaccg gtcgtcacgc
     2401 cggcgccgac ccccggtccc agcgaggcac cggcgacccc ggcacccagc gacctcgcgc
     2461 catccgccgt acccgatccc acgcccacgc cggctcccgt cgacccggtc gtcgagccca
     2521 gcgccgtccc ggcggcgtcc gatgtgccgg ccacatcccc ggagccctcg ccggccgacg
     2581 tcgcgatggc tccgtacctc gtcacgttcg tcgctgaggc cggcgaggcc acgcgagccg
     2641 atgcgcttac gcgcgcgggc gcgacgctcg tcagcgagat cccggagctg cgccttgcgg
     2701 tcgtcgagct ggccgcggcc gatgcccccg atcgagccga cctcctccgc gccgacccgg
     2761 ccgtcgcccg cgtcgagcgc gaccggacgc ggaccacgga gggtgcgcct gacgaccccc
     2821 gctaccccga ccagtggtcg ctgccacgga tcggatggga cgacgtccgc gatggccccg
     2881 cgcccgacgg tcatgccgtc gttgccatcc tggacacggg cgtcgacgcc tccgcgcgcg
     2941 acctcgccag ccggctgctg cccggggtct cgttcgttga cggtgtggcg cccgacacgg
     3001 accccaacgg ccacgggacg tggatggccg gcatcgtcgg cgccgacacc gacaacggca
     3061 ccggtattgc gggcgtcgcc ttcggccacg tcggggtgct gccgatcacg gtcctcggcc
     3121 cggacggcac ggggtacgac agcgcgatcg tcgcgggcat cgtggccgcg gtggacgcgg
     3181 gcgcggacgt gatcctgatg tcgttcagcg cgcgcggcta ttcggcggtc ctccaggcgg
     3241 cggtcgacta cgcgtgggcc catgacgttg tgctcgtggc cgcgacgggc aacgacgact
     3301 cgacggcccc ggcctatccc gccggcgatc gcggcgtgat cggcgtcacc tcgaccaacc
     3361 gggccgacct cctcgcgccg ggcagcaaca gcggacccac ggcgttcatg gccgcgcccg
     3421 gcgtcgacat cctcacgacg gacggcggcg gcggctacga gtccgtcagc ggcacctcgg
     3481 cggccgcggc ggaggtcgcg ggcgcagccg ggctgctgcg cgcattcgac cctaccgcct
     3541 cgaacggcgt catcgtcggg cgcctggccc ggagcgccgc tcctgccgac accgcggaac
     3601 gaaccggcaa cgggcgcctc gacctggccc gcgccgcggc ggacacggac gacacgttcg
     3661 tcgagccggc cggcgtcgcc gggaccgcag acggcgggcc gttcgtcggg ccctacgtgg
     3721 tcgcggctgt ccgaacgtgg acgggcgccg gagcgaacaa caactggacc acggcggcga
     3781 actggggcgg aacggcgccc gtcgccggcg acgacctcgt gttcccggcc ggtgcggcgc
     3841 ggctgtccaa cacgaacaac tacgccaacg ggacggtgtt caactcgatc acgatctccg
     3901 ggacgggcta tgcgctcgcg ggcaaccgga tcgccctcgg cgcgacgggc ctctcgtcga
     3961 gcgccgtcgg gtcggcgaac accctcagcc tgcagctgtc gtttgcggcg acgagcacgg
     4021 ttaccgtgac ctctgcgacc tcgacggtga cgctgtccgg cctcatcagc ggcgccggcg
     4081 gcctgaccaa ggccggggcg ggcacgctgg cgctctcggt cgccaactcg tacacggggc
     4141 cgacggcggt caacggcggc acgctccggc tgggtatcgc caacggggtg ggcgcgagct
     4201 cggcgctctc ggtcgcctcg ggcgccacgt tcgacctcaa cgggttctcg gacacggtcg
     4261 gctcggtcgc gggcgtggcc gggtccacga tcaccagcgg tg
//
LOCUS       DBNHXO010000147         2017 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1821458, whole genome shotgun sequence.
ACCESSION   DBNHXO010000147 DBNHXO010000000
VERSION     DBNHXO010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1821458"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="AC162A_03015"
     CDS             <1..443
                     /locus_tag="AC162A_03015"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR5009836.1"
                     /translation="IATDGPARARLWQIRERLPESLQREGVPHSIDAALPLPRLADFA
                     GLLPATIERASPGARLILFGHLLDGNLHVNVLGPDPDDPSVDDAVLGLALELGGTVSA
                     EHGIGVAKAAWLARDRSPGDLAAMRSIKRALDPDGILNPGVLLG"
     gene            complement(452..661)
                     /locus_tag="AC162A_03020"
     CDS             complement(452..661)
                     /locus_tag="AC162A_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009837.1"
                     /translation="MSEHRRRRAEPNAGAFLGPDILVPVPMGSAGARSEEPAEPEDDR
                     VPEPSPPGVLRRLRERLLRGRPAAS"
     gene            809..1828
                     /locus_tag="AC162A_03025"
     CDS             809..1828
                     /locus_tag="AC162A_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR5009838.1"
                     /translation="MPAVTVDDITVLPRIAVPDRIVARQRQVLQLTTAPSAFEGDGFP
                     VRRAFAGVLLEDLDPFIHLDQMGEVEYAPGEAKGTPWHPHRGFETVTYMLDGVFEHAD
                     SNGGGGLITNGDTQWMTAGAGILHIEKPPEWLVAQGGLFHGFQLWVNLPRDQKLAAPR
                     YQDIRASEVALASSPDGGALVRVIAGEVAGHAGPGSTYTPMTLVHATLSPGATLQLPW
                     RADYNALAYVLAGHGTIGVEGRPARLGQLAVFGPGDAMTFAADQLQESRAPNLDILIL
                     GGRPIREPVAWMGPFVMNTREEVLQAFADARSGRLGTIPAAYLAHTVHNAPATLQVTA
                     PADER"
BASE COUNT          279 a          774 c          691 g          273 t
ORIGIN      
        1 cgatcgcgac ggacggcccg gcccgcgccc ggctgtggca gatccgcgag cggctgccgg
       61 agtccctcca gcgcgagggc gtcccgcact cgatcgacgc cgcactcccg ctgccgcggc
      121 tggccgactt cgccggcctg ctgcccgcca ccatcgaacg ggcgtcgccc ggcgcccgcc
      181 tgatcctgtt cggccacctg ctcgacggca acctgcacgt caacgtcctc ggtcccgacc
      241 ccgacgaccc ctcggtcgac gacgctgtcc tcggcctcgc cctcgagctc gggggcacgg
      301 tcagcgcgga gcatgggatc ggcgtcgcca aggccgcctg gctggcccgc gaccgatcgc
      361 cgggcgacct cgccgcgatg cgctcgatca agcgggcgct cgaccccgac gggatcctga
      421 accccggcgt cctcctgggc taggtcggac ctcaggacgc cgccggccgt ccacgcagca
      481 gccgctcgcg caaccggcgg aggacgcccg gcggtgatgg ctcagggacg cgatcgtcct
      541 cgggttccgc cggctcttcg ctgcgggcgc cggcagatcc catcggcacg ggtaccagga
      601 tgtccgggcc gaggaacgct ccggcattgg gttcggcccg ccgacgtcga tgctcgctca
      661 tgaactgatc cctcgttcgc cctgcatgct gcgcgggttt cgcggtcgcg gcaagggccg
      721 aaggcccacc atcggccgat cgttgacatc gctgcgccga cctgagatag ttgcacgtgc
      781 aaccaactcg cacgaagaag cctgaccgat gcccgcagtc accgtcgacg acatcaccgt
      841 cctcccccgt atcgcggtcc ccgaccggat cgtcgcccgc cagcgtcagg tcctgcagct
      901 gaccacggcg cccagcgcgt tcgagggcga cggcttcccg gtccgccgcg cgttcgcggg
      961 cgtcctgctc gaggatctcg acccgttcat ccacctcgac cagatgggcg aggtcgagta
     1021 cgcaccgggc gaggccaagg gcacgccgtg gcatccccac cgcgggttcg agacggtgac
     1081 ctacatgctc gacggcgtgt tcgagcacgc cgactcgaac ggcggcggcg gcctgatcac
     1141 caacggcgac acgcaatgga tgaccgcggg cgccgggatc ctgcacatcg agaagccacc
     1201 ggaatggctg gtcgcccagg gcgggctgtt ccacggcttc cagctgtggg tcaacctgcc
     1261 gcgcgaccag aagctcgcgg cgccgcgcta ccaggacatc cgcgcgagcg aggtcgcgct
     1321 cgcctcatcg cccgacggcg gcgcgctcgt gcgcgtgatc gcgggcgagg tcgccggcca
     1381 cgccggaccc ggctcgacgt acacgccgat gaccctcgtc cacgcgacgc tcagcccggg
     1441 cgcgacgctc cagctgccct ggcgcgccga ctacaacgcg ctcgcgtacg tgctggccgg
     1501 acacggcacc atcggcgtcg agggacggcc ggcgaggctc ggccagctgg cggtcttcgg
     1561 gcctggcgac gcgatgacgt tcgccgcgga ccagctccag gagagccgcg cgcccaatct
     1621 cgacatcctg atcctcggcg ggcggccgat ccgcgagccc gtcgcctgga tggggccgtt
     1681 cgtgatgaac acgcgcgagg aggttctgca ggccttcgcc gacgcgcgat ccggccggct
     1741 cggcacgatc ccggcggcgt acctggccca caccgtgcat aacgccccgg cgacgctcca
     1801 ggtgaccgcg ccggccgacg agcgctagac cgcccttcgg gctaccagaa ccggcggcgc
     1861 gaggcgggct gcgcccgggg ggcgtggtag cgatccatca gctcggcgat cgcgtcacgc
     1921 gcggcgcgcc actcctcctg gtcgccgaac acgtcggggt cgcggcccca gcgctggatc
     1981 tgggtcatcg ccgtgacgat cgcgttgtgc tgggcca
//
LOCUS       DBNHXO010000148        11116 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4202476, whole genome shotgun sequence.
ACCESSION   DBNHXO010000148 DBNHXO010000000
VERSION     DBNHXO010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 11116)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11116)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11116
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4202476"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="AC162A_03030"
     CDS             <1..268
                     /locus_tag="AC162A_03030"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HIR5009839.1"
                     /translation="AQLAVAQASAEMYARELAKSGRGAITTEVREAPAFYFAEDYHQQ
                     YLAKNPNGYCPNHSTGVRFPDQAITPLQVRRVSPVSGEGAPADL"
     gene            276..731
                     /locus_tag="AC162A_03035"
     CDS             276..731
                     /locus_tag="AC162A_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007982180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5009840.1"
                     /translation="MTGPRMDNVLLVVDDLEAVKAFFADLGLELEGETVVEGESVDRL
                     IGLDGVRATIASMRTPDGHGRIELDKFHTPAAVRAEPEDAPVNALGIRRIMFAVDDIE
                     SVLGRLRRLGAELVGELVQYEDQYRLCYVRGPEGIIVALAEHSAEDVSN"
     gene            735..1331
                     /locus_tag="AC162A_03040"
     CDS             735..1331
                     /locus_tag="AC162A_03040"
                     /inference="COORDINATES: protein motif:HMM:NF017949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5009841.1"
                     /translation="MTDPIADIIGDYRAFAARQRDRLLARGIDIAPYALSHLAYRVPE
                     WDQYVQVRTLLERHATANRENVWNGRPISLIIPAKPLEVLEGQVVPLIELIPPVHQRV
                     YKMGLEHLGIVVGDKFDTFVGAHKPVLTGQQFQGPNSTPDPVYILFEDFTHVKFYRLS
                     LQASVELDGGRFEDGFHHVDDWVPQRLVTATGPNPLPR"
     gene            complement(1332..2264)
                     /locus_tag="AC162A_03045"
     CDS             complement(1332..2264)
                     /locus_tag="AC162A_03045"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR5009842.1"
                     /translation="MATRIILDVDTGTDDAVALMVAALSPAIELVGATAVNGNTGVDD
                     TTENTLRVFDLIGRPDIPVFRGMATPMVRAQGDRGMAAVIHRNLLDLPAARTQARPEH
                     AVDWLVDTYMDSQGDITLVAVGPLTNVAMALLKEPRMAEWIPEIVIMGGAHESGNRTA
                     SAEYNFWVDPEAARIVVECGRPIRMVPLDATHRAPVSLDDCARLRALGTVAADATAAI
                     VEQRIAGYNATQPMAGASTAPVHDALAVCAVIEPAVLTTRHIRVDVETTSTLAAGRTI
                     CDVRPTPDGIPNVEFAFDADGPRFVEMLVEILGR"
     gene            2389..3753
                     /locus_tag="AC162A_03050"
     CDS             2389..3753
                     /locus_tag="AC162A_03050"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015612855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HIR5009843.1"
                     /translation="MTPSPSVDTDPVRLAQAFPADFTWGFAASGHQIEGAAAEDGRGP
                     SIWDTFARQPGAIADGSNGDVACDHYHRYPEDVRLMADLGARVYRFSVSWSRVLPTGT
                     GAVNQRGLDFYERLVDSLLAAGVRPLVNLFHWDLPQALQDRGGFADPQVVGWFTDYAA
                     LLASKLGDRVADWMTFNEPACFAFLGHAEGIHAPGVRDWPTAIRVADNELRAHAGAAQ
                     AIHALVGGSKVGIAIDVNQVVPATDSERDRVAASRWSSARDAWFLDPLFGRGYPALGL
                     EAHREAGHLEGVELDEPPAGDLDYLGLNYYRRDSVAARSDRAFDWEIGAATDSEQTQM
                     DWHVAPDGLRDTLIELNETYAPPEIVVTENGAAYPDTVDQDGRVRDVDRVHYLARHVA
                     AAADALGAGVPLTGYHVWSLLDNYEWSLGFSRRFGLVHVDYASQRRTPKDSARWYQRL
                     IARE"
     gene            complement(3766..5322)
                     /locus_tag="AC162A_03055"
     CDS             complement(3766..5322)
                     /locus_tag="AC162A_03055"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR5009844.1"
                     /translation="MIAFAGTELPRTEARLITERGVAGVTLFRLHNVVDPPQIRRLTA
                     AIQAARPVGAPPLLVAADQEGGQLVGLGDGTTPFAGPMALGAAGDAALAERVAGAMAR
                     ELRAVGVNVNYAPTCDVASNPANPALGIRSFGDDPVAVGGLAAATVRGLQGEGVAATA
                     KHFPGAGDTASDPHHELPLVPRTTTELAERELVPFRAALDAGVRMVMTGHLRLPDLPR
                     DLPTSLSAAVLRDLLRGQLRFDGVTVTDALDMHALAQGSAQIVDAIAALRAGEDVLLG
                     TVDDAARERLAEGLAQAQRRGLIDADDDAAATRRLGELRRWLGEYDQPSIDVVGCRDH
                     QVLAAELARRSITLVRNDDGLLPLRPAADARIAVVQSMPADLTPADTSATVPPTLASA
                     LRRRHAGVEEILLPATLGAADITGLGERLAAVDLVVLGTFSAHLQPAQAALAAAVLAS
                     GTPTVTVALRTPWDLLAHPSARTHVCSYGILPPSMEALAAALLGEAPFAGRLPVDIAG
                     LHPRGHGLSA"
     gene            complement(5337..6233)
                     /locus_tag="AC162A_03060"
     CDS             complement(5337..6233)
                     /locus_tag="AC162A_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5009845.1"
                     /translation="MTAPSKPIAASKERTRFWRAPMLHSLPLRISVVVICLLWTLPTA
                     GLFISSFRNPQLITQTGWWTALIHPFEQGWTLQNYQQVIGSNGMGEAFLNSLIVTIPA
                     TVIPITIAAFAAYAFAWIPFRGRGILFIVVVALLVVPLQMALIPVLQIYSGLGLYGTF
                     IGVWLAHTAFGLPLAIFLLYNFMSQLPRDLFESASIDGASAFQTFIRIVLPLSIPALG
                     AFAIFQFLWVWNDLLVALVFLGPGADVRVMPTALFNLLGAYGGAWHLLTAGAFLTMIV
                     PLVVFLVLQRAFVRGILAGSVK"
     gene            complement(6233..7405)
                     /locus_tag="AC162A_03065"
     CDS             complement(6233..7405)
                     /locus_tag="AC162A_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006516105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5009846.1"
                     /translation="MAVDRAQGSGSYGTSRTVLVVSLIGLVLAAIGITVLIDKEGGQK
                     LLASIYDLLGNATGAEALRNGQGDQFFAKLLLAAIALLVGVGGIWLLFTGVSALVERL
                     RPALRDRILPLVFIAPAVVLLGVYLVYPAVRTVIGSFQDENGAFTLANWASLATPPFV
                     EILRNNILWLVIGTSGSVGLGLLTAALFDRIRRESLAKIFVFMPLAISLVGATVIWKF
                     VYAWQPPSQPQFGLLNAVWTALPFGFQPIPWATQFPINLPSEMVILIWLQTGFAMVVF
                     SAAIKGVSTEIIEAARLDGANERQLFFKVIIPMIRGTIVTVTTTIAIVTLKIFDIVMS
                     ISGGRNHDDVVAVRMFAEMFQFFNDGRAAALATVLFIAVLPVMVVNLRNFRRQAAM"
     gene            complement(7495..9009)
                     /locus_tag="AC162A_03070"
     CDS             complement(7495..9009)
                     /locus_tag="AC162A_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5009847.1"
                     /translation="MAKTLRTTAALSVVVMVLVGACSSSTATPSPAAATPAATSAPAT
                     PAASEPAASEPAASEPAASEAPALNLPAVPTGYTELDAALGADKPLNGKTVSIQTQWV
                     AGEGEGFANAIKDFQTATGIKVQVDSIGSSHETVLKTRVEGGQPPDLAAIAQPTAVLA
                     YAKDGKLQDVAALVGPAGATKLKDEFPSTIGLTSEGDKIWSIPTKADVKSMVWYPPKA
                     FAAKGYTVPKTWDELVALADKIVADGSVPFCASAGGPGTATGWQLTDWVEEVLLKTED
                     PQVTQDWISHKIPFTDPKIKAAFDRVGSLLFKQGYVDGGGQAIVNSDMRVVMDPMFNG
                     DTSTPGCWMQKIPVWYGPDFFPDRRVNGGDSKFTIGDDGDIGIFPFPTISEEFKGAEG
                     SADSFMVFNDRPEVRALAEFLATPEGLKGWIQSVGVLSPNVKVPADWYAANYKLKVAN
                     EIASGATAIGFDASDLMPPAVGQGTFWTGVSDWANNNGANTDAVLQAIDDSWPK"
     gene            complement(9105..10100)
                     /locus_tag="AC162A_03075"
     CDS             complement(9105..10100)
                     /locus_tag="AC162A_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR5009848.1"
                     /translation="MTYEPELLGPLVAGVDVGGTKTLVIVVDDEDRIVYEQAAPTNGS
                     SLVGQIAGLVDDARRRLGRDLTAVGVAIPGHVDADDGSVSMAVNLGITHVPLGAMLQA
                     QLGVPVFVEHDARAAALWLSNEADHWSAPSPASVAFLAIGTGISAGVVLNGALLRGDN
                     SFAGEVGHIVADPEGSVCACGLRGCLETIAAGPAIGRQADEAIAAGRSTVLLPLSSAA
                     DVFRASAAGDGVAVEIVDRVADHLARAIRSLALTLGVRRVVIGGGVAAAGPALLDPIR
                     SRIARERAMSPLVRAALGDAAVELLSPTEKPGARGAAAIARHRIGLPEREGVGER"
     gene            complement(10097..>11116)
                     /locus_tag="AC162A_03080"
     CDS             complement(10097..>11116)
                     /locus_tag="AC162A_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR5009849.1"
                     /translation="LVGTLIDDGLIEEVGRGQSSGGKSPILLRVAPDGRHLIGLDLGE
                     EQFSGAVVNLRGEILRSIHLPLDGRNGDGAVELVTELLDALRADDESPLLGIGIGAPG
                     VIDTSTGTVRWSVNLNWAELRLGPLLEQRYGVPVVVANDSHAAALAELTFFRRPRPNN
                     LIVIKVGRGVGAGIILNGQLFQGDGYGAGEFGHVSLGSRAAPCRCGREGCLETMASMR
                     ALVDAAGAVEPSITDESGLVAAFLAGVTGIRRIVLDAARELGIAIGWLIGVLNVRHVL
                     LVGPVAEFGDDWLGEVRRSARSSVLSLLGRDTQIEFGHVHDDVVVLGASALLMEQQLG
                     LGLVR"
BASE COUNT         1782 a         3817 c         3901 g         1616 t
ORIGIN      
        1 ggcccagctg gccgtggcgc aggcgtcagc cgagatgtac gcccgagagc tcgcgaagtc
       61 gggccggggc gcgatcacga ccgaggtccg cgaggcaccc gcgttctact tcgccgagga
      121 ctaccaccag cagtacctgg ccaagaaccc gaacggctac tgccccaacc actcgaccgg
      181 cgtccggttc ccggatcagg ccatcacccc gctccaggta cgtcgagtga gtccggtcag
      241 cggcgagggg gcgcccgccg acctatagtc gggcgatgac cggtccgcgg atggacaacg
      301 tgctcctcgt tgtcgacgac ctcgaggctg tcaaggcgtt cttcgccgac cttggcctgg
      361 agctcgaggg cgagacggtc gtcgagggcg agtcggtcga ccgcctcatc gggctcgacg
      421 gcgtccgagc caccatcgcc tcgatgcgga ccccggacgg ccacgggcgg atcgagctgg
      481 acaagttcca cacgccggcc gcggtcaggg ccgagccgga agacgcgccg gtgaacgcgc
      541 tgggcatccg gcgcatcatg ttcgcggtcg acgacatcga gagcgtcctt ggccgcctgc
      601 gccgcctcgg cgccgagctc gttggcgagc tggtgcagta cgaggaccaa tatcggctct
      661 gttacgttcg cggccccgag ggcatcatcg tcgccctggc agagcactca gctgaagacg
      721 tatcgaactg agagatgacc gacccgatcg cggacatcat cggcgactac cgcgcgttcg
      781 cggcccggca gcgagaccgg ttgctggcgc gtggcatcga catcgccccg tacgcgctca
      841 gccacctcgc ctaccgggtg cccgagtggg accagtacgt ccaggtccgg acgctcctcg
      901 agcgccacgc gaccgcgaat cgggagaacg tctggaacgg ccgcccgatc tccttgatca
      961 ttcccgccaa gccgctcgag gtcctggaag gccaagtcgt cccgctcatc gagctcatcc
     1021 cgcccgtcca ccagcgcgtc tacaagatgg ggctggagca cctcggcatc gtggtcggcg
     1081 acaagttcga cacgttcgtt ggcgcccaca agcccgtcct gacgggccag cagttccagg
     1141 gcccgaacag cacacctgac cccgtctaca tcctgttcga ggacttcacc cacgtgaagt
     1201 tctatcgact gtcactgcag gcatccgtcg agctcgatgg tggccgattc gaggacgggt
     1261 tccaccacgt cgacgactgg gtgcctcagc ggctggtcac ggccaccggc cccaacccgc
     1321 tgccgcgctg actaccgacc gaggatctcg acgagcatct ccacgaagcg cggcccgtca
     1381 gcgtcgaagg cgaactcgac gttcgggatc ccatcggggg tcgggcgcac atcgcagatc
     1441 gtgcgtcccg cagccagcgt cgacgtcgtc tcgacatcca cccggatgtg ccgggtcgtg
     1501 agcacggcag gttcgatgac ggcgcagacc gcgagggcat cgtgcacggg cgcggtcgat
     1561 gcgccggcca tcggctgggt cgcgttgtac ccagcgattc gctgctcgac gatcgccgcc
     1621 gtcgcgtccg ccgccacggt tccgagcgct cgaaggcgcg cgcagtcgtc gagagagacg
     1681 ggggcgcgat gggtcgcatc gagggggacc atccggatcg ggcggccaca ctcgacgacg
     1741 atccgggcgg cctcgggatc cacccagaag ttgtactcgg ccgatgccgt ccgattcccg
     1801 ctctcgtggg cgccgcccat gatgacgatc tccgggatcc actcggccat cctcggctcc
     1861 ttgaggaggg ccatcgccac gttcgtcagc ggcccgacgg caacgagcgt gatgtcgccc
     1921 tgggaatcca tgtacgtgtc gaccagccag tccaccgcgt gctccgggcg agcctgcgtc
     1981 cgggcagccg gcaggtccaa caggttgcga tggatgaccg cggccatccc ccggtccccc
     2041 tgggcccgca ccatcggtgt cgccatcccg cgaaagacgg ggatgtccgg ccgtccgatc
     2101 aggtcgaaca cacgaagcgt gttctccgtc gtgtcgtcga cgccggtgtt gccgttgacg
     2161 gcggtcgccc cgaccagctc gatggcgggc gacagcgcgg caaccatcag cgcgaccgca
     2221 tcgtccgtgc cggtatcgac gtccaggatg atcctcgtgg ccatgacgca agtctagggc
     2281 ggcgggacgg ctgccccgac caccggaatt ggccgcggcg actcaggcct cagccgacgc
     2341 gtccccgcgg gtctccggag ggtcgggcgc gccgtatacg atcgacgtgt gactccatcc
     2401 cccagcgtcg acaccgaccc agtccggctc gcccaagcct ttccggctga cttcacctgg
     2461 ggcttcgccg cctcgggcca ccagatcgag ggagcagcgg ccgaggacgg ccgaggaccg
     2521 tcgatctggg acacgtttgc ccgccagccg ggcgccatcg ccgatggcag caatggcgac
     2581 gtcgcctgcg accactacca ccgctacccc gaggacgtcc ggctgatggc cgatctgggc
     2641 gcacgcgtct atcggttcag cgtcagctgg tcgcgcgtcc tgcccacggg gacgggcgca
     2701 gtcaaccagc gcggcctcga cttctacgag cgcctcgtcg acagcctgct tgccgcgggc
     2761 gttcgaccgc tggtcaacct gttccactgg gacctgccgc aggcactcca ggaccgcggt
     2821 ggcttcgccg acccgcaggt cgtgggctgg ttcaccgact acgccgcgct actcgcgtcg
     2881 aagctcggcg accgcgtcgc cgactggatg acgttcaatg agccggcctg ctttgcgttt
     2941 ctcggccacg ccgaggggat ccatgcgccc ggtgtgcgcg actggccgac cgcgatccgc
     3001 gtcgccgaca acgagctgcg tgctcatgcc ggcgctgcgc aggcgatcca cgccctggtc
     3061 ggcggatcga aggtcggcat cgcgatcgac gtcaaccaag ttgtgccggc gaccgattcg
     3121 gagcgggatc gggtcgccgc cagccggtgg agctcggctc gggacgcgtg gttcctggat
     3181 ccgctgttcg ggcgtggcta cccggcgctg ggactcgagg cccaccgcga ggcgggtcac
     3241 ctcgaaggcg tcgagctcga cgagccgccg gccggcgatc tcgactacct gggcctgaac
     3301 tactaccggc gcgactcggt cgccgccagg tcggaccggg cgttcgactg ggagatcggc
     3361 gccgcaacgg actcggagca gacgcagatg gactggcacg tcgcgcccga cgggctacgc
     3421 gacaccctga tcgagctcaa cgagacgtac gcgccgcccg agatcgtggt caccgagaac
     3481 ggcgccgcgt accccgacac ggtcgatcag gacggccggg tgcgcgatgt ggaccgggtg
     3541 cattacctgg cacgccacgt cgccgctgcc gccgacgcgc tcggcgcggg cgtgcccctg
     3601 accggctacc acgtgtggtc gctgctcgac aactacgagt ggagccttgg cttcagccgg
     3661 cggttcggcc tggtccacgt cgactacgcc agccagcgcc gcacgcccaa ggacagcgcg
     3721 cgctggtatc agcggctcat cgcccgggag taggttcccc ggcggtcatg cgctgagccc
     3781 gtgaccgcgt ggatgcagac cggcgatgtc gaccggcagg cggccagcga agggcgcctc
     3841 gcccagcagc gcggccgcca gcgcctccat cgacggcggc aggatgccgt agctgcagac
     3901 gtgggtgcgg gccgacggat gggcgagcag gtcccacggc gtgcgcaggg cgacggtgac
     3961 ggtcggcgta ccggacgcga ggaccgccgc ggccagcgcc gcctgcgccg gctggaggtg
     4021 cgcggagaac gtccccagga cgacgaggtc gacggccgcg agccgttcgc cgagaccagt
     4081 gatgtcggcc gcgcctagcg tcgccggcag gaggatctcc tccaccccgg cgtgccggcg
     4141 gcggagcgcc gatgccagcg ttggcggcac cgtcgcggac gtgtcggcgg gcgtgaggtc
     4201 ggcgggcatc gactggacga ccgcgatccg cgcgtcggcg gccggccgca atggcagcag
     4261 cccgtcgtcg ttgcggacga gggtgatcga gcgtcgggcg agctcggcgg cgagaacctg
     4321 atggtcgcgg cacccgacga cgtcgatgga cggctggtcg tactcgccca gccatcgtcg
     4381 cagctcgccc aggcggcgcg tggccgcggc atcgtcatca gcatcgatca ggccgcgccg
     4441 ctgcgcctgc gcgaggccct ccgccagccg ttcgcgggcg gcgtcgtcaa cggtcccgag
     4501 cagcacgtcc tcgcctgcgc gaagggccgc gatggcgtcg acgatctgcg ccgatccctg
     4561 ggccagggcg tgcatgtcca gggcgtccgt gacggtcacg ccgtcgaacc gcagctgtcc
     4621 gcgcagcagg tcgcgcagga ccgccgctga caggctcgtt ggcaggtcgc ggggcaggtc
     4681 gggcagacgc aagtggccgg tcatgaccat ccgaacgccg gcatccaagg ccgctcgaaa
     4741 cgggaccagc tcccgctcgg ccagctcggt ggtcgtccgc ggcaccaggg ggagctcgtg
     4801 gtgcggatcg gacgccgtgt cgccggcacc agggaagtgc ttggcggtcg ccgcgacgcc
     4861 ctccccttgc aggccgcgga cggtcgcggc cgcaagccca cccacagcca cgggatcgtc
     4921 gccgaagctg cggatcccga gcgccgggtt ggccgggttg ctggccacgt cgcacgtcgg
     4981 cgcgtagttg acgttgacgc cgacggcacg cagctcgcgc gccatggcgc cggcgacgcg
     5041 ctcggccagg gccgcgtcgc ctgccgcgcc gagcgccatc ggccccgcga acggcgtcgt
     5101 cccgtcgccc aggccgacca gctggccgcc ctcctggtcg gcggcgacca gcagtggcgg
     5161 tgctccgacg gggcgtgccg cctggatggc ggccgtgagc ctgcggatct ggggcggatc
     5221 gacgacgttg tggaggcgga acagcgtcac gccggcaacg ccgcgctcgg tgatgagcct
     5281 ggcctccgtt cgcggcaact ccgtgccggc gaaggcgatc atgagccgcg ccagggtcac
     5341 ttgacagagc cggccaggat ccctcgcacg aatgctcgct gcagcaccag gaacacgacg
     5401 agcgggacga tcatcgtcag gaacgcgccc gcggtgagca ggtgccatgc gccgccgtag
     5461 gcaccgagca ggttgaacag cgccgtcggc atgacccgga cgtccgcccc aggcccgagg
     5521 aagacgaggg caacgagcag gtcgttccag acccacagga actggaagat cgcgaaggcg
     5581 ccgagcgccg ggatcgagag cggcagcacg atccggatga acgtctggaa cgcggacgcg
     5641 ccatcgatcg aggcggactc gaacaggtca cgcggcagtt gcgacatgaa gttgtagagc
     5701 aggaagatag cgagcggcag cccgaacgcc gtgtgggcca gccagacccc gatgaacgtg
     5761 ccgtacaggc cgaggccgct gtagatctga aggacgggga tcagtgccat ctggagcggc
     5821 acgacgagca gcgccacgac gacgatgaac aggatgcccc ggccgcggaa cgggatccag
     5881 gcgaaggcgt acgcggcgaa cgcggcgatc gtgatcggga tgaccgtggc cgggatggtc
     5941 acgatgaggc tgttcaagaa cgcctcaccc atgccgttcg acccgatgac ctgctggtag
     6001 ttctgcagcg tccatccctg ttcgaacggg tggatcaggg ccgtccacca gccggtctgg
     6061 gtgatcagct gggggttccg gaaggagctg atgaacagcc ccgccgtggg cagggtccac
     6121 aggaggcaga tgacgacgac ggagatgcgc agcgggaggc tgtggagcat cggcgcccgc
     6181 cagaacctgg tgcgctcctt ggatgcggcg atcggcttcg agggtgcagt catcacatcg
     6241 ccgcctgtcg tcggaagttg cgcaggttga cgaccattac cggcagcacg gcgatgaaca
     6301 ggacggtcgc cagggcagcg gcgcgcccat cgttgaagaa ctggaacatc tccgcgaaca
     6361 tccgcaccgc gaccacgtcg tcgtggttgc gtcccccgct gatcgacatg acgatgtcga
     6421 agatcttgag ggtgacgatc gcgatcgtcg tggtcactgt cacgatcgtg ccccggatca
     6481 tcggaatgat caccttgaag aacagctggc gctcgttggc gccgtcgagc cttgccgcct
     6541 cgatgatctc cgtcgacacg cccttgatgg ccgccgagaa cacgaccatg gcgaagcccg
     6601 tctggagcca gatcaggatc accatctccg acgggaggtt gatgggaaac tgtgtggccc
     6661 atgggatcgg ctggaagccg aacggcaatg ccgtccagac ggcgttcagg agcccgaact
     6721 gcggctggct cggcggctgc caggcgtaga cgaacttcca gatgaccgtt gcgcccacca
     6781 gcgagatcgc gagcggcatg aagacgaaga ttttcgccag ggactcgcgc cggatgcggt
     6841 cgaagagggc ggccgtgagc aggccgagcc cgacgctgcc ggacgtcccg atcacgagcc
     6901 acaggatgtt gttgcgcagg atctcgacga atgggggcgt agccagactc gcccagttgg
     6961 cgagcgtgaa cgcgccgttc tcgtcctgga agctgccgat gaccgtgcgg accgccggat
     7021 acacgaggta gaccccgagc agcaccacgg ccggtgcgat gaagaccagg ggaaggattc
     7081 ggtcacgaag cgctggtcgc aggcgctcga cgagcgcgct cacgcccgtg aacaggagcc
     7141 agatcccgcc cacgccgacc agcagcgcga tggccgcgag caggagcttc gcgaagaact
     7201 gatcgccctg gccgttccga agcgcctcgg cgcccgtggc attgccgagc aggtcgtaga
     7261 tcgaggcgag gagcttctga ccgccctcct tgtcgatcag gacggtgatg ccgatggccg
     7321 cgaggaccag cccgatcagg ctgacgacca gcaccgtgcg cgacgtgccg tacgagccgg
     7381 atccctgcgc gcggtcgacc gccaccgttg ctcctccgag gacgttgccg gaatagtagc
     7441 ggggcgccca ccggttggtg tggcgccccg cgcagttacg ttgcgatgtg ccggctactt
     7501 gggccagctg tcgtcgatgg cctggagcac ggcgtccgtg ttggcgccgt tgttgttggc
     7561 ccagtccgag acgccggtcc agaaggtgcc ctggccgacg gccggaggca tcaggtcgga
     7621 ggcgtcgaaa ccgatggcgg ttgcgccgct ggcgatctcg ttcgcgacct tcagcttgta
     7681 gttggcggca taccagtcgg ccggcacctt gacgttcggc gacaggacgc cgacgctctg
     7741 gatccagccc ttgaggccct cgggcgtcgc caggaactcg gcgagcgcgc gaacctcggg
     7801 tcggtcgttg aacaccatga acgagtcggc cgaaccctcg gcacccttga actcttcgct
     7861 gatggtcggg aacgggaaga tcccgatgtc cccgtcgtcg ccgatggtga acttcgagtc
     7921 accaccgttg acgcggcgat ccgggaagaa gtccgggccg taccagacgg ggatcttctg
     7981 catccagcaa ccgggcgtgg acgtgtcacc gttgaacatc gggtccatga cgacccgcat
     8041 gtcggagttc acgatcgcct ggccaccgcc gtcgacgtag ccctgcttga acaggagcga
     8101 gccgacccga tcgaaggcag ccttgatctt ggggtcggtg aacgggatct tgtggctgat
     8161 ccagtcctgg gtgacctgcg gatcctcggt cttgagaaga acctcctcga cccagtccgt
     8221 cagctgccag ccggtcgccg tgccggggcc gccggcgctc gcgcagaacg gcacgctgcc
     8281 gtcggcgacg atcttgtcgg ccaaggcgac gagctcgtcc caggtcttgg gcaccgtgta
     8341 gcccttggcc gcgaaagcct tcggcgggta ccagaccatc gacttgacgt cggccttggt
     8401 cgggatgctc cagatcttgt cgccctcgct ggtgaggccg atggtcgacg ggaattcgtc
     8461 cttgagcttc gtggcgcccg cggggcccac gagcgcggcg acgtcctgaa gcttgccgtc
     8521 cttcgcgtac gcgagcaccg cagtcggctg ggcgatggcc gccaagtcgg gcggctgccc
     8581 accctcgacc cgggtcttaa gcacggtctc gtggctcgag ccgatgctgt cgacctggac
     8641 cttgatgccg gtcgccgtct ggaagtcctt gatggcgttg gcgaagcctt cgccctcacc
     8701 ggctacccac tgcgtctgga tcgagaccgt cttgccgttg aggggcttgt cggcgccgag
     8761 ggccgcgtcc agctcggtgt agccggtcgg gaccgccggc aggttcaggg ccggcgcctc
     8821 ggacgcggcg ggctcggacg cggcgggctc ggacgcggcg ggctcggacg cggcgggtgt
     8881 cgccggtgcc gacgtcgccg ccggcgtggc tgccgctggt gacggcgtcg cggtgctact
     8941 cgagcacgct ccgaccagca ccatgacgac gaccgacaag gccgccgtcg tgcgtagtgt
     9001 cttggccatg aattgactcc tcctgcgaag taccacgact gtggggacga gtggctccgc
     9061 tggaactact atcccattcc agcgcaaaag ccgtcgtgct ttcgttatcg ctcgcccacc
     9121 ccctctcgct caggcaagcc gatgcggtgg cgcgcgatgg cggctgcccc gcgcgcgccg
     9181 ggcttttcgg ttggggacaa cagttcgacg gccgcatcgc ccagggcggc ccggacgagc
     9241 ggcgacatcg cgcgttcgcg ggcgatgcgc gatcggattg gatcgaggag ggccggtccg
     9301 gcggccgcga cgccgccgcc gatgacgacc cgcctgacac cgagggtcag ggcgagcgat
     9361 cggatcgccc gggccaaatg gtcggccaca cggtcgacga tctcgaccgc gaccccgtcg
     9421 ccggcagcac tggcgcggaa gacgtccgcg gcactcgaga ggggcaacag gacggtgctg
     9481 cgcccggcgg cgatcgcctc gtcggcctga cgaccgatcg ccggaccggc ggcgatcgtc
     9541 tcgagacagc cgcgcagacc gcaggcacaa acggatccct ccgggtcggc gacgatgtgg
     9601 cccacctcac cggcgaagct gttgtcgccg cggagcagtg caccgttgag gacgacaccg
     9661 gccgagatgc cggtcccgat cgcgaggaag gcgacgctcg ccggcgaggg cgccgaccag
     9721 tggtccgcct cgttgctgag ccagagcgcc gcggcgcggg cgtcgtgctc gacgaagaca
     9781 ggcacgccga gctgcgcctg cagcatcgcc cccagcggca cgtgggtgat ccccaggttg
     9841 acggccatgc tgaccgagcc gtcgtccgca tcgacgtggc cggggatggc gacaccgacc
     9901 gccgtgaggt cacggcccag ccggcgcctg gcgtcgtcga cgagtccggc gatctggccg
     9961 accagcgatg atccgttcgt cggcgcggcc tgctcgtaga cgatgcggtc ctcatcgtcg
    10021 acgacgatga ccaacgtctt ggtgccacca acatcgacgc cggcgaccag cggcccgagg
    10081 agctccggct cgtaggtcat cgcaccaacc ccaggccgag ctgctgttcc atgagcagcg
    10141 ccgacgcgcc cagcacgacg acgtcgtcgt ggacgtggcc gaactcgatc tgcgtgtcgc
    10201 gtccgagcag tgacaggacg ctcgaccggg cggaccgccg gacctcgccc agccagtcgt
    10261 cgccgaactc cgcgaccggg ccgaccagca gcacgtggcg gacgttgagc accccgatca
    10321 gccagccaat cgcgataccg agctcgcgcg cggcatcgag gacgatccgc cggatgccgg
    10381 tgacgccggc caggaaggcg gccacgaggc cgctctcgtc ggtgatcgag ggctcgacgg
    10441 cgccggcggc gtcgaccaac gcgcgcatgc tggccatcgt ctcgaggcaa ccctcccgcc
    10501 cgcagcggca cggagcagct ctgctgccca gcgagacgtg gccgaactcg ccggcgccat
    10561 agccgtcgcc ctggaacaac tggccgttga ggatgatccc ggcgccgacc cctcgcccca
    10621 ccttgatcac gatcaggttg ttgggccgag gccggcgaaa gaacgtcagc tcggcgaggg
    10681 cggcagcgtg actgtcgttc gccaccacga ccggcacgcc gtagcgctgc tcgagcagcg
    10741 gcccgaggcg gagctcggcc cagttcaggt tgaccgacca gcggacggtc ccggtgctcg
    10801 tgtcgatgac gccgggcgcg ccgatgccga tgccgaggag gggcgactcg tcatcggcgc
    10861 gcagggcgtc gagaagttca gtgacgagct cgacggcgcc gtcgccgttc cgtccgtcga
    10921 ggggcaggtg gatcgagcgg aggatctcgc cacgcaggtt gacgacggca ccgctgaact
    10981 gctcctcgcc gagatcgagg ccgatcaggt ggcgaccgtc gggagccacc cgcaggagga
    11041 tcggtgactt gccgccgctc gactggccac gaccgacctc ttcgatcagg ccgtcgtcga
    11101 tgagcgtgcc gacgag
//
LOCUS       DBNHXO010000149         5599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4996802, whole genome shotgun sequence.
ACCESSION   DBNHXO010000149 DBNHXO010000000
VERSION     DBNHXO010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4996802"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1691)
                     /locus_tag="AC162A_03085"
     CDS             complement(<1..1691)
                     /locus_tag="AC162A_03085"
                     /inference="COORDINATES: protein motif:HMM:NF018332.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HIR5009850.1"
                     /translation="MESLPATYEVPSLEEDPALGLSHRAKMEILFAVMLGLFLGALDQ
                     TIVGPALPTIVTQLSGNDYYVWAITIYLLTSTISVPFWGKLSDVYGRKPIFMIGIVIF
                     LIGSALSGLSQNMAMLILFRGIQGIGAGSLFPVALAVIGDLFTPQERGKYQGLFGAVF
                     GIAFVAGPLIGGFLTESVGWHWIFYVNIPIGIIALIVIQRLLPTVKTKSATRNFDLIG
                     GFIFTVAMVFLLVGLTNKQFSDWSDFSVGGFIAIGLVGTLLFIFAESRAKEPIIPLDL
                     WKGRTYSATMVSVFFAAFAFFGAIVFLPRWFQVVEGFSPTNSGLAALPLMIGLIFSSI
                     VSGLIVSRTGHYKWLIVGSTILMGFAVLLMTSLTKDTSVPMVWLWMLIAGLGVGPTFS
                     VPTIVIQNAVPFRQLGVATSNLTFFRQIGGTIALAFVGTIFGTTFKDQLVPQMQAAGV
                     PAPVLAGFGQASAGGGFDFNQLTGVGDMGAAILASVPPAFQAIVAPAVPAMVEGIHGA
                     FSLAVGQTFWLGVFGAIVATIAAVAIKEIPLRTSNEQPVYGEAPAAAGLAPKPVA"
     gene            complement(1712..2332)
                     /locus_tag="AC162A_03090"
     CDS             complement(1712..2332)
                     /locus_tag="AC162A_03090"
                     /inference="COORDINATES: protein motif:HMM:NF047172.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR5009851.1"
                     /translation="MSTIIHVTQTIDAAATTRGRRDSTSDQLTDSIIADFRVTIGAMK
                     CAMSERLVRLGISMAQLNIMYTLQRSGEMPMSRLAELLNVSLSNATGLIDRMEERGFI
                     ERTRVPEDRRVVLVHVTEAGTRMIQENDALSDELMRDVLARLDRAELPVIARAVAEVR
                     AALEATAAQSAPDWRPISTQNPRSRSTIHNATPRSARSRPFAPTTP"
     gene            2383..3342
                     /locus_tag="AC162A_03095"
     CDS             2383..3342
                     /locus_tag="AC162A_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR5009852.1"
                     /translation="MRFALMIEPQQGLSYDEQLAVALRAEAAGFETLFRSDHYESFPG
                     GSGRPTTDAWAVLAGLARDTERIGLGALVSPVTYRNPGNLAKVAATIDEMSGGRIEIG
                     VGAGWHAAEHRRHGFPFPEIGERAEMLEETLEILRGLWDEPDGWSFIGKHYAIEDSLF
                     QPKPSALPARNGGRPPIIVGGEGSPRSFRIAARYADEFNLSSASPAVAVQKFAALDET
                     LKAAGREPSSIVHSAMVGLLMGRDADEVKARESDLLTSFGESEGAEAWFAPRRARWIH
                     GTPDQARKALGRFDDAGVERIMLQDFLPRDLEMIDLAAESLFG"
     gene            complement(3579..5420)
                     /locus_tag="AC162A_03100"
     CDS             complement(3579..5420)
                     /locus_tag="AC162A_03100"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5009853.1"
                     /translation="MTQQFARVPADAGDVVRQVRGFGGVPWSRLFRYLRPQLPIFSVA
                     IVGLLMSSVIGLLFPLIIAGITGEVVGGGDSADLDRLIALLIGLFCIQAVGSFIQTWL
                     LGVVGERVVAQLRGELYGRLVTLSLDFHGQHRVGELVSRLSSDVTLVRTLLTQTVTSL
                     LSSVIGLVGSVIILFTLSPSLLLVVLLLAPALIAVAIVFGRPLQRVSTQVQDTIASST
                     TTAEEVLSGIRVVKSYVREDWELKRYDVDLQGVVTAGSRLALWRAGFGALMGFLGFGA
                     IAALLWYTGHQVIEGSLGIGTLTGFLLYGVAIGASLGSIAGLYGQFREGTGAVTRVFE
                     IIDTRPTVVDAPDAVAIGPTEGRITLNGVTFAYEAGHPVLRSIDLDVGAGESLALVGP
                     SGSGKTTLVGLIPRLWDVSAGSIAIDGQDVRNVTTASLRERIGLVAQEATLFGGTIRD
                     NIRYGRLDATQDEIEAAASDANAHAFIAALSDGYDTIVGDRGMRLSGGQRQRVAIARA
                     ILKDPPILLLDEATSSLDNESERLVQEALDRLKVGRTTIIVAHRLSTIRAADRIAVLD
                     DGWLVELGTQDELLAHDGLYARLHRLQFTEPDELDESSTNPLEVVVG"
BASE COUNT          950 a         1816 c         1998 g          835 t
ORIGIN      
        1 tccgcgaccg gcttgggggc caggccggcg gccgccggag cttcgccgta caccggctgc
       61 tcgttggagg tccgcagcgg gatctccttg atcgcgacgg ccgcgatggt cgcgacgatc
      121 gcaccgaaca cgccgagcca gaaggtctgc ccgacggcga ggctgaaggc gccgtggatg
      181 ccctcgacca tcgcggggac ggcgggggcg acgatcgcct ggaacgccgg cgggaccgag
      241 gcgaggatgg ccgcgcccat gtcgcccacg cccgtcagct ggttgaagtc gaacccgcca
      301 ccggcgctcg cctggccgaa gcccgcgagg acgggtgccg gcacgccggc cgcctgcatc
      361 tggggcacga gctggtcctt gaacgtcgtc ccgaagatgg tgccgacgaa ggccagggcg
      421 atcgtgccgc cgatctggcg gaagaaggtg aggttcgatg tcgcgacgcc gagctggcgg
      481 aacggcaccg cgttctggat gacgatcgtc ggcaccgaga acgtcgggcc gacgcccagg
      541 ccggcgatga gcatccacag ccagaccatc ggcacggacg tgtccttggt caggctggtc
      601 atcaggagga cggcgaatcc catcaaaatc gtcgagccca cgatcagcca cttgtagtgg
      661 cccgtcctgg acacgatgag gccggacacg atcgagctga agatcaggcc gatcatcagc
      721 ggcagggccg ccaggccgga gttggtgggg gagaagccct cgaccacctg gaaccagcgc
      781 ggcaggaaca cgatcgcgcc gaagaacgcg aaggcggcga agaagaccga gaccatggtg
      841 gccgagtagg tgcggccctt ccacaggtcg agcgggatga tcggttcctt ggcgcgcgac
      901 tcggcaaaga tgaacagcag ggtcccgacg aggccgatgg cgatgaaccc gccgaccgag
      961 aagtccgacc agtcgctgaa ctgcttgttg gtcaggccga ccagcaggaa caccatggcg
     1021 acggtgaaga tgaacccgcc gatcaggtcg aagttgcggg tcgccgactt ggtcttgacc
     1081 gtcggcagga gccgctggat gacgatcagc gcgatgatcc cgatcgggat gttgacgtag
     1141 aagatccagt gccagccgac actctcggtc aggaagccgc cgatgagcgg gccggcgacg
     1201 aacgcgatgc cgaacacggc accgaacagg ccctggtact tgccgcgttc ctggggcgtg
     1261 aacaggtcac cgatgaccgc cagcgcgacc gggaacagcg atccggcgcc gatgccctgg
     1321 atgcctcgga agaggatgag catcgccatg ttctggctga ggcccgacaa cgcggatccg
     1381 atcaggaaga tcacgatgcc gatcatgaag atcggcttgc ggccgtagac gtcggacagc
     1441 ttgccccaga acgggacgct gatcgtggac gtgagcaggt agatcgtgat cgcccagacg
     1501 tagtagtcgt tgcccgacag ctgggtcacg atggtgggga gcgccggacc gacgatcgtc
     1561 tggtccaggg cgccgaggaa caggccgagc atgacggcga agaggatctc catcttcgcc
     1621 cggtggctca gcccgagcgc cggatcttct tccagcgacg gcacttcgta cgtggcaggg
     1681 aggctttcca tgggttccgt tcatccctcc ttcagggcgt ggtcggggcg aacggccggg
     1741 agcgggccga acgcggggtc gcgttgtgga tcgtcgagcg cgatcgtggg ttttgtgtgg
     1801 agatcgggcg ccaatccggt gcagattggg ccgcggtcgc ctcgagcgcc gcgcggacct
     1861 cggcgacggc acgggcgatg accgggagct ccgcccggtc caggcgggcc aggacatcgc
     1921 gcatcagctc gtcgctgagg gcgtcgttct cctggatcat ccgcgtgccg gcttcggtca
     1981 cgtggacgag cacgacccgc cggtcctcgg ggacacgggt gcgttcgatg aacccgcgct
     2041 cctccatccg gtcgatcagg cccgtcgcgt tggacaggga cacgttcaac agctccgcga
     2101 gacggctcat cggcatctcg ccgctgcgct gcaacgtgta catgatgttg agctgggcca
     2161 tgctgatgcc caggcgcacg aggcgttcgc tcatcgcgca cttcatcgcg ccaatcgtga
     2221 cgcggaagtc ggcgatgatg ctgtcggtca gctgatcgct cgtggagtcg cggcgtccgc
     2281 gggttgttgc ggccgcatca atagtttgtg tcacgtgaat gatggtactc atgtaaatca
     2341 tcctgtcaac atggatgatt tgcccaacgt gcaggagtcg agatgcgctt cgcgctcatg
     2401 atcgagcccc agcagggtct cagctacgac gagcagctcg ccgtcgccct ccgcgccgag
     2461 gcggcaggct tcgagaccct gttccgttct gaccactacg agagctttcc gggcggttct
     2521 ggccgaccca caacagatgc gtgggcggtc ctggcgggtc tggcgcgcga cacggagcgg
     2581 atcggcctcg gtgcgctggt gtcgccggtg acctaccgaa accccggaaa cctcgccaag
     2641 gtcgcggcga cgatcgacga gatgagcggc gggcggatcg agatcggcgt cggcgccgga
     2701 tggcacgcgg cggagcatcg gcggcatggg ttcccgttcc cggagatcgg tgagcgggcc
     2761 gagatgctcg aggagacgct ggagatcctg cgtggtttgt gggacgagcc ggacggctgg
     2821 tcgttcatcg gcaagcacta cgcgatcgag gactcgctgt tccagcccaa gccgagcgcc
     2881 ctcccggccc ggaacggcgg gcggccgccg atcatcgtcg gcggcgaggg gtcaccgcgc
     2941 tcgttccgga tcgccgcccg gtacgccgac gagttcaacc tctcatcggc gagccccgcg
     3001 gtcgcggtcc agaagttcgc ggcgctcgac gagaccctga aggccgcggg acgcgagccg
     3061 tcgtcgatcg tgcactcggc gatggtcggc ctgctcatgg gtcgcgacgc cgacgaggtg
     3121 aaggctcgcg agtcggacct gctgacgtcg ttcggcgagt ccgagggcgc cgaggcgtgg
     3181 ttcgcgccgc gtcgcgcgcg ctggatccac ggcacgcccg atcaggcgcg gaaggcgctc
     3241 gggcgattcg acgacgccgg ggtcgagcgg atcatgctcc aggatttcct gccccgcgat
     3301 ctcgagatga tcgacctcgc cgcggagtcg ctgttcggct gatcgcccgg cgtctcgctg
     3361 tcgcccgccg tatgcgtacc agggttacca ggcacgcgtg caagaacccg aggggccgct
     3421 atccactcgc gcagatgtgt ggcgaatatt ccgggccaca tcggggcgtg gagagcctcc
     3481 gcgaagatcg caacgggtcg gcaacccgtc cggtaaacct gtcacgtata cggcgcaggc
     3541 cggcgggcgg gtagcttgct gacggtcgcg gccaaggatc agccgacgac cacctcgagc
     3601 gggttggtcg aggactcgtc gagctcatcg ggctcggtga actggagtcg atgcaggcgg
     3661 gcgtacaggc cgtcgtgcgc gagcagctcg tcctgcgtcc cgagctccac gagccagccg
     3721 tcgtcgagca cggcgatgcg gtcggcggcg cggatcgtcg agagtcgatg ggccacgatg
     3781 atcgtcgtcc ggccgacctt gagtcgatcg agcgcctcct ggacgagccg ctccgactcg
     3841 ttgtcgagcg agcttgtcgc ctcgtcgagc agcaggatcg gagggtcctt gaggatcgcc
     3901 cgtgcgatcg cgacgcgctg gcgctggccg cccgagagcc gcatcccgcg atcgccgacg
     3961 atggtgtcgt acccgtcgga gagcgccgcg atgaacgcat gggcattggc gtcgctggcg
     4021 gcggcctcga tctcgtcctg ggtcgcgtcg aggcggccgt accggatgtt gtcgcggatg
     4081 gtgccgccga agagcgtcgc ttcctgggcc acgaggccga tccgctcgcg caggctcgcc
     4141 gtcgtgacgt tccggacgtc ctgaccatcg atggcgatgc tgcccgcgga cacgtcccac
     4201 agacgtggga tcaggccgac gagcgtggtc ttgcccgatc cggacggccc caccagggca
     4261 aggctctccc ctgccccgac gtcgaggtcg atggatcgca ggaccgggtg acccgcctcg
     4321 taggcgaagg tcacaccgtt gagcgtgatg cggccctcgg tcgggccgat cgcgacggca
     4381 tcgggcgcat cgaccacggt cgggcgggtg tcgatgatct cgaacacccg ggtcaccgcg
     4441 cccgtcccct cgcgaaactg gccatatagc ccggcgattg agccgaggct cgcgccgatg
     4501 gccacgccgt agagcaggaa cccggtcagg gtgccgatcc cgagcgatcc ctcgatgacc
     4561 tggtgaccgg tgtaccagag cagggccgcg atggcaccga acccgaggaa gcccatgagc
     4621 gccccgaagc ccgcccgcca cagggccagg cgcgatcctg cggtaacgac cccctgcaga
     4681 tcgacgtcgt agcgcttgag ctcccagtcc tcgcgcacgt agctcttgac cacgcggatc
     4741 ccggacagga cctcctctgc cgtggtcgta ctgctcgcga tggtgtcctg gacctgcgtg
     4801 ctgacccgct gcagcggacg cccgaacacg atggcgaccg cgatgagcgc cggcgccagc
     4861 agcagcacca cgagcaggag cgacgggctg agggtgaaca ggatgatgac cgacccgacg
     4921 agcccgatga ccgacgagag caggctcgtg acggtctggg tgagcagggt ccgcacgagg
     4981 gtgacgtcgc tcgagaggcg ggacacgagc tcgccgacgc gatgctgccc gtggaagtcg
     5041 agcgatagcg tcaccagccg gccgtacagc tctccgcgga gctgggcgac gacccgctcg
     5101 ccgacgacgc cgagcagcca ggtctggatg aagctgccca cggcctggat gcagaacagc
     5161 ccgatcagga gtgcgatgag ccggtcgagg tcggcagagt cgccgccgcc gaccacctcg
     5221 ccggtgatcc cggcgatgat cagtgggaac aggagcccga tgaccgagct catcagcagg
     5281 ccgacgattg cgaccgagaa gatcgggagc tgcggccgca ggtagcggaa gagccgcgac
     5341 catggcacgc ctccgaagcc gcgcacctgg cggacgacgt cgccggcatc ggcgggcacg
     5401 cgcgcgaact gctgggtcat gccaccgatc atggcacggg tcggctgtcg gtccatggaa
     5461 gcgctgtccg gaggagcacg ctcgctcgct cgatgctcgc gcagcgagcc ctgcagcgcg
     5521 gttcgccgac ccgcagcact gagcccgggc atcggtcgag cgtcaccgcc atacgccaca
     5581 ggccggcggg gtccctgct
//
LOCUS       DBNHXO010000150         3555 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_829851, whole genome shotgun sequence.
ACCESSION   DBNHXO010000150 DBNHXO010000000
VERSION     DBNHXO010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_829851"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="AC162A_03105"
     CDS             complement(<1..517)
                     /locus_tag="AC162A_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009854.1"
                     /translation="MSSSRSPRPEPGPSQPRPRPSEERAQLAQASQTTRRIAWIAVVV
                     ALVALGLAAWRAIVPAGSSCQDAAWNATPRDRDLPAGWTVSASQYDVDRKQMTIVGNE
                     PTDEATAQAVVYATITCFASGADDAVTRSADAAEAAGQIVTDRDDLGDQAFSADDGSG
                     ATFLQLRHGDVV"
     gene            complement(564..968)
                     /locus_tag="AC162A_03110"
     CDS             complement(564..968)
                     /locus_tag="AC162A_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6855 family protein"
                     /protein_id="HIR5009855.1"
                     /translation="MAGAGTREDPWQLVTANGGSQYQMWRDEAADPPALLCQVGGTQL
                     RYDLRAIDDLHAWLKAQGDWVPLGGADEQKPAADGTVEAFGRSDANPVGGWYGLKKGL
                     RGRFGVYLPPLLEALGLAELEHNPKNNRMRAR"
     gene            complement(1017..1799)
                     /locus_tag="AC162A_03115"
     CDS             complement(1017..1799)
                     /locus_tag="AC162A_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009856.1"
                     /translation="MSAAKEVDVEGALRMAAASANWPATPDLSSRVLARIEGGAEGAR
                     TAPTARRTRLRLVSALALALVTLLVLAGIAGALGFRLPGIEIVVVESLPPPSSGPPAG
                     SGLDLGSPVPLAEARAIDEPRVLLPAGLPEPDVAYVLGAGDRRIVTLAWRAEASQPTI
                     PSSDLALSLMAVPGGTNDALLKKLVARSSSIESVSVGGDRGWWISGAPHEIFVTRPDG
                     DVDMVMSALAGDTLVFARDGTLYRLESALGRDATIAIAEGLR"
     gene            complement(1796..2398)
                     /locus_tag="AC162A_03120"
     CDS             complement(1796..2398)
                     /locus_tag="AC162A_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5009857.1"
                     /translation="MQPRAPDEPGAIETALVRRAQAGDTAAYGELVTIHEAAAFRVAY
                     LLLGSASDAEDAAQEGFVRAYLALARFRAGEPFRPWLLQVVGNEARNRRRALGRRAGM
                     LDRAVRAFRGDDGSSVAPSPERTLLAGETNAEVREALGRLREEERQVVACRYLLELSE
                     AETAAVLGIPAGTVKSRLHRALARLRDDLAQIGALAEASR"
     gene            complement(2406..3089)
                     /locus_tag="AC162A_03125"
     CDS             complement(2406..3089)
                     /locus_tag="AC162A_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009858.1"
                     /translation="MRNPSHRPHRILAIAGALVLGATALAAPVLAKEGMAAELDAPVA
                     GGTPGGTTLLIGMTVTVLYDGTQHLVDGSTLYVGLIGPDGAATRAMAQEGARGHYTAR
                     VEVPASGVAGIEVGFDGSTDRPLMITGTAIVPGAITARTAQVAPVVTPITPIARASAG
                     PPVVAKPAVAPPAVAPTAQPAATPSPAPMVLAAGLVALVLLIAAAVIASRRSRLVAGL
                     PVSDPVREA"
     gene            complement(3120..>3555)
                     /locus_tag="AC162A_03130"
     CDS             complement(3120..>3555)
                     /locus_tag="AC162A_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018841515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR5009859.1"
                     /translation="CDEISLGDTIGAATPGDVNRVLDAELPIVDVARVGLHAHDTRGQ
                     ALANVFAALERGVTIVDSSAGGLGGCPFAGPGAVGNLATEDLVYLLDGLGIEHGVSLE
                     GVMAASAAIASAVGHPLRSPTWLAGGRPATRPLPGTGGDRAV"
BASE COUNT          450 a         1313 c         1337 g          455 t
ORIGIN      
        1 cgaccacgtc gccgtggcgc agctggagga acgtggcacc cgacccgtcg tcggcgctga
       61 acgcctggtc gccgaggtcg tcgcggtcgg tgacgatctg tccggcggcc tcggcggcgt
      121 ccgcggagcg ggtcacggca tcgtcggcgc ccgatgcgaa gcaggtgatg gtcgcgtaga
      181 cgaccgcctg tgcggtcgcc tcgtccgttg gctcgttccc gacgatcgtc atctgcttgc
      241 ggtcgacgtc gtactggctg gcgctgaccg tccagccggc gggcaggtcg cggtcccttg
      301 gcgtggcatt ccacgccgcg tcctggcacg agctgccggc cggcacgatc gcccgccacg
      361 cggccaggcc gagggcgacg agcgcgacga cgaccgcgat ccacgcgatc cgcctcgtgg
      421 tctgcgaggc ctgggcaagc tgcgcacgtt cctcggacgg ccgtggccgg ggctgggacg
      481 gcccgggttc cgggcgtggc gaacgggagg agctcatcgc ggtcgaggat gcccgatccg
      541 gatgagcgct gcctgagagc cgatcagcgt gcccgcattc ggttgttctt ggggttgtgc
      601 tcgagctcgg cgaggccgag cgcctcgagc agtggcggca ggtagacccc gaaccgcccg
      661 cgcagcccct tcttcaggcc gtaccagccg ccgaccgggt tcgcgtccga ccggccgaag
      721 gcctccacgg tcccgtcggc ggccggcttc tgctcgtccg cgccgcccag cggcacccag
      781 tcgccctggg ccttgagcca ggcgtgaaga tcgtcgatcg cgcgcaggtc gtagcgcagc
      841 tgcgtgccgc cgacctggca caggagcgcg ggcggatccg cggcctcgtc gcgccacatc
      901 tggtactgcg atcccccgtt ggccgtgacc agctgccacg ggtcctcgcg tgttccggcg
      961 cctgccatcg cgtcacctct gacttcgtgc ccggggtgag catacggcgc atcgcctcag
     1021 cggaggccct cggcgatcgc gatcgtggcg tcccgcccga gcgccgactc gaggcggtac
     1081 agcgtcccgt cgcgcgcgaa caccagcgtg tcgccggcca gcgcggacat caccatgtcg
     1141 acgtcgccgt ccggccgcgt cacgaaaatc tcgtgcgggg cgccggagat ccaccagcca
     1201 cggtcaccgc ccacgctcac cgactcgatc gaggagctgc gggcgaccag cttcttgagc
     1261 agcgcgtcgt tggtgccgcc cgggaccgcc atcagcgaga gcgccaggtc gctgctgggg
     1321 atcgtcggct ggctcgcctc ggcgcgccac gcgagcgtga cgatcctgcg atccccggca
     1381 ccgaggacgt aggcgacgtc gggctccggg aggccggccg gcagcaggac ccgcggctcg
     1441 tcgatggcgc gcgcctccgc gagcgggacc ggcgatccca ggtccaggcc cgagcccgcg
     1501 ggcggcccgg agctcggggg cggcagactc tccaccacca cgatctcgat gcccggcagc
     1561 cggaagccga gcgcgcccgc gatccccgcg aggaccagca gggtcaccag ggcgagcgcc
     1621 agggcggaca cgaggcggag cctcgtccga cgcgcggtcg gcgcggttct cgccccttcg
     1681 gcgcctccct cgatgcgagc cagcacgcgc gagctgagat ccggcgtcgc gggccagttc
     1741 gcgctcgccg ccgccatgcg cagcgctcct tccacgtcga cctccttcgc cgcgctcatc
     1801 gcgacgcctc ggcgagcgcg ccgatctggg ccaggtcatc gcgcagccgg gcgagggccc
     1861 ggtgcaggcg cgacttcacg gtgccggcgg ggatcccgag gaccgccgcg gtctccgcct
     1921 cggacagctc gagcaggtag cggcaggcga cgacctggcg ttcctcctcg cgcaggcgtc
     1981 cgagcgcctc gcgcacctcg gcgttggtct cgccggcgag cagggtgcgc tcgggggacg
     2041 gtgcgaccga cgacccgtcg tcgccgcgga acgcccgcac ggcgcggtcg agcatgcccg
     2101 cccggcggcc gagcgcccgg cggcggttcc gggcctcgtt gccgacaacc tggaggagcc
     2161 agggccggaa cggttcgccg gcccggaacc gcgcgagcgc gagataggcg cgaacgaagc
     2221 cctcctgggc cgcgtcctcg gcgtcggagg ccgagccgag cagcagatag gccacccgga
     2281 acgcggccgc ctcgtggatc gtcaccagct cgccgtacgc cgccgtgtcc cccgcctggg
     2341 cgcgccgaac gagggccgtc tcgatggccc cgggttcgtc cggcgcgcga ggctgcacga
     2401 aggcgtcagg cctccctgac ggggtcggag accggaagcc ccgcgaccag ccgggatcgg
     2461 cggctcgcga tgacggccgc agcgatgagc agcaccagag ccacgagacc cgccgcgagc
     2521 accatcggtg cgggcgacgg cgtggccgcg ggctgcgcgg tcggcgcgac cgccggcggc
     2581 gccacggccg gcttggcgac aaccggcggc ccggcggagg cgcgggcgat gggtgtgatc
     2641 ggggtcacga ccggtgcaac ctgggcggtc cgggccgtga tcgcacccgg gacgatggcg
     2701 gtgccggtga tcatgagcgg ccggtcggtg gacccgtcga agccgacctc gatgcccgcc
     2761 acgcccgacg cgggcacctc gacgcgcgcg gtgtagtggc cgcgcgcacc ctcctgcgcc
     2821 atcgcccggg tggcggcgcc gtcgggcccg atcaggccga cgtagagggt cgacccgtcc
     2881 accaggtgct gtgtgccatc gtagaggacc gtgaccgtca tcccgatcag gagcgtggtc
     2941 ccgccgggcg tgccacccgc gaccggtgcg tcgagctcgg cggccatccc ttccttggcg
     3001 aggaccgggg ccgcgagcgc ggtcgccccg aggacgaggg caccggcgat ggcgaggatg
     3061 cggtggggtc gatggctggg gttgcgcatg gctggcctcc tgggcgatgt ctgcgggtgt
     3121 tacaccgctc gatcgccccc ggttcccggc agcggccgcg tcgccggccg gccgccggcg
     3181 agccaggtcg ggctgcgcag gggatggccg acggccgagg caatcgcggc tgacgccgcc
     3241 atcacgccct cgaggctgac gccgtgctcg atgcccagcc cgtcgaggag gtacacgaga
     3301 tcctccgtgg cgaggttgcc gaccgcgccc gggccggcga acgggcagcc accgaggcca
     3361 ccggccgagc tgtcgacgat cgtcacgccg cgttcgagcg ccgcgaagac gttggccagc
     3421 gcctggccgc gggtgtcgtg cgcatggagg ccgacgcgcg cgacgtccac gatgggcagc
     3481 tcggcgtcga ggacccggtt gacgtcgccc ggcgtcgccg ccccgatcgt gtcaccgagg
     3541 ctgatctcgt cgcag
//
LOCUS       DBNHXO010000151         1628 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_830532, whole genome shotgun sequence.
ACCESSION   DBNHXO010000151 DBNHXO010000000
VERSION     DBNHXO010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_830532"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="AC162A_03135"
     CDS             <1..453
                     /locus_tag="AC162A_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR5009860.1"
                     /translation="LRVRIGARASVLLPQVRDAAAPRLSRAEPLMPIYEYLCTACSHR
                     ADILLGINDPGPQFCPACGREGTTRKQFAPPTIHFKGSGWAKKDRGSSTTRASSATSS
                     KAGESGGDGAAAKDTGESGGSKPASEASSGSTSSPSSTAGASSPKDGG"
     gene            455..694
                     /locus_tag="AC162A_03140"
     CDS             455..694
                     /locus_tag="AC162A_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR5009861.1"
                     /translation="MPSAADWITLAEAADILAAANVRFRPSTIGAWARSGRLSSIKLG
                     GRRYVRRGEVRALVTAPRRVHAEDVQPGLFEDMGS"
     gene            699..>1628
                     /locus_tag="AC162A_03145"
     CDS             699..>1628
                     /locus_tag="AC162A_03145"
                     /inference="COORDINATES: protein motif:HMM:NF015587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR5009862.1"
                     /translation="MSASEADGHVARIRALAVRIADRVGGLPMVKTLAGVLDAYDQGG
                     GGLVAGGLAFSALFALLPGLLLLLSVFGLVVKDQAVQAQLVTAITNAFPPLESFAETA
                     LTQVSAGAIPGGIIALVGLLWASSRFYSALDYAMARIFAQQKNRNEIQRTIRGLILTL
                     LLVAFPIAIVFAGAAIQALLDLLPDADTDGGLANLIVQLATPIGSIVLFVIATVMIYR
                     FVPAQRVPAAAWRRPAILIGLLLAGFTQLFAIFAPLMLRTAALYGAIVADFALLVWLS
                     IGFNMVLIGAAWTRVRAVALVSDALVGVEADPVE"
BASE COUNT          212 a          566 c          582 g          268 t
ORIGIN      
        1 ctgcgggttc gcatcggggc acgagcgagc gttctactgc cccaagtgcg ggatgcggct
       61 gctccgcggc tgagccgcgc cgagcccctg atgccgatct acgagtacct ctgcaccgcg
      121 tgcagccatc gcgccgacat cctgctcggg atcaacgatc cgggtcccca gttctgtccc
      181 gcgtgcgggc gcgaggggac cacgcgcaag cagttcgcac cgccgacgat ccacttcaag
      241 gggtcggggt gggcgaagaa ggaccgtggc agcagcacga cgcgggcatc cagcgcgacg
      301 tcgtccaagg cgggggagtc gggtggcgat ggcgcggcgg cgaaggacac cggcgagtcc
      361 ggcggctcca agcccgcgag cgaggcctcc tcgggcagca cgtcctcccc gtcatcgacg
      421 gccggcgcgt cctcgccgaa ggacggcggc tgaggtgccc tccgccgccg actggatcac
      481 gttggccgag gccgccgaca tcctcgccgc ggccaacgtg cggttccgcc cgtcaacgat
      541 cggcgcctgg gcccgatccg ggcggctgtc cagcatcaag ctcggtggcc ggcgatacgt
      601 ccggcgaggc gaggtccgtg cgctggtgac ggccccgcgg cgcgtccacg ccgaggacgt
      661 ccagccgggc ctgttcgagg acatgggctc ctgacgtcat gagcgcgagc gaggcggacg
      721 gacacgtcgc tcggatccgc gcgctggccg tgaggatcgc cgatcgggtc ggtgggctgc
      781 ccatggtcaa gaccctggcg ggcgtcctgg acgcgtacga ccagggtggc ggcgggctgg
      841 tcgcaggggg tctcgcgttc tcggccctgt tcgccctgct gcccggactc ctgctcctcc
      901 tctcggtgtt cggcctcgtc gtcaaggacc aggccgtgca ggcgcagctg gtcaccgcga
      961 tcaccaacgc gtttcccccg ctcgagagct ttgccgagac cgcgctgacc caggtgtcgg
     1021 ccggggcgat cccgggcggg atcatcgccc tcgtcggcct cctgtgggcg tccagccgct
     1081 tctactcggc gctggactat gcgatggccc ggatcttcgc ccagcagaag aaccggaacg
     1141 agatccagcg gacgatccgg ggcctgatcc tgacgctgct gctggtcgcg ttcccgatcg
     1201 ccatcgtgtt cgcgggcgcc gcgatccagg cactcctcga cctcctgccc gacgccgata
     1261 cggacggcgg gctcgcgaac ctgatcgttc agctggcgac gccgatcggc tcgatcgtgc
     1321 tgttcgtgat cgcgacggtg atgatctacc gattcgtccc tgcgcagcgg gttcccgccg
     1381 cagcctggcg acggccggcg atcctcatcg gcctgctgct ggccgggttc acgcagctgt
     1441 tcgcgatctt cgccccgctg atgctgcgaa cggcggccct ctacggggcg atcgtggccg
     1501 acttcgcgct cctcgtctgg ctctcgatcg ggttcaacat ggtgttgatc ggagcagcgt
     1561 ggacgcgtgt gcgcgcagta gcgctggtgt ccgatgcttt ggtcggggtg gaggcggacc
     1621 ccgtggag
//
LOCUS       DBNHXO010000152         2985 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2418349, whole genome shotgun sequence.
ACCESSION   DBNHXO010000152 DBNHXO010000000
VERSION     DBNHXO010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2418349"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="AC162A_03150"
     CDS             complement(<1..928)
                     /locus_tag="AC162A_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5009863.1"
                     /translation="MPGAIRAEGLAFRFASRPVFAFEDVSFAVEPGECLLVVGPSGSG
                     KSTLALALAGLVPREFPGEWRGRLTVDGLDPLTATGSEIAARVGLVFQDPESQLVMDR
                     VEDDVAFGLESRAWSRDAMLTRVPEALAEVGLGGLQRRRSTTLSGGQQQRLALAGALA
                     PRPGILVLDEPTANLDPPAARAFAARLAALRASRAVTIVLVEHRVDVAWPLADTVLAL
                     GPDGRPIDVGRPSEVLGRSRSRLVAAGAWLPDDDVAPPSPRPGHSTRASGPVVLTAHD
                     VSFAYDGPSVVRNVDFALTPWERVALIGTNGSG"
     gene            complement(942..1544)
                     /locus_tag="AC162A_03155"
     CDS             complement(942..1544)
                     /locus_tag="AC162A_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR5009864.1"
                     /translation="MATTVSSPGAAAPASRAWRTRDIVVTAIIGVTFGVVFWVWNIAW
                     GTMFESVFVFAPPAKDLLYAVWLIPAVLAPLIVRKPGAALFAEIVAAGVSAILGSQWG
                     VDALISGFVQGLGAEVVFALTLYRVWTFPVLAAAAVASAACAWVHDWVLYYADVSFEL
                     QLVRGLLMAVSAIVIAAGGSVLLVRSLRQAGVLEGFPADG"
     regulatory      complement(1600..1706)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1725..2594)
                     /locus_tag="AC162A_03160"
     CDS             complement(1725..2594)
                     /locus_tag="AC162A_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HIR5009865.1"
                     /translation="MTEAPAPPFTPSIATARMADGTILRTLHWPAPDDPWATALVVHG
                     VGEHGGRYANVARPLVAAGVDVNSYDQRGFGGSAGPRAYVERWSDFHDDLQERLTSLR
                     ESDPDRPFILYGHSLGGLIATGYVLSERPRPVPDLLVLSSPALEDAIPAWKRSMAGGL
                     VRVVPRMKMSNGGMGDELSHDPAISAAYVNDPLCLDSSTVRLGYEAFAEQARLRVAID
                     AREAMPMPTYVFHGTADRIVPVSASEAIGRKGNVTRVVYKDLRHETHHEFAHEDVLAG
                     VVGWLEGQRSARV"
     gene            2657..>2985
                     /locus_tag="AC162A_03165"
     CDS             2657..>2985
                     /locus_tag="AC162A_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5009866.1"
                     /translation="MSQRWTEIGDRVFVRRYEFFDQDIVAVLGGSGALVVDTRSTPAQ
                     AREILDDLRDLGSPRVGIVVNTHGHFDHAFGNAVFRPAVIWGHERCAAMVRLTGELQR
                     TAVADEMP"
BASE COUNT          463 a         1095 c         1059 g          368 t
ORIGIN      
        1 tgcccgaccc gttggtgccg atcagggcta cccgctccca cggcgtgagc gcgaagtcga
       61 cgttgcggac gaccgatggg ccgtcgtagg cgaacgacac gtcgtgggcg gtgaggacga
      121 cggggccaga ggcgcgggtc gagtgcccgg gccggggcga cggcggcgcc acgtcgtcgt
      181 cgggcagcca ggcgccggcg gcgacaaggc gcgatcgcga cctccccagc acctccgacg
      241 gccgcccgac gtcgatgggc cggccatccg gcccgagggc cagcacggtg tcggcgagcg
      301 gccacgcaac gtcgacccgg tgctccacga ggacgatcgt cacggcccgc gacgcgcgca
      361 gcgcggcgag ccgggccgcg aacgcgcgcg ccgccggcgg gtcgaggttc gcggtcggct
      421 cgtcgagcac gaggatcccg ggccgcgggg cgagcgcgcc ggccaacgcg agccgctgct
      481 gctggccgcc ggacagcgtc gtcgaccgcc gccgttggag cccgccgagg cccacctccg
      541 caagcgcttc cgggacccgg gtgagcatcg cgtcgcggga ccaggcacgg ctctcgagcc
      601 cgaacgcgac gtcgtcctcg acccggtcca tgaccagctg gctctcgggg tcctggaaca
      661 cgagaccgac cctcgccgcg atctcggaac ctgttgccgt cagcgggtcg aggccgtcga
      721 ccgtcagccg gcctcgccac tcgccgggga actcgcgcgg cacgaggccg gcgagggcga
      781 gcgcgagcgt gctcttgccc gaccccgacg gcccgacgac gagcaggcac tccccgggct
      841 cgaccgcgaa cgagacgtcc tcgaacgcga agaccgggcg gctcgcgaac cggaaggcca
      901 gcccctcggc gcggatcgcg ccgggcaccc ccggggccgg ctcagccgtc agctgggaac
      961 ccctccagca cgccggcctg acgcagcgag cgcacgagca gcaccgagcc gccggcggcg
     1021 atcacgatcg cggacaccgc cattagcagc ccgcgaacca gctggagctc gaacgacaca
     1081 tctgcgtagt acagcaccca gtcgtggacc caggcacatg ccgcggaggc gacggccgca
     1141 gccgcgagca ccgggaacgt ccatacgcgg tagagcgtga gcgcgaacac gacctcggcg
     1201 ccgaggccct ggacgaagcc cgagatcagc gcatccacgc cccactggct gcccaggatc
     1261 gccgagaccc cggccgccac gatctcggcg aacagtgcgg caccgggctt gcggacgatc
     1321 aggggcgcca acacggcggg gatgagccag accgcgtaca gcaggtcctt ggccggcggc
     1381 gcgaacacga acaccgactc gaacatcgtg ccccaggcga tgttccagac ccagaacacg
     1441 acgccgaagg tgacgccgat gatcgccgtc acgacgatgt cgcgcgtccg ccatgcccgc
     1501 gacgcggggg cggccgcgcc cgggctcgag accgtcgtcg ccattgaaat cgtgacctcc
     1561 atcaagcacg aacgccgccc tcgatgccgg gggcggcgtg ctgcgttgcg ggcctgcttc
     1621 ctacaccggt acgaaccgga tcaggttccg cgggtctgtg gtctgcacca cactctcagc
     1681 gctcgcgcgc tccccggagg cggatcttcg attgtcggcc gagtctacac ccgggcggat
     1741 cgctgaccct cgagccagcc gaccacgccg gcgagcacat cctcgtgcgc gaactcatgg
     1801 tgcgtctcgt ggcgcaggtc cttgtagacc acccgggtca cgttgccctt gcggccgatc
     1861 gcctcggacg cggacaccgg cacgatccgg tcggccgtcc catggaagac gtaggtcggc
     1921 atcggcatgg cctcgcgggc gtcgatcgcg acgcgcaggc gggcctgttc cgcgaacgcc
     1981 tcgtagccga gacggacggt ggaggagtcc aggcacagtg ggtcgttgac gtacgcggcg
     2041 ctgatcgccg gatcgtgcga gagctcgtcg cccatgcccc cgttcgacat cttcatccgc
     2101 ggtaccacgc gcaccagccc acccgccatc gaacgcttcc aggccgggat ggcgtcctcg
     2161 agcgccggcg acgagaggac cagcaggtcc ggcaccgggc gtggtcgctc cgacaggaca
     2221 tagcccgtcg cgatcagccc gcccagcgag tggccgtaga ggatgaacgg gcgatccggg
     2281 tcggactcgc ggagcgacgt gagccgctcc tggaggtcgt cgtggaagtc gctccagcgc
     2341 tcgacgtagg cccgcggccc cgccgagccg ccgaacccgc gttggtcgta gctgttgaca
     2401 tcgacgccgg cagcgacgag gggccgggcg acgttcgcgt agcgaccacc gtgctcgccc
     2461 acgccgtgga cgacgagcgc ggtcgcccac ggatcgtcgg gcgccggcca gtggagcgtg
     2521 cgcaggatgg tcccgtccgc catcctcgcc gtagcgatcg acggcgtgaa cggcggggcc
     2581 ggtgcttcgg tcatcaacga ttcctcgtgc gcggcggagc gtactcgttc ggcgcacccc
     2641 tcggtacgat gacagcgtga gccagcgatg gaccgagatc ggcgaccggg tcttcgtccg
     2701 gcgctacgag ttcttcgacc aggacatcgt cgcggtcctg ggcgggtccg gcgcccttgt
     2761 cgtcgatacg cggtcaacgc cggcgcaggc gcgcgagatc ctcgacgacc tgcgcgacct
     2821 cggctcgccg cgcgtcggga tcgtcgtcaa cacccacggc cacttcgatc acgcgttcgg
     2881 caacgccgtc ttccggccgg ccgtgatctg gggccacgag cggtgtgccg cgatggtccg
     2941 gctcaccggc gagctccagc gcacggcggt cgcggacgag atgcc
//
LOCUS       DBNHXO010000153         2185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1427907, whole genome shotgun sequence.
ACCESSION   DBNHXO010000153 DBNHXO010000000
VERSION     DBNHXO010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1427907"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..1666)
                     /locus_tag="AC162A_03170"
     CDS             complement(239..1666)
                     /locus_tag="AC162A_03170"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR5009867.1"
                     /translation="MKLAPTTTSTDAALDRAAEIAREFIAGLPDRPVAPTVSVDELRR
                     RFGGPLPEAGLEPVDVVDALAANAEGGLIGSAGPRFFGFVIGGGVPAAVAADWLTSAW
                     DQNAGLYVIGPAAAVAEEVAGAWLAELFGLPAAVSVGFTTGATMASFTGIAAGRHALL
                     ARGGWDVERQGLFGAPEFPVVVSDESHVTIFAALQMLGMGRDRVIRVPTDDQGRMRPD
                     ALREALGQLDRPALVCAQAGNVNTGAFDPLPPIVAAVRANGGWLHVDGAFGLWAAADP
                     ARRHLVDGVGEADSWTTDAHKWLNVPYDSGLSFVADPAAHHAAMTLGAAYYVETTGAE
                     RDAYNWVPESSRRARGFPMWAALRQLGRSGVAEMISRTSDHAQRFAAGLADAPGVRIL
                     NEVVLNQVLVRFDDPSGAAQAGDARTDAVIAAIQADGTLWLGGTRWHDLRAMRISVSG
                     WSTSEADVDRSIAVIRRVAEGIVAG"
     gene            1747..2109
                     /locus_tag="AC162A_03175"
     CDS             1747..2109
                     /locus_tag="AC162A_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5009868.1"
                     /translation="MTHDDANRWLQAYVEAWRSYEPDAIGALFADDARYRYHPWDEAI
                     EGREAIVADWLADRDDPDLWQADYEAWAVEDDRVVATGSSRYDDPSGKRTYHNVFLLR
                     FAADGRCREFTEVYAQER"
BASE COUNT          317 a          813 c          772 g          283 t
ORIGIN      
        1 cgaggcggca cgccttcgcg cgcccccgcg gcggcggcct ctggaacccc agacgttcac
       61 tgcagcggtc cgcgggcccc agctccccca aattggtagc ccaggcgttc cgcatggccc
      121 gatcgacggc cgattggaac gccacgggtt cagatcggcc gctgcttggc tctggcgcag
      181 cgcgagtgaa cctctcgcgt tccaagcacg ccggcatccc gggagcggcg ctgatctgct
      241 acccggcgac gatcccctcc gccacgcgac ggatcaccgc gatcgaccgg tccacgtccg
      301 cctcggacgt tgaccatccg gagaccgaga tccgcatcgc ccgcaggtca tgccagcggg
      361 tgccgcccag ccacagcgtc ccgtcggcct ggatcgccgc gatcaccgcg tcggtccgcg
      421 cgtcgcccgc ctgtgccgcg cccgatgggt cgtcgaaccg gaccaggacc tggttgagca
      481 cgacctcatt gaggatccgc acgcccggcg catccgcgag ccccgcggcg aaccgctgcg
      541 cgtggtccga ggtccggctg atcatctccg cgacgccgct ccgcccgagc tggcgcaggg
      601 ccgcccacat cgggaacccc cgggcccggc gcgacgactc gggcacccag ttgtaggcgt
      661 cgcgctcggc gcccgtcgtc tcgacgtagt aggccgcgcc cagcgtcatc gccgcgtggt
      721 gggccgccgg gtcggcgacg aacgagaggc ccgagtcgta ggggacgttg agccacttgt
      781 gggcgtcggt ggtccaggag tcggcctccc ccaccccgtc gacgagatgg cgacgggccg
      841 gatccgcggc ggcccacagg ccgaaggcgc cgtcgacgtg cagccagccg ccgttcgcgc
      901 ggacggccgc gacgatcggt ggcagcgggt cgaatgcccc ggtgttgacg ttgcccgcct
      961 gggcgcacac gagcgccgga cggtcgagct ggcccagcgc ctcgcgcagc gcatccgggc
     1021 gcatccgacc ctggtcgtcg gtggggacgc ggatcacgcg atcgcgcccc atgcccagca
     1081 tctggagggc ggcgaagatc gtgacgtggg actcgtcgct gacgacgacc gggaactcgg
     1141 gcgccccgaa caaaccctgg cgctcgacgt cccagccgcc ccgggccagc agcgcatgcc
     1201 ggcccgcggc gatcccggtg aacgaggcca tggtcgcgcc ggtcgtgaac ccgacgctga
     1261 cggcggccgg cagtccgaac agctcggcca gccacgcacc ggcgacctct tcggcgacgg
     1321 cggccgcggg cccgatgacg tacagacccg cgttctgatc ccacgcgctg gtcagccagt
     1381 cggcggccac cgctgcggga acgccgccgc cgatcacgaa cccgaagaag cgcggaccgg
     1441 cgctgccgat gagcccgccc tcggcgttcg cggccagggc gtccacgacg tcgacgggct
     1501 cgagcccggc ctccggcagc ggtccgccga accggcgccg gagctcgtcg accgacaccg
     1561 tcggcgcgac cggccggtcc ggcaacccgg cgatgaactc gcgggcgatc tcggcggcgc
     1621 gatccagggc cgcgtcggtg gaggtggtcg tcggggcgag cttcatgact ggtccggcct
     1681 cgttgtaact ggactgagcc ttgtggtagg ttacacgggt tcggcaagcc accacctgag
     1741 gttccgatga cgcacgacga cgccaatcgc tggctgcaag cctacgtcga agcctggcgc
     1801 agctacgagc cagacgccat cggcgcgctg ttcgcggacg acgcgcgcta tcgctaccac
     1861 ccctgggacg aggccatcga gggccgcgaa gccatcgtgg ccgactggct cgccgaccgc
     1921 gacgacccgg acctctggca ggcggactac gaggcttggg ctgtcgagga tgaccgtgtg
     1981 gtggcgaccg gcagcagccg gtacgacgac ccgagcggca agcggacgta tcacaacgtg
     2041 ttcctgctca ggttcgcggc cgacggccgg tgccgcgagt tcaccgaggt gtacgctcag
     2101 gaacggtgat ccggatcggc gcacgctcgg ccgccacatg cctcgtggtc gtcgccgcgc
     2161 tgggcgtctc ggtcggcgga cccga
//
LOCUS       DBNHXO010000154        15812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1229518, whole genome shotgun sequence.
ACCESSION   DBNHXO010000154 DBNHXO010000000
VERSION     DBNHXO010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 15812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15812
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1229518"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="AC162A_03180"
     CDS             <1..465
                     /locus_tag="AC162A_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HIR5009869.1"
                     /translation="PSSRLLAFGVTAADVGLAARLRVAVGERVVGFTRLRLGNDEPMA
                     VESVWIAAALVPGIAPDDLGGSLYELLAQRYGVVAGAANVTIEPILPDAETRAHLAIP
                     DDQACLRLRMIDSDGRGRVVMAADCTYRGDRYQLSANVSGAAFSSLTDRRAG"
     gene            465..1397
                     /locus_tag="AC162A_03185"
     CDS             465..1397
                     /locus_tag="AC162A_03185"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HIR5009870.1"
                     /translation="MHVLGVDGGQSGIRMRHSDDERTVEVDGVSRLEGDTVAQLADAV
                     AEAHRSEGFPPADRVVLGLTTAPSDADAIDRLCGLVAEATGAPEVWLADDAVTSHAGA
                     LSGGWGVSLISGTGVACLAVPDDGEPRIIGGHGYLLGDEGGGFWIGRRGLGAVLRAGE
                     GRGSATDLTDSARRRFGELEGLHVRLHDSARPVHEIASFAPDVLEAAEAGDEVAGALL
                     DRAADELALTAWSGATWAAGSSTTGAVPLALGGRLLADDGPLRRRLDERLAQLRSPVA
                     VRTADGSPLDGALLIGGRDDPGPYRTLVHVWSGR"
     gene            1394..2131
                     /locus_tag="AC162A_03190"
     CDS             1394..2131
                     /locus_tag="AC162A_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR5009871.1"
                     /translation="MSDPLASPGRRYLEIAGELVARLLDDEWPNIRAAAELVADTLAA
                     RGIVHAFGTGHSHMLAEELYYRAGGLVDVRPILFEGLMLHASAPLSTTLERLPGLGAA
                     LLEDHPIHPGDALIVASNSGSNAVTSELAQLARDAGARVIAVTSLRHATSSAARATAL
                     PRLHELADVVIDNGGCVGDAAVEIRGVGSRVAPTSTVVGAAIVNAIEAEAVGILVERG
                     AAPEIFASSNVEGGDAVNAALLGRRAP"
     gene            2139..3512
                     /locus_tag="AC162A_03195"
     CDS             2139..3512
                     /locus_tag="AC162A_03195"
                     /EC_number="3.2.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102571 -
                     [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine/L-
                     threonine O-N-acetyl-alpha-D-glucosaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009100 - glycoprotein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HIR5009872.1"
                     /translation="MTSPASVSPFAVRGVLEGFYGNPWTHEQRLDLIAFIASRGMNTF
                     VYTPKDDPLMRRDWRSTYEGVELRRLTELVNRCRASGVDFVFCVSPGLTMRYSDDDDV
                     EALCDKLASIASLGVTSFGLLFDDIPPDLQHAEDREAFDSLADAHLGVANAVFERLPR
                     GSRLTVCPTTYWGTGTEPYLATLGGGLEPGIDLFWTGRAICSPTLDVRDAATFERTAR
                     RPATYWDNYPVNDVAMGFELHIGPYRGRDPHLFRHAFGVVANGMELYEASKIPIATIA
                     DYLMAPEKYDAEKSWRRAMRDVVGDRDVDDFALFADNVRSSCLSPDDAPAVTRALETF
                     AFQMDQGAGRLAASELGTLANRLLAAADHLLRGPVENRALIAECRPWIEAFETGAQAL
                     RQIAVLAAEGRLEKDAAELRPYLVRLRQARVRVFGDALDMTLADLTNTHVRPGELTLD
                     APGGGAA"
     gene            3509..4774
                     /locus_tag="AC162A_03200"
     CDS             3509..4774
                     /locus_tag="AC162A_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5009873.1"
                     /translation="MTRSIRGLAALATIVVLAIAGCAGTSAPSNDHVLRVAMGSPGEA
                     QIRVWDDVAKQFEAANPGTKVEMNYQDDDLYQTIGLPNLLNGRNAPDIYFEWTGNRLE
                     QRVKDGFGADISEAFKTGPLAGLFDDATLAQYKVDDKVVMVPYSADVTNVLWFNKKLL
                     ADAGVEPPTTWEELLAACDALNAKGITPIASGNKDLWAAGNWLSHMVSRVVGEDAYAA
                     VLSGTGKFDTPEWEKAFGYIKELADHKCVNDSANSIADDAGAQLFFGGQAAMHPIGSW
                     LVSWAIDEAPDLDFDYVNLPAMPGGTGNQDSVIGVATGYVVNAKSAKIPEATSFMALL
                     NNEANVQALLEAEVTPLTKSASAGTDIDSRSARLSTLLNEAPAIVLPPDTGYDLKMAD
                     ALYAAEAAVLGGQQTPKEALAAIDAKLGR"
     gene            4846..5715
                     /locus_tag="AC162A_03205"
     CDS             4846..5715
                     /locus_tag="AC162A_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5009874.1"
                     /translation="MKPTRAVTPYLFLAPAVAVFGFAVLVPMLLTIGYSLTEWNGFGP
                     MTFVGLDNYLAAASDAVFRDSFVHVLGYIAATLLLEVGLGLGLAGLVSIRRGGLWFRV
                     AIFAPVMLPMVVVAVLWSFVYNADFGLINAGLGAIGLEGLQRVWLGDTSTALLAVSVV
                     SGWVYAGFYMVIFYAAFSQIPSEVLEAARMDGAGEWALFRRIKVPMIRNVVAVAILLC
                     VTGGFQGFDLFFVLTNGGPYGATEIPTTYLVKVVFRNGDVGYGSAMAVVLTVVVLAVG
                     LAYARLHRRGSTS"
     gene            5712..6536
                     /locus_tag="AC162A_03210"
     CDS             5712..6536
                     /locus_tag="AC162A_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5009875.1"
                     /translation="MRRRSLPDLLVLAGMVLLAAVFFLPMVWMVISSFKSNSEIFQSP
                     FALPTQVDLGRWAEAWEVGHLGQYALNSAITTAVSVFLILVLGASAAFAFSRYRFRGR
                     GFLLSLFALGLILPLQSYFIAQSTLFNQLAITDTRLALIIPYAAMGLSLAVYLLKVYL
                     DALPDELFDAARIDGAGDARVFWLLALPLLRPGLATVAIFSALAAWNEFLLALLYIQD
                     DALKTIPTGLLAFSSRYVTDYSLLFSALTIVTLPMILIYIVFNRHIVEGITAGSVK"
     gene            6659..7291
                     /locus_tag="AC162A_03215"
     CDS             6659..7291
                     /locus_tag="AC162A_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009876.1"
                     /translation="MSESETENSQPVRTRRAILGAAAAGAVGLSVGRFVRPETASAAT
                     GAMQYGADNNAGDAGTILRSTYEFQALEVHGTGANAIAGLGEGAESTGVIGVGSGPGF
                     GVRGVSNHGVGLHGESVDGYALKAWGRAGFSTAGTATVARHKASVKVKRDEVSPGSLV
                     IATIQRDRADTYVRGVVVDDGSFRVLLNRAVASPTKVAYIVIEKLEPFPD"
     gene            complement(7304..8629)
                     /locus_tag="AC162A_03220"
     CDS             complement(7304..8629)
                     /locus_tag="AC162A_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014686280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5009877.1"
                     /translation="MDTTSATSHPDQPPPGLAAAPVEIPPLMKQRDFRALLGARFTNS
                     LAMSALVTVVGFQVYAMTGDPLALGWLGLVEAIPALSLVLFGGYVADRHDRRRIIVIT
                     SALVTGCAAALGLLAADGASGILPILAVVFVTGIGSGFERPALTAFEAQVIPRVQAVQ
                     GVSYTSSVSQAGGILGPLTGGIALVVIGIAGTYAAMAVLLAISTVCLALIPRRPMPEI
                     VAGEPRLQSLLDGIRYVRRTPALLGSMALDLFAVFFGGAIALLPVFATDVLHVGPIGL
                     GIMRTAPSIGALGVMLIATRRPPSRHAGRTLLICVAGFGASMIVFGLSTTFALSVLAL
                     FVSGVTDGLSMVIRQTILRVLSPERIRGRVASVNWIFIGASNELGAFESGFAAHAFGT
                     VPSVVGGGVLTLGVVGLVALAVPSLRGLDLDRAEPVDDGPPEASSALAA"
     gene            complement(8857..9234)
                     /locus_tag="AC162A_03225"
     CDS             complement(8857..9234)
                     /locus_tag="AC162A_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009878.1"
                     /translation="MTEFTQPTTQPAAPDAGPSRLNGGRRAIASVLLTVGLFALGGTA
                     VALAADPSASPAPSTTPTTPTQPSDGGTGAPSNGGTTAPSGRPSHAGKDCPNMGDDSA
                     GSGGSGASPTTPTTPDDTATPSV"
     gene            9496..9729
                     /locus_tag="AC162A_03230"
     CDS             9496..9729
                     /locus_tag="AC162A_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009879.1"
                     /translation="MSGIASGLLVWAYLLIWDATDFTTPFTAYFFAGVVAALGGWIWP
                     VVIGWFLVRRHRSKQDEQIQKEVDRQIAEKSGQ"
     gene            9843..10034
                     /locus_tag="AC162A_03235"
     CDS             9843..10034
                     /locus_tag="AC162A_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009880.1"
                     /translation="MTARLFIPTPLRLIAILLTLVVVATLEASLAGPALGATQPLSRG
                     KLVETSGCTEILISANVIK"
     gene            complement(10042..10392)
                     /locus_tag="AC162A_03240"
     CDS             complement(10042..10392)
                     /locus_tag="AC162A_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5009881.1"
                     /translation="MAQSIGSVLKQARDVRELSGVDAARAAGISPAYLSKLESDAVKR
                     PSPHVLHQLSEALGLPYAELMRLCGYRVPGDAEPGDARAVSAALFADLSDDEREELIE
                     YLAWYRARRRNGRR"
     gene            10487..11122
                     /locus_tag="AC162A_03245"
     CDS             10487..11122
                     /locus_tag="AC162A_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009882.1"
                     /translation="MPLPLLIIAVAAMTGLGVLRVVRVHLDRTPLPEGRARTLFKIAF
                     LVVPPVVLGVPAGAPLGGLAWVPLYAVILGGLLVLVTIAAAIVERVAKGRTRQVLMLA
                     LVGSEGDPEDLPFDPPVTARLAESMAIVDRANAQFPRGLAFPSQVDRADFRSAWGTLD
                     DATRVLEGHIVEDRRLGLGTAAAASVTAADARGRLDTLVSIALRRGLAAPA"
     gene            11128..11454
                     /locus_tag="AC162A_03250"
     CDS             11128..11454
                     /locus_tag="AC162A_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009883.1"
                     /translation="MAELTPHPVAQMARRIPAAARATRTGAQSTTSALQRLPDSSLRW
                     LAAASIGLAAGLQISGAPRLTRWVGMAPALVIGAALALRPPEPVATAAVLELVPAHAP
                     GEVRSS"
     gene            11451..11654
                     /locus_tag="AC162A_03255"
     CDS             11451..11654
                     /locus_tag="AC162A_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009884.1"
                     /translation="MTPLQNQAITTLPNVIRDQTMPNTLGGVSDPSPFLPRRAPSVAS
                     AVVAAGLVATVIAAVLAIGGRFA"
     gene            complement(11669..12061)
                     /locus_tag="AC162A_03260"
     CDS             complement(11669..12061)
                     /locus_tag="AC162A_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HIR5009885.1"
                     /translation="MAYVRDPRVDAYIDALPDWQQEICHRVRDLVHDADPEVAETIKR
                     TVQPYFVLNGNICALLAAKDHVNVFLYDGGIVPDPEGIVTAGHDNTTARTVAFRRGEA
                     INEPALSAMFRQIIANNRAGGWRRLKGG"
     gene            complement(12525..13037)
                     /locus_tag="AC162A_03265"
     CDS             complement(12525..13037)
                     /locus_tag="AC162A_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009886.1"
                     /translation="MRIKLVTPILLIALVAACGGGGTASTTKPAATNAEAPADTAAPA
                     GNGSVDCVKVKAAATKLIVAVQLLAQLRDPANVEAVKSGTVGNLDLDEFLAALKDLHA
                     LDGQQSVLGDPKTAIEAYEKAGEAAKVLFATDPVTQDAVDTYNTNVGTIPAFLGHQGA
                     IAGAMDAAGC"
     gene            complement(13100..13690)
                     /locus_tag="AC162A_03270"
     CDS             complement(13100..13690)
                     /locus_tag="AC162A_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009887.1"
                     /translation="MTTVSLADASSCCGLDLDVAGRRASGAAAGLTIGAAIALGVFFA
                     AGEPWGTINDSLSIALAWATVPIAVQLTRREPPSRALSLGAACDVVGVAVITVFTTLL
                     ISRRMTFEESLMPIMSGQSLIGTWLILVGLSAWPDRRSRRAAASALLGGAGLVATNAG
                     LATGGMGSPVALAGFVAGLIGTTAFYAQLARRRSRP"
     gene            complement(13722..14231)
                     /locus_tag="AC162A_03275"
     CDS             complement(13722..14231)
                     /locus_tag="AC162A_03275"
                     /inference="COORDINATES: protein motif:HMM:NF021538.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HIR5009888.1"
                     /translation="MTWAGLYPWLLVLHVLSAFAFLAVHGVSMGVWWRVRRERDRTKL
                     ASLLDLSAAFITPMMLAGLLLIVSGILVGIAAGWWFNGQWWLWASIGVLIVIVALMTP
                     LLSIPMGGVRRGLGMPSQADTKAGVVPRPVDDAALERLLSDPRPTIGGSIAIAGIVVI
                     TWLMEVKPF"
     gene            complement(14244..15014)
                     /locus_tag="AC162A_03280"
     CDS             complement(14244..15014)
                     /locus_tag="AC162A_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5009889.1"
                     /translation="MRTFAMRLVGISYAAIIAVFVVYATQPFAADFLAVIAGFIVLFG
                     CVGLASVAWSGARTRPWFWLVSSALGILVLLFNGFYGPYALAHPADALTFVTTLVVLV
                     AGALVVVGSLTAWLEVRRQRPLWEQRGRAGFVAAGLAGLVAGACVTSVVAASAAPVGV
                     ALDQPPATTVALTARDTKFLETGLEARSGDVLGVFVTNKDGFAHSFDVDALGIHVPLP
                     ADSTTFVAVKPVGAGQLPFYCAVPGHQDAGMVGAISVH"
     gene            15127..15714
                     /locus_tag="AC162A_03285"
     CDS             15127..15714
                     /locus_tag="AC162A_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5009890.1"
                     /translation="MPRKYDLGRRAEQQADTRRRIIDAAMELYQEQGASGTTMLDVAR
                     RADVAPGTVANHFGSAAALAAVVTDQVLSDLHVPTPDLFDGVEDLSDRIEVLVRELAA
                     FMDRSETWWKVTQREPAGAQVWADAQARYYAELDTLVRAALGPLAADADAVAVVTTIL
                     GTWVIGAIQATGRSAETAVALVSDLLATWLATRTT"
BASE COUNT         2328 a         5650 c         5564 g         2270 t
ORIGIN      
        1 ccgagcagtc gcctgctggc gttcggcgtg acggccgccg atgtcggcct cgcggcgcgc
       61 ctccgcgtcg cggtcggcga gcgcgtcgtg ggcttcacgc gcctgcggct cggcaatgac
      121 gagccgatgg cggtcgagtc ggtctggatc gcggccgccc tggtgcccgg catcgcgccg
      181 gacgacctcg gcggctccct gtacgagctt ctcgcccagc gctacggcgt cgtcgcgggt
      241 gccgcgaacg tcacgatcga accgatcctc ccggacgcgg agacgcgggc ccacctcgcg
      301 atccccgacg accaggcgtg cctgcgcctg cggatgatcg actcggatgg ccgcgggcgc
      361 gtcgtgatgg ccgccgactg cacctaccgc ggggaccggt accagctcag cgccaacgtg
      421 tcgggcgccg ccttctcgtc gctcaccgac cggcgggccg gctgatgcac gtgctgggtg
      481 tcgacggcgg ccagtcgggg atccgcatgc gccactcgga cgatgagcgg acggtcgagg
      541 tcgatggggt cagccgcctc gagggcgaca ccgtggcgca gctcgccgac gccgtcgccg
      601 aagcccatcg ctcggaaggc ttcccgcccg cagatcgcgt ggtcctgggc ttgaccaccg
      661 ccccgtcgga tgccgacgcc atcgaccggc tgtgcgggct cgttgccgag gccaccggag
      721 cgcccgaggt ctggctggcc gacgacgccg tgacctccca cgcgggcgcg ctgtcgggcg
      781 gctggggcgt cagcctgatc tccggcacgg gcgtcgcgtg cctggcggtc cccgacgacg
      841 gcgagccccg gatcatcggc gggcacggct accttctggg cgacgagggc ggcgggttct
      901 ggatcgggcg caggggcctg ggcgccgtgc tccgggccgg cgagggccgc ggctccgcga
      961 cggacctcac ggacagcgcc cgacggcgct tcggcgagct cgaggggctc cacgtgcgcc
     1021 tgcacgacag cgcgcgaccg gtccacgaga tcgcgagctt cgcgcccgac gtcctcgagg
     1081 cggccgaggc cggcgacgag gtggccggcg cgctcctcga ccgggccgcg gacgagctcg
     1141 cgctcacggc ctggtccggc gcgacctggg cagcagggtc ctcgaccacc ggggccgtgc
     1201 cgctcgccct gggcggccgc ctgctcgccg atgacggtcc cctccgccgc cggctcgacg
     1261 agcggctcgc gcagctccgc tccccggtcg ccgtccggac ggccgacggc agcccgctcg
     1321 acggcgcgct cctcattggc gggcgggatg acccggggcc gtaccgcacg ctcgtccacg
     1381 tctggtccgg tcgatgagcg acccgctcgc ctcgcccggc cgccgctacc tcgagatcgc
     1441 cggcgagctc gtcgcccgcc tgctcgacga cgagtggccc aacatccgcg cggccgcgga
     1501 gctggtcgcc gacaccctgg ccgcgcgggg tatcgtgcac gcgttcggca ccgggcactc
     1561 ccacatgctc gccgaggagc tctactaccg cgccggcggg ctggtcgacg tccgcccgat
     1621 cctgttcgag ggcctgatgc tccatgcgag cgcgccgctc agcaccacgc tcgagcggct
     1681 gcccgggctc ggcgccgcgc tcctggagga ccacccgatc caccccggcg acgcgctcat
     1741 cgtcgcctcc aactcgggca gcaacgccgt cacctcggag ctggcgcagc tcgcccggga
     1801 cgccggcgcg cgcgtgatcg ccgtgacgag cctgcgccac gcgacctcgt cggccgcccg
     1861 cgccacggcc ctgcccaggc tccacgagct cgcggacgtc gtgatcgaca acggcggctg
     1921 cgtcggcgat gcggcggtcg agatccgcgg cgtcggcagt cgcgtggcgc cgacgtcgac
     1981 ggtcgtcggc gcggcgatcg tcaacgcgat cgaggccgag gccgtcggga tcctcgtgga
     2041 gcggggcgcg gcacccgaga tcttcgccag cagcaacgtc gagggcggcg atgcggtgaa
     2101 cgcggccctg ctcggacgcc gggcaccgtg aggagccgat gacgtccccg gcctcggtct
     2161 caccgttcgc cgtgcgcggc gtcctcgagg ggttctacgg caacccatgg acccacgagc
     2221 agcgcctgga cctgatcgcg ttcatcgcct cgcggggcat gaacacgttc gtctacacgc
     2281 ccaaggacga cccgctgatg cgccgggact ggcgctcgac ctacgagggc gtcgagctgc
     2341 ggcggctcac cgagctcgtc aaccggtgcc gcgcgagcgg cgtcgacttc gtgttctgcg
     2401 tgtcgcccgg cctgacgatg cgctattccg acgacgacga cgtcgaggcg ctgtgcgaca
     2461 agctcgcgtc gatcgcctcg ctcggcgtga ccagcttcgg gctcctgttc gacgacatcc
     2521 cgcccgacct ccagcatgcg gaggaccggg aggcgttcga cagcctcgcg gatgcgcacc
     2581 tgggcgtcgc caacgccgtg ttcgagcgcc tcccgcgggg ctcgcggctc accgtctgcc
     2641 cgaccacgta ctggggcacc ggcaccgagc cctacctcgc cacgctgggc ggcggcctcg
     2701 agcccggcat cgacctgttc tggaccggcc gcgcgatctg ctcgccgacg ctcgacgtcc
     2761 gcgacgcggc gacgttcgag cgcaccgccc gccgccccgc gacctactgg gacaactacc
     2821 ccgtgaatga cgtggcgatg ggcttcgagc tgcacatcgg cccgtaccgt ggccgcgacc
     2881 cgcacctgtt ccgccacgcg ttcggcgtcg tcgccaacgg gatggagctc tacgaggcat
     2941 ccaagatccc gatcgcgacc atcgctgact acctcatggc acccgagaag tacgacgcgg
     3001 agaagagctg gcggcgggcg atgcgcgacg tcgtcggcga tcgcgacgtc gacgacttcg
     3061 ccctgttcgc ggacaacgtc cgctcgtcct gcctcagccc ggacgatgca ccggccgtga
     3121 cccgggccct cgagaccttc gccttccaga tggaccaggg cgcggggcgc ctcgccgcat
     3181 ccgagctcgg gacgctcgcc aaccgcctgc tcgcagccgc ggaccacctg ctgcgcgggc
     3241 cggtcgagaa ccgcgccctc atcgccgagt gccggccgtg gatcgaggcg ttcgagaccg
     3301 gcgcgcaggc gctgcgccag atcgccgtgc tggccgcaga gggccgcctg gagaaggatg
     3361 cggcggagct ccgcccatac ctcgtgcgac ttcgccaagc gcgggtccgc gtgttcggcg
     3421 acgcgctcga catgaccctc gcagacctca ccaacaccca tgtcaggcca ggagagctga
     3481 cgctcgacgc accaggagga ggagcagcat gaccaggtca atccgggggc tcgccgccct
     3541 cgcaaccatc gttgtcctcg caatcgccgg ctgcgccggc accagcgctc cgtcgaacga
     3601 ccacgtcctg cgagtcgcca tgggctcgcc gggcgaggcc cagatccgcg tctgggacga
     3661 cgtcgccaag cagttcgagg cggccaaccc tggcaccaag gtcgagatga actaccagga
     3721 cgacgacctg taccagacga tcggcctgcc gaacctgctc aacgggcgca acgccccgga
     3781 catctacttc gagtggacgg gcaatcgcct cgagcagcgg gtgaaggacg gcttcggcgc
     3841 cgacatcagc gaggcgttca agacggggcc gctggccggg ctgttcgacg acgcgaccct
     3901 cgcccagtac aaggtcgacg acaaggtcgt gatggtcccg tactcggccg acgtcaccaa
     3961 cgtgctgtgg ttcaacaaga agctgctcgc cgacgccggc gtggagcccc cgacgacgtg
     4021 ggaggagctg ctcgcggcgt gcgacgcgct caacgccaag ggcatcaccc cgatcgcgtc
     4081 cggcaacaag gacctgtggg cggccggcaa ctggctgtcc cacatggtct cgcgggtcgt
     4141 cggcgaggac gcctacgcgg cggtcctgag cggcaccggc aagttcgaca cgcccgagtg
     4201 ggagaaggcg ttcggctaca tcaaggagct ggccgaccac aagtgcgtca acgacagcgc
     4261 caactcgatc gccgacgacg cgggggcgca gctgttcttc ggcggccagg ccgcgatgca
     4321 cccgatcggc tcctggctcg tcagctgggc gatcgacgag gctcccgacc tcgacttcga
     4381 ctacgtgaac ctgcccgcga tgccgggcgg gaccggcaat caggacagcg tcatcggtgt
     4441 ggcgaccggg tacgtcgtca atgccaagag cgccaagatc cccgaggcga cctcgttcat
     4501 ggcgctgctc aacaacgagg ccaacgtcca ggccctgctc gaggcggagg tcacgccgct
     4561 gaccaagtcg gcgtccgcag gcaccgacat tgacagccgg agcgcgcggc tgagcaccct
     4621 gctcaacgag gcgccggcga tcgtcctgcc gccggacacg gggtacgacc tcaagatggc
     4681 cgacgcgctg tacgccgccg aagccgccgt cctgggcggg caacagaccc cgaaggaggc
     4741 gctggcggcg atcgacgcca agctcggccg ctagctccga gcccctcgcc ggtcgcccgc
     4801 acgccgtggg cgaccggcat ccttgccgcg catgaagcgc cgcccatgaa gccgacccgg
     4861 gccgtcaccc cctacctgtt cctggcaccg gccgtggccg tgttcggctt cgccgtcctc
     4921 gtcccgatgc tgctcaccat cggctacagc ctgaccgagt ggaacggctt cgggccgatg
     4981 acgttcgtgg ggctcgacaa ctacctggcc gcggcgtccg acgccgtgtt tcgcgactcc
     5041 ttcgtccacg tcctcggcta catcgcggcg acgctgctgc tcgaggtcgg cctcgggctc
     5101 gggctcgccg ggctcgtcag catccgccgc ggcggcctgt ggttccgggt cgcgatcttc
     5161 gcgccggtga tgctgccgat ggtcgtcgtc gcggtcctgt ggtcgttcgt ctacaacgcc
     5221 gacttcggac tgattaacgc gggcctcggg gcgatcgggc tcgaggggct ccagcgagtg
     5281 tggctgggcg acacctcgac ggcgctgctc gcggtcagcg tcgtgtcggg ctgggtgtac
     5341 gccgggttct acatggtgat cttctacgcc gcgttcagcc agatcccgtc cgaggtgctc
     5401 gaggcggccc ggatggacgg tgcgggcgag tgggccctgt tccggcgcat caaggtgccg
     5461 atgatccgca acgtcgtcgc cgtggccatt ctgctgtgcg tcaccggcgg gtttcagggc
     5521 ttcgacctgt tcttcgtgct caccaacggc ggtccctacg gcgcgaccga gatcccgacc
     5581 acctacctcg tcaaggtggt cttccgcaac ggcgacgtgg gctacgggtc ggcgatggcg
     5641 gtggtgctca ccgtcgtcgt gctcgccgtg ggcctcgcct acgcccggct ccaccgccgc
     5701 gggtcgacct cgtgaggcgg cgcagcctgc ccgacctgct ggtcctggcc ggcatggtcc
     5761 tgctggccgc cgtgttcttc ctcccgatgg tctggatggt catctcgagc ttcaagtcga
     5821 acagcgagat cttccagtcg ccgttcgccc tgccgaccca ggtcgacctc gggcgctggg
     5881 cggaggcgtg ggaggtgggc cacctcggcc agtacgcgct caacagcgcg atcacgacgg
     5941 ccgtgtcggt attcctgatc ctcgtgctcg gggcgagcgc cgcgttcgcg ttcagccgct
     6001 accggttccg tggtcgtggc ttcctgctca gcctgttcgc gctcgggctc atcctgccgc
     6061 tgcagtcgta cttcatcgcc cagtcgacgc tgttcaacca gctcgcgatc acggacacac
     6121 ggctggcgct gatcatcccg tacgccgcga tgggcctctc gctcgccgtc tacctgctca
     6181 aggtctacct cgacgcgctg cccgacgagc tgttcgatgc cgcgcgcatc gatggcgcgg
     6241 gcgacgcccg ggtgttctgg ctgctcgccc tgccgctgct caggccgggc ctcgccacgg
     6301 tcgcgatctt ctccgccctg gcggcctgga acgagttcct gctcgccctg ctgtacatcc
     6361 aggacgacgc gctcaagacg atcccgacgg gcctgctcgc attctccagc cgctacgtca
     6421 ccgactactc gctgctgttc tcggcgctga cgatcgtgac gctgccgatg atcctgatct
     6481 atatcgtgtt caaccggcac atcgtcgagg gcatcaccgc gggcagcgtc aagtaggcac
     6541 gtacggcgca acggatcgtg ccgactcgcg tccgaatgct cacgggggac agttcgttaa
     6601 tgcggcatcg atagggtgat gcctcgaccc cgacccggcg acccacatgg agggaccgat
     6661 gagcgagagc gagaccgaga acagccagcc cgtccgcacg cgccgggcga tcctcggagc
     6721 ggccgcggcg ggggccgtcg ggctgtcggt cgggcgcttc gtgcggcccg agacggcctc
     6781 agcggcaacc ggcgccatgc agtacggcgc cgacaacaac gcgggcgacg cgggcacgat
     6841 cctgcggtcg acgtacgagt tccaggcgct cgaggtccac gggaccgggg cgaacgccat
     6901 cgccggcctc ggcgagggcg cggagtcgac aggcgtcatt ggcgtagggt ccggccccgg
     6961 gttcggcgtc cgcggtgtgt ctaaccatgg cgtcggcctc cacggcgagt ccgtcgatgg
     7021 atacgcgctc aaggcatggg gcagggcggg cttctccacg gcggggacgg ccacggtcgc
     7081 gcgacacaag gcgtccgtga aggtgaagcg cgacgaggtc tcgccgggct cgctggtgat
     7141 cgccacgatc cagcgcgacc gcgcggacac gtacgtgcgc ggggtcgtcg tcgacgacgg
     7201 ctcgttccgg gtcctgctca accgggcggt tgcgtcgccg accaaggtcg cgtacatcgt
     7261 gatcgagaag ctggagccgt tccccgactg acgatcgcgg ccgtcaggcc gcgagggccg
     7321 atgacgcctc gggcggtcca tcatcgacgg gctcggcgcg atccaggtcg aggccgcgga
     7381 gcgagggcac cgcgagcgcc acgaggccga cgacaccgag cgtgagcacg ccgcccccga
     7441 cgacgctcgg gacggttcca aacgcgtgcg cggcgaatcc gctctcgaac gcaccgagct
     7501 cgttcgatgc gccgatgaag atccagttca ccgaggcgac gcggccccgg atccgctccg
     7561 gtgacagcac gcgcaggatc gtctggcgga tcaccatgct gagcccgtcc gtgaccccgc
     7621 tcacgaacag ggcgagcacg gacagcgcga acgtcgtcga caggccgaac acgatcatgc
     7681 tcgcgccgaa cccggcgacg cagatgagca gcgtccgacc cgcgtggcgg ctgggcggcc
     7741 ggcgcgtcgc gatgagcatc acgcccagtg ccccgatgga cggcgcggtc cgcatgatcc
     7801 cgaggccgat cgggccgacg tgcaggacgt cggtcgcgaa caccggcagc agcgcgatcg
     7861 cgccgccgaa gaacacggcg aacaggtcga gggccatcga gccgagcagg gcaggcgtcc
     7921 ggcgcacgta gcggatcccg tcgagcaggc tttgcaggcg cggctcgccg gcgacgatct
     7981 cgggcatcgg ccggcgcggg atcagggcca ggcagaccgt cgagatcgcg agcaggaccg
     8041 ccatggccgc ataggtgccc gcgattccga tgacgaccag cgcgatgccg cccgtgagcg
     8101 gcccgagaat gccgccggcc tggctgacgc tgctcgtgta cgacacgccc tggaccgcct
     8161 gcacgcgcgg gatgacctgg gcctcgaagg cggtcaacgc cggtcgctcg aagccgctcc
     8221 cgatgcccgt cacgaacacc accgcgagga tcggaaggat ccccgaggcg ccgtcggcgg
     8281 ccagcagtcc gagcgccgcc gcacagccgg tcacgagggc gctcgtgatg acgatgatcc
     8341 ggcgccggtc gtgccggtcc gcgacataac cgccgaacag caccaacgac agcgccggaa
     8401 tggcctcgac gagcccgagc caaccgagcg cgagcgggtc gccggtcatg gcgtagacct
     8461 ggaacccgac gacggtcacg agcgcgctca tcgccaggct gttggtgaac cgcgcaccga
     8521 gcagcgcccg gaagtcgcgc tgcttcatga gcggcgggat ctccaccggg gcggcggcga
     8581 ggccgggcgg cggctggtcg gggtgactgg tcgcgctggt cgtgtccaag gagctccgag
     8641 gatagccccg gccgacccgc gtgcacccat cggtcgtccc atcgcaccgg cgccatgaat
     8701 cgcctcacgg atgccccgcc cgtcgcagca gggcggtcgc ggcgaaacgg gtggcaccac
     8761 gcatgcaccg tccgtcgcgc gcatgcaacc ggacgaccgg ccgatgcaca agttctggtg
     8821 gccggcggcc cgtgaggccg ggccgccggc gcacgatcag acgctcggcg tggccgtgtc
     8881 gtccggtgtg gtcggcgtgg tcggcgacgc gccggagcca cccgagccgg cggagtcatc
     8941 gcccatgttc gggcagtcct tgcccgcgtg gctcggacgg cccgacgggg ccgtcgtccc
     9001 gccgttcgac ggggcgccgg tgcccccgtc cgagggctgg gtgggggtcg taggggtcgt
     9061 ggacggcgcc gggctggccg acggatccgc ggccagggcg accgcggtgc cgccgagggc
     9121 gaacaggccc acggtcagca ggacggatgc gatggcgcga cggccgccgt tcaggcggga
     9181 cggccccgcg tcgggcgccg ctggctgggt ggtcggttgc gtgaactcgg tcatcgagtg
     9241 ctccgtgacg aggagtgcga acgggatagc tgaacgcgtc gatcgatagg cggccctctg
     9301 acgggttgga ctgctcgatc gtcgtcagcc gcaggtgaga gcccgctgag gaccgggcgt
     9361 ggctcgggaa cttcgcagcc ggctcccagc caactgccag ccgcgaacac ggatgttccc
     9421 gggcgccgtg agccgccata ctggacaccc tcgggaggat cgagctacgg aggagcgggg
     9481 atgattcgga atctcttgtc aggcatcgcc agtggcctgc tggtgtgggc gtacctgctg
     9541 atctgggacg cgaccgactt cacgacccca ttcacggcct atttctttgc cggcgtcgtc
     9601 gcggcgcttg gcggatggat ctggccggtc gtcatcggct ggttcctggt ccgtcgtcac
     9661 aggagcaagc aggacgagca gatccagaag gaagtcgacc gccagatcgc cgagaagagc
     9721 ggccagtagc gctcgcagcg gcacgccatt ggggccaccc gacgggggtc cgccggcccg
     9781 actggggtga gggcggcccg gtgatgtggg gaggctcgtg ctcccacgca ccggaggcgc
     9841 cgttgaccgc ccgtctgttt atccccacac ccctgcgact gatcgccatc ctgctcacgc
     9901 tggtggtcgt cgcgacgctc gaagcgtccc tcgccggtcc cgccctcggc gcaacacagc
     9961 ccctgtcgcg gggcaagctc gtggagacct cgggctgcac cgagatcctg atctcggcca
    10021 acgtcatcaa gtaggacgtg cctagcgccg gccgttgcgt cgccgcgcgc ggtaccaggc
    10081 gagatactcg atgagctcct cccgctcgtc gtcgctcagg tcggcgaaca gcgccgcgct
    10141 gacggcgcgc gcatcgcccg gctctgcgtc ccccggcacg cggtagccac acagccgcat
    10201 gagctctgca tacgggagtc cgagggcctc gctcagctgg tggaggacgt gcggcgacgg
    10261 gcgcttgacg gcatcgctct cgagcttgct gaggtacgcg ggcgaaatgc ccgcggcgcg
    10321 agccgcgtcc acgccggaca gctcacgcac gtcgcgtgcc tgcttcagca cggatccgat
    10381 cgactgcgcc acggcctgca ttctcctaca gtagcgtcgt gccactacag ttgacaatat
    10441 cacttgcgcg tggttcactg ccatccctag cgcaaggtcg ttttcgatgc cgctgccgct
    10501 gttgatcatc gccgtcgccg ccatgacggg cctcggcgtc ctgcgagtgg tccgcgtcca
    10561 ccttgaccgg acaccgctcc ccgagggccg agcgcggacc ctgttcaaga tcgccttcct
    10621 cgtcgtcccg ccggtcgtcc tgggcgttcc cgccggtgcc ccgctcggtg gcctcgcgtg
    10681 ggtcccgctc tacgccgtga tcctcggcgg gctgctggtg ctggtgacca tcgccgccgc
    10741 gatcgtcgag cgcgtcgcaa agggccggac gcggcaggtg ctgatgctcg cgctggtcgg
    10801 cagcgagggc gatccggagg acctcccgtt cgacccgccg gtgacggcga ggctcgccga
    10861 gagcatggcg atcgtcgacc gggcgaacgc ccagtttccg cgcgggctgg cgttcccgag
    10921 ccaggtcgat cgggctgact tccggtcggc ttggggcacg ctcgacgatg cgactcgtgt
    10981 cctcgagggc cacatcgtcg aggaccgaag actcggcctg gggacggcgg ctgccgcgtc
    11041 ggtgaccgcc gctgacgccc gtggtcgcct cgacaccctc gtgagcattg ccctccgccg
    11101 cggcctcgcg gccccggcct gatccagatg gctgaactga cgccacaccc cgtcgcgcag
    11161 atggcgcggc ggatccccgc ggccgctcgt gcgacgcgga ccggggcgca gagcacgacc
    11221 agcgccctgc agcgactccc cgactcgtcg ctgcgctggc tggctgctgc ctcgatcggg
    11281 ctcgctgccg gactgcagat ctccggcgcg ccacggttga cgcgatgggt gggcatggct
    11341 cccgcgctcg tcatcggtgc ggccctggcg ctacgcccac cggaaccggt cgcgaccgcg
    11401 gccgtcctgg agctggttcc ggcccatgca ccgggcgagg tgcggtcgtc gtgaccccac
    11461 tgcagaacca ggcgatcacg accttgccca acgtcatccg ggatcagacc atgccgaaca
    11521 cccttggcgg cgtgagcgat ccgagtccat tcctgccacg gcgcgcgccg tccgtcgcgt
    11581 cagcggttgt cgcggccggg ctcgtggcga cggtcatcgc ggccgtgctg gcgattggag
    11641 gtcgcttcgc gtaatcgacg cggcgacgtc agccgccctt gagcctgcgc cagccaccgg
    11701 cccggttgtt ggcgatgatc tggcggaaca tcgcgctcaa cgctggttcg ttgatcgcct
    11761 cgccacggcg gaaggccacc gtgcgggccg tcgtgttgtc gtggcccgcc gtgacgatgc
    11821 cctcgggatc ggggacgatc ccgccgtcgt agagaaacac gttgacgtgg tccttggcgg
    11881 ccagcagcgc gcagatgttt ccgttgagga cgaagtacgg ctgcacggtc cgcttgatcg
    11941 tctccgccac ctcggggtcc gcgtcgtgga cgaggtcccg gacccggtgg cagatctcct
    12001 gctgccagtc gggcagggcg tcgatgtagg cgtcgaccct ggggtcccgg acgtaggcca
    12061 tggcagccgc ctcctccctg ccagcgtagt ccacgcgctc gaacggccac gcctcgacga
    12121 gccaggaccg gacggcgtgg tagaggaccg gatcgaggtg ggcgaggtcc gcgcgcaccc
    12181 acgaacggca ctgggcgtcg aacccggcgg cctccgccgg atagatgtac acgcagccga
    12241 tgacgacgtc ggcgtcgggc tcgagcacgg tgtaggtgaa cccgcggcgg gccgcgaagt
    12301 cgtcggcatg ccgaacgagg tcgccgaagt tctccgcccg cgtcatcgga tgaggccagc
    12361 caagcccctc gaatcccggt gtgcgccgga tgtggtcgac gccggcggtc caggccgcgt
    12421 agtcggactc gttgtgttcc gggcctagcg ggcagaggcg gaagtccgcc gcatcgaggc
    12481 ccgtcggcgg cacgaactcg tccgggacga agggggccat gcggctagca gccggcggcg
    12541 tccattgctc ccgcgatcgc gccctggtgg cccaggaacg cagggatcgt tccgacgttg
    12601 gtgttgtagg tgtcgacagc gtcctgggtg accgggtccg tcgcgaacag caccttcgcc
    12661 gcctcgccgg ccttctcata ggcctcgatc gccgtcttcg ggtcgccgag gaccgattgc
    12721 tgcccgtcga gcgcatgcag gtccttgagc gcggccagga actcgtcgag atcgaggttg
    12781 ccgaccgtcc cggacttcac ggcctcgaca ttggccgggt cgcgaagctg ggcgagcagc
    12841 tgcactgcga cgatgagctt ggtggctgcc gccttgacct tgacgcagtc gacgctgccg
    12901 ttgcccgcag gcgcggcggt gtcggccggc gcctcagcgt tcgtggccgc cggcttcgtg
    12961 gtggacgcgg ttccgccgcc gccgcacgcg gcgacgaggg cgatcaacag gatcggggtg
    13021 acgagcttga tgcgcatacc cgcatcctat cgccgtcgat ttgccccgta cccggtacgt
    13081 tcgggcaatg cgactcgcgt cagggtcggc tgcgtcggcg ggccagctgc gcgtagaagg
    13141 cggtcgtgcc gatcaggccg gcgacgaatc cggcgagcgc gacgggactc cccatgccac
    13201 ccgtcgcgag gccggcgttg gtcgccacga gcccggcgcc ccccaggagg gcacttgccg
    13261 ccgcccgcct cgacctccgg tccggccagg cagacagccc gacgaggatg agccaggtgc
    13321 cgatgagcga ctggccgctc atgatgggca tcagggactc ctcgaacgtc atccgacggc
    13381 tgatgagcag cgtggtgaag acggtgatga cggcaacgcc gacgacgtcg caagccgccc
    13441 ctaggctcag cgcgcgcgag ggtggctcgc gccgggtgag ctggacggcg atcggcaccg
    13501 ttgcccatgc cagggcgatc gagaggctgt cgttgatggt cccccacggc tcgccggccg
    13561 cgaagaacac cccgagggcg atcgctgccc cgatggtcag gccggcggcc gcccccgaag
    13621 cacgccgacc agcgacgtcg agatcaagcc cgcagcaaga actcgcatcc gcgagcgaaa
    13681 cggtggtcac ggtgtcctcc tgtcggcgat gggacggtgc atcagaacgg cttgacttcc
    13741 atcagccagg tgatcacgac gatgcccgcg atcgcgatcg agcccccgat cgtcgggcga
    13801 gggtccgaca gcagccgctc cagtgcggcg tcatcgacgg gccttgggac cacgccggcc
    13861 ttcgtgtcgg cctggctcgg catccccagg ccccggcgca cgccgcccat ggggatcgac
    13921 aggagcgggg tcatgagcgc cacgatcacg atcaacacgc cgatcgacgc ccacagccac
    13981 cactgaccgt tgaaccacca gccagcggcg atcccgacca ggatcccgct gacgatcagc
    14041 agcaggccgg cgagcatcat cggggtgatg aaggcggcgg acaggtcgag gagcgacgcg
    14101 agcttggtcc ggtcgcgctc gcggcggacg cgccaccaga cgcccatcga gacgccgtgc
    14161 acggccagga aggcgaaggc actcaggacg tggagcacca gcagccacgg atacaggcca
    14221 gcccaggtca tcggacggtc tcctcagtgc accgagatcg cgccgaccat gccggcgtcc
    14281 tgatggcccg gcacggcgca gtagaacggg agctgtcccg cccccacggg cttgacggcc
    14341 acgaaggtcg tcgaatccgc cgggagcggg acgtggatcc cgagcgcgtc gacgtcgaac
    14401 gagtgcgcga acccgtcctt gttggtgacg aagacgccga gcacgtcgcc tgaccttgcc
    14461 tcgagcccgg tctcgaggaa cttcgtgtcc cgcgccgtca gggcgacggt ggtcgccggc
    14521 ggctgatcga gggcaacccc gacgggcgcc gccgacgcgg cgacgacgga ggtcacgcag
    14581 gcaccggcga ccaggccggc caggccggcg gcgacgaagc cggcgcggcc ccgctgctcc
    14641 cacagcggac gctgacggcg aacctcgagc caggccgtca gacttccgac caccaccagc
    14701 gccccggcga ccaggaccac cagcgtggtg acgaaggtca gggcgtcggc cggatgcgcc
    14761 agcgcgtacg gaccgtagaa gccgttgaac agcagcacga gaatgcccag cgcgctcgac
    14821 accagccaga accacggtcg cgttcgcgcg ccggaccacg cgacggaggc gagacccaca
    14881 cacccgaaca ggacgatgaa cccggcgatc accgcgagga agtccgcggc gaacggctgg
    14941 gtcgcgtaga ccacgaagac cgcgatgatg gccgcgtacg agatcccgac cagacgcatt
    15001 gcgaacgtac gcacgtgccg cctccacgtc ctcgccccgc cgagttggca ccggcgatcc
    15061 aggcgaggtc agtggcggga tgctatattg gttgcatcat caagtcaata gagttgaggt
    15121 ccccgcttgc ccaggaagta cgacctcgga agacgcgcgg aacagcaggc cgacacacgg
    15181 cgccggatca tcgacgcggc catggagctg taccaggagc agggcgcctc ggggacgacg
    15241 atgctcgacg tggcccggcg tgccgacgtg gccccgggaa cggtcgccaa ccacttcggc
    15301 tccgccgctg ccctggcggc cgtcgtcacg gaccaggtcc tgagcgacct gcacgtgccg
    15361 acgcccgacc tgttcgacgg tgtcgaggac ctgtccgacc ggatcgaggt gctcgtccgg
    15421 gagctggccg ccttcatgga ccgcagtgag acctggtgga aggtcaccca gcgcgagccg
    15481 gccggcgccc aggtctgggc cgacgcccag gcgcggtact acgcggagct cgataccctc
    15541 gtccgggcgg cgctcggacc gcttgccgcc gatgccgacg cggtcgcggt ggtgactacg
    15601 atcctgggca cctgggtgat cggcgcgatc caggcgacgg gccggagcgc cgagacggcc
    15661 gtcgcgctcg tgtccgacct gctggcgacc tggctcgcga cgcgaacgac gtaggcgacg
    15721 gtcaggcgcg cccgacgtcc acgacggggt tgcgcagcag gccgatgccc tcgatcgaga
    15781 tcgacacctc gtccccggcg gtcaggctga ac
//
LOCUS       DBNHXO010000155         7243 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2816854, whole genome shotgun sequence.
ACCESSION   DBNHXO010000155 DBNHXO010000000
VERSION     DBNHXO010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 7243)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7243)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7243
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2816854"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..504)
                     /locus_tag="AC162A_03290"
     CDS             complement(46..504)
                     /locus_tag="AC162A_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR5009891.1"
                     /translation="MATLIEIAEGRFAIPLLDDDGIRDLLRSGPRIAVVGASANPIRP
                     SLGVMRALRRAGYDVVAVSPRETEIDGLPCYPSLTAAVAATGRVDVVDVFRRSDQCAA
                     HAREAVAAGARCLWLQLGIANAEAARIAHDAGLSVVMDRCTIVEHRRLAA"
     gene            723..1865
                     /locus_tag="AC162A_03295"
     CDS             723..1865
                     /locus_tag="AC162A_03295"
                     /inference="COORDINATES: protein motif:HMM:NF014647.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR5009892.1"
                     /translation="MQVTKRLLALGAASAILITACSGTGATTAPATPAAAKSCVAFDT
                     GGLGDKGFNDLAKKGLDDAAALGFATQSAEAKNATDYEGNIQRLLDAGCKTIITVGFL
                     QGSATAKATLANPTIAFGQVDATWNSAGDDFTPGTADDPPPGVPANFTGLDYQVDQAA
                     MLAGYLAASWSKSGKVATFGGLAFPGVTRFMDGFYAGVKYHNEKKGTNVEVVGWDGSL
                     EQQARIDAGTFVGGTGSTDTWNNPSKGEELAKIFLDQGVDIVHPVAGETGNGTIKAML
                     AAGKWAIGVDNDQAITIPSSAGAILTSAQKAIDVSVLDVIKKTSGGDLGGEDYSGTLA
                     NNGVLLSPFHEFDTQISAETKSEIDALKAAIIDGSVKVCTYLGGGC"
     gene            1958..3484
                     /locus_tag="AC162A_03300"
     CDS             1958..3484
                     /locus_tag="AC162A_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5009893.1"
                     /translation="MRLELRDITKRFPGVLANDAISISADRGKVLGLLGENGAGKTTL
                     MNILSGLYRPDSGQIFIDGEERTFDGPAAAIHAGVGMVHQHFMLVPVFDVVEAVALGA
                     ETVTNRVGTFNRAAARKRIVELSDQYRLDVDPDAKIENLPVGVRQRVEILKALYRHSD
                     ILVLDEPSAVLTPYETEELFGIIRSLTATGTTIVFITHKLNEILEVADSVVVLRRGRV
                     AGTADPKTATRQDLANMMVGRDVELIVSKGPAKPADVVLSLRGVQVRDDRLELAVNGV
                     DLEVRAGEIVVIAGVQGNGQTELIEAIAGLRPFDTGEATIAGEHIEKTTPRTVSDLGV
                     AHIPEDRGRDGLIATMTIAENYVLDTYHREPYSRRGILDRKAVDAGAAKGVKEFDIRT
                     PSIETMAGSLSGGNQQKVVVAREFSVPVKLVLAAQPTRGLDVGSIEYIHRRLIEQRDA
                     GAAILLVSTELDEVIAVGDRIAVMFEGRIVGIVSGEDATHENLGLLMGGAEFGGGVAA
                     "
     gene            3481..4692
                     /locus_tag="AC162A_03305"
     CDS             3481..4692
                     /locus_tag="AC162A_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009894.1"
                     /translation="MTRTRELLRRALVPLLAIITALLVGAIVIVLTDFENLSRLGTDP
                     LGAIGGAFGGVVGAYGSLLAGAFGDPARIVAAFETGNPKDIATAIRPLTESLVAATPL
                     ILTGLAVAVSFRAGMFNIGIDGQLMIGALGATITAFALQGQVPGFAILLLSIAVGVLS
                     GGFWGFIPGFLKARTGAHEVITTIMLTYIASQIIFFALRSPILRAPGSTAPVSKPMSN
                     FVDIPLLINLPAIRLDFGFLIALLMAGVVSFLLFRTTKGFELRASGFNMTAARYAGMS
                     AGGSMMLAMAISGALAGLAGSFLVIGTVGQLSLDLSGGVGYTAIALALLAGLRPLGVV
                     LAALLFGALTSGGLTMGIQSGISAELLGFIMALVILFVAAPNLIRSMWRIKVVDPAKE
                     AVAPHQPTSAV"
     gene            4689..5990
                     /locus_tag="AC162A_03310"
     CDS             4689..5990
                     /locus_tag="AC162A_03310"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009895.1"
                     /translation="MSATTNVAVTASGGLAAQRATRNSRIRAVLLFAFGAFAFYLGSR
                     SLGTPATFSFWVNEQGGESVKLETVVGVLWIVAGLVSWFVAILQLLRGAPFPWRPWLL
                     LLIWPWVAAVLAEVLAGKPANLTGMIGGSLEFAVPITLGALAGILSERSGMFNIALEG
                     KMLVGACVAAVAASVTNLATGNALLAVLVGIAAAMVVSGFLGLLLAWMGIRHRMDQII
                     AGTVINIGAVGITNFIFLRILSRNTQFNTPPTIEPWRIPIFSDIPILGPIFFAAKPYV
                     YLMYVMVAVLTYMLFRTRWGLRLRASGEKPAAAGTVGIDVIRIRYQSLFLAGLIAGLA
                     GSYLSLSVAGTFGMEMVAGKGFIALAAVIFGAWHPVGALGAALVFGFAGTTQTLLSIL
                     GVDVPPQLLNSVPYLVTIIVVAGVVGRVRGPAAAGQPYEQG"
     gene            5993..7039
                     /locus_tag="AC162A_03315"
     CDS             5993..7039
                     /locus_tag="AC162A_03315"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR5009896.1"
                     /translation="MSAGYGDELGAVRARPTFYGVAAAPRTAPVGTSDHPLPLIIDSD
                     PGLDDALAIGLALARPELDVLAVTTVGGNADVTHCTENALRLLHAYGRDDIPVAEGAA
                     GALLGSIVRATEVHGESGIGSTRLEPATAAVHADHAVDLMARILADHPEPVAIAPIGP
                     LTNIALLLRLHPDLAPRIAHLCLMGGSIGEGNSTVSAEFNIYADPEAADVVFRSGVPI
                     TMMGLDVTHQALLDRSHAGALRDTGTRSGRIAAELTDFALDRNMQWSGSAVTAIHDAV
                     AIAHLATPDLVEVAPYHVTLDTTNGPARGRTVCDGQPYRLRRDGRAPNADVGIVIDGE
                     RFATILIDAFASLP"
     gene            7036..>7243
                     /locus_tag="AC162A_03320"
     CDS             7036..>7243
                     /locus_tag="AC162A_03320"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR5009897.1"
                     /translation="MTAPIPVLLDCDPGHDDALAITLALARPELDVLGITTVAGNVGL
                     RETTRNTLRLLALLGRTDVPVASGA"
BASE COUNT         1069 a         2592 c         2454 g         1128 t
ORIGIN      
        1 cctccggggg aacggaccgg gccagtatgc ccgggcgacc ggcgctcagg ccgcgagccg
       61 gcggtgctcg acgatcgtgc accggtccat cacgaccgac aggcccgcgt cgtgcgcgat
      121 ccgcgccgcc tcggcgttgg cgatcccgag ctgcagccac aggcaccgtg cccctgccgc
      181 gaccgcctcg cgcgcgtgcg cggcgcattg gtcggagcgg cggaagacgt ccacgacgtc
      241 gacacgtccg gtcgcggcga cggcagcggt caggctcggg taacacggca ggccgtcgat
      301 ctcggtctcg cgcgggctca ccgccaccac gtcgtacccg gcgcggcgca gggcgcgcat
      361 cacgcccagc gacggccgga tggggttcgc ggaagccccg acgaccgcga tccggggccc
      421 cgaccggagc aggtcgcgga tgccatcgtc atcgagcaag gggatcgcga accggccctc
      481 ggcgatctcg atcagcgtcg ccacacgctc ctgatccgcg tcccgaaccg tcatcgcaaa
      541 cgagcccctg tcaatgcccg gcgacgccgt cctgcatggt cgcgagccga gctcttctga
      601 accagcggaa tcgtgcccga agattgtggt gcaggcatcg cgcacgaccg attcttcgcg
      661 tactctacgg ctcgacccgg ccctcagccg aggggtgctc gggatcaatg gaggagtgca
      721 cgttgcaggt cacaaagcgg ctgctcgcgc tcggcgcggc gtcagccatc ctgatcaccg
      781 cctgttcggg caccggggcc acgaccgccc cggccacgcc tgccgcggcc aagtcctgcg
      841 tcgcgttcga cacgggtggg cttggcgaca agggcttcaa cgacctcgcc aagaagggcc
      901 tcgacgacgc ggcagcgctc ggatttgcga cccagtctgc cgaggcgaag aacgccacgg
      961 actacgaggg caacatccag cgcctgctcg acgcgggttg caagaccatc atcacggtcg
     1021 gcttcctgca gggatcggcg acggccaagg cgacgctcgc caacccgacc atcgcgttcg
     1081 gacaggtcga cgccacgtgg aactcggcgg gtgacgactt cacccccggc accgcggacg
     1141 atccgcctcc tggcgttccc gcgaacttca cgggcctcga ctaccaggtc gaccaggccg
     1201 cgatgctcgc cggctacctg gcggcgagct ggagcaagtc cggcaaggtc gcgacattcg
     1261 gcggcctggc attcccgggc gtcacccggt tcatggacgg cttctacgcc ggcgtcaagt
     1321 accacaacga gaagaagggc accaacgtcg aggttgtcgg ctgggacggc tcgctggagc
     1381 agcaggcgag gatcgacgcg ggcaccttcg tcggcggcac cggcagcacc gatacctgga
     1441 acaacccgtc caagggcgag gagctcgcca agatcttcct cgaccagggc gtcgacatcg
     1501 tccatccggt cgccggcgag accggcaacg ggaccatcaa ggcgatgctg gcggccggca
     1561 agtgggcgat cggcgtcgac aacgaccagg cgatcaccat cccgtcgtcg gcaggtgcca
     1621 tcctgacctc ggcgcagaag gccatcgacg tctccgtcct cgacgtcatc aagaagacct
     1681 cgggtggtga cctcggcggc gaggactact cgggcacgct cgccaacaac ggcgtcctcc
     1741 tgtcgccgtt ccacgagttc gacacccaga tctcggccga gaccaagtcc gagatcgacg
     1801 cgctgaaggc tgcgatcatt gacggcagcg tcaaggtctg cacctacctc ggcggcggct
     1861 gctagtcagg cgcgtgcgac cgtagagcca caacggccgc ccccggcccg ccacgcgggc
     1921 cgggggcggt ctcgcactac cctcgagggc ccagctgatg cggctcgaac tgcgcgacat
     1981 caccaagcgg ttccccggcg tcctcgccaa cgacgcgatc tcgatctcgg ctgaccgggg
     2041 caaggtcctg ggcctgctgg gcgagaacgg cgccggcaag acgacgctca tgaacatcct
     2101 gtccgggctg tatcggccgg actcgggtca gatcttcatc gacggcgaag agcggacgtt
     2161 cgatggtccc gccgcggcga tccacgccgg cgtcgggatg gtccatcagc acttcatgct
     2221 cgtcccggtg ttcgacgtcg tggaggccgt ggcgctcggc gccgagacgg tcacgaatcg
     2281 cgttgggacg ttcaatcggg ccgcggcccg gaagcggatc gtcgagctgt cggatcagta
     2341 caggctcgat gtcgaccccg acgccaagat cgagaacctc cccgtcggcg tccgccagcg
     2401 ggtggagatc ctcaaggcgc tctaccggca cagcgacatc ctcgtgctgg atgagcccag
     2461 cgccgtgctc acgccgtacg agaccgagga gctgttcggg atcatccggt ccctgacggc
     2521 aaccggcacg acgatcgtct tcatcacgca caagctcaat gagatcctcg aggtcgccga
     2581 cagcgtcgtc gtcctccgcc gtgggcgcgt cgccgggacg gcagacccca agaccgcgac
     2641 ccgccaggac ctggccaaca tgatggtcgg ccgcgacgtg gagctgatcg tgtccaaggg
     2701 cccggcgaag cccgcggacg tggtgctcag cctgcgcggc gtgcaggttc gcgacgaccg
     2761 cctggagctg gccgtcaacg gcgtcgacct cgaggtccgt gccggcgaga tcgtggtgat
     2821 cgccggcgta caggggaacg gccagaccga gctgatcgag gcgattgccg gtctccgccc
     2881 gttcgacacg ggcgaggcga cgatcgcggg cgagcacatc gagaagacca cgccacgcac
     2941 ggtgtccgac ctcggtgtgg cccacattcc cgaagaccgc ggacgcgatg gcctgatcgc
     3001 cacgatgacg atcgccgaga actatgtcct cgacacctat caccgcgagc cgtacagccg
     3061 gcgcggaatc ctcgaccgca aggccgtcga cgcgggagcg gcgaagggcg tcaaggagtt
     3121 cgacatccgc acgccgtcga tcgagacgat ggcggggtcg ctgtcgggtg gcaaccagca
     3181 gaaggttgtc gtcgcgcgcg agttctcggt cccggtgaag ctcgtgctgg cggcccagcc
     3241 gacgcgtggc ctggacgtcg gctccatcga gtacatccat cgccgactca tcgagcagcg
     3301 cgatgccgga gcggcgatcc tgctcgtgag caccgagctc gacgaggtga tcgcggtcgg
     3361 cgacaggatc gccgtgatgt tcgagggcag gatcgtcggc atcgtgtccg gcgaggacgc
     3421 gacgcacgag aacctgggcc tgctgatggg tggcgccgag ttcggcgggg gcgtcgcggc
     3481 atgaccagaa ccagggagct cctgcgcagg gccctcgttc cgctgctggc aatcatcacc
     3541 gccctcctgg ttggcgcgat cgtgatcgtg ctcacggact tcgagaacct gtcgcggctc
     3601 gggacggatc cgctcggcgc gatcggcgga gcttttggcg gtgtcgtggg ggcctacgga
     3661 tcgctgctcg ccggcgcgtt cggcgatccc gcccgcatcg tggccgcgtt cgagacgggc
     3721 aacccgaagg acatcgcgac cgcgatccgc cccctgaccg agtcgctggt cgcggcgacc
     3781 ccgctgatcc tcaccggtct cgccgtcgcg gtgtcgttcc gcgccggcat gttcaacatc
     3841 ggcatcgacg gccagctgat gatcggcgcc ctgggcgcga ccatcacggc cttcgccctc
     3901 caggggcagg tgcctgggtt tgcgatcctc ctgctctcga tcgcggtcgg cgtgctgtcg
     3961 ggtgggttct gggggttcat tccgggtttc ctcaaggcgc gcacgggcgc ccacgaggtc
     4021 atcacgacga tcatgctcac ctacatcgcg tcgcagatca tcttctttgc tctgcgctcg
     4081 ccgatccttc gggcgccggg cagcaccgcg cccgtctcga agccgatgtc gaacttcgtc
     4141 gacattccgc tgctcatcaa cctgccggcg atccggctcg acttcgggtt cctgatcgcg
     4201 ctcctgatgg ccggggtggt ctccttcctc ctgttccgga cgacgaaggg cttcgagctc
     4261 cgggcgtccg gattcaacat gacggccgcg cgctatgccg gcatgagcgc cggcgggtcg
     4321 atgatgctgg ccatggcgat ctcgggcgcg ctggccggcc tggcgggctc gttcctggtc
     4381 atcggcaccg tgggccagct gtcgctcgac ctgtccggcg gggtcgggta cacggcgatc
     4441 gcgctcgcgc tgctggccgg tctccggccg ctgggcgtgg tcctcgccgc gctgctgttc
     4501 ggagccctga cgtcgggcgg cctgacgatg ggcatccagt ccgggatctc ggccgagctg
     4561 ctcgggttca tcatggcgct ggtgatcctg ttcgtcgccg caccgaacct catccggtcg
     4621 atgtggcgga tcaaggtcgt tgacccggcc aaggaggcgg tcgctcccca ccagccgacg
     4681 tcggccgtat gagtgccacg accaacgtcg cggtgaccgc cagcggcggt ctcgcggccc
     4741 aacgcgcgac ccggaactcg cggatccgcg ccgtcctcct gttcgcgttc ggcgcgttcg
     4801 cgttctacct cgggagccgc agcctcggga cgcccgccac cttctcgttc tgggtgaacg
     4861 agcaaggcgg cgagagcgtg aagctcgaga cggtcgtcgg cgtcctgtgg atcgtcgccg
     4921 gtctcgtctc gtggttcgtc gccatcctcc agctgttgcg gggtgcgccc ttcccctggc
     4981 gcccctggct cctgctcctc atctggccgt gggtcgcggc cgtgctcgcg gaggtgctcg
     5041 cgggcaagcc ggcgaacctg accgggatga tcggcggcag cctcgaattc gccgtcccga
     5101 tcacgctggg agccctcgcc ggcatcctgt ccgagcgcag cggcatgttc aacatcgccc
     5161 tcgaaggcaa gatgctcgtg ggcgcctgcg tcgccgcggt cgccgcgagc gtcaccaacc
     5221 tcgccacggg caatgcgttg ctcgccgtgc tggtcggcat cgccgccgcc atggtcgtct
     5281 ccgggtttct cgggctcctg ctcgcgtgga tgggcatccg ccaccggatg gaccagatca
     5341 tcgcgggcac cgtgatcaac atcggtgcgg tggggatcac caacttcatc ttcctgcgga
     5401 tcctgagccg gaacacgcag ttcaacacgc caccgaccat cgagccgtgg cgcattccga
     5461 tcttctccga catcccgatc ctcggcccga tcttcttcgc cgcgaagccc tacgtgtacc
     5521 tgatgtacgt gatggtcgcg gtgctcacct acatgctgtt ccggacgcgc tggggcctcc
     5581 gcctgcgggc gtccggcgag aagcccgccg cggccggcac cgtgggcatc gacgtcatca
     5641 ggatccgcta tcagtcgctg ttcctggcgg ggctgatcgc cggcctcgcg ggctcctacc
     5701 tgtcgctctc ggtggccggc accttcggga tggagatggt ggccggcaag ggcttcatcg
     5761 ccctcgccgc ggtgatcttc ggcgcgtggc atccggtcgg cgccctcggt gccgcgctgg
     5821 tgttcggctt cgctggcaca acgcagacgc tgctgtcgat cctcggcgtc gacgtcccgc
     5881 cccagctcct caacagcgtc ccgtacctgg tcacgatcat cgtcgtcgcc ggcgtcgtcg
     5941 ggcgggtgcg cgggccggcg gccgccggcc agccctacga acagggctaa tcgtgtcggc
     6001 gggttacggc gacgagctgg gtgcggtccg cgcccggccc accttctacg gcgtcgccgc
     6061 ggcgccacgc acggcgccag tgggcaccag tgaccacccg ctgccgctga tcatcgacag
     6121 cgaccccggg ctggatgacg cgctggcgat cggcctcgcg ctcgcacgcc ccgagctcga
     6181 cgtcctcgcg gtgaccaccg tcggcggcaa cgcggacgtc acccattgca cggagaacgc
     6241 gctccggctg ctgcacgcct acggccgcga cgacatcccg gtcgccgagg gcgccgccgg
     6301 agcgctcctg ggctcgatcg ttcgggcgac ggaggtccac ggcgagagcg gcataggttc
     6361 gacgcgcctg gagcccgcga cagcggccgt ccacgcggac cacgcagtgg acctgatggc
     6421 caggatcctc gcggaccacc cggagccggt cgcgatcgcg cccatcgggc cgctgacgaa
     6481 catcgcgctg ctcctgcggc tgcacccgga cctcgcgccg aggatcgcgc acctgtgcct
     6541 gatgggtggc tcgatcggcg agggaaacag caccgtgtcc gccgagttca acatctacgc
     6601 cgaccccgag gccgccgacg tcgtgttccg ctcgggcgtg ccgatcacca tgatgggtct
     6661 cgacgtgacc caccaggcgc tgctcgaccg gtcccacgcc ggggcgctgc gggacaccgg
     6721 aacgcgctcg ggccggatcg ccgccgagct cacggacttc gcgctcgacc ggaacatgca
     6781 gtggtccggg tcggcagtga cggccatcca cgatgccgtg gcgatcgccc acctggcgac
     6841 ccccgacctc gtcgaggtcg cgccgtacca cgtcaccctg gacacgacca acgggcccgc
     6901 ccgcggccgc acggtctgcg acggccagcc gtaccggctc cggcgtgacg gccgggcgcc
     6961 caacgcggac gtcggcatcg tgatcgacgg cgagcgcttc gcgacgatcc tcatcgacgc
     7021 cttcgcgagc ctcccgtgac tgcccccatc ccggtcctgc tcgactgcga cccgggccac
     7081 gacgacgccc tggcgatcac gctcgccctc gcgcgaccgg agctggacgt cctggggatc
     7141 acgacggtgg ccggcaacgt gggcctgcgc gagaccaccc gcaacacgct gcggctgctc
     7201 gcgctgctcg gccgcacgga cgtgccggtc gcctcgggcg cgg
//
LOCUS       DBNHXO010000156         1703 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_435877, whole genome shotgun sequence.
ACCESSION   DBNHXO010000156 DBNHXO010000000
VERSION     DBNHXO010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_435877"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="AC162A_03325"
     CDS             complement(<1..393)
                     /locus_tag="AC162A_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012447.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HIR5009898.1"
                     /translation="MTEAHFVPERAAGSHDRVVLTGADLTIADVEAVARRDARVALDI
                     HARDRMQEARDVIETLVARGEVVYGVTTGFGDLATTFIPPGDAAALQVNLITSHAAGV
                     GPAFPREVVRAMLLLRANTLALGHSGCRP"
     gene            429..1097
                     /locus_tag="AC162A_03330"
     CDS             429..1097
                     /locus_tag="AC162A_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR5009899.1"
                     /translation="MLTFIDQVVIPFLTSLYSAVGYLGLAAAMAIESAMIPLPSELIL
                     PFAGFLVSDPTQVEPITGQPWNFWIAVIVATIGNTLGSLVAYAIGAYGGRPFLERYGR
                     YLLIRPHEIEAADEFFRKYGAATVFFGRLLPIVRTFISFPAGVTRMPIGKFIIYSTAG
                     ALPWSIALVYAGMVLGANWVEIREILRPFDVAIFIAVIVVFGLLLWWRMGMPGLRRKA
                     TPAE"
     gene            complement(1108..>1703)
                     /locus_tag="AC162A_03335"
     CDS             complement(1108..>1703)
                     /locus_tag="AC162A_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR5009900.1"
                     /translation="ALARRLAPVAFDRVLTSPLFRARETAAIVAPGSSIEADPRLREM
                     DYGAWEGRTYDQITEADGAFRGEWERAPDVLACPGGESGNDVAERVRSFLGDLLDEHR
                     AWHAKASFRAATSGWGSTTPKIERPILAVAHSTTNRVLICVALAIEIREFRLRLDQDQ
                     ANLTVLQWALDGAPDQGRMLLMNDTAHLRRANEVPWA"
BASE COUNT          221 a          607 c          601 g          274 t
ORIGIN      
        1 cggccggcag ccggagtgac cgagcgcgag cgtgttggcg cgcaggagca gcatcgcgcg
       61 cacgacctcg cgcgggaacg ccggcccgac gcccgccgcg tgcgacgtga tcaggttgac
      121 ctggagcgcc gccgcgtcac cgggtgggat gaatgtcgtc gcgaggtccc cgaagccggt
      181 ggtcacgccg tacacgacct cgccccgcgc gaccagcgtc tcgatgacgt cgcgcgcctc
      241 ctgcattcga tcccgcgcgt ggatgtcgag ggcgacccgg gcgtcgcgtc gggcgacggc
      301 ctcgacgtcg gcgatcgtca ggtcggcgcc ggtgagcacc acgcggtcgt ggctaccggc
      361 tgcgcgctcg ggaacgaaat gggcctccgt caccgtgccg aggatagtca gatatcctcc
      421 cctgcgttgt gctcacgttc atcgaccagg tcgtcatccc gttcctgacc agcctctact
      481 ccgccgtggg atacctcggc ctggcggccg cgatggcgat cgagtcggcg atgatcccgc
      541 tgccgtccga gctgatcctc cccttcgcgg gcttcctcgt gtcggacccg acccaggtcg
      601 agccgatcac cgggcagccc tggaacttct ggatcgcggt gatcgtggcc acgatcggca
      661 acacgctcgg gtcgctggtc gcgtacgcga tcggcgcgta cggtggccgc cccttcctcg
      721 agcgctacgg ccgctacctg ctgatccggc cacacgagat cgaggccgcg gacgagttct
      781 tccgcaagta cggggccgcg acggtcttct tcggccggct gctgccgatc gtccgcacgt
      841 tcatctcgtt cccggccggc gtcacccgga tgccgatcgg caagttcatc atctactcga
      901 ccgccggcgc gctgccgtgg tcgatcgccc tcgtctacgc cggcatggtg ctcggcgcga
      961 actgggtgga gatccgcgag atcctgcggc cgttcgacgt cgcgatcttc atcgcggtga
     1021 tcgtggtctt cgggctgctg ctgtggtggc ggatgggcat gcccggcctg cggcgcaagg
     1081 cgaccccggc cgagtagcgg ctcgtcccta cgcccagggg acctcgttcg cgcggcgcag
     1141 gtgcgcggtg tcgttcatca gcagcatgcg gccctggtcg ggcgcgccgt cgagggccca
     1201 ttgcaggacg gtgaggttcg cctggtcctg gtccaggcga agcctgaact cccggatctc
     1261 gatcgccagc gccacgcaga tcaggacccg gttggtcgtg gagtgcgcga cggcaaggat
     1321 cggccgctcg atcttcggcg tggtcgagcc ccagcccgac gtcgccgccc ggaaggacgc
     1381 cttcgcgtgc caggcccggt gctcgtcgag caggtcgcca aggaagctgc ggacgcgctc
     1441 ggcgacatcg ttgccggact cgccgcccgg acacgccagc acgtcggggg cgcgctccca
     1501 ctcgccccgg aacgcgccgt cggcctcggt gatctggtcg taggtccggc cctcccaggc
     1561 gccgtagtcc atctcgcgca ggcggggatc cgcctcgatc gatgacccgg gcgcgacgat
     1621 cgcggccgtc tcgcgcgcgc ggaacagggg gctcgtcaac acccggtcga aggcgacggg
     1681 cgcgagccgg cgggcgagtg cgt
//
LOCUS       DBNHXO010000157         3173 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2816957, whole genome shotgun sequence.
ACCESSION   DBNHXO010000157 DBNHXO010000000
VERSION     DBNHXO010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2816957"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="AC162A_03340"
     CDS             <1..848
                     /locus_tag="AC162A_03340"
                     /inference="COORDINATES: protein motif:HMM:NF015644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HIR5009901.1"
                     /translation="TQPVTFFNNTDRWTVPTEQTNAKSLAPEAYYVVMRMPGEAKAEF
                     LLLQPMIPRSRPNMIAWVAARNDAPNYGTVRAYQFPADTTIFGPSQIEARIDQDPIIS
                     AQVSLWDQSGSEVIRGNLIVVPVGDSLLYLQPVYLQSTSSAFPEFQRIVVASPTTVVW
                     ARTLSGALNLLLTTQAGGTPGASPSPGPSPGPTPAPTATPAPGTTPGPTVAPTAAPTT
                     PPDGALPTDVSGLIDYANTHFDLAQAALRNGDFATYGTEMARVQAALEALGRLTESPA
                     PSLAP"
     gene            845..1855
                     /locus_tag="AC162A_03345"
     CDS             845..1855
                     /locus_tag="AC162A_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009902.1"
                     /translation="MTTAAVAPSAAAPDRADRLPIIAVLSGAFVVTLVRPVSWALGLA
                     GFLAGGGLALMAWPVLVLPTPTGIQNALGGPVSTLTVGTPSTAMLVLIWGGLICALVL
                     FVGALLAGAWAERNVIAVGLEAAGDEGMLAPRDLRGAPGTGRVAILRLFALLPVIAVA
                     VAAWQPLYDAAYHELVLPDELATPLGLRIVGDVPWLVAALLATWVAADAAGALGVRRL
                     VIERRPVLVAWGLGWVDILRSPLRVIATAAFGLGVIALLAIPAMLAAASGWARVREVM
                     AQSTDPLPAIGTVVTWVAIWLGGLVLAGVGAAIRAAAWTLEATPVEPSGEGIRREGGE
                     PA"
     gene            1995..2633
                     /locus_tag="AC162A_03350"
     CDS             1995..2633
                     /locus_tag="AC162A_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR5009903.1"
                     /translation="MPDEIINTINDAIGGIVNNPIVQFAMQALVVYFVILWLAAAYWA
                     FRDMQQRSENPVLPYLAAALIVLFTPLLFIFGIIVYRIIRPHEKIGEVYERNLAEEAL
                     LAEVEAIKTCPSCKRRVNDEWIICPTCRTQLSRVCANCGRLVGLDWALCAWCGRDFER
                     SDLAAYEPLATQLPGRLQPTQPPAGLAQSPRTGRVRQAPPTSRGAPGTIPEP"
     gene            2630..>3173
                     /locus_tag="AC162A_03355"
     CDS             2630..>3173
                     /locus_tag="AC162A_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009904.1"
                     /translation="MTEPAAPDPEGTAPRPSTFSLEGRAVPAIYLIGWVGSVMGLAVI
                     LVSFMASGSSAAGWLFMGGLIVLAIGLVAAGGSQAIESSRRTDLAYRGPSPVLAFLAV
                     FAITLIATVLVLAPLSAMGFDVASPAGTTISLLITMVAYIAIVRLLVVGTGALSWAEM
                     GVHRPTTESVRDLAIGAVFAV"
BASE COUNT          382 a         1208 c         1098 g          485 t
ORIGIN      
        1 tcacgcagcc ggtgacgttc ttcaacaaca cggatcgctg gacggtgccg acggagcaga
       61 ccaacgcgaa gagcctcgcg ccggaggcgt actacgtcgt gatgcgcatg ccgggcgagg
      121 ccaaggcgga gttcctgctc ctgcagccga tgatcccccg cagccgtccg aacatgatcg
      181 cgtgggtggc cgcccgcaac gacgctccca actacggcac ggtgcgtgcg taccagttcc
      241 cggccgacac cacgatcttc gggccgtccc agatcgaggc acggatcgac caggacccga
      301 tcatcagcgc gcaggtctcg ctctgggacc agagcggcag cgaggtcatc cgcggcaacc
      361 tgatcgtggt gccggtgggc gactcgctgc tgtatctcca gccggtctac ctgcaatcca
      421 cgtcgtcggc gttccccgag ttccagcgga tcgtcgtcgc gagcccgacg acggtcgttt
      481 gggcccggac gctgtcgggc gcgctgaacc tgttgctcac cacccaggca ggtggaacgc
      541 cgggagcctc gccgtcgccc ggaccctcgc ccggaccgac gccggccccc accgcgaccc
      601 cggcgccggg gacgacgccc ggcccgacgg tggcgccgac cgccgctccc acgacgcccc
      661 ccgacggcgc gttgcccacc gacgtgtccg ggctcatcga ctacgcgaac acgcatttcg
      721 acctcgccca ggcggcgctg cgcaacggcg acttcgccac ctacgggacc gagatggccc
      781 gcgttcaggc cgcgctcgag gccctcgggc gcctgacgga gtcgcccgcg cccagcctcg
      841 cgccttgacc acggcggccg tcgcgccgag cgccgctgcg cccgatcgcg ccgaccgcct
      901 gccgatcatc gccgtcctgt caggggcgtt cgtcgtgacg ctcgtccggc ccgtgtcttg
      961 ggcgctcggg ctggccgggt tcctcgccgg cggcgggctc gcgctgatgg cgtggccggt
     1021 gctggtgctg ccgacgccga ccgggatcca gaacgccctc ggtgggccgg tgagcacgct
     1081 gacggtcggc acgccgtcga ccgcgatgct cgtcctgatc tggggcggcc tcatctgcgc
     1141 tctcgtgctc ttcgtaggag cgctgctggc aggggcgtgg gcggagcgca acgtgatcgc
     1201 ggttgggctc gaggcggccg gcgacgaagg catgctcgcc ccgcgcgacc tccggggagc
     1261 gccggggacc gggcgcgtcg cgatccttcg gctgttcgcc ctgttgccgg tcatcgccgt
     1321 cgcggtcgcc gcatggcagc cgctctatga cgccgcctac cacgagctgg tcctgcccga
     1381 cgagctcgcg acgccgctgg ggctgcgcat cgtcggcgac gtgccctggc tcgtcgcggc
     1441 gctgctggca acctgggtcg ccgcggacgc cgcgggcgcg ctgggtgtcc gccggctggt
     1501 catcgaacgg cgcccggtcc tcgtcgcgtg gggcctcggc tgggtcgaca tcctgcgcag
     1561 cccgctgcgg gtcatcgcca ccgccgcgtt cgggctcggg gtcatcgcgt tgctggcgat
     1621 ccccgcgatg ctcgcggcgg catccggctg ggcccgggtg cgcgaggtca tggcccagtc
     1681 caccgatcca ctccctgcca tcggcaccgt ggtgacatgg gtcgcgatct ggctgggcgg
     1741 gctggtcctc gccggcgtgg gtgccgcgat ccgcgccgcc gcctggacgc tcgaggcgac
     1801 acccgtcgag ccgagcggcg aaggcatccg gcgggagggc ggcgaacccg cctgacgggg
     1861 tccgcccggg cgtcacccac ggcctccccg gagcgtcgac tccgtgctcc ggcgaggccg
     1921 ggtgctcctg ctagactgct ccacgccgcg cgcccccggg cgcgccctgc cccgatggag
     1981 gctgcacccc ccgcatgccc gacgagatca tcaacacgat caacgacgcg atcggcggga
     2041 tcgtcaacaa cccgatcgtg cagttcgcga tgcaggcgct cgtcgtctac ttcgtgatcc
     2101 tgtggctcgc cgcggcctac tgggccttcc gggacatgca gcagcggagc gagaacccgg
     2161 tcctgccgta cctcgcagcg gcgctgatcg tgctgttcac cccgctcctg ttcatcttcg
     2221 ggatcatcgt ctaccggatc atccggcccc acgagaagat cggcgaggtc tacgagcgca
     2281 acctggccga ggaggcgctg ctcgccgagg tcgaggcgat caagacctgc ccctcgtgca
     2341 agcgccgggt caacgacgag tggatcatct gccccacctg ccgcacgcag ctcagccgcg
     2401 tatgcgccaa ctgcggacgc ctggtcgggc tcgactgggc cctgtgcgcc tggtgcgggc
     2461 gcgacttcga acgctccgac ctcgcggcct acgagccgct cgcgacccag ctgcccggcc
     2521 gcctccagcc gacccagccg ccggccggcc tggcgcagag tccccggacc gggcgcgtcc
     2581 gccaggcgcc gcccacgagc cgcggcgcac cgggcacgat cccggagcct tgaccgagcc
     2641 ggccgcgccg gacccggagg ggaccgcccc gcgcccgtcg accttctccc tcgagggacg
     2701 cgccgtcccg gccatctatc tgatcggctg ggtcggctcc gtgatggggc tggccgtcat
     2761 cctcgtctcg ttcatggcct caggctcgtc ggcggccggc tggctgttca tgggcggcct
     2821 catcgtgctc gcgatcggcc tcgtcgcggc cggcggctcc caggccatcg agagcagccg
     2881 gcgtaccgac ctcgcctatc gcgggccgtc gcccgtgctc gcgttcctcg ccgtgttcgc
     2941 gatcacgctg atcgccaccg tcctcgtgct cgcgccgctg tctgcgatgg gcttcgacgt
     3001 cgcctcgccc gcgggcacca cgatctccct gctgatcacg atggtggcct acatcgcgat
     3061 cgtccggctg ctggtcgtcg gcaccggcgc gctgtcgtgg gccgagatgg gggtgcaccg
     3121 tccgaccacg gagtcggttc gggatctcgc gatcggcgcg gtgttcgctg tcc
//
LOCUS       DBNHXO010000158         5819 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1824798, whole genome shotgun sequence.
ACCESSION   DBNHXO010000158 DBNHXO010000000
VERSION     DBNHXO010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1824798"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..841)
                     /locus_tag="AC162A_03360"
     CDS             complement(170..841)
                     /locus_tag="AC162A_03360"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR5009905.1"
                     /translation="MTRSTLSFDSAVATGRGDDGTTGLLFGGDRVRKDDARTEAYGTV
                     DEAVAAIGLARAELGLKAQYGVLSPAFGGLAELLLRLQRELFAVGAELATNPDNRGRQ
                     QDGATRVSREMVDGLDTVLRETEAKIELPREFVVPGETRVSAALEIARTVVRRAERRT
                     VTLADADEAIARGLVLPYLNRLADLLWILARVAEQAEARQSTVARPSRARGRSNAASP
                     ERSES"
     gene            complement(855..1208)
                     /locus_tag="AC162A_03365"
     CDS             complement(855..1208)
                     /locus_tag="AC162A_03365"
                     /inference="COORDINATES: protein motif:HMM:NF013999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIR5009906.1"
                     /translation="MTDTQPVTETAATSTAAPEVVPYDPQRRATELAILDALRAVVDP
                     EIGMNVVELALIKQIILGPDSCEIKMILTTPFCPYAGAMIAQVTEQAESVVDYPVKVT
                     LLAERWDPRDAGLMW"
     gene            1284..1772
                     /locus_tag="AC162A_03370"
     CDS             1284..1772
                     /locus_tag="AC162A_03370"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR5009907.1"
                     /translation="MGTKTAVVRLADDELRFEATVGSGHTIVLDDAARDSGARPSELL
                     GAALAACTGMDVISILRKKRQPVTGYEIRVIGHQMSTQPHAYTRFDVVHVVDGVGVDP
                     EAVRRSIELSATKYCSVGSTLAAGTLEIHHSYLLRDADGSERTGDVVVMGPNEPYERA
                     TG"
     gene            complement(1769..2095)
                     /locus_tag="AC162A_03375"
     CDS             complement(1769..2095)
                     /locus_tag="AC162A_03375"
                     /inference="COORDINATES: protein motif:HMM:NF016167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR5009908.1"
                     /translation="MTIKWVKVDPLVMNGEPFCYGTRLTVRQVLELRANGYTVVDLLK
                     DHPELRLVGIAEAYTYAAANRDRYADFFAVDGSLEGPGLTAEEAAGLPDRLRVPGVVV
                     KVAAPA"
     gene            complement(2106..2588)
                     /locus_tag="AC162A_03380"
     CDS             complement(2106..2588)
                     /locus_tag="AC162A_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009909.1"
                     /translation="MTHPSLGLPPPDLTKGFPDAGARLRTNDLRLATRAVEVAATEDP
                     TYADRIGEAGLRNLLQDATVFVDKLALCVAGDDPRWLREFADQTATVYRRRGIQLDDV
                     TKLFEGIRASIRGVLTAEELVPADAALDEAIAVFKWHRRLAGDARKRNPILAALYKGG
                     "
     gene            2635..3222
                     /locus_tag="AC162A_03385"
     CDS             2635..3222
                     /locus_tag="AC162A_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009910.1"
                     /translation="MSDEQGEPRPEGQRMLDRAPGERYRSRPANAAASAAPVPLPVER
                     SMVLAIGVPMAVAGVGAALVAVLGSFDIGVGLLALSGFIGWAVGVAVVWSGVPDSDAR
                     GQRALWAVVLAVGSIVLGIVILWVWSHVEGGVMDPFAYLDARFGPIAYLNVIVAGVVA
                     YQRARRAGRRVRPALTPSAGRRMSRRPSPCESRAA"
     gene            complement(3146..4465)
                     /locus_tag="AC162A_03390"
     CDS             complement(3146..4465)
                     /locus_tag="AC162A_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HIR5009911.1"
                     /translation="MSRERVPAVGGTRDVPAPDAVARDYILLGLRLDQHLSGTVDGYF
                     GPAELKAKVDMEQLRSPARLAEDAAALRSRLEAEVRDPDRRHWLDLQLVALETLARVK
                     AGETIAYLDQVARCLTLHPERRPDARFEEAAARLEAALPGAGTLAERLAAEDAAWTVP
                     SERVSAVIDVLVSRFRDRAAARFGLPEGEALRVSLVRDQPWSGYNWYDGGFRSRVDLN
                     LDLPIRLPTLVATMAHETYPGHHLEHATKEQVLVEELGRLEASILLINTPECLISEGL
                     GNAGIAFAVPPDEMAGLLVELAPVAGLSMAQERDALLAAAARHSAVAEQRARLTETRI
                     NAALMLHVDGVSLDRAIAYQVEVGRANPDTAAKRVEFISHPLWRLYDYVYTEGEALLT
                     AWLDLVPPADREARFGRLLREQLTPPAIRTEMAAATSGDRPMESGRV"
     gene            complement(4462..5004)
                     /locus_tag="AC162A_03395"
     CDS             complement(4462..5004)
                     /locus_tag="AC162A_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009912.1"
                     /translation="MTRDELILRTRQLIAEGERLMAQPSLTALQTWLQLSDDLLATAW
                     GTMDRYHLSWLMVGKPKGIVRGREMTPEEEAAYVREVAEQKTAALRMSLDAVERQGMP
                     FRGETGGVGAGQGMGEVPAADGDVAAQAQAHIAGTTRRSIPLDPEMQARLDEARRVAA
                     DHATHDDGAPRAPRNDRLQR"
     gene            5056..5661
                     /locus_tag="AC162A_03400"
     CDS             5056..5661
                     /locus_tag="AC162A_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HIR5009913.1"
                     /translation="MADLTPHDIGWFESTDELRNWLDTHHATAREKWVGMRPKAAGLA
                     TVTWEQIVDEVLCVGWIDGIRKKADGGSVIRITPRRTGSVWSARNVGRVEALRAAGRM
                     RPAGEAAFGERREDRTAIYSFEGNLALDEAAEAAMRRDASGWAFWEAQPPGYRRLATH
                     WVMSAKRPETRARRLGQLIELCAAGRRLPQLTGSTQRPTTT"
BASE COUNT          790 a         2007 c         2203 g          819 t
ORIGIN      
        1 acctgctggt tgccgcccac gaggaaggcg acgccggccg tcagcagcag gccggcgaac
       61 atccagccga aggcgccgac cagcaactgc tggacgagcg ccggcgccgg ccggacgccg
      121 agctggctcg gggacagggg ttggctgctc ggggtctggg ggatgccgct cacgattcgc
      181 tcctctcggg tgacgccgcg ttcgatcggc cgcgagccct cgagggccgg gccacggtcg
      241 actgtctcgc ctcggcctgc tcggccacgc gggccaggat ccacagcagg tcggcgagac
      301 ggttcaggta gggcaggacg aggccccggg cgatcgcctc gtcggcgtcg gccagggtga
      361 cggtccgccg ctcggcgcgc cggacgaccg tccgcgcgat ctcgagcgcg gcggaaaccc
      421 gggtctcgcc gggcacgacg aactcgcggg gcagctcgat cttcgcctcg gtctcgcgca
      481 gcacggtgtc caggccatcg accatctcgc gggagacgcg cgtggcgccg tcctgttgcc
      541 gaccgcggtt gtccgggttc gttgcgagct cggccccgac ggcgaacagc tcgcgctgga
      601 gccgcagcag cagctcggcc aggccgccga aggccgggga gaggacgccg tactgggcct
      661 tcagcccgag ctcggcgcgg gcgagcccga tggccgcgac agcctcatcg accgtgccgt
      721 aggcctcggt gcgggcatcg tccttgcgca cgcggtcccc accgaacagg aggccggtgg
      781 tgccgtcgtc gccgcggccg gtcgcgacgg cgctgtcgaa cgagagcgtg gagcgcgtca
      841 tccgcgatcg gcgctcacca catcaggccc gcatcacgcg ggtcccagcg ctcggcgagc
      901 agcgtgacct tgaccgggta atcgacgacg ctctcggcct gctcggtcac ctgcgcgatc
      961 atcgcgccgg cgtacgggca gaacggcgtc gtgaggatca tcttgatctc gcacgagtcg
     1021 gggccgagga tgatctgctt gatcaacgcg agctcgacga cgttcatccc gatctcgggg
     1081 tctacgaccg cccgcagcgc gtccaggatc gcgagctcgg tcgcgcggcg ctgggggtcg
     1141 tacgggacga cctcgggtgc ggcggttgac gtggcggcgg tctcggtcac gggctgggtg
     1201 tcggtcatcg tatggaaatc ctagcaccac gctcggaaat gaccgtttgc gccccatccc
     1261 gatcggcccc acgatggagc gcgatgggca ccaagacggc ggtcgtccgc ctcgccgacg
     1321 acgaactgcg gttcgaggcg accgtcggca gcggccacac catcgtgctc gacgacgcgg
     1381 cgcgcgactc gggtgcgcgg ccctcggagc tgctcggggc ggcgctcgcg gcgtgcaccg
     1441 gcatggacgt gatctcgatc ctgcgcaaga agcgacagcc ggtcacgggg tacgagatcc
     1501 gggtgatcgg ccaccagatg tcgacgcagc cccacgccta cacgcggttc gacgtggtcc
     1561 acgtcgtcga cggcgtgggc gtggacccgg aggccgtccg gcggtcgatc gagctgtcgg
     1621 cgacgaagta ctgctcggtg ggctcgacgc tggcggccgg cacgctggag atccaccact
     1681 cgtacctgct gcgcgatgcc gacggcagcg agcgaacggg cgacgtcgtc gtgatggggc
     1741 cgaacgaacc gtacgagcga gcgaccggct aagcgggcgc ggcgaccttg accacgaccc
     1801 cgggcacgcg caggcggtcc ggcaaaccgg cggcctcctc ggcggtcagg ccggggccct
     1861 cgagcgagcc gtcgacggcg aagaagtccg catacctgtc gcggttggct gcggcgtacg
     1921 tgtacgcctc ggcgatcccg accaggcgca gctcgggatg gtccttgagc aggtcgacga
     1981 ccgtgtagcc gtttgcccgc agctcgagca cctggcgcac ggtcaggcgg gtgccgtagc
     2041 agaacggctc gccgttcatc acgagcggat cgaccttgac ccacttgatc gtcatcggtg
     2101 gtcgcctagc cgcccttgta cagcgcggcg aggatcgggt tgcgcttgcg ggcatcgccg
     2161 gccaggcgtc ggtgccactt gaacaccgcg atcgcctcgt ccagggcggc atcggcgggg
     2221 acgagctcct cggccgtcag gacgccacgg atgctggcgc ggatgccctc gaacagcttc
     2281 gtcacgtcgt cgagctggat gccccgccgg cggtacacgg tcgcggtctg gtcggcgaac
     2341 tcgcgcagcc agcgcggatc gtcgcctgcg acgcacaagg ccagcttgtc gacgaacacg
     2401 gtcgcgtcct ggagcaggtt gcgcaggccc gcctcgccga tccggtccgc gtacgtcggg
     2461 tcctcggtcg cggccacctc gacggcgcgc gtcgcgagcc gcaggtcgtt cgtccggagc
     2521 cgagcgcccg cgtccggaaa gcccttggtg aggtccggcg gggggaggcc gagcgagggg
     2581 tgggtcacgg tcggagagga taccgtcccg gcaatgggcg cgtacgatcc gagcatgagc
     2641 gacgagcagg gcgaaccccg gcccgagggg cagcggatgc tggaccgggc ccccggcgag
     2701 cgctatcggt cgcgtccggc gaacgcggcc gcctcggccg cccccgtccc cctcccggtc
     2761 gagcgatcga tggtccttgc gatcggcgtc ccgatggcgg tcgcgggcgt gggtgcggcg
     2821 ctggtcgccg tcctcggctc gttcgacatc ggggtgggac tcctcgcgct ctccggcttc
     2881 atcggctggg cggtcggcgt ggccgtggtc tggagcggcg tgccggactc ggacgctcgg
     2941 ggccagcggg ctctctgggc ggtcgtgctc gccgtgggtt cgatcgtcct cggcatcgtc
     3001 atcctgtggg tctggtcgca cgtcgagggc ggcgtgatgg acccgtttgc ctacctcgac
     3061 gcacgcttcg ggccgatcgc ctacctcaac gtgatcgtgg ctggggtcgt ggcgtaccag
     3121 cgagcgcgca gagccgggcg gcgagtcaga cccgccctga ctccatcggc cggtcgccgg
     3181 atgtcgcggc ggccatctcc gtgcgaatcg cgggcggcgt gagctgctcg cggagcaggc
     3241 gcccgaagcg cgcctcgcgg tccgccggcg gcacgagatc gagccaggcc gtcaacagcg
     3301 cctcgccctc cgtatagacg tagtcgtaca ggcgccacag cgggtgagag atgaactcga
     3361 cgcgcttggc ggcggtgtcc gggttcgcgc gcccgacctc gacctggtag gcgatcgccc
     3421 gatcgagtga cacgccgtcg acgtggagca tcagggccgc gttgatccgc gtctcggtga
     3481 gccgcgcacg ttgctcggcg accgccgagt gcctggcagc ggcggcgagc agggcgtccc
     3541 gttcctgggc catggacaac ccggcgaccg gtgccagctc gaccaggagg ccggccatct
     3601 cgtcgggcgg gacggcgaag gcgatcccgg cgttgccgag gccctccgag atcaggcatt
     3661 cgggcgtgtt gatgagcagg atcgacgcct cgaggcgccc gagctcctcg accaggacct
     3721 gttccttggt cgcgtgctcg aggtggtggc ccgggtaggt ctcgtgggcc atggtggcga
     3781 cgagggtcgg gagccggatc gggaggtcga ggttgaggtc gacgcgcgac cggaaaccgc
     3841 cgtcgtacca gttgtagccg gaccagggct ggtcgcggac gagcgagacc cgcagcgcct
     3901 cgccctcggg caggccgaag cgagcggcgg cgcgatcacg aaagcggctg acgaggacgt
     3961 cgatgacggc cgacacccgc tcggacggga ccgtccacgc ggcgtcctcg gcggcgaggc
     4021 gctcggcgag cgtgcccgcg ccggggagcg cggcctcgag gcgggcggca gcctcctcga
     4081 accgggcgtc aggacgccgt tccgggtgga gcgtcaggca ccgtgcgacc tggtccaggt
     4141 aagcgatggt ctcaccggcc ttgaccctgg ccagggtctc gagggccacc agttggaggt
     4201 cgagccagtg acgccgatcg gggtcgcgga cctcggcctc gaggcgggac cggagggccg
     4261 ccgcatcctc cgccagccgt gccggcgacc ggagctgctc catgtcgacc ttggccttga
     4321 gctcggccgg gccgaagtag ccgtcgacgg tcccggacag gtgctgatcg agccggaggc
     4381 cgagcaggat gtagtcgcgg gcgacggcgt ccggcgccgg gacgtcgcgg gtgccgccga
     4441 ccgccgggac gcgttcccgg ctcaacgctg gagccggtcg ttgcggggcg cgcgcggggc
     4501 gccgtcgtcg tgggtggcgt ggtccgcggc cacccgtcgc gcctcgtcga gccgtgcctg
     4561 catctcgggg tcgagcggga tcgagcggcg cgtcgtcccg gcgatgtgag cctgggcctg
     4621 cgccgcgacg tcgccatcgg ccgccggcac ctcgcccatc ccctgccctg cgccgacgcc
     4681 gccggtctcg ccgcggaacg gcatcccctg gcgctcgacg gcgtcgaggc tcatccgcag
     4741 cgccgcggtc ttctgctcgg cgacctcgcg gacgtatgcg gcttcttcct cgggtgtcat
     4801 ctcgcgcccg cgcacgatgc ccttcggctt gcccaccatc agccacgaga ggtggtagcg
     4861 gtccatcgtc ccccaggcgg tcgcgagcag gtcgtcggac agctggagcc acgtctggag
     4921 cgccgtgagc gacggctggg ccatcaggcg ctcgccctcg gcgatcagct ggcgggtgcg
     4981 caggatcagt tcgtcgcggg tcacgggttg gagtctagcc gcggggaccc ccccgaccga
     5041 cggtagcctt gggtgatggc cgacctcacg ccccacgaca tcgggtggtt cgagtccacc
     5101 gacgagctgc gcaattggct cgacacccac cacgcgaccg cgcgcgagaa gtgggtgggc
     5161 atgcgcccga aggcggccgg gctggcgaca gtgacctggg agcagatcgt cgacgaggtg
     5221 ctgtgcgtcg gctggatcga cgggatccgc aagaaggcgg acggcggctc ggtgatccgg
     5281 atcacgcccc ggcggaccgg cagcgtctgg agcgcccgga acgtcggccg cgtggaggcc
     5341 cttcgcgcgg cgggacggat gcggcccgcg ggcgaggcag cgttcggtga gcggcgcgag
     5401 gatcggacgg cgatctactc gttcgagggc aacctggcgc tcgacgaggc ggccgaggcg
     5461 gcgatgcgcc gagatgcctc cggctgggcg ttctgggagg cgcagccgcc cggctaccgg
     5521 cgcctcgcga cccactgggt gatgagcgcc aaacgtccgg agacacgcgc gcggcgtctg
     5581 ggccagctga tcgagctgtg cgctgccggc aggcggctcc cgcagctcac gggttcgacc
     5641 cagcgtccaa cgacgacctg agttcagccc tcgacgatga accgctcccg gtcagcgcgg
     5701 gcgcggaggg tccatggccc ggggacaccg tcgccgatcg gcgcgccgtc gaagcgcgtc
     5761 acgggcagca cgccggccac cgacgagcac acgaaggcct cgtcggctgc agccaggtc
//
LOCUS       DBNHXO010000159         4100 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4006786, whole genome shotgun sequence.
ACCESSION   DBNHXO010000159 DBNHXO010000000
VERSION     DBNHXO010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4006786"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="AC162A_03405"
     CDS             complement(<1..370)
                     /locus_tag="AC162A_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009914.1"
                     /translation="MEPPEAASTGGNAARRRPPPSRLSPQALAQTRADGAAEGLVWLG
                     REPEARASILYRFLRLVARFVIFGLFRFRISTSGRELLPASGGYLLIGAAHRGWMDPF
                     VVMHALPAQPRCWFLGSAPST"
     gene            complement(357..1433)
                     /gene="lgt"
                     /locus_tag="AC162A_03410"
     CDS             complement(357..1433)
                     /gene="lgt"
                     /locus_tag="AC162A_03410"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR5009915.1"
                     /translation="MPIPTALAALAPVLALIAITPDPIAFHLGPIPVAWYGICYAVGL
                     TLAYIVITREAKRRGLNARLVDNGIIIVAAAALIGGRLYHVIDQWALYKDDPIKIILP
                     PYAGLGVYGGIIAGTIAAWYITRRWHQSFAKWTDVIAPGLFVMQAVGRLGNFFNQELY
                     GPPTNLPWGIAIDCAHRVGDHGIAQYPCELYPEATTGFHPLFLYESISGILGAVTLLW
                     IARRYGRRMRPGDLFLIFLIWYSAVRFALETLRVGNWTFFGIPTAMVVSAVLIVGSLV
                     LLAIRHRPGAVFETWGDPPPPEEELLEGEEWIEDDEDDEADEADDLDEDADDDDDPDE
                     GDGDDEGTDDGSGRDAGPGGGGAA"
     gene            1527..1877
                     /locus_tag="AC162A_03415"
     CDS             1527..1877
                     /locus_tag="AC162A_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009916.1"
                     /translation="MSAEFNWWLLIVGVVAGVVLTWLVLADSNRREREVGEREIPAEA
                     AWISRSLGKPSVDEATAEQVLRAHRRYLGFPPPDVLVTADELYALQQERPASGDPEPG
                     EEPVGVNTRDDRPV"
     gene            complement(1874..2728)
                     /locus_tag="AC162A_03420"
     CDS             complement(1874..2728)
                     /locus_tag="AC162A_03420"
                     /inference="COORDINATES: protein motif:HMM:NF021604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor domain-containing protein"
                     /protein_id="HIR5009917.1"
                     /translation="MEHAEALEQIETAAVEPDGLERLMAGDTPGSAAVAGHLAGCPDC
                     AEELVRIRRTAIVARDVIRSEPDPALRDRTLAFVRSVGRDRSEGGAGASADRSTSITV
                     LPSPVQPPLAAETPQGTRPSRRGFALLAIAAAVVIAVGVGFAAGSAAQRQDVEGRQSE
                     IAVLEDATTTALRIQAQPDAQVVQLTATPSGGDATGSLAFSASTGELVAVATDLEPEE
                     ANEEYACWVEVEGQRQRLGQMYWAGDLWAWAGPVEGLEMLPEGAVFGVSLSPTDGGAG
                     DPVLTGSL"
     gene            complement(2728..3393)
                     /locus_tag="AC162A_03425"
     CDS             complement(2728..3393)
                     /locus_tag="AC162A_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5009918.1"
                     /translation="MTERQVGESEDATLVRAVANGSEDALAALYDRHVGGVHAVALRL
                     TSDRQVAEEVVQETFLALWNRAELFDPTMASLATWLRTIARNRTVDRLRAAGRRPPLV
                     SLGGRGDNDDFEGDVDRLGPAAVVLGGATADPGPEAAVEAADLRDAVVEALAGMPEVE
                     RLVIVLAYREGLTQAEIAERLEWPLGTVKTRTRRALARLRTALEGGHAPVGSSGGVPR
                     DGW"
     gene            complement(3398..3940)
                     /locus_tag="AC162A_03430"
     CDS             complement(3398..3940)
                     /locus_tag="AC162A_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013224.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR5009919.1"
                     /translation="MQLLYLTNRDLVPKNTPISPTDARLLALLQADGRRSYAELGNDL
                     GMAGPSAHERVKKLESRGVIRGYAADVDPVAVGLSVVAFTWVTQAPGTIASDLTPDFA
                     AIPEIEECHHIAGEADYILKIRARDMDHLGTIVRRVQTTDHVFSTETDVVFTTGFERR
                     PLPLGANGTTDPETGHDRSA"
BASE COUNT          594 a         1492 c         1436 g          578 t
ORIGIN      
        1 acgtcgacgg ggcgctgccc aggaaccagc agcggggctg cgcgggcagc gcatgcatca
       61 ccacgaacgg gtccatccag ccacggtgcg ccgccccgat caggagatag ccgcccgagg
      121 ccgggagcag ctcgcgcccc gaggtcgaga tccggaaccg gaacaggccg aagatcacga
      181 agcgcgccac cagccgcagg aaccggtaca ggatcgaggc ccgggcctcc ggctcgcgtc
      241 cgagccagac caggccctcg gcggcgccgt ccgcccgtgt ttgcgcgagg gcttgcgggg
      301 acagccgcga cgggggcggc cggcggcgcg cggcgtttcc ccccgtggac gccgcctcag
      361 gcggctccac cgcccccggg cccggcgtcg cgaccggagc cgtcgtcggt tccttcgtca
      421 tctccatcgc cctcgtcggg gtcgtcgtcg tcatcagcgt cttcgtcgag gtcgtcggcc
      481 tcgtcggcct cgtcgtcctc gtcgtcctcg atccactcct cgccctcgag gagctcttcc
      541 tccggcggcg gcgggtcgcc ccacgtctcg aacaccgctc ccggccggtg ccggatcgcg
      601 agcaggacga gggaaccgac gatcaggacc gccgacacca ccatcgccgt cgggatcccg
      661 aagaacgtcc agttgcccac ccgcagggtc tcgagcgcga accggacggc cgagtaccag
      721 atcaggaaga tcaggaacag gtcgcccggc cgcatccgtc gtccgtaccg acgcgcgatc
      781 cacagcagcg tgacggcgcc caggatgccg ctgatcgatt cgtacaggaa cagcgggtgg
      841 aagccggtcg tcgcctcggg atagagctcg cacggatact gggcgatccc gtggtcgccg
      901 acgcgatggg cgcagtcgat cgcgatgccc cagggcaggt tggtcggcgg cccgtacagc
      961 tcctggttga agaagttgcc cagccggccg accgcctgca tcacgaacag gccgggggcg
     1021 atcacgtccg tccacttcgc gaacgactgg tgccagcggc gggtgatgta ccaggccgcg
     1081 atggtgcccg cgatgatccc gccatagacc ccgagacccg cgtacggggg caggatgatc
     1141 ttgatcgggt cgtccttgta cagggcccac tggtcgatga cgtggtacag ccggccgccg
     1201 atcagcgcag cagcggcgac gatgatgatc ccgttgtcga cgagccgggc gttgaggccg
     1261 cgccgcttcg cctccctcgt gatcacgatg tacgcgaggg tcaggccgac cgcgtagcag
     1321 atgccatacc aggcgaccgg gatcgggccg aggtggaatg cgatcgggtc cggcgtgatc
     1381 gcgatcaggg cgagcacggg cgcgagcgcc gccagggccg tcgggatggg catggggcgc
     1441 agagtacagc ccggccgcga gtgtgcgtcg tctcggctat cctcggccgc gatgcacggc
     1501 cgtcccgaga cctcacgcgg cagccgatga gcgccgagtt caactggtgg ctcctgatcg
     1561 tcggcgtggt cgccggcgtg gtcctgacct ggctcgtcct ggcggactcg aatcgccgcg
     1621 agcgggaggt cggcgagcgc gagatccccg ccgaggccgc ctggatctcg agatcactcg
     1681 gcaagccctc ggtcgacgag gccaccgccg agcaggtgct gcgggcgcat cgccgctacc
     1741 tggggtttcc gccgcccgac gtgctcgtga cggcggacga gctgtacgcg ctccagcagg
     1801 agcggcccgc gagcggtgac cccgagcccg gcgaagagcc ggtcggcgtg aacacgcgag
     1861 acgaccggcc cgtctagagg ctccctgtca ggacggggtc gccggcgccg ccatcggtcg
     1921 ggctgagcga gacgccgaac acggcaccct cggggagcat ctcgaggcct tcgaccgggc
     1981 cggcccacgc ccacaggtcg ccggcccagt acatctggcc gagtcgctgg cgctgcccct
     2041 cgacctcgac ccagcacgcg tactcctcgt tggcttcctc gggctccagg tccgtggcca
     2101 ccgccacgag ctcgccggtc gaggccgaga acgcgagcga gcctgtggca tcgccgcccg
     2161 acggcgtggc ggtgagctgg acgacctgcg cgtcgggctg ggcctggata cgcagggcgg
     2221 tcgtcgtggc gtcctccagg accgcgatct cagactgccg gccctcgaca tcctgccgct
     2281 gggcggcgct ccccgccgcg aacccgacgc ccaccgcgat cacgaccgcc gccgcgatcg
     2341 cgaggagcgc gaagccacgc cgcgacggtc gcgtgccctg cggcgtctcg gcggcgagcg
     2401 gcggctggac cggcgacggc aacaccgtga tcgaggtcga acgatcggcc gatgcgcccg
     2461 ctccgccctc ggatcggtcc cgcccgaccg agcgcacgaa cgcgagcgtg cgatcgcgga
     2521 gcgccggatc gggctccgag cggatgacgt cgcgcgcaac gatcgcggtc cgccggatgc
     2581 gcacgagctc ctcggcacag tcggggcagc cggcgaggtg gccggccacg gccgccgacc
     2641 cgggcgtgtc gcccgccatc agccgctcga gcccatcggg ctcgactgcg gcggtctcga
     2701 tctgttccag cgcctcggcg tgttccatca ccatccatct cgcggcacac caccgctgct
     2761 cccgaccggc gcatggcccc cctcgagcgc ggtgcggagg cgagccaggg cccgcctcgt
     2821 gcgtgtcttc acggtaccga gcggccactc gagccgttcg gcgatctccg cctgcgtcag
     2881 cccctcgcga taggccagca cgatgaccag ccgctccacc tccggcatgc cggccagggc
     2941 ctcgaccacg gcgtcccgca ggtcggcggc ttcgaccgcg gcttcggggc cggggtccgc
     3001 cgtcgcacca ccgagcacca cggcggccgg gccgagccgg tcgacgtcgc cctcgaagtc
     3061 gtcgttgtcc cccctgccgc ccagcgacac cagcggcggt cggcggcccg ccgcccgcag
     3121 ccggtcgacg gtccggttgc gcgcgatcgt ccgcaaccag gtcgcgagcg acgccatggt
     3181 cggatcgaac agctccgcgc ggttccagag cgcgaggaac gtttcctgca cgacctcctc
     3241 ggcgacctga cggtcgcttg tgagccggag cgcaacggcg tggacgccgc cgacatgccg
     3301 gtcgtagagc gccgcgaggg catcttccga gccgttcgcc acggccctga cgagcgtggc
     3361 gtcctcggat tcgccgacct ggcgctccgt cataccctta cgcgctccgg tcgtgcccgg
     3421 tttcggggtc cgtggtcccg ttggcgccca agggcagggg acggcgctcg aagcccgtag
     3481 tgaacacgac gtcggtctca gtcgagaaca cgtgatcggt cgtctggacc cggcggacga
     3541 tcgtccccag gtggtccatg tcgcgtgccc ggatcttgag gatgtagtcg gcctcgccgg
     3601 cgatgtggtg gcactcctcg atctcgggga tcgccgcgaa gtcgggggtc aggtcgctcg
     3661 cgatcgtccc cggggcctgc gtcacccacg tgaatgcgac cacgctgagg cccacggcaa
     3721 ccgggtccac gtcggccgcg taaccccgga tcaccccccg cgactcgagc ttcttcacgc
     3781 gctcgtgcgc cgacggaccg gccatcccaa ggtcgttgcc gagctcggcg taagaacgcc
     3841 tcccgtcggc ctggagcagg gccaggagtc gcgcgtcggt cgggctaatc ggggtgttct
     3901 ttggcacgag atccctgttc gttaggtaca gcagttgcaa taccgatgat tattaggtag
     3961 actgccaccc gttagggtca ttgggacgtc gaccccgccg gatcggccgg cgggacacgg
     4021 gcaaccggtc cgcggcagtt ccaggcacag aaaggtgcac gacgatggtc atcgttgccg
     4081 cgttcgcgct cctcgcgctg
//
LOCUS       DBNHXO010000160         1680 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2022964, whole genome shotgun sequence.
ACCESSION   DBNHXO010000160 DBNHXO010000000
VERSION     DBNHXO010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2022964"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="AC162A_03435"
     CDS             <1..519
                     /locus_tag="AC162A_03435"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR5009920.1"
                     /translation="EASGVPFREGMVRNRYSGRTFIQPSQAMRQRGVNVKLSPLRETI
                     RDRRLIVVDDSIVRGTTTKQIVGLLRRAGAAEVHVRISAPPIYHPCFYGIDTSIETEL
                     IAATHSVEEIREFIGADSLGYLSIRGVLSALDLPYEQFCFACFDGNYPEPVPYDVTAR
                     KFLLETAGSGVG"
     gene            519..1655
                     /gene="purM"
                     /locus_tag="AC162A_03440"
     CDS             519..1655
                     /gene="purM"
                     /locus_tag="AC162A_03440"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR5009921.1"
                     /translation="MSERGTFTRVAYARAGVDVAAGDRAVELMRAAVESTRRPEVLGG
                     LGGFGSAFAIPEGISEPVIVSSTDGVGTKTAIAAALGRFDTIGIDLVGMCADDVVCSG
                     AEPVAFLDYIAVGRLDPRNVAELVAGVADGCRQAGAALVGGETAEHPGLMEAGEFDMA
                     GFCIGVAERSGLLDTTTARAGDAIVGLGASGLHANGYSLVRALVAQHDLDLRAPYQAL
                     LRRVLGDRTATTCLEREPEHGFATLGEVLLTPTRVYARDVLAIRAALAADGVGVRGIA
                     HITGGGLPGNVPRALPAGLGARLRPSAWPLPSVMRLFGVLGGIEDDELRATFNGGLGM
                     VLIGPDDAAERTVELAGERGIPAWVVGEVVDPGDLGDRRYGEAT"
BASE COUNT          210 a          601 c          630 g          239 t
ORIGIN      
        1 gaggcgtcgg gcgtgccgtt ccgcgagggc atggtccgca accggtacag cggccggacc
       61 ttcatccagc catcgcaggc gatgcgccag cgcggcgtca acgtgaagct cagcccgctg
      121 cgcgagacga tccgggaccg gcgcctgatc gtggtcgacg attcgatcgt gcgcggcacc
      181 accaccaagc agatcgtcgg cctgctgcgc cgtgccggcg ccgccgaggt ccacgtccgg
      241 atcagcgccc cgccgatcta ccacccgtgc ttctacggca tcgacacctc gatcgagacc
      301 gagctgatcg ccgcgaccca ctcggttgag gagatccgcg agttcatcgg cgccgattcg
      361 ctcggctacc tctcgatccg tggcgtgctc agcgctctcg acctgccgta cgagcagttc
      421 tgcttcgcgt gtttcgacgg caactacccg gagcccgtcc cgtacgacgt gaccgcccgc
      481 aagttcctgc tcgagacggc cggcagcggc gtcggctgat gagcgagcgg ggcaccttca
      541 cgcgggtggc ctacgcccgc gccggggtgg acgtcgccgc cggcgatcgt gccgtggagc
      601 tgatgcgcgc ggccgtcgag tcgacccggc ggcccgaggt cctcggcgga ctcggcgggt
      661 tcggatcggc gttcgcgatc cccgagggca tctctgagcc ggtgatcgtg tcctcgaccg
      721 atggggtcgg cacgaagacg gccattgcgg cagccctcgg ccggttcgac accatcggca
      781 tcgacctcgt cgggatgtgc gccgacgacg tcgtgtgctc gggcgccgag ccggtcgcgt
      841 tcctcgacta catcgccgtc gggcgcctcg acccgcgcaa cgtggccgag ctcgtggccg
      901 gcgtggcgga cggctgccgc caggcgggcg cggccctcgt gggcggggag acggccgagc
      961 acccgggcct gatggaggcc ggcgagttcg acatggcggg gttctgcatc ggggtcgcgg
     1021 agcggagcgg gctgctcgac acgacgaccg ccagggcggg cgacgcgatc gtcgggctcg
     1081 gcgcgagcgg tctgcatgcc aacggctatt cgctcgtacg cgcgctcgtc gcgcagcacg
     1141 acctggacct ccgggcgccc taccaggcgc tcctgcgccg ggtcctcggg gaccgaacgg
     1201 ccacgacctg cctcgagcgt gagccggagc acgggttcgc gaccctgggc gaggtgctgc
     1261 tcacgccgac ccgggtctat gcccgcgacg tgctggcgat ccgggccgcc ctggccgcgg
     1321 acggcgtcgg cgtgcgcggg atcgcccaca tcaccggcgg aggcttgccc ggcaacgtcc
     1381 cgcgcgcgct tcccgctggg ctcggcgcgc gcctccggcc gtcggcgtgg ccgctgccgt
     1441 cggtgatgcg cctgttcggc gtgctcggcg ggatcgagga cgacgagctc cgggccacct
     1501 tcaacggcgg gctgggcatg gtcctgatcg ggcccgacga cgccgccgag cgaaccgtcg
     1561 agctcgcggg cgagcgcggg atccccgcct gggtggtcgg ggaggtcgtg gacccggggg
     1621 acctcggcga ccggcgctac ggggaggcga cgtgagcaag gcgagcgcgc cgcggatcgc
//
LOCUS       DBNHXO010000161         1540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1230364, whole genome shotgun sequence.
ACCESSION   DBNHXO010000161 DBNHXO010000000
VERSION     DBNHXO010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1230364"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="AC162A_03445"
     CDS             <1..196
                     /locus_tag="AC162A_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018756002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HIR5009922.1"
                     /translation="LLVMFRVIANFSTTEVVLALPIAVQEMVLAVWLIAKGFAPLAIA
                     AEPAGETSRRPAGTSPVTVS"
     gene            201..665
                     /locus_tag="AC162A_03450"
     CDS             201..665
                     /locus_tag="AC162A_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HIR5009923.1"
                     /translation="MTTSTKPASMPPRWIVRAVWAVHRAIYSMTGGRRGLRNPTADRW
                     GMMRLRTVGRRSGQERTAILGYFEDGQDLVTMAMNGWTEGEPAWWLNLQARPDTVVEL
                     KDGPRVVRARAANAEERPRRWARWATYDKDLEGYAARRSGETAVVVLSPRPD"
     gene            778..1299
                     /locus_tag="AC162A_03455"
     CDS             778..1299
                     /locus_tag="AC162A_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009924.1"
                     /translation="MKRRLFAAAFASAISWVALAAPVSAHGDHDARPLARNLAAGPYS
                     ISLWQVYPDAGSSMTPHLIVMLDGGAAAPAATDVTVAVNATPTEVRRSMTTSNGWETA
                     EGLAEGDVVAVTISDGIGAWDLDPVVVPPPPTSMLPMQELIYVSIFLTFATAWWMAGR
                     TARAWRRPAVRPT"
     gene            1336..>1540
                     /locus_tag="AC162A_03460"
     CDS             1336..>1540
                     /locus_tag="AC162A_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009925.1"
                     /translation="MTTATRQGFIEKLNGQWHERALWIYLAIVILHWVEHLFQAAQIW
                     ILGMPRPEALGALGYVFPWLVKSE"
BASE COUNT          234 a          511 c          542 g          253 t
ORIGIN      
        1 cctcctggtg atgttccgtg tgatcgccaa cttctccacg acggaggtcg ttctagccct
       61 ccccatcgcc gtgcaggaga tggtcctggc cgtctggctg atcgccaagg gcttcgctcc
      121 ccttgccatc gccgccgagc ccgccggcga gacgtcgcgg aggccggccg gcacgagccc
      181 cgtgaccgtg agctgacccg atgacaacgt cgacgaagcc cgcgtccatg ccgccgcgct
      241 ggatcgttcg cgcggtgtgg gcggtccaca gggcgatcta ctcgatgacc ggtggccggc
      301 gcgggcttcg caacccgacc gcagatcggt ggggcatgat gcgcctgcgg accgtcgggc
      361 gtcgttcggg tcaggagcgg acggccatcc tcgggtactt cgaggatggc caggacctgg
      421 tcacgatggc catgaacggg tggaccgagg gcgagcccgc gtggtggctc aaccttcagg
      481 ctcgaccgga caccgtcgtc gagttgaagg atggcccccg cgtcgtccgc gcccgtgcag
      541 cgaacgcgga agaacggccg cgccggtggg ccaggtgggc gacgtacgac aaggatctcg
      601 aaggctacgc tgcccggcgg tccggcgaga ccgcggtcgt cgtcctgtcg ccccgacccg
      661 attaggcgtg cgtggtccgc tgcgtcggac gcgcggcggg aacggctgac cgtcccggaa
      721 gccaacgtga gacggaaggt tgatcgcgcg ccacggtccc gtttaccgtg gcggacgatg
      781 aaacgacgac tgttcgcagc agcgttcgcc tcggcgatct catgggtcgc gctggccgcg
      841 cccgtctccg cccacggtga ccatgatgcg cggccgctgg cgcgcaacct cgctgcgggg
      901 ccgtacagca tctcgctgtg gcaggtctac cccgatgcgg gttcgtcgat gacgccgcac
      961 ctgatcgtga tgctcgacgg tggagcggca gcccccgcgg cgaccgacgt cactgttgcg
     1021 gtgaacgcaa cgccgacgga ggtccgccgc tcgatgacga cctccaacgg gtgggagacc
     1081 gccgaaggcc ttgcagaggg ggatgtcgtg gcggttacca tctccgacgg catcggagcg
     1141 tgggatctcg atccggtggt cgtccccccg cccccgacct cgatgcttcc catgcaggag
     1201 ttgatctatg tctcgatctt cctgaccttc gccaccgcat ggtggatggc aggaaggacc
     1261 gcccgggcat ggcgaaggcc cgcggtcaga ccgacctgag caagcggcca atgtgttgag
     1321 ggagttggta gcgcgatgac gacggcaacc aggcagggct tcatcgagaa gctcaacggg
     1381 cagtggcacg agcgggccct gtggatctac ctggccatcg tgatcctcca ctgggtcgag
     1441 cacctcttcc aggcggccca gatctggatc ctcggcatgc cgcgccccga ggccctgggt
     1501 gcgctcgggt atgtgttccc gtggctggtc aagtccgagg
//
LOCUS       DBNHXO010000162         3262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2420762, whole genome shotgun sequence.
ACCESSION   DBNHXO010000162 DBNHXO010000000
VERSION     DBNHXO010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2420762"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1581)
                     /locus_tag="AC162A_03465"
     CDS             complement(<1..1581)
                     /locus_tag="AC162A_03465"
                     /inference="COORDINATES: protein motif:HMM:NF015714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR5009926.1"
                     /translation="MSSPRGRLPRGGWLAIGVAGGAAAASSGVPVAAVLVVGLMVFAV
                     SHASLRGSRSARSAGVAAGVGAVALAIRVLLGPTAAATPPLPDDRGPWTATVDSAGSP
                     RDGDQVARLRLHTEGGEVAVAATLPAYPTVAAGDVVEVDGRLRPPPDDDPYGDYLRRS
                     GAAGTLDGRAMTLKEAAPLGLQTLRGASGDALRLALPEPEAGLAAGILVGLRERVDRT
                     LAAEFATAGVSHVVAISGWNIAIVAGLVAGVLRGRSRRVITLGILGTVIAYVVAAGAS
                     PSVVRAAVMAGVALMARESGRAGRAPAALGMAAALLLIVEPAMIEDAGFRLSVMATAG
                     LLAWANPLSEHLGRIAGGRVPRWLADGLGISLAAQAATLPDVLATFGRLSLVSPVVNL
                     AVVPLVPAAMLGGVVAMLAGGLATIGAPPLLATIAGLPGWLVLHLIVLVVRVGASLPF
                     AAVTLPPGIATAAGAAAGIAVLCAPSAWPRVRSLLPARRAVRRPAPTIVAAKSRTTRT
                     IGRSERLGLVGVATVIAIA"
     gene            complement(1578..2279)
                     /locus_tag="AC162A_03470"
     CDS             complement(1578..2279)
                     /locus_tag="AC162A_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIR5009927.1"
                     /translation="MEPTGAPWRALESTEAGSPAPEPAQRGIPWHAMGAVAVAIAVCA
                     VVLLLANRPEPTIEVDGAGAAEDVSGDAGSSFGAGEAVLVVEVGGAVADPGVYRLPAG
                     SRVGDALTAAGGFGPRVDVAQADRQLNLAAPLHDGDEIHVPVRGEAAATGAGAPVGAG
                     DAAAGAAATGPIDLNRATADQLDTLPGIGPVTVAKIVAAREEQPFASIDDLGTRKVVG
                     AATLEKIRALVIVGP"
     gene            complement(2412..3068)
                     /locus_tag="AC162A_03475"
     CDS             complement(2412..3068)
                     /locus_tag="AC162A_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009928.1"
                     /translation="MTQNTRLGLVGAGVLVVAIGASLFGSILTRPNPEPAPVGSPAAQ
                     VDASAPANSVQAPPSSGEPAPSASASLQPTPTPDPEAVRTTAAAAYLAAAGRATDALV
                     AATPRGNPADTRAGAKAEGRRTSEAWGLFVTAMKQLEVPADTKADLRALIRKATAVQE
                     LYVTQPPADGTDAWWSFRREVRNKFGKYEAYVTKVRADLGLPAYCWEPSVCGEDGPPF
                     "
BASE COUNT          458 a         1206 c         1193 g          405 t
ORIGIN      
        1 agcgatcgcg atgacggtgg cgacgccgac caacccgagg cgctcgctgc gtccgatggt
       61 gcgtgtggtc cgtgacttcg cggcgacgat cgtcggggcc ggccggcgca ccgctcgacg
      121 cgccggcagc aggctccgaa cgcgcggcca tgccgacggc gcgcagagga cggcgatgcc
      181 cgccgcggcc ccagcggccg tggcgatgcc cggtggcagg gtcactgccg cgaacggcag
      241 cgaggcgccg acccgcacga ccagcacgat caggtgcagc acgagccagc cggggagacc
      301 ggcgatcgta gcgagcaacg gcggggcgcc gatcgtcgcc aggccaccgg cgagcatcgc
      361 gacgacgccg ccgagcatcg ccgcgggcac cagcggaacg acggcgaggt tgacgaccgg
      421 cgacaccagg gacagccggc cgaacgtcgc gaggacatcc ggcagcgtcg cggcctgcgc
      481 ggccagcgag atcccgaggc catcggcgag ccagcggggc acccgcccgc cggcgatccg
      541 cccgaggtgc tccgagagcg gattggccca ggccagcaac cccgcggtcg ccatgaccga
      601 gagccggaac ccggcgtcct cgatcatcgc cggttcgacg ataagcagga gtgccgcggc
      661 catgccgagc gccgccggcg cgcgtccggc tcgcccgctc tcccgtgcca tgagcgcgac
      721 cccggccatg accgcggccc gcacgaccga cggcgacgct ccggcagcca cgacgtacgc
      781 gatcacggtc ccgaggatcc cgagcgtgat cactcgccgc gatcgcccgc gcaggacgcc
      841 ggccacgagc ccggcgacga tcgcgatgtt ccagccggag atggcgacga cgtggctgac
      901 gccggccgtc gcgaactcgg cggcgagcgt ccgatcgacg cgctcgcgca gcccgacgag
      961 gatcccggcg gcgagcccgg cctcgggctc ggggagggcg agccggagcg catcgccact
     1021 cgcgccgcgg agcgtctgga ggcccaacgg tgcggcttcc ttgagcgtca tcgcccggcc
     1081 gtcgagcgtg ccggccgcgc ccgagcgccg gaggtagtcg ccatacgggt cgtcgtccgg
     1141 cggcggacgc agccgaccgt cgacctcgac gacatcgccc gccgcgaccg tcgggtatgc
     1201 gggcagggtg gccgcgacgg cgacctcgcc gccttcggtg tgcaggcgca gccgcgcgac
     1261 ctggtcccca tctcgtggcg acccggccga gtccacggtc gccgtccagg gacctcgatc
     1321 gtcggggaga ggtggcgtcg ccgcggccgt gggacccagg agcacgcgga tcgccagggc
     1381 gacggctccc accccggccg cgacgccggc actccgcgcc gatcgcgaac cgcgcagcga
     1441 ggcgtgagag acggcgaaga ccatcagccc gacgaccagc accgcggcca ccgggacccc
     1501 gctcgacgcc gcggccgccc cgccggcgac accgatcgcg agccatccac cccgtggcag
     1561 gcggccgcgc ggcgagctca cggtccgacg atgacgaggg ctcggatctt ctcgagcgtg
     1621 gcggcgccga ccactttgcg cgtcccgagg tcatcgatcg acgcgaacgg ctgctcctcg
     1681 cgcgcggcga cgatcttggc aacggtgacc ggaccgatcc cgggcagggt gtccagctgg
     1741 tcggccgttg cccggttgag gtcgatcggg ccagtcgccg ccgcacccgc cgccgcgtcg
     1801 ccggcgccga ccggcgcacc agcgccagtg gccgccgctt cacctcgtac gggaacgtgg
     1861 atttcgtcgc catcgtggag tggcgcggcc aggttcagct ggcggtcggc ctgcgcgacg
     1921 tcgaccctcg gcccgaaccc gccggccgcc gtcaacgcgt ccccgacccg ggatccggcc
     1981 ggcagccggt agacgccggg gtcggcgaca gccccgccca cctcgacgac gagcacggcc
     2041 tcgcccgcgc cgaagctcga accggcgtcg ccggacacgt cctcggccgc acccgcgccg
     2101 tcgacctcga tcgtcggttc aggccggttg gccaggagca ggacgaccgc gcagacggcg
     2161 atcgcgacgg cgaccgcgcc catcgcatgc cacgggatgc cgcgctgggc cggctcaggc
     2221 gccggcgatc cggcctcggt cgattcgaga gcgcgccacg gcgcgccggt cggctccacg
     2281 ggtgagcagc tccccggcgg gccgcaggcg ggcgggtggg gggccatcga tcccgtgggg
     2341 ggcacttccg acgggatcga tgtccatgag aaccctacga gctgcgactt atcccggact
     2401 tatcctcccg gtcaaaatgg cggcccgtcc tcgccgcaca cggacggttc ccagcaatag
     2461 gccggcaggc cgaggtcggc tcggaccttc gtcacatacg cttcgtactt cccgaacttg
     2521 ttcctcactt ctcgtcgaaa gctccaccac gcgtccgtcc cgtcggcggg cggttgggtg
     2581 acatacaact cctgcaccgc ggtggccttc cggatcaggg cccgcaggtc cgccttcgtg
     2641 tcagcaggta cctcgagttg cttcatggcg gtgacgaaca ggccccaggc ctccgatgta
     2701 cgtcggcctt cggctttcgc cccagctcgg gtgtcggctg gattacccct gggcgtcgct
     2761 gcaacgaggg cgtcggtggc gcgaccagcg gccgccaggt acgcggcggc cgccgtcgtc
     2821 cggaccgctt cagggtcggg cgtgggggtc ggctgtagtg aggcggatgc ggatggcgct
     2881 ggctcaccgg agctgggcgg agcctggacg ctgttagcgg gcgccgaggc gtcgacctgg
     2941 gccgctgggc tgcccacggg cgccggctca ggattgggtc gtgtgaggat cgacccgaag
     3001 aggctggcac cgatcgcgac gacgagcacg ccagctccca cgagccctag ccgagtgttc
     3061 tgggtcatgt gcctgttcct ccatggcgcg agccaggcga acctggagcg acgccgtcga
     3121 gttgtcttga gttgggcacg ggccgcccaa agcacgcgat ccgtgagctc ggccgggccg
     3181 gattccaggt aggcctggat ctggcggtcg aggtcgtcgt cggtcgtcat gccagttgct
     3241 ccgcggtggt ggcccggtcg tc
//
LOCUS       DBNHXO010000163         3679 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3214338, whole genome shotgun sequence.
ACCESSION   DBNHXO010000163 DBNHXO010000000
VERSION     DBNHXO010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3214338"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="AC162A_03480"
     CDS             <1..955
                     /locus_tag="AC162A_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009929.1"
                     /translation="RAVLTGGLAGLAAAAAGALVAPAAALAGTDGDVVLGSAANVSAT
                     STKITVPTTGFGAFWGHATGGADSVGVRGDAGGGGVSGAGVWGNGSGSTPGVRGSSPD
                     FVGVFGSGGLYGVVGQSDAGYGIEGDSKSGIGVIGNSDDGPGVLGRSQGDIGVQGQSY
                     SSRGVWGESTKHRGVQGTSHEGWGVYGVSALSDGVYGSSPEAAGVHGASPRGTGVTGE
                     SSQGIGVFCSSNTGLALVTSGRVQVDRVSGVARIPAGKTSVTVSPGVDVVGTAFVLLT
                     PRANVGSRALWFTVNASANTFSIRMSSSRSSATNVAWLLLG"
     gene            987..1565
                     /locus_tag="AC162A_03485"
     CDS             987..1565
                     /locus_tag="AC162A_03485"
                     /inference="COORDINATES: protein motif:HMM:NF023502.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 family protein"
                     /protein_id="HIR5009930.1"
                     /translation="MLLTPRTLAQLSLGLLVVSLAACSGGTTPSTAEGGAGTGAAVSP
                     AGPAGNPPVISGRGGDPCALLSDEEIHRTTGFPVLTRRNASDDQLETRGCRWELDSGT
                     PGTTWTITLDVVDPGGQAYFDENVAIDAGRTNLSEFGERASNQNGIVSVVDGDTLITM
                     FYTQYPSPEGDDDPTIPLLKLILDHVRGTPTS"
     gene            1628..2239
                     /locus_tag="AC162A_03490"
     CDS             1628..2239
                     /locus_tag="AC162A_03490"
                     /inference="COORDINATES: protein motif:HMM:NF023502.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 family protein"
                     /protein_id="HIR5009931.1"
                     /translation="MRLLPLPRSTPSLLPAAFLAISLAACGTVATPSAGSGSSVSGTA
                     SATPGPIDGGATTPPGTPSPTGAGRVLDPCALLTDDEITKTTGFSPISREAVGGDGAL
                     TPGCTWELDSGTPGLTWSISLDVVSPGGQQYYDTFVTIDETLKPVPELGASAGINEFG
                     IVNAVDADTLITLYYIQMPRPKADPTIPLAKLVLEHVRQAPMS"
     gene            complement(2263..2928)
                     /gene="fsa"
                     /locus_tag="AC162A_03495"
     CDS             complement(2263..2928)
                     /gene="fsa"
                     /locus_tag="AC162A_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HIR5009932.1"
                     /translation="MKLFLDTADIDEIRTGVRWGVIDGVTTNPSLYARIKGQTYDEVL
                     QEICALTPGPVSAEVVADDVEGMLVEGRHFAKLADNIVVKVAMSENGLEAIKRFRAEG
                     IKTNCTLIFSANQGLLAAKAGASLLSPFVGRVDDINEEGMIVIRELSEIVNFYDLDAE
                     VLSASIRSPRHITDSALAGAHIATLPFKILQQMVHHPLTDLGITRFKADWEAARAAMA
                     VKA"
     gene            complement(3006..>3679)
                     /locus_tag="AC162A_03500"
     CDS             complement(3006..>3679)
                     /locus_tag="AC162A_03500"
                     /inference="COORDINATES: protein motif:HMM:NF040123.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6518 family protein"
                     /protein_id="HIR5009933.1"
                     /translation="EVAGRPSLAGRAAGGFGASILAGVLLGGSAWFSDQLAWPYSLLV
                     PTNAIGVWVGVAFALGASARTVPTGALRGLVGLLTAVAAYYLLFATLGAGYRAIGAGH
                     AATVWGLVALVAGPVMGGAGATWRHRRGWPRAFAVAILSAALLAEGVMFGLGRLAHLD
                     QIAADPGALLYGIEIVIGAALPGLLLGAGERLRGYGLTVALGAAGALAIGPVTATLRG
                     IADRF"
BASE COUNT          566 a         1315 c         1298 g          500 t
ORIGIN      
        1 gcgagcggtg ctcaccggcg gcctcgccgg gctggctgcc gccgccgctg gtgccctcgt
       61 ggctccggcg gccgccctgg ccggcaccga cggcgacgtc gtcctcgggt ccgcggcgaa
      121 cgtgtcggcg acctcgacca agatcacggt cccgaccacc ggcttcgggg ccttctgggg
      181 ccacgcgacg ggcggcgccg actcggtcgg cgtgcgtggg gatgccgggg gcggcggcgt
      241 cagtggcgcc ggggtctggg ggaatggctc ggggtcgacg cccggcgtcc gcgggagctc
      301 tcctgacttc gtgggcgtct tcgggagtgg aggcctgtac ggcgtcgtcg gacagagcga
      361 cgccgggtac ggcatcgagg gggactccaa gtcgggcatc ggcgtgatcg gcaacagcga
      421 cgacgggccc ggcgttctcg gcaggagcca gggcgacatc ggcgtacagg gccaaagcta
      481 ctcgagccgc ggggtctggg gcgagagcac gaagcatcgc ggcgtccagg ggaccagcca
      541 cgaaggctgg ggcgtctacg gcgtcagcgc actgagcgat ggcgtatacg gctcgagccc
      601 cgaagcagcg ggcgttcacg gtgcgagccc tcgagggacc ggcgtcaccg gcgaatccag
      661 ccagggaatc ggcgtcttct gctcgtcaaa caccggactt gcactcgtga ccagcggacg
      721 ggtgcaggtc gacagggtca gcggcgtcgc gcgcatcccg gccggcaaga cgtcggtcac
      781 cgtcagcccg ggcgtcgacg tcgtgggcac agcgttcgtg ctgctgacac cgcgcgccaa
      841 cgtcgggtcg cgcgcgctct ggttcacggt caatgcctcg gccaacacgt tctcgatccg
      901 gatgagctcg tcccgctcgt ccgccacgaa cgtggcctgg ctcctgctcg gatgaccggg
      961 ctcccgtccc accggaggtc acgctgatgc tcctcacgcc tcgaaccctg gcccagctgt
     1021 ccctcgggct gctggtcgtg tcactcgccg cctgcagcgg tgggacgacg ccgagcacgg
     1081 cggaaggcgg ggcaggtaca ggcgcggcgg tgtcccccgc cgggccggcc ggcaacccgc
     1141 cggtcatcag cgggcgggga ggcgatccgt gtgccctcct gtccgacgag gagatccatc
     1201 ggacgaccgg attcccggtg ctgacgcgcc ggaacgccag cgacgaccag ctggagacgc
     1261 gaggctgccg ctgggagctc gacagcggga cacccggaac gacgtggacg atcaccctgg
     1321 acgtcgtcga tccaggcgga caggcctact tcgacgaaaa cgtcgcgatc gacgcggggc
     1381 gcacgaacct gtccgagttc ggcgagcggg ccagcaacca gaacgggatc gtcagcgtcg
     1441 tcgacggcga cacgctcatc acgatgttct acacgcagta cccgagcccg gaaggcgacg
     1501 acgatccgac gatcccgctc ctgaagctga tcttggatca cgttcgcggg acaccgacct
     1561 catgagtccc gccacgcggg tcgcgttgcg caggcccggt caactcccca tgcccaggag
     1621 gatcacgatg cgactgctgc ctctgccccg gtcaaccccg tcgctgctcc cagcggcgtt
     1681 cctcgccatc tcgctcgccg cctgcggcac ggtcgcgacg ccgagcgcgg gcagtggctc
     1741 gagcgtcagc gggacagcct cggccacgcc cggcccgatc gacggagggg ccacgacccc
     1801 gcccggcacg ccctcaccga ccggtgccgg gcgggtgctc gacccgtgtg ccctgctgac
     1861 ggacgacgag atcacgaaga cgaccgggtt ctcaccgatc tcgcgcgaag ccgtcggcgg
     1921 cgacggcgcg ttgacgcccg gctgcacgtg ggagctcgac agcgggacgc cgggcctgac
     1981 atggtccatc tcgctcgacg tcgtgagccc cggaggccag cagtattacg acaccttcgt
     2041 cacgatcgac gagaccctga agcccgtgcc cgagctcggg gcgtcggccg ggatcaacga
     2101 gttcggcatc gtcaacgccg tcgacgccga cacgctgatc acgctctact acatccagat
     2161 gccgaggccg aaggccgacc cgacgatccc gctcgcgaag ctcgtgctgg agcacgtccg
     2221 ccaagcgcca atgtcctgac cggggcgtcg ctcggccgcc cgctacgcct tgacggccat
     2281 cgcggcgcga gcggcctccc agtcggcctt gaaccgggtg atcccgaggt cggtcagcgg
     2341 atggtggacc atctgctgga ggatcttgaa cgggagcgtc gcgatgtgcg cgccggccag
     2401 cgccgagtcg gtgatgtggc gggggctgcg gatcgaggcc gacagcacct cggcgtcgag
     2461 gtcgtagaag ttcacgatct ccgagagctc ccggatcacg atcatccctt cctcgttgat
     2521 gtcgtccacc cgcccgacga acggcgacag cagggaggcc ccagccttgg ccgcgagcag
     2581 cccctggttc gcggagaaga tcagcgtgca gttggtcttg atgccctcgg cgcggaagcg
     2641 cttgatcgcc tcgaggccgt tctcggacat cgccaccttg accacgatgt tgtcggcgag
     2701 cttcgcgaag tgccggccct cgacgagcat gccctcgacg tcgtccgcga ccacctccgc
     2761 cgagaccggc cccggggtca gggcacagat ctcctggagg acctcgtcgt acgtctgccc
     2821 cttgatcctc gcgtacagcg acgggttggt cgtcacgccg tcgatgacgc cccaccggac
     2881 gccggtgcgg atctcgtcga tgtcggcggt gtcgaggaac agcttcatgg ctggtgctcc
     2941 gttcgcggca cgtgtcttct gggcctgccg attctacgct ggccccgatg aacgccagag
     3001 cgcggtcaga agcgatcggc gatgccccga agggtggccg tgaccggacc gatcgccaac
     3061 gcgccggccg ccccgagcgc gaccgtcagg ccgtacccgc gcaggcgctc gccggccccc
     3121 aggagcagtc ccgggagggc cgccccgatg acgatctcga tcccgtaaag gagcgcaccg
     3181 ggatcggcgg cgatctggtc caggtgcgcg agacgcccga gcccgaacat gaccccctcg
     3241 gcgagcaacg cggcgctgag gatcgcgacc gcgaacgccc gcggccagcc gcgccggtgc
     3301 cgccatgtcg ccccggcgcc ccccatcacg ggtccggcga ccagggcgac cagcccccat
     3361 acggtcgccg cgtgcccggc gccgatcgct cgatacccgg cgccgagggt cgcgaacagc
     3421 aggtagtagg cggccaccgc cgtcagcagc ccgacgagcc cgcgcagcgc cccggttggg
     3481 accgtccgcg ctgaggcgcc gagcgcgaat gcgacgccga cccagacgcc gattgcgttg
     3541 gtcgggacga ggaggctgta gggccaggcg agctgatcgg agaaccaggc actgccgccg
     3601 agaaggaccc ccgccaggat cgacgcgccg aaccccccgg cggcacgtcc ggccagggag
     3661 ggtcggccag cgacctcag
//
LOCUS       DBNHXO010000164         1587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2023922, whole genome shotgun sequence.
ACCESSION   DBNHXO010000164 DBNHXO010000000
VERSION     DBNHXO010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2023922"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="AC162A_03505"
     CDS             complement(<1..502)
                     /locus_tag="AC162A_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024909.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR5009934.1"
                     /translation="MSFESPQLLLVLLFVPLGIYVARAIEARRRERAGAFLGGGPAVR
                     PGAGRRPWRAVLPGALLVAGFVILALAVARPQATVALPRPEGTVMISFDVSGSMAATD
                     LPPTRMDTARTIASTIVERQPAGVVVGLVAFSDAGIAVHAPTSDQADILGAIDRLTPA
                     RGTSVGQ"
     gene            complement(499..1461)
                     /locus_tag="AC162A_03510"
     CDS             complement(499..1461)
                     /locus_tag="AC162A_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR5009935.1"
                     /translation="MAPERVPEAPPEEAPADRRSRPVSAPERVLQRLDWQVVRRLDGL
                     LQGDYRSLFRGSGLDLASLREYEPGDDVRLIDWNVTARTTIPHVRQYHEDREVTAWFL
                     LDLSPSVDFGTVDDGRQKRTVLLDFTATMARLLTRRGNRVGALLFASQVDRTIPASGG
                     RAQVLRIVEDLMDEPRLAHAPVTDLAALFEAAHRTIRRRSMVMIVSDFISAPGWEKPL
                     HLLNRRHEVLAVRLVDPRESELPDVGTIVIEDAETGEQLLVDTGDAKFRRRFAEAAVT
                     RKAELAQAFGRAGVDAVTLSTDEDLVRAIVRMASQRKLRHRSRS"
BASE COUNT          230 a          566 c          589 g          202 t
ORIGIN      
        1 cctgcccgac cgacgtgccg cgggcgggtg tgaggcggtc gatggcgccc aggatgtcgg
       61 cctggtcgct ggtcggcgcg tggaccgcga tccccgcgtc gctgaacgcc acgaggccga
      121 cgacgacccc cgccggctgg cgctcgacga tggtcgacgc gatcgttcgt gccgtgtcca
      181 tgcgcgtggg cggcagatcg gtcgccgcca tgctccccga gacgtcgaac gagatcatca
      241 ccgtcccctc cggccgcgga agggcgaccg tcgcctgtgg acgcgcgacg gccagcgcca
      301 ggatgacgaa cccggcgacc agcagtgccc cgggcagcac ggctcgccac ggtcgtcgtc
      361 cggcgccggg gcggacggct gggccaccac cgaggaaggc ccccgcccgc tcgcgccggc
      421 gcgcctcgat cgcgcgagcc acgtagatcc cgagcggcac gaacagcagc accagcagca
      481 gctgggggga ttcgaagctc acgaccggct ccgatgccgc agcttgcgct ggctcgccat
      541 ccggacgatc gcccgcacca ggtcctcgtc ggtcgacagc gtcactgcat ccaccccggc
      601 ccgcccgaac gcttgggcga gctccgcctt gcgcgtgacg gcggcctcgg cgaagcggcg
      661 ccggaacttg gcgtcaccgg tatcgaccag cagctgctcg cccgtctcgg cgtcctcgat
      721 gacgatcgtg cccacgtccg gcagctcgga ctcgcgagga tccaccagcc ggaccgcgag
      781 cacctcgtgc cggcgattga gcaggtgaag cggcttctcc cagccgggcg cgctgatgaa
      841 gtccgacacg atcatcacca tcgaccgccg tcggatcgtc cggtgggccg cctcgaacag
      901 ggcggcgagg tcggtgacgg gggcatgcgc gaggcgcggc tcgtccatga ggtcctcgac
      961 gatacgcagc acctgggccc gcccgcccga ggctgggatc gtgcggtcga cctggctcgc
     1021 gaacaggagg gcgcccacgc ggttgccccg ccgcgtgagc aggcgcgcca tcgtcgccgt
     1081 gaagtcgagg aggacggttc gcttctgccg tccgtcgtcg accgtcccga agtccaccga
     1141 ggggctgagg tcgagcagga accaggcggt gacctcgcga tcctcgtggt actggcgaac
     1201 gtgcgggatc gtcgtgcggg ccgtgacgtt ccagtcgatc aggcggacgt cgtcgcccgg
     1261 ctcgtactcg cgcagggacg cgaggtccag gccggagccc cggaacaggc tgcggtagtc
     1321 gccctgcaac aggccatcga gccggcggac gacctgccag tcgaggcgct ggaggacgcg
     1381 ttcgggcgcg ctgaccgggc ggctccggcg atcggccggg gcctcctcgg gcggcgcctc
     1441 gggcacccgt tcaggcgcca acgcggacag gagcatggtc ctggaggggg acgaccggcg
     1501 gcgtgacgcg gccgaggatc gtggcgagga tcgcgtccgg cgagacgtcg tccgagaggg
     1561 cctcgaacga gaggacgagc cggtggc
//
LOCUS       DBNHXO010000165         4817 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_635139, whole genome shotgun sequence.
ACCESSION   DBNHXO010000165 DBNHXO010000000
VERSION     DBNHXO010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_635139"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="AC162A_03515"
     CDS             <1..297
                     /locus_tag="AC162A_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009936.1"
                     /translation="GSSGLTVAAFEGSGLDAPAMLDFYAQSARADRHTEKLATSDVTV
                     GGEAGHRLDVLATNGAGHTVVTWPADRANTVFVLLAADLGDAAVLAALDEFAAR"
     gene            333..2111
                     /locus_tag="AC162A_03520"
     CDS             333..2111
                     /locus_tag="AC162A_03520"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR5009937.1"
                     /translation="MSDRPDAWAARRRAWRVERLAPSLERLPERRERFSTLGDVPIEG
                     LYGPWDWVAPDDGEGAGPGGPTAVDHHGDPLREGAGRYAGMDPARDVGLPGEPPFTRG
                     IHPTGYRSRPWTMRMFAGFGSAEDTNARFRQLLEAGQTGLSIAYDMPTLYGYDTDDAE
                     AEGEFGTCGVAVSSLADMELLLDGLPLDRVSTSMTINAPAAPIWAMYIAAAEKRGVPR
                     AALEGTTQNDILKEYVAQKEFLYPPEPSMRLVVDTIEFGSRELPRWNTVSISGYHIRE
                     AGSTAVQELAFTIADGMAYVEACVARGLRVDDFAPRLSFFFNSHSDFFEEIAKFRASR
                     RIWWKLMSERYRAENERSTWMRFHTQTAGVSLTPQQPLNNLTRVATQALAAILGGTQS
                     LHTDAYDEALAVPTAEAALLALRQQQILAEETGVASTVDPLGGSWFVESLTNRVERDV
                     WRYLDEIDRRGGMVAAIAEGYPQREIADAAYRFQREFDEGERVIVGVNGYTNDEPVTV
                     PVLQVPEGSFERHMARLERTRAERDSEAVTAALEGLREAASRPGSSETNLMPHFLRCA
                     ERYATLGEQCSVLREVFGEYREPVSV"
     gene            2134..2619
                     /locus_tag="AC162A_03525"
     CDS             2134..2619
                     /locus_tag="AC162A_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5009938.1"
                     /translation="MYLDALSFLEDERDGFRAYEALDALTDEQLDQPVEAANGWSGRD
                     LMGHIILWQEAGLTAAKELAVGETSASKAWLDAEWEKPGVGDRMNEEGLARFRALPID
                     EVRRLFRETSGELRGYLTVVPESRWIKHSDNQNFFFSETLEHYEDHEGELRAILAAAG
                     R"
     gene            2616..3026
                     /locus_tag="AC162A_03530"
     CDS             2616..3026
                     /locus_tag="AC162A_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009939.1"
                     /translation="MTDDRVDDVDDGLDDDAPLDEVEAAEAAAVAGADADESLDERVG
                     TVLDELEDVTRTRDGEVDRLAVGGRVFAVVGADLLEVALDPRIAKAALATPDTRPSPR
                     GAGWIAFTPAVTDRFALDRAEAWVRLAHRRASGG"
     gene            complement(3086..3463)
                     /gene="trxA"
                     /locus_tag="AC162A_03535"
     CDS             complement(3086..3463)
                     /gene="trxA"
                     /locus_tag="AC162A_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR5009940.1"
                     /translation="MAEIKHATDDTFEALVLHNDKPVVVDFWASWCGPCKMVAPEMEK
                     LAAKYDGLVDVVKVDVDANPRLSQAFGIMSIPTIAYFKPGAQPQGVVGFRPLEQLEQA
                     FSLGSVEAPPATTTVIEAPAPNA"
     gene            complement(3514..3810)
                     /locus_tag="AC162A_03540"
     CDS             complement(3514..3810)
                     /locus_tag="AC162A_03540"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5009941.1"
                     /translation="MIVTQPTTEADRQRSAAVARALGDPKRLCVLESLADGEASVGEL
                     ANRVSCQVPNMSQHLAVLRSAGLVATRRDGNTVYYSLTDPRVIEAYQLLQTIAR"
     gene            3883..>4817
                     /locus_tag="AC162A_03545"
     CDS             3883..>4817
                     /locus_tag="AC162A_03545"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR5009942.1"
                     /translation="MSDAAVTLASIQAMRERLAGRIHHTPMLSSATAARFVEAATGVR
                     LADGRLYLKAEHLQKTGSFKPRAALSRISSLTADERSRGAITISAGNAGQAYAWAGAQ
                     AGVPVTVVMPEGAVASKVAACRGYGAEVVLHGAHVGEALAYLEVLRAERGLTLVHPYD
                     DPEVLIGNGSCGLEILEDLPDVDVVVAGIGGGGLIGGVATAIGELRPSVRIYGVEPVG
                     SEAMTRGLEAGEPVRVLPASVADGLGAPSAGRHALRAVQRYVEDVVLIDDPTILAGLR
                     FGVERLKQVLEPAGSAALAAVLTGAVPLRPGDRVCV"
BASE COUNT          667 a         1660 c         1819 g          671 t
ORIGIN      
        1 gggtcgagcg ggctgaccgt ggcggcgttc gagggcagcg gcctcgacgc gccggcgatg
       61 ctcgacttct acgcgcagag cgcgcgcgcc gaccgccaca ccgagaagct cgccacgagc
      121 gacgtcacgg tcgggggcga ggcgggtcac cggctcgacg tgctggccac gaacggggcc
      181 ggccacaccg tggtgacctg gccggccgac cgggcgaaca cggtgttcgt gctgctcgcg
      241 gccgacctcg gggatgcggc cgtccttgcg gcgctcgacg agttcgccgc gcgctgacgg
      301 atccgccggg atcggatgct agagtcgccg ccatgagcga ccgtcccgac gcctgggcgg
      361 cccgacggcg cgcctggcgg gtcgaacgcc tcgcgccatc gctcgagcgc ctgccggaac
      421 gccgcgagcg gttcagcacc ctgggcgacg tcccgatcga aggtctgtac gggccctggg
      481 actgggtcgc cccggacgac ggcgaggggg cgggacctgg cgggccgacc gcggtcgatc
      541 accacgggga tccgctgcgc gagggcgccg gccggtacgc cggcatggac ccggcccgcg
      601 acgtcgggtt gccgggcgag ccgccgttca cgcgcggcat ccatccgacg ggctatcgct
      661 cccgcccctg gaccatgcgc atgttcgccg gcttcggctc ggccgaggac acgaacgccc
      721 gcttccgcca gttgctcgag gcggggcaga cgggcctatc gatcgcctac gacatgccga
      781 cgctctacgg ctacgacacc gacgacgcgg aggccgaggg cgagttcggg acgtgcgggg
      841 tcgcggtgag cagcctcgcc gacatggagc tgctcctcga tggcctgccg ctcgaccgcg
      901 tgagcacgtc gatgacgatc aacgccccag cggccccgat ctgggcgatg tacatcgcgg
      961 cggccgagaa gcggggcgtg ccgcgggccg cgctcgaggg gacgacgcag aacgacatcc
     1021 tcaaggagta cgtcgcccag aaggagttcc tgtacccgcc ggagccgtcg atgcggctcg
     1081 tggtcgacac catcgagttc gggtcacgcg agctgccgcg ctggaacacg gtctcgatca
     1141 gcggctacca catccgcgag gccggctcga ccgccgtcca ggagctcgcg ttcacgatcg
     1201 ccgacggcat ggcctacgtc gaggcctgcg tcgctcgcgg cctgcgcgtg gacgacttcg
     1261 ccccgaggct gtcgttcttc ttcaacagcc actccgactt cttcgaggag atcgcgaagt
     1321 tccgcgcctc gcggcggatc tggtggaagc tcatgagcga gcgctaccgg gccgagaacg
     1381 agcgctcgac atggatgcgg ttccacaccc agaccgcggg cgtgtcgctg accccgcagc
     1441 agccgttgaa caacctgacc cgcgtcgcga cgcaggccct ggcggcgatc cttggcggca
     1501 cgcagtcgct gcacaccgac gcctacgacg aggcgctcgc cgttccgacc gccgaggccg
     1561 ccctgctcgc gctccgccag cagcagatcc tcgccgagga gacgggcgtg gcctcgacgg
     1621 ttgacccgct cggcgggtcg tggttcgtcg agtcactcac caaccgggtc gagcgcgacg
     1681 tgtggcgcta cctcgacgag atcgaccgcc gcggcggcat ggtcgccgcg atcgccgagg
     1741 gctacccgca gcgcgagatc gccgacgccg cgtaccggtt ccagcgcgag ttcgacgagg
     1801 gcgagcgggt gatcgtcggc gtgaacggct acacgaacga cgaaccggtg acggtgcccg
     1861 ttctccaggt gcccgaaggc tcgttcgagc ggcacatggc gcgcctggaa cggacgcggg
     1921 ccgagcggga cagcgaggcg gtcaccgccg ccctcgaggg gctgcgcgag gcggcgtcgc
     1981 gtcccggctc gagcgagacg aacctgatgc cccacttcct gcgctgtgcc gagcgatacg
     2041 cgacgctcgg cgagcaatgc agcgtcctgc gcgaggtgtt cggggagtac cgggagcccg
     2101 tctcggtctg acgcgcggcg tacgatgccg ggcatgtatc tcgatgccct ctccttcctc
     2161 gaagacgagc gcgacggctt ccgtgcctac gaggcgctcg acgccctgac cgacgagcag
     2221 ctcgaccagc cggtcgaggc ggccaacggc tggtccggcc gcgacctcat gggtcacatc
     2281 atcctgtggc aggaggcggg actgaccgcc gccaaggagc tcgcggtcgg agagacgagc
     2341 gcgagcaagg cgtggctcga cgccgagtgg gagaagcccg gggtggggga ccggatgaac
     2401 gaggaaggcc tcgcccgctt ccgcgcgctg ccgatcgacg aggtgaggcg cctgttccgc
     2461 gagacgtcgg gcgagctccg gggctacctc acggtcgtcc ccgagtcgcg ctggatcaag
     2521 cactcggaca accagaactt cttcttctcc gagacgcttg agcactacga ggaccacgag
     2581 ggcgagctgc gggcgatcct cgcggcggca ggacggtgac cgacgaccgg gtcgatgacg
     2641 tcgacgacgg cctggacgat gacgcgccgc tcgacgaggt cgaggccgcc gaggctgccg
     2701 ccgtggccgg cgcggacgcg gacgaatcgc tcgacgagcg cgtggggacc gtgctcgacg
     2761 agctcgagga cgtgacccgg acgcgcgacg gcgaggtcga ccggctggcg gtcggcggtc
     2821 gcgtgttcgc ggtggtgggc gcggacctgc tcgaggttgc gctggatccg cggatcgcga
     2881 aggcggcgct ggcgacgccg gacacgcgcc cgtcgccgcg cggcgcgggc tggatcgcgt
     2941 tcaccccggc cgtcaccgac cgcttcgcgc ttgatcgcgc cgaggcctgg gtgcgcctcg
     3001 cccaccggcg ggcgagcggc ggctgacgga aacgaagcgg ccccggcagt cgccggggcc
     3061 gtgagggttc tgaggtgcgg cgcgcttagg cgttgggtgc tggcgcctcg atgaccgtcg
     3121 tggtcgcggg gggcgcctcg acggagccca gcgagaaggc ctgctccagc tgctccagcg
     3181 gccggaagcc gacgacgccc tgcggctgtg cgccgggctt gaagtacgcg atggtgggga
     3241 tgctcatgat cccgaacgcc tgcgagaggc gcgggttggc gtccacgtcg accttgacga
     3301 cgtcgaccag gccgtcgtac ttggcggcaa gcttctccat ctcgggggcg accatcttgc
     3361 agggcccgca ccacgacgcc cagaagtcga cgaccacggg cttgtcgttg tgcagcacca
     3421 gggcctcgaa cgtgtcgtcg gttgcgtgct tgatctccgc cacgaggcgg ctcctttctt
     3481 ccccgccgga cggcgggctc tcccagtggc ccgtcagcgg gcgatggtct ggagtagctg
     3541 gtaggcctcg atgacccggg ggtccgtgag gctgtagtag accgtgttgc cgtcgcgacg
     3601 ggtcgcgacg agcccggccg agcgaagcac cgcgaggtgc tggctcatgt tcggcacctg
     3661 gcagctgacc cggttggcca gctcgcccac ggacgcctcg ccatcggcga gggattcgag
     3721 gacgcacagg cgcttcgggt cgccgagggc tcgggcgacg gcggctgagc gctgccgatc
     3781 ggcctcggtg gtgggctggg tgacgatcac gtcctcagtc tctcactggt tgcgcaagaa
     3841 cgcaagtgtt cccggcgccg tgccgacggc atactcagcg ccgtgtccga cgccgcggtg
     3901 accctcgcga gcatccaggc catgcgcgag cgcctcgccg gccggatcca ccacaccccg
     3961 atgctgtcgt cggcgacggc ggcgaggttc gtcgaggccg cgacgggcgt ccgcctcgcc
     4021 gatgggcggc tgtacctcaa ggcggagcac ctccagaaga cgggctcgtt caagccgcgg
     4081 gcggcgctgt cgcggatctc gtcgctgacg gcggacgaac ggtcgcgggg cgcgatcacg
     4141 atctcggccg gcaacgcggg ccaggcgtac gcgtgggcgg gcgcccaggc gggcgtcccg
     4201 gtgacggtcg tcatgccgga gggtgccgtc gcgtcgaagg tcgccgcgtg ccggggctac
     4261 ggcgccgagg tcgtgctcca cggggcgcac gtgggcgagg cactggcgta tctcgaggtt
     4321 ctgcgggcgg agcggggcct gacgctcgtc cacccctacg acgaccccga ggtcctcatc
     4381 ggcaacgggt cgtgcgggct cgagatcctc gaggacctgc ccgacgtcga cgtcgtcgtg
     4441 gccggcatcg gcggcggcgg gctgatcggc ggggtggcga ccgcgatcgg ggagttgcgg
     4501 ccttccgtgc ggatctacgg ggtcgagccg gtcggctcgg aggcgatgac ccgtggcctc
     4561 gaggcgggcg agccggtccg cgtgctcccg gccagcgtcg ccgacgggct gggtgcgccg
     4621 agcgcgggcc gccacgcgct gcgcgccgtc cagcgctacg tcgaggacgt ggtcctgatc
     4681 gacgatccga cgatcctcgc cgggctgcga ttcggcgtcg agcggctgaa gcaggtcctc
     4741 gagcccgccg ggtccgcggc gctcgcggcg gtcctcacgg gcgccgtgcc gctgcgaccg
     4801 ggcgaccggg tttgcgt
//
LOCUS       DBNHXO010000166         7689 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2619712, whole genome shotgun sequence.
ACCESSION   DBNHXO010000166 DBNHXO010000000
VERSION     DBNHXO010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7689)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7689
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2619712"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..483
                     /locus_tag="AC162A_03550"
     CDS             58..483
                     /locus_tag="AC162A_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009943.1"
                     /translation="MNDSTKSDLTVAGVALRFGIVALTLGTAYIHSTLGGLLFTLNAT
                     GYVVAAVAMIVPLALAVRFRWFIRLGLMGYAATAIVMWAIQGPYYSTAYIAKAIEVAL
                     IFLLAIDFLRADGNPVTVVRREIALGLSKVSSIRSASPA"
     gene            531..917
                     /locus_tag="AC162A_03555"
     CDS             531..917
                     /locus_tag="AC162A_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5009944.1"
                     /translation="MKRLLTTVALAAVALAAAACGTTNAGQPAAPAASVDPNAPAVVA
                     KDLKFVTTEVKVPAGTPFGLSFSNQESAPHNVAIYTDSSASQALFKGDIFGNETRVYQ
                     VPALAPGSYFFRCDVHPDMTGTIVAG"
     gene            1154..2077
                     /locus_tag="AC162A_03560"
     CDS             1154..2077
                     /locus_tag="AC162A_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR5009945.1"
                     /translation="MTRRLIAPIAALLALLLLAAPAAAHHGPPRDQFPPRIDLPDGWA
                     PEGITAGKGTTAYVGSLADGAIARVNLRTGDVSPLAAGAPGRVTVGIDYERRAHRLWA
                     AGGATSEVRAYDARTGALLQTYHFTSGFLNDVVVTRHAVYVTDSNVQQLIVIPLRRHG
                     GVPDPSKAFTLPLTGDIAYQDGFNANGIVESRGWLIVVQSNTGQLFRVNPWTGVTAEI
                     EVDGGPVTFGDGMEVIGPWLFVVRNQLNEVAVLRLGHWLKSARVVKTLTSPDLDVPTT
                     LALRAGRLWVVNARFSTPPSPTTEYWLTRLP"
     gene            2173..3093
                     /locus_tag="AC162A_03565"
     CDS             2173..3093
                     /locus_tag="AC162A_03565"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5009946.1"
                     /translation="MLEIRGLTKHYGPVTALDGASLTAHRGRLLGFLGPNGAGKTTTM
                     RCIFGLAVPESGDVHWDGKPVDAATRLRFGYMPEQRGLYPRMRVGEQLAYFAQHHGLS
                     GREANARTATWLERFGLADRAKSKLEDLSHGNQQRIQLATALVHDPELLVLDEPFSGL
                     DPLGVQTMTEILRERTAAGVGVVFSSHQLDLVEEVCEDVVIVSRGKVVADGEINALKA
                     ASGRRHLEVEVAGNGGAWLDGTNDYKVVEREGDWVKLLVDEQADLGSILAAAGAAGEV
                     RRFSFEPPRLSELFMDAVTAPQPSAEDARR"
     gene            3090..4253
                     /locus_tag="AC162A_03570"
     CDS             3090..4253
                     /locus_tag="AC162A_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009947.1"
                     /translation="MSRRLRAIRLVAMREILERGRSRGYVLSLVFTVFLLGAGFVLPS
                     ILLGGDDAPNLALVGSPPAGLQGQIVAVADQYDLSLDISAMPDRAAAEEALRAKTIDA
                     ALDVPSDLSGPGQLIVLEQADDQLRAITAAAVIGLRAGSDAALLSPPTISALEPPTEE
                     QTTAVIFANAGIILMFIGIFSYGTWVLTGVVEEKQSRVVEVVLTTVRPRDLLIGKVLG
                     IGLLALVQLIVLVIAGVIATQLSGRLSLPATTMTAVVQLLVWFVLGFAFYSTAMGFLG
                     SLASRIEEASNATLPVTMLATLSYLLAILVVTQEPDGIVANVLTFLPPSAPMVVPLRA
                     ALDAIEPWQIGLSIALMLVAIWALFVVGGRVYSGAVLQTGGRMKLRDAWRASR"
     gene            complement(4275..4883)
                     /locus_tag="AC162A_03575"
     CDS             complement(4275..4883)
                     /locus_tag="AC162A_03575"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR5009948.1"
                     /translation="MDNDASAGDGRTRCSWGGGPEPDYRAYHDREWGVPIRDERHLFE
                     LLVLEGAQAGLSWWTILRKRDGYRRAFANFDVDAVAAFGEADVERLMADPGIVRNRGK
                     VASAIGNARATLELQAVGSSLIDHLWSFVGGRPVVNAWTTLGGIPAETDESRAMSRDL
                     KARGFKFVGPTICYALMQSAGLVNDHVVTCFRYEEVQAATRA"
     gene            4932..5447
                     /locus_tag="AC162A_03580"
     CDS             4932..5447
                     /locus_tag="AC162A_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIR5009949.1"
                     /translation="MAGESNPYLVLGVRSDASPHDIATAYRALARQHHPDVSQDADAE
                     RRMAEINAAWSTLRDPIRRKAWDRQHAVDVAAAAKAKVESGTWHRGPAGVGAAGPPPG
                     NPRGSVLTFGRHVGWSLGEVARVDPGYLQWLATTRDGARYRDEIDAILATIRQRAEQS
                     NAPTKRRGLFG"
     gene            complement(5444..6004)
                     /locus_tag="AC162A_03585"
     CDS             complement(5444..6004)
                     /locus_tag="AC162A_03585"
                     /inference="COORDINATES: protein motif:HMM:NF042734.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HIR5009950.1"
                     /translation="MIRVYLGHGASGNAASMAPWVDGLRARGIEAHALELPKRKAEDT
                     VAAFEAQVPDEPGAVIGGHSFGGRVASLAVAGVRVPGAAVRVNPYVGLVCLSYPLHRP
                     GGPETADARMAHWPAIEVPALLFSGTSDPFARIDLLEAAMPRLPDGRLVTYPKLGHGL
                     LPVREDVLERMATFLAEVDARSGAPG"
     gene            6108..7469
                     /locus_tag="AC162A_03590"
     CDS             6108..7469
                     /locus_tag="AC162A_03590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR5009951.1"
                     /translation="MVIERIWRTRSVRRSASRLVAVAAVVLGLVVAVPVADAASGTSK
                     VTTLGTSVTFYGKGAGHGVGMSQYGARGRAKAGETATRILAAYYRTARRATTDPDQIV
                     RVLLLDGVPATTDDPLVIHGRDGPWTIDDVEGTFPADAELRVWVPEMTPEDEPPPGPR
                     LEVVDVEGTTLVAAASPAKVVARPADPATRLQLDAKGPGHDTYRGALKIVLEGAVARV
                     INRVGLDDYLRGVLPAEMPSGWPGAALRAQAIASRSFAVRRLHPDTGAFDLFDDTRTQ
                     VYLGVDVETDRTNAIIDGDPGAILVKGKHNRAINAFYHSTGGGATENNEYAFVGASGA
                     VTASPVKYLRGFKDVADDGTPFDAASPYASWKTATLTRARLSTIFGKDARTNVGDLRK
                     LNLSRRGVSGRLYRVTLIGTKGSRTVSKTVSANVFVSVFNAGRPAGSAALRSTLFDTR
                     PIK"
     gene            complement(7483..>7689)
                     /locus_tag="AC162A_03595"
     CDS             complement(7483..>7689)
                     /locus_tag="AC162A_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5009952.1"
                     /translation="TDGLWRGVVYGLEPPLVLLIAAGRDPEGMAANPFFASAPPPIAF
                     VAWSVVWVVLVLAAGIALFRRREL"
BASE COUNT         1083 a         2902 c         2626 g         1078 t
ORIGIN      
        1 cggcgcacca gaagcgaaac gtcaaacccc cgtcagtcca cccggaggcc caccaccgtg
       61 aacgactcga ccaagagcga ccttaccgtc gccggcgtcg cactccgctt cggcatcgtc
      121 gccctgaccc tgggcacggc ctacatccac agcaccctcg gcgggctgct gttcaccctc
      181 aacgccaccg gctacgtcgt cgccgcggtc gccatgatcg tcccgctcgc gctggcagtc
      241 cggttccgct ggttcatccg cctcggcctg atgggctacg ccgcgaccgc gatcgtcatg
      301 tgggcgatcc agggtcccta ctactcgacc gcctacatcg ccaaggccat cgaagtcgcg
      361 ctgatcttcc tgctcgccat cgacttcctc cgcgccgacg gcaaccccgt cactgtcgtc
      421 cgccgcgaga tcgcgctcgg cctgtccaag gtcagctcca tccgcagcgc ctcgcccgcc
      481 tgaacccggc cgcgaccacc cttcgcacgc agtcacgagg aacccgtccg atgaagcgcc
      541 tcctcaccac cgtcgcgctc gcggccgtcg cgctcgccgc ggccgcgtgc ggcaccacca
      601 acgccggtca gccggcggcc ccggccgcct cggtcgaccc gaacgccccg gcggtcgtcg
      661 ccaaggacct gaagttcgtc acgaccgagg tcaaggtccc ggccggcacg cccttcgggc
      721 tctcgttctc caaccaggag agcgcgcccc acaacgtcgc gatctacacg gactcgtcgg
      781 ccagccaggc cctgttcaag ggcgacatct tcggcaacga gacccgggtc taccaggtcc
      841 cggccctcgc gcccggcagc tacttcttcc ggtgcgacgt gcacccggac atgacgggga
      901 cgatcgtcgc cggctgacgg ccgatcgccc cgagcgtcgc ctctcccgcg tccctcgcgc
      961 tccagccccg gtccgcttcg gcggaccggg gcttccgtct gcccgccccg cacctcttgg
     1021 cacgggctgc catgcattcg tcaggacatt gcaaccgttc ggcggcccct ggcgagctga
     1081 tggcatgcga gacggcccga tccttgggac acacctagca ggcgcccgcg cgcgtccccg
     1141 cctggaggat cccatgaccc gacgactcat cgccccgatc gcggcgctgc tcgcgctgct
     1201 cctgctggcc gcgccggccg cggcgcacca cggcccgccc cgcgaccagt tcccgccccg
     1261 gatcgacctg cccgacggct gggcgccgga gggcatcacc gccggcaagg gcacgaccgc
     1321 ctacgtcggc tcgctcgccg atggggcgat cgcgcgggtg aatctgcgca ccggcgacgt
     1381 cagtccgctt gccgccggcg cacccggccg cgtcacggtg ggcatcgact atgaacgccg
     1441 cgcgcatcgc ctgtgggcgg ccggcggcgc gaccagcgag gtccgggcct acgacgcgcg
     1501 gaccggcgcc ctgctccaga cctaccactt cacgtcgggc ttcctcaacg acgtggtcgt
     1561 cacgcggcac gccgtctatg tcacggactc gaacgtccag cagctgatcg tgatcccgct
     1621 ccggcgccac ggcggcgtgc cggatccgtc gaaggccttc accctgcccc tgaccggaga
     1681 catcgcgtac caggacggct tcaacgccaa cggcatcgtg gagtctcgcg gctggctcat
     1741 cgtcgtccag tcgaacaccg ggcagctgtt ccgggtgaac ccctggaccg gcgtgacggc
     1801 cgagatcgag gtcgacggcg ggccggtcac cttcggcgac ggcatggagg tcatcgggcc
     1861 ctggctgttc gtcgtccgca accagctcaa cgaggtcgcc gttctccggc tcggccactg
     1921 gctgaagtcg gcccgcgtgg tcaagacgct gacctcgccc gacctcgacg tcccgacaac
     1981 gctcgcgctc cgggccggcc gcctgtgggt cgtgaacgcc cggttctcga cgcccccgtc
     2041 gccgaccacg gagtactggc tcacgcgcct gccgtagtcg tccctcgcgc tcccgccccg
     2101 gcccgcttcg gcggaccggg gcgtccgttc gcccggcgcg tcggatccgc ccctcgcgat
     2161 aaggtggaga cgatgctcga gatccgcgga ctgaccaagc actacggacc ggtgacggcg
     2221 ctcgacggcg cgtcgctgac ggcgcaccgt ggccggctgc tcgggttcct cgggcccaac
     2281 ggcgcgggca agaccacgac gatgcgctgc atcttcggcc tcgccgtgcc ggagtcgggg
     2341 gacgtccact gggatggcaa gccggtcgac gcggccacgc ggctgcgctt cggctacatg
     2401 cccgagcagc gcggcctgta cccgcggatg cgcgtcggcg agcagctcgc ctacttcgcc
     2461 cagcatcacg gcctgtccgg ccgcgaggcg aacgcgcgga cggcgacctg gctcgagcgc
     2521 ttcggcctgg ccgaccgggc gaagtccaag ctcgaggacc tctcgcacgg caatcagcag
     2581 cggatccagc tcgccacggc gctcgtccat gacccggagc tgctggtcct ggacgagccc
     2641 ttctcgggcc tcgacccgct gggcgtccag acgatgaccg agatcctccg cgagcgcacc
     2701 gcggcgggcg tcggcgtcgt gttcagctcg caccagctcg acctcgtcga ggaggtgtgc
     2761 gaggacgtgg tcatcgtcag ccgcggcaag gtggtcgcgg acggcgagat caacgcgctc
     2821 aaggcggcgt ccgggcggcg gcacctggag gtcgaggtcg cgggcaatgg cggggcctgg
     2881 ctcgacggca cgaacgacta caaggtggtg gaacgcgagg gcgactgggt gaaactgctg
     2941 gtcgacgagc aggcggacct gggttcgatc ctcgccgcgg ccggggccgc cggcgaggtc
     3001 cggcggttct cgttcgagcc cccgcgcctg tccgagctgt tcatggacgc tgtgacggcg
     3061 ccgcagccgt ccgccgagga cgcgcgacga tgagccgccg cctgcgggcc atccgcctgg
     3121 tggcgatgcg cgaaatcctc gaacgcggac gcagccgcgg ctacgtcctg tcgctcgtgt
     3181 tcacggtgtt cctccttggc gcgggcttcg tgctgccgtc gatcctcctg ggcggcgacg
     3241 acgcgccgaa cctcgcgctg gtgggctcgc cgccggcagg cctccagggt cagatcgtgg
     3301 ccgtcgccga ccagtacgac ctcagcctcg acatcagcgc gatgccggac cgcgccgccg
     3361 ccgaggaggc gctgcgagcc aagacgatcg acgccgccct cgacgtcccg tccgacctgt
     3421 ccggcccagg ccagctgatc gtcctcgagc aggccgacga ccagttgcgc gcgatcacgg
     3481 ccgcggccgt catcgggctg cgcgcgggct cggatgccgc cctcctcagc ccgccgacga
     3541 tctcggccct cgagccgccg acggaagagc agaccaccgc ggtgatcttc gccaacgccg
     3601 gcatcatcct gatgttcatc ggcatcttct cctacggcac ctgggtgctg acaggggtcg
     3661 tcgaggagaa gcagagccgg gtcgtcgagg tggtcctcac gaccgtgcgg ccgcgcgacc
     3721 tgctgatcgg caaggtcctg ggcatcgggc tcctcgccct cgtccagctc atcgtgctcg
     3781 tcatcgccgg ggtgattgcc acccagctca gcggccggct gtccttgccc gcgacgacca
     3841 tgacggctgt ggtccagctg ctggtgtggt tcgtcctcgg cttcgcgttc tactcgaccg
     3901 cgatgggctt cctcggctcg ctcgcatcgc ggatcgagga ggcgtccaac gcgaccctgc
     3961 cggtcacgat gttggcgacc ctctcgtacc tgctcgcgat cctcgtcgtc acccaggagc
     4021 ccgacgggat cgtcgcaaac gtcctcacgt tcctgccgcc ctcagccccg atggtcgtgc
     4081 cgctgcgcgc ggcactcgac gccatcgagc cgtggcagat cgggctgtcg atcgcgttga
     4141 tgctggtcgc gatctgggcg ctgttcgtgg tcggcggccg cgtgtactcg ggtgccgtcc
     4201 tccagaccgg cggacggatg aagctgcgcg acgcctggcg cgcctcgcgc tgacctgctg
     4261 cccgacccgc cggctcaggc ccgcgtggcc gcctggacct cctcgtaccg gaagcaggtc
     4321 acgacgtggt cgttgacgag gccggccgac tgcatcagcg cgtagcagat ggtcggcccg
     4381 acgaacttga agccgcgcgc cttgaggtcg cggctcatcg cgcgcgactc gtcggtctcc
     4441 gccgggatcc cgcccagggt cgtccatgcg ttgaccaccg gacggccgcc gacgaacgac
     4501 cacaggtggt cgatgaggct cgagccgacg gcctgcagct cgagcgtcgc ccgcgcgttc
     4561 ccgatcgccg acgcgacctt gccccggttg cgcacgattc ccgggtcggc catcaggcgc
     4621 tcgacgtccg cctcgccgaa cgcggcgacc gcgtcgacgt caaagttcgc gaaggctcgc
     4681 cggtacccgt cgcgcttgcg caggatcgtc caccacgaga ggccggcctg ggcaccctcg
     4741 agcaccagca gctcgaacag gtggcgctcg tcgcgtatcg ggacgcccca ctcccgatcg
     4801 tgataggcgc ggtagtccgg ctccggacct ccgccccagc tgcaccgcgt ccggccgtcg
     4861 cccgccgatg cgtcgttgtc cataccgaag atcctatcgg cagcggctca ccgcgcactt
     4921 atcatcgcgc cgtggccggc gaatcgaatc cctacctggt gctcggcgtc cgctccgacg
     4981 cgagcccgca cgacatcgcg accgcgtacc gggcgctcgc ccgccagcat catcccgacg
     5041 tgtcccagga cgcggatgcc gagcgccgca tggccgagat caacgcggcc tggagcacgc
     5101 tgcgcgatcc gatccggcgg aaggcgtggg accggcagca cgcggtcgac gtggcagccg
     5161 ccgcgaaggc caaggtggag agcgggacgt ggcatcgcgg gccggccggc gtcggggcgg
     5221 ccggaccgcc accgggcaac ccgcgcggca gcgtcctgac cttcgggcgc cacgtcggct
     5281 ggtcgctggg cgaggtcgcg cgcgtcgacc cgggctatct ccagtggctg gcgacgaccc
     5341 gcgacggggc gcgctatcgc gatgagatcg acgccatcct ggccaccatc cgccagcgcg
     5401 cggagcagtc caacgctccc accaagcggc gcgggctgtt cggctagccc ggcgctccgc
     5461 tcctggcgtc gacctcggcg aggaacgtcg ccatccgctc caggacgtcc tcgcgcaccg
     5521 gcagcaggcc gtgcccgagc ttcgggtacg tgaccagccg gccgtcgggc agcctgggca
     5581 tcgcggcctc gaggaggtcg atccgggcga acgggtccga ggtccccgag aacaggagcg
     5641 ccgggacctc gatcgcgggc cagtgggcca tccgcgcgtc cgccgtctcg ggtccgccgg
     5701 gccggtggag cggatacgaa aggcagacca ggccgacgta cgggttgacg cgcacggcgg
     5761 cgccggggac gcgaacgccg gccactgcca gcgaggcgac ccggccgccg aacgagtggc
     5821 cgccgatcac cgctccgggc tcgtccggta cctgcgcctc gaacgccgcg accgtgtcct
     5881 cggccttgcg cttgggcagc tcgagcgcgt gcgcctcgat cccgcgggcg cgcaggccgt
     5941 cgacccacgg cgccatcgac gccgcgttcc ccgacgcgcc atgtcccagg tagacccgaa
     6001 tcacgggacg aggctacccg acgggtgcca tgctggtggc ggtaccccgc ccgggtggtc
     6061 cgtgcgaccc atggccggtg cgtgcgggaa ccaccagact ggggaggatg gtcatcgagc
     6121 gaatctggcg cacgcggtcc gtgcggcgat cggccagccg gctcgtcgcc gtggccgcgg
     6181 tcgtgctcgg cctcgtggtc gcggtcccgg tcgccgacgc cgcctcggga acgagcaagg
     6241 tcacgaccct cggcacgagc gtcacgttct acggcaaggg cgccggtcac ggcgtcggca
     6301 tgagccagta cggcgcgcgg ggccgggcga aggccggcga gacggcgacc cggatcctcg
     6361 ccgcgtacta ccgcacggcc aggcgagcca ccacggaccc ggaccagatc gtgcgcgtcc
     6421 tgctgctcga cggcgtgccc gcgacgaccg acgatcccct ggtgatccac ggccgtgacg
     6481 gcccgtggac gatcgacgat gtcgagggca cgttcccggc cgacgccgag ctgcgcgtct
     6541 gggtgccgga gatgacgccc gaggacgagc cgccgccagg cccgcggctc gaggtcgtgg
     6601 acgtcgaggg cacgacgctc gtcgcggcgg catcgcccgc caaggtcgtc gcgcgtcccg
     6661 cggatcccgc cacccggctc cagctcgacg ccaagggacc ggggcacgac acgtaccgcg
     6721 gcgccctcaa gatcgtcctc gaaggggccg tcgcgcgggt gatcaatcgc gtcggcctcg
     6781 acgattacct gcggggcgtg ctgccggcag agatgccgtc gggctggccc ggggccgcgc
     6841 tccgcgcaca ggcgatcgcc tcccgcagct ttgcggtgcg ccggctccac ccggatactg
     6901 gcgcgttcga cctgttcgat gacacccgga cgcaggtcta cctgggcgtc gacgtggaga
     6961 cggacaggac caacgcgatc atcgacggcg acccgggggc catcctcgtc aagggcaagc
     7021 acaatcgcgc gatcaacgcg ttctaccact cgaccggcgg cggggcgacg gagaacaacg
     7081 agtacgcctt cgtcggggcc tcgggcgcgg tcaccgcctc gcccgtcaag tacctgcgcg
     7141 ggttcaagga cgtcgccgac gacgggacgc cattcgacgc ggcgtcgccc tacgcgagct
     7201 ggaagaccgc gacgctgacc cgcgccaggc tgtccacgat cttcggcaag gacgcccgga
     7261 cgaacgtcgg cgacctgcgg aagctcaacc tcagccggcg cggcgtgtcg ggccgcctgt
     7321 atcgcgtcac gctgatcggc accaagggat cgcggaccgt gtccaagacg gtgtcggcga
     7381 acgtgttcgt cagtgtgttc aacgcgggcc ggcccgcggg cagcgccgcc cttcgcagca
     7441 cgctgttcga cacgcggccg atcaagtagc ggccggggcg gcctacagct cgcgccgccg
     7501 gaacagggcg atcccggccg ccagcaccag caccacccag acaacgctcc aggcgacgaa
     7561 cgcgatgggc ggcggcgcgc tcgcgaagaa cgggttggcg gccattccct cggggtcgcg
     7621 gccggcggcg atgagcagca cgagtggcgg ctcgaggccg tagacgacgc ctcgccacag
     7681 gccgtcggt
//
LOCUS       DBNHXO010000167         4082 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_834759, whole genome shotgun sequence.
ACCESSION   DBNHXO010000167 DBNHXO010000000
VERSION     DBNHXO010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_834759"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="AC162A_03600"
     CDS             <1..195
                     /locus_tag="AC162A_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009953.1"
                     /translation="RVTLHLKQGVNADVPATARVITDAVERRELLVDVAKAWNRTDID
                     RMVEQSPLIELTIEGYAAAA"
     gene            complement(473..928)
                     /locus_tag="AC162A_03605"
     CDS             complement(473..928)
                     /locus_tag="AC162A_03605"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5009954.1"
                     /translation="MGRVGASLEIPYPPEDAFRVATRIEELPRWLPEVVSAVLLDPPL
                     STGSRVRLKLSSAAAGTEVVGTVRQLRPPSALVIGGSGGPLTVEVRARFDPVGTNATR
                     IALEIELTTSPLFGFIGREAERRINAEIPASLQRFRAILDAEAGVTPAS"
     gene            complement(957..1673)
                     /locus_tag="AC162A_03610"
     CDS             complement(957..1673)
                     /locus_tag="AC162A_03610"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HIR5009955.1"
                     /translation="MTIGEPARHLALDGTRNVRDVGGYPAANGRRTRWRTLLRSDELT
                     TLPEHAQEQLTELGLRQVIDLRWPDELVRAPNAFARSASIRYTSLPLLADDPTPHAGL
                     AGMYRHVFDVRSTQLASVARALLEADGLPAIIGCAAGKDRTGVVIAWLLDLVGVPASV
                     IVEDYAVTARYFSAPRSAVDPSDWRHEPLVLECPPEYMAGALEHLDQAHGGARALLRR
                     QGFAEADIDELVERLTEPVR"
     gene            2008..3534
                     /locus_tag="AC162A_03615"
     CDS             2008..3534
                     /locus_tag="AC162A_03615"
                     /inference="COORDINATES: protein motif:HMM:NF015755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR5009956.1"
                     /translation="MTSRGLRSPGVAAFLSLLFPGLGQYAAGERRRGILIAIPALVVL
                     IGIVGFVLGTLLGGGSQAIIGLVLSPEVLVGIVALDLIWLAYHAFAILDAWFVARRLR
                     TDAGRGTSRFAVVGLLAVLAVATLGHGSVAALGMETEDTLSAVFRGDDPNEDPSGDWE
                     IPEASFEPEEDDSPTPAPTASPLPGELIPAPSLVPSVAPTPSPPPVPQWARDGRLNLL
                     LVGSDAGAGRWLLRTDTMVLLSVDIESGRAAMFGIPRNLINVPLAGEDAKAFPDGRFP
                     GLLNALYVYAWGHPSQFPGGDARGFRAVTGAIQELVGVPLDGFVAVDLQGFVKLVNSV
                     KGLWIRIPEPLYDDHYPKVDGSGYITISFKAGCQKLTGGEALSYARSRHQDSDYGRMR
                     RQQLTLLALRRQLDPLALLERAPEMLSIAKGDLWTTIKRKDLASLAQLAADIQARDVD
                     RVYFTPPEYPEYLTTGEIKRIRQTVRHYFDSRGGGGGGGGGGGGNDDGLSNAKPCPDR
                     "
     gene            3674..>4082
                     /locus_tag="AC162A_03620"
     CDS             3674..>4082
                     /locus_tag="AC162A_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009957.1"
                     /translation="MPARSQPRLQAFPAEDLAFRRYVAEAFAQLLDDVSTRPNGRPEA
                     DDLQWRLRVRYPSAVVRERDALADPGGEGQLWYVYRFGSVRPGRRWWEEPGHAWAILD
                     GDRCFVEVSTSLAEIVEVPRATILGQRVEAFANP"
BASE COUNT          585 a         1476 c         1462 g          559 t
ORIGIN      
        1 cgcgtgaccc tccatctcaa gcagggcgtc aacgcggatg ttccggcgac ggcgcgggtc
       61 atcaccgacg cggtcgagcg ccgcgagctg ctggtcgatg tcgcgaaggc gtggaatcgc
      121 accgacatcg accgcatggt cgagcagagc ccgctgatcg agctcacgat cgagggctac
      181 gcggccgccg cctgacgccg gcgcgcgggc tggtctgcca gtccagcgaa tcgggctgag
      241 gcgatcgcgc ggtcgccgca tccttgtgac cccggcctca caggccagat tcctccccga
      301 accggccagc gcacggcccg acgacagccg tcagcgcgaa ttgccggact ggcaggccag
      361 cggtgccgat gccgagccgg cccacggccg ctggccggcc tggcaggcaa gccgcgccag
      421 caacgggcaa gctgaatatc gggaacgaag gagggaactg gggcccaaac gcctatgacg
      481 ccggcgtcac gcccgcctcg gcgtcgagga tcgcccggaa gcgctgcagc gacgccggga
      541 tctccgcgtt gatccgccgc tccgcctcgc ggccgatgaa gccgaacagg ggcgaggtcg
      601 tgagctcgat ctccagggcg atccgcgtcg cgttcgtgcc gaccgggtcg aaccgtgccc
      661 gcacctcgac ggtcagcggc ccgccggacc cgccgatgac gagcgccgac ggcggccgca
      721 gctggcgcac ggtccccacg acctcggtgc ccgcggcggc cgaggagagc ttcaggcgga
      781 cacggctccc cgtcgacagc ggcgggtcga gcaggaccgc gctgaccacc tcgggcagcc
      841 accggggcag ctcctcgatc cgcgtggcga cgcggaaggc gtcctcgggc ggatagggga
      901 tctcgagcga agcaccgacg cgacccatcg acccatccta agccctgcca ctccggtcag
      961 cggaccggtt cggtcaagcg ctcgaccaac tcgtcgatgt cagcttccgc gaatccctgc
     1021 cggcggagca gggcgcgagc gccgccgtgg gcctggtcga ggtgctcgag cgcgcccgcc
     1081 atgtactcgg gcgggcactc gaggaccagg ggctcatggc gccagtcgga cgggtcgacg
     1141 gcggagcgcg gtgccgagaa gtagcgcgcg gtgaccgcgt agtcctcgac gatgaccgac
     1201 gccgggaccc caacgaggtc gagcagccag gcgatgacga cgccggtgcg gtccttgccc
     1261 gcggcgcagc cgatgatcgc gggcagcccg tcggcctcca gcaacgcccg cgcgacgctc
     1321 gccagctggg tcgaacgcac gtcgaacacg tggcgataca tgccggccag gcccgcgtgg
     1381 ggggtcgggt cgtcggcgag cagcgggagg ctcgtgtagc ggatcgacgc ggatcgcgcg
     1441 aaggcattgg gggcgcggac gagctcgtcg ggccagcgga ggtcgatgac ctggcggagg
     1501 ccgagctccg tgagctgctc ctgggcatgc tcgggcagcg tcgtcagctc gtcggagcgg
     1561 aggagcgtcc gccaccgcgt gcgccgcccg ttcgccgcgg ggtagccacc cacgtcgcgg
     1621 acgttccgcg tcccgtcgag cgcgaggtgg cgagccggtt caccgatggt catgcctcga
     1681 gaagcgtacc ggcgatcgcg acgcccgcct gttcatgaca ttcgtccacc ccgggacatg
     1741 accctcggcc cattcggccg agggccggcg cgggctacgg tacggccatc gaacggaccg
     1801 ccaacacacg gacccaatcc ggcggcgcgt cgaccctccg gcgggatgct gggcggcggc
     1861 ggagccggga accttgtccg accagacggt ccgttttcga ttcccggccc acttgcgcgc
     1921 aggcggtatc gtccgcaacg aacgcgaggg gagcgcgtcc atggcgcgta ccgtcgtgtc
     1981 gatgggggca gctgagggcc agttgaagtg accagcaggg gtttgcgctc gccgggcgtg
     2041 gcggcgttcc tgtccctcct gttcccgggc ctcggccagt acgcggccgg cgagcggcgg
     2101 cgcggcatcc tgatcgccat ccccgcgctc gtcgtcctga tcgggatcgt cgggttcgtg
     2161 ctcgggacgc tgctcggcgg cgggtcacag gcgatcatcg gcctcgtcct cagccccgag
     2221 gtcctggtcg gcatcgtcgc cctcgacctc atctggctcg cgtaccacgc gttcgcgatc
     2281 ctcgatgcct ggttcgtggc ccggcgtctg cggaccgacg ccggccgcgg gacgagtcgc
     2341 ttcgcagtcg tcggcctcct cgcggtcctc gccgtcgcga ccctcggcca cgggtccgtc
     2401 gcggcgctgg gcatggagac ggaggacacg ctctcggccg tcttccgggg cgacgacccg
     2461 aacgaggatc cgagcggcga ctgggagatc ccggaggcga gcttcgagcc cgaggaggac
     2521 gattccccga cgcccgcgcc gacggcgagc ccgctgcccg gcgaactcat cccggctccg
     2581 agcctggtgc cgtcggtcgc cccgaccccc agcccgccgc cggtcccgca gtgggcccgc
     2641 gacggccggc tcaacctgct gctcgtcggc tccgacgccg gtgctggccg gtggctgctg
     2701 cgcaccgaca cgatggtcct gctgagcgtg gacatcgagt ctggacgggc ggcgatgttc
     2761 gggatcccgc gcaacctgat caacgtcccg ctcgccggcg aggacgcgaa ggccttcccg
     2821 gacggccggt tccccgggct cctcaacgcc ctgtacgtct atgcgtgggg gcatccctcc
     2881 cagttccccg gcggcgatgc gcgcgggttc cgggccgtca cgggtgcgat ccaggagctt
     2941 gtcggcgtcc ccctcgacgg gttcgtggcg gtggacctcc aggggttcgt gaagctcgtc
     3001 aactcggtga agggcctctg gatccggatc cccgagccgc tctacgacga ccactatccc
     3061 aaggtggacg ggtccggcta catcacgatc tcgttcaagg caggctgcca gaagctgacc
     3121 gggggcgagg cgctgtccta cgcgcgatca cggcaccagg actcggacta cggccggatg
     3181 cgccgccagc aactgaccct cctcgccctg cgtcgccagc tcgacccgct tgccctgctc
     3241 gaacgggcgc ccgagatgct ctcgatcgcg aagggcgacc tgtggaccac gatcaagcgc
     3301 aaggacctcg cgagcctcgc ccagctcgcg gcggacatcc aggcccgcga cgtcgaccgc
     3361 gtctacttca cgccgcccga gtaccccgag tacctgacga cgggcgagat caagcgcatc
     3421 cgccagacgg tccgtcacta cttcgacagt cgtgggggcg gcggaggcgg tggaggcggt
     3481 ggaggcggca acgacgacgg gctgagcaac gccaagccct gcccggaccg ctgacgtgga
     3541 ccggggccgc cgacactcgg agaagcggcc gccgggcatg tgcacccgaa tacgccgtat
     3601 tgctgagcga cgcggctcca cggatcgcgg accatgggcg ccgcttgatc acttcgggga
     3661 accctgatcg accatgcccg cccgctccca gccgcgcctc caggccttcc ccgcggagga
     3721 cctcgccttc cggcggtacg tcgccgaggc gttcgcgcag ctcctcgacg acgtctcgac
     3781 ccgacccaac ggccgtcccg aagcggacga cctccagtgg cggctgcggg ttcgctaccc
     3841 gtccgcggtc gtccgcgagc gcgacgccct cgccgatccc ggcggcgagg gccagctgtg
     3901 gtacgtctac cggttcggga gcgtccggcc cggccgccgc tggtgggagg agccgggcca
     3961 cgcctgggcg atcctcgacg gggatcggtg cttcgtcgag gtgtcgacgt cgctggccga
     4021 gatcgtcgag gtgccgaggg cgacgatcct cgggcagcgg gtcgaggcgt tcgcgaaccc
     4081 cg
//
LOCUS       DBNHXO010000168         2795 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3612718, whole genome shotgun sequence.
ACCESSION   DBNHXO010000168 DBNHXO010000000
VERSION     DBNHXO010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3612718"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..797
                     /locus_tag="AC162A_03625"
     CDS             81..797
                     /locus_tag="AC162A_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009958.1"
                     /translation="MQTQATRDRPPDGQAAVADPDPATRLQDWGFLANPDLPDRPGPA
                     FLLVALRNEPTLRHYDPETIDYWVSSGEHGQRRRLSRSTPMPLSEPFSWGMIRLVDRI
                     HVSNEYLTFGGRLDAAMIDGAIVAVFTSPVPLLRRGGHSQGWDAGADLVGAFFGRLMV
                     AVDFSPGFEATLAAASPVTRYAAFVRDSLRRLPADAATDADRSLTLLIRHEASRLRSD
                     AAADWQGGERLLDATKGDER"
     gene            794..1771
                     /locus_tag="AC162A_03630"
     CDS             794..1771
                     /locus_tag="AC162A_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5009959.1"
                     /translation="MRALLAIDGSGESENALDTAASLVWPPRSRLDIVTVVPADTELL
                     GGPWMFASYDVVPAVRDRLRDEGRRLVDEAAALVRRPGLEVVGRVLEGRAATAITDAA
                     KRTGAQLVIVGARGHGTLERMLLGSVSAEVVDHADCPVLVARQGTAWRILVATDGSPD
                     AELGVGLVAESGVFRYATARVVNVVDVPSAWWLGIGDTAATVEAYSSLAGEARAHGRR
                     VTDDAVARLRAAGIEADGVVSEGSAPTEIVAQAAAWGADLIVVGTRGHGLLMRLIVGS
                     TARTIVQHAPSSVLVARPTAVAPDPRRGHAVRVVAPRASAMPDSTPVPA"
     gene            1781..>2795
                     /locus_tag="AC162A_03635"
     CDS             1781..>2795
                     /locus_tag="AC162A_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HIR5009960.1"
                     /translation="MRIVDASEAVASIRSGEQVYVHCAAATPSVLLDALVARAPELRG
                     VGVVHLHTEGPGPHLAPDMADHFRHRALFIGPNARAAVNEGRADYVPVFLSDVPHLFN
                     SGTIPLDAVFVNATPPDEHGFCSLGTSVEAMHAAVRAATTVIVQFNRSMPRTLGDSFI
                     HVDEIDLAVEVDQPPYEHPTARIGDVERRIGAHVAELVPDGATLQLGIGAIPAATALA
                     LTGKRDLGIHTEMFTDAVVDLVEAGVVNGSRKDRNRGKIVAAFLMGSRRLYDWVDDNP
                     MVEMRPVDFTNDTHVIRSFTRMIAVNSAIEVDLSGQVVADSIGHRLYSGVGGQMDFIR
                     GAAL"
BASE COUNT          367 a         1032 c         1001 g          395 t
ORIGIN      
        1 ctcgaggcgc tgctgcgcgc ccacctggag cgcgaggagc agttcctgct gccgctgctc
       61 gccgaggact gaggcgcgcc atgcagacgc aggcaacccg cgaccggccg cccgacgggc
      121 aggccgccgt cgccgatccg gaccccgcca cccgactgca ggactggggc ttcctggcca
      181 acccggacct tcccgatcgc ccggggcccg ccttcctcct ggtcgcgctg cgcaacgagc
      241 cgacgctccg ccactacgac ccggagacca tcgactattg ggtctcgagc ggcgagcacg
      301 gccagcggcg ccggctctcg cgctccacgc cgatgccact ctcggagccg ttctcgtggg
      361 gcatgatccg gctggtcgac cggatccacg tctcgaacga gtacctcacg ttcggcgggc
      421 ggctggacgc ggcgatgatc gatggcgcga tcgtcgcggt gttcacatca cccgtgccgc
      481 tgctccgccg cggcgggcac tcgcagggtt gggacgccgg tgcggacctc gtgggcgcgt
      541 tcttcgggcg cctgatggtc gcggtcgact tcagcccggg cttcgaggcg acgctggccg
      601 cggcaagccc cgtgacgcgc tacgcggcgt tcgtccgtga ctcgctgcgg cgtctgccgg
      661 ccgatgccgc gaccgacgcc gaccgcagcc tcacgctgct gatccgccac gaggcgagcc
      721 ggctccgatc ggacgcggcc gccgactggc aagggggcga acgcctgctc gacgcgacga
      781 agggagacga acgatgaggg cgctgctcgc gatcgatggc tccggggagt cggagaacgc
      841 cctggacaca gcggccagcc tcgtctggcc accccgatcc cggctcgaca tcgtcaccgt
      901 cgtgcccgcc gacacagagc tgctcggcgg accatggatg ttcgcctcgt acgacgtggt
      961 gccggccgtc cgcgaccggc tgcgcgacga aggccgccgg ctcgtcgacg aggcggccgc
     1021 gctggtccgg cgcccgggtc tcgaggtcgt cgggcgcgtt ctcgaggggc gcgcggcgac
     1081 cgcgatcacc gacgcggcca agcggaccgg cgcccagctg gtcatcgtcg gggctcgcgg
     1141 tcacgggacg ctcgagcgga tgctgctggg atccgtgtcc gccgaggtcg tcgatcacgc
     1201 cgactgcccg gtcctcgtgg cgcgccaggg caccgcatgg cgcatcctcg tcgcgaccga
     1261 cggctcgccc gatgccgagc tcggcgtcgg gctcgtggcc gagtcggggg tgttccgcta
     1321 cgcgaccgcg cgcgtcgtca acgtggtcga cgtcccgtcg gcctggtggc tgggcatcgg
     1381 cgacacggcg gcgaccgtcg aggcgtattc gtcgctcgcg ggcgaagcgc gggcccatgg
     1441 gcggcgcgtg acggatgacg ccgtggcgcg gttgcgtgcc gcggggatcg aggccgacgg
     1501 cgtcgtgagc gagggctccg cgcccaccga gatcgtggcc caggccgctg cgtggggcgc
     1561 cgacctcatc gtggtcggga cgcgagggca cggcctgctc atgcggctga tcgtcggcag
     1621 cacggcgcgg acgatcgtgc agcacgcgcc gtcatccgtc ctcgtcgccc gcccgaccgc
     1681 ggtcgccccc gacccacggc ggggccatgc ggtgcgggtc gtcgcaccac gggcgtccgc
     1741 gatgcccgat tccacgcccg tccccgcgta gtctctcggt atgcgaatcg tcgacgcctc
     1801 ggaggccgtc gcctccatcc gctcaggcga gcaggtctac gtgcactgcg cggcggcgac
     1861 gccctcggtg ctcctcgacg cgctcgttgc ccgagcgccg gagctgcgcg gcgtcggcgt
     1921 cgtccacctg cacacggagg gtccggggcc gcatctcgct ccggacatgg cggaccactt
     1981 ccgccaccgg gcgctgttca tcgggcccaa cgcgcgggcc gcggtcaacg agggccgggc
     2041 cgactacgtc cccgtcttcc tctcggacgt cccgcatctg ttcaactcgg ggacgatccc
     2101 gctcgacgcg gtgttcgtga acgcgacgcc gcccgacgag cacggattct gctcgctggg
     2161 gacgagcgtg gaagcgatgc acgccgccgt ccgcgcggcc acgacggtga tcgtgcagtt
     2221 caaccgctcg atgccccgga cgctcggcga cagtttcatc cacgtcgacg agatcgatct
     2281 cgcggtcgag gtggaccagc cgccctacga gcacccaacg gcccggatcg gcgacgtcga
     2341 gcggcgcatc ggggcgcacg tcgccgagct cgtccccgac ggggccacgc tgcagctggg
     2401 catcggcgcg atcccggccg cgaccgccct ggccctcacc ggcaagcgcg acctcgggat
     2461 ccacaccgag atgttcaccg acgcggtcgt cgacctcgtc gaggcgggcg tggtcaacgg
     2521 gagccgcaag gaccgcaacc ggggcaagat cgtcgcggcg ttcctgatgg gcagcaggcg
     2581 cctgtacgac tgggtggacg acaacccgat ggtcgagatg cggccggtcg acttcaccaa
     2641 cgacacccac gtcatccgct cgttcacccg gatgatcgcg gtcaactcgg cgatcgaggt
     2701 ggacctcagc ggccaggtcg tcgccgactc gatcggccat cgcctgtact cgggcgtcgg
     2761 cggccagatg gacttcatcc gcggggccgc gctcg
//
LOCUS       DBNHXO010000169         1727 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5002299, whole genome shotgun sequence.
ACCESSION   DBNHXO010000169 DBNHXO010000000
VERSION     DBNHXO010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5002299"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="AC162A_03640"
     CDS             complement(<1..862)
                     /locus_tag="AC162A_03640"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF014477.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR5009961.1"
                     /translation="MHTKLNAEAVSSEEAGGEATAAYAGRALGSALRLFVRLAHDDVG
                     KRPAGDVTWDLVRAEFDAVDRALSRFRADSELTALNRLAGTERVVRVSWRLREALALM
                     DRARRVTAGRFDGAVLDSLERIGEHGALLFADDGQSRPPDPVAADLERPRSVRVPVTP
                     LDTGGIGKGLALRWAAVRAMAALPPGSGFLLDAGGDVATAGRAPAGGWRVGIEDPAAR
                     GAADAEPLAVIGVDEGAVATSSISVRHWTDPRGLPVHHIIDPASGEPARTGLIAVTVA
                     DADPAWSEVWT"
     gene            complement(876..1418)
                     /locus_tag="AC162A_03645"
     CDS             complement(876..1418)
                     /locus_tag="AC162A_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009962.1"
                     /translation="MTTERILPAVAGSAPVARAETAAARSGTRSVAGHRSQPFLTMPA
                     RAGILLGASAAIYAVSLAAVAVFQAADDRAVAAGRQPYLDAIAESRAAGDALEARLAT
                     IGASARALAGQWTATSNDIAAYETNLDDLAALVAEVEGSTTALPARIKLPSVSVHGPV
                     ASGGSRTRPSTTTTTRASGR"
     gene            complement(1408..>1727)
                     /locus_tag="AC162A_03650"
     CDS             complement(1408..>1727)
                     /locus_tag="AC162A_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009963.1"
                     /translation="TSQLDDAAAQIAAMRTAIAAANGNVTGDADSASALQRQLDAAAK
                     RLRTVQSQLRGAQARLGQLNAAADRQAALNAAARAAGASTAKSGGGHGGDDEDDEDES
                     DDH"
BASE COUNT          211 a          651 c          635 g          230 t
ORIGIN      
        1 tcgtccacac ctccgaccag gccgggtccg cgtcggcgac ggtcaccgcg atcagcccgg
       61 tccgcgcagg ctcgccggac gccgggtcga tgatgtggtg gacgggcagt ccgcgggggt
      121 ccgtccagtg ccggacactg atcgaggacg tggccacggc accctcgtca acgccgatca
      181 ccgccagtgg ctccgcatcg gcggcgcccc gcgcggccgg gtcctcgatc cccacccgcc
      241 agccaccggc cggcgctcga cccgcggtgg cgacatcccc gccggcgtcg aggaggaatc
      301 cggagcccgg gggcagggcc gccatcgcac gcaccgcggc ccagcggagc gcgagcccct
      361 tgccgatccc gcccgtgtcg aggggcgtca ccggtacccg gacggaccgc ggccgctcga
      421 ggtctgcggc aaccggatcg ggagggcggc tctggccgtc gtcagcgaac aggagggcgc
      481 cgtgttcccc gatgcgctcg agggagtcga ggacggcgcc gtcgaaccgc ccggcggtga
      541 cgcgtctcgc gcgatccatg agcgcgagtg cctcccgcag gcgccaggag acccgaacga
      601 cgcgttccgt cccggccagt cgattgagcg cggtcagctc gctgtccgcg cgaaaccggg
      661 acagggcccg atcgacggcg tcgaactcgg cgcggaccag gtcccacgtc acatcgccgg
      721 ccggacgctt gcctacgtcg tcatgtgcga gccggacgaa cagcctgagc gccgatccca
      781 acgcgcggcc cgcgtaggct gcggtcgcct cgccgccggc ctcctcgctc gagaccgctt
      841 ccgcgttcag cttcgtgtgc acccagtgat cgcgatcagc ggcccgaggc gcgggtcgtc
      901 gtcgtggtgg acggtcgagt ccgcgacccg cccgaggcga cgggcccgtg cacgctcacg
      961 ctcgggagct tgatccgcgc cggcagggcc gtcgtcgatc cctcgacctc ggcaaccagt
     1021 gcggccaggt cgtcgaggtt tgtctcgtac gcggcgatgt cgttcgaggt ggcggtccac
     1081 tggccggcca gggcgcgggc gctcgcgccg atcgtagcga gccgcgcctc gagcgcgtcg
     1141 cccgccgccc gcgactcggc gatcgcgtcg agatacggct gtcggccggc cgcaacggct
     1201 cggtcgtcgg ccgcctggaa cacggcgacg gcggcaagac tcaccgcgta gatggcggcg
     1261 gacgctccga gcaggatccc agcccgcgcg ggcatcgtca ggaacggctg gctccggtgg
     1321 ccggcgacgg accgcgtgcc cgatcgcgcc gccgccgtct cggctcgcgc gaccggcgcc
     1381 gagcccgcga cggcgggaag gatccgctca gtggtcatcg gactcgtcct cgtcgtcctc
     1441 gtcgtcgccg ccgtggccac cgcccgactt ggccgttgag gcaccggccg ccctggctgc
     1501 ggcgttcagc gccgcctggc gatcggccgc ggcgttgagc tgcccgaggc gggcctgggc
     1561 gcctcgcagc tgcgattgaa cggtccgcag gcgcttcgcg gcggcatcga gctggcgctg
     1621 cagcgcgctc gcgctgtccg catcgccggt cacgttcccg ttggccgcgg caattgcggt
     1681 gcgcatggcc gcgatctggg cggcggcatc gtcgagctgg ctcgtca
//
LOCUS       DBNHXO010000170         5918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3812103, whole genome shotgun sequence.
ACCESSION   DBNHXO010000170 DBNHXO010000000
VERSION     DBNHXO010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3812103"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="AC162A_03655"
     CDS             complement(<1..177)
                     /locus_tag="AC162A_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013168.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HIR5009964.1"
                     /translation="MDVIPAIDVEAGRSRVVYWPGASAGVGAPTDRPDRIAEQFVAQG
                     ARILHLVDFDGAQRA"
     gene            complement(183..1862)
                     /locus_tag="AC162A_03660"
     CDS             complement(183..1862)
                     /locus_tag="AC162A_03660"
                     /inference="COORDINATES: protein motif:HMM:NF017745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HIR5009965.1"
                     /translation="MIEQLSGVELPRPAPAPEAGPLDDRLYDLVETRVRRLLIENPTL
                     ATYLGIHTEDDRLGDGSRDAVLGDIAGERAHLAAIEALDDAGLSPAARFERDLELHNV
                     RLRLYELDDIRRWERRATAAGELGDAVFLLFARGAAPLSERLERIADRLEAAPAFLDQ
                     AKTRANGRQVAVWQRTEARYAADLPSLFAEVRAAADGVLEVAALARLDRAIAGARTAL
                     EAYGGWVSETIADASDDWPLGAEQYDELVRLRAFEDLDSDAILEIGWDQLQANLEGRR
                     AAARELDPDADLASVIDRLKSDQPASFEAALDGYREVMARARAYLIEHDLATIPDDER
                     IEVIATPEYLRSVMPFAAYFAPARFDADQRGIYVVTPAVDNDPNAMREHYWGAISNTS
                     IHEAYPGHHLQLAVASHHPSLTRMLAEAPEFTEGWGMYSEQMMREQGFDAGPAFRVSM
                     YTDAVWRACRIILDVRMHRGELTPEEAIDFLVEHTGFEVANARAEVRRYTYTPGYQLS
                     YLLGKVLILGLREDERRRRGADFSLKGFHDTLLRNGSIPISFHRRLLRAEA"
     gene            complement(1904..3025)
                     /locus_tag="AC162A_03665"
     CDS             complement(1904..3025)
                     /locus_tag="AC162A_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HIR5009966.1"
                     /translation="MTLIDLRSDTVTHPTDEMRRAMAAAELGDDMFGDDPTVNALEAR
                     AAELLGKDAGLFVTSGTQGNLVSLMAHLPRGYEAIAGASSHTVLDEAGGHAVVVGATI
                     RALPENPDGTIDPAAIIDAFRDPTDLHEPITGLVALENTHAHSGGRPLSADYTRTVAA
                     LAHERGVPLHVDGARFFNAVVALGVTSAELAAPADSVTFCLSKGLSAPVGSLVVGNGP
                     FIARARRARKLLGGGLRQVGVLAAAGLVALRDGDAGMIERLAEDHANARRLAEGLASL
                     DGIRSPGDIAQPGDGPLDPGRTVTDFVVFRVDRDRAAFLEALEARGVLMVTYPHGQVR
                     AVTHYGVTADDVERTIGAVAAALRDTSPRPAGDPATSAA"
     gene            complement(3078..4316)
                     /locus_tag="AC162A_03670"
     CDS             complement(3078..4316)
                     /locus_tag="AC162A_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR5009967.1"
                     /translation="MSDDPAQATQGDAVGPGPGYVQLVDVTAGPIEAAEAIEQPDEQG
                     RVPVLVRIRRQPPINPLWILVAIGLAASGLFLPLVAALRAVIIVAAVVAILVGIASRL
                     FMRVPPGTVGLVAKGGRHSAVLENGIHRVNPVVALTHVVTTRELAFDVPVAAVRSVDG
                     VNVNVDLVLTLGIGDPVKLAYSITTSDLDEFIHATSQEAVRLLIRRMEALNALDLGAD
                     EAGFLRTAIDAKLAAYGVNVRNVAFTRVLLSEAITASLEARRLATIQLSEEKENFALE
                     ERRLNDRAALIALDQDSRRKALELESLAEALRFQKMEERLTAYPTAARYDVEMQRLKV
                     AQQLAGNSRAVVSLGGNDLVSGLLTAQQAGSLGATVPVAAPPSAVEASTPDDGAEAAD
                     GESGRARGPARGTIRRRTSG"
     gene            complement(4313..5629)
                     /locus_tag="AC162A_03675"
     CDS             complement(4313..5629)
                     /locus_tag="AC162A_03675"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR5009968.1"
                     /translation="MTLSGRPEARARARVRWADLRNRLESVSPEAIGRGLLAAAVIAL
                     AVSLAVGSWPALAPFVVGAVIAYAVLPLANRLDRYMPRVLAAFIAEIVAVAILAVAVL
                     AIVPPLVNGVIQVAGRLPTRDEVQASLASFEASLGQLQEPIRGIVLAVVTEATSTLRS
                     TMDGAVAGAGQFVANQILGVAGTLSFLLGLLVIPAWVLTMVADERSIKERGSRLIAPA
                     IRGDVVALARIVDRAFGTFLRTRVLLAVVVGVLVWLGLELLARVGIAEFRYQVTASVL
                     LGGLQLIPELGYVLGFFPIVLVLAIAGPIPAAAVAVVYVVANRIGSDLIETRVSRGVL
                     DVHPGLLIPAIVVLSQFGGLWLLAAAPLVAILRDLTRYMAGRLGDPAAPANVLPGERA
                     PKTRSASAARVPSVYRAAVPPPISAARSVASPSQPLAPIPERSAAT"
BASE COUNT          847 a         2110 c         2180 g          781 t
ORIGIN      
        1 cgcgcgctgg gcgccgtcga agtccacgag gtgcaggatc ctcgcgccct gggcgacgaa
       61 ctgctcggcg atccggtccg gccggtccgt cggcgccccg acgccggcgg acgcgcccgg
      121 ccagtacacg acccgcgacc gccccgcctc gacgtcgatc gcgggtataa cgtccaccgg
      181 cgtcaggcct cggcccgcag caggcggcga tggaagctga tcgggatcga tccgttgcgc
      241 agcagcgtgt cgtggaaccc cttgagcgag aagtccgccc cgcgccgtcg ccgctcgtcc
      301 tcgcgcaggc ccaggatcag caccttgccc agcaggtagc tcagctggta gccgggcgtg
      361 taggtgtagc gccgtacctc cgcccgggcg ttcgcgacct cgaagccggt gtgctcgacg
      421 aggaagtcga tcgcctcctc gggcgtcaac tcgccccggt gcatccgcac gtcgaggatg
      481 atccggcagg cccgccatac ggcgtcggtg tacatgctga cgcgaaacgc cgggccggcg
      541 tcgaagccct gctcgcgcat catctgctcg gagtacatgc cccagccctc ggtgaactcg
      601 ggtgcctcgg cgagcatccg ggtcagcgac ggatgatgcg atgcgaccgc cagctgcagg
      661 tggtggcccg ggtacgcctc gtggatgctg gtgttcgaga tcgcccccca gtagtgctcg
      721 cgcatcgcgt tggggtcgtt gtcgacggcc ggggtgacga cgtagatgcc gcgctggtcg
      781 gcgtcgaagc gggccggcgc gaagtaggcg gcgaacggca tcaccgagcg caggtactca
      841 ggcgtggcga tgacctcgat ccgctcgtcg tcggggatcg tcgccaggtc gtgctcgatc
      901 agataggcgc gtgcccgggc catgacctcg cggtagccgt ccagcgctgc ctcgaatgac
      961 gcgggttggt cgctcttgag ccggtcgatg acgctcgcga ggtcggcatc gggatcgagc
     1021 tctcgtgcgg cggcccggcg gccctcgagg ttcgcctgca gctggtccca cccgatctcg
     1081 aggatcgcgt cggaatcgag gtcctcgaac gcccgcagcc ggacgagctc gtcgtactgc
     1141 tcggcgccca gcggccagtc gtcggatgcg tcggcgatcg tctccgacac ccagccgccg
     1201 tacgcctcga gcgccgtgcg cgcccccgcg atcgcccggt cgagccgcgc cagcgcggcc
     1261 acctcgagca cgccgtcggc cgcggcccgg acctcggcga acagcgatgg caggtccgcc
     1321 gcgtagcgcg cctccgtccg ctgccagacc gcgacctggc gcccgttcgc acgcgtcttc
     1381 gcctggtcga ggaacgccgg cgcggcctcg agccggtccg cgatccgctc caggcgctcc
     1441 gacagcggcg ccgccccgcg tgcaaacagc aggaacacgg cgtcgcccag ctctccggcc
     1501 gccgtcgcgc gacgctccca gcgccggatg tcgtccagct cgtacaggcg caggcgcacg
     1561 ttgtggagct cgagatcccg ctcgaacctg gccgccgggg agagacccgc atcgtcgagc
     1621 gcctcgatcg cggcgaggtg ggcccgctcg ccagcgatgt cgcccagcac ggcgtcccgg
     1681 ctcccgtcac cgaggcggtc gtcctcggta tggatgccga ggtaggtcgc gagggtcggg
     1741 ttctcgatga gcaggcgccg gactcgtgtc tcgacgaggt catacaggcg atcgtcgagc
     1801 ggtcccgcct cgggtgccgg cgcgggccgc ggaagctcga ccccggaaag ctgctcgatc
     1861 atggttcctc cggcgcccgc gttggctgcg gcgccactcg tggtcaggcg gcgctcgtcg
     1921 ccgggtcgcc cgcgggccgc ggggaggtgt ctcggagcgc cgcggcgacc gcgccgatcg
     1981 tccgctcgac gtcgtcggcc gtcacgccgt agtgggtcac ggcgcgcacc tggccgtgag
     2041 ggtaggtcac catcagcacg ccgcgagcct cgagcgcctc gaggaacgcc gcccggtcgc
     2101 gatccaccct gaacacgacg aagtcggtca ccgtgcggcc cggatcgagc ggcccgtcac
     2161 cgggctgcgc gatgtcgccc ggggagcgga tcccgtcgag gctcgccagc ccctcggcga
     2221 ggcggcgggc gttggcgtga tcctcggcca ggcgctcgat catgcccgcg tcgccgtcgc
     2281 gcagcgccac gagtcccgcg gccgccagca cgccgacctg gcgcaggccg ccgcccagca
     2341 gtttgcgcgc gcgtcgcgcc cgcgcgatga acggcccgtt gccgaccacc agcgagccga
     2401 ccggtgccga gaggcccttc gagaggcaga acgtgacgct gtcggccggg gcggcgagtt
     2461 cggccgacgt gacaccgagg gcgacgaccg cgttgaagaa ccgggcgccg tcgacgtgca
     2521 ggggaacgcc gcgctcgtgc gcgagggccg cgaccgtgcg cgtgtagtcg gcgctcagcg
     2581 gccggccgcc cgagtgggcg tgcgtgttct cgagggcgac caggccggtg atcggctcat
     2641 ggaggtcggt cgggtcgcgg aacgcgtcga tgatcgccgc cggatcaatc gtcccgtcgg
     2701 ggttctccgg cagcgcccgg atcgtcgccc cgaccacgac ggcgtgtccg ccggcctcgt
     2761 cgagcacggt gtgggaggac gccccggcga tcgcctcgta gccgcgcggc aggtgggcca
     2821 tcagcgagac gaggttgccc tgcgtcccgc tcgtgacgaa caggccggcg tccttgccca
     2881 ggagctccgc cgcgcgcgcc tcgagggcgt tgaccgtggg gtcgtcgccg aacatgtcgt
     2941 cgccgagctc ggccgcggcc atcgcccgtc gcatctcgtc ggtcgggtgg gtgaccgtgt
     3001 cggagcggag atcgatcaac gtcatcgccg ggcgagcata gcgcgggtgc gcgcgcggac
     3061 gccggagggc cggcccgtca gcccgaggtc cgccgccgga tcgtgccgcg cgcggggccg
     3121 cgggcgcgcc cggactcgcc gtcggcggcc tccgccccat cgtccggtgt tgacgcttcg
     3181 accgcactcg gcggcgcggc aaccggaacc gtcgcgccga ggctgcccgc ctgctgcgcg
     3241 gtgaggagcc cggacacgag gtcgttgccg ccgagcgaga cgaccgcccg cgagttgccc
     3301 gccagctgct gggcgacctt gaggcgctgc atctcgacgt cgtagcgcgc ggccgtgggg
     3361 tacgcggtca gccgctcctc catcttctgg aaccggagcg cttccgcgag cgactcgagc
     3421 tcgagggcct tccggcgcga gtcctggtcg agcgcgatca gcgcggcgcg atcgttgagc
     3481 cgccgctcct cgagggcgaa gttctccttc tcctcggaca gctggatcgt ggccaggcgc
     3541 cgcgcttcga gggacgcggt gatcgcctcc gacagcagca cgcgcgtgaa ggcgacgttg
     3601 cgcacgttga cgccgtaggc ggcgagcttc gcgtcgatcg cggtccgcag gaagccggcc
     3661 tcgtcggcgc cgaggtcgag ggcattgagc gcctccatcc tgcggatgag tagccggacc
     3721 gcctcctggg aggtggcgtg gatgaactcg tcgagatcgc tggtggtgat cgagtacgcg
     3781 agcttgaccg ggtcaccgat cccgagcgtc agcacgaggt cgacgttgac gttgacccca
     3841 tcgaccgagc ggaccgccgc gacgggcacg tcgaacgcga gctcgcgcgt cgtcacgaca
     3901 tgcgtcaggg cgacgacggg gttgacccgg tggatcccgt tctcgagcac ggccgagtgg
     3961 cggccgccct tcgcgaccag gcccacggtc ccgggcggga cccgcatgaa caggcgcgac
     4021 gcgatcccga ccaggatggc gacgaccgcg gccacgatga tcacggcgcg cagcgccgcc
     4081 accagcggca ggaacaggcc cgacgcggca agaccgatcg cgaccaggat ccagagcgga
     4141 ttgatcggcg gctggcggcg gatgcggacg aggacgggaa cccgaccctg ctcgtcgggc
     4201 tgctcgatgg cctcggcggc ctcgatcggc cccgccgtca cgtcgacgag ttggacgtac
     4261 ccgggtccgg ggccgacagc gtccccctgg gtggcctgcg cggggtcgtc gctcacgtgg
     4321 cggcactcct ctcagggatg ggggcgaggg gctggctcgg cgaggcgacg gaccgtgccg
     4381 cgctgatcgg cggcggcacg gccgcgcggt agacggacgg gacgcgcgcg gcgctcgccg
     4441 aacgcgtctt cggggcgcgc tccccgggca ggacgttggc gggcgccgcc ggatcgccga
     4501 gccgcccggc catgtagcgc gtcaggtccc gcaggatcgc gaccaacggc gcggcggcca
     4561 acagccacag cccgccgaac tggctgagca ccacgatggc cgggatgagc agccctggat
     4621 ggacgtcgag gacgccgcgc gacacgcgcg tctcaataag gtcgctgccg atccggttgg
     4681 cgaccacata gaccaccgcg acggccgccg caggaatcgg tcccgcgatg gcgaggacca
     4741 gcacgatcgg gaagaacccc aagacgtagc cgagctcagg gatcagctgc aggccaccga
     4801 gcagcaccga cgccgtcacc tggtagcgga actcggcgat gccgacccgg gccaggagct
     4861 cgaggccgag ccacacgagg accccgacca cgacggcaag gaggacgcgg gtccgcagga
     4921 acgttccgaa cgcccggtcg acgatccggg ccagcgcgac aacgtcgccc cggatcgcgg
     4981 gggcgatcag gcggctcccg cgctccttga tgctgcgttc gtcggccacc atggtcagca
     5041 cccacgcggg gatcacaagc aggcccagca ggaagctcag cgtcccggcg acgccgagga
     5101 tctggttggc gacgaactgc ccggcacccg ccacggcgcc gtccatcgtc gaccgcaggg
     5161 tcgacgtggc ttcggtgacg accgcgagca cgatgccgcg gatgggctcc tggagctgac
     5221 cgagcgaggc ctcgaagctc gcgagcgacg cctggacctc gtcgcgcgtc ggcagtcgtc
     5281 cggcgacctg gatcacgccg ttcacgagcg gcggaacgat ggcgagcacg gcgacggcga
     5341 ggatcgcgac ggcgacgatc tcggcgatga atgccgcgag gacgcgcggc atgtaccggt
     5401 cgagccggtt cgcgagcggc aggaccgcgt acgcgatgac cgcaccgacg acgaacggcg
     5461 cgagggcagg ccacgagccc accgccagcg acacggcaag cgcgatgacg gcggccgcga
     5521 ggagtcctcg cccgatggcc tcggggctga ccgactcgag ccggttgcgg aggtctgccc
     5581 agcgaaccct ggcgcgtgcg cgtgcctcgg gacgcccgga gagggtcata tggcgcgatg
     5641 cccgctggtc atgtggatct cccaggtcgc gatccggatt ccggagcggc acgacctcga
     5701 accgagtata tgaccgcggc tcgtggacgc atcacgcagc gaagacgacg cgccgggagg
     5761 gcatcggcgt ggcccagatg gcgtgctcgg aaacccctca ggatcgctgg cggcgccatg
     5821 gggtccctgc tatcatccgc caccggctgc ggacccgtgg tgtagcggcc caacatgcca
     5881 gcctgtcacg ctggagatcg ccggttcgaa tccggtcg
//
LOCUS       DBNHXO010000171         1604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_835701, whole genome shotgun sequence.
ACCESSION   DBNHXO010000171 DBNHXO010000000
VERSION     DBNHXO010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_835701"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="AC162A_03680"
     CDS             <1..498
                     /locus_tag="AC162A_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family phosphohydrolase"
                     /protein_id="HIR5009969.1"
                     /translation="VSAGIDIAYKSGLPKALIAFIPQHHGTAVMSYFYARAKEQAAEP
                     YGGPATAEGKKAADAVDIRKFRHGGPKPQTREAAIIMLADSVEASVRSLASRDEAAIR
                     VMVSRIFEERIADDQFDECDLTLRDIERIREAFVEQLLGMYHQRIAYPQNKVVEIESR
                     RASGE"
     gene            533..1075
                     /gene="ybeY"
                     /locus_tag="AC162A_03685"
     CDS             533..1075
                     /gene="ybeY"
                     /locus_tag="AC162A_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR5009970.1"
                     /translation="MRSLYLRPWRIDVDWRDGVPRLLPSTVLARTMAAALDAAGAPRP
                     ASIGLILADDRELASLNAEHMGKAGPTDVLSFPLLPPSAYPAHSGQRPGVRSTNGGVS
                     FALPPDVRPHLGDVIVSVERAIEQADAGRGGQTGDVRWDARDELRLLVTHGTLHVCGW
                     DHAEPAEEAAMRALERRLLS"
     gene            1072..>1604
                     /locus_tag="AC162A_03690"
     CDS             1072..>1604
                     /locus_tag="AC162A_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009971.1"
                     /translation="MTPPAPRVRAAYVLVLVATVLAGGCSLLPGAPGEAPAVGGAPVQ
                     YTCGRFPFGAEMLDRDGGDEAAANPAAAALRDQLAKDSPEMNALPDHGWSLAGIDADG
                     AEFVVTGGDLGMRVVTVNSHDPGPWLVTGWGDCQPQRVPPPGLGEASWTLDPEEPKPG
                     ADSQTFTALVTERDCASG"
BASE COUNT          228 a          568 c          584 g          224 t
ORIGIN      
        1 gtgagcgcgg gcatcgacat cgcctacaag tcggggctgc ccaaggcgct gatcgcgttc
       61 atcccgcagc accacgggac ggccgtgatg tcctacttct acgcccgggc gaaggagcag
      121 gcggccgaac cctacggggg cccggcgacg gccgagggca agaaggcggc cgacgcggtc
      181 gacatccgca agttccgcca cggcggcccc aagccccaga cgcgagaggc cgcgatcatc
      241 atgctggccg actcggtcga ggcgtccgtg cggtccctgg cgtcacgcga cgaggcggcg
      301 atccgcgtga tggtctcgag gatcttcgag gagcggatcg ccgacgacca gttcgacgag
      361 tgcgacctga cactgcgcga catcgagcgg atccgtgagg cgttcgtcga gcagctgctc
      421 gggatgtacc accagcggat cgcctacccg cagaacaagg tggtcgagat cgagtcgagg
      481 cgggcctccg gcgagtaggc cgcgtcaggg tcggcgcgct accgtgtcgg cgatgcgaag
      541 cctgtacctg cgtccgtggc ggatcgacgt ggactggcgc gacggcgtcc cgcgcctgct
      601 tccctcgacc gtgctggccc gcacgatggc cgccgcactg gacgccgccg gtgcgccgcg
      661 gcccgcctcg atcggtctca tcctcgccga cgaccgcgag ctggcctcgc tcaacgccga
      721 gcacatgggc aaggccgggc cgaccgacgt gctctcgttc ccgctgctcc cgccgtcggc
      781 gtatccggcg cattccggtc agcggcctgg cgtccgatcg acgaacgggg gcgtgtcatt
      841 cgccctgccg ccggacgtgc gtccgcacct cggtgatgtg atcgtctcgg tggagcgggc
      901 gatcgagcag gccgacgccg gccgcggcgg ccagaccggc gacgtccgct gggatgctcg
      961 ggacgagctc cgcctgctcg tcacccacgg cacgctccac gtgtgcggct gggaccacgc
     1021 ggagccagcc gaagaagcgg caatgcgcgc gctggagcgc cggctgctct cgtgacaccg
     1081 ccggcgccgc gagtccgagc cgcgtacgtc ctggtcctcg tcgccaccgt cctcgcgggc
     1141 ggctgcagcc tcctgccggg ggcgcctggc gaggccccgg cggtcggcgg ggctcccgtc
     1201 caatacacgt gtggtcgatt cccgttcggt gccgagatgc tcgaccgcga cggcggcgac
     1261 gaggcggcgg cgaaccctgc cgcggccgcg ctgcgcgatc aattggccaa ggacagccct
     1321 gagatgaacg ccctgccgga tcacggctgg tcgctggccg ggatcgacgc cgacggcgcc
     1381 gagttcgtgg tcaccggtgg cgatctgggg atgcgggtgg tcacggtcaa ctcccacgat
     1441 cccggtccct ggctggtgac tgggtggggc gactgtcagc cgcaacgagt acctccgccg
     1501 ggactcggcg aggccagctg gacgcttgac ccggaggaac cgaagccggg cgccgactcg
     1561 cagacgttca cggctctcgt gaccgagcgc gactgcgcca gcgg
//
LOCUS       DBNHXO010000172         2987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_637739, whole genome shotgun sequence.
ACCESSION   DBNHXO010000172 DBNHXO010000000
VERSION     DBNHXO010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_637739"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1504)
                     /locus_tag="AC162A_03695"
     CDS             complement(<1..1504)
                     /locus_tag="AC162A_03695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR5009972.1"
                     /translation="MTGEPSDPPARTPLALAGRSLLGVLHNRDIRSLELAWTLGVAAD
                     WALLVVALLVAYDEGGAVLVGLVSLTRMIPATVVNVVIDTSAFPRPERALIGANLVRA
                     AGAVVVAAASLADATALVFVAVAAASAAGALVRPTVLTLLPAVATSPAELVSANTAGA
                     LGESLGTFAGPLVAGLVIAQGGAAPAAVVSGVLCLVAAGVAAAVRVPDASRLRDRERR
                     ASIPLVAGVRELVARPPAGMVMVSFIAQTAVRGALTTFLAILAIEVLGMGDAGVGVLG
                     AAIGLGGIAGALGALAIGAERRLAVVFAIALVLWGAPIAVIGFVPVTVVALLALGVVG
                     VGNALIDVSGLTLLQRGTSNRARSAVFAVLEVVASASVSIGAIVASLLIGTLGIERAL
                     VLVGLSLPVVAVLGWPLVRRLDTEGVVPERQASLLRGIPLFAMLPLAALERVATGMRE
                     VRFAAGERLMSQGEEGDTFVVIERGDVEVAMDGQPHHREGAGAGIGEIALL"
     gene            complement(1504..>2987)
                     /locus_tag="AC162A_03700"
     CDS             complement(1504..>2987)
                     /locus_tag="AC162A_03700"
                     /inference="COORDINATES: protein motif:HMM:NF017745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HIR5009973.1"
                     /translation="SMLRESIASDIAELDTGLLDWNVDPIEGVPAMFLLVPDYQRLDT
                     PEDGLRMVARWRAMAAHTDRHLGNLRRSLAGGRIASRPPTERTIAILDDLLRDDTAAW
                     PLLAPLRTVTDQPGWSTSDRERFAESLQRAVEDEIRPAFVRLRDALVDEILPRARPAE
                     RPGMGEVPGRPDAYRELIRVHTSLDLDAAEIHRVGLDEIARIDAELTDLAGRTIGTSS
                     LGDALSHLRSDPALHFATRDEVFAKAAWCLARATEAIPDWFGRLPEAPCEVVRMGPRE
                     EEHSTIAYYRQPATDGSRPGSYYINTSAPTTRPRYEAESLAYHEAVPGHHLQIAIGQE
                     LPHIPEFRRHLGPTAFFEGWGLYTERLSDEMGLYSGDLDRIGVLSFDAWRAARLVVDT
                     GLHALGWPRDRAIAFMLEHTALGPNNITNEVDRYIVLPGQALAYKLGQIEMLRLRAEA
                     RERLGDRFDIRGFHDAMLGSGAVTLPILRGVVEAWATGRQASA"
BASE COUNT          437 a         1091 c         1113 g          346 t
ORIGIN      
        1 gcagcagcgc gatctccccg atcccggccc cggcgccctc acgatggtgt ggttgtccgt
       61 ccatcgcgac ctccacgtcg ccgcgctcga tcacgacgaa cgtgtcgcct tcctcgccct
      121 gcgacatgag ccgctcgccc gcggcgaagc gcacctcgcg catcccggtc gcgacgcgct
      181 ccagcgccgc cagcgggagc atcgcgaaca gcgggatccc gcgcagcagc gacgcctggc
      241 gctcgggcac gacgccctcg gtgtcgagcc gccggacgag cggccagccc aggaccgcga
      301 caaccggcag gctgaggccc acgaggacga gggcccgctc gatccccagc gttccgatca
      361 gcagcgaagc gacgatggcg ccgatggaca cgctggcgct ggcgacgacc tcgaggaccg
      421 cgaagaccgc cgaccgggcg cggttcgagg tgccgcgctg gaggagcgtg agccccgaca
      481 cgtcgatcag cgcgttgccc acgcccacga cgccgagcgc gagcagcgcc acgaccgtca
      541 cgggaacgaa gccgatgacg gcgatcgggg caccccacag gacgagggcg atcgcgaaca
      601 cgacggccag ccgacgctcc gcaccgatcg cgagcgcgcc gagcgcgccg gcgatcccgc
      661 ccaggccgat cgccgcaccc agcacgccga caccggcgtc gcccatgccc aggacctcga
      721 tcgcgaggat ggcgaggaag gtcgtcagcg caccgcgcac ggccgtctgg gcgatgaacg
      781 acaccatgac catgccggcg ggcggccgcg cgacgagctc gcgcacgccg gcgaccaacg
      841 ggatggacgc ccggcgctcc cgatcgcgca gccgcgaggc gtcgggcacc cggacggccg
      901 cggccacgcc cgccgcgacg aggcacagca cgcccgagac cacggccgcc ggcgccgcgc
      961 caccctgcgc gatgacgagg cctgccacca gcggcccggc aaacgtgccg aggctctcgc
     1021 ccagggcgcc cgccgtgttc gcgctgacga gctcggcggg gctcgtcgcg actgcgggca
     1081 gcagcgtcag gaccgtcggc cgcaccagcg cgcccgccgc cgacgcggcc gcgaccgcca
     1141 cgaacaccag ggccgtggcg tcggcgaggc tggccgcggc gacgacgacc gccccggccg
     1201 cgcggacgag gttggcgccg atcagcgcgc gctcgggcct ggggaacgcg ctcgtgtcga
     1261 tgacgacgtt gacgacggtg gcggggatca tgcgggtgag cgagaccagc ccgaccagga
     1321 cggcgccacc ctcgtcgtac gcgacgagca gcgcgacgac cagcagcgcc cagtccgcgg
     1381 ccacacccag cgtccaggcg agctcgagcg agcggatatc gcggttgtgc aggacgccga
     1441 gcagcgaccg gccggcgagc gcaagcgggg ttctcgccgg cgggtcgctg ggctcccccg
     1501 tcatcaggcc gaggcttggc gacccgtggc ccaggcctcg acgaccccgc gcaggatcgg
     1561 cagcgtgacc gcgccgctcc ccagcatcgc gtcgtggaag ccgcggatgt cgaaccggtc
     1621 gcccagccgc tcgcgggcct ccgcccggag acgcagcatc tcgatctggc cgagcttgta
     1681 ggcgagcgcc tggcccggca gcacgatgta gcggtcgacc tcgttggtga tgttgttggg
     1741 tccgagcgcc gtgtgctcga gcatgaacgc gatggcacgg tcccgcggcc agccgagggc
     1801 gtggaggccg gtgtcgacga cgaggcgcgc cgcccgccag gcgtcgaacg agaggacgcc
     1861 gatccggtcg aggtcgccgc tgtagagccc catctcgtcg ctcaggcgct ccgtgtacag
     1921 gccccagccc tcgaagaagg cggtcggtcc gaggtgccgg cggaactcgg gaatgtgcgg
     1981 cagctcctgg ccgatcgcga tctggagatg gtgccccggg accgcctcgt ggtaggcgag
     2041 cgactcggcc tcgtaccgcg gcctggtcgt cggggcggac gtgttgatgt agtagctgcc
     2101 cggccgcgac ccgtccgtcg ccggctggcg gtagtacgcg atcgtcgagt gctcctcctc
     2161 gcggggaccc atgcggacga cctcgcatgg cgcctcgggc agtcgcccga accagtcggg
     2221 gatggcctcg gtggcgcggg cgaggcacca ggccgccttg gcgaacacct cgtcccgggt
     2281 cgcgaagtgg agggcagggt cgctgcgcag gtgcgacagc gcgtcgccca ggctcgacgt
     2341 gccgatcgtg cggccggcga ggtccgtgag ctcggcgtcg atccgcgcga tctcgtcgag
     2401 gccgacccgg tggatctccg cggcatcgag gtcgagcgag gtgtgcacgc ggatcagctc
     2461 gcgataggcg tcgggccgac ccggcacctc gcccatgccc ggccgctccg ccgggcgggc
     2521 acgtggcagg atctcgtcga cgagcgcgtc gcgaaggcgg acgaaggcgg gtcggatctc
     2581 gtcctctacg gccctctgca ggctctcagc gaatcgctcc cggtccgagg ttgaccagcc
     2641 gggctggtcg gtgaccgtgc gcagcggcgc cagcagcggc caggccgcgg tgtcgtcgcg
     2701 caggagatcg tcgaggattg cgatcgttcg ctcggtcggc gggcgggacg cgatccggcc
     2761 gccggcgagg ctccggcgga ggttgcccag gtggcggtcg gtgtgggcgg ccatcgcccg
     2821 ccagcgggcg accatgcgca gaccgtcctc gggtgtgtcg agccgctggt agtcggggac
     2881 gagcaggaac atcgcgggca cgccctcgat gggatcgacg ttccagtcga gcaggcccgt
     2941 gtcgagctcg gcgatgtccg acgcgatcga ctcgcgcagc atcgaca
//
LOCUS       DBNHXO010000173         2393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3614126, whole genome shotgun sequence.
ACCESSION   DBNHXO010000173 DBNHXO010000000
VERSION     DBNHXO010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3614126"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /gene="lepB"
                     /locus_tag="AC162A_03705"
     CDS             <1..240
                     /gene="lepB"
                     /locus_tag="AC162A_03705"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR5009974.1"
                     /translation="WVVPAGQLFVMGDHRQRSADSRVFGPISTSDVIGRAFLRYWPIS
                     TFGILQTPSYPGVPSATGVAPKPGSSDPALQSAAP"
     gene            263..1003
                     /locus_tag="AC162A_03710"
     CDS             263..1003
                     /locus_tag="AC162A_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009975.1"
                     /translation="MTPLLVALAGLVAVGGVVAVSAREPRFAVLGVIIALVGAGYVAD
                     PLPSLAALAARLTGAVLGGYLIWVALRRAPAQTAGWHVGWPGAVALAAAAFAAGWFAA
                     GSLAAALSTTAGEGPSAAGVAAALTAGSPVARAALAAAFALLALAAAPVLVARDVLRL
                     GIGLLLLLAAAGLVRNALAPGDDAAIELGFGIIEALTGAAIAALVAASIRLHYDLELR
                     GSGARQPAIHHRSTDEAHLGRRAPADER"
     gene            1000..>2393
                     /locus_tag="AC162A_03715"
     CDS             1000..>2393
                     /locus_tag="AC162A_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
                     /protein_id="HIR5009976.1"
                     /translation="MTLLVLIATGSVGAGVAAWGLGRGGRPAQIGALVGWLSLIAVLV
                     LALAMEAPRVGSGAPAAGLAAFNGHLVPNDYLRLVVGLWALDAVLFVAIGWLLGGLDG
                     LRGLLPATLAAIVGGTVAFAATDLTLGSIAAGAVGLSALAVLLVGRDAVTAAARELRV
                     AIAGTAILIAVAAAAPAAATLVFDALGASPDTGQAPTTAPTEAGAMIGLLAVAVALVV
                     AVRLGSIPFHLRVPRLADVAPPISLPLLLAWIPVPLGIAGIAITDRMLAPLALPLEGE
                     QALIVGIALLTLVAGALAAFIQDDLRHATGYLVVADGGLVLLGFAALDPAAWGPTRVW
                     LVAMAASKTAFAAWAAVMEDRFETRAIPDLRGWLRHSPIMGAALIVTVIATYGLPGWV
                     AFEARIDLAQLSGSSITWLIVIAGLATLPTYTRLLAVGLGPATSRVRKAAPERVTRAR
                     RTDTLPVTLEGGPAT"
BASE COUNT          220 a          928 c          886 g          359 t
ORIGIN      
        1 tgggtggtcc cggcggggca gctgttcgtc atgggcgacc accgccagcg atcggccgac
       61 tcgcgggtgt tcggcccgat cagcacgtcg gacgtgatcg gtcgggcatt cctgcgctac
      121 tggccgatct cgaccttcgg catcctccag accccctcgt acccgggcgt gccgagcgcc
      181 acgggcgttg cgccgaagcc agggtcgtcc gaccccgccc tccagtccgc ggcaccctga
      241 cgccccgggc cgagccgatg ccgtgacgcc cctgctggtc gcactcgccg ggctcgtggc
      301 cgtcggcggc gtcgtcgccg tcagcgcccg tgagccacgg ttcgccgtgc tgggcgtgat
      361 catcgcgctg gtcggcgccg gctacgttgc ggacccgctg cccagcctcg ccgcgctcgc
      421 cgctcgcctc accggcgccg tgctcggcgg ctacctcatc tgggtcgcgc tccgacgcgc
      481 gcccgcgcag accgcaggct ggcacgtcgg ctggccgggc gccgtggcgc tcgctgccgc
      541 ggcgttcgca gcgggctggt tcgccgcggg atcgctcgcg gcggcgctga gcacgaccgc
      601 cggcgagggc ccgtcggcgg cgggcgtcgc cgccgcgctg accgccggtt cgcccgtggc
      661 ccgtgcagcg ctggccgcgg cgttcgcgct gctcgcgctg gccgcggcgc cggtcctcgt
      721 cgcgcgcgac gtgctgcgcc tcggcatcgg cctgctgctc ctgctcgccg ccgccggcct
      781 ggtgcgcaac gccctcgcac cgggcgacga cgcggcgatc gagctcgggt tcgggatcat
      841 cgaggcccta acgggcgccg cgatcgccgc gctcgtggcg gcctcgatcc ggctccacta
      901 cgacctcgag ctccgcggct cgggcgcgcg ccagccggcc atccatcatc gatcgaccga
      961 cgaggcgcac ctcggacgtc gggccccggc cgacgagcga tgaccctgct cgtgttgatc
     1021 gcgaccggat cggtcggcgc cggcgtcgcc gcatgggggc tgggtcgggg tgggcgaccc
     1081 gcccagatcg gtgcgctcgt cggctggctg agcctcattg cggtgctggt gctggcgctc
     1141 gcgatggagg ccccgcgcgt cgggtcggga gcgcccgccg ccggcctggc ggcgttcaac
     1201 ggccacctcg tcccgaacga ctatctccgg ctcgtggtcg gcctgtgggc gctcgacgcg
     1261 gtcctgttcg tcgcgatcgg ctggctcctg ggcggcctcg acggcctccg cggcctgctg
     1321 cccgccacgc tcgcggccat cgtgggcggg acggtcgcgt tcgccgcgac ggacctgacg
     1381 ctcggatcga tcgcggcggg ggcggtgggg ctgtcagcgc tggccgtgtt gctcgtgggc
     1441 cgggacgcgg tgacggcggc ggcccgcgag ctccgggtcg cgatcgccgg gaccgcgatc
     1501 ctgatcgcgg tcgccgcggc ggcgccggcg gcggcgaccc tcgtgttcga cgccctgggt
     1561 gcgagtccag acaccggcca ggcaccgacg accgcgccca ccgaggcggg cgcaatgatc
     1621 gggctgctcg cggtcgccgt ggccctcgtc gtcgcggtcc ggctgggctc gatccccttc
     1681 catctccgcg tcccccggct ggccgacgtc gcgccgccga tctcgctgcc cctgctgctc
     1741 gcctggatcc cggtccccct cggcatcgcc ggcatcgcga tcacggaccg gatgctcgcg
     1801 ccgctcgccc tgcccctcga gggcgagcag gcgctcatcg tgggcatcgc gctgctgacg
     1861 ctcgtcgccg gagccctcgc cgccttcatc caggacgacc tgcgccacgc cacgggctac
     1921 ctcgtggtcg ccgacggcgg cctcgtgctg ctgggcttcg cggccctgga tcccgcggcc
     1981 tggggtccga ctcgcgtctg gctggtggcc atggccgcgt cgaagacggc gtttgcggcg
     2041 tgggcggcgg tgatggagga ccggttcgag acgcgcgcga tcccggacct ccgcggctgg
     2101 ctgcgccact ccccgatcat gggcgcggcg ctcatcgtca cggtcatcgc gacctacggg
     2161 ctgccgggct gggtcgcgtt cgaggctcgg atcgacctcg cgcagctgtc cggcagcagt
     2221 atcacctggc tgatcgtcat tgccggcctc gcgacgctgc cgacgtacac gcggctgctc
     2281 gcggtaggcc tcgggccggc gaccagccgc gtgcggaagg cggcccccga acgggtgaca
     2341 cgcgcccgcc gcaccgacac gctccccgtg acgctcgagg gtggtccggc gac
//
LOCUS       DBNHXO010000174         2134 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1036507, whole genome shotgun sequence.
ACCESSION   DBNHXO010000174 DBNHXO010000000
VERSION     DBNHXO010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1036507"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..842
                     /locus_tag="AC162A_03720"
     CDS             117..842
                     /locus_tag="AC162A_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5009977.1"
                     /translation="MIGTRPLRILVVDDDPAMVGAITALVGTEGHQVITAYDGLTAVK
                     RYREERPDLVLLDLAMPGPDGFTVIGQLRATGNVPVLVVSGESAEAAKVKALGIGADD
                     YLVKPFGKQELLARISAVMRRVDLAGTGRIGGNGDGPAGVLTAGSLTLEPARHEARVG
                     AIALNLTPTEFRLLEALVRAGGDLVAHHRLARAGWPAEPDPDLLWLKPHLARLRSKLE
                     AVGGPRIVAVRSVGYRIEADEPA"
     gene            997..>2134
                     /locus_tag="AC162A_03725"
     CDS             997..>2134
                     /locus_tag="AC162A_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5009978.1"
                     /translation="MLTRSRFAALVALALVLVAAAPAGAATPSARIAADAAAYDGRLI
                     VVWKGAAPKSLRMPGVASTERTSRPMRSVVTTLPGRTALVAAALRANPDVLVVVPDAR
                     MSLLDWPDDGSPSDPLFAGQKDLEQIGVPDAWPTTTGDPSVVVAVIDSGVDLTHPDLD
                     GVSVVAPRNVVYNSSDVTDGVGHGTHVVGTILAETDNGVGVAGIAPGSRLMPVKVADA
                     DGFLSFSDILDAVDWARENGADIINMSLGGQLSPEQVALAQPTFTTARAAGILMVAAS
                     GNDGVPFRMYPASFNGVVSVSAVDKHDKIADFSNTGRAVDIAAPGVDLISTVPDGGYE
                     RYSGTSMASPHVAGVAALTWAARPGLAVDELEAVLRASAVDLGDP"
BASE COUNT          296 a          796 c          746 g          296 t
ORIGIN      
        1 ctgtggtgcg agccggcgcc atccggtggc gcgaggttcg tgctcgcgct ccccgccgcc
       61 gtggcggtct gacatgacac ccaacgacga ttgacggaag gctgggcgag acgacgatga
      121 tcggcacgag accgctacgg atcctggtgg tggacgacga cccggcgatg gtcggcgcga
      181 tcaccgcgct ggtcggcacc gagggccacc aggtgatcac cgcatacgac ggcctcaccg
      241 ccgtgaagcg gtaccgcgag gagcggccgg acctcgtcct gctcgacctc gcgatgcccg
      301 ggcccgacgg gttcacggtg atcggccagc tgcgggcgac cggcaacgtc cccgtcctcg
      361 tcgtgtccgg cgagagcgcc gaggcggcca aggtcaaggc gctgggaatc ggcgccgacg
      421 actacctcgt gaagccgttc ggcaagcagg agctgctcgc gcggatctcg gccgtcatgc
      481 gccgggtcga cctggccggc acgggccgca tcggcggtaa cggcgacggc cccgcggggg
      541 tcctgaccgc cggctcgctg accctggagc ccgcccgcca cgaggcgcgc gtcggggcca
      601 tcgcgctcaa tctgacgccg accgagttcc ggctgctcga ggcgctggtc cgcgcgggtg
      661 gcgacctcgt cgcgcaccac aggctggccc gcgcaggctg gccggccgag cccgacccgg
      721 acctcctgtg gctcaagcca catctcgccc ggctgcgctc caagctcgag gccgtcgggg
      781 gcccgcggat cgtcgccgtg cgctccgtgg gctaccggat cgaggccgac gagcccgcct
      841 gacagcgacc cggaaagacg ttcggaaaat cgggctgact ggcccatagc gccttcacgg
      901 gcctagggct acactcccgc tggcccgctc gccgtgaggc cgatcccgtc atgacgggcc
      961 acattgtcag gccgaagaat cgaaggggaa agccgggtgc tcacccgctc gcgtttcgcg
     1021 gcgctcgtcg cgctggcgct cgtcctcgtc gcggcagccc ccgcaggcgc cgctacccct
     1081 tcggcgcgca ttgccgccga cgccgcggca tacgacggcc gcctgatcgt cgtctggaag
     1141 ggcgccgcgc cgaagtcgct ccggatgccg ggcgtcgcct ccaccgagcg cacctcgcgc
     1201 ccgatgcgat cggtcgtcac gacgctgccg ggacgaacgg cgctggtcgc cgcggccctg
     1261 cgggccaacc cggacgtgct ggtcgtcgtg cccgacgcca ggatgtccct gctcgactgg
     1321 cccgacgacg ggagcccgtc cgacccgttg ttcgcgggcc agaaggacct cgagcagatc
     1381 ggcgtgccgg atgcctggcc gacgaccacc ggcgacccgt cggtcgtcgt cgctgtcatc
     1441 gactccggcg tcgacctcac ccacccggac ctcgacgggg tctcggtcgt cgcgccccgc
     1501 aacgtcgtct acaactcgag cgacgtgacc gacggggtcg gccacggcac gcacgtcgtg
     1561 ggcacgatcc tcgccgagac cgacaacggc gtcggcgttg cgggcatcgc ccccggttcc
     1621 aggctgatgc cggtcaaggt cgccgatgcc gatgggttcc tgtccttctc ggacatcctc
     1681 gacgccgtcg actgggcgcg cgagaacggc gccgacatca tcaacatgag cctcggcggc
     1741 cagctgtcgc ccgagcaggt tgcgcttgcg cagccgacct tcacgacggc gcgagcggcg
     1801 gggatcctga tggtcgccgc gtccggcaac gacggcgtcc cgttccggat gtacccggcg
     1861 tcgttcaacg gcgtcgtgtc ggtgagcgcc gtcgacaagc acgacaagat cgccgacttc
     1921 tcgaacaccg ggcgcgcggt agacatcgcc gcccccggcg tcgacctgat ctccaccgtc
     1981 cccgacggcg gctacgagcg ttactcgggc acgagcatgg ccagcccgca cgtcgcaggg
     2041 gtcgcggcgc tcacctgggc ggctcgcccc ggcctcgcgg tcgacgagct cgaggccgtg
     2101 ctgcgcgcga gcgccgtcga tctcggcgat cccg
//
LOCUS       DBNHXO010000175         1586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2424749, whole genome shotgun sequence.
ACCESSION   DBNHXO010000175 DBNHXO010000000
VERSION     DBNHXO010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2424749"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="AC162A_03730"
     CDS             complement(<1..164)
                     /locus_tag="AC162A_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HIR5009979.1"
                     /translation="MATATSRRTRLDLLLVNRGLAESRARAQALVMAGQVRVGDGDSA
                     RTDLKAGDLVA"
     gene            complement(231..>1586)
                     /locus_tag="AC162A_03735"
     CDS             complement(231..>1586)
                     /locus_tag="AC162A_03735"
                     /inference="COORDINATES: protein motif:HMM:NF014798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR5009980.1"
                     /translation="HDRPLLEETFRRALALPGPVIVHVRTQKGKGFRPAETDQISFHG
                     AALPPITVPEAVDAHERMGTGQINGDPMASAAAAGSQVTTGRQAEAGAPPSSSVMADR
                     RTPAHNSEGAAQPPAAQASDVVKTPNYTAFFARELVELGATDRRIVAITAGMPTGTGL
                     AKFQAQYPERFIDVGIAEQHAVTLATGLALGGMRPVVALYSTFLQRAFDQTVHDVCQN
                     DAPVLLAVDRAGLVGEDGTSHQGMFTIPAQRMLPNLVVASPRDEQELRSLLRTALAQD
                     HPFALHYPRDAGFGVPEREAEVLPVGRGEVLREGRNLLFVTFGPITGRAIQVADSLEE
                     RGWSVGIINARFVKPLDRQLILDQARGKSLVVTFEESVVTGGFGSAVLEAFEEARLAD
                     PSYRSIPVRTIGIPGDRFVDHGSVTDLRRLIRLDVPGLTAQVDETLAQLGLEVPAAKH
                     A"
BASE COUNT          212 a          571 c          586 g          217 t
ORIGIN      
        1 gccaccaggt cgccggcctt gaggtccgtg cgcgcgctgt cgccgtcgcc cacccggacc
       61 tggcccgcca tcacgagcgc ctgggcccgg gcgcgcgatt ccgcgagccc gcggttcacg
      121 agcaggaggt cgagtcgtgt tcgacgcgag gtcgccgtcg ccatctgcct atcgtggcat
      181 acgggccctt acctgcggcg cacccgctgc cgccgggcgt cgccaaagcg tcaggcgtgc
      241 ttcgcggccg gtacctcgag gcccagctgg gcgagcgtct cgtcgacctg cgccgtgagg
      301 cccggcacgt cgaggcggat caggcgccgc aggtcggtca ccgagccgtg gtcgacgaag
      361 cggtcgcccg ggatcccgat cgtgcgcacc gggatcgagc ggtacgacgg gtcggccagc
      421 cgcgcctcct cgaacgcctc gagcaccgcg gacccgaagc cacccgtcac gacgctctcc
      481 tcgaacgtca ccacgagcga cttgccacgc gcctgatcga ggatcagctg gcggtcgagc
      541 ggcttcacga accgggcgtt gatgatcccg accgaccagc cgcgctcctc gagcgaatcg
      601 gcgacctgga tcgcccgacc ggtgatcggg ccgaaggtga cgaacaggag gttgcggccc
      661 tcgcgcagga cctcgccccg gccgaccggc agcacctcgg cctcgcgctc cgggacgccg
      721 aacccggcgt cacgcgggta gtgcagcgcg aacgggtggt cctgggccag ggccgtccgg
      781 agcagcgagc ggagctcctg ctcgtcgcgc gggctggcga ccacgaggtt gggcagcatc
      841 cgctgggcgg gaatcgtgaa catgccctgg tggctggtgc cgtcctcccc cacgagcccc
      901 gcccggtcga cggcgagcaa caccggggca tcgttctggc acacgtcgtg gacggtctgg
      961 tcgaacgccc gttgcaggaa cgtcgagtac agcgcgacga ccggccgcat gccgcccagc
     1021 gcgaggcccg tcgccagggt gaccgcgtgc tgctccgcga tcccgacatc gatgaagcgc
     1081 tccgggtact gcgcctggaa cttggcgagg ccggtgccgg tcggcatgcc cgcggtgatc
     1141 gccacgatcc ggcggtccgt cgcgccgagc tcgaccagct cgcgggcgaa gaaggctgtg
     1201 tagttcggcg tcttgacgac gtcggaggcc tgggctgccg gcggctgggc ggcgccctcg
     1261 gagttgtgcg cgggcgtccg tcgatcggcc atcacggacg acgagggcgg cgccccggcc
     1321 tccgcctggc ggccggtcgt cacctgcgag ccagccgcgg ctgccgaggc catcgggtcg
     1381 ccgttgatct ggccggtgcc catgcgctcg tgcgcgtcga ccgcctcggg gacggtgatc
     1441 ggcggcagcg cagccccgtg gaacgagatc tggtcggtct ccgcgggccg gaaacccttc
     1501 cccttctggg tgcggacgtg gacgatcacc gggccgggaa gggcgagggc gcgacggaag
     1561 gtctcctcga gcagcggccg gtcgtg
//
LOCUS       DBNHXO010000176         2712 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3019687, whole genome shotgun sequence.
ACCESSION   DBNHXO010000176 DBNHXO010000000
VERSION     DBNHXO010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3019687"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1099
                     /locus_tag="AC162A_03740"
     CDS             74..1099
                     /locus_tag="AC162A_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5009981.1"
                     /translation="MTTTTLQARGGFVDSLRNMRAGPYLFVLAVALIVYVVIGPQQFI
                     NALTIGAIYALVALGYTMVYGIIELINFAHGDVFMVGAFASMFVLTSVLGQEGSATSV
                     PQAVFLIVTTFLIVMTMMGLLGVAIERFAYRPLRNAPRLAPLITAIGVSFILQNVIQI
                     LYGPSPINTPQVIPPTAKIQIGNVTIGLINVFVITVSVGLMIGLQLFVNRTRLGRAMR
                     STAQDRDAAQLMGVDINRTIALTFFIGSALAGAAGVVQGLYFGNIQFNLGFQAGLKAF
                     TAAVLGGIGNITGAALGGFVIGFVEVTAAALGLFAWGQALVFAVLIITLVFRPSGLLG
                     QQLGERA"
     gene            1096..2265
                     /locus_tag="AC162A_03745"
     CDS             1096..2265
                     /locus_tag="AC162A_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5009982.1"
                     /translation="MTAEIRETTRGTGGAGAPWRQRVGVFSDNHRSTSVLIGMVIVTS
                     LLPLIVLLPPFSSFNVQNVWLDGFTIAGIYVLLALGLNIVVGLSGLLDLGYAAFFAIG
                     SYTFAYAASPFTGFDFPFWPMLLVGALTAAIFGVLLGAPTLRLRGDYLAIVTLGFGEI
                     VPIVILNSSKYTEGPNGIGGIPPPAIGDFTFPSVGNPWPFYITMVALITISMMIIYRL
                     QDSRLGRAWMAIREDELAASANGINTVTTKLLAFALGASTAGLAGVFASSKLTLVSPN
                     LFRFDVSFTVLAMVVLGGMGNIWGVAIGAFIIFMIQTVILKQLNIFFDNVEVPILSDI
                     DFIQYQFLLYGIALVAMMLLRPEGLFPSRRRKRELHTDEDADFGEMGDPLGASPE"
     gene            2277..>2712
                     /locus_tag="AC162A_03750"
     CDS             2277..>2712
                     /locus_tag="AC162A_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5009983.1"
                     /translation="MTDSNSQGPAAPEADQDILLRADRVSRRFGGLIAVRDVDLVIPR
                     GGIVSIIGPNGAGKTTFFNVIAGIIEPTAGTVSLRGQVLITRPRRAWLESVLWVLPPA
                     VVLLVGMLLGASNSEAGLGITGLVAVAVLVTILVAALARPPWY"
BASE COUNT          377 a          977 c          849 g          509 t
ORIGIN      
        1 ttcatcgggc cggggggcaa ccgccccccg gcccgtgtct tccccggtcc ctgacgatca
       61 gctcggaggc cgcatgacca cgacgaccct gcaggcacgc ggtggtttcg ttgattccct
      121 gcgcaacatg cgggctgggc cctacctgtt cgtgctggcc gtcgcgctga tcgtgtacgt
      181 ggtcatcggg ccacagcagt tcatcaacgc actgaccatc ggcgccatct atgcgctcgt
      241 cgcgctcggc tacacgatgg tctacggcat catcgagctc atcaacttcg cccacggcga
      301 cgtgttcatg gtcggggcct tcgcctcgat gttcgtgctc accagcgtcc tcggccagga
      361 aggctccgcg acatcggtcc cccaggccgt gttcctgatc gtcaccacgt tcctgatcgt
      421 gatgacgatg atgggcctcc tcggcgtggc catcgagcgg ttcgcctatc gcccgctccg
      481 caatgccccc cgtctcgccc ccctgatcac ggcgatcggc gtgtcgttca tcctgcagaa
      541 cgtcatccag atcctgtacg gcccgtcccc gatcaacacg ccccaggtca tcccgcccac
      601 ggccaagatc cagatcggca acgtgaccat cgggctgatc aacgtcttcg tgatcaccgt
      661 gtcggtcgga ctgatgatcg ggctgcagct gttcgtgaac cggacgcgcc tgggtcgcgc
      721 gatgcgctcg acggcccagg accgggacgc ggcccagctg atgggcgtcg acatcaaccg
      781 cacgatcgcc ctgacgttct tcatcggatc agcgctcgct ggtgcggcgg gcgtcgtcca
      841 gggcctctac ttcggcaaca tccagttcaa cctcgggttc caggccgggc tcaaggcgtt
      901 caccgccgcg gttctgggcg gcatcggcaa catcaccggc gcggccctcg gcggcttcgt
      961 gatcggcttc gtggaggtca ccgcggccgc gctcgggctg ttcgcatggg gccaggcgct
     1021 ggtgttcgcg gtgctgatca tcaccctcgt gttccggcca tccggcctgc tcggccaaca
     1081 gctgggagaa cgggcatgac ggcggagatc cgcgagacga cgcgcgggac cggcggcgcc
     1141 ggggcgccgt ggcgtcagcg ggtgggcgtc ttctccgaca accaccgctc gacgagcgtg
     1201 ctgatcggga tggtgatcgt cacctcgctc ctgccgctga tcgtgctcct gccgccgttc
     1261 agcagcttca acgtccagaa cgtgtggctc gacgggttca cgatcgccgg catctacgtc
     1321 ctgctggccc tgggcctgaa cattgtcgtt ggcctgtcgg gcctgctgga cctcgggtat
     1381 gcggcgttct tcgccatcgg gtcgtacacc ttcgcgtacg cggcctcgcc gttcaccggc
     1441 ttcgacttcc cgttctggcc gatgctgctc gtgggcgcgc tgaccgccgc catcttcggg
     1501 gtgctgctcg gcgccccgac cctgcgcctg cgcggcgact acctcgcgat cgtgaccctg
     1561 gggttcggcg agatcgtgcc gatcgtcatc ctgaactcgt ccaagtacac cgaggggccc
     1621 aacggaatcg gcggcatccc gccgcccgcg atcggcgact tcaccttccc gagcgtcggc
     1681 aacccgtggc cgttctacat cacgatggtc gccctgatca cgatctcgat gatgatcatc
     1741 taccggctcc aggactcccg cctcggccgc gcctggatgg cgatccgcga agacgagctc
     1801 gcggcgtcgg ccaacggcat caacaccgtc acgaccaagc tcctcgcgtt cgccctgggc
     1861 gcgtcgaccg ccggcctcgc gggggtgttc gcgtcgtcca agctcaccct ggtcagcccg
     1921 aacctgttcc ggttcgacgt ctcgttcacg gtgctcgcga tggtggtcct cgggggcatg
     1981 ggcaacatct ggggcgtcgc gatcggtgcg ttcatcatct tcatgattca gacggtgatc
     2041 ctgaagcagc tcaacatctt cttcgacaac gtcgaggtgc cgatcctcag cgacatcgac
     2101 ttcatccaat atcagttcct gctctacggc atcgccctgg tcgcgatgat gctgctgcgg
     2161 cccgagggac tcttccccag ccgccgccgc aagcgcgagc tgcacaccga tgaggacgcc
     2221 gacttcggcg agatgggcga cccgctcggg gcgtcacccg aatgagcgcc gagcccgtga
     2281 ccgacagcaa cagccagggg ccggctgcgc ccgaagccga ccaggacatc ctgctccgcg
     2341 ccgaccgggt gagtcgccgc ttcggcggcc tgatcgccgt ccgcgacgtc gatctcgtca
     2401 ttccgcgcgg cggcatcgtc agcatcatcg gcccgaacgg cgccggcaag acgaccttct
     2461 tcaacgtgat cgccgggatc atcgagccga ccgccgggac cgtgtcgctc cgcgggcagg
     2521 tcctcatcac ccgcccccgc cgggcttggc tcgagtccgt cctgtgggtc ctcccgcctg
     2581 ctgtcgtcct gctcgtcggc atgctgctcg gcgcgtcgaa cagcgaggct ggattgggga
     2641 tcaccggcct cgtggcggtc gccgtgctgg tgacgatcct cgtcgccgcg ctggcccgcc
     2701 cgccgtggta cc
//
LOCUS       DBNHXO010000177         2826 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5203224, whole genome shotgun sequence.
ACCESSION   DBNHXO010000177 DBNHXO010000000
VERSION     DBNHXO010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5203224"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="AC162A_03755"
     CDS             complement(<1..259)
                     /locus_tag="AC162A_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR5009984.1"
                     /translation="MTSRFASDLAFARETAERAGEILFERYERVGRIDHKSARDVVTE
                     VDHLSEALVLDAIRSRFPGDGILAEESGAHRTTTADATRGLA"
     gene            complement(256..741)
                     /locus_tag="AC162A_03760"
     CDS             complement(256..741)
                     /locus_tag="AC162A_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009985.1"
                     /translation="MPTTPTPAAAPAPAEPMHGCVRCGARIPISEAMCEQCNPLGLKQ
                     PSASQAHGTAILGVAIAVVVLAVIARLAISGVGPFTSSLVGFAADPAGIRLTISVTNG
                     GTSAAATTCRIGDPELRGIGPETAFVQSPIIQPGATATFDRVVTSLGTEPRPLTVDCS
                     R"
     gene            complement(762..1610)
                     /locus_tag="AC162A_03765"
     CDS             complement(762..1610)
                     /locus_tag="AC162A_03765"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006605069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HIR5009986.1"
                     /translation="MTDLAEIGVFGGSGFYTLLEDVREAKVDTPYGPPSDSVFLATIA
                     GRRVAFLPRHGRRHTIPPHRINYRANVWAMRSLGVRAVISPCAAGSLQLAVEPGHFVV
                     SDQFVDRTTGRADTFFDGPIVSHVSSAEIYDPTLRQIAIDVIREHGITVHDGGTVVVI
                     QGPRFSTKAESKWFSDAGWEVINMTQYPEAYLCRELGMAVVNIALITDYDAGVLEGTH
                     AVTAHDVLAVFEQNAERIRKVVLDMVGRFPADLDTLGARDALAHTRGDGHVAGDADIR
                     IFETGL"
     gene            1694..2122
                     /locus_tag="AC162A_03770"
     CDS             1694..2122
                     /locus_tag="AC162A_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR5009987.1"
                     /translation="MPTTAALSDAVRTFLDQPNVASVATTDDDGQPRQAVVWYRLEPD
                     GRILINSRTPRRWCANLVRDPRLAISIIDAEDAYRWIGLTGVVEEVVDDVSRSREDIV
                     ALAYRYRPEGPTEASIAMFRSQPRVTFLARITGIHDHLED"
     gene            2126..>2826
                     /locus_tag="AC162A_03775"
     CDS             2126..>2826
                     /locus_tag="AC162A_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR5009988.1"
                     /translation="MASVKLGVLLWPQTRSWPDIRDAAALADRSGAASLWTWDHLNSI
                     VGPWEGPIFEGWSILAGWSQVTERATLGLMVAANTFRNPGLTAKLATTLDHLSGGRAI
                     LGLGGAWFEREHEAYGIDFGSGFGERLDRLDEAVMLVRRLLDGETVTHDGPVYPMRDA
                     LVTPRPVQVRLPIRIGGSGPKQTLRTLARYGDQWNAMGELPKRAERDAILREHCATFG
                     RDDAEIERTTTVDIV"
BASE COUNT          406 a         1017 c         1006 g          397 t
ORIGIN      
        1 cggcgagccc gcgcgtcgcg tcggccgtcg tggtccggtg tgccccggac tcctcggcca
       61 ggatcccgtc acccggaaag cgcgagcgga tcgcgtcgag gacgagcgcc tcggacaggt
      121 ggtcgacctc ggtcaccacg tcccgcgccg acttgtggtc gatccggccg acccgctcgt
      181 agcgctcgaa caggatctcg cccgcccgct ccgccgtctc acgcgcgaac gcgaggtcgg
      241 acgcaaaccg gctggtcacc gtgagcagtc gacggtcagc ggccgcggct ccgtcccgag
      301 gctggtcacg acccggtcga acgtcgcggt cgcgccgggc tggatgatcg ggctctggac
      361 gaaggccgtc tcgggtccga tgccgcgcag ctccgggtcc ccgatccggc aggtcgtcgc
      421 ggcggcgctg gtcccgccgt tggtcaccga gatcgtcagc cggatccccg cggggtccgc
      481 ggcgaacccg accaggctcg acgtgaacgg cccgacgccg ctgatcgcga gccgcgcgat
      541 caccgccagc acgacgaccg cgatcgcgac gcccaggatg gccgtgccat gggcctggga
      601 cgccgagggc tgcttgaggc cgagcgggtt gcactgctca cacatcgcct cggagatcgg
      661 gatccgggcg ccgcaccgca cgcagccgtg catcggctcc gcgggggcgg gcgctgcggc
      721 aggcgtcggg gtcgtcggca tggaggtcat ccgggcgacg gctagagccc cgtctcgaag
      781 atccggatgt cggcatcgcc ggcgacgtgg ccgtcgccgc gcgtgtgggc gagggcatcg
      841 cgcgccccga gcgtgtcgag gtcagcggga aaccggccga ccatgtccag cacgaccttc
      901 cggatccgct cggcgttctg ctcgaacacc gcgagcacgt cgtgcgcggt gacggcgtgc
      961 gtgccctcga gcacgcccgc gtcgtagtcg gtgatcagcg cgatgttgac gaccgccatc
     1021 ccgagctcgc ggcacaggta cgcctcggga tactgggtca tgttgatgac ctcccacccg
     1081 gcgtcgctga accacttcga ctcggccttg gtggagaacc ggggaccctg gatcacgacc
     1141 accgtgccgc cgtcatggac ggtgatcccg tgctctcgga tcacgtcgat ggcgatctgg
     1201 cggagcgtgg ggtcgtagat ctccgcggac gagacgtggg acacgatcgg gccgtcgaag
     1261 aacgtgtccg cgcggccggt cgtgcggtcg acgaactggt cgctgacgac gaagtggccg
     1321 ggctcgacgg cgagctggag ggatcccgcc gcgcacgggc tgatgacggc cctgacgccc
     1381 agcgagcgca tcgcccacac gttcgcccgg tagttgatcc ggtgaggcgg gatcgtgtgc
     1441 cgccgcccgt ggcgcggcag gaacgccacc cgccggcccg cgatcgtcgc caggaacacg
     1501 ctgtcggacg gcgggccgta gggcgtgtcg accttggcct cgcgaacgtc ctcgagcaac
     1561 gtgtagaacc cggaaccgcc gaagacaccg atctccgcga ggtccgtcac cggccacctc
     1621 cttcgtgtgc cggggcattc tcgcacgggc aggcccggtt tccggcccga cggtcccgac
     1681 ataccatggg ccgatgccca ccaccgccgc cctgtcggac gccgtccgca ccttcctcga
     1741 ccagcccaac gtcgcctccg tcgccacgac cgacgacgac gggcagcccc gccaggcggt
     1801 cgtctggtac cgcctcgagc ccgatggccg gatcctcatc aactccagga cgccgcgccg
     1861 ctggtgcgcg aacctggtcc gcgatccgag gctcgccatc tcgatcatcg acgccgagga
     1921 cgcctaccgc tggatcgggt tgaccggggt cgtcgaggag gtcgtcgacg acgtctcgcg
     1981 atcgagggag gacatcgtcg ccctcgccta tcgctaccgt cccgaggggc ccaccgaggc
     2041 gtccatcgcg atgttccggt cgcagccgag agtgacgttc ctcgcccgga tcaccggcat
     2101 ccacgaccac ctcgaggact gatccgtggc ctcggtgaag ctcggcgtcc tgctctggcc
     2161 gcagaccagg agctggccgg acatccgcga cgccgccgcc ctcgccgacc gctcgggcgc
     2221 cgcgagcctg tggacgtggg accacctcaa cagcatcgtc ggtccgtggg aggggccgat
     2281 cttcgagggc tggtcgatcc tggccggctg gtcgcaggtc accgagcggg cgacgctggg
     2341 gctgatggtt gccgccaaca cgttccgcaa cccggggctg accgccaagc tcgcgacgac
     2401 gctcgaccac ctctccggtg ggcgggcgat cctcggtctc ggcggggcct ggttcgagcg
     2461 ggagcacgag gcctacggga tcgacttcgg cagcgggttc ggggagcggc tcgaccggct
     2521 ggacgaggcg gtgatgctcg tccggcggct gctcgacggc gagaccgtga cccacgacgg
     2581 acccgtctac ccgatgcgcg acgcgctcgt caccccgcgc ccggtgcagg tgcggctgcc
     2641 gatcaggatc ggcgggtcgg ggcctaagca gacgctccgg acgctggccc ggtacggcga
     2701 ccagtggaac gccatgggcg agctgccgaa gcgggccgag cgcgacgcga tcctccgcga
     2761 gcactgcgcg accttcgggc gcgacgacgc ggagatcgag cgcacgacca ccgtcgacat
     2821 cgtgat
//
LOCUS       DBNHXO010000178         5318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3614985, whole genome shotgun sequence.
ACCESSION   DBNHXO010000178 DBNHXO010000000
VERSION     DBNHXO010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3614985"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1013
                     /locus_tag="AC162A_03780"
     CDS             192..1013
                     /locus_tag="AC162A_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013540.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5009989.1"
                     /translation="MSPAARTALQRLAAEGGQAIRDERLRRRWTMRELAQRAGVALAT
                     LHSVESGEVASLESYARLGAALGLRPTLEFADSRARRSSPGGEDFVHAAMGEYEARAL
                     ARPGVRISIDEPYQHYQFAGRADVLAWDDENLLHIENRTRFPNLQEAAGSYNAKRQYL
                     APSMARRLDLGSHGWRSVTHVMACLWSSEVIHVLRLRRASFTALCPDPVDALAAWMAG
                     DLPARPGVTSMLVLIDPAAQGGTRTRSMVGLAEIARVRPRYRGYADAADALRRAR"
     gene            complement(1020..1973)
                     /locus_tag="AC162A_03785"
     CDS             complement(1020..1973)
                     /locus_tag="AC162A_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009990.1"
                     /translation="MTRFDLRLAFRLNRFEIVVLGLLIGVVGVAAVLVAGNLDATGYG
                     TTCLALGPESPMPPACEEAGRAFYDIQNKQASPVQTLLIVLPFLLGVLVGVPLIAREL
                     ERGTSRLAWSLAPSRTRWFLTRLVPVLLAVFVVSLVAGLAADRLMGATEPTADPFRSF
                     AGFGGRGIVLAARATFIFAIGVAVGSVIGSVLPALIVTAAIAVIGLSGGSSVHNRILA
                     SEAVMAVGVNGDARPGDLFIDQRIRTPGGDIVTWEELYVLLPAPDDGTEWPPPDYTFV
                     ALVVPAERYPEVQLREVGALAAGSLVALLIAGIVVRVRRPG"
     gene            complement(1970..2842)
                     /locus_tag="AC162A_03790"
     CDS             complement(1970..2842)
                     /locus_tag="AC162A_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5009991.1"
                     /translation="MAAALEAVGVGKRYRRGRPWALRSLDLAIPQGAITALVGPNGSG
                     KSTLIKGYVGFERPTTGRLLVGGVDPWKDRGGAIRQIGYVPQAASLYRELSVADHLSL
                     AARFRADFDRDAAVRRLDELDIPLHAPADELSGGQQAQVGLALALGTRAPVLLLDEPL
                     ASLDPLARREFLHVMVDAVRSVGATALLSSHVITDIEQACDRLLVLGGGRVLLDVSIA
                     EARAGHRVVEAGSDNADGIAQGLVGRFPDPDGRSLALVARSDETGRPATLEEIVLGHL
                     AAGRTRRGTAEVAA"
     gene            2973..4697
                     /locus_tag="AC162A_03795"
     CDS             2973..4697
                     /locus_tag="AC162A_03795"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5009992.1"
                     /translation="MGISRLWIDRVAIALGLIAVSIGVLTPIVAKSDTGVSLMVANGQ
                     VLVASVDPAGPGAAYGFEPGMVAVTLNDRFLVRLPEYVQPESDATPDPETGEVPAQDP
                     IVSPLVPTPEITGDAELRGLVDAPLVQLQAVRPADLAAWNVDTSGVSASIYDDGRASV
                     QDSILFVVFGIGVLFAIAWWLGTGRAGAEVVDLGLPLAVAAASPFLLQPLNELWAPLA
                     VAAHAVLIPLAMLPLAASLLNRIDGAEDQRIALVASATCAIGAAVIGSARSVSPSVSG
                     LSLAAGLLAAGIAAVPGLIAADRPLADPSRAGETPGNEATASGRVLRSAELAIAGATP
                     AVALLTVASSSPWLFVLWFAAIAVTGKFTVRPLVRLASRAQLQRDLVVAATEAERARV
                     AADIHDDALQELTLLVRRLEVAGDAEGAELARGVSDRLRAICGDLRLPILDDLGVGPA
                     LDWLVLRIEKLAGGTVRLERVDGARAPADVELAFFRIAQEALSNAVKHGSTPIVVRYH
                     ATDGGASLSIDDAGPGIAADAGERAEREGHFGLLNMQQRAEGIGAILDVRRWPAGGTH
                     VQLDWRQR"
     gene            4694..5314
                     /locus_tag="AC162A_03800"
     CDS             4694..5314
                     /locus_tag="AC162A_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5009993.1"
                     /translation="MIEGKAIRVAIVDDHPVVREGTAALLAAQPGLEVVGTAGSLADA
                     HALIAGGGIDVLLLDIRLGTDSGLRLLADGDVRAPDAPAVVMLTAYDYPQYAEAALRH
                     GAAGFVLKTAPLAELRDAIERAAGGGLAYAVRPGRASAPRCSPRELDVVRLVVNGRSN
                     DEIAAELGIGAKTVETHLARLFERFGLASRTELAPRALREGWLELP"
BASE COUNT          741 a         1915 c         1947 g          715 t
ORIGIN      
        1 cggcgagcgc gcggacacgc tcagcgagcg tggaagccgc accgcggggg ctgattcgtg
       61 ggtcgggacg ctccatcggc gcgtttggac ccgccgtggg gcctcacctg ttcggatgat
      121 gcgaaaagtc ggcggtgagc gccacgatgt tcactccttc cgaaatgtcg ccttcgcgaa
      181 agtcggatcc gatgtccccg gcggctcgca ccgcgctgca gcggctcgcc gccgagggtg
      241 gccaagccat ccgcgacgag cggctccggc gtcgctggac gatgcgtgag ctggcccagc
      301 gcgcaggcgt cgcgctcgcg accttgcata gcgtggaatc gggcgaggtc gcgagcctcg
      361 agtcgtatgc gcggctgggc gccgccctgg gcctgcggcc gacgctcgag ttcgcggact
      421 cccgtgcccg acgctcgtcg cccggcggcg aggacttcgt gcacgcggcg atgggcgagt
      481 acgaggcacg cgcacttgcg cgaccgggcg tacgcatctc gatcgatgag ccgtaccagc
      541 actaccagtt cgcgggccgc gccgacgttc tggcgtggga cgacgagaac ctgctccaca
      601 tcgagaaccg gacgcgcttc cccaacctgc aggaggctgc gggctcgtac aacgccaagc
      661 ggcagtacct cgcgccatcg atggcacgac gactcgacct cggatcgcac ggctggcgga
      721 gcgtcaccca tgtgatggct tgcctgtggt cgagcgaggt gatccatgtg ctgcgccttc
      781 gtcgcgcgtc cttcacggcg ctgtgcccgg acccggtcga cgccttggcc gcgtggatgg
      841 ccggcgatct ccctgctcga cccggcgtga cgagcatgct cgtgctgatc gatccggcgg
      901 cgcagggtgg cacgcggaca aggtcgatgg tcggtctcgc ggagatcgcg cgggtcaggc
      961 cgaggtatcg cggctatgcc gacgccgccg acgccctgcg acgcgcccga tgatgcccct
     1021 cagccgggac ggcgcacgcg gacgacgatg ccggcgatga gcagcgcgac cagggagccg
     1081 gccgccagcg cgccgacctc ccgaagctgg acctccgggt accgctcggc gggcacgacc
     1141 agggccacga acgtgtagtc gggcggaggc cattcggtgc cgtcgtcggg cgctggaagc
     1201 agaacataga gctcctccca ggtcacgatg tcgccgcccg gggtccggat ccgctggtcg
     1261 atgaacaggt cgccgggtcg cgcatcgccg ttgacgccga cggccatcac ggcctccgag
     1321 gcgaggatcc ggttgtggac ggagctgccg cccgacagtc cgatgacggc gatcgcggcc
     1381 gtgacgatca gcgccggcag gacgctcccg atgacagagc cgacggcaac gccgatggcg
     1441 aagatgaacg tcgcccgggc cgcgagcacg atcccgcgcc cgccgaaccc ggcgaagctg
     1501 cgaaacggat ccgccgtcgg ctcggtggcg cccatcagcc ggtcggcggc gaggccggcg
     1561 accagcgaca ccacgaacac ggcgagcagc acgggcacca ggcgcgtcag gaaccagcgg
     1621 gttcgcgacg gcgccagcga ccaggcgagc cggctcgtcc cgcgttccag ctcgcgcgca
     1681 atgagcggga cgccgacgag gacgccgagc aggaacggca gcacgatgag cagggtctgg
     1741 accgggctcg cctgcttgtt ctggatgtcg tagaaggcac ggccggcctc ctcgcatgcc
     1801 ggcggcatcg gcgactccgg gcctagcgcg aggcaagtcg tgccgtaccc ggtcgcatcg
     1861 aggttgccgg cgacgagcac ggcggcgact ccgacgacgc cgatcaggag gccgagcacg
     1921 acgatctcga agcggttcag ccggaaggcg agccggaggt cgaagcgcgt cacgccgcca
     1981 cctcggccgt tccgcgccga gtgcggccgg ccgcgaggtg cccgagaacg atctcctcga
     2041 gcgtcgccgg gcggccggtc tcgtccgatc tcgcgaccag agccagcgac cggccgtcgg
     2101 gatcgggaaa tcgacccacg aggccttgcg cgatcccatc ggcgttgtcg gaccccgctt
     2161 cgacgactcg gtggcccgcc cgcgcctcgg caatcgacac gtccagcagc acccgcccac
     2221 cgccgagcac gagcagccgg tcgcacgcct gctcgatgtc ggtgatcacg tgggacgaga
     2281 gcagggcggt cgcgcccacc gagcgcacgg cgtcgaccat cacgtggagg aactcgcgcc
     2341 gcgcgagcgg gtcgaggctc gccagcggct cgtccaacag caggacgggg gcacgggtcc
     2401 cgagggcgag cgcgagcccg acctgggcct gctggcctcc cgagagctca tcggccgggg
     2461 catgcagcgg gatgtccagc tcatcgaggc gccgaacggc agcgtcgcga tcgaagtcgg
     2521 cacggaagcg ggcggcgagg gacaggtggt cggccaccga cagctcgcgg tagagcgacg
     2581 ccgcctgcgg gacgtagccg atctggcgga tcgcgccgcc gcggtccttc catggatcga
     2641 cgcccccgac caggagccgg ccggtcgtcg gacgctcgaa cccgacgtag cccttgatca
     2701 gcgtcgactt gccggagccg ttcggcccga ccaacgccgt gatggcgccc tgcgggatcg
     2761 cgaggtcaag ggaccgcagc gcccaggggc gaccacgccg atatcgcttg ccgacgccga
     2821 cggcctcgag tgcagctgcc acgcggaacc ctccctccag gttgtcgcca gcctggcgcg
     2881 aagagtggcc tgggcgcagc cgttgcccga cggacggggg ttccgggatt tcccggacgc
     2941 ggtcggacta ggccctctgc taccgtcgat ccatgggcat ttcccgcctg tggatcgacc
     3001 gcgtggccat cgccctcggg ctcatcgcgg tctcgatcgg ggtcctgacg ccgatcgtcg
     3061 cgaagtcgga caccggggtc tcgctcatgg tcgcgaacgg gcaggtcctc gttgcctcgg
     3121 tcgatccggc cgggcctggg gccgcgtacg gcttcgagcc cggcatggtc gccgtgacgc
     3181 tgaacgatcg cttcctggtg cgcctcccgg agtacgtcca gccggagagc gacgcgacgc
     3241 ccgacccgga gacgggcgag gtgcccgccc aggacccgat cgtcagcccc ctggtcccga
     3301 ccccggagat caccggagat gctgagctgc gcgggctcgt cgacgcgccg ctggtgcagc
     3361 tgcaggccgt ccggccggcc gacctcgcgg cctggaacgt ggacacgtcg ggcgtgtcgg
     3421 cctcgatcta tgacgacggc cgcgccagcg tccaggactc gatcctgttc gtggtgttcg
     3481 ggatcggcgt gctgttcgcg atcgcctggt ggctcgggac cgggcgggcg ggcgccgaag
     3541 tcgtggacct cggcctcccg ctcgccgtcg ccgcagcgtc gccgttcctg ctccagccgc
     3601 tcaacgagct gtgggcgccg ctggcggtcg ccgcgcacgc cgtgttgatc ccgctcgcga
     3661 tgctgccgct cgcagcgtcg ctgctgaacc ggatcgatgg ggccgaagac caacggatcg
     3721 cgctggtcgc ctcggcaacg tgcgcgatcg gggcggcggt catcggctcc gctcgctcgg
     3781 tcagcccgtc ggtgtccggc ctgagcctcg ccgcgggtct gctcgccgcc ggcatcgcgg
     3841 ccgtgcccgg gttgatcgcg gcggaccgac cgttggccga cccgtcgcgg gccggtgaga
     3901 cccccggcaa cgaggcgacg gccagcgggc gcgtcctgcg gtcggccgag ctcgcgatcg
     3961 cgggcgccac gcctgccgtc gcgctgctga ccgtggcctc ctcatcaccc tggctgttcg
     4021 tgctctggtt cgccgcgatc gcggtgaccg gcaagttcac cgtccgaccg ctggtccggc
     4081 tcgcgagccg cgcgcagctg cagcgcgatc tggtcgtagc cgccacggag gccgagcggg
     4141 cacgcgtcgc cgccgacatc cacgacgacg ccctccagga gctgaccctg ctcgtccggc
     4201 ggctcgaggt cgcaggcgac gccgaggggg ccgagctcgc ccggggcgtg tccgaccggc
     4261 tgcgagcgat ctgcggcgac ctgcgcctcc cgatcctcga cgacctcggc gtcgggccgg
     4321 cactggactg gctcgtcctc cggatcgaga agctcgccgg aggcaccgtc cgcctcgagc
     4381 gtgtcgacgg cgcgcgggcc ccggccgacg tcgagctggc gttcttccgg atcgcccagg
     4441 aggccctgtc gaacgccgtc aagcacggca gcacgcccat cgtcgtgcgc taccacgcca
     4501 cggacggcgg ggcgtcgctc tcgatcgacg acgcggggcc cgggatcgcc gccgacgccg
     4561 gggagcgcgc cgaacgcgag ggtcacttcg ggctgctcaa catgcagcag cgggcggagg
     4621 ggatcggcgc gatcctcgac gtccggcgat ggccggcggg cggcacgcac gtccagctcg
     4681 actggcgcca gcggtgatcg agggcaaggc gattcgtgtc gcgatcgtcg acgaccaccc
     4741 cgtcgttcgc gaggggaccg cggccctcct cgccgcccag ccgggcctcg aggtcgtcgg
     4801 aacggcgggc tccctggcgg acgcccacgc cctgatcgcc ggcggcggga tcgacgtcct
     4861 gctgctcgac atcaggctcg ggacggacag cgggttgcgg ctgctggcag acggcgacgt
     4921 ccgcgcaccc gatgccccgg ccgtcgtgat gctgaccgcg tacgactacc cgcagtacgc
     4981 ggaggccgcc ctgcgccacg gggcagccgg gttcgtgctc aagacggcgc cgctggccga
     5041 gctgcgcgat gcgatcgagc gggcggccgg cggcgggctc gcctacgccg tgcgcccggg
     5101 tcgcgccagc gcgccgaggt gctcgccgcg cgagctcgac gtcgtgcgcc tcgtcgtcaa
     5161 cggtcggagc aacgacgaga tcgccgcaga gctgggaatc ggggccaaga ccgtcgagac
     5221 ccacctggcg cgcctgttcg agcgcttcgg cctcgcgtcg cgcaccgagc tcgcaccacg
     5281 tgcgctgcgc gagggatggc tcgagctccc ctgaggtt
//
LOCUS       DBNHXO010000179         3750 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4011729, whole genome shotgun sequence.
ACCESSION   DBNHXO010000179 DBNHXO010000000
VERSION     DBNHXO010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4011729"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="AC162A_03805"
     CDS             complement(<1..572)
                     /locus_tag="AC162A_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5009994.1"
                     /translation="MDAAWPLPPATPAAPAVGARSGANPALLGLLIGAAVPALVASAA
                     WWLNQPYVAAVAGVGIVIGAAIGVVAGPRMAGPDWIGSTILAALVAPLIAGVVIAVLF
                     VIGMVASAEPALGLFYGLIYALVILVVAEIAGAPVTLPIGFIVAVLVRRAARMRPEWA
                     AFHVGLLVVAALVAGGLTLVAMAGTFGVVGA"
     gene            724..1551
                     /locus_tag="AC162A_03810"
     CDS             724..1551
                     /locus_tag="AC162A_03810"
                     /inference="COORDINATES: protein motif:HMM:NF017816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HIR5009995.1"
                     /translation="MGRVQEQAAWNGFDVAPSVTPSADRGGTTAAHGFGIAFNPATIG
                     MLLDGSDRATREQLITTLQRIHGNAAVQRLLASSGPTVQRWAVGLARNTSDCAVVVNW
                     MNANSPHRADSGWAKTRAQFSWGGDPAYTTADGVTTATVTSPTVTPTVTVDMPSWAPT
                     DPAMRTAWNAMIADLRAHEALHEGIARTWEGTLRSNLTDLSVTVPNRTITAFRSAVQA
                     EWNGWLRQHQADQTAIDPYSALLDCSGGGDTSASTGGAGGGDTAELTGLDEELDLSG"
     gene            complement(1568..2170)
                     /locus_tag="AC162A_03815"
     CDS             complement(1568..2170)
                     /locus_tag="AC162A_03815"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HIR5009996.1"
                     /translation="MRTRFIVAIAALMAASALVAVPEPAGSSAPSSARQIDSATLHAV
                     SISSPAGSTAMAIPRLDSATRSAGWLGPGSTFLEPGKRKSPTRRVTVDQPGTRAGVFN
                     KNYWRYDSNISWYGSEFFGSGTACGQTYTKTIMGVAHRTLPCGTMVTFRNPANGRQVT
                     VPVIDRGPYVAGRQWDMSRGLCDYLDHCYTGSIEWRWGGR"
     gene            complement(2214..2804)
                     /locus_tag="AC162A_03820"
     CDS             complement(2214..2804)
                     /locus_tag="AC162A_03820"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HIR5009997.1"
                     /translation="MRTRLFVAAPALIAVAALVTFADPAAMAAPGTARAFELADSRPL
                     ILDRPLPTVTTSLTTESDLASAGRLSAATPLLEPGGMAVPAARAATRQPAARPIRVVR
                     PRWRTAEYSWYGSAFFGSGTACGQRYSRTILGVASKTLPCGTRVTLRNPNNGRTITVP
                     VIDRGPYVSGRLWDLSRATCDYLRNCHTGTIKWRLP"
     gene            2875..>3750
                     /locus_tag="AC162A_03825"
     CDS             2875..>3750
                     /locus_tag="AC162A_03825"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR5009998.1"
                     /translation="MTNAGAPIAPDAERSIEVDGPLDLRRTLGIHRRGPGDPTLRFEA
                     SGSAWRSSRTPDGAVTLFIEARDGVVRGRAWGPGAEWALTRLGGLVGLDDDPAALVPQ
                     HAAIERAVRRLRGLRIGRTGVVLEALVPAILEQKVIGEEARRAWTGLVRRYGEDAPGP
                     PGMRLPPTPRTLAELPYYRYHPFGVEQRRADLIRRVAREAPRLEAMVEPASAPGGDPS
                     GAYAALRAFPGIGPWTAAEVGIRAFGDPDAVSVGDFHLPSMVTWALAGEPRGTDERML
                     ELLEAYRGQRGRVLRL"
BASE COUNT          561 a         1294 c         1407 g          488 t
ORIGIN      
        1 gcgccgacaa ccccgaacgt gcccgccatg gccaccagcg tcaagccgcc cgcgaccaac
       61 gccgccacga cgaggagccc gacgtggaac gccgcccatt caggccgcat ccgggcggcc
      121 cgcctgacca ggaccgcgac gatgaagccg atcggcaggg tgaccggcgc gccggcgatc
      181 tcggcgacga cgaggatgac cagggcatag atgaggccat agaacaagcc aagggcaggt
      241 tccgcgctcg ccaccatgcc gatgacgaac aggaccgcga tcacgacgcc ggcgatgagc
      301 ggagcgacga gcgcggcgag gatcgtcgac ccgatccagt ccggaccggc catgcgcggg
      361 ccggccacta ccccgatcgc cgcgccaatg acgatcccga cgccggcgac ggcggcgacg
      421 tacggctggt tcagccacca ggcggcgctc gcgacgagcg ccggcacggc ggcgccgatg
      481 agcaggccga gcagcgcagg gttcgcgccg gaccgggcgc cgacggcggg cgcggctggc
      541 gttgcgggcg ggagcggcca ggcggcgtcc atgacgatca cctctaaacg tgctacccat
      601 cggtagcacg gcaagtgtcg accaccaacc tgctaccgtc aagtagcact aaccgtgcag
      661 atctgcccga tcgggcaatc ggtcggcttc cgaggcccgg cgacgctcgg ctacggttcc
      721 cccgtgggca gggttcagga acaggccgcc tggaacggct tcgacgtcgc gccgtccgtc
      781 accccgtcgg cggaccgcgg tggcacgacc gccgcccacg gcttcggcat cgccttcaac
      841 ccggcgacga tcgggatgct cctcgacggc tcggaccgtg cgacccgcga gcagctgatc
      901 accacgctcc agcgcatcca cggcaatgcc gccgtgcagc gtctgcttgc ctcgtccggt
      961 cccacggtcc agcgatgggc cgtcgggctc gcccgcaaca catccgactg cgcggtggtc
     1021 gtgaactgga tgaacgccaa cagcccgcac cgcgcggaca gcggctgggc gaagacccgg
     1081 gcccagttct catggggcgg cgatcccgcg tacaccaccg cggacggcgt cacgaccgcg
     1141 accgtcacca gcccgacggt cacgccgacc gtgaccgtcg acatgccctc gtgggcgccg
     1201 accgacccgg cgatgcgcac ggcctggaac gcgatgatcg ccgacctgcg cgcccacgag
     1261 gccctccacg agggaatcgc gaggacgtgg gagggcaccc tgcggtccaa cctgaccgac
     1321 ctgtcggtca cggtccccaa ccggaccatc acggccttcc gctcggccgt ccaggcggag
     1381 tggaacgggt ggcttcgtca gcaccaggcc gatcagacgg cgatcgaccc gtacagcgcg
     1441 ctgcttgact gttcgggcgg gggcgacaca tcggcgagca cgggtggggc cggtggcggc
     1501 gacacggcgg agctcaccgg cctggacgag gagctcgacc ttagcgggta gggaggctaa
     1561 cgaccgctca gcggcctccc cagcgccatt cgatcgatcc cgtgtagcag tggtcgaggt
     1621 agtcgcacag gccgcgcgac atgtcccact ggcggccggc gacgtagggg ccgcggtcga
     1681 tcaccggcac cgtgacctgg cggccgttgg ccgggtttcg gaatgtgacc atcgtcccgc
     1741 acggcagcgt ccggtgggcc acgcccatga tggtcttcgt gtacgtctgg ccgcacgcgg
     1801 tcccggagcc gaagaactcc gacccgtacc agctgatgtt gctgtcgtag cgccagtagt
     1861 tcttgttgaa cacgccggcc cgggtgcccg gctgatcgac cgtcacgcga cgggtgggcg
     1921 acttgcgctt gccgggctca aggaacgtcg aaccgggccc gagccatcca gccgagcggg
     1981 tcgccgagtc gagccgtgga atcgccatcg ccgtggaacc ggcgggcgac gaaatcgaaa
     2041 cggcgtgcag ggtcgccgaa tcgatctgcc tggcggagct cggggcactc gagcccgcgg
     2101 gttcgggtac ggcgaccagg gcggacgcgg ccatgagtgc cgcgatcgcg acgatgaacc
     2161 tggtgcgcac gataggaggt caggctaccc gggggtgtca agcccatcgg ccgtcacggc
     2221 agccgccact tgatcgtgcc cgtgtggcag ttgcgcaggt agtcgcaggt ggcgcgggac
     2281 aggtcccaca ggcgcccgct cacatacggc cctcgatcga tgacgggcac ggtgatggtt
     2341 cggccgttgt tcgggttgcg cagggtgaca cgggttccgc aggggagcgt cttgctggcc
     2401 acgccgagga tcgtccgcga atagcgctgg ccgcaggccg tgcccgaacc gaagaacgcc
     2461 gagccgtacc aggagtattc ggcggtgcgc caccgcgggc gcacgaccct gatcggccgg
     2521 gcggcgggct ggcgggtcgc cgcccgcgcg gcaggcacgg ccatcccacc cggctcgagg
     2581 agcggggttg ctgcgctgag tcgtcctgcc gacgcgaggt cggattcggt ggtgaggctc
     2641 gtcgtcacgg ttggtagggg tcggtcgagg atgagcggcc gactgtcggc cagttcgaac
     2701 gcccgtgcag tgccgggtgc cgccatggcg gccggatcgg cgaacgtgac cagggcggcg
     2761 acggcgatga gcgccggagc cgcgacgaac agcctggttc gcacggtgtg cccaacggta
     2821 ggctggcggg catgtcggga tgggttcgga tcggttccgc gggatttcgt tagcgtgacg
     2881 aatgccggcg cgccgatcgc gcccgacgcc gagcgctcga tcgaggtgga cggtccgctc
     2941 gacctccggc ggacgctcgg gatccatcgc cgcggcccgg gcgacccgac gctgcggttc
     3001 gaggcatcgg ggtccgcatg gcgctcgtcg cggaccccgg acggcgcggt gacgctgttc
     3061 atcgaggcac gcgacggcgt cgtacgcggg cgagcctggg gcccgggtgc cgagtgggcg
     3121 ctgacgcgac tcggcggcct ggtcggcctc gacgacgatc cggccgcgct cgtgccccag
     3181 cacgcggcga tcgagcgcgc cgtccggcgc ctgcgcggcc tgcggatcgg gcgaacgggc
     3241 gtcgtgctcg aggcgctggt cccggcgatc ctcgagcaga aggtgatcgg cgaggaggcg
     3301 cggcgggcgt ggacggggct tgtccggcgc tacggcgagg acgcgcccgg gccaccgggg
     3361 atgcggctgc cgccgacgcc ccggacgctg gccgagttgc cctactaccg ctatcacccg
     3421 ttcggggtcg agcagcgccg ggcggacctg atccggcggg tggccaggga ggcgccacgg
     3481 ctggaggcga tggtggagcc ggcgtcggcg ccgggcggag atccctccgg ggcctacgcg
     3541 gccctcaggg cgtttccggg gatcgggccg tggacggcgg ccgaggtggg gatccgggcg
     3601 ttcggggacc ccgacgcggt gagcgtcggc gacttccacc tgccgtcgat ggtcacctgg
     3661 gccctcgccg gcgaaccgcg cgggaccgac gagcggatgc tggagctgct cgaggcctac
     3721 cgcggtcagc ggggtcgcgt gctgcgcctg
//
LOCUS       DBNHXO010000180         3717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2622633, whole genome shotgun sequence.
ACCESSION   DBNHXO010000180 DBNHXO010000000
VERSION     DBNHXO010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2622633"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1079)
                     /locus_tag="AC162A_03830"
     CDS             complement(21..1079)
                     /locus_tag="AC162A_03830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR5009999.1"
                     /translation="MNEPELRAWFDERVATSAFRGVAIAWRDGAPIFEYAGGLAHRGL
                     GVPVALDSRFAVASITKMATAVTALRLVDRGLLRLDQPLIEVLPPEQQPDALTAEHTL
                     HHLLSHTSGLTNYHDDDDASGASWMSCWDRVATYHARRPADILPLFKDLPARAAPGAE
                     YRYGDANFILVGLVIEAVTGRAYGDVVAEEVFGPAGLQDTAIEELDDDPRRLAVGYKV
                     DDGPADRWKNNMYSVTASGMPDGGMITTAHDLARLIDAILGGRLLPAPLVAAMTSPQG
                     PNRTDLEQYGYGCELAVVDGSVAIIGHGGGDPGISAMLTRHLAAGITLSVVCNQDRGS
                     WAATKRLAEAFGLNEPRE"
     gene            1119..1805
                     /locus_tag="AC162A_03835"
     CDS             1119..1805
                     /locus_tag="AC162A_03835"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR5010000.1"
                     /translation="MSQSAARRIVDLSHPIEAGMVTYPGLPVPEIHAVLDRATSAGRY
                     APGVTFQIDVITLCGNTGTYMDSPFHRWEDGADLAALPPERLIDLPAVRIDVTGSATP
                     AIDASAFAGHGLAGRAVLVHTGFDRFWRTEAYLRDNPFLTRDAVELLVEAGAALVGID
                     SVNIDSTTDAERPAHSLLLRAGIPIVEHMTNLAAIPVDGARFTAIPAPVRGTGTFPVR
                     ALAVVPGDAT"
     gene            1879..3486
                     /locus_tag="AC162A_03840"
     CDS             1879..3486
                     /locus_tag="AC162A_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012613.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR5010001.1"
                     /translation="MPSKRRSALVVVLVAWLVALASPAAVAAGSGRFVDDNSSKYENA
                     IEAMVKAGVMSPCDKRHDRFCPNRSVSRGEMAGFLVKAFNLSATSGVHFKDVTKHTPH
                     ATAINRVVTAGIASGCSPKKFCPNSHVSRGRMAAYLAKALHLTATSTKRYKDVPKSNP
                     FATAISRLSKAGIAIGCGNGKFCPGRDITRAETAGFLERALTITRVSAQQPTPGNPTG
                     GAAIPPGAGEADASVPDHVVGSGTPASCTSKAVVAAVAKGGVITFDCGPSPVTIYMSA
                     TAKVFNNKPDVVIDGGGLVTLNGMGDRRILYMNTCDPDLVWTTSHCQNQAHPTLTVQN
                     IAFTRGRSSGHETEDGGGAIFVRGGRFRVVNAGFYGNRCASTGPDIGGASLQVFSQHD
                     GLPVYVVNSTFGGSGSRRNECSNAGGISSIGVSWTILNSVFTHNRAIGSGANPPKAGK
                     PGGGNGGAIYNDGNEMTLRVEGTRIEGNWSNHEGGSGIFFVSNNRTGFVVIKDSILRN
                     NDGDGFQTHPGIFFLGDEITFTNSIVE"
BASE COUNT          571 a         1350 c         1264 g          532 t
ORIGIN      
        1 gaacgtcgtc cgcccgatcg tcactcgcga ggctcgttga gcccgaacgc ctccgcgagg
       61 cgcttggtcg ccgcccacga gccgcgatcc tggttgcaca cgaccgagag cgtgatcccc
      121 gcggcgaggt ggcgcgtgag catcgccgag atcccgggat ctccgccgcc gtggccgatg
      181 atcgcgaccg acccgtccac caccgccagc tcgcacccgt agccgtactg ctcgagatcc
      241 gtccggttgg gcccttgcgg gctggtcatc gcggcgacga gcggggccgg cagcaggcga
      301 ccgccgagga tcgcgtcgat cagccgggcg aggtcgtggg ccgtcgtgat catcccgccg
      361 tcgggcatgc cgctcgcggt gacgctgtac atgttgttct tccaccggtc ggccggtccg
      421 tcgtcgacct tgtagccgac ggcgagccgc ctcgggtcgt cgtcgagctc ctcgatcgcc
      481 gtgtcctgga gacctgccgg cccgaacacc tcctcggcga cgacgtcgcc gtaggcgcga
      541 cccgtcacgg cctcgatcac caggcccacg aggatgaagt tggcgtcgcc atagcggtac
      601 tccgcccccg gtgcggcacg cgccgggagg tccttgaaca gcgggaggat gtccgccggc
      661 cggcgcgcgt gataggtcgc gacccggtcc cagcacgaca tccacgacgc cccgctcgcg
      721 tcgtcgtcgt cgtggtagtt ggtcagcccc gaggtgtgcg acaggaggtg gtggagcgtg
      781 tgctccgccg tcagcgcgtc cggctgctgc tcaggcggca ggacctcgat cagcggctgg
      841 tccagccgaa gcaggccgcg gtcgacgagg cggagggcgg tcaccgccgt ggccatcttc
      901 gtgatcgagg cgaccgcgaa ccgcgagtcg agcgcgacgg ggacgccgag cccgcgatgc
      961 gcgagcccgc cggcgtactc gaagatcggg gcgccgtctc gccaggcgat cgcgacgcca
     1021 cggaaggcgc tggtggcgac tcgctcgtcg aaccaggcgc ggagctcggg ctcgttcatc
     1081 gttcgattgt cgccggtcgc agccgatacg atgcggtcat gtctcagtca gctgcccgcc
     1141 ggatcgtcga tctcagccac ccgatcgagg ccggcatggt cacctaccct ggcctgcccg
     1201 tccccgagat ccacgcggtc ctcgaccgcg ccacgagcgc cggccgttac gcgcccgggg
     1261 tgacgttcca gatcgacgtc atcacgctgt gcgggaacac gggcacctac atggacagcc
     1321 cgttccaccg ctgggaggac ggggcagacc tggcagcgct gcctccggag cgcctgatcg
     1381 acctgccggc tgtccgcatc gacgtcaccg ggagtgcgac gccggccatc gatgcctcgg
     1441 cctttgccgg ccatgggctc gcgggtcgcg cggtcctcgt tcacaccggg ttcgaccggt
     1501 tctggcggac cgaggcctac ctgcgcgaca acccgttcct gacccgcgac gccgtcgagc
     1561 tgctggtgga ggccggcgcc gccctcgtcg gcatcgactc cgtcaacatc gactcgacca
     1621 cggacgccga gcgcccagcg cactcgctcc tgctccgggc gggcatcccg atcgtcgagc
     1681 acatgaccaa cctggccgcg atccctgtcg atggcgcgcg gttcacggcg atcccggcgc
     1741 ccgttcgcgg gaccggaacg ttccccgtcc gggcgctcgc ggtggtgcca ggagacgcca
     1801 cctagggcga ccaaggtccg gacaccgtcg gtaccgatgg cgcttgtcgc tccctgggcg
     1861 cgctggtagg ttcggccgat gccgagcaag cgccgttccg ccctcgttgt cgtgctcgtc
     1921 gcctggctgg tcgccctcgc gagcccggcc gcagtggccg ccggttccgg ccggttcgtc
     1981 gacgacaaca gctcgaagta cgagaacgcg atcgaggcca tggtcaaggc gggggtcatg
     2041 agcccctgcg acaagcgcca cgaccggttc tgccccaacc gcagcgtcag ccggggcgag
     2101 atggccggct tcctcgtgaa ggccttcaac ctctcggcca ccagcggcgt ccacttcaag
     2161 gacgtgacga agcacacccc ccacgcgacc gccatcaacc gcgtggtcac cgccgggatc
     2221 gcctcgggct gcagtccgaa gaagttctgc cccaacagcc acgtatcacg gggtcggatg
     2281 gccgcgtacc tggcgaaggc gctccacctg acggcgacga gcaccaagcg ctacaaggac
     2341 gtccccaaga gcaacccctt tgcgacggcg atcagcaggc tgtccaaggc ggggatcgcg
     2401 atcggctgcg gcaacggcaa gttctgcccc ggccgggaca tcacgcgcgc cgagacggcc
     2461 ggcttcctcg agcgcgccct gacgatcacg cgcgtcagcg cgcagcagcc gacgcccggc
     2521 aacccgaccg gtggcgcggc gatcccgccg ggcgcgggtg aggccgacgc gagcgtgccc
     2581 gaccacgtgg tcggcagcgg gacccccgcg agctgcacct cgaaggccgt cgtcgcggcg
     2641 gtcgccaagg gcggggtcat caccttcgac tgcggtccca gcccggtcac gatctacatg
     2701 agcgccacgg ccaaggtgtt caacaacaag ccggacgtgg tcatcgacgg cggtggcctg
     2761 gtcaccctca acggcatggg cgaccgccgg atcctgtaca tgaacacctg tgacccggac
     2821 ctcgtctgga cgacgagcca ctgccagaac caggcccacc cgacgctcac ggtgcagaac
     2881 atcgccttca cgcgcgggcg ctcgagcggg cacgagacgg aggatggcgg cggcgcgatc
     2941 ttcgtccgcg gcgggcggtt ccgcgtcgtc aacgccggct tctacggcaa tcgatgcgcg
     3001 tcgacaggcc ccgacatcgg tggcgcgtcg ctgcaggtgt tctcgcagca cgacggcctc
     3061 cccgtgtatg tcgtgaacag cacgttcggc ggttcgggct cgcggcgcaa cgagtgctcc
     3121 aacgccggcg ggatctcgag catcggcgtg tcctggacga tcctcaacag cgtgttcacc
     3181 cacaaccggg ccatcgggag cggggcgaac ccgcccaagg ccggcaagcc cggcggcggc
     3241 aacggcggcg cgatctacaa cgacggcaac gagatgacgc tgcgggtcga ggggacgcgg
     3301 atcgagggca actggtccaa ccacgagggc ggcagcggga tcttcttcgt cagcaacaac
     3361 cggacggggt tcgtggtgat caaggactcg atcctgcgca acaacgacgg cgacgggttc
     3421 cagacccacc ccggcatctt cttcctgggc gacgagatca cgttcacgaa ctcgatcgtc
     3481 gagtaggacc aggcccccag cgggccgatt cgccgtcgcg ctggccgccc ggaccggcca
     3541 caatgcctcc gcgagatccc ccggacccgg tgacgctccg acgcgacacc gcggcctgaa
     3601 cgggccgcac ggaggccgcc gatgaccgca cagatccgcc gccacccgct ggtggcgtac
     3661 tacgtgctcg cggtcgcgat ctccgtcgcg ctcgggctgc tgctcaacgt gtcgttg
//
LOCUS       DBNHXO010000181         4268 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1434522, whole genome shotgun sequence.
ACCESSION   DBNHXO010000181 DBNHXO010000000
VERSION     DBNHXO010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1434522"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="AC162A_03845"
     CDS             <1..497
                     /locus_tag="AC162A_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5010002.1"
                     /translation="AMLPDNVSHGGTYAGNRVAAAAAAKTLEILRDTDALATIHATGS
                     KIQDGIRGLLDAKGLAYHLTGHPSMFGIMFTERVATEYRDWANTDHELYDAIAIGMQA
                     RGAMPEPDSREPWFMCEAHAQGDTIDRIISIFGSSLDAALEARAHGELAADATGAMSS
                     HAAG"
     gene            553..1323
                     /locus_tag="AC162A_03850"
     CDS             553..1323
                     /locus_tag="AC162A_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR5010003.1"
                     /translation="MTLDGNAVRSVDRAAALLLALGDSHGEAGVTELARRLGLHKSTA
                     SRLLATLEKRGLVEQDEESGKYRLGLVVIRLAERAERTLDLRSIAMPELDRLARATRE
                     TTGLGVLDGDQLLTVAQADGPNLVAMGDWTGRCVPLHSVAGGKVLLASLPEREVLRLV
                     RRGIDRFTDRTITDLEPLLEELARVRRRGFATGFGEYDAGLNAVAAPVHDARGQVIAA
                     VDVWGPAFRVTPRRVPELVQQVRETAGAVSVRLGGTPA"
     gene            1505..>4268
                     /locus_tag="AC162A_03855"
     CDS             1505..>4268
                     /locus_tag="AC162A_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7507 domain-containing protein"
                     /protein_id="HIR5010004.1"
                     /translation="MPQSPASAHPHAVRVARILPVFAVATLLISLFAAVPPALADPAT
                     GPGAVSSTVAGALTAGTVPDGACRARITAVGGGGSSVPTTGASGGTGGAGASITATFA
                     VVPGQAYGGSVGGGGIGTTGGTGSGAIGTGGAGGTIVTDHRGGGGGGRTSVDLAGTTV
                     IVAGGGGGGGAAHNATPRGVGGGGGFTGIAAGGVAVGTTGQTGVDVPAATTVGGGQGG
                     QAAAGGTGGVNSATAARNGAAGGTIGSGTGGNGGPDPNYDSGGGGGGGYTGGGGGAST
                     NDQSVTGGGGGGGSSWVRGTAPVPAATVPTSVSGVAGTASPTGVGNGATGSIAIDWLP
                     CLYQLSVSKSVSSATVNAGGKVTWTVIVTNTGPDPMTRGDTVTLGDTLPAGPNGGPAP
                     AWRVTAFNVSGGSDTNLASGSLTCSGVSVGASMPASTTCSRPYASLSGAPSGGVRGLN
                     AGESITISYEQIIANTAACSTITNTASVVDRATQTGTTDITGVTATRTASASLTISCY
                     DLSITKVASPKPSVDQGGVITWTVTVTNSGPGSMNGPDSTDANPLVVSDTFPATGVGA
                     ATLSSATGPAGPCSLAGSTVTCTSGLPAGAQEVLVFTQTVGGAAAPGTVVSNTASGAD
                     PKTGDTNDSSTDQTTIQNLSLALVKTANPGTFAAAGATISYQYQVRNDGGARIAGPVT
                     VADNKATASCPALTTVGNSDAFLDPGETVTCSATYTITAGDVTAKSVTNTATASASGI
                     TSPVASQVVYLRHLSLTKSAAETSFNAVGNVLHYTLTATNDGGTTLFGVSIVDPVLGV
                     LSCAPAQPATLAPAATLTCSGTYSVTQADIDAGHRDNTATASSTDITGASVPATAATS
                     VNAVQTATLSLVKSATETSYNAVGDVLHYSYLVRNTGNVTLSGPITVTDDKTTVTCPP
                     TATLAPN"
BASE COUNT          638 a         1610 c         1503 g          517 t
ORIGIN      
        1 tggcgatgct gcccgacaac gtcagccacg gcggcaccta cgccggcaac cgcgtcgcgg
       61 ccgcggccgc cgcgaagacc ctcgagatcc tgcgcgacac cgacgcgctc gcgacgatcc
      121 acgccacggg atcgaagatc caggacggca tccggggcct gctcgacgcc aagggtctcg
      181 cctaccacct gaccgggcac ccatcgatgt tcggaatcat gttcaccgag cgggtcgcga
      241 ccgaataccg cgactgggcg aacacggacc acgagctgta cgacgcgatc gcgatcggga
      301 tgcaggcccg cggcgccatg cccgagccgg acagccggga accctggttc atgtgcgagg
      361 cgcacgccca gggcgacacc atcgatcgga tcatctccat cttcgggtcg tcgctcgacg
      421 ccgcgctcga ggcccgggcc cacggcgagc tcgccgcgga cgccacgggc gcgatgtcca
      481 gccacgcggc cggctgacgc cggcagtcga tcgaacgatc aaccaccgaa cccaccggga
      541 gggtcgcatc gcatgacgct cgacgggaac gccgtccggt cggttgaccg ggccgccgcc
      601 ctgctgctgg ccctggggga cagccacggc gaggcgggcg tgacggagct ggcgcgccgt
      661 ctgggcctgc acaagtccac ggcgtcgcgg ctgctcgcga cgctcgagaa gcgcggcctc
      721 gtggagcagg acgaggagag cggcaagtac cgcctcggcc tcgtcgtcat ccggctcgcc
      781 gagcgcgccg agcgcacgct cgacctgcgc tcgatcgcca tgcccgagct cgaccggctg
      841 gcccgcgcga cacgcgagac gacgggcctg ggcgtgctcg acggggatca gctgctcacg
      901 gtcgcccagg cggacgggcc caacctcgtc gcgatggggg actggaccgg gcgctgcgtg
      961 ccgctccact cggtcgccgg gggcaaggtg ctcctggcgt cgctgcccga gcgcgaggtt
     1021 ttgcgcctcg tgcgccgcgg catcgaccgg ttcacggacc gcacgatcac ggacctcgag
     1081 ccgctcctgg aagagctcgc gcgcgtccgg cgccgcgggt tcgccaccgg gttcggggag
     1141 tacgacgccg gcctcaacgc cgtggccgcg ccggtccacg acgcccgcgg ccaggtcatc
     1201 gcggcggtcg acgtgtgggg cccggcgttc cgggtgacgc cgcggcgcgt gcctgagctc
     1261 gtccagcagg tccgcgagac cgcgggcgcc gtctcggtgc ggctcggagg aacgcccgcc
     1321 tgacctccgg gcaacggccc gcctgacgct cgggccaccg cccgatcgtc gtcccgaccc
     1381 gtacgccagg ttcgaccggc gtcagtactt ccggcccagt tcgtgcgtgg accggtgcgc
     1441 ataggctcca cggagtgtcc ctggggggtc gcccgtccgt cacctagcgc cccggagtca
     1501 gctcttgcct cagtcccccg cgtcagcgca cccgcacgct gttcgcgttg cccggatcct
     1561 cccggtcttc gccgtcgcga cactgctcat ctcgctgttc gcggccgtcc cccccgccct
     1621 ggccgacccg gcaaccggtc cgggtgccgt cagcagcacc gttgcgggcg ccctgaccgc
     1681 ggggaccgtc ccggacggcg cgtgtcgcgc gcggatcact gccgtcggtg gcggaggatc
     1741 gtccgtgccg accacaggcg cgagcggtgg caccggtggc gcgggggcga gcatcacggc
     1801 gacgtttgcg gtcgtgcccg gccaggcgta cgggggcagc gtcggcggcg gcggcatcgg
     1861 gacgaccggg ggaacaggca gcggcgcgat cggcaccggc ggggccggtg ggaccatcgt
     1921 caccgaccat cgcggcggcg gaggcggcgg ccggacgtcg gtcgacctcg ccgggaccac
     1981 ggtcatcgtg gcgggcggtg gcggtggtgg cggggccgcg cacaatgcga cgccgcgcgg
     2041 cgtcggtggc ggtggcggct tcaccgggat cgcggccggt ggcgtcgccg tcgggacgac
     2101 cggtcagacc ggcgttgacg tgcccgcagc cacgacggtc ggcggaggcc agggtggcca
     2161 ggccgctgcc ggcgggacgg gcggcgtcaa ctcggcgacc gccgcccgca acggcgcggc
     2221 cggcggaacg atcggatccg ggaccggcgg caacggcggg cccgacccga actacgacag
     2281 cggtggtggt ggcggcggcg gctacaccgg cggcggcggc ggagcgtcga ccaacgacca
     2341 gtcggtaacc ggcggcggcg gtggcggcgg atcgagctgg gtgcgaggca cggcacctgt
     2401 cccggcggcg acggttccga cctccgtgtc cggcgtggcc gggacggcct cgccgacggg
     2461 cgtcggcaac ggcgcgaccg gatcgatcgc gatcgactgg ctgccctgcc tgtaccagct
     2521 ctcggtcagc aagtcggtct ccagcgcaac ggtgaacgcc ggcggcaagg tcacctggac
     2581 ggtcatcgtg accaacaccg gcccggaccc gatgacccgc ggcgacaccg tgacgctggg
     2641 tgacaccctg cccgccggcc ccaacggcgg gccggcaccg gcctggaggg tcaccgcctt
     2701 caacgtgtcg ggtggcagcg acaccaacct cgccagcggc agcctgacct gctcaggcgt
     2761 cagcgtcggc gcctcgatgc ctgcgagcac gacgtgctcc cggccgtacg ccagcctctc
     2821 cggcgccccg tcgggtgggg tccgcggcct caacgccggc gaatcgatca ccatcagcta
     2881 cgagcagatc atcgccaaca cggccgcctg ctcgacgatc acgaatacgg cgagcgtcgt
     2941 cgaccgcgcg acccagacag gcacgaccga catcacgggt gtcaccgcca cgcgaacggc
     3001 gagcgcctcg ctgacgatca gctgctacga cctctcgatc accaaggtgg ccagccccaa
     3061 accctcggtc gaccagggcg gcgtgatcac atggacggtg accgtcacca acagcggccc
     3121 cgggtcgatg aacggccccg actccacgga cgcgaacccg ctggtggtca gcgacacgtt
     3181 cccggccacg ggcgtggggg cggcgacact cagctccgcg acgggcccgg cgggaccgtg
     3241 cagcctcgcg ggcagcacgg tcacctgcac ctcgggcctg cctgcaggcg cgcaggaggt
     3301 gctggtcttc acgcagacgg tcgggggtgc cgcggcgccc gggacggtgg tctcgaacac
     3361 ggcatccggg gctgacccga agaccggcga cacgaacgac tcgagcaccg accagacgac
     3421 gatccagaac ctgtcgctcg cgctcgtgaa gaccgcgaat cctgggacgt tcgccgcggc
     3481 cggcgccacg atcagctacc agtaccaggt ccgcaacgac ggcggcgccc gcatcgcagg
     3541 gccggtcacc gtcgccgaca acaaggccac agcctcctgt ccggccctga ccaccgtcgg
     3601 caactccgac gccttcctgg atccgggtga gaccgtcacc tgctcggcga cctacacgat
     3661 cacggccggc gacgtcaccg cgaagtcggt gaccaacacg gccacggcgt cggcctcagg
     3721 gatcacgtcg cccgtggcaa gccaggtcgt ctacctccgt cacctgtcgc tgaccaagag
     3781 cgcagcggaa acgagcttca acgcggtcgg caacgtcctc cactacacgc tgacggcgac
     3841 caacgacggc gggaccaccc tcttcggcgt ctcgatcgtc gacccggtcc tgggcgtgct
     3901 ctcgtgcgcg cccgcgcagc cggccaccct ggcccccgcg gcgacgctca cctgctccgg
     3961 cacctactcg gtgacgcagg cggacatcga tgcgggccac cgcgacaaca cggcgacggc
     4021 ctcgtccacc gacatcaccg gtgccagcgt gccggcgacg gcggcgacgt ccgtcaacgc
     4081 cgtccagacc gcgaccctct cgctggtcaa gagcgccacc gagaccagct acaacgcggt
     4141 gggcgacgtc ctccactaca gctacctcgt gcgcaacacg ggcaacgtca ccctgtcggg
     4201 cccgatcacg gtgaccgacg acaagaccac cgtcacctgc ccgccgaccg cgaccctcgc
     4261 gcccaacg
//
LOCUS       DBNHXO010000182         1838 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3815747, whole genome shotgun sequence.
ACCESSION   DBNHXO010000182 DBNHXO010000000
VERSION     DBNHXO010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3815747"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1571
                     /gene="ilvD"
                     /locus_tag="AC162A_03860"
     CDS             <1..1571
                     /gene="ilvD"
                     /locus_tag="AC162A_03860"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR5010005.1"
                     /translation="MPCNLNQRRLAECVKEGIRAAGGTPMEFNTIAVSDGVSMGTEGM
                     KASLISREVIADSIELVARGHLFDGIVCLVGCDKTIPAGAMALGRLGIPGLVLYNGTI
                     YPGTYKGEPADIVTVYEAIGAYRAGKIGLEELYEIEEGACPGAGACGGQFTANTMATV
                     CEFLGLSPAGMNGIPAEDPAKEDAARRAGELVMTLVRHDILPGDIVTRNSFENAIASV
                     SATGGSTNAALHLPAIAAEFGLTLTLDEFAEVADRTPLIADMRPGGRYAAADMYNAGG
                     VGLVMRELLKRDLLHGDEKTVDGRTIAKVAADTVETPGQLVVRTIDNPIKETGGLTIL
                     RGSLAPDGCVLKLAGAERRHHSGPARVFDSEAACYEAVRERRIVEGDVVVIRYEGPVG
                     GPGMQEMLGVTGALVGAGLGQSVALLTDGRFSGGTRGLKIGHVSPEAAVGGPIALIEE
                     GDTITVDVDARELNLDVADDVLAARRARWSPPAPRYTTGALAKYAALVSSASEGAITS
                     GSQMRAALARSTGG"
     gene            1577..>1838
                     /locus_tag="AC162A_03865"
     CDS             1577..>1838
                     /locus_tag="AC162A_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010006.1"
                     /translation="MASTRPGLVGFRISASESTGLDWPRIDETWALAGELRVFDAGWM
                     SDHISDAMRERNGPAMESLTTVSALAHRVPGKWIGIAVAANTF"
BASE COUNT          273 a          649 c          678 g          238 t
ORIGIN      
        1 ccatgccctg caacctcaac cagcgccgcc ttgcggagtg cgtgaaggag ggcatccggg
       61 ccgcgggcgg caccccgatg gagttcaaca cgatcgcggt ctccgacggc gtctcgatgg
      121 gcaccgaagg catgaaggcc tcgctgatca gccgcgaggt catcgccgac tcgatcgagc
      181 tcgtcgcccg cggccacctg ttcgacggca tcgtgtgcct cgtcggctgc gacaagacca
      241 tcccggccgg ggcgatggcg ctcgggcgtc tcgggatccc gggcctcgtc ctctacaacg
      301 gcacgatcta ccccggcacg tacaagggcg agccggcgga catcgtcacg gtgtacgagg
      361 ccatcggcgc gtatcgggcg ggcaagatcg gcctcgagga gctgtacgag atcgaggagg
      421 gcgcctgccc gggcgcgggg gcgtgcggcg ggcagttcac cgccaacacg atggcgaccg
      481 tgtgtgaatt cctggggctc tcgccggcgg gcatgaacgg gatccccgcc gaggacccgg
      541 ccaaggagga cgccgcgcgc agggcgggcg agctcgtgat gacgctggtg cgccacgaca
      601 tcctgccggg cgacatcgtc acccgcaact cgttcgagaa cgcgatcgcg tccgtgtcgg
      661 ccaccggcgg cagcaccaac gcggccctcc acctgcccgc gatcgccgcg gagttcgggc
      721 tgaccctcac actcgacgag ttcgccgagg tcgccgaccg gacgccgctg atcgccgaca
      781 tgcgcccggg cggccggtac gccgcggcgg acatgtacaa cgccggcggc gtgggcctcg
      841 tgatgcgcga gctgctcaag cgcgacctcc tgcacggcga cgagaagacc gtcgacggcc
      901 gcacgatcgc gaaggtcgcc gccgacacgg tcgagacgcc cggccagctg gtcgtccgaa
      961 ccatcgacaa cccgatcaag gagaccggcg gcctgacgat cctccgcggc agcctcgccc
     1021 ccgacgggtg cgtgctcaag ctcgccggcg cggaacggcg tcaccacagc ggcccggcgc
     1081 gcgtgttcga ctcggaggcc gcgtgctacg aggcggtccg cgaacgcagg atcgtggaag
     1141 gcgacgtggt cgtgatccgg tacgagggcc cggtcggcgg ccccgggatg caggagatgc
     1201 tgggcgtcac gggcgcgctc gtcggcgcgg ggctcggtca atccgtggcc ctgctcacgg
     1261 acgggcggtt ctccggcggc acccgcggcc tgaagatcgg ccacgtgtcg cccgaggcgg
     1321 cggtcggcgg cccgatcgcg ctgatcgagg agggcgacac catcacggtc gacgtcgacg
     1381 cgagggagct caacctcgac gtcgcggacg acgtgctggc cgctcgccgg gcgcgctggt
     1441 cgccgcccgc gccgcggtac acgaccggcg cgctcgccaa gtacgcggcg ctggtgtcgt
     1501 cggcgtcgga gggggcgatc accagcggat cgcagatgcg ggcggcgctc gcccggagca
     1561 ccggcggcta gcgccgatgg ccagtacgcg gccgggcctc gtcggcttcc ggatctcggc
     1621 ctccgagtcg accgggctcg actggccgcg gatcgacgag acctgggcgc tcgccggaga
     1681 gctcagggtg ttcgacgccg gttggatgag cgatcacatc tccgacgcca tgcgcgagcg
     1741 caacggcccc gcgatggagt cgctgaccac ggtctcggcg ctcgcgcacc gcgtgcccgg
     1801 gaagtggatc ggcatcgcgg tcgcggccaa tacgtttc
//
LOCUS       DBNHXO010000183         3188 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2823890, whole genome shotgun sequence.
ACCESSION   DBNHXO010000183 DBNHXO010000000
VERSION     DBNHXO010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2823890"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="AC162A_03870"
     CDS             <1..335
                     /locus_tag="AC162A_03870"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoheptose isomerase family protein"
                     /protein_id="HIR5010007.1"
                     /translation="MSGEPDAEAGGRLMDEADLVLDVCSPAADATVTIAGLDTPVGPA
                     STVGNVAIVNAIKVRTAELLVERGAMPPVITRASVVGAERSRALFDEAYREHARRLAR
                     AIDQRGGG"
     gene            427..1497
                     /locus_tag="AC162A_03875"
     CDS             427..1497
                     /locus_tag="AC162A_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR5010008.1"
                     /translation="MKRNGIARVAVALAATVFVVGACSSGGGASAAPSGGAPAAYTVG
                     LSNPGAVGNGWREAMICSAKAQAVKQGNVTSVSVESEKTDAAGQLTQIRNLIAKGVKT
                     LLINPSSPDALNPAIKEAIDAGIAVVAIDAPVTAPGAYNLSNDQEEYAYLGAKWLFEH
                     LGGKGAVVYMRGIPGHQADTDRDTGFKKALAEYPGITVAKETSTEWDQKKGTDQINEI
                     LAAGTPFDGVWTSGIDNVIVDALVEADHVVPVVGADNAGFVKQLLEVEGLEGAAVTNP
                     AAVGGAGVVLGDQILKGTAPADHNVHVTPDLWENVTPEGKAKLTAAADPSLPAIWPLG
                     LTVKDWTTYDPAAVKACKGPGE"
     gene            1571..3148
                     /locus_tag="AC162A_03880"
     CDS             1571..3148
                     /locus_tag="AC162A_03880"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR5010009.1"
                     /translation="MTETTSDLLLDVSGVAKHYGAVAALRAASLAVRPGEVHALMGAN
                     GAGKSTLVKILTGAVRPDAGTIVVRGKPYAARSPREARQVGIVSVYQEPSLVPDLDIA
                     SNLRLTRTPVEPCRHWLAELGIPTLDLRATARNLPLATLRVIDLARALAIEPDVLMLD
                     EMTAALPADLTEKVLEVVGRRRGSNQSVIFISHRMIEIAAVCDRATVLREGETVGVVD
                     VEEGSEERIVKLMLGDIVREQAERTPAAAGAASTRAGTPRIRAKGLALGTKLTDVSFD
                     LYPGEVLGVVALEGQGQDELFDILAGSERPRSGQLSVDDVAVSFRHPADAIRAGLVFV
                     PADRAEALLMQRSVRENIALPFTAPLRGWGPIDARAEGKRVSQAVETLEIDTRAQSEV
                     RRLSGGNQQKVTIARWVAGGVRTLLCFDPTRGIDIGTKRQIYELMRELAGAGAAVLFY
                     TSELKEVQLVCDRTIVIFGGRVVAEIDVADADETALLRAAYDLPPDATMPEEVAASEL
                     AADSAGDAPAQPSEVVT"
BASE COUNT          503 a         1119 c         1132 g          434 t
ORIGIN      
        1 ccatgtcggg cgagcccgat gccgaggcag gcggacggct gatggacgag gccgacctgg
       61 tcctcgacgt ctgctcgccg gccgccgacg cgacggtgac gatcgcgggc ctcgacacgc
      121 cggtcgggcc ggcgtccacg gtcggcaacg tggccattgt caatgccatc aaggtccgga
      181 ccgccgagct cctcgtggag cgaggggcga tgcccccggt gatcacgcgc gcgagcgtcg
      241 tgggtgccga gcgatcgagg gccctgttcg atgaagccta tcgagagcat gcgcggcgtc
      301 tcgcccgtgc gatcgaccag cgaggaggtg gatgacggaa cgacgatcaa tcggcgagac
      361 tgcgcgaacg aggcggtcgg tgccccacag agggttgcgc gaccgcacga tggaggagtt
      421 gcatacatga agcgaaacgg aattgcccgc gtcgcggtcg cgcttgccgc gaccgtgttc
      481 gtcgtgggcg cttgctcgag cggaggcggc gcctctgcgg ccccctcggg gggtgctccg
      541 gccgcgtaca cggtcgggct gtcgaacccg ggcgccgtcg gcaacggctg gcgcgaggcg
      601 atgatctgct cggcgaaggc ccaggcggtc aagcagggca acgtcaccag cgtctcggtc
      661 gagtcggaga agaccgacgc ggccggccag ctcacccaga tccgcaacct gatcgcgaag
      721 ggcgtcaaga ccctgctgat caacccgtcc agcccggacg cgctcaaccc cgcgatcaag
      781 gaggcgatcg acgccgggat cgccgtcgtg gcgatcgacg cgccggtcac cgcccccggg
      841 gcgtacaacc tgtccaacga ccaggaggag tacgcgtacc tcggcgccaa gtggctgttc
      901 gagcacctcg gcggcaaggg cgccgtcgtc tacatgcgcg gcattccggg ccaccaggcc
      961 gacaccgacc gcgacacggg cttcaagaag gccctggccg agtaccccgg catcaccgtc
     1021 gccaaggaaa cctccaccga gtgggaccag aagaagggca ccgaccagat caacgagatc
     1081 ctcgcggccg gcacgccgtt cgacggcgtc tggacgtcgg gcatcgacaa cgtcatcgtt
     1141 gacgcgctcg tcgaggctga ccacgtcgtg ccggtcgtcg gcgccgacaa cgcaggcttc
     1201 gtcaagcagc ttctcgaggt cgaaggcctc gagggcgccg ctgtcaccaa tccggccgcc
     1261 gtcggcgggg cgggcgtggt gctcggcgat cagatcctga agggcacggc acctgccgat
     1321 cacaacgtcc acgtcacgcc cgacctgtgg gagaacgtga cccctgaagg caaggccaag
     1381 ctgaccgcag cggccgaccc gtccctgccg gcgatctggc ccctcggcct gaccgtcaag
     1441 gactggacga cctacgaccc ggccgccgtc aaggcgtgca agggccccgg cgagtaaccg
     1501 tctagccata tcgcgaccaa gcggggctgc cggtatcgcc ggcagccccg gcttcaaccc
     1561 taggggtccg atgaccgaga ccaccagcga cctcctgctc gatgtgagcg gggtcgcgaa
     1621 gcattacggg gcggttgccg cattgcgcgc cgcctcgctt gccgttcggc cgggcgaggt
     1681 gcacgcactg atgggcgcca acggcgcggg caagagcacg ctggtcaaga tcctgaccgg
     1741 tgccgtgcgg cccgacgccg gcacgatcgt cgtccgcggc aagccctatg ccgcgcgctc
     1801 gccgcgagag gcgaggcagg tcggcatcgt ctccgtgtac caggagccgt cgctcgttcc
     1861 tgacctggac atcgcgtcca acttgcgcct gacgcgcacc cccgtggagc cgtgccgcca
     1921 ttggctggcc gagctgggca tccccaccct ggacctgcgg gcaaccgcac ggaacctgcc
     1981 gctcgcaacg ctgcgagtca ttgacctcgc gcgcgccctg gcgatcgagc ccgacgtcct
     2041 catgctcgac gagatgaccg cggcgctgcc ggcggacctc accgagaagg tgctcgaggt
     2101 cgtcggccgg cggcgggggt caaaccagtc ggtgatcttc atctcgcacc ggatgatcga
     2161 gattgccgcc gtctgcgacc gggccaccgt gctgcgcgag ggcgagaccg tcggcgtcgt
     2221 cgacgtagag gagggctccg aggagcggat cgtcaagctg atgctcggcg acatcgtgcg
     2281 cgagcaggct gagcggacgc ccgcggcggc cggtgcggcc tcgacgcggg ccggcacgcc
     2341 gcgcatccgc gcgaagggcc tcgcgctcgg gaccaagctc accgacgtct cgttcgacct
     2401 gtacccgggc gaggtgctcg gggtcgtcgc actcgagggc cagggccagg acgagctgtt
     2461 cgacatcctc gccggctccg agcgaccgcg aagtgggcag ctgtcggtcg acgacgtggc
     2521 cgtctcgttc cggcatccgg cggacgccat ccgcgccggc ctcgtcttcg tgcccgcaga
     2581 ccgggcggag gccctgctga tgcagcgctc cgtgcgcgag aacatcgccc tcccgttcac
     2641 cgcgccgctc cgcggctggg gccccatcga cgcacgcgcc gagggcaagc gcgtctcgca
     2701 agcggtcgag accctggaga tcgacacccg cgcacagtcc gaggtccggc ggctgtcggg
     2761 tggcaaccag cagaaggtca cgatcgcgcg ctgggtggcc ggtggcgtgc ggaccctgct
     2821 gtgcttcgac ccgacccgcg gcatcgacat cgggaccaag cgccagatct acgagctgat
     2881 gcgcgagctc gccggcgcgg gcgccgcggt gctgttctac acctcggagc tcaaggaggt
     2941 gcaactcgtc tgcgatcgga ccatcgtcat cttcggcggt cgcgtcgtcg ccgagatcga
     3001 cgtcgcggac gcggacgaga cggccctgct gcgtgcggcg tatgacctgc cgccggatgc
     3061 gacgatgccc gaggaggtcg cggcgtcgga gctcgccgcg gacagcgcgg gtgatgcgcc
     3121 ggcccagccg agtgaggtgg tgacgtgagc gcggccgcgg gcatcgtccc cgagacccgc
     3181 cccgcccc
//
LOCUS       DBNHXO010000184         6579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4013957, whole genome shotgun sequence.
ACCESSION   DBNHXO010000184 DBNHXO010000000
VERSION     DBNHXO010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6579
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4013957"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="AC162A_03885"
     CDS             complement(<1..951)
                     /locus_tag="AC162A_03885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5010010.1"
                     /translation="MARPPLPAFIADVVDDRQARATLLAGAAALFAAGLDPKVWGPSL
                     TTVQSAIRARPELESYVLIGAVGFAIMLLIGGAIGDLRQARPLILGGLVVLVATDVIG
                     LVVTSGPAFIAVRLVGSAASALVVPASLACVALAYRGVARATAIGFAYAAYGGAQALM
                     PMLLTLIPGERWPGFVVAALAAALALAIARNRVPQLERPGRQERPYVLGTALWASSIV
                     AISAGLLWLGSGWDNPVRLAIVGLGVALLVAFFVAEARRRAEHPASLRVDRRPVTIAL
                     FAGVVIAIAQSVPMVLLPTYFAIVPRYGPLFGLAAVAPLFL"
     gene            complement(1011..1916)
                     /locus_tag="AC162A_03890"
     CDS             complement(1011..1916)
                     /locus_tag="AC162A_03890"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR5010011.1"
                     /translation="MSVLNELQEAVAAVAASVGPSVVGIGSRLRGSGVVVADGHVLTN
                     AHNLRGGEVTVRFPGGRTAVGTVKGVDWDGDLAVVEVDTAGAPPIAWSSGNVTIGSAV
                     FAAAATPSGGARVSFGFVSSVERAFRGPGGRRISGSFEHTAPLAPGSSGSPLLDADGA
                     LAGLNTNRVGEGFYLALPADAGLKSRVDALGRGEARERPHLGIAVAPAHVARRLRRSV
                     GLPELDGVLVRGVEAGSPAEAAGILEGDLLVAAGGKPIPDIDDLHDVLGETGLPLEVT
                     LVRGTEERTVTVGPAPASDGEPLVN"
     gene            2033..2482
                     /locus_tag="AC162A_03895"
     CDS             2033..2482
                     /locus_tag="AC162A_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR5010012.1"
                     /translation="MSSPVDASAHVPAAASALGVALDRVGDRWSLLIVEALLPGARRF
                     GELGESVTGIAPNILSDRLRRLERAGILVATPYSRRPVRMEYALTADGRELAGALHLL
                     ADWGVRRGGSGEPLRHDVCGTPLEARWHCPTCGRLVEDDEPDGLRRL"
     gene            complement(2514..4091)
                     /locus_tag="AC162A_03900"
     CDS             complement(2514..4091)
                     /locus_tag="AC162A_03900"
                     /inference="COORDINATES: protein motif:HMM:NF020610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha TIM-barrel
                     domain-containing protein"
                     /protein_id="HIR5010013.1"
                     /translation="MQQLAAGSDLEALLARANGLAVAWGSGAASSTTIGQERAILRLF
                     GVTGVDRAGRPLAAEVVDRYLGPDPRRLGGGIALPFAMALAEYDLAPQELGLAVAAGD
                     VDLGLEAELLAEPDRRAIALADASALSRAAMDRIDANRTARRELLAVLGDPPRPWLGT
                     GLGSAAIVDALDEARAAIDGGAELVRVDVPASRELAERTARTAAPVERWRAQPSSRGG
                     LDNFDPSGQPIPTGAQRSLAVLRRFVDEAGARRRGYIRIMTDAQALAAPDQAVVAAFE
                     RIDIVIADPMREIVAGRVDPDRAIADHVFAHRLLGRAGTRILVPAGPLIVARDLSSGV
                     PSDPATRAGRALALQLLAMALARRDGLPDDAVVLGAFPDWLVEESDAPARVAAEVALR
                     RALLPGHPLAFVEPPLRNDLATMWHAIVAALLPDAGDALAIVRRPGASAPETAQSRGA
                     TSVAASLRASRETPELRGMALDHANRAVAAANTTLRLLEEGGWGTLVDETLGFASGRL
                     GAEAVAERTEAFDPLGF"
     gene            complement(4153..4932)
                     /locus_tag="AC162A_03905"
     CDS             complement(4153..4932)
                     /locus_tag="AC162A_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HIR5010014.1"
                     /translation="MATDRLLPALRIRVRLFAMQREIAGMRELRLEVPVGSTVDDAWT
                     AVAGSVPALAAGRSSLRFAVNGTYADPDTTLADGDELACIPPVSGGSGDVAARILEIH
                     AEPFAADIVAALTARLATDEDGGVVAFTGRTRSTPGTPAPGQEDEAARHAGRKVDGLE
                     YETFESMAIAVLGEIADEVEARFGVRRLAIVHRSGAVPLGDASVVIVAVAPHRDAAFA
                     AARYAIDETKARAPIWKAERFTDGHVWIGHTARTSAEPPAR"
     gene            complement(4932..5780)
                     /locus_tag="AC162A_03910"
     CDS             complement(4932..5780)
                     /locus_tag="AC162A_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010015.1"
                     /translation="MPAEQIATNLALINWTLLTGLAVGSFAVVVLARLATPATKGYLA
                     FTAACAAGFGVLAYLSDTGLPAVGSGSPIPVNAAFDEPRRAALVAFVLLAVVTTVALA
                     RGRRAVLPAIAGLVAGLAVILLGALTWGGSPLGVVPLAIQLTVLAAATGGVFAAMILG
                     HWYLVTPRLPEAPLVMVSRRLLWIVALQVVLFVGWVGFGAGPGSAATGGGPFSALVGP
                     WALFVWLRLVIGLVFPLIVSWAAVQTARTRSMESATGLLYIDVGAIAAGTILAAGLYF
                     GAGLLV"
     gene            5891..>6579
                     /locus_tag="AC162A_03915"
     CDS             5891..>6579
                     /locus_tag="AC162A_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR5010016.1"
                     /translation="MAIGRDTTFTWLGHAGIEIATPGGQALLIDPWFGNPNSPRAVAD
                     QERADVLLVTHGHFDHFDADTLDLARRLHPTMPCVHELSLYLIAELGDAAGVIGMNAG
                     GTVEANGLKITMVPAAHSSGDLMGGDAVRYFGEAVGFVIELENGFRVYDSGDTTVFGD
                     MALIRELYRPDLAILPIGGHFTMDPAAAAVAVGLLGVRDVLPVHWGTFPILAGTPAAL
                     AAELAAREIVAT"
BASE COUNT          950 a         2478 c         2447 g          704 t
ORIGIN      
        1 gaggaacagg ggcgcgacgg cggccagccc gaacagcggt ccgtacctgg ggacgatcgc
       61 gaagtaggtc ggcagcagca ccatgggcac cgactgggcg atggcgatca cgaccccggc
      121 gaacagcgcg atggtcaccg gccggcggtc aacccgcagc gaggcgggat gctccgcgcg
      181 cctgcgggcc tccgccacga agaacgcgac gagcagcgcg acgcccagcc cgacgatcgc
      241 gagccgcacc gggttgtccc agccgctgcc gagccacagc aggccggccg agatcgccac
      301 gatgctgctc gcccagagcg ccgtcccgag gacgtacggc cgctcctggc ggcccgggcg
      361 ttcgagctgc gggacccggt tgcgcgcgat cgctagcgca agcgccgccg cgagggcggc
      421 caccacgaac cccggccatc gctcgccggg aatgagcgtg agcagcattg gcatcagggc
      481 ctgcgcgccg ccgtacgccg cgtaggcgaa gccgatcgcg gtcgctcggg cgaccccgcg
      541 ataggcgagc gcgacacacg cgagcgacgc cgggacgacg agagccgacg cggcggatcc
      601 gacgaggcgg accgcgatga aggccggccc ggaagtcacg accaacccga tgacgtccgt
      661 cgccacgagc acgacgagcc cgcccaggat cagcggccga gcctgccgca ggtccccgat
      721 ggccccgccg atcaggagca tgatcgcgaa gccgacggcc ccgataagca cgtacgactc
      781 gagctcgggc cgggcgcgga tcgcagactg caccgtcgtc agcgacgggc cccagacctt
      841 gggatcaagg ccggccgcga acagggcggc cgctcccgcc aggagcgtcg cccgcgcctg
      901 ccggtcgtcg acgacgtcgg cgatgaacgc aggcagcggc ggtcgagcca tacggcgcga
      961 tgctagcagc gggtcgaggc ccccagacgc tcgcgccggt cagcgcgaga tcagttgacg
     1021 agcggctccc cgtcgctcgc gggtgccggc ccgacggtga ccgtccgctc ctcggtgccg
     1081 cggacgagcg tcacctcgag cggaagcccc gtctcaccga gcacgtcatg gagatcgtcg
     1141 atgtcgggga tcggcttgcc gcccgcggcg acgagcaggt ccccctcgag gatcccggcc
     1201 gcctccgcgg ggctgcccgc ctcgacgccg cgcaccagca ctccgtcgag ctcgggcagg
     1261 ccgaccgagc gccggagccg ccgcgcaacg tgagccggcg cgaccgcgat cccgaggtgg
     1321 ggccgctcgc gggcctcgcc gcggccgagg gcgtctaccc gcgacttgag gccggcgtcg
     1381 gccggcagcg ccaggtagaa accctcgccg acccgattgg tgttcaggcc ggcgagcgcg
     1441 ccgtcggcgt cgagcagtgg gctgccggac gaacccgggg cgagcggcgc cgtgtgctcg
     1501 aacgagccgg agatccgccg gccaccgggg ccgcggaacg ctcgctcgac ggacgagacg
     1561 aacccgaacg agacgcgcgc cccgcctgac ggggtggccg cggccgcgaa cacggcggaa
     1621 ccgatggtga cgttgccgga cgaccaggcg atcggcgggg cgccggcggt gtcgacctcg
     1681 accacggcca ggtcgccgtc ccagtcgacg cccttgacgg tgccgaccgc ggttcggccg
     1741 cccgggaagc gcacggtgac ctcgccgccg cgcaggttgt gggcgttggt caggacgtga
     1801 ccgtcggcga cgacgacgcc tgagccacgc agccgggagc cgatcccgac gacggagggg
     1861 ccgactgagg cggccaccgc ggcgacggcc tcctggagct cgttgagcac ggacatgagc
     1921 gatggaacct cgggactgcg ttgcgtgact cacgggtgag ccagtgactt gcgatttgca
     1981 atctactact ggccacggcg tcccgcaagt ccctcgccta ccatgggggc cgatgagcag
     2041 ccctgtcgac gcctctgccc acgtcccggc cgcggccagc gccctgggcg tcgcgctcga
     2101 ccgggtcggc gaccgctgga gcctcctgat cgtcgaggcg ctgctgccgg gcgcgcggcg
     2161 gttcggcgag ctcggcgagt cggtcaccgg gatcgccccc aacatcctct ccgaccggct
     2221 gcgccgcctc gagcgggccg gcatcctcgt cgccacgccg tacagccggc gccccgtccg
     2281 gatggagtac gcgctgaccg ccgacggccg cgagctcgcc ggcgccctgc atctgctcgc
     2341 ggactggggc gtgcgtcgcg gcgggtctgg cgagccgctc cggcacgacg tctgcggcac
     2401 gcccctcgag gcccgctggc actgcccgac ctgcggccgg ttggtcgagg acgacgagcc
     2461 cgacggcctg cgccgcctct agggcacccc ggcgcccggc gagccgccgg gactcagaac
     2521 ccgagcgggt cgaacgcctc ggtccgctca gcgacggcct cggccccgag ccgtcccgag
     2581 gcgaagccga gcgtctcgtc cacgagcgtg ccccagccgc cctcctcgag caagcggagg
     2641 gtcgtgttcg cggcggccac cgctcggttc gcgtggtcca gggccatccc gcgcagctcc
     2701 ggcgtctcac gggacgcccg caggctcgcg gcgaccgacg tcgcgccccg ggattgggcc
     2761 gtctcgggtg ccgaggcccc gggccggcgc acgatcgcga gcgcgtcgcc cgcatcgggc
     2821 agcagggcgg cgacgatcgc gtgccacatc gtcgcgagat cgttgcgcag cggcggttcg
     2881 acgaacgcga gggggtggcc cggcagcagc gcgcgccgga gggcgacctc ggcggccacg
     2941 cgcgccggcg catcggactc ctcgaccagc cagtcgggga acgcaccgag cacgacggcg
     3001 tcgtcgggca gcccgtcgcg gcgggcgagc gccatggcca gcagctggag cgcgagcgcg
     3061 cggccggccc tcgtggccgg gtccgacggc acgccggacg acaggtcgcg agccacgatc
     3121 agcggccccg ccgggacgag gatgcgggtg cccgcgcggc cgaggagccg gtgggcgaag
     3181 acgtggtcgg cgatcgcgcg gtcggggtcg acgcgcccgg cgacgatctc gcgcatcggg
     3241 tccgcgatga cgatgtcgat ccgctcgaag gcggcgacca cggcctggtc gggcgcggcg
     3301 agggcctggg cgtcggtcat gatccggatg tagccgcgac gacgcgcgcc ggcctcgtcg
     3361 acgaaccggc gcaggaccgc gagcgagcgc tgggcgccgg tggggatcgg ctggcccgag
     3421 ggatcgaagt tgtcgagccc gccgcgcgac gagggctgcg cccgccagcg ttccaccggc
     3481 gcggcggtcc gcgcggtccg ctccgcgagc tcgcggctgg ccgggacgtc gacgcgcacg
     3541 agctcggccc cgccgtcgat cgccgcccgc gcctcgtcaa gggcgtccac gattgccgcc
     3601 gacccgaggc ccgtgccgag ccacggccgc gggggatcgc cgaggacggc gagcagctcg
     3661 cgccgcgccg tgcggttggc gtcgatccgg tccatcgcgg cccgcgacag cgccgacgcg
     3721 tcggccagcg cgatcgcccg ccgatcgggc tcggcgagga gctcggcctc gagcccgagg
     3781 tccacatcgc ccgccgcgac cgcgaggccc agctcctgcg gggcgaggtc gtactcggcc
     3841 agcgccatcg cgaacggcag cgcgatccca ccgccgaggc ggcgcgggtc ggggccgaga
     3901 tagcggtcga cgacctcggc ggcgagcggg cggccggcgc ggtcgacccc ggtgaccccg
     3961 aacaggcgca ggatcgcgcg ctcctgcccg atcgtcgtcg acgacgccgc gcccgagccc
     4021 caggcgaccg cgagcccgtt ggcgcgcgcg agcagggcct caaggtccga tcccgccgcg
     4081 agctgctgca tgcccgcagt atggctggcg ccgccggggc ggcgcgaacc ggcggtcagc
     4141 ctcgcgcctc ggtcagcggg cgggcggctc cgccgacgtg cgcgcggtgt gcccgatcca
     4201 cacgtggccg tcggtgaacc gctcggcctt ccagatcggc gcccgcgcct tggtctcgtc
     4261 gatcgcgtag cgggccgcgg cgaacgcggc gtcccggtgc ggcgcaacgg cgacgatcac
     4321 gaccgatgcg tcacccagcg gcaccgcccc cgatcggtgg acgatcgcga ggcgccggac
     4381 cccgaagcgg gcctcgacct catccgcgat ctcgccgagc accgcgatgg ccatcgattc
     4441 gaacgtctcg tactcgaggc catcgacctt ccggcccgcg tgccgggccg cctcgtcctc
     4501 ctggccgggt gccggcgtcc ccggggtcga gcgcgtgcgg ccggtgaacg cgaccacgcc
     4561 cccgtcctcg tccgtcgcga ggcgcgcggt cagcgccgcc acgatgtcgg ccgcaaacgg
     4621 ctccgcatgg atctcgagga tccgcgcggc cacgtccccc gacccgccgc tgaccggcgg
     4681 gatgcaggcg agctcatcgc cgtcggcgag ggtggtgtcc ggatcggcgt acgtgccgtt
     4741 gacggcgaag cgcagcgacg agcgcccggc ggcgagagcg ggcaccgacc ccgcgacggc
     4801 ggtccaggcg tcgtccacgg tcgacccaac cggcacctcg agccgcagct cgcgcatgcc
     4861 cgcgatctcg cgctgcatcg cgaacaggcg gacgcggatc cgcagcgcgg gcaacagccg
     4921 gtcggtcgcc atcagacgag cagcccggcg ccgaagtaga ggccggcggc gaggatcgtg
     4981 cccgcggcga tcgccccgac gtcgatgtac aggaggccgg tcgccgactc catcgaccgc
     5041 gtacgggcgg tctggaccgc cgcccaggac acgatcagcg ggaacaccag cccgatcacc
     5101 aggcgcagcc acacgaacag cgcccacggt ccgaccaggg ccgagaacgg cccaccgccg
     5161 gtcgccgccg aacccggacc cgccccgaag cccacccagc ccacgaacag cacgacctgg
     5221 agcgcgacga tccacagcag ccggcgcgag accatcacga ggggcgcctc aggcagccgc
     5281 ggggtgacga gataccagtg tccgaggatc atcgccgcga acacgccacc cgtcgccgcc
     5341 gcgagcaccg tgagctggat cgccagcggc acgacaccca gcgggctgcc gccccacgtc
     5401 agcgccccga gcaggatcac tgccaggccc gcgacgaggc ccgcgatcgc cggcaggacg
     5461 gcgcgccgac cgcgcgccag cgccacggtc gtcacgaccg cgagcaggac gaacgccacg
     5521 agtgcggcac gccggggctc gtcgaacgcc gcgttcaccg ggatcggcga cccggatccg
     5581 acggccggca gccccgtgtc ggacaggtag gccagcacgc cgaagcccgc ggcacacgcg
     5641 gccgtgaacg cgaggtagcc cttggtggcc ggggtcgcca gccgcgccag cacgacgacc
     5701 gcgaacgagc cgacggcgag gccggtcagc agcgtccagt tgatcagcgc gaggttggtg
     5761 gcgatctgct cggcgggcac gggcggagtg tagccgcgac cccgcccgag ggccgacgga
     5821 tcggccggcg agcgtcggat aatgaccgcc gggaatgcac gcaggcgccg cggcacggca
     5881 ggaggcttcg atggcgatcg gtcgggacac cacgttcacc tggctcggcc atgccggcat
     5941 cgagatcgcg acgcccggcg gccaggcgct actgatcgac ccctggttcg gcaatccgaa
     6001 cagcccgcgg gccgtggccg accaggagcg ggccgacgtg ctgctggtca cgcacggcca
     6061 cttcgatcac ttcgacgcgg acacgctgga tctcgctcgc cggctgcacc cgaccatgcc
     6121 ctgcgtccac gagctcagcc tctacctgat cgccgagctg ggcgacgccg ccggggtgat
     6181 cgggatgaac gccggcggga ccgtcgaagc gaacggcctc aaaatcacga tggtgcccgc
     6241 ggcgcactcg agcggcgacc tgatgggtgg cgatgccgtg cgctacttcg gcgaggcggt
     6301 gggcttcgtg atcgagctcg agaacgggtt ccgggtctac gactcgggcg acacgaccgt
     6361 attcggcgac atggccctga tccgcgagct ctaccggccc gacctcgcca tcctgccgat
     6421 cggcggccac ttcacgatgg acccggcggc ggcggcggtc gccgtgggcc tgctcggcgt
     6481 gcgcgacgtc ctgcccgtcc actggggaac gttcccgatc ctggcgggga cgccggcggc
     6541 cctggcggcg gagctggccg cgagggagat cgtggcgac
//
LOCUS       DBNHXO010000185        12721 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_247222, whole genome shotgun sequence.
ACCESSION   DBNHXO010000185 DBNHXO010000000
VERSION     DBNHXO010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 12721)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12721)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12721
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_247222"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="AC162A_03920"
     CDS             complement(<1..734)
                     /locus_tag="AC162A_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5010017.1"
                     /translation="MISRPLLGQTWRAQRTRVAIISVALVVWSSLMPVIYATFGRQME
                     SLIESGIIPEAFMRLMGSSVFGLDAAIALGVSHPIAIALQVVYPVGFAALAIAGERQN
                     GTLEVLLSRPVSRRAVFLTLLLAILGIAALTTAATVAGTLIGSAAYGLTGDLSAGNEA
                     FLYVNLVFLLAAFAGIALAASASFDRPGPAIGIGLAVVLVGYLLEILGQLWPDAQGLQ
                     PISPFHYMRPFEILAGRGQASDLVVLL"
     gene            complement(731..1666)
                     /locus_tag="AC162A_03925"
     CDS             complement(731..1666)
                     /locus_tag="AC162A_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009487596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5010018.1"
                     /translation="MNAISTIGLSKTYGGGRRPRHGGGAVRALDDVTLDVREGEVFGF
                     LGPNGAGKSTFIRLLLGFLHPTAGSARVLGLDSTRDSVAIRSRIGYLPGGIAFHDGLT
                     GERHLDDLADLAGRPSVRRAALCDRLELADRTLRRPIRDYSRGMRQKLGIVQALQHDP
                     ELAILDEPSEGLDPLMQRAFYGILDDLHEAGRTVLFSSHVLSEVERVCDRVAVVRAGQ
                     LVANEDVGALLARRKRRVELQFEGDPPDLTGVPNVTEVVIVDGGLTCSLAGDPRPLLN
                     AVASLAINDLLIEPARLEDAFLEFYAADDSPDLAS"
     gene            1730..2401
                     /locus_tag="AC162A_03930"
     CDS             1730..2401
                     /locus_tag="AC162A_03930"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIR5010019.1"
                     /translation="MLYRADPWLHVIAGCMSSGKTDELLRLLRRAEIARRRILLVRPD
                     VDDRTPSEYAESRSKARYPSVLVPRSKPVEILSLARERDVDLVGIEEAQFFDPSLVPT
                     VETLRQSGRHVIASGLNTDFAGRPFGAMPELLALADDITMLSAICVVCGETATRTQRL
                     VGGRPAAIDDPLIVIGGFGGPAIETYEARCLRHHEVAPAGSARGVVADDGPARVADPA
                     VAGEG"
     gene            2747..3223
                     /locus_tag="AC162A_03935"
     CDS             2747..3223
                     /locus_tag="AC162A_03935"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006370680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5010020.1"
                     /translation="MPKLSAGLLLYRHAGDGVEVLVAHPGGPIWARRDTGAWSLPKGA
                     PLDGEGLLETARREFHEETGHEAPDAPTIDLGEVRMRSGKVVHAWAVEGDLDPARLQS
                     MTAEIEWPPRSGRRLHVPEIDRIDWVAPAEARRRLNPAQATFVDRLLQALARAPSA"
     gene            3282..3665
                     /gene="mscL"
                     /locus_tag="AC162A_03940"
     CDS             3282..3665
                     /gene="mscL"
                     /locus_tag="AC162A_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR5010021.1"
                     /translation="MLGEFKAFLTKSNALALAIGVIIGAALGSVVNSLVNDIIMPPIG
                     YALGGVDFNQLAYVLQPAVNGDPATEVAIRWGAFIQTLITFVIIAFVVFWIAKLLVRE
                     PAPEDPEPSDEVKLLTEIRDELRKT"
     gene            4068..4430
                     /locus_tag="AC162A_03945"
     CDS             4068..4430
                     /locus_tag="AC162A_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5010022.1"
                     /translation="MQRSSISQQTPPRSPRRRSAATSPEWRRALGDELRRLRTGRKLT
                     QAAFGTPLTRAFVSAVERGRAVPSIPALEVMLGRVGTPMSEFFAAIEARLVDRDLTAE
                     YDPQRGNHRYPSAASRRR"
     gene            4387..5679
                     /locus_tag="AC162A_03950"
     CDS             4387..5679
                     /locus_tag="AC162A_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5010023.1"
                     /translation="METTVTRRRRRVVVDQRDLARAIGGRIRAARLTAGMTQQELAGD
                     RYTKAYVSALELGHAKPSMAALDYLAPRLGTTSDRLIADHASRWSRVDADLHLAAGRL
                     PEAAEAYEDLAAGSVDRVSRGELLLGAAEAHCKLNHAQQAAPLLVEAIALLADGGRQA
                     DQKRAQYWLSYVHMALDDPEEARRLLLDMLGSDAEATEDPDFEVRVRIALAQVETEHG
                     DPARAALYLEEARELAAGLDLRKRGIYFDALSKARFAANDTEGAIRAATEALALFRAS
                     EQEVQEAMLENALAMSFVRLGNLAKAGELAAQAVAIAERLHHFQTLGHYLDTQATISL
                     ARGDLADALRLTDRALAIEAEYGPANDQVGARITRAQALTAAGRTVEAEAAWADAAGV
                     ARQLSSPLRRRRIFAAWAESLAAQGRHAEAYEVMRQAL"
     gene            5774..8536
                     /locus_tag="AC162A_03955"
     CDS             5774..8536
                     /locus_tag="AC162A_03955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR5010024.1"
                     /translation="MNYPYPAPAPAPKPRPKRRGIGPAATMGLVLFAALALAGVAGAG
                     AALAMFASISSDLPEPSVLERIELPEQSVVLDRTGKIELARFGEFNREVVTFADIPPV
                     LVDATTAVEDGSFWDNAGFDTIGIVAAALDSLRGQPRGASTITQQLVRQRLLSENGSA
                     QTELSATRKLREIIQSIRVTQAYPGVDGKQRIMAAYLNQNYYGNESYGVAAAAKSYFG
                     VGLKDLTLAQAAILAALPKSPSTYDLVQNAEIVCLDPGAQESEADECKESELVVPPDA
                     PIVLRRNLVLDLMEKGRTPLTGTTFSADDFDAARAEPVILAAQQSSQWKAPQFVWQVR
                     KELTERLCGDDTETCTVIERGGLTVTTTLDMRLQGIAEKWVKAATVVPNAKNPRATAR
                     ALGLPYEQWMQNLVSKDLHNGALVAMDYETGDLVAYVGSADATARRATKKFQPRFDVL
                     ADGWRQPGSAFKPVVYSTGIAQRSITAATMFMDVVTNFGGGYTPTDADNLERGPVRVR
                     DALSFSLNIPAVKAMAVIGNPNVQAQAEAMGIQFLDGQVDAGLSFALGVEEVHPIDLI
                     RAYGVLGDKGRIVDQTTLRTVVDNNGKALVEESQRPKPQQVLDEGAAFITTDILAANT
                     NPNKNPFWGQFAIDEGGRRRPATLKTGTSNEARDLNAYGYIGSPRNAARKDGEFALAV
                     GAWNGNSDNSLVARPGTTLFSIEVTTHVWQGFLEEATKGWTINGFGAPNGLEVVVIDP
                     WTGLLASPGGKAVEELFLPGTAPTSVLPKEARCGEAVLSNAGFEDEHAAWMAADHGWL
                     IRARKGPGRRGGPEDTATAYFYNPLWNPYGRSWGAITGGPGCDEPSESPSASVDPCAS
                     PGGILDPSASPGVICPSAEPPTEPPTETPTEPPTEPPTPEPTPPPTPEPTPPPTPEPP
                     SEAPPS"
     gene            8663..9850
                     /locus_tag="AC162A_03960"
     CDS             8663..9850
                     /locus_tag="AC162A_03960"
                     /inference="COORDINATES: protein motif:HMM:NF022100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COG1470 family protein"
                     /protein_id="HIR5010025.1"
                     /translation="MSDRVSGRPSRLQPAARAALLLGLLGIVAGPLASLATAAGGLTV
                     TTPFPAVVAEPGSTASFALTVDVPSAMRVDLKTTGVPTGWTARFRGGGLTVDGVYVDP
                     KASPSLTLDVEIPDSATASSNTIVVTATGGGLSDALPLSIRIADAAAGNVTLTTDSPG
                     QRGPASTTFSYTITIHNDTAAEIAFTMDAVGPPGWTVAAKPSSAAQATSVTVTPSGTG
                     TMSVTATPPSDVTAGDYPLQASVTGGGKTASIDLAVSITGTYALVLTTPDQVLSTTAN
                     AGSVKDLQLTVTNNGSAPVANVAMSASAPTNWKVEFTPPTVPSIEAGATQTVTAHITP
                     SGDAIAGDYEMTITSRATEASDDVSIRVRVETPQFWWIVGVVLIVAVFAGLYWVFRTY
                     GRR"
     gene            9856..11013
                     /locus_tag="AC162A_03965"
     CDS             9856..11013
                     /locus_tag="AC162A_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020753623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5010026.1"
                     /translation="MTDERTPPSDEGQGVPRRRARPRNTVGTGALSGPASGAVTTTER
                     PDVRADDEPRPRRRRTVADILAEVPEDAVIVARRLTKEYGDFTAVDSLDLTIRAGEVF
                     GLLGPNGAGKTTTVLMLLGLTEPTRGIVRVIGLDPTREPLAVKRKVGYLPDNVGFYGG
                     MTGRENLRYTAKLNGLPRSVAEARIDALLHEVRLDDAADKKAETYSRGMRQRLGIADA
                     LVKEPTVLILDEPTIAIDPAGVEELLALLRRLVTERNLTVLLSSHILGQVQSLCDQVG
                     FFAAGRLVAAGPLEELARGTDQIVELEVAIDGDPALIDPVAAAVPGVRSVTTDPRNER
                     LRVVTATSDVAGPLAAAIAGAGLSIVHLRRRGSDLLELYRRYVPEAADGRR"
     gene            11000..12028
                     /locus_tag="AC162A_03970"
     CDS             11000..12028
                     /locus_tag="AC162A_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5010027.1"
                     /translation="MAAADSRRSDAVRSARRSERAPSGGWRTIAGKEFADHLGSIRFF
                     VLLVVIAIAAIVPMYFVSTDISSAAPQLAGSPAVFLALFVVGSESVGGVTTVAFAALL
                     VPLVGIAFGFDGINSERSQGTLSRLLAQPIHRDDVVNGKFAAGIAVITVMLAALVVLV
                     TALGIVRLGIIPSVEELARVVVWLLSTVLYASFWLAFALLLSVVIRGAASAALVGFGS
                     WLALILFGQFLLPIVANVLFPPSTTTFQGYAYDSTQSAAVFLRISPAVLYQDIVRAVM
                     NPTTTSILGFANIGQAISDAERSQATLLTLDQSILLVWPQIVILIALTTAMFALAYVL
                     FLRQEVRA"
     gene            12080..>12721
                     /locus_tag="AC162A_03975"
     CDS             12080..>12721
                     /locus_tag="AC162A_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR5010028.1"
                     /translation="MSQPTPLPATAPGAPRGLAALAGSVAALVALAVGELVAGLIAGV
                     PSPIAAVGAAIIDFAPPGSKDLMVSLFGTNDKVALTVFVVAVVLLVGAGVGLLARRHH
                     GIAIVVILAIVGVGLLASLRLAGSSTTLVLVSAAAQAGLAILVLDALMGSARSAGAAG
                     TSPARRSFLLRAGVLGTLAIVGGGLGRRMLEGRAQQATAAAIEIPGATDPVAAP"
BASE COUNT         1857 a         4715 c         4390 g         1759 t
ORIGIN      
        1 agcagcacga cgaggtccga ggcctgccct cgaccggcga ggatctcgaa cggccgcatg
       61 tagtggaacg gcgagatcgg ttgcagaccc tgggcgtcgg gccagagctg cccgaggatc
      121 tcgagcaggt atccgaccag gacgacggcc aagccgatcc cgatcgccgg acccgggcgg
      181 tcgaacgacg ccgacgccgc gagggcgatc cccgcgaagg ccgccagcag gaagaccagg
      241 ttgacgtaca ggaacgcctc gttcccggcg ctgaggtcgc cggtcagccc atacgcggcg
      301 gagccgatca gcgtcccggc gaccgttgct gccgtggtga gggcggcgat cccgaggatc
      361 gcgagcagga gggtcaggaa cacggcgcgc cgggaaaccg gtcgcgacag gagcacctcg
      421 agggtgccgt tctggcgctc gcccgcgatc gccagggccg cgaatcccac cggatacaca
      481 acctggaggg cgatggcgat cgggtggctg acgccgagtg cgatggccgc gtcgaggccg
      541 aacacgctcg accccatcag gcgcatgaac gcttccggga tgatgccgga ctcgatcagc
      601 gactccatct gccgcccgaa cgtcgcgtag atgaccggca tcagcgatga ccacacgacg
      661 agcgcgacgc tgatgatcgc gactcgagtg cgctgggcac gccaggtctg gccgaggagc
      721 ggacggctga tcacgaagcg aggtccgggc tgtcgtcggc ggcgtagaac tcgaggaagg
      781 cgtcctcgag ccgcgccggc tcgatcagga ggtcattgat cgcgagggac gccacggcgt
      841 tgagcagcgg cctggggtcg cccgcgaggg agcaggtcag gccgccgtcg acgatgacga
      901 cctcggtgac gttgggcacc ccggtgaggt ccggagggtc cccctcgaac tggagctcga
      961 cgcggcgctt gcggcgggcg aggagcgcgc cgacgtcctc gttggcgacc agctgacctg
     1021 cccgcacgac cgccacccga tcgcagacgc gctcgacctc ggacaggacg tgggacgaga
     1081 acagcaccgt ccggcccgcc tcgtgcaggt cgtcgaggat cccatagaag gcgcgctgca
     1141 tcagcggatc gaggccctcc gacggttcgt cgaggatcgc gagctccggg tcgtgctgga
     1201 gggcctgaac gatgcccagc ttctggcgca tgccgcgcga gtagtcgcgg atgggtcgcc
     1261 ggagcgtccg atcggccagc tccagtcggt cgcacaacgc cgcgcgccga acggacgggc
     1321 gacccgcgag gtcggccagg tcgtcgaggt gccgttcgcc ggtcagccca tcgtggaagg
     1381 cgatgccgcc gggcagatag ccgattcgcg agcggatggc cacggagtcc cgggtgctgt
     1441 cgaggccgag gactcgggcg ctcccggcgg tcgggtggag gaacccgagc agcaggcgga
     1501 tgaacgtgct cttccccgcc ccgttgggcc ccaggaagcc gaagacctcg ccctcgcgga
     1561 cgtccagcgt cacgtcgtcg agcgccctga ccgcgccgcc gccgtgcctg ggccgccggc
     1621 cacccccata ggtcttcgac agcccgatcg tcgagatcgc gttcatcgcg ccgatcctaa
     1681 cccggccggc atggcgccga tcgtgccccg acccggtacg atcgccccga tgctctatcg
     1741 cgccgacccg tggctccacg tcatcgccgg ctgcatgtcg tcgggcaaga ccgacgagct
     1801 gctgcggctc ctgcgacggg ccgagatcgc gcgccggcgg atcctcctcg tccgccccga
     1861 cgtcgacgac cggacgccca gcgagtacgc ggagagccgg agcaaggcgc ggtatccgtc
     1921 ggtgctcgtc ccgcgcagca agccggtcga gatcctctcg ctcgcccgcg agcgcgacgt
     1981 cgacctcgtc ggcatcgagg aggcgcagtt cttcgacccg agcctcgtgc ccacggtcga
     2041 gaccctgcgt cagagcgggc gccacgtcat cgcgagcggc ctcaacaccg acttcgccgg
     2101 ccgcccgttc ggcgcgatgc cggagctgct cgccctggcc gacgacatca cgatgctcag
     2161 cgcgatctgc gtcgtgtgcg gcgagaccgc cacccggacg cagcgcctgg tcggcggccg
     2221 gccggcggcg atcgacgacc cgttgatcgt gatcggcggg ttcggcggtc cggcgatcga
     2281 gacctacgag gcgcgctgcc tgcgccatca cgaggtcgcc ccggcgggga gcgcgcgggg
     2341 cgtggtcgcg gacgacggtc cggcccgggt cgccgacccg gcggttgcgg gcgagggctg
     2401 acggccccga aacgccactc atccacaact tgtccacgtc ggccccgaat cgtggacaac
     2461 tcgcggcacc ggcggccgaa gttcgtggac aagcccgttg ttgcgcccgg gacccgccgg
     2521 atatcgttcg gtctgcccga aggggccggt ggcaccgacg ccggactggt gagcaatcat
     2581 cggaccgggt ccccggaccg ccgccacgac gaccgcaagg ccgacgaggt ggctggagca
     2641 cggggccgct gcgacaaggc cgagcttgct cggcagggtc gcgggcgtga cggtgcggta
     2701 cggttccttc gggcgctttc gtgtgcgccg aacgtcggat cccgccatgc ccaagctctc
     2761 cgccggtctc ctcctctatc ggcacgccgg cgacggcgtc gaggtcctcg tcgcccaccc
     2821 gggtggtccc atctgggctc gtcgcgacac gggcgcgtgg tcgctgccca agggcgcgcc
     2881 gctcgatggg gagggcctcc tcgagaccgc ccggcgcgag ttccacgagg aaacgggcca
     2941 cgaggcgccg gacgccccga cgatcgatct gggcgaggtc cggatgcggt cgggcaaggt
     3001 cgtccacgcc tgggcggtcg agggcgatct cgacccggcc cgtctccaga gcatgaccgc
     3061 ggagatcgag tggccgcccc gatccggacg ccggctccac gtcccggaga tcgaccggat
     3121 cgactgggtc gcgccggccg aggctcggcg ccgcctcaac ccggcccagg ccaccttcgt
     3181 cgaccgcctc ctccaggccc tcgcgcgcgc gccctcggcg tgacgtatcg tcgggaccag
     3241 ccggtcgccg ctgccggcca cggggacgga ggcacggtca catgctcggt gagttcaagg
     3301 cgttcctgac caagtcgaac gcgctcgcgc tggcgatcgg cgtgatcatc ggcgccgcgc
     3361 tcggctcggt cgtcaactcg ctggtcaacg acatcatcat gccgccgatc ggctacgccc
     3421 tcggcggggt cgacttcaac cagctcgcgt acgtgctcca gccggcggtc aacggcgatc
     3481 cggccaccga ggtcgcgatc cgctggggcg ccttcatcca gaccctcatc acgttcgtga
     3541 tcatcgcgtt cgtggtgttc tggatcgcga agctgctggt tcgcgagccc gcgcccgagg
     3601 accccgagcc atccgacgag gtcaagctcc tcacggagat ccgggacgag ctccgcaaga
     3661 cgtaaggcga aagtgccgcg gcgccaggtc tccgcgtctc tcgcacaaac agagagccgc
     3721 cccgcctcgg cagcggtgcg gctctcgggt tccccccatc tggctcgact ggtcacccgt
     3781 cgagccgggg gttcccggta ctccccccgc ggttgtaccg ggcccgcgga tcatacgcgg
     3841 tcccttcgcg cttgtcactg gtttattgac ttatcgaggg aagttcccgc cgccgccatc
     3901 ggcgagcgcg acagccaccg agatcatgct cagggcgaac gccatggcca gcgagacacc
     3961 caacttcagt ccacgcatcg tcatcccacc tcgggtggtg cggatcggag ggccggccag
     4021 gtggtcgtgc ttgcccccgc cgctgccaca atgctaggct cgactccgtg cagcggtcaa
     4081 gtattagtca acaaacgcca ccacgctccc cgcgcagacg ctcggcagcg acgagtccgg
     4141 agtggcggcg cgcactcggc gacgagctgc ggcgccttcg aaccggccgg aagctcaccc
     4201 aggccgcgtt cggcacgcca ctcacgcgcg cgttcgtctc ggccgtcgag cggggccgcg
     4261 ccgtcccctc gatcccggct ctcgaggtga tgctcggacg cgtcggcacg ccgatgtccg
     4321 agttcttcgc ggcaatcgag gccagactgg tcgaccggga cttgaccgcc gagtatgatc
     4381 cgcagcgtgg aaaccaccgt tacccgtcgg cggcgtcgcg tcgtcgttga tcagcgcgac
     4441 ctagcacggg ctatcggggg ccgcatccgt gccgcgcgtc tcaccgccgg catgacccag
     4501 caggagctgg caggagaccg gtacacgaag gcgtacgtca gcgccctcga gctcgggcac
     4561 gccaaaccgt cgatggccgc cctcgactat ctcgctcccc ggctgggcac gacgtcggat
     4621 cgcctgatcg ccgaccacgc gtcccgctgg agccgcgtgg atgccgacct ccatctcgct
     4681 gccggccggc tccccgaggc tgccgaggcg tacgaggacc tggctgcggg ctcggtcgac
     4741 cgcgtgtcgc gcggcgagct gctcctcggc gcggccgagg ctcactgcaa gctcaaccac
     4801 gcccagcagg ccgcgccgct gctggtcgag gccatcgcgc tgctcgcgga tggcggcagg
     4861 caggccgacc agaagcgcgc gcagtactgg ctctcgtatg tgcacatggc gctcgacgat
     4921 cccgaggagg cccgccgcct cctgctggac atgctcggct ccgacgccga ggcgaccgag
     4981 gaccccgact tcgaggtccg ggtccgcatc gcgctggcgc aggtcgagac cgagcacggc
     5041 gacccggcgc gtgcggcgct gtatctcgag gaggcgcgcg agctcgccgc cggcctggac
     5101 ctccgcaagc gcggcatcta cttcgacgcc ctctcgaagg cacggttcgc cgccaacgac
     5161 accgaggggg cgatccgggc cgcgaccgag gccctcgcac tgttccgcgc ctccgagcag
     5221 gaggtccagg aggcgatgct cgagaacgca ctcgccatgt cgttcgtccg cctcggcaac
     5281 ctcgccaagg ccggggagct ggcggcgcag gcggtcgcga tcgcggagcg gctgcaccac
     5341 ttccagacgc tcggccacta cctcgacacc caggcgacga tcagcctcgc gcgcggcgac
     5401 ctcgcggacg ccctgcggct gacggatcgc gcgttggcga tcgaggccga gtacgggcca
     5461 gcgaacgacc aggtgggggc ccggatcacc cgcgcccagg ccttgaccgc ggcgggccgg
     5521 accgttgagg ccgaggcggc ctgggccgac gccgcgggcg tcgcccgcca gctgtcgtcg
     5581 cccctgcgcc gacggcgtat cttcgcggcg tgggccgagt cgctcgcggc ccagggccgc
     5641 catgccgagg cctacgaggt gatgcgccag gcgctctgag cgcggtcacg gccggctcga
     5701 cggcccctcg cgacacccgc gggccgtccg cgccggcagc acgaagtcgc cacccacggc
     5761 agactctgcg ccgatgaact atccgtatcc ggcgccggca ccggccccca agccgcgccc
     5821 gaagcgccgc gggatcggcc ccgcggcgac catgggcctc gtgctgttcg ccgcgctcgc
     5881 gctcgccggc gtggccggtg cgggcgctgc gctcgccatg ttcgcctcga tcagcagcga
     5941 cctcccggag ccgtcggtcc tggagcggat cgagctgccc gagcaatccg tggtcctcga
     6001 ccgcaccggc aagatcgagc tcgcccggtt cggggagttc aaccgcgagg tcgtcacgtt
     6061 cgcggacatc ccgccggtcc tcgtcgacgc gaccacggcc gtcgaggacg ggtcgttctg
     6121 ggacaacgcg ggcttcgaca ccatcggaat cgtggccgcg gccctcgact cgctgcgggg
     6181 ccagccgcgc ggcgcatcga ccattaccca gcagctcgtg cgccagcgcc tgctgtccga
     6241 gaacggctcg gcccagaccg agctgtcggc gacccgcaag ctgcgcgaga tcatccagtc
     6301 gatccgggtc acccaggcct acccgggcgt cgacggcaag cagcggatca tggccgccta
     6361 cctcaaccag aactactacg gcaacgagtc gtatggcgtc gccgcggccg ccaagagcta
     6421 cttcggcgtg gggctcaagg acctcaccct cgcccaggcc gcgatcctgg ccgcgcttcc
     6481 caagtcgccg tcgacctacg acctcgtcca gaacgccgag atcgtgtgcc tcgacccggg
     6541 cgcgcaggag tcggaggcgg acgagtgcaa ggaatcggag ctcgtcgtcc ccccggacgc
     6601 ccccatcgtc ctgcgccgca acctcgtcct ggacctcatg gagaaggggc ggacgccgct
     6661 cacgggcacc acgttcagcg ccgacgactt cgacgccgct cgcgcggagc cggtcatcct
     6721 ggccgcgcag cagtcgagcc aatggaaggc cccccagttc gtgtggcagg tgcgcaagga
     6781 gctgaccgag cggctgtgcg gcgacgacac cgagacgtgc accgtgatcg agcggggcgg
     6841 gctgaccgtc acgaccacgc tcgacatgcg cctccagggc atcgccgaga agtgggtcaa
     6901 ggcggcgacg gtcgtcccca acgcgaagaa cccgcgggcg accgcgaggg cgctcgggct
     6961 gccctacgaa cagtggatgc agaacctcgt cagcaaggac ctccacaacg gcgccctggt
     7021 ggcgatggac tacgagaccg gtgatctcgt ggcctatgtg gggtcggcgg acgccaccgc
     7081 ccggcgggcg accaagaagt tccagccgcg cttcgacgtg ctcgccgacg gctggcggca
     7141 gccgggatcc gcgttcaagc cggtcgtgta ctccacgggg atcgcccagc gcagcatcac
     7201 ggcggcgacg atgttcatgg acgtcgtcac caacttcggc ggcggctaca cgccgaccga
     7261 cgcggacaac ctcgagcgcg gcccggtgcg ggtccgtgac gcgctcagct tctcgctcaa
     7321 catcccggcc gtgaaggcga tggcggtgat cggcaatccc aacgtccagg cgcaggccga
     7381 ggcgatgggg atccagttcc tggacgggca ggtcgatgcg gggctgtcgt tcgcgctggg
     7441 cgtcgaggag gtccacccga tcgacctgat ccgcgcctac ggcgtgctgg gcgacaaggg
     7501 ccgcatcgtc gaccagacga cgctccgcac cgtcgtcgac aacaacggca aggcgctggt
     7561 cgaggagagc cagcgcccga agccgcagca ggtgctcgac gagggcgccg ccttcatcac
     7621 caccgacatc ctggccgcca acacgaaccc caacaagaac ccgttctggg gacagttcgc
     7681 gatcgacgaa ggcggccgcc gccgcccagc cacgctcaag accggcacca gcaacgaggc
     7741 gcgcgacctc aatgcctacg gctacatcgg gtcgccgcgc aatgcggccc gcaaggacgg
     7801 cgagttcgcg ctcgccgttg gtgcctggaa cggcaactcc gacaactcgc tggtcgcccg
     7861 gcccggcacc acgctgttct cgatcgaggt cacgacccac gtctggcagg gattcctgga
     7921 ggaggcgacg aagggctgga cgatcaacgg gttcggggcg ccgaacgggc tcgaggtggt
     7981 cgtgatcgac ccctggaccg gcctgcttgc atcgccgggc ggcaaggcgg tcgaggagct
     8041 gttcctcccc ggcacggccc cgaccagcgt gctgcccaag gaggcgaggt gtggcgaggc
     8101 cgtgctgagc aacgcgggct tcgaggacga gcacgccgcc tggatggccg ccgaccacgg
     8161 ctggctgatc cgcgctcgca agggcccggg caggcgcggc ggtcccgagg acaccgcgac
     8221 cgcctacttc tacaacccgc tgtggaaccc gtatggccgt tcgtgggggg cgatcaccgg
     8281 cggccccggc tgcgatgagc cgagcgagag cccgtccgcg tccgtggacc cgtgcgcctc
     8341 accaggcgga atcctcgatc catcggcatc gccgggggtc atctgcccat cggccgagcc
     8401 gccgaccgag ccgcccacgg agactccgac cgagccgccg accgagccgc ccacgcccga
     8461 gccgacgccg ccgccaaccc cggagcccac gccgccaccg acgccggaac cgccatccga
     8521 ggccccgccg tcctgacgca gcgggcggtc tcagcacctt ctcaggattt cggcctatga
     8581 tccgcccggc cacgaccggt tcgagggacc gggctcgcga gccatcgtcg gacctgtcac
     8641 cgtcctgaac gcggagattc tcgtgtcgga tcgcgtttcg ggccgcccca gccggctcca
     8701 gccggcggcg agggccgccc tcctcctggg tcttctcggc atcgtcgccg ggccgctggc
     8761 atcactcgcc accgcggccg gtggactcac cgtcacgacg cccttccccg cggtcgtggc
     8821 cgagccgggc tcaaccgcct cgttcgcgct caccgtcgac gtcccgagcg cgatgcgcgt
     8881 cgacctcaag accacaggcg tgcccaccgg ctggaccgca cgcttccgag gcggcggcct
     8941 gaccgtcgac ggcgtctacg tcgatccgaa ggcgtcgccg agcctgacgc tcgacgtcga
     9001 gatccccgac agcgccaccg cgtcgtccaa cacgatcgtg gtgaccgcga ccggcggcgg
     9061 cctgtccgac gcgctgccgc tgtcgatccg catcgccgac gcggccgccg gcaacgtgac
     9121 gctcacgacg gactcgcccg ggcagagagg gccggccagc acgaccttca gctacacgat
     9181 caccatccac aacgacaccg ccgccgagat cgcgttcacg atggacgccg tcgggcctcc
     9241 cgggtggacc gtcgcggcca agccctcgag cgcggcccag gcgaccagcg tcaccgtcac
     9301 cccgagcggg accgggacga tgagcgtcac ggcgacgccg cccagcgatg tgaccgccgg
     9361 cgactatccg ctccaggcga gcgtcaccgg cggcggcaag accgcctcga tcgacctcgc
     9421 ggtgagcatc accggaacct acgcgctggt gctcacgacg cccgaccagg tcctgtcgac
     9481 cacggccaac gccggatccg tgaaagacct ccagctcacc gtgaccaaca acggctccgc
     9541 gccggtcgcg aacgtcgcga tgtcggcgag cgcgccgacc aactggaagg tcgagttcac
     9601 gccgccgacg gtgccgtcca tcgaggccgg cgcgacccag accgtgaccg cgcacatcac
     9661 gcccagcggc gacgcgatcg ctggcgacta cgagatgacg atcacctcca gggccacgga
     9721 ggccagcgac gacgtctcga tccgcgtgcg cgtggagacg ccgcagttct ggtggatcgt
     9781 tggagtcgtc ctgatcgtgg ccgtgttcgc cggcctgtac tgggtcttcc ggacgtacgg
     9841 gcggcggtag ccgagatgac cgacgagcgg acgcccccgt ccgacgaggg ccagggcgtg
     9901 cccaggcgcc gggcgcggcc ccggaacacg gtgggcaccg gagcgctgtc ggggccggca
     9961 tcgggcgccg tcacaacgac cgagcgaccc gacgttcggg cggacgacga gccgcgcccg
    10021 cgccggcgtc gcacggtcgc cgacatcctg gccgaggtcc ccgaggacgc ggtcatcgtg
    10081 gcgcggcggc tgaccaagga gtacggcgac ttcaccgcgg tcgactcgct cgacctcacg
    10141 atccgggccg gtgaggtctt cgggctgctc ggacccaacg gtgccggcaa gacgacgacc
    10201 gtcctgatgc tgctggggct caccgaaccg acccggggca tcgtccgggt catcggcctc
    10261 gacccgaccc gcgagccgct cgcggtcaag cgcaaggtcg gctacctgcc cgacaacgtc
    10321 ggcttctacg gcggcatgac cggccgcgag aacctgcgct acacggccaa gctcaacggg
    10381 ctgccccgct cggtcgccga ggcgcggatc gacgcgctgc tgcacgaggt tcgcctcgat
    10441 gacgcggccg acaagaaggc cgagacctac tcgcgcggca tgcgccagcg gctcggcatc
    10501 gccgacgccc tggtcaagga gccgaccgtc ctcatcctgg acgagccgac catcgcgatc
    10561 gatcctgccg gcgtcgagga gctgctcgcg ctgctgcggc ggctcgtcac cgaacgcaac
    10621 ctgacggtcc tgctctcgtc ccacatcctg ggccaggtcc agtcactgtg cgaccaggtc
    10681 gggttcttcg ccgccggacg gctcgtcgcc gccgggccgc tcgaggagct cgcccggggc
    10741 acggaccaga tcgtggagct cgaggtcgcc atcgacggtg accccgcgct catcgacccc
    10801 gtcgcggcgg ccgtgccggg cgtccgctcc gtgaccaccg atccacgcaa cgagcgtctg
    10861 cgcgtcgtca ccgcgacgag cgacgtcgcg ggcccgctcg ccgccgcgat cgcgggcgcc
    10921 ggcctgtcca tcgtccacct gcggcgacgc gggtccgacc tgctcgagct gtaccgccgc
    10981 tacgtcccgg aggctgccga tggccgccgc tgacagccgc cgctcggatg ccgttcgctc
    11041 ggcccggcgt tcggagcgcg ccccgtcggg tggctggcgc acgatcgccg gcaaggagtt
    11101 cgccgaccat ctgggcagca tccggttctt cgtgctgctc gttgtcatcg cgatcgccgc
    11161 gatcgtgccg atgtacttcg tgtccacgga catcagcagc gcggcgccgc agctggccgg
    11221 ctcgccggcc gtgttcctcg ccctgttcgt ggtcgggtcg gagagcgtcg gcggcgtgac
    11281 gaccgtcgcc ttcgccgccc tgctcgtgcc gctcgtgggc atcgccttcg ggttcgacgg
    11341 catcaacagc gagcgcagcc aggggacgct gtccaggctg ctcgcccagc cgatccatcg
    11401 cgacgacgtc gtcaacggca agttcgcggc cgggatcgcg gtgatcacgg tgatgctcgc
    11461 ggcgctcgtc gtgctggtca cggccctggg gatcgtgcgg ctcggaatca tcccgtccgt
    11521 cgaggagctc gcccgggtcg tcgtgtggct gctctcgacc gtgctctacg cgtcattctg
    11581 gcttgcgttc gcgctgctgc tgtcggtggt catccgcggc gcggcgtccg ccgcgctcgt
    11641 cggcttcggc agctggctcg ccctgatcct gttcggccag ttcctgctcc cgatcgtcgc
    11701 caacgtcctg ttcccgccga gcacgacgac gttccagggc tacgcgtacg actccacgca
    11761 gagcgcggcc gtcttcctgc gcatctcgcc ggctgtcctg taccaggaca tcgtgcgggc
    11821 ggtgatgaac cccacgacga cgtcgatcct cgggttcgcg aacatcggcc aggcgatctc
    11881 ggacgccgag cgctcgcagg cgaccctgct cacgctcgac cagagcatcc tgctcgtgtg
    11941 gccgcagatc gtgatcctga tcgcgctcac gaccgcgatg ttcgcgctcg cgtacgtgct
    12001 gttcctgcgc caggaggtcc gcgcgtaggc gccgacccgt cgtcacggga gcgtcaccca
    12061 cgagcctgat actcctcgca tgagccagcc cacccccctg cccgcaaccg cgcccggggc
    12121 gccccgcggc ctggccgcgc tcgccgggtc ggtcgccgcg ctcgtcgcgc tggccgtggg
    12181 cgagctggtc gcgggcctca tcgcgggcgt cccctccccg atcgccgcgg tgggcgcggc
    12241 gatcatcgac ttcgcgccgc cgggctcgaa ggacctgatg gtctccctgt tcgggaccaa
    12301 cgacaaggtg gccctgacgg tgttcgtcgt cgcggtcgtg ctcctcgtgg gcgccggcgt
    12361 cggcctcctc gcccgccggc accacggcat cgcgatcgtc gtgatcctcg cgatcgtcgg
    12421 cgttggcctg ctggcctcgc tccgcctggc cggcagctcg acgaccctgg tcctggtgtc
    12481 cgccgctgcc caggccgggc tggcgatcct cgtgctcgac gccctgatgg gctccgcccg
    12541 gtccgcgggc gccgccggca cctccccggc gcgccgctcg ttcctgctgc gggccggcgt
    12601 cctgggcacg ctcgcgatcg tcggtggcgg cctcggccgg cggatgctcg agggtcgcgc
    12661 gcagcaggcc acggcggccg cgatcgagat cccgggggcg accgatcccg tcgccgcgcc
    12721 c
//
LOCUS       DBNHXO010000186         5921 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1041282, whole genome shotgun sequence.
ACCESSION   DBNHXO010000186 DBNHXO010000000
VERSION     DBNHXO010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5921)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1041282"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="AC162A_03980"
     CDS             <1..564
                     /locus_tag="AC162A_03980"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR5010029.1"
                     /translation="AVIAVAAPADPYRLTRQTFRLARLPIPAPVAWPLAWLTTRVYLR
                     PRGHTVDSVSATSAVRSIEGPVLLVHGSEDGVVPVGHLSRLADARRAACPNAVTETLL
                     VEGGRHSWLYEFPEYRAAIARFLTASLGGPFTPDEAAAVAAAVPAERLPDPERLTTLD
                     AEPGGLRSLTRLVRRREMAAPGPDAGG"
     gene            complement(567..1847)
                     /locus_tag="AC162A_03985"
     CDS             complement(567..1847)
                     /locus_tag="AC162A_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HIR5010030.1"
                     /translation="MTFPRLVLRDEVADLLDLPWEQPLGSWGDTGHRFRELPVGPSRH
                     LVRFLVVDGLVYALKELPEEVAGREYAVLRHLEDAKLPSVRAVGLAARPERGDAILVT
                     EYLRHSMQYRRLLMLLRPGSSHYRSRLLDAMALLLVDLHRAGVYWGDCSLANTLFRRD
                     GDRIQAYLVDAETSETFPTLSDGQRAYDLEILVENVAFGLADLAAYLGREEDMDEAIE
                     AAQSVRTGYEAVWRELNDQPELIPGDRQEIRARLRRLNDLGFSVDLEVDPVGPHGAVR
                     LKTSVTTRRYHASELERRTRIRALEGQARILLHDLAEYQAWLEFNERRSVPAEEAADR
                     WLREVYRPTQARIAGIVGRERDLVQAYCDVLEHKWLLSEQAGRDVGLDAAIESYLAEG
                     APAPERRDGDTAVGGDPALDALDILPDDGPALED"
     gene            complement(1882..2322)
                     /locus_tag="AC162A_03990"
     CDS             complement(1882..2322)
                     /locus_tag="AC162A_03990"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5010031.1"
                     /translation="MNDVAHITLRAAATGDAERLATLLTDEGYPAGPSDLAARIERFS
                     TPDAQVIVAEAGGEVAGFVAFHVVPRFETDERFIRIIAVVVDPLERERGVAHRLLADV
                     ERVARETGVSFVEVTAGHHRPEARQLFESLGYDASVTAYLRKRP"
     gene            complement(2355..3026)
                     /locus_tag="AC162A_03995"
     CDS             complement(2355..3026)
                     /locus_tag="AC162A_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010032.1"
                     /translation="MSLRQWAHLTWQASDPGEFAAELGRQLGIEIEPHPEAPGSFVLD
                     LGMATVEIVAWQRETPDDEPSPGGRLVFEPLPGGAPKPRIESLPDLLLAGVGWATVEL
                     DRAESELAEWLAGEPPDPDDDVTDAHLGARARVRDADGLPGDTMVLLEPTTEGRLAAS
                     LVRDGEGPCALYLWAPLGIDAWIAAAKGRGVVVSTRAAGPFGEAVLLPSRAPAGPHLV
                     VVERL"
     gene            complement(3034..3864)
                     /locus_tag="AC162A_04000"
     CDS             complement(3034..3864)
                     /locus_tag="AC162A_04000"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5010033.1"
                     /translation="MSRSVRIDDATTRRLERHETMAHAIPGRTVRDLGDALVLHDPRD
                     ADPFWNRMVSLRWPGEPAAFDHRLNEAITMFALDGRQPHIWPSPDHNAPSDLVGRLAA
                     VGFRDVGGGHVMILTDPSACPPLDDREIDPHVTVTGICGAADAGEDDIDDIAAVLAES
                     FGASPDRASELALDLRAIVGDSRVSLVLVRVDGQAAAVAKATGFDGFVYLSSIGTREA
                     YRGRGLAALATRHAVAISGGPAGAIVYLGVFTGNAPAIRLYERLGFASTGEAPDLLLG
                     "
     gene            3964..4365
                     /locus_tag="AC162A_04005"
     CDS             3964..4365
                     /locus_tag="AC162A_04005"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5010034.1"
                     /translation="MTRRLRLGALIATAALTLAACSGGGATVAPASPAPAASAPASVA
                     ASACSVGEGTGAAAEIKDFAFPTGLSVAAGQAIAWTNGDSANHTVTFDDGSCNTAVGS
                     GATVVVTYTVPGTYPFHCAIHTSMTGSLEVK"
     gene            complement(4366..5376)
                     /locus_tag="AC162A_04010"
     CDS             complement(4366..5376)
                     /locus_tag="AC162A_04010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5010035.1"
                     /translation="MRLDDRFDELVVSLGGFYRTWYVFAGLELGLLERLGHAGGEGLT
                     ADALAAQTGTEAELVRRWSWGASVHDLVDLDGDRIVLFEDVASVLLDADRPEYLGGQF
                     LHAAIGSLDFSALPDVLRTGQPLATRPDRYRMAIERLTVQDIAVFFQEALAALPQLVV
                     DLGDGAVIVDIHCGGGRWLIAMARRVPGTRLVGVEFEPDSVERARAHVAEAGLGDRIT
                     IEQGDLGAVGHENEVTLAYFQYALHQLADPASAIASAWHAIRPGGWLVALDWYLPTDP
                     GELRTRHAELIAGVQLDEMIQGTRLVTRSEALGWFANAGVPAPSLIDLPSGATVIVAQ
                     RA"
     gene            5468..>5921
                     /locus_tag="AC162A_04015"
     CDS             5468..>5921
                     /locus_tag="AC162A_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5010036.1"
                     /translation="MDDTELAESLARDLDASFEALVRAHADRCYAIALRVLGNSHDAE
                     EVAQDALVRAYRALTGYPAERIRDLRLRPWLATIVVNLCRNRTRRHTPPSTPLEPLVE
                     VGQEPVAARADDPARIADRAVDREHLAVLLATLPDRYRIPVVLRHVDDL"
BASE COUNT          828 a         2167 c         2146 g          780 t
ORIGIN      
        1 gccgtcatcg cggtcgccgc gccggccgac ccctaccggc tcacgcgcca gacgttccgg
       61 ctcgcccggc tgccgatccc cgcgcccgtg gcctggccgc tcgcctggct gaccacgcgg
      121 gtctacctcc ggcctcgcgg ccacaccgtg gactcggtga gcgcgacgag cgccgtgcgc
      181 tcgatcgagg ggcccgtcct gctcgtccac gggtcagagg acggcgtggt gccggtcggg
      241 cacctgtcgc gcctcgccga cgcccgccgg gcggcctgcc ccaacgcggt caccgagacg
      301 ttgctggtcg agggcggccg gcactcgtgg ctgtacgagt tccccgagta ccgcgcggcg
      361 atcgcgcgct tcctgaccgc gagcctcggc gggccgttca cgcccgacga agcggcggcg
      421 gtcgcggccg ccgtgcccgc cgagcggctc cccgaccccg agcgcctgac gacgctcgac
      481 gcggagcccg gcggcctgcg ctcgctgacg cggttggtgc gccgacgcga gatggcggcg
      541 cccgggccgg acgcgggagg ctgagctcag tcctcgaggg ccggcccgtc gtcgggcagg
      601 atgtcgagcg cgtccagggc cgggtcgccc ccgaccgccg tgtcgccgtc gcgccgctcc
      661 ggcgccgggg cgccctccgc gaggtacgac tcgatcgccg catcgaggcc aacgtcgcga
      721 ccggcctgct cggagagcag ccatttgtgc tccaggacgt cgcagtacgc ctggacgaga
      781 tctcgctcgc ggccgacgat cccggcgatg cgcgcctggg tggggcggta gacctcgcgc
      841 agccagcggt cggcggcctc ctcggccggc accgagcgcc gctcgttgaa ctcgagccag
      901 gcctggtact cggccaggtc gtggagcagg atccgcgcct ggccctcgag cgcccggatc
      961 cgcgtccgcc gctcgagctc gctcgcgtgg taccgacgcg tcgtcaccga cgtcttgagg
     1021 cgcaccgcgc catgtggccc gaccgggtcg acctcgaggt cgacggagaa cccgaggtcg
     1081 ttcagccgcc gcagcctcgc ccggatctcc tgccggtcgc cggggatcaa ctcgggctgg
     1141 tcattgagct ctcgccatac cgcctcgtag cccgtccgga ccgactgggc cgcctcgatg
     1201 gcctcgtcca tgtcctcctc gcgtccgagg taggcggcga ggtccgcgag accgaaggcg
     1261 acgttctcga cgaggatctc gaggtcgtac gctcgctgcc cgtccgagag ggtcgggaac
     1321 gtctcgctgg tctcggcgtc gacgaggtag gcctggatcc ggtcgccatc ccgacggaac
     1381 agtgtgttcg cgagcgagca gtcgccccag tagacgcccg cccggtgcag gtcgacgagg
     1441 agcagcgcca tcgcgtccag caggcggctg cggtagtggg agctgcccgg ccgcagcagc
     1501 atcaacaggc gccggtactg catcgaatgg cggagatact cggtcacgag gatggcgtcg
     1561 ccgcgttcgg gccgggcggc aaggcccacg gcgcggaccg atggcaactt ggcgtcctcg
     1621 aggtggcgga gcaccgcgta ctcgcgcccg gcgacctcct cgggcagctc cttgagcgca
     1681 tagacgaggc cgtccacgac gaggaagcgg acgaggtggc gcgacgggcc gacgggcagc
     1741 tcgcggaacc ggtgcccggt gtcgccccac gatccgagcg gctgctccca cgggaggtcg
     1801 aggaggtcgg cgacctcgtc gcggaggacc aggcgcggga aggtcatcgc gccgggcggg
     1861 cgtccggctg ccgggtcgcc gtcagggccg cttgcgcagg taggcggtga cgctcgcgtc
     1921 gtagcccagc gattcgaaca gctggcgcgc ctcgggccgg tgatgacccg ccgtcacctc
     1981 gacaaacgag acgcccgtct cgcgcgcgac gcgctcgacg tccgcgagca gacgatgggc
     2041 gacgccgcgc tcgcgctcga gcgggtcgac cacgacggcg atgatccgga tgaaccgctc
     2101 gtcggtctcg aaccgcggca ctacgtggaa ggcaacgaag ccggcgacct cgccgccggc
     2161 ctcggcgacg atcacctggg cgtcgggcgt cgagaagcgc tcgatcctcg ccgcgaggtc
     2221 gctcggcccg gccggatagc cctcgtccgt gagcagggtg gcgaggcgtt ccgcatcgcc
     2281 ggtggcggcg gcccgcagcg tgatgtgggc gacgtcgttc atgcggcgat ggtacccgtc
     2341 gccccgctcg cggactacag ccgctcgacg acgacgaggt ggggtcccgc cggcgcccgg
     2401 ctcggaagca ggacggcctc gccgaacggg ccggcggcgc gggtgctgac cacgacgccc
     2461 cgacccttcg ctgcggcgat ccacgcgtcg atcccgagcg gcgcccagag gtacagcgcg
     2521 cagggtcctt cgccgtcgcg caccagggac gcggccaggc gcccctcggt cgtcggttcg
     2581 aggaggacca tcgtgtcacc gggcaggccg tcggcgtccc ggacgcgcgc ccgcgcgcca
     2641 agatgcgcat cggtcacgtc atcgtcgggg tcgggcggct cccccgcgag ccactcggcg
     2701 agctcggact ccgcgcggtc aagctcgacg gtcgcccagc cgaccccggc cagcaacagg
     2761 tccggcaggg actcgatccg gggcttgggc gcgccgccgg gcagcggctc gaacacgagc
     2821 cgtccgcctg gcgacggttc gtcgtcgggc gtctcacgct gccaggcgac gatctcgacg
     2881 gtcgccatgc cgaggtcgag gacgaacgaa ccgggtgcct ccggatgtgg ttcgatctcg
     2941 atgcccagct gccgtccgag ctcggcggcg aactcgcccg ggtcggaagc ctgccaggtc
     3001 aggtgggccc actgcctcag cgacatcggc gcctcatccg agcagcaggt cgggcgcctc
     3061 gcccgttgac gcgaacccca ggcgctcgta gaggcggatc gccggcgcat tacccgtgaa
     3121 cacgcccagg tagacgatgg cgccggccgg gccgccgctg atggccacgg cgtggcgcgt
     3181 cgcgagggcc gcgagtcctc ggccacggta ggcctctcgc gtcccgatgg acgacaggta
     3241 cacgaagccg tcgaaccccg tcgccttggc gacggccgcc gcctggccgt ccacccgcac
     3301 gagcacgagc gacacgcgag aatcgccgac gattgcgcgc aggtcgaggg cgagctcgga
     3361 cgcccggtcg ggggacgccc caaacgactc cgcgaggaca gccgcgatgt cgtcgatgtc
     3421 gtcctcgccc gcgtcggccg cgccgcagat cccggtcacc gtcacgtggg gatcgatctc
     3481 ccgatcatcg agtggtgggc aggcggacgg gtccgtcagg atcatcacgt gaccgccccc
     3541 gacgtcgcgg aatccgaccg ctgcaaggcg gccgacgagg tccgatggcg cgttgtggtc
     3601 gggcgacggc cagatgtgtg gctggcgtcc gtccagggcg aacatcgtga tcgcctcgtt
     3661 gaggcgatgg tcgaaggccg ccggttcgcc tggccaacgg aggctgacca tgcgattcca
     3721 gaacggatcg gcatcgcgcg ggtcgtgcag gacgagtgca tcaccgagat cgcggaccgt
     3781 ccggcccggg atcgcatggg ccatcgtctc gtgccgttcc agccggcggg tggtggcgtc
     3841 gtcaatgcgg acggatcggc tcactcgaac acgatagccg cctggcccag cgcagcccgg
     3901 caggcgaaac cggctcgcat cctgcgggcg tatcgtcgtc atgacacgaa caggaggccc
     3961 agcatgaccc gacgactccg cctcggggcc ctcatcgcga ccgccgccct cacgctggcc
     4021 gcctgctcgg gcggtggcgc gaccgtggcg ccagcgtcgc ccgcccccgc ggcgtccgca
     4081 ccggcgtccg tcgccgcgag cgcgtgcagc gtgggcgagg gcacaggcgc ggccgcggag
     4141 atcaaggact tcgcgtttcc gaccggcctg agcgtggcgg ccggccaggc catcgcctgg
     4201 accaacggcg acagcgccaa ccacacggtg accttcgacg acgggtcgtg caacacggcg
     4261 gtgggcagcg gagccacggt cgtcgtgacc tacacggtgc ccggtacgta ccccttccac
     4321 tgcgcgatcc acacgagcat gacggggtcg ctcgaggtca agtagtcagg cgcgctgggc
     4381 gacgatcacg gtcgccccgg acggaaggtc gatcagcgag ggggcaggaa cgcccgcgtt
     4441 cgcgaaccag ccgagcgcct ccgagcgcgt caccagtcgc gtgccctgaa tcatctcgtc
     4501 gagttgcacg ccggcgatga gctctgcgtg gcgggtccgg agctcgccag ggtcggtcgg
     4561 gaggtaccag tcgagcgcga ccagccagcc gcccgggcgg atggcgtgcc aggccgaggc
     4621 gatcgcggac gccgggtcgg cgagctggtg gagcgcgtac tggaagtacg ccagggtgac
     4681 ctcgttctcg tggcccactg cgccgaggtc gccctgctcg atcgtgatcc ggtcgccgag
     4741 gccggcctcc gcgacgtggg cgcgggcccg ctccaccgag tcgggctcaa actcgacgcc
     4801 gaccagccgc gtccccggaa cccgcctggc catcgcgatg agccagcggc cgccgccgca
     4861 gtggatgtcg acgatcaccg cgccgtcccc gaggtcgacc acgagctgcg gcagcgcggc
     4921 gagggcctcc tggaagaaga ccgcgatgtc ctgcacggtc aggcgctcga tcgccatccg
     4981 gtagcggtcg ggacgcgtcg ccaacggctg cccggtgcgg agcacgtcag gcagcgcgga
     5041 gaagtcgagg ctcccgatgg ccgcgtgcag gaactgcccg cccaggtact ccggccggtc
     5101 ggcgtcgagc agcacggacg cgacatcctc gaacagcacg atccgatcgc catcgaggtc
     5161 gaccaggtcg tggaccgagg ctccccatga ccagcggcgg acgagctccg cctcggtccc
     5221 ggtctgggcg gccagcgcgt cggccgtgag gccctccccg cccgcatggc ccagccgctc
     5281 cagcagtccc agctcgaggc ccgcgaacac gtaccacgtg cgatagaacc cgccgaggct
     5341 gaccacgagc tcgtcgaagc ggtcgtcgag gcgcatggcc ggattgtgac atgctgcctc
     5401 gggcggtccg ggcacgattg agtgacgccc tgcaaccgtc cgggcgtcgg gcgagttctg
     5461 gatggtgatg gacgacaccg agctggccga gtcgctcgcg cgggacctcg acgcctcgtt
     5521 cgaggcgctc gttcgggcgc atgccgatcg ctgctacgcg atcgcgctgc gcgtcctggg
     5581 gaactcgcac gacgccgagg aggtcgccca ggacgcgctc gtgcgggcct accgcgcgct
     5641 gaccggctat cccgccgagc ggatccggga cctccgcctc cggccgtggc tggcgacgat
     5701 cgtcgtcaat ctgtgccgca accggacccg gcggcacacg cctccctcga cgccgctcga
     5761 acccctggtc gaggtgggcc aggagcccgt ggcggcacgc gccgacgatc cggccaggat
     5821 cgccgaccgc gccgtcgatc gagagcacct cgccgtcctg ctggcgaccc ttcccgaccg
     5881 ctatcgcatc ccggtcgtcc tgcgccacgt cgacgacctc t
//
LOCUS       DBNHXO010000187         1855 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3421361, whole genome shotgun sequence.
ACCESSION   DBNHXO010000187 DBNHXO010000000
VERSION     DBNHXO010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3421361"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1229
                     /locus_tag="AC162A_04020"
     CDS             120..1229
                     /locus_tag="AC162A_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR5010037.1"
                     /translation="MTKIVSRRADRVTVRFRRPFPVAAGMWTAREAWILRLTDADGRV
                     GLGEAVVEPDDGEVAGTVLESLVREAVSMAAGGVLPTGDELEGHGRPGRALRAGLDGA
                     RFDLDGVPLPALRADGEGIGVNAVLPLLGPRPSMEAALQAVAAGFTTLKLKAGAERET
                     EVLVDRVRAVRDAIGPDVRLRLDANGAWDAATAEERLEAVTRFDLEYVEQPLAGDDPA
                     ALADLRRRVRVPIAADETVGSLRAARELLEADAVDVLVVKPARVGGLVVGTAIAELAV
                     EHRVPVVVSTLFETGVGIAAALAMAAGMPVVNEPPDHGLATAGVLEHDLLREELVVDD
                     GRMWLPDRPGPGGLGIEIDDDALARYRAEAVEAVG"
     gene            1226..>1855
                     /locus_tag="AC162A_04025"
     CDS             1226..>1855
                     /locus_tag="AC162A_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5010038.1"
                     /translation="MTASPDDEAVALLDGGSVVSWAELDRRCAAAAGGMVVVDGTASI
                     DAVVTILGVVRAGGVAAIVPPGLTAAERATAVGVFDAAAHDGPGVVVLTSGTTARPKG
                     VVLSGAALAASADAWLAALPPATGWLLALGLGHVAGLGVLWRAIAGRVPMRIVSGAEP
                     AAILEALSSEPPLSHVSLVPAQLARLLDECGDAKPPPTLRAVLLGGGTIP"
BASE COUNT          199 a          627 c          770 g          259 t
ORIGIN      
        1 ggcgagccga gggcgttgaa cccggtgctc aaggggaccg cccggctcgc gttcgccttc
       61 tcggtcctgt tcgcggtcgg actcgcgctg gccggcgccg cctacggagg gccgtcggcg
      121 tgacgaagat cgtttcgcgt cgggccgacc gcgtcaccgt ccggttccgc cggccgttcc
      181 cggtggcggc ggggatgtgg accgcgcgtg aggcatggat cctgcggctg accgacgccg
      241 acggccgggt cggcctgggg gaagccgtgg tcgagcccga cgatggcgag gtcgcgggga
      301 cggtgctcga gtcgttggtg cgcgaggcgg tctcgatggc ggcgggcggc gtcctgccga
      361 ccggcgacga gctggaggga cacggacggc cgggacgggc gctgcgggcg gggctcgatg
      421 gcgcccggtt cgacctcgac ggggtcccgc tgccggccct ccgcgccgat ggcgagggca
      481 ttggcgtgaa cgccgtgctg ccgctgctcg gcccccggcc ctcgatggag gccgcgctgc
      541 aggcggtcgc agcggggttc acgaccctca agctgaaggc cggtgccgag cgggagacgg
      601 aggtgctcgt cgatcgggtg cgggcggtcc gcgacgcgat cgggccggac gtgcggctgc
      661 ggctcgacgc gaacggggcg tgggatgcgg cgaccgccga ggagcggctc gaggccgtga
      721 cccggttcga cctcgagtac gtggagcagc cgctggccgg cgacgacccg gccgcactgg
      781 cggacctccg gcgccgcgtc cgggtgccga tcgccgccga cgagacggtc ggctcgctca
      841 gggcggcgcg ggagctcctc gaggccgacg ccgtcgacgt gctggtcgtg aagccggcgc
      901 gggtcggcgg actcgtggtc ggcacggcga tcgccgagct ggcggtcgag catcgggtgc
      961 cggtcgtggt cagcaccctg ttcgagacgg gcgtgggcat tgccgcggcg ctggccatgg
     1021 cggcggggat gccggtcgtg aacgaaccgc cggaccatgg cctggcgacg gccggcgtgc
     1081 tcgaacacga cctgctgcgc gaggagctcg tggtcgacga tgggcggatg tggctgcccg
     1141 accgtcccgg gcctggcggg ctggggatcg agattgacga tgacgcactg gcgcgctatc
     1201 gcgccgaagc ggtggaggcg gtgggatgac cgcgtcgccg gacgacgagg cggtcgccct
     1261 gctcgacggc ggcagcgtgg tgtcgtgggc cgagctcgac cgccgctgcg ctgccgccgc
     1321 gggcggcatg gtcgtcgtcg atggcacggc atccatcgac gcggtcgtca ccatccttgg
     1381 cgtggtccgc gccggtgggg tggccgccat cgtgccgccg ggcctcacgg ccgccgagcg
     1441 ggcgacggcc gtgggcgtgt tcgatgccgc cgcgcacgac ggccccgggg tcgtcgtgct
     1501 cacctccggc acgaccgcgc ggccaaaggg cgtcgtcctg tccggtgccg ccctggcggc
     1561 gagcgccgac gcgtggctcg ctgcactgcc gccggcgacg ggatggctcc tcgcgctcgg
     1621 cctcgggcat gtcgccgggc tcggcgtgct gtggcgcgcg atcgccgggc gtgtcccgat
     1681 gcggatcgtg tccggtgccg aaccggcggc gatcctcgaa gcgctgagca gcgagccgcc
     1741 gctgagccac gtgtcgctcg tgccggcgca gctcgcgcga ctgctcgacg aatgcgggga
     1801 tgctaagccg ccgcccacgc ttcgggccgt cctgctgggt ggagggacga ttcct
//
LOCUS       DBNHXO010000188         2719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3819464, whole genome shotgun sequence.
ACCESSION   DBNHXO010000188 DBNHXO010000000
VERSION     DBNHXO010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3819464"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="AC162A_04030"
     CDS             <1..232
                     /locus_tag="AC162A_04030"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5010039.1"
                     /translation="TVGAAWYCNREDDLGSIETGKLADLVVLDRDYFRVSDADVRNIR
                     PILTVVDGKVVHDTGALGRHKHDDDDDDHRRR"
     gene            210..698
                     /locus_tag="AC162A_04035"
     CDS             210..698
                     /locus_tag="AC162A_04035"
                     /inference="COORDINATES: protein motif:HMM:NF023164.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HIR5010040.1"
                     /translation="MTITGAVSATFEGVRLMNRRLAAGAAALLLGLHAVVSFADDKRP
                     LVSWDALGQVTGVMDKGRYVPRFPKEIAALDKREVRLEGFMMPLDMGVQQKRFLLTSV
                     APTCGFCMPSGAEQIVEIQARAPVKYVLDPVVLSGRFVLVHDDTGGLLYRLTDAIAVE
                     KK"
     gene            745..2538
                     /locus_tag="AC162A_04040"
     CDS             745..2538
                     /locus_tag="AC162A_04040"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR5010041.1"
                     /translation="MTKDRRPGRGFSRREFIQAAAAAAGGTMFTTPALALNPRWKKSR
                     PKHPDPDLILVDGRIHTMNARNRVVSSVAIKDGRFVEVGNVGRHHYGRETKVIDLNGR
                     TVVPGIIDNHNHLVLMGNRPGYHTPLENAYSIPDVQAIYKARAAGIPPGAWITTIGGF
                     HSNHIYGTPGDPTSGRLPTLAELDAAVPGNPVFIEIGFTGPSVTNSAGKSILTANGVT
                     VGATGDAGSIAAGTQSSTALLYLRQTLLNPEQRRRGVLDAMNYAASVGVTTHLDQGAF
                     QATNTPSDGAAHEDNYTMQLPFLDVYDRGEGIVRLRINFLHMEANPETPELRERLRNA
                     YPFLGDDMVKTGGIGEFIAQGTNPATAPQFLAAARRVAAANWRAEVHSLGRRNTPTSP
                     PADFEFEIQAFETVNGEFPGVVRDRRWVIAHVPGITQEWIDRFQAIGGNLSLTGWQYL
                     AGNPTASTVAPYAGPPFRMIVDSSRRADGIHVGMSSDGMQIAPMNPWIHMYYATTGLN
                     ARNVLINPNQQISRDEALSLYTRENGWFLREEDQLGSIEEGRLADLVVLNRDYFSVAA
                     EDLKKIRSVLTVVDGDVVHDDLRGGHHHHRG"
BASE COUNT          471 a          954 c          923 g          371 t
ORIGIN      
        1 caccgtcggc gcggcctggt actgcaaccg cgaggacgac ctcggctcga tcgagaccgg
       61 caagctggcc gacctcgtcg tactcgaccg cgactacttc agggtcagcg acgcggacgt
      121 gcgcaacatc cgtccgatcc tcacggtggt cgacggcaag gtggtccacg acaccggcgc
      181 cctcggtcga cacaagcacg acgacgacga tgacgatcac aggcgccgtt agcgctacgt
      241 tcgagggcgt aaggctgatg aacaggcgcc tggccgccgg tgcagcagcg ttgctgctgg
      301 gtctgcatgc cgtcgtctcg ttcgcggacg acaagcgccc gctggtttcc tgggacgcgc
      361 tcggccaggt caccggcgtc atggacaagg gccgctacgt cccgcgattc ccgaaggaga
      421 tcgccgccct cgacaagcgg gaagtgcggc tcgagggctt catgatgccg ctcgacatgg
      481 gtgtgcagca gaagcgcttc ctgctgacgt cggtggcacc gacctgcggg ttctgcatgc
      541 cgagcggcgc cgagcagatc gtcgagatcc aggcgcgggc cccggtgaag tacgtcctcg
      601 atcccgtcgt gctgtccggg cggttcgtgc tggtgcacga cgacaccggg ggccttctgt
      661 accggctcac cgacgcaatc gcggtcgaaa agaaatgacg ccggccgcga tccgcggcac
      721 tcatcgcaaa ggaggaacgc tgacatgacg aaggacaggc gcccagggcg cggcttctcg
      781 cgccgcgagt tcatccaggc agcagccgcc gcggccggcg ggacgatgtt cacgacgccc
      841 gcgctcgcgt tgaatccgcg atggaagaag tcgcgaccca agcacccgga cccggacctc
      901 atcctcgtcg acggccggat ccacacgatg aacgcgcgga acagagtcgt gtcgtcggtg
      961 gcgatcaagg acggccgctt cgtcgaggtc ggcaacgtcg ggcggcacca ctacgggcgc
     1021 gagaccaaag tcatcgacct caacggacgg acggtcgtcc cgggcatcat cgacaaccac
     1081 aaccacctgg tgctgatggg caaccggccc gggtatcaca cgccgctcga gaacgcgtac
     1141 tcgatcccgg acgtccaggc gatctacaag gcgcgcgcgg ccggtattcc gccgggcgcg
     1201 tggatcacca ccatcggcgg gttccactcg aatcacatct atggcacgcc cggcgacccg
     1261 acgagcggcc gcctgccgac gctcgcggag ctggacgccg cggtgcccgg caacccggtg
     1321 ttcatcgaga tcgggttcac cggaccgtcg gtcaccaaca gcgcgggcaa gtcgatcctc
     1381 acggcgaacg gcgtcaccgt gggtgccacc ggcgacgccg gctcgatcgc cgcggggacc
     1441 cagtcgtcga ccgccctgct ctatctgcgc cagacgctgc tcaacccgga gcagcgccgg
     1501 cgcggcgtgc tcgacgccat gaactacgca gcgagcgtcg gggtgaccac gcacctcgac
     1561 cagggcgcgt tccaggcgac caacaccccg agcgacggcg cggcgcacga ggacaactac
     1621 acgatgcagc tgccgttcct cgacgtctac gaccgcggcg aggggatcgt ccggctgcgc
     1681 atcaacttcc tgcacatgga ggccaacccg gagacgcccg agctgcggga gcggctgagg
     1741 aacgcgtatc ccttcctcgg cgacgacatg gtgaagaccg gcggcatcgg cgagttcatc
     1801 gcccagggca cgaatccggc gacggcgccg cagttcctcg ccgcagcgcg acgggtggcc
     1861 gccgccaact ggcgggcgga ggttcattcc ctcggccgcc gcaacacgcc tacgagcccg
     1921 ccggccgact tcgagttcga gatccaggcg ttcgagaccg tcaacggcga gttcccgggc
     1981 gtcgtccgcg accggcgctg ggtgatcgcc cacgtccccg gcatcacgca ggagtggatc
     2041 gaccgcttcc aggcgatcgg cggcaacctc tcgctcaccg gatggcagta cctcgccggc
     2101 aacccgacgg ccagcacggt cgcgccgtac gccggaccgc cgttcaggat gatcgtcgac
     2161 agcagccggc gggccgacgg catccacgtc gggatgagct ccgacggcat gcagatcgcg
     2221 ccgatgaatc cgtggatcca catgtactac gcgaccaccg ggctcaacgc gcgcaacgtc
     2281 ctcatcaacc cgaaccagca gatctcgcgc gacgaggcgc tgagcctcta cacgcgggag
     2341 aacggctggt tcctgcgcga ggaagaccag ctcggcagca tcgaggaagg ccggctcgcg
     2401 gacctcgtgg tcctcaaccg ggactacttc tccgtggcgg ccgaggatct gaagaagatc
     2461 cggtcggtgc tgacggtggt cgacggcgac gtggtccacg acgatctccg cgggggacat
     2521 caccaccatc gcggatgaac gccggcgcgt cgcggtgcgg ggccgggccg gacggcgtcc
     2581 ggcccctcct ctatggcggg cgccgcgtcg tggtcgatgg ccacgcgatc tgcagcgaat
     2641 gcggtgcggc ggtcgagctc gccgcgccgg atcggtggcg ccacgcgccg cgcgggcgcg
     2701 cgcggcggcc accgcgaat
//
LOCUS       DBNHXO010000189         2359 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5407398, whole genome shotgun sequence.
ACCESSION   DBNHXO010000189 DBNHXO010000000
VERSION     DBNHXO010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5407398"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="AC162A_04045"
     CDS             <1..344
                     /locus_tag="AC162A_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5010042.1"
                     /translation="YARTYKLANRIFFGLKVRDVDCACKLFRREALEGVRVESGGAFF
                     SAELLIKIQAAGRSVAQVGVPHYPRTAGSPTGAKPSVIGRAVKDFWSLRLRMWANRQR
                     AGRRGRPILGE"
     gene            complement(361..963)
                     /locus_tag="AC162A_04050"
     CDS             complement(361..963)
                     /locus_tag="AC162A_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HIR5010043.1"
                     /translation="MDGDRGSEIEMIVESVRVHMPTGRHVLVLREVDATRLLMVWVGP
                     WEASAIAMKLQGLTPERPLTHDLFATVLTTLGVTIERIVITDLADETYHARLELSRRG
                     DRHEIDARPSDAIALAVRLGVPVFAAADVLDRAATVPDEDDDEDEEGEEAGATATRRR
                     KPADPESTDEVLDQTRLAIFREFVNSLDPDADQRGGASSS"
     gene            complement(984..1688)
                     /locus_tag="AC162A_04055"
     CDS             complement(984..1688)
                     /locus_tag="AC162A_04055"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR5010044.1"
                     /translation="MIPLRDANPTRRTPVVTLTLIALCCGVFAWELVVLAQGGDQGLG
                     DFLTSYGAVPADVAEAARTGHWLSLPVLGVLTSIFLHADWWHLLGNMLYLWIFGNNVE
                     DWLGRPMFLVFYLVGGLAAVAGQVAVDMSSDVPMIGASGAISAVLGAYLVLFPGARIQ
                     SLVFLGLFYQLIAVPAVLVLGFWFALNLLDAIGWIGAGGETGVAFFAHVTGFLAGVLM
                     ALPFRLRRQPTPALAT"
     gene            complement(1705..>2359)
                     /locus_tag="AC162A_04060"
     CDS             complement(1705..>2359)
                     /locus_tag="AC162A_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010045.1"
                     /translation="IKDIATFVANFNAALYNLYVVDPSEQNVMVFATASDGNRFPRGG
                     NARLPTNRPIDGITDLLIDGDIYAAENGQVGRLIPATTWKADPPADTQLRPTSDYTML
                     AAPVLANGDPNKRIGALYAFDAINHRIVAFQKSDGRYLGQYQVSGDADEWSDLQGLFV
                     LPAADPEAPPTLWWISSKGLSSALLEEAPEETPVASPSAEPTKAPAKTPKPTKKPKN"
BASE COUNT          349 a          810 c          863 g          337 t
ORIGIN      
        1 tctacgcccg cacctacaag ctcgccaacc gcatcttctt cggcctcaag gtcagggacg
       61 tcgactgcgc ctgcaagctg ttccgccgcg aagcgctgga gggcgtccgg gtcgaatcgg
      121 gcggcgcgtt cttctccgcg gagctgctga tcaagatcca ggcggccggc cgatccgtcg
      181 cccaggtcgg cgtcccgcac tacccacgca cggcggggtc gccgacgggc gccaagccgt
      241 cggtcatcgg gcgcgccgtg aaggacttct ggagcctgcg cctgcggatg tgggcgaacc
      301 ggcagcgcgc cgggcgccgc ggccggccga tcctgggcga gtagtccgcg agagcggcgc
      361 ctagctcgag gaggcgccgc cgcgctgatc ggcgtcggga tcgagcgagt tcacgaactc
      421 acggaagatc gcgaggcggg tctggtccag gacctcgtcc gtcgactccg ggtcggcggg
      481 cttccggcgt cgcgtcgcgg tcgcgccggc ctcctcgccc tcttcgtcct cgtcgtcgtc
      541 ctcgtcgggc accgtggccg cgcggtcgag gacgtccgcg gccgcgaaca ccgggacgcc
      601 gagccgcacg gcgagcgcga tcgcgtccga cggccgggcg tcgatctcgt gccggtcgcc
      661 gcgacggctg agctcgagcc gggcgtggta ggtctcgtcg gcgaggtcgg tgatgacgat
      721 ccgctcgatg gtcacgccca gcgtggtcag gacggtcgcg aacaggtcgt gggtcagcgg
      781 gcgctccggc gtgagcccct ggagcttcat cgcgatcgcg ctggcctccc aggggcccac
      841 ccagaccatc agcaggcggg tggcgtcgac ctcgcgcagg acgaggacgt ggcggccggt
      901 cggcatgtgc acccgcacgc tctcgacgat catctcgatc tcggagccgc ggtcgccgtc
      961 catgcgccga ttatcccacg cggtcaggtg gcgagggcgg gcgtgggctg gcggcggagc
     1021 cggaacggca gggccatcag cacgcccgcg aggaagccgg tcacgtgggc gaagaacgcc
     1081 acgcccgtct cgccgccggc accgatccag ccgatcgcgt cgagcaggtt gagcgcgaac
     1141 cagaacccga gcacgagcac ggccgggacc gcgatcagct ggtagaacag cccgaggaac
     1201 acgagcgact ggatccgcgc cccggggaac aggacgaggt aggcgcccag gaccgccgag
     1261 atcgcgcccg acgccccgat catcgggacg tccgacgaca tgtcgacagc gacctggccc
     1321 gcgaccgcgg cgaggccgcc gaccaggtag aacacgagga acatcggccg cccgagccag
     1381 tcctcgacgt tgttgccgaa gatccacagg tacagcatgt tgccgagcag gtgccaccaa
     1441 tcggcgtgca ggaagatgct ggtcaggacg cccaggaccg gcagcgacag ccagtgcccc
     1501 gtgcgggccg cctccgccac gtcggcaggc acggccccgt agctcgtcag gaaatcaccc
     1561 agcccctggt caccgccctg ggccagcacc accagctccc aggcgaacac gccgcaacac
     1621 aaggcgatga gcgtgagggt gacgacgggg gtgcggcggg tcgggttggc gtcacgaagc
     1681 gggatcaccg tcgaccgccg ccggtcagtt cttcggcttc ttcgtgggct tgggcgtctt
     1741 ggccggcgcc ttggtcggct ccgcgctggg cgacgcgacc ggcgtctcct cgggggcctc
     1801 ctcgagcagc gcgctgctca gacccttgct cgagatccac cacagggtcg ggggggcctc
     1861 cgggtccgcc gccggcagga cgaagaggcc ctggaggtcg gaccactcgt cggcgtcgcc
     1921 ggagacctgg tactggccga ggtacctgcc gtcggacttc tggaacgcga cgatccggtg
     1981 gttgatggca tcgaacgcgt acagggcgcc gatgcgcttg ttcggatcgc cgttcgcgag
     2041 aacgggcgcc gcgagcatcg tgtagtcgct cgtcgggcgg agctgcgtgt ccgccggcgg
     2101 gtccgccttc caggtcgtgg cggggatgag gcgcccgacc tggccgttct cggcggcgta
     2161 gatgtcgccg tcgatgagca ggtcggtgat gccgtcgatc ggccggttgg tcggcaggcg
     2221 ggcgttcccg ccacgcggaa atcggttccc atcgctcgcc gtcgcgaaca ccatgacgtt
     2281 ctgctccgac gggtcgacga cgtacaggtt gtagagcgcc gcgttgaagt tggcgacgaa
     2341 cgtcgcgatg tccttgatg
//
LOCUS       DBNHXO010000190         1761 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1043525, whole genome shotgun sequence.
ACCESSION   DBNHXO010000190 DBNHXO010000000
VERSION     DBNHXO010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1043525"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..566)
                     /locus_tag="AC162A_04065"
     CDS             complement(180..566)
                     /locus_tag="AC162A_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010046.1"
                     /translation="MLNLRLVTAAALVVTAMAIGASPVAAKSSDVVMRGNCSGSADWK
                     LKAGKDNGQIEVEFEVDSNHAGQVWRVKILDNDVLRHRARYATHAPSGSFSVDRRIAN
                     MAGPDYIVARARNLASGQTCVGKLTL"
     gene            713..>1761
                     /locus_tag="AC162A_04070"
     CDS             713..>1761
                     /locus_tag="AC162A_04070"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR5010047.1"
                     /translation="MHDQGPGRLSRRALDPAFELAAAQVAAGTVPFVILGVADRVGTI
                     RLESFGMTVGPRIGVDALCLLASITKPIVATAVMHEVEAGRLDLDQPLVGLLPELATA
                     GAGSFTAWHVLTHTSGIGDVYVEGLLLEGGDRAELLRRAIALPQRSVPGSAFDYATAP
                     FDILAEGIARRVGRPFEAVLREDVLDPLGMTETVFAPGPDAAPRMAPVAVDLPALAGI
                     PGSALIAGFTGLRLAGGGLWSTAGDVLRFGRAMLRGGELDGRRVLSPAFVDLMTREVT
                     VGGLGAADDVQRAEHYAIGWGKPGVSSPASASSFGHGGGSGTRLWVDPAHDLVFLYLT
                     GSWAMSHEPINAVQHA"
BASE COUNT          221 a          618 c          646 g          276 t
ORIGIN      
        1 gccgccccca tctcgcccca cgcgttggcc agccggatcc ggatctcctc ggcgatcggg
       61 gagccggacc gcggggtcgc gtcgagggcg cgggcggcgt cggtcacccc ccgatcctac
      121 tccgatcgcg aaacctgcgc ccggtcgtta gccgattttt gagggccagg ccctaggcgt
      181 cagagggtca gcttgccgac gcaggtctgg ccgctggcca ggttgcgggc ccgggcgacg
      241 atgtagtcgg gtccggccat gttcgcgatc ctgcggtcga cgctgaacga gcccgacggc
      301 gcgtgggtcg cgtagcgcgc gcggtggcgg agcacgtcgt tgtcgaggat cttcacccgc
      361 cacacctggc cggcgtggtt cgagtcgacc tcgaactcga cctcgatctg cccgttgtcc
      421 ttgcccgcct tgagcttcca gtcggcagag ccggagcagt tgccccgcat gacgacgtcc
      481 gacgactttg cggcgacggg cgacgcgcca atggccatgg cggtgaccac gagggcggcg
      541 gcagtgacga gccgaaggtt gagcatcgtg tcctccaagg gggcatgtca ggtcgggtgg
      601 gcgagtgcga cgcctcactc ctcttcgagg caccaacccc gcagaaggtt tcatgggcgg
      661 gggcgacgct cctacccacg ttgttcgtca cgcggaccgc tatggttccc cgatgcacga
      721 ccaaggaccc ggccgcctgt cacgtcgcgc cctcgacccg gcgttcgagc ttgccgccgc
      781 gcaggtcgcc gcgggcacgg tcccgttcgt gatcctcggc gtggccgacc gtgtcgggac
      841 gatccgcctc gagtcgttcg ggatgacggt cgggccgcga atcggcgtcg acgcgctgtg
      901 cctgctcgcc tcgatcacga agcccatcgt cgcgacagct gtcatgcacg aggtcgaggc
      961 gggccgcctt gacctcgatc agccgctggt cgggctgctg ccggagctcg cgacggcggg
     1021 cgccggatcc ttcacggcct ggcacgtgct cacgcacacg agcggcatcg gcgacgtcta
     1081 cgtcgaaggg ctgctgctcg aaggcggcga ccgggcagag ctcctccggc gcgccatcgc
     1141 gttgccgcag cgcagcgtcc cgggatcggc gttcgactat gccacggccc ccttcgacat
     1201 cctcgcggag ggcatcgccc ggcgggtcgg ccgcccgttc gaggccgtgc tccgggagga
     1261 cgtgctcgac ccgctcggga tgaccgagac cgtgttcgcg cccgggcccg acgccgcgcc
     1321 gcgcatggcg cccgtggcgg tcgatctgcc cgccctggcg gggatccctg ggtccgcgct
     1381 gatcgccggc ttcacgggcc tgcggctggc cggcggtggc ctttggagca cggcgggcga
     1441 cgtgctcagg ttcgggcgcg cgatgctccg tggcggcgag ctggatggcc ggcgggtgct
     1501 gtcgcccgca ttcgtcgacc tgatgacgcg cgaggtgacg gtcggcgggc tcggcgcggc
     1561 ggacgacgtc cagcgggccg agcactacgc catcggctgg gggaagcccg gggtctcgag
     1621 cccggcatcg gcatcgtcgt tcgggcacgg cggcggaagc gggacgcgcc tgtgggtcga
     1681 cccggcccac gatctcgtgt tcctgtacct gaccggatcc tgggcgatga gccacgagcc
     1741 gatcaacgcc gtgcagcacg c
//
LOCUS       DBNHXO010000191         2986 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4812111, whole genome shotgun sequence.
ACCESSION   DBNHXO010000191 DBNHXO010000000
VERSION     DBNHXO010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4812111"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="AC162A_04075"
     CDS             <1..540
                     /locus_tag="AC162A_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HIR5010048.1"
                     /translation="LLAAMLVYFFSSLAIRAVGTTAQSIIVEVRRQFREMPGIMDYTQ
                     RPDYARVVDITTRAALREMVAPGLVAVATPIIIGVVLGFEAVAGMLMVGTISGVLLAT
                     VLNNGGGAWDNAKKYIESGHLKDDQGNVLGKGSEAHAAAVVGDTVGDPFKDTAGPSLH
                     VLVKLLATISLVLAPLFIR"
     gene            582..1406
                     /gene="uppP"
                     /locus_tag="AC162A_04080"
     CDS             582..1406
                     /gene="uppP"
                     /locus_tag="AC162A_04080"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HIR5010049.1"
                     /translation="MEQIFQAVVMGVVQGLTEFLPISSSGHLILVPYLFGWDDPFLES
                     LAFSVVLHAGTLGALLVYFREDWLRLVPAGFAALRDRSFEGDPYRRLAWLIAIATVPA
                     IIAALLFNDLIEDAVRQAGLVAVMLLIGAAILWLADRLGPRTHRLGDLTFRGSLGIGI
                     AQALALIPGVSRSGISISAALFAGLDREGAARFSFLMATPITALAVVYEGFKLIRGDV
                     TGVEVAPMIAGVVAAFVAGILAISVLLRFVRTHSYGIFVAYRVVLAAIVIVVFLVR"
     gene            1438..1902
                     /gene="argR"
                     /locus_tag="AC162A_04085"
     CDS             1438..1902
                     /gene="argR"
                     /locus_tag="AC162A_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIR5010050.1"
                     /translation="MKQLRQRAIRDLVEQRPIRTQQELAAALRERGFRTTQATISRDV
                     AELGLIKVRRAGTQAYAIPPRLREAEASGEDRIRTLLHDMPVEIREAGTTLVLRTLPG
                     SAHPLAAALDRARWPEVVGSIAGDDTVFVAFADRTSLRRVRRRLEEIGQLPD"
     gene            complement(1899..2888)
                     /locus_tag="AC162A_04090"
     CDS             complement(1899..2888)
                     /locus_tag="AC162A_04090"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HIR5010051.1"
                     /translation="MTAAVDPPAIRRVVGVALNAAIDKTVSVDRLVAGAIHRPVIRSV
                     VAGGKAANVVRAARHMGLDATMVAVLGGHAGAWYRAQLDERSIALHEVAATDETRTCL
                     SVLDETTGELTEFYEAGIRLRNEDWPRVEDALRAAIGDDGSDVVVVLAGSLPPGTPVD
                     AYGRLAVIAAELGARAVVDSDGDSLAAALVTRPWLVKVNATEAETVTGTSARTRDRAA
                     TAAAELRSRGAANAIVTRGVHGAAVATADGSWALGNLPAAHRGPYSVGSGDAFLAGFL
                     AGSSRGLGPADALRVAGATGAANARTPGQGELDPADVDRTRLVCEVVALPART"
BASE COUNT          367 a         1106 c         1055 g          458 t
ORIGIN      
        1 ctgctggcgg cgatgctcgt gtacttcttc agctcgctcg cgatccgcgc ggtgggcacg
       61 acggcccagt cgatcatcgt cgaggtccgg cgccagttcc gcgagatgcc cgggatcatg
      121 gattacaccc agcgcccgga ctacgcccgc gtcgtggaca tcacgacccg tgccgccctg
      181 cgcgagatgg tcgcgcccgg tctcgtcgcc gtcgccacgc cgatcatcat cggggtcgtg
      241 ctcggcttcg aggcggtcgc cggcatgctg atggtcggga cgatctcggg cgtcctgctc
      301 gctaccgtcc tcaacaacgg cggcggcgcg tgggacaacg ccaagaagta catcgagtcg
      361 ggccacctca aggacgacca gggcaacgtg ctcggcaagg gctcggaagc ccacgcggcg
      421 gccgtcgtcg gcgacacggt cggcgatccg ttcaaggaca cggccggccc gtcgcttcac
      481 gtcctggtca agctgctcgc cacgatctcg ctggtgctgg cgcccctgtt catccgctag
      541 ggtccgttca ccgaggtcga ccggactagc ctgaggtcgt cgtggagcag atctttcagg
      601 ccgtcgttat gggcgtcgtc caggggctca ccgagttcct gcccatttcg tcgtccggtc
      661 acctgatcct ggttccgtac ctgttcggct gggacgaccc gttcctcgag tcgctcgcgt
      721 tctcggtcgt gctccacgcc gggacgctgg gtgcgctgct cgtgtacttc cgcgaggact
      781 ggctccggct cgtgccggcc gggttcgccg ccctccgcga ccgctcgttc gagggcgacc
      841 cgtaccggcg gctcgcgtgg ctgatcgcga tcgcgaccgt gcccgcgatc atcgccgcgc
      901 tgctgttcaa cgacctgatc gaggacgccg tccgccaggc gggcctcgtc gcggtgatgc
      961 tcctgatcgg ggcggcgatc ctgtggcttg cggaccggct ggggccgcgg acgcaccggc
     1021 ttggcgacct gacgttccgg ggctcgctcg gcatcggcat cgcgcaggcc ctggcgctca
     1081 tccccggcgt gagccgatcg gggatctcga tctcggccgc cctgttcgcc ggcctggacc
     1141 gggagggcgc ggcgcgcttc agcttcctga tggcaacgcc gatcacggcg ctcgccgtcg
     1201 tgtacgaggg gttcaagctg atccgagggg acgtgacggg ggtcgaggtc gcgccgatga
     1261 tcgccggggt cgtggccgca ttcgtcgccg gcatcctcgc gatttccgtg ctgctgcggt
     1321 tcgtccggac gcactcgtat gggatcttcg tcgcgtaccg cgtggtgctg gccgccatcg
     1381 tcatcgtggt cttcctggtc cgctaggacg acgctgaggc cgatccccat ggaagtcatg
     1441 aagcagctcc gccagcgagc gatccgcgac ctggtcgagc agcgcccgat ccggacccag
     1501 caggagctgg ccgccgcgct gcgcgagcgt ggcttccgga cgacgcaggc aacgatcagc
     1561 cgcgacgtcg cggagctcgg cctgatcaag gtccgccgcg ccggcacgca ggcctacgcg
     1621 atcccgccgc gcctgcgcga ggccgaggcg agcggcgagg accgcatccg gacgctcctg
     1681 cacgacatgc cggtcgagat ccgggaggcc ggcaccacgc tggtgctgcg cacgctgcca
     1741 ggctcggcgc acccgctggc ggcggcgctc gatcgcgccc gatggcccga ggtcgtgggc
     1801 tcgatcgccg gcgacgacac ggtgttcgtg gcgttcgccg accgcacgtc gctgcgccgc
     1861 gtccggcgcc gactcgagga gatcggccag cttcccgact aggtccgcgc cggcagcgcg
     1921 accacctcgc acacgagccg cgttcgatcg acgtctgcgg ggtcgagttc gccctggccg
     1981 ggcgtgcgcg cgttggccgc gcctgtcgct ccagccacgc gaagggcatc ggcgggaccc
     2041 agtccccgcg atgacccggc aaggaacccg gcgaggaagg cgtccccgct gccgaccgag
     2101 tacggccccc ggtgtgcggc cggcaggttg cccagcgccc acgaaccgtc ggcggtcgcg
     2161 actgccgcgc cgtggacgcc ccgggtcacg atggcgttcg ccgcgccgcg cgagcggagc
     2221 tccgccgcgg cggtggccgc ccgatcgcgg gtccgcgcgc tggtccccgt gaccgtctcg
     2281 gcctcggtgg cgttgacctt gaccagccac ggccgggtca cgagcgccgc ggcgagcgag
     2341 tccccgtcgc tgtcgacgac cgcccgggct ccaagctcgg cggcgatgac ggcgagccgg
     2401 ccgtacgcgt cgacgggcgt gcccggcggc aggctgccgg ccagcacgac cacgacgtcg
     2461 ctcccgtcgt cgccgatcgc cgcccggagc gcgtcctcca cgcggggcca atcctcgttg
     2521 cgcagccgga tgccggcttc gtagaactcc gtgagttcgc ccgtggtctc gtccagcacc
     2581 gagaggcagg tgcgcgtctc gtccgtggcg gcgacctcgt gcagggcgat cgaccgctca
     2641 tcgagctgcg cccggtacca cgcacccgcg tggccgccga ggaccgcgac catcgtcgcg
     2701 tcgagcccca tgtgccgcgc cgcgcggacc acgttggccg ccttgccccc cgcgacgacc
     2761 gaccggatga ccggccgatg gatcgcgccg gcgacgagcc ggtcgaccga caccgtcttg
     2821 tcgatggccg cgttcagcgc cacaccgacg acgcgtcgga tcgccggcgg gtcgacggcc
     2881 gcggtcacgg tgcacccatc gcgagcaccg ccgcctccgc ctcggcccgc gtcggctgcg
     2941 agtccgtgcc tccggcggcg tgcgtggaca gcgatccgca cgcgac
//
LOCUS       DBNHXO010000192         2694 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5408624, whole genome shotgun sequence.
ACCESSION   DBNHXO010000192 DBNHXO010000000
VERSION     DBNHXO010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5408624"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="AC162A_04095"
     CDS             <1..412
                     /locus_tag="AC162A_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010052.1"
                     /translation="ALHDADMAPPANVGTWPMQASLASKGDWQGLMKYNNQAARSRAA
                     VAPAAAAPAAAAEPAQDLTQIKGIGPRIATILNDGGITNYGQLEHANGGDLRQIIATG
                     GALPPATIDSWPTQASYASRGDWAGLAAYNNSHH"
     gene            518..928
                     /locus_tag="AC162A_04100"
     CDS             518..928
                     /locus_tag="AC162A_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR5010053.1"
                     /translation="MVHDPTHDQDDATTRIGPAGAAIGQSPRMLRYLEDQGVIVAERR
                     PGPRGHRHYPPPEVALGRAASASMEAGHPTATLRALRALAERRVGDARASGDPLAWFE
                     LLALARAVEVSRRQDEAPARPPGPPGISRPPRGP"
     gene            925..2091
                     /locus_tag="AC162A_04105"
     CDS             925..2091
                     /locus_tag="AC162A_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter permease"
                     /protein_id="HIR5010054.1"
                     /translation="MTAHRTHAAGLVVATALGMAPLAAAPLGRLGSPFALLVVLPALA
                     LALDAYGWSQAAGAVIRRVPEPMARLLAAYAVWLATSALLTLDVAAAAGATVAAEVAG
                     PDRPAQRWHLGAAMLGANVGSLLFPFSNLTNLVVVGAAGIGLTAYVAAAWLPQVGAAA
                     ACGLLLAWRARRSGVLATVGVQPDAIDEPSARPAAPSGPSAWVGLIALAGGSGAVVAG
                     IAGADMAIPFALATAVVVGAGVADGRLTPSAVACSVPTGALALVAAAAVLNGPLAGAA
                     VALPWPPDDPLGVVVALIVGGLLAASVNNLPAAAVGAIWLAGSPVSIVVAYLVGTNVA
                     AIATPHGSAATMLVRSIATRREVPLPAIGHLRTAWRYALTGSVAAGGLLAAGIR"
     gene            2229..>2694
                     /locus_tag="AC162A_04110"
     CDS             2229..>2694
                     /locus_tag="AC162A_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5010055.1"
                     /translation="MPRLQAKSFGEPDDVRTMPLVRIETVAVDEASVGHCRFEPGWRW
                     SESMGPILGTPSCPMRHLGYTLSGVIHVVMDDGQALDIGPNDVFDIPPGHDKWVLGDE
                     PWVSIEWGGSGRAMREALQDAPGRMLATVVFTDIVDSTARLREAGDAAWRDQL"
BASE COUNT          340 a          998 c          985 g          371 t
ORIGIN      
        1 cgcgctgcac gatgccgaca tggcgccgcc cgccaacgtc gggacgtggc cgatgcaggc
       61 cagcctcgcc tccaagggcg actggcaggg cctgatgaag tacaacaacc aggcggccag
      121 gtcgcgagcc gccgtggcgc ccgcggcagc tgcgccggcc gcggccgccg agcccgccca
      181 ggacctcacc cagatcaagg gcatcgggcc gcggatcgcg acgatcctca acgacggcgg
      241 gatcaccaac tacggccagc tcgagcatgc caacggtggc gacctgcgcc agatcatcgc
      301 gaccggcggt gccctgcccc ccgccaccat cgacagctgg ccgacccagg cctcgtatgc
      361 cagccgtggc gactgggcgg gcctggcggc gtacaacaac agccaccact gacctccgct
      421 cggggtgcgg cctcgctcgg cggcgccgca tcccgatcag ccctcaccgt cgggcgccaa
      481 gtgtcgctat ctgccacgat cgtggtagat tcggcagatg gtgcacgatc cgacgcacga
      541 tcaagacgac gcgacgacgc gtatcggtcc cgccggtgcg gcgatcggac agtcgccccg
      601 gatgctccgc tacctcgagg accagggcgt cattgtcgcc gagcgccgac cgggaccgcg
      661 cggccatcgt cattacccgc cgcccgaggt cgcactcgga cgcgcggcgt cggcgtcgat
      721 ggaggccggc catcccacgg cgacgctgcg agcgctccgg gcgctcgccg aacgacgggt
      781 tggcgacgca cgagcgtccg gcgacccgct cgcctggttc gagctgctcg cacttgcgcg
      841 cgcggtggag gtgtcccgcc gccaggatga agcgccggcg cggccgcccg ggccacccgg
      901 catctctcgg cctccgcgcg ggccgtgacc gcacaccgga cgcacgccgc cggcctggtc
      961 gtggcgacgg cgctcgggat ggcgcccctg gcggccgccc cccttggacg cctggggtcg
     1021 ccgttcgcgc tcctcgtcgt cctgccggcc ctggccctgg cactggacgc gtacggctgg
     1081 agccaggcgg caggcgccgt catccgtcgc gtcccggagc ccatggcgcg cttgctcgcg
     1141 gcctatgcgg tctggctggc gacgagcgcg ctgctcacgc tcgatgtcgc cgccgcggcc
     1201 ggggcgaccg tcgcggccga ggtggccggg ccggaccgac cggcacagcg ctggcatctc
     1261 ggcgccgcga tgctcggggc aaacgtcggc tcgctgctgt tcccattcag caacctgacc
     1321 aacctcgtcg tcgtcggtgc cgcagggatc ggcctgacgg cgtatgtcgc ggcggcctgg
     1381 ctgccgcagg tcggggccgc tgccgcgtgc gggctgctgc tcgcgtggcg agcgcgccgt
     1441 tcgggagtgc tggcgacggt cggcgtccag ccggacgcga tcgacgagcc gtcggcccga
     1501 cccgccgcgc cgagcggacc gagcgcgtgg gtcggcctca tcgcgctggc cggtgggagc
     1561 ggtgcggtcg tggcgggcat cgcgggcgcg gacatggcca tcccgtttgc cctcgcgacc
     1621 gcagtcgtcg tcggcgccgg cgtcgcggac ggccggctga cgccctcggc cgtcgcctgc
     1681 tccgtcccga ctggcgcgct cgcgctggtc gctgccgccg ccgtgctcaa cgggcctttg
     1741 gctggcgcgg cggtggcgtt gccatggcca ccggacgacc cgctcggcgt cgtcgtcgcg
     1801 ctcatcgtcg gcggcctgct cgcggcgtcc gtgaacaact tgccggccgc ggcagtcggg
     1861 gccatctggc ttgcaggctc gcccgtctcg atcgtcgtcg cgtatctcgt cggcaccaac
     1921 gtggctgcca ttgcgacccc gcacggctcg gccgcgacga tgctcgtccg atcgatcgca
     1981 acgcgccgag aggtgccgct gcccgcaatc ggccacctcc ggactgcgtg gcggtacgcg
     2041 ctcactgggt cggtggccgc gggcggcctg ctcgccgccg ggatccggta gggcggatca
     2101 cgcgcgcaca gctggatcgc cgcgtgcggt ccatatatcc gccacagggc ggcgagatcc
     2161 atggctgaag cacagggaac tgggccgggg catcatggcc gcgtcaaccg tgggcacgga
     2221 gaccctcgat gccgcgactg caggcgaaga gcttcggcga acccgacgac gtccggacga
     2281 tgccgctcgt gcgtatcgag acggtcgcgg tcgatgaggc gagcgtcggg cactgccggt
     2341 tcgagcccgg ctggcgctgg tcggagagca tgggccccat cctgggcacg ccctcgtgcc
     2401 cgatgcggca cctcggctac acgctgtcgg gcgtgatcca cgtggtgatg gacgatggcc
     2461 aggcgctgga catcgggccc aacgacgtgt tcgacatccc gccgggccac gacaagtggg
     2521 tcctcggcga cgagccatgg gtgtcgatcg agtggggcgg cagcggacgg gcgatgcgcg
     2581 aggccctcca ggacgccccc gggcggatgc tcgcgaccgt cgtgttcacc gacatcgtcg
     2641 attcgaccgc gcggctgcgc gaggcgggcg acgccgcatg gcgcgaccag ctct
//
LOCUS       DBNHXO010000193         4147 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_51307, whole genome shotgun sequence.
ACCESSION   DBNHXO010000193 DBNHXO010000000
VERSION     DBNHXO010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_51307"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="AC162A_04115"
     CDS             <1..197
                     /locus_tag="AC162A_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010056.1"
                     /translation="MFVAIVDAVARLGDVQVSDPEVDVILVNRFYLRGVDQVDKRTGA
                     PHPKLPGAPLGGVSWQDRSR"
     gene            236..889
                     /locus_tag="AC162A_04120"
     CDS             236..889
                     /locus_tag="AC162A_04120"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5010057.1"
                     /translation="MFDIGETLVDENRLWLAWADWLGIQPLTFLAVCGAVIARGGDHR
                     EPFRILRPDIDLRAARLERETAGVPDVVEPRDLYPDAIGALRGLREAGFRVGVAGNQP
                     QRVEALFRDLGVTLDLIASSETLGVEKPDPAFFAAIGDRLGLAPGQIAYVGDRLDNDV
                     RPAAAAGMRAIFVRRGPWAWIQAGREPVPEAAAVIADLTTIVEVVRGLAGGKKSETG"
     gene            complement(886..2115)
                     /locus_tag="AC162A_04125"
     CDS             complement(886..2115)
                     /locus_tag="AC162A_04125"
                     /inference="COORDINATES: protein motif:HMM:NF018290.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE/TadZ family protein"
                     /protein_id="HIR5010058.1"
                     /translation="MESLAGHPAVAVFLPPTERELVAEELREGGFDPIVVHGPDELEG
                     VLATRRDLVVALIDLGADYDSGSACWTLLHAGGHNVPSLLVVDTASLDRLDVEAPGHE
                     NDEYLLRPYTAESIRWQIEAMCIRSVAVDDGSGPVLQGSIGQVEWGHRGQLILVFNPK
                     GGVGKTMVSTNLAAALVARGQRVLLVDADTVTGHVPTSLGMDAVPTVIDAWRDEMDGG
                     PMLTFGEMASVHSSGLKVLPLSAHPLNTELLEPQRVAGAIAVARRSFDFVIIDLHPSY
                     SPLNRAIFDRADRILVPVTPDLPAIRAVVKLREIADELGMRDRLSLIVNRANSGIKVD
                     DLERAIGMPTYAQVRSSGLLLVKASNEGRTLTEIAPKEKITQDFALMADRLLGLQPVE
                     VPKPQMNMFRRATAVRA"
     gene            complement(2118..3893)
                     /locus_tag="AC162A_04130"
     CDS             complement(2118..3893)
                     /locus_tag="AC162A_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010059.1"
                     /translation="MASSDTKSGFRLPWNSDRSPNEGTPEGAEATPEVVAEPAPASLE
                     WPDTNIDARSGTSTNGQQRPTQEAAPDAAATDAAATETQVPEESQRMVEIEVPSAPAA
                     APRKPTKLMADLSAAIRATAAAARDQAVQQLDADARQVTEKIREGATEGSATLRKRSE
                     DDVAGIRDWSKAEIARIREETDNRIATRKQVLEGELASHADAVNNRVEEVQSEVDRFK
                     ADMEEYFDRLGNEEDPSRLATMVEAMPDAPSFDAWADLGEAEDLVPEPEARVEDAYVA
                     ADAVAELEPVEAIAETSDDDAPADEAIDGAAGDEVAQVVADEVVAETAIVEDAVTDVA
                     SDEAEQVVAEATAEPETSNDDAAKPAAKAVTWGEGDGDWRGSGDAAATPDDGSAVWAA
                     GEVPEGFPTGAEGGEAVDRGAIMAALEAAAEAVVAAEAAADSADQAEAAADVAETAAE
                     LVVGRNAGEDGSLDPEAQAALDARVDAGGFEGSYADRLAHLLPTTPEAGADAEPKTTQ
                     VVVTGLVSVASIASFKRHLGRLSGVKTVAVASGPEGEFVFNVTHRPDVSFRDAIPSMQ
                     GFAARVTGQSEGVIQVTARDPESEN"
BASE COUNT          524 a         1398 c         1568 g          657 t
ORIGIN      
        1 agatgttcgt cgcgatcgtc gacgcggtcg cacgcctggg cgacgtccag gtcagcgacc
       61 ccgaggtcga cgtgatcctc gtgaaccggt tctacctgcg gggcgtcgac caggtcgaca
      121 agcgcaccgg cgcgccgcac ccgaagctgc ccggggcgcc gctcggtggc gtgagctggc
      181 aggaccgctc ccgctgatcc ggtcgtcggg gggcggtgag cccgtccgtg ccgtcgtgtt
      241 cgacatcggc gagacgctgg tcgacgagaa ccgtctgtgg ctcgcgtggg ccgactggct
      301 gggcatccag ccgctgacgt tcctcgctgt ctgcggggcc gtgatcgctc ggggcggcga
      361 tcaccgcgag ccgttccgga tcctgcggcc ggacatcgac ctgcgggcgg cgcgcctcga
      421 gcgtgagacg gccggcgtcc ccgatgtcgt cgagccacga gacctctacc cggacgcgat
      481 cggggcgctc cgcgggcttc gggaggcggg ctttcgagtc ggcgtcgcgg gcaaccagcc
      541 ccagcgcgtc gaggccttgt tccgcgacct cggcgtgacg ctcgacctga tcgcttcgag
      601 cgagaccctg ggcgtcgaga agccggatcc ggcgttcttc gcggcgatcg gggaccggct
      661 cggcctcgcg ccgggccaga tcgcctacgt gggcgaccgg ctggacaacg acgtgcggcc
      721 ggcggccgcg gcgggcatgc gcgcgatctt cgtgcgccgt gggccgtggg cgtggatcca
      781 ggcggggcgt gaaccggtgc ccgaggcagc ggcagtcatc gctgacctga cgacgatcgt
      841 cgaggtggtg cggggcctcg cgggcggcaa gaaatccgag acgggttagg cgcggacggc
      901 ggtggcccgc cggaacatgt tcatctgcgg cttcgggacc tcgaccggct ggagcccgag
      961 caggcggtcg gccatcaggg cgaagtcctg cgtgatcttc tccttgggcg cgatctcggt
     1021 cagggtccgg ccctcgttgc tggccttgac caggagcagc ccgctggagc ggacctgggc
     1081 gtaggtcggc atgccgatcg cgcgctcgag gtcgtcgacc ttgatgccgc tgttggcgcg
     1141 attcacgatc agcgacaacc ggtcgcgcat gcccagctcg tcggcgatct cgcgcagctt
     1201 gaccacggcg cggatggccg gcaggtccgg cgtgacgggg acgaggatcc ggtcggcccg
     1261 gtcgaagatg gcccggttga gcgggctgta cgacggatgc aggtcgatga tcacgaagtc
     1321 gaagctgcgc cgggcgaccg cgatcgcgcc ggcgacgcgc tgcggctcca gcagctccgt
     1381 gttcagcggg tgggcggaga gcggcaggac cttgaggccc gagctgtgga ccgaggccat
     1441 ctcgccgaac gtgagcatcg ggccgccgtc catctcgtcc cgccaggcgt cgatcaccgt
     1501 cgggacggcg tccatgccca gcgacgtcgg gacgtgtccg gtcacggtgt cggcatcgac
     1561 gaggagaacg cgctggccgc gcgccacgag cgctgccgcg aggttggtcg agaccatcgt
     1621 cttgccgacg ccgcccttgg ggttgaacac gaggatcagc tgccctcgat ggccccactc
     1681 gacctggccg atgctgccct ggaggaccgg accgctccca tcgtcgacgg cgacggaccg
     1741 gatgcacatc gcctcgatct gccagcggat cgactccgcc gtatacggcc gaaggaggta
     1801 ctcgtcgttc tcgtgcccgg gggcctcgac atcgaggcga tcgagggagg cggtgtcgac
     1861 gacgagtagc gacggcacgt tgtgcccacc ggcgtgcagg agcgtccagc aggcgctgcc
     1921 tgaatcgtag tcggcgccga ggtcgataag ggcgaccacg aggtcgcgcc gggtcgccag
     1981 aacgccctcg agctcgtcgg gtccgtggac cacgatggga tcgaacccgc cctctcgcag
     2041 ctcctcggcg acgagttcgc gctcggtcgg cggaaggaag accgcgacgg cggggtggcc
     2101 cgccagggac tccacgatca gttctcggat tcgggatcgc gtgcggtgac ctggatgacg
     2161 ccctcgctct ggcccgtgac gcgggcggcg aagccctgca tcgacgggat cgcatcgcgg
     2221 aacgagacgt cagggcgatg ggtcacgttg aacacgaatt cgccttccgg accggacgcg
     2281 acggcgacgg tcttcacgcc ggacagccgg ccgaggtgtc gcttgaagct cgcgatgctc
     2341 gcgacggaga cgaggcccgt gacgacgacc tgcgtggtct tgggctcggc gtccgcgccg
     2401 gcctcgggcg tggtgggcag gagatgggcg agacggtcgg cgtacgagcc ctcgaacccg
     2461 ccggcgtcga cgcgggcgtc aagcgcggcc tgggcctcgg gatcgagcga gccatcctcg
     2521 ccggcattgc gaccgacgac cagctcggcc gcggtctcgg caacgtcggc agcggcctcg
     2581 gcctggtcgg cggagtcggc ggcggcctct gcggcgacga cggcctcggc tgcggcttcg
     2641 agggccgcca tgatggcgcc gcgatcgacg gcctcaccgc cctcggctcc ggtcgggaac
     2701 ccttccggga cctcaccggc ggcccagacc gcgcttccgt cgtcgggcgt cgcggcggcg
     2761 tcgccggagc cacgccagtc gccgtcgccc tcgccccagg tgaccgcctt ggcggccggc
     2821 ttggcggcgt cgtcattgct tgtctcgggc tcggcggtgg cctcggccac gacctgctcg
     2881 gcctcatcgg acgcgacgtc ggtcacggcg tcctcgacga tggcggtttc ggcgacgact
     2941 tcgtcggcca cgacctgcgc gacctcgtcg ccggcggcgc catcgatggc ctcgtcggcc
     3001 ggggcgtcgt cgtcgctggt ctcggcgatg gcctcgaccg gctcaagctc ggcgacggca
     3061 tcggcggcga cgtaggcgtc ctcgacgcgt gcctcgggct cgggcacgag atcctcggcc
     3121 tcgccgaggt cggcccaggc gtcgaacgag ggcgcgtcgg gcatcgcctc gaccatcgtc
     3181 gccaggcggg acgggtcttc ctcgttgccc aggcggtcga agtattcctc catgtcggcc
     3241 ttgaagcggt cgacctcgga ctggacctcc tcgacgcggt tgttgacggc atccgcgtgc
     3301 gaggcgagct cgccctcgag gacctgcttg cgggtcgcga tgcggttgtc ggtctcttcg
     3361 cggatccggg cgatctcggc cttcgaccag tcgcggatcc ccgcgacgtc gtcctcggag
     3421 cgcttgcgga gcgtagcgga gccctcggtc gcaccctcgc ggatcttctc cgtgacctgg
     3481 cgcgcgtcgg catcgagctg ctgcacggcc tgatcgcggg cggcggctgc cgtcgcgcgg
     3541 atcgcagccg acaggtcggc catgagcttg gtcggcttcc tcggcgccgc ggccggggcg
     3601 gaggggactt cgatctcgac catgcgctgg gactcctcgg gaacttgggt ctcggtcgcg
     3661 gcggcatcgg tcgcagcggc gtctggcgcg gcttcctggg tcgggcgctg ctgcccgttg
     3721 gtgctggtgc ccgaccgggc gtcgatgttg gtgtcgggcc actcgaggct ggcaggcgcg
     3781 ggctctgcga cgacctcggg ggtcgcctcg gcgccctcgg gcgttccctc gttgggagag
     3841 cggtcagagt tccacgggag ccggaaacca cttttggtgt cgctggaggc catgagcaga
     3901 gacttcctct acggggtggg cacgcaaccg caggtgctgc tggggggaca gtaggcggca
     3961 tggtgggctg tggtgcagtc gctagaggta ccagcgaggc attccggtgg taccggcgcg
     4021 ggtggccggg tcgcgatcca ctgagcgttc cggacggccc gcgtggtgcc cgcccgcaca
     4081 ggaattcgcg gtgacagggt ggtgcccgtc agacgctcgt ctccgggcct cgttcccgcg
     4141 gctcgtg
//
LOCUS       DBNHXO010000194         1955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5012537, whole genome shotgun sequence.
ACCESSION   DBNHXO010000194 DBNHXO010000000
VERSION     DBNHXO010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5012537"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="AC162A_04135"
     CDS             <1..456
                     /locus_tag="AC162A_04135"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR5010060.1"
                     /translation="DVDLFTDGAPKAAQNFVDLANKGFYDDVIFHRVIPGFVIQGGDG
                     EHGKKASLNAGRVGTGGPGYKFADEPITGDYYRGTLAMANSGPNTNGSQFFICHQDLT
                     GKLPKNYTIFGQVTKGLDVVDQIATAPRNSRDLPDSPVAIKTVTILDEA"
     gene            complement(889..1020)
                     /locus_tag="AC162A_04140"
     CDS             complement(889..1020)
                     /locus_tag="AC162A_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010061.1"
                     /translation="MQDEHSFTRRSVADLRLSVGTALDLANQSRGECSASSGAFFLA"
     gene            1161..>1955
                     /locus_tag="AC162A_04145"
     CDS             1161..>1955
                     /locus_tag="AC162A_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018586551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5010062.1"
                     /translation="MGRYLDWLAAERGIDLATYDDAWRWSVDEPGAFWQSIWDHFGVR
                     SATPLGPALADARMPGARWFPDAKLNYAERALALPGRAADDVVILGRSQTRDPRDLTA
                     AELRDAVARCRAGLTRLGVRRGDRVAAFLPNVPEAVIGLLATASLGAIWSSCAPEFGT
                     KAVVDRLAQIEPVVLLTIDGYRYGDRDIDRSNAIADIRAALPTLRATVVVPYLRPEAD
                     AVDVIPGAVSWSSLLADTAPLAFDPVPFDHPLYVLFSSGTTGLPKPI"
BASE COUNT          316 a          683 c          644 g          312 t
ORIGIN      
        1 gacgtcgacc tgttcaccga tggcgccccg aaggccgccc agaacttcgt cgaccttgcc
       61 aacaagggct tctacgacga cgtgatcttc catcgcgtca tcccgggctt cgtcatccag
      121 ggtggcgatg gcgagcacgg caagaaggcg tcgctcaacg cgggccgggt cggaacgggc
      181 ggccccgggt acaagttcgc cgacgagccg atcaccggcg actactaccg cgggaccctc
      241 gcgatggcga actcggggcc gaacaccaac gggtcgcagt tcttcatctg ccaccaggac
      301 ctgaccggga agctgcccaa gaactacacg atcttcggcc aggtgacgaa ggggttggac
      361 gtcgtcgacc agatcgcgac cgcgccgcgg aacagccgcg acctgcccga ttcgccggtc
      421 gcgatcaaga cggtgacgat cctcgacgag gcgtgaactc cgtcgattga cacacaatcg
      481 ttaagggata ttcacccaag cgacgactca ggcaccttcc ccaatcccga cctgctcgct
      541 aggattgccg tgctgccagt cagcaagcgc tgccccagca tcaggatcat gggtaggttg
      601 ggttagggta gtcggttaag ccgacgcgat agatgatcct gaagcgtggc agaaggtccg
      661 ccgcagggtg gacaccttca ccgggacagc aacgaggcgg ctcctcacgg agccgcctcg
      721 tagtttgtct ggggcctacc gtcccgttgc ctcccccaag ttggtcatcg agccatcggg
      781 tgaatgcact tgtcggccgg accccgccga ccctgacatg tacgcatgcg gtgaatgcga
      841 aagggctgga tcggcccgcg acctgcccga acattcatcc cacgagtgct aggccaggaa
      901 gaacgctccg gagctggccg agcattcacc ccgcgactgg ttggccaggt cgagggcggt
      961 ccccacgctc agccgcaggt ccgcgaccga acgccgcgtg aacgagtgct cgtcttgcat
     1021 tcacgccatg agtacaagac agggtgacgc cgtgagggcg tcgtcgcggg atgcccgaca
     1081 atcgagtcgt gacccatcag cgcgagctct cgcccgagat cctgtggtcg ccgccggccg
     1141 acgcgcgcga acggtcgagg atggggcggt atctcgactg gctcgcggcg gagcgcggca
     1201 tcgacctcgc cacctacgac gacgcctggc gatggtcggt cgacgagcct ggtgcgttct
     1261 ggcagtcgat ctgggaccac ttcggggttc gctcggccac gccgctgggg cccgcgctgg
     1321 cggatgcgcg catgcccggt gcccgctggt tccccgacgc gaagctgaac tacgccgagc
     1381 gcgcgcttgc cctgcccggc cgagcggccg acgatgtcgt catcctgggc cgctcgcaga
     1441 cgagggaccc gagggacctg accgccgccg agctgcgtga cgccgtcgca cgatgccggg
     1501 ccgggctgac gcgcctcggc gtccggcgtg gtgaccgcgt cgcggcgttc ctgcccaacg
     1561 tccccgaggc cgtgatcggc ctgctcgcga cggcgtcgct cggcgcgatc tggtcgtcgt
     1621 gcgcgcccga gttcgggacc aaggccgtcg tcgaccgcct ggcgcagatc gagcccgtgg
     1681 tcctgctgac catcgatggc taccgttatg gggaccgcga catcgaccgg tcgaacgcca
     1741 tcgccgacat ccgagccgcg ttgccgaccc tccgggccac cgtcgtcgtg ccgtacctgc
     1801 ggccggaggc ggacgccgtc gacgtgatcc cgggcgctgt gtcgtggtcg tcgctcctgg
     1861 ccgacaccgc gccgctcgcc ttcgaccccg tcccgttcga ccacccgctg tatgtcctgt
     1921 tctcgtcggg cacgaccggc ctgcccaagc cgatc
//
LOCUS       DBNHXO010000195        13705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4219245, whole genome shotgun sequence.
ACCESSION   DBNHXO010000195 DBNHXO010000000
VERSION     DBNHXO010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 13705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13705
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4219245"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="AC162A_04150"
     CDS             <1..817
                     /locus_tag="AC162A_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024122.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HIR5010063.1"
                     /translation="LPDPPRSSVLPPAPVPPLRPGQPPLAILVDYDGTIARTDVSDTL
                     MAEFVTADWESHVADYDAGRTGSRRLMAWEVGQITADPDALRAKAASQPHDPSFRPFA
                     ERALAAGIPVEVVSDGFGFFIEPALAALGVGQLPVVTAATTFEAGGPRIEFPNGNPDC
                     FVCGTCKRNRVLAHQAAGRAVVFIGDGPSDRYAAGYSDVVFAKESLEQICLNESWAYR
                     RWTEFSEIDAWLAHTLAAFADDPSSLPGPTHRPLFCGAEVWGPGRFDPAPLED"
     gene            904..1464
                     /locus_tag="AC162A_04155"
     CDS             904..1464
                     /locus_tag="AC162A_04155"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5010064.1"
                     /translation="MDRRELVERAGRGDHDAFTVLATAASSRLDAAARLILRDRELAR
                     DAVQNCLVRAWRDLPGLRDPDRFDAWLHRLLVNACLDEARRRRRRPIEVEIQPIDDPT
                     TNDDARRLADRDLVEQALARLDPEQRALVVMHYYLGYPLPDAARSLGISLPAAKSRLH
                     RAMGALRRVLVADPVDGIDLEGGRTA"
     gene            1461..2762
                     /locus_tag="AC162A_04160"
     CDS             1461..2762
                     /locus_tag="AC162A_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5010065.1"
                     /translation="MTAFDGFDLVDARVVGALDEIAAATRPDYLDDIFQVTARTRQRP
                     RWTFFERWLPMDTTATQPVGRIRTPLRPIALLLILGLLAIVATAFIVGSQRHSLDPYG
                     PAKNGVIAYGADGDLFVRDSLTGAPRLLAGGPDVQGGALFSRDGQLVAFDNVVGDVDH
                     VMVAWADGSNPHRILAEPFLNPTAAWSPDSRSLVLTNESPNGDRTLWIAPADGSGARQ
                     IDLGNLVPYDAIYNPSNDGTILLRADSVVDGTDLYLIDETGRTVRKYNLPGTMLYGAQ
                     WELAGAAFSPDGKTIAHNAVEDIPGDTARFRTYLVDVDGTNQRELPAPPNAPVNYSQA
                     WPVFSPDGKWIIMESWVGAPGGPSTNQIAIVPADGSAPARGIGPSLVNQGLLRDWSPD
                     GTKVLVGVIDGNDTYEVDPVTGEYSKLPWTGDMPSWQRTAR"
     gene            2810..3832
                     /locus_tag="AC162A_04165"
     CDS             2810..3832
                     /locus_tag="AC162A_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010066.1"
                     /translation="MSRHARNLLATTILALVITALVAGPTAATLPGSNGRITFMRFEA
                     DHWQVWVSKPDLSDAVQITGGASESVCPVWSPDGRRIAFDSNRSDPDPTDEVGINDVF
                     TMRPDGSDVRRVTDSHGFSGEPAWSPDGSLIAFSSDRGISPLRQAIYVIRPDGTEMRR
                     VTEKPRGGNWQGSPRFSPDGRKLVFTEFRPGDGEAGVETSALFTINLDGTRLRRITPW
                     ATHPGDADWSPDGKRIVFETIFEFPSRAASVWIVAADGGHLRQLTFDRVNFDDPETFL
                     FEGSFDPVWSPDGRRILFSHDEYGPEDGSTGLQTMKPDGSGRTWVSTVHGAEHQVDWA
                     SVRPRR"
     gene            complement(3859..4446)
                     /locus_tag="AC162A_04170"
     CDS             complement(3859..4446)
                     /locus_tag="AC162A_04170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR5010067.1"
                     /translation="MEFTDVVMKRRAVRRFEEGGVSREVIERIARLAQRTPSAGFSQG
                     QRLVVVTDPARRRDVARICGEEEYEPDFGPWISECGAQFIPCVSEAIYHRRYQEPDKV
                     QEDGSEIEWPVPFWWIDIGATMQNIMLGAVNEGLGCGFVGPEIDALRAYLGIPDEFTP
                     IGVMPVGRPLPDVRSPSLKRGWVPFEQFARWEAWA"
     gene            4686..5234
                     /locus_tag="AC162A_04175"
     CDS             4686..5234
                     /locus_tag="AC162A_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5010068.1"
                     /translation="MRDIVEQAMRGDREAFGWLVGQTSDRMYAIATRILRDSDLAEDA
                     LQGALINAWRQLPTLRDPDRFEAWVRKLLVHACYAEARRRRTWTANVRVLPVDGPAAP
                     DASISVVDRDALDRAFRRLTVEQRAVFVLHHHLGLPLVEIAETLGVPAGTARSRLHYA
                     TRALRAAIEADAAPVVPEGRMA"
     gene            5231..6520
                     /locus_tag="AC162A_04180"
     CDS             5231..6520
                     /locus_tag="AC162A_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010069.1"
                     /translation="MTKDRDFDRIARAWLDLMPDEAPDRTVAAVLHAVDATPQVRRTW
                     GRLTWRSIAMNRTAFVLGAAAIVVAAGALVMLRTGPTSNVGSPPTPSATIPPSPSPAG
                     AAAPVPGELQAIWMGEHRDLVAPDAGSLIEFRENTFAHAQSAGSSVQLLQSTAAATGD
                     RQLRLVSAAAGDGCEQGDIGTYSWSLNPSGRNLTVTHELDACQARADAIAGDWWLMGC
                     TTPDDNCLGALDAGTFSSQFITPRLKPGESWSPVFGALTYTVPDGWANASDWPESFEL
                     VPASELPPIDEANRRRHINLLTRPSAMTQDKPCSDMVEPGVGRTIDDIVTWLGTVPGL
                     ITTAPTAITIDGHPGQWLDVRLDPAWDKTCDGGSGPMVAYLNPGIAISSDERERVLLV
                     DLGDDDVIAIAVWARDKAAFDSFIPEAMTVIESFTFK"
     gene            6744..7301
                     /locus_tag="AC162A_04185"
     CDS             6744..7301
                     /locus_tag="AC162A_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5010070.1"
                     /translation="MTVDRDLVEAARHGDQGAFMDLVRSRGGRLFAIAHRILRDVDLA
                     EDALQDALVIAWRDLPSLRDPDRFDPWVHRLLTNVCVSEATRERRRTANLRVLPLDGP
                     SAPDDLIGVVTRDQLDQGFRRLTPDERAVLVLHHYVGYAPTEIAEMLGVPPGTIRSRI
                     HHAHRAMRAALDAEARNAKAGGRTA"
     gene            7298..8491
                     /locus_tag="AC162A_04190"
     CDS             7298..8491
                     /locus_tag="AC162A_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010071.1"
                     /translation="MTEHTDIDRLLETWFDDGPTAMPDRVVTVVADRIGRQGQRHTWL
                     LDRRPKVNLFLKLAVAAALGAAVLGGAIFIAGRQTQTPTPTPIPAPSSSPGASAAAAG
                     SPVDDALRATWKAFAGENPVLQTGAGPVSLQFNPLGTGIEATNFGPGHGYASTANETG
                     ADRIEVVLDRSGGGCTAGARGVYRTLLSDDRSELTLISESEDCSNRGIVLGRAWARSL
                     ADSSLVGAGVVDAIAPAFKVALPDDSYETRTLPDFVEIGGTNGSLMVFKNPQPFVDAC
                     STDEERVPWQPGAAAFIDAFRANDAFEVSESTPLTISGHDAVHVQIGGKADYARCPGQ
                     ELYEYTPKDCECHFVVGQGFADSMYLVDVDGDTFMFIISPLGTPESEWPIVESIEIPY
                     EIPSR"
     gene            8557..9603
                     /locus_tag="AC162A_04195"
     CDS             8557..9603
                     /locus_tag="AC162A_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5010072.1"
                     /translation="MVRRARRLLAAGAILVALVMTTAGPASATLPGPNGRITFMRFDA
                     DGQFQVWVANPDMSTQVQLTSGPSDAWFPAWSPDGRRIAFASHRTDPDPTDDVEMMDV
                     FTMRPDGSDVRKVTDSRGFSGTPSWSPDGRWLVFSADRADYPRGQGIYIMRSDGSSAP
                     RRITTLPSGSVWQELARFSPDGSRIVFSEYRNVSVPGDDGESVDVEQSALLTVRPDGT
                     HLRRITPWELSASDGDWSPDGRRIVFGTRPRWAGGLQEVMIADADGGHLRNLSRDHPV
                     FGPPLPEDDYRESFNPAWSPDGRLVVFVHAKWNPTDGFRMGLQTIRPDGSHRGWLSQG
                     EEHQPDWGSARPIR"
     gene            9763..10305
                     /locus_tag="AC162A_04200"
     CDS             9763..10305
                     /locus_tag="AC162A_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5010073.1"
                     /translation="MSELSTTGPVPASTVEAAVAGDAVAFARIVRAHHDDMARVCRIV
                     CGDPDLAQDAVQSAWPIVWRKLRTLREPERLRPWLVSVAVNEARQLLRREHRDRVTAI
                     EVADVGSDRSDPAARSADQDLLAAVRSLPADERTLLALRYVAGFDATEIGSAMGMSAS
                     GVRSRLSRLIERLRTEIGDD"
     gene            10298..11614
                     /locus_tag="AC162A_04205"
     CDS             10298..11614
                     /locus_tag="AC162A_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kelch repeat-containing protein"
                     /protein_id="HIR5010074.1"
                     /translation="MTDRFEARLSRLLLVEAEDVIRPIDAGAMAAAAMGEVPMRRGMP
                     MVGTRAARALVLAGLLTLGLLATIALLAGSQRGPRPFAGVLDPTGPMTDGMLQPATLA
                     RPDGTVLVAGATADMRLHMESFDAATGRFTVLPGLDDRRFSHGRDELGLVPLADGTVL
                     VVMASASSQSGATEGGYALRYDLATREVRETAGTFAQTDPRRLVGEFRFASWPWSRRA
                     DGTIAWTPPTWIMSDVPVYDPRLNAFSVEPGPASPFTSIPPEADPFATDLYLGPSAIV
                     HLRDGRLLLLGASRDSNAPAAMVDGATFAGHPLDWPLVRGGTYAGVVLPDGRVLIAGP
                     TSHVFDPSTDKLVDLDVVDALSPAVQLRDGRVLLVGEAPTSLGSPARYRLWQFDPATT
                     TLERVGRGDAPAPTSGWTELSDGQVLVVSGYAGTGDALPRDAWILR"
     gene            11870..13582
                     /locus_tag="AC162A_04210"
     CDS             11870..13582
                     /locus_tag="AC162A_04210"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR5010075.1"
                     /translation="MAKYVFVTGGVTSSLGKGITAASIGRLLKARGLKVSVLKLDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDENLSQSSNVTTGRIYQAVIAKE
                     RRGDYLGGTVQVIPHITNEIKERITRVARAGDPDVVIVEVGGTVGDIESLPFLEAIRQ
                     MRKDQGRANVLYVHVTLLPALAATGELKTKPTQHSVKELRGIGIQPDVIVLRSDSEVP
                     DEIREKIALFTDVDVDAVIPAATAETIYEVPLQFEAFGFGRLLVRELGLGDADVVPDL
                     DGWRALVERIKAPKATLEIALVGKYIELPDAYLSVTESLKHAAWASGVDARVRWVDSE
                     QLTADNVAERLDGAAGVLVPGGFGHRGIEGKVLAAHWARERGVPYLGLCLGLQCAVIE
                     FAREIIGTGDVNSTEFDMFTTNPVIDFMPDQRDLEDKGGTMRLGLYPAKLLPGSKARA
                     VYGQEVVYERHRHRFEVNNRYRQVLEGAGMLLSGVSPDGRLVEIVELRDHPWFVASQF
                     HPEFKSRPERPHPLFDGFVTAAIAVGEGREPVFHAREPQIEQGREPDTGATSADAIES
                     IRGA"
BASE COUNT         2021 a         4987 c         4637 g         2060 t
ORIGIN      
        1 cctgcccgac ccgccccggt cgtccgtcct cccgccggcg ccggtgccgc cgctgcgacc
       61 cggccagccg ccgctcgcga tcctcgtcga ctacgacggc acgatcgcgc gcaccgacgt
      121 ctcggacacg ctgatggccg agttcgtgac cgcggactgg gagtcgcacg tggccgacta
      181 cgacgcgggt cgcacgggct cgcgacgcct gatggcctgg gaggtcgggc agatcacggc
      241 cgatccggac gccctgcggg cgaaggcggc gtcgcagccg cacgatccgt cgttccggcc
      301 gttcgccgaa cgagcgctcg cggcggggat cccggtggaa gtcgtgtcgg acgggttcgg
      361 gttcttcatc gagccggcgc tggcggccct cggggttggg cagctgcccg tggtcacagc
      421 ggcgacgaca ttcgaggccg gcgggccccg catcgagttc cccaacggca accccgactg
      481 cttcgtgtgc gggacctgca agcgcaaccg ggtgctcgcc caccaggccg cgggtcgggc
      541 cgtcgtgttc atcggggacg ggccgtcgga ccgctatgcg gccggctaca gcgacgtggt
      601 cttcgcgaag gagtcgctcg agcagatctg cctcaacgag agctgggcgt accggcgctg
      661 gaccgagttc tccgagatcg acgcgtggct cgcgcacacc ctggcggcgt tcgcggacga
      721 cccgtcgtcg ctgcccggcc cgacccatcg cccgctgttc tgcggcgccg aggtctgggg
      781 ccccggtcgc ttcgaccccg cccccctcga ggactagccc gcgatccggg agaaacgaat
      841 cgggttgcgg tgacgccata ccctgcagac ccggcctcgt gcgccgggcg agggtgaggg
      901 accgtggacc gacgggagct cgtggagcgg gcggggcgag gcgatcacga cgccttcacc
      961 gtgctcgcga ccgcggcgtc ttcccggctg gacgcggccg cgcgcctcat cctccgggac
     1021 cgcgagctcg cgcgcgacgc ggtccagaac tgcctggtgc gtgcctggcg ggaccttccc
     1081 gggcttcgtg acccggatcg gttcgacgcc tggctccacc gcctcctcgt gaatgcctgc
     1141 ctcgacgagg ctcgccggcg ccgccggcgg cccatcgagg tcgagatcca gcccatcgac
     1201 gacccaacca ccaacgacga tgcccgccgc ctggccgatc gcgacctggt cgagcaggcg
     1261 ctcgcgcgcc tcgatccgga gcagcgggcg ctcgtcgtca tgcactacta cctgggctac
     1321 ccgttgcccg acgccgcgag atcgctgggg atctccctcc ccgccgcgaa gtcacggctt
     1381 caccgggcga tgggtgcgct gcgccgggtc ctcgtcgccg accccgtcga cggcattgac
     1441 ctggaaggag gccgcacggc atgaccgcct tcgacggctt cgacctcgtt gacgcccgcg
     1501 tcgtcggcgc tctcgacgag atcgccgcgg cgacccgccc cgactacctc gacgacatct
     1561 tccaggtcac agcccggaca cgtcagcgcc cgcgctggac ctttttcgaa aggtggctcc
     1621 ccatggatac cactgcgacc cagcctgtgg gtcggatccg taccccgctc cgtccgattg
     1681 cgctcctgct gatccttgga ttgctggcca tcgtggcgac ggccttcatc gttgggtcgc
     1741 agcggcactc gctcgaccca tacggaccgg cgaagaacgg tgtcattgcc tatggcgctg
     1801 atggcgacct gttcgtccgc gactcgctca cgggcgcccc ccgactcctt gccggtggcc
     1861 cggacgtcca gggcggcgcc ctgttctccc gcgacggtca gctcgtcgcg tttgacaacg
     1921 tggtcggcga tgtcgaccac gtgatggtgg catgggccga cggctcgaat ccccacagga
     1981 tcctcgcgga gccgttcctc aacccgaccg ccgcttggtc gccggacagc cggtctctag
     2041 tgctcaccaa cgagtcgccc aacggcgacc ggacgctctg gatcgcgccc gccgacgggt
     2101 cgggtgcccg gcagatcgac ctcggcaact tggtgccgta tgacgcgatc tacaacccat
     2161 cgaacgacgg caccatcctg ctccgcgccg actcggtggt ggacggcacg gacctctacc
     2221 tgatcgacga gaccgggcgc accgttcgca agtacaacct cccgggaacg atgctctatg
     2281 gcgcccagtg ggagctggcc ggggccgcct tctcgccgga cggcaagacg atcgcgcaca
     2341 acgccgtcga ggacatccct ggtgacaccg cccggttccg cacctacctc gtcgacgtcg
     2401 acgggacgaa ccagcgcgag ctcccggccc cgccgaatgc ccccgtcaac tacagccagg
     2461 cgtggcccgt gttctcaccg gacggcaagt ggatcatcat ggagtcgtgg gtcggagccc
     2521 ccggcggccc gtccaccaac cagatcgcga tcgtgccggc cgatggctcg gcaccggccc
     2581 gaggaatcgg tccgtcgctc gtcaaccagg ggctgctgcg ggactggtca cccgatggca
     2641 cgaaggtgct cgtgggcgtc attgacggca acgacacgta tgaggtcgac ccggtcacgg
     2701 gggaatactc gaagctcccc tggaccggcg acatgcccag ctggcagcgc accgcgcgct
     2761 agccgagacc actgcgggag ccgcccaccg ccttcaggag gacctctcca tgtcccgcca
     2821 tgcccgcaac ctgctcgcca ccacgatcct ggccctcgtc atcaccgccc tggttgccgg
     2881 cccaaccgcg gcgacccttc ccgggtccaa cggccggatc acgttcatgc ggttcgaagc
     2941 ggatcactgg caggtgtggg tctcgaagcc cgatctgtcg gacgccgtcc agatcacggg
     3001 cggggcgtcc gagagcgtgt gtcccgtctg gtcgcccgac ggccgccgga tcgccttcga
     3061 ctcgaaccgg tccgacccgg atcccaccga cgaggtcggc atcaacgacg tgttcacgat
     3121 gcgcccggac gggtcagacg tcaggagggt caccgactcc cacggcttct cgggcgagcc
     3181 cgcatggtcg ccggacggct cgctcatcgc gttcagcagc gaccgcggca tcagcccgct
     3241 gcgccaggcc atctacgtga tccggccgga cggtacggag atgcgccggg tgaccgagaa
     3301 gccgcgggga ggcaactggc aaggatcgcc acgcttctcg cccgacggca ggaagctcgt
     3361 gttcacggag ttccgcccgg gcgacggtga ggccggcgtc gagacgtccg cgctgttcac
     3421 gatcaacctc gacggcacca ggctgcgccg gatcacgccc tgggcgacgc acccgggcga
     3481 cgcggactgg tcgcccgacg ggaagcgaat cgtgttcgag acgatcttcg agttccccag
     3541 ccgcgccgcg agcgtctgga tcgtcgccgc ggacgggggc cacctcaggc agctcacctt
     3601 cgacagggtc aacttcgacg accccgagac gttcctgttc gagggctcct tcgatccagt
     3661 gtggtcgccg gacggccgcc ggatcctgtt cagccacgac gagtacggcc ccgaggacgg
     3721 atcgaccggc ctccagacga tgaagcccga cggctctggc cggacgtggg tctcgacggt
     3781 ccacggggcc gagcaccagg tggactgggc gtcggtccga ccgcggcgct gacgcgcgca
     3841 tgggctagcg aggtcctctc aggcccaggc ttcccagcgg gcgaactgct cgaacggcac
     3901 ccagcctctc ttgaggctgg gtgaccgcac gtccggcagt ggccgaccga ccggcatgac
     3961 gccgatgggt gtgaactcgt cggggatccc gaggtaggcg cgcagcgcgt cgatctcggg
     4021 cccgacgaac ccgcagccga ggccctcgtt cacggcgccc agcatgatgt tctgcatcgt
     4081 ggcgccgatg tcgatccacc agaacggcac cggccactcg atctccgagc cgtcctcctg
     4141 gaccttgtcc ggctcctggt agcggcggtg gtagatcgcc tcgctgacgc acggaatgaa
     4201 ctgcgcgccg cactcgctga tccacgggcc gaagtccggc tcgtactcct cttcaccgca
     4261 gatgcgcgcc acgtcgcgcc ggcgcgccgg gtccgtgacg acgaccagcc gctgcccctg
     4321 gctgaagccc gccgacggcg tccgctgcgc gagcctcgcg atccgctcga tcacctcgcg
     4381 gctgactccg ccctcctcga accgacggac ggcgcggcgc ttcatgacga cgtcggtgaa
     4441 ctccatgggt cctcccagtc ttcgagggat caatcctcgc aggtcccgca tcatggctcg
     4501 gtaccctcgt cccggtcgcc caccgttcgg gcgttcgccc tacatagcgc gctgctcccg
     4561 gaagccatgc cgatcgtccc gagcttccgc ttcgcgccgt gacccgtgaa cgtttgacac
     4621 gccgctctta ctcatagggg acaaccgcgc cggctgtggc gcggcggtcc actcgggaga
     4681 gtcgggtgcg agacatcgtc gagcaggcga tgcgcggcga tcgcgaggcc ttcggatggc
     4741 tggtcggcca gaccagtgac cggatgtacg cgatcgcgac gcggatcctg cgcgacagcg
     4801 acctcgcgga ggacgccctg caaggcgccc tcatcaacgc ctggcgccag ctcccgacgc
     4861 tccgggaccc ggatcgcttc gaagcctggg tccgcaagct gctcgtccac gcctgctacg
     4921 ccgaggccag gcgtcgccgg acgtggacag cgaacgtacg ggttctgccg gtcgacgggc
     4981 cggcggctcc cgacgcatcg atctccgtcg tcgaccgcga cgcgctcgac cgggcgttcc
     5041 gacgcctcac cgtcgagcag cgtgcggtct tcgtgctgca ccaccacctc gggctgcccc
     5101 tcgtcgagat cgccgagacg ctcggtgtcc ccgccgggac cgcccgctcg agattgcatt
     5161 acgcgaccag ggcgctgcgc gcagccatcg aggctgacgc cgcgccggtc gttcccgaag
     5221 gacggatggc atgaccaagg atcgcgactt cgaccggatc gctcgagcct ggctcgacct
     5281 gatgcccgac gaagccccgg accgaacggt cgccgccgtc ctccacgccg tcgacgcgac
     5341 gccgcaggtg cggcggacgt ggggacggct gacttggagg tcaatcgcca tgaaccgcac
     5401 ggccttcgtg ctcggcgcgg cggcgatcgt cgtcgcagcc ggcgcgctgg tgatgctcag
     5461 gaccggaccg acgtcgaacg tcggttcgcc gccgacgccg agcgcgacga tcccgccgag
     5521 cccatcgccg gccggcgccg ccgccccggt gccgggcgag ctgcaggcaa tctggatggg
     5581 cgaacatcgc gaccttgtcg cgccagacgc aggcagcttg atcgagttcc gcgagaacac
     5641 gttcgcgcac gcgcaatccg ccggctcaag cgtgcaactg ctgcagtcga ccgccgcggc
     5701 gacgggtgac cgccagcttc ggctcgtgtc cgccgccgcc ggcgacgggt gcgagcaggg
     5761 ggacatcggg acctactcgt ggtccctgaa cccaagtggc cggaacttga cggtgacgca
     5821 cgagctcgac gcctgccagg cgcgggccga tgccatcgcc ggcgattggt ggctgatggg
     5881 ctgcacgacg cccgatgaca actgtctcgg cgccctcgac gcggggacct tcagctcgca
     5941 gttcatcacg ccacggctca agccgggtga gagctggagt cccgtcttcg gagcactcac
     6001 ctacacggtc cccgatgggt gggcaaacgc ctccgactgg ccagagagct tcgagctcgt
     6061 gccggcgagc gagctgccgc caatcgacga ggccaaccgg cgtcggcaca tcaatctcct
     6121 cacgcgacca agcgccatga cccaggacaa gccgtgctcc gacatggtcg agcctggcgt
     6181 cggtcgcacg atcgacgaca tcgtcacctg gctcggcacg gtgccagggc tgatcacgac
     6241 cgcgccgacg gcgatcacga ttgacggtca tccgggtcaa tggttggatg tccggctcga
     6301 cccggcgtgg gacaagacgt gcgacggcgg gtccggaccg atggtcgcgt acctgaatcc
     6361 cgggatcgcc atcagtagcg atgagcgtga acgggtactg ctcgtcgacc tcggcgacga
     6421 cgacgtcatc gcgatcgccg tgtgggcccg cgacaaggcg gcattcgact ccttcattcc
     6481 ggaggcgatg acggtcatcg agtcgttcac gttcaagtag gccgccaggt ccggatccgc
     6541 ggccacaagt gacacggtcc gcgggcggca ttgcgtggcg ctcgaacgga ccccgaccac
     6601 gcccgcgaca aggggtgggc cccgcgaatc gcgcggagcc catcgcggcg ccgtcccaac
     6661 ggctgaggtg aatttccaac gctgagcctg tcccggccac ctatggtgtc ggacggcgtc
     6721 cccgatggac gcccgtaacg gtggtgacgg tggaccgcga cctcgtcgag gcggcgcgac
     6781 acggcgacca gggagccttc atggacctgg tccgctcgcg tggtggccgc ttgttcgcga
     6841 tcgcacaccg gatcctccgg gatgtcgacc tggccgagga tgccctgcag gacgcgctgg
     6901 tgatcgcctg gcgtgacctc ccgagcctcc gcgatcccga ccggttcgac ccgtgggtcc
     6961 accgcctcct caccaacgtc tgcgtttccg aggcgacgcg cgagcgccgc cggacggcga
     7021 acctgcgggt tctcccgctc gacgggccgt cggcaccgga cgacctcatc ggggtggtca
     7081 cccgtgacca gctcgaccag gggttccggc ggctgacacc cgacgagcgc gccgtcctcg
     7141 tcctgcatca ctacgtcggc tacgccccga cggagatcgc cgagatgctc ggcgttccgc
     7201 cggggacgat ccgatcccga atccatcacg cccatcgcgc catgcgcgct gctctcgatg
     7261 ctgaggcacg caatgcgaag gccggaggtc ggacggcatg accgagcaca ccgacatcga
     7321 ccgcctcctc gagacctggt tcgacgacgg ccccacggcg atgcccgacc gggtggtcac
     7381 cgtggtcgcc gatcgcatcg gccgccaggg gcagcgacac acatggctcc tcgatcggag
     7441 gcccaaggtg aacctgttcc tcaagctcgc cgtcgccgcg gccctcgggg ccgccgtgct
     7501 cggcggtgcc atcttcatcg ccggcaggca gacccagacc ccgaccccga cgcccatccc
     7561 ggcgccgtcg tcctcgcccg gcgcatcggc cgcggcggcc ggctcacccg tcgacgacgc
     7621 cttgcgcgcg acctggaagg cgttcgccgg cgagaatccg gtcctgcaga ccggcgcggg
     7681 accggtctcg ctccagttca accccctcgg cacgggcatc gaagcgacca acttcggccc
     7741 cggccatggc tatgcgagca ccgccaacga gaccggcgct gatcgcatcg aggtcgttct
     7801 cgaccgatcg ggtggcggct gcacggccgg cgcccgcggc gtgtaccgaa cgctgctctc
     7861 ggacgatcga tccgagctca cgctgatctc ggagtccgag gactgttcga accgcggcat
     7921 cgtcctcggt cgcgcctggg cccgctcgct cgcggattcg agcctcgtgg gagccggtgt
     7981 cgtcgacgcg atcgcgcccg ccttcaaggt cgcgcttccg gacgactcgt acgagacgcg
     8041 gacgctcccc gacttcgtcg agatcggcgg cacgaacggg tcgctgatgg tgttcaagaa
     8101 cccccagccc ttcgtcgacg cctgtagcac tgacgaggag cgtgttccgt ggcagccggg
     8161 cgcggccgcg ttcatcgacg ccttccgggc caacgacgcg ttcgaggtca gcgagagcac
     8221 gccgctcacg atctctggtc acgacgccgt gcacgtccag atcggtggca aggcggacta
     8281 cgcccgctgc cccgggcagg agttgtacga gtacacgccg aaggactgcg agtgccactt
     8341 cgtcgtgggc cagggattcg ccgacagcat gtatctggtc gacgtcgacg gcgacacgtt
     8401 catgttcatc atctcgccgc tgggcacgcc cgaatccgag tggccgatcg tcgagtccat
     8461 cgagatcccc tacgagatcc catcgcgctg acggcgctga cggagcggcg ctggcgcctt
     8521 ccagcgccgc tctaccctgc tcgaaaggag cctcccatgg ttcgccgtgc ccgccgcctc
     8581 ctcgcggccg gtgccatcct cgtcgcgctt gtcatgacca ctgcggggcc cgcgtcggcc
     8641 acgctacccg gccccaacgg ccggatcacg ttcatgcggt tcgacgccga cgggcagttc
     8701 caggtctggg tcgccaaccc cgacatgagc acccaggtgc agctgacctc cggacccagc
     8761 gacgcctggt tcccggcctg gtcgccggac ggccggcgaa tcgcgttcgc cagccaccgg
     8821 accgaccccg atccgaccga cgacgtcgag atgatggacg tgttcacgat gcgcccggac
     8881 ggctcggatg tccgaaaggt caccgactcg cgcgggttca gcggtacgcc gtcctggtca
     8941 cccgatggcc ggtggctggt gttctccgcg gaccgcgccg actacccgcg gggccagggg
     9001 atctacatca tgcgcagcga cggctcgtcc gcgccgcgcc gcatcaccac gctgccgtcc
     9061 ggatcagtct ggcaggagct ggcccgattc tcgcccgacg gttcgcgcat cgtgttcagc
     9121 gagtaccgaa acgtgtcggt gcctggcgac gacggggaga gcgtcgacgt cgagcagagc
     9181 gcgctcctga ccgtgcggcc agatggcacc cacctgcgca ggatcacccc atgggagctc
     9241 agcgccagcg acggcgactg gtcccccgat gggcgccgga tcgtcttcgg cacgaggccc
     9301 aggtgggccg gcggtctcca ggaggtgatg atcgcggacg ccgacggcgg gcacctgcgg
     9361 aacctcagcc gcgaccatcc cgtgtttggt ccgccgttgc cggaggacga ctaccgcgag
     9421 tcgttcaacc ccgcgtggtc gccagacggc cgcctggtcg tgttcgtgca cgcgaaatgg
     9481 aacccgaccg acggcttcag gatgggcctc cagaccatcc ggcccgacgg ctcgcaccgg
     9541 ggctggctgt cccagggcga ggagcaccag ccggactggg ggagcgcgcg accgatccga
     9601 taggcccgat cgcactccct tcgacgccga gcccgctcct ccgggctcgg cgtccgactg
     9661 tccggcgtcc tcggtgctcg gcgcctggcc agcgcgctgc gggcgcgccg cgcacgaggt
     9721 ctctgggaca cttcgggcct gtcgtcgcat ctatggggca tggtgagcga gctttcgacc
     9781 acgggcccgg tccccgcgtc gaccgtcgaa gcggccgtcg cgggtgacgc ggtggcgttc
     9841 gcccggatcg tgcgcgccca ccacgacgac atggcccggg tctgtcgcat cgtgtgcggt
     9901 gatccggatc tcgcccagga cgcggtgcag tccgcctggc cgatcgtctg gcggaagctg
     9961 cgcacgcttc gcgagccgga gcggctgcgg ccctggctgg tcagcgtcgc ggtcaacgag
    10021 gcgcgccagc tccttcgccg cgagcaccgc gaccgagtca cggcaatcga ggtggcagac
    10081 gtgggctcgg acaggagcga tcccgcggca cgttcggccg accaggacct gctcgccgcc
    10141 gtgcgctccc tcccggccga cgagcggacg ctgctggcac tccggtatgt cgcggggttc
    10201 gatgccaccg agatcggcag tgccatgggg atgtcggcgt cgggcgttcg atcgcggctc
    10261 tcgcggttga tcgagcgatt gcgaacggag atcggcgatg actgaccggt tcgaggcccg
    10321 actcagccga ctcctgctcg tcgaggccga ggatgtcatc cgcccgatcg acgctggtgc
    10381 gatggcagcg gcggcgatgg gcgaggtccc aatgcgccgc ggcatgccca tggtcgggac
    10441 ccgcgcggcg cgggcgctcg tgctcgcggg gctgcttacg ctcgggctgc tcgcgaccat
    10501 cgccctgctc gccggctcgc agcgtgggcc ccgaccgttc gccggcgtgc tcgacccgac
    10561 cggtccgatg accgacggga tgctgcagcc ggcgaccctg gcacgacccg acggcacggt
    10621 gctcgtcgcg ggagccacgg cggacatgcg gctgcacatg gagtcgttcg acgctgcgac
    10681 cgggcggttc acggtcctgc cgggcctcga cgaccgacgc ttctcgcacg gacgcgacga
    10741 gctcgggctc gtcccgctcg ccgatggcac ggtgctcgtg gtcatggcct ccgcgtccag
    10801 ccagagcggc gcgaccgagg gcggctacgc gctgcgctac gacctcgcaa cccgcgaagt
    10861 gcgcgagacc gccggcacct tcgcgcaaac cgacccgcgc cgcctggtcg gcgaattccg
    10921 gttcgccagt tggccctgga gccggcgtgc cgacggcacg atcgcgtgga cgccacccac
    10981 ttggatcatg agcgacgtgc ccgtctacga ccctcgtttg aacgcgttct ccgtcgagcc
    11041 cgggccggcc tcgccgttca cgtcgatccc gcccgaagct gacccctttg cgaccgacct
    11101 gtacctcggc ccgagtgcga tcgtccacct gcgcgatgga cgcctcctgc tcctgggtgc
    11161 cagccgcgac tccaacgcgc cggcggccat ggtggacggc gctacgttcg ccggccaccc
    11221 gctggactgg ccgctcgtcc gcggcggcac ctatgcgggc gtcgtgcttc ccgatggccg
    11281 ggtgctcatc gccggcccca cctcgcacgt cttcgacccc tccacggaca agctggtcga
    11341 cctcgacgtc gtcgacgccc tctcgccggc ggttcagctc cgcgacgggc gcgtcctgct
    11401 ggtcggtgag gcgccgacgt cgcttgggtc gcccgcccgc taccggttgt ggcaattcga
    11461 tcctgcgacg acgacgctgg agcgcgttgg acgtggcgac gccccggctc cgacgtcggg
    11521 ctggacggag ttgagcgacg gccaggtgct cgtcgtaagc ggctacgccg gcacaggcga
    11581 cgcgctgccg cgggacgcct ggatcctccg ctagccaagg cggactcggt cggccggtct
    11641 gacaggcaag cgaaccgggc tgaacgcgtc ccgatggccg gatcgtggct gtcaacgacc
    11701 ggaatctggc ctgggggacc ggatcggcgg cgctggcggt tcgcagaccg gccttcgtcc
    11761 cgaatcgctg gcctggcagg ccagcgggcc gaccgcgaga accgctgatc gattcgactc
    11821 cggggggtcg aaccgatagc attggaaaaa cccccggagg acctgacccg tggcgaagta
    11881 tgtctttgtg accggaggag tgacttcctc actcggcaag ggcatcaccg ccgccagcat
    11941 cggacgcctg ctcaaggcac ggggtctcaa ggtctcggtc ctcaagctcg acccctacat
    12001 caacgtcgac ccggggacga tgtcgcccta ccagcacggc gaggtgttcg tcaccgacga
    12061 cggcgccgag accgacctcg acctgggcca ctacgagcgg ttcatcgacg agaacctgtc
    12121 ccagagctcc aacgtcacga ccggccgcat ctaccaggcc gtgatcgcga aggagcgccg
    12181 cggcgactac ctcggtggca cggtccaggt catcccgcac atcaccaacg agatcaagga
    12241 gcggatcacg cgcgtcgcgc gcgccggcga tccggacgtg gtcatcgtcg aggtcggcgg
    12301 gacggtcggc gacatcgaga gcctgccgtt cctcgaggcc atccgccaga tgcgcaagga
    12361 ccagggccgc gcgaacgtgc tgtacgtgca cgtgacgctg ctgcccgcgc ttgccgcaac
    12421 cggcgagctc aagaccaagc cgacgcagca ctccgtcaag gagctgcgcg ggatcggcat
    12481 ccagcccgat gtcatcgtcc tgcgctcgga ctccgaggtt cccgacgaga tccgcgagaa
    12541 gatcgcgctg ttcaccgatg tcgacgtcga tgcggtcatc cccgcggcca ccgccgagac
    12601 gatctacgag gtgccgctcc agttcgaggc cttcggcttc gggcggctcc tggtccgcga
    12661 gctgggcctg ggtgacgccg acgtggtgcc ggatctcgac ggctggcggg cgctggtcga
    12721 acgaatcaag gcccccaagg cgacgctcga gatcgcgctc gtcggcaagt acatcgagct
    12781 gcccgacgcc tacctctcgg tcaccgagtc gctcaagcac gcagcctggg cgagcggcgt
    12841 ggatgccagg gtgcgctggg tcgacagcga gcagctgacg gcggacaacg tcgccgagcg
    12901 gctcgacggc gcggcgggcg tgctcgtgcc cggcggcttc ggccaccgcg ggatcgaggg
    12961 caaggtcctc gccgctcact gggcgcgcga gcgcggcgtg ccctatctcg gcctgtgcct
    13021 cggcctccag tgcgcggtga tcgagttcgc gcgcgagatc atcggcaccg gcgacgtcaa
    13081 ctcgaccgag ttcgacatgt tcacgaccaa ccccgtcatc gacttcatgc ccgaccagcg
    13141 cgacctcgag gacaagggcg gcacgatgcg cctcggcctc tacccggcca agctgctgcc
    13201 ggggtcgaag gcccgcgccg tctacggcca ggaggtcgtg tacgagcggc atcgccaccg
    13261 attcgaggtc aacaaccgct accgccaggt cctcgagggc gcgggcatgc tgctgtccgg
    13321 ggtgtcgccc gacggccgcc tggtcgagat cgtcgagctg cgcgaccacc cgtggttcgt
    13381 ggccagccag ttccacccgg agttcaagag ccggccggag cgcccgcacc cgctgttcga
    13441 cgggttcgtc accgcggcga tcgccgtggg cgagggtcgg gagccggtct tccacgcgcg
    13501 cgaaccgcag atcgagcagg ggcgcgagcc ggacaccggc gcgacgtcgg cggatgctat
    13561 cgagtccatc cgcggcgcct gaggtcgctg gccgaccctc cctggccgga cgtgccgccg
    13621 gggggttcgg cgcgtcgatc ctggcggggg tccttctcgg cggcagtgcc tggttctccg
    13681 atcagctcgc ctggccctac agcct
//
LOCUS       DBNHXO010000196         4521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1442910, whole genome shotgun sequence.
ACCESSION   DBNHXO010000196 DBNHXO010000000
VERSION     DBNHXO010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1442910"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1088
                     /locus_tag="AC162A_04215"
     CDS             84..1088
                     /locus_tag="AC162A_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5010076.1"
                     /translation="MSAAAGIVPETRPAPGELIAAWARRNAWTLGLIAVLIGMLVFTK
                     LIQPNYGAPQIQGLAIGMLPVALAAIAQAIVVISGGIDLSVASMMALTSVTAAVLMEG
                     QGEGFAVLVVLAILLLGLAVGAINGSLIVVTRVPDIVVTLAMAFVWAGATLLVLDSPG
                     GGAAEWVKGLVNGSLGSPWVPRALLVLVAIVVVIWIPLRRSMLGLSMYALGSSQLAAF
                     RSGVSVGRTKIAAYAITGLFCALGGLAVIASTGSGSPVPGPYTLQSVAAIVLGGVSLA
                     GGRGGVVGPIVAVAILQLVRTDLTLLDVNPNLATAVQGVILISVVLVGSVITMRRNRP
                     "
     gene            1133..2074
                     /locus_tag="AC162A_04220"
     CDS             1133..2074
                     /locus_tag="AC162A_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5010077.1"
                     /translation="MRRLFRDRPLIPLTALLVLLVVVLELVQPGTVSPNWVAIALRAA
                     IPLAILAGCQTLAMLTGGIDLSVGTIASMAAFLVATQSTSQGPVVAILIGLGAAVLAG
                     TINGIGIGVFKVHPLIMTLGIGLVILGLMAAYQLAMVTSGVELPDSIQWLGSGLDIGF
                     LPNSLLLFVPVAAAILLGLRYTGFGRLLFAVGDNPVASRLSGVRVWQVLVALYILSAV
                     LAALAGLLVAGLVKTASISLVESSVLPSVAAAVIGGTSIMGGRGGYAGTIVGALILTV
                     LSALLGALDFPEAVRQILFGLIVVVVAAAYARVTGEA"
     gene            2083..3030
                     /locus_tag="AC162A_04225"
     CDS             2083..3030
                     /locus_tag="AC162A_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR5010078.1"
                     /translation="MDDGGPGTRHLGLDLGGTNLKWAVVARDGDSWTTLATGQGPTEA
                     GEGPDAVVARMGEVGAAVCNDWPGVVSAGLGVPGLYDPEAGTTRFLVNVPGEWDGRPV
                     AGPVGERLGVPTSLINDARAFGLAELRLGAGRGADSMVGLTLGTGVGGVIAIDGKVLQ
                     GHDGTAGEVGHQTIDPDGPWCGCGNRGCLEAFARADQIAAACGTSTAEQAVARAREGD
                     ARALDGLAMVGRYLGIGIANMVTVIAPDRVVIGGGIAAAGELLLAPIRAELARRVKTT
                     SLEPVEVVLAELGTWAGSIGAAVHGAERAAAAREQAARR"
     gene            3033..3770
                     /locus_tag="AC162A_04230"
     CDS             3033..3770
                     /locus_tag="AC162A_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR5010079.1"
                     /translation="MAATVPHWRRIELALRERIHELAPGDSLPSDTELCDEFGVSRMT
                     ARNAMQRLADEGLVVRVPGRGSFVAEAPAHRRADRLMTFSHEMERRGRLPSSRVLARE
                     IRPSTADEAGDLGIVPSEPVVVVRRVRCADGEPIAVETARLVGRTAPSVMTADLEHGS
                     LHEALARGGHDLRRGLATITAAAATHEDARLLGIRRGDPLLVERRVISDGRGRRVEAT
                     ESRYPADRYALDVQFDVEDTRSDASEP"
     gene            3767..>4521
                     /locus_tag="AC162A_04235"
     CDS             3767..>4521
                     /locus_tag="AC162A_04235"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046349 - amino sugar biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR5010080.1"
                     /translation="MTAELVAGRLVLPDAVVPGRITIEGGWITAVEPDDAAAAGPYLA
                     PGFVDVHVHGWGGHDATGDADALSGMARALLRRGVTSFLPTAPSLPEAELPRFADRVR
                     AWIPAAPDDGAQPLGFNLEGPFLAPSRRGAHDPAALRTPAQLGADAVTPLLDGLRVMT
                     IAPELPGALELIGRLSALGIAASMGHSASTLDEARAGYLAGGRTTTHLFNAMTGLDHR
                     APGLAVAALIDDDAYVELIADGHHVHPAVWPIIT"
BASE COUNT          516 a         1636 c         1699 g          670 t
ORIGIN      
        1 acgatgcccg aggaggtcgc ggcgtcggag ctcgccgcgg acagcgcggg tgatgcgccg
       61 gcccagccga gtgaggtggt gacgtgagcg cggccgcggg catcgtcccc gagacccgcc
      121 ccgcccctgg ggagctcatc gctgcatggg cgcgccgcaa cgcgtggacc ctcgggctga
      181 tcgcggtcct gatcggcatg ctcgtgttca ccaagctcat ccagccgaac tacggtgcac
      241 cgcagattca gggcctggcc atcggcatgc tgccggtcgc gctggccgcg atcgcccagg
      301 cgatcgtcgt gatctcgggt ggtatcgatc tctcggtcgc gtcgatgatg gcgctcacca
      361 gcgtcaccgc ggccgtgctg atggaaggcc agggcgaggg cttcgcggtc ctggtggtgc
      421 tggcgatctt gctgctcggc ctggccgtgg gagcgatcaa cggctcgttg atcgtcgtca
      481 cccgcgtccc agacatcgtg gtcacgctgg cgatggcgtt cgtctgggcc ggggcgacgc
      541 tgctcgttct cgactcgccc ggcggcggcg cggcggagtg ggtcaagggc ctcgtgaacg
      601 gctccctcgg cagtccgtgg gtgccgcggg cactcctcgt gctcgtcgcc atcgtcgtcg
      661 tgatctggat cccgctccgg cggtcgatgc ttggcctcag catgtacgcg ctcggcagca
      721 gccagctggc agcgttccgg agcggcgtgt cggtcggccg gacgaagatc gccgcctacg
      781 cgatcaccgg gctgttctgt gcgctcggcg gcctggccgt gatcgcgagc acgggttcag
      841 ggtcgccggt gcccgggccg tacacgctcc agagcgtcgc cgcgatcgtg ctcggcgggg
      901 tcagccttgc cggcggccgc ggcggcgtcg tcggccccat cgtcgcggtg gcgatcctgc
      961 agctcgtccg caccgacctg acgctgctcg acgtcaaccc caacctcgcc acggccgtcc
     1021 agggggtcat cctgatcagc gtcgtcctgg tcggcagcgt catcaccatg cggcggaaca
     1081 ggccatgacg gcgggagtcg ccgcggcccc ggctcccagc gccggatcgg cggtgcgccg
     1141 cctgttccgg gatcggccgc tgatccccct gacggcgttg ctcgtgctgc tcgtcgtcgt
     1201 cctggagctc gtccagcccg gcacggtgag tcccaactgg gtggccatcg cgctccgggc
     1261 ggcgatcccc ctcgcgatcc tggccggctg ccagacgctc gcgatgctca ccggcggcat
     1321 cgacctctcg gtcggcacga tcgcctcgat ggcggcgttc ctggtcgcga cgcagtcgac
     1381 gagccagggt ccggtggtcg cgatcctcat cggcctcggc gccgcggtcc tcgccgggac
     1441 catcaacggg atcgggatcg gcgtgttcaa ggtccacccg ctgatcatga ccctcggcat
     1501 cgggctcgtg atcctgggcc tgatggccgc ctaccagctg gcgatggtga cctcgggcgt
     1561 ggagctcccg gactcgatcc agtggctcgg ctcgggcctc gacatcggct tcctgcccaa
     1621 cagcctgctg ctgttcgtgc cggtggccgc ggcgatcctg ctcggcctgc ggtacacggg
     1681 gttcggccgg ctgctgttcg ccgtcggcga caaccctgtg gcctcgcgcc tgtcgggggt
     1741 gcgggtgtgg caggtgctcg tggcgctgta catcctgtcg gcggtgctgg cggcgctcgc
     1801 cgggctcctc gtcgccgggc tggtcaagac ggcgagcatc tcgctcgtgg agtcctcggt
     1861 actgccgtcg gtcgcggccg cggtcatcgg cgggacgtcg atcatgggcg ggcgcggggg
     1921 gtatgcgggg acgatcgtcg gggcactgat cctgaccgtg ctctcggcgc tgctgggcgc
     1981 gctggacttc cccgaggccg tgcgccagat cctgttcggc ctgatcgtcg tcgtcgtcgc
     2041 ggcggcctac gcccgggtca ccggcgaggc ctgacggccg cgatggacga cggcggcccc
     2101 ggcacccggc acctcggcct cgatctcggc ggcaccaatc tcaagtgggc ggtggtcgcc
     2161 cgcgacggcg attcctggac cacgctggcg accggccagg gcccgaccga ggccggcgag
     2221 ggacccgacg cggtcgtcgc ccgcatgggc gaggtcgggg cggcggtctg caacgactgg
     2281 cccggcgtgg tgtccgccgg gctgggcgtg cccggcctgt acgaccccga ggcgggcacg
     2341 acgcggttcc tggtcaacgt gccgggggag tgggatggcc ggccggtggc cggcccggta
     2401 ggggagcgac tcggcgtgcc cacgtccctg atcaacgacg cgagggcctt cgggctcgcg
     2461 gagctgcggc tgggcgccgg ccggggtgcc gactcgatgg tcggcctcac gctgggcacg
     2521 ggtgtcggcg gcgtgatcgc gatcgacggc aaggtcctcc agggccacga cggcacggcc
     2581 ggggaggtcg gccaccagac gatcgatccg gacgggccct ggtgcggctg cggcaaccgc
     2641 ggctgcctcg aggccttcgc gcgcgccgac cagatcgcgg ccgcctgcgg gacctcgacc
     2701 gcggagcagg cggtggctcg cgcccgcgag ggtgacgccc gggcgctcga cgggctggcc
     2761 atggtcggcc gctacctggg catcgggatt gccaacatgg tcaccgtgat cgccccggac
     2821 cgggtcgtga tcggaggcgg catcgcggcc gcgggcgagc tgctgctcgc gcccatccga
     2881 gcggagctgg cgcggcgcgt gaagacgacg tcgctcgagc ccgttgaagt cgtcctcgcc
     2941 gagctcggca cgtgggcggg ctccatcggg gcggccgttc acggggccga acgcgccgcc
     3001 gcggcgcgcg aacaggccgc gcgacgatga ccatggcggc aaccgtcccg cactggcgcc
     3061 ggatcgagct ggcgctgcgg gaacgcatcc acgagctggc gcccggcgat tcgctcccct
     3121 cggacaccga gctgtgcgac gagttcggcg tcagccggat gacggcgcgc aacgcgatgc
     3181 agcggctcgc cgacgaaggc ctggtcgttc gggtcccggg gcgtggcagc ttcgtcgccg
     3241 aggcgccagc gcaccgtcgc gccgaccggt tgatgacgtt ctcgcacgag atggagcgcc
     3301 ggggccgcct gccgtcgtcg cgcgtgctcg cgcgcgagat ccggccgtcg accgcggacg
     3361 aggccggaga cctcggcatc gtcccgtccg agcccgtggt cgtcgtgcgc cgggtgcgct
     3421 gcgctgacgg cgagccgatc gccgtcgaga cggcgcggct cgtgggccgc accgcgccga
     3481 gcgtgatgac ggccgatctc gagcatggct cgctccacga ggcgctggcg cggggcggcc
     3541 acgacctgcg gcgcgggctg gcgaccatca ccgccgcggc ggccacccac gaggatgcgc
     3601 gcctgctcgg catccggcgt ggcgacccgc tgctcgtgga gcggcgcgtg atctccgacg
     3661 gccgcggacg acgcgtcgag gccaccgaga gccgctatcc cgccgatcgc tacgcgctcg
     3721 acgtgcagtt cgacgtcgag gacacgcgat cggacgcgag cgagccgtga ccgccgagct
     3781 cgtcgcgggg cggctcgtgc tgcccgacgc cgtcgttccc ggccggatca cgatcgaggg
     3841 cggatggatc accgcggtcg aaccggacga tgcggcggcc gccggcccct acctcgcccc
     3901 ggggttcgtc gacgtccatg tccacgggtg gggcggccac gacgcgaccg gcgacgcgga
     3961 cgccctctcg gggatggccc gcgcgctgtt gcggcgcggc gtgacgtcgt tcctgcccac
     4021 ggcaccgtcc ctccccgagg cggagctgcc gcggttcgcc gaccgcgtcc gggcctggat
     4081 cccggcggcg cccgacgacg gcgcgcagcc cctggggttc aacctcgagg gccccttcct
     4141 ggcgccctcc cggagaggcg cccatgaccc ggcggccctg cgcaccccgg cccagctggg
     4201 cgcggatgcg gtcaccccgc tcctcgacgg cctgcgggtc atgaccatcg cgcccgagct
     4261 cccgggggcg ctcgagctca tcgggcgcct gtccgcgctg ggtatcgcgg cgtcgatggg
     4321 ccactcggcg tcgacgctcg acgaggcgcg cgcgggctac ctggccggcg gtcgcacgac
     4381 gacccatctg ttcaacgcca tgacgggcct cgaccaccgc gcgcccgggc tggcggtcgc
     4441 cgccctcatc gacgacgacg cctacgtgga gctcatcgcc gacggccacc acgtgcaccc
     4501 ggccgtgtgg ccgatcatca c
//
LOCUS       DBNHXO010000197         2695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3824379, whole genome shotgun sequence.
ACCESSION   DBNHXO010000197 DBNHXO010000000
VERSION     DBNHXO010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3824379"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="AC162A_04240"
     CDS             <1..189
                     /locus_tag="AC162A_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HIR5010081.1"
                     /translation="PSRAVCVPSSEPNRLLGRLRAGSPAVVGRIERDSVVLDLRSVEP
                     ADDAPLAAAVRSALDGHG"
     gene            182..2059
                     /gene="selB"
                     /locus_tag="AC162A_04245"
     CDS             182..2059
                     /gene="selB"
                     /locus_tag="AC162A_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HIR5010082.1"
                     /translation="MADERPAPSVVIGTAGHIDHGKTTLLHALTGIDADRLPEERRRG
                     MTIDVGYAHLTLDDGSTVDFVDVPGHDRLVGNMLVGAGEIDAALLVVAADDGPNAQTL
                     EHLGLLDALGIRDGLVVVTKADLADEARVAVVAAEVGALLAATTLAGAPVVGASATTG
                     DGIETVRNATATIARRVQGLLGDGRAPRLAIDRSFTVMGRGTVVTGSLRGGPIRAGMT
                     MRLVPGDDQVRVREVQVRGETVDEAAGGRTALLIGFDGEPPRRGQVLSTDRDVVATSR
                     LLVALRGPTAAAAPADRERLRVHLGTEQAGVLVVRGPREAIDLPDGTSIAILRLDATI
                     AAAPGDRFALRRPSPGSVAGGGIVLDATPPRGVSRRRLTPDRAAALAAGPVGSLDARV
                     ELHGAILVDGTWRLAPDVEAALGDRAVALVTAHHEAQPDSAGLPAASLRADLGPASRR
                     HATLTRDAAVTVAGAVVDRLVADGTLARDEDRIRTPDRAAGLSPATREAMDRLEAGLA
                     VATPPPLAEAARAADCPPDGVRALEATGRIVRLEDDLAWAAPTYRDLVRRALSMAAAG
                     PLTPAAYRDATRSSRRYVMVILEDMDRRGLLQRTDAGHVLGPTTLARMRERASTAARG
                     Q"
     gene            2056..2667
                     /gene="mobA"
                     /locus_tag="AC162A_04250"
     CDS             2056..2667
                     /gene="mobA"
                     /locus_tag="AC162A_04250"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HIR5010083.1"
                     /translation="MTAAIVLAGGASTRFGSDKLLADLDGRPLLDHALEAVSAIAGPV
                     ILVIAPDAPAPPIPAGLTRQVSLMRDDIAHGGPLAGLAAGLAALADIDRGFDGPAIVV
                     GGDMPYLVPAVLALLAATVDADPAFGGASLEADPPCVLPLAIRPSLARPVIDALLLED
                     RRALRALLDRLPMALVAAGDWRSLDPGGRTLIDIDTVADLRAG"
BASE COUNT          305 a         1050 c          967 g          373 t
ORIGIN      
        1 ccgtcgcggg ccgtgtgcgt gccgtcgtcc gaacccaacc ggctgctggg ccggctgcgg
       61 gccggttcgc cggcagtcgt cgggcggatc gaacgagact cggtggtgct tgacctgcga
      121 tccgtcgagc cggccgacga tgcgcctctc gccgcggcgg tgcgttctgc cctcgacggc
      181 catggctgac gagcgcccgg cgccgagcgt cgtcatcggc acggccggcc acatcgacca
      241 tggcaagacg acgctcctgc atgcgctcac cggcatcgat gccgaccgcc tcccggagga
      301 gcgccggcgg ggcatgacga tcgacgtcgg ctacgcccat ctgacactgg acgatgggtc
      361 gacggtcgat ttcgtcgacg tgccggggca cgaccgcctg gtcggcaaca tgctggtcgg
      421 ggccggcgag atcgacgcgg cgctgctggt cgtggccgcc gacgacgggc cgaacgccca
      481 gacgctcgag catctcgggc tgctcgacgc gctcgggatc cgtgatggcc tcgtggtcgt
      541 gacgaaggcc gacctggccg acgaggcgcg ggtcgccgtc gtcgccgccg aggtcggagc
      601 gctgcttgca gcgacgacgc tcgccggggc tccggtcgtg ggggcgtcgg cgacgaccgg
      661 tgacggcatc gagaccgtac gcaacgcgac cgcgacgatc gcgcgccgag tgcaggggtt
      721 gctcggcgat ggacgggccc cacggctggc gatcgaccgc agcttcacgg tcatgggccg
      781 gggcaccgtc gtgaccggga gcctgagagg cgggccgatc cgcgccggga tgacgatgcg
      841 gttggtgccc ggcgacgatc aggtccgcgt tcgggaggtc caggttcgcg gcgagacggt
      901 cgacgaagct gcgggcggac ggacggcgtt gctcatcgga tttgacggcg agccaccgcg
      961 gcggggccag gtgctgtcga cggatcgcga cgtcgtcgcc acgtcgcggc tcctcgtcgc
     1021 tctgcgtggt cccaccgcgg cagccgctcc ggctgaccgg gagcgtcttc gcgtgcacct
     1081 cggcaccgaa caggcgggtg tcctcgtggt gcgcgggccg cgcgaggcga tcgacctgcc
     1141 cgacgggacc tcgatcgcca tcctccgcct cgacgcgacg atcgccgcgg ctccaggcga
     1201 ccgcttcgcc ctgcgccggc cgtccccggg gtccgtcgcg ggcggcggca tcgtgctcga
     1261 cgccactccg ccgcgtggcg tgtcgcgccg acggctgacg ccggaccgtg ccgccgccct
     1321 ggccgccggg ccagtcgggt ccctggacgc ccgcgtcgag ctgcacggcg cgatcctcgt
     1381 cgacgggacg tggcgcctgg cgcccgacgt cgaggcggcg ctcggcgatc gagccgtcgc
     1441 ccttgtcacg gcccaccacg aggcccaacc cgactcggcc ggtctcccgg cggcgtcgct
     1501 ccgcgccgac ctcggcccgg cctcccgtcg ccacgcaacg ctgacgcgcg acgcggctgt
     1561 caccgtcgcg ggcgcggtgg tcgatcgcct ggtcgccgac gggaccctcg cccgcgacga
     1621 agaccggatc aggacgccgg accgcgcggc gggcctctcc cctgcgacgc gcgaggcgat
     1681 ggaccggctc gaggcagggc tggccgtcgc gacaccgccc ccgcttgccg aggccgctcg
     1741 ggccgccgac tgcccgcccg atggcgtccg ggccctcgag gccaccggcc ggatcgtccg
     1801 cctcgaggac gaccttgcct gggccgcgcc gacctaccgg gatctcgtcc gccgcgcgct
     1861 gtcgatggcc gccgccgggc cgctcacacc ggcggcctat cgcgatgcga cccgctcgag
     1921 ccgccgctat gtgatggtca tcctagagga catggatcgg cgcggcctgc tccaacgcac
     1981 cgacgccggc cacgtcctcg gtccaacgac cctcgcccgg atgcgggagc gggcgtcgac
     2041 cgcggcccgc ggccaatgac cgccgcgatc gtgctcgccg gcggcgcctc gacgcgcttc
     2101 ggctccgaca agctgctcgc cgatctcgac ggtcgcccgc tgctcgatca tgccctcgag
     2161 gccgtctccg ccattgctgg tcccgtgatc ctcgtcatcg cccccgacgc ccccgcgccg
     2221 ccgatccccg ccggccttac ccgccaggtg tcgctgatgc gcgacgacat cgcccacggc
     2281 ggcccgctcg ccgggctcgc tgccggcctg gccgccctcg ccgacatcga tcgtggcttc
     2341 gacggccccg cgatcgtcgt cggcggcgac atgccgtacc tcgtgcccgc ggtcctggcg
     2401 ctcctcgcgg cgaccgtcga cgccgacccg gcgttcggcg gcgcgagcct cgaggccgac
     2461 ccaccctgcg tgctgccgct ggcgatccgg ccgtcgctcg cgcgacccgt catcgacgcc
     2521 ctgctgctcg aggatcgccg ggccctgcgc gccctgctcg accgcttgcc gatggccctc
     2581 gtcgcggccg gcgactggcg ttcgctcgac ccgggcgggc gcacgctcat cgacatcgac
     2641 accgtcgccg acctccgcgc cggttgacag ccactctcat ggtcctatga tggtg
//
LOCUS       DBNHXO010000198         4064 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1640889, whole genome shotgun sequence.
ACCESSION   DBNHXO010000198 DBNHXO010000000
VERSION     DBNHXO010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1640889"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2122
                     /locus_tag="AC162A_04255"
     CDS             <1..2122
                     /locus_tag="AC162A_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020620964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR5010084.1"
                     /translation="TQKQPSVLPAKLPNLLVNGSSGIAVGMATNIPPHHLGEVVDAVV
                     AYIDNPDVTNDDLCQLIKGPDFPTGGSIYRFESRRNPLTGEHETIDAIREMYAHGHGR
                     VVMRAQVAFEETRADRTAIIVTELPYQVNKATLLEKIADLVKDKRIDGIADLRDESDR
                     DGMRIYIEMKRDANPHKVLNNLFKHTSMQTAFNLNMLALVNGQPQTLPLKSVLRHYTD
                     HRREIVRRRTEFDLEKARARAHILEGLKIALDNLDAVIETIRRSDDVDAARTNLMTRF
                     GLSEPQAQAILDMRLARLAALERKKIEDEYLAVIQQIAELEDILANPARVLSIIKDEL
                     AELKRKYAGERRTRVVEDSSREMTDEDLIANEDVVVTISRRGYIKRQPVATYRRQHRG
                     GKGIIGHVTREEDAVEHLLAANTHDWALFFTNRGRVFSAKVHMIPDASRQAKGMAIIN
                     LPGVQVETGEVPMATICLPNFEPGHYLVMATRNGIIKKTPLEQFERVRATGIRAITIS
                     DSDELAWVSVSSGEDDVIIATAQGMIARFSEGEVRPMGRDAAGVIGIRLLARKDDNVV
                     SMTVVKPDADLLVLTETGYGKRVALTEFRKKHRGGQGVRLIALEGRKTGLVAAVQQVT
                     PEDEELLLISAHGQVIRTETNTINRYSSSARGVIVMRLADEDKVVAIAAFSPGLAERG
                     GMGDNDDPQADGGGSDDPGPGRSS"
     gene            2119..3210
                     /locus_tag="AC162A_04260"
     CDS             2119..3210
                     /locus_tag="AC162A_04260"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR5010085.1"
                     /translation="MTAFGQREAALYADQYEIYHGNANPELARKIAHWLNSEPGQAEV
                     FQFANENIFVRILDNVREKDVFLVQPTCRPVNQSIMELLIMIDAFKRASAGRITAVVP
                     YYAYGRSDKKDQPRVPITARLIADMITVAGADRMLTMDLHQGQIQGFFNIPVDELTAV
                     HMLSRYFIDKHLTECVVVTDLGFAKRARAFAEILDAPLAIVEKRRHGNLDRAEVINVI
                     GDVHGRRAIIVDDEIDTAGTLIETTRALEREGVTEIYACATHGVLSDPAIERIANSSL
                     RELVITDSVPLPASKVLPQIRVLSVAPLIGEAIKRIHRGESVGALFSSEVSFTQEMLL
                     WEDGPIQPGRESPAGDGDVPSPMAASAGA"
     gene            3229..3492
                     /locus_tag="AC162A_04265"
     CDS             3229..3492
                     /locus_tag="AC162A_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010086.1"
                     /translation="MSLQLHRPDGKGGLEPRPVSDENWHSQLRSPRWGASLRGGRLPR
                     LANPEMNPTSTAKSVAFWLVLGLLTFGVIVVGYGSGFFKFVTA"
     gene            3571..3816
                     /locus_tag="AC162A_04270"
     CDS             3571..3816
                     /locus_tag="AC162A_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010087.1"
                     /translation="MQRVLIASGVLGLGTALVFGAAAVTATLFPKGPMVAAGMNEIWM
                     RNGGGVMMGVGKPMPMPAPGFAIDDGKDVIDLPGVQQ"
BASE COUNT          722 a         1445 c         1334 g          563 t
ORIGIN      
        1 cacccagaag cagccgtccg tcctgcccgc caagctgccg aacctgctcg tcaacggctc
       61 gtcggggatc gccgtcggca tggcgaccaa catcccgccc caccacctgg gcgaggtcgt
      121 ggacgccgtc gtcgcctaca tcgacaaccc cgacgtcacc aacgacgacc tgtgccagct
      181 gatcaagggt ccggactttc cgacgggcgg ctcgatctac cgcttcgagt cgcggcgcaa
      241 cccgctgacg ggcgagcacg agacgatcga cgcgattcgc gagatgtacg cccacggcca
      301 cgggcgggtt gtcatgcgcg cgcaggtcgc gttcgaggag acccgcgccg accgcacggc
      361 gatcatcgtc accgagctgc cgtaccaggt gaacaaggcg acgctgctcg agaagatcgc
      421 cgacctggtc aaggacaaga ggatcgacgg gatcgcggac ctgcgcgacg agtcggaccg
      481 cgacggcatg cgcatctaca tcgagatgaa gcgggacgcg aacccgcaca aggtgctcaa
      541 caacctgttc aagcacacct cgatgcagac ggcgttcaac ctcaacatgc tggccctggt
      601 caacgggcag ccacagacgc tgccgctcaa gagcgtcctg cgccactaca ccgaccaccg
      661 ccgagagatc gtccgccgcc ggaccgagtt cgacctcgag aaggcgcggg cccgagccca
      721 catcctcgag gggctcaaga tcgccctcga caacctcgac gcggtcatcg agacgatccg
      781 ccgctcggac gacgtcgacg cggcacgcac gaacctgatg acgcgcttcg gcctgtccga
      841 gccgcaggcc caggcgatcc tcgacatgcg cctcgcccgc ctcgcggccc tcgagcgcaa
      901 gaagatcgag gatgagtacc tcgccgtcat ccagcagatc gccgagctcg aggacatcct
      961 cgccaacccg gcccgcgtgc tgtcgatcat caaggacgag ctcgcggagc tgaagcgcaa
     1021 gtacgccggc gagcggcgca cccgcgtcgt cgaggactcc agccgcgaga tgaccgacga
     1081 ggacctgatc gccaacgagg acgtcgtcgt tacgatcagc cggcgcggct acatcaagcg
     1141 ccagccggtc gccacctacc gccgccagca tcgcggcggg aaggggatca tcggccacgt
     1201 cacccgcgag gaggatgccg tcgagcacct gctggcggcc aacacccacg actgggcgct
     1261 gttcttcacc aaccgcggcc gggtgttctc ggccaaggtc cacatgatcc ccgacgcctc
     1321 gcgccaggcg aagggcatgg cgatcatcaa cctccccggc gtgcaggtgg agacgggcga
     1381 ggtcccgatg gccaccatct gcctgcccaa cttcgagccc gggcactacc tggtcatggc
     1441 gacccgcaac gggatcatca agaagacgcc gctcgagcag ttcgagcgtg tgcgcgcgac
     1501 cgggatccgg gcgatcacga tcagcgactc cgacgagctg gcctgggtga gcgtctcgtc
     1561 cggcgaggac gacgtgatca tcgccacggc gcagggcatg atcgcgcgct tctccgaggg
     1621 agaggttcgc ccgatgggcc gcgatgcggc gggtgtcatc ggcatccgcc tgctggcccg
     1681 gaaggacgac aacgtcgtct cgatgaccgt ggtcaagccc gacgccgacc tgctggtcct
     1741 gaccgagacc ggctatggca agcgcgtggc gctcacggag ttccggaaga agcaccgcgg
     1801 cggccagggc gtccggctga tcgccctcga gggtcgcaag accggcctcg tcgcggccgt
     1861 ccagcaggtg acacccgagg acgaggagct gctgctgatc tcggcccacg gccaggtcat
     1921 ccgcaccgag accaacacga tcaaccgcta ctcgtcgagc gcgcgcggcg tgatcgtcat
     1981 gcgcctcgcg gacgaggaca aggtggtggc gatcgcggcc ttcagcccgg ggctggcgga
     2041 gcgcggtggc atgggcgaca atgacgaccc ccaggcggac ggcggcggat cggacgaccc
     2101 ggggccaggt cgctcgtcgt gaccgccttc ggccaacggg aggcagccct gtacgcggac
     2161 cagtacgaga tctaccacgg caacgccaac cccgagctgg cgcggaagat cgctcactgg
     2221 ctgaacagcg agcccggcca ggccgaggtg ttccagttcg cgaacgagaa catcttcgtg
     2281 cgcatcctgg acaacgtccg cgagaaggac gtgttcctcg tccagccgac ctgtcgcccg
     2341 gtcaaccagt cgatcatgga gctgctgatc atgatcgacg ccttcaagcg ggcgtcggcc
     2401 gggcgcatca cggccgtggt gccgtactac gcctacgggc ggtccgacaa gaaggaccag
     2461 ccgcgcgtcc cgatcacggc ccgcctgatc gccgacatga tcacggtcgc cggcgccgac
     2521 cggatgctga cgatggacct gcaccagggc cagatccagg ggttcttcaa catccccgtc
     2581 gacgagctga cggccgtcca catgctcagc cgctacttca tcgacaagca cctgaccgag
     2641 tgcgtcgtcg tgacggacct cgggttcgcg aaacgcgccc gggccttcgc ggagatcctc
     2701 gacgcaccgt tggccatcgt cgagaagcgg cgccatggca acctcgaccg cgccgaggtg
     2761 atcaacgtga tcggcgacgt ccacggacga cgggcgatca tcgtcgacga cgagatcgac
     2821 acggccggaa cgctgatcga gacgacgcgc gcgctcgagc gcgagggcgt gaccgagatc
     2881 tacgcgtgcg cgacccacgg cgtgctctcc gaccccgcca tcgagcgaat cgcgaactcg
     2941 agcctgcggg agctcgtgat caccgacagc gtcccgctgc cggcctcgaa ggtcctgccc
     3001 cagatccgcg tcctgtcggt cgcgccgctc atcggcgagg cgatcaagcg catccatcgc
     3061 ggcgagtcgg tgggcgcgct gttctcgtcc gaggtgtcgt tcactcagga gatgctcctg
     3121 tgggaggacg gcccgatcca gcccggccgc gagtccccgg ccggcgacgg cgacgtacca
     3181 tcacccatgg ccgcttcggc cggcgcctga ccgcagcacc ggagtcccat gagcctgcag
     3241 ctgcaccgcc cggacgggaa gggcggactc gagcctcgac cggtcagcga cgagaactgg
     3301 cacagccagc tccgctcgcc gcgctggggc gcgtcgctcc ggggcggccg cctgccaagg
     3361 ctcgccaacc ccgagatgaa ccccacctcg accgcgaagt cggtggcgtt ctggctggtc
     3421 ctggggctgc tcacgttcgg cgtgatcgtg gtcgggtacg gcagcggatt cttcaagttc
     3481 gtcacggcat aggcgttcgg cgcgtcgtcg gatgagcgcg tcgcgaccgt tcgggaaccc
     3541 gatcgccgtc gacgacgtcc gggagccacg atgcagcgag tcctgatcgc ctccggcgtc
     3601 ctcggcctgg gcacggccct cgtgttcggc gccgccgcgg tcaccgcgac cctgtttccc
     3661 aagggcccga tggtggccgc cggcatgaac gagatctgga tgcgcaacgg cggcggcgtg
     3721 atgatgggcg tgggcaagcc gatgccgatg ccggcgcccg gttttgccat cgatgacggc
     3781 aaagacgtca tcgacctgcc cggcgtccag cagtagccca tcgcctgaca ccggctcggt
     3841 cgcgtcggcc gggggatcag cggacccacc ttatactctc ccggccatgc gcttgctctc
     3901 ccgattcttc gataccaacg accgcgagct gacccgcctc cagccactcg tcgaccgcac
     3961 caacgagctc gagcccgagt tcgccgccct ctcggacgac gagatccgcg agcgaatgga
     4021 cgcgatccgc gcggagatcg cggaggacgc ggcccccgag gaac
//
LOCUS       DBNHXO010000199         4453 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1840424, whole genome shotgun sequence.
ACCESSION   DBNHXO010000199 DBNHXO010000000
VERSION     DBNHXO010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1840424"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="AC162A_04275"
     CDS             <1..280
                     /locus_tag="AC162A_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010088.1"
                     /translation="LPGQRQMPPARTGFGLPNLRRNVCPHFYMTGPRGAIPIQAPHLL
                     KGLLRQPNNVSRCQLRGGVHLDEGYVNDVCLTPRFNQCVFYEDDLTKS"
     gene            277..1674
                     /locus_tag="AC162A_04280"
     CDS             277..1674
                     /locus_tag="AC162A_04280"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR5010089.1"
                     /translation="MSARGERQDPRVAPDLSYAEALAAIESRGRYGVRLGLGRVRALL
                     RAMGDPQLAVRGALVAGTNGKGSVIALAGSALRAAGYRVGETPKPHLVTYRERIVVDG
                     RPIDAGSFARLVGTVIPLADRISPRLGPPTEFELLTAVMFRHFADAGLDVALVEVGLG
                     GRLDATHAWDGGVAVITNVGLDHTDRLGTTVTAIAREKAAIIERGDVAVTGATGDALT
                     VVRRRSARMGVPLTIAEPAPILDWDRDAQWVELPGLGATAVALRGRHQAANVAVADSV
                     LDALETAGIARVPADARRRGYASATWPGRLELVQAARPGGGTREVLLDGAHNPDGALA
                     LAQALADLRPYLAGGGASPPRPLVLVWASMADKDVRGVLAAVAASPAVADATIVCTTL
                     DLPRALPAAALADAWRAALPGGRVLIERDPAAALDLALATGDGPVVVAGSLYLVGAAR
                     ARLVDDPALRDPVAA"
     gene            1671..2555
                     /gene="folP"
                     /locus_tag="AC162A_04285"
     CDS             1671..2555
                     /gene="folP"
                     /locus_tag="AC162A_04285"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR5010090.1"
                     /translation="MIAGQPGASGATRIGPRTFDWGRRTHLMGVLNATPDSFSGDGLL
                     AATDPVAAAVEQARAMVADGADLLDVGGASSRPGHEPVAPADEIARVVPVIRAIVADL
                     PAVPVSIDTTSPQVAAAALDAGAHLLNDVWGVSDDPAMARLAAARGVPIVLMHNRALP
                     DYRDLVAEVIADLRAALERALDAGIAPDALIVDPGFGFGKTPEHNITLLANLGALRSL
                     GRPVLLGTSRKSTLGRLLDVPADQRVEATVATTVVGVMAGVDIVRVHDVRADARAARV
                     ADAVARGWRPDGWPGATP"
     gene            2552..2917
                     /gene="folB"
                     /locus_tag="AC162A_04290"
     CDS             2552..2917
                     /gene="folB"
                     /locus_tag="AC162A_04290"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR5010091.1"
                     /translation="MTDRIVLDGMVFQGTHGVYPEEHERPQRFEVDVELALDLAPAGR
                     SDDLAQTIDYGLVFETCRRIVESTRFDLIEALAEAIANEILAGFAADEVTVRVRKPEV
                     RLGGTLRFAGVEIRRRRTG"
     gene            complement(2941..4416)
                     /locus_tag="AC162A_04295"
     CDS             complement(2941..4416)
                     /locus_tag="AC162A_04295"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR5010092.1"
                     /translation="MNDEPVSDAPQGGGQPDPLDDTQRVEVPRYAPTPDPRPDARWAW
                     ATSGEQAAPSPWQDPATRPVGTPSAPVADYGTGAASQPGPAATWDTMPGNAGSTSPGW
                     GPPAQVETAPVVPTRRRGGAGIGTIVTVSVLSAVLASGGTVLLLDRTGAFEKSAPAST
                     IGSGQQTGSKLPVTIDESSAVIDAAAKVGPAVVKIDTEGSGSDPFQQATQGVGSGVIY
                     DPNGWILTNRHVIADAAKLTVDLRDGRSFPGTVYGIDTLTDLAIVKIDQTGLPTAPIG
                     HSDGLKVGQLVIAIGSPLGMYSFSVTSGIVSGKGRTVTVDDGKRISNLIQTDAAINPG
                     NSGGPLVDAAGSVVGINTAVATDSSGIGFAIPVDIARPIMDQALKGEPLSRPWIGVRF
                     SPIDKQLQKERNLSVENGALVTTIGGSEPAITPDSPADKAGLKDGDVIVSINGTTIDQ
                     EHPLDALLVQYAPNDTITLSVLRDGSTVLVKVKLGTRPKDL"
BASE COUNT          537 a         1594 c         1670 g          652 t
ORIGIN      
        1 cctccccggg caacgccaga tgccgccggc gcggacgggc ttcggcctcc ccaacctgcg
       61 gcgcaacgtc tgcccgcact tctacatgac cggcccccgt ggcgcgatcc cgatccaggc
      121 gccgcacttg ctcaagggcc tgctgcggca gcccaacaac gtctcgcgct gccagctccg
      181 tggcggggtc cacctcgacg agggctacgt gaacgacgtg tgcctgaccc ctcggttcaa
      241 ccagtgcgtg ttctacgagg acgacctgac gaagtcgtga gtgcccgcgg cgagcgacag
      301 gacccgaggg tcgcccccga cctcagctac gccgaggcgc tggcagcgat cgagtcgcgc
      361 ggccgctacg gggtgcgtct cgggctcggc cgggtgcggg cgctgctccg ggcgatgggc
      421 gatccgcagc tcgccgtccg cggcgcgctc gtcgccggca ccaacggcaa gggcagcgtg
      481 atcgcgctcg cgggctccgc cctgcgggcc gccggctacc gggtgggcga gacgcccaag
      541 ccgcatctcg tcacctaccg ggagcggatc gtggtcgacg ggcggccgat cgacgccggt
      601 tcgttcgcgc ggctggtggg aacggtcatc ccgctggccg accgcatctc gccgaggctc
      661 ggaccgccga ccgagttcga gctgctgaca gcggtgatgt tccggcactt cgcggacgcc
      721 ggcctcgatg tcgcgctggt cgaggtcggg ctcggaggca ggcttgacgc cacgcacgcg
      781 tgggacggcg gcgtcgcggt gatcaccaac gtcgggctcg accacaccga tcgcctgggg
      841 acgaccgtga cggcgatcgc gcgcgagaag gcggcgatca tcgagcgcgg cgacgtggcc
      901 gtcaccgggg cgaccggtga cgcgttgacg gtcgtccggc ggcgatccgc ccggatgggc
      961 gtgccgctga cgatcgcgga gccggcgccg atcctcgact gggatcgtga cgcccagtgg
     1021 gtcgagctcc cggggctggg cgcgacggcc gtggcgctcc gcggccgtca ccaggcagcc
     1081 aacgtggccg tcgccgattc tgtcctggac gcgctggaga ccgcgggcat cgcgcgcgtt
     1141 ccggcagacg cccggcgtcg tggctatgcg agcgcgacgt ggccgggccg gctggagttg
     1201 gtgcaggccg cccgcccagg cggtggcacg cgagaggtcc tgctcgacgg ggcgcacaac
     1261 ccggacggtg cgctcgccct cgcgcaggcc ctggccgatc tgcgaccgta cctcgcaggc
     1321 ggcggcgcga gcccgccgcg cccactggtc ctggtctggg cttcgatggc cgacaaggat
     1381 gtgcgaggcg tcctcgcggc ggtcgccgcc agccccgccg tcgccgacgc cacgatcgtg
     1441 tgcacgacgc tcgacctccc gcgggccctc ccggcggcgg ccctcgcaga cgcgtggcgc
     1501 gccgcgctgc cgggtggccg ggtgctcatc gagcgggacc cggcggccgc gctcgatctt
     1561 gccctcgcga ccggcgacgg gccggtcgtg gtcgcggggt cgctgtatct cgtcggggcc
     1621 gcgcgagcgc gcctcgtcga cgatcccgcc ctgcgcgatc cggtcgcggc atgatcgccg
     1681 gtcagccggg tgcgtccggc gccacccgta tcggtccccg gaccttcgac tggggacgtc
     1741 ggacccacct gatgggcgtg ctcaacgcga cccccgattc gttctcgggc gacgggctgc
     1801 tggccgccac ggacccggtc gcggccgccg tcgagcaggc ccgcgcgatg gtcgccgatg
     1861 gcgccgacct gctcgatgtc ggtggcgcgt cgagccgtcc gggccacgaa cccgtcgcgc
     1921 cggcggacga gatcgcgcgg gtcgtcccgg tcatccgcgc gatcgtcgcg gacctgccgg
     1981 ccgtgcccgt ctcgatcgat acgacgagcc cgcaggtcgc tgcggccgcc ctcgacgccg
     2041 gcgcgcacct gctcaacgac gtgtggggcg tgtccgacga cccggcgatg gctcgcctgg
     2101 cggccgcgcg tggcgtgccg atcgtcctga tgcacaaccg cgcgctgccc gactaccgcg
     2161 accttgtcgc cgaggtcatc gccgacctcc gggccgcgct cgagcgggcg ctcgacgccg
     2221 gcatcgcgcc ggacgcgctg atcgtcgacc cggggttcgg gttcggcaag acacctgagc
     2281 acaacatcac cctgctcgcc aatctcgggg ccctccgctc gctgggccgt ccggtcctgc
     2341 tcggcacctc gcgcaagtcg acgctgggca ggctgctgga cgtgccggcc gatcaacgcg
     2401 tcgaggcgac cgtggcgacc acggtcgtgg gcgtcatggc gggcgtggac atcgtccgcg
     2461 tgcacgacgt gcgcgcggac gcacgggcgg cgcgcgtcgc cgacgcggtc gcgcgtggct
     2521 ggcggccgga cggctggccc ggggccaccc cgtgaccgac cggatcgtgc tcgatgggat
     2581 ggtgttccag gggacacatg gcgtctaccc cgaggagcac gagcggcccc agcgcttcga
     2641 ggtcgacgtg gagctggcac tcgacctggc gccggcgggc cgcagcgacg acctcgccca
     2701 gaccatcgac tacggcctgg tgttcgagac gtgccgccgg atcgtggagt cgacgcggtt
     2761 cgacctgatc gaggcgctcg ccgaggcgat cgcgaacgag atccttgccg ggttcgcggc
     2821 ggacgaggtc accgtccgcg tgcgcaagcc ggaggtgcgg ctcggcggca cgctgcgctt
     2881 cgcgggcgtc gagatccggc ggcgccggac cggctgaccg gccggcgcgc ggccggcgcg
     2941 ctacaggtcc ttcggccgcg tcccgagctt gaccttgacc agcacggtgc tcccatcgcg
     3001 gagcacgctc agcgtgatcg tgtcgttggg tgcgtactgc accaggagcg cgtcgagcgg
     3061 gtgctcctgg tcgatggtcg tgccgttgat cgagacgatc acgtcgccgt ccttgaggcc
     3121 cgccttgtcc gcggggctgt cgggggtgat ggccggctcg ctgccgccga tcgtcgtgac
     3181 cagcgcgccg ttctcgacgc tgaggttgcg ttccttctgg agctgcttgt cgatcggcga
     3241 gaaccggacg ccgatccacg gacgagaaag cggctcgccc ttgagggcct ggtccatgat
     3301 cggacgcgcg atgtcgaccg ggatggcgaa cccgatcccg ctcgagtcgg tcgcgacggc
     3361 cgtgttgatg ccgacgaccg atcccgcggc gtcgacgagc ggcccgcccg agttgcccgg
     3421 gttgatcgcc gcgtcggtct ggatcaggtt ggagattcgc ttgccgtcat cgactgtgac
     3481 cgtgcggccc ttgcccgaca cgatgccact cgtcaccgag aacgagtaca tgccgagcgg
     3541 gctgccgatc gcgatcacca gctgccccac cttgagaccg tcggagtggc cgatcggggc
     3601 cgtcgggagt ccggtctggt cgatcttgac gatggcgagg tcggtgaggg tgtcgatccc
     3661 gtacacggtg cccgggaagg agcgcccgtc ccgcaggtcg acggtcagct tggcggcgtc
     3721 ggcgatgacg tgtcggttgg tgaggatcca gccgttcggg tcgtagatga cgcccgaccc
     3781 gacgccctgc gtggcctgct ggaacggatc gctgcccgag ccctccgtgt cgatcttgac
     3841 gaccgcgggg ccgaccttgg cggcggcgtc gatgacggcg gacgactcgt cgatcgtgac
     3901 gggcagcttg ctgccggtct gctggccgct gccgatcgtc gacgcggggg cggacttctc
     3961 gaacgcgccc gtccgatcca gcagcaggac cgtgccgccc gacgcgagca cggccgacag
     4021 caccgagacc gtgacgatcg tgccgatgcc cgctccgccg cggcggcggg tggggacgac
     4081 gggcgccgtc tcgacctgcg cgggcggccc ccacccgggc gacgtagagc cggcgttgcc
     4141 gggcatcgtg tcccaggttg cggcggggcc gggttgcgag gcagccccgg tgccgtagtc
     4201 agcaacgggt gcggacggcg tgcccaccgg cctcgtcgcc gggtcctgcc aggggctcgg
     4261 agccgcctgc tcgcctgacg tcgcccatgc ccaccgcgcg tcgggacgcg ggtcgggcgt
     4321 cggggcgtac ctcgggacct ccacgcgctg cgtgtcgtcg aggggatcgg gttgaccgcc
     4381 gccctgggga gcgtcggaca cgggctcgtc gttcatggct gcggggactg cttctctggc
     4441 tggacgccgg tgc
//
LOCUS       DBNHXO010000200         2170 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4816666, whole genome shotgun sequence.
ACCESSION   DBNHXO010000200 DBNHXO010000000
VERSION     DBNHXO010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4816666"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..517)
                     /locus_tag="AC162A_04300"
     CDS             complement(71..517)
                     /locus_tag="AC162A_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010093.1"
                     /translation="MSREPGLDRRGRQPVWSGLFTDGLAARIPPPVRPLALTSIKAIH
                     TVIFASIAALVAAVAWDGVRGLPRRRTAFAAAVAIAESIVYASNNRVCPLTPLAEELG
                     AANGSVTDMFLPDAVSRLIPAVAGSALGVGLVLNVRALRREDSTSR"
     gene            complement(514..1389)
                     /locus_tag="AC162A_04305"
     CDS             complement(514..1389)
                     /locus_tag="AC162A_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010094.1"
                     /translation="MSAQTLHVRLSITSSETTGPADLLAGIALVLAAVSAVAGLVVPG
                     LYRDSDAWIRQARAADLVTLLAVAPILAVAIWRARAGSAVGRLAVLAALGYLAYTYAI
                     FGFSVAINPMTPVHLAVLGLSVWSLLYGVIELARHPLATDTARGLPRRPAVALLLAVP
                     VLFAAMWLAQIAQSIATGAPPAALAQAGLSTNPVYTLDLAFALPFLALAGVAVARRSQ
                     FGGQLGLAASMWAALMGLGVLAIFAFDAAAGAPVEVGVFVLIAGITFAATALALVAIV
                     DPRRRSVASQAHPRA"
     gene            complement(1411..1725)
                     /locus_tag="AC162A_04310"
     CDS             complement(1411..1725)
                     /locus_tag="AC162A_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010095.1"
                     /translation="MFTTSAAVWLNGRHATVARTDPDGGITTVEVDRGVEPETSYLVR
                     VVRVIGDRERVLILGPSSPRLALEREYVTVFKRPDRLIDVEAAGPTDPTALVKRLTTL
                     LS"
     gene            complement(1835..>2170)
                     /locus_tag="AC162A_04315"
     CDS             complement(1835..>2170)
                     /locus_tag="AC162A_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5010096.1"
                     /translation="ATLQVGGRPEVFALGDATDLPLSKAGSTAHFEAPVVVERIVAAV
                     EGRGVDHRHGDYLGKVMCFFEIGDGKGTLLQFDYDHPPRPPKPNRFWHLGKIVFNKTY
                     WHTVPKGRV"
BASE COUNT          348 a          740 c          819 g          263 t
ORIGIN      
        1 ggaggaggcc gcaccctcga gggagcttcg gttcctgcga cggtgcgacc gcggatggtg
       61 tgacgcggcg tcagcgcgaa gttgaatcct cgcgtcgcag tgcgcggacg ttgaggacga
      121 ggccgactcc gagggcgctt ccggcgacgg cggggatgag gcggctgacg gcgtccggca
      181 ggaacatgtc ggtcaccgag ccgttcgcgg cgcccagctc ctcggccagc ggggtgagcg
      241 gacagacacg gttgttgctg gcgtagacga tggactccgc gatggcgacc gcggcggcga
      301 aggcggtccg ccggcgcggc agcccgcgga cgccgtccca ggccacggcg gcgaccagcg
      361 cggcgatcga ggcgaagatc accgtgtgga tcgccttgat cgaggtcagg gcgagcggcc
      421 gcaccggggg cgggatgcgc gccgccaggc catccgtgaa cagcccgctc cagaccggct
      481 gccgcccgcg tcgatccagc ccaggttccc ggctcatgcg cgcggatggg cctgcgacgc
      541 caccgagcgg cgccgagggt cgacgatcgc aacgagggcg agggccgtcg cggcgaacgt
      601 gatcccggcg atgagcacga acacgccgac ctcgaccggc gccccggccg ccgcatcgaa
      661 cgcgaagatc gccagcacgc ccaggcccat gagcgccgcc cacatcgagg cggccagacc
      721 aagctgccca ccgaactgcg accgcctcgc caccgcgacg ccggcgagcg cgaggaatgg
      781 aagagcgaac gcgaggtcga gggtgtacac ggggttggtc gacagcccgg cctgggccag
      841 cgccgcgggc ggcgcgcccg tggcgatcga ctgggcgatc tgggcgagcc acatcgcggc
      901 gaacagcacc ggcacggcga gcagcagggc aaccgccggg cgtctcggca gccccctggc
      961 ggtgtcggtt gccagcggat gccgggcgag ctcgatcacg ccatacagca gcgaccagac
     1021 cgacaggccg aggacggcga ggtggaccgg cgtcatcggg ttgatcgcga ccgagaaccc
     1081 gaagatcgcg taggtgtaag cgaggtaccc gagggccgcg agcaccgcga gccgtccgac
     1141 cgcggatccg gcccgcgccc gccagatcgc gaccgccagg atcggcgcga cggcaagcag
     1201 cgtgacgaga tcggcggcgc gcgcctggcg aatccaggcg tcgctgtcgc ggtagaggcc
     1261 cgggacgacc agtccggcga cggccgagac ggccgcgagc acgagggcaa tccccgcgag
     1321 gaggtcggcc gggcccgtcg tctcagacga ggtgatggag aggcgcacgt gcagtgtctg
     1381 ggcgctcacg ttcgtggtcc cgttatgggg tcaggagagc agcgtggtca ggcgcttcac
     1441 gagtgccgtc gggtcggtcg ggccggctgc ctcgacatcg atcaggcgat ccggccgctt
     1501 gaagaccgtg acgtactcgc gctcgagggc gagtcgtggc gagctcggac cgaggatcag
     1561 gacccgctcg cggtcgccga tcacccggac gactcgtacc aggtacgagg tctcgggctc
     1621 gacgccgcga tcgacctcga ccgtcgtgat cccgccatcg gggtccgtcc gggcgacggt
     1681 cgcatgacgg ccgttgagcc agaccgctgc gctggtggtg aacatctcgg atacctcctg
     1741 gtcaccacgc tagccccggc caggccgccg gccccatggc cgatcggccc gcgcttgggg
     1801 gccgagtgac acgaatgggg cgggcacctg acggtcagac gcgacccttg ggaaccgtgt
     1861 gccagtaggt cttgttgaac acgatcttgc cgaggtgcca gaaccggttc ggcttgggcg
     1921 ggcgcggcgg gtggtcgtag tcgaactgga ggagcgtccc cttgccgtcg ccgatctcga
     1981 agaagcacat gaccttgccc aggtaatccc cgtgccggtg atcaaccccc cggccctcga
     2041 cggcggcgac gatccgctcg accaccacgg gggcctcgaa gtgggccgtc gacccggcct
     2101 tggacagcgg caggtccgtc gcgtcgccca gggcgaagac ctcgggccgc ccgccgacct
     2161 ggagcgtggc
//
LOCUS       DBNHXO010000201         2099 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3626284, whole genome shotgun sequence.
ACCESSION   DBNHXO010000201 DBNHXO010000000
VERSION     DBNHXO010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3626284"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="AC162A_04320"
     CDS             complement(<1..260)
                     /locus_tag="AC162A_04320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR5010097.1"
                     /translation="MRRNLKPGDLGDLLDRPLNAILGLHRADGSILLTPVWHLFKDGS
                     FYFQMPGGDRKIAMLERDPRCSMLVAENEHPYRGIEVQGVGRT"
     gene            complement(296..1966)
                     /locus_tag="AC162A_04325"
     CDS             complement(296..1966)
                     /locus_tag="AC162A_04325"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR5010098.1"
                     /translation="MTGFVGRVRATLRVAAEVLHNPSLRRVGLAFLAFGAVEYGSWVA
                     ILLYAYEATGPASVGVVALVQLVPAALFAPPAATLSDRFRREKVLFVAYGLLALLTGL
                     TGFGMLAGWPPLLVYAAAVSVVMPLSMIRPTHNALLPTLARSPSELTAANAATSIAEA
                     AGLLLGPLTAAAILLFAVPGTVLAVMACVAGLGALLVAGLRPAGLLVASADDERDAST
                     GLAVLGGFRALAADGDARLVVAILSARQLMIGVTDVLFVLLAIELYQTGDSGAAILSA
                     AIGAGGIIGGAAAFGLIGRPHIAPVLLASGLLWGLTFAVIAFAPGAVAPLVLVVGGVG
                     LAVMDVAGRTVLQRAVADEVLARVFGILEGLMMAALAVGSILVPVVVALAGLQWSVVV
                     FAAMLPLLLLLTSPGLRSIDRRADVPVRAIGLLLKLRMFEVLDPPSLESLGRAASWLT
                     VPGGTTIIREGDPGDQFYVLASGAVDVTRHDHPVRRLDAVGDGFGEIALLRDVPRTAT
                     ITAAVETELLILGRREFLGAVTGHPVARDMADRAVDEVLRADQEAGEA"
BASE COUNT          359 a          791 c          724 g          225 t
ORIGIN      
        1 gtccggccca cgccctggac ctcgatccct cgatacgggt gctcgttttc ggcgacgagc
       61 atgctgcagc gcggatcgcg ctcgagcatc gcgatcttcc ggtcgccacc gggcatctgg
      121 aagtagaacg atccgtcctt gaacaggtgc cagacgggcg tcaggaggat cgatccgtcc
      181 gcacgatgca agcccaggat ggcgttcaac ggacgatcca ggagatcgcc gaggtcgccc
      241 ggcttgaggt tccgtcgcac cgcggaagtg tagtgcgcga cgcggcgacc agcgcctatg
      301 cctcgcccgc ctcctggtcg gcgcggagca cctcgtcgac ggcccggtcg gccatgtcgc
      361 gcgcgaccgg atggccggtg accgcgccca ggaactcccg gcgcccgagg atcagtagct
      421 ccgtctcgac cgccgccgtg atggtcgcgg tccgcggcac gtcgcgcagc agtgcgatct
      481 caccgaaccc gtcgccgacc gcatccagcc gccgcaccgg atggtcgtga cgggtgacgt
      541 cgaccgcgcc cgaggccagc acgtagaact ggtcgcccgg atcgccctcg cggatgatcg
      601 tggtcccgcc ggggaccgtc agccacgagg cggcgcggcc aagggactcc agggacggtg
      661 ggtccagcac ctcgaacatg cggagcttca gcaacaagcc gatggcgcgc accgggacgt
      721 cggcgcgtcg gtcgatcgac cgcaggccgg gcgatgtcag caggagcagg agcgggagca
      781 tcgccgcgaa cacgacgacc gaccactgga ggccggccag cgccacgacc accggcacca
      841 ggatcgaccc gacggcgagc gcggccatca tcaggccctc gaggatgccg aacacccgcg
      901 ccagcacctc gtccgcgacc gcgcgctgga ggaccgtccg cccggcgacg tccatcaccg
      961 ccagcccgac gccgccgacc accaacacga gcggcgccac agcgcccggc gcgaacgcga
     1021 tcaccgcgaa cgtgaggccc cacagcagcc cactcgcaag cagcaccggc gcgatgtgcg
     1081 gccgcccgat cagcccgaac gccgccgcgc cgccgatgat ccctccggcc ccgatcgccg
     1141 ccgacaggat cgcggcccct gagtcgccgg tttggtacag ctcgatcgcg agaagcacga
     1201 acagcacatc ggtcacgccg atcatcagtt gccgcgccga caggatcgcc acgaccaggc
     1261 gggcgtcgcc gtcagcggcc agcgcccgga acccgccgag gaccgccaag ccggtggacg
     1321 catcgcgttc atcgtccgcc gaagcaacca acagcccggc cggccgaaga ccggcgacga
     1381 gcagcgcccc caggccggcc acgcaggcca tcaccgcgag cacggttccg ggcaccgcga
     1441 agagcaggat cgccgccgcg gtcagcggac ccaggagcag cccggccgcc tcggcgatgc
     1501 tcgtggcggc gttggccgcg gtcagctccg aggggctgcg ggcgagggtg gggagcaggg
     1561 cgttgtgggt cggtcggatc atcgacagcg gcatcaccac cgagacggct gcggcataga
     1621 cgagcagtgg cggccagccg gccagcatcc cgaacccggt gaggccggtc aggagcgcca
     1681 gcagcccgta cgccacgaac agcaccttct cgcgccggaa ccggtcgctg agcgtcgcgg
     1741 ccggcggtgc gaacagggcc gcgggcacca gctggacgag cgcgaccacg cccaccgacg
     1801 ccgggccggt ggcctcgtac gcgtacagga ggatcgcgac ccagctcccg tactcgaccg
     1861 cgccgaacgc gaggaacgcc agcccgaccc ggcgcagcga cgggttgtgc agcacctccg
     1921 cggcgacacg cagggtcgcc cgcacccgac cgacgaagcc tgtcatccgg ccccagtgtg
     1981 ccagccgagg tgcgcccacc gggccgccgt cttggcgggt gagcgcaagg caggggacaa
     2041 tggccccatg gcgatcctta ccgccgacga gctcgcgtcc gtccgccgcg agtatcggt
//
LOCUS       DBNHXO010000202         5435 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4619253, whole genome shotgun sequence.
ACCESSION   DBNHXO010000202 DBNHXO010000000
VERSION     DBNHXO010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4619253"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="AC162A_04330"
     CDS             complement(<1..535)
                     /locus_tag="AC162A_04330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015184933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5010099.1"
                     /translation="MTGKTIGLPDNVGQYVVRWGVNEAPILGRLREETASHPRSQMQI
                     APEQGALLAILVELLDARQYVEIGTFTGYSSLAVLLAMPPDGRIVCCDISEEYTSVAR
                     RYWAEAGVADRADLRIGPAVETLDALLAEGRAGTFDLAFIDADKAAYPAYYERCLELV
                     RPGGLVALDNVLWSGDVA"
     gene            complement(554..1246)
                     /locus_tag="AC162A_04335"
     CDS             complement(554..1246)
                     /locus_tag="AC162A_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5010100.1"
                     /translation="MGRSILVVDDETTLRETLVEALELEGYRAIPAADGRQALALFRS
                     ERPDLVLLDLMLPELSGVEVCRIMRAESGVPIIMLTAKDAEVDKVVGLELGADDYVTK
                     PFSLRELTARIRAIFRRAEQVAAADVPPVVDLGRVQVDLAGHRVLRDGEAVSLKPKAF
                     DLLAFLLRHPGQVFTRDQLLEHVWGYDYGGESRTVDVHIHWLRSEIEADPAAPEYLHT
                     VRGVGYVFRRPA"
     gene            complement(1327..2829)
                     /gene="gltX"
                     /locus_tag="AC162A_04340"
     CDS             complement(1327..2829)
                     /gene="gltX"
                     /locus_tag="AC162A_04340"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR5010101.1"
                     /translation="MASDIRVRIAPSPTGPLHLGTARTALFNFLFARHTGGTFVLRLE
                     DTDVARSTEAFEKDILDGLHWLGITWDEGPGVAGIEEAGPYAPYRQMQRLDRYAEASA
                     RLLAADLAYPCFCTPEELDADRKAQEAAKLPPRYVGRCAVLTPDERAARVAKGTRAAT
                     RFRVRAGVVGWLDLVRDRIEIDTDNLGGDFVIVRADGTPLYHFTVVVDDAEMEISHVI
                     RGEDHVSNTPKHILLFEALGYDVPRFGHLPLILNPDGTKMSKRKSQTAVADYIAQGFV
                     REALVNYFAFLGWSPGTEEEVFTLEELGERFDLDKVQKGGARFDRERLEWLNGQWIRR
                     LDDDVLIERLTPFLEAAAIAGEIDRTPSEGEVRAILPIVRERLPTLAAIVELMGYLWV
                     DDLEVDPALVVSKRWDAATTADGLAAARERLAAHDAVTWEAEELEPPLCELVEARGWK
                     AGDLFMAIRVATTGKTATPPLFDTLVALGRERTLARLDAAIARLSAPAAT"
     gene            2983..3756
                     /locus_tag="AC162A_04345"
     CDS             2983..3756
                     /locus_tag="AC162A_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010102.1"
                     /translation="MRGYTGIGRIDKRGHCEPGNLSPNGLDASRSIKLGDRIPELSRI
                     DLQPFVHDNLPVLAVAECRVHVDLAADRRVSAQAPDEVGGGAIKAREVRLKAEATHLL
                     GQPEICRRGVWREAHRVGREGETRTRLAHGVAGFAHEEEGVDEPEDGLTFHEHELSVG
                     DSPIRVGDHACALANDLRTKLDAAAEPRPLLRQRPLIREGRDDRIQALVDRFGSVDCS
                     IDIVGNDEPERVAERVALGIHLRLQVTEVDARCRAEGLG"
     gene            complement(3931..4662)
                     /locus_tag="AC162A_04350"
     CDS             complement(3931..4662)
                     /locus_tag="AC162A_04350"
                     /inference="COORDINATES: protein motif:HMM:NF015956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR5010103.1"
                     /translation="MQRRHDPADISAAAQEYLLALRVMGSDGRVATTAQLGRHVGVTT
                     QAASEMCRRLAADGLVEPATGRGLSLTAAGRAAADAIFRRHALLEWLLTAVVGLTWAE
                     SDVEAMRLQGALSPRVESKLDELLGHPETCPHGNPIDLETARRRPAGVPLSELESGSK
                     ATVLRITEEAEEDAGLLSYLEARALTPGAPVTVLARSESLDSLTLDGPRGRATLGLRP
                     AALIRVLPGVADPALFHKVPAPLGG"
     gene            complement(4704..>5435)
                     /locus_tag="AC162A_04355"
     CDS             complement(4704..>5435)
                     /locus_tag="AC162A_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010104.1"
                     /translation="QARARRAAQQEAGTADPDGGIPGAGGPNADPGAPSWGFGGGSTT
                     TDGDGGWGTHGWAYEEAADAADPGARNPAGRPDTAGAWATPTEPLPDLEAVARRAAPR
                     NLLALARRPDRRWRLLVALLAWPPVGYGVGTLLATSTGCAQFSAACPEPLPLLSLAVQ
                     PLVIAALFAVPPAAAVGAFAALVALAVAIAVATVLSVSPGPSGGVGTGILGVAVTVAY
                     FVAMVGGAVAVWRPRPDDDAGARRT"
BASE COUNT          809 a         1939 c         1940 g          747 t
ORIGIN      
        1 cggcgacgtc cccgctccac aggacgttgt cgagcgcgac cagcccgccc ggccggacga
       61 gctcgaggca gcgctcgtag taggccggat aggcggcctt gtcggcgtcg atgaacgcga
      121 ggtcgaaggt cccggcccgc ccctcggcga gcagcgcgtc gagcgtctcc accgccggcc
      181 cgatccgcag gtcggcgcgg tccgcgaccc ccgcctccgc ccagtagcgc cgggccaccg
      241 aggtgtactc ctccgagatg tcgcagcaca cgatgcggcc gtcgggcggc atcgccagca
      301 gcaccgcgag cgacgagtag cccgtgaacg tgccgatctc cacgtactgc cgcgcgtcca
      361 gcagctccac gaggatcgcc agcagggcgc cctgttcggg cgcgatctgc atctgggatc
      421 gcgggtggct cgccgtctcc tcgcgcagcc ggccgaggat cggggcctcg ttgacgcccc
      481 agcggacgac gtactggccg acgttgtcgg gaaggccgat ggtcttgccg gtcacgcgat
      541 cgatggtgcc atgtcaggcc gggcggcgga agacgtaccc cacgccgcgc accgtgtgca
      601 ggtactcggg cgcggccggg tccgcctcga tctcgctgcg cagccagtgg atatgcacgt
      661 ccaccgtccg cgactcgccg ccgtagtcgt agccccacac gtgctccagc agctggtcac
      721 gggtgaacac ctggcccgga tggcggagca ggaacgcgag caggtcgaac gccttgggct
      781 taagcgagac cgcctcgccg tcgcgcagga cccggtggcc ggccaggtcg acctgtaccc
      841 ggccgaggtc gaccaccggc gggacgtcgg ccgccgcgac ctgctcggcg cgccggaaga
      901 tcgcccggat gcgggcggtc agctcgcgca ggctgaacgg cttggtcacg tagtcgtccg
      961 ccccgagctc gagcccgacc accttgtcga cctcggcgtc cttcgctgtg agcatgatga
     1021 tcggcacgcc cgattcggcg cgcatgatcc ggcagacctc gaccccggac agctcgggca
     1081 gcatcaggtc gagcaggacg aggtccgggc gctccgagcg gaacagggcg agcgcctggc
     1141 ggccgtcggc cgcggggatg gctcgatagc cctcgagctc cagcgcctcg accagcgtct
     1201 cgcgaagcgt ggtctcgtcg tcaacgacga gaatcgatcg tcccatgcac gccggatcgt
     1261 ggagtccggg cgttaacgcc gtgttcacca cgggccccgc ttcggccgtc ggtgttaacg
     1321 atccgttcat gtcgccgccg gggcactcag ccgtgcgatc gccgcatcaa gccgggcaag
     1381 cgtgcgctcg cggccgagcg ctaccagggt gtcgaacagc ggtggggtcg ccgtcttgcc
     1441 ggtcgtcgcg acgcggatcg ccatgaacag gtcgccagcc ttccagccgc gtgcctcgac
     1501 gagctcgcac agcgggggct ccagctcctc ggcctcccag gtcaccgcgt cgtgggcggc
     1561 caggcgctcg cgcgcggccg cgaggccgtc cgcggtcgtc gccgcgtccc agcgcttgga
     1621 cacgacgagg gccgggtcca cctcgaggtc gtcgacccac aggtagccca tcagctcgac
     1681 gatcgcggcc agggtcggca ggcgctcgcg gacgattggc agaatggcgc ggacctcacc
     1741 ctcggacggg gtccggtcga tctccccggc gatcgcggcc gcctcgagga acggcgtcag
     1801 gcgttcgatc aggacgtcgt catccaggcg ccggatccat tgcccgttga gccactccag
     1861 ccgctcgcga tcgaaccgcg cgccgccctt ctggaccttg tcgaggtcga agcgctcccc
     1921 cagctcctcg agcgtgaaca cctcctcctc ggtgcccggt gaccagccca ggaacgcgaa
     1981 gtagttgacg agcgcctcgc gcacgaaccc ctgggcgatg tagtcggcga ccgccgtctg
     2041 gctcttgcgc ttgctcatct tggtgccgtc ggggttgagg atcagcggca ggtgaccgaa
     2101 ccgcggcacg tcgtagccca gcgcctcgaa cagcaggatg tgcttgggcg tgttgctgac
     2161 gtggtcctcg ccgcggatca cgtggctgat ctccatctcg gcgtcgtcga cgacgaccgt
     2221 gaagtggtac agcggcgtgc cgtcggcccg gacgatgacg aagtcgccgc cgaggttgtc
     2281 ggtgtcgatc tcgatccggt cgcggacgag gtcgagccag ccgaccacgc ccgctcgcac
     2341 ccggaacctc gtcgcggcgc gcgttccctt ggcaaccctg gccgcgcgct cgtcgggcgt
     2401 gagcaccgcg cagcggccga cgtagcgggg cgggagcttg gccgcttcct gcgccttgcg
     2461 gtcggcgtcg agctcctcgg gcgtgcagaa gcacggatac gcgagatcgg cggcgagcag
     2521 tcgggccgag gcctcggcgt agcggtcgag gcgctgcatc tggcggtagg gcgcgtacgg
     2581 cccggcctcc tcgatccctg ccacgcccgg gccctcgtcc cacgtgatcc cgagccagtg
     2641 caggccgtcg aggatgtcct tctcgaacgc ctcagtgctg cgggcgacgt ccgtgtcttc
     2701 caggcgcagg acgaacgtgc cgccggtgtg gcgcgcgaac aggaagttga acagggccgt
     2761 ccgcgccgtg ccgagatgca gcggccccgt ggggctgggg gcgatgcgga cgcggatgtc
     2821 ggaggccacg atccgattgt aggggcggtc gatgcgaggc ccgacgggga ggcaacctgt
     2881 tgagcgaagc gcgtccgctt acgggaccgc ggcaaggatc gcgttcacct cggggttgct
     2941 gaagcctccg aagacgaaca agcgatcccc tcgggacgcc aaatgcgtgg atacaccggg
     3001 attggacgca tcgacaaacg cggtcactgc gaacccggca acctgtcgcc gaacgggctt
     3061 gatgcctcgc ggagcatcaa gcttggcgac cgcatccccg aacttagccg gatcgacctg
     3121 cagcccttcg tacacgacaa tcttcctgtg ttggccgtcg ccgaatgtcg cgtccacgtc
     3181 gatctggcgg ccgaccgtcg ggtctccgcc caggcgccgg atgaggtcgg gggcggagcc
     3241 atcaaggcgc gtgaggtgcg gctcaaggct gaagcgaccc acctgctcgg gcagcccgag
     3301 atctgccgcc gaggggtgtg gcgggaagct caccgcgttg gccgtgaagg cgagacccgg
     3361 acacgcctgg ctcacggtgt cgccgggttc gcgcatgagg aagaaggtgt cgatgaaccg
     3421 gaggatggtc tcaccttcca cgagcatgag ctgtctgtcg gggacagtcc gatccgggtc
     3481 ggcgatcacg cgtgcgccct cgcgaacgac ctccgcacca aactcgacgc cgccgcggag
     3541 ccgcgaccgt tgctccgtca gcgtccgctc atcagggagg gccgcgacga tcgcatccaa
     3601 gcgctggtcg atcgcttcgg cagcgtcgat tgctccatcg acatcgtcgg caatgacgag
     3661 ccggaacgcg tcgccgagcg agttgccctc ggaatacacc tgcgcctcca ggtcacagag
     3721 gtcgacgctc ggtgtcgggc tgaggggctg ggttgacgag ggagtgggac tggggcgaag
     3781 gctcgccggc aagcatgcat tgagcgccag gacgacaacg actgtcgcgg ccgcacgccg
     3841 ccctaccgac atccggtgat cccctccagt ggtccgtcgc aatcagacca aaactgaccg
     3901 tagatgcaat gcgggagatc ccgcaagacg tcagccgccg agcggcgccg ggaccttgtg
     3961 gaagagcgcg ggatccgcga cgcccggcag tacgcggatc agcgccgcgg gccgcaggcc
     4021 cagcgtcgcg cgaccacgcg ggccgtcgag ggtaagcgag tcgagcgatt cgctgcgcgc
     4081 gaggaccgtc accggcgcac cgggggtgag cgccctcgcc tcgaggtacg agaggagccc
     4141 ggcgtcctcc tcggcctcct cggtgatccg gagcacggtc gccttcgagc ccgactccag
     4201 ctccgagagg gggacccctg ccggccggcg gcgcgccgtc tcgaggtcga tcgggttgcc
     4261 gtggggacag gtctcagggt ggcccaggag ctcatcgagc ttcgactcga ccctcggcga
     4321 cagcgcaccc tggaggcgca tcgcctcgac gtcgctctcc gcccaggtca gcccgacgac
     4381 cgcggtcagc agccactcca gcagcgcgtg gcggcggaag atcgcatcgg ctgcggcacg
     4441 cccggcggcg gtgagcgaca gcccgcgccc ggtcgcgggt tccacgaggc cgtccgccgc
     4501 gagccggcgg cacatctcgc tggcggcctg ggtcgtgacc ccaacgtgcc gaccgagctg
     4561 tgcggtcgtc gcgacccgcc cgtcagaacc catgacgcgc agcgcgagca ggtactcctg
     4621 cgctgcggcc gagatgtcgg cggggtcgtg acggcgttgc atgctcgcga gtgtagtgcc
     4681 gcccaccccc ggcgatgacg cgctcaggtc cggcgggcac ctgcgtcgtc gtctggccgc
     4741 gggcgccaga ccgcgaccgc gccgccgacc atggccacaa agtacgcgac cgtcaccgcc
     4801 acgccgagga tcccggtccc gacgcccccc gagggacccg ggctgaccga gagcacggtc
     4861 gcgaccgcga tggcgaccgc cagcgcgacg agcgcggcga acgccccgac ggcggccgca
     4921 gggggcaccg cgaacagggc cgcgatcacg agcggctgga cggcgagcga caggagcggc
     4981 aacggctcgg ggcaggcggc cgagaactgg gcacacccgg tcgacgtcgc aagcagcgtg
     5041 cccacgccat acccgaccgg cggccacgcg agcagggcaa cgagcagccg ccagcgacga
     5101 tcggggcggc gcgccagggc gagcagattc ctcggcgctg cacgacgcgc gacggcctcg
     5161 aggtccggga gcggctcagt gggagtggcc cacgcaccag ccgtgtcggg acgacccgct
     5221 gggttgcgtg cgccgggatc cgccgcgtcc gcggcctcct cgtacgccca tccgtgcgtg
     5281 ccccagcccc cgtcgccgtc ggtcgtcgtc gaaccgcccc cgaaccccca gctcggcgcg
     5341 ccggggtcgg cgttggggcc ccctgcgccg gggatccctc catcgggatc cgcggtgccc
     5401 gcttcctgct gggccgcgcg gcgggcgcgc gcctg
//
LOCUS       DBNHXO010000203         3944 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4223099, whole genome shotgun sequence.
ACCESSION   DBNHXO010000203 DBNHXO010000000
VERSION     DBNHXO010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4223099"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="AC162A_04360"
     CDS             complement(<1..1010)
                     /locus_tag="AC162A_04360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5010105.1"
                     /translation="MTLAVGELDGAALDDWDARAVDVPGGHVLQSRAWAEHRAAQGWQ
                     PRFLAIGEGRALALTRRWPLVGGGSAYLPRGPVGPGRPWTGDASGRETGAALVAAAAH
                     LQAAGIDVVAADPEVSADDAGYGEALAAAGFRAIAEIQPSRHRMALDLPEDGDAEAVM
                     AGVAKATRQRIRRAERDGVAVLRWDRASSGGSLDGAAPATEPPETAFGRFYELLRATG
                     DRRGFGFGGPAEFVTWWLRALASGHLVYLEAREGAADGDVLGGLVLYRHGRRLSTAHS
                     ADRAERRHDHPGAMHLLRWRAIELALAERRSEMDLGGVDVAGARREPLPGEPTYGLFE
                     HKR"
     gene            complement(1007..2122)
                     /locus_tag="AC162A_04365"
     CDS             complement(1007..2122)
                     /locus_tag="AC162A_04365"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HIR5010106.1"
                     /translation="MTAAWDAFVEANPLGSYLQLSGWAAVKAVNGWDVTRLHAPGAGT
                     GDGIGAQVLLRRPGPLPWAFAYAPRGPVLDRWDPEAIARFTELARAGLRSGGEGPGRV
                     SHLRIDPEIERGAGPDAGGAVAAGLTAAGWEATTPVQPISTRVIDLGADEAALWGDLR
                     KKWRQYVNKARTGGVRVVDAGPERLDDFYRIYRETADRAGFLIRAQSAYRDVWDAFGP
                     SGHARLLFAELADGTPVATLFLVRSGSRVVEPYGGMTEAGADSRANYLLKWEAIRSSK
                     EAGASTYDLWGLAHAGIAHFKTGFGGREVAYLGAFDLVLDPLGRRTYSIALRTRVRLA
                     RLRHGLRADGSTAAGYAGDGDGQRPNAPGTSDAGGDG"
     gene            complement(2119..3690)
                     /gene="murJ"
                     /locus_tag="AC162A_04370"
     CDS             complement(2119..3690)
                     /gene="murJ"
                     /locus_tag="AC162A_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR5010107.1"
                     /translation="MSASRNLARAGIIVSGAFLASRILGWFRVVVITNVYPTGPELDA
                     FFAAFRIPDFLFQLVAAGALSSALIPIIAGLLANEEEARAWRVVSSVTTLMLGTLAIL
                     AAVVLLLAPGLVATITPGFDAESLALTTELTRIMVLAPLFLAAGAVATSVLNARGRFG
                     AAAMAPLAYNLGIIGGALFLVPAFGVAGLAIGVVLGAAGHLLVQVPTMRRIGARIRPR
                     MDLGDPQARRTLVLMAPRALGLGATQVVFLVMTSLASTLPTGSITIFNFAFAILQIPI
                     GVIGVPLGIVLLPSLARQAATGGMDAFRHLLVRGLSTLAWVMIAITALGIALAHDVVQ
                     LLFGFVGVGQTALDETGTTLAIFLVGLTAHSLIAVLARAFYALQDTATPVLAALIAVG
                     VNILVATVLVGPLGLDGLAVAIATAAWLETLALVLLLKRRLPTLELGHVASVMVRTLI
                     VAVVGGAVAWAVTRGLETAFGTDPGFVRLLVQMALATAAGGLVILAGSLALRIEEPRL
                     IVGVVVDLVRRRGRS"
     gene            complement(3687..>3944)
                     /locus_tag="AC162A_04375"
     CDS             complement(3687..>3944)
                     /locus_tag="AC162A_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR5010108.1"
                     /translation="GVRGLRYRLDRIRRRAGLPDGVSPHTLRHSFATHLLDGGADLRV
                     VQELLGHESLATTQIYTHVSPTRLRSAYREAHPRARRDPAP"
BASE COUNT          577 a         1464 c         1471 g          432 t
ORIGIN      
        1 cgcttgtgct cgaacaggcc gtaggtcggc tcgccgggca gcggctcgcg tcgcgcgccg
       61 gcgacgtcca ccccgcccag gtccatctcg ctccgtcgct cggcgagcgc gagctcgatg
      121 gcccgccagc ggagcaggtg catcgcgccc gggtggtcgt ggcggcgctc cgcgcgatcc
      181 gcggagtgcg ccgtggacag gcgccggccg tgccggtaca gcacgagacc gcccagcacg
      241 tcgccgtccg ccgcgccctc gcgggcctcg aggtagacga ggtggccgga cgccagcgct
      301 cgcagccacc aggtcacgaa ctcggcggga ccgccgaacc cgaagcctcg ccggtcgccg
      361 gtggcccgga gcagctcgta gaagcgcccg aacgcggtct ccggcggctc cgttgccggc
      421 gcggcgccat cgagcgagcc tccgctcgaa gcgcgatccc agcggagcac cgccaccccg
      481 tcccgctccg cgcgccggat ccgctggcgc gtcgccttcg cgacgccggc catcacggcc
      541 tcggcgtcgc cgtcttcggg caggtccagc gccatccggt ggcgcgacgg ctggatctcg
      601 gcgatcgcgc gaaagccggc ggccgccagg gcctcgccgt agcccgcatc gtccgcgctc
      661 acctccgggt cggccgcgac gacgtcgatg cccgcggcct ggaggtgggc cgccgcggcc
      721 acgagggccg ctccggtctc gcgacccgag gcgtcgccgg tccagggacg ccccggtccg
      781 acggggccgc ggggcaggta cgccgacccg cccccgacca gtggccagcg gcgggtgagc
      841 gcaagcgccc ggccctcgcc gatcgcaagg aaccgcggtt gccacccctg ggctgcccga
      901 tgctccgccc aggcccgcga ctggaggaca tgcccacccg ggacgtcgac ggcccgcgcg
      961 tcccagtcgt cgagcgcggc accatcgagc tccccgaccg cgagcgtcac ccgtcgcccc
     1021 ccgcgtcgga cgtgcccggc gcgttcgggc gctgcccgtc gccgtcgccc gcgtagccgg
     1081 ccgcggtcga tccgtcggcc cgcagtccgt ggcgcaggcg ggccagccga acgcgcgtcc
     1141 gcagcgcgat cgagtaggtg cggcgcccga gcggatcgag cacgaggtcg aaggcaccga
     1201 gataggcgac ctcgcggccc ccgaagcccg tcttgaagtg ggcgatcccg gcgtgggcga
     1261 gcccccacag gtcgtaggtc gaggcgcccg cctccttcga gctgcggatc gcctcccact
     1321 tgaggaggta gttggcgcga ctgtcggcgc ccgcctcggt catgccgccg tagggctcga
     1381 ccacgcgcga gcccgatcgg accaggaaca gggtcgcgac gggggtcccg tcggcgagct
     1441 cggcgaacag gaggcgagcg tggccggacg gtccgaacgc gtcccagacg tcgcggtagg
     1501 ccgactgggc acgaatgagg aagcccgccc ggtccgccgt ctcgcggtag atgcggtaga
     1561 agtcgtcgag gcgctccggc ccggcgtcga cgacccgcac gccgccggtc cgcgccttgt
     1621 tgacgtactg gcgccacttc ttgcgcaggt cgccccacag ggcggcctca tcggcgccga
     1681 gatcgatgac ccgcgtcgag atgggctgaa ccggcgtcgt cgcttcccag ccggccgccg
     1741 tgagccctgc cgcgacggcg ccgccggcgt cgggaccggc gccccgctcg atctccggat
     1801 cgatccgcag gtggctgacg cggcccgggc cttcgccacc ggagcggagg ccggctctgg
     1861 cgagctcggt gaagcgggcg attgcctcgg gatcccagcg gtcgagcacg ggcccacgcg
     1921 gcgcgtaggc gaaggcccac ggcagcggcc cgggtcgccg gaggaggacc tgtgcgccga
     1981 tcccgtcgcc ggtgccggca ccgggggcgt ggagccgggt cacgtcccag ccgttgaccg
     2041 ccttcacggc cgcccagccg gagagctgga ggtaggagcc aagcgggttg gcctcgacga
     2101 aggcgtccca ggcggccgtc atgagcggcc gcggcggcgc acgaggtcga ccacgacccc
     2161 gacgataagc cgcggctcct cgatccgcaa cgcgagggat cccgccagga tcacgagccc
     2221 gccggcggcg gtcgccaggg ccatctgcac gagcagccgc acgaacccgg gatccgtccc
     2281 gaacgcggtc tcgagcccac gcgtgaccgc ccacgcgacg gcaccgccca cgacggcgac
     2341 gatcagcgtg cggaccatca cgctcgcgac gtgtccgagc tcgagggtcg gcagccggcg
     2401 cttcagcagg agcacgaggg ccagcgtctc gagccacgcg gccgtcgcga tcgcgaccgc
     2461 cagcccatcg aggcccagtg gcccgaccag caccgtcgcc acgaggatgt tcacgccgac
     2521 ggcgatgagg gcggcgagga ccggcgtcgc ggtgtcctgg agggcgtaga acgcgcgagc
     2581 aagcacggcg atcagggagt gggcggtcag cccgacgagg aagatcgcga gcgtggtgcc
     2641 ggtctcgtcc agcgcggtct ggccgacacc gacgaagccg aacagcagct ggacgacgtc
     2701 gtgggcgagg gcgatcccga gcgccgtgat cgcgatcatc acccaggcga gcgtggacag
     2761 cccgcggacg agcaggtgcc ggaacgcgtc catgcccccg gtcgccgcct ggcgcgcgag
     2821 cgatggcagc agcacgatcc ccagcggcac cccgatcacc ccgatcggga tctggaggat
     2881 ggcgaacgcg aagttgaaga tcgtgatcga cccggtcggg agcgtcgacg cgaggctcgt
     2941 catgaccagg aacacgacct gggtcgcgcc gaggccgagc gcccgcggcg ccatcaggac
     3001 cagcgtccgt cgcgcctgcg ggtcgccgag gtccatccgc gggcggatcc tggcgccgat
     3061 gcgccgcatg gtcgggacct ggacgagcag gtggccggcc gccccgagga ccacgccgat
     3121 cgccagcccg gcgacgccga acgccggaac gaggaacagc gccccaccga tgatgccgag
     3181 gttgtacgcg agcggcgcca tggccgccgc cccgaaccgc ccgcgggcgt tgaggacgct
     3241 cgtcgccacg gcgcccgccg cgaggaacag gggcgcgagg accatgatcc gggtcagctc
     3301 ggtcgtcagg gcgaggctct cggcgtcgaa gccgggggtg atcgtcgcca ccagcccggg
     3361 cgcgagcagc aggaccacgg ccgccaggat cgcgagggtc ccgagcatca gcgtcgtcac
     3421 gctcgagacg acgcgccacg cccgcgcctc ctcctcgttc gccagcagcc cggcgatgat
     3481 cgggatcagc gcggacgaga gcgcacccgc ggcgacgagc tgaaacagga agtccgggat
     3541 ccggaacgcg gcgaagaacg cgtcgagctc tggcccggtc gggtaaacgt tcgtgatgac
     3601 cacgacccgg aaccagccga ggatccgcga ggcgaggaac gcgccggaga cgatgatccc
     3661 ggcacgcgcg aggttgcgcg atgcgctcac ggggcggggt cgcgtcgcgc ccgagggtgc
     3721 gcctcccggt aggcggaccg gagccgcgtc ggtgacacat gcgtgtagat ctgcgtcgtg
     3781 gccaggctct cgtggccgag cagctcctgg acgacgcgaa ggtctgcccc gccgtcgagc
     3841 aggtgcgtcg cgaacgagtg gcgcagcgtg tgcggcgaca cgccgtcggg gaggcccgca
     3901 cgccgacgga tccggtcgag gcgatagcga agcccgcgga cgcc
//
LOCUS       DBNHXO010000204         2206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5215706, whole genome shotgun sequence.
ACCESSION   DBNHXO010000204 DBNHXO010000000
VERSION     DBNHXO010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5215706"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(635..1969)
                     /locus_tag="AC162A_04380"
     CDS             complement(635..1969)
                     /locus_tag="AC162A_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HIR5010109.1"
                     /translation="MEHDTGATALFDLEGVAVVEVVRGEDGTRTVHLATVDPAARACP
                     ACGTFATRVKERAVTRPRDLSHGGSGVQIRWHKRRWQCRETACERGSFTEQIAQIPAR
                     MRLTERLRTACGQAVVDGGRTVVQAGRDLGPSWPVVMRATSNFAERVLPAEPPACEAI
                     GIDETRRGRPVWKLDEDSGKWALEADAWHIGFVDAIGGHGLFGQVEGRNAGSVAAWLE
                     AQPTWWTDQVRYVAIDLCATFRSAIRTALPKATVVVDAFHIVQLAQRHLADLRRRLTW
                     KQHGRRARKGDAIYTVRKLLRRNAEDLTPDQHKLLASELTMMGTYGQQILAGWKAKEL
                     LRDVLRLTAKRTHTAPTRSTISAARYRFQAYCADHAFLPELATLAETVDQWWDGIEAY
                     VLTGITNAASEGNNRVIKLDARCAFGYRNRANQRLRSRSATTRRARREGVPG"
BASE COUNT          346 a          717 c          774 g          369 t
ORIGIN      
        1 ggagggagtc gatgtggctc agggtctcgg tgagcgcacg gcgcggttct ctcaccggca
       61 ggtctcctgg agtcaggggg ctgatgtcgc aaaccttaac cactgccagg atggggcagc
      121 agagcgagcg acagcgttat agccactcac ggatacaaca gttgacatac cttgctgacg
      181 gttgcaggat catgagcacg acgcagagga ccgatcgcca cagcacggct gcgcgccatc
      241 caacggcgtc aagctgcggg acagcctagt cgcacgacct gtcggacacg acccggtacc
      301 ccggcgagcg gaggtccgtg gcggtgacga tgaaaccgcc catgcgcatt acacgcggac
      361 acagattgcg gaggtacgac ttgttcgggt tggacgcgtc atccgaagct gtgtattaca
      421 cgatgctcaa gtccccccac atgcgcctcc cggagatcgc cgcagagctg aactggagcc
      481 ccgagcaggt caggcgcggg cttgacgagc tcgcccgcaa gtcgttggtg cactcctcat
      541 gggaagatca tggggccttg agggcggtgc cgccccaggt cggactggct gcgcttctcg
      601 aattgcagga gcgtgattta cggggtcttc gaatttaacc ggggaccccc tcgcgtcggg
      661 ctcgcctcgt ggtcgcggag cgtgaccgga ggcgttggtt ggcgcggttg cggtagccga
      721 aggcgcatcg ggcgtcgagc ttgatgacgc ggttgttgcc ctcgctggcg gcgttggtga
      781 tgccggtcag gacgtatgcc tcgatgccgt cccaccactg gtcgacggtc tcggcgagag
      841 tggccagttc ggggagaaag gcgtggtcgg cgcagtaggc ctggaaacgg tagcgggcgg
      901 cggagatcgt ggagcgggtg ggggcggtgt gggtgcgctt ggccgtgagg cggaggacgt
      961 cgcgcaggag ttccttggcc ttccagccgg ccaagatctg ttggccgtag gtgcccatca
     1021 tggtcaactc gctggcgagg agcttgtgtt ggtcgggagt gaggtcttcg gcgttgcggc
     1081 ggagcagttt acggacggtg tagatcgcgt cgcccttgcg ggcgcggcgg ccgtgctgtt
     1141 tccaggtgag gcggcgacgc aggtcggcga ggtggcgctg ggcgagttgc acgatgtgga
     1201 aggcgtccac gacgacggtg gctttgggca gtgcggtgcg gatcgcggag cggaaggtgg
     1261 cgcacaggtc gatggcgacg tagcggacct ggtcggtcca ccaggtgggc tgggcctcca
     1321 gccaggcggc cacggagccg gcgttgcggc cctcgacctg cccgaacagg ccgtgtccgc
     1381 cgatcgcgtc gacgaagccg atgtgccagg cgtccgcctc cagcgcccac ttcccactgt
     1441 cctcgtcgag cttccagacc ggccggcccc gtcgggtctc atcgatcccg atcgcttcgc
     1501 acgcgggcgg ctcggcgggc agcacccgct cggcgaagtt gctggtggca cgcatgacga
     1561 ccggccagga ggggcccagg tcgcggccgg cctggacgac cgtgcgccca ccgtcgacca
     1621 ccgcctgccc gcacgccgtg cgcagtcgtt cggtgaggcg catccgggcc ggtatctgag
     1681 cgatctgctc ggtgaacgag ccgcgctcac aggctgtttc acggcactgc cagcgccgct
     1741 tgtgccagcg gatctgcact ccgcttccgc cgtgggacag gtcccgtggc cgggtcacgg
     1801 cccgctcctt cactcgcgtg gcgaacgttc cgcacgccgg acaggcccgc gccgccggat
     1861 ccacggtcgc cagatgcacc gtgcgcgtcc cgtcctcgcc ccggaccacc tccaccaccg
     1921 cgaccccctc cagatcgaac agcgccgtcg cgccggtatc gtgctccatg ccgcccgcgt
     1981 gtacttgaag atcgactgtc tagacaccag tcatgatcca caagtgccgc gggcggtcta
     2041 cgatcaggcc cgcaccccca cccggcagtc acgctccgca accaacgagg cggccgatgg
     2101 gtgcccctcc ccgcttaaat tcgaagaccc gccatgacgc ccggatgcaa gttacagcta
     2161 tctgtcatgc ccaaccgacc tgtcgaaata ggcccggccg gcctcc
//
LOCUS       DBNHXO010000205         5971 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3826761, whole genome shotgun sequence.
ACCESSION   DBNHXO010000205 DBNHXO010000000
VERSION     DBNHXO010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5971)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3826761"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..3461
                     /locus_tag="AC162A_04385"
     CDS             81..3461
                     /locus_tag="AC162A_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5010110.1"
                     /translation="MDERGQQGEAVARPRPGGLGRAAKLVPPRDPNGMLERAALEARV
                     AAGTDRRLTIIVAGAGFGKSTLASRAARERRAAWYTVDAADVHLGTFAAGVVAALHRQ
                     LDALPEDLATPIASAVDPRDDTEAQHRGQAAATLVADALQDVLVDELLLVLDDCHEID
                     GATGSWRFLEALVRFAPSNLHVLLVSRNEPPFGVERLRAQGEVSDLGGSALAFTVPEI
                     EALVESLLPADAVPRDATSIAATRILTATDGWPAAVRLTIEALRAAPAGGREAVLERL
                     QRPEGPLFPYLAEEVVAHASASIRYALARAAHFDRFSGPLLEAVGIDDATQVLDGLAR
                     RGLFLQPLPGDPGWYVLHGLIREYAKSRLALTGGETSELHLRAAAWLEANERYDDALA
                     QLALAGSPDALAGFLDRHGPMLVLAGSTRTVTEAATTIPEAMRTPRLERACGEAYMVR
                     GDWRAATAAFTRAAGGANRLDPESAWRLGLVHGLRGAYDEALEIYARAELTGDEPAEE
                     ALLFAWIASAHYHRGDVAESAEAAARSLELALRSGDARALCAAYTAQGMGAELAHDFH
                     GAARSFDRALELAGQAGDALQEVRIRNARGALELDRGDVGAAFETLDHAVRLADTVGF
                     ASFHARALVNRGRAHQGLGRFEEALADFTAAREIYDRVGSPSVAYPLVREGSLHLLRG
                     DPLLARFAFDTAISAARGAADAEALAPALVGLAQTIVADEPDRARELAAESVELGRDV
                     APLTVLLGAARVHVTLGEREAARTLAREATNVAAARQDQPGYASGMEIQAQTTSNTGE
                     AELLCNRAAEVWTAAGIPYGYARNRLILASILDGDRARAAAEDAAGVFRSLGARGPAT
                     EAAERLETLDRAGRPSLEIGALGRFRVLRDGEPIAATAWQSKKARDLLKILVSRRGRP
                     TTRETLFEVLWPDEDPEPLANRLSVALATMRAVLDPEKRFAMDWFVGGDKQAVWLDLS
                     HVDLDVEAFLAAAAEGLRLVREGASTAARRALETAESRYGGEFLEEDPYEDWAVGLRE
                     EAQAAYISVTRLLADLAAAAGDADGATRYSLRILERDAYDEAAHLGLVGALLAVGRHG
                     EARRRYGIYADRMEEMGVEAAPFPAVSGRERGRAPSMA"
     gene            complement(3492..3932)
                     /locus_tag="AC162A_04390"
     CDS             complement(3492..3932)
                     /locus_tag="AC162A_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010111.1"
                     /translation="MAADPLEAVLRLVDDGVLSAEEAAPILAALDEQDARIADDPGSE
                     AGRGSTPPGGFGANPPPGSASGEAPRTEAGGGARSLRIEVTDDGRRVVNLRLPIAVGK
                     LALDRIPGLPADQVTRVREALRTGYRGSILEVDDDGNGVRIVLE"
     gene            complement(3932..5041)
                     /locus_tag="AC162A_04395"
     CDS             complement(3932..5041)
                     /locus_tag="AC162A_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR5010112.1"
                     /translation="MTETTTPVAREVPLAPGGHVEILLTSANVSVRGVDGDRVSVRTR
                     GGEDIDEEVVVEATPQKVRISDNQAGSGAGGLGRRGRGPADLDIDVPRTARVSLRSLS
                     GDVEAFDIGGESHWASASGDLRVELDGGSVGLESMSGDIKLEASAAVEVRARSVSGDL
                     TLRAPRFERLSASSTSGDVRIAGALGDRGEHVVSSVSGDVDLTTASEVRVETKSITGD
                     VRAIGTWVTEGGRGRRTLVVGAGSIGVSIRTTSGDIRLRGEVPAPIVPPVPEIPRLPE
                     APVPPEAPEPPAAPPPVVIAEAEAAPNLVRPTTSLPDDPADTALGAAGPTDRREEARL
                     EILRALERGDLDIESASHRLEILEDAGPRYFRGWC"
     gene            complement(5041..5505)
                     /locus_tag="AC162A_04400"
     CDS             complement(5041..5505)
                     /locus_tag="AC162A_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HIR5010113.1"
                     /translation="MPHDVIATCPVCSGELSVTRLRCGDCGTVIEGEFGVGRFARLTR
                     EQTLVLESFLRSRGNLRDMERELGISYPTVRARVEALIRALGFGPRDEPDDIPSGAAA
                     DIDTTLEDAAQAAATAAETIQAGRREILEQLSRHELSAEAAAEAIRALGRTR"
     gene            5619..>5971
                     /locus_tag="AC162A_04405"
     CDS             5619..>5971
                     /locus_tag="AC162A_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HIR5010114.1"
                     /translation="MRFTTTIQLEGKTATGFQVPPEVVEALGRGKRPPVSVTINGYTY
                     RSTVAAYGEIYLLPLAAEHRDGAGVAAGDVIAVDLQRDEAPREVEVPADLAAALADEP
                     EARAFFEGLSYSNPR"
BASE COUNT          738 a         2238 c         2201 g          794 t
ORIGIN      
        1 gcgcgcgcgt gcagtggcgc gcggtcgtga ggtcccgagc ggggccgtta ccatgggccc
       61 tcgacgccag cggagggcca gtggacgagc ggggacagca gggcgaggcc gtcgcacgcc
      121 cacgcccggg cggcctgggc cgcgccgcca agcttgtccc gccccgtgac cccaacggca
      181 tgctcgagcg cgccgcgctc gaggcgaggg tcgccgccgg gaccgaccgg cggctcacga
      241 tcatcgtcgc cggggccggc ttcggcaaga gcacgctcgc gtcccgcgcg gcccgcgagc
      301 gtcgggcggc ctggtacacc gtcgacgccg ccgacgtcca cctcggcacg ttcgcggccg
      361 gcgtcgtggc cgcgctccac cgccagctcg acgcgctccc cgaggacctc gcgacgccga
      421 tcgcctccgc ggtcgacccg cgcgacgaca ctgaggcgca gcatcgcggc caggctgcgg
      481 cgacgctcgt cgccgacgcg ctccaggacg tcctcgtcga cgagctcctc ctggtcctcg
      541 acgactgcca cgagatcgat ggcgccaccg gctcgtggcg gtttctggag gcgctcgtcc
      601 ggttcgcccc ctcgaacctt cacgtgctgc tcgtctcgcg caacgaaccg ccgttcggcg
      661 tcgagcggct gcgcgcccag ggggaggtat cggacctcgg cggctcggcc ctcgcgttca
      721 cggtccccga gatcgaggcg ctcgtcgaga gcctcctgcc ggccgacgcc gtcccccgcg
      781 atgcgacctc gatcgccgca acccgcatcc tcaccgcgac cgatggctgg cccgcggccg
      841 tccggctgac gatcgaggcg ctccgcgcgg cgcccgcggg tggccgggaa gcggtcctcg
      901 agcggctcca gcgccccgag ggaccgctgt tcccctacct cgccgaggag gtcgtcgccc
      961 acgcgtcggc gtcgatccgg tacgcgctcg cccgcgctgc ccatttcgac cggttctcgg
     1021 gtcccctcct cgaggccgtc ggcatcgacg acgcgaccca ggtcctcgac ggcctcgccc
     1081 ggcgcgggct gttcctccag ccgctccccg gtgaccccgg ctggtacgtc ctccacggtc
     1141 tgatccgcga gtacgcgaag tcgcggctgg cgctgaccgg cggcgagacg tcggagctgc
     1201 acctcagggc ggccgcgtgg ctcgaagcca acgagcgata cgacgatgcg ctcgcgcagc
     1261 tggcgctggc ggggtcgccc gacgcgctgg ccgggttcct cgaccggcac ggaccgatgc
     1321 tcgtgctcgc cgggtcgacg cgcacggtca ccgaggcggc gaccaccatc cccgaggcca
     1381 tgcgcacacc gcgcctcgag cgcgcctgcg gcgaggcgta catggttcgt ggcgactggc
     1441 gcgcggccac cgcggcattc acccgcgcgg ccggcggtgc caaccggctc gatcccgagt
     1501 cggcctggcg gctgggtctc gtccacggcc tgcgcggcgc ctatgacgag gcgctggaga
     1561 tctacgcccg ggcggagctg acgggcgacg aaccggccga ggaggcgctg ctgttcgcct
     1621 ggatcgcctc cgcccactac caccggggcg acgtcgcgga gagcgcggag gcagccgccc
     1681 ggtcgctcga gctggcgctc cggtccggcg atgcccgcgc cctgtgcgcc gcgtacacgg
     1741 cacagggcat gggcgcggag ctggcccacg acttccacgg ggccgcccgg tcgttcgacc
     1801 gggccctcga gctggcaggg caggccggag acgccctcca ggaggtgcgc atccggaacg
     1861 cgcgcggtgc cctggagctc gaccgcggcg acgtcggggc tgcgttcgag accctcgacc
     1921 acgccgtgcg cctcgccgac acggtcggct tcgcctcgtt ccacgcccgg gcgctcgtca
     1981 atcgcggacg cgcgcaccag ggcctcggcc ggttcgagga ggcgctcgcg gacttcaccg
     2041 cggctcgcga gatctacgac cgtgtcggct cgccgtcggt cgcctatccg ctcgtccgcg
     2101 agggatcgct ccacctgttg cgcggcgatc cgctgctcgc ccggttcgcc ttcgacacgg
     2161 cgatcagcgc tgcccggggc gccgccgatg ccgaggccct ggccccggcc ctggtcggcc
     2221 tcgcccagac catcgtcgcc gacgagcccg accgcgcccg ggagctggct gcggagtccg
     2281 tcgagctcgg ccgcgacgtc gcgccgctca cggtgctcct gggggccgcc cgggtccacg
     2341 tcacgctcgg cgagcgcgaa gcggcgcgaa ccctcgcccg cgaggcgacc aacgtggcgg
     2401 ccgcgcgcca ggaccagccc gggtatgcgt cggggatgga gatccaggcg cagacgacgt
     2461 cgaacacggg cgaggcggag ctgctgtgca accgggcggc cgaggtatgg acggcggcgg
     2521 ggatcccgta cggctacgcg cgcaatcggc tgatcctcgc gtcgatcctc gacggcgatc
     2581 gcgcgcgcgc cgcggccgag gatgccgccg gggtcttccg ctcgctcggc gcgcgcggcc
     2641 cggccaccga ggccgccgag cggctcgaaa cgctcgaccg ggccggccgg ccttcgctcg
     2701 agatcggcgc actcgggcgc ttccgggtgc tccgggacgg cgagccgatc gccgcgacgg
     2761 cgtggcagtc gaagaaggca cgcgacctgc tcaagatcct cgtctcgcgg cgcggccgcc
     2821 cgaccacgcg cgagacgctg ttcgaggtcc tgtggcccga cgaggacccg gagccgctgg
     2881 ccaaccgcct ctcggtcgcc ctggcgacga tgcgcgccgt gctcgatccg gagaagcggt
     2941 tcgcgatgga ctggttcgtg ggcggcgaca agcaggccgt ctggctcgac ctctcccacg
     3001 tcgacctcga cgtcgaggcg ttcctcgccg cggccgccga gggcctgcgc ctggtccgcg
     3061 aaggggcttc gaccgcggcg cgacgggcgc tcgagacggc cgagagccgc tacggcggcg
     3121 agttcctcga ggaggatccc tacgaggact gggcggtcgg actgcgcgag gaggcgcagg
     3181 cggcctacat ctcggtgacc cggctgctcg cggacctcgc ggccgccgcg ggcgacgccg
     3241 acggcgcgac ccgctactcg ctgcggatcc tggagcgcga cgcctacgac gaggcggccc
     3301 acctgggtct ggtcggggcg ctgctcgctg tcggccgcca cggtgaagca cgccggcgct
     3361 acggcatcta cgccgaccgg atggaggaga tgggcgtcga ggccgcgccg ttccccgcgg
     3421 tcagtggccg ggaacggggt cgggcaccct cgatggcctg agggtgcgga cgcgcccgat
     3481 ggcgagctct gtcactcgag gacgatccgg acgccgttgc cgtcgtcgtc gacctcgagg
     3541 atcgacccgc gatagccggt ccggagcgcc tcgcggaccc gcgtgacctg atcggccggg
     3601 agaccgggga tacggtcgag ggcgagcttg ccgacggcga tggggagccg gaggtttacc
     3661 acgcgacggc cgtcgtcggt gacctcgatc cgcagcgaac gggcgccgcc acccgcctcc
     3721 gttcggggcg cttcgcccga cgcggatccc ggcggcgggt tggccccaaa gccgcccggc
     3781 ggggtcgatc cgcggccggc ctcgctgcct ggatcgtcgg cgatccgcgc gtcctgctcg
     3841 tcgagcgcgg caaggatcgg ggcggcctcc tcggccgaca gcacgccgtc gtcgacgagc
     3901 cgcaggaccg cctcgagcgg gtcggcggcc atcagcacca cccccggaag tagcgcggac
     3961 cggcgtcctc gaggatctcg agccggtgcg acgccgactc gatgtcgagg tcgcctcgct
     4021 cgagggcccg caggatctcg agccgggcct cctcgcgacg gtcggtgggg ccggccgcgc
     4081 cgagcgcggt gtcggccggg tcgtcgggaa gcgacgtggt cgggcggacg aggttgggtg
     4141 cggcctcggc ctcggcgatg acgacgggcg gcggggcggc cggcggctcg ggggcctcgg
     4201 ggggaaccgg cgcctccggg agccgcggaa tctcgggaac ggggggcacg atcggtgccg
     4261 gcacctcgcc gcgcagccgg atgtcgccgg acgtcgtgcg gatcgacacg ccgatcgacc
     4321 ctgccccgac gaccagcgtg cgtcggccgc ggccgccctc ggtgacccag gtgccgatgg
     4381 cgcgaacgtc gccggtgatc gacttggtct cgacccggac ctcgctcgcc gtcgtcaggt
     4441 cgacgtcgcc gctgaccgag ctgaccacgt gctcgccgcg atcgccgagc gcgccggcga
     4501 tccggacgtc gccgctggtg ctggacgcgg acagccgctc gaacctcggc gcgcggagcg
     4561 tgaggtcacc cgagaccgac cgggcacgga cctcgaccgc cgcggaggct tcgagcttga
     4621 tgtcacccga catcgactcg aggccgaccg accccccgtc gagctcgacc cgcaggtcac
     4681 ccgacgccga cgcccagtgg ctctcgccac cgatgtcgaa cgcctcgacg tcgccgctca
     4741 gggagcgcag gctgacgcgc gccgttctgg gcacgtcgat gtccaggtcg gctgggccgc
     4801 gtccgcggcg tccgaggccg cccgcccccg agcccgcctg gttgtcgctg atgcgcacct
     4861 tctgcggggt ggcctcgacg accacctctt cgtcgatgtc ctcgccgccg cgggtcctga
     4921 ccgacacccg gtcgccgtcg acgccgcgga cgctgacgtt cgcgctggtg agcaggatct
     4981 cgacgtggcc gccgggcgcg agcgggacct cgcgagccac gggtgttgtg gtctcggtca
     5041 tcatcgggtc ctccccaggg cgcggatggc ctcggcggcc gcctcggcgc tcagctcatg
     5101 gcgggacagc tgctcgagga tctcgcgccg tcccgcctgg atggtctcgg ccgccgtggc
     5161 cgcggcctgc gccgcgtcct cgagcgtcgt gtcgatgtcg gctgcggcac ccgacgggat
     5221 gtcgtccggc tcgtcgcgcg ggccgaagcc cagggcgcga atgagcgcct ccacccgggc
     5281 gcgaacggtg ggatagctga tgcccagctc ccgctccatg tcgcgcaggt tgccgcggct
     5341 gcgcaggaag ctctccagca ccagggtctg ctcgcgggtc aggcgcgcaa agcggccgac
     5401 cccgaactcg ccctcgatca cggtcccaca gtcgccgcac cggaggcgcg tgacgctgag
     5461 ctcgcccgaa cagaccgggc aggtggcgat gacatcgtgc ggcactggct tcctcgtcag
     5521 atgtgaatgc gtctttctct tttctaatac gcgctttccc tattgtcaag tcgagcgagt
     5581 acttcgtgcg agaccagctc gccgttacac tgtgcgccat gcggttcacg acgacgatcc
     5641 agctcgaggg caagacggcc acggggttcc aggtgccgcc cgaggtcgtg gaggcgctcg
     5701 gccgcggcaa gcgtccgccg gtcagcgtca cgatcaatgg ctacacctac cggagcacgg
     5761 tcgcggcgta cggggagatc tacctgctcc cgctggcggc cgagcaccgc gacggggccg
     5821 gggtcgccgc cggcgacgtg atcgcggttg acctccagcg ggacgaggcg ccgcgtgagg
     5881 tcgaggtgcc ggcggacctg gcggcggcac tggccgacga gcccgaggcg cgcgccttct
     5941 tcgagggcct ctcatacagc aacccgcgct g
//
LOCUS       DBNHXO010000206         1970 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2635125, whole genome shotgun sequence.
ACCESSION   DBNHXO010000206 DBNHXO010000000
VERSION     DBNHXO010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2635125"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..958
                     /locus_tag="AC162A_04410"
     CDS             86..958
                     /locus_tag="AC162A_04410"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR5010115.1"
                     /translation="MTDRLDPPPLAEPAAPASGRRRATARLRRAVPFLAGVLATFLAI
                     ALYLALFPAKPGITQRQVADTVASALASQTPGPARSELVFAAIQPSLVLIEIDTAPDE
                     TPKPGAATPPTEGLGSGVVVNLAGQILTALHVVEDATHIKVTFADGSSTGATISSRDE
                     DSDIAVLDPDEIPAQLVPATLGNPAAMRIGSEAYIVGNPYGLYGSLSSGVVSGLERSF
                     RAPDSDRVYTGLIQVDAAVNPGNSGGPLLDRNGNVTGIVTALINPTDDDVFIGIGLAV
                     PIEVAGGAAGAPQY"
     gene            1015..>1970
                     /locus_tag="AC162A_04415"
     CDS             1015..>1970
                     /locus_tag="AC162A_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5010116.1"
                     /translation="MEKLLYEVKKVIVGQDHLLERMIVALLARGHLLVEGVPGLAKTM
                     AVKTLAQATGGEFKRIQFTPDLVPSDLVGTRIYNQKTGEFTTSLGPVFTNLLLADEIN
                     RAPAKVQSALLEVMQERQVTIGHDTHAVPDPFLVLATQNPIETEGTYALPEAQVDRFM
                     LKVIIGYPTPTEEFAIVERMTGRLDGIAQVLSAGELIALQQVVDRVYVDPALYEYAVR
                     LSSATRTPRAYGIPEIERYITYGASPRASINLILTARALAYVRGREYVLPQDVLDMAP
                     DVMRHRLVLSFEALSDDVSPDAILATILGRVTPPVVPLQDHAP"
BASE COUNT          298 a          758 c          637 g          277 t
ORIGIN      
        1 ggttcgcggt ccaccatcgg ggcggcatcc atgtgcggga tccgttcctg cacaccggat
       61 cgttccacgg agtccgtgct tgccgatgac cgaccggctg gacccgccgc ccctcgccga
      121 accggccgcg cccgcaagcg ggcgtcggcg ggcgaccgcc cggctccgtc gtgccgttcc
      181 gttcctcgcg ggcgtcctcg ccacgttcct ggccatcgct ctatatctcg ccctgttccc
      241 ggccaagccc ggcatcaccc agcgccaggt cgcggacacc gtcgccagcg ccctcgcctc
      301 gcagacgccc ggcccggccc gctccgagct cgtgttcgcc gcgatccagc cgtcgctcgt
      361 gctgatcgag atcgacaccg cgcccgacga gacgcccaag cccggcgcgg ccacgccgcc
      421 caccgagggc ctcggcagcg gggtcgtggt caacctggcc ggccagatcc tcacggcgct
      481 ccacgtggtc gaggacgcca cccacatcaa ggtcacgttc gccgacggct catcgaccgg
      541 cgcgacgatc tcgagtcgcg acgaggacag cgacatcgcg gtgctcgacc ccgacgagat
      601 ccccgcccag ctcgtgcccg cgaccctggg caatcccgcc gccatgcgca tcggcagcga
      661 ggcctacatc gtcggcaacc cgtacggcct gtatgggtcg ctgagctcgg gcgtcgtgtc
      721 cgggctggaa cgctcgttcc gggcgcccga ctcggaccgc gtatacaccg ggctcatcca
      781 ggtcgacgcc gccgtcaatc ccggcaactc cggggggccc ctgctcgacc gcaacggcaa
      841 cgtgaccggc atcgtgaccg ccctgatcaa cccgaccgac gacgacgtgt tcatcggcat
      901 cgggctcgcc gtgcccatcg aggtcgccgg cggtgccgcc ggcgccccgc agtactgagc
      961 caggaggagg tgacgcccgt ggatcgcgac cccaacgccc gacccgccgc tccgatggag
     1021 aagctgctgt acgaggtcaa gaaggtcatc gtcggccagg accacctgct cgagcggatg
     1081 atcgtggcgc tgctggcgcg gggccacctg ctggtcgagg gcgtgccggg cctggccaag
     1141 acgatggcgg tcaagacgct cgcccaggcc acgggcgggg agttcaagcg gatccagttc
     1201 acgcccgacc tcgtcccgtc cgacctggtc gggacgcgga tctacaacca gaagaccggc
     1261 gaattcacga cctcgctggg acccgtgttc acgaacctgc tgctggccga cgagatcaac
     1321 cgggcgccgg cgaaggtcca gagcgccctg ctcgaggtga tgcaggagcg gcaggtcacg
     1381 atcggccacg acacgcacgc cgtccccgac ccgttcctgg tgctggcgac gcagaacccg
     1441 atcgagaccg aggggacgta cgcgctgccc gaggcgcagg tcgaccggtt catgctcaag
     1501 gtgatcatcg gctatccgac cccgaccgag gagttcgcga tcgtcgagcg gatgacgggg
     1561 cggctcgacg gcatcgcgca ggtcctctcg gcaggcgagc tcatcgcgct gcagcaggtg
     1621 gtcgaccgcg tctacgtcga ccccgcgctg tacgagtacg cggtccgcct gtcgagcgcg
     1681 acgcggacgc cgcgcgccta cggcatcccc gagatcgagc gctacatcac ctacggcgcc
     1741 agcccgcgcg catcgatcaa cctgatcctc acggcacgcg ccctggcgta cgtccgggga
     1801 cgcgagtacg tgctccccca ggacgtcctc gacatggcgc ccgacgtgat gcgccaccgg
     1861 ctcgtcctct cgttcgaggc cctctcggac gacgtctcgc cggacgcgat cctcgccacg
     1921 atcctcggcc gcgtcacgcc gccggtcgtc cccctccagg accatgctcc
//
LOCUS       DBNHXO010000207         3342 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3230621, whole genome shotgun sequence.
ACCESSION   DBNHXO010000207 DBNHXO010000000
VERSION     DBNHXO010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3230621"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /gene="rplD"
                     /locus_tag="AC162A_04420"
     CDS             <1..510
                     /gene="rplD"
                     /locus_tag="AC162A_04420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR5010117.1"
                     /translation="RHLGTHDTRTRGEVRGGGRKPYRQKGTGRARQGSRTAPHYRGGG
                     VVFGPHPRSYDQKLPRKMRRIALRSALTAKFGDDAIRVVDRFGMDDEPRTKAFVAVLE
                     ALGAGDGHHRRVLVVAPAADALLLRSASNLPSVIVIRADSLNVVDLLNADAVLIEQPA
                     LAKMEEVYA"
     gene            516..821
                     /gene="rplW"
                     /locus_tag="AC162A_04425"
     CDS             516..821
                     /gene="rplW"
                     /locus_tag="AC162A_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR5010118.1"
                     /translation="MTAADIILRPVISEKSIDESGRGKYTFAVHSNANKIQIKAAVEE
                     LYKKEKVTVVAVNVLTSKAKEKRRGTKRGRIVGHTSSWRKAIVTLAPGQKIEFFEGV"
     gene            824..1654
                     /gene="rplB"
                     /locus_tag="AC162A_04430"
     CDS             824..1654
                     /gene="rplB"
                     /locus_tag="AC162A_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR5010119.1"
                     /translation="MPLRSYKATSAGRRWMTRSTFEEITTDKPHKPLLEPQKRGSGRN
                     NQGRLTVRHRGGGEKTHYRIIDFKRDKPGVPAKLATIEYDPNRSARIGLLHYRDGAKR
                     YMLLPNGLKVGDVVVSGPDAEARLGNALPIAKIPLGTTIHNIELVPGKGGQIVRSAGV
                     SAQLLAKEGDYAQVRLPSGEVRRVSIRCMATIGQVSNVDHENQSLGKAGRARHMGQRP
                     EVRGVAMTPRDHPHGGGEGKSPTGMPPKTPWGKPAMGYRTRRGKATSRVIVRSRHRKS
                     "
     gene            1669..1953
                     /gene="rpsS"
                     /locus_tag="AC162A_04435"
     CDS             1669..1953
                     /gene="rpsS"
                     /locus_tag="AC162A_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR5010120.1"
                     /translation="MSRSVKKGPFVQPRLLGRVQDMNKRNERKVLKTWSRASVIFPDF
                     IGHTIAVYNGRKHVPVYVTENMVGHRLGEFAPTRTYRGHGRTADRSAGLR"
     gene            1964..2302
                     /gene="rplV"
                     /locus_tag="AC162A_04440"
     CDS             1964..2302
                     /gene="rplV"
                     /locus_tag="AC162A_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR5010121.1"
                     /translation="MRVSATAKYLRGSTRKARLITEAIKGMPVEDAAAALRFMPQAAA
                     RDVARVLKSATANAENNLSRSADELYIADAVANEGPTIKRWRPRAQGRAFPIHKPMTH
                     ITITVADREA"
     gene            2305..2997
                     /gene="rpsC"
                     /locus_tag="AC162A_04445"
     CDS             2305..2997
                     /gene="rpsC"
                     /locus_tag="AC162A_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR5010122.1"
                     /translation="MGHKVHPYGFRLGYTRSWTAKWYADKEYTTLLKEDIAIRQLLDR
                     RLANASVSGVEIERGINHVTVAIHTAKPGIVIGRGGQNVEILRKEIGELTKRKVKLEI
                     REIRQPELDAYLVALNIAQQLTRRVAYKKAMKQSIQRSMKAGAKGVRIALAGRLGGSE
                     MGRREWDREGRIPLGTLRADISYGQVHAHTTYGRIGVKVWIYRGEIPVERHRREQAAA
                     AVGAAAAAGQGG"
     gene            3002..>3342
                     /gene="rplP"
                     /locus_tag="AC162A_04450"
     CDS             3002..>3342
                     /gene="rplP"
                     /locus_tag="AC162A_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR5010123.1"
                     /translation="MLMPRRVKHRKVMRGRMSGMAKGGHTVAFGEYGLATLEPAWITA
                     RQIEAARRAMTRSVKRGGKIWIRVFPDKPATKKPAETRMGSGKGAPDHWVAVVRPGRI
                     LFEMAGVEHEAA"
BASE COUNT          595 a         1196 c         1135 g          416 t
ORIGIN      
        1 cgccatctcg gcacccacga cacccgcacc cgcggcgagg tccgcggcgg cggccgcaag
       61 ccgtaccgcc agaagggcac cggccgcgcg cgccagggct cccgcaccgc cccgcactac
      121 cgcggcggcg gcgtcgtgtt cggcccccac ccgcgttcct acgaccagaa gctgccgcgc
      181 aagatgcggc gcatcgcgct gcgctccgcg ctgacggcca agttcggcga cgacgccatc
      241 cgcgtcgtgg accgcttcgg catggacgac gagcccagga cgaaggcgtt cgtcgcggtg
      301 ctcgaggccc tgggcgccgg cgacggccac catcgccgcg tcctggtcgt ggccccggca
      361 gcggacgccc tgctgctccg gtcggcctcg aacctgccgt cggtgatcgt catccgcgcc
      421 gactcgctca atgttgtcga cctgctcaac gcggacgccg tcctgatcga acagccggcc
      481 ctggcgaaga tggaggaggt gtacgcgtga gcgtcctgac cgccgccgac atcatcctgc
      541 gcccggtgat ctcggagaag tcgatcgacg agtcgggccg cggcaagtac acgttcgccg
      601 tccactcgaa cgcgaacaag atccagatca aggccgccgt cgaggagctg tacaagaagg
      661 agaaggtgac cgtcgtcgcc gtcaacgtgc tcacctcgaa ggccaaggag aagcgccgcg
      721 gcaccaagcg cgggcggatc gtcggccaca cgtcttcctg gcggaaggcc atcgtgaccc
      781 tggccccagg ccagaagatc gagttcttcg agggggtgta ggaatgccgc tccgaagcta
      841 caaggcaacc tcggccggcc gccggtggat gactcgctcg acattcgagg agatcaccac
      901 cgacaagccg cacaagccgc tgctcgagcc gcagaagcgc gggtccggcc gcaacaacca
      961 gggccggctc accgtgcgcc atcgcggagg cggcgagaag acccactacc ggatcatcga
     1021 cttcaagcgt gacaagccgg gcgtccccgc caagctggcc acgatcgagt acgacccgaa
     1081 ccggtcggcc cgcatcggcc tgctccacta ccgggacggc gccaagcgct acatgctgct
     1141 gcccaacggc ctcaaggtcg gcgacgtcgt cgtctccggc ccggacgccg aggcgcgcct
     1201 cggcaacgcg ctgccgatcg ccaagatccc gctcggcacg acgatccaca acatcgagct
     1261 tgtccccggc aagggcggcc agatcgtccg gtccgcgggc gtctcggcgc agctcctggc
     1321 caaggagggc gactacgcgc aggtgcgcct cccgtcgggc gaggtccgcc gggtgagcat
     1381 ccgctgcatg gcgacgatcg ggcaggtctc gaacgtcgat cacgagaacc agagcctcgg
     1441 caaggccgga cgtgcccgcc acatgggcca gcgtccggag gtccggggcg tcgcgatgac
     1501 cccgcgggac cacccgcacg gcggtggcga gggcaagagc cccacgggca tgccccccaa
     1561 gacgccgtgg ggcaagcccg ccatgggcta ccggacgcga cgtggcaagg cgacgagccg
     1621 cgtcatcgtc cgcagccgtc atcgcaagtc gtagagagga gctagagcat gtcgcgatcg
     1681 gtcaagaagg gaccgttcgt ccagccccgg ctcctcggcc gggtccagga tatgaacaag
     1741 cggaacgagc gcaaggtctt gaagacctgg tcgcgcgcgt ccgtcatctt ccccgacttc
     1801 atcgggcaca ccatcgccgt ctacaacggc cggaagcacg tgcccgtgta cgtcaccgag
     1861 aacatggtcg gccatcgcct cggcgagttc gcgcccaccc gcacgtatcg cgggcacggc
     1921 cggaccgccg atcgatccgc cgggctgcgg taggggagca ctgatgcgcg tctccgccac
     1981 agcgaagtac ctgcggggtt ccacccggaa ggcccgcctc atcaccgagg cgatcaaggg
     2041 catgccggtt gaggatgccg ccgccgccct gcggttcatg ccccaggcgg ccgccaggga
     2101 cgtcgcgcgc gtgctcaaga gcgcgaccgc gaatgcagag aacaacctga gccgctcggc
     2161 cgatgagctg tacatcgccg acgcggtcgc aaacgagggc ccgacgatca agcggtggcg
     2221 ccccagggcc cagggccggg cgttcccgat ccacaagccc atgacgcaca tcaccatcac
     2281 cgtcgcggac cgggaggcct aggcatggga cacaaggtcc atccgtacgg cttccgcttg
     2341 ggctacacgc gatcgtggac ggccaagtgg tacgccgaca aggaatacac gaccctcctc
     2401 aaggaggaca tcgcgatccg gcagctgctg gaccggcggc tcgcgaacgc cagcgtgagc
     2461 ggcgtcgaga tcgagcgtgg catcaaccac gtgacggtcg cgatccacac ggccaagccg
     2521 ggcatcgtca tcgggcgcgg cggccagaac gtcgagatcc tgcgcaagga gatcggcgag
     2581 ctgaccaagc gcaaggtgaa gctcgagatc cgcgagatcc gccagccgga gctggacgcc
     2641 tacctcgtcg ccctcaacat cgcccagcag ctgacccgcc gcgttgccta caagaaggcg
     2701 atgaagcagt cgatccagcg gtcgatgaag gccggcgcca agggcgtccg catcgcgctc
     2761 gccggccgcc tcggcggttc ggagatgggt cgccgcgagt gggaccgcga aggtcggatc
     2821 ccgctcggca cgctgcgcgc cgacatcagc tacggccagg tgcacgcgca cacgacctac
     2881 ggccgcatcg gcgtcaaggt ctggatctac cggggcgaga tcccggtgga gcgtcaccgc
     2941 cgcgagcagg ccgccgccgc ggtcggtgcg gccgccgccg ccggccaggg gggttgacgc
     3001 aatgctgatg ccccgacgcg tcaagcaccg caaggtgatg cgtggccgga tgtccgggat
     3061 ggccaagggt ggacacaccg tcgccttcgg cgagtacggg ctcgccacgc tggagcccgc
     3121 ctggatcacg gcccgccaga tcgaggccgc ccggcgcgcg atgacccgct cggtcaagcg
     3181 cggcggcaag atctggatcc gcgtgttccc cgacaagccc gcgaccaaga agcccgccga
     3241 gacgcggatg ggttcgggca agggcgcccc cgatcactgg gtcgccgtcg tgcgacccgg
     3301 ccggatcctg ttcgagatgg ccggcgtcga gcacgaggcc gc
//
LOCUS       DBNHXO010000208         2477 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2834628, whole genome shotgun sequence.
ACCESSION   DBNHXO010000208 DBNHXO010000000
VERSION     DBNHXO010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2834628"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="AC162A_04455"
     CDS             complement(<1..1160)
                     /locus_tag="AC162A_04455"
                     /inference="COORDINATES: protein motif:HMM:NF025067.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HIR5010124.1"
                     /translation="MPRATARRILLVAIALGILVDIALPGHAAGLNAPIVMAALLAAA
                     FAIAGRAGLERFDPADVWMPVAALGLTAMAALRADPWLVEADLLVATALACGTLAALA
                     GARITRGLVPTVLNAAGAVLAAMFTGALTVVQRPWPAVTDAGPVRARLGPWLPVARGL
                     MLAVPIVLLFGLLFASADAVFASLARTALSLPVEIDVADVGQRTLIVAAVAWGAAGLL
                     ALAAGGLPGLIPGPTSPAAPPARSLGAASATDLAAGGRPVATIEAATVLYVVDALFAA
                     FVVLQLAYLFGGRDTLAVAGMTYSDYARRGFFELVAVAVLAGLLVVSLDLAVRQRSPA
                     QLAAGIALIALTGLVLASALVRLRLYQDAYGWTELRFLVLVAIGWLAVALAVT"
     gene            1258..2076
                     /locus_tag="AC162A_04460"
     CDS             1258..2076
                     /locus_tag="AC162A_04460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5010125.1"
                     /translation="MTDQEARYDTIAEGYAELWSPVHRPATLALLGEVERDVAAGART
                     LIDVGCGTGALVAEAAGRWPGVAVDGVDVSAGMLAIAERTGRALPAAVQTRIRFSQAP
                     ADRLPYPDASFDVALTSFVLQLVPSRHRALREIRRVLRPAGRLALVTWLRGGEPLIAD
                     RAYDEALVAAGLEPRESGGGDDDLDSPAEAVAVLRRAGFAEVTARAETLVHRFTPESF
                     LAFITRFDDADQFDALDEADRARLEADVLARLRALPSDGLRLVAPIVYATGRRT"
     gene            complement(2104..>2477)
                     /locus_tag="AC162A_04465"
     CDS             complement(2104..>2477)
                     /locus_tag="AC162A_04465"
                     /inference="COORDINATES: protein motif:HMM:NF026044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010126.1"
                     /translation="EELTVEVPLVGTGAAPATESGGTLVHPVSSVKVRALPANLPDSL
                     HYDLSPLVTYDITITVADIAAPEGVTIVADPADVVARVLAPRVEEEVVVAAAEEGEAG
                     EAAASEGGDAAATSDEADAEG"
BASE COUNT          345 a          894 c          958 g          280 t
ORIGIN      
        1 gtgacggcga gcgcgacagc gagccagccg atcgccacca ggacgaggaa ccggagctcc
       61 gtccagccgt aggcgtcctg gtacagccgc aggcggacga gggccgaggc gagcacaagg
      121 ccggtcaggg cgatcagggc gatcccggcg gcgagctgcg ccggcgaccg ctggcgcact
      181 gcgagatcga gactcacgac gagcagcccc gcgagcacgg cgacggccac gagctcgaag
      241 aacccgcgtc gtgcgtagtc gctgtaggtc atgccggcga ccgcgagcgt gtcccggcct
      301 ccgaacaggt acgccagctg gaggacgacg aaggccgcga acagcgcgtc gacgacgtac
      361 aggaccgtcg cggcctcgat cgtcgcgacg ggccggcccc cggcggcgag gtctgtggca
      421 ctcgcggccc cgagcgatcg ggccggcggc gccgcgggcg acgtcggtcc gggaatgagc
      481 cccggcagcc caccggcggc gagcgccagc agccccgccg cgccccacgc gaccgcggcc
      541 acgatgaggg ttcgctggcc gacgtccgcg acatcgatct ctaccggcag cgacagcgcg
      601 gtccgcgcga ggctcgcgaa caccgcgtcc gccgaggcga acagcagtcc gaacaggagg
      661 acgatcggca ccgcgagcat cagcccgcgg gcgaccggca gccaggggcc gagccgtgct
      721 cgaacaggcc ccgcgtcggt gacggccggc cacggccgtt ggaccacggt cagcgcgccc
      781 gtgaacatgg ccgcgagcac ggcaccggcg gcgttgagca cggtcggaac gaggccgcgc
      841 gtgatcctcg caccagcgag ggccgcgagg gtgccgcacg cgagcgccgt cgcgaccagc
      901 aggtcggctt ccaccagcca tggatcggcc cgcaaggcgg ccatcgccgt gagcccgagg
      961 gcggcgacgg gcatccagac atcggccggg tcgaagcgct cgaggccggc ccgcccggcg
     1021 atcgcgaacg ccgcggcgag cagcgccgcc atgacgatcg gggcattcag gccggccgcg
     1081 tggccgggca gggcgatgtc gacgaggatg ccaagggcga tggcgacgag caggatgcgg
     1141 cgtgcggtgg ctcggggcat gtgcacctcc ggggcgggcc gcgcgggttg gcggccggac
     1201 gggagacggg ccgtcccgac catgtgtggc gtacgacggc gtggcaggat gcgggcgatg
     1261 accgaccagg aggcccgcta cgacacgatt gccgaggggt atgcggagct gtggtcaccg
     1321 gtccatcggc cggccacgct ggcgctcctc ggagaggtgg agcgggacgt cgccgccggc
     1381 gcccgcacgc tcatcgacgt cggatgtggg accggggcgc tggtggccga ggccgccggt
     1441 cgctggccgg gcgttgcggt cgacggggtc gacgtctcgg ccggcatgct cgcgatcgcc
     1501 gagcgtaccg gacgtgcact gcccgcggcc gtccagaccc ggatccgctt cagccaggcg
     1561 ccggcggatc ggctccccta cccggacgcg tcgttcgacg tcgcgctgac gtcgttcgtg
     1621 ctccagctcg tgccgtcgcg ccatcgggcg ctgcgcgaga tcaggcgcgt gctccgcccg
     1681 gcgggtcgcc tcgccctcgt cacgtggctg cggggcggcg agccgctcat cgccgatcgc
     1741 gcctacgacg aggcgctggt tgccgccggg ctcgagccgc gcgagtcggg cggcggcgac
     1801 gacgatctcg actccccggc cgaggcggtc gcggtcctgc ggcgcgcggg cttcgccgag
     1861 gtaacggcgc gagccgagac gctcgtccac cggttcacgc ccgagtcgtt cctcgcgttc
     1921 atcacgcgct tcgacgacgc ggaccagttc gacgcgctgg acgaggcgga ccgagccagg
     1981 ctcgaagcgg acgtgctcgc ccgactccgg gcacttccct cggacgggct ccgcctcgtc
     2041 gccccgatcg tgtacgcgac cgggcggcgg acctagccgc cgcccgcggt cgggcgaccc
     2101 aggtcagccc tcggcgtcgg cctcgtcgct cgtggcggcg gcgtcgccac cttcggacgc
     2161 ggcggcctcg ccggcctcgc cttcctcggc ggctgcgacc acgacctcct cctcgacccg
     2221 cggcgcgagc acacgggcga cgacgtcggc ggggtcggcg acgatcgtca cgccctcggg
     2281 agcggcgata tcggcgacgg tgatcgtgat gtcgtaggtg acgaggggcg acaggtcgta
     2341 gtggagcgaa tcgggcaggt tcgccggcaa cgcgcggacc ttgaccgacg agaccgggtg
     2401 caccagcgtg ccgccggact cggtcgcggg cgcggcgccg gtcccgacga gcggcacctc
     2461 gacggtgagc tcctcgg
//
LOCUS       DBNHXO010000209         3009 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4818813, whole genome shotgun sequence.
ACCESSION   DBNHXO010000209 DBNHXO010000000
VERSION     DBNHXO010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4818813"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="AC162A_04470"
     CDS             <1..1485
                     /locus_tag="AC162A_04470"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019982.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5010127.1"
                     /translation="PAQGTAAGAAADAYVDACLTEAGVPEADLPLYSTEQAIEDLEAI
                     RQYLAIDTMDLYGESYGTQYVQTYATKYPEHIKALYLDGPVDLTLDGATYYAEASHSF
                     DDVLVATLNDCATDPKCAADYGGKDPVAAYDELAARLTAAPAEIDFPKGDGTTERRTL
                     AMTDLENAAVSYLYSPGDRQILQRALAAASRDNLIPLAHLAYTAIALNPDTLAVEPDE
                     SYSDALYYAVECQDYVYNADAPDDAARLSDYLDDARTLGVPETRLGALYYGDMPCLYW
                     PNRPAADPRPAPIVAAPYPTLIMVATADPITPVANAIRLANRLSDVHVIIETGGPHVI
                     FGWGLACPDKVVARYMTQGIKPADRITICKGDVADPYVPLARADAGAYPGALALMRSI
                     ADQVQNTDDYWSRLDDEPIPGGCDFGGVLTYRPVKAGTALDFAGCEFTDGLPLTGTGL
                     IADSGAVTMDVTLPDGALRYRMRPSGQDSVTGSYRGRKVELGAG"
     gene            complement(1496..1903)
                     /locus_tag="AC162A_04475"
     CDS             complement(1496..1903)
                     /locus_tag="AC162A_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010128.1"
                     /translation="MADRRFPIRIGARSALALRIVFGATPETSWAAVDEDTVSARFGR
                     FSFSTPLSNVARYRIEGPWAWITAIGVRRSIRGGDVSFAGSPRGGVRLDVREPVKWTL
                     WHVPAFYVGTEDLEGFAAALSAHGIPGEDARRG"
     gene            2189..>3009
                     /gene="guaA"
                     /locus_tag="AC162A_04480"
     CDS             2189..>3009
                     /gene="guaA"
                     /locus_tag="AC162A_04480"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR5010129.1"
                     /translation="MTETTEEQQPAAGSEADQNRQRGKTVHGEAANAGHRVLAPDDAE
                     VEAYLEVALDAPAPERTPQDRPPITPSAAGPATVAVLDFGSQFAQLIARRVRELNVYS
                     ELLPHDTTMAELERRGVRAVILSGGPMSVYDTDAPKADASIWSGRLPVLGICYGAQLM
                     ALELGGDVTPTTRREYGPATVQITRDDALFGGIDREQPVWMSHGDSITRLPEGFHSTA
                     QTESTPFAGLDAPERNLYGIQFHPEVVHTPRGRTVLRNFVTGIAGIEPNWTPANFV"
BASE COUNT          481 a         1143 c         1003 g          382 t
ORIGIN      
        1 ccggcccagg gaaccgcggc gggcgcggca gcggatgcct acgtcgacgc ctgcctcacc
       61 gaagcgggcg tgcccgaggc cgacctgccg ctgtactcca ccgagcaggc gatcgaggac
      121 ctcgaggcga tccgccagta cctcgccatc gacacgatgg acctctacgg cgagagctac
      181 gggacgcagt acgtgcagac gtacgcgacg aagtaccccg agcacatcaa ggcgctctat
      241 ctcgacggcc cggtggacct gacgctcgac ggcgccacct actacgcgga ggcctcgcac
      301 tcgttcgacg acgtgctggt cgcgaccctc aacgactgcg cgacggaccc gaagtgcgcg
      361 gccgactacg gcggcaagga tccggtcgcg gcgtacgacg agctggccgc ccggctgacg
      421 gccgctcccg ccgagatcga cttccccaag ggcgacggga cgaccgagcg tcggacgctc
      481 gcgatgaccg acctcgagaa cgccgccgtc tcctacctgt actcgccggg cgaccggcag
      541 atcctccagc gggctctcgc ggccgcgtca cgcgacaacc tcatccccct cgcgcacctg
      601 gcctacaccg cgatcgccct caaccccgac acgctggcgg tcgagcccga cgagagctac
      661 tcggacgcgc tctactacgc cgtcgagtgc caggactatg tctacaacgc cgacgcgcct
      721 gacgacgcgg cacgcctgtc ggactacctc gacgacgcgc ggacgctcgg cgtccccgag
      781 acgcgcctgg gtgccctgta ctacggcgac atgccctgcc tctactggcc caaccgtcct
      841 gcggccgatc cccggccggc gccgatcgtc gccgcaccgt acccgacgct gatcatggtc
      901 gcgaccgccg acccgatcac gcccgtcgcg aatgcgatcc gcctcgccaa ccgcctgtcc
      961 gacgtgcacg tgatcatcga gaccggtggc ccgcacgtga tcttcgggtg gggcctcgca
     1021 tgcccggaca aggtcgtcgc gcgctacatg acccagggca tcaagcccgc cgaccggatc
     1081 acgatctgca agggcgacgt cgcggaccca tatgtcccgc tcgccagggc cgacgccggg
     1141 gcatatcccg gcgcccttgc gctgatgcgc tcgatcgccg atcaggtcca gaacaccgac
     1201 gactactggt cgcggctgga cgacgagccg atccccggcg gttgcgactt cggcggggtg
     1261 ctgacgtacc ggccggtcaa ggccgggacc gcgctcgact tcgccggctg cgagttcacc
     1321 gacggcctgc cgctgaccgg caccggactg atcgccgaca gtggcgccgt gaccatggac
     1381 gtgacgctcc ccgacggggc gctgcgctac cggatgcggc ccagcggcca ggactcggtg
     1441 accgggtcgt accgcggccg gaaggtcgag ctgggcgcag gttgaggccg gtccgctagc
     1501 cgcgtcgggc gtcctcgccg gggatgccgt gtgccgagag ggccgccgcg aagccctcga
     1561 ggtcctccgt cccgacgtag aacgccggga cgtgccagag cgtccacttc accggctcgc
     1621 gcacgtcgag gcggacgccg ccgcggggac tgcccgcgaa cgagacgtcg ccgccgcgga
     1681 tgctccgccg cacgccgatc gccgtgatcc acgcccaggg cccctcgatg cggtagcgcg
     1741 ccacgttcga cagcggcgtc gagaacgaga agcggccgaa gcgagccgac accgtgtcct
     1801 cgtccacggc cgcccaggac gtctcggggg tcgcgccgaa gacgatccgc agcgcgagcg
     1861 cggagcgcgc cccgatccgg atgggaaagc gccgatcggc catgtgtcgc agcgtacccc
     1921 tacgtcatgt gtccggcccg attcgccacc ggcgtcgatg tggccgctcg cggccagcgc
     1981 gcatcgtggc gtcagcgccc gaacaactga agaacaagcg aagaacaacc gaagaacaac
     2041 cgaatacgag cctcctccaa cgcacgcgac cggtcaccct ctcccaacct cggggcggcc
     2101 ggtcgctttt cggtcgcgcc gggtgcctgg ggtggccccg gccggataga ctcttggcca
     2161 cgaaggcggg tcgcacacgg aggggtcgtt gaccgagacg acggaggagc agcagcccgc
     2221 ggccggttcg gaggcggacc agaatcggca gcgcggcaag acggtccacg gcgaggccgc
     2281 gaacgcgggc caccgggtcc tcgcccccga cgacgccgag gtcgaggcgt acctcgaggt
     2341 cgcgctggac gcgcccgccc ccgagcgcac cccacaggat cgccctccga tcacgcccag
     2401 tgcggccggg ccggccacgg tcgccgtcct ggacttcggc agccagttcg cgcagctgat
     2461 cgcgcgccgc gtccgcgagc tcaacgtcta ctccgagctg ttgccccacg acacgacgat
     2521 ggccgagctg gagcggcgcg gcgtccgtgc ggtcatcctc tccggcggcc cgatgtccgt
     2581 gtacgacacc gacgcgccca aggccgacgc gtcgatctgg agcgggcgcc tgccggtgct
     2641 cggcatctgc tacggcgctc agctgatggc gctcgagctg ggcggggacg tgacgccgac
     2701 caccaggcgc gagtacggac ccgcgaccgt ccagatcacc cgcgacgacg cgctgttcgg
     2761 cggcatcgac cgcgagcagc ccgtgtggat gagccacggc gactcgatca cccgcttgcc
     2821 cgagggcttc cactcgacgg cccagaccga gtccaccccg ttcgccggcc tcgacgcccc
     2881 ggagcgcaac ctgtacggga tccagttcca tcccgaggtc gtgcacacgc cgcggggccg
     2941 cacggtgctg cgcaacttcg tcaccggcat cgccggcatc gagcccaact ggacgcccgc
     3001 caacttcgt
//
LOCUS       DBNHXO010000210         3742 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3231040, whole genome shotgun sequence.
ACCESSION   DBNHXO010000210 DBNHXO010000000
VERSION     DBNHXO010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3231040"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="AC162A_04485"
     CDS             <1..364
                     /locus_tag="AC162A_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR5010130.1"
                     /translation="MRRTRAGGDARLHDRWSIGVGGHLNPGDRDVLGGLRREWAEEIA
                     ADFVPDFMPVGLLNDDSTPVGAVHIGIVFVADAAGRKVAIRETDKLEGSFATTAEVAE
                     VVEGMETWSRLVFEALRD"
     gene            413..1771
                     /locus_tag="AC162A_04490"
     CDS             413..1771
                     /locus_tag="AC162A_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012783.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR5010131.1"
                     /translation="MNDRSRSLVRRIARTIVLLGVVVTGTAGIVRAADGDVVVLTATG
                     VVDNVMANYLGDGILQAAASGAPAVIVKLNTPGGSLDATQKITSALLEAPIPAIVWIA
                     PSGGRAASAGTFITMAANLAYMAPGTNIGAASPVGSNGEDILGTLGEKVKNDAVKNMT
                     AIAEARGRPVEWAVSTVEDAASYSASEAVAAGAVDGIAATVDEVLAQANGQEVTVGGV
                     TSTLDLTGVGVQETGLNPLQGLLHLLSDPNIAFVLFTIGVYGLIFELQNPNFVTGILG
                     AIAIILAFIGFGSLPLNFAGLLLLVLGVVLFVLEATVTSHGLLAIGGIICFALGASAL
                     YTNTGDPTEPIVSVALPVIFVSTATTAVLMGLITIAAIRTRNMAPPSGTVGVPVPLGT
                     EGVVQAPLEPLGTAYLAGEAWSARSTDTTTVPRDTPVRLVGFDGLTAIVEPTGEAGSA
                     AH"
     gene            1838..2764
                     /locus_tag="AC162A_04495"
     CDS             1838..2764
                     /locus_tag="AC162A_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018194581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR5010132.1"
                     /translation="MELVAVLAAVAFFGVFVLIILYLSVRVVQQYEQMVVFRLGKTNE
                     GLVRGPGLRFLIPIIDRPVKIDVREQFIEVPSQTTITRDNAPINVDFLIYWRIVDPLK
                     SIVNVAYFAGALQGVATTTLRAVIGDISLDDVLSRRDQINEVLRTKLDESTETWGGKV
                     TRVEIREITPPRDVQDAMNRQLSAERTRRAVITESEGLRQSAINVAEGQKQSEILKAE
                     GERQAAILRAEGFSQALERIYSAAHIIDEKTMALQYLEALKSLGASPSTKFVIPTEFT
                     RFAERVGDYVEAGIGHVPGPAGGEALPGPSGA"
     gene            2895..>3742
                     /locus_tag="AC162A_04500"
     CDS             2895..>3742
                     /locus_tag="AC162A_04500"
                     /inference="COORDINATES: protein motif:HMM:NF025001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5010133.1"
                     /translation="MAGKVLVVDDDASVQRLLQYTLKQEGYDVAIAADSTEGIRLWGS
                     DSPALILLDVTLQPLDGYAVAMQIRAEESAGSHVPIIMLTSERDVQQKVRALRAGADD
                     YLIKPFHPAELLARMKSLIARFAPSDSVVGRPPMGRIHAYYGAKGGVGTTTIAINAAI
                     ALQSLGRRVCLVDGNLQFGDHRVFLDLGLDRKSIVDVVSAPSMDSDLVRRVVQTHDSG
                     VDLLLAPPSPESADLVSPAHMHQVLELLSGMYDYVVVDIDKRLDDVNLMVLDVAETIF
                     AVMTADL"
BASE COUNT          576 a         1316 c         1303 g          547 t
ORIGIN      
        1 gatgcggcgg acgcgggccg gcggcgacgc gcggctccac gaccgctggt cgatcggcgt
       61 cggcggccac ctcaaccccg gcgaccgcga cgtcctgggc gggctgcgcc gcgagtgggc
      121 cgaggagatc gccgccgact tcgtgcccga cttcatgccc gtcggcctgc tcaacgacga
      181 ctcgacgccg gtcggcgccg tgcacatcgg catcgtgttc gtcgccgacg cggccggtcg
      241 gaaggtcgcg atccgggaga cggacaagct ggagggctcg ttcgccacca cggccgaggt
      301 cgcggaggtg gtcgagggga tggagacctg gagccgcctc gtgttcgagg cgctgcgcga
      361 ctgaccgacc gcgcgaccag tcgcccgccc ggtgccatac tcgccgtggg ccatgaacga
      421 ccgctcacgc tccctcgtcc gcaggatcgc ccgcacgatc gtgctgctgg gcgtggtcgt
      481 gacgggcacg gcgggcatcg ttcgcgcggc cgacggcgac gtcgtggtgc tgacggcgac
      541 cggggtcgtc gacaacgtca tggccaacta cctcggcgac gggatcctgc aggcggccgc
      601 gtcgggcgcg ccggcggtga tcgtgaagct caacacaccg ggcggcagcc tcgacgcgac
      661 ccagaagatc accagcgccc tgctcgaggc tccgatcccg gcgatcgtgt ggatcgcgcc
      721 cagcggcggc cgggcggcga gcgccgggac gttcatcacg atggcggcca acctcgccta
      781 catggcaccg ggtacgaaca tcggcgccgc gtcgccggtc ggcagcaacg gggaggacat
      841 cctgggtacg ctcggcgaga aggtgaagaa cgacgcggtc aagaacatga ccgcgatcgc
      901 cgaggcgcgc ggacgcccgg tcgagtgggc cgtgtcgacg gtcgaagacg cggcgtccta
      961 ctccgcgtcg gaggcggtgg cagcgggggc ggtcgacggg atcgccgcca cggtcgacga
     1021 ggtgctggcg caggccaacg ggcaggaggt gacggtcggc ggcgtcacca gcacactcga
     1081 cctcacgggc gtcggggtgc aggaaaccgg cctgaatccg ctgcaggggc tgctgcacct
     1141 gctgtccgac cccaacatcg cgttcgtgct gttcacgatc ggggtctacg gcctgatctt
     1201 cgagcttcag aaccccaact tcgtgaccgg gatcctgggc gcgatcgcga tcatcctcgc
     1261 gttcatcggg ttcggcagtc tgccgctcaa cttcgccgga ctgctgctgc tggtgctcgg
     1321 agtcgtcctg ttcgtgctcg aggcgaccgt caccagccac gggctgctcg cgatcggcgg
     1381 gatcatctgc ttcgcgctcg gcgcctccgc gctgtacacc aacacgggcg acccgacgga
     1441 acccatcgtg tcggtggccc tgccggtgat cttcgtgagc acggcgacga ccgcggtcct
     1501 gatgggcctg atcacgatcg cggcgatccg gacccgcaac atggcgcccc catcggggac
     1561 cgtgggcgtc ccggttccgc tcggcaccga gggcgtcgtc caggcgccgc tcgagcccct
     1621 gggcacggcc tacctcgccg gcgaggcgtg gagcgcccgg agcaccgaca ccaccaccgt
     1681 gccccgcgac acccccgtcc gcctcgtggg cttcgacggc ctcaccgcca tcgtcgaacc
     1741 gaccggggag gccgggtcgg ccgcacactg atcgccagaa cgcctaccgt atagagggcg
     1801 tccgtccggc gccgtccggc ggtcgcagga ggaattcatg gagctcgtcg ccgtgctcgc
     1861 agccgtcgcg ttcttcggcg tcttcgtgct catcatcctg tatctgtccg tccgcgtggt
     1921 ccagcagtac gagcagatgg tggtgttccg gctgggcaag acgaacgagg gcctggtccg
     1981 cggcccgggc ctgcggttcc tgatcccgat catcgatcgt ccggtgaaga tcgacgtccg
     2041 cgagcagttc atcgaggttc ccagccagac cacgatcacc cgcgacaacg ccccgatcaa
     2101 cgtcgacttc ctgatctact ggcgcatcgt cgacccgctc aagagcatcg tgaacgtcgc
     2161 ctacttcgcc ggtgcgctgc agggcgtcgc gaccaccacc ctgcgcgcgg tgatcggcga
     2221 catcagcctc gacgacgtgc tgtcccggcg cgaccagatc aacgaggttc tgcggaccaa
     2281 gctcgacgag agcaccgaga cctggggcgg caaggtgacc cgcgtcgaga tccgcgagat
     2341 cacgccgccg cgcgatgtgc aggacgcgat gaaccgccag ctctcggccg agcgcacgcg
     2401 gcgcgcggtc atcacggagt ccgagggcct gcggcagtcg gcgatcaacg tcgccgaggg
     2461 ccagaagcag tccgagatcc tcaaggccga gggcgagcgc caggcggcga tcctgcgggc
     2521 cgagggcttc agccaggcgc tcgagcggat ctactcggcc gcccacatca tcgacgagaa
     2581 gacgatggcc ctccagtacc tcgaggcgct gaaatcgctc ggcgcgagcc cgtcgaccaa
     2641 gttcgtgatc ccgaccgagt tcacccggtt cgccgagcgg gtgggcgact acgtcgaagc
     2701 cggcatcggc cacgtgcccg gtcccgccgg tggcgaggcg ctgccgggcc ccagcggggc
     2761 ctgatcgctg gaaccggggt tgggggcccg cgtcgtgtgg agcgcccgcc cgcgtcccta
     2821 tactggctgc cagcaccggc ggtgatgccg cgcatcgctc gccgcgatcg ggagcctcgt
     2881 gccaggagaa cggattggcc gggaaagtcc tcgtcgtcga cgacgatgcg agcgtccagc
     2941 gcctgctgca gtacaccctc aagcaggagg ggtacgacgt ggcgatcgcc gcggacagca
     3001 ccgaagggat tcgactgtgg gggtcggact cgccggcgct gatcctgctc gatgtcacgc
     3061 tgcagccgct cgacggatat gccgtggcga tgcagatccg cgccgaggag agcgccggca
     3121 gccacgtgcc gatcatcatg ctcacgtccg agcgggacgt gcagcagaag gttcgggcgc
     3181 tgcgcgctgg agccgatgac tacctgatca agcccttcca cccggccgag ctgctcgcgc
     3241 ggatgaagag cctgatcgcc cgcttcgcgc ccagcgactc cgtggtcgga cggccgccga
     3301 tgggccggat ccacgcctac tacggcgcga agggcggcgt cgggacgacg acgatcgcga
     3361 tcaacgcggc gatcgccctc cagagcctcg gccggcgcgt gtgcctcgtc gacggcaacc
     3421 tccagttcgg ggaccaccgc gtgttcctcg acctcggcct cgaccgcaag agcatcgtcg
     3481 acgtcgtcag cgcgccgtcc atggactcgg acctggtccg gcgggtcgtc cagacgcacg
     3541 actcgggcgt cgacctgctg ctcgccccgc cgtcgcccga atcggccgac ctcgtgtcgc
     3601 ccgcccacat gcaccaggtg ctcgagctgc tgagcgggat gtacgactac gtcgtcgtcg
     3661 acatcgacaa gcggctcgac gacgtcaacc tcatggtgct cgacgtcgcg gagacgatct
     3721 tcgcggtcat gacggccgac ct
//
LOCUS       DBNHXO010000211         2257 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3628293, whole genome shotgun sequence.
ACCESSION   DBNHXO010000211 DBNHXO010000000
VERSION     DBNHXO010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3628293"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="AC162A_04505"
     CDS             complement(<1..363)
                     /locus_tag="AC162A_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010134.1"
                     /translation="MTLGERLAAYGLVLPPGHTLVSLEERPELRAPLGEFIVPAWAEF
                     LLHDPVANTLWDHLYDDFAAFQCVLFDAQGAIVAGLNSAPLAWDGTDDELPHGWDDQF
                     RRSVEDHLAGTPANTLGAL"
     gene            complement(377..832)
                     /locus_tag="AC162A_04510"
     CDS             complement(377..832)
                     /locus_tag="AC162A_04510"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR5010135.1"
                     /translation="MAPGPGPGVEYAPHGGRLVAYIIDGFIVGIAVTIVALVLTPFLI
                     AGASSENAGALAATGFLYVFVVLLVSVSYFPFFWARNGQTPGMKIFGLRVVRDADGGK
                     ISGGTAIIRLIGLWISFAIFYLGIIWILVDSRRRGWHDLLAGTVVIRQP"
     gene            complement(1025..>2257)
                     /gene="serS"
                     /locus_tag="AC162A_04515"
     CDS             complement(1025..>2257)
                     /gene="serS"
                     /locus_tag="AC162A_04515"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR5010136.1"
                     /translation="AIEVDADRRRLQAESDTRKAERNAASKQIGEAIRGGALPDGPEV
                     AELRAASTSAGERIESIDAELTAAESELEDLLLRIPNPADPSVPVGGEEANVTVRTWG
                     ERASREVAAGDGSTWTRRPHWEIAEALDIIDNVRGAKIAGSGFPVYKGAGSRLQRSLI
                     SWFLDVHTAEHGFTEIWPPVVVNTASARGTGQIPDKEDQMYVVGRDDLYLIPTSEVPV
                     TNLHRDEILEAAELPIRYAAYSPCFRREAGAAGKDTRGILRVHQFDKVEMVLFEKPDD
                     SDAALEWMTARGEVLLQRLGLPYRVLFLSTREMGFVQQKKYDLEVWAPGVERWLEVSS
                     CSNMGDYQARRMAIRYRPTPGAKPELDHTLNGSGLALPRIVAALLEVYQQPDGSVEVP
                     EVLRPYVGATRIGGPPPS"
BASE COUNT          330 a          787 c          822 g          318 t
ORIGIN      
        1 gagagccccg agcgtgttgg cgggagtccc cgccagatgg tcctcgaccg agcgccggaa
       61 ctggtcgtcc cagccgtgcg gcagctcgtc gtcggtgccg tcccacgcga gcggcgcgga
      121 gttgagcccg gcgacgatcg cgccctgcgc gtcgaacagc acgcactgga acgcggcgaa
      181 gtcgtcgtac aggtggtccc agagcgtgtt ggcgaccgga tcgtggagca ggaactcggc
      241 ccacgcgggg acgatgaact cgcccagcgg cgcccgcagc tccggccgtt cctcgagcga
      301 gacgagcgtg tgaccgggcg gcaggaccag cccgtaggcg gcgagtcgct ccccgagcgt
      361 caccgggtcc gcgacgctac ggctgccgga tgactacggt tccggccagc aggtcgtgcc
      421 agccgcgccg gcgactgtcg accaggatcc agatgatccc caggtagaag atcgcgaagc
      481 tgatccacag cccgatcagc cggatgatcg ccgtgccgcc gctgatcttg ccgccgtcgg
      541 cgtcgcggac gacccggagg ccgaagatct tcatgcccgg tgtctggccg ttgcgtgccc
      601 agaagaacgg gaagtagctc accgacacga gcaggacgac gaagacatac aggaacccgg
      661 tcgcggcaag agcgcccgcg ttctccgagg aggcaccggc aatcaggaac ggcgtcagca
      721 cgagggcgac gatcgtgacc gcgatgccga cgatgaagcc gtcgatgatg taggcgacca
      781 ggcggccgcc gtgcggcgcg tactcgacgc ccggaccggg tcctggtgcc acgggtgccg
      841 gcgcccaccc ggccggcgcg cccggcgtgg gtgcgggtgc gggcgctgac gggggcggga
      901 ccgagggcgg cggggctgac gggggcggag aggccggggg ctggctcggg gtggccggcg
      961 gttgcggggc atccatggac atcctcctca tcgccacgac gtccgggcca cgtcacccga
     1021 tggatcagga cggcggcggc ccaccaatcc tagtcgcccc aacatacggt cggaggacct
     1081 cgggcacctc gaccgagccg tcgggctgct ggtacacctc gagcagcgcg gcgacgatcc
     1141 ggggcagggc gaggcccgag ccgttgagcg tgtgatcgag ctcgggcttc gcgccgggcg
     1201 tgggccggta gcggatcgcc atccggcgcg cctggtagtc ccccatgttc gagcagctgc
     1261 tgacctcgag ccagcgctcc acgccgggtg cccacacctc gaggtcgtac ttcttctgct
     1321 gcacgaagcc catctcgcgc gtgctcagga acagcacccg gtagggcagg ccgagccgct
     1381 gcaggaggac ctcgccacgc gccgtcatcc attcgagggc ggcatcgctg tcgtcgggct
     1441 tctcgaacag caccatctcg accttgtcga actggtggac gcgcaggatg ccccgggtgt
     1501 ccttgcccgc ggccccggcc tcccgccgga agcacggcga gtaggcggcg tagcggatcg
     1561 ggagctccgc ggcctcgagg atctcgtcgc gatggaggtt ggtgaccggg acctcggacg
     1621 tcgggatcag gtacaggtcg tcgcgcccga cgacgtacat ctggtcttcc ttgtccggga
     1681 tctggcccgt cccacgcgcc gacgccgtgt tcacgacgac cggcggccag atctcggtga
     1741 acccgtgctc ggcggtgtgc acgtcgagga accacgagat cagcgagcgc tggagccgcg
     1801 agcctgcgcc cttgtagacc gggaacccgg agcccgcgat cttggcgccg cggacgttgt
     1861 cgatgatgtc cagcgcctcg gcgatctccc agtggggccg ccgcgtccag gtgctgccat
     1921 cgcccgcggc gacctcgcgc gacgcccgct cgccccaggt tcggaccgtg acgttggcct
     1981 cttcgccacc gaccggaacg gatggatcgg cggggttcgg gatccgcagc agcaggtcct
     2041 cgagctcgga ctcggcggcg gtcagctcgg catcgatcga ctcgatccgc tcgccggcgc
     2101 tcgtggacgc ggcgcgcagc tcggcgacct cggggccgtc gggcagcgct ccgccgcgga
     2161 tcgcctcgcc gatctgcttc gaggccgcat tgcgctcggc cttgcgcgtg tccgactcgg
     2221 cctggagccg ccgccggtcg gcgtcgacct cgatcgc
//
LOCUS       DBNHXO010000212         3628 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4819682, whole genome shotgun sequence.
ACCESSION   DBNHXO010000212 DBNHXO010000000
VERSION     DBNHXO010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4819682"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /locus_tag="AC162A_04520"
     CDS             complement(<1..1470)
                     /locus_tag="AC162A_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HIR5010137.1"
                     /translation="MRSIRAADVLQLLAIAVPALAIATIAASLLEEVVGVPNASAVYL
                     AAVVVTALVAGTSGAIVAAIAAFLLYDYLFIEPVYTFTVADPGEWLNLVLLLFIGIVV
                     GQLAALQRARTETARAREREARALFQVSRALATRASTPAVLPTIAGILREETAMTRVW
                     IALGTDDAQERVAADTAPDQRPTTPGILLVLQRMPGDTPARWVRVHQPGTRKGSGSGL
                     EACRVRIEGGTRVLGSIWALRDRGRDEPDQTETRLLSAAADQIGQALAHDRLAAESRA
                     AEIARQSDALKSALLQSVSHDLRTPLATIRAAAGTLRPGTGLSADDQLESVEAIDREV
                     EYLNRLVTNLLDLSRIEAGALRAERDVYELDDLVGRTIDRLRARLGDRPLTVDLAAPP
                     VIVDPVFLDEAVTNALENAIKYALDGAQIRVLARPARDEGRVRLTVEDAGPGVPDVTL
                     PLLFDKFYRVPGTPGGSRSGSGIGLAVVRGLVEAMDGRVD"
     gene            1600..2388
                     /locus_tag="AC162A_04525"
     CDS             1600..2388
                     /locus_tag="AC162A_04525"
                     /inference="COORDINATES: protein motif:HMM:NF015669.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HIR5010138.1"
                     /translation="MRRFAFGLAMGAMVLATTACSATGGSTAAAGGTGGTLDGITWRL
                     TSFSSAGTSTNVPAEVAVDALFDSGKVTGTSGCNRYNGPAVVTGATIKIGPLASTQMA
                     CIGPAADVETAYLANLAKAASFTATADALTMYDAAGASILVYAAGPANPLVGEWNVTG
                     YNNGKQAVVSPIAGTTLTATFTEDTVSGSAGCNDYNGGYKLDGDQVTIGPLATTRKAC
                     EQDVMDQEAAFLTALQTPSSVEQAGATVTLRDASGAMQVNLGPK"
     gene            complement(2369..3628)
                     /locus_tag="AC162A_04530"
     CDS             complement(2369..3628)
                     /locus_tag="AC162A_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR5010139.1"
                     /translation="MYRARRLLEPESIEALQELVRSSTRLRVVGSRHAFNDIADTTGD
                     HVSLASLPRVIEIDPEAGTVTVDGAVRYGDLCPVLQGAGLALHNLASLPHISVAGACL
                     TATHGSGDRLGNLSTAVAGLELVRADGELVTIERSTDPDGLAAAVVSLGSLGVVTRLT
                     LDVEPTYRMRQVVFEDLPLAAFRGHLDEITSAADSVSGFTQWRGPVIEQIWTKQRVRD
                     DTSTGPAVSEIGDLFGATAASVPLHPIRGVTPDACTAQLGIAGPWLDRLPHFRLDHTP
                     SAGEELQSEFFVAREHAVAAFDALDALRDRLEPLVFVSEIRTIASDELWLSPAYRRPS
                     VAFHFTWRPDWPSVRALLPAIEAALEPFEPRPHWAKLFTMAPEVVRSRYERLPAFAAM
                     ADRFDPQGTFRNDFTRRYVFGVTSARG"
BASE COUNT          536 a         1255 c         1346 g          491 t
ORIGIN      
        1 gtcgacgcgg ccgtccatcg cctcgacgag ccccctgacg acggcgagcc cgatcccgct
       61 tcccgaccgg gagccgccgg gcgtgcccgg cacccggtag aacttgtcga acagcagggg
      121 cagcgtcacg tccgggacgc cggggccggc gtcctcgacg gtcagcctga cgcgcccctc
      181 atcccgcgcg ggccgcgcga gcacccggat ctgcgctccg tcgagggcgt acttgatcgc
      241 gttctccaat gcgttcgtca ccgcttcgtc gaggaacacg gggtccacga tgaccggtgg
      301 ggccgcgagg tcgacggtca agggccggtc gccgagacgc gcgcgcagcc gatcgatggt
      361 ccggccgacg aggtcgtcga gctcgtacac gtcgcgctcg gcgcgcaagg cgccggcctc
      421 gatccggctc aggtccagga ggttggtcac cagccggttg aggtactcga cctcgcggtc
      481 gatggcctcg acgctctcca gctggtcatc tgcgctcaac ccggtgcccg gccgcagcgt
      541 ccctgcggcc gcgcggatcg tggcgagtgg ggtccgcagg tcgtgtgaca cggactggag
      601 gagggcggac ttgagggcgt cgctctgccg ggcgatctcc gcggcccgcg actcggccgc
      661 taggcggtcg tgggcgaggg cctggccgat ctggtcggcg gcggcggaca gcagcctggt
      721 ctccgtctgg tccggctcgt cgcggccgcg gtcgcgcagc gcccagatcg agccgaggac
      781 acgggttccg ccctcgatcc ggaccctgca tgcctcgagg ccgctgccgc tgcccttccg
      841 ggttcccggc tggtggacgc gcacccaacg ggcgggcgtg tcgccgggca tgcgctggag
      901 cacgagcagg atccccgggg tcgtcggccg ctggtccggc gcggtgtcgg cggccacgcg
      961 ctcctgtgca tcgtccgtcc ccagcgcgat ccagacccga gtcatcgcgg tctcctcgcg
     1021 caggatgcct gcgatcgtgg gcagcacggc tggcgtcgac gcgcgcgtcg cgagcgcgcg
     1081 gctgacctgg aacagcgccc gggcttctcg ctccctcgcg cgcgccgtct cggtcctggc
     1141 tcgctggagt gcggcgagct gacccacgac gatcccgatg aacagcagca ggacgagatt
     1201 gagccactcg cccggatcgg cgacggtgaa cgtgtagacg ggctcgatga acaggtagtc
     1261 gtagaggagg aacgcggcga tggctgccac gatggcgccc gaggtcccgg cgacgagggc
     1321 cgtgacgacg acggcggcga gatacacggc cgacgcgttc gggaccccga cgacttcctc
     1381 gagcagcgag gcagcgatcg tcgcaatggc gagagccggg accgcgatgg cgaggagctg
     1441 cagcacgtcg gctgcgcgga tggagcgcat gccgcgatcg taggcggttc gccgaggctc
     1501 gtcgggacga cggtccgccc atccgcgcta tcgtcgctgc cacgcgcgat cccggggcga
     1561 gtggctctgc atccgatccg cgacacgggg agagtccaca tgcggcgatt tgccttcggc
     1621 ctggcgatgg gtgccatggt cctcgcgacg accgcctgct cggcgaccgg cggctcgacc
     1681 gccgcggcgg gcggaaccgg cggcacgctc gacgggatca cctggaggct gacctcgttc
     1741 tcgtcggcag ggacgtcgac gaacgttccc gccgaggtgg ccgtggacgc gctgttcgac
     1801 agcggcaagg tcacaggcac gagcggctgc aaccgctaca acggtccggc ggtggtcacc
     1861 ggggcgacga tcaagatcgg cccgctcgcc tctacgcaga tggcctgcat cggtccggca
     1921 gccgacgtgg agacggccta cctcgcgaac ctcgccaagg ccgcctcgtt caccgcgact
     1981 gccgacgcgc tgacgatgta cgacgccgcc ggcgcatcga tcctcgtcta cgccgcagga
     2041 cccgccaacc cgctcgtggg cgagtggaac gtcactggct acaacaacgg caagcaggcc
     2101 gtggtctcgc cgatcgccgg caccacgctg accgcgacgt tcaccgagga caccgtgtcg
     2161 ggctcggccg gctgcaacga ctacaacggc ggctacaagc tcgacggtga ccaggtcacc
     2221 atcggcccgc tcgcgacgac gcgcaaggcg tgcgagcagg acgtgatgga ccaggaggcc
     2281 gcgttcctca ccgcgctgca gacgccgtcc agcgtggagc aggccggagc caccgtcacc
     2341 ctccgcgacg cctcgggcgc gatgcaggtc aacctcgggc cgaagtgacc ccgaacacgt
     2401 agcgacgcgt gaagtcgttg cggaacgtgc cctgggggtc gaagcggtcg gccatggcgg
     2461 cgaacgccgg caagcgctca tagcgcgagc ggacgacctc cggcgccatc gtgaacagct
     2521 tggcccagtg cgggcggggc tcgaacggct cgagggcggc ctcgatcgcc ggtagcagcg
     2581 cccggaccga cggccagtcg gggcgccagg tgaaatggaa cgcgactgac ggccggcggt
     2641 aggcggggct gagccagagc tcgtcggacg cgatcgtgcg gatctcgctg acgaacacca
     2701 gcggctcgag gcgatcgcgc agggcatcca gggcgtcgaa cgctgcgacc gcgtgctcgc
     2761 gcgcgacgaa gaactcgctc tggagctcct cgcccgcgct gggcgtgtgg tcgagccgga
     2821 agtgcggcag gcggtctagc cacgggccgg cgatgccgag ctgcgccgtg cacgcgtcgg
     2881 gcgtcacgcc gcgaatgggg tgcagcggga cgctcgcggc ggtcgcgccg aacaggtcgc
     2941 cgatctcgga tacggcgggc ccggtgctcg tgtcatcgcg cacccgttgc ttcgtccaga
     3001 tctgctcgat caccgggccg cgccactgcg tgaaaccgct gacgctgtcc gccgccgagg
     3061 tgatctcgtc gaggtgtccg cggaaggcgg cgagcggcag gtcctcgaac acgacctggc
     3121 gcatccggta ggtcggctcg acgtcgaggg tcaagcgggt cacgacgccg agggaaccca
     3181 gtgagacgac cgccgcggcc agaccgtcgg gatccgtgga tcgctcgatc gtcaccagct
     3241 cgccgtccgc acggacgagc tcgaggccgg cgacggccgt cgacaggttg ccgagccggt
     3301 cgcctgatcc gtgggtcgcg gtgaggcatg cgcccgcgac cgagatgtgc ggcagcgagg
     3361 ccaggttgtg gagggcgagg cccgccccct ggagcaccgg gcacaggtcg ccgtagcgga
     3421 ccgcgccgtc gacggtgacg gtgccggctt cggggtcgat ctcgatgacg cgaggcaggc
     3481 tggcgaggga gacgtggtcg cctgtcgtgt cggcgatgtc gttgaacgcg tgccgcgagc
     3541 cgacgacgcg caggcgcgtc gacgagcgaa ccagctcctg gagcgcctcg atcgactcgg
     3601 gctcgagcag ccgtcgcgcc cgatagat
//
LOCUS       DBNHXO010000213         3162 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1843467, whole genome shotgun sequence.
ACCESSION   DBNHXO010000213 DBNHXO010000000
VERSION     DBNHXO010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1843467"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /gene="cpaB"
                     /locus_tag="AC162A_04535"
     CDS             complement(<1..918)
                     /gene="cpaB"
                     /locus_tag="AC162A_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HIR5010140.1"
                     /translation="MEFEYQDPSRRRGKMIIVAGVIVALVAGGLAFYVLYQARQDAQS
                     SGTVVVPMVVAAVPIAPRVPIAETDIVLRQVPADATNAEGTFADPTALVGLMATVPIL
                     AGQPVYANMLANGTSGTGLAILDPGETVSPESEAWRAVSLTVPDDRAAGGMVQSGDIV
                     DVFVTATITVPEDLAAKGKYTSDKSTKITYQNVKILQRATNYYVVRVSLPVAEEISHL
                     QAAGSGAFSLALRPTEDARTVDVTTLGETTTAMIKRYGLPIPEVYPGSGPITVAPSPT
                     PSPAASPAASPSAGAPSPTPVIEDPESPAP"
     gene            complement(1089..2630)
                     /locus_tag="AC162A_04540"
     CDS             complement(1089..2630)
                     /locus_tag="AC162A_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HIR5010141.1"
                     /translation="MIRRLLAPVRRAGRAGGQILVMFAGGMVLLIGVLAVVIDVGNLW
                     NASIHAQLAAEAAALAGVPYMPGDFSTASSLAVAEAGKNGYSTSTGATVTPALNTESA
                     QRLDVTVSSNFNTFFMGAIGIKTVRVTKTATAEYSLPVPMGSPLSNFGDNSGSFWTVI
                     AAQGSDRGNGDAFAAYYNPNPTLNTQYDAKGYQYAIVVPAGAGSTNIDLYDATFCAVD
                     PTKGTGDRWLSDPTFPSVSTYFTLWSDPAATPLDYADDVAVAQSGTTFEHERQSDRSA
                     TYRDTNYSYPSGTLFALPDCTNTTYHNKWWTLGTVTTPGTYRLQITTTMASNPNDQLG
                     TSASNKFGVRAVTSSSTWKASVYGLGKMAIYANITDGVTNFYLARIDAVHAGKSMVVS
                     LFDPGDASGTASIQVMKPTSTGYTPATFSYTADSNANSCCRSGTNITSLTTVQSGTSR
                     YNNSWVTITIPLPKTYTAPLPPGEPAGTLGGWWKIAYTYNATATDSTTWMVQIRGNPV
                     HLVQP"
     gene            complement(2627..>3162)
                     /locus_tag="AC162A_04545"
     CDS             complement(2627..>3162)
                     /locus_tag="AC162A_04545"
                     /inference="COORDINATES: protein motif:HMM:NF019428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HIR5010142.1"
                     /translation="HDGERGQAVVEFALILPLFVIILFAALEFGSAIDHRTAMAYAVR
                     EGARVGASLGTGGTTPNAVDPAIVAAVQRGLTDPIRMENITSIEIFKANSSGQVTSGY
                     INTYNKDGVLVGTAGWPASTRVTGVSADSIGVRVRYDFHPNTPLSTLIGLFGGGPAPY
                     NVLKMTDTSVMRIEPTP"
BASE COUNT          468 a          984 c         1182 g          528 t
ORIGIN      
        1 cggagccggg ctctcgggat cctcgatgac cggcgtcggc gacggagccc cagccgaggg
       61 cgaggccgcg ggggaggcgg cgggcgacgg ggtcggcgac ggggcaaccg tgatcgggcc
      121 ggagcccggg tagacctcgg ggatcggcag gccgtagcgc ttgatcatcg cggtcgtcgt
      181 ctcgccgagg gtggtgacgt cgaccgtgcg ggcatcctcc gtcgggcgga gggcgaggct
      241 gaaggcgccc gagcccgccg cctggaggtg gctgatctcc tcggccaccg gcagcgagac
      301 gcgcacgacg tagtagttcg tcgcccgctg gaggatcttg acgttctggt acgtgatctt
      361 ggtcgacttg tccgaggtgt acttgccctt ggcggcgagg tcttccggca ccgtgatcgt
      421 ggccgtcacg aagacgtcga cgatgtcgcc ggactggacc atgccgccgg cggcgcggtc
      481 gtccggaacc gtgagcgaga cagctcgcca cgcctcggac tcggggctga ccgtttcacc
      541 cgggtcgagg atggccagcc cggtcccgga cgtgccgttg gccagcatgt tcgcgtagac
      601 gggctgaccg gcaaggatcg ggaccgtcgc catgaggcca accagcgcgg tgggatcagc
      661 gaacgtgccc tcggcgttgg ttgcgtcggc cgggacctgg cgcagcacga tgtccgtctc
      721 ggcgatcggc acgcgaggcg cgatcgggac cgcggcgacg accatcggca ccacgacggt
      781 gcccgaggac tgcgcgtcct ggcgtgcctg gtagaggacg tagaacgcca ggccgcccgc
      841 gacgagcgcc acgatgacgc cggcgacgat gatcatcttg ccgcgacgtc gcgacggatc
      901 ctggtactcg aattccatgc gtgagctacc ttcaggtggg gagtcgattg cgggcgtcgg
      961 gaccgaggac cggagcccct cgtgtcaggc gtctcgggac aatccggtca tggggattgt
     1021 cagcacgcac cacccgcggc acctatggac cgaaagtacc tgcgggcccg gcgcttggga
     1081 tcaggcgatc agggctggac caggtggacg gggttgccgc ggatctggac catccacgtc
     1141 gtcgagtccg tcgcagtggc gttgtaggta taggcgatct tccaccagcc gccgagcgtg
     1201 cccgcgggct cgccaggagg cagcggcgcg gtgtaggtct tgggcagcgg gatcgtgatg
     1261 gtgacccagg agttgttgta gcgactggtc ccgctctgca ccgtcgtcag cgaggtgatg
     1321 ttggtgccgc tgcgacagca cgagttggcg ttgctgtccg ccgtgtagct gaaggtggcg
     1381 ggcgtgtagc cggtcgaggt gggcttcatc acctggatgg acgccgtgcc gctcgcatca
     1441 cccggatcga acagcgacac gaccatgctc ttgccggcgt ggaccgcgtc gatccgggcc
     1501 aggtagaagt tggtcacgcc gtccgtgatg ttggcgtaga tcgccatctt gcccaggccg
     1561 tacacgctcg ccttccacgt ggagctggac gtcacggcgc ggacaccgaa cttgttcgag
     1621 gcgctggtgc ccagctggtc gttcgggttc gaggccatcg tggtggtgat ctggagccgg
     1681 taggtgccgg gcgtcgtcac cgtgcccagc gtccaccact tgttgtggta cgtggtgttg
     1741 gtgcagtccg gcagggcgaa cagcgtcccg ctcgggtagc tgtagttcgt gtcccggtag
     1801 gttgcgctgc ggtcggactg gcgctcgtgc tcgaacgtgg tcccggactg cgccacggcg
     1861 acatcgtcgg cgtagtcgag cggcgttgcg gcggggtccg accacagcgt gaagtaggtg
     1921 gacacggagg ggaacgtcgg gtcgctcagc cagcggtcgc cggtcccctt ggtcgggtcg
     1981 acggcgcaga acgtcgcgtc gtacaggtcg atgttggtcg agcccgcgcc cgcggggacg
     2041 acgatggcgt actggtagcc cttggcgtcg tactgggtat tcagggtggg gttggggttg
     2101 tagtacgccg cgaacgcgtc gccgttgccc cggtccgacc cttgggctgc gatgaccgtc
     2161 cagaagctcc ccgagttgtc gccgaagttg ctgagcgggc tgcccatcgg gaccggcagc
     2221 gagtactccg ccgtcgctgt cttggtcacc cgcacggtct tgatgccgat ggcccccatg
     2281 aagaaggtgt tgaagttcga cgacaccgtc acgtccaggc gctgggcgga ctcggtgttg
     2341 agggccggcg tgacggtcgc gccggtcgat gtcgagtagc cgttcttgcc ggcctcggcg
     2401 acggccagtg acgaggcagt agagaagtcg cccggcatgt acgggacgcc ggcgagcgcg
     2461 gctgcctcag cagccagctg cgcgtgaatg gacgcgttcc acaggttgcc gacgtcgatc
     2521 acgaccgcca ggacgccgat cagcaggacc atcccgcccg cgaacatcac caggatctgg
     2581 ccgccggcgc gacctgcccg gcggacggga gcgagcagcc gccggatcac ggggtcggct
     2641 cgatgcgcat gaccgacgtg tcggtcatct tgaggacgtt gtagggggca gggccaccgc
     2701 cgaacaggcc gatcagcgtc gacagcggcg tgttggggtg gaagtcgtag cgcacccgga
     2761 cgccgatgga gtcggcgctg accccggtca cccgcgtgga ggccggccag cccgcggtcc
     2821 cgaccagcac gccgtccttg ttgtaggtgt tgatgtagcc cgaggttacc tggccggacg
     2881 agttggcctt gaagatctcg atggaggtga tgttctccat ccggatcggg tcggtcaggc
     2941 cgcgctggac cgccgcgacg atcgccgggt cgacggcgtt cggggtcgtg cccccggtac
     3001 ccaacgacgc accgacacgg gccccctcgc gcactgcgta ggccatcgcc gtgcggtggt
     3061 cgatggccga cccgaactcg agcgcggcga acaggatgat cacgaacagc gggaggatca
     3121 gcgcgaactc gacgacagcc tggcctcgct cgccgtcgtg gg
//
LOCUS       DBNHXO010000214         3338 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1644307, whole genome shotgun sequence.
ACCESSION   DBNHXO010000214 DBNHXO010000000
VERSION     DBNHXO010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1644307"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="AC162A_04550"
     CDS             <1..1333
                     /locus_tag="AC162A_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5010143.1"
                     /translation="WTVEDGGRRVTFTLRDDLVFSDGTPLTANDVVHSWRRLFTPGNA
                     SPLASLIADVAGARDLLSGASTDVTTLGVHADGDRTVVVDLERGGGDLPTIVSSAPFA
                     VVPPSVGDGEIAPDPGTLVGSGGYTLERIEEDAWVLAANPRYWAGKPAIDTVRMLTTL
                     AGASPVDAFVEGRADVVPIGFLDAGWIAYDRDLGPSLRSSDSLSVTYYGFDTREPPFD
                     DPLVRQAFAKAVDWRRLAALDEPGSSVAATGMVPAGMPGAPEGDFMPAYDPAGARALL
                     AQAGYASGADLGPISFIANGAGYDQGIVTMLEQNLGVTIDYATMDFATYQARLATDPP
                     RMWSISWVADYPGPNDFLGVLLGTGSTANQGAWSSPEFDAAITDATSAADPAAATAAY
                     AKAMGIVRDQAPVVPVSYGTAFSLVRDGLLGANPNGIGILRLAGLAWADGR"
     gene            1345..2712
                     /locus_tag="AC162A_04555"
     CDS             1345..2712
                     /locus_tag="AC162A_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024876.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HIR5010144.1"
                     /translation="MAVTGLASWVLALAFVTLAQPAFAADVTFGKPTAVANYGTSVTF
                     NVDVTRTVPLDRVELRLLFPEALGPLIVDVPVPPGTGTTTLEYVLDVTGGGHIVPNTP
                     ILSTWAAFTAPGGEPVTSERDRLLYGDTSKDWRTVKGDLVRVHWYAGGEGFAEKALAI
                     GEKAIVDTAALLGVTETDPIDFYIYGDVASFREALGPGTRDNVGGQAHADIRTLFALI
                     TPEDIDQPWVGVVVPHELVHLVFDTAVRNPYRFPPRWLNEGLAVYLSEGYGAQDRGRV
                     EGAVASDELIPLVALGGQFPTDADRTFLAYAESVSAIDYLVREKGQDAVVALVTAYAD
                     GLTDDEAFTRALGLDLAAFQAGWLDDLGASEPQQYGPQPAPPGPLPSGWSGPAPTAGS
                     AATPAAPVDTGVAASRAALSPAPGSTAGSSEAPSYGPVLIVLAILVVGVLAVLVIAQR
                     RGRSA"
     gene            2713..>3338
                     /locus_tag="AC162A_04560"
     CDS             2713..>3338
                     /locus_tag="AC162A_04560"
                     /inference="COORDINATES: protein motif:HMM:NF017734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HIR5010145.1"
                     /translation="MATLAARVRAIPTWQVTLGVALLALGFLIAAQLASEGPRIRITS
                     QERTPLVATALDLQAQQDGLKQQILDTRAAIGALEAAGQGGTTVASDLNGQLQDARIA
                     AGLAALTGPGLVIQLSDSSAPVAQGDDERDYLVSGHDVLAVVEELWLAGAEGVAVNNE
                     RVTVATAIVDIGGSVLANSAYVAPPYQVSAIGPPDMLERLTASPGFVD"
BASE COUNT          439 a         1213 c         1208 g          478 t
ORIGIN      
        1 gtggaccgtg gaggacggcg ggcgccgcgt cacgttcacg ctccgggatg acctggtgtt
       61 cagcgacggg acgccgctca ccgccaacga cgtcgtccac agctggcgcc gcctgttcac
      121 gcccggcaat gcgtcgccgc tggcctcgct catcgccgac gtcgccggcg cccgcgacct
      181 gctcagcggc gcatcgacgg acgtcacgac gctcggcgtg cacgccgatg gcgaccggac
      241 cgtcgtcgtc gacctcgagc gcggcggcgg agacctgccg acgatcgtgt ccagcgcgcc
      301 gttcgcggtc gtgccgccga gcgtgggcga cggcgagatc gcacccgacc ccggcaccct
      361 cgtgggcagc ggcggctaca ccctcgagcg gatcgaggag gacgcctggg tgctggcggc
      421 gaatccgcgc tactgggcgg gcaagcccgc gatcgacacc gttcgcatgc tcacgacgct
      481 ggcgggcgcg agccccgtcg acgccttcgt cgagggccgg gccgacgtcg tgccgatcgg
      541 gttcctcgac gccggctgga tcgcctacga ccgggacctc ggcccgtccc tccggagcag
      601 cgactcgctg tcggtgacgt actacggatt cgacacccgg gagccgccgt tcgacgaccc
      661 gctcgtccgc caggcgttcg cgaaggcggt cgactggcgg cggctggcgg cgctcgatga
      721 gcccgggtcc tcggtcgccg ccaccgggat ggtgccggcc gggatgcccg gcgctccgga
      781 gggcgacttc atgccggcgt acgacccggc gggggccagg gcgctgctgg cgcaggccgg
      841 ctatgcgagt ggcgccgacc tcgggccgat ctcgttcatc gccaacggtg cgggctacga
      901 ccagggcatc gtgacgatgc tcgagcagaa cctgggcgtg acgatcgact acgcgacgat
      961 ggacttcgcc acctaccagg cgaggctggc gacggacccg ccgcggatgt ggagcatctc
     1021 ctgggtcgcc gactacccgg ggcccaacga cttcctgggt gtcctgctgg gcacgggttc
     1081 gacggcgaat caaggcgcct ggtcgtcacc cgagttcgat gccgcgatca cggatgccac
     1141 ctccgccgcc gatcccgccg ccgcgaccgc cgcctacgcg aaggcgatgg ggatcgtgcg
     1201 cgaccaggca ccggtcgtgc cggtgagcta tgggacggcg ttctcactgg tgcgcgatgg
     1261 cctgctgggg gcgaacccga acggaatcgg catcctgcga ctcgccgggc tcgcctgggc
     1321 ggacggccgg tgatccgccg gcgcgtggcg gtcacaggac tggcgtcgtg ggtgctcgct
     1381 ctcgcattcg tcacgctggc gcagccggcc tttgccgcgg acgtcacatt cggcaagccc
     1441 acggccgttg cgaactacgg cacgtctgtc accttcaacg tcgacgtgac gcggaccgtg
     1501 ccgctcgatc gtgtcgagct ccggctgctg ttcccggaag ccctcggccc gctgatcgtc
     1561 gacgtgcccg tgccgccggg gaccgggacc accacgctcg agtacgtgct cgacgtcacc
     1621 ggcggtggcc acatcgtgcc caacaccccg atcctctcga cctgggccgc gttcaccgca
     1681 cccggcggcg agccggtcac gagcgagagg gatcgccttc tctacggcga cacgtcgaag
     1741 gactggcgca ccgtgaaagg cgacctggtg cgggtgcact ggtacgcggg cggcgagggc
     1801 ttcgccgaaa aggcgctggc gatcggcgag aaggcgatcg tcgacaccgc cgcgctgctg
     1861 ggcgtgaccg agaccgaccc gatcgacttc tacatctacg gcgacgtggc gtcgttccgc
     1921 gaggcgctcg gcccggggac gcgggacaac gtcggcgggc aggcgcacgc cgacatccgg
     1981 acgctgttcg ccctgatcac gcccgaggac atcgaccagc cgtgggtcgg cgtcgtcgtt
     2041 ccccacgagc tcgtccacct cgtgttcgac accgccgtcc gcaatcccta ccgcttcccg
     2101 ccgcgctggc tcaacgaggg cctggcggtc tacctctccg agggctacgg ggcccaggac
     2161 cgaggacggg tcgagggggc ggtggcgtcg gacgagctga tcccgctggt cgcgctcggg
     2221 ggccagtttc cgaccgatgc cgaccgcacg ttcctcgcct acgccgagtc cgtctcggcc
     2281 atcgactatc tcgtgcgcga gaaggggcag gacgcggtcg tcgccctggt caccgcctat
     2341 gcggacggcc tcaccgacga cgaggcgttc acccgcgcac tcggcttgga cctcgccgcg
     2401 ttccaggccg gctggctcga cgacctgggt gcgtcggagc cgcagcagta cggtccgcag
     2461 ccggcgccac ccggcccgct gccgtcgggg tggagcggcc ccgcgccaac ggcgggctcg
     2521 gcggcgaccc cggcggcgcc ggtggacacg ggcgtggccg cgtcgcgggc cgcgttgagt
     2581 cccgcgccgg gatcgaccgc gggatcgtcc gaggcgccgt cgtacggtcc ggtgctgatc
     2641 gtcctggcga tcctcgtggt gggcgtcctc gcggtcctcg tcatcgcgca gcggcgcggc
     2701 cggagcgcct gagtggcgac gttggcggcg cgcgtccggg ccatccccac ctggcaggtc
     2761 acgctcggcg tcgcactgct cgccctcggc ttcctgatcg ccgcccagct cgcgtcggag
     2821 ggaccgcgga tccggatcac gagccaggag cggacgccac tggtggccac cgcgctcgac
     2881 ctccaggccc agcaggacgg tctcaaacag cagatcctcg acacccgggc ggccatcggt
     2941 gccctcgagg ccgcggggca gggcggcacg acggtcgcca gcgacctcaa cgggcagctg
     3001 caggacgcgc ggatcgcggc cggcctcgcg gcgctgaccg gacctgggct cgtgatccag
     3061 ctctcggatt cgtccgcgcc ggtcgcccag ggcgacgacg agcgcgacta cctggtctcg
     3121 ggccacgacg tgctggctgt cgtggaggag ctgtggctcg cgggtgccga gggcgtggct
     3181 gtcaacaacg agcgggtgac ggtcgcgacg gcgatcgtcg acatcggcgg ctccgtcctc
     3241 gcgaactccg cctacgtggc gccgccgtac caggtgtcgg cgatcggacc gcccgacatg
     3301 ctcgagcgcc tgacggcctc gccgggcttc gtcgactt
//
LOCUS       DBNHXO010000215         1919 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5018902, whole genome shotgun sequence.
ACCESSION   DBNHXO010000215 DBNHXO010000000
VERSION     DBNHXO010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5018902"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..652)
                     /gene="dcd"
                     /locus_tag="AC162A_04565"
     CDS             complement(53..652)
                     /gene="dcd"
                     /locus_tag="AC162A_04565"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR5010146.1"
                     /translation="MSVLADRDIRAELDAGRVRIDPYDPADLQPSSVDLHLDRTFRVF
                     RNNRYAFIDPRQPQPDLTELLAIEGDEPFILHPGEFVLGQTLEWVELPDDLVARLEGK
                     SSLGRLGLLIHSTAGYVDPGWKGTLTLELSNVANLPIALYAGMRIGQISFFRMSSAVE
                     RPYGSPELGSKYQGQSEPTASAIHADFDRGRRNGAPTEG"
     gene            complement(649..1134)
                     /gene="nrdR"
                     /locus_tag="AC162A_04570"
     CDS             complement(649..1134)
                     /gene="nrdR"
                     /locus_tag="AC162A_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR5010147.1"
                     /translation="MRCPQCGTRDTRVVDSRDLDESTTIRRRRECAACATRFTTYERV
                     EAARLIVAKRDGTRQEFDRDKLADGLAKALTRRPVAADAADRAADAIEAELRSSGLSE
                     IPSSRIGALAMEQLRALDHIAYIRFASVYQSFEDLEDLKREVDSLFAEDTAPKKARRV
                     R"
     gene            complement(1201..1830)
                     /locus_tag="AC162A_04575"
     CDS             complement(1201..1830)
                     /locus_tag="AC162A_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010148.1"
                     /translation="MPEAALLWLGLLVAVLLGLFLVTARRMSKLIARTRDLEAFQRAI
                     ADLDASLGTVVDPLVTQLDEIRRRAGDPVSLSRGLDAIQGHLRELVGRGRELRPPRAL
                     ADDATAMLAELDRASRATDLLEHGLDALLAHRGHRELEAQTSLKRAALNLRNARGAAA
                     SIARRVAAVRPADLLTMPDGKETVRVKAPSSIGFDGLDGDDDLVQHPRM"
BASE COUNT          264 a          676 c          705 g          274 t
ORIGIN      
        1 tccccccgcc gaggtccgcg ccccagtcgc cgccgtcctc gaggcccgtg gcctagccct
       61 ccgtgggcgc gccgttcctg cggccgcgat cgaagtcggc gtggatggcg gacgccgtcg
      121 gttccgactg gccctggtac ttcgatccga gctcggggct gccgtacggc cgctcgaccg
      181 ccgagctcat ccggaagaag ctgatctgcc cgatgcgcat gccggcgtag agggcgatcg
      241 gcaggttggc gacgttggac agctcgagcg tgagcgtgcc cttccagccc ggatcgacgt
      301 agcccgcggt cgagtggatg agcagcccaa ggcggccaag actcgacttc ccttcgagcc
      361 gcgcgaccag gtcgtcgggc agctcgaccc actcgagggt ctggcccagc acgaactcgc
      421 ccgggtgcag gatgaacggc tcgtcgccct cgatcgcgag cagctccgtg aggtcgggct
      481 gcggctgacg tgggtcgatg aacgcgtacc ggttgttgcg gaagacgcgg aacgtgcggt
      541 cgaggtggag gtccaccgac gacggctgga ggtcggccgg atcgtaggga tcgatgcgga
      601 cgcgccccgc gtcgagctcg gcccggatgt cccggtcggc gaggacgctc atcgcacccg
      661 tcgcgccttc ttgggtgcgg tgtcctccgc gaacagcgag tccacctcgc gcttgaggtc
      721 ctcgaggtcc tcgaagctct ggtagacgga cgcaaagcgg atgtaggcga tgtggtcgag
      781 ggcgcggagc tgttccatcg cgagggcgcc gatccgcgag ctcggaatct ccgacaggcc
      841 cgacgaccgc agctcggcct cgatggcgtc ggcggcgcga tcggcggcat cggcggccac
      901 ggggcgtcgt gtcagcgcct tggcgaggcc gtcggccagc ttgtcgcggt cgaactcctg
      961 gcgcgtcccg tcgcgcttgg cgacgatcag cctcgcggcc tcgacgcgct cgtaggtcgt
     1021 gaaccgcgtc gcgcatgccg cgcactcgcg gcggcggcgg atcgtggtcg actcgtcgag
     1081 gtcgcgggag tctacgacac gcgtgtcacg ggtgccgcat tgcgggcagc gcatcgaccc
     1141 tccaggtcct ccgaatgtcc cccacaccac aagccctagt ggtgtgtcga gaggatacca
     1201 ctacatgcgg gggtgctgca ccaggtcgtc gtcaccgtcg aggccgtcga agccgattga
     1261 cgacggcgcc ttgacccgca cggtctcctt gccgtcgggc atggtcagca ggtcggccgg
     1321 ccgcaccgcg gcgacacgtc gggcgatgct cgccgccgcg ccgcgcgcgt tgcgcaggtt
     1381 gagcgccgcc cgcttgagcg aggtctgggc ctcgagctcc cggtgcccgc gatgggcgag
     1441 cagcgcatcg aggccgtgct cgagcaggtc cgtggcccgc gacgcccggt cgagctcggc
     1501 cagcatcgcc gtggcgtcgt cggccaacgc gcgcggcggc cgcagctccc tgccgcgccc
     1561 gacgagctcg cgcaggtgcc cctggatcgc atccagcccg cgggacagcg ataccgggtc
     1621 gcccgcacgc cgccggatct cgtcgagctg ggtgacgagg gggtcgacga cggtcccgag
     1681 cgacgcgtcg aggtcggcga tcgcgcgctg gaacgcctcg aggtcacgcg tccgcgcgat
     1741 gagcttggac atccgacgcg cggtgaccag gaacagcccg agcaggacgg caacgagcag
     1801 acccagccag aggagggcag cctcaggcac cgcgccctgc tccgtgcccg ctcgccggcc
     1861 ggcgcgtcag ctgccgcgcc gcgagcgcag ctcggcgacg agggccgggc cgacctcga
//
LOCUS       DBNHXO010000216         3477 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2241633, whole genome shotgun sequence.
ACCESSION   DBNHXO010000216 DBNHXO010000000
VERSION     DBNHXO010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2241633"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="AC162A_04580"
     CDS             complement(<1..757)
                     /locus_tag="AC162A_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR5010149.1"
                     /translation="MALIGRADELAAIVRALDGDAPIVVVGEAGIGKTTLVRAAVAAT
                     ARPIHEGGAFATLAWMPYLVLERALGGPLSGDADWVSDAVERRVGPDILFVDDAQWAD
                     ERSLAAIERLAGRISIVVATRRGDEGTAAVLAALGPAGPVILELAPLDADDAALVALA
                     TEPGLTRERAAVIGARAGGNPFVIEQLAATGEPTSGLRRAIAARLDGLEPSDRQLLGM
                     LALAGRPLPTGSVDGHERLVRNALAVLTPDGGVA"
     gene            complement(786..1340)
                     /locus_tag="AC162A_04585"
     CDS             complement(786..1340)
                     /locus_tag="AC162A_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5010150.1"
                     /translation="MPAPVARAARQPLIVIEGPPGTAERAASSLRAAGFDVVAGFRVP
                     IVSDRAPSTPPIVCSGAVADGAGASEALLAAIAGAGILVDAIADRVTVDRLVDDLRRV
                     GPVDHRIVTTQTTDPVVSPESRAILGLLAEGHSLGEAAAALGLSRRTADRRLHEARDA
                     LGVARTTEAIARAASLGLLGTTNE"
     gene            complement(1672..1887)
                     /locus_tag="AC162A_04590"
     CDS             complement(1672..1887)
                     /locus_tag="AC162A_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010151.1"
                     /translation="MRHTATSYGVSLNLAHDRAHRDHQAADAWRRLHTEPDAALDAAV
                     DGGHRSFRPIVARLVASLSVRRARSTT"
     gene            2041..3258
                     /locus_tag="AC162A_04595"
     CDS             2041..3258
                     /locus_tag="AC162A_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019545401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR5010152.1"
                     /translation="MHDAFATRVVHAGAEPDETTGAVSPPIYQTSTYAQDGVGNPRGG
                     YEYARSQNPTRERLERAVADLEGGRHGIAFASGSATTAAIAQLAAEGDEIVVGDDVYG
                     GTYRYLERVHRPAGAGEARYVDLAAGPDALWEALTEQTRLVWFETPSNPMLKITDVAA
                     SAALVRDRAAQAGRERPLIVVDNTFASPALQRPLELGADIVFHSATKFLGGHSDAILG
                     IAVTADDAVAERLRFLQNAMGGVPGPFDCFLVLRGIRTLALRMERHCANAMAVARFLA
                     ARDDVAWVGYPGLAEGRHAHPGHETAARQMRLGGEPAFGGMVSFMPADGGRHERSGPE
                     RARTVCEATRLFTLAESLGSVESLIEVPAAMTHLSVTESALAVDPALVRLSVGIEAAE
                     DLIADLSRALDDA"
     gene            complement(3276..>3477)
                     /locus_tag="AC162A_04600"
     CDS             complement(3276..>3477)
                     /locus_tag="AC162A_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010153.1"
                     /translation="GPVTIQQHTRVSGTAQLTLNDTDGTALMKFTERSRRQWAEVPRG
                     YGAGPDTAQPMTDLTWSPDAGC"
BASE COUNT          437 a         1282 c         1309 g          449 t
ORIGIN      
        1 tggccacccc gccgtcgggc gtgagcaccg cgagggcatt ccgtacgagg cgctcgtggc
       61 cgtccacgga gcccgtgggt agcggccgcc cggcgagggc gagcatgccc agcagctgtc
      121 gatccgaggg ttcgagcccg tcgagccgcg cggcgatcgc gcgccggagg cccgacgtcg
      181 gctcgccggt ggcggccagc tgctcgatga cgaatgggtt tccgcccgcc cgcgcgccga
      241 tcaccgcggc gcgctcgcgg gtcagccccg gctcagtggc gagggccacc agggccgcgt
      301 cgtcggcgtc gagcggcgcg agctccagga tcaccggacc ggcaggcccg agggctgcga
      361 gcacggccgc cgtcccctcg tcgccccggc gggtcgcgac cacgattgag atccgcccgg
      421 cgagccgctc gatcgcggcc agggatcgtt cgtccgccca ctgggcgtcg tcgacgaaca
      481 ggatgtcggg tccgacgcgc cgttcgacgg catccgagac ccagtcggcg tcaccggaca
      541 gcggacctcc caaagcgcgc tccaggacga ggtagggcat ccaggcgagc gtcgcgaacg
      601 ccccgccctc gtggatcggg cgtgcggtcg ccgccaccgc cgcgcggacg agcgtcgtct
      661 tgccgatccc ggcctcgccg accaccacga ttggggcgtc gccatccagc gcgcggacga
      721 tggcggccag ctcgtccgcg cgcccgatca gcgccatcga tcgacccctc gcgcggcgcg
      781 gaccctcact cgttcgtcgt cccgagcagg ccgagcgatg cggctcgtgc gatcgcctcg
      841 gtggtccgcg cgacgcccag tgcgtcgcgt gcctcgtgga gccggcgatc ggcggttcgg
      901 cgggacaggc cgagggccgc cgcggcctcg cccagcgagt ggccctcggc cagcaggccg
      961 aggatggccc gggactcggg cgagaccacc gggtcggtgg tctgggtggt gacgatccga
     1021 tggtcgacgg gcccgacacg gcggaggtcg tcgacgaggc gatcgacggt gacccggtcc
     1081 gcgatggcgt cgacgaggat gccggccccg gcgatcgccg cgaggagggc ctcgctcgcg
     1141 cccgccccgt cggccacggc gcccgaacac acgatgggcg gcgtcgaagg cgcccggtcg
     1201 ctcacgatcg gaaccctgaa gcccgcgacg acatcgaagc ccgcggcccg caggctggac
     1261 gcggcccgct cggcggtgcc gggcggcccc tcgatcacga tcagcggttg gcgcgccgca
     1321 cgggcgactg gagcgggcat ggcgtctctg tctgggcgga ccaggcgggg cgccggcggc
     1381 gcggcgtagg cggcatggtg gcagccgtgc gccgaggaat caaaccgcga accgggcgac
     1441 gggaccctcg atggcgcagc tggacgatga cgttgccttg cccacaccgc acggtggccg
     1501 catggttggg atccccgccc gtccgcggcg tccgccgcga ccggccccgc cgacggccgg
     1561 tcctgcgcgt gcaccgcgaa ggcgccggac gaccgtcggc cgtccgctcg tgccgtcgcg
     1621 cgccaacccg gcgacgcgcg atccgcgacg ggcgcgtccg ccgccccgca cctaggtcgt
     1681 ggagcgcgcc cgccgaacgc tgaggctggc gacgaggcgc gcgacgatcg gccggaagct
     1741 gcggtggccg ccatcgaccg ccgcgtcgag cgccgcgtcg ggctctgtgt gcaggcgacg
     1801 ccaggcgtcg gctgcctggt gatcgcgatg ggcgcggtcg tgggcgaggt tgaggctcac
     1861 gccgtaggag gttgcggtgt ggcgcatcgg tcgggtctcc ggtgtggcgg ctgactggcg
     1921 gggcatgacc acaccttggc cgtaaggccc gccgtgggcc atccggcgat gccttattcc
     1981 cgccccgttc cggcctcaat ctggcggccg acgacgcgcc gacgatgcga tgatcggcgc
     2041 atgcacgacg ccttcgccac gcgagtcgtt cacgcgggtg ccgagccgga cgagacgacc
     2101 ggcgcggtgt ccccgccgat ctaccagacg tcgacgtacg cccaggacgg cgtgggcaac
     2161 cctcgcggcg ggtacgagta cgcgcgcagc cagaacccca cccgcgagcg gctcgagcga
     2221 gccgtcgccg acctcgaggg cggccggcac gggatcgcct tcgccagcgg ctccgcgacc
     2281 accgcggcca tcgcgcagct ggcggcggag ggcgacgaga tcgtcgtcgg cgacgacgtc
     2341 tacggcggca cgtaccgcta cctggagcgc gtccatcgtc ccgccggggc cggggaggcc
     2401 cgctacgtcg acctcgccgc cggacccgat gcgttgtggg aggcgctgac ggagcagacg
     2461 cgactcgtct ggttcgagac gccgtccaac ccgatgctca agatcaccga cgtcgccgcg
     2521 tcggccgcgc tcgtccgcga tcgcgcggca caggcggggc gcgagcggcc gctgatcgtc
     2581 gtcgacaaca ccttcgcgtc cccggccctc cagcgaccgc tcgagctggg cgcggacatc
     2641 gtgttccact cggcgaccaa gttcctgggc ggccactcgg acgcgatcct gggcatcgcc
     2701 gtcaccgcgg acgatgcggt cgccgagcgc ctgcggttcc tgcagaacgc catgggcggc
     2761 gtccccgggc cgttcgactg cttcctcgtc ctgcggggca tccggacgct cgctctgcgg
     2821 atggagcgcc actgcgccaa cgccatggcc gtcgccaggt tccttgccgc gcgcgacgac
     2881 gtcgcctggg tcggctaccc ggggctcgcg gagggccgcc acgcgcaccc gggccacgag
     2941 accgccgcgc gccagatgcg gctcggcggg gagccggcgt tcgggggcat ggtctcgttc
     3001 atgccggccg acggtgggcg gcatgaacgc agcggtcccg agcgggcccg aacggtctgc
     3061 gaggccaccc ggctgttcac gctcgctgag tcgctgggca gcgtcgagtc attgatcgag
     3121 gtcccggccg cgatgacgca cctgtcggtg acggagtcgg cgctggccgt ggacccggcc
     3181 ttggtccggc tgtcggtcgg gatcgaagcc gccgaggacc tgatcgcgga cctgtcgcgg
     3241 gcgctcgacg acgcctgacg gagccccgac gcccctcagc agccggcgtc tggcgaccag
     3301 gtcaggtcgg tcatcggctg ggccgtatcg gggccggctc cgtacccgcg gggaacctct
     3361 gcccactgcc gccgcgaccg ctccgtgaat ttcatgagcg cggtcccgtc ggtgtcgttg
     3421 agcgtgagct gtgccgtgcc gctgacgcgc gtgtgctgct ggatcgtgac gggcccg
//
LOCUS       DBNHXO010000217         2653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3629643, whole genome shotgun sequence.
ACCESSION   DBNHXO010000217 DBNHXO010000000
VERSION     DBNHXO010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3629643"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="AC162A_04605"
     CDS             complement(<1..410)
                     /locus_tag="AC162A_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HIR5010154.1"
                     /translation="MSDDPTTFLDVVEPPEATAAAPGQAATSRAGGATLALARAGLIV
                     TVAFLLSRILGYVRYVAIAAAVPDAGQLDAFFAAFRIPDFLFQLVAAGALSSALIPII
                     AGLLANDEEARAWRVVSSVTTLMLGTLAILAAVVL"
     gene            complement(407..1378)
                     /locus_tag="AC162A_04610"
     CDS             complement(407..1378)
                     /locus_tag="AC162A_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR5010155.1"
                     /translation="MADDVTGDEALRRFVRSLEARDTSPHTRRSYEATVVAYLDWLAA
                     QDADWRSPGRAVLRGYLAELGTGRARSSVAQRLAALRAFHRYAARSGLAPGDPWGAIA
                     TPRQPRRLPRVLEVDQVERLIAVVEADLDAADAATGRGHDPDGTRAIALRDRALVETA
                     YAAGLRISELAAAELGNLDLRRGELRVLGKGRKERIGLLGRPARDALEAYLAEGRPVL
                     AARRSPLDEPTTAVFLNHLGAPLGVRGLRGRLDRLRRLAGLPEGVSPHTLRHSFATHL
                     LEGGADLRVVQELLGHESLATTQVYTHVSPARLQEAYRLAHPRARNS"
     gene            complement(1436..>2653)
                     /locus_tag="AC162A_04615"
     CDS             complement(1436..>2653)
                     /locus_tag="AC162A_04615"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HIR5010156.1"
                     /translation="GDYRLRASATRTLFDGFARVYTEGQDDAAEEAERTLPPLAKGDV
                     TRVLDVTPSQHFTEPPPRFTEATLIKALEEHGIGRPSTYAATISTIVDRGYVRIEERR
                     LHPEEIGEIVTDFLVLYFSEYVDLAFTARMEEELDEVAEGGRAWVPLLRAFFGPLKER
                     VDEVRKTVKRSDITTEASDEVCSEGHPMVIRLGRNGKFLACSTYPEHKETRPLPGEEA
                     PKLEGDGDPCPQCGEGILTTRRGKFGAFVGCSRYPDCTYIRRDGPPPPDQLPFEVMCP
                     KNGDGKLVARRARRTGNVFWGCSNYPRCDFTTNFEPTGAVHDAHEDGRGAIARRPDGG
                     LCLTCGATVELPEGDLVGRKLAGGPADPAALERPARGGGRRGGSRSGGGARRGGRGAG
                     TSRSGAGTRRSGE"
BASE COUNT          345 a          985 c          984 g          339 t
ORIGIN      
        1 aggaccacgg ccgccaggat cgcgagggtc ccgagcatca acgtcgtcac gctcgacacg
       61 acgcgccacg cccgcgcctc ctcgtcgttc gccagcagcc cggcgatgat cgggatcaac
      121 gcggacgaga gcgcgccggc ggcaaccagc tgaaacagga agtcggggat ccggaacgcg
      181 gcgaagaacg cgtcgagctg cccggcgtcg ggcaccgcgg cggcgatcgc cacgtagcgg
      241 acgtagccga ggatccgaga gagcaggaac gcgaccgtga cgatgagccc ggcgcgggcc
      301 agggcgaggg tggccccgcc tgcccggctc gtcgccgcct ggcccggggc ggccgccgtc
      361 gcctccggtg gctccacgac gtcgaggaac gtcgtcgggt cgtcgctcac gagttgcgag
      421 ccctcgggtg ggcgagccgg tacgcctcct gcagccgcgc gggcgacacg tgggtgtaca
      481 cctgcgtcgt cgcgaggctt tcgtggccca gcagctcctg cacgacgcgc aggtccgcgc
      541 cgccctcgag caggtgcgtt gcgaagctgt ggcgcagcgt gtgcgggctg acgccctccg
      601 gcagcccggc gaggcgccgg agacggtcga ggcgcccacg caggccgcgg acacccagcg
      661 gcgcgccaag gtggttgagg aagaccgccg tcgtcggttc gtccagaggc gatcgccggg
      721 cggccaggac gggccgtccc tccgcgaggt aggcctcgag cgcgtcccgg gccgggcggc
      781 cgaggagccc gatgcgctcc ttgcgcccct tgccgagcac ccggagctcc ccgcgccgca
      841 ggtccaggtt gccgagctcg gccgcagcca gctcactgat ccgcagcccg gctgcgtatg
      901 ccgtctcgac gagcgcccgg tcacgcagcg cgatggcccg cgtgccgtcc gggtcgtgac
      961 cgcggcccgt ggcggcgtcc gcggcgtcga ggtccgcctc gaccacggcg atcagccgct
     1021 cgacctggtc gacctcgagc acccgcggga ggcggcgcgg ctgccggggc gtggcgatcg
     1081 cgccccatgg atcgccgggc gcgagccccg acctcgcggc gtagcggtgg aacgcccgca
     1141 atgcagcgag gcgctgggcg acggacgacc gtgcgcgccc ggtaccgagc tcggcgagat
     1201 agccgcgcag gaccgcccgc ccgggggacc gccagtcggc atcctgggcc gccagccagt
     1261 ccaggtacgc gacgaccgtc gcctcgtacg agcgccgcgt gtgcggcgac gtgtcgcgcg
     1321 cctcgagcga gcggacgaag cggcgcagcg cctcgtcgcc ggtcacgtcg tcagccattt
     1381 gcccattgtg gggagcaacc gccccccgtg acgcgccgcg gcgtggggcc ggggatcact
     1441 cgcccgagcg ccgcgtcccg gctccggacc gcgaggtgcc tgcgccccgg cccccgcggc
     1501 gggctccccc gccgctccgt gagccgccac gccggccacc gccgcgcgcc ggtcgctcca
     1561 gcgcggcggg gtcggccggg ccgccggcca gcttccgtcc gaccaggtcg ccctcgggca
     1621 gctcgaccgt cgcgccacag gtcaggcaga ggccgccgtc cgggcgtcgc gcgatcgccc
     1681 cacgaccatc ctcgtgggca tcgtgaacgg cgcccgtcgg ctcgaagttt gtcgtgaagt
     1741 cgcagcgcgg gtagttggag cacccccaga agacgttgcc ggtccgccgc gcgcgacgcg
     1801 ccacgagctt gccgtcgccg ttcttggggc acatcacctc gaacgggagc tgatcgggcg
     1861 gcggcgggcc gtcccggcgg atgtacgtgc agtccggata gcgcgagcag ccgacgaacg
     1921 caccgaactt gccgcgccgg gtcgtgagga tgccctcgcc gcactgcggg cagggatcgc
     1981 cgtccccctc gagcttgggc gcctcctccc ccggcagggg gcgcgtctcc ttgtgctcgg
     2041 ggtaggtcga gcaggcgagg aacttcccgt tgcggccgag gcggatgacc atcgggtggc
     2101 cctccgagca gacctcgtcg ctggcctcgg tggtgatgtc gctgcgcttg acggtcttgc
     2161 ggacctcgtc gacgcgctcc ttgagcgggc cgaagaacgc ccgcaggagc gggacccacg
     2221 cccggccgcc ctcggcgacc tcgtcgagct cctcctccat ccgggccgtg aacgcgaggt
     2281 ccacgtactc gctgaagtac aggaccagga agtcggtcac gatctcgccg atctcctcgg
     2341 gatgcaggcg ccgctcctcg atccggacat agcctcgatc gacgatcgtc gagatcgtcg
     2401 cggcatacgt cgacgggcgg ccgatgccgt gctcctcgag cgccttgatc aacgtcgctt
     2461 ccgtgaaccg cggcgggggc tcggtgaagt gctggctggg cgtgacgtcg agcacgcggg
     2521 tgacgtcgcc cttggccagc ggcggcagcg tgcgctcggc ctcctcggcg gcgtcgtcct
     2581 ggccctccgt gtagacccgg gcgaagccgt cgaacagggt ccgggtcgcc gaggcgcgaa
     2641 gccggtagtc gcc
//
LOCUS       DBNHXO010000218         3410 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2042424, whole genome shotgun sequence.
ACCESSION   DBNHXO010000218 DBNHXO010000000
VERSION     DBNHXO010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2042424"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="AC162A_04620"
     CDS             complement(<1..682)
                     /locus_tag="AC162A_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019932712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HIR5010157.1"
                     /translation="MITATPPFRRVLVANRGEIAVRIIRACRDQGMESVAVFSDADAG
                     AAHVRLADVAVRLGPPPPAESYLRIDAIVEAAVASGAEAVHPGYGFLAERAAFARAVE
                     DAGLVYVGPSPASIEALGDKLNARRLATSVNVPVVPGTLEPAPVERSNEVAGIVAAAE
                     RIGFPLLVKAAAGGGGRGMRRVERADELPAALASGSHEAASAFGDGSVYLEREILPAR
                     HVEVQLLAD"
     gene            complement(737..2269)
                     /locus_tag="AC162A_04625"
     CDS             complement(737..2269)
                     /locus_tag="AC162A_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR5010158.1"
                     /translation="MARLEQLRAESREGGGAERAERQHAAGKLTARERLDLLLDADSF
                     VELDAFVTHRATEFGLDEQRVLGDGVVTGHGTIDGRLVFVYSQDFTVFGGSLSEAYAE
                     KICKVMDLAMKVGAPIVGLNDSGGARIQEGVASLGGYAEIFLRNVLASGVVPQISVIL
                     GPCAGGAVYSPAMTDFTVMVEGTSYMFVTGPNVVRAVTHEVVDAEELGGAAVHTGRSG
                     VAHLAALDEADAMDAVRRLLAHLPQNNLADAPTIASGDPRDRMDTDLDSLVPDDPSRP
                     YDMHDVIARVVDDGAFLEIQSAWAANILGGFARLGGRAVGVVAQQPAVLAGALDIDAS
                     VKAARFVRTCDAFNVPLVTLVDVPGFLPGVAQEHGGIIRHGAKLLYAYCEATVPKVTV
                     ITRKAYGGAYDVMSSKHIRGDFNFAWPTAEIAVMGAEGAVNIIFKDEIAGAVDSDAER
                     VRLVAAYEAEFSNPYVAAARGYIDEVILPSETRPRLIRALEVLANKQDTNPRKKHGNI
                     PL"
     gene            2633..>3410
                     /locus_tag="AC162A_04630"
     CDS             2633..>3410
                     /locus_tag="AC162A_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR5010159.1"
                     /translation="MTPLTHVIAIANQKGGVGKTTTAINLAGALAEEGFRVLLVDMDP
                     QANLTAGVGINLNTITWSISDVLSDGRATMEQVILATETAGIDVVPAHIDLASTEGEL
                     FTALGRESILREAMRDAIVNYDFVLIDCPPNLGLLTVNGLVAADSVIIPVQTQFYAMK
                     GLNNLVKVINAIRLKLNRDLKILGLLPTFYDARTNLARDMLDELRVVGDQHVFSSIIR
                     NTVKLGEAPLVGRPITSYAGTSEAARTYREFAREVIDLGQD"
BASE COUNT          539 a         1208 c         1149 g          514 t
ORIGIN      
        1 cgtccgccag gagctggacc tcgacatgac gcgccggaag gatctcacgc tcgaggtaga
       61 cggatccgtc gccaaaggcc gatgccgcct cgtgggatcc ggacgccagt gcggccggca
      121 gctcgtcagc gcgctcgacg cggcgcatgc cgcggcctcc cccaccggcg gccgccttga
      181 cgagcagcgg aaagccgatc cgctcggcgg ccgcgacgat accggcgacc tcgttggaac
      241 gctcgaccgg cgcaggctcg agcgttcccg ggaccaccgg gacgttcacc gacgtcgcga
      301 ggcgtcgcgc gttgagcttg tcgccgagcg cctcgatgct tgccggcgac ggtccgacgt
      361 agacgagtcc cgcatcctcg acggcgcggg caaacgcggc ccgctccgcg aggaacccgt
      421 agcccgggtg gaccgcctcg gcccccgacg cgacggcggc ctcgacgatc gcgtcgatac
      481 gcagataact ctcggccggt ggcggcggtc cgagcctgac ggcgacgtcc gccagacgca
      541 catgtgccgc accggcgtcg gcgtcgctga agaccgcgac ggattccatc ccctggtcgc
      601 gacaggcgcg gatgatgcgc accgcaatct cgccccggtt ggcgaccagg acgcgccgaa
      661 agggcggggt ggcggtgatc atcgcgggcg gccgccgcgc tcgtctttgc acaaagacgg
      721 cgaccggccg cgcgcgtcag agcgggatgt tgccatgctt cttgcggggg ttcgtgtcct
      781 gcttgttcgc gagcacctcg agtgcgcgga tgaggcgcgg tcgggtctcg ctcggcagga
      841 tcacctcgtc gatgtagccg cgcgcggcag cgacgtaggg attcgagaac tcggcctcgt
      901 aggcggcaac cagccggacc cgctccgcgt cggagtcgac cgcgcctgcg atctcgtcct
      961 tgaagatgat gttgaccgcg ccctcggcac ccatgacagc gatctcggcc gtcggccacg
     1021 cgaagttgaa gtcgccgcgg atgtgcttgc tgctcatgac gtcgtacgcg ccgccgtacg
     1081 ccttgcgcgt gatgaccgtc accttcggca cggtcgcctc gcagtacgcg tagagcagct
     1141 tcgcgccgtg gcggatgatc ccgccgtgct cctgcgcaac gcccggcagg aagcccggga
     1201 cgtcgacgag cgtgaccagc ggcacgttga acgcgtcaca ggtgcgcacg aatcgcgctg
     1261 ccttcacgga ggcgtcgatg tcgagcgcgc ccgccagcac ggcgggctgc tgggcgacga
     1321 cgccgaccgc gcgcccgccg agtcgcgcga agccgcccag gatgttcgcg gcccaggccg
     1381 actggatctc gaggaacgca ccgtcatcga cgacgcgcgc gatgacgtcg tgcatgtcgt
     1441 atggccgcga ggggtcgtcg ggtacgagcg aatcgagatc ggtgtccatg cgatcgcggg
     1501 gatcgccgct cgcgatcgtc ggcgcatccg cgaggttgtt ctgcggcagg tgcgccagca
     1561 gccggcgcac cgcgtccatc gcgtccgcct cgtcgagggc ggccagatgc gcgacgccac
     1621 tccggcccgt gtggaccgcg gcgccgccaa gctcctcggc gtcaaccacc tcgtgggtca
     1681 cggcgcgcac gacgttgggg ccggtgacga acatgtagct cgtcccctcg accatcaccg
     1741 tgaagtcggt catcgccggc gagtagacag cgccgcccgc gcacggcccg aggatcacgg
     1801 agatctgcgg cacgacacca gaggccagga cgttgcgcag gaagatctcg gcgtagcccc
     1861 cgagtgacgc cacgccctcc tggatccgcg cgccgcccga atcgttgagc ccgacaatcg
     1921 gcgcgccaac cttcatcgcc agatccatca ccttgcagat cttctcggcg taggcctcgg
     1981 acagcgatcc gccgaagacc gtgaagtcct ggctgtagac aaagacgagg cgaccgtcga
     2041 tggtgccgtg gcccgtgacg acgccatcgc ccaggacgcg ctgctcgtcg agcccgaact
     2101 cggtcgctcg atgggtcacg aatgcgtcga gctcgacgaa cgagtcggcg tcgagcagca
     2161 ggtcgaggcg ttcgcgagcc gtcagcttgc ccgcggcgtg ctggcgctcg gcccgctccg
     2221 ctcccccgcc ttccctggac tcggcccgaa gctgctcgag tcgcgccatc cgctccgcgt
     2281 tggtcgacac gtcgggggct ccctccggtg cgctgcgcgc tgtccttgcg caaagacgtg
     2341 gccgcttgga cccatcatag gcggcccgcg tcgccggccg ggaccgttcg ggcggcggga
     2401 tgcgccgggt cgccaaacgg ccacaggcgg tgttgtcggg gcatcaacgg ctcgttagcg
     2461 gttgccgtgg cgcaccgctg aatcaccgcg acgggcttgc ggatatccac agaacggtgc
     2521 acaactcggt cattgtgtgg acaacctcga cgggcgctcc gtcgagcccg ggggtaggat
     2581 tcgaccactt tcgccgacct gccggcatcc gtcacgcccc tggatccccg aggtgacgcc
     2641 cttgacgcac gtgatcgcga tcgccaacca gaagggcggc gtcggcaaga cgacgaccgc
     2701 aatcaacctc gcgggggcgc tcgctgagga agggtttcgc gtcctgctgg tcgacatgga
     2761 cccacaggcg aacctgaccg caggcgtcgg tatcaacctc aacacgatca cgtggtcgat
     2821 ctccgacgta ctctctgacg ggcgcgcgac gatggagcag gtgatcctcg cgaccgagac
     2881 cgcgggcatc gacgtcgtcc cggcgcatat cgacctcgcc agcacggagg gcgagctgtt
     2941 cacggcgctg ggccgcgagt cgatcctgcg cgaggcgatg cgcgacgcga tcgtgaacta
     3001 cgacttcgtc ctgatcgact gcccgcccaa cctcgggctg ctgacggtga acggcctcgt
     3061 cgcggcggac agcgtgatca tccccgtcca gacccagttc tacgcgatga agggcctcaa
     3121 caacctcgtc aaggtcatca acgccatccg cctcaagctc aaccgggatc tcaagatcct
     3181 cggcctcctc cccacgttct acgacgcccg caccaacctc gcgcgcgaca tgctggacga
     3241 gctgcgcgtg gtcggcgacc agcacgtctt ctcctcgatc attcgcaaca ccgtgaagct
     3301 gggggaggcg ccgctcgtcg ggcggccgat cacgtcctat gcggggacct ccgaggccgc
     3361 ccgaacctac cgcgagtttg cccgggaggt catcgatctt ggccaggact
//
LOCUS       DBNHXO010000219         2056 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4622668, whole genome shotgun sequence.
ACCESSION   DBNHXO010000219 DBNHXO010000000
VERSION     DBNHXO010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4622668"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="AC162A_04635"
     CDS             <1..1291
                     /locus_tag="AC162A_04635"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR5010160.1"
                     /translation="DAPWRLPSVNLVMDDDQPMSHVFEAAAAAVGLPLIPLRLVVSEL
                     DAERAPTLMVLEAEPLEAPPPAGFRWVEMGAVRGRVGPSGVGGTLDWWLERGRDGPDV
                     QGPWRRPAWTRPGWHDRASSWMVDRMAEAGRPAAGPPTTIHAWPLAVVLRAPAAGGPC
                     FLKCAAPVFAHEASITSTLAERTPDLVPEVIAIDPGENWLLMGDLGADIVGLGPADGW
                     GDGLASLAALQLAWSGEPDALIRAGLPRRPLTRLAADIPGFLGQNELGDRLRPAVRDE
                     WDAAVPRLVEACERLGALGVPDTVVHGDCHPWNVARTGRGPVVFDWSDASIGPPFLDL
                     PATLARTPDVAIRRALADRYLAAWPEVAEADRPRVADLAMVVGSLSQVQSYLGFLGTI
                     DPIESLDLAGADVSWLGRSLESLEHGIAAGRRTDLLA"
     gene            complement(1316..>2056)
                     /locus_tag="AC162A_04640"
     CDS             complement(1316..>2056)
                     /locus_tag="AC162A_04640"
                     /inference="COORDINATES: protein motif:HMM:NF028222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR5010161.1"
                     /translation="ADGSPVLAYLSIGTMATAQVVPATAVVPMPDGVPPEVAALIGCG
                     VSTGVGAVIKTAEVPAGSTVAVIGLGGVGLSCVMGAVLAGARRIIAVDVNRAKIDLAG
                     ELGATHWVHADPKSPDATIEGIRVAAGEGGPDYVFEAIGLPATIEQAVAALPPGGTAV
                     LVGLTAFGQTASFEAFPFVDGGRRIIGSNYGSAVAAIDFPRYAEWYLAGRLPIDRLID
                     RRLPLSELEDAFDRLRAGEAARQVVTFD"
BASE COUNT          249 a          798 c          737 g          272 t
ORIGIN      
        1 cgacgcgcca tggaggctgc cgtcggtcaa tctcgtgatg gacgacgacc agccgatgag
       61 ccacgtgttc gaggccgccg cagccgctgt cggcctgccg ctgatcccgc tccggctcgt
      121 ggtgagcgag ctcgacgccg aacgagcgcc gacgctgatg gtcctggagg ccgagccgct
      181 cgaggcgccg ccgccggctg gcttccgctg ggtggaaatg ggcgccgtcc gcggccgcgt
      241 cggcccgtcc ggcgtcggcg ggaccctgga ctggtggctc gagcgcggtc gcgacggtcc
      301 ggacgtccag ggaccatggc gccgccccgc ctggacccgg cccggctggc acgatcgcgc
      361 gtcatcgtgg atggtcgacc ggatggccga ggccggccgg ccggcggcgg ggccacccac
      421 gacgatccac gcgtggccac tcgcggtcgt gctccgcgcc cccgcggccg gtggcccgtg
      481 cttcctcaag tgcgccgcgc cggtgttcgc gcacgaggcg tcgatcacca gcaccctcgc
      541 cgagcgcacg cccgacctcg tgccagaggt catcgccatc gatcctggcg agaactggct
      601 cctgatgggc gacctcggcg ccgacatcgt cggtctcggg ccggccgacg ggtggggtga
      661 cgggcttgcg tccctcgcgg cgctgcagct ggcctggtcc ggcgaaccgg acgccctgat
      721 ccgtgccggg ctgccgcggc gtccactgac tcgtctcgcc gcggacatcc ccggcttcct
      781 cggccagaac gagctcggcg atcgcctccg gccggcggtc cgcgacgagt gggacgccgc
      841 cgtgccgcgc ctcgtcgagg cctgtgagcg cctcggggcg ctgggcgtgc cggacacggt
      901 ggtgcacggg gactgccatc cctggaacgt cgcccggacg gggcgcgggc cggtcgtctt
      961 cgattggtcg gacgcctcca tcgggccgcc gttcctggac ctgccggcga cgctcgcacg
     1021 cacgccggac gtggcgatcc ggcgcgccct cgccgaccgg tacctcgccg cgtggcccga
     1081 ggtggccgag gccgatcggc cgcgggtcgc cgacctggcg atggtcgtcg gctccctgtc
     1141 ccaggtccag agctacctcg gcttcctcgg cacgatcgac ccgatcgaat cgttggacct
     1201 cgccggggcg gacgtgtcgt ggctgggccg gtccctcgag tccctcgagc acggcatcgc
     1261 ggccggacgc cggacggatc tgctcgcctg acgcccgtcc tgccggactc gacggtcagt
     1321 cgaacgtcac cacctggcgc gcggcctcgc cggcgcggag acggtcgaac gcgtcctcga
     1381 gctcgctgag cggcaggcgc cggtcgatga gacgatcgat gggcagccgg cccgcgaggt
     1441 accactccgc gtagcgcggg aagtcgatcg cggcgaccgc cgacccgtag ttggagccga
     1501 tgatccgccg gccaccgtcg acgaacggga acgcctcgaa gctcgccgtc tgcccgaacg
     1561 cggtcagccc cacgaggacc gccgtcccgc ccggtggcag cgcggccacc gcctgctcga
     1621 tcgtcgcggg caggccgatc gcctcgaaca cgtagtccgg cccgccctcg cccgcggcga
     1681 cccggatgcc ctcgatcgtc gcgtccggac tcttcgggtc cgcgtggacc cagtgcgtgg
     1741 ccccgagctc ccccgcgagg tcgatcttcg cgcggttcac gtccaccgcg atgatccgcc
     1801 gcgcgccggc gagcaccgcg cccatcacac acgacagccc gaccccgccg aggccgatca
     1861 ccgcgaccgt cgaaccggcc ggcacctcgg ccgtcttgat cacggccccg acgccggtcg
     1921 agaccccgca gccgatcagc gccgcgacct cgggcggcac gccgtcgggc atcgggacga
     1981 ccgccgtcgc cggcacgacc tgcgccgtgg ccatcgtgcc gatcgagagg taggcgagga
     2041 ccggcgagcc atccgc
//
LOCUS       DBNHXO010000220         5769 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2042796, whole genome shotgun sequence.
ACCESSION   DBNHXO010000220 DBNHXO010000000
VERSION     DBNHXO010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2042796"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..527)
                     /locus_tag="AC162A_04645"
     CDS             complement(288..527)
                     /locus_tag="AC162A_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010162.1"
                     /translation="MGDEPATDEGAPATPEAPDLRPRPMIERIGLGLIALVLAAMFAL
                     VSFASFVGGEPFLGVMGALGCLMIVWVGGLTLFRG"
     gene            complement(527..2029)
                     /locus_tag="AC162A_04650"
     CDS             complement(527..2029)
                     /locus_tag="AC162A_04650"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR5010163.1"
                     /translation="MSATVDRRPIGGNIWRTGVALAAAFATLAVGAGWWQVVDAQRLS
                     REPDNPAVIAVARRALRGPIVDRDGRWLARSSRDANGEATREYRDDSISPVIGYASRQ
                     FGTAGLERSYNAELIGLGGSDAFGGLLDKFDASRRTPLGLRTTLDLRLQRAAVEALGA
                     DRGAVVMLDPTTGDVLALASTPTYDAAAIADPDTAARAFGALRDDESDPLLPRATLGR
                     YVPGSVFKVVTAIAGLNTGSIEPGTTFEEQPEAEDKGLLVEGYRIRDGHHEQTDGTPL
                     DLVGATEASCNIWYALAGLATGGDDLVGQAGLLGFGAPIPFDLPTAISRVTGGGGRAP
                     GGFTDDVELASASFGQGETFVTPLQMALVASTVANDGVLMRPRLVAALTGEGPGTREI
                     APSTWRRVMASSDAQSVQAAMEAAVEGDLGRQFTAGAAVPGVPTAGKSGTAELGGKGE
                     PHSWFIGFAPVENPRVAIAVLVEQGGRGGERAAPLAGSMMERYFEQYGAP"
     gene            complement(2026..3507)
                     /locus_tag="AC162A_04655"
     CDS             complement(2026..3507)
                     /locus_tag="AC162A_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR5010164.1"
                     /translation="MTAFAGARQAARWRLHPRARHTELALLALVATALLAGGASLGAG
                     LSAQRAIRAGEPAGAIDLMPPDLRGLLVYLVALFVVHLAFVLAGRRMDQVLLPATALL
                     GGIGLLLMQRLPQGLVVQQFGEAVLGLGQLQLGWLLLALAVIAGLALAVRSDAWLRTY
                     KYTWAAAGIALLLLTFVFGSDVNGARLTLSIGPLSGQPSELLKVILVVFLAGYLSENR
                     SLLADESTRIGPIRLPPIPYLAPMVAMWAIALGIVIVQRDLGAALLFFAVFLAMLYVA
                     TGRPSYVIGGLILFIAGSWVLYTLFGHVQQRVDNWIDPFRDPLGAGYQTVQALYAFAR
                     GGLLGVGLGAGLPTIGGNLPIPAVHTDYPLAALGEELGLIGLLAILGLYLVVVERGLR
                     IAAAAHDEFRALLAAGLSLVIAVQAFIIAAGNLKLIPLTGITLPLISYGGSSLLANAV
                     VIGLLLALSDPGAEPPSVPRTGRRRLPALRRSSPVMPATGYEP"
     gene            complement(3504..3938)
                     /locus_tag="AC162A_04660"
     CDS             complement(3504..3938)
                     /locus_tag="AC162A_04660"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR5010165.1"
                     /translation="MDGFVLTLWLIRILFLVLLYAFLFAVARILMRDLRSASRGPYEL
                     GRLVVLASPHAEPPVGTSFPLDAVTTLGRDVNNTIVIEDPFASADHSVLTFRGRAWYV
                     EDLASTNGTFVNGVPVDRVAPLGFGDELQVGEVRLRLDRGRE"
     gene            complement(3940..4770)
                     /locus_tag="AC162A_04665"
     CDS             complement(3940..4770)
                     /locus_tag="AC162A_04665"
                     /inference="COORDINATES: protein motif:HMM:NF023818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HIR5010166.1"
                     /translation="MASFADVERFFERVFERSSATLFRTRIQAVQLEHRVERAMERAR
                     AHQGSRTVVPARYRVGLNPDDLAALAPESANAVALASRLADAALAFARAHAYYLPARP
                     VVVLVADPSLARGQVEVDVGPVQPASPGVEPASVAPVVAQVAVAAATDASPLEVVAEG
                     IRGDGTQTFVFRRPAPPPARALIRVCAPDGTERTIEVDGTPIAIGRAQDNRLVLLDRR
                     VSRLHGRLQARRGTLVYTDLGSTNGSRVNGIRVDEIALGLGDRVQVGDTVLVVETLPG
                     "
     gene            5008..>5769
                     /locus_tag="AC162A_04670"
     CDS             5008..>5769
                     /locus_tag="AC162A_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010167.1"
                     /translation="MTEILTESFCERCGTRYTFESSAPRTSRIGRVRTFSKGVKNFVM
                     SDEASFSEAMADARGEEELATTSHQLDAFHKTFNFCLTCRQYTCGDCWNVTDGRCLTC
                     APMDGPAELTFAAPAAAMAAGAAAATNGHAAPPADSDLWPEADLSQERLARALGDAPP
                     YAEEAAQDEVASQFEVDAAAQALDAPEAVDVGEAPTERPMSVDEAWLNAAAAAQLDEE
                     LAAADTVDIAGAGPAAAEVPAVDASAPAAELDLMAD"
BASE COUNT          913 a         2033 c         2092 g          731 t
ORIGIN      
        1 aggacggtgt cgccgacctg ctggatgttg ctcatcggac tcccgtggtg tgcggcgcga
       61 cgccgcgggc tgcgcgtgtc cggcgtgaac gtgccggtcg ggcggtgcga tgacgtcgcg
      121 ctgagcggga ttggggcgcg agcgacgtcg tccctctcaa tgcagtgtac gggtgactgt
      181 cgatccgggt tctcgcgggg cctgtctcta tagggcgtcg cagacggcta aaaggtttca
      241 tctttcccgt cctgcttcct taatgacggt ctgggccgcc gaatcgttca acctcgaaac
      301 agggtgaggc cgccgaccca gacgatcatc aggcagccga gcgcgcccat cacgccgagg
      361 aacggctcac cgcccacgaa cgaggcgaac gacacgaggg cgaacatggc cgcgaggacc
      421 agcgcgatca acccgaggcc gatccgctcg atcatcgggc gcgggcgaag gtccggggcc
      481 tccggcgtcg ccggcgctcc ctcgtcggtc gcgggctcat cacccatcac ggcgctccgt
      541 actgctcgaa gtagcgctcc atcatcgacc ctgccagcgg ggcggctcgt tcgccgcctc
      601 ggccgccctg ctccacgagc acggcgatcg cgactcgtgg gttctcgacc ggcgcgaagc
      661 cgatgaacca tgaatgtggc tcgcccttgc cgccgagctc cgccgtcccg gacttgcccg
      721 ccgtcggcac gccgggcacc gcagccccgg ccgtgaactg tcgcccgagg tcgccctcaa
      781 ccgccgcctc catcgccgcc tggacggact gggcgtcgga cgacgccatc acccgtcgcc
      841 aggtcgacgg tgcgatctcg cgcgtgcccg gtccctcacc ggtgagcgcg gcaacgagcc
      901 gcggacgcat caggacgccg tcgttggcga cggtcgaggc caccagcgcc atctggagcg
      961 gcgtcacgaa cgtctcgccc tgcccgaacg atgccgaggc cagctcgacg tcgtcggtga
     1021 atccccccgg tgcccggcct cccccacccg tcacgcggct gatcgccgtc ggcaggtcga
     1081 aggggatcgg cgcgccgaac ccgagcaggc ccgcctgccc gacgaggtcg tcgccgccgg
     1141 tcgcgaggcc ggcgagcgcg taccagatgt tgcagctcgc ctcggtggcg ccgacgaggt
     1201 ccaggggcgt gccgtcggtc tgctcgtggt ggccgtcgcg gatccggtag ccctcaacga
     1261 gcaggccctt gtcctcggcc tcgggctgct cctcgaacgt ggtgccaggc tcgatcgacc
     1321 cggtgttgag ccccgcgatc gcggtgacga ccttgaacac cgaacctggc acgtagcggc
     1381 ccaacgtggc gcgcggcagc agcgggtccg actcgtcgtc gcgcagcgcc ccaaacgcgc
     1441 gggcggccgt gtcgggatcg gcgatggcgg ccgcgtcgta cgtcggagtc gaggccagcg
     1501 ccaggacgtc accggtggtc gggtcgagca tcaccacggc gccccgatcc gcgcccagcg
     1561 cctcgacggc ggctcgctgc aggcgcaggt cgagggtcgt ccgcaggccg agcggcgtcc
     1621 gtcgcgacgc gtcgaacttg tcgagcagcc cgccaaaggc atcggaccca ccgagcccga
     1681 tcagctcggc gttgtaggaa cgctcgagac ccgccgtccc gaactggcgc gacgcgtagc
     1741 cgatgaccgg gctgatggag tcatcgcgat actcgcgggt cgcctcgccg ttcgcgtcgc
     1801 gcgagctgcg ggccagccag cggccgtcgc gatcgacgat cggaccccgg agggctcgtc
     1861 gggccacggc gatcaccgcg ggattgtcgg gttctcgtga caggcgctgg gcatccacca
     1921 cctgccacca gcccgcgccc accgccaggg tcgcgaacgc ggccgcaagg gcaacgccgg
     1981 tgcgccagat gttgccgccg atcgggcggc ggtccacggt cgcgctcacg gctcgtaccc
     2041 ggtagccggc atcaccgggg acgatcgccg cagcgccggg aggcggcggc gcccggtgcg
     2101 cgggaccgag ggcggctcgg cgcctggatc ggacagggcg agcaggagcc cgatgaccac
     2161 cgcgttggcg agcagcgagg acccgccgta ggagatcagg ggcagcgtga tccccgtcag
     2221 cgggatcagc ttgaggttgc ccgcggcgat gatgaacgcc tggacggcga tcaccagcga
     2281 gaggcctgcg gcgagcaggg cgcggaactc gtcatgggcg gcggccgcga tccggagccc
     2341 acgctcgacc acgacgagat acaggccgag gatcgccagg aggccgatga gtccgagctc
     2401 ctcgcccagc gccgcgagcg ggtagtccgt gtggacggcc gggatgggca ggttgccccc
     2461 gatcgtcggc aggcccgcgc cgagcccgac cccgagcagc ccgccgcgcg cgaaggcgta
     2521 cagggcctgc accgtctgat agccggcgcc gagcgggtcg cggaacgggt cgatccagtt
     2581 gtcgacccgc tgttgcacgt ggccgaacag ggtgtacagg acccacgacc cggcgatgaa
     2641 caggatcagg ccaccgatga cgtaggacgg gcgccccgtc gccacgtaca gcatggcgag
     2701 gaacacggca aagaacagga gggcggcacc gaggtcgcgc tggacgatga cgatcccgag
     2761 ggcgattgcc cacatcgcga ccatcggcgc gaggtacggg atcggcggca ggcggatcgg
     2821 cccgatgcgg gtgctctcgt cggccaggag ggagcggttc tccgagaggt agccggcgag
     2881 gaagacgacg aggatcacct tcagcagctc cgagggctgg ccggacagcg ggccaatgga
     2941 cagcgtgagg cgggcgccgt tgacgtcgct gccgaagacg aaggtcagga gcagcagggc
     3001 gatgccggcc gccgcccagg tgtacttgta ggtccgcaac caggcgtcgg aacgcacagc
     3061 gagggcaagg cctgcgatca cggccagcgc gagcaggagc cagccgagct ggagctgccc
     3121 aagacccagg accgcctcgc cgaactgctg gacgacgagg ccctggggga gccgctgcat
     3181 cagcagcagc ccgatcccgc cgagcagcgc cgtggctggg agcagcacct ggtccatccg
     3241 ccggcccgcg agcacgaacg cgaggtggac gacgaacagg gcgaccaggt agacgagcag
     3301 cccgcggagg tcgggcggca tcaggtcgat cgcgcccgcg ggctcgcccg cgcggatcgc
     3361 gcgctgggcg ctcaggccgg ccccgagcga ggcgccgccc gccagcagcg ccgtcgcaac
     3421 gagcgccaat agcgcaagct cggtgtggcg agctcgtggg tgaaggcgcc accgtgcggc
     3481 ctgacgggca ccggcgaacg cggtcactcc cgaccccggt cgagacgcaa tcggacctcg
     3541 cccacctgga gctcatcgcc gaagccgagc ggtgcgacgc ggtccacggg cacgccgttc
     3601 acgaaggtgc cgttggtgct cgcgaggtcc tcgacgtacc aggctcgccc tcggaacgtg
     3661 agcaccgagt gatcggccga cgcgaacggg tcctcgatca cgatggtgtt gttgacgtcc
     3721 cgcccgagcg tcgtgacggc gtccagcggg aacgacgtcc cgacgggcgg ttcagcgtgc
     3781 ggtgacgcga gcaccaccag gcggcccagc tcgtacggcc cacggctcgc gctgcggagg
     3841 tcgcgcatga ggatgcgggc gacagcgaac aggaatgcgt acagcaggac caggaacagg
     3901 atgcggatga gccacagcgt caggacgaac ccgtccacgt caaccaggaa gcgtctcgac
     3961 gacgagcacc gtgtcgccga cctggacgcg atcgcccagg cccagcgcga tctcatccac
     4021 gcggatcccg ttgacccgac tgccgttggt gctgccgagg tcggtgtaca cgagcgttcc
     4081 gcgacgcgcc tggagccgtc catgcagccg cgacacgcga cgatcgagca gcacgagccg
     4141 attgtcctgc gcccggccga tcgcgatcgg cgtgccgtcc acctcgatcg ttcgctcggt
     4201 gccgtcgggc gcgcagaccc ggatcagcgc acgggccggc gggggtgcgg gccgccggaa
     4261 aacgaacgtc tgggtgccgt cccctcggat gccctcggcc accacctcga gtggcgacgc
     4321 gtcggtggca gcggcgaccg cgacctgggc gacgaccggg gcgacggatg ccggctccac
     4381 gccaggcgac gcgggctgga ccgggccgac atcgacctcg acctggccgc gggcgaggga
     4441 cggatctgcg accaggacga caaccggccg cgcgggcagg tagtaggcat gcgcccgcgc
     4501 gaacgccagt gcagcgtccg ccagacgcga tgcgagcgcg acggcgttgg ctgactcggg
     4561 agcgagcgcg gccaggtcgt cggggttgag tccgacccgg tagcgggcgg ggaccacggt
     4621 ccgcgagccc tgatgggcac gcgcgcgctc catcgcccgc tcgacccgat gctcgagctg
     4681 gaccgcctgg atgcgggtcc ggaacagcgt ggcgctggag cgctcgaaga cccgctcgaa
     4741 gaatcgttcg acgtcggcaa aggaggccat gtggacggga accgtaccag agcctcgtgc
     4801 tgcacccccg atgcgagccg acgcattggg cggcagggcg ctgcgacatg gtcgcgcccc
     4861 cattttggcg ccgggtggtg cgcgggacca acggcggtta gtcccggtac ctcggtctgg
     4921 ggggtccgtt gagcacgtgg ttatactctt ggcccctgcc cgaccggact agatcgggcg
     4981 tctgaacacg tctgcccggg aacctgcatg acggaaatcc tcaccgagtc gttctgcgaa
     5041 cggtgcggca cccgctacac cttcgaatcg agcgcaccgc gaacgtcgcg gatcgggcgc
     5101 gtccggacct tctccaaggg cgtgaagaac ttcgtcatgt cggacgaggc ttcgttctcg
     5161 gaggcgatgg cggacgcccg cggcgaggag gagctggcga cgacctcgca ccagctcgac
     5221 gcgttccaca agacgttcaa cttctgcctg acctgccggc agtacacctg cggcgactgc
     5281 tggaacgtga ccgatggcag gtgcctcacc tgtgcgccca tggacggccc ggcggagctg
     5341 acgttcgccg cgccggcggc cgcgatggcg gctggtgccg ccgctgccac caacgggcac
     5401 gccgctcccc cggccgacag cgacctgtgg cccgaggccg acctctcgca ggagcgcctc
     5461 gcgcgcgccc ttggcgatgc gccgccctac gccgaagagg ccgcgcagga cgaggtcgcg
     5521 tcccaattcg aggtcgacgc cgccgcgcag gcactcgacg cgcccgaggc cgtcgacgtc
     5581 ggggaggccc cgaccgagcg cccgatgtcc gtcgacgagg catggctgaa cgccgccgcg
     5641 gcagcgcagc tcgacgaaga actcgcagcg gccgataccg tcgacatcgc gggcgcaggg
     5701 cctgccgccg ccgaggttcc cgccgtggac gcgtctgcgc cggccgccga gctcgacctc
     5761 atggccgac
//
LOCUS       DBNHXO010000221         3989 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5417378, whole genome shotgun sequence.
ACCESSION   DBNHXO010000221 DBNHXO010000000
VERSION     DBNHXO010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5417378"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="AC162A_04675"
     CDS             <1..243
                     /locus_tag="AC162A_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HIR5010168.1"
                     /translation="NGWGAKEPAWWLNLQAHPDTTVELRDGLRAVHARAALGEERERL
                     WARWRALNDQYDAWAALRSRPTAVVVLEPRAGGAAG"
     gene            376..1401
                     /locus_tag="AC162A_04680"
     CDS             376..1401
                     /locus_tag="AC162A_04680"
                     /EC_number="3.5.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014442300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008660 -
                     1-aminocyclopropane-1-carboxylate deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009310 - amine catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate deaminase"
                     /protein_id="HIR5010169.1"
                     /translation="MSLADFPRTPLLFGPSPIQRLDRLSKHLGGATVWAKREDCNSGL
                     AFGGNKVRKLEYLLADALAQSCDTLVSIGGVQSNHTRQVAAVAAHAGLKCVLVQEHWV
                     EWPDVTYDRTGNILMSRLMGADVRLDPAGFGIGFKASWEQALADVIAAGGKPYAIPAG
                     ASDHPLGGHGFAGWALEVAEQERELGVFFDTIVVCSVTGSTQAGMIAGFAVLEDAGGT
                     PRRVVGVDGSATPAETRDQVARIARRTAAAIGLGRDLREDEIILDDRYHAGVYGIPDE
                     ATLEAMRLVARLEGMITDPVYEGKSMAGMVDMVARGEIARDTNVLYAHLGGQPALNAY
                     ASLFTNG"
     gene            complement(1454..2092)
                     /locus_tag="AC162A_04685"
     CDS             complement(1454..2092)
                     /locus_tag="AC162A_04685"
                     /inference="COORDINATES: protein motif:HMM:NF025255.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM7S3/TM198-like domain-containing protein"
                     /protein_id="HIR5010170.1"
                     /translation="MQLLMAILAVVIGLAFCFGGWRFFLLLLPIWGFVVGFGLGTEMM
                     RNLFGDGTFATITSWIVGFVIAVVFAVLSYLYYYAAIAILAGTVGYALGASAWGIIGN
                     EQGVIAFVIGLAVGVVFAIAVLALNVPRYLVIVLTGLGGAAGFLEGWFLLIGKIPTDN
                     VTWFSVGALIKDSWFYLIVWALIAAAGILAQLRAPALGPDTYELDSTSYRYS"
     gene            complement(2163..3986)
                     /locus_tag="AC162A_04690"
     CDS             complement(2163..3986)
                     /locus_tag="AC162A_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR5010171.1"
                     /translation="MQCVRTSARSRRPGMGAVPYAGGTTFRVWAPHADAVFVTGTFDA
                     WAGDRIALGRDSDGTTGTWSTDVAGVGPGAEYRYTIRTPTGDLSRIDPYARQVTNSVG
                     NAVVYDPAGFDWGDDTFETPTWDDLVIYELHVGTFSAKDDRRGTFDTARRRLGYLSEL
                     GVSAVQVMPPLEFAGNISWGYNPAHLFAIESGYGGPDAFKRFVRDAHAHGIGVIVDVV
                     YNHFGPSDLDLWRFDGWSEDDGGGIYLYNDERAVTPWGNTRPDYGRGEVRTFLRDSAM
                     TWLEEFHCDGLRFDSTINIRTVNGDPNDDGSALDDGWSFMAWINDEIRARQPWKITIA
                     EDMQDDPALVAPTDEGGAGFGAQWDGGFVHRVRGAVVASEDGARDMDEVVTAILGEGR
                     GASLARVIYTESHDEVANGRTRVPEAIAPGDADNWWARKRAVLGSALVLTSPGIPMLF
                     QGQELLEDRWFDDQVGLDWAKASSNHGVLRFHRDLIALRRAADGATLGLRGSNVAILQ
                     ADNDAKLLAMHRWMDGGPHDDTVVIANFADRTVEDWRIGLPAPGRWAVRLNSDSAVYA
                     PDFGGHEANDLNADGPPLDGCAQSGLVAVGPYSVVVLSREA"
BASE COUNT          617 a         1443 c         1368 g          561 t
ORIGIN      
        1 aacggctggg gggcgaagga gcccgcctgg tggctgaacc tccaggcaca tcccgacacg
       61 acggtcgagc tcagggatgg tctgcgtgcc gtccacgctc gcgcagcgct gggcgaggag
      121 cgcgagcggc tctgggcgag gtggcgggcg ctgaacgacc agtacgacgc ctgggcggcg
      181 ctgcgttcga gaccgacggc ggtcgtcgtg ctcgagcccc gcgccggcgg ggccgccggg
      241 tagcggccgc cgaccgggtc cagatctgat gccggtggcg ggttccccgt cgccggcatc
      301 cgtgcttgtg cgggcatctg tgaggcccta ccgtggcgag gcccctccac acagcccagc
      361 cccgggagcc tgcccgtgtc cctcgccgac tttccccgga ccccactcct gtttggcccg
      421 tcgcccatcc agcgacttga ccgcctctcg aagcatcttg gcggggcgac ggtgtgggcc
      481 aagcgcgagg actgcaacag cggcctcgcc ttcggtggca acaaggtccg caagctcgaa
      541 tacctcctcg ccgacgcatt ggcccagagc tgcgacacgc tcgtgtccat cggcggggtc
      601 cagtcgaacc acacccgcca ggtcgccgcc gtcgctgcgc acgccgggct gaagtgcgtc
      661 ctcgtccagg agcactgggt ggagtggccc gacgtcacct acgaccgcac gggcaacatc
      721 ctcatgagcc gcctgatggg cgcggacgtg cgtctcgacc cggccggctt cgggatcggg
      781 ttcaaggcga gctgggagca ggcgctcgcg gatgtgatcg cggccggcgg caagccctac
      841 gcgatccccg ccggtgcctc ggaccatccg ctcggcgggc acggcttcgc cggctgggcg
      901 ctggaggttg ccgagcagga gcgggagctg ggcgtcttct tcgacaccat cgtcgtgtgc
      961 tccgtcacgg gctcgacgca ggccgggatg atcgccgggt tcgccgtcct ggaggacgcc
     1021 ggcggcaccc cgcggcgtgt cgtgggcgtg gacggatcag cgacgcctgc cgagacccgc
     1081 gaccaggtcg cgcggatcgc acgccggacc gccgccgcca tcggcctcgg acgcgacctg
     1141 cgcgaggacg agatcatcct ggacgaccgc taccacgccg gcgtgtacgg catcccggac
     1201 gaggcgacgc tcgaggccat gcgccttgtc gcacgcctgg agggcatgat cacggacccc
     1261 gtgtacgagg ggaagtccat ggcgggcatg gtcgacatgg tggcgcgcgg cgagatcgcc
     1321 agggacacga acgtcctcta cgcacacctc ggcgggcagc cggcgctcaa cgcgtacgcc
     1381 agtctgttca cgaacggctg acccctgggc cggggccggg ggacccaccg atggtgagtc
     1441 ccccgacgga ggctcagctg tagcggtacg aggtgctgtc gagctcgtac gtgtccgggc
     1501 caagcgccgg ggcccgcagc tgcgcgagga tcccggccgc cgcgatcagc gcccacacga
     1561 tgaggtagaa ccagctgtcc ttgatcaggg ccccgaccga gaaccaggtc acgttgtccg
     1621 tcgggatctt gccgatgaga aggaaccacc cctcgaggaa cccggcggcg ccgccgagcc
     1681 cggtcagcac gatgacgagg tagcgcggca cgttcaacgc cagcacggcg atcgcgaaca
     1741 ccacgccgac cgcgagcccg atcacgaacg cgatcacgcc ctgctcgttc ccgatgatcc
     1801 cccacgcgct cgcgccgagg gcgtacccga ccgtccccgc gaggatcgcg atggctgcgt
     1861 agtagtagag gtacgacagc acggcgaaca cgaccgcgat gacgaacccg acgatccagc
     1921 tggtgatcgt ggcaaaggtc ccgtcgccga acaggttgcg catcatctcc gtgcccagcc
     1981 cgaagcccac gacgaagccc caaatcggca gcagcagcag gaagaatctc cacccgccga
     2041 aacagaacgc caggccgatc accacggcca gaatggccat cagcagttgc attccacgcc
     2101 tccctatgcg ccgtctctcc atcggcgacg cgccgagcat actgcgcgct cggtcgcccg
     2161 attcacgcct cgcgcgacag gaccaccacg ctgtaggggc caacggccac gagaccgctc
     2221 tgcgcgcagc cgtccagcgg cggcccgtcc gcgttgaggt cgttcgcctc gtggccgccg
     2281 aagtccggcg cgtagacagc agaatccgag ttcagccgca cggcccaccg accgggcgcc
     2341 gggagcccga tccgccagtc ctcgaccgtc cgatcggcga agttcgcgat gaccacggtg
     2401 tcgtcgtgcg gccccccgtc catccagcgg tgcatcgcga gcagcttggc gtcgttgtcg
     2461 gcctgcagga tcgccacgtt cgagccccgc aggcccagcg tggcgccgtc cgcggcgcgc
     2521 cggagcgcga tgaggtcgcg gtggaagcgc aggaccccgt ggttcgagct cgccttggcc
     2581 cagtccaggc cgacctgatc gtcgaaccag cggtcctcga gcagctcctg gccctggaac
     2641 agcatcggga tgccgggcga ggtcagcacg agcgccgacc cgaggacggc acgcttcctc
     2701 gcccaccagt tgtcggcgtc accaggggcg atcgcctccg ggacacgcgt ccggccgttg
     2761 gcgacctcgt cgtgcgactc ggtgtagatc acgcgcgcca gcgacgcccc ccgaccctcg
     2821 ccgaggatcg cggtgacgac ctcgtccatg tcgcgcgccc cgtcctcgga cgcgacgacc
     2881 gccccgcgca cgcggtggac gaacccgccg tcccattggg cgccgaagcc ggcgccgccc
     2941 tcgtcagtcg gtgcgaccag ggccggatcg tcctgcatgt cctcggcgat cgtgatcttc
     3001 cacggctggc gggcccggat ctcgtcgttg atccaggcca tgaacgacca cccgtcatcg
     3061 agcgccgaac cgtcgtcgtt cggatcgccg ttcaccgtcc ggatgttgat ggtcgagtcg
     3121 aagcgcagcc cgtcgcagtg gaactcctcg agccaggtca tggcgctgtc gcgcaggaac
     3181 gtgcggacct cgccgcgccc gtagtcgggg cgcgtattcc cccagggcgt caccgcccgc
     3241 tcgtcgttgt agaggtagat gccgcccccg tcgtcctcgg accagccgtc gaaccgccac
     3301 aggtcgaggt cggacggccc gaagtggttg tagacgacgt cgacgatcac gccgatcccg
     3361 tgcgcgtggg cgtcgcggac gaagcgcttg aacgcgtcgg gcccgccgta gcccgactcg
     3421 atggcgaaca ggtgggccgg gttgtagccc caggagatgt tgcccgcgaa ctcgagcggc
     3481 ggcatgacct ggacggccga gacgccgagc tcgctcaggt agccgagccg ccggcgggcg
     3541 gtatcgaacg tgcctcgtcg atcgtccttc gcggagaacg tcccgacgtg gagctcgtag
     3601 atgacgaggt cgtcccacgt cggcgtctcg aaggtgtcgt cgccccagtc gaacccggcc
     3661 ggatcgtaga cgacggcgtt ccccacggaa ttcgtcacct ggcgcgcgta cgggtcgatg
     3721 cgcgacaggt cgcccgtcgg cgtgcggatc gtgtagcggt actcggcgcc gggcccgacg
     3781 cccgcgacgt ccgtcgacca ggtcccggtc gtcccgtcac tgtcccgccc gagggcgatc
     3841 cggtcgccag cccacgcatc gaacgtgccg gtgacgaaga cggcgtcggc gtggggtgcc
     3901 cagacgcgga acgtggtccc acccgcgtac gggacggcgc ccatccccgg tcgtctcgag
     3961 cgcgccgaag tcctcacgca ctgcaatct
//
LOCUS       DBNHXO010000222         2991 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2638035, whole genome shotgun sequence.
ACCESSION   DBNHXO010000222 DBNHXO010000000
VERSION     DBNHXO010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2638035"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="AC162A_04695"
     CDS             complement(<1..664)
                     /locus_tag="AC162A_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR5010172.1"
                     /translation="MKTGLQISSFTWPGGPEAIGPTLGRIVHDADEAGFDSIWVMDHF
                     FQIRSVGRAEEPMLEGMTALGFMAAHSSRARIGLMVGGVHYRQAPLWVKATTTLDVLS
                     GGRAWLGIGAAWNQQESNGLGFPFPPLGERFEMLEDTLRYAHDMWSGERGTEAAFEGR
                     HVSAMHLLNSPQSISRPRPPIMVGGGGEQKTLRLVAKYADATNVFGGPETIHHKYEVL
                     RRH"
     gene            complement(690..1472)
                     /gene="cofE"
                     /locus_tag="AC162A_04700"
     CDS             complement(690..1472)
                     /gene="cofE"
                     /locus_tag="AC162A_04700"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018193304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HIR5010173.1"
                     /translation="MSTDPSDGRVEIIALAGLPEIREGDDLAAMIGDALEATSGALPV
                     RDGDVLVVTQKVVSKAEGAVVDLRTVEPRPEAVAWAEAWGRDARQIEVVLREARRIVR
                     MQNGVLITETEHGFVCANGGVDASNVGPDSGDLVTLLPRDPDGSADRIRASLRARLGR
                     DLAVIVSDSFGRPWRWGIVDVALGVSGLLPLDDLRGQPDADGRVMRTTVRATADEIAS
                     AAELALGKTAGRPVALVRGARPQPGEGRIAELVMPAENDLFR"
     gene            complement(1462..2082)
                     /gene="cofC"
                     /locus_tag="AC162A_04705"
     CDS             complement(1462..2082)
                     /gene="cofC"
                     /locus_tag="AC162A_04705"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HIR5010174.1"
                     /translation="MQTDHTRRTVVVIPVRAFEGAKSRLGAVLDAEERRDLVERLLRR
                     AVTAARETPGVTRVVVVSPDPEVLAIAKAAGALPIAQRSRGLNPALQEARTAADADRL
                     LIIPADLPAVSAGALAQVLAAGDAAGRPSVVLAPDRHGRGTNALLLDPPDVIDPAFGG
                     DSRAGHAWLASSAEAAFVEVPDVLGLDVDTPDDLLLAEATEAIGVD"
     gene            complement(2123..2449)
                     /locus_tag="AC162A_04710"
     CDS             complement(2123..2449)
                     /locus_tag="AC162A_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010175.1"
                     /translation="MIGCGLLLLVLVLGVGACTVFFVRTLGSGFAVIANSGGDIESFS
                     SSTFNGRTVITFQAARGVDLEDGPRLACEAIKPALAGSELEDEEWVLLNRAGDVIASN
                     ETACGP"
     gene            complement(2656..>2991)
                     /locus_tag="AC162A_04715"
     CDS             complement(2656..>2991)
                     /locus_tag="AC162A_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR5010176.1"
                     /translation="SAADYRHGPLALVERDYPVLAVATSGAVAADLIALLREIRDDYG
                     ADLVVLSDREDVRAIGRRSIRLPGGVPDHLAPIVSIVPAQLFAYHLTIARGLDPDAPR
                     HISKVTLTT"
BASE COUNT          409 a         1076 c         1131 g          375 t
ORIGIN      
        1 aatggcggcg caggacctcg tacttgtggt gaatcgtctc cgggccgccg aacacgttcg
       61 tggcgtcggc gtacttcgcg accaggcgca gcgtcttctg ctccccgccg cccccgacca
      121 tgatcggtgg acgcggccgg ctgatcgact ggggcgagtt cagcaggtgc atcgcgctga
      181 cgtggcggcc ctcgaacgcc gcctccgtgc cgcgctcgcc cgaccacatg tcgtgggcgt
      241 agcgcagggt gtcctcgagc atctcgaacc gctcgccgag cggcgggaac ggaaacccga
      301 gcccgttcga ctcctgctgg ttccaggccg cgccgatgcc cagccaggcg cggccgccgg
      361 acagcacgtc gagggtcgtc gtggccttga cccaaagcgg ggcttggcgg tagtgcacgc
      421 cgccgaccat gaggccgatc cgcgcccggc tcgagtgcgc ggccatgaac ccgagtgccg
      481 tcatgccctc gagcatcggc tcctcggcgc ggcccacgct ccggatctgg aagaagtgat
      541 ccatgaccca gatggagtcg aagccggcct catcggcgtc gtggacgatc cggccgaggg
      601 tgggtccgat ggcctcgggg ccgccgggcc aggtgaagga cgagatctgg agccctgtct
      661 tcatgccccg agcgtatcgc gggcgcgggc taccggaaca ggtcgttctc ggcgggcatc
      721 acgagctcgg cgatccgccc ctcgccgggt tgcggccgcg cgccgcggac cagcgcgacc
      781 ggccggccgg ccgtcttgcc cagggcaagc tcggcggcgc tcgcgatctc gtcggccgtg
      841 gcgcggaccg tcgtccgcat cacccggccg tctgcatcgg gctggccccg caggtcgtcg
      901 agcggcagga ggcccgacac cccgagcgcg acgtccacga tgccccagcg ccacggacgg
      961 ccgaagctgt cggagacgat gacggcgagg tcgcgcccga gccgcgcccg gagcgacgcc
     1021 cggatccggt cggcggaccc gtccgggtcg cgggggagca gcgtgaccag gtcgcccgag
     1081 tccgggccga cgttggacgc gtccaccccg ccgtttgcgc acacgaaccc gtgctcggtc
     1141 tcggtgatca ggaccccgtt ctgcatccgg acgatccgcc gggcctcgcg caggaccacc
     1201 tcgatctggc gggcgtcgcg accccacgcc tcggcccacg cgaccgcctc ggggcggggc
     1261 tcgaccgtcc gcaggtcgac gacggcaccc tcggccttgg acacgacctt ctgggtgacg
     1321 accagcacgt cgccgtcccg caccgggagc gcgccgctgg tcgcctcgag cgcgtcgccg
     1381 atcatcgccg cgaggtcgtc gccctcgcgg atctcgggca gcccggcgag cgcgatgatc
     1441 tccacccggc cgtcggacgg gtcagtcgac accgatcgcc tccgtggcct cggccagcag
     1501 caggtcgtcc ggcgtgtcga cgtccaggcc cagcacgtcg ggcacctcga cgaacgccgc
     1561 ctcggccgag gacgcgagcc acgcgtggcc ggcccggctg tcgccgccga acgccggatc
     1621 gatgacgtcg ggcgggtcga gcagcagcgc gttggtgccg cgtccgtggc gatcgggcgc
     1681 gagcacgacg ctcggccggc cagcggcatc gccggcggca agcacttgcg ccagggcccc
     1741 ggcgctcacc gcgggcaggt ccgccgggat gatcagcagg cgatcggcgt cggcggcggt
     1801 ccgcgcctcc tggagcgccg ggttgaggcc gcgcgaccgc tgcgcgatcg ggagcgcacc
     1861 ggcggccttg gcgatcgcga ggacctcggg gtcaggcgag accacgacga cccgggtgac
     1921 gccgggcgtc tcgcgcgccg cggtcacggc ccgccggagc agccgctcca cgaggtcgcg
     1981 gcgctcctcg gcgtcgagga cggcgcccag gcgcgacttg gcgccctcga acgcgcggac
     2041 ggggatgacg acgacggtgc gccgggtgtg gtcggtctgc acgtctcgat cgtagcgcgc
     2101 ggtccagccg cgaccgaaaa cgtcaaggcc cgcaggcggt ctcgttcgac gcgatcacgt
     2161 caccggcgcg gttgaggagc acccactcct catcctccag ctcggatccg gccagcgcgg
     2221 gcttgatggc ctcgcatgcg agacgcgggc cgtcttcgag atcgacgccg cgcgccgcct
     2281 ggaaggtgat cacggtgcgc ccattgaagg tcgaggacga gaagctctcg atgtcgccgc
     2341 ccgagttcgc gatcaccgcg aagccagagc cgagcgtcct gacgaagaac acggtgcagg
     2401 cgccgacccc gaggacgagc accagcagca gcaggccgca cccgatcacc cagcggcggc
     2461 gcgagcttcg gggtggagtc gccgacgggg gcgcccagga cgccgggccc ggcgacggac
     2521 cgacgtaggg gggcgccgac ggcggtaggc ccggcggtgg tgccgacggc ggcggaggcg
     2581 ccggcggaat gggcggtgtg ggaggatcgg cctgcatcgc gtgatcctac ggtccggcgc
     2641 gggtcgcgcg tcaggtcagg tggtcagggt gaccttgctg atgtggcgcg gcgcgtcggg
     2701 gtcgaggccg cgggcgatcg tgaggtggta ggcgaacagc tgcgccggca cgatggatac
     2761 gatcggcgcc aggtggtccg gcacgccccc cggcagccgg atcgagcgcc ggccgatcgc
     2821 tcgcacgtcc tcgcggtcgg acaggacgac gaggtcggcc ccgtagtcgt cgcggatctc
     2881 gcgcagcagc gcgatcaggt cggcggcgac agcgccggac gtcgcgacgg cgagcaccgg
     2941 gtagtcgcgc tcgacgaggg cgagcggccc gtggcggtag tcggcggccg a
//
LOCUS       DBNHXO010000223         2827 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4623293, whole genome shotgun sequence.
ACCESSION   DBNHXO010000223 DBNHXO010000000
VERSION     DBNHXO010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4623293"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2794)
                     /gene="carB"
                     /locus_tag="AC162A_04720"
     CDS             complement(<1..2794)
                     /gene="carB"
                     /locus_tag="AC162A_04720"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR5010177.1"
                     /translation="MAEDREAFRDLLDRTGQPYAPSSIVEGPDEEARHLSAEAALAEI
                     GLPAIIRPAFTLGGTGGGIVETEDASWERVRAGMRASPIKQVMIERCLVGWQEIEYEV
                     MRDAEDTCIAVCSMENVDPLGVHTGDSIVVAPVQTLTDQVHQRLRSAALAIIRALGVE
                     GGCNVQFALSPDSTEYAVIEVNPRVSRSSALASKATGYPIARVAAQIAAGRTLADIPY
                     VVTGTTVAAFEPALDYVVVKLPRFPFDKFPTADRRLGSQMKATGEVMAIDRTFGAALN
                     KALRGLEQAGAGPLGEDPSWRPTFDYLAAVYATGDDAEDEDLVIRWIDERGLACESSR
                     FAERSAAPIVLRRFVEPSDSRLWRVLGLLRRGVAQETIREATGISPWFLAEMGRNVGL
                     EADVRAAGAAIADPDDVDGARLLATAKRAGFADRDLAALAGVGEADLLTARTALGLEP
                     GYAMVDTCAAEFAAETPYFYSTYASAGSPPEAPPVERPAALVIGSGPVRIGQGIEFDY
                     CAVQAADVLRREGWSAVMVNSNPETVSTDFDASTRLYFEPLDHESVRSIITFESGGAD
                     SAPEDTLPAVVAYGGQTPLNLAAPLVRSGVPLLGSSLETIDQAEERTRFSALLDRLGI
                     PQPEGGMAESIEEALTLAERIGYPVIVRPSFVIGGLAIDFAYSPDDLARHLAAAAIVD
                     PDRPVRIDRFLEGIEVDVDAICDGHGVLIPGLLEHVERAGVHSGDSIAVFPPQHVAEG
                     DRDLIVWTMERICLALGARGLVNAQFIVRDDGVYLIEVNPRASRTVPFMSKVTGVPMV
                     ELAVRISLGATLAELGWEAGLRPDPAFVAVKAPAFSTAKLKGVDPSVGPFMQSTGEVI
                     GIADDARVAMAKALTGASLIPPRTAETAGEAPLALLSIADRDKWGLLDLARALDVAGY
                     RLAATAGTRAALEGAGF"
BASE COUNT          395 a         1013 c         1054 g          365 t
ORIGIN      
        1 cgaagccggc cccctccagc gccgcgcgcg tcccggccgt cgccgccagc cggtagccgg
       61 cgacgtccag cgctcgggcg aggtccagca ggccccactt gtcacggtcc gcgatcgaga
      121 gcagcgcgag cggggcctcg cctgcggtct ccgccgtgcg cggcgggatc agcgacgcgc
      181 ccgtgagcgc cttggccatc gccacccgcg cgtcgtcggc gatcccgatg acctcgcccg
      241 tcgactgcat gaacggcccg acggacgggt cgacgccctt gagcttggcg gtggagaagg
      301 ccggtgcctt gaccgcgacg aaggcgggat cgggccgcag gccggcctcc cagccgagct
      361 cggccagcgt cgcgccgagc gagatgcgca cggccagctc gaccatcggc acgcccgtca
      421 ccttggacat gaacggcacg gtccgcgatg cccgcgggtt gacctcgatc aggtacacgc
      481 cgtcgtcgcg cacgatgaac tgcgcgttga cgagaccgcg cgcccccagc gcgaggcaga
      541 tccgctccat cgtccacacg atcaggtcgc gatcgccctc ggcgacgtgc tggggcggga
      601 acaccgcgat cgagtcgccc gaatggacgc cggcgcgctc gacgtgctcg agcaggccgg
      661 gaatgagcac gccatgcccg tcgcagatgg cgtcgacgtc gacctcgatg ccctcgagga
      721 agcggtcgat gcgcaccggc cggtcgggat cgacgatggc ggcggccgcc aggtgccggg
      781 cgaggtcgtc gggcgagtag gcgaagtcga tggcgaggcc gccgatgacg aacgacgggc
      841 ggacgatgac cggatagccg atccgctcgg ccagcgtgag cgcctcctcg atggactcgg
      901 ccatgccgcc ctcgggctgg gggatgccca ggcggtcgag cagcgcggag aaccgggtcc
      961 gctcctcggc ctggtcgatg gtctcgaggc tggacccaag caggggcacg cccgagcgga
     1021 cgagcggcgc ggcgaggttg agcggcgtct ggccgccgta cgcgacgacg gccgggagcg
     1081 tgtcctcggg ggccgagtcg gcgccgccgc tctcgaacgt gatgatgctg cgcacgctct
     1141 cgtggtcgag cggctcgaag tacaggcgcg tcgacgcgtc gaagtcggtc gagacggtct
     1201 cggggttgga gttgaccatg accgcgctcc agccctcgcg ccggagcacg tcggccgcct
     1261 ggaccgcgca gtagtcgaac tcgatcccct gcccgatccg gaccgggccg ctgccgatga
     1321 cgagggcggc ggggcgctcg accggcggag cctcgggcgg cgaaccggcc gaggcatagg
     1381 tcgagtagaa gtacggcgtc tcggccgcga actccgcggc gcaggtgtcg accatcgcgt
     1441 agccgggttc caggcccagc gcggtccgcg ccgtgaggag gtccgcctcg cccaccccgg
     1501 ccagcgcggc caggtcgcgg tcggcgaagc cggcacgctt ggccgtcgcg aggaggcggg
     1561 cgccgtccac gtcgtccggg tcggcgatgg cggcaccggc cgcgcgcacg tccgcctcga
     1621 gcccgacgtt gcggcccatc tcggcgagga accagggcga gatgcccgtt gcctcgcgga
     1681 tcgtctcctg ggctacgcca cgcctcagca gcccgaggac ccgccacagc cgcgagtcgg
     1741 acggttcgac gaaccggcgc aggacgatcg gggcggccga gcgctcggcg aatcgcgagc
     1801 tctcgcaggc gaggccgcgc tcgtcgatcc agcggatgac caggtcctcg tcttccgcgt
     1861 cgtcgccggt cgcgtacacg gccgcgaggt agtcgaaggt cggccgccag gaggggtcct
     1921 cgcccagcgg tcccgcgccc gcctgctcga ggccccgcag cgccttgttc agcgccgccc
     1981 cgaaggtccg gtcgatcgcc atcacctcgc cggtcgcctt catctgcgac ccgagccggc
     2041 ggtccgccgt cgggaacttg tcgaacggga agcgcgggag cttgacgacg acgtagtcca
     2101 gggccggctc gaatgcggcg accgtcgtgc cggtcacgac gtaggggatg tcggcgaggg
     2161 tccggccggc ggcgatctgg gccgcgaccc gggcgatcgg gtagcccgtg gccttcgagg
     2221 cgagcgcgga ggaccgcgag acgcgggggt tgacctcgat caccgcgtac tcggtcgagt
     2281 ccggcgacag ggcgaactgg acgttgcagc cgccctcgac gccgagcgcc cggatgatcg
     2341 cgagcgccgc gctgcgcagc cgctggtgga cctggtcggt cagcgtctgg accggcgcca
     2401 cgacgatcga gtcgccggtg tggacgccca acggatccac gttctccatc gagcacacgg
     2461 cgatgcacgt gtcctcggcg tcgcgcatga cctcgtactc gatctcctgc cagccgacga
     2521 ggcagcgctc gatcatcacc tgcttgatcg ggctcgcccg catgccggcg cggacacgct
     2581 cccaggaggc gtcctcggtc tcgacgatgc cgccgccggt gccgccgagc gtgaacgcgg
     2641 gccggatgat ggcgggcagc ccgatctcgg cgagggctgc ctccgccgag aggtgccggg
     2701 cctcctcatc cgggccttcg acgatcgacg acggcgcata cggctggccg gtccggtcga
     2761 gcaggtcgcg gaacgcctcc cggtcctcgg ccatgcggat ggcctcgagc ggggtgccca
     2821 gcaggcg
//
LOCUS       DBNHXO010000224         2311 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3035506, whole genome shotgun sequence.
ACCESSION   DBNHXO010000224 DBNHXO010000000
VERSION     DBNHXO010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3035506"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="AC162A_04725"
     CDS             complement(<1..190)
                     /locus_tag="AC162A_04725"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR5010178.1"
                     /translation="MPSTRRVGIITAGGDSPGLNAAIRGFGKAAISYGMEVVGFRDGF
                     RGLAEDRRVKLDTRALAGI"
     gene            288..>2311
                     /locus_tag="AC162A_04730"
     CDS             288..>2311
                     /locus_tag="AC162A_04730"
                     /inference="COORDINATES: protein motif:HMM:NF014759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HIR5010179.1"
                     /translation="MERLLLLDGNGLIYRGYFALIEQPLTTSKGELVTAVFGFTNIVL
                     RAIQDAKPDRLGVAFDLGKPTFRHERYAEYKATRQRMPDDMREQIPKVREVVKALGIP
                     VYEREGFEADDAIATLVGQAEAADLDVTILTGDLDMLQLVSERTKLMVSLRGGIANTV
                     AYDLAKIQERWGLRPDQMLDYKALKGDPTDNIPGIPGVGEKTASKLIATWGTLDVLYE
                     HIDEVMPEKLRPLLEEYRDVVLESRELMRLVRDVDVELDLSRGRVGEYDRETVVRLFR
                     EYEFRTLIDRLPPLIGERPEDAIAAMRELREAGFPAAQGVGRGAGGGRPGGQGTAPVD
                     PGSLQLSMDFDVVSGGGGGSATGGIAATSPAVEARAEAIAAASGDVPGALAAAIVDPG
                     RIEILDAAGVVGLEPWLRDQAAVGVAIVVDDPRPMAGTLLALAVAGEDGRVVAADGPE
                     ASDALRHLLERLDARLVGHEVKPLLTARFAEDPSAAPVPIAFDTQIAAYLVNAALRAQ
                     KIADVVAERLDLVLPPAAAGLPPTATAGLEALSAVAVRPSLEQAMRDEGVDRLFEEIE
                     LPLIPILARMEAAGIALDMAALAELEREFAAEIARLEGEIYAAVGHQFTIGSPKQLGD
                     ILFVELGLPKGRKTKTGYSTDASVLEELRGVHPVIEPVLEWRIYTKLRST"
BASE COUNT          331 a          817 c          846 g          317 t
ORIGIN      
        1 ggatcccggc cagtgcccgg gtgtcgagct tgaccctgcg gtcctcggcc agcccccgga
       61 agccgtcgcg gaacccgacg acctccatgc cgtagctgat ggcggccttg ccgaaccccc
      121 ggatcgccgc gttgaggccg ggactgtcgc caccggcggt gatgatcccg acgcggcgcg
      181 ttgagggcat gcccagatcg tagcggctcg ccggtggtcc atccatgaca ggtccgggtc
      241 gtgccgcggg tacactttcg atgtccccac ccgcacgctc ggagccgatg gaacgcctcc
      301 tgctgctcga cggcaacggg ctcatctacc gcggctactt cgcgctcatc gaacagcccc
      361 tgacgacgtc gaagggcgag ctggtgacgg ccgtgttcgg gttcacgaac atcgtgttgc
      421 gggcgatcca ggacgcgaag cccgaccggc tgggcgtggc cttcgacctc ggcaagccga
      481 cgttccggca cgagcgatac gcggagtaca aggccacccg ccagcggatg cccgacgaca
      541 tgcgcgagca gatcccgaag gtccgcgagg tcgtgaaggc gctcgggatc ccggtctacg
      601 agcgtgaggg gttcgaggcc gacgacgcaa tcgcaacgct cgtcgggcag gccgaggccg
      661 cggatctcga cgtcacgatc ctgaccggcg acctggacat gctccagctg gtcagcgagc
      721 ggacgaagct gatggtctcg ctgcgcggcg gcatcgcgaa caccgtcgcc tacgacctcg
      781 ccaagatcca ggagcggtgg ggcctccgtc ccgaccagat gctcgactac aaggcgctca
      841 agggcgaccc gaccgacaac atcccgggga tccccggggt gggggagaag acggcctcca
      901 agctgatcgc gacgtggggg acgctcgacg tgctgtacga gcacatcgac gaggtcatgc
      961 cggagaagct gcggccgctg ctcgaggagt accgcgacgt cgtcctcgag agccgcgagc
     1021 tcatgcgcct cgtgcgtgac gtggacgtcg agctggacct gagccgcggg cgggtcgggg
     1081 agtacgaccg cgagacggtc gtccggctgt tccgcgagta cgagttccgg accctgatcg
     1141 accgcctgcc gcccctcatc ggggagcggc cggaggacgc gatcgccgcg atgcgcgagc
     1201 tccgggaggc cgggtttccg gctgcccagg gcgtcgggcg gggcgccggc ggtggccggc
     1261 cgggtggtca agggacggcg cccgtcgacc cgggttcgct gcagctgtcg atggacttcg
     1321 acgtggtctc gggcggcgga ggaggcagcg cgacgggcgg gatcgccgcg acctcgcccg
     1381 ccgtcgaggc gcgggcggag gcgatcgcgg cggcgtcggg cgacgtgccg ggcgccctgg
     1441 ccgcggcgat cgtggacccg ggccggatcg agatcttgga cgcggcaggg gtcgtcggac
     1501 tggagccctg gctgcgcgac caggccgccg tcggggtcgc catcgtggtc gacgacccgc
     1561 ggccgatggc cggcacgctg ctcgcgctgg ccgtcgcggg cgaggacggc cgcgtggtcg
     1621 ccgcggacgg gcccgaggcc tccgacgcgc tgcgccacct cctcgagcgc ctggatgccc
     1681 gtctcgtcgg ccacgaggtc aagccgctgc tgaccgcgcg cttcgccgag gacccgtcgg
     1741 ccgcgcccgt gcccatcgcc ttcgacaccc agatcgccgc ctacctcgtc aacgccgccc
     1801 tccgggccca gaagatcgcc gacgtcgtcg cggagcggct ggacctcgtc ctgccgccgg
     1861 ccgccgccgg cctcccgccg acggccacgg ccgggctcga ggccctgtcc gcggtcgcgg
     1921 tgcggccatc gctcgagcag gcgatgcgcg acgagggcgt ggaccggctg ttcgaggaga
     1981 tcgagctgcc gctgatcccg atcctcgccc ggatggaggc ggccggcatt gcgctggaca
     2041 tggcagccct cgccgaactg gagcgcgagt tcgccgccga gatcgcgcgc ctcgagggcg
     2101 agatctacgc ggccgtcggc caccagttca cgatcggctc gcccaagcag cttggcgaca
     2161 tcctgttcgt cgagctcggg ctcccgaagg gccgcaagac gaagaccggc tactcgaccg
     2221 acgcctcggt gctcgaggag ctgcgcggcg tccatccggt catcgagccc gtgctcgagt
     2281 ggcggatcta caccaagctc cgctcgacct a
//
LOCUS       DBNHXO010000225         1855 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2043401, whole genome shotgun sequence.
ACCESSION   DBNHXO010000225 DBNHXO010000000
VERSION     DBNHXO010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2043401"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..910)
                     /gene="trpA"
                     /locus_tag="AC162A_04735"
     CDS             complement(68..910)
                     /gene="trpA"
                     /locus_tag="AC162A_04735"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR5010180.1"
                     /translation="MSAVATPIHTNDTLGARRVADGFARARAEGRAALIPYVVAGYPD
                     ADASFEIACAAIDAGADLLEVGLPYSDPLADGATLQRASQGALAAGATFDRSLALVAR
                     IAAARPAVPLVPMGYANQLIGGGDGRDRARALSSAGAAAVIVADLTPDEGAPFEHVAA
                     EAGIGVVYLVAPTTDPARRAAVAARSGGFLYCVSLVGVTGARTSLPPTVARLVREVKA
                     VSPVPVAVGFGVSKPAHVRAIAKAGADGVIVASALVDALGPEGRDIAALSRLVKSLRA
                     ATDR"
     gene            complement(907..1275)
                     /locus_tag="AC162A_04740"
     CDS             complement(907..1275)
                     /locus_tag="AC162A_04740"
                     /inference="COORDINATES: protein motif:HMM:NF016152.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HIR5010181.1"
                     /translation="MTIDELRAFCMSLPGTHEKETWGDAEHAGDITFRVKDKIYVMTG
                     QGGGAVSIRTTVEQQADLMAAFPESVSIARYVGRFGWVDVVIDGPGSLDPEVVRDVIH
                     SAWRRTVPKSMLHELEASGS"
     gene            complement(1280..>1855)
                     /locus_tag="AC162A_04745"
     CDS             complement(1280..>1855)
                     /locus_tag="AC162A_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR5010182.1"
                     /translation="AAQLLAVEGRLPDMALACVGGGSNAIGLLSRFIGEPSVRLAVAE
                     AAGDGIATGRHAAAIAGGTPGILHGSRSLMLQDRDGQVVEAHSASAGLDYPGVGPQLA
                     ALAEGGRLEVATATDREAVAAMRTVTRAEGILPALETAHAIAALPKLLAGVEGSGQAW
                     PDEAVVLLGFSGRGDKDLAALEKFADVEPWD"
BASE COUNT          265 a          706 c          654 g          230 t
ORIGIN      
        1 cccgagtatg ccaaggcaga ggtcaggacc tgtcaacgat gctggcgaac ggcgtcgcgc
       61 cccgggctca gcggtcggtg gcggcgcgaa ggctcttcac gaggcgcgac agggccgcga
      121 tgtcgcggcc ttccgggccg agcgcatcga ccagcgccga ggcgacgatc acgccgtccg
      181 ctcccgcctt cgcgatcgcc cggacgtggg cgggcttcga caccccgaag ccgaccgcga
      241 ccggtaccgg cgagaccgct ttgacctcgc ggacgaggcg ggcgacggtc ggtggcagcg
      301 acgtccgggc gccggtcacg ccgaccagcg agacgcagta caggaacccg ccgctgcgag
      361 cagcgaccgc ggcgcggcgc gcggggtccg tcgtcggcgc cacgagatac acgacaccga
      421 tccccgcctc ggcggcaacg tgctcgaacg gggcgccctc gtcgggcgtg aggtcggcga
      481 cgatcacggc ggccgcaccg gccgaggaca gtgcccgggc ccggtcgcgt ccgtcgccgc
      541 ccccgatcag ctggttggca tagcccatgg gcacgagcgg cacggccggg cgggcggcgg
      601 cgatccgcgc gacgagcgcc agcgaacggt cgaacgtcgc gccggccgcg agcgcgccct
      661 gcgacgcgcg ctggagcgtc gccccgtccg cgagcggatc agagtacggc agccccacct
      721 cgagcaggtc cgccccggcg tcgatcgccg cacaggcgat ctcgaagctc gcgtcggcgt
      781 ccgggtagcc cgcgacgacg tacgggatga gggcggcacg accctcggcg cgcgcccgcg
      841 cgaaaccatc ggcaacccgg cgtgcgccta aggtgtcgtt ggtgtggatc ggcgtcgcga
      901 cggcactcac gagccgcttg cctcgagttc gtggagcatc gacttcggaa cggtcctgcg
      961 ccacgccgag tggatgacgt cgcgcacgac ctcgggatca agcgaacctg gcccatcgat
     1021 gacgacgtcg acccagccga accggccgac gtagcgcgcg atcgagaccg actccggaaa
     1081 ggccgccatg agatcggctt gctgctcgac ggttgtccgg atgctgacgg cgccgccgcc
     1141 ctggccggtc atcacgtaga tcttgtcctt gacccggaac gtgatgtcgc ccgcgtgctc
     1201 ggcgtcgccc caggtctcct tctcatgcgt cccgggcagc gacatgcaga aggcgcgcag
     1261 ctcgtcgatc gtcatcgcat cagtcccacg gctcgacatc cgcgaacttc tcgagggccg
     1321 ccaggtcctt gtcgccgcgg ccggaaaatc cgagcagcac cactgcctcg tcgggccacg
     1381 cctgccccga accttcgacc cccgcgagga gctttggcag ggccgcgatc gcgtgtgccg
     1441 tctcgagcgc gggcaggatg ccctcggctc gggtcaccgt gcgcatcgcg gcgacggcct
     1501 cgcggtcggt cgcggtcgcg acctcgagcc gcccgccctc cgcgagcgcg gccagctgcg
     1561 gcccgacgcc cgggtaatcg agtccggccg acgccgagtg cgcctcgacg acctggccgt
     1621 cccggtcctg gagcatcagc gacctcgacc cgtgcaggat cccgggcgtc ccgcccgcga
     1681 tcgccgccgc gtgccgccct gtcgcgatgc cgtcgccggc ggcctcggcc accgccagcc
     1741 gcaccgacgg ttcgccgatg aaccgcgaca gcaggccgat ggcgttcgac ccgccgccga
     1801 cgcaggcgag ggccatgtcc ggcagccgcc cttcgaccgc cagcagctgc gccgc
//
LOCUS       DBNHXO010000226         2744 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3234083, whole genome shotgun sequence.
ACCESSION   DBNHXO010000226 DBNHXO010000000
VERSION     DBNHXO010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3234083"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="AC162A_04750"
     CDS             <1..518
                     /locus_tag="AC162A_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5010183.1"
                     /translation="QLLPEVSMRRLPIAILLVAFALVAGACSSSTGPSWTYAPPTPAP
                     TPGPSGSAAPSGAPSTAPSAGASGEPSAAPSGGGGDAVVITALNLQFTTPEVSAPASV
                     PFVIDFRNEDAGQPHNVEIKDGSGVQKFMGQIVTGVTEAKYDVPALDPGTYQFICTVH
                     VNMKGTLKVGA"
     gene            532..1281
                     /locus_tag="AC162A_04755"
     CDS             532..1281
                     /locus_tag="AC162A_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0002196 - Ser-tRNA(Ala) deacylase activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HIR5010184.1"
                     /translation="MTPRVDEVCYRDAYARTLGARILEVAEGDGAPLVVLDTTVFYPG
                     GGGQPSDRGVILRTDDGRSWTVRAARRADGEIVHELEPPADGDLPRAGDAVSVDLDWA
                     RRHALMRTHTALHALCGVVRRDYGALVTGGNMEPGSGRMDFEFERMSGDLVEEIEAKV
                     NVELHAGRDVRVNVLPRDEAFAIPDLIRTKVNLLPEGIAEIRTIEIVGLDLQADGGTH
                     VANTGEVGGIRVTGYESKGRINKRIRIELTD"
     gene            complement(1278..2141)
                     /locus_tag="AC162A_04760"
     CDS             complement(1278..2141)
                     /locus_tag="AC162A_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010185.1"
                     /translation="MRTFRDPDDAIRAWLEEGPLVFPDQNERAIDVATQSMSQRRPAM
                     RLPWRFPIMTTLPKVVAVGMAVVAVTLGGAMLLGQRSASPGVDANPTPSPTTAPSPSP
                     SARASASLLDPTTWAPFTSERYGYSAAYPPDFGTIPSTKFWVIPESDSTSSAEWDMFG
                     RNGTYDPTWSATSIRLPDGVTEDAWADAYRLTQDDPGRWGAACYPSTAPLSPVTIDGR
                     TGSLRVGCGDIEALVVADGHMYSFGGWTTTTTTPGVPDEFRALFEAWLTTIKLDPASA
                     LEPPAASPSPR"
     gene            complement(2138..2692)
                     /locus_tag="AC162A_04765"
     CDS             complement(2138..2692)
                     /locus_tag="AC162A_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5010186.1"
                     /translation="MRAGLVERAREGDHDAFTELVAVDSDRCYAIAYRILRDVERAQD
                     AVQQAFMLVWRELPRLRDPARYEVWLHRLLVNACYEESNRHRRWSVHIRALPEDGPGG
                     RDPEIVAVDERDAMERAFRRLSPEHRAVIVLHHHVGLPLGTIGEIVGVPVGTVKSRLH
                     YATHSLRAALAADDAPDLREVRPA"
BASE COUNT          401 a          952 c          987 g          404 t
ORIGIN      
        1 cgcagttgtt gcccgaggtc tcgatgcgcc gcctcccgat cgccatcctg ctggtcgcct
       61 tcgcgctggt ggcaggcgcc tgctcgtcgt cgaccggtcc cagctggacc tatgctccgc
      121 cgacccccgc cccgacgccc ggtccatccg gctcggccgc accgtcggga gcgccgtcga
      181 cggccccctc tgcgggcgcc tctggcgaac cgagcgcggc ccccagcggc ggtggcgggg
      241 acgcggtcgt gatcaccgcg ctgaatctgc agttcaccac gcccgaggtc agcgcccccg
      301 cgagcgtccc gttcgtgatc gacttccgca acgaggacgc gggccagccg cacaacgtcg
      361 agatcaagga cggcagcggt gtccagaagt tcatgggcca gatcgtgacc ggcgtgaccg
      421 aggccaagta cgacgtcccg gcgctcgacc ccggcacgta ccagttcatc tgcaccgtgc
      481 acgtgaacat gaaggggacg ctcaaggtcg gcgcttgatc ccggcccggc catgacccct
      541 cgcgtcgacg aggtctgcta ccgcgacgcg tacgcgcgga cgctcggggc gcgcatcctg
      601 gaggtcgccg agggcgacgg cgccccgctg gtcgtgctcg acacgaccgt gttctacccg
      661 ggcggtggcg gccagccgtc ggatcgcggc gtgatcctgc gcacggatga tggacgctcc
      721 tggacggtcc gcgcggcccg ccgggccgac ggcgagatcg tgcacgagct cgagccgccc
      781 gccgacggcg acctgccccg ggccggcgat gcggtgtcgg tcgacctcga ctgggcgcgc
      841 cgtcacgcgc tgatgcgcac ccacacggcg ctccacgccc tgtgtggcgt cgtgaggcgg
      901 gattacggcg cgctcgtcac gggcggcaac atggagcccg gctccgggcg catggacttc
      961 gagttcgagc ggatgtcggg cgacctggtc gaggagatcg aggccaaggt caacgtcgag
     1021 ctgcacgcgg gccgcgacgt gcgcgtgaac gtgctgccgc gcgacgaggc gttcgcgatc
     1081 ccggacctga tccggaccaa ggtcaacctg ttgcccgagg gcatcgccga gatccggacg
     1141 atcgagatcg tcgggctcga cctccaggcc gatggcggca cgcacgtggc caacaccggc
     1201 gaggtcggcg gcatccgggt caccggctac gagtcgaagg gccggatcaa caagcggatc
     1261 cggatcgagc tgaccgacta gcgcggcgac gggctggcgg ccgggggctc gagcgccgaa
     1321 gcgggatcga gcttgatcgt cgtgagccag gcctcgaaca gcgcgcgaaa ctcgtcgggg
     1381 acaccgggtg tcgtggtcgt cgtcgtccat cccccgaagc tgtacatatg cccatcggcg
     1441 acgacgagcg cctcgatgtc tccgcacccg acgcgcaggc tgcctgtgcg gccgtcgatg
     1501 gtcactgggc tcagcggtgc ggtcgacggg tagcacgccg cgccccatcg acctgggtcg
     1561 tcctgggtaa gtcgatacgc atcggcccag gcgtcctcgg tcaccccatc gggcaggcgg
     1621 atcgaggtgg cggaccacgt cgggtcgtac gtcccgttcc tgccgaacat gtcccactcc
     1681 gccgagctcg tcgagtcgct ctccgggatc acccagaact tcgtcgacgg aatggtgccg
     1741 aagtcgggcg ggtacgccgc gctgtagccg tagcgctccg aggtgaacgg cgcccacgtt
     1801 gtcgggtcga ggagcgacgc cgacgcccgg gcggacgggg acggcgacgg cgccgtggtc
     1861 ggcgacggcg tcggatttgc atccacgccg ggcgacgccg agcgctgtcc gaggagcatg
     1921 gcgccgccga gcgtgacggc gacgaccgcc atcccgacgg cgacaacctt gggaagcgtg
     1981 gtcatgatcg ggaacctcca cggcaggcgc atggccggcc gccgttggga catcgattgg
     2041 gtggcgacgt cgatcgcgcg ctcgttctgg tcggggaaga cgagcgggcc ttcctccagc
     2101 caggcgcgga tcgcgtcgtc gggatctcgg aaggtcctca tgccgggcga acctcccgca
     2161 ggtcaggtgc gtcgtccgcg gcgagtgccg cgcgaaggct gtgggtcgcg tagtggagcc
     2221 gggacttcac ggttcccacc gggaccccga cgatctcgcc gatggtcccg aggggcagcc
     2281 cgacgtggtg gtgcaggacg atgaccgccc ggtgctcggg agacaggcgt cggaacgccc
     2341 gctccatggc atcgcgttcg tcgaccgcca cgatctctgg atccctgccg cccggaccgt
     2401 cctcggggag agcccggatg tggacgctcc agcggcggtg gcggttcgac tcctcgtagc
     2461 atgcgttcac gagcagccgg tgcagccaca cctcgtagcg ggcggggtcg cgcagtcgtg
     2521 ggagctcgcg ccacacgagc atgaatgcct gctggacggc gtcctgggct cgttcgacat
     2581 cgcgcaggat ccgatacgcg atggcgtagc agcgatcgct gtcgacggcg accagctccg
     2641 tgaaggcatc gtgatcgcct tcccgcgccc gttccaccag tccagcccgc atggcggacc
     2701 tccctggggc gcctggtctg ggcgctcaac cgtatacaga tggt
//
LOCUS       DBNHXO010000227         2520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5417981, whole genome shotgun sequence.
ACCESSION   DBNHXO010000227 DBNHXO010000000
VERSION     DBNHXO010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5417981"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="AC162A_04770"
     CDS             complement(<1..612)
                     /locus_tag="AC162A_04770"
                     /inference="COORDINATES: protein motif:HMM:NF013871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010187.1"
                     /translation="MVAVLDTLRAHGHAAYLVGGSLRDALLDREPADWDLATDALPPA
                     IAALFPGAVYENRFGTVAVRRDHEVFEITTFRVEHDYADHRRPHRVEFGGDIVGDLSR
                     RDFTVNAIAWGGPADEAGDGDRGRPNTVVDPFDGLGDLAARRLRAVGDPAARFREDAL
                     RMVRAVRLAATLGMTVEPATLDAIAGQASLAEHLSGERVGAELS"
     gene            complement(542..1195)
                     /locus_tag="AC162A_04775"
     CDS             complement(542..1195)
                     /locus_tag="AC162A_04775"
                     /inference="COORDINATES: protein motif:HMM:NF025637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR5010188.1"
                     /translation="MNGVVLVLNQNYEPLNVCNIPRAFRLVFGSKAEVIEYDHAEIRT
                     VRTVYRAPSVIRLQHQIRRPRPRVKLTRREIFSRDRHTCQYCGRQANDLTLDHVLPRH
                     RGGGHTWENLVAACKPCNHRKGGRTPEEAHVRLVRVPFEPRSDIYSLFKPYLEDVRNE
                     AWRTYLFLGRSRAAGGRPGRRRGCGPPRFRRPGSRGGGPRHAAGARARGVPRRWLAA"
     gene            complement(1292..1507)
                     /locus_tag="AC162A_04780"
     CDS             complement(1292..1507)
                     /locus_tag="AC162A_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010189.1"
                     /translation="MHEGSATDTRRSRSKTAAATREALLVQHAEARRLRNAAELGGHE
                     WEQASAEVGRIEVEIARIERAMDPPRV"
     gene            complement(1559..>2520)
                     /locus_tag="AC162A_04785"
     CDS             complement(1559..>2520)
                     /locus_tag="AC162A_04785"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR5010190.1"
                     /translation="VDAVVVAQAFHWFDAIRALSEIHRVLRPGGRVLLAMNQRDESVP
                     WVRALGDLIRTLAVGEPQVWDDAWRASLRRCALFEAWSTVAFRQAQLLTREGVLDRVA
                     SVSFVAAAEPSAQAEVLANVEALLRDDPDTAGRETIELPYDTTLMWATRRTIEPGLDG
                     VVASVNLNRGGVPKPPVDGTVVRTLGLDGDGHANPDVHGGPTAAVCLYPQEAIERVRA
                     DGHQAFPGSYGENLTLLGIDWAALREGDRLDLGDEGGGEPGPLLELTQYAAPCATQSH
                     WFLEGRIARISHKVRPDDARWYARVLREGPVAPGMSVRVVREG"
BASE COUNT          336 a          906 c          930 g          348 t
ORIGIN      
        1 cgagagctcg gcgccgaccc gctcgccgga gaggtgctcg gcgagcgatg cctggcccgc
       61 gatggcgtcg agcgtcgccg gttcgacggt catcccgagc gtggccgcga gtcgcacggc
      121 ccgcaccatc cgcagggcgt cctcgcggaa ccgtgcggcg ggatcgccga ccgcccgcag
      181 ccgccgtgcc gcgaggtcgc cgaggccgtc gaacgggtcg accacggtgt tcgggcgtcc
      241 acggtcgccg tcgccggcct cgtccgcggg cccaccccac gcgatcgcat tgaccgtgaa
      301 gtcacggcgc gacaggtccc ccacgatatc gccgccgaac tcgacccggt gtggccgccg
      361 gtggtctgcg tagtcgtgct cgacgcggaa cgtggtgatc tcgaacactt cgtggtcgcg
      421 ccgcacggcg accgtcccga accggttctc atacaccgcg ccggggaaca gggcggcgat
      481 cgcgggcggg agggcgtcgg tcgcgagatc ccagtccgcg ggttcccggt ccaggagggc
      541 gtcacgcagc gagccaccga cgaggtacgc cgcgtgcccg tgcgcccgca gcgtgtcgag
      601 gaccgccacc acggctgccg gggcggcgga agcgaggcgg tccgcagcct cggcgacgtc
      661 cgggcctgcc accggcggct cggctccggc cgaggaagag gtacgtcctc caggcttcgt
      721 tccgaacgtc ttcgagatac ggcttgaaca gcgagtagat gtcgctgcgc ggctcgaacg
      781 gcacccgtac caggcgcacg tgcgcttcct ccggcgtccg cccacccttg cggtggttgc
      841 acggcttgca ggccgcgacg aggttctccc acgtgtggcc gccgccgcgg tggcggggca
      901 ggacgtggtc gagcgtcagg tcgttcgcct ggcgcccgca gtactggcag gtgtgccggt
      961 cgcggctgaa gatctcgcgc cgggtcagct tcacccgcgg ccgggggcgg cggatctggt
     1021 gctggagccg gatcaccgac ggcgcccgat acaccgtccg gacggtccgg atctcggcgt
     1081 ggtcgtactc gatcacctcg gccttggagc cgaacaccag ccggaacgcc cgcgggatgt
     1141 tgcagacgtt caggggctcg taattctggt tcagcacgag cacgaccccg ttcatcggcc
     1201 gaacgatagc agcacggctg gaggcgtcag attcgggggt cctgggggtc gcgaacgggg
     1261 tcgcggcgag ggtgctggcg gccgtctccc ctcagaccct gggcgggtcc atcgcccgtt
     1321 cgatccgggc gatctcgacc tcgatccggc cgacctcggc agacgcctgc tcccactcgt
     1381 ggccgccgag ctccgcggca ttgcgcagcc ggcgggcctc ggcgtgctgg accagaagcg
     1441 cctcgcgggt ggccgcggcg gtcttgctcc tgctccggcg ggtgtcggtc gcggatcctt
     1501 cgtgcatgtc gtgcgcagcg tagcgcgccg ggaggtcgag cgcgcgcacg cgcgagcctc
     1561 agccctcgcg gaccacgcgc acggacatcc caggggcgac cggtccctca cgcagcacgc
     1621 gggcatacca gcgggcgtcg tcggggcgga ccttgtggct gatgcgggcg atccggccct
     1681 cgaggaacca gtgcgattgg gtcgcgcacg gcgccgcgta ctgggtcagc tcgaggagcg
     1741 ggccgggctc cccgccgccc tcgtcgccga ggtcgagccg gtcgccctcg cggagcgccg
     1801 cccagtcgat cccgagcaac gtcaggttct caccgtacga gcccgggaac gcctggtggc
     1861 catctgcgcg gacccgctcg atcgcctcct gcgggtagag gcagacggca gcggtcggac
     1921 cgccgtggac gtcggggttc gcgtgtccgt cgccatcgag gccgagcgtc cggacgaccg
     1981 tgccgtcgac gggcggcttc ggtaccccgc cccggttcag gttgacggag gcgacgacgc
     2041 cgtcgaggcc aggttcgatg gtccggcggg tcgcccacat cagcgtggtg tcgtagggca
     2101 gctcgatggt ctcccggccg gccgtgtcgg ggtcgtcacg aagcagggct tcgacgttcg
     2161 ccaggacctc cgcctgcgca gacggctcgg cggcggccac gaagctcacg gacgcgaccc
     2221 ggtcgagcac gccctcgcgc gtgagcagct gggcctggcg gaacgcgacg gtcgaccatg
     2281 cctcgaacag cgcgcagcgc cggagcgacg cccgccaggc gtcgtcccag acctgcggct
     2341 cgccgacggc gagcgtccgg atcaggtcgc cgagcgcacg aacccacggc accgactcgt
     2401 cccgctggtt catcgccagc aggacacggc cgccgggtcg cagcacccgg tggatctcgg
     2461 acagggcgcg gatcgcgtcg aaccagtgaa acgcctgggc gacgaccacc gcgtcgaccg
//
LOCUS       DBNHXO010000228         2310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3036757, whole genome shotgun sequence.
ACCESSION   DBNHXO010000228 DBNHXO010000000
VERSION     DBNHXO010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3036757"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="AC162A_04790"
     CDS             complement(<1..197)
                     /locus_tag="AC162A_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010191.1"
                     /translation="MQKPPAGAQRHADRLHPARAHATTASRVNRLSIRVVRSAQSGFH
                     GKGAALTTLATRFARAARPLTT"
     gene            complement(420..1799)
                     /gene="rho"
                     /locus_tag="AC162A_04795"
     CDS             complement(420..1799)
                     /gene="rho"
                     /locus_tag="AC162A_04795"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR5010192.1"
                     /translation="MPNGSDQRPTRNRRPRRRSQTSRLPVTDQDEVAELEAARERNDL
                     DIRDLREMSVKELIEVGARLEIDGEPTERRDDLVDQILQAQTERAGLDYAFGILDIVD
                     EGYGFMRRHGLLPSPDDVYVSSSQVRRFGLRVGDRVTGAVRAPHEGEKYWGLIRVDSV
                     NSADTDTARRRPYFNDLTPIHPIELINLESDPKNLSQRLVNLVNPLGKGQRALIVSPP
                     KAGKTMLLKSIANGISANYPDILLMVALIGERPEEVTDMRRSVHGEVISSTFDEPTEN
                     HTHVAEMALEIAKRQVETGRDVVILLDSITRLARAYNLALPPSGRTLSGGIDPIALYP
                     PKRFFGAARKIEEGGSLTIIATCLVDTGSRMDDVIYEEFKGTGNSELILDRKLAEKRI
                     FPAIDVQRSGTRREELLLEEGTLKMVWTMRRMLSAVGTNEGIELLLNRIAKTETNAQF
                     LGTLTKTLD"
BASE COUNT          328 a          728 c          868 g          386 t
ORIGIN      
        1 gtggtcaatg ggcgcgccgc gcgcgcgaac ctcgtcgcca gcgtcgtgag ggcggcgccc
       61 ttgccgtgga aaccggattg cgcggaacga accacgcgga tgctcaaccg gttgacccgg
      121 gaggccgtcg tcgcgtgggc gcgtgctggg tgcaggcggt cggcatggcg ctgagcgccg
      181 gccggtggct tctgcaatac ggtctcctgg cacgtcgatc ggccggtgga caggacacca
      241 cgtggggcgt cgggccgatc atttccgggg tcgcgattgg gtgcggacgt ccccccgcgg
      301 ccgatcccgg aagcccgggt caacccgtaa agttctgccg aaccgtacca gaaccgttac
      361 ggggcacgca aacgcgcggc gtcacaacgg ccccgaatct gggtgaatcg tggaccccgc
      421 tagtcgaggg tcttggtcag cgtcccgagg aactgggcgt tggtctcggt cttggcgatc
      481 cggttgagca ggagctcgat gccctcgttg gtgccgaccg cggacagcat ccggcgcatc
      541 gtccagacca tcttgagcgt gccctcctcg aggagcagct cctcacgccg ggtgccggac
      601 cgctggacgt cgatggccgg gaagatgcgc ttctcggcga gcttccggtc gaggatgagc
      661 tcagagttgc cggtgccctt gaactcctcg tagatgacgt cgtccatgcg cgagccggtg
      721 tcgacgaggc aggtcgcgat gatcgtgagc gagcccccct cttcgatctt ccgcgcagcc
      781 ccgaagaacc gcttgggcgg atagagcgcg atggggtcga tgccgcccga cagggtccgg
      841 ccgctgggcg gcagggccag gttgtaggcg cgcgcgaggc gcgtgatcga gtcgagcagg
      901 atcacgacgt cgcggccggt ctcgacctgg cgcttggcga tctcgagggc catctcggcg
      961 acgtgggtgt ggttctcggt cggctcgtcg aaggtcgagc tgatgacctc gccgtggacc
     1021 gagcgccgca tgtccgtgac ttcctcgggc cgctccccga tgagggcgac catcagcagg
     1081 atgtccgggt agttggcgct gatcccgttc gcgatgctct tgagcagcat cgtcttgccg
     1141 gccttgggcg ggctgacgat cagcgcacgc tggcccttgc cgagcgggtt gacgaggttg
     1201 accaggcgct gactgaggtt cttcgggtcg gactcgaggt tgatcagctc gatcgggtgg
     1261 atcggcgtca ggtcgttgaa gtaggggcgg cggcgcgcgg tgtccgtgtc ggcgctgttg
     1321 acgctgtcga cgcggatcag cccccagtac ttctcgccct cgtgcggggc gcggacggca
     1381 ccggtgaccc ggtcgccgac gcggaggccg aaccggcgca cctggctcga cgagacgtac
     1441 acgtcgtcgg ggctcggcag caggccgtgc cggcgcatga acccgtagcc ctcgtcgacg
     1501 atgtcgagga tgccgaacgc gtagtcgagg ccggcgcgct cggtctgggc ctggaggatc
     1561 tggtcgacca ggtcgtcgcg gcgctcggtg ggttcgccgt cgatctcgag gcgcgcgccg
     1621 acttcgatca gctccttgac ggacatctcg cgcaggtcgc ggatgtccag gtcgttacgc
     1681 tcgcgcgcgg cctcgagctc ggcgacctcg tcctgatcgg tgacgggcag gcggctggtc
     1741 tgggatcgac gacgcggcct tcgattgcgg gtcgggcgct ggtcggaacc gttcggcatg
     1801 gggttgggca cactccggga ggtggagtcc gacaccggca agggaccggc ggggaacgat
     1861 gaagtcgcgc cacgggccgt gattcgcgcg gaatcggatc gccgtcgcgc gcggccggtg
     1921 gggacgcatg gagcatatcg cgcgccgcgt cacggcgtca acgttcgtgc ggtcgcgtag
     1981 gcgctgatcg ccccgcgtgc agtccagcgg tcggcggcca gatcctgcac gccaggcgag
     2041 cgaaccaccg ggaatcgccg ccgtaaccgg caacctgctc gccgggcgat caatccggcg
     2101 gatgaaccgc tgcagtgaac gctgggcgtg cagcgcgggc aagcgacctg taggcgtgct
     2161 acgccttgac ggccatcgcg gcgcgagcgg cctcccagtc ggccttgaac cgggtgatcc
     2221 cgagatccgt cagcgggtgg tggaccatct gctggaggat cttgaacggg agcgtcgcga
     2281 tgtgcgcgcc ggccagcgcc gagtcggtga
//
LOCUS       DBNHXO010000229         5860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_458552, whole genome shotgun sequence.
ACCESSION   DBNHXO010000229 DBNHXO010000000
VERSION     DBNHXO010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_458552"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..752
                     /locus_tag="AC162A_04800"
     CDS             45..752
                     /locus_tag="AC162A_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010193.1"
                     /translation="MRRSTRRVVGTILFLIALACAIPAAVAATVTGGCQVQGTSTSGG
                     TIDLTTAKEWHLKKDDIAGGSGTSPVDQTAASVGAYALGLQLPVASGSGNGGKSGAVQ
                     GVSVAAYSALGQRFVVAGKSDSCSGQVVIIIDDVNPAMTALGGGGIGALVLGALVLLG
                     TMFLGGGILSRIVGLVFGALAGLGLALALEQFGILDPTTFIGLVIAIVGAVLGLILPG
                     TFHRGASNDAGAEDAPA"
     gene            885..1736
                     /locus_tag="AC162A_04805"
     CDS             885..1736
                     /locus_tag="AC162A_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR5010194.1"
                     /translation="MPRNVRGALLQATWTGDKESMIQKHEGYAHEAAKQGAQVMLFQE
                     LFYGPYFCQVQDRSYYSYTELIPDGPTTKRMQDLAKQTGMVLVVPMYEEDTTASGIYY
                     NTAAVIDADGKYLGKYRKTHIPHVQGFWEKFYFRPGNMGYPVFETAVGKVGVYICYDR
                     HFPEGARALGLNGAEMVFIPSATSRGLSEYLWRVEQVSHALANGYFVGTINRVGIESD
                     IGPDDFYGQSYFVDPRGQFVGAVGSPHDEELLIRDMDLDLITEVRQTWQFYRDRRPDQ
                     YGDLVAP"
     gene            1920..2930
                     /locus_tag="AC162A_04810"
     CDS             1920..2930
                     /locus_tag="AC162A_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017207121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HIR5010195.1"
                     /translation="MQFGFTLKPDHTIERTLALTKQAEAAGFEHGWLFDSHVLWRDPY
                     PLLTLMAGVTTSLRLGTCVTNPATREPSVTASTLALLDEISGGRMDLGIGRGDSARRV
                     LGKPPTTMATLEEAIRVIRALVAGETIAYEGTDLHFPWTQGWTLPVWVAGYGPMALAM
                     TGRVADGVILQLADPDLIAWFVGQLRDAEVAAGRPRGSVRVQAAAPAHVGDLADCRER
                     TRWFPALVSNHVVDLVNKYPRDQLPESLTGYVQDREGYDYLHHAEVGSSNAGFVGDEV
                     TDRFCVLGSVEDHIAKLGRLAKAGVDQFNIYLMNGDEERILEIYGAEIIPATRAFEAA
                     GT"
     gene            complement(2941..4572)
                     /locus_tag="AC162A_04815"
     CDS             complement(2941..4572)
                     /locus_tag="AC162A_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013005556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5010196.1"
                     /translation="MNKRLPLVALAGAQFIMVLDQSVMNVSISQLVDEFDTTVSVIQA
                     VITLYCLVMAMLMLTGGKIGDIIGRRRAFVIGLVIYACGSGLTAVSQTVAQLTLGWSI
                     LEGIGAALVLPAMAALIAGNFEGKERKAAYAVIGGVAGAGIAIGPILGGWATTELSWR
                     VVFVGEVVIVLGILAMVRYVQDAPRTGPKPRLDVVGSVLSAAGLGAVVLAVLQSTTWG
                     LIQPKDSPIEPFGLSLTPFVLAVGGVLLWSFVTWQRRREATGNDPLVHLDLIKIPPLR
                     SGLVGLFSQNLILMGVFFTIPLYLQLVLGLDALQTGIKMLPASIAMFFAAAAGSRLSG
                     RYAVRAITRAGLATIFVSCLALLATIRPDLADLAFAVSMGLLGVGMGLVASQLGLVVQ
                     SSVDASGRGEAGGLQYTGQQLGSSLGVALIGAIVFAGLASTFVGTIQGDPRISAEVAA
                     QVGVAAEAGVDFVSSEQIAVAATEAGLDEATTVAIVEDYEQAQLQSLKAGLLAAALIA
                     LLSLASTRDLPSSKPSEAVESDEPLDPGGAEPAAA"
     gene            4839..5042
                     /locus_tag="AC162A_04820"
     CDS             4839..5042
                     /locus_tag="AC162A_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010197.1"
                     /translation="MATFALHRRRDPFWEVDGPAARAKRRKHRFVSTIAFAAAMAAVV
                     GAAYMWVTQVGLVGFVQAQLGIG"
     gene            5328..>5860
                     /locus_tag="AC162A_04825"
     CDS             5328..>5860
                     /locus_tag="AC162A_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008744819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5010198.1"
                     /translation="MRTLIANGTIVTADGSYDADVLVDGETIAQIGSGLAASGVTADE
                     TIDAVGRYVIPGAIDVHTHMELPFGGTFAKDTFETGTRAAAFGGTTCNVDFAVQSRGQ
                     SLRAGLDAWHAKAEGNAVVDYGFHMIMSDVNDDTLAEMDQLVSEGVPDFKLFTAYPGV
                     FYSDDAAIFRAMQQTGR"
BASE COUNT          932 a         2052 c         2035 g          841 t
ORIGIN      
        1 cgccgccccg ccgcggcggg gaccgcgaaa cgcgagggat gcccatgcgc cggtctacgc
       61 gtcgggtcgt cggcacgatc ctgttcctca tcgcgctcgc ctgcgcgatc ccggcggcgg
      121 tcgccgccac cgtgaccgga ggctgccagg tccagggcac ctcgacctcc ggcggcacga
      181 tcgacctcac caccgcgaag gaatggcacc tcaagaagga cgacatcgcc ggcggcagcg
      241 ggacctcgcc cgtcgaccag acggccgcgt cggtcggcgc ctacgcgctc gggctgcagc
      301 tcccggtcgc gtcgggcagc ggcaacggcg gcaagtcggg tgcggtccag ggcgtgtcgg
      361 tcgccgcgta ctcggcgctt ggtcagcggt tcgtcgtcgc gggcaagtcc gactcgtgca
      421 gcgggcaggt cgtgatcatc atcgacgacg tgaatcccgc catgacggcg ctcggtggcg
      481 gtggcatcgg cgccctggtg ctcggcgcgc tcgtgctcct gggcacgatg ttcctgggcg
      541 gcggaatcct gtcgcggatc gtcgggctcg tgttcggcgc cctcgccggg ctgggcctcg
      601 cgctggcgct cgagcagttc gggatcctcg acccgacgac cttcatcggg ctcgtcatcg
      661 ccatcgtcgg cgcggtcctc ggtctgatcc tgcccggaac gttccatagg ggcgcgtcga
      721 acgacgcggg cgccgaggac gctcccgcct gacgcccgcc gcccgggacc cgtggccggc
      781 cgcgggtccc gcggcgtatc cttccgactc tcgccggccc gcgccggccg atcgatcatc
      841 ccgcgctcgg atccacgccg cgcgcacctt cgaggagggc ttcgatgcct cgaaacgtcc
      901 gaggcgcgct gctgcaggcg acctggaccg gcgacaagga atcgatgatc cagaagcacg
      961 agggttacgc ccacgaggcc gccaagcagg gcgcgcaggt gatgctgttc caggagctgt
     1021 tctacggccc gtacttctgc caggtccagg accgctcgta ctacagctac accgagctca
     1081 tcccggacgg gccgacgacg aagcggatgc aggacctcgc gaagcagacc gggatggtcc
     1141 tggtggtgcc gatgtacgag gaggacacga cggcgtcggg catctactac aacaccgccg
     1201 ccgtgatcga cgccgacggc aagtacctgg gcaagtaccg caagacgcac atcccgcacg
     1261 tccaggggtt ctgggagaag ttctacttcc gccccggcaa catgggctac ccggtgttcg
     1321 agacggccgt cggcaaggtc ggcgtctaca tctgctacga ccgccacttc ccggagggcg
     1381 cccgggcgct cggcctcaac ggcgccgaga tggtgttcat cccgagcgcg acgagccgcg
     1441 gcctgtccga gtacctgtgg cgggtcgagc aggtgagcca cgcgctcgcc aacggctact
     1501 tcgtcggcac gatcaaccgc gtgggcatcg agtccgacat cggccccgac gacttctacg
     1561 gccagagcta cttcgtggac ccgcgaggcc agttcgtcgg tgccgtgggc agcccgcacg
     1621 acgaggagct gctgatccgc gacatggacc tcgacctgat caccgaggtt cgccagacct
     1681 ggcagttcta tcgcgaccgc cggcccgacc agtacggcga cctcgtcgcc ccgtaacgcc
     1741 gctgtcgtcc gcgacgacat cagtctgcgg acgcgctatc atcgcgcggc tcggctgcct
     1801 ggaggagggc agccgagccc tttcatgtcc ggggctcgca tgtgccggag cgttccgacg
     1861 ggcacgaggg tcggtgtacc ctgtcgcctc caccgacgtc ccggaggcgg ttcgaggcga
     1921 tgcagttcgg gttcacgctc aagcctgatc acacgatcga gcgcacgctc gcgctgacga
     1981 agcaggccga ggcggccggc ttcgaacatg gctggctgtt cgacagccac gtgctgtggc
     2041 gcgatccgta tccgctcctc accctgatgg ccggggtgac cacgagcctg cgcctcggga
     2101 catgcgtgac caatcccgcc acgcgtgagc cgtcggtaac tgcgtccacc cttgccctgc
     2161 tggacgagat ctccggtggc cggatggacc tcggcatcgg gcgcggcgac tcggcccggc
     2221 gcgtgctggg caagccgccg acgacgatgg cgacgctcga agaggcgatc cgggtcatcc
     2281 gcgcgctcgt ggcgggcgag acgatcgcgt acgagggaac ggacctccac ttcccgtgga
     2341 cgcagggctg gacactcccg gtctgggtcg ccggctacgg gccgatggca ctggcgatga
     2401 ccggtagggt cgccgacggc gtgatcctcc agctcgccga cccggacctg atcgcgtggt
     2461 tcgtcgggca gctccgcgac gccgaggtcg ccgcgggccg gccgcggggt tcggtgcggg
     2521 tccaggccgc cgcgccggct cacgtcggcg acctggccga ctgccgcgag cggacgcgct
     2581 ggttcccggc cctcgtgagc aaccacgtcg tcgacctcgt caacaagtac ccgcgcgacc
     2641 agctgcccga gtccctcacc ggctacgtgc aggaccgcga gggctacgac tacctgcacc
     2701 acgccgaggt cggctcgtcg aacgccggat tcgtgggcga cgaggtcaca gaccggttct
     2761 gcgtcctcgg gtccgtggag gaccacatcg ccaagctcgg gcgcctcgcg aaggccggcg
     2821 tcgaccagtt caacatctac ctgatgaacg gcgacgagga gcgcatcctc gagatctacg
     2881 gcgcggagat catccccgcg acacgcgcct tcgaggcagc agggacctga tcgccggccg
     2941 ctaggcggcc gccggctccg ctcctccagg atcgagcggt tcgtccgact cgacggcttc
     3001 cgacggcttc gatgacggca ggtcgcgagt ggaggcgagg gacaagagcg cgatcagcgc
     3061 cgccgcgagg agtcctgctt tcagcgactg gagctgtgcc tgctcgtagt cctcgacgat
     3121 cgccacggtc gtcgcctcat cgagacctgc ctccgtggct gcgacggcga tctgctccga
     3181 agagacgaag tccaccccgg cttcggcggc tacgccgacc tgcgccgcaa cctccgcgct
     3241 gatgcgtggg tcgccctgga tcgtccccac gaaggtgctg gccaggcccg caaacacgat
     3301 cgccccgatg agggcgacgc cgagcgagga accgagctgc tgtccggtgt actgcaggcc
     3361 ccctgcctca ccacgtcccg acgcgtcgac ggaggattgg acgaccaggc cgagctgcga
     3421 ggccaccagg cccatgccca ccccgagcag ccccatcgac accgcgaacg ccaggtcggc
     3481 caggtcgggc ctgatcgtgg cgaggagggc gaggcacgac acgaagatgg tcgcgagacc
     3541 ggcccgcgtg atcgcccgga ccgcgtatcg cccggacagg cgcgagcctg cggccgccgc
     3601 gaagaacatg gcgatggagg ccggcagcat cttgatgccc gtctggagcg cgtcgaggcc
     3661 gagcacgagc tgcaggtaca gcgggatcgt gaagaacacg cccatcagga tgaggttctg
     3721 cgagaacagc cccacgagcc cggaccggag cggcgggatc ttgatcaggt cgaggtggac
     3781 cagcgggtcg ttgcccgtgg cctcgcgcct tcgctgccag gtcacgaagg accacagcag
     3841 caccccgccg actgcgagga caaacggggt cagtgagagc ccgaacggct cgatcggcga
     3901 gtccttgggc tggatcagcc cccacgtcgt ggactggagg accgcgagga ccaccgcccc
     3961 gaggccggcc gcggagagca ccgagccgac gacgtcgagc ctgggcttcg ggcccgtgcg
     4021 cggcgcgtcc tggacgtagc ggaccatcgc gaggatgccg aggacgatga ccacttcacc
     4081 gacgaacacg acccgccacg acagctcggt ggtcgcccac ccgccgagga tcgggccgat
     4141 ggcgatcccg gcgcccgcga caccgccgat cacggcgtag gcagccttgc gctctttgcc
     4201 ctcgaagttg ccggcgatca acgccgccat cgccggcagc acgagggcgg cgccgatgcc
     4261 ctcgaggatc gaccagccga gggtcagctg tgcgaccgtc tgcgacacgg cggtcaggcc
     4321 cgacccgcac gcgtagatga ccaggccgat cacgaacgcg cggcgacgac cgatgatgtc
     4381 gccgatcttg ccgccggtga gcatcagcat cgccatgacg aggcagtaga gcgtgatgac
     4441 ggcctggatc accgagaccg tcgtgtcgaa ctcgtccacc agctggctga tcgagacgtt
     4501 catgaccgac tggtcgagga ccatgatgaa ctgggcgccg gcgagcgcga cgagcggcaa
     4561 cctcttgttc atcgggtctc ctcttccgcc cacggttcgg atgctgtcga gggtaccggg
     4621 tggcgcgggc gccatcgtgg acatcctgaa ccatgggcgt gaccggcggt gtggtgcgtg
     4681 cgcgcacctc cggagatcga cctattccac ggcctgcgcg aatcggcgag gtgcgctccg
     4741 ggggcgtccg gtgcagatcc gacgggggtc cgggggccac aggtcactct cgatcttgcg
     4801 gcccggaacc ccgacactgg tcccattgga ggtgcccgat ggcaacgttc gccctgcatc
     4861 gtcgtcgtga tccgttctgg gaggtcgatg ggcccgccgc ccgggcgaag cggcggaagc
     4921 accggttcgt ctcgacgatc gcgttcgctg ccgccatggc cgccgtcgtc ggcgccgcgt
     4981 acatgtgggt cacccaggtc ggcctcgtcg ggttcgtcca ggcgcagctc ggcatcggct
     5041 gacaggtcag gtggcgccgc gcgctagccc ctgaggccag cgaatccgcc ccaaaggcca
     5101 ccaacgacgg catctggcct cggttgcggg tcccgcagac acgttcgaag cgcgaaggac
     5161 gacgatcctg gcgcacggcg gtcgttcgag gcgaatcgct ggcccgccag gccagacccg
     5221 ggcttggcgt cgacctgttg gcgatgggat ggatcgcccg gacccccgcg ccggggacgc
     5281 cgcatcgcta cgatggctgc cacgcggact gagcgcgagg aggcagcatg cgaaccctga
     5341 tcgccaacgg cacgatcgtg accgccgacg gctcgtacga cgctgacgtc ctcgtagacg
     5401 gcgagaccat cgcgcagatc ggcagcggcc tggccgcgag cggggtcacc gccgacgaga
     5461 cgatcgacgc cgtgggccgg tacgtgatcc cgggcgccat cgacgtgcac acccacatgg
     5521 agctgccgtt cgggggaacg ttcgccaagg acacgttcga gacggggacg cgggccgccg
     5581 ccttcggcgg cacgacgtgc aacgtcgact tcgcggtgca gtcgcggggc cagagcctgc
     5641 gcgcgggcct cgacgcgtgg cacgcgaagg ccgagggcaa cgccgtcgtc gactacggct
     5701 tccacatgat catgagcgac gtcaacgacg acaccctggc cgagatggac cagctcgtct
     5761 cggagggcgt gcccgacttc aagctgttca ccgcctatcc cggcgtgttc tacagcgacg
     5821 acgccgcgat cttccgggcg atgcagcaga ccggcaggaa
//
LOCUS       DBNHXO010000230         2035 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3832867, whole genome shotgun sequence.
ACCESSION   DBNHXO010000230 DBNHXO010000000
VERSION     DBNHXO010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3832867"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="AC162A_04830"
     CDS             <1..561
                     /locus_tag="AC162A_04830"
                     /inference="COORDINATES: protein motif:HMM:NF019998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5010199.1"
                     /translation="GTTMVYVTHDQVEAMTMGQRIAVMSEGLLQQVGTPQQLYDSPIN
                     RFVAGFIGSPSMNFIELDVPAGAGAELVQGSTRFPVPAHYREGLAAAGNKVIAGFRPE
                     HLELTEIPAPFASMQGTADVVEYLGNEELLHVTVDGREVVAIVDSSHRVRPGDVVQLR
                     LPAEKLHLFAVDSGLSVAKLREVAAA"
     gene            650..1219
                     /locus_tag="AC162A_04835"
     CDS             650..1219
                     /locus_tag="AC162A_04835"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5010200.1"
                     /translation="MSDPLEERVVASELIHRGRYMEFRVDTIERADGSRGTRDVVGHP
                     GAVAVLALDDADRLLMVRQWRVPAGRALLEIPAGTLDVHDGVTEDPDGAARRELEEET
                     GHRAATWRKLASFWTAPGFASELMHLYLATGIAGVGASDDRLSPDEDERLELRHVPLD
                     EAVAIVENGELGDAKSILGILWLDRLRRA"
     gene            complement(1458..1865)
                     /locus_tag="AC162A_04840"
     CDS             complement(1458..1865)
                     /locus_tag="AC162A_04840"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5010201.1"
                     /translation="MDEETGLPADDQESAYDLLQRGQGLLRRRHNAQAAIVLERAARL
                     EPGKASILEALGRAYFNSAQIEPARRTFEALIELDPSAPYAHFALGQSLKRLGCDREA
                     WTHLRLAVALAPSSDLYRSALARMPDPRRDPAG"
BASE COUNT          285 a          736 c          733 g          281 t
ORIGIN      
        1 gggaccacga tggtctacgt cacccacgac caggtcgagg cgatgacgat gggccagcgg
       61 atcgcggtca tgagcgaggg cctgctccag caggtcggca cgccgcagca gctgtacgac
      121 agcccgatca accgcttcgt cgccggcttc atcggcagcc cgtcgatgaa cttcatcgag
      181 ctggacgtcc ccgccggcgc aggcgcggag ctggtccagg gctcgacccg cttcccggtc
      241 ccggcccact accgcgaggg cctggcggcc gccggcaaca aggtgatcgc cggcttccgg
      301 cccgagcacc tcgagctcac tgagatcccg gccccgttcg catcgatgca gggcacggcc
      361 gacgtcgtcg agtacctcgg caacgaggag ctgctccacg tcaccgtcga cggccgcgag
      421 gtcgtcgcga tcgtcgactc gtcccatcgc gttcggccgg gcgacgtggt ccagctgcgg
      481 ctcccggccg agaagctgca cctgttcgcc gtcgattccg gcctgtccgt ggccaagctg
      541 cgcgaggtgg ccgccgcctg acggcggtcg cgcccacgcg ccattcagtg cgccaatcga
      601 agccggcccg ccgccgcgac gggccggctt cctgctgatc ggagaccgca tgtccgaccc
      661 cctcgaggaa cgcgtcgtcg cgtccgagct gatccaccgc gggcggtaca tggagttccg
      721 cgtcgacacc atcgagcgcg ccgacggctc gcgcgggacg cgcgacgtcg tgggccaccc
      781 gggtgccgtg gcggtcctgg cgctcgacga cgccgaccgg ctgctgatgg tccgccagtg
      841 gcgcgtcccc gccggccgcg ccctgctcga gatcccggcg ggcacgctcg acgtgcacga
      901 tggcgtcacc gaggaccccg atggcgccgc gcgacgcgag ctggaggagg agaccggtca
      961 ccgcgccgcc acgtggcgca agctcgcctc gttctggacg gccccggggt tcgcctcgga
     1021 gctgatgcac ctgtacctgg cgacggggat cgccggcgtc ggagcctccg acgatcgcct
     1081 ctcgcccgac gaggacgagc gcctcgagct gcgccacgtg ccgctcgacg aggcggtggc
     1141 gatagtcgag aacggcgagc tcggcgacgc gaagtcgatc ctcgggatcc tgtggctcga
     1201 ccggctgcgc cgggcgtagg cacgttcccc gctggcgccg aggtatgcct ccaaggcaac
     1261 gaaacgaggt ccggggctgg ataacggcgt cgccgcgagc gtgcttccca ggcaacactt
     1321 ggggccccaa gacccccaca ttggctttct cggagcgttc ccgtgttctg gaggcacgga
     1381 taggcgacga gcggcggtcc acgcagcgct tcgacccgaa accgttgccc ggcaggcaca
     1441 acgttggcgc ggcgcgatca gccggccggg tcgcgcctcg ggtccggcat ccgcgccagc
     1501 gcggagcggt agaggtccga cgagggtgcc agggcgaccg cgagacggag gtgcgtccac
     1561 gcctcccgat cacagccgag acgcttgagg ctctggccca gcgcgaagtg cgcgtatgga
     1621 gccgacggat cgagctcgat cagggcctcg aaggtccgcc gtgcgggctc gatctgggcg
     1681 gagttgaagt acgcccgacc aagcgcctcg aggatggacg ccttgcccgg ctcgagccgc
     1741 gcggcccgtt cgagcacgat cgcggcctgc gcgttgtggc gtcgccggag cagaccctgg
     1801 cctcgctgga gcaggtcgta cgcggactcc tgatcgtccg ccggcaggcc cgtctcctcg
     1861 tccatcgggc gatcctgcca tggaccgtga gatcgcgtcg tcacggggct ttgacgccca
     1921 ggcccgtcag ccggggggcg gggccggcgg tccgggcggc tcgggcggcg gggccatcca
     1981 acccggttcg gccggggcga ccaccgccat cgcgacctgc cggtgtcgac ggcgg
//
LOCUS       DBNHXO010000231         2402 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2045976, whole genome shotgun sequence.
ACCESSION   DBNHXO010000231 DBNHXO010000000
VERSION     DBNHXO010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2045976"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..1152
                     /locus_tag="AC162A_04845"
                     /pseudo
     CDS             19..1152
                     /locus_tag="AC162A_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024241.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1165..1239
                     /locus_tag="AC162A_04850"
     tRNA            1165..1239
                     /locus_tag="AC162A_04850"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1197..1199,aa:His,seq:gtg)
     gene            complement(1327..1962)
                     /locus_tag="AC162A_04855"
     CDS             complement(1327..1962)
                     /locus_tag="AC162A_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HIR5010202.1"
                     /translation="MRIRRSIAGLLAAGALASVVAAPVAAADPPSVADVLAADGQTFD
                     NNWSDYDIVERAALTVLGAKPGSAVAALTDANASLTVFAPTDRAFRKLVHSLTGERYD
                     TEKKTFNKLVKAVTGLVGQSKTIDTIEAVLLYHVVGANVPSSAVPGLDGQVVTTAGGG
                     TFKVVVDTSVRLIDKDPDAANAHLILDRLDIPAGNSVIHTVNRVLRPINLP"
BASE COUNT          289 a          815 c          868 g          430 t
ORIGIN      
        1 ccgatcccgt ggctgggtct gggcgcggcg accgcgacgg tcgggatcgc gctcggcgtc
       61 tacaacccgt tcatcgccgt cacgctgtcg gcgaagggct tcgacgtcgc cacgatcggg
      121 atcctggccg cgctcgcctc gatcgggttc gtcgtcggca cgcccatctg ggggcagctc
      181 gccgacacgc gcttcgggcg accgcgggcc ctcgcgatcg ccggggccgc cggcggcctc
      241 atcctggcga cgatctggtt cccggtaccg gcggcgttgg tcgcgctgct gttccttgcc
      301 gggtcgctgt tcctcacagc ctggctgtca ctggtcgatg cgatcgtggt caacgcggtc
      361 aacgacagcc gccggtacgc gcgcctgcgc ctgatctcga gcttcaccta cgcggtcagc
      421 gcgctggtcg gcgggatcgt ctacgcctcc accgggtttg agctgtcgct catcctcgtc
      481 gcggtcggcg gcgtcgggct ggccttcgtg gcgctccgcc tgccggacgt cgcccgcgct
      541 cgcgccgagc ctggtgcagg ccgctcgcct tcgtggtggt cgacggcgac ggcgccgctg
      601 cgcctcctgc gcggcacgcc gggcctgccg ctggcgctgc tggcgatcgc cctcgcgttc
      661 ctcggctaca tggccggcaa cacgttcatg ggcctgttcc tgctcgagct cggcggcaac
      721 gccacggacg tcggcttcgc gtcggcgatc tccgcggcga tcgaggtccc gaccatcctc
      781 gccgcgggga tcgtggccta ccggttcggc atccgaatcc tgttcgtgat cgccgccacg
      841 tccgccgcca tcgcggttgc gagctgggcg atcgcccccg ggatcccctt cgtcctcgtc
      901 agtcgcctgc tcgcgggcgt cggctacggc gggatgctcg tcgccggggt catggccgtg
      961 caccacttcc tgccgccgga gcgccaggcg accgggcagg gcctgttgca ggcgacgtgc
     1021 ttcggggcct cggcggtgat cgtcaacgtg ctcggcggcc agctgcacgc cgtgatcggg
     1081 tatcgcgggc tgttcgcggc ctgcgccgtg gtcggcctgg tcggcatcgt catcggctgg
     1141 cgggccctcc cggggcgacc cttggtggcc ttagctcaat ggcagagcat cggattgtgg
     1201 ctccgaaggt tgcgggttcg agccccatag gccacgccaa cccgtcgacc gacgcgtccg
     1261 ggaatccgaa cggccgggcc tcgatggccc ggccgttgct cggctgcgcg tgggtgggga
     1321 gaccgatcag gggaggttga tcgggcgcag gacgcggttg acggtgtgga tcacgctgtt
     1381 gccggcgggg atgtcgagcc ggtcgagaat caggtgggcg ttggcggcat cgggatcctt
     1441 gtcgatgagc ctgacgctcg tgtcgacgac gaccttgaac gtcccgccgc cggccgtggt
     1501 cacgacctgg ccgtcgagcc ccggaacggc cgacgagggg acgttcgcgc cgacgacgtg
     1561 atagagcagg acggcctcga tcgtgtcgat cgtcttggac tggccgacca gtccggtcac
     1621 ggccttgacg agcttgttga aggtcttctt ctcggtgtca taccgctcgc cggtcagcga
     1681 gtggacgagc ttgcgaaacg cacggtcggt gggcgcgaag accgtcagtg acgcgttggc
     1741 gtcggtgagg gctgcgacgg cgctcccggg cttggcgccc aggacggtga gggcggcgcg
     1801 ctcgacgatg tcatagtcgc tccagttgtt gtcgaaggtc tggccatcgg cggccaggac
     1861 gtcggcgacg ctcgggggat cggcggcggc gaccggggcc gctacgacgc tcgccagggc
     1921 gcccgcggca agcaggccgg cgatcgaacg acgaattcgc atgttgcgta ctcctccttg
     1981 acccggacgg gcggtgtttg gtgcccttgt ccaaggtttg tttgggtcgt gtactggagc
     2041 atggtgccca accgcccact gtttgtcaaa tgttcgttca atgtgcggta caccgttgtc
     2101 caaggtttgg cggaccgctc gggcgcctcc ccactcgcga gcaccaggac catccgggag
     2161 aaccgctgcg ggggcgttga cgaacggagg gctagactgg ccgacgaccc gggtcgagcc
     2221 ggagaccagt tctcaggcag ctttcaggtt cgcggtccac catcggggcg gcatccatgt
     2281 gcgggatccg ttcctgcatc ggatcgttcc acggagtccg agcttgccga tgaccgaccg
     2341 gctggacccg ccgcccctcg ccgaaccggc cccgcccgcg gatgggcgcc ggcgcacgac
     2401 cg
//
LOCUS       DBNHXO010000232         3912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2641104, whole genome shotgun sequence.
ACCESSION   DBNHXO010000232 DBNHXO010000000
VERSION     DBNHXO010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2641104"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="AC162A_04860"
     CDS             <1..240
                     /locus_tag="AC162A_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5010203.1"
                     /translation="FVDGVPTDAFAGLPGVSDVVVEDHTLRLRVSGPITPVVQAAARY
                     ELLDFVSREPSLEETFLAQYGHASEAEAPAEPVAS"
     gene            237..1934
                     /locus_tag="AC162A_04865"
     CDS             237..1934
                     /locus_tag="AC162A_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010204.1"
                     /translation="MTAVGQAQPAFVPPKARPWSRVYGLGTVYAKTLRDSRLAVIIVG
                     GIIAALFLSSGAAFGEAYTTPESRQELVNLVNSLPPVMSGVYGNAFPVNIETLGGSIG
                     WKSGASMALIVALWSVFAMAGTLASEARRGSLEFIATTPLGMRRIAVEKVTAHLTGMA
                     IVVAVTALSAWAAGAAFHTLPGDEIPPQAAIGFALWVGLVALASGGLAFALAQVVGRG
                     AAAGIAGAITLVGYFVNGYQAAVPAFGGVANLTWWGWTAHHQPLGGAFDWATLIPVAI
                     VAIVLFIAGVELFARRDLGLTSRIPWPSTPTSLMGLGGPGTRSLGERWPLAVSWGIGI
                     GLMGFIFGAASLSLTSALDSLSPETQQIYKTIFPNIDLNGAGAFLQLAFVTFGLILVG
                     FAVATLVKGWASDETGGRLETLLSTPLSRARWAIAGGAGLFGAIVVMTLVIMIGIGIG
                     SGIAGGDVATPIVGTVVLGLYGLALAGIGMAVGGVVSTSFAGEVIAAIVIITFLIDLI
                     APALKWPDWIHQIALTSHLGQPMIGNWDWVGIAACVVIAIGGLLLGAWGISRRDIER"
     gene            1981..2877
                     /locus_tag="AC162A_04870"
     CDS             1981..2877
                     /locus_tag="AC162A_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR5010205.1"
                     /translation="MDPLALGFIALAAVLHAAWNILLKAADDPLRTAAVGVAAASVVL
                     VPIVAIGWLTLGRPSVPPQAWALGITSGGVEVLYFAFLAAAFRRGDLSVVYPLARGSA
                     PLLAIAIGVVILGERLAPGAWLGVGLLLAGLLLVQRPWRLLRSATSANDRAAAGFALL
                     TGATIAIYSALDRVGVQQTPVWVYAGILWPTCAIGLAVLVLARRRVAGGRYAVTEAPI
                     DRRTAVAGGFLTFSAYALVLAALSRAPLAIVAPLRESAVVLTSLWGVFRLREVTGRRE
                     ISIRIAGSVLVLLGAAVLALAR"
     gene            2894..>3912
                     /gene="fgd"
                     /locus_tag="AC162A_04875"
     CDS             2894..>3912
                     /gene="fgd"
                     /locus_tag="AC162A_04875"
                     /EC_number="1.1.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015746290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase
                     (coenzyme-F420)"
                     /protein_id="HIR5010206.1"
                     /translation="MQPTTPTAKPALKLGYKASAEQFHPRRLLDLAIVAEDAGFESVW
                     TSDHFQPWRHTGGHAPNALVWLGAAAASTKHVTIGTSVLTPSFRYNPAVVAQAFATLS
                     CLAEGRIILGVGTGESMNEVPVGIDWPEQSERFARLREAITLIKQLWAEEFVNFEGEY
                     FRTHDATVYDRPTIPVPIYVAASGPAAARLAGRIGDGFICTSGKGEELYTQTLLPSLA
                     EGAEKAGRDVTGMERMIEMKVSFDPDFDQAMEDTKIWAALALPGDKKVGVHDPREMEI
                     LAREAEPYAHKRWLVSSDPDEHVEQIAPYIGWGFNHLVFHFPGDAQEEAIRRYGETIL
                     PRLRAR"
BASE COUNT          489 a         1425 c         1369 g          629 t
ORIGIN      
        1 ttcgtggacg gcgtgcccac cgacgccttc gccggcctgc cgggagtatc ggacgtcgtc
       61 gtcgaggacc acacgctgcg cctccgggtc tccgggccaa tcacgcccgt cgtccaggcc
      121 gcggcccgct acgagctgct cgacttcgtg agccgcgagc ccagcctgga agagacgttc
      181 ctcgctcagt acggccacgc ctccgaggcc gaggcgcccg ctgagccggt ggcgtcatga
      241 ccgccgtcgg ccaggcccag cccgccttcg tcccgcccaa ggcccgaccc tggagccggg
      301 tctacggtct gggcaccgtc tacgcgaaga ccctgcgcga ctcgcggctc gccgtgatca
      361 tcgtcggcgg catcatcgcg gccctcttcc tctccagcgg cgcggcgttc ggcgaggcct
      421 acaccacgcc cgaatcgcgc caggagctcg tcaacctcgt caacagcctg ccgccggtga
      481 tgtccggcgt gtacggcaac gcattccccg tgaacatcga gaccctcggg gggtcgatcg
      541 gctggaagtc aggggcgtcg atggccctga tcgtcgccct gtggtccgtg ttcgcgatgg
      601 cgggcacgct cgcctcggag gcgcgacgcg gcagcctcga gttcatcgcg accaccccgc
      661 tcggcatgcg ccggatcgcc gttgagaagg tgacggcgca cctgaccggg atggcgatcg
      721 tcgtcgcggt gaccgccctc agcgcgtggg ccgccggcgc cgcgttccac acgctaccgg
      781 gtgacgagat cccgccccag gcggcgatcg ggttcgcgct ctgggtcggg cttgtcgcgc
      841 tggcatcggg cggcctcgcc ttcgccctcg cgcaggtcgt cggccgcggc gccgcggcgg
      901 gcatcgccgg tgcgatcacg ctggtgggct acttcgtgaa cggctaccag gcggccgtgc
      961 cggcgttcgg cggcgtcgcc aacctcacct ggtggggctg gaccgcgcat caccagccgc
     1021 tcgggggcgc cttcgactgg gcgacgctga tccccgtcgc gatcgtcgcc atcgtgctgt
     1081 tcatcgccgg cgtcgagctg ttcgcgcgcc gcgatctcgg cctgacctcg cggattccgt
     1141 ggccgtcgac cccgacctcg ctgatgggcc tgggcggacc cggcacccga tcgctgggcg
     1201 agcggtggcc gctggccgtg tcgtggggca tcgggatcgg cctcatgggt ttcatcttcg
     1261 gagccgcatc gctgtcattg acgagcgcgc tcgacagcct ctcgcccgag acacagcaga
     1321 tctacaagac gatcttcccg aacatcgacc tcaatggtgc cggtgcgttc ctgcagctcg
     1381 cgttcgtcac gttcggtctc atcctcgtcg ggttcgccgt cgcgacgctg gtcaagggct
     1441 gggcatcgga cgagaccggt ggccgcctcg agacgctgct ctcgacgccg ctgtcgaggg
     1501 cgcgctgggc gatcgccggg ggcgctggcc tgttcggcgc gatcgtcgtc atgaccctgg
     1561 tcatcatgat cggaatcggc atcgggtcag gcatcgcggg cggcgacgtg gccacgccga
     1621 tcgtcggcac ggtggtcctg ggcctctacg gactcgccct ggccgggatc gggatggccg
     1681 tcggcggggt cgtcagtacc tcgttcgccg gagaggtgat cgcggcgatc gtcatcatca
     1741 cgttcctgat cgacctcatc gcccccgcgc tcaagtggcc ggactggatc caccagatcg
     1801 cgctcacctc ccacctcggc cagccgatga tcggcaactg ggactgggtc gggatcgcgg
     1861 cgtgcgtggt gatcgcgatc ggcgggctgc tgctcggagc gtggggcatc tcgaggcgcg
     1921 atatcgagcg ctagccgaac gggcccggcg acgggccgcg cgcccggtag catcgcgggc
     1981 gtggacccgc tcgccctggg cttcatcgcg ctcgcggcgg tcctccacgc cgcctggaac
     2041 atcctgctca aggcggccga cgaccccctg cggactgccg ccgtcggcgt cgcggcggcg
     2101 tcggttgtgc tggtgccgat cgtggcgatc ggctggctga ccctcggccg gccgtccgtc
     2161 ccgccccagg cgtgggcgct gggcatcacg tcgggtggcg tcgaggtcct gtacttcgcg
     2221 ttcctcgccg ccgcgttccg gcgcggcgac ctctcggtcg tgtacccgct ggcgaggggc
     2281 agcgcgccgc tgctcgccat cgcgatcggc gttgtgatcc tcggggagcg gcttgctccc
     2341 ggcgcgtggc ttggcgtcgg actcctgctc gccgggctgc tgctggtcca gcggccatgg
     2401 cgcctcctcc gttccgcgac cagcgccaac gaccgggcgg cggcgggctt cgcgctgctc
     2461 accggcgcaa cgatcgcgat ctactcggcc ctcgaccggg tcggcgtcca gcagacgccc
     2521 gtctgggtct acgcggggat cctgtggccg acgtgtgcga tcggcctcgc cgtcctggtg
     2581 ctggcgcgcc ggcgcgtcgc gggcggccgg tacgcggtga cggaggcgcc catcgatcgg
     2641 cggacggccg tcgccggcgg gttcctcacc ttctcggcct acgcgctggt cctcgccgcg
     2701 ctcagccggg caccgctcgc gatcgtcgcg cccctgcgcg agtcggcggt cgtcctgacg
     2761 tcgctgtggg gcgtgttcag gctccgcgag gtcaccgggc gccgcgagat ctcgatccgg
     2821 atcgccggct ccgtcctcgt cctcctgggt gccgcggtgc tcgcgctcgc gcgctgatgc
     2881 aacaatcgga gccatgcagc ccacgacccc gaccgcgaag cccgcgctga agctcggcta
     2941 caaggcgtcc gccgagcagt tccacccccg tcgtctgctc gacctcgcga tcgtcgccga
     3001 ggacgccggc ttcgagtcgg tctggaccag cgaccacttc cagccctggc ggcacacggg
     3061 cggtcacgcg ccgaacgcgc ttgtgtggct cggggcggcg gcggcgtcga cgaagcacgt
     3121 caccatcggc accagcgtcc tcacgccgtc gttccgctac aacccggcgg tcgttgccca
     3181 ggcgttcgcc accctcagct gcctcgccga gggccggatc atcctgggcg tcgggaccgg
     3241 cgagtcgatg aacgaggtcc cggtcgggat cgactggccc gagcagagcg agcggtttgc
     3301 gcgcctgcgc gaggccatca cgctgatcaa gcagctgtgg gccgaggagt tcgtcaactt
     3361 cgagggcgag tacttcagga cccacgacgc caccgtctac gaccggccga ccatccccgt
     3421 tcccatctac gtcgccgcct caggtccggc agcggcccgc ctcgccgggc gcatcggcga
     3481 cggcttcatc tgcaccagcg gcaagggcga ggagctgtac acccagacgc tcctgcccag
     3541 cctcgcggag ggggccgaga aggccggtcg cgacgtcacg ggcatggagc gcatgatcga
     3601 gatgaaggtc agcttcgacc ccgacttcga tcaggcgatg gaggacacca agatctgggc
     3661 cgcgctcgcg ctgccgggcg acaagaaggt tggcgtccac gacccgcgcg agatggagat
     3721 cctggccagg gaggccgagc cgtacgcgca caagcgctgg ctcgtctcct cggacccgga
     3781 cgagcacgtc gagcagatcg cgccgtacat cggctggggc ttcaaccacc tggtgttcca
     3841 cttcccgggc gacgcccagg aggaggcgat ccgccgctac ggcgagacga tcctgccgag
     3901 gcttcgcgcc cg
//
LOCUS       DBNHXO010000233         4664 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2840872, whole genome shotgun sequence.
ACCESSION   DBNHXO010000233 DBNHXO010000000
VERSION     DBNHXO010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2840872"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="AC162A_04880"
     CDS             <1..624
                     /locus_tag="AC162A_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5010207.1"
                     /translation="IDLPGHGERADEPFSMAAASDAVIAAIEAEVPSGRAVVVGLSLG
                     GYVAIEAAERCPDRVTGIVLSGCSAEPVGPVGALFRALAWGLDHAPKGPLDAANRGYF
                     RMRYGRAIADPIIAGGFWATGGAEALRVLARTRFVERLARLWTPVLVINGALDPVFGP
                     GGDPWAAACRRGRHVVVSRAMHLANLDRPATFAALVAAFAADPAGTS"
     gene            724..1602
                     /gene="galU"
                     /locus_tag="AC162A_04885"
     CDS             724..1602
                     /gene="galU"
                     /locus_tag="AC162A_04885"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIR5010208.1"
                     /translation="MRVRKAVFPAAGWGTRFLPATKAQPKEMLPLVDKPIIQYAVEEA
                     VAAGIEQVIIVTSSQKRAIEDHFDLNYELEHLLEEKGEIEKLRQVRHISDLAQVAYVR
                     QKEQLGLGHAVLMAKDLIGHEPFAVILPDDVVVSDRPCIGQLIHAYQQHHGSVVAVME
                     VPPEETGRYGVIGGEPVDDGGDPDRTFRVTSIVEKPEPGAAPSNLAVIGRYVLTPKIF
                     DKLEQTQRGAGGEIQLTDAIQALMEEQAVFGYAFEGQRYDAGTTMGWLQASVELALAR
                     PDIGSEFREYLRSLDI"
     gene            1680..3596
                     /gene="pknB"
                     /locus_tag="AC162A_04890"
     CDS             1680..3596
                     /gene="pknB"
                     /locus_tag="AC162A_04890"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HIR5010209.1"
                     /translation="MTEIGSTLGGRYRLLELLGQGGMATIYRARDAQLERDVAVKLLR
                     PEFGQDPDFLARFRDEARSAASLSHPNIVPVFDFGEDESGPYIVMELVEGQDLASILR
                     DNGPLAPRQAAHIAADVARALQVAHYKGIVHRDVKPSNILIGRDGRVHVADFGIARAM
                     TDSSVTLPGTTMGSVNYFSPEQARGEPTTAASDIYSLGIVLFESLTGQRPFSGDGAAA
                     IAMARLTTTPPRPSALRSSVPPELDAIVTRAMALDPDQRYASAAAMASALEGFLTAAD
                     DKPAAAGAAAVGAAAALAATQARPAPIPYPPSAYARPPAAPPTVSTQGTPPPPPVPGE
                     GDPDEEESSNVWAWVAGIAGIIVLLLIGFLLFRFLTGGGSASPSAPPSGPPVTVPTFV
                     GLDLTAAEEQAATLGITITVSGTEESSLPPDQIISQDPPAGTAVPAGSAVTVVTARGK
                     IAVVVPDLRAKPESEAVQLIVQAGLTPGIRTDAFDPSIPVGAIIAQDPGANIVVAPGA
                     PVDYVVSKGPEPTPSPTPAPTPTPTPTPAPTPTPTPTPTPTPPPTPTPTPGPSNVGDY
                     VCMTLEVATTTIDDDGFALGDVTADPPDTDPIPSEWIVSDQKPNAGVKKAFGTPIDLV
                     LSDPETVVCPQGAG"
     gene            complement(3593..3847)
                     /locus_tag="AC162A_04895"
     CDS             complement(3593..3847)
                     /locus_tag="AC162A_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010210.1"
                     /translation="MAAGPPVADTADGVARDPDRRLSPLFEADRTPARTFVPVDELAG
                     LERAVPPATADGGPATDDALVTAGAATGPNGWSLWGDPEP"
     gene            complement(4108..>4664)
                     /locus_tag="AC162A_04900"
     CDS             complement(4108..>4664)
                     /locus_tag="AC162A_04900"
                     /inference="COORDINATES: protein motif:HMM:NF020700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010211.1"
                     /translation="EDGSSTAKNTVTIKGKTQAGSSVRVRNDENGATTTVDANKDGLF
                     EARIAIAAGLNALLITTTDPAGNPNEANLTIRKGLGKTTARLTATSYHFRSNKPPKSV
                     TFTVVVTDPDGDPVADAVALFTVTVPGLEAIVSSEIKLNENGAARFTTSIPKGALPGS
                     GLATVLVDTKEFGNVTDRQVLTVR"
BASE COUNT          648 a         1732 c         1627 g          657 t
ORIGIN      
        1 atcgatctgc ccggacacgg cgagcgggcg gacgagccgt tctcgatggc cgccgcgagc
       61 gacgcggtca tcgccgcgat cgaggccgaa gtgccgtcgg gccgcgcggt cgtcgtcggg
      121 ctctcgctgg gcggctacgt cgcgatcgag gccgccgaac gctgccccga ccgggtcacg
      181 ggcatcgtgc tctcgggttg ctcagccgag ccggtcggac ccgtcggggc gctgttccgc
      241 gcgctggcat ggggcctcga ccacgcgccg aaggggccgc tggacgccgc gaatcgcggc
      301 tacttccgga tgcgctacgg ccgtgcgatc gcggatccca tcatcgcggg cggcttctgg
      361 gcgaccggcg gcgccgaggc cctgcgggtc ctggcccgaa cgaggttcgt ggagcggctc
      421 gcccggctgt ggacgcccgt gctggtgatc aacggggcgc tcgacccggt gtttggaccg
      481 ggcggcgatc cgtgggcggc cgcctgccgg cgcggccgcc acgtcgtggt ctcgcgggcg
      541 atgcatctcg ccaacctcga ccggcccgcc accttcgccg cgctcgtcgc ggcgttcgcc
      601 gccgaccccg ccggcacctc ctgagggatc ggccccggtc gaccggcgcc ctcccgcacc
      661 ggctgggctg gaggagcccg ccggtatact ggccacacac cctccccttg ctcgagaggt
      721 tcgatgcgcg tccgcaaagc tgtcttcccg gctgccggct ggggcacgag gttcctgcca
      781 gccaccaagg cgcagccgaa agagatgctg ccgctggtcg acaagccgat catccagtac
      841 gcggtcgagg aagcggttgc cgcgggcatc gagcaggtca tcatcgtcac ctcgtcccag
      901 aagcgggcga tcgaggatca cttcgacctc aactacgagc tcgagcacct gctcgaggag
      961 aagggtgaga tcgagaagct gcgccaggtg cggcacatca gtgacctcgc gcaggtcgcc
     1021 tacgtgcgcc agaaggagca gctcggcctc ggccacgcgg tcctgatggc caaggacctg
     1081 atcgggcacg agccgttcgc ggtgatcctc cccgacgacg tggtggtctc ggatcggccg
     1141 tgcatcggcc agctcatcca cgcctaccag cagcaccacg ggtcggtggt tgcggtgatg
     1201 gaggtcccac ccgaggagac gggtcgctac ggcgtcatcg ggggcgagcc cgtcgacgac
     1261 ggcggcgacc ccgaccgcac gttccgcgtc acctcgatcg tcgagaagcc ggagcccggg
     1321 gccgcgccgt ccaacctcgc cgtgatcggc cgctacgtgc tgacccccaa gatcttcgac
     1381 aagctcgaac agacgcaacg gggcgccggg ggcgagatcc agctgaccga cgcgatccag
     1441 gcgctgatgg aggagcaggc ggtcttcggt tacgcattcg agggccagcg ctacgacgcc
     1501 ggcaccacga tgggctggct ccaggcgtcc gttgagctgg cgctcgcacg gcccgacatc
     1561 ggcagcgagt tccgcgaata cctgcggtcg ctggacatct gagcccagcc ctgcaggccc
     1621 gttagggtag gatgcgcccg gcgccgcgag gcgcgctggc cagaggagac gaccacaccg
     1681 tgaccgagat cgggagcacg ctcggcggtc ggtaccggct gctcgagctc ctcggacagg
     1741 ggggcatggc gaccatctat cgagcgcgcg acgcgcagct cgagcgggat gtcgccgtca
     1801 agctcctccg cccggagttc ggccaggacc ccgacttcct cgcccggttc cgcgacgagg
     1861 cccgctcggc ggcctcgctc agccacccga acatcgtccc cgtgttcgac ttcggcgagg
     1921 acgagtcggg gccatacatc gtcatggagc tggtcgaggg ccaggacctg gcctcgatcc
     1981 tccgcgacaa cggaccgctc gccccgcgcc aggccgccca catcgcggcc gatgtcgccc
     2041 gggcgctcca ggtggcccac tacaagggca tcgtccatcg cgacgtcaag ccgtcgaaca
     2101 tcctgatcgg gcgcgacggg cgggtgcacg tcgccgactt cggcatcgcg cgagcgatga
     2161 ccgactcctc ggtcacgctg cccggtacga cgatgggctc cgtcaactac ttcagccccg
     2221 agcaggcccg cggcgagccg accaccgccg cgtcggacat ctactcgctg ggcatcgtgc
     2281 tgttcgagtc gctgaccggc cagcggccgt tctcgggcga cggtgccgcg gcgatcgcga
     2341 tggcccgcct gaccacgacg ccgccccgcc cgtccgccct gcggtcgtcc gtgccgcccg
     2401 agctcgacgc gatcgtcacc cgcgcgatgg cgctcgatcc cgaccagcgg tacgcgtcag
     2461 ccgcggcgat ggcgagtgcg ctcgaggggt tcctcacggc tgccgacgac aagccggcgg
     2521 ccgccggggc cgccgcggtc ggtgccgctg ccgccctcgc cgcgacgcag gcccgcccgg
     2581 cgcccattcc ctatccgccg agcgcgtacg cccgcccgcc cgccgcgccg ccgaccgtgt
     2641 cgacccaggg cacgccgccg ccgcctcccg tgcccgggga gggcgatccg gacgaggagg
     2701 agagttcgaa cgtctgggcc tgggtcgccg gcatcgcagg gatcatcgtg ctgttgctga
     2761 tcggcttcct gctgttccgc ttcctcacgg gaggcggctc ggcgtccccg tccgcgccgc
     2821 cctccgggcc gccggtgacg gtgccgacct tcgtcggcct cgacctcacg gccgccgagg
     2881 agcaggccgc gaccctgggc atcacgatca ccgtcagcgg caccgaggag tcgagcctgc
     2941 cgcctgacca gatcatcagc caggatccgc ccgcgggcac ggcggtgccg gccgggtcgg
     3001 ccgtgaccgt cgtgacggcg cgcggcaaga tcgcggtggt cgtgcccgac ctgcgcgcga
     3061 agcccgagag cgaggccgtc cagctcatcg tccaggccgg gctgacgccc gggatccgga
     3121 cggacgcctt cgacccctcg atcccggtgg gtgcgatcat cgcccaggat ccgggcgcga
     3181 acatcgtcgt ggcgccgggt gcgcccgtgg actacgtggt ctcgaagggc cccgagccga
     3241 cgccgagccc gacccccgct cccacgccga cccccactcc gaccccggcg cccacgccaa
     3301 ccccgacgcc gaccccaacc ccgactccgc cgcccacgcc gaccccgacg ccgggtccaa
     3361 gcaacgtcgg cgactacgtc tgcatgacgc tcgaggtcgc gaccaccacg atcgacgacg
     3421 acggcttcgc gctgggtgac gtcacggccg atccgccgga taccgacccg atcccgtccg
     3481 agtggatcgt ctcggaccag aagccgaacg cgggcgtgaa gaaggcgttc ggcacgccga
     3541 tcgacctcgt cctctcggac ccggaaaccg tggtctgccc ccagggcgcc ggctagggtt
     3601 cggggtcgcc ccacagcgac catccgttcg gccccgtcgc ggccccggcc gtcacgaggg
     3661 cgtcgtcggt cgccggcccg ccgtcggccg tcgcgggggg gacggcgcgc tcgaggccgg
     3721 cgagctcgtc gaccgggacg aacgtgcgcg ccggagtacg gtccgcctcg aacagcggcg
     3781 acagccgacg atccgggtcg cgcgcgacgc cgtccgccgt gtccgccacg ggcggccccg
     3841 cggccaccca gcacgccctg atctcggacc cgtcgagcgc ggcgtggcgt gggtcgaggc
     3901 cgcacagccc gagtccgctg gcgattcccc gccggaagtg gagacaggtc gcacaggtcg
     3961 tcgtcgatcg ccgacagacc cagcagcgcg aactctccgc ctgcggtgta ccgcagtgcg
     4021 ggcaacgcca gaccggcatg gggcgatcgt agctgcgccg aggccgcctg tcgaggcgat
     4081 ggcgcgacga tccgaacgcg tggtcgatca gcggacggtc agcacctggc ggtcggtcac
     4141 gttaccgaac tccttcgtgt cgaccagcac cgtcgccagc ccggacccgg gcagggcgcc
     4201 cttcggaatg ctcgtggtga accgcgcggc cccgttctcg ttgagcttga tctcgctcga
     4261 gacgatcgct tcgagcccag gcaccgtcac cgtgaacagg gccaccgcat cagctaccgg
     4321 gtcgccgtcg gggtcggtca cgacgacggt gaacgtcacg gacttgggcg gcttgttcga
     4381 cctgaagtgg taggaggtgg ccgtcagcct ggcggtcgtc ttgcccagcc ccttgcggat
     4441 cgtgaggttg gcctcgttcg ggttgccggc gggatccgtg gtcgtgatca ggagcgcgtt
     4501 caggccggcg gcgatggcga tcctggcctc gaacagcccg tccttgttgg catcgacggt
     4561 cgtcgtggcg ccgttctcgt cgttgcgaac cctgaccgaa ctcccggcct gggtcttgcc
     4621 cttgatcgtg accgtgttct tggccgtgga cgagccgtcc tcgg
//
LOCUS       DBNHXO010000234         2475 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3237125, whole genome shotgun sequence.
ACCESSION   DBNHXO010000234 DBNHXO010000000
VERSION     DBNHXO010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3237125"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /gene="rsfS"
                     /locus_tag="AC162A_04905"
     CDS             <1..422
                     /gene="rsfS"
                     /locus_tag="AC162A_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR5010212.1"
                     /translation="TEPASSTAPRPDGLPARESAAPASDRAPLDIARRIVEIAEDKKA
                     ADIVLLDLGELTTLADAFVICSGGSERQISAIADGVVEGLREEGVRPIGREGTPESHW
                     VLLDYDSVIVHVFTPPEREYYQLERHWSGAKTVLRVQ"
     gene            554..808
                     /locus_tag="AC162A_04910"
     CDS             554..808
                     /locus_tag="AC162A_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010213.1"
                     /translation="MSGLRDRCPGCTRRLIITRFDTTFRLPDRTERLCFGIPGGLCED
                     CHQLYIDPELIELLDLAEGRCVFAIQSDVVLQEEAWSSAD"
     gene            complement(818..1870)
                     /gene="holA"
                     /locus_tag="AC162A_04915"
     CDS             complement(818..1870)
                     /gene="holA"
                     /locus_tag="AC162A_04915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR5010214.1"
                     /translation="MSADVPVIAYFRGDDGYTMDAAVMALAKRLEAESGTAPERWRTT
                     GAETDAASIGMRVATAPLFGGGTVAVVVDPGPLLKSKADREALEGAIRTVAPGNALVF
                     VEQGDTGSRRSAALAGLETTVLKAGGDAKQYKAPREGQLAAWIEGRAKERDMQLGPGA
                     AKELARRVGGFVHEGDVDRQRQGALAVGELEKLALYRPFDPVTEDDVRVLVSEVVPDS
                     TWAFLDAVADRKAAVAGPLLDRLLESTPEPVILVQLHRRIRELIEVADHLADGAAPGS
                     LVRTLGMKPFRVDKLVGQARRWTLAELEDALEGLLDLDAMVKGAPDSGATDAQRRLAF
                     SLWIRERVAPAALQRA"
     gene            complement(1867..>2475)
                     /locus_tag="AC162A_04920"
     CDS             complement(1867..>2475)
                     /locus_tag="AC162A_04920"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR5010215.1"
                     /translation="REAASRLRSLGPSAKFVRHATAVGDVAAFLAFHAHAAGQPINPR
                     LVETAALLHDVDKLASAGAPAHLRHGDGSAAWLEANGYPELAPVVRDHPVTRLVEPGY
                     ERWSQTASLEARIVAYADKRAAQFLAPMDQRFARWRRKYPPGSDRVVKRTGGSAWTEE
                     AFELAEQRARELELAVCAAAGVTPEQVRRLRWSRRALREAAR"
BASE COUNT          325 a          923 c          883 g          344 t
ORIGIN      
        1 tgaccgagcc cgcttcgtcc accgcccccc gaccggacgg cctgcccgcg cgcgagagcg
       61 cggcgcccgc gtccgaccgc gcaccgctcg acatcgcgcg gcgcatcgtg gagatcgcgg
      121 aggacaagaa ggcggccgac atcgtcctgc tcgacctggg cgagcttacg acgctggccg
      181 acgcgttcgt catctgctcg ggcggctcgg aacggcagat cagcgccatc gccgacggcg
      241 tcgtcgaggg cctgcgcgag gagggcgtcc gcccgatcgg ccgcgagggc accccggaat
      301 cgcactgggt gctgctcgat tacgactcgg taatcgtcca cgtgttcacg ccgccggagc
      361 gcgagtacta ccagctcgag cgccattggt caggcgccaa gacggtgctg cgcgttcagt
      421 agcctcggtt ggccccgaac gcggccggcg ggtactcccg cgctaggtac ccctgggcca
      481 tgtcagcgcc gcaccaccgt tcctagcatc ccgggcaagt cgtccgtgtg gtcgacaagc
      541 ccaaggggac ccgatgtcgg gcctgcgaga tcgctgccca ggttgcaccc ggcgcctgat
      601 catcacccgt ttcgacacga cgttccggct tcctgaccgg accgagcgcc tgtgcttcgg
      661 gatcccgggt gggctgtgcg aggactgcca ccagctgtac atcgaccccg agctgatcga
      721 gctgctcgac ctggccgagg gccggtgcgt gttcgcgatc cagagcgacg tcgtcctgca
      781 ggaagaggcc tggtcctccg cggactgacc gcccggccta tgcgcgctgc agcgcggccg
      841 gtgccacccg ctcccgaatc cagaggctga acgccagccg tcgctgcgcg tccgtcgccc
      901 ccgaatcggg cgctcccttg accatcgcgt cgaggtcgag caggccctcg agcgcatcct
      961 ccagctccgc gagcgtccag cggcgcgcct ggcccaccag cttgtcgacc cggaagggct
     1021 tcatcccgag cgtgcgcacg aggctgcccg gggccgcgcc gtcggcgagg tggtcggcga
     1081 cctcgatcag ctcgcggatc cgccggtgga gctggacgag gatgacgggc tcgggcgtcg
     1141 attccagcag ccgatcgagg aggggacccg cgacggcggc cttgcggtcg gcgaccgcgt
     1201 cgaggaacgc ccacgtcgaa tcgggcacga cctccgagac gagcacgcgg acgtcgtcct
     1261 cggtcaccgg gtcgaagggc cggtacaggg cgagcttctc gagctcgccg accgcgagcg
     1321 cgccctggcg ctggcgatcg acgtcgcctt cgtggacgaa gccgccgacg cggcgggcga
     1381 gctccttcgc ggcgcccggc cccagctgca tgtcgcgctc cttcgcgcgc ccctcgatcc
     1441 aggccgcgag ctggccctcg cgcggcgcct tgtactgctt cgcgtcgccg cccgccttga
     1501 gcaccgtcgt ctccaggccc gcgagcgctg ccgaccggcg cgagcccgtg tcgccctgct
     1561 cgacgaacac gagggcgttg ccgggcgcga cggtccggat ggccccctcg agcgcctcgc
     1621 ggtcggcctt ggacttgagc aacggtccgg ggtccacgac gaccgcgacc gtgccaccac
     1681 cgaacagtgg tgccgtcgcg acccgcatgc cgatcgacgc ggcgtcggtt tccgcgccgg
     1741 tggtccgcca ccgttcgggg gccgtgcccg actcggcctc gaggcgcttc gccagggcca
     1801 tgacggcggc gtccatcgtg tagccatcgt cgccgcggaa gtaggcgatg accgggacgt
     1861 cggcgctcat cgtgcggcct cgcggagggc gcgccgcgac caccgcaggc gcctgacctg
     1921 ctcgggcgtc accccggccg ctgcgcacac ggccagctcg agctcccggg cgcgctgctc
     1981 ggcgagctcg aacgcctcct cggtccatgc cgacccgccg gttcgcttga cgacgcggtc
     2041 cgagccggga gggtacttgc ggcgccagcg ggcgaagcgt tggtccattg gcgcgaggaa
     2101 ctgggcggcc cgcttgtccg cgtaggcgac gatccgggcc tcgagggaag cggtctgcga
     2161 ccagcgttcg tatccgggct ccaccagcct cgtcaccggg tggtcgcgga ccaccggcgc
     2221 gagctccggg tagccattgg cctcgagcca ggccgccgac ccgtcgccgt ggcggaggtg
     2281 ggccggcgcc ccggccgagg ccagcttgtc gacgtcgtgg agcagcgccg ccgtctcgac
     2341 cagccgcggg ttgatcggct gccccgccgc atgggcatgg aacgcgagga aggcggcgac
     2401 gtccccaacg gccgtggcgt gccgcacgaa cttcgccgac gggccgagcg agcgcagccg
     2461 gctggcggcc tcgcg
//
LOCUS       DBNHXO010000235         1996 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3038956, whole genome shotgun sequence.
ACCESSION   DBNHXO010000235 DBNHXO010000000
VERSION     DBNHXO010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3038956"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="AC162A_04925"
     CDS             <1..198
                     /locus_tag="AC162A_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010216.1"
                     /translation="VALSTEVHPELLAYGTGHDVRCWLYHDATGAEKAEPVLAAQGTS
                     DVIPGLVADTADAAAAEAGAS"
     gene            237..1370
                     /locus_tag="AC162A_04930"
     CDS             237..1370
                     /locus_tag="AC162A_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5010217.1"
                     /translation="MRADDEAPLVEVRDLVKHFPIRGGFLNREVAVVQAVSGVSFEVR
                     RGETLGLVGESGSGKTTVGRLLLRLIEPTSGTIKFDGTDITTIKGSKLKPYRRRMQII
                     FQDPYASLDPRTPIGESIGEGLRIHSIGTPAERAAKVSRMMDLVGLMPYHARRYPHEF
                     SGGQRQRIGIARALVVEPDLVVCDEPVSALDVSIQAQVLNLLKTLQRELDLTYLFVAH
                     NMGVVEHICDRVAVMYVGKIAEVTDRVSMFSSPLHPYTQALLSAIPVPDPEQRRKRVI
                     LKGDIPSPVRPPSGCRFNPRCQLRAGLGGPMRCAEEEPVLLEAAPPPGSHLVACHFRG
                     AGALGEAAIAELPADWGAGSDGLGPVTEPASPGSGGSPTANAG"
     gene            1589..>1996
                     /locus_tag="AC162A_04935"
     CDS             1589..>1996
                     /locus_tag="AC162A_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5010218.1"
                     /translation="MGRIEALEARTVRFTLCSPDGAFPARVAHPALGVLDAAAIDRIA
                     TNPNSVRSLAGTGPYRIDAWTPGENVRLVRADPDTSPAAEVPTLVLRWAADPTQRTIG
                     LQSATVDGIDAPGPLDLDRIATLPELAVTPRDGL"
BASE COUNT          260 a          763 c          710 g          263 t
ORIGIN      
        1 gtcgcgctgt cgaccgaggt gcatcccgag ctgctcgcct acggcacggg gcacgacgtc
       61 cgctgctggc tgtaccacga cgcgacgggt gccgagaagg ccgagcccgt cctcgccgcc
      121 cagggcacga gcgacgtgat ccccgggctc gtcgcggata cggcggacgc cgcggcggcc
      181 gaggcgggcg cctcgtgacg gtcgtcgaca cgggctccgt gccggctgcg cgccgcgtga
      241 gggcggatga cgaggcgccg ctcgtcgagg tccgtgacct cgtcaagcac ttcccgatcc
      301 ggggcgggtt cctcaaccgg gaggttgccg tcgtccaggc cgtgtccggg gtgagcttcg
      361 aggtccgccg cggcgagacg ctggggctcg tgggcgagtc gggctcgggc aagaccacgg
      421 tcgggcggct gctgctgcgg ctgatcgagc cgacgtcggg cacgatcaag ttcgatggca
      481 ccgacatcac gacgatcaag ggcagcaagc tcaagcccta ccgccgccgg atgcagatca
      541 tcttccagga cccgtacgcg tcgctcgacc cgcggacgcc gatcggcgag agcatcggcg
      601 aggggctgcg catccattcg atcgggacgc cggccgagcg cgccgcgaag gtcagccgga
      661 tgatggacct cgtcgggctc atgccctacc acgcccggcg ctacccgcac gagttctcgg
      721 gcggccagcg ccagcggatc gggatcgcgc gcgccctggt cgtggaaccg gacctggtcg
      781 tgtgcgacga gccggtgtcg gcgctcgacg tgtcgatcca ggcccaggtt ctcaacctgc
      841 tcaagacgct ccagcgcgag ctcgacctga cctacctgtt cgtcgcccac aacatgggcg
      901 tggtggagca catctgcgac cgggtggcgg tcatgtacgt gggcaagatc gcggaggtca
      961 ccgaccgggt ctcgatgttc agcagcccgc tccatccgta cacccaggcc ctgctctcgg
     1021 cgatcccggt gccggatccg gagcagcgcc gcaagcgggt catcctcaag ggagacatcc
     1081 ccagccccgt ccggcccccg tcgggctgcc ggttcaaccc gcgctgccag ctgcgcgccg
     1141 ggttgggcgg cccgatgcgc tgcgcggagg aggagccggt cctgctcgag gccgcacccc
     1201 cgccgggcag ccacctcgtc gcctgccact tccggggcgc cggcgcgctg ggcgaggccg
     1261 cgatcgcgga gctgcccgcc gactggggcg ccggctcgga cggcctgggc ccggtcaccg
     1321 aaccggcgag cccggggtcc gggggctcgc cgaccgcgaa cgccgggtga tgcgcggcgg
     1381 cacctgcaga gccgtcgtcc tcggcctctc gttggcgctc agcgcggcgg cgtgcgacgc
     1441 cggaccggtc gtgacggccc cgccgtcggg acccagccgg acgccggacg cgtccgcgtc
     1501 ggctccggca gcgagcggcg agcccttccg gtccgtctcc tggcccaccg acggtaccgc
     1561 ctgcggcacg gacggctata ccggcgccat gggccggatc gaggcactgg aggcccgcac
     1621 ggtccggttc acgctgtgca gccccgacgg tgccttcccc gcccgcgtcg cccatccggc
     1681 gctcggcgtc ctggacgcgg ccgccatcga ccggatcgcc acgaacccga acagcgtccg
     1741 gtcgctggcc ggcaccggtc cctaccggat cgacgcctgg acgccgggcg agaacgtccg
     1801 cctcgtccgc gccgatcccg acacgtcacc ggccgccgag gtcccgacgc tggtcctgcg
     1861 ctgggccgcc gacccgacgc agcgcaccat cgggttgcag tccgccaccg tcgacggcat
     1921 cgacgcccct ggtccgctcg acctcgaccg catcgccacg ctccccgagc tcgccgtcac
     1981 gccccgcgac ggcctc
//
LOCUS       DBNHXO010000236         2362 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3039235, whole genome shotgun sequence.
ACCESSION   DBNHXO010000236 DBNHXO010000000
VERSION     DBNHXO010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3039235"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="AC162A_04940"
     CDS             <1..1071
                     /locus_tag="AC162A_04940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5010219.1"
                     /translation="PRNGGASAARNLALDQIRGTWVTFLDADDILTDIGVEALLGPTR
                     TTDPLAVIGQRVWSDGTKTWVSSLYDIPDIREPGRKSMVSAPGLVYYASATGKAFHRS
                     LVGDLRFEGRVLGDQPWALRALLRAGDRIEVVPEDVYVWLRAPTEMTDPRSITATTRS
                     TARGSADAVRVAITAHAQVSAEAYRTIDDPAAQRLVARVYLERLLRSDLGMYLKRALD
                     RHDPAIAELLVAFGAFLESVPRDLLAEEGPLVTRTLLKPLVDAWLRLPPDARNAFWGV
                     VRPTVAADPSVYDKLGGGRVGRTAIRLVSAVDTAPTRAFATAATDRAAQARPHGSSPR
                     SAPPAVRAPDGDDEPAQAGSNR"
     gene            complement(1068..>2362)
                     /locus_tag="AC162A_04945"
     CDS             complement(1068..>2362)
                     /locus_tag="AC162A_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CgeB family protein"
                     /protein_id="HIR5010220.1"
                     /translation="YADAAIVIDDTATPTLPYGAVNSRVFDALAVGTLVITDNVMGAR
                     ELFGASLPAAAEPDEIVRLAERYLDDPEERRRLAIELRNIVIERHTYAHRAAELRAVL
                     ERWASAPRVDIAIGPPESDLAQLWGDFHFGRDVQRALHQRGLPSRLRLRPSWAVPDAA
                     AADVLIHVFGAFGPEAPPPRSGQLSIVWVISHPDLVTDEVVAPYDLVFVASDSFAARL
                     ADRLGRPVAALHQATDAERFMPRPGGPAHELLFVANSRGVRRRIVDELTPTDVDLAVF
                     GRHWTPDLLDPRYLRGENVPNEELATYYSAASIVLNDHWSDMAEQGFMSNRLYDAAAC
                     GAFVISDRVQGIDTEFDDGVVTFETGDELRALVRRYLDDPAARAAHAERARAAVLARH
                     TFAHRVDAMLAAAAEHLERRASRISVTLTESVASGGPA"
BASE COUNT          335 a          845 c          865 g          317 t
ORIGIN      
        1 ccacggaacg gcggcgcctc ggccgcccgc aacctcgccc tcgatcagat ccggggcacc
       61 tgggtcacgt tcctcgacgc cgacgacatc ctgaccgaca tcggggtcga ggccctgctc
      121 ggtccaaccc ggacgaccga tcccctcgcc gtcatcggcc agcgggtctg gtcggacggc
      181 acgaagacgt gggtcagctc cctgtatgac atccccgaca tccgcgagcc gggccgcaaa
      241 tccatggtct cggcgcccgg gctcgtgtac tacgcgtcgg cgaccggcaa ggcgttccac
      301 cggtcgctgg tcggcgacct gcgcttcgag gggcgcgtgc tgggcgacca gccgtgggcg
      361 ctgcgggcgc tgctgcgcgc gggcgaccgg atcgaggtcg tgcccgagga tgtgtacgtc
      421 tggctgcgcg cgccgacgga gatgaccgac ccgcgatcca tcacggcaac gacccgctcc
      481 acggcccgcg gcagcgccga cgccgtccgg gtcgcgatca cggcccacgc gcaggtgagc
      541 gccgaggcat accgcacgat cgacgacccg gcagcccagc gcctcgtggc ccgcgtctac
      601 ctcgagcggc tcctgcgctc ggacctcggc atgtacctca agcgggccct ggaccggcac
      661 gacccggcga tcgccgagct gctcgtggcg ttcggtgcgt tccttgagtc ggtccctcgc
      721 gacctgctcg cggaagaggg accgctcgtc acgcgaacgc tgctcaagcc cctggtcgac
      781 gcgtggctac ggttgccgcc cgatgcccgg aacgccttct ggggcgtcgt ccggcccacg
      841 gtcgcggccg atccctccgt gtatgacaag ctcggcggcg gacgcgtcgg tcgcactgcc
      901 atccgcctcg tgagtgccgt cgacacggcc ccgacccgcg ctttcgcgac ggctgccacc
      961 gaccgtgcgg cgcaggctcg accgcatggg tcgtcgccgc ggagcgcacc cccggctgtg
     1021 cgggcaccgg acggcgacga cgagccagcg caggcgggca gcaatcgcta ggccggtccg
     1081 ccgctggcga cgctctcggt cagcgtcacg gagatccgcg acgcccggcg ctcgaggtgc
     1141 tccgcagccg ccgcgagcat ggcgtcgacc cggtgggcga acgtgtgccg cgcgaggacc
     1201 gcggcgcggg cgcgttcggc gtgcgccgca cgagccgccg ggtcgtcgag gtagcggcga
     1261 acgagagcgc ggagctcgtc acctgtctcg aaggtgacga cgccgtcgtc gaactcggtg
     1321 tcgatcccct ggacccgatc ggagatgacg aacgccccgc acgcggcggc gtcgtagagc
     1381 cggttcgaca tgaacccctg ctcggccatg tccgaccagt ggtcgttgag gacgatggaa
     1441 gccgcggagt agtacgtcgc caactcctcg ttgggcacgt tctcgccgcg caggtaacgc
     1501 gggtcgagga ggtcgggcgt ccagtgccgg ccgaagaccg cgagatcgac atcggtcggc
     1561 gtcagctcgt cgacgatccg ccgccggacg ccccgcgagt tggcgacgaa caggagctcg
     1621 tgggccggcc cgcccggcct cggcatgaag cgttcagcgt cggtggcctg gtggagggcg
     1681 gcgaccggcc gcccgagtcg gtccgcgagc ctcgcggcga agctgtcgga ggcgacgaac
     1741 acgaggtcgt atggcgcgac gacctcgtcg gtcaccaggt cggggtggct gatgacccac
     1801 acgatggaga gctgaccgct gcgcggggga ggcgcttcgg gcccgaacgc cccgaagacg
     1861 tggatcagca cgtcggcggc cgcggcgtcg ggcaccgccc acgaggggcg gaggcgcagg
     1921 cgggatggca gcccgcgttg gtgcagggcg cgctggacgt cccggccgaa gtggaagtca
     1981 ccccacagct gcgcgaggtc gctctcgggc ggaccgatcg cgatgtcgac ccgcggcgcc
     2041 gacgcccagc gctccaggac ggcgcgcagc tccgccgcgc gatgggcata ggtgtgccgc
     2101 tcgatgacga tgttgcggag ctcgatcgcg aggcgccgcc gttcctcggg atcgtcgagg
     2161 taccgctcgg cgagccgcac gatctcgtcg ggctcagccg ccgccggcag cgaagcaccg
     2221 aacagctctc gcgcccccat cacgttgtcg gtgatgacga gcgtcccgac ggcgagcgca
     2281 tcgaacacgc gcgagttgac cgcgccatac ggcagcgtcg gggttgcggt gtcgtcgatg
     2341 acgatcgcgg cgtcggcgta gg
//
LOCUS       DBNHXO010000237         4813 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2841585, whole genome shotgun sequence.
ACCESSION   DBNHXO010000237 DBNHXO010000000
VERSION     DBNHXO010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2841585"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="AC162A_04950"
     CDS             <1..1179
                     /locus_tag="AC162A_04950"
                     /inference="COORDINATES: protein motif:HMM:NF014826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR5010221.1"
                     /translation="VIARFAALGVVDRVVGAGRAKASVTLLRGPNVDLMVMPPGEAGT
                     YLVHFTGSADHNIRLRGIARDRGWSLSEKGFVRIGEDGQALEGDAADLRTFPDEAAAY
                     GFLDLAFIEPELREDRGEVEAAREGRLPALITRADLRGDLHTHSEWSDGVHAIEVMAE
                     ACRRLGYAYQVLTDHSQSLAIAHGLEPERVEQERAIIAGLNARFADEEAAGILPPGAN
                     AGGFRLLHGCELEVRADGRLDYEDDLLARFDLVVASVHVARRQPRAELTRRTLNAIRS
                     PHVDVIAHPSGRMIQTRDDLDLDWDAVFEAAAATGTALEINGSPHRLDLAAERARRAV
                     SMGCLLTIDSDAHRTEELEYVRWGVDQARRAWVEPSNVLNTRPLPELLAWVAGKPSRV
                     "
     gene            1196..2029
                     /locus_tag="AC162A_04955"
     CDS             1196..2029
                     /locus_tag="AC162A_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010222.1"
                     /translation="MTPHDATADPTAVLARRELVVAAVVMVALTRAVEPADAFLVAGL
                     LPVVMLLAGVGVLHLGTADTRPFEALLVPAVLTGGAGAAIHLVPIGLGLIPVLLGFAI
                     LLDRILALELRLLGQATGATEADQSRVVLAAITTAFIAFTGIATLVPGGLAEPGGAAA
                     GGQALSEGWLVVMAVNDALVALLLGYRLALLRYGTARDAARSAVTSAIVVAVAAGAIR
                     AIDLPRLVGPALLTLVFYLWDALHGSAPARRREPRFMWEAILLAVLGVIVVAWNLRLR
                     A"
     gene            2191..3048
                     /locus_tag="AC162A_04960"
     CDS             2191..3048
                     /locus_tag="AC162A_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5010223.1"
                     /translation="MTFPEIDAAAQTPANQTRLKRQLVDQAIAQASAADWAEAAETNL
                     RLLELGPDSESENRLAKALWEQGELGAAREHYQKALALDPTNRIAERNIDRLKVLMVE
                     AGERTVAARPGSKAPVSIFVEETGKTGFAHLTNLARSAELAQVNPGDLVELVPEGNRL
                     IAISNGTRIGVVEPRVAARLLKLMADGNKYLAGVTSLGDRDVRLIIREVFQDPRNYGK
                     VSFPTAAKSTDLRPYTKGTLVREDEELEDDLEDDIEDEEIENLDRVLPPEETTDEAFV
                     EETDELEEP"
     gene            3114..>4813
                     /locus_tag="AC162A_04965"
     CDS             3114..>4813
                     /locus_tag="AC162A_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010224.1"
                     /translation="MAGRALLLRAEPGFSLPAFERFAPPPPPALVGARIEAGSAPGDI
                     VLDLFGRGGWVARAAIDRQRKGVSLEGTPLDRLLAEIVLRPPDLRHLDAAVQALAASA
                     RRESSLKASITDRFASRCATCERPVILDEITWSAETEGEDGQPVAPQPIRKHYRCPVC
                     RDQLGGGEQRQAPLDANDLRLALDRDAGDAREVLYDRFPVLEGGDTLADELLDLHTPR
                     QLSALAAILERVEGDLRAAPIAAALRLAILHALGPASRLLTSPGRVAPLRIAGGHVRL
                     PGGAHWRERNPWLAFEDGVRLVRGFVQRLESGAWGPVPARLGDDLRSLVEGSATAMLK
                     QGTSAALGALQLETSHLAEGGVRPRVRLVVGTPPLRPVTDRLAWAYHATAWVLGREAA
                     ATLPLEPLFGPAVRPSWGWQAAAVTRALRGVEPVMGRNARAVFLLEGDGPESLVACVL
                     GGVTADYRLATARLPEAGRETGGVVELVPPGSGAVPGGPRTRANVGLPALPGGAGDQD
                     VVERDRLFAPPDRVVDAPFSAVDAARAVVDATIDVLKLRGEPVSFAGLLGEILVGLDR
                     A"
BASE COUNT          613 a         1828 c         1736 g          636 t
ORIGIN      
        1 gtcatcgccc gcttcgccgc cctcggcgtc gtcgaccgcg tggtcggcgc ggggcgcgcg
       61 aaggcgagcg tcacgctcct gcgcggcccc aatgtcgacc tcatggtcat gccgcccggc
      121 gaggccggca cctacctcgt ccacttcacg ggctccgcgg accacaacat caggctgcgc
      181 ggcatcgctc gcgatcgggg ctggagcctg tccgagaagg gatttgtccg gatcggggag
      241 gacgggcagg cgctcgaagg cgatgccgcc gacctgcgca cgtttcccga cgaagccgcc
      301 gcgtacggct tcctcgacct cgcgttcatc gagcccgagc tgcgcgagga ccggggcgag
      361 gtcgaggcgg cccgcgaggg ccggctcccg gcgctgatca cgcgtgccga cctccggggc
      421 gacctccaca cccattcgga gtggagcgac ggcgtccacg cgatcgaggt catggccgag
      481 gcctgccggc ggctgggcta cgcctaccag gtcctgaccg accactcgca gagcctcgcc
      541 atcgcgcacg gcctggagcc cgaacgcgtg gagcaggaac gggcgatcat cgccgggctc
      601 aacgcccggt tcgcggacga ggaggcggcc gggatcctgc cgcccggggc caacgcgggc
      661 gggttccgcc tgctccacgg ctgcgagctc gaggtccgcg ccgatgggcg gctggactac
      721 gaggacgacc tgctggcgcg cttcgacctc gtggtggcgt cggtccacgt ggcgcgccgc
      781 cagccgcgcg ccgagctgac ccggcggacc ctgaacgcga tccgcagccc ccacgtcgac
      841 gtgatcgccc acccgtcggg gcgcatgatc cagacccgcg acgacctcga cctcgactgg
      901 gacgcggtgt tcgaggcggc cgcggcgacg ggcacggcgc tcgagatcaa tggctccccg
      961 caccgcctgg acctcgccgc cgagcgtgcc cggcgggcgg tctcgatggg ctgcctgctg
     1021 acgatcgact ccgacgccca ccggaccgag gagctcgagt acgtgcgctg gggcgtcgac
     1081 caggcgcgac gggcatgggt cgagccctcg aacgtcctca acacccggcc gctgccggag
     1141 ctgctcgcct gggtcgcggg caagccctcg cgggtctgac ataccatccg cgccgatgac
     1201 cccccacgac gcgaccgccg acccgaccgc ggtcctcgcc cgccgcgagc tcgtggtggc
     1261 cgccgtggtg atggtcgcgc tgacccgggc cgtcgaaccc gccgatgcct tcctggtcgc
     1321 gggcctgctc ccggtcgtga tgctgctggc gggcgtcggg gtcctgcacc tcgggaccgc
     1381 ggacacccgg ccgttcgagg cgctgctggt gccagccgtg ctcactgggg gcgccggagc
     1441 ggcgatccac ctcgtgccga tcggcctcgg actgatcccg gtgctgctcg gcttcgccat
     1501 cctgctcgac cggatcctcg cgctcgagct gcggctgctc ggccaggcga ccggggcgac
     1561 cgaggctgac cagtcgcgag tcgtgctggc ggcgatcacg acggcgttca tcgcgttcac
     1621 cggcatcgcg acgctggtgc cgggcgggct cgcggagccc ggcggtgcgg cggcgggtgg
     1681 ccaggccctg tccgagggct ggctcgtcgt gatggcggtc aatgacgccc tggtcgcgct
     1741 gctcctgggc taccggctgg cgctgctgcg ctacggcacg gcgagggacg ctgcgcgctc
     1801 ggcggtgacc tcggcgatcg tcgtggcggt cgcggccggg gcgatccgcg cgatcgacct
     1861 gccgaggctc gtcgggcccg cgctgctgac gctggtgttc tacctgtggg acgcgctcca
     1921 tggcagcgcc cccgcccggc gacgtgagcc acgcttcatg tgggaggcga ttctgctcgc
     1981 ggtgctcggg gtcatcgtcg tcgcctggaa cctgcgtctc cgcgcctgac gaaccgcttc
     2041 tgcgaccccc ggtgacgggc gtccccgttt gacggagcgg gccggggatg ctagaaacac
     2101 gtgcccgcga ccgatgccgc gcgagcctgc cacatccccc ggcatgcccc ggcgacggcg
     2161 cgtccagcgc accgaaagga cgacctctct gtgaccttcc ccgagatcga cgccgcagcc
     2221 cagacgcccg ccaaccagac ccggctcaag cgccagctgg tggaccaggc gatcgcacag
     2281 gcatcggccg ccgattgggc cgaggctgcc gagacgaacc tccggctgct cgagctcggc
     2341 ccggactccg agtccgagaa ccggctcgcc aaggcgctct gggagcaggg cgagctcggt
     2401 gcggcgcgcg agcactacca gaaggccctc gcgctcgacc cgaccaaccg catcgccgag
     2461 cgcaacatcg accgcctcaa ggtgctcatg gttgaggccg gcgagcggac cgtcgcggcc
     2521 cgaccgggct ccaaggcgcc cgtcagcatc ttcgtcgagg agaccggcaa gaccgggttc
     2581 gcccacctca ccaacctcgc ccgctcggca gagttggcgc aggtcaatcc gggcgacctg
     2641 gtcgagctcg ttcccgaggg caaccggctg atcgcgatca gcaacgggac ccggatcggc
     2701 gtggtcgagc cccgcgtggc cgcccgcctg ctcaagctca tggccgacgg caacaagtac
     2761 ctggccggcg tcacctcgct cggcgaccgc gacgtgcggc tgatcatccg cgaggtgttc
     2821 caggacccgc gcaactacgg caaggtcagc ttcccgaccg ccgccaagtc caccgacctg
     2881 cgcccctaca ccaagggcac gctcgtgcgc gaggacgagg agctcgagga cgacctcgag
     2941 gacgacatcg aggacgagga gatcgagaat ctcgaccgcg tcctgccgcc cgaggagacc
     3001 acggacgagg cgttcgtcga ggagacggac gagctcgagg agccgtagac tcccgcgctc
     3061 cggcgccagc ccggcgctcg cgaggcgctc ggcgggtctg cgacaatccg gcgatggctg
     3121 gccgtgcact gctcctgagg gcggaacccg ggttcagcct gccggcgttc gagcggttcg
     3181 cgccgccccc gcccccggcg ctggtcgggg cacgcatcga agccgggtcg gcacccggcg
     3241 acatcgtgct cgacctgttc gggcgcggcg gctgggtggc gcgggccgcg atcgaccgcc
     3301 agcgcaaggg cgtcagcctc gagggcacgc cgctcgaccg gttgctggcc gagatcgtcc
     3361 tccgcccgcc ggacctccgc cacctcgacg ccgccgtcca ggcgctggcc gcctccgcgc
     3421 gccgcgagtc cagcctcaag gcgtccatca cggaccgctt cgcgagccgc tgcgccacct
     3481 gcgagcggcc ggtgatcctg gacgagatca cctggtcggc cgagaccgag ggcgaagatg
     3541 gtcagccggt ggccccacag ccgatccgca agcactaccg ctgccccgtc tgccgcgacc
     3601 agctcggcgg cggcgagcag cgccaggcgc cactggacgc caacgacctc cgcctggcgc
     3661 tcgaccgcga cgccggcgac gcccgcgagg tcctgtacga ccggttcccc gtgctcgagg
     3721 gcggcgacac gctcgccgac gagctcctcg acctccacac gccacgccag ctgtcggcgc
     3781 tggccgcgat cctggagcgt gtcgagggtg acctccgcgc cgcccccatc gccgcggccc
     3841 tgcgcctcgc gatcctgcat gccctgggtc ccgcgagccg gctcctcacc tcgcccggga
     3901 gggtcgcgcc cctgcggatc gccggcggcc acgtccgcct ccccggcggc gcccactggc
     3961 gcgagcgcaa cccgtggctc gcgttcgagg acggcgtgcg cctggtccgg ggcttcgtcc
     4021 agcgcctcga gagcggcgcg tggggacccg tgccggcacg gctcggggac gacctgcgca
     4081 gcctcgtcga gggctcggcg acggcgatgc tcaagcaggg gacctccgcg gcgctcggag
     4141 cgctgcagct cgagacctcg caccttgccg aggggggtgt ccgcccgcgg gtgcgcctcg
     4201 tcgtcgggac gccaccgctt cgccccgtca ccgatcgcct cgcctgggcg taccacgcca
     4261 cggcgtgggt cctgggtcgc gaggccgcgg ccaccctgcc gctggagccg ctgttcggcc
     4321 cggcggtccg gccgtcgtgg ggctggcagg ccgcggcggt cacccgggcc ctgcgcggcg
     4381 tcgagcccgt gatgggacgc aacgcgcgag ccgtgttcct gctcgagggc gacgggcccg
     4441 agtcgctcgt cgcctgcgtc ctcggcggcg tgacggcgga ctaccggctg gcgaccgcgc
     4501 gactgcccga ggccggccgc gagacgggcg gcgtcgtgga gctcgttccg ccgggatctg
     4561 gtgccgtccc gggcgggccc cggacgcgtg cgaacgtcgg cctgccggca ctcccgggcg
     4621 gtgccggcga ccaggacgtc gtcgaacgtg accgcctgtt cgccccgccc gaccgcgtcg
     4681 tcgatgcgcc gttctcggca gtggacgcgg cgcgagccgt ggtcgacgcg acgatcgacg
     4741 tgctcaagct gcggggcgag cccgtcagct tcgccggcct gctgggcgag atcctcgtcg
     4801 gcctcgaccg ggc
//
LOCUS       DBNHXO010000238         3860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4429784, whole genome shotgun sequence.
ACCESSION   DBNHXO010000238 DBNHXO010000000
VERSION     DBNHXO010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4429784"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="AC162A_04970"
     CDS             complement(<1..602)
                     /locus_tag="AC162A_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HIR5010225.1"
                     /translation="MTAARTAGHSAATTWFAEAAAVLDRVATTQADGIETASRWCAEA
                     IASDGLVHLFGTGHSRIPVEEMFPRYGSYPGFNPIVELSMTFHTQVVGANGQRQAMFI
                     ERTPGLAEVILSNFRFGPRDVMIVFSASGHTAVPVEMARGARRRGLRVIAVTSVAQSM
                     SGEPDAEAGGRLMDEADLVLDVCSPAADATVTIAGLDTPVG"
     gene            complement(599..1657)
                     /locus_tag="AC162A_04975"
     CDS             complement(599..1657)
                     /locus_tag="AC162A_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018114319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5010226.1"
                     /translation="MREIGVGMLGSGFIGEFHAHGLRYVPAVRVAANYGAGPERREAF
                     AARFGSRPLATIEEVWADPAVDLVVVSLPNHLHIEAVRAAAAAGKAVACTKPLGRNGT
                     EAAEMLRLVEDAGVFNAYLENVVYNSDTIRMREMVEAGAIGRVTTFRAREGHSGPHAP
                     HFWDAELAGGGALLDMAAHGAECARYLFGKDVPVRDVFAWGATLVHGDRTSAEDNAVM
                     LIRFADDRVATCDVSWSSKGGLEGRFEVYGDAGRMVSDISASTIRAFIERPAGYVAEK
                     TDQDTGWVYPVPDETHVHGHDAMMRDVVESLREGRAPRETFRDGYAVNAILDAAYRSI
                     HSGHWESVPAGVPAAVGA"
     gene            complement(1657..2394)
                     /gene="deoC"
                     /locus_tag="AC162A_04980"
     CDS             complement(1657..2394)
                     /gene="deoC"
                     /locus_tag="AC162A_04980"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR5010227.1"
                     /translation="MALSIDSLTERDIAKTIDHSLLRPELDDAFVEAGCRLAAKYDVA
                     SVCVRPADVRRAAALLGGTDVAVGTTIGFPHGNHLTETKVFEAQRALADGATELDMVI
                     QIAALRSGRDADVGADIAAVVDVAHAGGAIVKVIFENAYLTDDEKIRACRLTEAAGGD
                     FVKTSTGFAPSGATHDDLRLMRANTSPHVGVKAAGGVRTLDALLAVMELGVTRIGATA
                     TETIILDFRARKAGDAPLASAGVDSGY"
     gene            complement(2636..3550)
                     /locus_tag="AC162A_04985"
     CDS             complement(2636..3550)
                     /locus_tag="AC162A_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR5010228.1"
                     /translation="MKLGFLTAPFPETPLTEVADWAAGAGFEALEIACWPVSTGPTRR
                     YAGTSHIDVANLSDARATEIVDEIASKGLSISGLGFYPNPLHPDPEHRAQVIGHLKHV
                     MVAARKMGVPLVNTFMGGDGSKNQDQNWEEALRVWPDIVSFANDQGVKITLENCPMLF
                     SYDEWPGGHNIATTPRMWRRILEQWGDTIGLNFDPSHLVLQMIDIRRFLKEFGPHVLH
                     YQAKDLMIDRDGLYENGVFSMGIGWQIPRIPGLGEVDWSVVFSELYRQGYEGDCIIEH
                     EDRRFEGTDEKVRQGFLIARDVLRPYCR"
     gene            complement(3600..>3860)
                     /locus_tag="AC162A_04990"
     CDS             complement(3600..>3860)
                     /locus_tag="AC162A_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010229.1"
                     /translation="GKAARLPGGHVEGFADTFGALFMAIYEDVLAGRPSASPRYPTFA
                     DGHDEMLVGDAIAESARIGGWVDVFRDVVPAAAPAAPAGAPR"
BASE COUNT          538 a         1361 c         1385 g          576 t
ORIGIN      
        1 ccgaccggcg tgtcgaggcc cgcgatcgtc accgtcgcgt cggcggccgg cgagcagacg
       61 tcgaggacca ggtcggcctc gtccatcagc cgtccgcctg cctcggcatc gggctcgccc
      121 gacatggact gggcgaccga ggtcaccgcg attacccgca ggccccggcg ccgggcccca
      181 cgggccattt ccaccgggac ggcggtgtgg ccgctggccg agaacacgat catcacgtcc
      241 cgcggcccga accggaagtt ggacaggatg acctcggcga gaccgggggt ccgctcgatg
      301 aacatggcct gacgctggcc gttcgccccg acgacctggg tgtggaacgt catcgacagt
      361 tcgacgatcg ggttgaagcc agggtacgag ccgtagcggg gaaacatctc ctcgacaggg
      421 atccgggagt gccccgtccc gaacaggtgg accagcccgt ccgacgcgat ggcctcggcg
      481 caccagcggc tggcggtctc gatgccgtcg gcctgcgtcg tcgcgactcg gtccaggacg
      541 gccgcggcct ccgcgaacca ggtcgtggcg gcggaatgtc ccgccgttct ggcggcggtc
      601 acgcgccgac cgccgcgggc acgccggccg gcaccgactc ccagtggccg ctgtggatcg
      661 agcggtaggc ggcgtcgagg atggcgttga ccgcgtagcc gtcgcggaac gtctcgcgtg
      721 gcgctcggcc ttcacggagg ctctcgacga cgtcgcgcat catcgcgtcg tggccgtgga
      781 cgtgggtctc gtccggcacg gggtagaccc agccggtgtc ctggtcggtc ttctccgcga
      841 cgtagcccgc cggccgttcg atgaacgccc ggatcgtcga tgcgctgatg tccgagacca
      901 tgcggccggc gtcgccgtag acctcgaacc ggccctcgag cccgcccttc gaggaccacg
      961 acacgtcgca cgtcgccacc cggtcgtcgg cgaaccggat cagcatcacg gcgttgtcct
     1021 cggcgctggt ccggtcaccg tggacgagcg tcgcccccca ggcgaacacg tcgcgcaccg
     1081 ggacgtcctt gccgaacagg tagcgggcgc attcggcgcc gtgggccgcc atgtcgagca
     1141 gcgcgccgcc gcccgcgagc tccgcgtccc agaagtgcgg cgcgtgcggg ccgctgtggc
     1201 cctcgcgggc gcggaacgtg gtcacccggc cgatcgcccc ggcctcgacc atctcgcgca
     1261 tccggatcgt gtcgctgttg tagacgacgt tctcgaggta ggcgttgaac acgcctgcgt
     1321 cctcgaccag gcgcagcatc tccgcggcct cggtcccgtt ccggcccagg ggcttggtgc
     1381 aggcgaccgc cttgccggct gcggcggcgg cccggacggc ctcgatgtgc aggtggttgg
     1441 gcagggagac gacgacgagg tcgacggccg ggtcggccca gacctcctcg atggtcgcga
     1501 gaggccggct gccgaagcgg gccgcgaacg cctcgcggcg ctccgggccg gccccgtagt
     1561 tggcggcgac gcggaccgcg ggcacgtagc gcaggccgtg cgcgtggaac tcgccgatga
     1621 acccggagcc gagcatcccg accccgatct cgcgcatcag tagccgctgt ccacgccggc
     1681 cgacgcgagc ggcgcgtcgc cggccttgcg ggcgcggaag tcgaggatga tcgtctctgt
     1741 cgccgtggcg ccgatccgcg tcacgccgag ctccatgacc gcgagcagcg catcgagggt
     1801 gcggacgccg ccggcggcct tgaccccgac gtggggcgag gtgttggcgc gcatcaggcg
     1861 caggtcgtcg tgggtcgccc cggacggcgc gaatcccgtg ctggtcttga cgaagtcgcc
     1921 acccgcggcc tcggtcaggc ggcaggcgcg gatcttctca tcgtcggtga gataggcgtt
     1981 ctcgaagatg accttcacga tcgcgccgcc ggcgtgggcg acgtcgacca ccgcggcgat
     2041 gtcggccccg acgtcggcgt cgcgacccga cctcagggcg gcgatctgga tgaccatgtc
     2101 gagctcggtc gcgccgtcgg cgagcgcccg ctgcgcctcg aacaccttgg tctcggtcag
     2161 gtggttgccg tgcgggaacc cgatcgtggt ccccacggcg acgtcggtcc cccccagcag
     2221 cgccgccgcc cgtcgcacgt cggccggccg cacgcacacg gacgccacgt cgtacttcgc
     2281 ggccagccgg cagcccgcct cgacgaacgc gtcgtccagc tccggccgca gcaggctgtg
     2341 gtcgatcgtc ttggcaatat cccgctcggt caggctgtcg atgctcagcg ccatgctcgc
     2401 tccggttcag tgggaccgcc gaaaccggcg attgttcgtt ccttccgaac gctgctcgcg
     2461 cgctgggcga cactcgcggc cttgatcgcg gcgggagtcg attcagtcag ccgaatccag
     2521 ctaaccgatg ggccgcgcac gtcgcccgtc gtgggcacaa cgcccggcga cggccgtgcg
     2581 ggaggcgcgc tgttcggaag atccgaagag tcggggttcc ggaccgcggc gagcgtcatc
     2641 ggcagtaggg ccgcaggacg tcgcgcgcga tcaggaaccc ctgcctgacc ttctcatcgg
     2701 tcccctcgaa gcggcggtcc tcgtgttcga tgatgcagtc gccttcgtag ccctggcggt
     2761 acagctccga gaacacaacg gaccagtcga cctcgccgag ccccgggatg cgcgggatct
     2821 gccagccgat gcccatcgag aacacgccgt tctcgtacag gccgtcgcga tcgatcatca
     2881 ggtccttcgc ctggtagtgg aggacgtgcg gcccgaactc cttgaggaac cggcggatgt
     2941 cgatcatctg gagcacgagg tgcgaggggt cgaagttgag cccgatcgtg tcgccccact
     3001 gctcgaggat ccggcgccac atccgcggcg tcgtcgcgat gttgtggcca cccggccact
     3061 cgtcgtagct gaacagcatc gggcagttct cgagcgtgat cttcacgccc tggtcgttcg
     3121 cgaagctgac gatgtcgggc cagacgcgga gggcctcctc ccagttctgg tcctggttct
     3181 tcgatccgtc gccgcccatg aacgtgttca cgagcgggac gcccatcttc cgggccgcca
     3241 ccatcacgtg cttgaggtgc ccgatcacct gcgcccggtg ctccgggtcg gggtggagcg
     3301 ggttggggta gaagccgagg cccgagatcg agaggccctt cgacgcgatc tcgtcgacga
     3361 tctcggtcgc ccgcgcatcg gacaggttgg cgacgtcgat gtggctcgtg cccgcgtagc
     3421 ggcgggtggg accggtcgag acgggccagc aggcgatctc gagggcctcg aagccggcgc
     3481 ccgcagccca gtcggcgacc tcggtgagcg gcgtctcggg gaacggggcg gtcaggaagc
     3541 cgagcttcat cgcggcgggt cctccgtcgg tcggcggcag cacacggaac gggtcgcggt
     3601 catcgggggg cgccggcggg agcggcgggg gcggcggccg ggaccacgtc gcggaagacg
     3661 tccacccagc caccgatacg agcactctcg gcgatcgcgt cgcccacgag catctcgtcg
     3721 tggccgtccg cgaaggtcgg gtagcgcggc gaggccgacg gccgaccggc gaggacgtcc
     3781 tcgtagattg ccatgaacag cgcgccgaac gtgtcggcga agccctccac gtgaccgccg
     3841 ggcagcctcg cggccttgcc
//
LOCUS       DBNHXO010000239         1668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5024535, whole genome shotgun sequence.
ACCESSION   DBNHXO010000239 DBNHXO010000000
VERSION     DBNHXO010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5024535"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="AC162A_04995"
     CDS             <1..843
                     /locus_tag="AC162A_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5010230.1"
                     /translation="EGEIKVSDFGASFQEALAQDTTQLTGIGSPAYMSPEQIRLEEVT
                     HQTDIYSLGVVMYKMLTGRLPFNASNQLSLTYEILNVAPPVPSQLRPELPALLDEICL
                     KAMRKSAHDRYDTWIDFGKDLSKAFSALRLAGETISDSAKFTELRTLSFFEDFDDVTL
                     WEALRISAWRTIPKGAVIIREGDSGESFYLLVEGEVDVSINRTSINTVRRGGCFGELL
                     YFSDKVAHRTTTITANTDITVVEIKTIALKVASSACQVSFQKAFMRVLIDRLTQANAK
                     LASR"
     gene            complement(855..>1668)
                     /locus_tag="AC162A_05000"
     CDS             complement(855..>1668)
                     /locus_tag="AC162A_05000"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR5010231.1"
                     /translation="ALEDELGVPLFDRTSSGMLLTTSGQRLLSEAEKVLAAAHVLRNE
                     ARALKGEVAGKASVGTVLEPELSRVGDFLSVMVERYPLLQLELHHEISGAALEKVRNG
                     ALDASFFYGDLAEGPVAGVALRPLVYRVAAPAAWRARIAGASWSDVAALPWVLTPPIS
                     SHNRLASALFRKHGVEPTKVVEADQESVISSLVASGVGLSLMREDVALEREAAGEICL
                     WDQGRATTTLWFIHLRERAEDPVIRALVDGLRDTWALREERAEPFQKRAARR"
BASE COUNT          273 a          609 c          546 g          240 t
ORIGIN      
        1 gagggcgaga tcaaggtgag cgatttcggg gcgtccttcc aggaggcgct cgcccaggac
       61 acgacccagc tcaccggcat cggctccccg gcgtacatgt cgccggagca gatccgcctc
      121 gaggaggtca cgcaccagac cgacatctac tcgctcggcg tcgtgatgta caagatgctg
      181 accgggcggc tgccgttcaa cgcctccaac cagctctcgc tcacctacga gatcctgaac
      241 gtcgccccgc cggtgccgtc gcagctccgg cccgagctcc ccgccctcct cgacgagatc
      301 tgcctcaagg cgatgcggaa gagcgcgcac gaccgctacg acacctggat cgacttcggc
      361 aaggacctct cgaaggcgtt ctcggcgctg cggctggcgg gcgagacgat ctccgactcg
      421 gccaagttca ccgagctccg cacgctctcg ttcttcgagg acttcgacga cgtgaccctg
      481 tgggaggcgc tgcggatcag cgcctggcgg accatcccca agggcgcggt gatcatccgc
      541 gagggcgact cgggcgagag cttctatctc ctcgtcgaag gcgaggtcga cgtgtcgatc
      601 aaccgcacgt cgatcaacac cgtgcgccgc ggcggctgct tcggcgagct gctctacttc
      661 agcgacaagg tcgcgcaccg gacgaccacc atcaccgcca acaccgacat cacggtcgtg
      721 gagatcaaga cgatcgcgct caaggtcgcg agctcggcct gccaggtaag cttccagaag
      781 gcgttcatgc gggtgctgat cgaccgcctg acgcaggcga acgccaagct ggcgtcgaga
      841 taggcccgcg cgcctcagcg gcgcgcggcg cgcttctgga acggctcggc ccgctcctcg
      901 cgcaaggccc aggtgtcgcg caatccgtcg acgagcgccc ggatcaccgg atcctccgcc
      961 cgctcgcgca ggtggatgaa ccagagcgtc gtggtggcgc ggccctggtc ccagaggcag
     1021 atctcgcccg ccgcttcccg ctcgagcgcg acgtcctcgc gcatcagcga cagcccgacg
     1081 ccggaggcga cgagcgagct gatgacggac tcctggtcgg cctcgacgac cttcgtcggc
     1141 tcgacgccgt gcttgcggaa cagcgcgctc gcgaggcggt tgtgcgagct gatcggcggc
     1201 gtgaggaccc acggcagcgc cgcgacgtcc gaccagctgg ccccggcgat ccgcgcgcgc
     1261 catgcggccg gcgcggcaac ccggtagacg agcggacgca gcgcgacgcc ggcgaccggg
     1321 ccttcagcga gatcgccgta gaagaagctc gcgtcgagcg cgccgttgcg caccttctcc
     1381 agcgccgccc ccgagatctc gtggtgaagc tcgagctgca gcagcggata gcgctccacc
     1441 atcacgctca ggaaatcgcc gacccgcgag agctccggct cgagaaccgt ccccacggag
     1501 gctttccccg cgacctcgcc cttcagggca cgggcttcgt tccgcagcac gtgggcggcc
     1561 gccaggacct tctcggcctc gctgaggagc cgctggccgc tggtggtcag cagcatcccg
     1621 ctcgacgtcc ggtcgaacag cgggacgcct agctcgtcct cgagcgcc
//
LOCUS       DBNHXO010000240         1812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5422261, whole genome shotgun sequence.
ACCESSION   DBNHXO010000240 DBNHXO010000000
VERSION     DBNHXO010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5422261"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="AC162A_05005"
     CDS             complement(<1..182)
                     /locus_tag="AC162A_05005"
                     /inference="COORDINATES: protein motif:HMM:NF015953.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR5010232.1"
                     /translation="MKGFIITLVVTAIAIVIVTYILPSVQFKDPSDHIPQVVILAVLI
                     GLVNSFIKPIVGLLSFP"
     gene            296..>1812
                     /locus_tag="AC162A_05010"
     CDS             296..>1812
                     /locus_tag="AC162A_05010"
                     /inference="COORDINATES: protein motif:HMM:NF017745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HIR5010233.1"
                     /translation="MTAFADLVSYFLDELFVLQPDIATAVGDHRYDDQWPDTSEAGRQ
                     ARLAFADRWQSTFASLDDASLSRDDRIDRGLILGELDAAVFGETVLREETWSPMTWLY
                     LLGSGLHPLLAREFAPLHERLVSVIGRLEGIPAIVADARAVTGSLPDRPVATLHAEVA
                     GRRIGGVADLGRQAVETAEAAAPDDPLVAVLLPRLRSAADAAAEALEGFGQWLTDEVA
                     PVASGGSAIGEALFAEKLRHTLREPDLTPAAVLARAESEFAAVRAEMVRIARDAWPTW
                     RSGEAVPDDDGALVRGVLDAIAADHPAADGLVTFSRDELVRVEAFCREHELITLVDEP
                     LQIDWTPEFMRSFGGAMLDSPGPLDVGQQTFFSITPPPDDWSPELTESYLREMNNRQM
                     SLLVIHEAVPGHYLQMAYANRGSSLARRVFRSGLFAEGWAVYVTQVMLDAGYGDGDPA
                     LWLNHWKYYLRCVTNAILDVRIHTMGMTSEEAVALMVDGAFQERAEAVQKDERARL"
BASE COUNT          254 a          632 c          657 g          269 t
ORIGIN      
        1 gggaacgaga gcaacccgac gatgggcttg atgaagctgt tcacgaggcc gatgaggacc
       61 gcgaggatca cgacctgggg gatgtggtcc gacgggtcct tgaactgcac ggacggcagg
      121 atgtaggtca cgatcacgat cgcgatcgcg gtcaccacca gggtgatgat gaaacccttc
      181 aagcctcgtc ctccgcgcga tcgacggttc ggccggcggc cacggccggg cgccggtgcc
      241 aatcatggca gacgggcggg ctagactcgg cgtcgtcgtc ccaactggag tgcccatgac
      301 cgcgttcgcc gacctcgtgt cgtacttcct cgacgagctg ttcgtcctgc agcccgacat
      361 cgcgaccgcg gtcggcgatc accgctacga cgaccagtgg cccgacacca gcgaggccgg
      421 ccgccaggcc cgactggcct tcgcggaccg ctggcagtcc acgttcgcga gcctcgacga
      481 cgcgtcgctc tcgcgcgacg accggatcga ccgcggcctg atcctgggtg agctggacgc
      541 ggccgtgttc ggcgagacgg tgctccgcga ggagacgtgg agcccgatga cgtggctgta
      601 tctgctgggc agcggcctgc acccgctgct ggcgcgcgag ttcgcgccgc tgcacgagcg
      661 cctcgtctcg gtgatcgggc ggctcgaagg tatcccggcg atcgtcgccg acgcccgcgc
      721 cgtcacgggc agcctgcccg atcgtccggt cgcgacgctc cacgccgagg tcgcgggccg
      781 gcggatcggc ggggtggccg acctggggcg acaggccgtg gagaccgcgg aggccgcggc
      841 gcccgatgac ccgctcgtcg cggtcctgct gccgcgcctg cggtcggcgg cggacgcggc
      901 cgccgaggcg ctcgaaggct tcgggcagtg gctgaccgac gaggtcgccc cggtggcgtc
      961 gggcggctcg gcgatcggcg aggcgctgtt cgcggagaag ctgcgccaca cgctgcggga
     1021 gcccgacctg acccccgccg cagtcctcgc ccgggccgag tcggagttcg cggccgtgcg
     1081 cgccgagatg gtccggatcg cccgtgacgc gtggccgacg tggcgatccg gcgaggcggt
     1141 gcccgacgac gatggcgcgc tggtccgcgg cgtgctggac gcgatcgccg ccgaccatcc
     1201 tgccgccgat ggcctcgtga cgttcagccg cgacgagctc gtccgggtcg aggcgttctg
     1261 ccgcgagcac gagctgatca ccctcgtcga cgagcctctc cagatcgact ggacccccga
     1321 gttcatgcgc tcgttcggcg gcgccatgct ggactcgccc ggcccgctcg acgtcggcca
     1381 gcagacgttc ttctcgatca ccccgccgcc cgacgactgg tcccccgagc tcaccgagtc
     1441 gtacctgcgc gagatgaaca accgccagat gtcgctgctc gtgatccacg aggcggtgcc
     1501 gggtcactac ctccagatgg cctacgcgaa tcgcggctcg tcgctcgccc ggcgggtgtt
     1561 ccggtcgggc ctgttcgccg agggctgggc ggtctacgtc acccaggtga tgctcgatgc
     1621 gggatatggc gatggcgacc cggccctgtg gctcaaccat tggaagtact acctgcgctg
     1681 cgtcaccaac gcgatcctcg acgtgcggat ccacacgatg gggatgacga gcgaggaggc
     1741 ggtcgcgctg atggtcgacg gcgcgttcca ggagcgggcc gaggccgtcc agaaggacga
     1801 gcgcgcgcgg ct
//
LOCUS       DBNHXO010000241         1826 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5224268, whole genome shotgun sequence.
ACCESSION   DBNHXO010000241 DBNHXO010000000
VERSION     DBNHXO010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5224268"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="AC162A_05015"
     CDS             <1..200
                     /locus_tag="AC162A_05015"
                     /inference="COORDINATES: protein motif:HMM:NF046811.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RapZ C-terminal domain-containing protein"
                     /protein_id="HIR5010234.1"
                     /translation="FLAHMRDLLDLLIPAYVEEGKTRLTIAIGCTGGFHRSIVISEEL
                     ATWLRKRDFGPVSVFHRELEK"
     gene            197..1594
                     /locus_tag="AC162A_05020"
     CDS             197..1594
                     /locus_tag="AC162A_05020"
                     /inference="COORDINATES: protein motif:HMM:NF014042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HIR5010235.1"
                     /translation="MNLRRWLTPGIGVKRWLLVAFAGLLLLAVGVAHLLRQAASDVDP
                     GSTIGRILDAVTLQWLPYQVRGLVAGVLGVTLFVYGAYRLIRALVDPFDAWDGEQPMV
                     EVIYQKRFLARGPRVVAIGGGTGLSTLLRGLKEHTSNLTAVVTVADDGGSSGVLRTQL
                     GIPAVGDIRNCIVALADAEPLMGRLLQYRFPGVGPAAGAPVRPEAAGPTPVSHDPETP
                     PAGLGGHAVGNLLLAALVELEDGDFEQAVREMNRVLAVRGRVVPATGTEITLHARLDD
                     GSEVVGQSRIANTNRVDRVWVAPDDVRPTDEALEAIADAELIVMGPGSLFTSILPALV
                     VPGIREAIAASGALVVFVCNVATQPGETGGFDLSDHIDALARHGAGHLPDIVLANNRF
                     DAHAPTGWLGQPVRLRWPPADGPQPRLALDELVDPDNAHHHDPERLATAVIGAWEREG
                     GHRRRPSVARVDRSA"
     gene            1636..>1826
                     /locus_tag="AC162A_05025"
     CDS             1636..>1826
                     /locus_tag="AC162A_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010236.1"
                     /translation="MSGSDRDLVAALRAELAAVEPSRPCDRRAEIAGLGSRLRTQEPA
                     VARLVLRLGGRSTDGSPTPA"
BASE COUNT          225 a          685 c          659 g          257 t
ORIGIN      
        1 agttcctcgc ccacatgcgc gacctgctcg acctgctgat ccccgcgtac gtcgaggagg
       61 gcaagacgcg gctcacgatc gcgatcggct gcaccggcgg cttccaccgc tcgatcgtga
      121 tctccgaaga gctcgcgacc tggctccgga agcgcgactt cgggccggtg tcggtctttc
      181 accgcgagct cgagaagtga acctccgccg ctggctgacg cccgggatcg gcgtcaagcg
      241 ctggctgctc gtcgcgtttg caggcctgct gctgctcgcg gtcggtgtcg cgcacctgct
      301 gcgccaggct gcctccgacg tcgatcccgg cagcacgatc ggccggatcc tcgacgcggt
      361 cacgctccag tggctgccgt accaggtccg cggcctcgtg gcgggcgtcc tcggcgtgac
      421 gctgttcgtc tacggggcgt accgcctgat ccgcgcgctg gtcgacccgt tcgatgcctg
      481 ggacggcgag cagccgatgg tcgaggtgat ctatcagaag cgcttcctgg cccggggacc
      541 gcgcgtcgtg gcgatcggcg gcgggacggg gctctcgacg ctcctgcggg gcctcaagga
      601 gcacacctcg aacctgaccg cggtcgtcac ggtcgccgat gacggaggct cctcgggcgt
      661 gttgcggacc cagctcggga tccccgccgt gggcgacatc cgcaactgca tcgtcgccct
      721 cgcggacgcc gagccgttga tgggccggct gctccagtac cgattcccgg gcgtcggccc
      781 agcggccggt gcgccggtcc gcccggaggc ggcggggccg acgccggtat cgcacgaccc
      841 ggagacgccg cccgctggcc tcggcggaca cgccgtgggc aacctgctcc tcgcggcgct
      901 cgtggagttg gaggacggcg acttcgagca ggccgtgcgc gagatgaacc gggtcctcgc
      961 ggtccgcggc cgggtggtcc cggcgacggg caccgagatc acgctccacg cccgcctcga
     1021 cgacggcagc gaggtcgtgg gccagagccg gatcgcgaac accaaccggg tcgaccgcgt
     1081 gtgggtagcc cctgatgacg tccgcccgac cgacgaggcg ctcgaggcga tcgcggatgc
     1141 ggagctcatc gtcatgggcc cgggcagcct gttcaccagc atcctgccgg ccctggtcgt
     1201 gcccgggatc cgcgaggcca tcgccgcgtc cggcgccctg gtcgtgttcg tgtgcaacgt
     1261 cgccacccag ccgggcgaga ccggcggctt cgacctctcg gaccacatcg acgcgctcgc
     1321 ccgccacggc gccgggcatc tgcccgacat cgtcctcgcc aacaaccggt tcgatgccca
     1381 tgccccgacg ggctggctgg ggcaaccggt gcggctgcgc tggccgcccg ccgacggccc
     1441 gcagccgagg ctcgcgctcg acgagctggt cgatcccgac aacgcccacc accacgatcc
     1501 cgaacgcctc gcgacggcgg tgatcggcgc ctgggagcgc gagggcggcc atcgccgccg
     1561 gccgagcgtg gcgcgcgtgg accgctcggc gtgacccggc cggaccgggc ctgaccccgg
     1621 ggcagccgaa gccgcatgtc cgggtccgat cgcgacctcg tggccgccct gcgggcggag
     1681 ctcgcggcgg tcgagccctc ccgaccgtgt gaccggcggg cggagatcgc ggggctgggg
     1741 tcccgcctca ggacccagga gccggccgtc gcccgcctcg tcctgcggtt gggcggccgc
     1801 tcgaccgatg gctcgccgac gcctgc
//
LOCUS       DBNHXO010000242         1794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4827313, whole genome shotgun sequence.
ACCESSION   DBNHXO010000242 DBNHXO010000000
VERSION     DBNHXO010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4827313"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="AC162A_05030"
     CDS             complement(<1..442)
                     /locus_tag="AC162A_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010237.1"
                     /translation="MSARAAAPAAMPTTFVVTLIPAAAALSIVASYVAVALGLPIYLD
                     AIGTCVIAIVLGPWWGALTGVLTNVGGAFFFGPSNIPFALANVLAALLWGYGVRTFGM
                     GRKPVTYFALCVIVGIATGAAGAVIALFVYGGATGHPSDVVTAAL"
     gene            complement(439..>1794)
                     /locus_tag="AC162A_05035"
     CDS             complement(439..>1794)
                     /locus_tag="AC162A_05035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5010238.1"
                     /translation="LDVDEIRKQVTGAVIVPGDADYDAARTVMLGGVDVHPALIVRVA
                     GTDDVRAVIALARDSGLELAVRSGGHSGAAHSTTEGGIVLDVRDLDALEIDEDERTAW
                     AGSGLTAGAYTIAAAERGLATGFGDTGSVGLGGITTGGGIGYLVRKHGLTIDSLLAAE
                     VVTADGVVHLVDDTHEPDLFWAIRGGGGNFGVVTRFKFRLHPLPSIVGGILVLPAAAE
                     TIAGFMAAAATAPDELSTIANVMPCPPMPFVPEDQHGQVVILALLCWSGPAEAGEAAL
                     APFRALATPVADLLHAGPYTEMYPPEDADYHPLAIQQTLFLDSFNRTIAQTILVHLDA
                     SDAAMRVAQLRALGGAMARVDADATAFAHRDRAFMVSVATFYESEHDRAARDAWVRGL
                     ARALHQRDGVYVNFLEDEGPARVREAYPGRTWDRLVELKRRYDPENLFRRNQNIPPAS
                     R"
BASE COUNT          276 a          647 c          628 g          243 t
ORIGIN      
        1 cgagcgcggc cgtgaccacg tccgatggat ggcccgtcgc cccgccatac acgaacagcg
       61 cgatgaccgc ccccgcggcc ccggtcgcga tcccgacgat cacgcacagc gcgaagtagg
      121 tgaccggctt ccggcccatc ccgaaggtcc ggacgccata gccccagagc agcgccgcga
      181 ggacattggc cagggcgaac gggatgttgc tggggccgaa gaagaaggcg ccgcccacgt
      241 tggtgagcac gccggtcaac gccccccacc acgggccgag aacgatcgcg atcacgcagg
      301 tcccgatcgc gtcgaggtag atcggcagac cgagggccac cgccacgtaa ctcgcgacga
      361 tgctcagtgc cgcggccgcg gggatcagcg tgacgacaaa ggtcgtcggc atcgccgccg
      421 gcgcggcggc ccgggcgctc atctcgaggc gggtgggatg ttctggttgc gacggaacag
      481 gttctccggg tcgtaccggc gcttgagctc gaccagtcgg tcccaggtcc gaccggggta
      541 ggcctcccgg acgcgagccg gaccctcgtc ctccaggaag ttcacgtaga cgccgtcgcg
      601 ctggtgcagc gcccgcgcga gtccacggac ccacgcgtca cgcgccgccc tgtcgtgctc
      661 gctttcatag aacgtggcga cgctgaccat gaacgcccgg tcccgatggg cgaacgctgt
      721 cgcgtcggca tcgaccctgg ccatcgcgcc gcccagggct cgcagctggg cgacccgcat
      781 cgccgcgtcc gacgcgtcga ggtgaacgag gatcgtctgc gcaatcgtcc tgttgaacga
      841 gtcgaggaac aaggtctgct ggatggccag cggatggtaa tcggcatcct cgggcggata
      901 catctcggtg tacgggcctg cgtggaggag gtccgcgacg ggggtcgcga gcgcccggaa
      961 cggcgcaagg gcggcctcgc ccgcctcggc cggcccggac cagcagagca gcgcaaggat
     1021 cacgacctgg ccgtgctggt cctcgggcac gaacggcatc ggcggacagg gcatcacatt
     1081 ggcgatcgtg gacagctcgt ccggcgcggt ggcggccgcc gccatgaacc ccgcgatggt
     1141 ctcggccgcc gccggcagga cgaggatgcc gccgacgatg gacggcagcg ggtgaagcct
     1201 gaacttgaag cgggtcacga caccgaagtt gcccccgccg ccgcggatgg cccagaacag
     1261 gtccggctcg tgcgtgtcgt ccacgaggtg gaccacgccg tcggccgtca cgacctctgc
     1321 ggcgagcagg ctgtcgatcg tgagtccgtg cttgcggacg aggtagccga tcccgccgcc
     1381 cgtcgtgatg ccgcccaatc cgaccgagcc cgtatccccg aacccggtcg cgagcccgcg
     1441 ctcggcggcc gcgatcgtgt atgccccggc ggtcagcccg ctgcccgccc acgcggtccg
     1501 ctcgtcctcg tcgatctcca gggcatcgag atccctgaca tcgagcacga tcccgccctc
     1561 ggtcgtgctg tgcgcggcgc cgctgtggcc gccggatctg acggccagct ccagcccgct
     1621 gtcgcgtgcg agggcgatca ccgcccgcac gtcatcggtg ccggccaccc ggacgatgag
     1681 cgcggggtgg acgtcgacgc cccccagcat cacggtccgc gcggcgtcgt agtcggcgtc
     1741 gccgggcacg atcacggcgc ccgtgacctg cttccggatc tcgtcgacgt cgag
//
LOCUS       DBNHXO010000243         6852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3835648, whole genome shotgun sequence.
ACCESSION   DBNHXO010000243 DBNHXO010000000
VERSION     DBNHXO010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6852
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3835648"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="AC162A_05040"
     CDS             complement(<1..308)
                     /locus_tag="AC162A_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010239.1"
                     /translation="MVRIRRFGIVRTANTVAAIYAVIVLVFVVIFALVALAGVAVLPR
                     GSGAAAGAGAIGILVGGLLLTGIYTIFGWIFTAIACALYNGVAGRVGGIEIQIESTTP
                     G"
     gene            complement(368..1168)
                     /locus_tag="AC162A_05045"
     CDS             complement(368..1168)
                     /locus_tag="AC162A_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-like protein"
                     /protein_id="HIR5010240.1"
                     /translation="MPRSRVLTGLAVVGLLVVAVFGVGQVFGVGPLAKAVPPDQVTDP
                     KEMVARSLQATLDASAVHFDGTVSGSIAGALVGRPEASVDLSGTTLDADIRPKDGKTR
                     THLASGGLDVDVDTVTVWDGVWYRTGPDDPWTRASLGGASAEAGVDINPLTLVDRLRS
                     YLATPGIQPTTSDVACASASGRCHEIHLDAGSDPVMILGLMLPRDQAAGLPPVDIDLT
                     LQTDALTLRPARLIADAKSADGTVDIHAVIDAKDWDGNSVIDEPVEGA"
     gene            1335..2915
                     /locus_tag="AC162A_05050"
     CDS             1335..2915
                     /locus_tag="AC162A_05050"
                     /inference="COORDINATES: protein motif:HMM:NF013959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010241.1"
                     /translation="MTTLARRLSLTTAISSLLLTMVAAPVAAAPAAAMPSDFDGDGYA
                     DLAIGVQGEDIGSEFDAGAINVLYGTSNGPAAGGDQFWSQDSPGVKGTSQGGRTAGIG
                     NGDYFGAALASGDFDKDGYADLAIGVPSDRVGHDNVRGGAVNILYGSKSGLTATGDQL
                     WSLANLPGVPVKHDWFGGALASGDFDGDGYWDLAIGAAGRDIGGVRQAGEVRLLYGGH
                     AGLTEARSHALTRSSVAGQAPSREEGFGAAVAAGDVTGDGRAELAVGAPFGTFYGSPG
                     GVGLFHGSSTGISVTGAQWWTQTSPGIDDPVDQGDRFGAALAFGDFDNDGHRDLAVGT
                     PNIEIDAAVGKVIVLPGSVAGLTTAGSQVWDSPVGGTDDWGPEFGFALAAGDFDGNGA
                     DDLAIGAPWTGSRVGSVYVLYAGGGGLDDARAQRWSQDSVGVPGTSEDDDVFGIAVTA
                     LDFDRSGHDDLVIGASNETGHRLGSGRITVIRGRAAGLTNVGIRAWTQDSRHIRDAEE
                     PWDRFGSTLTGSPGRPGW"
     gene            2912..4555
                     /locus_tag="AC162A_05055"
     CDS             2912..4555
                     /locus_tag="AC162A_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010242.1"
                     /translation="MSTRRPSARAAAALGALAILCVSIPAPSRAVAAAALPSDFNGDG
                     HADLAIGVPGESVGSAGRDAGAVNVLYGSAQGPTASGDQLWTLDSKGVKGTSHGRRNG
                     LDRRGDLFGGAIASGDFDRDGHADLAIGSENGRLPGKSRRVGIVTVLHGSRRGLTASG
                     DQLWSQAALPGTPERGDGFGASLATGDFDGDGYADLAIGVIGDDIGQLGNEGSVEILY
                     GSPKGLSSSRARVLTRSMTGAPDADVAFGAALAAGDLNGDGRDELAVGAPTCNWVAYS
                     SCTIAPGDVSVFYASTNGLVPAGSQIWSQDSPGIEDDPEEDDSFGFSLAVGDFDGDGY
                     GDLGIGVPSESVDPCSSPDPAYCDVGAVAVLYGGEGGLVAAGSQLWHVEIPGVPGEID
                     HDLFEQQVFGYAIAAGDFDGDGRDDLAFSDPWAIVDGELYAGMVIVLYGSDAGIGIDR
                     AQSWTQDSPGVPDTADGLDLFGRALTSADFGRSRNDDLVIGVPNEDLAGERDGGMVNV
                     VFGGDTGLDGAGAQGWSQLTPGIRDAPEKGDRFGAALTP"
     gene            complement(4626..6254)
                     /gene="groL"
                     /locus_tag="AC162A_05060"
     CDS             complement(4626..6254)
                     /gene="groL"
                     /locus_tag="AC162A_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904572.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR5010243.1"
                     /translation="MAKQLIFDETARRSLKRGIDRLADAVRVTIGPKGRNVVLDKKFG
                     SPTITNDGVTIARDIELEDPFENMGAQLLKEVATKTDDVAGDGTTTAVVLGQAMVAEG
                     LRVVTAGANPMVVKRGIEKAVETIVADIKKTARPVETREQIAAVAAISAADPEVGEII
                     AEVMDKVGKDGVITVEEGQSLGLEKEYTEGMQFDRGYISAYFVTNPDRMETVLEDVLI
                     LITDKKISAVPDLLPGLEKAVQLGRPMLIIAEDVDGEALATLVVNKLKGTISVLAVKA
                     PGFGDRRKEMLRDIAILTGGTVISEEIGRKLDSVTIEDLGKARRVVATKDDTTLVDGA
                     GSPEQIKARMSQIKAQIEDTTSDYDKEKLQERLAKLAGGVAVIKVGAATEVELKEKKH
                     RIEDALSTTRAAVEEGLVAGGGTTLLQAIPALDKLKLQGDEAVGVDIVRKALEAPARQ
                     IADNAGAPGEVVVSKVRGLKTGEGYDALKGEYGDMFVKGIVDAAKVTRSALQNAASIA
                     AMVLTTETLVTDIPEKDRAGAGAGHDHGGGGMDF"
     gene            complement(6309..6617)
                     /gene="groES"
                     /locus_tag="AC162A_05065"
     CDS             complement(6309..6617)
                     /gene="groES"
                     /locus_tag="AC162A_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR5010244.1"
                     /translation="MATASSTKLKPLGDRVVIKPTPREEMTKSGIVLPDTAKEKPQEG
                     EILAVGPGKVLDDGSREAIDVSVGQKVLYAKYAGTEFKVEGEELLIVSQKDILAIVED
                     "
BASE COUNT          963 a         2309 c         2462 g         1118 t
ORIGIN      
        1 ccgggcgttg tcgactcgat ctggatctcg atcccgccta cgcgtccggc cacgccgttg
       61 tacagggcgc acgcgatggc cgtgaagatc cagccgaaga tcgtgtagat cccggtcagc
      121 agcagcccgc cgaccaggat cccgatcgcg ccggctcccg cggcggcacc cgatccgcgc
      181 gggagcacgg cgacaccggc gagcgcgacc agggcgaaga tcaccacgaa gacgagcacg
      241 atgaccgcat agatcgcggc aaccgtgttg gccgtgcgaa cgatcccgaa tcgccggatg
      301 cggaccatgg gcatgctccc cgatgcggtg ggtaggtgcc cgcgagtcta cgcgcgccag
      361 gggcccctta cgccccctcg accggctcgt cgatcacgct gttgccgtcc cagtccttgg
      421 cgtcgatcac ggcgtggatg tcgaccgtcc catcggcgct cttcgcatcc gcgatgagcc
      481 gcgcgggacg cagcgtcagg gcgtccgtct ggagcgtgag gtcgatgtcg accggcggca
      541 ggcccgccgc ctggtcgcgg ggcagcatca ggcccaggat catgaccggg tcggagcccg
      601 cgtcgaggtg gatctcgtgg cagcggcccg aggcgctcgc gcaggcgacg tcgctggtcg
      661 tcggctggat tccaggggtc gcgaggtacg accgcaggcg gtcgacgagg gtgagcgggt
      721 tgatgtcgac gccggcctcg gcggacgccc ccccgagcga cgcgcgcgtc cagggatcgt
      781 ccggcccggt ccgataccag acgccgtccc agacggtgac cgtgtccacg tcgacgtcga
      841 ggccgccgct cgcgaggtgg gtccgggtct tgccgtcctt gggccggatg tcggcgtcga
      901 gcgtcgtgcc gctgagatcg actgaggcct ccgggcggcc gaccagcgcg ccggcgatcg
      961 agcccgagac ggtgccatcg aagtgcacgg ctgatgcgtc cagggtcgcc tggagcgagc
     1021 gggcgaccat ctccttgggg tcggtgacct ggtcgggcgg cacggccttc gccagcgggc
     1081 cgacgccgaa cacctgcccc acgccgaaca cggccaccac caggaggccg acgacggcga
     1141 ggcccgtcag gacgcgggat cggggcatga gggatcaaag gctccatggc gggcccgcgc
     1201 gggccccggg tcgatcagtg tcggcgtaca ggctcgggcc tgtcggccca tcggtcaaac
     1261 gggcacggtc caccgtccgc gcaacaccga gtggggcttc acgtctccaa ggcgacacca
     1321 caacaggagc gttgatgact acgctggctc gccgcctctc gctcacgacg gcgatctctt
     1381 ccctactgct cacgatggtc gccgcgcccg tcgccgcagc acctgccgcc gcgatgccct
     1441 ccgatttcga cggcgacggc tacgcggacc tcgcgatcgg cgttcagggc gaggacatcg
     1501 gctccgagtt cgacgcgggc gcgatcaacg tcctctacgg gacgtccaac gggcctgcgg
     1561 ccgggggcga ccagttctgg agccaggaca gcccgggggt caagggcact agccagggcg
     1621 gccgcaccgc cgggatcggg aacggcgact acttcggtgc cgcgctggcg tccggcgact
     1681 tcgacaagga cggctacgcg gacctcgcga tcggcgtgcc atcggaccgg gtcggccatg
     1741 acaacgtccg cggcggggcg gtcaacatcc tgtacggatc gaagtcggga ctcacggcga
     1801 ccggcgatca gctgtggtcg ctggccaacc tccccggggt gccggtcaag cacgattggt
     1861 tcggtggcgc gctggcgtcg ggcgacttcg acggcgacgg ttactgggac ctcgcgatcg
     1921 gggcagccgg gagggacatc ggcggtgtcc ggcaggcggg cgaggtccgg ctgctgtacg
     1981 gcggccatgc gggccttacg gaggcgaggt cacatgcact gacgaggtcg agcgtggccg
     2041 ggcaagcccc gtcccgcgaa gaggggttcg gagccgcggt cgccgcgggc gacgtgaccg
     2101 gcgatggtcg cgccgagctc gccgtcgggg caccgttcgg gacgttctac gggtccccgg
     2161 gtggcgtcgg cctgttccac ggcagttcga cggggatctc cgtcaccggc gcacagtggt
     2221 ggacgcagac gagccccggg atcgacgatc ccgtcgacca gggcgaccgg ttcggggccg
     2281 cgctcgcctt cggcgacttc gacaacgacg gtcatcgcga cctggccgtc ggcacgccga
     2341 acatcgagat cgatgccgct gtgggcaagg tgattgtcct ccccggctcg gtcgccggct
     2401 tgacgacggc agggtctcag gtctgggaca gccccgtggg gggaacggac gactggggtc
     2461 cggagttcgg cttcgcgctg gccgcgggcg acttcgacgg gaacggcgca gatgatctgg
     2521 cgatcggcgc accctggacc ggatcgagag tcgggagcgt ctacgtgctc tacgccggcg
     2581 gcggcggcct cgacgatgcc cgcgcccagc gctggtcgca ggacagcgtc ggggtcccgg
     2641 gcaccagcga ggacgacgac gtgttcggga tcgccgtgac ggcgctcgac ttcgatcggt
     2701 caggccatga cgaccttgtg atcggcgcgt caaacgagac aggtcaccgg ttgggttcgg
     2761 gtcggatcac ggtcatccgt ggccgggccg ccgggctgac caatgtcggg atccgcgcct
     2821 ggacccagga ctcgcggcac atccgggacg ccgaagaacc ctgggaccgc ttcgggtcga
     2881 ccctgacggg ctcccccggg agaccgggct ggtgagcact cggcggccct ccgcgcgtgc
     2941 ggcagccgcg ctcggcgcgc tcgcgatcct gtgcgtctcg ataccggccc cgtcccgtgc
     3001 ggtggctgcc gccgcactgc catccgactt caacggcgat ggtcacgcgg acctagcgat
     3061 cggcgtgccc ggcgagtccg tcggtagcgc gggtcgggac gccggcgcgg tgaacgtgtt
     3121 gtacgggtcc gcgcagggcc cgaccgcgtc tggcgaccag ctctggacgc tcgacagcaa
     3181 gggtgtgaag gggacgagcc atggccggcg caacggcttg gaccgacgcg gcgacctgtt
     3241 cggcggggcc atcgcctcgg gcgacttcga ccgcgacggg catgcggatc tcgccatcgg
     3301 gtcagagaac gggcgccttc ccggcaaatc gcgtcgcgtg ggcatcgtga cggtcctgca
     3361 cggctcccgc cgcggcctga cggcgtccgg tgaccagctc tggtcacagg cggcattgcc
     3421 gggcaccccc gagcggggcg acgggttcgg tgcatcgctc gccacgggcg acttcgacgg
     3481 tgacggatac gccgacctcg ccatcggcgt gatcggcgac gacatcggcc agcttggcaa
     3541 cgagggcagc gtcgagatcc tgtacggcag tccgaagggg ctgtcgtcat cccgcgcacg
     3601 cgtgctcacg cggtcgatga ccggtgcgcc cgatgcggac gtggcctttg gggcggcgct
     3661 tgccgccgga gatctgaatg gcgatggtcg tgacgagctc gctgtcggag ctccgacctg
     3721 caactgggtc gcgtactcct cctgcacgat cgctcccggt gacgtctcgg tcttctacgc
     3781 gtcgaccaac gggcttgtgc cggcgggcag ccagatctgg tcgcaggata gccccggaat
     3841 cgaggacgac cccgaggagg acgactcgtt cggcttctcg ctcgccgtcg gggatttcga
     3901 cggcgacgga tacggtgacc tcgggatcgg ggtgccaagc gaatccgtcg acccgtgctc
     3961 gtcgccagac ccggcatact gcgacgtcgg tgcggttgca gtgctgtacg gcggggaagg
     4021 cggtctcgtc gcggccggca gccagctgtg gcacgtcgag atcccgggcg tgcccgggga
     4081 gatcgatcac gacctgttcg agcaacaagt cttcggctac gccatcgccg ccggcgactt
     4141 cgatggcgac ggccgagacg acctcgcgtt cagtgatcct tgggcgatcg tcgacggcga
     4201 gctgtacgcg ggcatggtga tcgtcctgta cggcagcgac gccggtatcg gcatcgatcg
     4261 cgcccagagc tggacccagg acagtccggg cgtccccgac accgccgatg gccttgacct
     4321 gttcggacgt gccctcacca gcgccgactt cggcagatcc aggaacgacg acctcgtgat
     4381 cggagtcccg aacgaggatc tcgccggcga gcgtgacggc ggcatggtca acgtggtgtt
     4441 cggcggcgac accggccttg atggagcggg cgcgcaggga tggtcgcagc tcactcctgg
     4501 gatccgggac gcacccgaga aaggggaccg ctttggtgcg gcgctgacgc catagtccat
     4561 gaatcaagtg gcccccggag gctgctgcct gccgggggcc gtgtgctcgc gcgattggga
     4621 acggactaga agtccatccc tcccccgccg tggtcgtgac cggcgccggc gccggctcga
     4681 tccttctcgg ggatgtccgt cacgagcgtc tcggtggtca ggaccatcgc ggcgatcgag
     4741 gcggcgttct ggagtgccga gcgggtgacc ttggcagcgt cgacgatgcc cttgacgaac
     4801 atgtcgccgt actcgccctt gagggcgtcg tagccctcgc cggtcttgag gccgcggacc
     4861 ttgctcacga cgacctcgcc gggggcgccg gcgttgtcgg cgatctgccg ggccggagcc
     4921 tcgagcgcct tgcggacgat gtccacgccg acggcctcgt cgccctggag cttgagcttg
     4981 tcgagcgccg ggatcgcctg cagcagcgtg gtgccgccgc ccgcgacgag gccctcctcg
     5041 acggccgcac gggtcgtcga gagcgcatcc tcgatgcggt gcttcttctc cttgagctcg
     5101 acctcggtgg ccgcgccgac cttgatgacg gcgacgccgc cggccagctt ggcgaggcgc
     5161 tcctggagct tttccttgtc gtagtccgag gtggtgtcct cgatctgcgc cttgatctgc
     5221 gacatgcgcg ccttgatctg ctccggcgag ccggcgccat caacgagcgt cgtgtcgtcc
     5281 ttggtggcga ccacgcggcg tgccttgccg aggtcctcga tcgtgaccga gtcgagcttg
     5341 cggccgatct cctcggagat caccgtgcca cccgtcagga tcgcgatgtc gcggagcatc
     5401 tccttgcggc ggtcgccgaa gcccggcgcc ttgaccgcga ggaccgagat cgtgcccttg
     5461 agcttgttca cgaccagggt cgcgagcgcc tcgccgtcca cgtcctcggc gatgatcagc
     5521 atcgggcggc ccagctggac ggccttctcg aggccgggca ggaggtccgg cacggccgag
     5581 atcttcttgt cggtgatcag gatcaggacg tcctcgagga cggtctccat ccgatcgggg
     5641 ttggtcacga agtaggccga gatgtagccc cggtcgaact gcatgccctc ggtgtattcc
     5701 ttctcgaggc cgagcgactg gccctcctcg acggtgatga cgccgtcctt gccgaccttg
     5761 tccatcacct cggcgatgat ctcgccgacc tcgggatccg ccgcggagat cgccgcgacg
     5821 gccgcgatct gctcgcgggt ctcgacgggt cgggcggtct tcttgatgtc ggcgacgatc
     5881 gtctcgacgg ccttctcgat gccgcgcttg acgaccatcg ggttggcgcc ggcggtaacg
     5941 acccgcaggc cctcggcgac catcgcctgg cccaggacca ccgcggtggt ggtgccgtcg
     6001 ccggcgacgt cgtcggtctt ggtcgcgacc tccttgagga gctgcgcgcc catgttctcg
     6061 aacgggtcct cgagctcgat gtcgcgagcg atcgtgacgc cgtcgttggt gatcgtggga
     6121 gagccgaact tcttgtccag gacgacgttc cggcccttgg ggccgatcgt cacgcggacc
     6181 gcatcggcga ggcggtcaat gccccgcttc agggagcgac gagcggtctc gtcgaagatg
     6241 agctgcttgg ccaactggta gtccctccgg gatctggcgt agtggtgggg ttcgtccggt
     6301 gtttgcggtc agtcctcgac gatcgcgagg atgtccttct ggctgacgat gaggagctcc
     6361 tcaccctcga ccttgaactc ggtcccggcg tacttggcgt acaggacctt ctggccgacg
     6421 gagacgtcga tcgcctcgcg cgagccgtcg tcgaggacct tgcccggtcc gacggccagg
     6481 atctcgccct cctggggctt ctccttcgcc gtgtccggca ggacgatgcc cgacttcgtc
     6541 atctcctcac gcggtgtggg cttgatgacg acgcggtcgc ccagcggctt gagcttcgtg
     6601 gaagaggctg tggccaacgt gcaatcctcc tctggcggcc gcgaaccggc cgccaaatgg
     6661 cctgaaatgt gttgacgcgg accctccgtg gcgtgctgcc ctggctggcc cgtgtgcagg
     6721 tcgtcgagtg cgtcaggccc gtgacgccgc gtcggtcgca tccgcccgca ccggggtgtc
     6781 gtcccgtggg cggcgtcgcc agccgcagtc aggttattag cactctcgcc gttagagtgc
     6841 caatctaccg cg
//
LOCUS       DBNHXO010000244         4374 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_858375, whole genome shotgun sequence.
ACCESSION   DBNHXO010000244 DBNHXO010000000
VERSION     DBNHXO010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_858375"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..876)
                     /locus_tag="AC162A_05070"
     CDS             complement(229..876)
                     /locus_tag="AC162A_05070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024638.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0052851 - ferric-chelate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR5010245.1"
                     /translation="MKVAIIGTGNVGSALAGSFTKAGHEVTLSARDAAKTLEVTRATG
                     ATAVADASEAVAGADVVVIAVPYGATAAVAAEIASAAAGKVVIDVTNPLKSDYSGLAT
                     GDGPSGAEQLATQLVDARVVKAFNTVFAGIQGDPDGLGTTLDALYATDDAEAREVVSA
                     LAESVGFRPVYVGPLAAARELEAMAWLNIRLQMLTNGTWNSAFVLVAPPAASTEA"
     gene            1018..1593
                     /locus_tag="AC162A_05075"
     CDS             1018..1593
                     /locus_tag="AC162A_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5010246.1"
                     /translation="MGREIRRWRQTRGLTLAQVGERSGLNIGYLSQIENEKAVPSLEA
                     LAAIASAIDVPAAWLLLDSSSPPRVVRLDDRPQADGPGGALMTEVDAGTSRDVCILEV
                     AAPPGHATGVHAHHGDEHHLILSGRWRMTQGEHVVELGPGDYLAWDPSIPHDVENIGD
                     EAGRILIIYPRRGRRASAGDPGDVSQASPTR"
     gene            1599..2336
                     /gene="ribA"
                     /locus_tag="AC162A_05080"
     CDS             1599..2336
                     /gene="ribA"
                     /locus_tag="AC162A_05080"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HIR5010247.1"
                     /translation="MTLPKFDALLDRDANHDCSGYGRDHVCVRIAAAAELPTRFGDFH
                     AVGFWNPVDGKEHAAFVHGHEAIDGQAVPVRIHSECLTGDAIGSLRCDCRDQLEASLR
                     QLAVMPYGILLYLRQEGRGIGLANKIRAYALQDHGMDTVDANLALGFRDDERDYSIAA
                     HMLDSLGVGSVRLMTNNPRKIDGLRELGVDVVDRIPLVMPANRYNRRYLSTKARRSGH
                     LIDDPALEQQEPTLPRARQGEAVADVA"
     gene            2326..3039
                     /locus_tag="AC162A_05085"
     CDS             2326..3039
                     /locus_tag="AC162A_05085"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5010248.1"
                     /translation="MSPEPQVALITGATGPLGHAVVRRFAADGARVVLVGTDGGRLDA
                     AAAGATLAGDAWLPVVGDLRDPATALAAVAAAEERFGRVDVLLHLVGGWAGGTAVVDL
                     DPAEITSMLDQHLWTTLHLAQAVVPGMVSRGYGRLLAVSSPFAANPGPKGAAYAIAKS
                     AEEILLRSLAREVGGSGVTANVVVVRKIDATRERETEPGARNAAWTTPEEIANAFAFL
                     ASPEAAAINGARIPLDGRG"
     gene            3026..3715
                     /locus_tag="AC162A_05090"
     CDS             3026..3715
                     /locus_tag="AC162A_05090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HIR5010249.1"
                     /translation="MAAAEARVADASTPGRPRVTWVFGATLDGRVAAPDGTSRWISSQ
                     EARADAHRLRAAHDAVLVGSGAARTDDPHLGLRHGVDGSPPLRVVLDARGSIVSSSSR
                     VVDDAAPTLVVLGPDAEAPALPDRVAIARVPLDDRGRFDLDALLAALAARGVTTLLVE
                     GGPTIAGAFLEAGLVDDVVGYVRAGLLGAGRPLLELANVGTIADLRPLNLVAADVVGD
                     DVRIRATVPRP"
     gene            complement(3724..>4374)
                     /locus_tag="AC162A_05095"
     CDS             complement(3724..>4374)
                     /locus_tag="AC162A_05095"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR5010250.1"
                     /translation="DLGRLAAPYWTANAHKWLCAPKGVAILHVRADRRKLIRPLVISH
                     GRNSDRTDRSRFRLEFDWTGTADPSGILALPAALRYVGGLDEDGWPGFMATNRSLARR
                     GRDVLCSALGVLPPAPDVMIGSMAAVPLPNLAPTRAAAERLQVALFEEERIEVPITIF
                     PVAAAARDGAGEQALVRISAQRYNRPEEYARLADTLAKRLRGPSSPRALLGRLRRG"
BASE COUNT          588 a         1583 c         1604 g          599 t
ORIGIN      
        1 atcggtcatg tcgagggctc actcatcgag ggaacggtaa gcgcggcgat gcgtcccacg
       61 catcgtttct ggtcggtgat gtcgccgggt tgcgcggcgc gcatcagtcc gacctgatgg
      121 agccccgatt ccctgtgaat cgcgtcgtcg cgatgcgcgg cacgcatggt cgcgacaagc
      181 acgacacaag gtgatgactg ccacgcatca cgcaggcgct cgggacggtc aggcctcggt
      241 cgatgcggcg ggcggcgcga cgaggacgaa cgcggagttc caggtgccgt tggtcagcat
      301 ctggagccgg atgttcagcc aggccatcgc ctcgagctcg cgggcggcgg ccagcggtcc
      361 gacgtagacc gggcggaagc cgaccgactc ggcgagcgcg gagacgacct cgcgagcctc
      421 ggcgtcgtcg gtcgcgtaca gcgcgtcgag cgtggtcccg agcccgtcag ggtcgccctg
      481 gatcccggcg aacaccgtgt tgaacgcctt gacgacgcgg gcatcgacga gctgggtggc
      541 cagctgctcg gcgccggacg ggccatcgcc cgtggccagg cccgagtagt ccgacttgag
      601 cgggttggtg acgtcgatga cgaccttgcc ggcagcagcc gatgcgatct cggcggcgac
      661 ggcggccgtg gctccgtacg ggaccgcgat cacgacgacg tccgcgccgg cgacggcctc
      721 ggacgcgtcc gcgacggcgg tggcgccggt cgcgcgggtg acctcgagcg tcttcgcggc
      781 gtcgcgcgcc gagagcgtga cctcgtggcc cgccttggtg aagctgcccg cgagcgcgct
      841 gcccacgttg cccgtgccga tgatggcgac cttcatggtg tccgaacctc tctgcgtccc
      901 cgacgcgact cccgcggcgc ttgacgctat gccttcagca aagcactatt gctgtagtac
      961 aactctgcca cagggttggc ctaggagtca agtaggttgt ctgtcgacaa actcaacgtg
     1021 ggacgcgaga ttcgccgctg gcggcagacc cgcggcctga cgctcgccca ggtgggcgaa
     1081 cggagcgggc tcaacatcgg ctacctgtcg cagatcgaga acgagaaggc cgtcccgtcg
     1141 ctcgaggcgc tggcggctat cgcgtcggcc atcgacgtgc cggcggcctg gctgctgctg
     1201 gactcgtcct ccccgccgcg cgtggtccgc ctcgacgacc ggccccaggc cgacgggccc
     1261 ggcggcgcgc tgatgacgga ggtcgacgcc ggcacgtccc gcgacgtgtg catcctcgag
     1321 gtcgccgccc cgcccggtca cgcgaccggc gtgcacgccc accacggcga cgagcaccac
     1381 ctgatcctgt ccggccgctg gcgaatgacg cagggcgagc acgtggtgga gctcgggccg
     1441 ggcgactacc tggcctggga cccgtcgatc ccgcacgacg tggagaacat cggcgacgag
     1501 gccggccgga tcctgatcat ctacccccgg cgcggtcgcc gcgcatccgc cggggacccc
     1561 ggcgacgtat cccaggcaag ccccactcgg tgaatgtcat gacactgccc aagttcgacg
     1621 cgctcctcga ccgcgacgcc aaccacgact gcagcggcta cgggcgcgac cacgtctgcg
     1681 tgcggatcgc cgccgccgcg gagctgccca cgcgcttcgg cgacttccac gccgtcggat
     1741 tctggaaccc cgtcgacggc aaggaacatg cagccttcgt tcacggccac gaggccatcg
     1801 acggccaggc cgtgccggtc cgcatccact ccgaatgcct cacgggtgac gcgatcgggt
     1861 ccctgcgctg cgattgccgg gaccagctcg aggcatcgct ccggcagctg gcggtgatgc
     1921 cgtacgggat cctgctctac ctccgccagg agggccgggg catcggcctg gccaacaaga
     1981 tccgggccta cgcgctccag gaccacggca tggacacggt cgatgcgaac ctcgccctcg
     2041 gcttccgcga tgacgagcgc gactactcca tcgccgctca catgctcgac tcgctcggcg
     2101 tggggtccgt gcgcctcatg accaacaacc cgcgcaagat cgacgggctg cgcgagctcg
     2161 gcgtcgacgt cgtcgaccgc atcccgctcg tgatgcccgc caaccggtac aaccgccgct
     2221 acctgtcgac gaaggcgcgc aggtccgggc acctgatcga tgatccggcg ctcgagcagc
     2281 aggagccgac gctgccgcgg gcgcggcagg gcgaggcggt cgcggatgtc gcctgagccg
     2341 caggtcgcgc tgatcaccgg tgcgaccggg ccgctcgggc acgcggtggt ccgccggttc
     2401 gcggccgatg gcgcgcgtgt cgtgctggtc gggaccgacg ggggccggct cgacgccgcc
     2461 gcggccgggg cgacactggc gggcgacgcc tggctcccgg tcgtcggcga cctgcgggat
     2521 cccgccacgg ccctcgcggc tgtcgccgcg gccgaggagc gcttcgggcg ggtcgatgtg
     2581 ctgctgcatc tcgtcggcgg ctgggccggg ggaaccgcgg tcgtggacct cgaccccgcc
     2641 gagatcacgt ccatgctcga ccagcacctg tggacgacgc tccacctcgc tcaggccgtg
     2701 gtgccgggca tggtgagccg cggctacggg cgcctgctcg cggtgtcgtc gccgttcgcc
     2761 gccaacccgg ggccgaaggg tgcggcgtac gcgatcgcga agtcggccga ggagatcctc
     2821 ctgcgatcgc tcgcccggga ggtgggcggg agcggtgtca cggcgaacgt cgtggtcgtg
     2881 cgcaagatcg acgcgacgcg cgaacgggag accgagcccg gggcgcgcaa cgccgcctgg
     2941 acgacgccgg aggagatcgc gaacgccttc gcgttcctcg cctcgccgga agcggccgcg
     3001 atcaacgggg cgcggatccc gctcgatggc cgcggctgag gctcgcgtgg cggatgcgag
     3061 cacgccggga cggccccggg tgacctgggt gttcggcgcg acgctggacg ggcgggtcgc
     3121 cgcgccagac ggcacgtcgc gctggatctc gtcgcaggag gctcgcgcgg acgcccaccg
     3181 gcttcgcgcc gcgcacgacg cggtcctcgt gggcagcggc gcggcgcgga ccgacgaccc
     3241 gcacctgggc ctgcgccacg gcgtcgacgg gtcgccgccg ttgcgggtgg tgctcgacgc
     3301 ccgcggatcc atcgtgagct cctcgtcccg cgtcgtggac gacgccgccc ccacgctggt
     3361 cgtgctcggc ccggacgcgg aagcgcccgc actcccggac cgcgtcgcga tcgcgcgcgt
     3421 ccccctcgac gatcgcggcc gtttcgatct cgatgccctg ctggcggcgc tggccgcgcg
     3481 aggcgtgacg accctgctgg tcgagggcgg cccgacgatc gccggggcgt tcctcgaggc
     3541 cggcctggtc gacgacgtgg tcggctacgt gcgggcgggg ctgctcggtg cgggccgtcc
     3601 gttgctggag ctggcgaacg tgggcaccat cgccgacctg cggcccctga acctcgtcgc
     3661 cgcggatgtc gtcggcgacg acgtgcggat ccgggccacc gtcccgcgcc cctgaacgcg
     3721 ggatcagccc cggcgcaggc gcccgaggag tgcgcgcggg gaggagggcc cgcgtagccg
     3781 cttggcgagc gtgtcggcca gtcgcgcgta ctcctcgggc cggttgtagc gctgggccga
     3841 gatccggacg agcgcctgct cgccggcgcc gtcccgggcc gccgccgcca ccgggaagat
     3901 cgtgatcggg acctcgatcc gctcctcctc gaacagcgcg acctggagtc gctccgccgc
     3961 agcccgcgtc ggggccaggt tcgggagggg cacggcggcc atcgacccga tcatcacgtc
     4021 cggcgccggc ggcaggacac ccagcgccga gcacagcacg tcgcgcccgc ggcgcgccag
     4081 cgaccggttc gtggccatga agccgggcca gccgtcctcg tccaggccgc ccacgtagcg
     4141 cagggcggcg ggcaacgcga ggatcccgga cgggtcggcg gtgccggtcc agtcgaactc
     4201 gagcctgaac cgcgaccggt ccgtccggtc cgagttccgg ccgtgcgaga tcaccagcgg
     4261 ccggatcagc ttccggcggt cggcccggac atggaggatc gcgacgccct tcggcgcgca
     4321 cagccatttg tgcgcgttgg ccgtccagta cggcgccgcc agccggccga ggtc
//
LOCUS       DBNHXO010000245         1738 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2843840, whole genome shotgun sequence.
ACCESSION   DBNHXO010000245 DBNHXO010000000
VERSION     DBNHXO010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2843840"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..1718
                     /gene="nusA"
                     /locus_tag="AC162A_05100"
     CDS             342..1718
                     /gene="nusA"
                     /locus_tag="AC162A_05100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR5010251.1"
                     /translation="MSRDFVGALLQLNAEKQVSREALVRALEEGIQAAYRRVAGEEDI
                     FVRVEPDSGRIDVYRGRYVVDEIEDPMVEVTVDEARQTKDDAQAGDVIEIEQLDGEHF
                     GRIGAQTAKQIILQRIREVERDQVFDQFANREGELLTGSVSRVEPRAIILDVGKGVEA
                     ILATSEQSPLEHYRIGQPVKAYVLEVRRSARGPQILVSRTHKGFLKRLFELEVPEIHA
                     GTVEVKAIAREDGSRSKVAVSSRQEGLDPVGATVGQRGARVQAVVAELAGEKIDVIPW
                     NEDPSVFVANALSPAQVLGVKIDTEHRIANVTVPERMLSLAIGREGQNARLAARLTGW
                     RIDIRSDVAAAESPDVEMPGVNGAEAVAAEAPAVPDQTVAAPAVAAAAPSVAEAAAVE
                     APSEPAPAKAAPRKRTSAATANAPAAGAPDEPFAADASPPAEPEAATAPAKPKRTRKA
                     KVDEEVSS"
BASE COUNT          274 a          630 c          617 g          217 t
ORIGIN      
        1 ggcacgttcg agtcgccggt ggcgccggtg ttcgtcgacg gcaagctcga ccggacgctc
       61 cggggcgacc acatcgtcga cgagttcatc gccatcctcg aggactacgt cgaccgccgc
      121 tacgcccgct cagaggcgtc tgcgacggtc tgagacggcg gccgcccgga cccgcgcggg
      181 gcgacccgtt ctgagcgtgg ctgagcgtgg ctgtccacgt gcccgggtgt atacttcccg
      241 ccaataccgc gaggccgcct caaggacgtg ggtgaccaac ccacgtctct tcgtgtcagg
      301 gacgcctcgt cgccatcgag caggcagcag ggaggcacat gatgagtcgt gacttcgtcg
      361 gtgcgctgct ccagctcaac gcagagaagc aggtctcgcg agaggcgctc gtcagggcgc
      421 tcgaggaggg catccaggcc gcgtatcgcc gggtcgccgg cgaggaggac atcttcgtcc
      481 gcgtcgagcc ggacagcggc cggatcgacg tctatcgcgg ccgctacgtc gtcgacgaga
      541 tcgaggaccc gatggtcgag gtcacggtcg acgaggcgcg ccagaccaag gacgacgccc
      601 aggccgggga cgtgatcgag atcgagcagc tcgacggcga gcacttcggc cgcatcggcg
      661 cccagacggc gaagcagatc atcctccagc ggatccgcga ggtcgagcgc gaccaggtct
      721 tcgaccagtt cgcgaaccgc gagggagagc tgctgaccgg ctccgtcagc cgggtcgaac
      781 cgcgggcgat catcctcgac gtcggcaagg gcgtggaggc gatcctcgcg accagcgagc
      841 agagcccgct cgagcactac cggatcggcc agcccgtcaa ggcgtacgtc ctcgaggtgc
      901 gccggtcggc gcgcggcccg cagatcctcg tgtcgcggac ccacaagggc ttcctcaagc
      961 gcctgttcga gctcgaggtc cccgagatcc acgccggcac cgtcgaggtc aaggccatcg
     1021 cccgcgagga cggcagccgc tccaaggtgg cggtctcgtc gcgccaggag gggctcgacc
     1081 ccgtcggcgc gaccgtcggg cagcgcggcg cgcgcgtcca ggccgtcgtg gcggagctgg
     1141 cgggcgagaa gatcgacgtg atcccgtgga acgaggatcc atcggtgttc gtcgccaatg
     1201 ccctttcgcc cgcccaggtg ctgggcgtga agatcgacac cgagcaccgg atcgccaacg
     1261 tgaccgtgcc cgagcggatg ctctcgctcg cgatcggccg cgagggtcag aacgcgcggc
     1321 tcgccgcccg cctcaccggc tggcgcatcg acatccgcag cgacgtcgcg gccgccgaat
     1381 cgcccgatgt tgagatgccc ggagtcaatg gcgcggaggc cgtcgccgcc gaggcgcccg
     1441 ccgtacccga ccagaccgtt gccgcgcccg ccgtcgcggc ggctgctccg tctgtggccg
     1501 aggccgctgc cgtcgaggca ccctccgagc ccgcccccgc caaggccgcg ccgaggaagc
     1561 gcaccagcgc cgcgaccgca aacgcgcctg ccgccggcgc gcccgacgag cccttcgcgg
     1621 ccgacgcgag cccgcccgca gagcccgagg cggcgaccgc gcccgccaag cccaagcgga
     1681 cccggaaggc caaggtcgac gaggaggtgt cgtcgtagcc ccggcggccg gtcgaacc
//
LOCUS       DBNHXO010000246         2060 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1256850, whole genome shotgun sequence.
ACCESSION   DBNHXO010000246 DBNHXO010000000
VERSION     DBNHXO010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1256850"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1363
                     /locus_tag="AC162A_05105"
     CDS             146..1363
                     /locus_tag="AC162A_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5010252.1"
                     /translation="MTSASTSRAATVEFEHVTKVYGSGASKQGTPGAVNDLSLTVPAG
                     KICVLVGPSGCGKTTSLKMVNRLIEPTSGRILLDGVDAATRNVTDLRRGIGYVIQQTG
                     LFPHQTIGENVETVPRLLGWSKARRHERAEELLALVGLDPASYAGRYPAQLSGGERQR
                     VGVARALAADPPIMLMDEPFGAVDPIVRERLQNEFLRLQEQLAKTILFVTHDIDEAIK
                     MGDLVAVFRTGGIVEQFGPPSEILAAPASPFVARFVGADRGLKRLSLTRVNDLELAPA
                     VTARVGDDAADARRRTLADPFAYLLLLDADGRPLGWVGQGRIPGDGVLEADLVETTSP
                     LLDRRTTLKDTLSMLLDRDVQAGVVVDRHGVYRGLVNVDQIAAYMRDTARPFDAVDGP
                     VTAVDLAAVSGEG"
     gene            1364..1999
                     /locus_tag="AC162A_05110"
     CDS             1364..1999
                     /locus_tag="AC162A_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5010253.1"
                     /translation="MPEFLRRYGGQLADGLVDHVVLTAIAIGVGFTLSLILTLLIVRW
                     RRLGGPIIAGAGILYTIPSLALFAFLVPITGLSLLSAEIGLISYTLLILVRNTLQGLD
                     GISPDIIEAADGLGHTRTQRLLRVELPLAMPLIIAGLRIATVTTIGLVMVTAVIGEGG
                     LGQVLLRGFNLRNWGIVYAALIVMIVLAIVADGGLGFAGRLLTPWARRGSR"
BASE COUNT          282 a          748 c          718 g          312 t
ORIGIN      
        1 gagcgcctgg cgccgtacgt ggagctcggg ttcacgaccg cgatcacgga gcagccggcc
       61 ccgtacgacg acgagacgct cgagcggttc atcggccagg tgaagccgct cgtcgaccgc
      121 ggctgacccc gcggcgcgac cgccgatgac cagcgcctcg acgtcccggg ccgcgaccgt
      181 cgagttcgag cacgtcacca aggtctacgg ctcgggcgct tcgaaacagg gcacgccggg
      241 cgcggtcaac gacctctcgc tcaccgtccc ggccgggaag atctgcgtcc tcgtcggtcc
      301 ctcgggctgc ggcaagacga cgtcgctcaa gatggtcaac cggctgatcg agccgacctc
      361 gggccggatc ctgctcgacg gcgtggacgc ggcgacgcgc aacgtgacgg accttcggcg
      421 cgggatcggg tacgtgatcc agcagaccgg cctgttcccc caccagacga tcggcgagaa
      481 cgtggagacg gtcccacgcc tgctcggctg gtcgaaggcc cgccggcacg agcgggcaga
      541 agagctcctc gccctggtgg gcctggaccc cgcgagctac gcgggccgct accccgcgca
      601 gctgtccggc ggcgaacggc agcgcgtcgg cgtcgcccgg gcgctcgccg ccgacccgcc
      661 gatcatgctc atggacgagc cgttcggcgc cgtcgacccc atcgtgcgcg agcgcctcca
      721 gaacgagttc ctgcgcctcc aggagcagct ggccaagacg atcctgttcg tcacccacga
      781 catcgacgag gccatcaaga tgggcgacct cgtcgccgtg ttccggacgg gtgggatcgt
      841 cgagcagttc gggccgccgt ccgagatcct cgccgcgccc gcctcgccgt tcgtcgcccg
      901 gttcgtcggc gcggaccgcg ggctcaagcg cctgtcgctg acccgcgtca acgacctcga
      961 gctcgcgccg gcggtgacgg cgcgagtggg cgacgatgcg gccgatgccc ggcgaagaac
     1021 cctcgcggac ccgttcgcct acctcctcct gctcgatgcc gacggacgac cgctgggctg
     1081 ggtgggccag gggcggatcc cgggcgatgg cgtgctcgag gcggacctgg tcgagaccac
     1141 gtcgccgctc ctcgaccggc ggacaacgct caaggacacg ttgtcgatgc tgctcgaccg
     1201 tgacgtccag gccggggtcg tcgtcgatcg ccacggcgtc taccgcgggc tcgtgaacgt
     1261 ggaccagatc gccgcctaca tgcgcgatac cgcgcggccg ttcgacgccg tcgacggccc
     1321 ggtcaccgcc gtggacctgg cggccgtctc cggggaggga tgagtgccgg agttcctgcg
     1381 ccgctacggc ggccaactcg ccgacggact cgtcgaccac gtcgtgctca cggcgatcgc
     1441 gatcggcgtc gggttcacgc tgagcctgat cctgacgctg ctgatcgtcc gctggcggcg
     1501 cctcggcggc cccatcatcg ccggcgccgg gatcctgtac acgatcccga gcctcgccct
     1561 gttcgcgttc ctcgtcccga tcaccgggct gtcgctgctg tcggccgaga tcgggctgat
     1621 ctcgtacacg ctgctgatcc tggtccggaa cacgctccag ggcctcgacg gcatctcgcc
     1681 cgacatcatc gaggccgccg acgggcttgg gcacacgcgg acgcagcggc tgctgcgggt
     1741 cgagctcccg ctggcgatgc cgctgatcat cgccggtctg cggatcgcca ccgtgaccac
     1801 catcggtctg gtgatggtga cggccgtgat cggcgagggc ggcctcggcc aggtcctgct
     1861 gcggggcttc aacctgcgca actggggcat cgtgtacgcc gccctcatcg tgatgatcgt
     1921 gctcgcgatc gtggccgacg gcggcctcgg gttcgccggg cgcctgctca cgccgtgggc
     1981 tcgtcggggc agtcgctgat ggaggtcatc ggctggttca ccgatcccgc gaactggtcg
     2041 gggacgagcg gcatcccgaa
//
LOCUS       DBNHXO010000247         4266 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_660844, whole genome shotgun sequence.
ACCESSION   DBNHXO010000247 DBNHXO010000000
VERSION     DBNHXO010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_660844"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..885)
                     /locus_tag="AC162A_05115"
     CDS             complement(73..885)
                     /locus_tag="AC162A_05115"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR5010254.1"
                     /translation="MLLVVDIGNTNVTLALVADGVIVSTRRAGTPASATADELDVLIA
                     GLIGLDERTLADISGLAVASVVPSLTATIEIVATRRAIPLLVASAGNVPLPVRVDRPH
                     EVGADRLVNALAAARLYGTPAVVVDFGTATTLDCVGLDGAYVGGAIAPGLELGLEALA
                     SRTAKLPRIELRTPDRAIGRDTVSAMQSGTIFGYQALASGLLARVRAELAEATGTKPE
                     DVRAILTGGLSAAPWARGVEGVDAIDPDLTLKGLAILYAEVAGGAAMEAGRR"
     gene            931..2895
                     /locus_tag="AC162A_05120"
     CDS             931..2895
                     /locus_tag="AC162A_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HIR5010255.1"
                     /translation="MRLPRPGRSLSPFVIAAVVALPLVAACTAVVLATSYDERVVRNL
                     DDFFDPQVVRIDPGTTIEWVNVGRNPHTVTADDGSFDSGTLQNGQAWERRFMEPGVFP
                     YFCRLHGAPGIGMIGTIVVGDVQLPDNRGRGGVSPGREPVPTTPGTTIRVPAEAPTIQ
                     EGVNQANPGDLVLVAPGVYPETVLVRTPFLTIRGEDRNAVILDGEFKRANGILVVEAD
                     GVAVENMTARHYQINGFYWSAVQGYRGSWLTAYGNGDYGVYAYDSVWGRFDHSYASGH
                     PDAGFYVGECHPCHAVIDDVTAVHNSFGYSGTNAGGDLAVVNSEWTGNLAGIVPNTLD
                     SLLAPQRGALIAGNWVHGNAATDAPTKRLEYPAFGIGILIAGGSDNLVLGNLVTDNPT
                     YGIAMLPNLDDNLWATRNNEVRDNVALRSGRADLALGAPSIEGDCFAGNTFQTSLPPA
                     IQLLAGCDRRIAAVGEPAVTFGQLARNAQALGGHYPSGDWQVEPQPAAQPQMPDALTA
                     PAAPAVPETAVPGSYMVRTLDALRATADNAVTAGTGAAAREVMILGIPLASGLSTILG
                     LYGYVLPLVLYATWVAISLWDLVRREELTRRRRLGWMTVVLIVPFLGPPIYLLAGGSS
                     IDRAVRLFLVFGALAIYLVVAAAAFLVEAF"
     gene            complement(2899..3417)
                     /locus_tag="AC162A_05125"
     CDS             complement(2899..3417)
                     /locus_tag="AC162A_05125"
                     /inference="COORDINATES: protein motif:HMM:NF025698.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 family protein"
                     /protein_id="HIR5010256.1"
                     /translation="MRSQATGPRMVDARGARVGAAISVFLLVVAFDLHRSGSPERATI
                     LVAAVATAMAVSAATALRWNALSLPFQVARANGAVRPPTPDDLQPAVGLRIAQALGAT
                     FLVVALAAWALGVDGLAVLLIATLASLQALLAITGICLACRFYGLAVWFDRRRSPQGT
                     PPLATQRIRIQR"
     gene            complement(3427..>4266)
                     /locus_tag="AC162A_05130"
     CDS             complement(3427..>4266)
                     /locus_tag="AC162A_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR5010257.1"
                     /translation="SLAEQIRTNEAFHVDEDTFVANMLAKLRPYPRYFAFMGQRNAWF
                     PTSANLEPLPELALDELPDDSPAVTSRVILDVRPRSDYAAAHVEGSLHVDGRGNLASW
                     YGWVSSIEAEVTLVASGTEEAAAAQRELRRIGVDSIRGACIAPDLTAFAGAACGPVVS
                     MLRRATFADLAAELRLGEDIVVLDVRDEDERRALRIAGSLGIPVYDLPQADLEALRGR
                     NVWVHCAAGFRATLAASILERAGIRSAIVDDHIDAAADLGLAYEPIASVEREALAVAS
                     IAA"
BASE COUNT          604 a         1565 c         1538 g          559 t
ORIGIN      
        1 cgatcgagcc gcacacgccg agcccgacca ggcggccctc gaggcgccgg cggcccaccg
       61 tggcctcgcc gctcaccgcc ggcccgcctc catcgcggcc ccgcctgcga cctcggcgta
      121 gaggatcgcg aggcccttga gggtgagatc gggatcaatg gcgtccactc cctcgacgcc
      181 gcgtgcccag ggagccgccg agaggccgcc ggtcaggatg gcgcgcacgt cctcgggctt
      241 ggtgccggtc gcctccgcga gctccgcacg gacgcgggcg agcagccccg acgcgagcgc
      301 ctggtagccg aagatcgtgc ccgactgcat cgcgctgacg gtgtcgcggc cgattgcgcg
      361 gtccggcgtc cggagctcga tccgcggcag cttggcggtc cgtgacgcaa gcgcctccag
      421 gcccagctcg aggccaggtg cgatcgcgcc gcccacgtac gcgccgtcga ggccaacaca
      481 gtcgagcgtc gtcgcggtcc cgaagtcgac gaccaccgcg ggcgtgccgt acagccgcgc
      541 ggccgccagg gcgttgacca ggcggtccgc gccgacctca tggggtcggt ccacccggac
      601 gggaagcggc acgttcccgg ccgacgcgac gaggaggggg atcgcgcgcc gcgtcgcgac
      661 gatctcgatc gtggccgtga gcgacgggac gacggacgcg acggccagcc ccgagatgtc
      721 ggccagcgtg cgctcgtcca gcccgatcag gccggcgatc aggacgtcca gctcatctgc
      781 cgtcgcggac gccggcgtgc ccgcgcggcg cgtggacacg atcacgccgt cggcgacgag
      841 cgcgagggtg acgttggtgt tcccgatgtc gaccacgagc agcatgcgcg gatcatacgg
      901 gtgcgacccg acctgctagc ctcgcaccca atgcgactgc cgcgccccgg gcgctccctg
      961 tccccgttcg tgatcgcggc ggtcgtcgcg ctgccgctgg tcgccgcctg cacagcggtc
     1021 gtcctcgcca cttcgtacga cgagcgcgtc gtccgcaacc tcgacgactt cttcgatccc
     1081 caggtcgtgc ggatcgaccc cggcaccacg atcgagtggg tcaacgtcgg ccggaacccg
     1141 cacactgtca ccgcggacga cgggtcgttc gacagcggca ccctccagaa cgggcaggcg
     1201 tgggagcggc ggttcatgga gccgggcgtg ttcccgtact tctgccggct gcacggcgcc
     1261 cccgggatcg ggatgatcgg gaccatcgtc gtcggcgacg tgcagctccc cgacaaccgc
     1321 ggccggggcg gggtcagccc agggcgcgaa ccggttccga cgacgcccgg caccacgatc
     1381 cgcgtgcccg cggaggcgcc gacgatccag gagggcgtga accaggccaa tcccggcgac
     1441 ctcgtcctgg tcgcgcccgg cgtctacccc gagaccgtcc tcgtgcggac gccgttcctc
     1501 acgatccgcg gcgaggatcg caacgcggtg atcctcgacg gcgagttcaa gcgggcgaac
     1561 gggatcctcg tcgtggaggc ggacggggtc gccgtcgaga acatgaccgc ccggcactat
     1621 cagatcaacg gcttctactg gtccgccgtg cagggctatc gcggctcctg gctcacggcc
     1681 tacggcaacg gcgactacgg cgtgtacgcg tacgactcgg tctggggccg gttcgaccac
     1741 tcctacgcgt ccggccaccc cgacgcgggc ttctacgtcg gcgagtgcca tccctgccac
     1801 gcggtcatcg acgacgtcac cgcagtccac aactccttcg gctacagcgg caccaatgcg
     1861 ggcggcgatc tcgccgtggt gaactcggag tggacgggca acctcgccgg gatcgtgccc
     1921 aacacgctcg actcgctgct ggccccgcaa cgcggcgccc tgatcgcggg caactgggtc
     1981 cacggcaacg cggccaccga cgcgccgacc aagcgcctgg aatacccggc cttcggcatc
     2041 ggcatcctga tcgccggcgg cagcgacaac ctcgtgctgg gcaacctcgt caccgacaac
     2101 ccgacctacg ggatcgcgat gctgcccaac ctcgacgaca acctgtgggc gacgcgcaac
     2161 aacgaggtgc gcgacaacgt ggcgctgcgt tcgggacgcg ccgacctcgc gctcggcgcg
     2221 ccgtcgatcg agggggactg cttcgcgggc aacacgttcc agaccagcct gccgccggcc
     2281 atccagctcc tcgcgggctg tgaccgtcgg atcgccgcgg tcggggagcc cgcggtcacg
     2341 ttcggccagc tggcgcgcaa tgcgcaggcg ctgggcgggc actacccgtc gggcgactgg
     2401 caggtcgagc cgcagcccgc ggcccagccg cagatgccgg acgcgctgac cgcgccggcg
     2461 gctcccgccg ttcccgagac ggcggtgccg ggcagctaca tggtccggac gctcgacgcg
     2521 ctccgggcga ccgccgacaa cgccgtgacg gccgggaccg gcgcggcagc gagggaggtg
     2581 atgatcctgg gtattccgct ggcgtcgggg ctgtcgacga tcctcggcct gtacggctac
     2641 gtgctgccgc tcgtgctcta cgcgacctgg gtggcgatct cgctgtggga cctcgtgcgt
     2701 cgcgaggagc tcacgcgccg aaggcggctc ggctggatga cggtcgtcct gatcgtgccg
     2761 ttcctcggcc cgccgatcta cctgctcgcc ggcggttcgt cgatcgaccg ggcggtgcgg
     2821 ctgttcctcg tgttcggcgc gctcgcgatc tacctggtcg tggccgccgc cgcgttcctc
     2881 gtcgaggcgt tctagcgcct agcgctggat ccggatccgc tgggtcgcaa gcggcggggt
     2941 gccctgcggc gagcgccggc gatcgaacca gaccgccagc ccgtagaagc ggcaggcgag
     3001 gcagatgccg gtgatcgcga gcagcgcctg gagcgaggcg agggtggcga tgaggagcac
     3061 ggcgaggcca tccacgccga gcgcccacgc cgccagcgcg accacgagga acgtcgcccc
     3121 gagcgcctgc gcgatgcgca gccccaccgc gggctgcagg tcgtcgggcg tgggcggacg
     3181 gacggccccg ttcgcgcggg caacctggaa cggcagcgac agggcgttcc agcggagcgc
     3241 ggtcgcggcg ctcaccgcca tggccgtggc gaccgcggcg acgaggatcg tggcccgttc
     3301 gggtgagccg ctgcgatgga ggtcgaaggc cacgaccagg aggaacacgc tgatcgcggc
     3361 gccgacgcgc gccccgcgcg cgtcgaccat ccgggggccg gttgcctggc tgcgcatcac
     3421 ccgcccctat gccgcgatcg acgcgacggc cagggcctcg cgctcgaccg aagcgatcgg
     3481 ctcgtaggcg agaccgaggt ccgcggcggc gtcgatgtgg tcgtccacga tcgcgctccg
     3541 gatccccgcc cgctccagga tcgacgcggc cagcgtcgcc cggaagcccg ccgcgcagtg
     3601 gacccacacg ttccgcccgc gcagcgcctc gaggtcggcc tggggcaggt cgtagaccgg
     3661 gatcccgagc gagcccgcga tccgcagggc tcggcgctcg tcctcgtcgc ggacgtcgag
     3721 cacgacgatg tcctccccca gccgcagctc cgcggcgagg tcggcgaagg tcgcccggcg
     3781 aagcatcgaa accaccggac cgcacgcggc acccgcgaac gccgtgaggt cgggggcgat
     3841 gcaggcgccc cggatcgagt cgacgccgat ccgccgcagc tcgcgctggg cggcggccgc
     3901 ctcctcagtg ccggaggcca ccagggtgac ctcggcctcg atcgaggaca cccagccgta
     3961 ccagctcgcg aggttgccgc ggccgtcgac gtgcaggctc ccctcgacgt gggcggccgc
     4021 gtagtcggac cgtgggcgga cgtcgaggat cacccgagag gtgacggccg ggctgtcgtc
     4081 gggcagctcg tcgagcgcca gctcgggcag cggctcgagg ttggccgacg tcgggaacca
     4141 ggcgttgcgc tggcccatga acgcgaagta gcgcgggtag ggccgaagct tcgcgagcat
     4201 gttcgcgacg aacgtgtcct cgtcgacgtg gaacgcctcg ttggtccgga tctgctccgc
     4261 gagcga
//
LOCUS       DBNHXO010000248         7570 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1852005, whole genome shotgun sequence.
ACCESSION   DBNHXO010000248 DBNHXO010000000
VERSION     DBNHXO010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7570)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7570
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1852005"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="AC162A_05135"
     CDS             <1..714
                     /locus_tag="AC162A_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010258.1"
                     /translation="IYVATNNGVNSEDIGWLAGQAGVTTRTSTIDGDGFEGTPVVAAS
                     GANVLVAWLDRDDGTIKARISTDHGHTWPDPAIDLATGQAYGMSAAADASRLALAWAA
                     PDGIRSRVFRDDTWKATHKLASFSSTGAYRNGYGTSVALAGSSRLGVAWSACTRANCA
                     GSSAQGVNVRWRESANNGSSWKPSVTIASHGAGSDRRINDYPSALMLDSSHRLVTYNT
                     AARNFSTYRLVIERGAGKP"
     gene            complement(721..1488)
                     /locus_tag="AC162A_05140"
     CDS             complement(721..1488)
                     /locus_tag="AC162A_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5010259.1"
                     /translation="MSRATATGGGRSVILTIAALTWRGLLGRRRSLLLTLLALLPVAI
                     AILVRLSRAGTSEEVTTAIMDRLIVTTMLPIVALVFGTAAIGAEIEDGTAVYLLVKPI
                     DRWRIVVAKLAVAIGLSVALVAPASFVAGAILQPAGTGLSGAVGAAVGTAIGATVYVT
                     IFFALSLVTGRSLAIGLIYILVWEGVLAGLLEGTRLLSIRQYTLAISDAIAHPGVTNP
                     NLLDAWTALALAVVAVAFAAAIAVRRLAVFEIGQADD"
     gene            complement(1485..2483)
                     /locus_tag="AC162A_05145"
     CDS             complement(1485..2483)
                     /locus_tag="AC162A_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014687889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5010260.1"
                     /translation="MSPRSDAPLWGPAATATPGRAPGPRTLTPGAAVELLGVSRWYGN
                     VVAVNDISFVLGSGVTGLLGPNGAGKSTILHMIAGLLRPSDGVVAMRGTSSWRNPAVY
                     REMGLVPERESVHPFLTGFEFAQLNARLQRLPDPDGAARTAIATVGLTDAADRAVGTY
                     SKGMRQRAKIAGALVHDPSILVLDEPFNGMDPRQRLQMMELLARMADQGRVILFSSHI
                     LEEVERLAQNVLVIVSGRLAASGDFREIRRLMTDRPHTFTMRSSDDRRLAAALVGHPS
                     TFGVQLDQGWLAVRTSDYVGFARALPAIARASGVSIIELRPTDEDLESVFGYLVQR"
     gene            complement(2480..3376)
                     /locus_tag="AC162A_05150"
     CDS             complement(2480..3376)
                     /locus_tag="AC162A_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010261.1"
                     /translation="MSAGPSGSIYDIGYRGYDGPRLGRRHAFATLFTGGLRATFGLGR
                     SARAKIVPAFCIVVPAVTALIIVALRTFASRFGITDLGVIPGHAEMASSVGVFATILV
                     AVQAPELLGRDVRYRVLSLYFSRALLRVDYALAKLAALCAALLILFLVPHIILTVGAV
                     LLTTDVVDALGHEAGAWLAILGSAVLAAVATSGVALVAAAFVARRAYATVTIFGLFIV
                     PGIIAAVVVALDLGEASRYVVLLDIGAVLDAANAWFFGVDPSSGTWAATGLPVSLGLV
                     ASIVIAIGSGTILVQRYRTIAA"
     gene            complement(3373..4344)
                     /locus_tag="AC162A_05155"
     CDS             complement(3373..4344)
                     /locus_tag="AC162A_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006141355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5010262.1"
                     /translation="MPEAAAGIPVPLIRVEALTKRFPGVVALDELTVDVAPGIVGLVG
                     ANGAGKSTLIKILLGLLAPTSGRVEVMGYDPTTQGTRLRRYVGYMPEHPALPPDVSAT
                     EFVAQAGRLSGLPTKVARERTADVLRHVGLAEERYRPIAGYSTGMRQRVQLAQAIVHD
                     PRLLLLDEPTNGLDPAGRDEMLGLIRRTGTEFGIAIVLASHLLGEIEAVCDHLVAIDG
                     GHLLRAAPMAAFTAETGILTVEVEEGRDTLAAGLADRGLRVLEGSGGADRTVRIALDG
                     GAPFDIVRDTVADLGLPLVRMEHGRHSLEDLFRDDVPAASATTGASA"
     gene            4899..4973
                     /locus_tag="AC162A_05160"
     tRNA            4899..4973
                     /locus_tag="AC162A_05160"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4931..4933,aa:Lys,seq:ttt)
     gene            complement(4995..6605)
                     /locus_tag="AC162A_05165"
     CDS             complement(4995..6605)
                     /locus_tag="AC162A_05165"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR5010263.1"
                     /translation="MTAVRTPPQPSRLLTLGGTTYPVFLPSIRDPRLHVAAVIITIHV
                     LGQVGLHFRVSVPQILAAIITTAVIEVVLTFRQTKSFVWPASAMLTGSGVALILRVVG
                     TPPDDPWNTYAWYVFAGVAAFSLLTKYVIKYRGSHVFNPSNIGLVIAFIVLGSTRIEP
                     LDFWWAPITNPLMILAYTVIIGGGLLITRRLRLLALAGTFWVTLAIGVGLLAATGHSM
                     VANWAFAPVGGFDYWRVIVFSPEVMIFLFFMITDPKTVPAGRVGRVIFGFLVAVMSTL
                     LMAPQVDEFGTKVGLLAGLVVVCAARPILDRLLPAPKSTSDEIGLFARRVALGGNAGL
                     VRGVVGVGLTLGLVLVLGVGIVAAGSTTRGVARVNTEGILDRVPHEIDPATFPSITVG
                     KDVSDWNHAIAGDAARDIVLTMAENLELENQALLRGDAGILDAVDHGDRLDEMQARLH
                     EASATGTTTIDRYRFDAIDMSLITPFGKQDGLSLGLNATGTMTEETYDDAGTLLSSHD
                     SPFARIFVVRRATGDRWLNVGVLPPAGS"
     gene            complement(6670..7452)
                     /locus_tag="AC162A_05170"
     CDS             complement(6670..7452)
                     /locus_tag="AC162A_05170"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019748662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase family protein"
                     /protein_id="HIR5010264.1"
                     /translation="MTDAPLPSLYLGHGAPPLLDDPQWMAELSGWAGDLPRPKSILIV
                     SAHWQTAPMAIGATTTVPLVYDFYGFPQRYYEYEYRSPGAPELAATVKSLMPANEPIL
                     DRPGRGLDHGAWVPLLAMYPDADIPVLQMSMPDLDPAHLFEVGKRLAPLRDEGVLIVG
                     SGFMTHGLPYIGEYFAGKPGAPQWSVEFDRWVAEALERGDLDTLFAFREKAPGMPYAH
                     PTVEHFAPLFVTLGAAAQPDDTPKTAIDGFFIGLSKRSIEAR"
BASE COUNT         1248 a         2582 c         2737 g         1003 t
ORIGIN      
        1 atctacgtgg cgaccaacaa cggggtgaac agcgaagaca tcgggtggct ggccgggcag
       61 gccggtgtta ccacgcgaac gagcaccatc gacggcgacg gcttcgaggg cacgccggtc
      121 gtcgcggcct cgggcgcgaa cgtcctcgtc gcctggctgg accgcgacga cgggacgatc
      181 aaggccagga tctcgaccga ccacggtcac acctggcccg atccggccat cgacctcgcg
      241 acgggccagg cgtacggcat gtctgcggcc gccgacgcct cacgcctcgc gctcgcctgg
      301 gcagcgccgg acggcatccg gtcgcgggtg ttccgggacg acacgtggaa ggcgacgcac
      361 aagctcgcct cgttcagctc gacgggcgcc taccgcaacg gctacggcac ctcggtcgcc
      421 ctggccgggt ccagccggct gggtgtcgcc tggtccgcgt gcacgcgcgc gaattgcgcc
      481 ggctcctccg cccagggcgt caacgtccgg tggcgggagt cggcgaacaa cggcagcagc
      541 tggaagccgt cggtgacgat cgcatcgcac ggcgccggca gcgaccggcg catcaacgac
      601 tacccgtcgg cgctgatgct cgactcgagc caccgcctcg tcacctacaa caccgcggcc
      661 cgcaacttct cgacctaccg gctggtgatc gagcgaggcg ccggcaagcc gtagggcgta
      721 tcagtcgtcg gcctggccga tctcgaacac ggccaaccga cgcaccgcga tggccgccgc
      781 aaaggcgacg gccacgaccg cgagggcgag cgccgtccat gcgtcgagga gattcgggtt
      841 ggtcacgccg ggatgtgcga tcgcgtctga gatcgcgagc gtgtactggc gaatgctcag
      901 caaccgggtc ccctcgagca gcccggccag aacgccctcc cagacgagga tgtagatgag
      961 cccgatcgcg agtgaccgcc ccgtgacgag gctgagcgcg aagaagatgg tcacgtagac
     1021 ggtggccccg atcgcggtcc ccaccgccgc ccccaccgcc ccggagagcc cggtgccagc
     1081 cggctgaagg atggcaccgg caacgaacga cgccggcgcc acgagcgcga ccgagaggcc
     1141 gatggccacg gccagcttcg cgaccacgat ccgccaccgg tcgatcggct tgacgaggag
     1201 gtagaccgcg gtcccgtcct cgatctccgc gccgatggcc gccgtgccga agacgagggc
     1261 gacgatcggc agcatcgtgg tgacgatgag gcggtccatg atcgcggtcg tgacctcctc
     1321 actcgttccg gccctcgaca ggcggacgag gatcgcgatc gcgaccggca gcagggccag
     1381 cagcgtcagc agcaggctgc gtcgccggcc gagcaagcca cgccacgtca gggccgcgat
     1441 cgtgaggatg acggagcgcc caccgcctgt cgcggtcgcg cgactcaccg ctgcaccagg
     1501 tagccgaaga cgctctcgag gtcttcgtcg gttggacgga gctcaatgat cgacacgccg
     1561 ctcgcgcggg caatcgccgg cagcgcccgc gcgaagccga catagtcgga cgtccgtacg
     1621 gcgagccagc cctggtcgag ctggacgcca aaggtcgagg gatgaccgac gagggccgcc
     1681 gccaggcgcc ggtcgtcgga cgagcgcatc gtgaaggtgt gcggccggtc ggtcatgagc
     1741 cgccggatct cgcggaagtc gcccgacgcg gcgagtcgcc cactgacgat cacgagcacg
     1801 ttctgcgcga gtcgctcgac ctcctcgagg atgtgggagc tgaacaggat gacccggccc
     1861 tggtcggcca tccgcgcgag cagctccatc atctggagtc gctggcgggg atccatcccg
     1921 ttgaacggct cgtcgaggac caggatcgag gggtcgtgca cgagggcccc ggcgatcttg
     1981 gcccgctggc gcatcccctt ggagtaggtc cctaccgcac ggtccgcggc gtccgtgagg
     2041 ccgaccgtgg cgatcgcggt gcgggcggcc ccatccgggt ctgggaggcg ctggagccgc
     2101 gcgttgagct gggcgaactc gaacccggtc aggaacgggt ggaccgactc gcgctccggg
     2161 acaaggccca tctcgcggta gaccgccggg ttccgccagc tcgaggtgcc ccgcatcgcg
     2221 acgacgccgt cggagggccg gagcagcccg gcgatcatat ggaggatcgt ggacttgccg
     2281 gctccgttcg gcccgagcag cccggtcacc cccgatccca ggacgaacga gatgtcgttg
     2341 acggcgacga cgttgccata ccagcgcgag acgccaagga gctcgacggc cgcgcccggc
     2401 gtgagcgtgc ggggaccggg cgcccgaccg ggagtggcgg tggcggccgg accccagagc
     2461 ggagcgtccg agcgcgggct catgctgcga tcgtccggta gcgctggacg aggatcgtgc
     2521 ccgacccgat cgcaatcacg atggaggcga ccaggcccag cgacaccggc agtcccgtgg
     2581 ccgcccaggt cccggacgac gggtcgacgc cgaagaacca ggcattcgcg gcatcgagga
     2641 ccgcgccgat atcgaggagc acgacatacc gcgaggcctc gccgagatcg agtgcgacga
     2701 ccacggcggc gatgatcccg ggcacgatga acaggccgaa gatcgtcacg gtcgcatacg
     2761 cccgccgggc aacgaacgcc gcggccacga gcgccacgcc ggacgttgcg accgccgcga
     2821 ggacggcgga accgaggatc gcaagccagg ccccggcctc gtggccgagt gcgtcgacca
     2881 cgtcggtcgt gagcagcacg gcccccactg tcaggatgat gtggggcacg aggaacagga
     2941 tgagcagggc cgcgcagagc gccgccagct tcgccagcgc atagtccacg cggaggaggg
     3001 ctctggaaaa gtacaggctg aggacccggt accggacgtc gcggccgagc agctcgggcg
     3061 cctgcaccgc gacgaggatc gtggcgaaca cgccgaccga gctggccatc tccgcatgac
     3121 ccggaatcac cccgagatcc gtgatgccga accgcgaggc gaacgtgcgt aacgccacga
     3181 tgatcagggc ggtgacggcc gggaccacaa tgcagaacgc cggcacgatc ttcgcgcggg
     3241 cggaccgacc gagcccgaac gtcgcccgaa gacctccggt gaacagcgtg gcgaacgcgt
     3301 gacgacggcc cagcctcggc ccgtcatagc cgcggtagcc gatgtcgtag atgctgcccg
     3361 acggcccggc gctcacgcgc tcgctcccgt cgttgccgag gccgcgggca cgtcgtcgcg
     3421 gaacaggtcc tcgagcgagt ggcggccgtg ctccatgcgg accaggggga ggcccaggtc
     3481 ggccacggtg tcgcggacga tgtcgaacgg cgccccgccg tcgagcgcga tccgcaccgt
     3541 ccggtccgcg ccgcccgagc cctcgagaac gcgcaggccg cggtcggcca gaccagccgc
     3601 gagggtatcg cgcccttcct cgacctccac cgtcaggatc ccggtctcgg ccgtgaacgc
     3661 ggccatcggg gccgcccgaa gcaggtgccc gccgtcgatc gccacgagat ggtcgcagac
     3721 cgcctcgatc tcgccgagga gatggctggc aagcacgatg gcgatcccga actcggtccc
     3781 ggtccgacgg atgaggccga gcatctcgtc gcgacccgcc ggatcgagcc cgttcgtggg
     3841 ctcgtcgagc aggagcagac gcgggtcgtg gacgatggcc tgggcgagct ggacgcgctg
     3901 gcgcatcccg gtggaatagc ccgcgatcgg ccggtaccgc tcctccgcga gccccacgtg
     3961 acggaggacg tccgccgtcc gttcacgagc gaccttggtc gggagacccg agagccggcc
     4021 ggcctgggct acgaactccg tcgcgctgac gtccggcgga agcgcgggat gctcgggcat
     4081 gtagccgaca taccgccgga gccgcgtgcc ctgggtcgtg gggtcgtagc ccatgacctc
     4141 gacgcgaccg ctcgtcggag cgaggaggcc gagcaggatc ttgatcaacg tgctcttgcc
     4201 ggccccgttg gcaccgacca ggccgacgat cccgggcgcg acatcgaccg tcagctcgtc
     4261 gagggcgacc acacccggaa accgcttggt cagcgcttcg acgcggatca acggcaccgg
     4321 gatcccggct gcggcctcag gcatgagccc gtgtgggatt cgtggccgcg atcgctgcga
     4381 ggcgcgctct caccagggcg tgcgcaccct cgagacgacg ctcgaggggc tcgcgccgct
     4441 ggccgtgtgg acgggatcgg tgtcggtcct gatgccccga agtatgaggc acgtcccgat
     4501 gaccagctcg aacggcagga tcggccttag gcggcgattg ggacgcgatt cctctcgcgc
     4561 gacgctcgcg ttccgaggac ccgttacctc gatctggcga cgtgcgacgc gcgaacgcgt
     4621 aggacatctc ggctggatcc ttgctgctat gcgactgccg gtcccggggg ccgcgggccg
     4681 cgttccgtgc gcgttcttca ggcccgacgc agcgtccgtc gcaggatgac aagatcgctg
     4741 cccttcgtcc ggcgcccatt cgataatcgc cacggcgagc gagtcccgcc agagccaacc
     4801 ggctcgaact ccgctgccga ggcgagcggc ggtctagtag cctcaacgct ccgggtcacg
     4861 tgccgccgat gcggtccgcg gatctggcga caaaccatgg gcccgtagct cagcggtaga
     4921 gcaggggact tttaatccct tggtcctggg ttcgaatccc agcgggctca ccagctcccg
     4981 cgtcggcgcc tcgctcagct ccccgcgggc ggcagtactc cgacgttgag ccagcgatcg
     5041 ccggtggcgc gacgcaccac gaagatccgg gcgaacggcg aatcgtggct ggacaggagc
     5101 gtgccggcgt cgtcgtaggt ctcctcggtc atcgtgccgg tggcgttcag gccgaggctg
     5161 aggccgtcct gcttgccgaa cggcgtgatc agcgacatgt cgatggcgtc gaaccggtag
     5221 cggtcgatcg tcgtcgtgcc cgtcgccgag gcctcgtgga ggcgggcctg catctcgtcc
     5281 aggcgatccc cgtggtccac ggcgtcgagg atgccggcgt cgccccggag cagtgcctgg
     5341 ttctcgagct cgaggttctc cgccatcgtc agcacgatgt cgcgggcggc gtcgccggcg
     5401 atggcgtggt tccagtcgga gacgtccttg cccacggtga tcgacgggaa cgtggccggg
     5461 tcgatctcgt gcggcacgcg gtcaaggatg ccctccgtgt tcacccgggc gaccccgcgt
     5521 gtggtgctgc cggcggcgac gatgccgacc ccgagcacca ggacgaggcc aagcgtcagc
     5581 ccgacgccga cgacgccgcg gaccaggccg gcgttcccgc cgagcgcgac ccggcgcgcg
     5641 aacaggccga tctcgtccga ggtcgacttc ggcgccggca gcaggcggtc gaggatcggg
     5701 cgagcggcgc acacgacgac gagaccggcg agcaggccga ccttcgtacc gaactcgtcg
     5761 acctggggcg ccatcagcag ggtgctcatc acggccacga ggaacccgaa gatcacccgc
     5821 ccgacgcgcc ccgccggcac ggtcttgggg tccgtgatca tgaagaacag gaagatcatg
     5881 acctcgggcg agaacacgat gacccgccag tagtcgaacc cgccgaccgg ggcgaacgcc
     5941 cagttcgcga ccatcgagtg cccggtggcg gccaggagac cgacgccgat cgcgagcgtg
     6001 acccagaacg tccctgcgag cgcgagcagg cgcagccggc gcgtgatcag cagtccgccg
     6061 ccgatgatga cggtgtaggc gaggatcatc agcggattgg tgatcggcgc ccaccagaag
     6121 tcgagcggct cgatccgcgt gctgccgagc acgatgaacg cgatcacgag gccgatgttc
     6181 gacgggttga acacgtgcga gccgcggtac ttgatgacgt acttggtgag cagcgagaac
     6241 gccgcgacgc ccgcgaacac gtaccaggcg taggtgttcc agggatcgtc gggcggggtc
     6301 ccgaccacgc gcaggatcag cgcgacaccg ctccccgtga gcatcgccga ggccggccac
     6361 acgaacgact tcgtctggcg gaaggtcagg acgacctcga tgacggccgt ggtgatgatc
     6421 gccgcgagga tctgcggcac gctcacccgg aagtggagcc cgacctggcc gaggacgtgg
     6481 atcgtgatga tcacggccgc gacgtgcagg cgcgggtcgc ggatgctggg caggaacacc
     6541 gggtatgtcg tcccgcccag cgtgagcagg cgcgagggct gcgggggagt ccgaacggct
     6601 gtcatcgatc ctcaccgggg gaaacggcgg taagcatgcc tgacgaggcc gcccgacgca
     6661 gggtgagggt cagcgcgcct cgatcgagcg cttcgagagg ccgatgaaga agccgtcgat
     6721 ggccgtcttc ggcgtgtcgt cgggttgcgc ggccgcgccg agcgtcacga acagcggcgc
     6781 gaagtgctcg acggtcgggt gggcgtaggg catgccgggc gccttctcgc ggaacgcgaa
     6841 cagcgtgtcg aggtccccgc gctcgagggc ctccgcgacc cagcggtcga actcgaccga
     6901 ccactgcggc gcccccggct tgccggcgaa gtactcgccg atgtacggca ggccgtgggt
     6961 catgaacccg gatccgacga tcaggacgcc ctcgtcgcgg agcggcgcga gccgcttgcc
     7021 gacctcgaac aggtgggccg ggtcgaggtc gggcatcgac atctggagga ccgggatgtc
     7081 cgcgtcggga tacatcgcga gcagcggcac ccaggcgccg tggtcgaggc cgcggcccgg
     7141 gcggtcgagg atgggctcgt tggccggcat cagcgacttg acggtcgccg cgagctccgg
     7201 cgcgccgggg gagcggtact cgtactcgta gtagcgctgc gggaagccat agaagtcgta
     7261 gaccagcggg accgtggtcg tggcgccgat cgccatgggc gcggtctgcc agtgggcgct
     7321 gacgatgagg atggacttgg gtctgggcag gtcgccggcc cagcccgaga gctccgccat
     7381 ccactgcggg tcgtcgagta gcggcggcgc accgtggccg aggtagagcg agggcagggg
     7441 agcatcggtc atgtcgaggg ctcactcatc gagggaacgg taagcgcggc gatgcgtccc
     7501 acgcatcgtt tctggtcggt gatgtcgccg ggttgcgcgg cgcgcatcag tccgacctga
     7561 tggagccccg
//
LOCUS       DBNHXO010000249         1820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5226142, whole genome shotgun sequence.
ACCESSION   DBNHXO010000249 DBNHXO010000000
VERSION     DBNHXO010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5226142"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="AC162A_05175"
     CDS             <1..896
                     /locus_tag="AC162A_05175"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4 family protein"
                     /protein_id="HIR5010265.1"
                     /translation="SGPDYPMLRDALEKLHLNDASFTYEPESSVALGFGFRCGFLGLL
                     HMEIVQERLEREFNLDLIASAPSVEYRVGLTMNRGVVTVDNPARFPDVGEIDVIEEPW
                     VNLGVVTPDRYIGSLMELTTGRRGTFVNMEYLDPQRVHMHFELPLAELIVDYYDQLKS
                     RTQGYASMDYEETGYRAANLVKLDILVNNEPVDALSLIVHRDRAQLVGRQLVEKLRQL
                     IPRQMFEVPVQAAIGSHVVARETIRAMRKNVLAKCYGGDITRKRKLLEKQKEGKQRMK
                     RVGSVEIPQEAFLAILRMDEA"
     gene            932..1732
                     /locus_tag="AC162A_05180"
     CDS             932..1732
                     /locus_tag="AC162A_05180"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR5010266.1"
                     /translation="MPDVDLAGLPGISDIPDVLRVLMAVGLAGLLVLLRFDAERFSAA
                     EYDDVDRWGNKPSLRRRLGWYLLGIGGILAILWIHPNPANDLFLGLGDRLSAVMLGLA
                     YGAIGTAIAIGIALYRYQTVRLPHMTSYPGAIINAILTAFLDEAIFRGLLFGFLLMTG
                     MNPNLANLIQALIYALATRLGAPGRPWYMLVTALVMGLAGGWLVGITGGIGAAFLGHA
                     ITRVAIFLTTGHAGLPKARGTEVEEVARRQDLPKGWHAVGSGEATRDK"
BASE COUNT          283 a          649 c          590 g          298 t
ORIGIN      
        1 tcagcggacc cgactacccg atgctccgcg acgccctcga gaagctccac ctcaacgacg
       61 cgagcttcac ctacgagccc gagtcgtcgg tcgcgctcgg gttcgggttc cggtgcggct
      121 tcctcgggct cctccacatg gagatcgtcc aggagcgcct cgagcgcgag ttcaacctcg
      181 acctgatcgc gagcgcgccg tcggtcgagt atcgggttgg actcacgatg aaccggggcg
      241 tcgtcacggt cgacaacccc gcacgtttcc cggacgtggg cgagatcgac gtgatcgagg
      301 agccgtgggt caacctgggc gtcgtgacgc ccgaccgcta catcgggtcg ctgatggagc
      361 tcacgaccgg ccgccgcggc acgttcgtga acatggagta cctcgatccc cagcgggtcc
      421 acatgcactt cgagctgccg ctcgcggagc tgatcgtcga ctactacgac cagctcaaga
      481 gccgcacgca gggctacgcg tcgatggact acgaggagac cggctatcgc gcggcgaacc
      541 tcgtcaagct cgacatcctc gtcaacaacg agcccgtcga cgccctcagc ctcatcgtcc
      601 atcgcgaccg ggcccagttg gtcggccgtc agctggtcga gaagctgcgc caactgatcc
      661 cgcgccagat gttcgaggtc ccggtccagg cggccatcgg cagccacgtc gtcgcccgcg
      721 aaacgatcag ggcgatgcgc aagaacgtac tggcgaaatg ctacggtggc gatatcaccc
      781 gcaagcgcaa gctgctcgag aaacagaaag agggcaagca gcgtatgaag cgcgtgggat
      841 cggtcgagat cccgcaggag gcatttctgg cgatcctgcg catggacgag gcgtgatgcg
      901 ccgcgtcctg tctcgctgag cccggccgcc ggtgcctgac gtggatctcg cgggcctgcc
      961 cgggatctcg gacatccccg acgtgctgcg cgtcctgatg gctgtggggc tggccggcct
     1021 gctcgtgctc ctccgcttcg atgccgagcg cttcagcgcg gccgagtacg acgatgtcga
     1081 ccgctggggg aacaagccct cgctccgccg gcgcctcggc tggtacctgc tcggcatcgg
     1141 gggcatcctc gcgatcctgt ggatccaccc caaccctgcg aacgacctgt tcctcgggct
     1201 gggcgaccgc ctgagcgcgg tgatgctggg tctcgcgtac ggcgcgatcg gcacggctat
     1261 cgccatcggc atcgcgctct accgctatca gacggtccgc ctgccccaca tgacgtcgta
     1321 ccccggcgcg atcatcaacg cgatcctgac cgcgtttctc gacgaggcca tcttccgcgg
     1381 gttgctgttc ggcttcctgc tcatgaccgg catgaacccc aacctcgcca acctgatcca
     1441 ggcgctgatc tacgcgctcg cgacgcggct cggggcaccg ggccggccgt ggtacatgct
     1501 cgtcaccgcg ctcgtgatgg gcctggccgg cggctggctg gtcgggatca ccggcggcat
     1561 cggcgccgcg ttcctgggcc atgcgatcac ccgggtcgcg atcttcctca ccaccggcca
     1621 cgccgggctg cccaaggccc gtggaaccga ggtcgaggag gtcgcccgcc gccaggacct
     1681 ccctaagggc tggcacgcgg tcgggtccgg cgaggcgacc agggacaagt agcgacgtca
     1741 gccccgggct cggcgtcccc gaccatcgcc ccgcggccgc cggtcgcgct ctacgtccac
     1801 gtgccgttct gcgtctcact
//
LOCUS       DBNHXO010000250         4629 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3043624, whole genome shotgun sequence.
ACCESSION   DBNHXO010000250 DBNHXO010000000
VERSION     DBNHXO010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3043624"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..>4629)
                     /locus_tag="AC162A_05185"
     CDS             complement(250..>4629)
                     /locus_tag="AC162A_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5010267.1"
                     /translation="AFLYLLNPDTGILHFAHSAGIVDPEPGHWIRELRLPIGTGMFGK
                     AVAERRMVVTRDYVEDRSFSHATGPDRFVDEVGLRSLVVAPLVAGMEVFGALGTFSRR
                     PDAFSAPQIALVRSLAEHAAFAMANARLITELDRSRQAGERQAVVERSLRELGTRISG
                     ARDPESVVQFTIDEALRLLNGDGARIDIVDPEARLLRGLLASGEEQILETEWPKDPDD
                     SLEVGASGKAVVSGETYISRDYISDPNLVHGKGPDTYVRTKGIHGVIATPLHGDQGPF
                     GAITVWSTKVDAFGPDDAALLETIAGQSAVALGRARLIEELGRSREALARRAEEERTL
                     REIGGRLATIGSDPGDLLLRIVHEAARLLGAERARLDLIEPVKGAVLWTYPEGEPFTD
                     PPVLDAPPDEEPIGLAGLAVREGRPVASGDYLNDRRFRHYPEGDEGVRAIGALSVVTA
                     PVVGEDGLMGVFQAVHREKDAFGDDELRLLDAMAAQAAVALANARLVDRLASSQRALE
                     RTADAERALREIARRMMTIQDPAELLQDVVDEAARLLGSSGAVIDLLDPATGEVRWAH
                     DAGIDDATRTEWQSLGVGGSGVHLAIRERQVLVTDDYGADERFADGPPNREFFERVGI
                     RSIAFAPLVGEAVVLGTLAVFSGDTGQFGDQEGALLAALADLATIAIHNAELIRELGR
                     SREETARRAETERTLREIAARVTSIRDADAILGLIVEETRRVLASDGAHLTRMADDRT
                     YLTPMVVAGGMDDRARDWLRSQQFPINGGINGLAAGQGRVVWTPSYATDPRIPREADD
                     LDLAERMGLGAMAAAPLRAPGGDVIGTLAVSYRQPGSIAPDRLATLQALADHAAIALS
                     NSDLLERVEASEANYRGLVQTTPDVIWRNDADGLFTFLADNVEGLFGYTPVEMTGQHF
                     SFVMAAESMDDAGQAWADLHDPERTSLRLRFVVRRKDGSQFPTEVSAVGIFEDGVFVG
                     AQGTIRDTSERERLELELRSSEERYRFLVENAPDIVFSADQATRFIFISDTVERLTGW
                     RPDELIGETFERLVTPASLPIAADRWERIAADPTQPQVLRLELVRRDGGTVPVEIHSV
                     AQLDGNGDFAGVHGSARDIGERERLERELRDSEVRYRYLVQSSPDLVWMTDDVGRLTF
                     VSDQSGSILGWEPEELIGRSFSELAPPEERRGSLARFRALRKRPHEVHRTRLKIQSRD
                     GRDLAMEITSIGMVQDGEFIGAHGAARDVSERDRLERDHRRQAVELASSEERSHLARE
                     LHDSVTQALFSMTLLSRSIEILLVKDPDQVPGKLASLRELQREALAEMRALIFELRPG
                     NVEENGLIQALRTHSAALSGRIGLPVVVEGELADRPSLDCEEGLYRIAQEALHNVVKH
                     AGAKQVRVEVGRVPDGVHLRVIDDGRGFDPSAVPDGHLGLTGMQARAERLGGTITVSS
                     VRDGGTTIDVVVPDGRAVEATPA"
BASE COUNT          631 a         1643 c         1707 g          648 t
ORIGIN      
        1 cgacgacggt gaagcggtgt cgaatgccga ggatcccgac catgctctcg cgcgtgcggt
       61 catcggcgtc gacgaccagc aggcgggtgc cgccggcagc cagtgacgtg gctgcaacgg
      121 cggaggcgag gacgtggtcg gacatgagac cgatcctgcc cgacgatctc catgccggtc
      181 agggacgcgc ggcggagatg acaatccggc aagtgacgca gccggatgcg acattcgtcg
      241 cgcaggcgct caggccgggg tcgcctcaac agcgcgacca tccggcacga ccacgtcgat
      301 cgtggtgcca ccgtcgcgca ccgatgagac ggtgatcgtc ccgcccaggc gctccgcgcg
      361 cgcctgcatt ccggtcaaac cgaggtgacc gtccgggacg gcggagggat cgaacccgcg
      421 gccgtcgtcg atgacgcgga ggtgcacgcc gtcgggcacc cgcccgacct cgaccctgac
      481 ctgcttcgcg cccgcgtgct tgacgacgtt gtgcagcgcc tcctgggcga tgcggtacag
      541 cccctcctcg caatcgagcg acggccggtc ggcgagctcc ccctccacca cgaccggcag
      601 gccgatccgt cccgacaggg cggcgctgtg cgtgcgcagg gcctggatga gcccgttctc
      661 ctcgacgttg ccgggtcgca gctcgaagat cagcgcccgc atctcggcca gcgcctcacg
      721 ctggagctcg cgcagcgacg cgagcttgcc gggcacctgg tcaggatcct tgaccagcag
      781 gatctcgatg ctccgcgaga gcaatgtcat cgagaacagc gcctgggtca ccgagtcgtg
      841 gagctcgcgg gcgaggtgtg agcgctcctc ggacgacgcc agctcgacgg cctggcggcg
      901 gtggtcgcgc tcgaggcgat cgcgctcgct gacgtcgcgg gccgcgccgt gggcgccgat
      961 gaactcgccg tcctggacca tcccgatgct cgtgatctcc atggcgaggt cgcgcccgtc
     1021 ccggctctgg atcttgagcc gcgtccggtg cacctcgtgc gggcgcttcc gcagggcccg
     1081 gaagcgggcc agggagccgc ggcgctcctc gggcggggcg agctcgctga acgagcggcc
     1141 gatgagctcc tcgggctccc agccgaggat cgaccccgac tggtcggaga cgaacgtgag
     1201 gcggccgacg tcgtccgtca tccacacgag gtcgggcgac gactggacga ggtagcggta
     1261 gcggacctcg gagtcgcgca gctcgcgctc caggcgctcg cgctcgccga tgtcgcgcgc
     1321 ggagccgtgg acgcccgcga agtcgccgtt gccgtcgagc tgggcgacgg aatggatctc
     1381 gaccgggacc gtgccgccat ccctgcggac cagctccagc cgcagcacct gcggctgggt
     1441 cgggtcggca gcgatgcgct cccagcggtc ggccgcgatc ggcagcgacg cgggcgtgac
     1501 gaggcgctcg aacgtctcgc cgatcagctc gtccggccgc cagcccgtca ggcgctcgac
     1561 cgtgtcggag atgaagatga agcgggtcgc ctggtccgcg ctgaatacga tgtcgggcgc
     1621 gttctcgacc aggaaccggt agcgctcctc ggacgaccgg agctcgagct ccaggcgctc
     1681 gcgctcgctg gtgtcccgga tcgtgccctg ggcgccgacg aagactccgt cctcgaagat
     1741 cccgacggcc gacacctcgg tcgggaactg gctgccgtcc ttgcggcgga cgacgaaccg
     1801 caggcgcagg gacgtgcgct ccgggtcatg gaggtcggcc cacgcctgcc cggcgtcgtc
     1861 catcgattcg gcggccatca cgaacgagaa gtgctggccg gtcatctcga cgggtgtgta
     1921 gccgaacagc ccctcgacgt tgtccgcgag gaacgtgaac aggccgtcgg catcgttgcg
     1981 ccagatcacg tccggcgtcg tctgcaccag gccgcggtag tttgcctcgg acgcctcgac
     2041 ccgctcgagg aggtcggagt tggacagggc gatggcggcg tggtcggcga gggcctggag
     2101 ggtggcgagg cggtccgggg cgatcgatcc cggctggcgg tagctgaccg cgagggtgcc
     2161 gatgacgtcg ccgccggggg cacggagggg tgcggcggcc atcgcgccca ggcccatgcg
     2221 ctcggcgagg tcgaggtcgt cggcctcgcg cgggatgcgc gggtccgtcg cgtagctcgg
     2281 cgtccagacg acgcggccct gcccggcggc aagcccattg atgccgccat tgatcgggaa
     2341 ttgctggctt cgcagccagt cgcgcgccct gtcgtccatc ccgcccgcga ccaccatcgg
     2401 cgtcaggtac gtgcggtcgt ccgccatccg cgtgaggtgg gcgccgtcgg acgcgagcac
     2461 gcgccgcgtc tcctccacga tgagcccgag gatcgcgtcc gcgtcgcgga tcgacgtgac
     2521 gcgggccgcg atctcgcgca gggtccgttc ggtctcggct cgtcgggccg tctcctccct
     2581 ggacctgccg agctcgcgaa tgagctccgc gttgtggatg gcgatggtcg cgaggtccgc
     2641 gagcgccgcg agcagcgcgc cctcctggtc gccgaactgg ccggtgtcgc cggagaacac
     2701 cgcgagcgtg ccgaggacga cggcctcgcc gaccagcggc gcgaaggcga tcgaccggat
     2761 gccgacgcgc tcgaagaact cgcggttggg cgggccgtcc gcgaagcgct catccgcgcc
     2821 gtagtcgtcg gtgacgagga cctggcgctc ccggatggcg aggtgcacgc ccgatccccc
     2881 gacgcccagc gactgccact cggtgcgcgt ggcgtcgtcg atgcccgcgt catgtgccca
     2941 gcggacctcg ccggtcgcgg ggtcgaggag gtcgatgacc gcgcccgacg acccgagcag
     3001 ccgggccgcc tcgtcgacga cgtcctggag cagctcggcc gggtcctgga tcgtcatcat
     3061 ccgccgcgcg atctcgcgca gggcccgctc ggcgtccgcg gtgcgctcaa gggcacgctg
     3121 cgacgaggcg aggcggtcga ccagccgggc attcgcgagc gcgacggcgg cctgcgcggc
     3181 catcgcatcc agcagccgca gctcgtcgtc gccgaacgcg tccttctcgc ggtgcacggc
     3241 ctggaacacg cccatcaggc cgtcctcgcc gaccaccgga gccgtcacca ccgagagcgc
     3301 gccgatcgcc cggacgccct cgtcgccctc ggggtagtga cggaagcgcc ggtcgttcag
     3361 gtagtcgccg ctggcgaccg gacggccctc gcggacggcg aggccggcga ggccgatggg
     3421 ctcctcgtcg gggggcgcgt cgagcaccgg cgggtcggtg aacggctcgc cctcggggta
     3481 ggtccacagg acggcaccct tcacgggctc gatgaggtcg agccgcgccc gctcggcgcc
     3541 gagcaggcgg gcggcctcgt ggacgattcg cagcaacagg tcgccggggt cgctgccgat
     3601 cgtggccagc cgtccgccga tctcgcgcag ggtccgctct tcctcggcgc ggcgggccag
     3661 cgcctccctc gacctcccca gctcctcgat caggcgcgcc cggccgaggg ccaccgccga
     3721 ctggccggcg atggtctcga gcagtgcggc gtcgtcgggg ccgaaggcgt ccaccttcgt
     3781 cgaccagacc gtgatcgcgc cgaacgggcc ctggtcgccg tggagcggcg tcgcgatgac
     3841 gccgtggatg cccttcgtgc ggacgtaggt gtcgggcccc ttcccgtgga ccaggttcgg
     3901 atcgctgatg tagtcgcggc tgatgtaggt ctcgccgctg acgacggcct tgccggacgc
     3961 tccgacctcg aggctgtcgt cggggtcctt gggccactcg gtctcgagga tctgctcctc
     4021 gcccgacgcg agcaggccgc gcagcagccg cgcctcgggg tccacgatgt cgatgcgggc
     4081 gccgtcgccg ttgagcaggc ggagggcctc gtcgatcgtg aactggacga ccgactcggg
     4141 gtcgcgggcg ccggagatcc gcgtcccgag ctctcgcagc gagcgttcga cgacggcctg
     4201 gcgctccccg gcctggcgcg agcggtcgag ctcggtgatc agccgggcgt tcgccatcgc
     4261 gaacgcggcg tgctccgcga gcgaacggac gagcgcgatc tggggcgcgc tgaacgcgtc
     4321 cgggcgccgc gagaacgtgc ccagcgcgcc gaacacctcc atgcccgcga cgaggggcgc
     4381 gacgacgagc gaccgcaggc cgacctcgtc gacgaagcga tccggccccg tcgcatggct
     4441 gaacgaccgg tcctcgacgt agtccctggt gacgaccatc cggcgctcgg cgacggcctt
     4501 gccgaacatc ccggtgccga tcggcagccg cagctcccgg atccagtggc cgggctccgg
     4561 atcgacgatg cccgcggagt gggcgaagtg gaggatcccg gtgtccgggt tgagcaggta
     4621 gaggaacgc
//
LOCUS       DBNHXO010000251         2732 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1259419, whole genome shotgun sequence.
ACCESSION   DBNHXO010000251 DBNHXO010000000
VERSION     DBNHXO010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1259419"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="AC162A_05190"
     CDS             <1..419
                     /locus_tag="AC162A_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR5010268.1"
                     /translation="RRLPSERRVRAGDDVLVLVVHASPGSQTRGFDQALDANVVFERA
                     AATDARVICVGHTHVPEVRDLGWKVIVNDGSAGYVFDGDPTASWALVTIKDGAVDAEI
                     RRTEFDVLTVANAISERGLPGDVYRAATVRTGKLVR"
     gene            416..1687
                     /gene="fabF"
                     /locus_tag="AC162A_05195"
     CDS             416..1687
                     /gene="fabF"
                     /locus_tag="AC162A_05195"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR5010269.1"
                     /translation="MTWAWPSAPRRVVVTGMGTVTPLGNDVASTWAGLIAGRSGVARI
                     ANFDASRLGVQIAAELKDFDPSDILDRKEMRRTDRYIQFALVCAHQALVHAGLPPRLD
                     DDMGARSGVIIGSGLGGVSTLFDNVLVMAERGPDRISPFFIPMGIANVGSGQVAIATG
                     ATGPNFATVSACATGGHALGEAWETIRRGDADLMIAGGVEAGIHEATMGGFHSMKALS
                     SRNEDPEAASRPFDQDRDGFVMGEGGGALVLEALEHAEARGATPLVELVGYGATADAS
                     HITLPAPGGIGAVRAAQRALEKAGMEPAEIDHVNAHATSTPEGDKAELQAIRTIFGSH
                     APRVAVSANKSMLGHTLGAAGAIEAIATIQAIREGCIPPTINLAAPDEGYEDLDLTPN
                     VATSRPVRAAISNSFGFGGQNTAVIFRRWEG"
     gene            1723..2379
                     /gene="accB"
                     /locus_tag="AC162A_05200"
     CDS             1723..2379
                     /gene="accB"
                     /locus_tag="AC162A_05200"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR5010270.1"
                     /translation="MSESGLADGRAAVDPVAVGPGDGGSDEGDAVAVEAIETDDAQGG
                     NGAAGEATPAPVLAAADASLLSLVDRLTAVLERSDLGELEVAAGGTTIVLRAPSAVER
                     PVPVAMAAPAEAPAAGRDAQAGPTAVPTSPAIAPAEAKPSVKAPLTGIFYAAPSPGAT
                     PYVAIGDHVAVGQIIGLIEAMKLFNEIKSDMAGRVTRVCVETGALVKAKQPLIEVEAR
                     "
     gene            2376..>2732
                     /locus_tag="AC162A_05205"
     CDS             2376..>2732
                     /locus_tag="AC162A_05205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HIR5010271.1"
                     /translation="MSESHAVRLTGWGRYAPSQVLTNADLERMVDTSDEWIVSRTGIR
                     ERRVAAAHETTASMGAVAGLRAIRTAGIEPDDIDVIILATLTPDYWMPSTAALVKEAI
                     GNTKAAAFDVMAACSGF"
BASE COUNT          406 a          947 c         1017 g          362 t
ORIGIN      
        1 tgcgccggct gccgtccgaa cgccgggttc gcgccggcga cgacgtgctg gtcctcgtgg
       61 tccacgcctc gcccggctcg cagacacggg gcttcgacca ggccctcgac gcgaacgtgg
      121 tgttcgagcg cgcggccgcc accgacgctc gcgtgatctg cgtcggccac acccacgtcc
      181 cggaggttcg cgacctgggc tggaaggtca tcgtcaacga cgggtccgcg ggctatgtgt
      241 tcgatggcga ccccaccgcg tcgtgggcgc tggtgacgat caaggacggc gcggtcgacg
      301 cggagatccg caggaccgag tttgatgtgc tcacggtcgc caacgcgatc tcggagcgcg
      361 gcctgcccgg cgacgtctat cgcgcggcca ccgtccggac cgggaagctc gttcgatgac
      421 ctgggcctgg ccgtcggcgc cgcgacgggt ggtcgtgacc ggcatgggca cggtcacgcc
      481 gctcggcaac gacgtggcat cgacctgggc cggtctcatc gccgggcgat cgggcgttgc
      541 ccggatcgcg aacttcgatg cgtccaggct gggcgtccag atcgccgccg agctcaagga
      601 cttcgacccg tcagacatcc tcgaccgcaa ggagatgcgg cgcacggacc ggtacatcca
      661 gttcgcgctc gtgtgcgccc accaggcgct cgtccatgcg gggttgccgc ctcgcctgga
      721 cgacgacatg ggcgcgaggt caggcgtgat catcggctcg ggcctgggcg gggtgtccac
      781 gctgttcgac aacgtcctgg tcatggccga gcgaggaccc gaccggatct cgcctttctt
      841 catcccgatg ggcatcgcga acgtgggctc cgggcaggtc gccatcgcca cgggcgcgac
      901 cggccccaac ttcgcgaccg tgtcagcgtg cgcgaccggc ggccacgccc tgggcgaggc
      961 ctgggagacg atccggcgcg gcgatgccga cctgatgatc gccgggggcg tcgaggcagg
     1021 gatccacgag gccacgatgg gcggcttcca ctcgatgaag gcgctgtcga gccgcaacga
     1081 ggaccccgag gcggcgtcgc gcccgttcga ccaggaccgc gacgggttcg tgatgggcga
     1141 gggaggggga gcgctggtgc tcgaagcgct cgaacatgcc gaggcgcgcg gcgcgacgcc
     1201 actcgtggag ctcgtgggct atggggccac cgcggacgcg tcccacatca cgttgccggc
     1261 gccgggcggg atcggcgcgg tgcgcgcggc acaacgtgct ctcgagaagg cgggcatgga
     1321 gccggcggag atcgaccacg tgaacgccca cgcgacgtcg accccggaag gcgacaaggc
     1381 ggagctgcag gccatcagga cgatcttcgg cagtcacgcg ccgcgggtcg cggtgagcgc
     1441 caacaagtcg atgctcggcc atacgctcgg cgcggcgggc gcgatcgagg ccatcgccac
     1501 gatccaggcc atccgcgagg gctgcattcc gcccaccatc aacctcgcgg cgcccgacga
     1561 gggatatgag gacctcgacc tcacacccaa cgtcgcgacg agccggccgg tgcgggcggc
     1621 gatctcgaac tcgttcgggt tcgggggcca gaacaccgcg gtcatcttcc ggcggtggga
     1681 aggatgagcg cgccgaccga cgaaggccgg gaggaggagc cgatgagcga gtccggtctc
     1741 gccgacgggc gcgccgccgt tgacccggtg gcggtcgggc cgggcgatgg cgggtccgac
     1801 gagggggacg cggtggcggt tgaggcgatc gagaccgacg atgcacaggg tggcaacggc
     1861 gcagcaggcg aggcgacgcc agcccccgtc ctcgccgccg ccgacgcgtc gctgctttcg
     1921 cttgtcgacc gcctgaccgc ggtcctcgag cgcagcgatc tcggcgagct cgaggtggcc
     1981 gccgggggga cgacgatcgt gctccgggcg ccgtcggccg tcgagcgccc cgtcccggtc
     2041 gcgatggccg ccccggccga ggctccggcg gccggccggg acgcgcaggc cggtccgacg
     2101 gccgtgccca cgagcccggc gatcgcgccc gccgaggcca agccgtcggt caaggcgccc
     2161 ctgaccggga tcttctacgc cgcaccgtcg cccggcgcca cgccgtacgt ggcgatcggc
     2221 gaccacgtgg cggtcggcca gatcatcggc ctgatcgagg ccatgaagct gttcaacgag
     2281 atcaagagcg acatggctgg gcgtgtcacc cgggtgtgtg tggagaccgg cgcgctcgtg
     2341 aaggcgaagc agccgctcat cgaggtggaa gcacgatgag cgagtcgcac gcggtccgcc
     2401 tcaccggctg gggccgctac gccccgtcgc aggtgctgac caacgccgac ctcgagcgga
     2461 tggtcgacac gtccgacgag tggatcgtgt cccggaccgg aattcgcgag cggcgcgtgg
     2521 cggccgcgca cgagaccacg gcgtcgatgg gcgccgtcgc cggcctgcgg gcgatccgga
     2581 ccgcgggcat cgagcccgac gacatcgacg tcatcatcct cgccacgctc acgcccgact
     2641 actggatgcc ctcgacggcg gcgctcgtca aagaggcaat cggcaacacg aaggcggcgg
     2701 cgttcgacgt gatggccgcc tgctcgggct tc
//
LOCUS       DBNHXO010000252         1705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4632614, whole genome shotgun sequence.
ACCESSION   DBNHXO010000252 DBNHXO010000000
VERSION     DBNHXO010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4632614"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="AC162A_05210"
     CDS             complement(<1..1073)
                     /locus_tag="AC162A_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR5010272.1"
                     /translation="MTFLWLGLLALVLVVPILIGIYVWSQRRRRPTAARYSSLSLIRA
                     AMPGASRLRRHLPFALFAAGLAALVISLGRPVVVLSVPANQATIILAMDVSGSMCSTD
                     IDPTRLEAAEQAAIGFVTSQASGSEIGLVAFSGLAAVIEPPTSDQQALVDAIRSLTTG
                     RRTAIGSGILASIDAIAEVDPNVAQADLPGRPGTPPEPVVEGAYVPDIVVLLTDGANN
                     AGTPPAEAAQQAVDRGVRVYTIGFGSAAGGELEPSCRQQFIGREPAGAGGFGGGGFGG
                     GGGQGGFRRGIDEETLRSVADLTGGEYYPAESASELEQVFRDLPTTTITKAEPVEVSA
                     GFVALGGLLAGFALLLGRAWRPLP"
     gene            complement(1070..>1705)
                     /locus_tag="AC162A_05215"
     CDS             complement(1070..>1705)
                     /locus_tag="AC162A_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR5010273.1"
                     /translation="VVVGLVAFSDAGIAVHAPTSDQADILGAIDRLTPARGTSVGQGL
                     LSALDAIEKARADTPADTYSNRSAAPSETFAPVDPASDASTVIVLLSDGENNEDPDPL
                     EMAQEAADRGIRIVTIGVGSAGGADLDLDGFKVHTQLDEATLTQIAELTGGSYHAADD
                     PDGVEAIYTDLQPRLVLRSEPLEVTALFAALGLALLFAGGVMSLAWAGRLP"
BASE COUNT          236 a          630 c          621 g          218 t
ORIGIN      
        1 gggagcggcc gccaggcgcg gccgaggagc agcgcgaacc ccgcgagcag ccccccgagg
       61 gcgacgaacc ccgcgctgac ctcgaccggc tcggccttcg tgatcgtcgt cgtcggcagg
      121 tcgcggaaca cctgctcgag ctcgctggcg ctctccgccg ggtagtactc gccgcccgtg
      181 aggtcggcca cggaccgcag ggtctcctcg tcgatgccgc gccggaaccc accctggccg
      241 ccgccaccgc cgaacccgcc gccaccgaac ccaccggcgc ccgccggctc ccggccgatg
      301 aactgctgcc ggcagctggg ctccagctcg cccccagcgg ccgacccgaa cccgatcgtg
      361 tagacgcgca ccccgcggtc gacggcctgc tgcgccgcct cggccggcgg ggtccccgcg
      421 ttgttcgccc cgtctgtcag cagcacgacg atatccggta cgtacgcgcc ctcgaccacg
      481 ggctccgggg gcgtgccggg gcggcccggg aggtcggcct gggcgacgtt ggggtccacc
      541 tcggcgatcg cgtcgatcga ggccaggatc ccgctgccga tcgcggtccg gcggccggtg
      601 gtgaggctac ggatcgcgtc cacgagcgcc tgctggtcgc tggtcggcgg ctcgatcacc
      661 gcggcgagcc cgctgaacgc aacgaggccg atctccgacc ccgacgcctg gctggtcacg
      721 aagccgatcg ccgcctgttc ggccgcctcg aggcgggtcg gatcgatgtc cgtcgagcac
      781 atgctccccg acacatccat cgcgaggatg atcgtggcct ggttcgctgg aacgctgagc
      841 acgacgaccg gccggccgag tgagatcacc agggccgcga ggccggccgc gaacagcgcg
      901 aacgggaggt ggcggcgcag gcgcgatgcg ccgggcatcg ccgcccggat caacgacagg
      961 ctggaatagc gggccgcggt cggtcgccgc cggcgctggc tccacacgta gatcccgatc
     1021 aggatcggga cgacgaggac gagcgcgagc agccccagcc acaggaacgt cacggcagcc
     1081 tccccgccca cgcgagggac atcacgccgc ccgcgaacag cagcgccagc ccgagcgcgg
     1141 cgaacagcgc ggtaacctcg agcggctcgc tgcgcaggac gagacgtggc tggaggtccg
     1201 tgtagatggc ctcgacgccg tcgggatcgt cggcggcgtg gtacgagccg ccggtcaact
     1261 cggcgatctg ggtgagggtc gcctcatcga gctgggtgtg gaccttgaaa ccgtcgaggt
     1321 cgaggtcagc gccgcccgcg ctgcccacgc cgatggtcac gatccggatc ccgcgatccg
     1381 cggcctcctg ggccatctcg aggggatccg ggtcctcgtt gttctcgccg tcgctcagca
     1441 ggacgatgac cgtcgacgcg tcgctggcgg ggtcgaccgg ggcgaacgtc tcggacggcg
     1501 ccgccgaccg gttgctgtag gtgtcggcgg gcgtgtcggc cctcgccttc tcgatcgcgt
     1561 cgagcgcgga cagcagcccc tgcccgaccg acgtgccgcg ggcgggtgtg aggcggtcga
     1621 tggcgcccag gatgtcggcc tggtcgctgg tcggcgcgtg gaccgcgatc cccgcgtcgc
     1681 tgaacgccac gaggccgacg acgac
//
LOCUS       DBNHXO010000253         2638 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_466245, whole genome shotgun sequence.
ACCESSION   DBNHXO010000253 DBNHXO010000000
VERSION     DBNHXO010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_466245"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="AC162A_05220"
     CDS             complement(<1..812)
                     /locus_tag="AC162A_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5010274.1"
                     /translation="MTAIDTGTEGRPERRAVAVAPDFDATPSVRHARSVEISVVTDVP
                     AGAGAVAFPVGSDGTGASRLGLELPALTAAGFDGNPGQTHALVGGDGPLRVAVGVGDP
                     SSIDAHGIRDAAAAFGLATRRQARLAVRIPILAHVDAGTAAQMIVEGILLARYTYEPL
                     KGTPAAKRVESIALVADDADRAVVTRGAERGRHMAGAAMLARDLANTPPAHLTAPRIA
                     DVAVAIGADRGLEVEVMDQAELTRLGCGGLLGVNGGSADEARMIKLTYRPTAG"
     gene            complement(992..>2638)
                     /locus_tag="AC162A_05225"
     CDS             complement(992..>2638)
                     /locus_tag="AC162A_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HIR5010275.1"
                     /translation="ARLATTRPLDVIEGPLMAGMNVVGDRFQSGRMFLPQVVKSARVM
                     KKAVAHLIPYLEEQREGTGRRAGTIVMATVKGDVHDIGKNIVGVVLGCNDYEVIDLGV
                     MVPAARILESARDHNADLIGLSGLITPSLDEMVHVASLMEADGMTTPLLIGGATTSRI
                     HTAVKIEPSYSSPVVHVADASRAVAVAGSLIDRDGRDAYAAGIRAEYEQVRRDRESRR
                     DRETRLTLEAARANRLRLDWTQAPPRPSFLGVRALEDYPLAELVERIDWTPFFATWEL
                     RGAWPTILSDPTVGAAARELHADATALLARIVEDKSLTASGVVGFWPANSTEDDDIVV
                     FVDETRTTEIARLHAVRQQMAKPDGRPNVSIADFVGPTGVADYIGGFAVTAGKGTTDL
                     VAAFEAAHDDYSAIMARALSDRLAEAFAERLHELVRRELWGYARDETLSNDDLIAERY
                     QGIRPAPGYPATPDHSAKPVLFDLLDATERVGIELTESWAMLPAASVSGLYLWHPGSH
                     YFGIGRMGRDQLEDYARRAGVSDAEALRRLGPNLADDATA"
BASE COUNT          348 a          929 c          995 g          366 t
ORIGIN      
        1 cccgccgtcg ggcggtaggt gagcttgatc atgcgcgcct cgtcggcgct gcccccgttg
       61 acgccgagca ggccaccgca gccgagccgg gtcagctccg cctggtccat gacctccacc
      121 tcgaggccac ggtcggcgcc gatcgcgacg gcgacgtcgg cgatgcgcgg ggccgtgagg
      181 tgagccggcg gggtgttcgc gaggtcccgg gcgagcatcg cggcgccggc catgtgccgg
      241 ccgcgttcgg cgccgcgggt cacgacggcc cggtcggcat cgtcggcaac cagggcgatg
      301 gactcgacgc gtttggccgc cggggtcccc ttgagcggct cgtaggtgta gcgggcgagc
      361 aggatgccct cgacgatcat ctgggccgcg gtgccagcgt ccacgtgggc gaggatcggg
      421 atgcggacgg cgaggcgggc ctggcgtcgg gtggcgagcc cgaaggcggc cgcggcgtca
      481 cggatgccgt gagcatcgat gctggatggg tcgcccacgc caacggccac gcgcagcggc
      541 ccgtccccgc cgacgagcgc atgcgtctgg cccgggttgc cgtcgaaccc ggcggccgtc
      601 aaggcgggca gctcgaggcc gagccggctc gcaccggtgc cgtcggagcc gaccgggaac
      661 gcgacggccc cggcgccggc ggggacgtcg gtgaccacgg agatctcgac ggaacgcgcg
      721 tgccggaccg acggcgtcgc atcgaagtcg ggagcaacgg cgacggcgcg gcgctccggc
      781 ctgccctcgg ttccggtgtc gatagcggtc atcgccgacc tccaggggtc agggtggggc
      841 agcgggcgag agtcggcggc gcaggccccg cacggcggcc gtcgcgtccc gatgccggtg
      901 ccgcaacccc gcggcgccgc gcaccgcagt atcgcccacg tcgccgtgcg ccaatagggt
      961 gattcgccgg cgccttggtc gatcagcccg ttcaggcggt ggcgtcgtcc gccaggttgg
     1021 gtccgaggcg gcgcaacgcc tcggcgtcgc tcacgccggc gcgccgtgca tagtcctcga
     1081 gctggtcgcg gcccatgcgc ccgatcccga agtagtggct gcccgggtgc cacaggtaca
     1141 acccggagac cgaggccgcc ggcagcatcg cccacgactc ggtgagctcg atcccgacgc
     1201 gctcggtcgc atcgaggagg tcgaacaaca cgggcttggc cgagtggtcc ggcgtggccg
     1261 gatagccggg cgccggccgg atgccctggt agcgctcggc gatgaggtcg tcgttcgaca
     1321 gcgtctcgtc gcgtgcgtag ccccacagct cgcggcgaac gagctcgtgg aggcgctcgg
     1381 cgaacgcctc cgcgagacgg tccgacaggg cgcgggccat gatcgcggag tagtcgtcgt
     1441 gcgccgcctc gaacgccgcg accaggtcgg tcgtgccctt gccggcggtc acggcgaacc
     1501 cgccgatgta gtccgcgacg ccggtcggac ccacgaagtc ggcgatcgag acgttcgggc
     1561 ggccgtcggg cttggccatc tgctggcgca cggcgtggag gcgggcgatc tcggtcgtcc
     1621 gggtctcgtc aacgaacacg acgatgtcgt cgtcctcggt cgagttggca ggccagaatc
     1681 cgaccacgcc ggacgcggtg agcgacttgt cctcgacgat gcgggcgagg agcgccgtcg
     1741 catctgcgtg cagctcgcgg gccgccgccc cgacggtggg atccgagaga atcgtcggcc
     1801 acgcgccgcg cagctcccag gtggcgaaga acggcgtcca gtcgatgcgc tcgacgagct
     1861 cggccagtgg gtagtcctcg agcgcccgga cgcccaggaa cgatgggcgc ggcggggcct
     1921 gtgtccagtc gagccgcagg cggttggcgc gcgccgcctc gagggtgagg cgcgtctccc
     1981 ggtcgcgccg gctctcgcgg tcgcggcgga cctgctcgta ctccgcgcgg atccctgcgg
     2041 cgtaggcgtc gcgcccgtcc cggtcgatga gcgagcccgc gacggcgaca gcgcgcgacg
     2101 cgtcggccac gtgcacgacc ggggacgaat aggacggctc gatcttgacc gctgtgtgga
     2161 tgcgcgaggt cgttgcgccg ccgatcagca gcggcgtcgt catcccgtcc gcctccatca
     2221 gcgacgcgac gtggaccatc tcgtccagcg acggggtgat caggcccgac aggccgatga
     2281 ggtccgcgtt gtggtcccgg gcggactcga ggatccgggc cgccggcacc atcacgccga
     2341 ggtcgatgac ctcgtagtcg ttgcagccga gcacgacgcc gacgatgttc ttgccgatgt
     2401 cgtggacgtc gcccttcacg gtcgccatca cgatcgtgcc ggcccgccgt cccgtgccct
     2461 cgcgctgctc ctcgaggtac gggatgaggt gcgcgacggc cttcttcatc acccgtgcgc
     2521 tcttgacgac ctggggcagg aacatgcggc ccgactggaa ccggtcgccg accacgttca
     2581 tgcccgccat cagcggacct tcgatgacgt ccagcggccg cgtcgtcgcc agccgcgc
//
LOCUS       DBNHXO010000254         2106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1259857, whole genome shotgun sequence.
ACCESSION   DBNHXO010000254 DBNHXO010000000
VERSION     DBNHXO010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1259857"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="AC162A_05230"
     CDS             <1..1167
                     /locus_tag="AC162A_05230"
                     /inference="COORDINATES: protein motif:HMM:NF015185.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5010276.1"
                     /translation="EFDHFVPTESVGTTLFLVGVGGAIVGIIIQPTVGYLSDFTISKW
                     GRRKPYIVFGSLLDVVFLAGIATSNTVLMLAAFVLLLSVSTNIARGPFQGYVPDLVAE
                     PQVGLASGLVGLMQIVGNVTGYLLVSLSVASGRMELSLLAVAIVELVTMASVVLRVGK
                     GMPPKPREGRSWPRIAREAWATDILQERSYVWLLVSRFLFLTGGALLANFVLIYMART
                     FGMSKAEANSTNVTLLIVVVVANVIAIIPSSRLSDRLGRKPLIWAACAIGGVGIAIAA
                     LAPSVTVAYVGAALFGAANGTFLAVDWALMTDIIPRASAGRYMGLSNVATGAATPIAI
                     AIGGVVLDGVTRAGGFETGPRIVFALGVVLYAFAAVTLRPVVEPERHAALAPAA"
     gene            complement(1171..>2106)
                     /locus_tag="AC162A_05235"
     CDS             complement(1171..>2106)
                     /locus_tag="AC162A_05235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HIR5010277.1"
                     /translation="LRREATRIREPHRLAAMLILAVVFALTMAGMWARGENAGGDAQA
                     YWAAVRIWLEGGNPYHPTGPFLPYVYAPWMLPLFVPWALLPWEVAWFVWRGGTILLLL
                     WSIRWAYTRRPLPTAILILILAFPIAANLDTGNINLLLALALFGAQFLGPKAGGLIWG
                     LATWMKWVPALLWPVLQPKARGWGLIFLALSALLSLAMLPMTIIQLQTLFGFGTRPVR
                     ADFLVFIWSAVPWWWRHRDPFAFLRPSAWREASAFQLARARRWWTRFRADPREATGLA
                     WTRLRVAIRRTVGLEDRRRRDRMAEAAVTVPEPLD"
BASE COUNT          312 a          740 c          747 g          307 t
ORIGIN      
        1 gagttcgacc acttcgtgcc caccgagagc gtcgggacca cgctgttcct ggtcggggtc
       61 ggcggggcga tcgtggggat catcatccag cccaccgtcg gctatctcag cgacttcacc
      121 atcagcaagt ggggcaggcg caagccgtac atcgtgttcg gctccctgct cgacgtcgtg
      181 ttcctggccg gcatcgcgac gtccaacacg gtgctgatgc tcgccgcgtt tgtcctgctc
      241 ctcagcgtct cgaccaacat cgcgcgcggg cccttccagg gctacgtccc ggacctcgtc
      301 gccgagccgc aggtcgggct cgccagcggg ctcgtcgggc tgatgcagat cgtgggcaac
      361 gtgacgggct acctgctcgt ttcgctctcg gtggcctccg ggcgcatgga gctctcgttg
      421 ctcgcggtgg cgatcgtcga gctggtcacg atggcgagcg tcgtcctccg cgtcgggaag
      481 ggcatgccgc cgaagccgcg cgagggccgc tcctggccgc ggatcgcgcg cgaggcgtgg
      541 gcgacggaca tcctccagga acggtcctac gtctggctgc tggtgtcccg tttcctgttc
      601 ctgaccggcg gcgccctgct cgcgaacttc gtcctgatct acatggcgcg gacgttcggg
      661 atgtcgaagg ccgaagccaa cagcacgaac gtcacgctgc tgatcgtggt cgtcgtcgcg
      721 aacgtgatcg cgatcatccc gtcgtcccgg ctctcggacc ggctggggcg caagcccctg
      781 atctgggccg cctgcgcgat cggcggggtg ggcatagcga tcgcggcgct cgcgccgtcc
      841 gtgacggtcg cgtacgtcgg cgccgcgctc ttcggggccg ccaacggcac gttcctcgcg
      901 gtcgactggg cgctgatgac cgacatcatc ccgcgcgctt cggccggccg gtacatgggg
      961 ctctcgaacg tcgcgacggg ggccgcgacg cccatcgcaa tcgccatcgg cggtgtcgtc
     1021 ctcgacggcg tgacgcgggc aggaggcttc gagaccgggc cacggatcgt gttcgcgctc
     1081 ggtgtcgtgc tctacgcctt cgcggccgtg accctgcgac cggttgtcga gccggagcgc
     1141 cacgcagccc tcgcgccggc ggcctgaggt tcagtcgagg ggctcgggca ccgtcactgc
     1201 ggcctcggcc atccggtccc ggcgccgccg atcctcgagc cccaccgtcc gccggatcgc
     1261 gacccggagg cgcgtccacg ccagcccagt ggcctcgcgc ggatcggcgc ggaagcgcgt
     1321 ccaccagcgg cgtgcgcgcg ccagctggaa ggccgaggcc tcgcgccacg cggatggccg
     1381 caggaacgcg aacggatcgc gatggcgcca ccaccacggg acggccgacc agatgaagac
     1441 caggaagtcg gcgcgcacgg gtcgcgtccc gaaaccgaac agcgtctgga gctggatgat
     1501 cgtcatcggc agcatcgcga gcgacagcag ggcggacagc gcgaggaaga tgaggcccca
     1561 gccgcgggcc ttcggttgga gcaccggcca cagcagcgcg ggcacccact tcatccacgt
     1621 cgcgaggccc cagatcagcc cgccggcctt cggacccagg aactgggcgc cgaacagggc
     1681 gagcgcgagc agcaggttga tgttgcccgt gtcgaggttg gcggcgatcg ggaaggcgag
     1741 gatcaggatc aggatcgccg tcggcagggg gcgtcgcgta tatgcccagc ggatcgacca
     1801 cagcagcagc aggatcgtcc cgccgcgcca gacgaaccag gcgacctccc acggcagcag
     1861 cgcccagggc acgaacaggg gcagcatcca cggcgcatag acgtacggca ggaacgggcc
     1921 ggtgggatgg tatggattgc cgccctcgag ccagatgcgg accgccgccc agtacgcctg
     1981 cgcgtcgccc ccggcgttct cgccacgggc ccacatgccc gccatcgtga gggcgaagac
     2041 cacggcaagg atgagcatcg ccgccaggcg atggggctcg cggatccgcg tcgcctcgcg
     2101 ccgcag
//
LOCUS       DBNHXO010000255         7312 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2052746, whole genome shotgun sequence.
ACCESSION   DBNHXO010000255 DBNHXO010000000
VERSION     DBNHXO010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 7312)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7312)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7312
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2052746"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1927)
                     /locus_tag="AC162A_05240"
     CDS             complement(161..1927)
                     /locus_tag="AC162A_05240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR5010278.1"
                     /translation="MTAEDPAHTPTLAERVRPLIPRYVPITVWARSYPRAWLRPDLLA
                     ALTSWVVMVPVALAYAGLAGVPPEIGLTTAFAAMTVYAVFGTSRHLKVTVSSTMAIMS
                     AAVVVDMAGGDPSLFLVLTSALALTVGLLLTAGGITKLGFIADFLTKSVVTGFIFGLA
                     ITILVGQLPKILGVASVSGSVPDQLRQLVDELPDTNPWTLAVGLSALVLILVLRAISR
                     KIPGALIALLLGLAAVPLLDLEEHGVTVVGEIATGLPSLGIPVPPVSAIPLLILGAVG
                     IVFLAVGESVGAARGFATRHRYEIDADQELVALGAANIATGLFGGFTADASLSQTATS
                     ESAGAKSQLSSLVTAGLVLATAVFLAPVFANLPNAVLGAIVIAAVIGLMDVSEMRRYW
                     TWRRTDFLIAMVALVGVVLTTVLTGLAIAVLLSVAFLLYRASRPYVAALGRIPGYRAT
                     FGDLTRHPDATQIPDLLVIRLDAPLYFFNANVARTQILALVDAAEPRPHGILIDLAAT
                     ADLDVTSADMLYELVGDLEERSIEVLLAQVKGSVRDRMRKTGLMERIGEDRLYLSLGS
                     GVTDFERRQAAKAPTGDAPAPS"
     gene            complement(1947..2813)
                     /locus_tag="AC162A_05245"
     CDS             complement(1947..2813)
                     /locus_tag="AC162A_05245"
                     /inference="COORDINATES: protein motif:HMM:NF013117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR5010279.1"
                     /translation="MPVPPVDTAGLTSWISAHAIPLLIAAIVLFLVYQWARPALHNVL
                     VRVMHAQSTDLAGDPALLAETDRRVVTLEDLFAKLLRFGIFVAAICVVLAVFDLWSVL
                     AGLGLVLAALTLAGQAIILDYLMGILILTEGQYFKGDVVQLGMIEGTVEEVGLRRTVV
                     RDVRGTVHSVSNGTIRVSSNRTRTYATATVDLDGIADRDVEGVIEIFDAVGRDLAADE
                     VFAPKLQDVPGYAATIRLSSSGATLRLTGRVNPEARNEVEAELRRRVAAGMAAKGIEP
                     IRPGTYGATGAS"
     gene            complement(2846..3658)
                     /locus_tag="AC162A_05250"
     CDS             complement(2846..3658)
                     /locus_tag="AC162A_05250"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5010280.1"
                     /translation="MITEYAPSRRVFLADLGRAGFAVAILGVAGCAAATPASPGRSTA
                     AGSAAPGDAGATTPEPTAESSGAPPASGGSAGPTTWHRVNLGFVSAYILARDGEAAVV
                     DTGVAGSDGAIADALEAAGLGWGNVGHVILTHQHQDHAGSAAAVLEAAPDAVGYAGAE
                     DIPAIAVPRALTAVADGDAVFGLRIVATPGHTKGHVAVLDEAGGILVAGDALGASGGS
                     PTRPGAGFTEDMDTALASIAKLGGLTFETLLVGHGDPIESGAAAKVKALAGG"
     gene            complement(3730..4041)
                     /locus_tag="AC162A_05255"
     CDS             complement(3730..4041)
                     /locus_tag="AC162A_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR5010281.1"
                     /translation="MANFLLTFHGGSMPESKEEQDQVMAAWTGWFGSLGDALVDGGNP
                     ISQARAISPDGSVMDATSAPSGYSIIKATDIDAAVALSKGCPVLAGGAVVVVSETFPV
                     M"
     gene            complement(4175..5866)
                     /locus_tag="AC162A_05260"
     CDS             complement(4175..5866)
                     /locus_tag="AC162A_05260"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     D-xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HIR5010282.1"
                     /translation="MAGGGTYTVGIDFGTASARAVVVDVADGRELGVAVHAYANGVID
                     RRLPAPDDDVVLEPDWALQDAADYVEAVRRAVPAAVADAGVEPRQIIGIGIDFTSCTM
                     LPTTADGTPLSALDTHRRNPHAWVKLWKHHAAQPEADEINRVAAELGEPWLDRYGGRI
                     SSEWFFPKALQILREAPEIYASADRLIEAADWIVWQLTGVETRNSCTAGYKALWSADE
                     GFPDASFFAALDPRFERVVDDKMSRTIAPLGGRAGGLSPEAAGWTGLPTGIAVAVANV
                     DAHVSAPAATVTEPGTMVAIMGTSTCHVILSETAPAIAGMCGVVRDGVVPGLFGFEAG
                     QASVGDLFGWFVEHAVPPAYVDRGANGGPDAHGVLETEAAALAPGESGILALDWWNGN
                     RSTLVDADLSGLLVGMTMATGPAEIYRALIEATAFGTRVIIDALEGGGVAVERVVACG
                     GLPERNRLLVQIYADVTGRTWRVAASAQAPALGAAMFGAVAAGAAAGGYADITAAAAQ
                     MAHLRDEAFEPIPANAAVYDRLYAEYVRLHDLFGRGADPAMKTLKAIRLEALEVR"
     gene            complement(5866..6579)
                     /gene="araD"
                     /locus_tag="AC162A_05265"
     CDS             complement(5866..6579)
                     /gene="araD"
                     /locus_tag="AC162A_05265"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013855631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HIR5010283.1"
                     /translation="MAASALGDLREAVWRANLELVRSGLVTLSFGNASGIDRASGIMV
                     IKPSGVPYVDLQPADMVAVALDDGRVVDGGLRPSSDSPTHLALYRRYADIGGVVHTHS
                     PAATAWAQACRAIPPLGTTHADHFHGAIPVTRQLSDAEVAGSYEAETGAVIVETLDDA
                     GLDAARMPAVLVASHGPFTWGRDATHAAENAVALELVAGIAERTIIINPDIGPIGERL
                     LDRHYGRKHGPDATYGQGR"
     gene            complement(6591..>7312)
                     /locus_tag="AC162A_05270"
     CDS             complement(6591..>7312)
                     /locus_tag="AC162A_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014692399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-arabinose isomerase family protein"
                     /protein_id="HIR5010284.1"
                     /translation="LRTVLTAGGFGAFTDTFEDLDGLRQLPGIAAQRLMADGYGFGAE
                     GDWKSAVLVRLLKVMATGLPGGTSFLEDYTYDLDPASPSVLGAHMLEVCPSIAAGRPR
                     CEIHPLGIGGKADPVRLVFDAAPGGAIVAGLTDLGDRFRLVANVIDIVAAGHELPRLP
                     VARAVWKPRPDMRTAVEAWLSAGGAHHTALSRAIGPEPLIDFAEMAGIELLLIDATTT
                     VAGFRREVRWNQAYHHLARGF"
BASE COUNT         1076 a         2625 c         2645 g          966 t
ORIGIN      
        1 gccgagcacc gagatcagga tcggcggatt cttcacgact ggctctacct cgctcccaca
       61 ccgcggcgcc ccggcgttcg atcgccgaac ggcagacccg cgagtggccg cagcgtacgc
      121 ccccgccgga gcggcccgca aggcggacgt gcgtgccggg tcagctcgga gcaggcgcgt
      181 ccccggtcgg cgccttggca gcctgtcgcc gctcgaagtc cgtgaccccc gatcccagcg
      241 agaggtacag gcggtcctcg ccgatccgct ccatcaagcc ggtcttccgc atgcggtcgc
      301 gcacggagcc tttgacctgg gccaggagca cctcgatcga gcgctcctcg aggtcgccga
      361 cgagctcata cagcatgtcg gccgatgtga cgtcgaggtc cgcggtggcc gcgaggtcga
      421 tcaggatccc gtgcggccga ggctcggccg cgtcgacgag cgccaggatc tgggtccgtg
      481 ccacgttggc gttgaagaag tacagcgggg cgtccaggcg aatcacgagc aggtcgggga
      541 tctgcgtcgc gtcggggtgg cgggtgaggt ccccgaacgt cgctcgatag ccggggatcc
      601 gcccgagggc ggcaacgtac ggccggctcg cccgatacag caggaaggcc accgagagca
      661 ggacggcgat cgcgagcccg gtgaggaccg tcgtgaggac gacgcccacg agcgcgacca
      721 tcgcgatcag gaagtccgtc cgccgccacg tccagtagcg gcgcatctcg ctcacgtcca
      781 tgagcccgat gacggccgcg atcacgatcg cacccaggac ggcgttgggc aggttggcga
      841 acaccggcgc gaggaagacc gcggtcgcga ggacgaggcc ggccgtgacc agcgacgaca
      901 gttgcgactt ggcgcccgcc gactcgctcg tcgcggtctg cgacaggctg gcatcggccg
      961 tgaagccccc gaacaggccc gttgcgatgt tcgcggcgcc cagcgcgacc agctcctggt
     1021 cggcgtcgat ctcgtagcga tggcgggtcg cgaacccgcg cgcggcgccc accgattcac
     1081 cgaccgcgag gaagacgatc cccacggcgc ccaggatcag cagtgggatc gccgacaccg
     1141 gcggcacggg gatgccgagc gagggcaacc cggtcgcgat ctcgcccacc acggtgaccc
     1201 cgtgctcctc gaggtccagc agcggcacgg cggccagccc caggagcaac gcgatcaggg
     1261 ctccggggat cttgcgcgag atcgcgcgca gcacgaggat cagcacgagg gcggagaggc
     1321 cgaccgcgag cgtccacggg ttcgtgtcgg gaagctcgtc gacgagctgg cgcagctggt
     1381 ccgggaccga gccgctgacc gacgccacgc ccaggatctt gggcagctgc ccgacgagga
     1441 tcgtgatcgc cagcccgaag atgaagcccg tcacgaccga cttcgtgagg aagtcggcga
     1501 tgaacccgag cttcgtgatc ccgcccgcgg tgagcagcag cccgacggtc agcgcgagcg
     1561 ccgacgtcag cacgaggaac agcgatgggt cgccgcccgc catgtcgacc acgacggcgg
     1621 ccgacatgat cgccatcgtc gagctcacgg tgaccttcag gtgccggctg gtgccgaaca
     1681 cggcgtagac ggtcatggcc gcgaacgcgg tggtcaggcc gatctccggt ggcacgccgg
     1741 cgaggcccgc gtacgcgagc gcgaccggca ccatcaccac ccacgacgtg agcgcggcga
     1801 gcaggtcggg ccgcagccac gcgcggggat aggaccgggc ccagaccgtg atcgggacgt
     1861 agcgtgggat gagcggccgc acgcgctccg cgagcgtcgg tgtgtgcgca gggtcctcgg
     1921 ccgtcaccgt gacccccgtc gggtcatcag gaggcgcccg tcgctccgta cgtccccgga
     1981 cggatcggct cgatcccctt cgccgccatg ccggccgcca cgcgccgtcg cagctcggcc
     2041 tcgacctcgt tccgcgcctc gggattgacc cggccggtga gccgcagggt cgcgccgctc
     2101 gaggagaggc ggatcgtcgc cgcatacccg ggcacgtcct ggagcttcgg cgcgaagacc
     2161 tcgtccgcgg cgaggtctcg gccgacggcg tcgaagatct cgatcacgcc ctcgacgtcc
     2221 cggtcggcga tgccgtcgag gtcgaccgtc gccgtcgcgt aggtccgcgt ccggttcgac
     2281 gacacgcgga tcgtcccgtt cgacaccgag tggaccgttc ccctcacgtc acggacgacg
     2341 gtgcgtcgca ggccgacctc ctcgaccgtg ccttcgatca ttcccagctg gacgacgtca
     2401 cccttgaagt actggccttc ggtcaggatg aggatgccca tcaggtagtc gaggatgatc
     2461 gcctggccgg cgagggtcag ggcggcgagg accagtccga gcccggcgag gaccgaccac
     2521 aggtcgaaca ccgcgaggac gacgcagata gccgccacga agatcccgaa tcgcagcagc
     2581 ttcgcgaaca ggtcctcgag cgtgacgacc cggcggtccg tctccgcgag cagcgcaggg
     2641 tcgccggcga gatccgtcga ctgggcgtgc atcacgcgca cgaggacgtt gtggagggcg
     2701 ggtcgggccc actggtagac gaggaacagg acgatcgccg cgatcaggag cgggatcgcg
     2761 tgtgcgctga tccaggaggt caggccggcg gtgtcgacgg gcgggacggg catggcgcga
     2821 tcattccatg ccggcgcggg cgctgtcagc cgccggcgag cgccttcacc ttcgccgccg
     2881 ccccggactc gatcggatcg ccgtggccga cgagcagcgt ctcgaaggtc aggccgccga
     2941 gcttcgcgat cgacgcgagc gccgtgtcca tgtcctccgt gaacccggcg ccgggtcggg
     3001 tcgggctgcc gccggacgct ccgagcgcgt ctccggcgac gaggatcccg ccggcctcgt
     3061 cgagcaccgc cacgtggcct ttcgtgtggc ccggcgtcgc gacgattcgc agcccgaaca
     3121 ccgcgtcgcc gtccgcgacg gcggtcagcg cgcggggcac ggcgatggcg ggaatgtcct
     3181 cggcgccggc gtagccgacg gcgtccgggg ccgcctcgag cacggcagcc gcgctgcctg
     3241 cgtgatcctg gtgctggtgg gtgaggatca catgccccac gttcccccag ccgagaccgg
     3301 cggcctcgag cgcgtccgcg atcgcgccat cgctgccggc tacgcccgtg tcgacgacgg
     3361 ctgcctcgcc gtcgcgggcg aggatgtagg cggacacgaa gccgagattc acgcggtgcc
     3421 aggtggtggg tcccgcggac cctccggaag ccggcggcgc cccactcgat tccgccgtcg
     3481 gttccggcgt cgtcgcgccg gcgtcgccgg gggccgccga gccggcggcg gtcgagcgac
     3541 cgggcgatgc gggcgtggcc gccgcgcaac cggcgacgcc gaggatcgcc accgcgaacc
     3601 cggcccggcc gaggtcggcg aggaagacgc ggcggctcgg cgcgtactcg gtgatcatca
     3661 accgatggta cgacggggga tccgccgccg gatcccgccg ccgagccagc gcagggtcga
     3721 gccccgcggc tacatgaccg ggaaggtctc cgagacgacg acgacggcac cgccggcgag
     3781 gacggggcag cccttcgaca gggcgaccgc ggcgtcgata tcggtggcct tgatgatcga
     3841 gtagccgctc ggggcggacg tcgcgtccat gaccgagccg tcgggcgaga tcgccctggc
     3901 ctgcgagatc gggttgccgc cgtcgacgag ggcatccccg agcgagccga accagccggt
     3961 ccaggcggcc atcacctggt cctgttcctc cttggactcg ggcatgctgc cgccgtggaa
     4021 cgtgagcagg aagttcgcca tgtgtccatc tcctcgtctg tgggcatcgt cttcgttcgc
     4081 ccgtgttcac gacgaacgag accaccccga atcgacaggg ccacctgccc ggccccgact
     4141 ggggcttggc aatcgcacgg cggggcgctg accgtcagcg gacctcgagc gcctcgagcc
     4201 ggatcgcctt gagcgtcttc atcgccgggt ccgcgccgcg cccgaacagg tcgtggaggc
     4261 ggacgtactc cgcgtacagg cggtcgtaca cggcggcgtt cgcgggaatc ggctcgaacg
     4321 cctcgtcgcg gaggtgcgcc atctgcgccg cggcggccgt gatgtcggca tatcccccgg
     4381 ccgccgcgcc ggccgcgacc gccccgaaca tcgccgcgcc cagcgcgggc gcctgcgccg
     4441 atgcggcgac gcgccacgtc cgccccgtca cgtcggcata gatctggacg aggaggcggt
     4501 tccgttcggg gagtccaccg caagcgacca cgcgctcgac cgccaccccg ccaccctcga
     4561 gggcgtcgat gatcacccga gtcccgaacg cggtagcctc gatcaaggcc cggtagatct
     4621 cggccggtcc ggtcgccatg gtcatgccca ccaggaggcc cgacaggtcc gcgtcgacga
     4681 gcgtcgagcg gttgccgttc caccagtcga gcgcgaggat cccggactca cccggcgcca
     4741 gggctgcggc ctcggtctcg agcaccccgt gggcgtcggg accgccattc gcgccccgat
     4801 ccacgtaggc cggtggcacc gcgtgctcga cgaaccaccc gaacaggtcg ccgacggacg
     4861 cctgccccgc ttcgaagccg aacaggcccg gcaccacgcc gtcccgcacc accccgcaca
     4921 tgccggcgat cgccggcgcg gtctcggaca ggatgacgtg gcacgtgctc gtgcccatga
     4981 tcgcgaccat cgtgcccggc tccgtgaccg tcgccgcggg cgcggacacg tgggcgtcga
     5041 cgttggcgac ggcgacggcg atgccggtcg gcagcccggt ccagccggcg gcctccggcg
     5101 acagcccgcc ggcccggccg ccgagcgggg cgatcgtccg gctcatcttg tcgtccacga
     5161 cccgctcgaa ccgcgggtcg agcgccgcga agaacgacgc gtccgggaat ccctcgtcgg
     5221 ccgaccacag cgccttgtag ccggcggtgc agctgttgcg cgtctcgacg cccgtgagct
     5281 gccagacgat ccagtcggcg gcctcgatca ggcgatccgc cgacgcgtag atctccggcg
     5341 cctcgcgcag gatctggagg gccttgggga agaaccactc cgacgagatg cggccgccgt
     5401 aacggtcgag ccacggctcg ccgagctccg cggcgacgcg gttgatctcg tctgcctcgg
     5461 gttgggccgc gtggtgcttc cacagcttga cccacgcgtg cgggttccga cgatgtgtgt
     5521 cgagcgcgga gagaggcgtc ccgtcggccg tggtcgggag catcgtgcac gacgtgaagt
     5581 cgatgccgat gccgatgatc tgtcgcggtt cgaccccggc gtcggcgacc gccgcgggca
     5641 cggcccgccg cacggcctcg acatagtcgg ccgcgtcctg gagcgcccag tcgggctcga
     5701 ggaccacgtc gtcgtcgggc gcgggcagcc tgcggtcgat caccccgttg gcgtaggcat
     5761 ggacggcgac cccgagctcc cggccatccg ccacgtccac gacgaccgcg cgtgcggatg
     5821 cggtaccgaa gtcgatgcca acggtgtacg tcccgccccc ggccatcagc ggccctgccc
     5881 gtacgtggcg tcaggcccgt gcttgcgccc gtagtgccgg tcgaggaggc gctcgccgat
     5941 gggcccaatg tccgggttga tgatgatcgt gcgctccgcg atgccggcga cgagctcgag
     6001 ggcgacggcg ttctcggccg catgcgtggc gtcccggccc cacgtgaacg gtccgtgtga
     6061 cgcgaccagc acggccggca tccgggcggc gtcgaggcct gcgtcgtcga gggtctcgac
     6121 gatgaccgcc cccgtctccg cctcgtacga gccggccacc tcggcgtcgc tgagctggcg
     6181 ggtgaccggg atcgcgccat ggaagtggtc agcgtgcgtg gtcccgaggg gcgggatggc
     6241 ccggcacgcc tgcgcccacg cggtcgcggc cggcgaatgg gtatggacca cgccgccgat
     6301 gtccgcgtag cgccggtaca gggcgagatg ggtggggctg tccgaggagg gccgcaggcc
     6361 gccgtcgacc acccgcccat cgtcgagcgc gacggccacc atgtctgccg gctgcaggtc
     6421 gacgtagggc acgccgctgg gcttgatgac catgattccg gaggcccgat cgatcccgct
     6481 ggcgttcccg aacgacagcg tcacgaggcc ggaccggacg agctcgaggt tggcccgcca
     6541 gaccgcctcg cgaaggtcgc ccaaagccga tgcagccatg ccgccgtccc ctagaacccg
     6601 cgtgcgaggt ggtggtaggc ctgattccag cggacctcgc gccggaagcc tgccacggtg
     6661 gtcgtcgcat cgatcagcag gagctcgatg ccggccatct cggcgaagtc gatcagcggc
     6721 tcgggaccga tggcgcggct gagcgccgta tggtgggcgc cgcccgcgct gagccacgcc
     6781 tcgacggcgg tccgcatgtc gggtcgcggc ttccagaccg cgcgggccac gggaagtcgc
     6841 ggcagctcgt gcccagccgc cacgatgtcg atgacgttgg cgacgagccg gaaccggtcg
     6901 ccgaggtcgg tgaggccggc gacgatcgcc ccgcccgggg ccgcgtcgaa cacgagccgg
     6961 accgggtcgg ccttgccacc gatgcccagc gggtggatct cgcagcgcgg gcggcccgcg
     7021 gcgatcgacg ggcagacctc gagcatgtgg gcgccgagca cgctcggcga agcggggtcg
     7081 aggtcgtacg tgtagtcctc caggaacgag gtcccgccgg gcaggccggt cgccatcacc
     7141 ttgagcaggc ggaccaggac ggcgctcttc cagtcgccct ccgctccgaa gccgtacccg
     7201 tcggccatca ggcgctgggc ggcgatgccc ggcagctggc gcagcccgtc gagatcctcg
     7261 aatgtgtcgg tgaaggcccc gaagccaccc gccgtcagca ccgtgcgcag gc
//
LOCUS       DBNHXO010000256         2788 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1656482, whole genome shotgun sequence.
ACCESSION   DBNHXO010000256 DBNHXO010000000
VERSION     DBNHXO010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1656482"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="AC162A_05275"
     CDS             complement(<1..479)
                     /locus_tag="AC162A_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010285.1"
                     /translation="MTADLAVLAPFIAAILVAAAVLVTDFIFPGRRGPALAVAFLGLA
                     IVALLTALVGQDPLPKEAFKNAAGQPSYIVDALTTFLDILFVVIVAMTIAFAPDYLEE
                     RDLPVAEFAAILIFAMSGAMLIAGSADLLVLFLGLELMVLPGYVMAGYHKSDGYSTEG
                     "
     gene            complement(487..2055)
                     /locus_tag="AC162A_05280"
     CDS             complement(487..2055)
                     /locus_tag="AC162A_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011715162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 4 family protein"
                     /protein_id="HIR5010286.1"
                     /translation="MSWLADLHPVTLVTLLPLLGAIVVAFVPSSRPDAVRRVALAFAL
                     AAWVVSLALLAAYVTGVPGFQFVDTYEWIPLFGISYKVGIDGLSLVLVVLTTTLTWIS
                     LLASFGPIQSRVKEYMVSFLVLEVGMIGVFVALDLFLFYIFWELVLVPMYLIIGIWGG
                     SNRIYATMKFVLYTLVGSLLMLVAILATAFTYQAAQAAAGVPDPWHGAFDLVTLTNFA
                     GQGGFSPTFQFLAFGAFFLAFAIKVPMFPFHTWLPDAHVEAPTAGSVILAGVLLKLGG
                     YGFIRFALPLFPDAAHTWAPAIIVLSLIAIIYGAIVALVQPDLKKLVAYSSVSHMGFV
                     TLGIFVFQQQGLQGAILQMVNHGLITGGLFLLVGVIYERTHDRTIAKMGGLSSKVPVY
                     AAAFGFFVFASVGLPGLSGFVGEFLTLVGTFVMIPGAAAIATFVMILAAGYLLWMFQR
                     VAFGELSDFLKGLGGHLTDMRPMEAMTLVPLGALIVILGLFPGLILGIIGPSVQSTLE
                     AVQRAAPIALGLWP"
     gene            complement(2052..>2788)
                     /locus_tag="AC162A_05285"
     CDS             complement(2052..>2788)
                     /locus_tag="AC162A_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010287.1"
                     /translation="YWVWIIGLTVAVMTAFYMWRLMGKTFYGPSHVDPAVEPKIHESP
                     WQMTLPLILLAIPSIFLGIVLTWGGPPLNPGGIGLLEGWLEPVFAQSEAYLHETPAGF
                     ELLGIDGALIIISVAAAALGLVAGIWLDGFFRNGGRPRLVERITVANRATRFLYRASL
                     NKWWFDELNHLLFIVIGGRIANGMWWFDRVIVDGTVNSIGGATRGAGGSLKRIQTGRV
                     QNYALGIAIGLLVMAGSFLVIAGQPR"
BASE COUNT          551 a          919 c          980 g          338 t
ORIGIN      
        1 ccttcggtcg aatagccgtc cgacttgtgg tagccggcca tcacgtatcc gggcaggacc
       61 atgagctcga ggcccaggaa cagcaccagc aggtccgcgg aaccggcgat cagcatggcg
      121 cccgacatcg cgaagatcag gatggcggcg aactcggcca ccggcaggtc ccgctcttcc
      181 aggtagtcgg gcgcgaacgc gatcgtcatg gcgacgatca cgacgaacag gatgtcgagg
      241 aacgtggtca gcgcatcgac gatgtacgag ggctggccgg cggcgttctt gaacgcttcc
      301 ttgggcagcg ggtcctgacc gacgagggcg gtcagcaggg cgacgatcgc gaggcccagg
      361 aacgcgaccg cgagcgccgg tccgcggcgg cccgggaaga tgaagtccgt gacgaggacc
      421 gcggccgcga cgaggatcgc ggcgatgaac ggcgccagga cggcgagatc ggcggtcatc
      481 gtgggctcac ggccacaggc caagggcgat cggggccgca cgctgcacgg cctcgagcgt
      541 ggactggacc gacgggccga tgatgcccag gatcagcccc gggaacaggc cgaggatcac
      601 gatcagcgcg cccagcggca ccagcgtcat cgcctccatc ggccgcatgt cggtgaggtg
      661 gccgccgagg cccttgagga agtccgacag ctcgccgaac gcgacccgct ggaacatcca
      721 cagcaggtag ccggcggcaa ggatcatcac gaatgtcgcg atcgccgcgg cgcccgggat
      781 catcacgaac gtcccgacga gcgtcaggaa ctcgccgacg aacccggaca ggcccggcag
      841 gccgacggac gcgaacacga agaacccgaa tgcggcggcg tagaccggca ccttcgacga
      901 caggccgccc atcttggcga tcgtgcggtc gtgggtccgc tcgtagatca cgcccacgag
      961 caggaacagg ccgccggtga tcaggccgtg gttgaccatc tgcaggatcg cgccctgcag
     1021 gccctgctgc tggaacacga agatcccgag cgtgacgaag cccatgtggc tcaccgacga
     1081 ataggcgacg agcttcttga ggtcgggctg gacgagggca acgatcgcgc cgtagatgat
     1141 cgcgatcagg ctgagcacga tgatcgcggg cgcccacgtg tgcgccgcgt cggggaacag
     1201 cggcagggcg aagcggatga acccgtagcc gccgagcttg agcagcacgc ccgccaggat
     1261 caccgagccg gccgtcggcg cctcgacgtg cgcgtcgggg agccaggtgt ggaacgggaa
     1321 catcggcacc ttgatcgcga aggcgaggaa gaaggcgccg aacgcgagga actggaaggt
     1381 cggcgagaag ccgccctgcc ccgcgaagtt ggtgagcgtc accaggtcga acgcgccgtg
     1441 ccacgggtcc gggacgcccg ccgcggcctg cgcggcctgg taggtgaacg ccgtggccag
     1501 gatcgcgacc agcatcagca gcgacccgac cagggtgtac aggacgaact tcatcgtcgc
     1561 gtagatccgg ttcgacccgc cccagatgcc gatgatcagg tacatcggca cgagcaccag
     1621 ctcccagaag atgtagaaca ggaacaggtc gagggccacg aacaccccga tcatgccgac
     1681 ctcgaggacg aggaaggaga ccatgtactc cttgacccgg ctctggatcg ggccgaacga
     1741 ggcgagcagg ctgatccacg tgagcgtcgt ggtcagcacc acgaggacga gcgagagccc
     1801 gtcgatgccg accttgtacg agatgccgaa cagcgggatc cactcgtagg tgtcgacgaa
     1861 ttggaagccg gggacgccgg tcacgtacgc ggcgagcagg gcgagcgaca cgacccacgc
     1921 cgcgagggcg aacgccagcg ccacccgccg gacggcgtcc ggtcgcgacg acgggacgaa
     1981 cgcgacgacg atcgcgccga ggagcggcag cagggtcacc agcgtgaccg gatggaggtc
     2041 ggcgagccag ctcatcgcgg ctgacccgcg atcacgagga acgagcccgc catcacgagc
     2101 aggccgatcg cgatccccag cgcgtagttc tggacgcggc cggtctggat ccgcttcagg
     2161 ctcccgccgg ccccgcgcgt cgcgccgccg atgctgttca cggtgccgtc cacgatcacg
     2221 cggtcgaacc accacatccc gttggcgata cgtccgccga tgacgatgaa caacaggtgg
     2281 ttgagctcgt cgaaccacca cttgttgagc gaggcccggt acaggaaccg cgtcgcgcgg
     2341 ttggcgacgg tgatccgctc gaccagccgc ggccggccgc cgttgcggaa gaagccgtcc
     2401 agccagatgc cggcgaccag gcccagagcc gcggccgcga cgctgatgat gatcagcgcg
     2461 ccgtcgatgc ccaacagctc gaacccggcc ggcgtctcgt gcaggtaggc ctcggactgc
     2521 gcgaacacgg gctccagcca gccctcgagc aggccgatgc cgcccgggtt cagcggcggg
     2581 ccgccccagg tcaggacgat gcccaggaag atcgacggga tcgcgagcag gatcagcggc
     2641 agcgtcatct gccacggcga ctcgtggatc ttcggctcga ccgcggggtc gacatgcgac
     2701 gggccgtaga acgtcttgcc catcagccgc cacatgtaga acgcggtcat cacggcgacg
     2761 gtgaggccga tgatccagac ccagtaat
//
LOCUS       DBNHXO010000257         1594 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5230172, whole genome shotgun sequence.
ACCESSION   DBNHXO010000257 DBNHXO010000000
VERSION     DBNHXO010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5230172"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="AC162A_05290"
     CDS             complement(<1..452)
                     /locus_tag="AC162A_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5010288.1"
                     /translation="MRTLITGLGEIVSGDIADPRLEADSILIEDGRISAVGRGLDADA
                     DVVIDGKGATAFPGLIDSHVHPVFGDWTPRQRTTDFIESELHGGVTSMISAGEVHLPG
                     RPKDIVGLKALAIVAVRAFANVRPAGVKVHAGAPILELGLEEHDFADLA"
     gene            complement(470..1042)
                     /locus_tag="AC162A_05295"
     CDS             complement(470..1042)
                     /locus_tag="AC162A_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HIR5010289.1"
                     /translation="MAVEVRKWVTFSEEILSEGGRDLEKPLRRVAVAAVLKNPYAGAY
                     SEDISQLIDAGEVLGDELMRRCKAALSDPVEAYGKGGIVGEDGELEHVAAVLHPKFGA
                     PVRGLVDGVSILPSVKKRGGQGAHLDIPVHHIKAMLVRSHFDAMEICVPDAPRADELV
                     VALAVTNGGRPHARVGGLKAEDAIGTDGLH"
     gene            1228..>1594
                     /locus_tag="AC162A_05300"
     CDS             1228..>1594
                     /locus_tag="AC162A_05300"
                     /inference="COORDINATES: protein motif:HMM:NF016430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5010290.1"
                     /translation="MRTPLVERARRGDDVAFGELVDADGDRCFAIAYRILRDVEAAQD
                     AVQQAFLLAWRDLPRLRDIERYDVWLHRLLVNACFEESRRQRRWTSRIRLLPIDGPVS
                     PDPTVSVDDRDLIERAFARL"
BASE COUNT          231 a          571 c          553 g          239 t
ORIGIN      
        1 gccaggtcgg cgaagtcgtg ctcctcgagg cccagctcga ggatgggcgc cccggcgtgc
       61 accttgacgc cggccggccg gacgttggcg aaggcccgga cggcaacgat ggcgagcgcc
      121 ttgaggccga cgatgtcctt gggccggccc ggcaggtgca cctcgccggc cgagatcatc
      181 gacgtgacgc cgccgtggag ctcggactcg atgaagtcgg tcgtgcgctg gcggggcgtc
      241 cagtcgccga acaccgggtg gacgtgggag tcgatgaggc ccggaaaggc ggtcgctccc
      301 ttgccgtcga tgacgacgtc cgcgtccgcg tcgaggcccc tgccgacagc cgagatccgc
      361 ccgtcctcga tcaggatcga gtccgcctcg aggcgcgggt cggcgatgtc gcccgagaca
      421 atctcgccga ggccggtgat cagcgtccgc atacgggtcg tgccggccct cagtgaaggc
      481 cgtcggtgcc gatcgcgtcc tcggccttca acccaccgac acgggcgtgc ggccgcccgc
      541 cattggtgac cgcgagcgcg acgacgagct cgtccgcacg gggcgcgtcg gggacgcaga
      601 tctccatcgc gtcgaagtgc gagcggacga gcatcgcctt gatgtggtgg accgggatgt
      661 cgaggtgggc gccctggccg ccgcgcttct tgaccgacgg caggatcgag acgccgtcga
      721 cgagtccgcg gaccggggcg ccgaacttcg ggtgcagcac cgccgcgacg tgctcgagct
      781 cgccgtcctc gccgacgatc ccgcccttgc cgtacgcctc gaccgggtcg ctcagggccg
      841 ccttgcaccg ccgcatcagc tcgtcgccca ggacctcgcc ggcgtcgatc agctgggaga
      901 tgtcctcgga gtacgcgccc gcatacgggt tcttgagcac cgcggccacc gccacgcgcc
      961 gcagcggctt ctcgagatcc cgaccgccct cgctcaggat ctcttccgag aacgtgaccc
     1021 acttgcggac ttcgacggcc accccgccat cacctctttc gattgcttcc ggtttcatgg
     1081 cagatgatcc cggttggcat gccctgtgcg caaggcgacg ataaccgccg cggccggcac
     1141 gcgcccgagg attgaaccgc tggccccgat cgaccacctt tagtctgcga agcgcccaca
     1201 caagggcgcc gcagacgagg cgcccaggtg cggacaccgc tggtggaacg ggcgaggcgg
     1261 ggcgacgacg tcgccttcgg cgagctcgtg gacgccgacg gcgaccgctg cttcgcgatc
     1321 gcgtaccgga tcctgcgcga tgtcgaggcg gcccaggacg ccgtgcagca ggcgttcctg
     1381 ctggcgtggc gcgacctccc tcgacttcgg gacatcgagc gttacgacgt ctggctccat
     1441 cgcctgctcg tgaacgcgtg cttcgaggag tcccgccgcc agcgacggtg gacgagtcgg
     1501 atcaggttgc tgccgatcga cggaccggtc agccccgacc cgacggtctc ggtcgacgac
     1561 cgagacctca tcgagcgcgc gttcgcccgc ctga
//
LOCUS       DBNHXO010000258         1952 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4237722, whole genome shotgun sequence.
ACCESSION   DBNHXO010000258 DBNHXO010000000
VERSION     DBNHXO010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4237722"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="AC162A_05305"
     CDS             <1..471
                     /locus_tag="AC162A_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR5010291.1"
                     /translation="PEAKGAVIGWGDVHTRAHLYRAILEGLAYALREGAERTVKRTKV
                     PIRELRVSGGGSQSPAAVQLIADVFGLPTSRPHTHETSGLGAAIDAAVGLGLHPSFEV
                     AVASMTRIAETREPRPATHAMYEQLYRDVYLKLYDRLKPLYGEIRRITGYPPVV"
     gene            895..1083
                     /locus_tag="AC162A_05310"
     CDS             895..1083
                     /locus_tag="AC162A_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010292.1"
                     /translation="MLTRATTNAVAYRASSMRRMRRRVVNSTKMYCATNGCTSYLVVD
                     EFGGVAHCEICGYTRRLS"
     gene            1192..>1952
                     /locus_tag="AC162A_05315"
     CDS             1192..>1952
                     /locus_tag="AC162A_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5010293.1"
                     /translation="MTGRTLDRSRLERLMASETAAFERANPRSRELFERAKSSLLDGV
                     PMNWMVKWAGPYPLFVDEASGARFRDVDGHEYVDFCLGDTGAMAGHGPSPTIAAVERQ
                     LRRGITHMLPTEDAIVAGDELRRRFGLRWWQFTLTATDANRFAIRLARHVTGRPKVLV
                     HNHNYHGSVDETFAWIGPDGGVVARRGNLGAPVPPSETTRVVEINDIDALERELAHGD
                     VAVALMEPALTNVGIVLPDPGYHEAVRELTRRHGTL"
BASE COUNT          313 a          715 c          652 g          272 t
ORIGIN      
        1 ccggaggcga agggcgcggt catcggctgg ggcgacgtcc acacgcgggc gcacctgtac
       61 cgcgcgatcc tcgagggcct cgcctacgcc ctgcgggagg gcgcggagcg gacggtcaag
      121 cggaccaagg tgccgatccg cgagctgcgc gtgtcgggcg gcgggtcaca gagcccggcg
      181 gccgtccagc tcatcgccga cgtcttcggg ctgccgacgt cgcgcccgca cacccacgag
      241 acgtccgggc tgggggcggc gatcgacgcc gcggtcggcc tgggcctgca tccgtcgttc
      301 gaggtggctg tcgcgtcgat gacccggatc gccgagacgc gcgagccccg gccggcgacc
      361 cacgcgatgt acgagcagct ctatcgcgac gtctatctca agctctacga ccgcctcaag
      421 ccgctctacg gtgagatccg ccggatcacc ggttacccgc ctgtcgtgtg accgcagtgc
      481 gggcggcaga gtcatgggcg ccgcgctagc actctgactg ctaggcggtg agggatcggc
      541 ggtgcagagg gttgtgagaa gcctccccac ggcgcattca gcactcctgc tgctcaacac
      601 ggcaataggc aggccatcgg cgcgccgtta cgtccgcaat accgtccggc cagcactcgg
      661 ggtgctatgc ggatcgtcct gccccgatcg gacgagttga cagaacggac gatccaaacg
      721 atgattcctg catcgcccga ttgcacgcgg ccacctggtc gtcgccgaac acgcgaaacg
      781 atcgccgcgc cgggcccaac tagatcgccg ctccggcctc cgtatgagtc cggccgttgg
      841 agcggcaagg cgcaagtccc aacccgacgc gcgcccggaa aggaatctct cttcatgctc
      901 acacgcgcca ccacgaatgc cgtggcgtat cgcgccagct cgatgcgccg aatgcggcgc
      961 cgagtggtca actccaccaa gatgtattgc gccaccaacg gctgcaccag ctacctcgtc
     1021 gtcgacgagt tcggcggtgt cgcccattgc gagatctgcg gctacacccg ccgtctgagc
     1081 tagctcgcag ctccggggca cgcgacggcg tcccgccgcg ggccccgacc ccccatcatc
     1141 gacctccaca gcgcgacccc cgaagcgggg ccgccggcat tggaggcgtc gatgacgggg
     1201 aggaccctgg accgatcccg gctcgaacgc ctcatggcga gcgagacagc cgccttcgag
     1261 cgcgccaacc cgcgttcgcg cgagctgttc gagcgagcca agagctcgct gctcgacggc
     1321 gttccgatga actggatggt caagtgggcc gggccatatc cgctgttcgt cgacgaggcg
     1381 agcggcgccc gcttccgtga cgtcgacggc cacgagtacg tcgacttctg cctcggcgac
     1441 accggggcga tggccggcca cggcccctcc cccacgatcg ccgccgtcga gcgccagctg
     1501 cggcggggga tcacgcacat gctgccgacc gaggacgcga tcgtcgcggg cgacgagctg
     1561 cggcgaaggt tcgggcttcg ctggtggcag ttcaccctga cagccacgga cgccaaccgg
     1621 ttcgcgatcc ggctcgcccg ccatgtcacc ggccggccga aggtcctcgt ccacaaccac
     1681 aactaccacg ggtcagtcga cgagacgttc gcctggatcg gccccgacgg gggcgtcgtc
     1741 gcccggcgcg gcaacctcgg ggcgccggtg cccccgtccg agacaaccag ggtcgtcgag
     1801 atcaacgaca tcgacgccct cgagcgcgag ctggcccacg gcgacgtcgc cgtcgcgctc
     1861 atggagcccg cgctgaccaa cgtcggcatc gtgctccccg atccgggcta ccacgaggcg
     1921 gtccgcgagc tcacccgccg gcacgggacg ct
//
LOCUS       DBNHXO010000259         1911 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1657587, whole genome shotgun sequence.
ACCESSION   DBNHXO010000259 DBNHXO010000000
VERSION     DBNHXO010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1657587"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..804)
                     /locus_tag="AC162A_05320"
     CDS             complement(130..804)
                     /locus_tag="AC162A_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly FimT family protein"
                     /protein_id="HIR5010294.1"
                     /translation="MRHAQAGFTLAELAIVAVLVGLFVGGVLSGRAVVQQARIKLTLA
                     EFNGVHAAHLQYLDRYAALPGDDPRATTRWPARAKDGNGDGQLSGTYRDPPPTGDPMA
                     TLTVNGTEGETLNYWWHLRLADLIAVPPANVPIVAQPLNPFAGVIGVQQDAMGFPILA
                     VCQANLPGVIAIAVEGQLDDMSPERGSVRGRRQTAPNQPLADTVAITAYTEADDAQYV
                     LCRRLD"
     gene            complement(1117..1824)
                     /locus_tag="AC162A_05325"
     CDS             complement(1117..1824)
                     /locus_tag="AC162A_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR5010295.1"
                     /translation="MQSKRMQAGFTLVEIAIVLVIIGLLLGGILKGQEMITQARIKNV
                     VNDFNGITAAYFGYQDRYKQVPGDDNQTNVRWADPNPPGGSRNGNGNGVIAGFYQAAP
                     AALTGTAPAANEESNLFWWHLRLAGFVAGATAGPPSWTPPVNALGGFVGVQNGAPGIA
                     AGQPRLAGLVLCSANIPDKIASAVDTQVDDQRSQTGQMYGYLQTAPNLDIEAGPTYAA
                     PNYVESGNNQYVVCKKV"
BASE COUNT          279 a          625 c          688 g          319 t
ORIGIN      
        1 gggcccgaga cgcgcctcga ggcgggcgac gtcctcgtcc tgctcggccg gccggacgcg
       61 ctcgagaagg cggagacgaa gctgctgcag ggctaggcgg ccgagcagga tccgagggcg
      121 gatgccgttt cagtcgaggc ggcggcagag cacgtactgc gcgtcgtcgg cctcggtgta
      181 ggcggtgatc gcgaccgtgt cggcgagcgg ctggttgggc gcggtctgcc ggcggccgcg
      241 cacgctaccg cgctccgggc tcatgtcgtc gagctgccct tcgaccgcga tcgcgatgac
      301 gcccgggaga ttcgcctggc acaccgcgag gatcgggaag cccatcgcgt cctgctgcac
      361 gccgatgact ccggcgaacg ggttgagcgg ttgcgcgacg atcggcacgt tggccggcgg
      421 aaccgcgatc aggtccgcca gccgcaggtg ccaccagtag ttgagcgttt cgccttcggt
      481 gccgttcacc gtcagcgtcg ccatcggatc gcctgtgggc ggcggatcgc ggtatgtacc
      541 gctcagctgg ccgtcgccat tgccgtcctt cgcgcgggcc ggccagcgtg tagtcgcccg
      601 cgggtcgtcc ccgggcagcg cggcatagcg atcgaggtac tggaggtgcg cggcgtggac
      661 gccgttgaac tcggcgagcg tgagtttgat gcgggcctgc tggacgaccg ccctgccgga
      721 caggacgcca ccgacgaaga ggcccacgag caccgcgacg atcgcgagct cggccaacgt
      781 gaacccagcc tgcgcgtgac gcatttccgc aagggtgtgg agggagcgtt gagaatgtgc
      841 gaattctctc gtccgcgctt ccctgcgact gtgacggagt tcgcataacg cgcgccgccg
      901 ccgggaattt gccgacgcgg tgtcacgacg ccgacgtgtg gaagactcgt cgtcgtagcg
      961 gcgtggcgtc gatctcgcga cgcgcgcgaa cggcgacaac gacggccgag gcgcggcgat
     1021 cggcggcgac ttgcgccgcc ttcagccgct gcggccggcg cgggcaacaa aaaagcccgc
     1081 gcatcgcgcg ggctttgaat agcggtacgg cggccgtcag accttcttgc agaccacgta
     1141 ctggttgttg ccgctctcga cgtagttcgg ggcagcgtag gtcgggcccg cctcgatgtc
     1201 caggtttgga gccgtctgga gatagccgta catctggccg gtctgcgacc gctggtcgtc
     1261 gacctgggtg tcgacggcgc tcgcgatctt gtccgggatg ttcgccgagc aaaggacgag
     1321 acccgcgagc cggggctgcc cggcggcgat gcccggcgcg ccgttctgca cgccgacgaa
     1381 gccgcccagg gcgttgaccg gtggcgtcca ggacggcggt cccgccgtcg cgccggccac
     1441 gaaaccggcc aggcgcaggt gccaccagaa caggttcgac tcctcgttgg cggccggggc
     1501 ggtgcccgtg agcgccgccg gtgcggcctg gtaaaagccg gcgatgacgc cgttgccgtt
     1561 gccgttgcgc gagccgcctg gcgggtttgg atccgcccag cggacgttcg tctggttgtc
     1621 gtcgccggga acctgcttgt agcggtcctg gtagccgaag tacgcggccg tgatgccgtt
     1681 gaagtcgttc acgacgttct tgatccgggc ctgagtgatc atctcctggc ccttcaggat
     1741 gccgccaagc agcaacccga tgatgaccag cacgatcgcg atctcgacca gggtgaaacc
     1801 cgcctgcatg cgctttgatt gcattgtctg tctccttaca gcccggcaac cgcgcccggc
     1861 tggctagtgc ggagacaaga gcaaactcga tgccagccct ttcccttgat t
//
LOCUS       DBNHXO010000260         9609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1461039, whole genome shotgun sequence.
ACCESSION   DBNHXO010000260 DBNHXO010000000
VERSION     DBNHXO010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9609
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1461039"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="AC162A_05330"
     CDS             complement(<1..605)
                     /locus_tag="AC162A_05330"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HIR5010296.1"
                     /translation="MTTDDAAAPTSAQPTGAGAWVILPTYNEADNLPGISAAILESLP
                     GAVLLVVDDGSPDGTGALADGLAQADARVRVKHRAAKQGLGRAYLDGFGVALTGGATM
                     VLQMDADWSHDPLALPALIAPIVDGKADLVIGSRYTKGGGVEDWGIIRRVISRGGSTF
                     ARIVLNLKPHDLTGGFKAWRATTLASVPFDGVRAGGYVFQI"
     gene            complement(672..4484)
                     /locus_tag="AC162A_05335"
     CDS             complement(672..4484)
                     /locus_tag="AC162A_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HIR5010297.1"
                     /translation="MPRPRIALDVARPALSAGLALVLAAGALPSPVGAAAPTTPVFET
                     LPAAAAQDVPPTRADEAPPAIDDAVTPEQHPSIAYEEAMAHADDVIAFEPGGRVKVGF
                     RPRADDDWPVDNRAPTSLPAGRATGHQMAASLQGSQWATVRGASAPSTVDAAGDGAET
                     SEAPVDAPGGAPSIGATASSYVLPDARPEADLAAAPGLRRQVFGFLPYWELSGASSKI
                     NHQVLSTIAYFSVGADRDGNLRKKGGDGRTTTGWGGWTSSNMTSVISNAHRHGTRVVL
                     TLSVFAWTTAQANTQRAILGSKAARVRLARQAVAAVRDRGADGVNLDFEPLASGYADE
                     FVALLRTFRTELNKVRKGYQLTYDTTGYIGNYPLEASVGKGAADAIFVMGYDYRTSSS
                     GRAGSIDPLSGPAYDLADTVRAYTARVSPSRIILGLPWYGRAWSTEDKDPRSKTLSGA
                     KYGYSAAVNYENVVGLVAKHGRKWDALERSPYVVYRRENCTSTYGCVTSWRQVYFDDA
                     ASLKQRYAIVNDYGLRGAGMWALGYDGGHAELNRALSESFRVDKSAPQAGIRMLASAQ
                     GDEGFVVSWTARDTSTIASYDVQVSVDGGPWAAWRTRTRARSEVWLGSDGHGYAFRVR
                     AVDSKGNAGSWNVGSRWDGSPRLEDGGFGRVVADGLAYRSGPDASAARLGTLPAGTIV
                     AITRGPVSSDGYSWYEVTEPIREWSPVSFVERGVWIAARSSSDTMVKPYRAPNSTTVN
                     AGIRGLNFGSGSASGVGSGPTARAVRAFSPNGDGWEDAVRLRWTNTVALDSLTLNVYR
                     TNGTLVGSRTVPDVRTGAQTWDWNGAAGGSTVRDGRYVLQLVGKAGARTFRAPSIRPV
                     TGAQVGAFAVTVDTVRPKIKSASASSTLISPNGDDVRESVRFALTSTGSTRWALQIAS
                     AGAGTVGTIDGSGSSFGFTWRGEKEGGGRAPDGRYTTTLSAIDDAGNRARRSFTVTLD
                     TTPPSITTAATPGAFSPDGDGALESTRLSWTANGRATGTVRILKGSTEIRRWPINHAS
                     SWATTWDGRNAAGKRVADGRYEMRVSLVDPGGNRRTVDRALVVDRTGGFLRWSRSFFP
                     QDADSLAPTSALTWRLARDARTSLRLYDASGTLVRTVWTGKAQHAGDRKWTWNGRLAG
                     GSFAPQGRYEARLTVASTLGTVVLSRTVWASAFSVTPSATKVRPGQTLKVTFTTVEPL
                     TSRPTVAFRQPGRSAATVTATKRSDGSYTASFKVRSGGSGTGSIRVSAKDAGGGLNRT
                     TVAIRIAS"
     gene            complement(4710..5570)
                     /locus_tag="AC162A_05340"
     CDS             complement(4710..5570)
                     /locus_tag="AC162A_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010298.1"
                     /translation="MRKLAVLAAALLWIALLGQAAMPVAAAEGAKVAIIVGATHGATA
                     SYRRNADEIYAEAIKYTPNVVRVYSPNATWKRVKAAVNGASIIVYLGHGNGWPSPYTF
                     DPLFSTKNGFGLNKDLNHNGRRSDNELKYYGEPRIETLNPAPNAVVLLFHLCYASGNS
                     EPGRKAPSLSTAKQRVDNYASAFLRAGASAVIANGHSHDPYYIAALFTTIQTIDAYWR
                     GAPDFHDRATSFASSRSRGATYQLDPESAGRYYRSIAGDLEVTTTEVTGAPFAPPPDP
                     DPTPEPPPVP"
     gene            complement(5853..6782)
                     /locus_tag="AC162A_05345"
     CDS             complement(5853..6782)
                     /locus_tag="AC162A_05345"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009226 - nucleotide-sugar biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5010299.1"
                     /translation="MRVAVTGAQGRLGRALIEALENAPFTGTRGPIAWSRPELDLDTL
                     TVASVAALIERDRPEVLIHAAAWTDVDGCARDPGLAMRRNAVAAGLIADACASDGVHL
                     AVVSTNEVFDGARTDGLGYAPADPPHPVNAYGESKLEAEGLTTRAMGQAGGTPVAIVR
                     TAWLHGPPGNDFPAKIAGAALRAKAAGEPLRVVGDEIGTPTYTPDVADAIVELIWENA
                     LFDEAAPSTIHHLVNAGRASRADWAREVLRAASIDVAIEEVPASTWQRASTPPLWAVL
                     EPTPLPSGEPMRDWRLAFADAVPALNRELRSAR"
     gene            complement(6787..7884)
                     /gene="rfbB"
                     /locus_tag="AC162A_05350"
     CDS             complement(6787..7884)
                     /gene="rfbB"
                     /locus_tag="AC162A_05350"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR5010300.1"
                     /translation="MTTEPGPGAHLLITGGAGFIGSELVRRILGRRDGTRVTVLDKLT
                     YAGNRANLAAVEADPEQAARFTFVQGDIADPDVVGPLVEGAGAVVNVAAETHVDRSIL
                     DPEAFLRTGVIGVHVLLEAVRAETDRAQAGGRAHAPRLLQVSTDEVYGEVADGRSVET
                     DALEPRSPYAAAKAAGELLVRAYQVTFGIDAVITRGSNTYGPHQHPEKLIPLFVTNAL
                     AGEPLPMYGDGMQVRDWLHVSDHAAGIEFVLRRGKAGEAYNLAGENERPNREVIGQLL
                     AATGRDWSLVRSVPDRPGHDRRYAMDGSKATALGWRALVAFEDGLPETVAWYREHGDW
                     VAAARSGDWDAYYQRQYGDRLAAGQAVIEAG"
     gene            complement(7881..8648)
                     /locus_tag="AC162A_05355"
     CDS             complement(7881..8648)
                     /locus_tag="AC162A_05355"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018887670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotidyltransferase"
                     /protein_id="HIR5010301.1"
                     /translation="MKGLVLAGGTATRLFPLTIVTNKHLLPIYDRPMIYYPLDTLAGM
                     GIREVMVIVGGKSVGDIVELLGDGRHFGLDLTYRYQRGALGIAHAIGLARDFIGGDSF
                     CVVLGDNVLRGDPLAETARTFDDGPWGAGTLLYRVADPQRFGVAELDAAGNVIGFEEK
                     PARPKSDLIPIGVYFLRPDAFDVIETLAPSGRGEFEITDVLNHYIPQGGLFTRVYEGH
                     WADAGTAPSLLRAAELAESDHDAGKLEPPPAHPDDPR"
     gene            9002..>9609
                     /locus_tag="AC162A_05360"
     CDS             9002..>9609
                     /locus_tag="AC162A_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5010302.1"
                     /translation="MDARRLASRVADELRQGRVAAGISQSTAARAAGISRSQLGRLER
                     AETSAPDLDQICRAARALGLQARIRLYPVGTPVRDAAHLALLKRFELRLGAPLTLRRE
                     VPLPIEGDRRAWDAMIEGSGRPFFSEAETHIHDVQAFERTVELKQRDDPRATVIILVA
                     TRSEHNRRVIGEHREALRGLFPLDGGAVLRALSAGRSPGAGG"
BASE COUNT         1296 a         3328 c         3584 g         1401 t
ORIGIN      
        1 tcgatctgga acacgtagcc acccgcgcgg acgccgtcga acggcacgct cgcgagggtc
       61 gtcgcccgcc acgccttgaa gccgccggtg aggtcgtgcg gcttgaggtt gaggacgatc
      121 cgcgcgaacg tcgacccgcc gcgcgagatg acgcggcgga tgatgcccca gtcctcaaca
      181 ccgccgccct tcgtgtagcg cgacccgatc accagatcgg ccttgccatc cacgatcggc
      241 gcgatcagcg ccggcagggc cagcgggtcg tgcgaccagt cggcgtccat ctggaggacc
      301 atcgtggcgc cgccggtgag ggccacgccg aacccgtcga ggtaggctcg gccgaggccc
      361 tgcttggcgg cccgatgctt gacgcggacg cgtgcatcgg cctgggcgag gccgtccgca
      421 agcgccccgg tcccgtcggg cgagccgtcg tccacgacca ggagcacggc ccccggcaac
      481 gactcgagga tcgccgcact gatcccgggc aggttgtccg cctcgttgta ggtcggcagg
      541 atgacccagg cgccggcccc ggtgggctgc gcgctcgtcg gtgcggctgc gtcgtcggtc
      601 gtcatcgcgg ttcggtcgtc atgcggcgag agtgtacggg ccgacggggg cgcagggtcg
      661 aggtcggagg ctcacgacgc gatcctgatc gcgaccgtgg tgcggttgag cccgccgccc
      721 gcgtccttcg ccgagacccg gatcgagccg gtgcccgagc cgccgctccg caccttgaac
      781 gaggcggtgt acgagccgtc gctgcgcttc gtcgcagtca cggtggcggc ggaccggccg
      841 ggctggcgga acgcgacggt cggcctggac gtcagcggct cgacggtcgt aaaggtgacc
      901 ttcagcgtct ggccggggcg caccttcgtg gcggacggcg tcacggagaa cgcacttgcc
      961 cacacggtac gcgagaggac gaccgtgccc agggtcgagg cgaccgtcag ccgcgcttcg
     1021 tagcgaccct gcggcgcgaa cgacccgccc gcgaggcgtc cgttccaggt ccacttgcgg
     1081 tcgcccgcat gctgcgcctt gcccgtccag acggtccgga ccagcgttcc ggaagcgtcg
     1141 tacaggcgga gcgacgtcct ggcgtcacgc gccagccgcc aggtgagggc cgaggtggga
     1201 gcgaggctgt ccgcgtcctg ggggaagaag ctccgcgacc agcgcaggaa gccgccggtg
     1261 cggtcgacga ccaacgcgcg atcgaccgtt cgccggttgc cgcccgggtc gacgagcgag
     1321 acgcgcatct cgtaccgccc gtcggcgacc cgcttgccgg cggcgttgcg cccgtcccag
     1381 gtcgtcgccc agctcgaggc gtggttgatt ggccagcggc ggatctcggt gctccccttg
     1441 aggatccgga cggtgccggt ggcgcgcccg tttgccgtcc acgagaggcg cgtcgactcc
     1501 agcgccccgt cgccgtcagg cgagaacgcg cccggggtgg ccgcggtggt gatcgatggc
     1561 ggcgtggtgt cgagggtgac cgtgaacgag cgacgggcgc ggttgccggc gtcgtcgatc
     1621 gcggacaggg tcgtcgtgta gcggccgtcc ggcgcgcggc cgcctccctc cttctcgccg
     1681 cgccaggtga acccgaacga ggagccactg ccgtcgatcg tgccgacggt acccgcgccc
     1741 gccgaggcga tctggagggc ccagcgggtc gacccggtcg aggtgagcgc gaaccgcacg
     1801 gactcccgta cgtcgtcgcc gttgggcgag atcagcgtgc tcgaggcgga ggccgacttg
     1861 atcttcggtc ggacggtgtc gaccgtcacg gcgaacgcac cgacctgggc gccggtcacc
     1921 gggcggatcg atggcgcccg gaacgtgcgt gcgcccgcct tcccgaccag ctggaggaca
     1981 tagcgaccgt ccctgacggt cgacccgcca gcggcgccgt tccagtccca ggtctgcgcg
     2041 cccgtccgga cgtccgggac ggtccgcgac ccgaccagcg tcccgttcgt gcgatagacg
     2101 ttgagcgtca gcgagtcgag cgcgacggtg ttggtccagc ggaggcggac ggcatcctcc
     2161 cagccatcgc cgttgggcga gaaggcacgg acggccctcg cggtcggccc cgagcccacg
     2221 cccgacgccg accccgagcc gaagttgagg ccgcggatcc ccgcgttcac cgtcgtgctg
     2281 ttgggcgcgc ggtagggctt gaccatcgtg tcgctcgagg acctggcggc gatccacacg
     2341 ccacgctcga cgaacgagac gggtgaccac tcgcggatcg gctcggtgac ctcgtaccag
     2401 ctgtacccgt cggacgagac ggggccccgg gtgatggcga cgatcgtgcc ggccggcagc
     2461 gtgcccagcc tggcggccga ggcgtccggg ccgctgcgat acgcgagccc gtcggccacg
     2521 acccggccga agccgccgtc ctcgagcctg ggcgacccgt cccagcgcga gcccacgttc
     2581 cagctgcccg cgttgccctt gctgtcgacg gcacggacgc ggaacgcgta gccgtgcccg
     2641 tcggatccca gccacacctc ggaccgagcg cgcgtcctcg tgcgccacgc cgcccacggg
     2701 ccgccgtcga cggagacctg gacgtcgtac gaggcgatgg tgctggtgtc gcgagccgtc
     2761 cacgagacga cgaagccctc gtcgccctgg gcggaggcga gcatccggat gccggcctgg
     2821 ggcgcggact tgtcgacacg gaaggactcc gacagggccc ggttgagctc ggcgtggccg
     2881 ccgtcgtagc ccagcgccca catccccgcc ccgcgcagcc catagtcgtt gacgatcgcg
     2941 tagcgctgct tgagcgacgc cgcgtcgtcg aagtagacct gtcgccagct ggtcacgcag
     3001 ccataggtcg aggtgcagtt ctcgcgccgg tagacgacgt acgggctgcg ctcgagggcg
     3061 tcccacttgc gcccgtgctt ggccacgagg ccgacgacgt tctcgtagtt cacggcggcg
     3121 ctgtagccgt acttggcgcc cgaaagcgtc ttcgagcgtg ggtccttgtc ctccgtcgac
     3181 cacgcgcggc cgtaccacgg caggcccagg atgatccgcg acgggctcac ccgggcggtg
     3241 taggcacgaa cggtgtcggc gaggtcgtac gcggggccgg acagggggtc gatggagccc
     3301 gccctcccgg acgacgaggt ccggtagtcg tagcccatga cgaagatcgc gtccgccgcg
     3361 cccttgccga ccgacgcctc gagcgggtag ttcccgatgt agccggtggt gtcgtaggtc
     3421 agctggtagc ccttcctgac cttgttgagc tcggtccgga acgtcctgag cagcgcgacg
     3481 aactcgtcgg cgtagcccga cgcgagcggc tcgaagtcga ggttgacgcc gtcggcgccg
     3541 cggtcgcgga ccgccgcgac ggcctgcctc gcgagccgca ctcgggcggc cttgctgccg
     3601 aggatcgcgc gctgggtgtt cgcctgggcc gtcgtccacg cgaagacgct gagcgtgagc
     3661 acgacacggg tgccatggcg gtgcgcgttc gagatcacgg acgtcatgtt cgagctcgtc
     3721 cagccgcccc agccggtggt ggtgcggccg tcgccgccct tcttcctgag gttgccatcg
     3781 cggtccgcgc cgaccgagaa gtacgcgatc gtggacagca cctggtgatt gatcttgctc
     3841 gaggcaccgg agagctccca gtacgggagg aagccgaaca cctgccgcct gagcccgggc
     3901 gcggccgcga gatccgcctc gggtcgggcg tccggcagaa cgtacgacga ggcggtcgcg
     3961 ccgatcgacg gcgcgccacc cggcgcgtcg accggggcct cgctggtttc ggcgccatcg
     4021 ccggccgcgt ccacggtcga aggggcactg gcgccgcgta cggtggccca ctggctgccc
     4081 tggaggcttg cggccatctg gtggcccgtc gcgcggcccg cgggcagcga cgttggcgcg
     4141 cggttgtcga cgggccagtc atcgtccgca cggggccgga agccgacctt tacgcggcca
     4201 cccggctcga acgcgatcac gtcgtccgcg tgggccatgg cctcctcgta ggcgatcgag
     4261 gggtgctgct cgggcgtgac ggcgtcgtcg atggcgggcg gcgcctcgtc cgcacgggtc
     4321 ggggggacgt cctgggcggc cgcggccggg agcgtctcga acacgggggt cgtgggcgcg
     4381 gcggcgccga cgggcgacgg cagggcgccc gcggccagca cgagcgcgag gcccgccgaa
     4441 agggccgggc gggcgacgtc gagagcgatc cggggtcggg gcacggcctt cctctgcggc
     4501 gatgatcccc gcgtcgcccg ccggggacga cgtcgacgcg aggtgccggg ggcttcgatc
     4561 cgcggagcgg cgccacccgg cgaggcatgt ccacgctagg cccgggggcc tcgcgatccc
     4621 atcaccccaa ggtcccgatc cggtctggta cggaggggtc tggtggggtc gggaccggtt
     4681 cggtggggtc cctgtcgcgc tacgccgtgt caggggaccg gcggcggctc cggtgtcggg
     4741 tccggatcgg gcgggggcgc gaagggggcg cccgtcacct cggtcgtcgt gacctcgagg
     4801 tcgccggcga tcgagcggta gtaccgcccg gcgctctccg ggtccagctg gtaggtggca
     4861 ccgcgcgaac gcgacgacgc aaagctggtg gcccggtcgt ggaagtccgg cgcgccccgc
     4921 cagtaggcgt cgatggtctg gatggtcgtg aacagcgccg cgatgtagta cgggtcgtgg
     4981 ctgtgaccgt tcgcgatgac ggcgctggcg ccggcccgca ggaacgccga cgcatagttg
     5041 tcgacacgct gcttcgccgt cgacaggctg ggcgccttgc gacccggttc cgagttgccc
     5101 gaggcgtagc acaggtggaa cagcaggacg acggcgttcg gggcgggatt cagcgtctcg
     5161 atcctcggct cgccgtagta cttcagctcg ttgtcgctgc gcctgccgtt gtggttgagg
     5221 tccttgttga ggccgaagcc gttcttggtc gagaacaggg ggtcaaacgt gtaggggctc
     5281 ggccagccgt tgccgtggcc gagatagacg atgatcgagg ccccgttgac ggcggccttg
     5341 acccgcttcc aggtggcgtt ggggctgtag acgcggacca cgttcggcgt gtacttgatc
     5401 gcctcggcgt agatctcgtc ggcgttgcgc cgatagctcg cggtggcccc gtgggtcgcc
     5461 ccgacgatga tcgcgacctt ggcaccctcg gccgccgcga cgggcatggc cgcctgaccc
     5521 aggagggcga tccagaggag cgcggcggcg aggacagcga gcttccgcaa cgaacagctc
     5581 cgttgagcga tggacagcgc gcgcacccgg ggttgaccga ggcgcagatc agtgccggct
     5641 cagactcgcc gtgggcgagg gcggggccaa tcgcgggatg gtctggtccc ggctggccct
     5701 ttcgggctgg cgctgtcgag tgcggtgggg tctggtgcag tatgccgcgg tgcggacggg
     5761 caccgcactc cccaagcggt cgattcgggt tgcagtggcg ttgcatttcg ccgtggaggc
     5821 gccgggtggt gcgcccgcgc accagtcgcg ggtcagcgag cggagcgcag ctcccggttc
     5881 agggcgggga cggcgtcggc gaaggcgagg cgccagtcgc gcatgggctc gccggagggg
     5941 aggggcgtcg gctcgaggac cgcccacagg ggcggggtac tggcccgctg ccaggtggac
     6001 gcgggtacct cctcgatcgc gacgtcgatg ctcgccgctc gcaggacctc gcgtgcccag
     6061 tcggccctcg aagcccggcc tgcgttcacg aggtggtgga tcgtcgatgg ggcagcctcg
     6121 tcgaacaggg cgttctccca gatgagctcg acgatggcgt cggcgacgtc gggtgtgtag
     6181 gtcggcgtcc cgatctcgtc gccgaccacg cgaagcggct ccccggcggc cttcgccctg
     6241 agggctgcgc cggcgatctt ggccgggaag tcgttgccgg gcgggccgtg gagccacgcg
     6301 gtccggacga tcgctaccgg cgtgccgccc gcttgcccca tcgcgcgggt ggtgagcccc
     6361 tcggcctcga gcttgctctc gccgtacgcg ttgaccgggt gcggcgggtc ggcgggcgca
     6421 tacccgaggc cgtccgttcg ggcgccgtcg aacacctcgt tcgtggacac gaccgccagg
     6481 tgcacgccgt cgctcgcgca cgcatccgcg atcaggccgg ccgcgaccgc gttgcggcgc
     6541 atcgccagtc cggggtcccg cgcgcaaccg tcgacgtccg tccaggcggc ggcatggatc
     6601 agcacctccg gtcggtcgcg ctcgatgagc gccgcgacgg aagccacggt gagggtgtcg
     6661 aggtcgagtt cgggacgcga ccacgcgatc ggcccacgcg tgcccgtgaa cggcgcgttc
     6721 tccagcgcct cgatcagcgc gcggccgagg cggccctgtg cgccggtaac ggcgacccgc
     6781 atcgggtcag ccggcctcga tgaccgcctg cccggcggcg agtcggtctc cgtactggcg
     6841 ctggtagtag gcgtcccagt cgcctgatcg cgcagccgcg acccagtcgc cgtgctcgcg
     6901 gtaccaggcg accgtctccg gcagaccgtc ctcgaacgcc accagcgccc gccagcccag
     6961 tgccgtcgcc ttcgacccgt ccatcgcgta tcgccggtcg tgcccgggac gatcggggac
     7021 ggacctgacc agcgaccagt cgcggcccgt cgccgccagc agctggccga tgacctcgcg
     7081 gttcggccgc tcgttctcgc ccgcgaggtt gtaggcctcg cctgccttgc cgcggcgaag
     7141 cacgaactcg atccccgcgg cgtgatccga gacgtggagc cagtcgcgga cctgcatccc
     7201 gtcgccatac atcggcagtg gctcgccggc cagggcgttg gtgacgaaca gcgggatgag
     7261 cttctcgggg tgctgatgcg ggccgtacgt gttcgagccg cgggtgatga cggcgtcgat
     7321 cccgaacgtc acctgatacg cgcggaccag cagctcgccc gccgccttgg cggcggcgta
     7381 tgggcttcgc ggctcgaggg cgtccgtctc gacgctccgt ccgtccgcga cctcgccgta
     7441 gacctcgtcc gtgctgacct ggaggagcct cggggcgtgc gcccggccgc cggcctgcgc
     7501 ccggtccgtc tcagcgcgca cggcctccag cagcacgtga acgccgatga cgccggtccg
     7561 caggaaggcc tccgggtcga gaatcgagcg atcgacgtgg gtctcggcgg cgacgttgac
     7621 gacggccccg gcgccctcga cgagcggccc gacgacgtcg gggtccgcga tatcgccctg
     7681 gacgaaggtg aacctcgccg cctgctccgg gtccgcctcg accgccgcca ggttcgcccg
     7741 gttgccggcg taggtgagct tgtcgaggac ggtcacgcgg gtgccgtcgc ggcgaccgag
     7801 gatcctgcgc acgagctcgc tgccgatgaa tcccgcgccg ccggtgatca ggaggtgggc
     7861 gcccggaccg ggctcggtcg tcatcggggg tcgtcggggt gggccggcgg gggctccagc
     7921 ttgccggcgt cgtggtcgga ctccgcgagc tcggcggccc gcagcaggga cggggcggtc
     7981 ccggcatcgg cccagtgccc ttcgtagacc cgcgtgaaca ggccgccttg cgggatgtag
     8041 tggttgagga cgtcggtgat ctcgaactcg ccgcggccgg acggcgcgag cgtctcgatc
     8101 acgtcgaacg cgtccggacg gaggaagtag acgccgatcg ggatcaggtc cgacttcggc
     8161 cgggccggct tctcctcgaa cccgatgacg ttccccgcgg cgtcgagctc cgcgacgccg
     8221 aaacgctgcg ggtcggcgac gcggtacagg agggtgccgg cgccccacgg gccgtcgtcg
     8281 aaggtgcggg ccgtctccgc caggggatcg ccccgcagga cgttgtcgcc cagcacgacg
     8341 cagaagctgt cgccgccgat gaagtcacgg gcgaggccga tggcgtgggc gatccccagg
     8401 gcgccgcgct ggtaacggta ggtcaggtcg agcccgaagt gccgcccgtc gcccagcagc
     8461 tcgacgatgt cgccgacgct cttgccgccg acgatgacca tgacctcgcg gatccccatg
     8521 ccggccagcg tgtcgagcgg gtaatagatc atcggccggt cgtagatcgg cagcaggtgc
     8581 ttgttggtca cgatcgtgag cgggaacaac cgggtcgccg tcccgccggc aagcactagc
     8641 cctttcatcg gggagagcct agcagggggc gtcgggctca gatgacgtcc acggtaaggc
     8701 ccgacaaaac cctgcgcccc tcaagcctgc ccagaggacg ggccgctagg ggaaccacac
     8761 atggcgccgt gcgactgtct gctcactggg ggagcgggcc atcgcacgag tgggcggtcg
     8821 gcgccacgct tggaccgctg gccgagccag tggacgcgcg gcgcgatcag tgggccgcca
     8881 cgcggacgtg ggttggctgg ccgacgatgc gcgcctggcg cattggaata aggctgagcg
     8941 aagtctcaac agtcatcctg ccccaatgcc cacagtccag cgccgcgctc agctcggagc
     9001 catggatgcg cgccgacttg ccagccgtgt tgcggatgag ttgcgccaag gccgggtcgc
     9061 cgccgggatc agccagtcga cggccgcgcg cgcggcgggc atctcgaggt cccagctcgg
     9121 cagactggaa cgagccgaga cgagcgcgcc ggatctggac cagatctgtc gggcggcgcg
     9181 ggcactgggc ctccaggcga ggatccggct gtatccagtc ggcacgccgg ttcgcgacgc
     9241 agctcacctc gccctcctca aacgcttcga gctccggctc ggcgctccac tcaccctgcg
     9301 tcgcgaggtg cctctgccaa tcgaaggaga ccgacgcgcg tgggacgcga tgatcgaagg
     9361 cagtgggcga ccgttcttca gcgaggccga gactcacatc catgacgtgc aggccttcga
     9421 gcgcaccgtc gagctcaagc aacgggatga cccgcgagcg acggtgatca tcctcgtggc
     9481 gacgcgcagt gagcacaacc ggcgagtcat cggcgagcac cgggaagcgc ttcgcgggct
     9541 gttcccgctc gacggtggcg ccgttcttcg ggcgctgtcc gccggtcgat cgcccggcgc
     9601 tggcggaat
//
LOCUS       DBNHXO010000261         6784 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4039060, whole genome shotgun sequence.
ACCESSION   DBNHXO010000261 DBNHXO010000000
VERSION     DBNHXO010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6784)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6784
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4039060"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="AC162A_05365"
     CDS             complement(<1..403)
                     /locus_tag="AC162A_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010303.1"
                     /translation="MTKVVEFLPKVALFGVMVVVGFIVSSAVVQALQDRSPQSATTEA
                     PDYVLAGAPDTPGASAEPIAFNVEPSGSPETLESLAPADLTAAADTVLGAEPAVYKNP
                     SGFPRIPPISQFDGGPFQGANCTLTSGAMLAR"
     gene            complement(400..1902)
                     /locus_tag="AC162A_05370"
     CDS             complement(400..1902)
                     /locus_tag="AC162A_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010304.1"
                     /translation="MSALGAGVGKAVGGLGGPQIAAIVVAGGLLVGAIGGGLAGGGGP
                     GTQGTTQATLSIYPCPDAGPALATVGSGQKFLVTGKNADASWARIYYPLPGRTEAWVP
                     SGPLQVDGSLDAQPVVPCAPVVAGPGASVEPSSSLTAVGNNSPSPGPTAEPTITPSDS
                     GPTLARFDFGTRIAGGPQRYCERTPRSITVSVRATDANDVASVVLAYRKPGAADVATK
                     PMVRVGGSDTWRTSLATDANGITGPGSLRLYVSATDRDPTPHTSRLPARSIDVVACAN
                     TGPTLASLRAAPGTVSTNLAACQSKPKATAISVEATDVDGVDGATLHYRLPGDNAYRD
                     AKMTKNGNRWRTTVTPVDQRPNADGKASYYVTSDDDLGESSRSATRTFAVNRCNFPAA
                     IRYSDSTGTPFCPQRTTRFFYLANDRDGLTASSATFVYKYRLASGKMKTAKVKMSGQD
                     QGGNWFYTVDIVPTAKWALDGSTVTFHVQTKDRFGGISNGGNSKTTQSCP"
     gene            complement(1913..2974)
                     /locus_tag="AC162A_05375"
     CDS             complement(1913..2974)
                     /locus_tag="AC162A_05375"
                     /inference="COORDINATES: protein motif:HMM:NF051792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADase-type glycan-binding domain-containing
                     protein"
                     /protein_id="HIR5010305.1"
                     /translation="MTDDTRTICKRCGFTNIPGDQFCGSCGTFLEWDGVPAEGAPAVV
                     DAAPPPDPQPSAPVAAPPPDDQAGYGDLVRCPACGTANASSRTFCQSCGATLAAAERV
                     EAASPEQIAQAVAWTSAPGTKAGPTTKTAGGDASSRSRGIPSWIVWVGVIGVLVGVAI
                     VLGGLALRGENPGTGATGSVRPSGPASSDGPGSSGPGDSTAPGTAVPIPLSEAEASST
                     VAAKFQANKAIDGDPDTSWQEGKGQEKGEWIEVTFDSSRADTLLIRNGYGASQALFKG
                     NRRLKDVEVSVNGGAPIAVRLKDVIKEQEVDLGGVSGATSVRITIVSTYPGQKTSVAD
                     TPFDDAAVSEISVLGVPGG"
     gene            complement(2981..5038)
                     /locus_tag="AC162A_05380"
     CDS             complement(2981..5038)
                     /locus_tag="AC162A_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5010306.1"
                     /translation="MARRVAAVALLLALGSAVPATPAVRAVAGVQAPPAPRPVVSAPV
                     PTPPGRTLLASKSAGGGFPNALSWTPSISANGRYVAYASTATNIVRAGPGAPAAGVFL
                     RDMSNGRTIRLPIPASLTRGGRATEPSISADGSVVAFTYQEPATDDGSVVLAWDRSTG
                     RSQVVSRTNAGGIVQGSSQPSVSATGRFVAYTSTRQNLVGGPGGDPDVFRYDRDRQRT
                     VVVSVGQVSPLDPGRDTAPSISANGNVVAFQSDGGDQLIPANTGPGDQVFVRDIAAER
                     TRQVSVALDGPPNGPSVTPSISADGNVIAFTSAASDLASIDDNVVPDVFVRDMRAGAT
                     TMVSVTPDGRAPTARSEGPSISADGRMVAFQSEALDIIGLGASVATAHLAAAAAKRTE
                     VFERDLVANETVLVSVGRNGRPGGLVSAQPVVGGNGRFVAFASTSPRLVPRDTLGVTD
                     VFLRDMPPVPRLNPSSIDLGTLDVGIASAPGAAILGNSGWGPLSVGRAAVTGPERLDF
                     DVVADACDGRVLHRTEKCTVTVTFTPRGGGRRDATLAIPDGYTGSPRTARLRGSGVRA
                     PVNRPRFEISPEVGAPGLVVVATGSDFPPNSLVRLSWSRGTTPTMPAVRAGADGRFTA
                     QVLVFHNDLTGPRDLRADPVTPGAFPRATARLLVTRPSVTPPGFLMIRRFIDLPLVLL
                     MRG"
     gene            complement(5044..6294)
                     /locus_tag="AC162A_05385"
     CDS             complement(5044..6294)
                     /locus_tag="AC162A_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COG1470 family protein"
                     /protein_id="HIR5010307.1"
                     /translation="MGASVTLVTPALAVEPGQETTAEVRVRNTGSVVDEFSLDVIGDS
                     AGWAVAEPAVISLVPGAEATAKLVFRPPRAASTPGGLVPYGIRARSKEDPAGSAVEEG
                     SINVGTFLDPFAELAPRTSRGSRTGTHDLAIDNRGNARLNAEVEGTDADRNLKFDINP
                     PGVVVEPGMAGFAKVRVSPVKRFWRGQPKTRPFQLFVKPEGGVPITIDGTLLQESVLP
                     SWFLKALLALIVLLIALVLIWLLLLKPTIQTAASDAVASPLASLKADVNDALADAGLA
                     TMGPEPSAGDGGPTPPAPTAPAPSDGSGGPPSPTSTPGVVIPGLGNPVDGRLDKANPS
                     MTAAGTLFVTDLVFSNPNGATGSVDIKRDDTSLLHLRLENFRDYDLHFVTPIVIAPGQ
                     SLVLSPACETACDPAVFYSGYLRP"
     gene            6507..>6784
                     /locus_tag="AC162A_05390"
     CDS             6507..>6784
                     /locus_tag="AC162A_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010308.1"
                     /translation="MVAELMPIKAPIPVAVRASDPLSRAGIASQLRRAQGFVVVADDA
                     TPEDSVALVVAEEVAPEVLAEMRALRGRGVRRIVLLVSRVDDRSLLAAA"
BASE COUNT          896 a         2369 c         2562 g          957 t
ORIGIN      
        1 ggcgggccag catcgcgccc gaggtgagcg tgcaattggc gccctggaac ggtccgccgt
       61 cgaactgcga gatcggcggg atgcgcggga atcccgacgg gttcttgtag acagccggct
      121 cggcgccgag gacggtgtcg gcggcggcgg tcaggtcggc aggggcgagc gactcgaggg
      181 tctcgggcga gcccgatggc tcgacgttga aggcgatggg ctcggccgag gcgccgggcg
      241 tgtcgggggc gccggcgagg acgtagtcgg gcgcctcggt cgtcgccgac tggggcgagc
      301 ggtcctggag ggcctggacg acggcagagc tgacgatgaa gcccacgacg accatgacgc
      361 cgaacagcgc gaccttgggc aggaactcga cgaccttggt cacgggcagc tctgcgtcgt
      421 cttggagttg ccgccgttgg agatgccgcc gaagcggtcc ttcgtctgca cgtggaacgt
      481 cacggtggag ccgtcgagcg cccacttcgc ggtcggcacg atgtcgaccg tgtagaacca
      541 gttgccgccc tggtcctggc ccgacatctt gaccttcgcc gtcttcatct tgccgctggc
      601 gagccggtac ttgtagacga acgtcgcgct cgaggcggtg agcccgtccc gatcgttggc
      661 gaggtagaag aagcgcgtcg tgcgctgcgg gcagaacggc gtcccggtgc tgtcggagta
      721 gcggatcgcg gcggggaagt tgcagcggtt gaccgcgaac gtccgcgttg ccgacctcga
      781 tgactcgccg aggtcgtcgt cgctcgtgac gtagtacgac gccttgccgt ccgcgttcgg
      841 ccgctggtcg acaggcgtca cggtcgtccg ccaccggttc ccgttcttcg tcatcttggc
      901 gtcgcgatac gcgttgtcgc ccggcagccg gtagtggagc gtcgcgccgt ccacgccgtc
      961 gacgtcggtc gcttcaacgg agatcgcggt cgccttcggc ttggactggc acgcggcgag
     1021 gttcgtcgac acggttcccg gcgcggcccg cagcgaggcc agcgtgggac cggtgtttgc
     1081 gcacgcgacg acatcgatcg agcgcgccgg gagccgcgag gtgtgcggcg tcgggtcgcg
     1141 gtccgtggcg ctcacgtaga ggcgcaggct gccaggcccg gtgatgccgt ttgcgtcggt
     1201 ggccagggac gtgcgccagg tgtcgctccc gccgacgcgc accatcggct tggtcgcaac
     1261 gtcggcggcg cccggcttgc ggtaggccag gacgacgctg gcgacgtcgt tggcatccgt
     1321 cgcccgcacg ctgacggtga tcgagcgcgg cgtccgctcg cagtagcgct gcggaccgcc
     1381 ggcgatcctc gtgccgaagt cgaagcgggc gagggtcggg ccgctgtcgg acggcgtgat
     1441 ggtcggctcc gccgtagggc ccggcgaggg actgttgttc ccgacggccg tgaggctgga
     1501 gctcggctcc accgacgcgc cggggccggc gacgaccggc gcgcagggca cgaccggctg
     1561 cgcatcgagc gagccgtcga cctggagcgg gcccgacgga acccaggcct ccgtacgtcc
     1621 gggcagtggg tagtagatgc gcgcccagct ggcgtcggcg ttcttgccgg tgaccaggaa
     1681 cttctggccc gagccgacgg tcgctagggc cggccccgcg tccggacagg ggtagatcga
     1741 caacgtcgcc tgggtcgtcc cctgcgtgcc gggtccgccg ccgccggcca gcccgccacc
     1801 gatggcgccg accaggaggc cgcccgcgac cacgatcgcg gcgatctgcg gcccgccgag
     1861 tcccccgacg gccttgccga ctccggcccc caacgcgctc acgcggctcc cctcagccgc
     1921 ccggcacgcc gagcacggag atctcgctca ccgcggcatc gtcgaagggc gtgtcggcga
     1981 ccgaggtctt ctgccccggg taggtcgaca cgatcgtgat ccggactgat gtcgcgcccg
     2041 acacgccgcc gaggtcgacc tcctgctcct tgatcacgtc cttgagccgg accgcgatgg
     2101 gcgccccgcc gttgaccgag acctcgacgt ccttgagccg ccggttgccc ttgaacagcg
     2161 cctgcgaggc gccgtagccg ttccggatga gcaacgtgtc ggcgcgggac gaatcgaagg
     2221 tcacctcgat ccattcgccc ttctcctggc ccttgccctc ctgccagctg gtgtccgggt
     2281 caccgtcgat ggccttgttc gcctggaact tcgccgccac ggtcgacgac gcctcggcct
     2341 ccgacaacgg gatgggtacg gccgtgccgg gcgcggtgga gtcgcccggg ccgctcgacc
     2401 ccggcccatc gctggacgcc ggccccgatg gacgcacgct ccccgtcgcg ccggtgcccg
     2461 ggttctcgcc ccgcagcgcc aggccaccga gcacgatcgc gaccccgacg agcacgccga
     2521 tcacgcccac ccagacgatc cagctcggga tgccgcgaga cctcgacgac gcatccccgc
     2581 cggctgtttt cgtggtcggg ccggccttgg tgccgggcgc cgacgtccag gcgaccgcct
     2641 gcgcgatctg ctccggggac gccgcctcga cgcgctccgc ggccgccagg gtggcgccgc
     2701 acgactggca gaacgtgcga ctcgacgcgt tggccgtccc gcaggcgggg cagcggacga
     2761 ggtcgccgta gcccgcctgg tcgtcgggcg ggggcgccgc cacgggggcg ctcggctgcg
     2821 ggtcgggcgg cggggcggcg tcgacgacgg cgggagcgcc ctccgcgggc accccgtccc
     2881 actcgaggaa cgtgccgcag gagccgcaga actgatcgcc cgggatgttc gtgaatccgc
     2941 agcgcttgca gatcgtccgg gtgtcgtcgg tcattcggcg tcagcctcgc atcaggagca
     3001 cgagcgggag gtcgatgaag cggcggatca tgaggaatcc cggcggcgtg accgacgggc
     3061 gtgtcaccag gagccgtgcc gtcgcccggg gaaacgcccc cggtgtgacg gggtccgcac
     3121 gcaggtcgcg cgggcccgtc aggtcattgt ggaacaccag cacctgcgcg gtgaaccggc
     3181 cgtccgcgcc ggctcgtacg gccggcatcg tcggtgtcgt cccgcggctc catgacaggc
     3241 ggaccaggct gttgggcggg aagtcggatc cggtcgcgac cacgaccagc cccggcgccc
     3301 cgacctcggg gctgatctcg aaccgcggcc ggttgaccgg cgccctgacc ccggagccgc
     3361 gcaggcgggc ggtccggggc gaacctgtgt acccgtcggg gatcgcgagc gtcgcgtccc
     3421 gccggccgcc gcccctcggg gtgaaggtca cggtcaccgt gcatttctcc gtgcgatgga
     3481 gcacccggcc gtcgcaggca tcggcgacaa cgtcgaagtc gagccgctcc gggccggtga
     3541 ccgcggcgcg accgacgctc aatgggcccc agccgctgtt gccgaggatc gctgcccccg
     3601 gcgccgacgc gatgccgacg tcgagcgtgc cgaggtcgat cgaggacggg ttgaggcgcg
     3661 gcacgggcgg catgtcgcgc aggaacacgt cggtcacgcc cagggtgtcc cggggcacga
     3721 ggcggggcga tgtcgaggcg aaggcgacga accggccgtt cccgccgacg acgggctgcg
     3781 cgctcaccag gccgcccggc cggccgttcc ggccgaccga cacgagcacc gtctcgttcg
     3841 cgacgaggtc ccgttcgaac acctcggtcc gcttcgccgc cgcggcggcc aggtgcgcgg
     3901 tcgcgacgct cgcgccgagg ccgatgatgt cgagggcctc ggactggaag gcgaccatcc
     3961 ggccgtccgc cgagatcgac ggcccctcgc tgcgggcggt cggggcgcgg ccgtcgggcg
     4021 tgaccgagac catcgtggtc gcgcccgcac gcatgtcccg cacgaacaca tcgggcacga
     4081 cgttgtcgtc gatcgacgcg aggtcagacg cggccgaggt gaaggcgatc acgttgccgt
     4141 cggccgagat cgatggcgtg accgatggcc cattcggtgg gccgtcgagg gcgacgctga
     4201 cctgcctcgt ccgctcggcc gcgatgtcgc gcacgaagac ctggtcgccg ggcccggtgt
     4261 tggcggggat cagctggtcc ccaccgtccg actggaacgc cacgacgttg ccgttggccg
     4321 agatcgacgg tgcagtgtcg cggcccggat cgagcgggga gacctgccca accgacacga
     4381 ccacggtccg ttgccgatcc cggtcgtagc ggaacacgtc cgggtcgccg cccggcccgc
     4441 cgacgaggtt ctgccgcgtg gacgtgtagg cgacgaagcg cccggtcgcc gacaccgatg
     4501 gctgactgct gccctggacg atcccgcccg cgttggtccg cgagaccacc tgggagcgcc
     4561 cggtcgaccg gtcccaggcg agcacgaccg agccgtcgtc cgtcgccggc tcctggtagg
     4621 tgaacgcgac gacggagccg tcggccgaga tcgagggctc ggtcgcccgg ccgccgcggg
     4681 tgaggctagc ggggatcggg agccggatgg tccgcccgtt gctcatgtcc cgcaggaaca
     4741 cgccggccgc gggcgcgccg gggcctgccc gcacgatgtt ggtcgcggtc gacgcgtagg
     4801 cgacatagcg gccgttggcc gagatcgacg gcgtccagga cagcgcattc gggaaaccgc
     4861 ccccggcact cttcgaggcc aggagggtgc gtccgggcgg cgtcggcacg ggcgccgaga
     4921 ccaccggtcg cggcgcgggc ggcgcctgca cgccggcgac ggcacgaacc gcgggcgtgg
     4981 ccggcacggc cgatcccagc gccagcagca gcgcgacggc ggcaacgcgg cgcgccatgg
     5041 gcctcagggc cgcaggtacc ccgagtagaa gacggccgga tcgcaggcgg tctcacaggc
     5101 ggggctcagg acgagcgact gccccggcgc gatcacgatc ggggtcacga agtggaggtc
     5161 gtagtcgcgg aagttctcga gccgcaggtg cagcagggac gtgtcgtcgc gcttgatgtc
     5221 gacgctgccc gtagccccgt tcgggttgga gaacacgagg tccgtcacga acagggtgcc
     5281 ggcggcggtc atgctcggat tggccttgtc gaggcgcccg tcgacggggt tgccgagccc
     5341 ggggatcacg acgcccggcg tgctcgtcgg cgacggcgga ccgccgctgc cgtcgctggg
     5401 tgcgggggcc gtcggggcgg gcggcgtggg tccgccgtcg ccggcgctcg gctcggggcc
     5461 catcgtcgcc aggccggcgt cggcaagcgc gtcgttgacg tccgccttga ggctcgcgag
     5521 cggcgaggcc acggcgtcgc tggccgcggt ctggatcgtc ggcttgagca gcagcagcca
     5581 gatgagcacg agggcgatca gcagcacgat cagcgccagc agggccttga ggaaccacga
     5641 cgggagcacc gactcctgga gcagcgtgcc gtcgatcgtg atcgggacgc cgccctcggg
     5701 cttcacgaac agctggaatg ggcgagtctt gggctggccg cgccagaacc gcttgaccgg
     5761 gctgacccgc accttcgcga agccggccat gccgggctcg accacgacac ccggcgggtt
     5821 gatgtcgaac ttcaggttcc gatcggcgtc ggtgccctcg acctcggcgt tcagccgcgc
     5881 gttgccgcgg ttgtcgatcg cgaggtcgtg ggttccggtg cgcgagccgc gcgaggtgcg
     5941 cggcgcgagc tcggcgaagg ggtcgaggaa ggtcccgacg ttgatcgagc cctcttccac
     6001 cgctgacccc gccgggtcct ccttcgagcg cgcccggatc ccgtacggga cgaggccgcc
     6061 gggcgtcgaa gcggcgcgcg gcggccggaa gacgagcttg gcggtggctt cggcgccggg
     6121 gaccagggag atgaccgcgg gctccgcgac cgcccagccg gcggagtcgc cgatcacgtc
     6181 aagcgagaac tcgtcgacca cgctgcccgt attccggacc cgcacctcgg cggtggtttc
     6241 ctgtcccggc tcgacggcca gggcaggtgt gaccagcgtg acggatgcac ccacgggcgt
     6301 gagtgtagcc gcgcaatcgc cgccggaaca ggaccccatc gcatggcccg atagtccttt
     6361 cgggcgtcct ttcggggcat gtcgggctgc ccgttaagtg cacgttgact ggccggacac
     6421 ccacgcgcgg tagggtccgc cgaggtcttc cggctgccgc ctccttccct ggttgccgtt
     6481 ggtggggacc catggggagt gcacgaatgg tcgccgaact gatgcccatc aaggccccga
     6541 tcccggtcgc cgtccgggcg tccgatccgc tctcgagggc cggcatcgcg agccagctgc
     6601 ggcgcgcgca gggcttcgtg gtggtggccg acgacgcgac gcccgaggac tccgtggcgc
     6661 tggtcgtcgc cgaggaggtg gcgcccgagg tgctggcgga gatgcgcgcg ctgcggggcc
     6721 gcggcgtgcg gcggatcgtg ctgctcgtgt cgcgcgtgga cgatcgctcg ctcctcgcgg
     6781 ccgc
//
LOCUS       DBNHXO010000262         2073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1065496, whole genome shotgun sequence.
ACCESSION   DBNHXO010000262 DBNHXO010000000
VERSION     DBNHXO010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1065496"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="AC162A_05395"
     CDS             <1..291
                     /locus_tag="AC162A_05395"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181550.1"
                     /note="catalyzes the formation of L-aspartate
                     4-semialdehyde from L-homoserine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR5010309.1"
                     /translation="VPTAVPAGSPFGRCDGVLNRVEVDADPVGRVAFEGPGAGGPATA
                     SAVLGDLIAIARGLGSTWAGSAPAIDRSAIAVRPVASECVAAPSGACYPSAD"
     gene            299..1381
                     /gene="thrC"
                     /locus_tag="AC162A_05400"
     CDS             299..1381
                     /gene="thrC"
                     /locus_tag="AC162A_05400"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR5010310.1"
                     /translation="MISPNQAARPQLVERYREFLPVGDATPVVTLGEGATPLLRARHL
                     GESLGLRNLHLKIEGQNPTGSFKDRGMVVAVAKALEEGARSVICASTGNTSASAAAYA
                     AAAGLECVVVLPAGKIAVGKLLQALVFGARVISVRGNFDDALRVVRALSEQDEHPITL
                     VNSVNPFRLEGQKTAAFEVCDDLGRAPDVLAIPVGNAGNISAYWRGFREYRDAGRSDT
                     VPRMWGFQAAGAAPLVTGVPVDHPETVATAIRIGNPASWKLAIAARDESEGRIDAVTD
                     DEILAAHRDIARLAGVFCEPSSAASVAGVAKAARAGEIDPDATIVCVLTGNGLKDPTT
                     AEAGLSVDVIEAEPSIADVSRALGWW"
     gene            1375..>2073
                     /locus_tag="AC162A_05405"
     CDS             1375..>2073
                     /locus_tag="AC162A_05405"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR5010311.1"
                     /translation="MVARGDWSALVGRALTVEVPASSANLGAGYDCLGLALDLVNHVS
                     VEAVEGDGSIGLAVRGEGEGELPASRSNRFVAGLEAALEAAVGPRPASIGWRIAMDNA
                     IPLSRGLGSSAAATVAGVVAGAELAVRAGAGRPDDEALLQIASTIESHPDNAAAVLLG
                     GFVVSAQLADRVAAIRFDAPAGLRCVLYIPDRRLATEDMRRVLPEQVPLRHAVENLGR
                     VAIGVAGIASGRYET"
BASE COUNT          249 a          742 c          796 g          286 t
ORIGIN      
        1 gtgccgacgg cggtcccggc cgggtcgccg ttcgggcgct gcgacggcgt gctgaaccgg
       61 gtcgaggtgg acgcggaccc ggtcggacgc gtcgcgttcg agggtccggg tgcgggtgga
      121 ccggcgaccg cctcggccgt gttgggcgac ctgatcgcga tcgcgcgcgg cctgggctcg
      181 acatgggcgg gctccgcacc cgcgatcgat cgatcggcca tcgcggtgcg cccggtcgcg
      241 tcagaatgcg tcgcggcgcc gtccggcgcc tgctatccca gcgcggactg aggctctcgt
      301 gatctcgccc aaccaggccg cccgccccca gctcgtcgag cgctaccgcg agttcctgcc
      361 cgtcggcgac gcgacgcccg tcgtcacgct cggcgagggc gcgacgccgc tgctgcgcgc
      421 ccgccacctc ggcgagtccc tcggcctgcg caacctccat ctcaagatcg agggccagaa
      481 cccgacgggc agcttcaagg accggggcat ggtcgtcgcg gtggccaagg cgctcgagga
      541 gggggcacgg agcgtcatct gcgcctcgac cggcaacaca tcggcgtccg cggccgccta
      601 tgccgccgcc gccgggctcg agtgcgtcgt cgtcctgccg gccggcaaga tcgccgtcgg
      661 caagctgctc caggccctcg tgttcggcgc ccgcgtgatc tcggtgcgcg gcaacttcga
      721 cgatgcgcta cgcgtggtgc gtgccctgtc cgagcaggac gagcacccga tcacgctcgt
      781 caactccgtg aacccgttcc gcctcgaggg ccagaagacg gccgcgttcg aggtgtgcga
      841 cgacctgggc cgggcgccgg acgtcctcgc gatcccggtc ggcaacgccg gcaacatcag
      901 cgcctactgg cggggcttcc gcgagtatcg cgacgccggc cgttccgaca ccgtgccccg
      961 gatgtggggc ttccaggccg ccggcgcggc gccgctcgtg acgggcgtgc ccgtcgacca
     1021 cccggagacc gtcgccaccg cgatccggat cggcaacccc gcgtcgtgga agctggcgat
     1081 cgcggcgcgc gacgagtcgg agggccggat cgacgccgtg accgacgacg agatcctcgc
     1141 ggcccacagg gacatcgcgc ggctcgcggg cgtgttctgc gagccctcgt ccgcggcctc
     1201 ggtggccggt gtggccaagg cagcgcgcgc gggggagatc gaccccgacg caacgatcgt
     1261 gtgcgtgctg accggcaacg gcctcaagga cccgaccaca gccgaggcgg ggctgagcgt
     1321 ggacgtcatc gaggcggaac cgagcatcgc cgacgtcagc cgggcgctcg gctggtggta
     1381 gcgcgcgggg actggtccgc gctcgtgggg cgcgcgctga ccgtcgaggt cccggcgtcg
     1441 tccgccaatc tcggcgccgg gtacgactgc ctggggcttg cgctcgatct cgtgaatcac
     1501 gtctcggtgg aggcggtgga gggcgacgga tcgatcgggc tcgcggtgcg cggcgaggga
     1561 gagggcgagc tgccggcttc ccggtcgaac cggttcgtgg cggggctcga ggccgcgctc
     1621 gaggcggcgg tcggcccgcg gccggcctcg atcggctggc ggatcgcgat ggacaacgcg
     1681 atcccgctgt cccggggcct gggctcgtcg gcggccgcga ccgtggccgg ggtcgtcgcc
     1741 ggcgccgagc tggcggtccg cgcgggcgcc ggcaggcccg atgacgaggc gctgctccag
     1801 atcgcgtcga cgatcgagtc ccatcccgac aacgcggcgg cggtcctgct gggcgggttc
     1861 gtggtctcgg cccagctcgc cgatcgggtg gcggcgatcc ggttcgacgc gccggcgggg
     1921 ctccgctgcg tgctgtacat ccctgaccgc cgcctcgcga ccgaggacat gcgccgcgtg
     1981 ctgcccgagc aggtgccgct ccggcatgcc gttgagaacc tgggacgggt ggcgatcggc
     2041 gtcgcgggga tcgcttcggg ccgctacgaa acg
//
LOCUS       DBNHXO010000263         8522 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2650487, whole genome shotgun sequence.
ACCESSION   DBNHXO010000263 DBNHXO010000000
VERSION     DBNHXO010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8522
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2650487"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="AC162A_05410"
     CDS             <1..497
                     /locus_tag="AC162A_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5010312.1"
                     /translation="LVVAEEVAPEVLAEMRALRGRGVRRIVLLVSRVDDRSLLAAAEA
                     GVTGVIRRSEATAGNLAAAIRSAAAGEGTLPPDLLGRLLRQVGQLQRQVLSPRGLTFS
                     GLTEREAAVLRLLADGYDTAEVGARLFYSERTVKNIIHDVTSRLELRNRAHAVAYAIR
                     EGLI"
     gene            complement(508..1236)
                     /locus_tag="AC162A_05415"
     CDS             complement(508..1236)
                     /locus_tag="AC162A_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010313.1"
                     /translation="MGEPSEALVRYRRTGGRPPSDDEALDVADDGSFTARRTVAGARG
                     GTFAGRLASGRVEALREAVDAVRDAHDVQVATPLDGATEVLEAGGHTTRTGSNERPTD
                     PWKALVETVRSMLDEDAVADPLAAIELTATSREAALTQAGPAPVEIDLGSVTLRVVRI
                     DTAGLVLGRWQARLQDAGDEDTAEPPPAWTSAAAGWRATLPFSHGLELAGGDWLQVWV
                     FATIRDAAGDGGERAARLVVAVPG"
     gene            complement(1236..3041)
                     /locus_tag="AC162A_05420"
     CDS             complement(1236..3041)
                     /locus_tag="AC162A_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR5010314.1"
                     /translation="MAGPGAQRVQHGDDRQPGERGGTADLAPGRDDAVDLGAIGAGPR
                     RLPVHAASSMVVMALQRSAGNAAVQSLLGPRPSLRLQRDDAPGATPVAPGASGAGAAA
                     APAPPARTALNPGDSSVEVGELQQALNALGAAVPPLRITALYGPETTAAVTAFQASHP
                     PLIPDGICGLPTWAKVDELAPRVSRQGRSVVEGPAEGEPRGTPLGGTIHPTVRRTSTG
                     PAVEELQQKLNTIPSDQVPTLLDVDGKFGRITRQAVIEFQQSRTPPLVADGVVGPLTW
                     SALDAVAGPVTVGRESFDWEQRAEGTITGGPSRFTWRLLDDKLQVTVNLRFTGSPSHP
                     RVAQWRTDITSVWNTFRFENPDDPGHPLDLEFVVGTGTPADASVAVVVTPAGTTPGRS
                     DSANWHTGDTDPGLAPHEFGHLIGLQDEYNRAPEQYAAVTGEQAPIGRVDAPLDAAGN
                     PVAPEVIAAEMRTAVTGAAAGRGAAARAVVTKYSLRQGAFSQRIALAYETANAGSMLR
                     EDFGPAGFVIVNDPAGTMGNDVAARIPGQFDTAGNEAVAVEPFLYSNRGIMGTMASLN
                     SPISAHDHPVAERHVRHFRDIVRRNRPGTWRTVRR"
     gene            complement(3045..3746)
                     /locus_tag="AC162A_05425"
     CDS             complement(3045..3746)
                     /locus_tag="AC162A_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019432504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eCIS core domain-containing protein"
                     /protein_id="HIR5010315.1"
                     /translation="MRDFELEAARGVPIAPERDLPTPATAESAPVAPGRSPALDAATL
                     MHLQRTAGNAGVVQMLAGEDEQVSPVHDVVGSGGGSALDAGTQSTMESAFGQSFDNVR
                     VHTDDQASQSAESVGANAYTVGSDIVFKSGQFDPGSASGQKTIAHELAHVVQQSRGPV
                     DGSEAAGGIRVSDPSDRFERAADATAEQVMASVSSSQPSASDAGALQREEDPLSAPAA
                     VQREALPEEEEEPGS"
     gene            3877..5523
                     /locus_tag="AC162A_05430"
     CDS             3877..5523
                     /locus_tag="AC162A_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005544 - calcium-dependent phospholipid
                     binding [Evidence IEA];
                     GO_function: GO:0048306 - calcium-dependent protein
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="annexin"
                     /protein_id="HIR5010316.1"
                     /translation="MQTRATRSPAGRSSAAAPATGGPRLTLEPAAGPLAASASTIEAS
                     VLDPAGVVDLQATAGNRATVEALQRTPAGGTAGPGLTVQRAVSPDQATQIARRLDDAM
                     RGWGTDEEAIYGALAGRTAADMDEIRKAYKSVANKDLDAELRDELNDSELARVTQSMA
                     PTADESTLTEGERATAATERAKVTAVHLKEAMAGWGTEEDEIFNALTGREPEEILAIQ
                     REYLALTGNHLDRDLQDELSGSDLRKALDLLQVQAAGTFTNEIDQHMTEGETTTVQGR
                     FEYVLQQRELTVRVPVDFKPDAGVTAPIATWQSQIDTVWNKFAAVETGGLKIPIRFGI
                     ENRSGAEKSIRVKDNKVPGTQSVEDRANAGMWFPLMDATTAPHEFGHLIGLQDEYQRT
                     HGDYKKITGQDITGPANASGKTAAEIAVDLHNALYLRDATQRAPTATTLLTAVGLIVG
                     GVPQQGDFAQEVQTAYNDKYDGWFSKSLVQAMRDKLPEGSKWTIQSVFSYASRSIMGN
                     PEVLGGVTPHDHAVEPRHLEEFVRIVRGAYPDLTWTSGPR"
     gene            5520..6200
                     /locus_tag="AC162A_05435"
     CDS             5520..6200
                     /locus_tag="AC162A_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010317.1"
                     /translation="MTLVTWRRLSSMPPPERELLDVEADGSFRLWRSIAPVVGRFGGS
                     LPAGVVSELEGLVSAVGSAAAPRPGSLPADASVEQLEVGGARSVFEAGAEADPPWGDL
                     LAACRRIADARTDAPVAAIALEVGLTDGRARIRLVQRGTGTLPVELASGRAKVAVWRG
                     MENPANALAGFDLGRVEAGPGWDAETTAELPVLEAGDKVTATVTFIVDDGGVFIPVAM
                     SATAVAPA"
     gene            complement(6208..6939)
                     /locus_tag="AC162A_05440"
     CDS             complement(6208..6939)
                     /locus_tag="AC162A_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010318.1"
                     /translation="MLPPPADRSATPDPLDEALAALDVRAKALSDAANDLFKAVARVV
                     ADAVLTAAAQIVEAQADHSLELGAKGLRQLRAEIDGWCDQVDAATRRRLIAPDGSPPI
                     WPQAWTFPTPPVTIPDADRVPFTGVGQPPPPRIDNAIRSLVADGVAALVAAGYDLGTD
                     PDWETAADGRAVRFAGLYRPSPVLLSTARAYSNRRSAYISAVDRVLWLRAVAAAEAAE
                     AAANRDREAKRAKAAQHWAEAGSRD"
     gene            complement(6961..>8522)
                     /locus_tag="AC162A_05445"
     CDS             complement(6961..>8522)
                     /locus_tag="AC162A_05445"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014181566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5010319.1"
                     /translation="IEDVDRPSLTRPLRVPDRVTAHLLGDDSPDPVVEALLTPSVAVE
                     VDEVDVVAHGLRAGLGLVYVRERPGASGQSLGLTALARLGRPALGLDLGRMAAGDDPA
                     LIAVAASREARLRGAGLVVGPVEPLVERGATAVRAFAELPGPVILVGGRDWDPAWSRD
                     PPLVVDAPVPALATRHDLWVTSLNGDQPEGFDPAAATIAFRLTPEQIDRAAQAARHAA
                     TAADRTMTVADVAAGARAQNAAGLERLARRIEPSVAWKDLVLPGVVEAQLRELTARAR
                     HRDRVVGEWRMGTTGARGIGITALFAGDSGTGKTMSAEVVGHDLALDVYVIDLSTVVD
                     KYIGETEKNLDRIFTEADRVNGILLFDEADALFGKRSEVKDARDRYANVEVAYLLQRM
                     ERFDGVAILTTNLRSNVDEAFVRRLDAIIDFPMPEEEHRRLLWERNLAPELPVQDGID
                     LDFLARRFKISGGNIRNVCVTAAYLAAAADRPVSMADLVRATEREYRKLGRLTVEAEF
                     GEYYDLVTATE"
BASE COUNT         1120 a         3123 c         3165 g         1114 t
ORIGIN      
        1 cgctggtcgt cgccgaggag gtggcgcccg aggtgctggc ggagatgcgc gcgctgcggg
       61 gccgcggcgt gcggcggatc gtgctgctcg tgtcgcgcgt ggacgatcgc tcgctcctcg
      121 cggccgcgga ggccggcgtg acgggcgtca tccgccggag cgaggcgacc gcgggcaacc
      181 tggcggccgc gatccggtcg gccgcggcgg gcgagggcac gctgccgccg gacctcctgg
      241 gccgcctgtt gcgccaggtc gggcaactgc agcgccaggt cctgtcaccg cgcgggctga
      301 cgttctccgg cctcaccgag cgcgaggcgg ccgtgctccg gctgctcgcc gacggctacg
      361 acacggccga ggtcggggcg cgcctgttct actccgagcg gaccgtgaag aacatcatcc
      421 acgacgtgac gtcgcggctg gagctgcgca accgcgccca cgccgtcgcg tacgcgatcc
      481 gcgagggcct gatctagcgc cgcttcctca gccgggaacg gcgacgacca ggcgggcggc
      541 gcgctcgccg ccgtcgccgg cggcatcccg aatcgtcgcg aagacccaca cctggagcca
      601 gtcgccgccc gcgagctcga gcccgtgcga gaacgggagg gtcgctcgcc agccggcggc
      661 ggccgatgtc cacgcagggg gcggctcggc ggtgtcctcg tcgcccgcgt cctggagccg
      721 cgcctgccac cggccgagca cgagccctgc ggtgtcgatc cggaccacgc gcagcgtcac
      781 cgacccgagg tcgatctcga ccggtgccgg cccggcctga gtgagggcgg cctcgcggga
      841 cgtcgccgtg agctcgatcg ccgccagcgg atcggcgacg gcgtcctcgt cgagcatgga
      901 acgcacggtc tcgacgagcg ccttccacgg gtccgtcggg cgttcgttgg agcccgtccg
      961 tgtcgtgtgc ccgcccgcct cgagcacctc ggtcgcgccg tcgagcggcg tcgcgacctg
     1021 gacgtcatgc gcgtcgcgaa ccgcgtcgac ggcctcgcgg agcgcctcga cgcgcccgga
     1081 cgccagccgg cccgcgaagg tcccgccccg cgcgccggcg accgtccggc gcgcggtgaa
     1141 cgacccgtcg tccgcgacgt cgagggcctc gtcgtcgctc gggggtcgcc cgccggtccg
     1201 ccggtaccgg accagcgcct cgctcggttc gcccatcagc gccggaccgt ccgccacgtg
     1261 ccgggcctgt tccgccggac gatgtcccgg aagtggcgca cgtgccgctc ggccacgggg
     1321 tggtcatgtg cgctgatcgg cgagttcagg ctggccatgg tgcccatgat gccgcggttg
     1381 ctgtacagga acggctcgac cgccaccgcc tcgttgccgg cggtgtcgaa ctggcccgga
     1441 atccgcgccg cgacgtcgtt gcccatggtc ccggccgggt cgttgacgat cacgaagccg
     1501 gccggaccga agtcctcgcg cagcatgctg cccgcgttcg cggtctcgta ggcgagggcg
     1561 atgcgctggc tgaaggcgcc ctggcggagg ctgtacttcg tgacgaccgc ccgcgccgcc
     1621 gcgccgcggc cggcggccgc ccccgtgacc gccgtgcgca tctccgccgc aatgacctcg
     1681 ggggccacgg ggttgccggc cgcgtcgagg ggggcatcga cccggccgat cggcgcctgc
     1741 tcgccggtca ccgccgcgta ctgctccggt gcccggttgt actcgtcctg gagcccgatg
     1801 aggtgtccga actcgtgcgg cgcgagcccc gggtccgtgt cgcccgtgtg ccagttcgcc
     1861 gagtccgagc gcccgggggt cgtcccggcc ggggtcacga cgaccgccac ggacgcgtcg
     1921 gcgggcgtcc cggtcccgac gacgaactcg aggtccagcg ggtgcccggg gtcgtcgggg
     1981 ttctcgaacc ggaacgtgtt ccacacgctc gtgatgtcgg tacgccactg ggcgacgcgc
     2041 gggtggctgg gcgaccccgt gaagcgcagg ttcacggtca cctggagctt gtcgtcgagg
     2101 aggcgccacg tgaaccgcga cgggcccccg gtgatcgtgc cctcggctcg ctgctcccag
     2161 tcgaacgact cgcggccgac ggtcaccggg cccgccaccg cgtcgagcgc ggaccacgtc
     2221 aacggcccca cgacgccgtc ggcgacgagc ggcggcgttc gggactgctg gaactcgatc
     2281 acggcctgcc gggtgatccg accgaacttg ccgtcaacgt cgagcagcgt cggtacctgg
     2341 tcggacggga tcgtgttgag cttctgctgc agctcctcga ccgcggggcc ggtcgacgtg
     2401 cggcgcaccg tcgggtggat ggtccccccg agcggcgtcc cgcgtggctc gccctcggcc
     2461 gggccttcga cgacgctgcg tccctggcgc gagacgcgcg gggcgagctc gtccaccttc
     2521 gcccacgtcg gcaggccgca gatgccgtcc gggatgagcg gcgggtgcga cgcctggaac
     2581 gcggtcaccg cggccgtggt ctcggggccg tacagcgccg tgatccgcag cggcggcacc
     2641 gccgcgccga gcgcgttcag cgcctgctgc agctccccca cctcgacgct cgagtcgccg
     2701 gggttgaggg cggtcctcgc gggtggggcc ggcgccgcgg cggccccggc gcccgaggct
     2761 ccgggggcca cgggcgtcgc cccgggcgcg tcgtcgcgct ggaggcggag cgaggggcga
     2821 ggcccgagca gcgattgcac ggcggcgttg cccgcgctgc gctggagcgc catgacgacc
     2881 atcgatgagg cggcgtgcac gggcagccgg cgcgggcccg ccccgatcgc gccgaggtcg
     2941 accgcgtcgt cgcgaccggg ggccaggtcg gcggtgccgc cgcgttcccc cggttggcga
     3001 tcgtctccgt gctggacgcg ttgcgcgccc gggcccgcca tcgatcagct ccccggctct
     3061 tcctcttcct cgggcagcgc ctcgcgctgg acggcggcgg gggcggacag cgggtcttcc
     3121 tcccgctgca gcgcgccggc atccgacgcg gacggttgcg aggacgagac cgaggccatc
     3181 acctgctccg cggtcgcgtc ggcggcgcgc tcgaagcggt ccgacgggtc gctgacgcgg
     3241 atgccgccgg ccgcctccga accgtcgacc ggcccacgcg actgctggac gacgtgggcc
     3301 agctcatgcg cgatggtctt ctggcccgat gcactgccgg gatcgaactg gccgctcttg
     3361 aacacgatgt ccgagccgac cgtataggcg ttcgcgccga cggactccgc cgactgcgac
     3421 gcctggtcgt cggtgtggac gcggacgttg tcgaacgact ggccgaacgc cgactccatc
     3481 gtcgactggg tcccggcgtc gagcgccgag ccgccgcccg acccgactac gtcgtggacc
     3541 ggcgaaacct gctcatcctc gcccgccagc atctgcacga ccccggcatt gccggcggtc
     3601 cgctgcaggt gcatcagcgt cgccgcgtcg agcgcgggcg agcgacccgg ggcgaccggg
     3661 gctgactcgg ccgtcgccgg cgtgggcagg tcacgctccg gtgcaatcgg gacgccgcgg
     3721 gcggcttcca actcgaaatc gcgcatggcg gtcctcctgt agctgactac aggatcggcc
     3781 accgcggcag cctccgcaat ggcctcggcc acgaccgccc ggaagtctgc cccaatgggc
     3841 agcccgatcc cggggcacgt cgatacgatc gccggcatgc agacccgcgc cacgcgatcg
     3901 ccggccggcc ggtcgtctgc cgccgccccc gcgaccggcg ggccccggct cacgctggag
     3961 ccggcggcgg ggcccctcgc ggcgagcgca tcgaccatcg aggcgagcgt cctcgatccg
     4021 gccggcgtgg tcgacctcca ggcgaccgcg ggcaaccggg caaccgtcga ggccctccag
     4081 cgcacgcccg ccggcgggac ggccgggccg ggactcacgg tgcagcgcgc agtctccccg
     4141 gaccaggcga cccagatcgc gcggcgcctc gacgacgcga tgcgcggctg gggtaccgac
     4201 gaagaggcga tctacggcgc gctcgccggc cggacggccg ccgacatgga cgagatccgc
     4261 aaggcctaca agagcgtcgc caacaaggac ctcgacgcgg agctgcgcga cgagctcaac
     4321 gactccgagc tcgcgcgcgt cacccagtcg atggcaccga ccgcggacga gtcgaccctc
     4381 accgagggcg aacgggcgac cgccgcgacc gagcgggcga aggtcaccgc cgtccacctc
     4441 aaggaggcga tggcgggctg gggcaccgag gaagacgaga tcttcaacgc gctcacgggt
     4501 cgtgagcccg aggagatcct ggccatccag cgggagtacc tggcgctgac cggcaaccac
     4561 ctcgaccgcg acctgcagga cgagctcagc ggcagcgacc tgcgcaaggc gctcgacctg
     4621 ctgcaggtcc aggcggccgg gaccttcacc aacgagatcg accagcacat gacggagggc
     4681 gagacgacca ccgtccaggg ccggttcgag tacgtgctcc agcagcgcga gctcaccgtc
     4741 cgggtcccgg tcgatttcaa gcccgacgcc ggcgtgacag cgccgatcgc cacctggcag
     4801 tcccagatcg acacggtctg gaacaagttc gccgcagtcg agacaggcgg gctcaagatc
     4861 ccgatccggt tcgggatcga gaaccggtcc ggcgccgaga agtcgatccg ggtgaaggac
     4921 aacaaggtcc cgggcacgca gtcggtggaa gaccgggcga acgccgggat gtggttcccg
     4981 ctcatggacg ccacgaccgc gccgcacgag tttggccacc tcatcggcct ccaggacgag
     5041 taccagcgga cccacggcga ctacaagaag atcaccggcc aggacatcac cggccccgcc
     5101 aacgcctcgg gcaagacggc ggccgagatc gccgtcgatc tccacaatgc gctgtacctg
     5161 cgcgacgcga cccagcgcgc cccgacggcg acgacgctcc tgaccgctgt cggcctcatc
     5221 gtcggcggcg tcccccagca gggcgacttc gcacaggagg tccagaccgc gtacaacgac
     5281 aagtacgacg gctggttctc caagtcgctg gtccaggcga tgcgggacaa gctgcccgag
     5341 ggcagcaagt ggacgatcca gtcggtgttc tcgtacgcca gccggagcat catgggcaac
     5401 ccggaggtcc tgggcggcgt gacgccgcac gaccacgccg tcgagccccg gcacctcgag
     5461 gagttcgtgc gaatcgtccg cggcgcctac ccagacctca cctggacgtc ggggccacga
     5521 tgacgctggt cacgtggcgg cggctgtcgt cgatgccgcc gcccgaacgc gagctcctcg
     5581 acgtcgaagc cgacggctcg ttccggctgt ggcggtcgat cgcgcccgtc gtcggccggt
     5641 tcggcgggtc gctcccggcc ggggtggtct cggagctcga ggggctggtg tcggccgtcg
     5701 ggtcggcggc cgcgccgcgg cccgggtcgc tgccggccga cgccagcgtg gagcagctcg
     5761 aggttggcgg cgcacggtcg gtcttcgagg cgggcgccga ggccgacccg ccgtggggcg
     5821 acctgctggc ggcgtgccgg cggatcgccg atgcgcgcac ggatgcgccc gttgcggcga
     5881 tcgcgcttga ggtcgggctg acggacggac gcgcgcggat ccgcctggtc cagcgcggca
     5941 ccgggacgct cccagtcgag ctcgcgtcgg ggcgtgccaa ggtcgcggtc tggcgcggga
     6001 tggagaaccc ggccaatgcc ctggccgggt tcgatctcgg gcgcgtcgag gccggccccg
     6061 gctgggacgc ggagacgacg gccgagctgc cggtgctcga ggccggcgac aaggttacgg
     6121 ccacggtgac cttcatcgtc gacgacggcg gggtgttcat cccggtcgcg atgtcggcga
     6181 ccgccgtcgc accagcctga ccgtccccta gtcccgggag ccggcctccg cccagtgctg
     6241 ggccgccttc gcgcgcttcg cctcccggtc acgattggcc gccgcctcgg ccgcctcggc
     6301 cgcggcgacc gctcgcaacc acagcacgcg atcgaccgcg ctgatgtagg cgctgcgccg
     6361 gttcgaatat gcgcgcgccg tgctcaacag gaccggcgac ggccgataga ggccggcgaa
     6421 gcggaccgcg cggccgtccg ccgcggtctc ccagtccggg tcggtgccga ggtcgtaccc
     6481 ggccgcgacg agggcggcga cgccgtccgc gaccagtgac cggatcgcgt tgtcgatccg
     6541 cggtgggggc ggctggccga ccccggtgaa cggaacacgg tcggcatcgg ggatggtcac
     6601 cggcggggtc ggaaacgtcc acgcctgggg ccagatcggc gggctaccgt ccggggcgat
     6661 caggcgccgg cgcgtcgccg cgtccacctg gtcgcaccag ccgtcgatct cggcccgcag
     6721 ctgcctgagg cccttggcgc ccagctcgag gctgtggtcg gcctgcgcct ccacgatttg
     6781 cgcggccgcg gtcaggaccg cgtcggcgac gacccgggcg acggccttga acaggtcgtt
     6841 cgcggcgtca ctgagcgcct ttgcgcgcac gtcgagggcg gcgagcgcct catcgagcgg
     6901 gtcgggcgtt gcggatcggt cagcgggcgg cggcagcatc ggacgtctcc atgagcacct
     6961 tcactcggtg gccgtgacca ggtcgtagta ctcgccgaac tccgcctcga cggtgagccg
     7021 gccgagcttg cggtactcgc gctcggttgc gcggacgagg tcggccatgc tgaccgggcg
     7081 gtcggcggcg gcggccaggt aggcggcggt cacgcacacg ttgcggatgt tgccgccgga
     7141 gatcttgaat cggcgggcga ggaagtcgag gtcgatgccg tcctgcacgg ggagctcggg
     7201 cgccaggttg cgctcccaca gcagccgccg gtgctcctct tcgggcatcg ggaagtcgat
     7261 gatcgcgtcg agccgccgca cgaacgcctc gtcgacgttg gaacgcaggt tggtcgtcag
     7321 gatcgcgacg ccgtcgaagc gctccatccg ctggagcagg taggcgacct cgacgttcgc
     7381 gtaccggtcg cgggcgtcct tgacctccga acgcttgccg aacagcgcgt ccgcctcgtc
     7441 gaacagcagg atcccgttga ctcggtcggc ctcggtgaag atccggtcga ggttcttctc
     7501 ggtctcgccg atgtacttgt cgacgaccgt cgagaggtcg atcacgtaga cgtcgagcgc
     7561 caggtcgtgg ccaacgacct cggccgacat cgtcttgccg gtcccggagt cgccggcgaa
     7621 cagggccgtg atcccgatcc cgcgcgcgcc ggtcgtgccc atgcgccact ccccgaccac
     7681 gcggtcgcgg tgccgggccc gggcggtgag ctcgcgcagc tgggcctcga cgacccccgg
     7741 cagcacgagg tccttccagg caacggaggg ctcgatccgt cgcgcgaggc gctcgaggcc
     7801 ggcggcgttc tgggcacgcg cgccggccgc gacgtcggcc acggtcatcg tgcggtcggc
     7861 ggccgtcgcg gcgtggcggg cggcctgggc ggcccggtcg atctgctcgg gcgtgagccg
     7921 gaaggcgatc gtcgccgcgg cggggtcgaa gccctcgggc tggtcgccgt tgagcgaggt
     7981 gacccacagg tcgtggcggg tcgccagcgc cggtaccggc gcgtccacga cgagcggcgg
     8041 atcgcgcgac caggcggggt cccagtcacg ccccccgacc aggatcaccg gacccggcag
     8101 ctccgcgaac gcgcggacgg ccgtcgcacc gcgctccacg agcggctcga ccggcccgac
     8161 gaccagcccg gcgccgcgca gccgtgcctc gcgggacgcc gcaacagcta tgagtgccgg
     8221 atcgtcgccc gccgccatcc gcccgaggtc gagcccgagc gccggacggc cgaggcgcgc
     8281 gagtgccgtg aggccaagcg actggccgga ggcgccgggg cgctcccgga cgtacacgag
     8341 cccgaggccg gccctgagtc cgtgcgcgac gacgtcaacc tcgtcgacct cgacggccac
     8401 cgacggggtg agcagcgctt ccacgacggg gtccgggctg tcgtcgccca ggaggtgggc
     8461 cgtgacccga tccgggaccc gcagcgggcg ggtgagcgac gggcggtcga cgtcctcgat
     8521 ga
//
LOCUS       DBNHXO010000264        18405 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5430242, whole genome shotgun sequence.
ACCESSION   DBNHXO010000264 DBNHXO010000000
VERSION     DBNHXO010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 18405)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18405)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18405
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5430242"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="AC162A_05450"
     CDS             complement(<1..614)
                     /locus_tag="AC162A_05450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5010320.1"
                     /translation="MVVDLLCNSPYYCGALVAALRTEGVLAELASPAFYLEPDYLAAY
                     PVAPWITNLTVRVARPRPLRLASRTVEVALNGMRLLWRINRRRYDVVHVQWMPLDHRQ
                     AVGMRLLRTVCDRAGARLVFTAHNVLPHDDGGADLAIVRRNLNFAHLVVAQTDHVARE
                     LRTTIRTRTPIVEIPHGPLFADRPLPAPADAAARLDWPPDPTIL"
     gene            complement(623..817)
                     /locus_tag="AC162A_05455"
     CDS             complement(623..817)
                     /locus_tag="AC162A_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010321.1"
                     /translation="MVNPARANRLPAAQLIAESRLAGGVSGSCEDVAAELGPMAEIAC
                     QCRADLLDAFGLAQPGVERP"
     gene            959..2308
                     /locus_tag="AC162A_05460"
     CDS             959..2308
                     /locus_tag="AC162A_05460"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR5010322.1"
                     /translation="MSERSAQFPDASLPVLARPGGFALIPLFAFVALIPIEEALSVSG
                     VGTVSRGVAVIFALLYVVPRIGRLTLRAMPLAGWAFMEWAVLSSLWSINANVALAELS
                     TLIQLFAIAVLVADVIVHDPGLVRPLLWTYSISASFLAALGFLAFLEGNRVANDRVVA
                     LAGQDPAHFAALLLPAFIFCTFELLRGRRPYLSLSVVLISLAGILLSGTRGAWVAMVV
                     ALALFVFPALDLRRKVGAVILLVVVFGAVLQIPGVGNLVVDRVATAAASGGAGRTDIW
                     KVGVVIFETSPVIGVGYANFPVAYTPKMVMEAGVGLAYQGAYRDPHNLVVSIGAELGL
                     VGLALFGLFLVPLVLRRGWGPDAQVIRAILAAFMVDALFLDLFGNRKQVWLIIGIAAG
                     LAFLARRAESADEEAGAADGRAVAVAVRPSLDLHRPRQSGGRPASSAPPGSMIRGDS"
     gene            2419..4089
                     /locus_tag="AC162A_05465"
     CDS             2419..4089
                     /locus_tag="AC162A_05465"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase domain-containing
                     protein"
                     /protein_id="HIR5010323.1"
                     /translation="MDVQTAVRVLRRRWWLPVLFVVAAAVPAYLVSRSLPKVYEADTT
                     LLVGLGLPGTSPDYQQLLASQQLSQTYSSIVTTGPLLEAVKEDLDLDMTIDALRKSVT
                     ATAPVESTLITITASDGDPARAAQIADALAQELISRSPSISTSDPAVRRIVTDLLDGD
                     LQSMQEQISTAQSEITRLAGLSNPTPEDAGQLAALRGQLPSLQQAYATILGYASSGDA
                     NILTVVDPAQVPTEPTSPRVALNTLILALLALVNAIALVFLLEYLDDTLKTPDDVEAL
                     IGLPTIGTITLRRLGPSNSPRDGLATLLYPRGEAAEAYRTLRTNVDIANVDDPIRVVL
                     VTSAVPKEGKTTTAANLAVAIAQTGSNTILLDADLRSPGVHRMFDLPNHKGMTSLLGP
                     DDANFEWVAQATEQPYLRVITTGPLPVNPVEVLASRRMARFLEALRARVDFIVIDSPP
                     LQGLSDAAVLSTLVDGTVLVVSAGRTRRNAVRDAAKALAARGGRTLGVVLDRQSKRDE
                     QDSDADRYGVKGAPAAAQADEQATLASARGALRPDPAAIRLPPDDVRQ"
     gene            4111..5073
                     /locus_tag="AC162A_05470"
     CDS             4111..5073
                     /locus_tag="AC162A_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5010324.1"
                     /translation="MATDGDRGVRALVTGGAGFIGHHLVRGLLAHGHDVAVLDDFSSG
                     SPERLSALRDRISLVEGSVLDADAVDRAIAGRDIVFHLAAIASVARSLREPRLTHAVN
                     VEGTIAVVLAAARHHLRRAVLAGSSAVYGDTVTLPCREEQRPAPQSPYGVDKLAAEHY
                     LHVLGGSRGVETVALRYFNVFGPGQDPASEYAAVIPRFISAALRGQRPTINGSGTISR
                     DFVHVDDVVAANLLAATVPGVGGLTCNIACGAPTTLDQLVTAVGAATGVALEPIYGPP
                     RVGDVVASWADVSRASEILGYAAAVPFEAGVARTVAWFRTPAPA"
     gene            complement(5177..6238)
                     /locus_tag="AC162A_05475"
     CDS             complement(5177..6238)
                     /locus_tag="AC162A_05475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5010325.1"
                     /translation="MERVVETLAVGLHRRGHDVSVATLLLGRSADHPFVQALQETGVK
                     LHLLPMAPRDYLRERRELRALFASLRPDVVHTHGYRVDLLDRGIAAGLGIPTVTTVHG
                     ASMTGGAKGKLFEWLQRMNYRRFDAVVVVSRQLQERTLADGVSASRLHLVPNAWQPRQ
                     EPLERSAARRALGLDDATDVVGWVGRFIPVKGADVFLEALARIPEPRPLGVLVGYGPE
                     SERLRQMASELGLGSSIRFCSDVRDAAPFFRAFDTYVLSSRSEGLPIVMLEAMSAGVP
                     IVATTVGGVPEALADGAGWLVPPSEPAALASAIAESLRDRAAALECARRASRRFENDY
                     SVETFLDRYEQAYRQVLRG"
     gene            complement(6294..7364)
                     /locus_tag="AC162A_05480"
     CDS             complement(6294..7364)
                     /locus_tag="AC162A_05480"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5010326.1"
                     /translation="MSDQRTVLVVGAGGFIGGRIVEVLHQLGADHVRAGLRRWAGGAR
                     VGRLPVELVQVDIRDDGDLRRALDGVTHVVNCAVGDPSTTVDGTRKLLTAAHASGAQR
                     VVHISTVDVYGQPEGEVDESRPLTITGRPYGDSKIEAERACQELAAKGLPVTILRPTL
                     VHGPFSATWTVPYAQRMLKRPWLIPEAVSQGTCNLLYVDDLASAVLAALSADTTPGDA
                     FNVNGPERPTWHAYFTQLNDALGLPPLVDATPSGARVRSAAVQPLRKSAKFLINHFEP
                     QIMRVGQRSLLARNTMLRAERLVRTTPSPAEFALYQRQASFSTSKAQDILGYRPACPM
                     ATALPLTAAWLRHHGYVTGPGD"
     gene            complement(7357..8367)
                     /locus_tag="AC162A_05485"
     CDS             complement(7357..8367)
                     /locus_tag="AC162A_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5010327.1"
                     /translation="MPDLPIALIGCGAIAEMFYLPALEKRPDLLSSLILVDRNAEKAE
                     AVRKRLGAAASASDHREVLSRVKGAIIATPHHLHTPLTLEFLEHGVHVLCEKPLSRTA
                     AEVDQVVAAAAANRVHVLVNQTRRLYTSFQEVKRRIANGSIGELREIDYVLGEPFGWP
                     AETNTYFGVAAGGRGVLFDNGAHIVDLVCWFLGGEPEIVSYEDDSRGGTEAVAEIKLR
                     LGDANARIRLSWLSKLRNTYRITGTRGSMDGRVYESSSFTFRDPSGKSRRIKTDGARE
                     GSYFAGKLLANFVGVVEGREQPIVAAGDVRPAISVIDACYQRRSTMIEPWYDVAGTPA
                     SV"
     gene            complement(8445..9050)
                     /locus_tag="AC162A_05490"
     CDS             complement(8445..9050)
                     /locus_tag="AC162A_05490"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR5010328.1"
                     /translation="MARRLTDIVLALIGLAVLAPLLLAIAAAVRLRDGAPVIYKARRV
                     GQHGREFRLFKFRTMVPAGGSSVTVWADPRVTPLGRRLRRLKLDELPQLLNVLRGEMS
                     IVGPRPEDPSYVAIYTDEQRRVLSVKPGITGVAALAYADEESLLQGEDWEATYRLTIM
                     PAKLQLELDYLARRTARTDAELILQTVAVVVGHLGPRRQSH"
     gene            9185..10150
                     /locus_tag="AC162A_05495"
     CDS             9185..10150
                     /locus_tag="AC162A_05495"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5010329.1"
                     /translation="MSTVLVAGGAGYIGSMVSRELLARGHRVVAADALLFGGEALLDL
                     LSHPDFAFRKVDVRDPQDLEALFSTWQFDAVVHLAAIVGDPACKAQPELATQTLWDGS
                     RSLLELSDQHGVGHFVFASTCSNYGRMESNAVLDEDAPLRPVSLYAELKVRFEELLMA
                     RSVALDFTILRFATVYGLSLRPRFDLTINEFARDALLKGELEVFGPDFWRPYCHVRDI
                     ANAVTMILEQDRGRVAGRVFNVGADDENYQKRAIAEAVAEQIPVRLRYVERAEDPRDY
                     RVSFERIAALGFTPRMRLRDGIREVAEAIATGLIADPYAARYRNS"
     gene            10164..11327
                     /locus_tag="AC162A_05500"
     CDS             10164..11327
                     /locus_tag="AC162A_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HIR5010330.1"
                     /translation="MIPLSEPSLGGRELEYITRCLDTRWISANGEFVWEMEKFLASYL
                     GVKHVVACNSGTSAIHISLMLSGVQPDDEVIVPTVTFIAPVNAVRYVHAWPVFVDCDE
                     FCNMDVEGVRSFLADECVVRDGVLTNRTTGRRVSAIVPVHVFGTSVDMDPILALAEEY
                     GLAVVEDASESLGSRYKGRMCGTLAPISCLSFNGNKIVTTGGGGAIMTDDDEIARQAR
                     YLTTQAKEDGLEYIHNSVGYNYRMNNVLAAIGLAQLEDLDERLAIKRANFARYQEALG
                     PSRLLDQPEWSESNRWFYALLCDSADMKRELLEACTAAGVQVRPLWYLNHLQQPYRET
                     QAHRITRAHHFYDTVLNLPCSISLTPQQIAEVVALVEAAKRGAPEDAIARTRA"
     gene            11336..12355
                     /locus_tag="AC162A_05505"
     CDS             11336..12355
                     /locus_tag="AC162A_05505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5010331.1"
                     /translation="MGGRIPTITVGIATLDRYPYVADLLDDLAAQTHLPDEVVIVDQT
                     APHRRQPFETTRWSHAFPMRVVVQETRGTTKARNRLLRECRSEVILQPDDDSRIDPDY
                     VEHHVQHYADDRVDVVSGPVYEWDADTREWYVKWSNHLMIGGWNGPAFASGHSFAGGN
                     SSIRVASALAIGGWDENILTHGEDEDLSDRLLAADAVIVYDPKARLLHLRAPRGGERA
                     AEWGSGPDGNWRVLAGYLYYFLANRPPYDAWEMFREYTIRPFWHIKHRRTSVGLRLLP
                     RVAAAIPVSTWRWVRGRRLIDPASPDREVRRIPKKEVAQWRRRLAAKRPRTVLPDRDP
                     PQLPL"
     gene            complement(12441..14255)
                     /locus_tag="AC162A_05510"
     CDS             complement(12441..14255)
                     /locus_tag="AC162A_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5010332.1"
                     /translation="MLSRRERRNYWVAVGLQMATSLLDLAGVLLFGMVGVIAASAAQG
                     VPLPDAVEEVLSWFGLADVPATTASLVLAGIAALLLVLKTVASLWILRWTTRFLTTAS
                     TRISAQMCADFYALPIVHVNRFESQWSAFALVHGVSGAIVETLTNAMVIWVELSLLVV
                     LGGALLLLNPLIALFAVGYFGLIAVLMNQVLRGWAYTTGKVMAESSWESNVTVQDGIA
                     TYREITVADRRQHYIDRFRSLRQAGSEAYADQQFISQVPRYGMEIALVVGAGLLVLAL
                     LQHDTVESALGSLALFLTAATRVMPSLLRLNGSRITLRSLVPRADYAFTMAAHIREHA
                     TPAILETTPPAAPATEVGAGRPGKPDLVLEVAVRDLKVDYPGSDAPAIEGITFTLPPG
                     GALAVVGPSGGGKSTLADAILGVVQPSAGLVRIGGLSPGQVVRRYPGLVAYVPQSVAL
                     VSGSVRDNVALGRTRDEVNDDQVWAALKRAHLSDYLADARDGLDTEVGERGVQLSGGQ
                     RQRLGIARALYTDPRLLVMDEATSALDAEIENLVTQTIEELGSEVTTITIAHRLATIR
                     RADTVIYLDRGRLVAQGTFEEVRAVVPQFDRQANLLGL"
     gene            14794..15462
                     /locus_tag="AC162A_05515"
     CDS             14794..15462
                     /locus_tag="AC162A_05515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HIR5010333.1"
                     /translation="MPDPEVRERPIRRSRTVVVLGGGGHAKVLISVVRKLPWTILGYV
                     DPRDVGPVLGVPHAGGDDVLPALLARNPGCAAAMGVGKVDATARRAGIQAAAEALGYR
                     FPTFVSPDALVNDEVELGAGTAVFDGAVLNPGVITGPTCVVNTNATVEHDCQFGTNVH
                     IGPGATVSGGVTIGDHTFVGAGAVVIHGVRIAEACMIGAGAVVTGDLTEPGTYVGAPA
                     RRIR"
     gene            15459..16154
                     /locus_tag="AC162A_05520"
     CDS             15459..16154
                     /locus_tag="AC162A_05520"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HIR5010334.1"
                     /translation="MSAGSALAVIPARGGSRRVPRKNIREMHGRPLLAYSVEAALRSS
                     AFARVVVSTDDAEIAAVARAAGADVPFLRSPALADDVTPVSRATIDVVDRLEADGESY
                     LEVCQLMPNCPLRTADDVVASHRQFTDQPAEAQISVARYGWQSPWWAMRRAADGSLDP
                     LFPDAMRQRSQDLPELLCPTGAVWWARTDALRREGTFHLAGRTGWEIDWTHAIDIDTE
                     EDWRLAEALMGRS"
     gene            16160..17068
                     /locus_tag="AC162A_05525"
     CDS             16160..17068
                     /locus_tag="AC162A_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR5010335.1"
                     /translation="MALYTAVYPGAARFLASWYASVQAQADRDFDLWISLDGLTEAAV
                     VARLGDRPAATFVVAEAGFTPAQVRQQALARIADRYDAVVLVDSDDLLHPDRVAAARA
                     WLEGADVAACGLRLVDEAGQPLGVTLATPGEGEVDSILPRANAFGLSNTAYRSDVLRS
                     CLPISAAAALVDWYLVTMAWLQGARLAFDPTPRMDYRQHGANMARIRSPFTAERVQAD
                     TALVREHFRLVLDGVPAGALPARVTQVREAAAEVATFDQRVVEDSAALAIYVRDLNAA
                     PPPLLWWASVAHPPLRFQWSPQKEPT"
     gene            17065..18117
                     /locus_tag="AC162A_05530"
     CDS             17065..18117
                     /locus_tag="AC162A_05530"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0070085 - obsolete glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HIR5010336.1"
                     /translation="MRPVNLGPVAVGGDNPPYIVAEVGSNHNGDMDLCMRLIDAAAEA
                     GADAVKFQSWSETSLIAREEYERNPSYSDTKKHFGSLREMVRAYQLTPEQHVVARDHA
                     RERGIAFASSAFAPAEVDLLAGLDVPFIKIASMDINNLPLLAYVARSGKPVVISVGMA
                     SLGEIERAMDTVRAEGNEDIVLLHCVSIYPAPPSTVHLRNLETLRNAFDVPVGFSDHT
                     LGTAIPLAAIALGACLIEKHFTLDREMEGWDHDISSDPPVLRALVEEGRIIHEALGST
                     RRMLSEAEIVKRRQFRRSLVTRTALPAGHILAEADLDAKRPGTGISPDELRYTVGREL
                     AADLAEDQVLRWEHLR"
     gene            18146..>18405
                     /locus_tag="AC162A_05535"
     CDS             18146..>18405
                     /locus_tag="AC162A_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010337.1"
                     /translation="MARPVYVVHCVDTEGPLHESVEATFERLEAIFGIDLEPSVALLR
                     QLQAGEVDLGGIEQAVRKVVDPHLLAYNDTWDKVNEMLASCMS"
BASE COUNT         2866 a         6488 c         6242 g         2809 t
ORIGIN      
        1 aacaggatcg tcggatccgg cggccagtcc aggcgcgcgg cggcgtccgc cggggccgga
       61 agcggccggt ccgcgaacag cggtccgtgt gggatctcga cgatcggcgt ccgcgtccgg
      121 atcgtcgtcc ggagctcgcg ggccacatgg tcggtttggg caacaaccag atgggcgaag
      181 ttcaggttcc ggcgcacgat cgccaggtcc gcgccgccgt cgtcgtgggg aagcacgttg
      241 tgagcggtga ataccaggcg tgccccggca cggtcgcaga cggttcgcag gaggcgcatg
      301 cccaccgcct ggcgatggtc aagcggcatc cattgcacgt gcacgacgtc atagcggcgg
      361 cggttgatcc gccacagcag gcgcatcccg ttgagggcga cctcgacggt gcggctggcc
      421 agacgcaggg gccgcggccg ggcgacacgg acggtcaagt tggtgatcca cggtgcgacc
      481 gggtaggccg cgaggtagtc cggctccagg tagaaggcgg gacttgccag ctcggccaga
      541 acgccctccg tgcgcaacgc ggcgacaagc gccccgcagt agtagggcga gttgcagagg
      601 aggtccacga ccacggccct cgtcatggcc gttccacgcc cggttgggcc aggccgaacg
      661 cgtcgagcag gtccgcccgg cactggcacg cgatctcggc catgggtccg agctcggcgg
      721 cgacgtcctc gcaggagccg gagactccac cggcaagccg gctctcggcg atcagctgcg
      781 cggccgggag acggttcgcc cgcgccggat tcaccatcgc cctgctgcct cctcggcccg
      841 acccctgccg ccacattcga gcgagagcgc gggagcgcct atcatgctgc atcggccgta
      901 acgatggccg gctcgtggaa gggccggccc gggcgggaca agggaagtca ccagccacgt
      961 gagcgagcga tcagcccagt tcccggacgc gtcgttgccg gtcctcgcgc ggcccggcgg
     1021 gttcgccctg atccccctgt tcgctttcgt ggccctgatt ccgatcgaag aggccctgtc
     1081 ggtgagcggc gtcggcaccg tcagtcgcgg cgtggcggtg atcttcgccc tgctctacgt
     1141 ggttccccgc atcggccggc tgaccctccg ggcgatgccg ctggcgggct gggccttcat
     1201 ggagtgggcc gtcctgagca gcctctggtc catcaatgcc aacgtcgccc tggccgagct
     1261 gtcgacgctc atccaactgt tcgcgatcgc ggtcctggtc gcggacgtca tcgtccacga
     1321 ccccggcctg gtgcggcccc tgctgtggac ctacagcatc tccgcttcgt tcctcgccgc
     1381 gctcggcttc ctcgccttcc tggagggaaa tcgagtggcg aacgaccgcg tcgtcgccct
     1441 ggcaggccag gatcctgccc acttcgccgc gctcctcctg cccgccttca tcttctgcac
     1501 cttcgagctg ctgcgcggcc ggcggccata cctcagcctt tcggtggtcc tgatcagcct
     1561 tgccggcatc ctcctgtcgg gcacccgcgg agcctgggtc gccatggtcg tcgccctcgc
     1621 cctcttcgtc ttcccggccc tggacctgcg ccgcaaggtc ggcgccgtca tcctgctggt
     1681 ggtggtgttc ggggccgtgc tgcagatccc gggcgtgggc aacctggtcg tggaccgggt
     1741 ggcgaccgcc gcggccagcg ggggcgcggg gcggaccgac atctggaagg tgggcgtggt
     1801 catcttcgag acctcgccgg tcatcggggt gggctacgcc aacttcccgg tcgcctatac
     1861 cccgaagatg gtgatggaag ccggcgtcgg gttggcctac cagggcgcct accgcgatcc
     1921 ccacaatctc gttgtctcga tcggggcgga attgggcctc gtgggcctgg cgctctttgg
     1981 cctgttcttg gtgccgctgg tcttgcgacg gggctggggt ccggacgcgc aggtcatccg
     2041 ggcgattctc gcggccttca tggtggacgc gctcttcctg gacctgttcg gcaaccgaaa
     2101 gcaggtgtgg ctgatcatcg gcatcgccgc ggggcttgcc ttcctggccc ggcgggcgga
     2161 atccgcggac gaggaggccg gcgcggcgga tggacgtgcg gtcgccgtgg ccgtccggcc
     2221 ttcgctggac cttcatcgcc cacgtcagtc gggcggccgc ccggcatcga gtgccccgcc
     2281 ggggtcgatg atccgcggag attcctgagg ccctccccgc tccatccggc ccgccgacgc
     2341 gaagtcggtc acaatacggg cgcgcacgac gcgacccgca cgaaccagag gctgtccctg
     2401 gcaatggcag aacgacacat ggacgtacag acggcggtgc gcgtactccg ccgtcgctgg
     2461 tggctccccg tgctcttcgt cgtcgccgcc gccgtgcccg cctacctggt cagcaggtcc
     2521 ctgcccaagg tctacgaggc ggacacgacc ctgctggtag gccttgggct gcccggcacg
     2581 agcccggatt accagcagct cctggccagc cagcagctca gccagacgta ttccagcatc
     2641 gtcacgacgg gccccctcct cgaggcggtc aaggaagacc tggatcttga catgaccatc
     2701 gacgcgctcc gcaagtcggt cacggcgacg gcgcccgtcg agtccaccct gatcacgatt
     2761 acggcctccg acggcgaccc ggcccgcgcc gcgcagatcg ccgacgcgct ggcccaggag
     2821 ctgatctcgc gcagcccgtc gatctccacc agcgacccgg cggtgcggcg gatcgtcacc
     2881 gacctgctgg atggcgacct gcagagcatg caggagcaga tctccacagc acaatcggag
     2941 atcacccggc tggcggggct cagcaacccc acccccgagg acgccggcca acttgcggca
     3001 ctgcggggcc agctcccctc gctccagcag gcctatgcca ccatcctggg ctatgccagc
     3061 agcggcgatg ccaacatcct gaccgtcgtc gatccagccc aggtcccgac cgagccgacc
     3121 agcccccggg tcgccctcaa caccctgatc ctcgccctgc tcgcgctggt caacgcaatc
     3181 gccctggtgt tcctcctgga atacctggac gacacgctga agaccccgga cgacgtcgag
     3241 gccctgatcg gcctgcccac gatcggcacg atcacgctca ggcggctggg ccccagcaac
     3301 agcccgaggg acggtttggc aaccctgctc tacccgcgcg gcgaggccgc ggaagcgtac
     3361 cgaacccttc gcaccaacgt ggacatcgcc aatgtcgatg acccgatccg ggtggtcctg
     3421 gtcaccagcg ccgtgccaaa ggagggcaag accaccacgg ccgccaacct ggccgtggcg
     3481 attgcccaga ccggtagcaa cacgatcctc ctggacgccg acctgcgcag tccgggagtg
     3541 catcggatgt tcgacctccc caaccacaag ggcatgacct cccttctcgg acccgacgac
     3601 gccaacttcg agtgggtcgc ccaggcaacg gagcagccgt acctgcgcgt catcacgacc
     3661 ggcccactgc cggtcaaccc agtggaggtg ctcgcgtcgc ggcgcatggc acgcttcctc
     3721 gaggcgctca gggcgcgggt ggacttcatc gtgatcgaca gcccgccgct ccagggcttg
     3781 tccgatgcgg cagtcctgtc caccctggtc gacggcacgg tcctggtcgt gtcggccggc
     3841 cgcactcggc gcaatgccgt ccgtgacgcc gcgaaggctc tggcggcccg aggcgggcgg
     3901 acgctggggg tggtcctgga ccggcagagc aagcgcgacg agcaggactc cgacgcggac
     3961 cggtatggcg tcaagggcgc gccggcagcg gcgcaagctg acgagcaggc gacactggcg
     4021 tctgcccggg gcgccctgcg accggaccct gccgccatcc gccttccacc ggatgacgtc
     4081 cgccagtgag ccgccgcggc gaccggcggc gtggccaccg acggtgaccg cggcgtgagg
     4141 gcgctggtca ccggtggcgc cggcttcatc ggtcaccatt tggtccgggg cctcctggcg
     4201 cacggccacg acgtggcggt cctggatgac ttctcgtcgg gctcgccgga gcgactttcg
     4261 gcactgcgcg accggatcag cctggtcgag ggaagcgtcc tggacgccga cgcggtggac
     4321 cgcgcgatcg ccggccggga tatcgtcttc cacctggccg cgatcgcctc ggtcgcccgc
     4381 tccctgcgcg agcctcgtct gacgcacgcc gtcaacgtgg aaggcacgat cgccgtcgtg
     4441 ctggcagccg cgcgccatca cctgcggcgc gccgtcctgg cgggctcgtc ggcggtttac
     4501 ggcgacaccg tcacgctccc ctgcagggag gagcagcggc ccgctcccca atcgccgtac
     4561 ggggtcgaca agcttgcggc cgagcactac ctccacgtcc tgggcggcag ccgtggcgtg
     4621 gagaccgtcg cccttcgcta tttcaacgtg ttcggaccgg gccaggaccc cgcgtccgag
     4681 tacgcggcag tgatcccacg attcatcagc gctgctctgc gcggccagcg cccgaccatc
     4741 aacggcagcg gcaccatctc gcgcgacttc gtccacgtcg acgacgtggt ggcggccaac
     4801 ctgttggcgg ccaccgtgcc gggcgtcggc ggtctcacct gcaacatcgc ctgcggcgca
     4861 ccgaccacgc tggaccagct ggtgacggcc gtcggcgccg cgaccggcgt cgccctggag
     4921 ccgatctacg gtccgccccg tgtcggcgac gtcgtcgcgt cctgggcgga tgtctcgcgg
     4981 gcgagcgaga tcctgggata cgcggcagct gtcccgttcg aggccggggt cgcgcggacc
     5041 gtggcctggt tccgaacccc ggcaccggcg tgatcgaatc gaccgcgcct tgacgagtcg
     5101 gacggcgcgt agccagcgca actcgggtgt cgtcgggcaa aggttgctcc gccgctttcc
     5161 ccgcacccag aggcatctat ccgcgcagca cctgccggta tgcctgctcg taacggtcga
     5221 ggaacgtttc gactgaatag tcgttctcga accttcggct cgcgcgtctc gcacattcga
     5281 gcgcggcggc ccgatcgcgg agcgattccg cgatggcact ggcgagcgcc gcgggttccg
     5341 agggcggaac aagccacccg gcgccgtcgg cgagtgcctc cgggaccccg cccaccgtcg
     5401 tcgcgacgat gggcacgccg gccgacatcg cttcgagcat cacgatcggg agcccctccg
     5461 atcgcgagct gagcacgtag gtgtcgaacg cgcgaaagaa cggggcggcg tcgcgcacgt
     5521 ccgagcagaa ccggatggac gagccgagcc ccagttcgct cgccatctgg cggagtcgtt
     5581 ccgattccgg gccatacccg acgaggacac cgagcgggcg gggttcgggg atgcgggcca
     5641 acgcctcgag gaagacgtcg gcgcccttca ccggaatgaa tcggcccacc caccccacga
     5701 cgtcggtcgc gtcgtccagt ccgagggcgc gccgagcggc gcttcgctcc aacggctctt
     5761 gtcgtggctg ccatgcgttc ggcacgaggt ggagacgcga ggcactcacg ccgtctgcga
     5821 gcgtgcgttc ctgcagctgc cgggagacca cgactaccgc gtcgaagcgt cggtaattca
     5881 tgcgctggag ccactcgaag agcttgccct tcgcaccgcc cgtcatgctg gcgccgtgca
     5941 cggtggtgac cgtgggaatc ccaagacctg ccgcgatgcc acggtcgaga aggtcgaccc
     6001 ggtacccgtg cgtgtggacg acgtccggcc gcaatgacgc gaacaacgcg cgcagctccc
     6061 gtcgctcccg aaggtagtcg cgcggggcca tgggcaggag gtggagcttc acgcctgttt
     6121 cctggagcgc ctgcacgaac ggatgatccg cagacctccc caggaggagg gtggccacgc
     6181 tgacatcatg accgcgccga tgcagcccga ccgcgagcgt ttccacgaca cgctccatac
     6241 cgccggcctc agccggtgcg gcgacttgga cgatcttcaa tgctcgcgag ctctcagtcc
     6301 ccggggcccg tgacgtaccc gtggtgcctc agccacgccg ccgtcaacgg cagtgccgtc
     6361 gccatcggac aggcaggtcg gtagccgaga atgtcctgcg ccttcgacgt cgagaacgac
     6421 gcctgtcgct ggtagagggc gaactccgcg ggggaaggcg tcgtccggac gaggcgttcg
     6481 gcccggagca tcgtgtttcg ggccaggagc gatcgctgcc cgacacgcat gatctgcggc
     6541 tcgaagtgat tgatcaggaa cttggcgctc ttccgcagcg gctgcaccgc ggccgagcga
     6601 acgcgagcac ccgacggggt ggcgtcgacg agcggcggca gcccgagcgc gtcgttcagc
     6661 tgcgtgaagt acgcgtgcca ggtgggccgc tccgggccat tcacgttgaa ggcgtcgccg
     6721 ggcgtcgtgt cggcggacag ggcggccagc acggcggacg cgagatcatc cacgtagagc
     6781 aggttgcagg tcccctgcga gacggcttcc gggatgagcc agggtcgctt cagcatgcgc
     6841 tgcgcatacg gcaccgtcca cgtcgcggag aacggaccat gcaccagcgt cgggcgcagg
     6901 atcgtgaccg gcaagccctt ggcggccagc tcctggcacg ctcgctcggc ttcgatcttc
     6961 gagtcgccgt acggtcggcc ggtgatcgtc agcggtcgag actcatccac ctctccctcg
     7021 ggctgaccat agacatcgac cgtcgagata tggacgactc tctgcgcccc cgacgcatgc
     7081 gccgcggtga gcagcttcct cgtcccatcg accgtggtcg acgggtcacc caccgcgcag
     7141 ttgacgacat gcgtcacacc gtcgagcgct cgccggaggt cgccgtcgtc ccggatgtcg
     7201 acctggacga gctcgaccgg cagccgtccc acgcgagccc cgcccgccca ccgccgcaag
     7261 ccggcgcgga catgatcggc tccgagctgg tgcaggacct cgacgatgcg gcctccgatg
     7321 aagccaccgg cacccacgac gagcaccgtg cgctggtcag acactggccg gggtccccgc
     7381 gacgtcgtac cagggctcga tcatcgtcga tcgccgttgg tagcacgcgt cgatgaccga
     7441 gatcgccggg cggacgtcac cagccgcaac gatcggctgc tcgcgtcctt cgaccacgcc
     7501 cacgaagttg gccaggagct tgcccgcgaa gtagctccct tcgcgagctc cgtcggtctt
     7561 gatcctgcgg gacttcccgc tcgggtcgcg gaaggtgaag gacgaggact cgtacaccct
     7621 tccgtccatc gagccacgcg tccccgtgat gcgatacgtg ttccgaagct tgctcagcca
     7681 ggagagccgg atccgggcgt tggcgtcgcc gagccgaagc ttgatctcgg cgacggcttc
     7741 ggtgccgccc cgggaatcgt cctcgtagct gacgatctcg ggctctcccc ccaggaacca
     7801 gcagacgagg tcgacgatgt gggctccatt gtcgaacagg acaccgcggc cgccggcggc
     7861 gacgccgaaa taggtgttgg tctcagccgg ccatccgaag ggctcgccca gcacgtagtc
     7921 gatctcgcgc agctcaccga tggacccgtt ggcgatgcgg cgcttcactt cctggaagga
     7981 ggtgtacaac cggcgcgtct ggttcacgag cacgtggacg cgattcgcgg ccgcagccgc
     8041 gacgacctgg tccacctccg cggctgtgcg ggagaggggc ttctcgcaca gcacgtggac
     8101 gccgtgctcg aggaactcca gcgtcagcgg cgtgtggagg tgatgcgggg tcgcgatgat
     8161 cgcgcccttc acgcgcgaca ggacctcccg gtggtcgctg gcggaggcgg cggcgccaag
     8221 ccgcttgcgt accgcttcgg ccttctcggc gttcctgtcg acgaggatca gggatgacag
     8281 gaggtcgggc cgcttctcca gcgcgggaag gtagaacatt tcggcgatcg cgccgcagcc
     8341 gatgagcgcg atgggcagat cgggcatggc ctcgagtcgg tacgtgtcgt gggcaaggcg
     8401 cgcggaggaa cacgggcggc cgaaggatac ccgtgaacca gcggtcagtg cgactggcgg
     8461 cgcgggccca ggtgaccaac cacgaccgcc accgtctgca ggatcagctc cgcgtcggtc
     8521 cgcgccgtcc gcctggcgag gtagtccagc tcgagctgga gcttggccgg catgatcgtg
     8581 agccggtagg tggcctccca gtcctcgccc tgcaggagcg actcctcgtc cgcgtacgcc
     8641 agggcggcga cgcccgtgat gcccggcttg accgagagca cgcgccgctg ctcgtcggtg
     8701 tagatggcga cgtagctcgg atcctcggga cggggaccca cgatgctcat ctcgccgcgc
     8761 aggacgttga gcagctgcgg cagctcgtcg agcttgagcc gccgcagtcg ccgccccagc
     8821 ggggtgaccc gcgggtcggc ccagacggtc accgacgagc cgccggccgg gaccatcgtc
     8881 cggaacttga acagacggaa ctcgcgacca tgctggccca cccggcgggc cttgtagatc
     8941 accggcgccc cgtcacgcag gcgcacggcg gcggcgatcg cgagcaggag cggcgccaac
     9001 acggccagcc cgatcagggc gagcacgatg tccgtcagcc ttctagccat cggcgggggt
     9061 cgtcccggca gccctccctg accgtcctca cccagctccg gctcgcgcgg gacccgggaa
     9121 gctcacctgc tactatcccg actcgcgtct tggcgcgggt cgcgaccggg gagagcatga
     9181 cttcgtgagc acggttcttg ttgccggcgg tgccggctat atcggctcga tggtatcccg
     9241 cgagctgctg gccaggggcc accgcgtcgt cgcggcggac gccctcctct ttggcggcga
     9301 ggcgctcctc gacctgctct cccacccgga cttcgcgttt cgcaaggtcg acgtccgcga
     9361 cccccaggac ctggaggcgc tgttctccac ctggcagttc gatgcggtgg tccatctcgc
     9421 cgcgatcgtc ggcgatccgg cctgcaaggc gcagccggag ctcgccaccc agacgctctg
     9481 ggacggctct cgaagcctgc tcgagctcag cgatcagcac ggcgtgggcc acttcgtgtt
     9541 cgcctccacc tgcagcaact acggcaggat ggagtccaac gccgtcctcg atgaggacgc
     9601 accactccgc ccggtcagcc tctacgcgga gctgaaggtc cggttcgagg agctcctcat
     9661 ggcccgatcg gtggccctgg acttcaccat cctgcgcttc gccaccgtgt acggcctgtc
     9721 gctccggccg cgcttcgacc tgaccatcaa cgagttcgct cgcgacgccc tgctcaaggg
     9781 cgagctggag gtcttcgggc cggatttctg gcgaccctac tgccacgtgc gcgacatcgc
     9841 gaacgccgtg accatgatcc tggagcagga ccgggggcgg gttgccggcc gggtcttcaa
     9901 cgtcggcgcc gacgacgaga actaccagaa gcgggcgatc gcggaggccg tcgccgagca
     9961 gatccccgtt cgtctccgct acgtcgaacg ggccgaggac ccacgggact accgggtctc
    10021 cttcgagcgc atcgccgccc tcgggttcac gccccggatg cgcctgcgcg acggcatccg
    10081 ggaggtggca gaggccatcg ccacgggcct gatcgcggac ccctacgcgg cccggtaccg
    10141 caactcttga gccgaggacg ctgatgatcc cgctgtccga gccgtccctt ggtgggcgcg
    10201 aactcgaata catcacccgc tgcctggaca cccgctggat ctccgcgaac ggcgagttcg
    10261 tgtgggagat ggagaagttc ctggcgagct atctcggtgt gaagcacgtc gtcgcctgca
    10321 actcgggcac gtccgcgatc cacatctcgc tcatgttgtc gggcgtccag cccgacgacg
    10381 aggtcatcgt gcccacggtg acgttcatcg ccccggtcaa cgcggttcgc tacgtgcacg
    10441 cctggccggt gttcgtcgac tgcgacgagt tctgcaacat ggacgtcgag ggcgtgcgga
    10501 gcttcctcgc cgacgagtgc gtcgtccgtg acggcgtcct gaccaaccgc actacgggcc
    10561 ggcgcgtctc cgccatcgtg ccggtccacg tcttcggcac ctcggtcgac atggacccga
    10621 tcctggcact ggcagaggaa tacggcctcg cggtcgtcga ggacgcgagc gagagcctgg
    10681 gctcgcgcta caagggtcgg atgtgcggca ccctcgcgcc catcagctgc ctgagcttca
    10741 acggcaacaa gatcgtgacc accggcggcg gtggggcgat catgaccgac gacgacgaga
    10801 tcgcccgcca ggcccggtac ctgaccacgc aggccaagga ggacggcctc gagtacatcc
    10861 acaacagcgt cggctacaac taccggatga acaacgtcct tgcggcgatc ggtctggcgc
    10921 agctcgagga tctcgacgag cggctcgcga tcaagcgcgc caacttcgcc cgctatcagg
    10981 aggcgctcgg cccgtcccgc ctcctcgacc agcccgagtg gagcgaatcg aaccgctggt
    11041 tctacgccct cctgtgcgac agcgcggaca tgaagcgcga gcttctggag gcctgcacgg
    11101 cggccggggt ccaggtccgc ccgctctggt acctcaacca cctgcagcag ccgtaccgcg
    11161 agacgcaggc gcaccgcatc acgcgtgccc accacttcta tgacacggtg ctcaacctgc
    11221 cgtgctccat ctcgctgacg ccgcagcaga tcgcggaggt ggtcgccctg gtggaggctg
    11281 cgaagcgcgg cgcccctgag gacgccatcg cacggacccg ggcctgagca cggagatggg
    11341 cgggcggatc cccacgatca ccgtcggcat cgccacgctc gatcggtacc cgtacgtcgc
    11401 cgacctcctg gacgacctgg cagcgcagac ccacctcccc gacgaggtgg tgatcgtcga
    11461 ccagacagcc ccccacaggc ggcagccatt cgagacgacc aggtggagcc atgcgttccc
    11521 gatgcgcgtc gtggtccagg agacgcgcgg caccaccaag gcccggaacc ggctgctccg
    11581 ggagtgtcga tccgaggtca tcctccagcc cgatgacgac agccggatcg accccgatta
    11641 cgtggagcac cacgtccagc actacgccga cgaccgggtg gatgtcgtga gtggcccggt
    11701 gtacgaatgg gatgccgaca cccgcgagtg gtacgtcaag tggagcaacc acctgatgat
    11761 cggggggtgg aacggccccg cgttcgcgag cggccattcc ttcgccgggg gcaacagcag
    11821 catccgggtc gcgagcgccc ttgcgatcgg cggatgggac gagaacatcc tgacccacgg
    11881 ggaggacgag gacctctcgg accggctgct cgcagcggat gccgtgatcg tgtacgaccc
    11941 caaggctcgc ttgctgcacc tccgggcgcc gagaggcggc gagcgtgctg cggagtgggg
    12001 cagcgggcct gatggcaact ggcgggtgct cgccggctac ctgtactact tcctggcaaa
    12061 ccggccgccg tacgacgcct gggagatgtt ccgcgagtac accatccgcc ccttctggca
    12121 catcaagcac cgtcgcacat cggtcggctt gaggctcctg ccgcgcgtcg ctgcggcgat
    12181 cccggtctcc acgtggcggt gggttcgtgg tcgtcggctg atcgaccccg cgagccctga
    12241 ccgggaggtc cggcggatcc ccaagaagga ggtggcgcag tggcgccgga ggttggcagc
    12301 caagcgacct cgaaccgtcc tgcccgaccg agacccaccg cagcttccgc tgtagccccc
    12361 ggcctcaccc aggccggcct caggaggact gcggtcgcct ggccgaccgg atcatcggac
    12421 ccaggcacca gcactgcggc tcacagcccc agcaggttcg cttggcgatc gaattgcggg
    12481 accacagctc ggacttcctc gaaggtcccc tgtgccacca agcggccgcg gtcgaggtag
    12541 atgaccgtgt ctgcgcgccg gatggtggcc agacggtgcg cgatggtgat cgtggtcacc
    12601 tctgacccca gttcttcgat cgtctgcgtc accaggttct cgatctctgc gtcgagggca
    12661 ctggtcgcct cgtccatcac aagcagccgt gggtcggtgt acagcgcgcg agcgatcccg
    12721 agccgctgcc tctggccacc gctcaactgc acaccacgct ccccgacttc ggtgtcgagt
    12781 ccatcgcggg cgtcggccag gtagtcagag agatgagctc gcttcaatgc agcccagact
    12841 tggtcgtcgt tgacctcgtc ccgcgtgcgt ccgagcgcga cgttgtcccg caccgagccc
    12901 gagacgaggg cgacggactg cggaacgtag gcgaccaggc cgggatagcg gcgcacgacc
    12961 tgcccgggag acagtccgcc gatccggaca agacccgccg agggctgcac caccccaagg
    13021 atggcatctg cgagggtgga cttgcctccc cccgaagggc cgacgactgc aagggctccg
    13081 ccgggtggca gggtgaacgt gatcccctcg atggcagggg catcagagcc cgggtagtcc
    13141 accttcaggt cccggacagc gacctcaaga acgaggtcgg gcttgccggg acgcccggcg
    13201 ccgacctcgg ttgcaggcgc ggccgggggg gtggtctcca atatggccgg tgtcgcgtgc
    13261 tcccggatgt gggcggccat cgtgaaggcg taatcggcgc ggggcaccag ggaccgcaag
    13321 gtgatccggg aaccgttcag ccggagcaag gagggcatga cacgggtcgc ggcggtgagg
    13381 aacagcgcga gggagccaag cgccgattcc acggtgtcgt gctggagcag tgcgaggacc
    13441 agcaggcctg cgccgacgac gagggcgatc tccatcccgt agcggggcac ctggctgatg
    13501 aactgctggt cggcgtaggc ttctgagcct gcctgccgga gcgagcggaa ccggtcgatg
    13561 tagtgctgtc gacgatccgc cacggtgatc tctcgatacg tcgcgatccc gtcctggacg
    13621 gtcacattgg actcccagga agactcagcc atgaccttgc cggttgtgta ggcccagccc
    13681 ctgaggacct ggttcatgag gaccgcgatc aggccgaagt acccgacggc gaacagggcg
    13741 atgagggggt tgagcaacag gagggccccg ccgaggacga caaggaggct gagctccacc
    13801 cagatgacca tcgcgttggt gagggtctca acgattgctc cgctaacgcc gtgcaccagg
    13861 gcgaaggctg accattggct ttcgaagcgg ttgacatgca cgatcggaag cgcgtagaag
    13921 tccgcgcaca tctgtgcaga gatacgggtt gacgccgtcg tgaggaacct cgtggtccag
    13981 cgcaggatcc acagggaggc gacggtcttg aggaccagca gcaaggctgc gattcccgcg
    14041 agcacaaggc tcgccgtggt agctggaaca tcggccaggc caaaccacga cagcacctcc
    14101 tcgaccgcgt ccgggagcgg gacgccctgg gcggcggacg ccgcaatgac ccccaccatc
    14161 ccgaacaaca gcaccccggc cagatccaac agggatgtcg ccatctgaag tccgacggcc
    14221 acccagtagt tgcgtcgctc cctgcggctg agcatcgcaa gcgaacgtct gacctcacgg
    14281 ccgatcaacg ggttcgccct ggcctccgag acggtcgcct gcgtcacggg cacatcacgc
    14341 atggggttgt tcgcgaccct gcgacaactc gttgccttcg ggacgaacgg gtgagagggc
    14401 gccgcccggc gagtgctgtc gacgaacgac gagccccacg gccgtcccac gtgcggctga
    14461 ccgcgcccag cacgatgtcg cgtccctcca ctagcacgtg gccccggaac gggcgtgcgg
    14521 tgcacgtgcg cgcatacggt tgtccgacac agcggaatgc atcacccagc tcctcgatct
    14581 cccgaagcac tatatgcgag acggtcgctc cggtccgcgc cgggcgcggt gatgacgagc
    14641 cggtcgcctc catgcccgtt ccgccgaagt caggagaggc gacgcaccgg ctcgtcaaca
    14701 cacaggtgac acgctgcgtt gggcggagaa aggaggaccc atccgatgct cgaacccatg
    14761 gcgccgcgcc cggaggtgag cgatgctggc gccatgcccg atcccgaagt ccgcgagcgg
    14821 ccgattcgcc ggagccgtac ggtcgtggtc ctcggcggag gcggccatgc caaagtgctg
    14881 atcagcgtcg tccgcaagct gccctggacg atcctcggct acgtggatcc gcgcgacgtc
    14941 gggcccgtgc ttggcgtccc ccatgccggc ggcgacgatg tgctgccggc cctcctcgcc
    15001 cgcaaccccg gttgtgcggc ggccatgggt gtcggcaagg tggacgcgac ggcgcgtcgt
    15061 gccgggatcc aggccgcagc cgaagcgttg gggtaccgat ttcccacctt cgtctctccc
    15121 gacgccctgg tcaacgacga ggtagagctg ggggccggca cggccgtctt cgacggggcc
    15181 gtcctcaatc cgggcgtgat caccgggccc acctgcgtcg tcaacaccaa cgccaccgtg
    15241 gagcacgact gccagttcgg cacgaacgtc cacatcggcc ccggcgcgac cgtgagcggc
    15301 ggggtcacga tcggcgacca caccttcgtc ggtgccggag ccgtggtcat ccacggcgta
    15361 cggatcgcgg aggcctgcat gatcggtgca ggagcggtgg tcacgggcga cctgaccgag
    15421 ccgggcacgt acgtcggggc tcccgcccgg aggatccgat gagcgccggc agcgccctcg
    15481 ccgtgattcc cgcccgcggc ggatcgaggc gggtaccgcg caagaacatc cgcgagatgc
    15541 atggccggcc gctgctcgcc tactcggtcg aggccgcgct gcgcagtagc gcctttgccc
    15601 gggtcgtcgt gtcgaccgac gatgccgaga tcgcggccgt ggcccgggcc gcgggcgcag
    15661 acgtcccgtt tctccggtca cccgcccttg ccgacgacgt gacgccggtg tcgcgggcga
    15721 cgatcgacgt cgttgatcgc ctggaggcgg acggcgagtc gtacctcgag gtctgccagc
    15781 tgatgccgaa ctgtccgctg cgcacggccg acgacgtggt ggccagccat cgccagttca
    15841 ccgaccagcc ggccgaggcg cagatctcgg tcgcgcggta cggctggcag agcccgtggt
    15901 gggcgatgcg ccgggcggcg gacggctcgc tggaccccct cttcccggat gcgatgcgcc
    15961 agcgcagcca ggaccttccc gagctcctct gccccacggg agccgtctgg tgggccagga
    16021 cggacgccct gcgtcgcgag gggacgttcc accttgccgg caggaccggc tgggagatcg
    16081 actggacgca cgcgatcgac atcgacaccg aggaagactg gcggctggcg gaggcgctga
    16141 tggggcgttc atgagcgcga tggccctcta caccgccgtc taccccggtg ccgcccgctt
    16201 ccttgcgtcc tggtacgcgt cggtccaggc acaggcggac cgcgacttcg atctctggat
    16261 cagcctggac gggctgaccg aggcggcggt cgttgcgcgg ctgggagatc gcccggccgc
    16321 gaccttcgtc gttgccgaag cggggttcac gcccgcccag gtgcgccagc aggcgctggc
    16381 ccggatcgcc gaccgctacg acgcggtggt cctcgtcgac agcgacgacc tcctgcaccc
    16441 ggatcgcgtc gctgcggcac gtgcgtggct ggaaggcgcg gatgtggccg cctgcggtct
    16501 gcgcctggtc gacgaggccg gacagcccct cggggtgacg ctcgcgactc caggcgaggg
    16561 ggaggtcgat tcgatcctgc cacgcgccaa tgccttcggg ctctcgaaca cggcctaccg
    16621 gtccgatgtg ctgcgatcct gcctgccgat ctcggccgcg gccgccctgg tggactggta
    16681 cctggtcacg atggcctggc tccagggcgc acgactggcc ttcgatccga ccccccgaat
    16741 ggactatcgc cagcacggcg caaacatggc caggatcaga tcgccattca cggcagagcg
    16801 agtgcaagcc gacacggcgc tggtccgcga gcacttccgg ttggtgctgg acggcgtgcc
    16861 ggcgggagcg ctgccggcgc gggtcacgca ggtgcgcgaa gccgccgccg aggtcgctac
    16921 attcgaccag cgcgtcgtcg aggactcggc cgcactcgcc atctacgtcc gggacctgaa
    16981 tgccgcgcct ccgcccctcc tctggtgggc gtccgtcgcc caccctcccc ttcgattcca
    17041 gtggagccct cagaaggagc caacgtgaga cccgtcaacc tgggacctgt ggccgtgggc
    17101 ggcgacaacc cgccctacat cgtcgcggag gtcggctcca accacaacgg ggacatggat
    17161 ctctgcatgc ggctgatcga cgccgcggcc gaggcgggcg ccgacgcggt caagttccag
    17221 tcgtggagtg agacgtcgct catcgcccgc gaggaatacg agcggaatcc cagctactcg
    17281 gacacgaaga agcacttcgg gtcgctccgc gagatggtgc gcgcgtacca gctcacgccc
    17341 gagcagcacg tcgtcgcccg cgatcacgcc cgggagcgag ggatcgcctt cgcctccagc
    17401 gcgttcgccc cggcggaggt ggacctgctc gccgggctgg acgtcccgtt catcaagatc
    17461 gcctcgatgg acatcaacaa cctgccgctc ctggcctacg ttgcccggtc gggcaagccc
    17521 gtcgtgatct cggtgggcat ggcctccctg ggcgagatcg agcgagccat ggacacggtc
    17581 cgtgccgagg gcaacgagga catcgtcctg ctccattgcg tctcgatcta cccggcgccg
    17641 ccgtcgaccg tccacctgcg caacctcgag accctgcgca acgccttcga cgttcccgtg
    17701 gggttcagcg accacaccct gggcacggcg atcccgctcg ccgccatcgc cctgggcgca
    17761 tgcctgatcg agaagcactt caccctcgac agggagatgg agggctggga ccacgacatc
    17821 tcgtccgatc ctccggtgct gcgggccctc gtcgaggagg ggcgcatcat ccacgaggcg
    17881 ctgggcagca cgcgccggat gctgtcggag gccgagatcg tcaagcgccg ccagttccgg
    17941 cgcagcctgg tcacgcggac cgcgctgccg gccggtcaca tccttgccga ggcggacctg
    18001 gacgcgaagc gcccgggaac cggcatcagc cccgacgagc tccgctatac cgtcggccgc
    18061 gagctagccg cggacctcgc cgaggaccag gtgcttcgct gggagcacct gcgctagatt
    18121 caccctctcc cccctgagga tccagatggc acgccccgtc tacgtcgtcc actgcgtgga
    18181 cacggaggga ccgctccacg aatcggtcga ggcgaccttc gaacgcctcg aggccatctt
    18241 cgggatcgat ctcgagccca gcgtcgcgct gctccggcag ctccaagccg gcgaggtcga
    18301 cctcggcggc atcgagcagg ccgtgcgcaa ggtcgtggac ccccacctgc tggcctacaa
    18361 cgacacctgg gacaaagtca acgagatgct cgcgtcgtgc atgtc
//
LOCUS       DBNHXO010000265         6388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2255800, whole genome shotgun sequence.
ACCESSION   DBNHXO010000265 DBNHXO010000000
VERSION     DBNHXO010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2255800"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="AC162A_05540"
     CDS             <1..274
                     /locus_tag="AC162A_05540"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HIR5010338.1"
                     /translation="RNARAMTEPDGVFLLVWVSTPLAVCEARDRKGLYARARAGEVAE
                     FTGISSPYEPPEDADVVIDASEVGVPEAVRRIREALDVALEDPARR"
     gene            complement(306..1502)
                     /locus_tag="AC162A_05545"
     CDS             complement(306..1502)
                     /locus_tag="AC162A_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010339.1"
                     /translation="MPEGSLLVHIGPHKTGTTALQAALWRGRAALLEQGVRHIGQSRN
                     PSNAVRAVTGQSSPYAVDTPPAMRYWHSLVKEANAAHEPRRVISSEFFAWAKPDVIRG
                     IVDDLGGERVRIAVTLRSLGRVMPSMWQQNIQAGTVAEFDAWLRQMLERPPDKPPAPF
                     WTLHRHDQLIARWADAVGTDRVTVIVADDSDHAFVLRVFEEMLGLRDGTLEMHRDLTN
                     RSLTLPEAEAVRAFNVAFKAEQLDRALHARVMRFGAAQLMKEREPSPDEPRVEMPSWA
                     LHHVDRISRAMVDGIVASGVRIVGNVERLTDRPPSRSDDAPGVVPVPPSVAAAMTMGV
                     LVATGATRGAASRIGGPQFAEPVELLRVPTYQVIGVVLIRTRRSIGARLVAIRRRVLP
                     KRKAKD"
     gene            complement(1585..2859)
                     /locus_tag="AC162A_05550"
     CDS             complement(1585..2859)
                     /locus_tag="AC162A_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010340.1"
                     /translation="MTGPIAEEKTSLDPATRPSPTSGAAGAPPTDEVQLPEGTRLLHI
                     GPPKTGTTSLQSALYAARPALSRAGVHHAGRARNPAAAAQAVTGRTSMYLGGVVPPMS
                     RWERLVKDVRRAEEPRVIISSEFFADATPEAIRRIVHDLDPDRIHIAVTVRPLASILT
                     SQWSQYVADGMGAPFERWLEATLTPDDHRYSPSFWIRHRHDTLVARWADVVGPERVSV
                     VVVDAERGAVLRAFERLLGLPRDMLVADPDLANRSLTLPETEAVRAFNVQFKRAGLPK
                     PLLARVMHFGATRAMKLAPPDPTWPAAELPAWSLPETRRIAREIVDNIAGSGVRVIGD
                     LESLAHVPEAPPGDTPTADVPGPPQIAAGMAMGVLLATGEARRDSDITVETRDRELER
                     VPLFVLGTAIAIRARDAARRRVRSLRRLGRVS"
     gene            complement(2856..4037)
                     /locus_tag="AC162A_05555"
     CDS             complement(2856..4037)
                     /locus_tag="AC162A_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010341.1"
                     /translation="MEATADLAALRLPEGTRLLHIGPPKTGTTTIQAAFHAEGRALAS
                     LGVHYAGRTRHSRRAVFAVTGRRWLGGEPPPITEWTSLVAEIRAASAPRVVLSSEAFA
                     QADESQIRRVIDDLDAGRVHVVVTLRPLASILPSSWQQSVQTGNDTAYPDWLAAQLDD
                     DRTTSPRSFWYRHRHDLLIARWAEVVGPERITTIVLDGSDRSRVLRVFEGLTGLPAGT
                     LVENQDLTNRSMTGPEAEAVRAFNAAFWRAGLSLRLHNRVMHLGAAERLKLHEPRPEW
                     PRIETPQWALDRAAIVAREIVAGIRASGIRVVGDLDRLADTPAGTVDAARDGDRIPSD
                     IAAEMAVGIVFAGGLAVEDRPSPSRDLTEVPSRVMARALARRAWTAATRPVRSMKGSK
                     R"
     gene            4126..5352
                     /locus_tag="AC162A_05560"
     CDS             4126..5352
                     /locus_tag="AC162A_05560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5010342.1"
                     /translation="MTIEIRTARPDEYADYLESLSTAFLDRLDLEVVSSVVAQTWDPA
                     RTWAAFDGDRICGTFRSWATEMTVPGGATLPASAVAGVTVLPTHRRRGILTSLAAAEH
                     GAIRERGEAFGLLYASEWPIYGRFGYGAGCRVATWTIQASRTSVHGTPTGGVELVRPD
                     AAAADTIKSVFETWRIRQAGELRRRDFRWTDDLGLRDTGFGPPWKGFLAIHRDGSGAA
                     DGYARYTAEEKFVDRQPASQIRLNELHALNEPASTALWRYLLEVDLVTTVRAEGRSPS
                     DRLPWLLTNARAAIPGEIGDGLWVRLFDVPRALAARTYEREATVVIEVVDAELGAPLR
                     VLLDASTDGATCTTSGRTPDLTLPVAALGSAYLGGTRLRDATIGTGVDEHTPGSLATL
                     DAMLRTTDEPWCSTFF"
     gene            complement(5363..5998)
                     /locus_tag="AC162A_05565"
     CDS             complement(5363..5998)
                     /locus_tag="AC162A_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010343.1"
                     /translation="MDRLLIGLIAIGVGALVAAYGARGFFILLPLFGFVVGFGVGAQI
                     VANLFGDAFFATITSWGVGLAFAVVFAVLAGLWWWAAVVILFGVVGYELGSGVLIALG
                     LDPGLITFIAGLAVGIALAILALVLDAPTMLVAAVSAFGGAAYVVAGLYLILDQITID
                     QLEDGPLGALDGRPVALVAWVALGAVGLLFQYYDTRRVGIEAIERSRYRFS"
BASE COUNT          946 a         2288 c         2293 g          861 t
ORIGIN      
        1 ccgcaacgcg cgggccatga cggaacccga cggcgtgttc ctgctcgtct gggtcagcac
       61 gccactggcc gtctgtgagg cgcgggaccg caagggcctc tacgcccggg cgagggccgg
      121 cgaggtcgcg gagttcaccg gcatctcctc gccctacgag ccgcccgagg acgccgacgt
      181 ggtgatcgac gccagcgagg tgggggtgcc cgaggcggtt cggcgcatcc gggaggcgct
      241 cgacgttgcg ctcgaggatc cggcccggcg ctgatcgccg cccgggacac tgccaaggaa
      301 acggttcagt ccttggcctt gcgcttgggc agcacccggc gacgaatggc gaccagccgt
      361 gcgccgatcg agcgtcgcgt ccggatcagg acgacgccga tcacctggta ggtggggacg
      421 cggaggagct cgacgggctc cgcgaactga ggtcctccga tgcgcgacgc cgcaccccgg
      481 gtcgcgcccg tcgccacgag cacgcccatc gtcatcgcgg cagcgacaga cggcgggacc
      541 ggcacgacgc ccggcgcatc gtcgctgcgg ctcggtggcc ggtcggtcag ccgttcgacg
      601 ttgccgacga tccgcacccc cgaggcgacg atcccgtcga ccatggccct cgagatcctg
      661 tcgacatgat ggagcgccca tgacggcatc tcgacccgtg gctcgtcggg cgacggctcg
      721 cgctccttca tgagctgggc cgccccgaag cgcatgacgc gggcgtgaag cgcccggtcg
      781 agctgctccg ccttgaacgc gacgttgaac gcccggacgg cctcggcctc gggcagcgtc
      841 agcgaccggt tggtcaggtc gcggtgcatc tcgagggtgc catcgcgcag cccgagcatc
      901 tcctcgaaga cccgcagcac gaacgcgtgg tcgctgtcat cggcgacgat cacggtgacc
      961 cggtccgtgc cgacggcatc ggcccagcgc gcgatcagct ggtcatggcg atggagcgtc
     1021 cagaacggtg ccggcggctt gtcgggcggc cgctcgagca tctggcgcag ccatgcatcg
     1081 aactcggcga ccgtgcccgc ctggatgttc tgctgccaca tcgacggcat cacgcggccc
     1141 agcgagcgaa gcgtgaccgc gatgcgcacc cgctcgccac caaggtcgtc gacgatcccg
     1201 cggatcacgt cgggcttcgc ccaggcaaag aactcgctgc tgatcacgcg gcgcggctca
     1261 tgggcggcat tggcctcctt cacgaggcta tgccagtagc gcatcgcggg cggcgtgtcg
     1321 accgcgtacg gcgacgactg cccggtcacc gcgcgaaccg cgttcgacgg attcctcgac
     1381 tggccgatgt ggcgcacgcc ctgctcgagc agggccgccc gcccgcgcca cagcgcggcc
     1441 tggagcgccg tcgtgcccgt cttgtgcggc ccgatgtgga cgagcagcga cccctcgggc
     1501 acgagcaggg acgccccgct cgcgagcgtc ggacccgaac tggtcagttc cgtctccctt
     1561 cgaatcccgg cacgtcctgc ccggctacga tacccggccc agacgccgca gcgaacgcac
     1621 gcggcggcgc gccgcgtccc gcgcccggat ggcaatcgcc gtcccgagga cgaacagcgg
     1681 aacgcgctcg agctcccggt cccgggtctc cacggtgatg tccgagtcgc gccgtgcctc
     1741 gcccgtcgcg agcaggacgc ccatggccat cccggcggcg atctgcggtg ggcccggcac
     1801 gtcggcggtc ggcgtgtcgc ctggaggcgc ctccggaacg tgggcgagcg actcgaggtc
     1861 gccgatgacg cggacaccgg agccggcgat gttgtccacg atctcgcgcg cgatgcgtcg
     1921 ggtctcgggc aacgaccagg cgggaagctc cgcggccggc caggtcgggt cgggcggcgc
     1981 gagcttcatc gcgcgcgtcg ccccgaagtg catcacccgc gccagcagcg gcttgggcag
     2041 gcccgcgcgc ttgaactgca cgttgaatgc gcgcacggcc tcggtctcgg gcaacgtcag
     2101 cgaccggttc gcgaggtccg ggtcggccac cagcatgtcg cggggcaggc ccagcagccg
     2161 ctcgaacgcc cgcagcaccg ccccgcgctc ggcgtccacg acgacgaccg acacgcgctc
     2221 cggtccgacc acgtcggccc agcgggcgac cagggtgtcg tgccggtggc ggatccagaa
     2281 ggacggtgaa tagcgatggt cgtcgggggt gagcgtcgcc tcgagccagc gctcgaaggg
     2341 agcgcccatg ccgtccgcga cgtactgcga ccactgcgac gtcaggatcg aggcgagcgg
     2401 gcggaccgtg acggcgatgt ggatgcgatc gggatcaagg tcgtggacga tccggcggat
     2461 ggcctcgggc gtcgcgtccg cgaagaactc gctgctgatg atcacgcgcg gttcctcggc
     2521 gcgccgcaca tccttgacga ggcgttccca gcgcgacatg gggggcacga cgccgcccag
     2581 gtacatcgac gttcgcccgg tcaccgcctg ggcggcggcc gccgggttgc gcgcgcgtcc
     2641 cgcatggtgc acgccggcgc gcgacagggc cggccgcgcg gcgtacagcg ctgactgcag
     2701 cgaggtggtg cccgttttgg gtggcccgat gtgaagcagg cgggttccct cgggcagctg
     2761 aacctcatcg gtcggtggtg cgccggcggc cccgctcgtc ggcgaaggtc tggtcgccgg
     2821 atcgagagac gtcttctcct ccgcgatcgg ccccgtcacc gcttggatcc cttcatcgag
     2881 cggacgggcc gggtcgcggc ggtccaggcc ctgcgggcga gcgcacgggc catgacgcgc
     2941 gagggcacct ccgtaaggtc gcgcgatggc gatggccggt cttcgaccgc gagcccgccc
     3001 gcgaacacga tgccgacggc catctcggcc gcgatgtccg aggggatccg gtccccatcg
     3061 cgcgcggcat cgaccgtccc ggcaggcgtg tcggccaggc ggtcgaggtc accgacgacc
     3121 cggatgccgg acgcgcggat cccggccacg atctcgcgcg cgacgatggc cgcgcggtcg
     3181 agggcccatt gcggcgtctc gattcgcggc cactcgggac gcggctcatg gagcttcagc
     3241 cgctccgccg ctccgaggtg catcacccga ttgtgcagcc gcaggctcag gccggctcgc
     3301 cagaacgcgg cgttgaacgc tcgcacggct tcggcctccg gcccggtcat cgagcggttg
     3361 gtcaggtcct ggttctctac gagcgtgccc gcggggaggc ccgtcaggcc ctcgaagacg
     3421 cgcaggacgc gcgaccgatc cgagccgtcg aggacgatcg tggtgatccg ctcgggcccg
     3481 acgacctcgg cccagcgggc gatcagcagg tcgtgccggt gtcgatacca gaacgaccgt
     3541 ggactcgtcg tccggtcgtc gtccagctgc gcggcgagcc agtccgggta ggcggtgtcg
     3601 ttgcccgtct ggacggactg ctgccacgac gacgggagga tgctggccag cgggcgcagg
     3661 gtgacgacga cgtggacgcg gccggcgtcg aggtcgtcga tgacccgccg gatctgggac
     3721 tcgtccgctt gggcgaaggc ctcgctgctg agcacgacgc gcggtgccga cgcggcgcgg
     3781 atctccgcga cgagcgaggt ccactcggtg atcggcggcg gttcgccgcc cagccaccgc
     3841 cggccggtca ccgcgaacac ggcgcgccgc gagtgacgcg tccggccggc gtagtggacc
     3901 cccagcgagg cgagcgcccg accctcggcg tggaaggccg cctgaatggt cgtcgtgccg
     3961 gtcttcggtg gcccgatgtg gagcaggcgc gtgccttcgg gaaggcgaag cgccgcgaga
     4021 tccgctgtcg cctccatggc ctccgctcct gggtcctcga cgcggcctac gatacccgcc
     4081 agccccacct tccggagcca cccggtcggg atgtagagtc gcgcgatgac gatcgagatc
     4141 cgcacggcgc ggcccgacga gtacgccgac tacctcgagt ccctcagcac ggcattcctc
     4201 gatcggctgg acctcgaggt ggtctcgagc gtcgtcgcgc agacctggga tccggctcgc
     4261 acatgggcgg ccttcgacgg tgaccggatc tgcgggacgt tccggtcctg ggcgaccgag
     4321 atgacggtgc caggcggggc gacactgccc gcgtcggcgg tcgccggcgt cacggtcctg
     4381 ccgacgcacc gccggcgcgg aatcctcacg tcgctcgcgg ccgcggagca tggggcgatc
     4441 cgcgagcgcg gcgaggcgtt cggcctgttg tacgcgtccg agtggccgat ctatggccgg
     4501 ttcggctacg gcgcgggatg tcgggtcgcg acgtggacga tccaggcatc gcgaacctcc
     4561 gttcacggca cgccgaccgg cggcgtcgag ctcgtgcggc cggatgcggc ggccgctgac
     4621 acgatcaagt ccgtgttcga gacctggcgg atccggcagg cgggcgagct ccgccgccgc
     4681 gacttccgct ggaccgacga tctcggcctg cgcgacacgg gcttcggtcc gccgtggaag
     4741 gggttcctcg cgatccatcg ggacggatca ggcgccgccg acggctatgc gcgctacacg
     4801 gcggaagaaa agttcgtcga tcgccagccg gcgagccaga tcaggctcaa cgagctgcac
     4861 gccctgaacg aaccggcgag cacggcactg tggcggtacc tgctcgaggt cgacctcgtc
     4921 acgaccgtga gggccgaggg ccgcagcccg tcggaccgcc tcccgtggct gttgaccaac
     4981 gcccgcgcgg cgatcccggg cgagatcggt gacggactgt gggtccggct gttcgacgtc
     5041 ccgcgtgcgc tcgccgccag gacgtacgag cgcgaggcca ctgtcgtgat cgaggtcgtg
     5101 gacgccgagc tcggggcccc gctgcgggtc ctcctcgacg cgtcgaccga tggagccacg
     5161 tgcaccacct ccggccggac gccggatctc accctgccgg tcgcggcgct gggctcggcc
     5221 tacctcgggg gcacgaggct gcgcgacgcc acgatcggga ccggggtcga cgagcacaca
     5281 cccggatcgc tcgcgaccct ggacgccatg ctccgaacga ccgacgagcc gtggtgctcg
     5341 acgttcttct aggcgctcgg gatcagctga agcggtagcg gctccgctcg atcgcctcga
     5401 tcccgacccg gcgggtgtcg tagtactgga agaggagccc gacggcgccc agggcgaccc
     5461 acgcgacgag cgccaccggg cgcccgtcaa gcgcgccgag cggcccgtcc tcgagctggt
     5521 cgatcgtgat ctggtccagg atcaggtaca gcccggcgac gacgtacgcc gcgccgccga
     5581 acgccgagac cgccgcgacc agcatcgtgg gcgcatcgag caccagcgcg aggatcgcga
     5641 gcgcgatgcc cacggcgaga ccggcgatga aggtgatcag cccgggatcg aggccgaggg
     5701 cgatgagcac gccgctgccg agctcgtagc cgacgacgcc gaacaggatc acaacggccg
     5761 cccaccacca caggccggcc aggacggcga acacgacggc gaaggcgagc ccgacgcccc
     5821 agctcgtgat cgtcgcgaag aaggcatcac cgaacaggtt ggccacgatc tgcgcgccga
     5881 ccccgaaccc cacgacgaag ccgaacaggg gcaacaggat gaagaatccg cgcgcgccgt
     5941 aggcggcgac caacgcgccc acgccgatcg cgatcaaccc aatcagcagt cggtccaccg
     6001 gatcctccag ccctcctcga tgcgccccca tcccgggagg ccgcccggcg atgatggcac
     6061 aacgcctctg gcgatcggag gcccgatcgg gctcgcattg acgattcggg ccggaattcc
     6121 gataattcca ctcgggatta gcccgcggcc cggctttcgg cggtcgcggc gtcccggatc
     6181 acgacccacc cccgcggtcg accgaccgtg actggagcag cgatgacctc gacccgcgac
     6241 ctcgtccgtg acgaacgcga tcagttcgcc ttccacgacg acatcgtcta cctgcgcgag
     6301 accaagcgtg ggctgacgcg cgacacggtc gaggagatca gcgggttcaa gggcgagccg
     6361 gactggatgc tccagttccg cctgcgcg
//
LOCUS       DBNHXO010000266         2452 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1263833, whole genome shotgun sequence.
ACCESSION   DBNHXO010000266 DBNHXO010000000
VERSION     DBNHXO010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1263833"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="AC162A_05570"
     CDS             complement(<1..934)
                     /locus_tag="AC162A_05570"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5010344.1"
                     /translation="MSSTLPPAGSPTSSRDEVTSDRPDAYIPRDRRWALHAGHTLPDR
                     VHGSALFADISGFTPLTEALAKELGPQRGAEELTANIGRVFHAVISELDAYDGNVIYF
                     AGDAITCWIDGDDGTRACAAAEGMQQAIGRTGTIVTPGGSTVELGMKVAIAVGDARRF
                     VVGDPDIQLIDVLAGRLMDDLAEAEHHAQRGEIVLDASALAALGDRVATAELRRDEGA
                     GGACGVFGGLLVDVEPEPGHQPEPLPEDLVRPWLLPAVYERLRTGRGEFLAELRPAIP
                     VFLRFGGIDYDHDDDAIGKLDEFVRAAQRVLNGYG"
     gene            complement(972..1745)
                     /locus_tag="AC162A_05575"
     CDS             complement(972..1745)
                     /locus_tag="AC162A_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5010345.1"
                     /translation="MSGFRVLLRKELAEAWQTRRLPIVAGLFAVIGMISPLTARYLPE
                     IMQAALGDQLPVALPAPTAAMAVEQLQKNLLQLGALAAIALAMGSVSGELDRGTAALV
                     LAQPVTRPAFLAAKLVAIGVVLAIATLLAGLTAFVYTAVLFDIPPIGGWLAMTVLSWL
                     ALTAWAALTFVASAATGSTTAAAGIGFVALIGLSLASIVPVLGRLLPSGLTSPAILLA
                     SGATATVDGGQLLTALLGTVAIIGACLVGAVLAFRRREL"
     gene            complement(1742..>2452)
                     /locus_tag="AC162A_05580"
     CDS             complement(1742..>2452)
                     /locus_tag="AC162A_05580"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006037443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5010346.1"
                     /translation="PRAYGWMTGRDQLRMLGRLHGMGGPSLERAVEAALERVDMTSAA
                     DRRSGAYSGGMRQRLGIAGALVHRPPVAILDEPVSSLDPEGRRDVLNLIAALRGEITV
                     LFSTHVLADVERICDRVGILAHGRLVVEGPLADLLDRYALPVYRVEAEPGQGAALEAL
                     ADRLRAADWVTGVGIEHGLLTVAVADPARAAHEILPAVAAAGIGVISVARARPTLEDV
                     FLRLTDDSTDTAEPGSAA"
BASE COUNT          360 a          886 c          910 g          296 t
ORIGIN      
        1 cgccgtatcc gttgaggacc cgctgcgccg cccggacgaa ctcgtcgagc ttgccgatcg
       61 cgtcgtcgtc gtggtcgtag tcgatgcccc cgaaccgcag gaacaccggg atcgcgggcc
      121 gcagctccgc gaggaactcg ccccggcccg tccggagtcg ttcatagacc gcgggcagca
      181 gccacgggcg gacgaggtcc tcgggcaggg gctccggttg gtggcccggc tcaggctcga
      241 cgtcgaccag gaggccgccg aagacgccac aggcaccgcc cgcgccttcg tcgcgccgca
      301 gctcggcggt ggccacccgg tcgcccaacg ccgcgagggc cgatgcatcg agcacgatct
      361 cgccccgctg ggcgtggtgc tccgcttcgg cgaggtcgtc catcagccgg ccggcgagca
      421 cgtcgatgag ctggatgtcg ggatcaccga cgacgaaccg gcgggcgtcg ccgaccgcga
      481 tcgccacctt catcccgagc tcgaccgtgc tcccgccggg cgtcacgatc gtgcccgtgc
      541 gcccgatcgc ctgctgcatg ccctcggccg ccgcgcaggc gcgggtgccg tcgtcgccgt
      601 cgatccaaca cgtgatggcg tcgcccgcga agtagatgac gttgccgtca taggcatcga
      661 gctcgctgat gacggcatgg aacacgcgcc cgatgttggc ggtgagctcc tcggcgccac
      721 gctggggacc gagctccttg gccaacgcct cggtcagcgg cgtgaagccg ctgatgtcgg
      781 cgaacagcgc cgacccgtgg acgcggtccg ggagcgtgtg tccggcgtgg agcgcccagc
      841 gacggtcgcg cgggatgtag gcgtcaggcc ggtcgctggt gacctcgtcc cgcgacgacg
      901 ttggggaccc cgcgggagga agggtgctgc tcatggcaac ccattgtgcg gggaccgcgc
      961 gacgtgggtg ctcagagctc gcgacggcgg aaggccagca ccgcgcccac gaggcaggcg
     1021 ccgatgatcg cgacggtccc gagcagcgcg gtcaggagct gtccgccgtc gacggtcgcg
     1081 gtcgcacccg acgcgagcag gatcgccggc gaggtcaggc cgctgggcag caggcggccg
     1141 agcacgggca cgatcgaggc gagtgagagc ccgatcaggg ccacgaaccc gatcccggcg
     1201 gcggcggtcg tcgaccccgt cgccgccgac gcgacgaagg tcagcgccgc ccaggcggtc
     1261 agggcgagcc acgacagcac cgtcatcgcc agccagccgc cgatcggtgg gatgtcgaac
     1321 aggacggcgg tatatacgaa cgccgtcagg cccgcgagca gcgtcgcgat cgcgagcacg
     1381 acgccgatcg ccaccagctt cgcggccagg aacgcgggac gggtcaccgg ctgggcgagg
     1441 acgagggccg ccgtgccccg gtcgagctcg cccgacacgc tgcccatcgc cagcgcgatc
     1501 gccgccagcg cgccgagctg gaggaggttc ttctggagct gctcgacggc catcgccgcg
     1561 gtcggcgccg gcagcgcgac cggcagctgg tcgcccagcg ccgcctgcat gatctcgggc
     1621 aggtagcgag cggtcagcgg ggagatcatc ccgatcaccg cgaacagacc ggcgacgatc
     1681 ggcagccggc gcgtctgcca cgcctcggcg agctccttgc ggagcaggac ccggaagccg
     1741 ctcatgccgc gctccccggc tcggctgtat cggtcgaatc gtcggtcagg cgcaggaaca
     1801 cgtcttcgag cgttggtcgc gcgcgggcga ccgagatcac gccgatcccc gcggcggcca
     1861 cggcgggcag gatctcgtgc gcggcgcggg cgggatccgc gaccgcgacg gtgagcaggc
     1921 cgtgctcgat tccgacgccg gtcacccaat cggcggcgcg cagccggtcg gcgagcgcct
     1981 cgagcgcggc gccctgaccc ggctcggcct ccacgcggta cacgggcagc gcgtaccggt
     2041 cgaggagatc cgccagcggc ccctcgacga cgaggcgccc gtgggccagg atcccgaccc
     2101 gatcgcagat ccgctcgacg tccgcgagga cgtgcgtgga gaacaggacc gtgatctcgc
     2161 cgcgcagcgc cgcgatcagg ttgaggacgt cgcgccggcc ctccgggtcg agcgagctga
     2221 ccggctcgtc gaggatcgcc acgggcgggc gatggacgag ggcgccggcg attccgagcc
     2281 gctggcgcat accgcccgag taggcgccgg aacgacgatc cgcggccgac gtcatgtcga
     2341 ccctctcgag ggcggcctcg acggcgcgct cgagcgaggg cccgcccatg ccgtgcagcc
     2401 ggccgagcat gcgcagctgg tcgcggccgg tcatccatcc gtaggcacgc gg
//
LOCUS       DBNHXO010000267         2785 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_866092, whole genome shotgun sequence.
ACCESSION   DBNHXO010000267 DBNHXO010000000
VERSION     DBNHXO010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_866092"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="AC162A_05585"
     CDS             <1..380
                     /locus_tag="AC162A_05585"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR5010347.1"
                     /translation="RELASRALGLVEGLLAGEPVALDDVPIDLGDRSAWDRLVLDGVR
                     SIPRGEVASYGEVARRIGRAGAARAVGGAVARNPVGLFVPCHRVIAGDGSLGGYGVAA
                     WGGREAALDVKRGLLALEGVDIR"
     gene            492..767
                     /locus_tag="AC162A_05590"
     CDS             492..767
                     /locus_tag="AC162A_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010348.1"
                     /translation="MSKNSDLPGTGGDIAMRVDGTEDDVEGHRKRDASEDARRDDSDS
                     ARRDGSESARRVDGGDDDVEGHLYRLGPTTEGEFARRGPGENPNGDR"
     gene            complement(826..1641)
                     /locus_tag="AC162A_05595"
     CDS             complement(826..1641)
                     /locus_tag="AC162A_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5010349.1"
                     /translation="MTTELPAWSEPFVGERRDGLARHGPWGRVDRDWALGDAGGRGIR
                     VAIVDSGVEGSHPAIGGRLVDSVAVERDGADWRVVPAPPDDLVGHGTASAGVIHDLAP
                     EADLVSIRVLGVDNKGNGGGFAAGLAWAIEQSGASIVNLSLSSRSDTFFGQLHELADE
                     AYFRNVLLVSAVNNVAVASYPSLFAAVVSVAAHDVPRPDAWFYNPEPPVEFGAHGLNV
                     DVAWRGGTRMTVTGNSFAAPHITGLAARIRSAHPGITPFETKALLAATADNAT"
     gene            complement(1638..2417)
                     /locus_tag="AC162A_05600"
     CDS             complement(1638..2417)
                     /locus_tag="AC162A_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5010350.1"
                     /translation="MNEPTDDRLLRALEAVAARAAVAARLEPAGGEAVLRSVADATVT
                     LFDAEAASIALYEPATDRLVIRVAAGTAGEGAVGLALAPSEGIAGYVFTTGQPLVVSD
                     VAADPRFDRSTAEGTGYVPRSILAVPLVDDGGTIGVLEVLDRHGDAGFGLRDIELAGV
                     FARQATVAIRASRVERDTGELLRTVLAALAAPDANPDDVEAAVAAAIVGLDDGDDGLW
                     ALADAVARARAAAPGEVALVVDILDALARRAQPPAPRTFRR"
     gene            2510..>2785
                     /locus_tag="AC162A_05605"
     CDS             2510..>2785
                     /locus_tag="AC162A_05605"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5010351.1"
                     /translation="MTAVAQAGAKISPTAVFKKRDFVLMWVAQLVSTAGSSLTDLAAG
                     IYIYSVTGSVFLVGVTLMVTAIPSLIVGLLAGVFVDRYDRRSVMIWTC"
BASE COUNT          383 a         1004 c         1045 g          353 t
ORIGIN      
        1 cgcgcgaact cgcatcgcgt gccctcggcc tggtcgaggg cctcctggcc ggcgagccag
       61 tcgcgctgga cgacgtgccc atcgacctgg gagaccgttc ggcatgggac cggctggtgc
      121 tcgacggcgt gcgctcgatc ccccggggag aggtcgcgag ctacggcgag gtcgcgaggc
      181 ggatcggacg tgccggcgcg gcgcgggccg tgggtggcgc ggtcgcgcgg aaccccgtcg
      241 ggctgttcgt gccctgccac cgggtgatcg cgggcgacgg atccctcggc ggctacggtg
      301 tggccgcgtg gggcgggcgc gaggcggcgc ttgacgtcaa gcgcgggctg ctcgccctcg
      361 aaggcgtcga tatccgctga cggtgtcggc ttgccgacag gctggctgtg aactgggcgg
      421 tcccgtggag ctcgagacac gtcgtagagt cggggcaacg acatcgagat ccagagcgga
      481 gggagcccga cgtgagcaag aacagcgatc ttcccggcac cggcggcgac atcgcgatgc
      541 gtgtcgatgg caccgaggac gacgtcgagg gacaccgcaa gcgggacgcc tccgaggacg
      601 cgcggcgcga cgacagcgac agcgcgcgac gcgacggcag cgagagcgcg cgccgggtcg
      661 acggcggcga cgacgacgtc gagggccacc tgtaccggct cggcccgacg accgagggcg
      721 agttcgcgcg gcgcgggccg ggcgagaacc cgaacgggga ccgctgaccc gctatcgccc
      781 agccacaagg cgacggccgg cctacccagg gccggccgtt tcgattcagg tcgcgttgtc
      841 cgccgtggcc gcgaggagcg ccttcgtctc gaacggcgtg atgcctggat gggccgagcg
      901 gatccgcgcc gcgagccccg tgatgtgcgg cgccgcgaag ctgttgccgg tgacggtcat
      961 tcgggtaccc ccgcgccagg ccacgtcgac gttcagccca tgagcgccga actcgaccgg
     1021 cggctccggg ttgtagaacc aggcgtccgg gcggggcacg tcgtgggccg cgaccgacac
     1081 gaccgcggcg aacagcgacg ggtagctcgc cacggcgacg ttgttcacgg ccgacacgag
     1141 tagcacgttc cggaagtagg cctcgtcggc gagctcgtgg agttggccga agaacgtgtc
     1201 gctgcgagac gagagcgaca ggttgacgat cgacgcgccg gactgctcga tcgcccaagc
     1261 caggccggcc gcgaacccgc ccccgttgcc cttgttgtcg accccgagga cccggatcga
     1321 gaccaggtcg gcctcgggcg cgaggtcgtg gatgacgccc gcgctcgccg tcccgtggcc
     1381 gacgaggtcg tcgggcgggg cggggacgac gcgccagtcc gctccgtcgc gctcgacggc
     1441 gacgctgtcc acgagccgtc ctccgatcgc cgggtgcgag ccctcgacgc cggagtccac
     1501 gatcgcgaca cggatcccgc ggccgccggc atcgcccagc gcccagtcgc ggtcgacgcg
     1561 gccccacggg ccgtgccgtg ccagcccgtc ccgtcgctcg ccgacgaaag gctcgctcca
     1621 cgccggcagc tcggtcgtca ccgtcggaac gtccgcggcg ccggcggctg cgcccggcga
     1681 gcgagcgcgt cgaggatatc gacgaccagg gcaacctccc ccggggcggc ggcgcgagct
     1741 cgggcgacgg cgtccgcgag cgcccacagg ccgtcgtcgc cgtcgtccag gccgacgatt
     1801 gcggccgcga ccgccgcctc gacatcgtcc gggttcgcgt cgggcgcggc aagtgcggcc
     1861 agcaccgtgc gcagcagctc gcccgtgtcc cgttcgaccc ggctggcgcg tatggcgacg
     1921 gtcgcctggc gcgcgaagac cccggcgagc tcgatgtcgc gcaggccgaa gccggcgtcg
     1981 ccgtgccggt cgaggacctc gagaacgccg atcgtgcccc cgtcgtcgac gagcggcacg
     2041 gcgaggatcg accgcggcac gtagcccgtg ccctcggccg tcgaccggtc gaagcggggg
     2101 tcggccgcga cgtcgctgac caccaggggc tggccggtcg tgaacacgta acccgcgatg
     2161 ccctcgctgg gcgcgagcgc gagccccacg gcgccctccc ccgccgtccc cgcggcgacg
     2221 cggatcacga gccggtcggt cgccggttcg taaagggcga tcgacgcggc ctcggcgtcg
     2281 aacagcgtca ccgtcgcatc cgcgacggac cgcaggaccg cctcgccccc cgctggctcg
     2341 aggcgggcgg ccacggcggc gcgcgcggcg acggcctcca gggcccggag caggcggtcg
     2401 tcggttggct cgttcatcag cccaaatcgt agcgagccgg gcacgtacac tttcggccag
     2461 tcctcgccgg ccccgcgacg gacgcccgcc agcgcgaggg agacacagcg tgaccgccgt
     2521 cgcacaggct ggagcgaaga tctcgccgac ggcggtgttc aagaagcgcg acttcgtgct
     2581 gatgtgggtc gcgcagctgg tgtcgaccgc cggctcgtcg ctcaccgacc tcgcggcggg
     2641 catctacatc tactcggtca cggggtcggt cttcctcgtc ggcgtcacgc tgatggtcac
     2701 ggcgatcccg agcctcatcg tgggcctcct ggccggcgtg ttcgtcgacc gctacgaccg
     2761 gcgctcggtg atgatctgga cctgc
//
LOCUS       DBNHXO010000268         1736 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_667900, whole genome shotgun sequence.
ACCESSION   DBNHXO010000268 DBNHXO010000000
VERSION     DBNHXO010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_667900"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="AC162A_05610"
     CDS             complement(<1..435)
                     /locus_tag="AC162A_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010352.1"
                     /translation="MAIADEVLIQPVEAAAPSRPRIWLRLAAGFVLGLVLVGGAAAAG
                     LYAWDQSYEARVLPGVTAAGVNLAGMDRAQATAALDQAYQSVTSGTVVLLTPDGDLSV
                     PYAQFGRRVDTAAMADAALRAGRDGTIAERALGQLRLALTGTI"
     gene            complement(688..>1736)
                     /locus_tag="AC162A_05615"
     CDS             complement(688..>1736)
                     /locus_tag="AC162A_05615"
                     /inference="COORDINATES: protein motif:HMM:NF037016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5010353.1"
                     /translation="APTATVAGSPYPITPSAAVGTGLGNYTIAYVAGSLTVTVAPLTI
                     TADDQSKPSGATFVFAGTEFSTTGLLNGDTVDSATLASAGAPAGAAPGTYPITVSAAV
                     GTGLANYAVTYVPGTFTVGNTVPTVGDASVTTDATVLVAGAVVVTDPDTGQTVTVTIA
                     AAPANGTATVAQDGSFTYQPTGTWTGQDSFTIQGCDDNAIPACSTGTVTVAVYPVAVP
                     DAGVTSEGETVEVDVQANDIGDAGAPQIITPPANGTATIGSIIYTPDTGFAGTDQVVY
                     RICSPNDQTLCDDTTLTITVSSDAPPTDADQAIATPLGPVGSGMLQLAGMLALLLVSA
                     SCAAAFLWRRRPTG"
BASE COUNT          227 a          596 c          658 g          255 t
ORIGIN      
        1 gatcgtgccg gtcagtgcga ggcggagctg gcccagcgct cgttcggcaa tggttccgtc
       61 ccgcccggcg cgcagggccg cgtcagccat cgcggccgtg tcgacgcgcc gtccgaactg
      121 ggcatacggc accgacaggt cgccgtcggg cgtcagcagc acgaccgtgc cggacgtcac
      181 cgattggtac gcctggtcga gcgccgcggt cgcctgggcg cggtccatgc ccgcgaggtt
      241 gacgcccgcg gcggtgacgc ccggcaggac gcgagcctcg tagctctggt cccaggcata
      301 caggccggcg gccgcagcgc cgccaacgag caccagcccg agcacgaatc ctgcggcgag
      361 gcgcagccag atccgcggtc tggacggcgc ggccgcctcc accggctgaa tcaggacctc
      421 gtcagcaatc gccatggcgc cacgcatgat gccgcatcag ttgccgtccg acgacaggta
      481 atttgggggg tattgggtgg tacggggtgg tacgccgcgg tcgggtgcag tcgggacgcg
      541 cggcacgtcg gttcgagcct gcctggaaga cgcgacgctc tcccgtcctg ttgcgagcgc
      601 tggtcctggc gtggcggcac atgcgcgccc tgccgaacct gccgtcgtgg cagcggacgg
      661 cgccctagcc gccccgccgc gccggcgtca gccggtcggg cgacgccgcc acaggaacgc
      721 cgcggcacag gacgcggaca cgagcaacag ggcgagcatg ccggccagct ggagcatccc
      781 ggacccgact gggccaagcg gcgtcgcgat ggcctggtcg gcgtccgtcg gcggcgcgtc
      841 gctcgagacc gtgatcgtca gggtcgtgtc gtcgcacagc gtctggtcgt tcgggctgca
      901 gatccggtac acgacctggt ccgtcccggc gaagccggtg tcgggcgtgt agatgatcga
      961 gccgatggtc gctgtcccgt ttgcgggcgg cgtgatgatc tggggcgccc cggcatcgcc
     1021 gatgtcgttc gcctggacgt cgacctcgac ggtctcgccc tcgctcgtca cccccgcgtc
     1081 gggcacggcg accgggtaga ccgccaccgt gacggtgccc gtcgagcacg caggaatggc
     1141 gttgtcgtcg cagccctgga tcgtgaagct gtcctgaccc gtccacgtgc cggttggctg
     1201 gtaggtgaac gacccgtcct gggcgacggt cgccgtcccg ttcgccggag ccgcggcgat
     1261 cgtcacggtc acggtctggc cggtgtcggg gtcggtgacg accaccgcgc ccgcgacgag
     1321 gaccgtcgcg tccgtcgtga cactggcgtc gccgacggtg ggcacggtgt tgcccacggt
     1381 gaacgtgccg ggcacgtagg tgacggcgta gtttgcaagg cccgtgccga cggcggccga
     1441 gaccgtgatc ggataggtgc ccggggccgc gccggccgga gcacccgcgc tcgccagggt
     1501 cgccgagtca accgtgtccc cgttcagcag gccggtcgtc gagaactcgg tccccgcgaa
     1561 cacgaaggtg gcgcccgagg gcttcgactg gtcgtcggcc gtgatcgtca gcggggccac
     1621 cgtgacggtg aggctgccgg cgacgtaggc gatggtgtag ttgccgaggc ccgtgccgac
     1681 cgccgcggac ggcgtgatcg ggtacgggct gccggcgacg gtcgccgtgg gtgcgg
//
LOCUS       DBNHXO010000269         5242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1660553, whole genome shotgun sequence.
ACCESSION   DBNHXO010000269 DBNHXO010000000
VERSION     DBNHXO010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1660553"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="AC162A_05620"
     CDS             complement(<1..357)
                     /locus_tag="AC162A_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR5010354.1"
                     /translation="MPTTVVAELAAEAAAAWCSRALMEHNHRAYLWAAARGADMGIPF
                     DEELLYVAAMLHDVGLVAAFDNATVSFDQAGGNVAWMFAAGAGWPVERRVRVSVIIVR
                     HMWDSVDPAFDAEGHLL"
     gene            complement(390..1100)
                     /locus_tag="AC162A_05625"
     CDS             complement(390..1100)
                     /locus_tag="AC162A_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HIR5010355.1"
                     /translation="MAGRTPEPLPQVVAVHLEVVDQPGTMTAIAARVAESGGHVRTLS
                     TVRRTPSAGLEPDDKPLAELELEVEIDDAREHALIAGLEGLDAVVAIRLTRALAKVFG
                     KRVIVVGGGAQVAQVALGAVSEADRHNLRGERISVDTIPLVGEEAIASAVRAVADLPR
                     ARLLVLAGSIMGGEISRAAEELRAVGIPIIALNMVGSITEHVDLVVSDPVQAGTMAVM
                     AVADTATFDLARVRGRRY"
     gene            complement(1109..1378)
                     /locus_tag="AC162A_05630"
     CDS             complement(1109..1378)
                     /locus_tag="AC162A_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013962.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR5010356.1"
                     /translation="MTIWFTVQLDDRPGSLARVAQALADRGVNITGIVGVAEDTDGAL
                     MLTTSDAAATREAFTAIGITFEEHDATGGSPADFSISDVVAGRSG"
     gene            1433..1894
                     /locus_tag="AC162A_05635"
     CDS             1433..1894
                     /locus_tag="AC162A_05635"
                     /inference="COORDINATES: protein motif:HMM:NF016001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR5010357.1"
                     /translation="MAKRRGDAYYRFFGTLGNSRVVTRLHPVVYRISGGRGLIGHSLG
                     MLNVVLITTGRQSGQVREAPLLATEDGDRIVIVASKNGSDREPGWVWNLRADPEAKVR
                     VGSSVRDVRAYEASGDERERLWARAVDAYRGYDLYQRKTARRIPVIVLEPR"
     gene            1894..2286
                     /locus_tag="AC162A_05640"
     CDS             1894..2286
                     /locus_tag="AC162A_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HIR5010358.1"
                     /translation="MPAPLGKTPTKKMPNIQVFGLDDSNATRGALRFFRERRIVVHYV
                     DLRKKPIAAGELRRFVDRLGAAALLDTESRAYRDGGLAYLTTDNAGLTRRLLADVHLI
                     RLPLVRYGDDVTAGKAEDTWKAWLAKPR"
     gene            complement(2520..4010)
                     /locus_tag="AC162A_05645"
     CDS             complement(2520..4010)
                     /locus_tag="AC162A_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012308.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HIR5010359.1"
                     /translation="MPEGTELEVIGGPVEASGYTWVHVAPVGVTLDDGATDGWVAIAD
                     HDGTPWVEASAAPLAGLAVAQSSVEKAPVSVADAKRTAAGVNAFGVDLYRRLLKDPGA
                     ELDGKGVSMSPMSIAAALAMARAGAEGATASQMDDVLRTNSWRDLGSGVGSLQQILNG
                     YNATWTDDERATHSLSLNVVNRAFGQDGGAIQEPFLDRIARVFGAGLGLVDFANDTDA
                     ARDTINAWVARQTADRIKKLLGPSDVSANTRLVLVNAIYLKANWLNEFDEDLTAKRTF
                     TMPDGDAVKVPTMRMAGGQNIALAEGPGWQATELKYVGADGSTPLAMTLILPDDLASF
                     EDSLSAGRLNTIQDEIATEQKRIAKVTNPDDGDMNCPRFAYDVELFLPKFGVDTRAGL
                     VPILRAMGMRNATDSQRADFSGITGARDLFIAMVIHQANIDVDEKGTEAAAATAVGMD
                     TTGGCGPPAAFKHKVLRLDRPFMYLIRDTKTGVILFMGRVLDPTKR"
     gene            4444..>5242
                     /locus_tag="AC162A_05650"
     CDS             4444..>5242
                     /locus_tag="AC162A_05650"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR5010360.1"
                     /translation="MTDREQPAKDASSQSPRTIRRGRVSMRPEAAERLSKGRVTDDEK
                     LLAGHAAERPAFLETDTWRALRILSEFVEGFEALAEVGPAVAVFGSARVGERDPAYAL
                     SRRIGGALAEAGFAVITGGGPGVMEAANRGAHEAGGMSIGCNIELPHEQHLNQYVDVS
                     IEFHYFFARKTMFVKYSDAFVIMPGGFGTLDELFEALTLIQTGKIRHFPVVLMGHAYW
                     DGLLAWMREVQLPAGAISQADLDLLRVTDDPDEAVAIITAFASANGIT"
BASE COUNT          771 a         1823 c         1869 g          779 t
ORIGIN      
        1 gagcaggtgt ccctccgcat cgaaggccgg gtcgacgctg tcccacatgt gccggacgat
       61 gatcacggag acccggaccc ggcgctcgac cggccagccg gcgccggcgg caaacatcca
      121 ggcgacattg ccgccggcct ggtcgaacga gacggtggcg ttgtcgaacg ccgcgaccag
      181 cccgacgtcg tggagcatgg cggcgacgta gaggagctcc tcgtcgaacg ggatccccat
      241 gtcggcaccg cgcgccgccg cccagaggta cgcccggtgg ttgtgctcca tcagtgccct
      301 cgagcaccag gcggcagcgg cctccgcggc cagctccgcg acgacggtcg tgggcacgtg
      361 gatatccgca agccgcagca cctgcatcgt cagtacctgc ggccgcgcac gcgggcgagg
      421 tcgaaggtcg cggtgtcggc gacggccatg accgccatcg tgccggcctg gaccgggtcg
      481 ctgacgacga ggtcgacgtg ctcggtgatc gagccgacca tgttgagcgc gatgatcggg
      541 atgccgaccg cgcgcagctc ctcggcggcg cggctgatct cgccgcccat gatcgagccc
      601 gcgaggacca gcaggcgtgc acgcggcagg tcggccacgg cgcggacggc cgatgcgatc
      661 gcctcctcgc cgaccagggg gattgtgtcc accgagatcc gctcgccgcg gaggttgtgg
      721 cgatcggcct ccgacacggc gcccagcgcc acctgggcga cctgcgcccc gccaccgacg
      781 acgatcacgc gcttgccgaa caccttggcc agggcccggg tcagccggat cgcgacgacc
      841 gcgtcgaggc cctcgagccc ggcgatcagc gcgtgctcgc gagcgtcgtc gatctcgacc
      901 tccagctcca gctcggcgag tggcttgtcg tcgggctcga gcccggccga cggtgttcgc
      961 cgcaccgtcg acagcgtccg gacgtgaccg ccggactcgg cgacgcgggc cgcgatggcc
     1021 gtcatcgtcc cgggctggtc gacgacctcg aggtggaccg cgacgacctg cggcagcggc
     1081 tccggcgtgc ggccagccat cgacggcctc agccggaacg gccggcgacg acgtcgctga
     1141 tcgagaagtc cgcgggggag ccgccggtcg cgtcgtgctc ctcgaaggtg atcccgatcg
     1201 ccgtgaacgc ctcgcgggtc gccgccgcat cgctggtcgt gagcatcagc gccccgtcgg
     1261 tgtcttccgc gacgccgacg atcccggtga tgttgacgcc gcgatcggcc agggcctggg
     1321 cgacgcgggc gagtgaaccc gggcggtcgt cgagttgcac ggtgaaccag atcgtcacgc
     1381 ggaacctcct gcgagcccat ggtacgcggg ccgcgcgcta cgctgcgcga cgatggccaa
     1441 gcggcgtggc gacgcgtact accgcttctt cgggaccttg ggcaacagcc gggtcgtcac
     1501 gcgcctgcac ccggtggtct accggatcag cggcggccgc gggctcatcg gccacagcct
     1561 cgggatgctc aacgtcgtcc tcatcaccac cggtcgccag agcgggcagg tccgcgaagc
     1621 gccgctgctg gcgaccgagg acggcgaccg catcgtgatc gtcgcctcga agaacggctc
     1681 cgatcgggag cccggctggg tctggaacct gcgcgcggat ccggaggcga aggtccgggt
     1741 cgggtcgtcc gtccgcgacg tccgtgccta cgaggcctcg ggcgacgaac gcgagcgact
     1801 ttgggcacga gcggtcgatg cgtatcgcgg ctacgacctg tatcagcgca agacggcgcg
     1861 gcgcatcccg gtgatcgtcc tggagccccg ctgatgccgg cgccgctggg caagacgcca
     1921 acgaagaaga tgcccaacat ccaggtgttc gggctcgacg actcgaacgc gacccggggc
     1981 gccctccggt tcttccgtga gcggcgcatc gtcgtccact acgtcgacct gcgcaagaag
     2041 ccgatcgcgg ccggggagct tcggcggttc gtcgacaggc tgggcgccgc ggcactcctc
     2101 gacaccgaga gccgcgcgta ccgcgatgga ggcctggcct acctcactac ggacaacgcc
     2161 ggtctgaccc ggcgcctgct cgcggacgtc cacctgatcc ggctcccgct ggtccgctac
     2221 ggcgacgacg tcacggccgg caaggccgag gacacctgga aggcctggct cgccaagccg
     2281 cgttgaggct gtcggcgacg tgcgctgggc gcacaacgtt gaccaccgcg tgcgccagat
     2341 acacattccc gcccttcgag cgccttggag cggcccgatg tgtgcgtctg aggcacattt
     2401 cgtgcgtcaa cggcccacga cacgctcaag gtgtgccgcg ggcgcacaga tccgacacgc
     2461 cgcgagcccg gcgaagctcc cgcgcgtcag gaatgtgcac cagccgcacg ggcggcgccc
     2521 taccgcttcg tcgggtcgag gacgcggccc atgaacagga tcacgccggt cttcgtgtcg
     2581 cggatcaagt acatgaacgg ccggtcgagg cggagcacct tgtgcttgaa cgccgcgggc
     2641 ggcccgcacc cgcccgtcgt gtccatgccg accgcggtcg ccgcggccgc ctcggtgccc
     2701 ttctcgtcca cgtcgatgtt cgcctggtgg atgaccatgg cgatgaacag gtctcgggcg
     2761 ccggtgatcc ccgagaagtc ggcgcgttgc gaatcggtgg cattgcgcat gcccatcgcc
     2821 ctgaggatcg gcaccaggcc cgcgcgcgtg tccaccccga acttgggcag gaacagctcg
     2881 acgtcatagg cgaagcgggg gcagttcatg tcgccgtcgt cggggttggt gaccttcgcg
     2941 atccgcttct gctcggttgc gatctcgtcc tggatggtgt tcaggcggcc ggccgagagc
     3001 gaatcctcga acgaggcgag gtcgtcgggg aggatcaggg tcatcgcgag cggggtcgaa
     3061 ccatcggcgc cgacgtactt cagctcggtc gcctgccacc cggggccctc cgcgagcgcg
     3121 atgttctggc caccggccat gcgcatcgtg gggaccttga ccgcgtcacc gtcgggcatg
     3181 gtgaaggtcc gcttggccgt caggtcctca tcgaactcgt tcagccagtt ggccttcagg
     3241 tagatcgcgt tgaccaggac gagccgggta ttcgcgctga cgtcgctcgg gcccaggagc
     3301 ttcttgatcc ggtcggccgt ctggcgggca acccacgcgt tgatcgtgtc gcgcgccgcg
     3361 tccgtgtcgt ttgcgaagtc gacgagacca aggcccgccc cgaacacgcg cgcgatccgg
     3421 tcgaggaacg gttcctggat cgcccccccg tcctggccga aggcgcggtt gaccacgttc
     3481 agcgacaggg agtgggtggc gcgctcgtcg tcggtccacg tcgcgttgta gccgttgagg
     3541 atctgctgga gcgatcccac gccgctgccg aggtctctcc agctgttggt gcgcagcacg
     3601 tcgtccattt gcgatgcggt cgcgccttcg gcgcccgccc gggccatcgc gagtgccgcc
     3661 gcaatgctca tcggcgacat ggacacgccc ttgccatcga gctcggcgcc cgggtccttg
     3721 agcagtcgtc gatacaggtc gacgccgaag gcgttgacgc ccgcggcggt ccgctttgca
     3781 tcggcgacgc tcacgggcgc cttctcgacc gacgactggg cgacggcgag acccgccaac
     3841 ggtgcggccg aggcttcgac ccacggcgtg ccgtcgtgat cggcgatcgc gacccaaccg
     3901 tcggtcgccc cgtcatcgag ggtgaccccg accggcgcga cgtggaccca tgtgtagccg
     3961 ctggcctcaa ccgggccccc gatgacctcg agctcagtcc cctcgggcaa gacgggctca
     4021 taacggattg aatcgtcggc gacgcggggc tgtgatcgga cccgcaggcg gtcggagacg
     4081 gtcaccgcga gtgccccggg gacgaagtcg ccctcatcga ccctgggcga aggctcgacc
     4141 tgggcggtgg catcgggcga ggagaccggc tcggtcggtg agggggatga gacggcggat
     4201 gggcttgcgg ccggagacga ggggctcgcg gacggcacga cgctgctgtc gcaggccgcg
     4261 gcgaccagca ggaccgccag tgccgtgacg cgcaatgacc tcgtcgaccg acggttggtg
     4321 ttggtcctcg ggcgcatgcc gccacctcga acgtccgtga tgattccgac gctgagacgc
     4381 gcgacggtgg cacgccgcga cgccgagccg tcggcaccct tcgcggagtg gcatcctgcg
     4441 cgcatgaccg atcgagagca acccgccaag gacgcctcgt cccagtcgcc gcggacgatc
     4501 cggcgggggc gcgtctcgat gcgcccggag gccgccgaac gcttgtccaa gggccgggtg
     4561 accgacgacg agaagctcct cgcgggccat gcggccgagc gccccgcgtt cctcgagacg
     4621 gacacgtggc gggcgctccg gatcctgtcc gaattcgtgg aaggcttcga ggcgctggcg
     4681 gaggtcgggc cggcggtggc ggtgttcggg tcggcgaggg tgggggagcg cgaccccgcg
     4741 tatgcgctgt cacgccggat cggcggcgcg ctcgcggagg ccgggttcgc ggtgatcacc
     4801 ggcggcggcc cgggtgtgat ggaggcggcc aaccgcggcg cccacgaggc cggcgggatg
     4861 tcgatcggct gcaacatcga gctgccccac gaacagcacc tcaaccagta cgtcgacgtg
     4921 tcgatcgagt tccactactt cttcgcccgc aagaccatgt tcgtgaagta ctcggacgcg
     4981 ttcgtgatca tgcccggcgg cttcggcacg ctcgatgagc tgttcgaggc gctgacgctg
     5041 atccagaccg gcaagatccg ccacttcccg gtcgtgctga tgggtcacgc ctactgggac
     5101 gggctgctcg cctggatgcg cgaggtccag ctgcccgcgg gcgcgatcag ccaggcggac
     5161 ctcgacctgc tgcgggtgac cgacgacccc gacgaggcgg tcgcgatcat cacggccttc
     5221 gcgtcggcca acgggatcac cg
//
LOCUS       DBNHXO010000270        10325 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2257294, whole genome shotgun sequence.
ACCESSION   DBNHXO010000270 DBNHXO010000000
VERSION     DBNHXO010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10325)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10325
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2257294"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="AC162A_05655"
     CDS             complement(<1..202)
                     /locus_tag="AC162A_05655"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR5010361.1"
                     /translation="MPRIVAIIIDVIIVGFVYAVVVAVLAAVGGWGAFIIGGLIHLAI
                     SVGYFVYLWTRQRATLGQRLLGI"
     gene            503..1348
                     /locus_tag="AC162A_05660"
     CDS             503..1348
                     /locus_tag="AC162A_05660"
                     /inference="COORDINATES: protein motif:HMM:NF039362.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HIR5010362.1"
                     /translation="MRENRQLLAFPLLSGVALSVATVIFLASLLGTGVVDGMTGTDDQ
                     AKAGSTVLGLVELFVFYLVCYAVVIFSNTALVGAVLRLADGQSASVGAGFRIAFRRLP
                     KIIVYALISATIGLLARSIARSGRDSGNILVAIVAAIVGAVIQGAWSLVVFFAIPVIV
                     VEDLGTIASLKRSLVIFRQTWGEAFTGEAAIGGISCLVYLLIIVGAGALIGLGIAVGM
                     PALIGIAIAVAIVLMCAIAVLSGAVNGVFQASLYRYATTGDAGPLIDTSDAAAAFRGD
                     GASAS"
     gene            1417..1677
                     /locus_tag="AC162A_05665"
     CDS             1417..1677
                     /locus_tag="AC162A_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR5010363.1"
                     /translation="MADVGIRELKQRLSEYLDRAERGEVLRVTDRGRPKAVLGPVPGR
                     ARIEGGIRDGWISPGTGAPLGPVRRAKASRRVLDVLAEDRGE"
     gene            1674..2078
                     /locus_tag="AC162A_05670"
     CDS             1674..2078
                     /locus_tag="AC162A_05670"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR5010364.1"
                     /translation="MSTYVDSSALLKRYIEEPDSAAADALLRADGSLLTARHTIVEVR
                     RNLARLLLGRDLTAARAAFADDLHSISVIELDEATCESAAAIAETTGVRTFDALHLAA
                     AQRIAAPGVGFLTFDLRQAQAARALGLTVVTA"
     gene            2237..2680
                     /locus_tag="AC162A_05675"
     CDS             2237..2680
                     /locus_tag="AC162A_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010365.1"
                     /translation="MSYEEKGQWVYLVAIVLTFGAYVAVVLGQANGGPLTDVDYVPVM
                     LAAIGLGIALAIVGRIVVEIAGGISAEMAGHDDSHDPDVRDRDIGRFGEYFGGTVLGV
                     GMVVPFVLTLAEFDYFWIANAMYLVFFLAAAVGAAVKLVAYRRGM"
     gene            2684..2914
                     /locus_tag="AC162A_05680"
     CDS             2684..2914
                     /locus_tag="AC162A_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020644504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5010366.1"
                     /translation="MGKPTRVSNQIRRLRFETGEMTQADLAQRIGVTRQTIIALEQGR
                     YSPSLEMAFQIAHAFNVTLDDVFRYEPQEGAV"
     gene            2914..3402
                     /locus_tag="AC162A_05685"
     CDS             2914..3402
                     /locus_tag="AC162A_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HIR5010367.1"
                     /translation="MTSPTTTEPGRRARLPPRWVIRAAWTIHRAIYGMTGGRFGLRGQ
                     TAERWGMMRLATTGRRSGKERHAILGYFEDGPNLVTMAMNGWGEPEPAWWLNLQTNPD
                     TTVELGDGPRAVRGRAATPDERPRLWARWAEYDGEEALASWAARRSRETAIVILEPRP
                     PM"
     gene            3588..4916
                     /locus_tag="AC162A_05690"
     CDS             3588..4916
                     /locus_tag="AC162A_05690"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140905 - haloperoxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HIR5010368.1"
                     /translation="MTQPTRPPRARWLSIPMAIVVLTATMLIPAASASTADPTDMVLE
                     WNTNAVNAIGNPNTPPPPPAPQIPPGLGQVPPLAPVNLAIVHAAIYDAVNAIDGTHKP
                     YLLDIHAPSNASQAAAAATAAHHVLVGIVPATLPQVTASLDGLYATSLGKIEDGKAKS
                     DGIIVGAAAAAVMLADRLDDGRNGTRTFDVGTDPGDWRLVPPLSNNVFSWVGEIRPFS
                     LKRPDQIRIAPPPRLKSKQYAREFNEVKALGAQTNSTRTPDQQALANWIVVNPFGPVN
                     QTFRGLATSHDLSTAEQARLFAMTSLSSADAMINCFNNKDFYEFWRPQTAIQEAATDG
                     NRRTTADPAWMSLFPTPGYPDWPSGYNCFAAGMMHAGRAFFGTNNVAFDIVNANATRS
                     YTRFTGYVDDAIEGRILTGFHFRSADELGAFIGKKTAKWVDKHEFKPVHH"
     gene            5422..6099
                     /locus_tag="AC162A_05695"
     CDS             5422..6099
                     /locus_tag="AC162A_05695"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR5010369.1"
                     /translation="MDVRSARRDPWLLVGTLCAFTFIVVAALVMRRGSLPFDDAFAAR
                     VQGLPIPREFWEACSRAGGATVFTVALIFVLVAALTRRLRLAVIVAAILGAQNLFTHV
                     VKDYVARARPPGADLVETSGFSFPSSHALSCTATYGLIAVVVWRSDLPRPLRLIVAVI
                     GVTLPILVGLSRIALDVHYPSDVLAGWLAGVAFVALAATLIAVTDAMTPGPLLPSREV
                     RDEGATG"
     gene            complement(6096..6650)
                     /locus_tag="AC162A_05700"
     CDS             complement(6096..6650)
                     /locus_tag="AC162A_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010370.1"
                     /translation="MTKSARIEAVERATGRSWDDWLRWMASINADQLSHHEIASALIT
                     ELEGMVDNLGWWAQATAVAYEQYVGRRVPGQRPDGTFQTSVSRSMSLGMEALIGAWTD
                     FAAADQDVLDRITGDVRVSGTANRITWRAKGRDAAAITVISEPKKDGTASLVVQVIGT
                     ATLEDNVEAKETWTAITARFAATI"
     gene            6751..7398
                     /locus_tag="AC162A_05705"
     CDS             6751..7398
                     /locus_tag="AC162A_05705"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006378515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR5010371.1"
                     /translation="MSLARVQFFSISLDGFGTGEGLTLEAPFGHAGDRLHEWMFLTRW
                     WHELIGESGGSRGIDDALIRLHDPGVGAEIMGAGKFGPPGWHEDPAWRGWWGSNPPFH
                     TPTFVLTHHPRESIEMEGGTTFHFLDTSPAEALKVAREAAGGRDVRLGGGATMIREFF
                     AAGLVDHMHIVVVPILLGRGVRLWDGMEGLEKDYAIEATSSPSGVTHVTFTRTGL"
     gene            complement(7432..8718)
                     /locus_tag="AC162A_05710"
     CDS             complement(7432..8718)
                     /locus_tag="AC162A_05710"
                     /inference="COORDINATES: protein motif:HMM:NF018479.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HIR5010372.1"
                     /translation="MSQPEPVADPAAVGPEPSAVRHFRRWFWRPPRAHGEIEAERRVS
                     FLELFYDLIYVVVIAQASHQLAGSVSARSYLEFLVIFGVVWVAWANGTLYYELHGRED
                     GRTRTYVFVQMAILALLAVFTGHAAGETGTAFAIVLIAYLVVMTWLWYTVRRQDRPEY
                     MATSARYLAAMLISIVVIAISALLPADARLVVWAAFCIGWLGLMLVFGWRSRRGSVMG
                     IVPTDAMVERFDLLIIIVLGEVVTGVVNGLAGTEHDPLSLATGLVALVVGFGLWWIFF
                     DVGGRRLPRLDGLAINAWMEAHLPIAIAIVGAGAAMVGLIGDAHEAQAPAPVAWLLAG
                     SVALLLVALAGMTRTLADFDRHAVVYRPLALVMLGAAGVALLVGWWRPAPWLLGLLLA
                     AILASVWLFAVVRLFQTGTWTAAGSPDTASEPPAAR"
     gene            complement(8735..9391)
                     /locus_tag="AC162A_05715"
     CDS             complement(8735..9391)
                     /locus_tag="AC162A_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010373.1"
                     /translation="MATGTLAPTPPLLAGGEDLSGTARSAARAAFDRILIEPTPADLW
                     QLQKALLVLDGEPAARARAVARAFHGCLRNLESKSASRRASRWGAVLGTAAVGSVSVG
                     DMADTQENALGRLLQSAVPAMLEVGSAVKSAEAWEVEAGLLYDDVAWFLYDELWDVSA
                     TARPELSPSERRDQIDLLIDPLVDAALPDGDRAALVVNVFRAVLAARLLPVLDEPDGR
                     "
     gene            9472..10032
                     /locus_tag="AC162A_05720"
     CDS             9472..10032
                     /locus_tag="AC162A_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010374.1"
                     /translation="MDKRLGAAGLALGLSLTLAACSGGAGSTAQTGGGTPTEAAGAPE
                     TPEAGGGAGASAAAGGGANGFEGTLASSGLYSANWTVNPETEPNPINASNNPSLVSDK
                     NTFGNIKVETDGSTSFGSAAAELNANGSYDGTGAKVTLDSTGQFVCAFTIDNDLKGSN
                     DGAILHMSGGLTVHWHPEGVGDLSCP"
BASE COUNT         1616 a         3611 c         3510 g         1588 t
ORIGIN      
        1 cgatgcccag cagccgctga ccgagcgtcg cacgctgtcg cgtccacagg tacacgaagt
       61 agccaacgct aatggccaaa tggatgaggc cgccgatgat gaacgcaccc cacccgccga
      121 cggctgcgag gacggcgacc acgacggcat agacgaaccc gacgatgatc acgtcgatga
      181 tgatcgcgac gatccggggc accagtggtg cgtacgcgac gccaggcgca ggcccggctg
      241 ctgcgggcga cggggtatgc ccggcgggat ccgtcatgcg actacctctc acatcggcct
      301 gaagcgcgtc gcgcccggtc aggatagcca attgcccagg tcgtccgtgg gcgaacacgt
      361 tggccggatc gcgcaggccg ctccaggcgg cgtgccggac cggtaacctc tgccctcgtt
      421 ggcccgcaca gtgaggaggc gtctggtcgt ggcagacagc atcaccaacg gagcgcgtct
      481 ctccacgcaa tcgtggagag cgttgcgcga gaaccgccag ctgctggcct tcccgctgct
      541 gtccggcgtg gctctgagcg tcgccacggt gatcttcctg gcatcactcc tgggcaccgg
      601 ggtggtcgac ggcatgaccg gcacggacga ccaggcgaag gccggctcca cggtcctggg
      661 cctggtggaa ctgttcgtct tctatctcgt ctgctacgcc gtcgtgatct tctccaacac
      721 ggcgctggtc ggcgcggtgc tgaggcttgc cgatggccag tcggccagcg tcggcgcagg
      781 gttccgcatc gccttccgac gcctgccgaa gatcatcgtg tacgccctga tctctgcaac
      841 cattggcctc ctggcgcgga gtatcgcgcg gtcgggccgc gactcaggca acatcctcgt
      901 cgccatcgtg gccgcgatcg tcggggcggt catccagggt gcctggtcgc tcgtcgtgtt
      961 cttcgcgatc ccggtcatcg tggtcgagga cctcgggacg atcgcgtcgc tcaaacgcag
     1021 cctggtgatc ttccggcaaa cctgggggga ggcgttcacc ggcgaagccg ccatcggcgg
     1081 gatctcgtgc ctcgtgtacc tcttgatcat cgtcggcgcc ggggcgctca tcggtctcgg
     1141 gatcgcggtt ggcatgccgg cgctgatcgg cattgcgatc gcggtggcga tcgtcctgat
     1201 gtgcgccatt gcagtgctca gcggcgcggt caacggcgtg ttccaggcgt ctctctatcg
     1261 atatgccacc acgggcgatg cagggccgtt gattgacacg tcggacgcag cagcggcgtt
     1321 ccggggtgac ggcgcgtcgg cttcctgacg ggagccttgg cgacggagcg ggcgcttgcg
     1381 ccgcttgtag acacatcgtc gtggtagtgt gtccacatgg ccgatgtcgg gatccgcgaa
     1441 ctgaagcagc gactgtccga gtacctggac agggcagagc gcggcgaggt cctgcgcgtg
     1501 actgaccgtg ggcgaccgaa ggccgtgctc ggcccggtgc ctgggcgcgc gcggatcgag
     1561 ggcggcatcc gggacggttg gatctcgccg gggacgggcg cgccgctcgg tccggtacgg
     1621 cgggcgaagg cgtcccggcg ggtgctcgac gtcctggccg aggaccgcgg tgagtgagca
     1681 cctacgtcga ctcgagcgcc ctgctcaagc ggtacatcga ggagcccgat tccgctgccg
     1741 ctgacgcgtt gttgcgagcc gatggctcgc tcctgaccgc ccggcacacg atcgtcgagg
     1801 ttcgacgcaa cctcgcccgc ctcctcctgg ggcgcgacct caccgccgcg cgggccgcgt
     1861 tcgctgatga ccttcactcc atctctgtca tcgagctcga cgaggcaacg tgcgaatcgg
     1921 ctgcggcgat cgccgaaaca accggcgtgc ggacgttcga cgccctgcac ctggcagccg
     1981 cgcagcggat cgcggcgccg ggggttggct tcctgacctt cgacctgcgc caggcacagg
     2041 ccgcgcgggc gctcggcctc acggtcgtga ccgcctgagc tcgttcgccc caacccgtct
     2101 gtgcgcactt ctgcttgacc gcaccacacc gggctcggcc gaccttgtga gcgtcgtgtg
     2161 taaagcactc ttgacatgat gtcagccaca ccgtacattc gacaatgtca aacgtccttt
     2221 acatcggagc ggatgcatgt cctacgagga gaagggccag tgggtctacc tcgtcgccat
     2281 cgtgctgacc ttcggcgcct acgttgccgt cgttctcggg caggccaatg gaggcccgct
     2341 gactgacgtc gactacgttc cggtgatgct ggcggcgatc ggcttgggca tcgccctcgc
     2401 catcgtcggt cgcatcgtgg tcgagatcgc cggtggcatc tcggccgaga tggcgggtca
     2461 cgatgacagc cacgacccgg acgtccgcga ccgcgacatc gggcgcttcg gcgagtactt
     2521 cggtggcacg gtgctcggtg tcggcatggt cgtgccgttc gtcctgacgc tggcggagtt
     2581 cgactacttc tggatcgcga acgcgatgta cctcgtgttc ttcctggcgg cggccgtcgg
     2641 tgcggccgtg aagctcgtcg cctatcgacg aggcatgtag cggatgggca agcccacgcg
     2701 ggtcagcaac cagatccggc gcttgcgttt cgagaccggc gagatgaccc aggctgacct
     2761 cgcccagcgg atcggtgtca cgcgccagac gatcatcgcc ctcgaacagg gccgctactc
     2821 cccatccctg gagatggcct tccagatcgc tcacgcgttc aacgtcacgc tcgacgacgt
     2881 ctttcggtac gagccacaag aaggagccgt gtgatgacat ctccgactac caccgagccc
     2941 gggcgccggg caaggctccc gccgcgctgg gtcatccgcg ctgcctggac gatccaccgc
     3001 gccatctacg gcatgacggg cggccggttc ggcctccgcg gccagacagc cgagcggtgg
     3061 gggatgatgc gcctggcgac caccggacgc cgctcgggca aggagcggca cgcgatcctc
     3121 ggctacttcg aggacggtcc gaacctggtc accatggcca tgaacggctg gggggaaccg
     3181 gagccggcct ggtggctcaa cctccagacg aaccccgaca ccacggtcga gttgggcgac
     3241 gggccgcggg cggtccgcgg ccgcgcggcc actccggacg agcgaccacg cctctgggcc
     3301 cgctgggccg agtacgacgg cgaggaggcg ctcgcgagct gggcggcccg ccgatcccgc
     3361 gagacggcga tcgtcatcct cgagccgcgg cccccaatgt gacccagccg ggcatcaggc
     3421 ccgcgcacca ggtcgagtcc tcgctcccga cggctcacgc acggcatctc ggaattcgtc
     3481 ctctgaccgg cgaagattga acggttggac cccgcgaacc gtccttacta ggtatcgggc
     3541 ccccgcgggc acgtcagccc gcgcgacgac ccccggaggg acgggcgatg acccagccca
     3601 cgcgaccccc gagagcacgg tggctctcga tccccatggc gatcgtcgtg ctgaccgcga
     3661 cgatgctgat cccggccgca tcggcatcca ccgccgatcc gaccgacatg gtcctcgagt
     3721 ggaacaccaa cgcggtcaac gccatcggaa acccgaacac tcccccaccg ccgccggcgc
     3781 cgcagattcc gcccggcctg ggccaggtgc cgccgctggc accggtcaat ctcgcgatcg
     3841 tccatgccgc gatctacgac gcggtcaacg cgatcgacgg cacgcacaag ccgtatctcc
     3901 tcgacatcca cgccccgtcg aacgcatcgc aggccgccgc cgcggcgacc gcggcgcacc
     3961 acgtgctcgt cggaatcgtc ccggcgaccc tgccccaggt caccgccagc ctcgacgggc
     4021 tctacgcgac ctcactgggc aagatcgagg atggcaaggc gaagtccgac gggatcatcg
     4081 tcggtgccgc ggccgcggcc gtgatgctcg ccgatcggct cgatgacggc aggaatggca
     4141 cgaggacgtt cgacgtcggc accgacccgg gcgactggcg cctcgtgccg ccgctcagca
     4201 acaacgtctt ctcgtgggtt ggcgagatcc gaccgttctc cctgaagcgc ccggatcaga
     4261 tccgcatcgc cccaccgccg cgcctcaaga gcaagcagta cgccagggag ttcaacgagg
     4321 tcaaggcgct cggcgcccag acgaactcga cccggactcc cgatcagcag gcgctggcga
     4381 actggatcgt cgtgaacccg ttcggcccgg tgaaccagac cttccgcggc ctcgcgacga
     4441 gccacgacct ctcgacagcc gagcaggccc gcctgttcgc gatgacgagc ctgtcgtcgg
     4501 ctgacgcgat gatcaactgc ttcaacaaca aggacttcta cgagttctgg cgaccgcaga
     4561 cggccatcca ggaggcggca acggacggca acagaaggac gaccgccgat cccgcctgga
     4621 tgtcgctgtt cccgaccccg ggctaccccg attggccgtc cggttacaac tgctttgccg
     4681 cggggatgat gcacgccggc agggcgttct tcgggaccaa caacgtcgcc ttcgacatcg
     4741 tgaatgcgaa cgccacgcgg agctacaccc ggttcacggg ctacgttgac gacgcgatcg
     4801 aaggtcgcat cctcaccggc ttccacttcc ggagcgccga cgagctcggc gccttcatcg
     4861 gcaagaagac cgccaagtgg gtggacaagc acgagttcaa gcccgtccac cactgacggg
     4921 ctctcgtcac gacccccgac cgctggggcg gccgggggtc gttgtctgct cagcgccgtg
     4981 ccgagcctgg gacacgccgc gtcaggcgag ccgccagcgc acggcaccgt tcctgccgac
     5041 gagcagaagg tatgtcggcg tcgcccccgt cgggaccgcg aagacgaggg ggccgcggac
     5101 acattcgttc gccctgaatg acccgtcgga cgcgtagcct cgtcggtcga gtcggcatcg
     5161 gcctcggggt cgatggtcga gccgtcgccc atctcgagcg cgacttcata gcggatgttg
     5221 cccaacttca cctcccgcgg cgtcgggtag cgaaggcggc gcagaagcgg gcgcgccgct
     5281 cggcgccgcc gagggccctc cacaagcccc cgtgagcagg ccggtcgcga tcgcgaggcg
     5341 gaggcatgct cccagtgaac gactcgccat gcccgcagta tgggcgggga cgcatcggtc
     5401 cggctcgcta cgctgtgcgc aatggatgtg cgctcggcgc gacgcgaccc gtggctgctg
     5461 gtgggcaccc tttgcgcgtt caccttcatc gtcgtcgcgg cgctcgtcat gcgacgcggc
     5521 agccttccct tcgacgacgc gttcgccgct cgggtccagg gactgccgat cccgcgcgag
     5581 ttctgggagg cgtgctcgcg cgctggtgga gcgaccgtct tcaccgtcgc gctcatcttc
     5641 gtcctggttg cggccctcac tcgtcgcctg cgacttgcgg tgatcgtggc cgcgatcctg
     5701 ggcgcacaga acctgttcac gcacgtcgtc aaggactatg tcgcgagggc acgaccaccc
     5761 ggcgcggatc tcgtagaaac gtcagggttc agctttccgt cgagccacgc cctgagctgc
     5821 accgcgacct acggcctcat cgccgtcgtg gtctggagga gcgatttgcc gcgtccgctg
     5881 cggctgatcg tcgccgtcat cggcgtgacg ctcccgatcc tggtcggtct ctcgcggatt
     5941 gccctcgacg tgcactatcc gagtgatgtc ctcgccggct ggctggcggg cgtcgccttc
     6001 gtggcgctgg cagcgacgct catcgccgtc accgacgcga tgaccccagg accccttctg
     6061 ccgtctcggg aagtccgaga cgaaggagcg acgggttaga tcgtggccgc gaaccgcgcg
     6121 gtgatcgccg tccaggtctc cttggcctcg acgttgtcct cgagcgtcgc cgtcccgata
     6181 acctggacga ccagcgacgc ggtcccgtcc ttcttgggct cgctgatgac ggtgatcgcc
     6241 gcggcgtccc gtcccttggc acgccaggtg atgcgattcg ccgtgccgct cacgcggacg
     6301 tcgccggtga tccggtcgag gacgtcctgg tcggccgcgg cgaagtcggt ccaggccccg
     6361 atgagggcct ccatgccgag tgacatcgac cggctgacgc tcgtctggaa cgtgccgtcc
     6421 ggccgctgcc cgggcacccg tcggccgacg tactgctcgt aggcgacggc ggtggcctgg
     6481 gcccaccagc cgaggttgtc gaccatgccc tcaagctctg tgatcagcgc ggacgcgatc
     6541 tcgtgatgcg acagctggtc ggcgttgatg gaggccatcc acctcaacca gtcgtcccag
     6601 ctccggccgg tcgcgcgctc gacggcctcg attcgggcgc tcttcgtcac tcccctagga
     6661 tacggaggcg atccgtgact gcggatgcgg acggatcgat gtggtctctc accgcgagtg
     6721 atggcccggt cgcaggaagg aacgcccgtc atgtcactcg ctcgcgtcca gttcttctcc
     6781 atctcgctcg acgggttcgg gaccggcgag gggctgaccc tcgaggcgcc cttcggccac
     6841 gctggtgacc ggctccacga gtggatgttc ctgacccgtt ggtggcacga gctgatcggc
     6901 gagtccggcg gcagccgtgg aatcgacgat gcgctcattc gcctacacga cccgggggtc
     6961 ggcgccgaga tcatgggtgc cgggaagttc ggtccgcccg gatggcacga ggacccggcc
     7021 tggagggggt ggtggggttc gaacccgccg ttccacacgc cgaccttcgt cctgacccac
     7081 catccccgcg agtcgatcga gatggagggc ggcacgacgt tccacttcct cgacacgtcg
     7141 ccggccgagg ccctgaaggt ggctcgggag gcggccggcg gccgggacgt ccggctcggt
     7201 ggtggcgcga cgatgatccg cgagttcttc gctgccgggc tcgtcgacca catgcacatc
     7261 gtggtggtcc cgatcctgct gggacgaggc gtccgcctgt gggacggcat ggagggcctt
     7321 gagaaggact acgcgatcga ggcaacctcg tcgcccagcg gcgtgaccca cgtgacgttc
     7381 acgcgtaccg gcctctgagt cgctcggtca ggggcgctga ggtaggtcga gtcagcgagc
     7441 cgctggcggc tcgctcgcgg tatccggcga gccggccgcc gtccacgtcc ccgtctggaa
     7501 cagccgcacg accgcgaaca gccagacgga cgcgaggatc gccgccagga gtagcccgag
     7561 gagccagggg gcgggccgcc accagccgac gagcagcgcc acgcctgccg ccccgagcat
     7621 gaccagggca agaggtcgat acacgacggc gtgccggtcg aagtcggcga gcgtgcgtgt
     7681 cataccggcg agggctacca gcagcagcgc gaccgagccg gcgagcagcc aggcaacagg
     7741 cgccggagcc tgcgcctcgt gcgcgtcgcc gatgaggccg accatcgcgg cgcccgcccc
     7801 cacgatcgcg atggcgatag gcaggtgcgc ctccatccag gcgttgatgg ccagcccgtc
     7861 gaggcgcggc agacggcgcc caccgacgtc gaagaagatc caccacagcc cgaagccgac
     7921 caccagcgcg acgaggccgg tcgccaggct gagcggatcg tgctcggtgc cagcgagccc
     7981 gttgaccacg cccgtcacga cctcccccag gacgatgatg atcagcaggt cgaagcgctc
     8041 gaccatcgcg tccgtcggca cgatgcccat caccgatccg cgccgcgacc gccagccgaa
     8101 gaccagcatc agtccgagcc acccgatgca gaacgcggcc cagacgacga gccgtgcgtc
     8161 ggccggcagg agcgcgctga tcgcgatgac gacgatcgag atgagcatcg ccgcgaggta
     8221 tcgggccgag gtcgccatgt actcgggccg gtcctgccgt cggacggtgt accagagcca
     8281 cgtcatcacg accaggtagg cgatgaggac gatcgcgaag gcggtgccgg tctcgccggc
     8341 ggcgtggccg gtgaacacgg cgagcagggc gaggatcgcc atctggacga acacgtacgt
     8401 ccgcgtccgc ccgtcctcgc ggccgtggag ctcgtagtac agcgtcccgt ttgcccaggc
     8461 gacccacacc acgccgaaga tcacgaggaa ctcgagatag ctacgggccg agaccgaacc
     8521 cgccagctgg tgtgaagcct gggcgatgac gacgacgtag atgaggtcgt agaacagctc
     8581 gaggaagctg accctgcgct ccgcctcgat ctcgccgtgc gcccttggcg gccgccagaa
     8641 ccagcgccgg aagtggcgga cagcactcgg ttctgggccg acggccgccg ggtcggccac
     8701 gggctccggc tggctcacgg tgtggtcgcg ccgctcaccg tccgtccggc tcgtcgagga
     8761 cggggagcag gcgcgccgcg agcacggctc ggaagacgtt gaccaccagg gctgcgcggt
     8821 cgccgtcagg cagcgctgcg tcgacgagcg gatcgatgag caggtcgatc tggtcgcggc
     8881 gctcggacgg cgagagctcg ggccgcgcgg tggcggagac gtcccacagc tcgtcgtaca
     8941 ggaaccaggc aacgtcgtcg tagagcaggc cggcctcgac ctcccacgcc tcggccgact
     9001 tgacggcgga gccgacctcg agcatcgccg gcaccgcgct ctgcagcagg cggccgagcg
     9061 cgttctcctg cgtgtcggcc atgtcgccga cgctgacgct gccgacggcc gcggtgccga
     9121 gcacggcccc ccagcggctg gccctgcgcg acgccgattt gctctcgagg tttcggaggc
     9181 agccgtggaa cgcccgtgcg accgcgcgcg ctcgcgccgc gggctcgccg tccaagacga
     9241 ggagcgcctt ctggagctgc cacaggtccg cgggcgtggg ttcgatcagg attcgatcga
     9301 acgccgcccg tgccgcggag cgcgccgttc cgctcaggtc ttcgcctcca gccaggagtg
     9361 ggggtgtcgg tgccagcgta cccgtggcca tcacgccgcg gcaggtctat ggttgcgccg
     9421 tccgagcggg aggtgacgct tgttgaccgg acgctcggca ggaggcgcga catggacaag
     9481 cgtcttgggg ccgccgggct cgccctcggg ctgagcctca cccttgcagc ctgcagcgga
     9541 ggagccggct cgaccgcgca gacgggaggc gggaccccaa ccgaggcggc cggcgccccc
     9601 gagaccccgg aagccggcgg cggagccggc gcgagtgcgg ccgcgggtgg gggtgcaaac
     9661 gggttcgaag gcaccctcgc cagctccggc ctctactcag ccaattggac ggtcaacccc
     9721 gagaccgagc ccaatccgat caacgcgagc aacaacccgt ccctggtctc cgacaagaac
     9781 acgtttggca atatcaaggt cgagacggac ggcagcacct cgttcggcag cgcggccgcg
     9841 gagttgaacg ccaacggttc ctacgacggc accggggcca aggtcacgct ggactcaacg
     9901 gggcagttcg tctgcgcgtt cacgatcgac aacgacctga agggcagcaa cgacggcgcc
     9961 atcctccaca tgtcgggcgg actgacggtc cactggcacc cggagggcgt cggcgacctc
    10021 agctgcccct gacctcggcg atcgtcaggg cgtcggtcgc agatcgggag ccttgcgcac
    10081 acgtcggtcg ccagcgacgc gaaacgcgtc gtggtcgccg ccactcgcgc gaagaacgag
    10141 gtctcgttgc atcggcgtcc cagtctgtcg cagccacggt ggccactgcc gacactatga
    10201 gccgagaacc cgcggcatcg ttccatcttc gccgttgacc gcgggggccc ctcgctcgtg
    10261 gtcgaggccc gtcagactcg catcgtgtcc agcccgctgc tcgcgaccaa gctctacgcc
    10321 cccgg
//
LOCUS       DBNHXO010000271         2975 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3845020, whole genome shotgun sequence.
ACCESSION   DBNHXO010000271 DBNHXO010000000
VERSION     DBNHXO010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3845020"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="AC162A_05725"
     CDS             <1..705
                     /locus_tag="AC162A_05725"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIR5010375.1"
                     /translation="ERHLVVVAAGDVPAREALDVAWPGWDTAIAGVVAADGGLAGAEG
                     LGLTVDVVVGDLDSADPIRLTAAQAAGTRVAAASSDKDESDTELAVLEALRLGATRVT
                     ILGALGGSRLDHALANVWLLGHEALADTPAVLLDDEVRVSLLFAPLPGGVAIERLLPG
                     RIGATVSLLPFNGPVDGVTTRDLRYPLRGEPLEAGPARGLSNVRTGPAASVAIVGGSL
                     LIIEAAMPPGGLSSEP"
     gene            702..1187
                     /locus_tag="AC162A_05730"
     CDS             702..1187
                     /locus_tag="AC162A_05730"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR5010376.1"
                     /translation="MSTPQSGDPAPPVALPDEQGTIHRLTDRTGSWTVVYFYPEDDTP
                     GCTTEACQFRDLHAEIGGLDAEIWGISPDGSGSHAAFRAKFGLPFTLLSDEDHAVAEA
                     YGAWQEKNNYGRKYWGIVRSSFLVDPDGKVAVAWPRVKADGHAAQVLAALESARAERA
                     G"
     gene            1350..2108
                     /locus_tag="AC162A_05735"
     CDS             1350..2108
                     /locus_tag="AC162A_05735"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR5010377.1"
                     /translation="MADEARKAPHTWRPARFMVIVAHPDDADFGPAATAARWIDEGSE
                     GWLVCCTSGDAGADDWQTDPLELAALREAEQQAAAAIVGYAGVSFLHQPDGALANDLA
                     LREQLVREIRTFRPDAVLAVDPEVLFYDEDGVNHVDHRAAGIAAVDAVYPAARNAMAF
                     PWLARDGLAPHTVRRLYLFWSGRPSAWVDVSATLDRKIDALRAHASQIRHPEQLESRI
                     REWAKEEGAAIGVEASEALRVIVIEADEEPGAEG"
     gene            complement(2112..>2975)
                     /locus_tag="AC162A_05740"
     CDS             complement(2112..>2975)
                     /locus_tag="AC162A_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010378.1"
                     /translation="EAGSAELRAALPRLERMGVEADAARISAEMAESTCHEARVQLAT
                     CEEAETVARMAAASPAAAAPVAPAPIADIGLDDAPIGEAPADDWDISPGSFERQMPAA
                     AIGAYPVIVRILRGDREARERFVATVAAGDPEAERAWRARTDQLVGAITARAIEDGYL
                     DMPDDDTFWSLFAPGESREIVAALSALGYRYDGAGGFADERVPAQRDLSLAVGYAGLD
                     RMRIRNWPREQELSELYQNATVASDEWLVHHAGDLTLGEMVDALGLRAGNLAEVWNAW
                     GRVRPVLLAQD"
BASE COUNT          396 a         1091 c         1091 g          397 t
ORIGIN      
        1 gagcggcatc ttgtcgtggt cgccgcgggc gacgttccag cacgcgaggc gctggacgtg
       61 gcctggccgg gctgggacac ggcgatcgcc ggggtcgtcg cagcggacgg gggactcgcg
      121 ggggccgaag ggctcggcct caccgtcgat gtggtcgtcg gcgacctcga ctcggccgac
      181 cccatacgcc tgacggcggc gcaggcggcg ggcacgcggg tcgccgcggc gtccagcgac
      241 aaggacgagt cggatacgga gctcgcggtc ctcgaggcgc tgcgcctggg tgcgacgagg
      301 gtgacgatcc tgggtgcgct cggtggctcg cgcctcgacc acgcgctcgc caacgtctgg
      361 ctcctgggtc acgaagcgct ggctgacacg ccggccgtgc tgctcgacga cgaggtgcgg
      421 gtgtcgctgc tcttcgctcc gctccccggg ggagtcgcga tcgagcgcct cttgcccggc
      481 cggatcggcg cgacggtgtc gctgctgccg ttcaacggtc cggttgacgg agtcacgacg
      541 cgggacctcc gctacccgct gcggggggag ccgctcgagg caggaccagc caggggactg
      601 tcgaacgtgc gcaccgggcc cgcggcctcg gtcgcgatcg tcgggggcag cctcctcatc
      661 atcgaggccg cgatgccccc gggagggcta tcctcggagc catgagcacg ccgcagtccg
      721 gcgacccggc acccccggtc gccctccccg atgagcaggg cacgatccac cggctgaccg
      781 accggaccgg gagctggacc gtcgtctact tctatccgga ggacgacacg cccggctgca
      841 cgaccgaggc ctgccagttc cgggacctcc acgccgagat cggcggcctg gatgccgaga
      901 tctggggcat cagccccgac ggttccggca gccacgcggc cttccgcgcg aagttcgggc
      961 tgccgttcac gctcctctcc gacgaggatc acgccgtcgc cgaggcctac ggcgcgtggc
     1021 aggagaagaa caactacggg cgcaagtact gggggatcgt gcgctccagc ttcctcgtcg
     1081 atccggacgg gaaggtggcc gtggcctggc ccagggtcaa ggccgacggg cacgccgcgc
     1141 aggtcctcgc ggccctcgag tccgcccgcg ccgagcgggc cggttgagcg cgccgccggc
     1201 cgttttcggg ccatacagag aggcttcgcc cgtgggggga gacgagcgaa gcctcaggcg
     1261 cgactctaac acaacgcccc ttcccgattt caaggccaca aggcatcgcg acgagcgatt
     1321 ccgtgtcccc cgggcaacag tcgccggctg tggctgacga ggcccggaag gcgccccaca
     1381 cgtggcgtcc ggcgcggttc atggtcatcg tcgcgcaccc ggacgacgcg gacttcggcc
     1441 ccgccgcgac cgccgcccgc tggatcgacg aaggctccga gggctggctc gtgtgctgca
     1501 cgagcggcga cgccggggcc gacgactggc agacggaccc gctcgagctc gcggcgctgc
     1561 gggaggcgga gcagcaggcc gccgcggcca tcgtcggcta cgccggcgtg tcgttcctgc
     1621 accagccgga cggggcgctg gccaacgacc tcgccctgcg cgagcagctc gtgcgcgaga
     1681 tccggacctt ccggccggac gccgtgctgg ccgtcgaccc cgaggtcctg ttctacgacg
     1741 aggacggcgt caaccacgtc gaccatcgtg ccgccgggat cgcggccgtc gacgcggtct
     1801 atccggccgc ccggaacgcg atggccttcc cgtggcttgc ccgcgacggg cttgctccgc
     1861 acacggtacg caggctgtac ctgttctggt ccggccggcc gtcggcgtgg gtcgacgtca
     1921 gcgccacgct cgatcgcaag atcgatgccc tgcgcgccca cgcgagccag atccggcacc
     1981 ccgagcagct ggagtcccgg atccgtgagt gggcgaagga ggagggagcg gcgatcgggg
     2041 tcgaggcgtc cgaggcgctg cgcgtgatcg tgatcgaggc ggacgaggag cccggcgccg
     2101 agggctgagg ctcagtcctg cgcgagcagc acgggccgca cgcggcccca ggcattccag
     2161 acctcggcca ggttgccggc ccgcaggccg agggcgtcca ccatctcgcc cagcgtcagg
     2221 tcgcccgcgt ggtgcaccag ccactcgtcg gacgcgacgg ttgcgttctg gtacagctcg
     2281 gacagctcct gctcgcgcgg ccagttgcgg atccgcatcc ggtcgagccc ggcgtagccg
     2341 acggcgagcg acaggtcgcg ttgcgccggg acgcgctcgt cagcgaagcc gccggcgccg
     2401 tcgtaccggt agcccagtgc cgacagggcg gcaacgatct cgcgtgactc gcccggggcg
     2461 aacagggacc agaaggtgtc gtcgtccggc atgtcgaggt agccgtcctc gatcgcgcgg
     2521 gcagtgatcg cgccgacgag ctggtcggtc cgcgcccgcc acgcccgctc ggcctccggg
     2581 tcgccggccg ccaccgtcgc gacgaaccgc tccctcgcct cgcggtcccc gcgcaggatg
     2641 cgcacgatga ccggatacgc gccgatcgcc gcggcgggca tctggcgctc gaaggacccg
     2701 ggcgagatgt cccaatcgtc ggccggtgcc tcgccgatcg gcgcgtcatc cagcccgatg
     2761 tcggcgatcg gggcgggcgc cacgggcgct gcggcagccg gtgacgccgc ggccatgcgg
     2821 gctaccgtct cggcctcctc gcacgtcgcg agctgcaccc gcgcctcgtg gcaggtcgac
     2881 tccgccatct cggcgctgat ccgcgcggcg tccgcctcca cgcccatccg ctccagccgg
     2941 ggcagggcgg cccggagctc ggccgatccg gcctc
//
LOCUS       DBNHXO010000272         1607 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3447313, whole genome shotgun sequence.
ACCESSION   DBNHXO010000272 DBNHXO010000000
VERSION     DBNHXO010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3447313"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="AC162A_05745"
     CDS             complement(<1..185)
                     /locus_tag="AC162A_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003189308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase family protein"
                     /protein_id="HIR5010379.1"
                     /translation="MSLYATTSQTVGPYLHIGLTWLVREDLVGPGVTGEPITIQGRVL
                     DGDGKPVNDALVEIWQA"
     gene            complement(196..912)
                     /gene="pcaH"
                     /locus_tag="AC162A_05750"
     CDS             complement(196..912)
                     /gene="pcaH"
                     /locus_tag="AC162A_05750"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HIR5010380.1"
                     /translation="MSETTGYRRPYVGTQPDYLFPKYASTVKRAPTKPLVVLPQTLSE
                     LTGPLFGHDEVKPGDADLTKQHKGEPIGERIIVSGRVLDENGRAVPHTLVEVWQANSS
                     GRYPHRVDQHNAPVDPNFSGAGRTLTDAEGRYRFTTIRPGEYPWRNHYNAWRPAHIHF
                     SLFGPAFATRIVTQMYFPGDPLLDYDPMYLCVPDEKARKRLVSLFDWETTVPEFALGY
                     KFDIVLRGRDETPQDPSPGE"
     gene            complement(978..>1607)
                     /locus_tag="AC162A_05755"
     CDS             complement(978..>1607)
                     /locus_tag="AC162A_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HIR5010381.1"
                     /translation="RDQMAARVARDIPEGAYVNLGIGLPTRVANFLPKDREIFLQSEN
                     GLLGMGPAPAKDDVDWDLINAGKEPVTLLTGGAFFHHGDSFAMMRGGHLDICVLGAFQ
                     VAANGDLANWHTGAPDAIPAVGGAMDLAIGAKKVFVMMEHQTKTGEAKIVERCSYPVT
                     GIGCVTRVYTDLAVIDVTPQGLKVVEMVDGLTLDELQKRTGAPLLHATK"
BASE COUNT          215 a          544 c          566 g          282 t
ORIGIN      
        1 ttcgcctgcc agatctcgac cagcgcgtcg ttcaccggct tgccgtcgcc gtcgagcacg
       61 cggccctgga tcgtgatcgg ctcgccggtc acgcccggtc cgacgagatc ctcgcggacg
      121 agccacgtga ggccgatgtg gaggtacggg ccgacggtct gggaggtggt tgcgtagagg
      181 ctcatggctc ctccctcact cgccggggct cgggtcctgc ggggtctcgt cgcgcccccg
      241 caggacgatg tcgaacttgt acccgagcgc gaactcggga accgtcgtct cccagtcgaa
      301 cagcgagacc aggcgcttcc gcgccttctc gtcggggacg cacaggtaca tcgggtcgta
      361 gtcgaggagc gggtcgcccg ggaagtacat ctgcgtcacg atgcgcgtcg cgaacgccgg
      421 tccgaacagc gagaagtgga tgtgcgccgg ccgccaggcg ttgtagtggt tgcgccacgg
      481 gtactcgccc ggccggatcg tggtgaaccg gtagcgcccc tcggcgtcgg tcagcgtgcg
      541 tcccgcgccc gagaaattcg ggtcgaccgg cgcgttgtgc tgatcgacgc ggtgcggata
      601 gcggccggac gagttcgcct gccagacctc gaccagcgtg tggggcaccg cgcggccgtt
      661 ctcgtcgaga acgcgcccgc tcacgatgat ccgctcgccg atcggctcgc ccttgtgctg
      721 cttcgtcagg tcggcgtcgc ccggcttgac ctcgtcgtgg ccgaacagcg gcccggtcag
      781 ctcggacagc gtctgcggga ggacgacgag cggtttcgtc ggcgcccgct tgacggtcga
      841 cgcgtacttc gggaagagat agtccggctg ggtaccgaca tagggccgcc gatagcccgt
      901 cgtctcgctc atgtacagac tccttggatc ttcctggcgg gcggttcggt tcgccgggcg
      961 tcgtgcgtcc cggtcgtcta tttcgtcgcg tgcaggagcg gcgcgccggt ccgcttctgc
     1021 agctcgtcga gcgtcaggcc gtcgaccatc tcgaccacct tgaggccctg cggggtgacg
     1081 tcgatcaccg cgaggtcggt gtacacccgg gtcacgcagc cgatgccggt gaccgggtag
     1141 gagcaccgct ccacgatctt ggcttcgccc gtcttcgtct ggtgctccat catgacgaac
     1201 accttcttcg cgccgatcgc gaggtccatc gccccgccga cggccgggat ggcgtccgga
     1261 gcgccggtgt gccagttcgc gaggtcgccg ttggcggcga cctggaacgc gccgaggacg
     1321 cagatgtcga ggtgcccgcc gcgcatcatc gcgaacgagt cgccgtggtg gaagaaggcg
     1381 ccgccggtga gcagcgtgac cggttccttc ccggcgttga tgaggtccca gtccacgtcg
     1441 tccttggccg gcgccggccc catgccgagc agcccgttct cgctctgcag gaagatctcg
     1501 cggtccttgg gcaggaagtt cgccacgcgc gtcggcaggc cgatgccgag gttgacgtac
     1561 gcgccctcgg gaatgtcgcg cgcgacgcgc gccgccatct ggtcacg
//
LOCUS       DBNHXO010000273         1806 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4441235, whole genome shotgun sequence.
ACCESSION   DBNHXO010000273 DBNHXO010000000
VERSION     DBNHXO010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4441235"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="AC162A_05760"
     CDS             <1..1087
                     /locus_tag="AC162A_05760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5010382.1"
                     /translation="LPVSPQFATGPLGASGLGFGIAIGMFSLGSLIARPVVGWWADRR
                     GRRLLLIGGAVLTAVALGLHLLVGALPGFVAVRLLLGVAEGAFLVAGLAMATDLAPPG
                     RTGEAVSLASLSIYGGVAIGPIIGDALLNAAGFSAVWILATVVAVVAALIAVAVPETR
                     PERAPGQAGGGGRLFHPAGVYPGILCLCATFGMAGFLTLIPLHTKAMGIGGAGPALAL
                     YGGIVMLIRFLGAKLPDRMGPVRLASMALFTTAAGLVLIGVLPGQTGLLIGTAVFAVG
                     VGLSIPSILAFAVSRASADERGSIVGTTSVFLDISFGLAPVALAPIADAAGIAATFVA
                     SALVAVLGAVLLLARRPVAQPATTPAG"
     gene            1098..>1806
                     /locus_tag="AC162A_05765"
     CDS             1098..>1806
                     /locus_tag="AC162A_05765"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5010383.1"
                     /translation="MSIERVFVAGAGLMGHGIAQVHAAIGKQVTLYEPDLARAVAGRD
                     RIAGNLELSVAKGRLTAEDRDATIARLRPSDDLATAGDADLLVEAVFEDVEVKSALWG
                     ALDAVAPVGALFASNTSSISIDALAAAVAPPRRAKFVGMHFFSPVPVIPLIELIRGTA
                     TDDATEAAVRALATDLGKQVIVTADRPGFIVNRILMPFLAEAMRAYEEGLGTAEDIDT
                     GARIGLNHPMGPLELADF"
BASE COUNT          190 a          661 c          665 g          290 t
ORIGIN      
        1 cctgccggtc tcgccccagt tcgcgaccgg cccgctcggg gcctcgggcc tcgggttcgg
       61 gatcgcgatc ggcatgttca gcctggggtc gctgatcgcg cggcccgtcg tcggctggtg
      121 ggcggaccgg cgcgggcgcc ggttgctgct catcggcggc gccgtgctga cggcggtggc
      181 gctcggcctc cacctgctgg tcggggcgct gcccggcttc gtcgcggtcc ggctgctgct
      241 tggcgtggcc gagggcgcgt tcctggtcgc tggcctggcc atggcgaccg acctcgcccc
      301 gcccggccgc acgggcgagg ccgtgagcct cgcgtcgctg tcaatctacg gcggcgtcgc
      361 gatcgggccg atcatcggtg acgcgctgct caacgccgcc gggttcagcg ccgtctggat
      421 cctcgcaacg gtcgtggccg tcgtggccgc gctgatcgct gtggcagtcc ccgagacgcg
      481 accggagcga gcgccgggcc aggccggcgg cggcggacgg ttgttccatc cggcgggcgt
      541 ctacccgggg atcctgtgcc tgtgcgcgac gttcggcatg gcggggttcc tgaccctgat
      601 cccgctccat accaaggcga tgggcatcgg cggggccggg cccgcgctcg cgttgtatgg
      661 cggcatcgtg atgctcatcc gcttcctggg cgcgaagctg ccggatcgca tgggcccggt
      721 gcgcctggcg tccatggcgc tgttcacgac cgcggccggg ctcgtgctga tcggcgtgct
      781 gcccggccag accgggctgc tgatcgggac ggccgtgttc gccgtcggcg tcggcctgtc
      841 gatcccgtcg atcctggcgt tcgccgtctc gcgggcgtcg gccgacgagc gcggctcgat
      901 cgtcggcacc acgagcgtgt tcctcgacat ctcgttcggc cttgcgccgg tcgccttggc
      961 gccgatcgcg gacgcggcgg gcatcgcggc gaccttcgtc gcgtccgcgc tggttgcggt
     1021 gctgggcgcc gtcctgctgc tcgcgcgccg gccggtcgcc caacccgcga cgaccccggc
     1081 ggggtaggct gctcgccgtg agcatcgagc gcgtgttcgt cgcgggagcg gggctgatgg
     1141 gccacgggat cgcacaagtg cacgccgcga tcggcaagca ggtcaccctc tatgagccgg
     1201 atctcgcccg ggccgtggcc ggccgggacc ggatcgccgg caacctcgag ctctcggttg
     1261 ccaagggccg gctgaccgcc gaggaccggg atgcgaccat cgcccgtctc cgcccgagcg
     1321 acgacctcgc gaccgccggc gacgcggacc tcctggtgga ggcggtgttc gaggacgtcg
     1381 aggtgaagtc cgcgctgtgg ggtgccctgg acgccgtcgc gcccgtcggc gcgctgttcg
     1441 cctccaacac ctcgtcgatc tcgatcgacg ccctcgcggc cgcggtcgcg ccgccgcgcc
     1501 gggcgaaatt cgtcggcatg cacttcttca gcccggtgcc ggtcatcccg ctcatcgagc
     1561 tgatccgcgg caccgccacc gacgacgcga ccgaggccgc cgtccgtgcg ctcgccaccg
     1621 acctcggcaa gcaggtcatc gtgacagccg atcggcccgg gttcatcgtc aaccgcatcc
     1681 tgatgccgtt cctcgccgag gccatgcgcg cctacgagga ggggctgggc accgccgagg
     1741 acatcgacac cggggcgagg atcgggctaa accacccgat ggggccgctc gagctcgccg
     1801 acttca
//
LOCUS       DBNHXO010000274         5246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4242362, whole genome shotgun sequence.
ACCESSION   DBNHXO010000274 DBNHXO010000000
VERSION     DBNHXO010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4242362"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1858)
                     /locus_tag="AC162A_05770"
     CDS             complement(2..1858)
                     /locus_tag="AC162A_05770"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR5010384.1"
                     /translation="MALTRSDPPTATGPRWEERFRASAILLSTIAIDAPDVGLATSNA
                     SGAPQLCRWNTATGELTQLTFDKGGRIVGRLTPDGRWVIWLRDAGGNEIGHWVAMPID
                     GGEPIDLSPQIAPYASDDVAFSRYGGRIAFTTASDDRFVVRVAALDAEGRVGELQTVH
                     HSAAALQSLAVCADGSRVAFGSAHRSTGLEFSLLTADTVRGDPGPELWDGPGTSIEVL
                     AFAPEHDDHRLVATTNASGRERAFVWDAVSGERRDLPAGAPGGDILALDWSPDGREVL
                     VCRIDQGEQRLLVWDLEADAIRALDHPTGAVIGYVRIMASYYRPDGAEIVCRWEDFGQ
                     PRRLIGLDPVTGRQTRTILAAGEVPAGRSLRSVAFPSADGTPLQAWLGVPDGDPPHPL
                     ILETHGGPTAATFATFHPQAQAFLDEGFAFLSLNYRGSTTFGREFEHAIWGRLGELEI
                     QDMAAARTWLIENGIAIPDQILLTGWSYGGYLTLLGLGRQPDLWAGGMAGIAVADWAM
                     NYEDSSDLLRAYQRMLFGGDPDEKAEAMRAGSPLTYVDDVRAPVLILQGRNDTRTPAR
                     PIERYVERLEARGHDVAIEWFEAGHLGGDDELSIRHQHLMVDFAKRVLAAGR"
     gene            1946..2611
                     /locus_tag="AC162A_05775"
     CDS             1946..2611
                     /locus_tag="AC162A_05775"
                     /inference="COORDINATES: protein motif:HMM:NF017959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HIR5010385.1"
                     /translation="MPRFDRATSGDLMWAGIVGPVLFVAVFLVDGAVRPGYDPVRLQV
                     SYLSLGDRGAVQIANFIVTGLLLGAFAVGLRRRLAPAGVRGARGAPIAVGSIALGLLI
                     AGVFSTAPAFGYPPGTPDGFPTSLPPTAYLHVVGALLFFGGMIATPALMARRFRAQGE
                     TAWTVYSVLTAVVVFVALAASSADPSGQPYLPATVGLLQRVSIAAGLAWIAALAAFAP
                     LDV"
     gene            2793..4502
                     /locus_tag="AC162A_05780"
     CDS             2793..4502
                     /locus_tag="AC162A_05780"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR5010386.1"
                     /translation="MSSQFRRSLVLCATAALLIATIGGSAPAVLGHGHGPGHGPRDTR
                     VAGIDVDAATIPELQRAMDRHRLSSFRLTLFYLHRIWKLNPKLNAVTKVSPTALGDAL
                     KADWARRRGSDLPLLGIPVLLKDNINTTGMPNTAGSWALAGSSPRDAFIVKQLRAAGA
                     VILGKTNLSEWANFRSTPSSSGWSGIGGQTNMAYAFDRNPCGSSSGSGVAASANLATI
                     TVGTETDGSIVCPAGANGDVGIKPTLGLLSRAGVIPISAEQDTAGPITRNVTDAAVML
                     GAMTGIDAKDAATAGQAGHAFTDYTQFLDKHALDGARIGVWREGTYDPTVSPDVDAIL
                     NQAIDVLESEGAVIVDPARIPIEDAYEPEFAALLCEFKSDIASYLETYTRPKYPKTLQ
                     DLIDFNDAHPGLEGPWNSAVFVDAEATGGRADPACTEAREAARSIAQAAIDDLMADHH
                     LDAIIAPTNGPAWLTDPVNGDLGGDFSTFVGSSSPSAIAGYATITVPAGFSSGLPIGV
                     SFIAGQWDEPELIGLAYDFEQASHARKPPKFLPTLGASAKHGHRVTDPADRTQPSRLP
                     PLR"
     gene            complement(4567..4836)
                     /locus_tag="AC162A_05785"
     CDS             complement(4567..4836)
                     /locus_tag="AC162A_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010387.1"
                     /translation="MSMRASLVETMHLAPDGDTVRRIQESGLAVADKETMSQAIHDVY
                     CGIMADHAEPNQKDLDQAQAMIDALRKIFLEQLAADQIAEEQPAS"
     gene            4980..>5246
                     /locus_tag="AC162A_05790"
     CDS             4980..>5246
                     /locus_tag="AC162A_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010388.1"
                     /translation="MTVVDWLLDSDPSIRWQVMSDLTDEPPEIIAAERARVAVEGWGA
                     RLLEVQAPDGQWGGRPWSHDWTDTFHVLELLRRFGLEPGSVQALR"
BASE COUNT          748 a         1872 c         1822 g          804 t
ORIGIN      
        1 ctcaccgacc ggcggcgagc acccgcttcg cgaagtcgac catcaggtgc tggtggcgga
       61 tcgagagctc gtcgtcgcca cccaggtgac cggcctcgaa ccactcgatc gcgacgtcgt
      121 ggccgcgtgc ctcgaggcgc tccacgtagc gctcgatcgg ccgcgccggc gtccgggtgt
      181 cgttgcggcc ctggaggatg aggacgggcg cccggacgtc gtccacatag gtcagcggtg
      241 atccggcgcg catcgcctcc gccttctcgt ccgggtcgcc gccgaacagc atccgctggt
      301 atgcccgcag cagatcgctc gagtcctcgt agttcatcgc ccagtcggcg accgcgatcc
      361 cggccatccc gccggcccac aggtccggct ggcggccgag cccgagcagc gtcaggtagc
      421 cgccgtacga ccagcccgtc aggaggatct gatccgggat cgcgatgccg ttctcgatca
      481 gccacgtccg cgctgcggcc atgtcctgga tctcgagctc gcccagccgg ccccagatcg
      541 catgctcgaa ctcccgcccg aaggtcgtcg agccgcggta gttgagcgag aggaacgcga
      601 agccctcgtc gaggaacgcc tgcgcctgcg gatggaaggt ggcgaacgtg gcggccgtcg
      661 ggccgccgtg ggtctcgagg atcagcgggt gcggcgggtc cccgtccggg acgccgagcc
      721 aggcctggag cggcgtgccg tccgccgacg ggaaggcgac gctgcgcagc gatcggccgg
      781 ccgggacctc gcccgcggcc aggatcgtcc gcgtctgacg cccggtgacg gggtcgaggc
      841 cgatcagccg ccggggctgg ccgaagtcct cccagcgaca cacgatctcc gcgccgtcgg
      901 gccggtagta ggacgccatg atccgcacgt acccgattac cgcacccgtc gggtggtcga
      961 gcgcacggat cgcgtccgcc tcgaggtccc agacgagcag ccgctgctcg ccctggtcga
     1021 tccgacagac caggacctcg cgcccgtcgg gtgaccagtc cagggcgagg atgtcgccgc
     1081 cgggggcgcc ggccggcagg tcgcggcgtt cgccggacac cgcgtcccac acgaaggctc
     1141 gctcccgacc cgaggcgttc gtcgtcgcga ccagacggtg atcgtcgtgc tcgggcgcga
     1201 acgcgaggac ctcgatgctg gtccccggcc cgtcccatag ctccgggcct ggatcgccgc
     1261 gcaccgtgtc ggccgtcaac aggctgaatt cgaggcccgt cgaacggtga gcggagccga
     1321 aggctacccg cgaaccgtcc gcgcaaacgg cgagcgattg gagggcggcg gcggagtgat
     1381 ggacggtctg gagctcgccg acgcggccct cggcgtcgag agcggcgacc cgcaccacga
     1441 agcgatcatc ggatgccgtg gtgaacgcga tccggccgcc gtagcggctg aacgcgacgt
     1501 cgtccgaggc atacggcgcg atctgggggc tcaggtcgat cggctctccc ccatcgatcg
     1561 gcatggccac ccaatggccg atctcgttgc cgcccgcatc gcgaagccag atgacccagc
     1621 gtccatccgg tgtcagccgc ccgacgatgc ggccaccctt gtcgaaggtc agctgcgtca
     1681 gctcgcccgt cgccgtgttc cagcgacaca gctggggggc gccgctggcg ttcgaggtgg
     1741 caaggccgac gtcgggtgcg tcgatggcga tcgtgctcag caggatcgcc gaggcgcgga
     1801 accgttcctc ccagcggggg ccggtcgcgg tcggcggatc ggacctggtc agcgccacgg
     1861 ccggacctcc ctgtgcgtgt catcgcatca tgcacggcgc caccgtcccc gtctcgccgg
     1921 gatcgcggtc tgtcaggatg ccccgatgcc gaggttcgat cgggcgacgt ccggcgacct
     1981 catgtgggca gggatcgtcg ggcccgtgct cttcgtcgcg gtgttcctgg tggatggtgc
     2041 cgtccgtccc gggtacgacc cggttcgcct ccaggtgagc tatctcagcc tcggtgaccg
     2101 cggcgcggtc cagatcgcca acttcatcgt caccggcctg cttctggggg cgttcgcggt
     2161 tgggttgcgg cggcggctgg caccggccgg agtccgcggt gcgcggggag cgccgatcgc
     2221 ggttggttcg atcgccctgg gcctgttgat cgccggcgtg ttcagcacgg cgccggcgtt
     2281 cggctatccg cccggcacgc ccgacggctt cccgaccagc ctgcccccga cggcatacct
     2341 ccacgtggtc ggcgcgcttc tcttcttcgg cggcatgatc gccacgccgg cgctgatggc
     2401 tcgacgcttc cgcgcccagg gcgagacggc ctggactgtg tactcggtcc tgacggcggt
     2461 cgttgtgttc gtcgcgctgg ccgcgtcgtc cgccgatccc agcgggcaac cgtacctgcc
     2521 ggccaccgtc ggcctgctgc agcgggtctc gatcgccgca ggtcttgcct ggatcgccgc
     2581 gctggcggcg ttcgcgcccc tggacgtctg aggctggagc aatcgcgagt gccgcgccca
     2641 aagcggtgcg ccggcgcaac actcacgacc gccgtcgcag acgccacgag tggcgccggc
     2701 ggggcggggg ttgacgccgg gggttccata gagggaggcc ccagtccccg ctacccgcgc
     2761 ccaccccaca tgtgcccgtg gaggtccgag cgatgagcag ccagtttcgg cgttccctcg
     2821 tcctgtgcgc gacggccgcg ctcctgatcg cgacgatcgg aggatctgcc ccggctgtgc
     2881 tcggccatgg tcacggccca gggcacggtc cgcgcgacac gcgcgtcgcc ggcatcgacg
     2941 tcgacgccgc gacgatcccg gagctccagc gggcgatgga ccgccaccgc ctgagctcgt
     3001 tccgtctgac gctgttctac ctccatcgca tctggaagct gaacccgaag ctgaacgcgg
     3061 tgacgaaggt gagcccgacg gcactggggg atgccctgaa ggcggactgg gcgcgccggc
     3121 gcggctctga cctccccttg ctcgggatcc ccgtgctgct caaggacaac atcaacacga
     3181 ccgggatgcc gaacaccgcg ggctcctggg ccctcgccgg cagtagcccg cgcgacgcgt
     3241 tcatcgtcaa gcagctccgc gccgcgggag cggtgatcct cggcaagacg aatctctcgg
     3301 agtgggcgaa cttccgatcc acgccgtcat cgagcggctg gagcggcatc ggcggccaga
     3361 cgaacatggc ctacgctttc gaccggaacc cgtgtggctc cagctccggg tccggcgtgg
     3421 cggcgtcggc caatctcgcg acgatcacgg tcggcaccga gaccgatggc tcgatcgtgt
     3481 gtccggccgg cgccaacggc gacgtgggca tcaagccgac gcttggcctg ctgagccggg
     3541 cgggggtcat cccgatctct gccgagcagg acaccgcggg gccgatcacg cgcaacgtga
     3601 ccgacgcggc ggtgatgctg ggcgcgatga ccggcatcga cgcgaaggat gccgcgaccg
     3661 ccggccaggc gggccacgcg ttcaccgact acacgcagtt cctcgacaag cacgcgctgg
     3721 acggtgcccg gatcggcgtc tggcgcgaag gcacctacga ccccaccgtc agccccgacg
     3781 tcgacgcgat cctgaaccag gcgatcgatg tgctcgagag cgagggcgcg gtcatcgtcg
     3841 acccggcgcg catcccgatc gaggacgcgt atgagcctga gttcgccgcg ctgctgtgcg
     3901 agttcaagag cgacatcgcc tcgtacctgg agacgtacac gcgcccgaag taccccaaga
     3961 cgctccagga cctgatcgac ttcaacgacg cccacccggg tctcgaaggc ccgtggaact
     4021 cggcagtgtt cgttgatgcc gaggcgaccg gtggccgtgc cgacccagcg tgcacggagg
     4081 cccgcgaggc cgcgcgatcg atcgcccagg ctgccatcga cgacctgatg gcggaccacc
     4141 atctcgacgc gatcatcgcg ccgaccaacg ggcctgcctg gctgaccgat ccggtcaacg
     4201 gcgacctggg cggcgacttc tcgacgttcg tcggctcgtc cagcccgtcg gcgatcgcgg
     4261 gttacgcgac gatcaccgtg cccgcgggct tctcctcggg cctgccgatc ggcgtctcgt
     4321 tcatcgccgg ccagtgggat gaacccgagc tcatcgggct cgcctacgac ttcgagcaag
     4381 cgtcgcatgc ccgcaagcca ccgaagttcc tgccgacgct cggcgcgtca gcgaagcacg
     4441 ggcatcgggt gaccgacccg gcggaccgca cgcagccgtc gaggctcccc ccgcttcgct
     4501 agagctctcg aaccaccgca acggacggcg gcaggtcctc cctgccgccg ttacgtcctg
     4561 tcaaggtcag gaggcgggct gctcctcggc gatctggtcg gccgcgagct gctccaggaa
     4621 gatcttccgc agggcgtcga tcatggcctg cgcctgatcc aggtccttct ggttcggctc
     4681 ggcatggtcg gccatgatcc cgcaatacac gtcgtggatc gcttggctca tggtctcctt
     4741 gtcggcgacc gccagaccgc tctcctggat ccgcctgacc gtgtcgccgt cgggcgccag
     4801 gtgcatcgtc tcgaccaacg acgctcgcat gctcatcgac ttctcctcgt ccggggccgc
     4861 gggacgcgaa gcctacgcca tcggcggcgt agagtcccgc gggattcatg acactcgtcg
     4921 tcatcaacga ggcggacact cggttcagtt gatcaccggc tgaggagcag ggggcgcgca
     4981 tgacggtcgt ggactggctg ttggattcgg acccgtcgat ccgctggcag gtcatgtcgg
     5041 acctgaccga cgagccgccc gagatcatcg cggccgagcg cgcgagggtc gccgttgagg
     5101 gttggggcgc ccggctcctc gaggtccagg cgccagacgg ccagtggggc ggccggccgt
     5161 ggtcgcacga ctggaccgac acgttccacg tgctcgagct gctgcggcgg ttcggccttg
     5221 agcccgggag tgttcaggcc ctccga
//
LOCUS       DBNHXO010000275         3672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_868940, whole genome shotgun sequence.
ACCESSION   DBNHXO010000275 DBNHXO010000000
VERSION     DBNHXO010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_868940"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="AC162A_05795"
     CDS             <1..854
                     /locus_tag="AC162A_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5010389.1"
                     /translation="GIPWDMGMIGFWYNKKLFADAGITAPPATWDEYLAAVKKLKDAG
                     ITPLAIAGKDMWPSMHLWTYLVLRQGGGEALAQMVQTGDWNTDACIKAGEAVVALNAL
                     EPYQAGYKGATYNEEAAAVGNGKAAMELMGQWAPSVQRDQSADKQGLGEDLGWFAFPA
                     ITGGAGAATDGVGGGNGIAVGKDAPPEAIDFLKYFMSVENQNKLNTDGIGLSTTVGTE
                     STVTDPNLQAVLAGRGAAQFMQLYLDQATTGTLGQTFNEATMALFAGASDPAAVCQAI
                     TDAAAAQ"
     gene            950..1888
                     /locus_tag="AC162A_05800"
     CDS             950..1888
                     /locus_tag="AC162A_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5010390.1"
                     /translation="MTALSARTRGNGSGARRSKSLTIALFLLPALALYVLFVLIPIVQ
                     AGHYSLYKWNGLQPLTDFIGLKNYETALSSAPFRAAIGNNVLIIVLSLAIQIPFSLAL
                     AVMLNRRFPGRAVFRLLFFLPYVLSEAVTGIVFRLLLQPDAFVDSAMSTVGLGALVQD
                     WFGDTNVVMITMFVIISWKYFGFHMILMLAGLQGIPKELEEAALIDGADKRQAFRHVT
                     LPLLGPTIRVSVFLSMIGALQLFDMIWVMTAGGPLNASNTMAISMFKAGFKGTQMGYG
                     SALAVILFLFSLVVALAYQRFVLRRDTEGALTTFGG"
     gene            1944..2795
                     /locus_tag="AC162A_05805"
     CDS             1944..2795
                     /locus_tag="AC162A_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010986387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5010391.1"
                     /translation="MGIRVARPSLLGIVAYIVLVAVAVAILIPIGYAVLGGFKTNGQL
                     AANPGQILPDPWVLENYEEVLVGKSAPTFWGEFGNSLLIAFISVTVAVVVASMAAFVF
                     ARMQFRGREALFTLFVFGLLFPTAVAILPLFILLRNLGLMGTPWGVALPQAAFALPLT
                     IVILRPFFRSIPAELEDAARIDGCSSFAFFWRVLLPLARPALATVSVLAVVTTWNAFL
                     LPLVVLNGAEQWTLPLGAQNFSTQYSSDQARVLAFTVVGIIPAIVFYAIAERQLVGGL
                     TSGAVKG"
     gene            2795..>3672
                     /locus_tag="AC162A_05810"
     CDS             2795..>3672
                     /locus_tag="AC162A_05810"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR5010392.1"
                     /translation="MTTTQARYRDPSLPIEQRVADLLARMTLDEKIAQLGSMWSFELF
                     RSETELDPALLRVRLGSGIGQISRVAGATNLDAAGAAAAGNEIQRFLVEETRLGIPAI
                     LHEETLHGLLARGAISFQQSIGAAAAFDPELVEAIAVTIRRRMLAIGGRLALAPVLDI
                     TRDPRWGRVEETYGEDPYLASALGVAYVRGMQGPDIATGVVATGKHLVGHGLAEGGLN
                     QAPAHAGPRELRDEQLLPFEAAVRDAGVACMMPAYCVVDGLPCHASEELLTSILRREW
                     GFDGIVASDYTAVQMLV"
BASE COUNT          537 a         1297 c         1226 g          612 t
ORIGIN      
        1 acggcatccc gtgggacatg ggcatgatcg gcttctggta caacaagaag ctgttcgccg
       61 atgcgggcat caccgcgccg ccggcgacct gggacgagta cctcgcggcc gtcaagaagc
      121 tcaaggacgc gggcatcacg ccgctcgcga tcgccggcaa ggacatgtgg ccgtcgatgc
      181 acctgtggac ctacctcgtg ctccgccagg gtggcggcga ggcgctggcc cagatggtcc
      241 agaccggcga ctggaacacc gacgcctgca tcaaggccgg tgaggccgtg gtcgctctga
      301 atgcgctcga gccgtaccag gccggctaca agggcgcgac ctacaacgag gaagcggccg
      361 cggtcggcaa cggcaaggcg gcgatggagc tgatgggcca gtgggccccg tccgtccagc
      421 gggaccagag cgcggacaag cagggcctcg gcgaggacct cggctggttc gccttcccgg
      481 ccatcaccgg cggggcgggc gcagcgaccg acggcgtcgg tggcggcaac ggcatcgcgg
      541 tcggcaagga cgcaccgccg gaggcgatcg acttcctgaa gtacttcatg agcgtcgaga
      601 accagaacaa gctcaacacc gacggcatcg gcctctcgac caccgtcggc accgagagca
      661 ccgtcaccga tcccaacctg caggccgtcc tcgccggtcg tggggctgcg cagttcatgc
      721 agctctatct cgaccaggcg acgaccggca cgctgggcca gacgttcaac gaggcgacga
      781 tggcgctgtt cgccggagcc tcggatcccg ccgcggtgtg ccaggcgatc accgacgccg
      841 cggccgccca gtaggaccga tctgcgagct cacggctcgt agactcgcaa cgggagcggg
      901 ccggccccag ccggcccgct cgattccacg gcgtgggtga aggatcctga tgacggcact
      961 gagcgcccgc acgcgcggga acggcagcgg ggcacgacgg tcgaagtcac tgacgatcgc
     1021 cctgttcctg ctgcccgcac tcgcgctgta cgtcctgttc gtgctcatcc ccatcgtcca
     1081 ggccgggcac tacagcctgt acaagtggaa cggcctccag ccgctcaccg acttcatcgg
     1141 gctcaagaac tacgagacag cgctgtcgag cgctcccttc agggcagcga tcggcaacaa
     1201 cgtgctgatc atcgtcctgt cgctggccat ccagatcccg ttctcgctgg cgctggcggt
     1261 catgctcaac cggcggttcc cggggcgggc cgtgttccgg ctcctgttct tcctgcccta
     1321 cgtgctgtcc gaggcggtca ccgggatcgt gttccggttg ctcctgcagc cggacgcatt
     1381 cgtggactcg gccatgtcca ccgttggcct gggcgccctc gtccaggact ggttcggcga
     1441 cacgaacgtc gtgatgatca ccatgttcgt gatcatctcg tggaaatact tcggcttcca
     1501 catgatcctc atgctggcgg gcctgcaggg gatccccaaa gagctcgagg aggcggcgct
     1561 gatcgacggc gccgacaagc gccaggcgtt ccgccacgtc acgctgccgc tgctcgggcc
     1621 gacgatccgg gtgtcggtct tcctgtcgat gatcggcgcg ctccagctgt tcgacatgat
     1681 ctgggtcatg acggccggcg gtccgctcaa cgcgtcgaac acgatggcga tctccatgtt
     1741 caaggccggc ttcaagggca cgcagatggg ctacggctcg gcgctcgcgg tgatcctgtt
     1801 cctgttcagc ctcgtggtcg ccctggcgta ccagcggttc gtgctccggc gcgacaccga
     1861 gggcgccctc acgacgttcg gtggctgacc cgatggcgac cacgacgacc aacgttggcg
     1921 ccccgtcgga tacggcccgg tggatgggca tccgcgtcgc ccgtccgagc ctgctcggga
     1981 tcgtcgccta catcgtcctc gtcgccgtgg ccgtggcgat cctcatcccg atcggctatg
     2041 cggtcctcgg cggcttcaag accaacggcc agctggcggc gaacccgggc cagatcctgc
     2101 ccgatccgtg ggtcctcgag aactacgagg aggtcctggt cggcaagagc gccccgacgt
     2161 tctgggggga gttcggcaac agcctgctga tcgccttcat ctcagtcacg gtcgccgtgg
     2221 tggtcgcgtc gatggcggca ttcgtgttcg cccggatgca gttccgcggc cgcgaggccc
     2281 tgttcacgct gttcgtgttc gggctgttgt tcccgacggc cgttgcgatc ctgccgctgt
     2341 tcatcctgtt gcggaacctc ggcctcatgg gcacgccatg gggcgtggcc ctgccacagg
     2401 cggcgttcgc gctgccgctc acgatcgtca ttctgcgccc gttcttccgg agcatccctg
     2461 cggagctcga ggacgccgcg cggatcgacg gctgcagctc gttcgccttc ttctggcgcg
     2521 tcctgctgcc cctggcgcgg cccgccctcg cgaccgtcag cgtcctcgcc gtggttacga
     2581 cctggaacgc attcctgcta ccgctcgtcg tgctcaacgg cgcggagcag tggacccttc
     2641 cgctcggcgc ccagaacttc tcgacccagt actcatccga ccaggcgcgc gtcctcgcgt
     2701 tcaccgtggt cgggatcatc ccggcgatcg tcttctacgc gatcgccgag cgccagctgg
     2761 tgggcggcct cacgtccggg gcggtgaagg gctgatgacg accacccagg cacgctaccg
     2821 cgaccccagc ctgcccatcg agcagcgcgt ggcggacctg ctcgcacgca tgacgctcga
     2881 cgaaaagatc gcccagctcg ggtcgatgtg gtcgttcgag ctgttccgga gcgagaccga
     2941 gctggaccct gccctcctcc gggtccggct cggctccgga atcggtcaga tcagccgcgt
     3001 cgccggcgcg accaacctcg acgcggcggg cgcggccgcg gccggcaacg agatccagcg
     3061 gttcctggtc gaggagacgc gcctcgggat cccggccatc ctccacgagg agacgctcca
     3121 cggcctgctc gcgcgcgggg cgatctcgtt ccagcagtcg atcggtgccg cggccgcgtt
     3181 cgaccccgag ctggtcgaag cgatcgccgt cacgatccgc cggcgcatgc tcgcgatcgg
     3241 cgggcgcctg gcgctcgccc ccgtcctcga catcacccgc gacccacgct ggggccgcgt
     3301 ggaggagacc tacggcgaag acccgtatct cgccagcgca ctcggcgtgg cctacgtgcg
     3361 cggcatgcag ggtcccgaca tcgccacggg ggtcgtcgcg acgggcaagc atctcgtggg
     3421 ccacgggctc gccgagggtg gtctcaacca ggccccggcc cacgccggtc ctcgcgagct
     3481 gcgcgacgag cagctgctgc cgttcgaggc ggccgtccgc gatgccggcg tggcatgcat
     3541 gatgcccgcc tattgcgtcg tcgatggcct cccgtgccac gcgtcggagg agctgctgac
     3601 ctcgatcctg cgccgcgagt ggggctttga cgggatcgtc gcgtccgact acaccgcggt
     3661 ccagatgctg gt
//
LOCUS       DBNHXO010000276         3397 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4838828, whole genome shotgun sequence.
ACCESSION   DBNHXO010000276 DBNHXO010000000
VERSION     DBNHXO010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4838828"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="AC162A_05815"
     CDS             <1..921
                     /locus_tag="AC162A_05815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5010393.1"
                     /translation="IWGGDDGAHVLDFVRALRRPVVTTLHTVLRHPTPAQRRILTTLV
                     GMSASTVVMSQAAASMLTRAYGVDESRLEIVPHGVPDLPLVDPSTMKPRLGLEGRMVI
                     LSFGLLGPGKGYESAIAAMPAVVAADPSALYVILGATHPDLLLREGEAYRRSLEAKAA
                     AVGMTDHVRFIDRFVGRLELGTWLEAADIFVTPFPNHDQIVSGTLSYAMSAGRAIVST
                     PYAYARERLAKGRGCLVAAGSSDALATAFVELLADPARRASYGRRAYEYSRGMVWSTV
                     GAEYGRILARAALPVLPPLRSVAKLAAVGG"
     gene            962..2047
                     /locus_tag="AC162A_05820"
     CDS             962..2047
                     /locus_tag="AC162A_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010394.1"
                     /translation="MTGELGIWQHAIGPLPNEAFGYCTDDVARALTVDLDHARVLGWP
                     AVETSAWRSVHFMRDAFDPATGRFRNFRAADGHWLDGDASQDSYGRAMLALGGAMRAR
                     TDDDFAARARLLFAVALPGASRLTALRATASTLLGCAAVLESADVDGALRDSTTEAVE
                     QLSTRLGAAFAPSAHDADWPWPEPVLTYENALLPRALIVAGRKHRDHELIRLGLLTLD
                     WLMDVQTSDKGRFSPIGSSGFWPRGGPRARFDQQPIEATAMILAAEAAFAQTDEPRYL
                     EAVDRAYGWFLGDNDVGLALADPVTGSCFDGLEPAGVNLNQGAESTLMWLTALEHVRE
                     IRGAASAQLATRTPARPAVATVSTSRG"
     gene            2053..3036
                     /locus_tag="AC162A_05825"
     CDS             2053..3036
                     /locus_tag="AC162A_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HIR5010395.1"
                     /translation="MHPDAAGLFQRHPLNPIISVDQLPYRANSVFNPGAARVGDETVL
                     LLRVEDLRGISHLAVARSRDGVSDWRFDAVPLLAPQPDHNPEEIWGCEDPRLTWLPER
                     EDWAIAYTAYSRRGPLVSLAMTRDFVEVRRLGPAMPPEDKDAALFPRRFDGRWAMIHR
                     PSPLRGGAHMWISYSPDLQHWGDHSLLLEARDGAWWDAGKIGLGPPPLETRDGWLLCY
                     HGVHVTAGGPIYRIGFALLDLDDPRIVLRRTDDWMFGPSAPYERDGDVNKVVFPTGWV
                     HDAETGILSMYYGAADSHVALATARLDELLEYMAHAPRPELRRSDDHPGSW"
     gene            complement(3064..>3397)
                     /locus_tag="AC162A_05830"
     CDS             complement(3064..>3397)
                     /locus_tag="AC162A_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010396.1"
                     /translation="FGRMPPVRRLSNTIGTAAFSWAVGQHVPDNQSGYRLLGRRLMTA
                     MLDSRETGFEFEVEMIAVCIRNGWTMEWVPISTIYAGEPSPIRPVAHLRHFIRATRAA
                     RRLVREGA"
BASE COUNT          474 a         1238 c         1183 g          502 t
ORIGIN      
        1 atctggggcg gcgacgacgg cgcccacgtc ctcgacttcg tccgggcgct ccggcgcccc
       61 gtcgtgacga cgctccacac cgtgctccgg cacccgacgc ccgcccagcg gcgaatcctc
      121 acgacgctcg tcggcatgtc ggcgtcgacg gtcgtgatgt cccaggcagc ggcctcgatg
      181 ctgacccgcg cgtacggcgt cgacgagtcc cgcctcgaga tcgttcccca cggcgtcccc
      241 gacctgcccc tcgtcgaccc cagcacgatg aagccccgcc tcggcctcga gggccgcatg
      301 gtgatcctca gcttcgggct gctcggaccc ggcaagggct acgagtctgc gatcgcggcg
      361 atgcccgccg tcgttgccgc cgatccctcc gcgctctacg tgatcctggg tgcgacgcac
      421 ccggacctgc tgctgcgcga aggcgaggcg taccgccgga gcctggaggc gaaggccgcg
      481 gccgtcggca tgaccgacca cgtccgattc atcgaccggt tcgtcggacg tctcgagctc
      541 ggaacgtggc tcgaggccgc cgacatcttc gtcaccccat tcccgaacca cgaccagatc
      601 gtgtctggga cgctgtccta cgcgatgagc gccggcaggg cgatcgtctc gacgccctac
      661 gcctacgccc gcgaacgcct ggccaaggga cgcggctgcc tcgtggcggc gggctcgtcg
      721 gacgcgctcg caacggcatt cgtcgagctg ctcgctgatc ccgcgcgacg cgcgtcgtat
      781 ggacggcgag cgtacgagta cagccgggga atggtctggt ccaccgtggg ggcggagtac
      841 ggccggatcc ttgcccgtgc ggcgttgccc gtgttgccac ccctgcggtc ggtcgcgaag
      901 ctcgcggccg taggtggctg accacccgct ccatcccgtc agccgggatc accttcgggc
      961 gatgaccggg gagctcggga tctggcagca cgccatcgga ccgctcccca acgaggcgtt
     1021 cgggtattgc accgacgacg tggcccgcgc cctcaccgtc gacctcgacc acgcacgggt
     1081 cctcggctgg ccggccgtcg agacgagcgc ctggcgctcc gtgcacttca tgcgcgacgc
     1141 cttcgatccg gcgaccgggc ggttccggaa cttccgcgcc gcggacggcc actggctgga
     1201 cggagatgcg tcgcaggaca gctatggccg agcgatgctt gcgctcggcg gcgcgatgcg
     1261 tgcacggacc gacgacgact tcgccgcccg cgcgaggctg ctgttcgccg tcgcgctgcc
     1321 gggagcgtcg cgactgacgg ccctccgcgc cacggcgtca acgctcctcg gttgcgccgc
     1381 cgtgctcgag tccgcggacg ttgacggcgc gctccgcgac tcgacaaccg aggccgtaga
     1441 acagctgagc acccggctgg gagcggcctt cgcgcccagc gcgcacgacg cggactggcc
     1501 gtggccggaa cccgtcctca cgtacgagaa cgccctgctc cctcgcgccc tcatcgtcgc
     1561 cggcaggaag caccgcgacc acgagctcat tcgcctcgga ctgctcacgc tcgactggct
     1621 gatggacgtc cagacctccg acaaggggcg gttctcgccc atcggcagca gcgggttctg
     1681 gccacgcggc gggccgaggg cccggttcga ccagcagccg atcgaggcga cggcgatgat
     1741 cctggccgcc gaggcggcct tcgcccagac cgatgagccg cgctacctcg aggcggtcga
     1801 ccgcgcctac ggctggttcc tcggcgacaa cgacgtcggg ctcgctctgg ccgatccggt
     1861 cacgggcagc tgcttcgacg ggctcgagcc ggcaggggtg aacctgaacc agggcgccga
     1921 gtcgacgctc atgtggctga ccgcgctgga acacgtccgt gagatccgtg gcgccgcgag
     1981 cgcgcagctc gccacgcgga cgccggcccg gccggcggtc gcgaccgtgt caaccagccg
     2041 agggtgatgc cgatgcatcc cgacgctgcg ggcctgttcc agcgccatcc gttgaacccg
     2101 atcatctccg tcgaccagct gccgtatcgg gccaactccg tgttcaaccc cggggcggca
     2161 cgcgtcggcg acgaaacggt gctcctgctg cgcgtcgagg acctgcgtgg gatttcgcat
     2221 ctcgcggtcg cgcggagccg ggacggcgtc tcggactggc gtttcgatgc cgtgccgctg
     2281 ctcgcgcccc agccggatca caacccggag gagatctggg gctgcgagga tccgcggctg
     2341 acctggctcc cggaacgcga ggactgggcg atcgcctaca cggcctacag ccgccgcggg
     2401 ccgctagtgt cgctcgcgat gacgcgcgac ttcgtggagg tccgccgcct cggccccgcg
     2461 atgccgcccg aggacaagga cgccgcgctg tttccgcgcc ggttcgacgg ccgctgggcg
     2521 atgatccatc gaccctcgcc gctgcgcggc ggcgcgcaca tgtggatctc gtactcgccg
     2581 gacctgcagc actggggcga ccacagcctg ctgctcgagg cgcgcgatgg cgcatggtgg
     2641 gatgcgggca agatcggcct cgggcctccg ccgctcgaga cgagggacgg ctggctgctc
     2701 tgttaccacg gcgttcacgt caccgcgggc gggccgatct accggatcgg gttcgcgctg
     2761 ctcgacctgg acgatccacg aatcgtgctt cgccggacgg atgactggat gttcggaccc
     2821 tcggcgccgt atgagcggga cggcgacgtg aacaaggtcg tcttcccgac cggctgggtg
     2881 cacgatgccg agacggggat cctctcgatg tactacggcg cggccgacag ccacgtcgcg
     2941 ctcgccacag cgcgcctgga cgagctgctc gagtacatgg cccacgctcc gcggcccgag
     3001 ctgcggaggt ccgacgacca ccccggcagc tggtagcgcc gggggaaccc tggtccggcg
     3061 ggtctacgcg ccctcgcgga cgagccgccg ggcggcacgc gtcgcgcgga tgaagtggcg
     3121 caggtgggcc accggccgga tggggctggg ctcgccggca tagatggtcg agatcgggac
     3181 ccactccatc gtccagccgt tgcggatgca cacggcgatc atctcgacct cgaactcgaa
     3241 gcccgtctcg cggctgtcga gcatcgccgt catcagccgg cgtccgagga gtcgatagcc
     3301 cgactggttg tcgggcacgt gctgtccgac ggcccatgag aacgcggccg tcccgatcgt
     3361 gttggacagg cggcggaccg gcggcatgcg cccgaag
//
LOCUS       DBNHXO010000277         3429 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1662897, whole genome shotgun sequence.
ACCESSION   DBNHXO010000277 DBNHXO010000000
VERSION     DBNHXO010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1662897"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2739
                     /locus_tag="AC162A_05835"
     CDS             <1..2739
                     /locus_tag="AC162A_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HIR5010397.1"
                     /translation="DRDKDGVPVPDRGLLVLPEPSEGDLFLDLEGDPFALDDGVDYLF
                     GILEPRLSEEGRWVAADGARVPRFHAIWSLDDDGLVTWASEKAAFERTIDLVMDRWAQ
                     DPAMHVYHYAAYERTALGRLAQRHGTREEEVDRLLRGRVLVDLFRVVRQGIRAGVESY
                     SIKRIEPLYALHREIELKDAGSSIVAFETWLELGPDAPVENGRAILAGIEDYNRDDVV
                     SNWRLRDWLEERRRDLEAQEGVVLPRPGSDDGAATEALTAREEAVAELSASLTVDDPG
                     DPVLRAQSPEVAGRWLLAQLLSWHRREEKSSWWRYYDLMGRTDEELIAEREPLGGLVP
                     LDAWPVKKSIYHRFSFPAQDYRIGEDTDIDDPRTKKSAGEVVAIDNLASTITIRRGPI
                     LAAEALPRSIVPRGIVPSKELAESLLRTGQWVAAHGLGEVDPDSPDAAGMAAVRAMLM
                     RRPPSAGQGPGGPLKRAGESPLDAARRIVLSAPGGVLPIQGPPGSGKTYTGARMIVAL
                     AQQGKRVGVVANSHKVIGKVLDEVAIAAREAAVTVRIGQKPKTDQAPTHAAAEVLAAN
                     ANVEEALRTHAVDVVGAVAWTWCRPEMARPEPAVDVLVVDEAGQMSLANVLACAPAAR
                     MVVLLGDPQQLDQPTQGAHPPGAERSALSHLLADPAYVHPDRPTITPAEGLFLDQTWR
                     LHPDVCRFTSEAFYAGRLRPVEGLERQLIVGGTGATAASAGLLSGTGLRYLPVPHAGN
                     ATDSAEEAAVIANLVADLLAAGTHWVDRAGVEHELRVSDVVIVAPYNAHVGAIERQCA
                     EIGLPPVFAGTVDKFQGQEAPISIYAMGTSTPEEAPRGMEFLYSLNRLNVATSRARCV
                     AAIVMSPALVRVACRTPRQMRLANGLCLAVEAAGEVAAAGSTAEPLLVLFPHLDQG"
     gene            2755..>3429
                     /locus_tag="AC162A_05840"
     CDS             2755..>3429
                     /locus_tag="AC162A_05840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5010398.1"
                     /translation="MTQPAGPALDADRRHVRNALVAGVALGSTGHIAAVTVGTIVAAQ
                     MLGTQALAGAPAATVVLGAAVGSIVLSGLMARIGRRRIGLAAGYGIGVLGALIAAAAV
                     FTNSFPLLVCGTLLIGFGNSSNQLSRYAAADMVPPERRASAIGIVVWGATVGSVIGPT
                     LVPISGDLAVQAGLPALVGPYLVPVLFVGLAAILSFALLRPDPYELADEASRRATRED
                     PGPSVGP"
BASE COUNT          473 a         1218 c         1261 g          477 t
ORIGIN      
        1 gatcgcgaca aggacggggt cccggtgccg gaccgcgggc tgctcgtgct cccggaaccc
       61 agcgagggcg acctgttcct cgacctcgag ggcgacccgt tcgcgctcga cgacggcgtc
      121 gactacctgt tcgggatcct cgagccgcgg ctgtccgagg aagggcgctg ggtggccgcc
      181 gacggggccc gggtacccag gttccacgcg atctggagcc tcgacgacga cggcctggtc
      241 acctgggcat ccgagaaggc cgccttcgag cggacgatcg acctggtcat ggaccgctgg
      301 gcgcaggacc cggcgatgca cgtctaccac tacgcggcgt acgagcggac cgcgctcggc
      361 cgcctggcgc agcgccatgg cacccgcgag gaggaggtcg atcggctgct gcgcggccgg
      421 gtgctggtgg acctgttccg ggtcgtccgc cagggcatcc gggccggggt cgagagctac
      481 tcgatcaagc ggatcgagcc gctgtacgcc ctccatcgcg agatcgagct caaggacgcc
      541 ggcagcagca tcgtggcgtt cgagacgtgg ctcgagctgg gtcccgacgc gccggtcgag
      601 aacgggcggg cgatcctggc cgggatcgag gactacaacc gcgacgacgt cgtgagcaac
      661 tggcgtctcc gcgactggct cgaagagcgg cggcgcgacc tcgaggcgca ggaaggcgtc
      721 gtgctccctc gtccgggaag cgatgacggc gcggcgaccg aggcgctgac cgcgcgcgag
      781 gaggcggtcg cagagctgtc ggcgtcgctc accgtcgacg atccgggcga tcccgtcctg
      841 cgggcccagt cgcccgaggt cgcgggccgc tggctgctcg cccagctgct ttcgtggcac
      901 cggcgcgagg agaagtcgtc ctggtggcgc tactacgacc tgatgggccg gaccgatgag
      961 gagctgatcg cggaaaggga gccgctgggc gggctcgtgc cgctcgacgc ctggcccgtc
     1021 aagaagtcca tctaccaccg gttctcgttt ccggcgcagg actaccggat cggcgaggac
     1081 acggacatcg acgatccccg gacgaagaag tccgccgggg aggtcgtcgc gatcgacaac
     1141 ctcgcgagca cgatcacgat ccgccgggga ccgatcctgg ccgcggaggc gttgcctcga
     1201 tcgatcgtcc cccgcggcat cgttcccagc aaggagctgg ccgagagcct gcttcgaacc
     1261 ggccagtggg tggccgccca cggccttggc gaggtcgacc ccgattcgcc ggatgccgcc
     1321 ggcatggccg ccgtgcgcgc catgctcatg cggaggccgc cgagcgccgg tcagggtccg
     1381 ggcgggcccc tgaagcgcgc cggcgagtcg ccgctcgatg cggccaggcg gatcgtcctg
     1441 agcgcgcccg ggggcgtgct gccgatccag ggaccaccgg gctcgggcaa gacctacacc
     1501 ggggcgcgga tgatcgtcgc cctcgcgcag cagggcaagc gcgtcggcgt cgtcgccaac
     1561 agccacaagg tcatcggcaa ggtgctcgac gaggtcgcca tcgccgctcg ggaggcggcc
     1621 gtcaccgtcc gcatcggcca gaagcccaag accgaccagg ctccgacgca cgcggccgcg
     1681 gaggtgctcg cggcgaacgc gaacgtcgag gaggcgctgc ggacccacgc ggtcgatgtg
     1741 gtcggggccg tcgcctggac gtggtgccgc ccggagatgg cacgccccga acccgcggtc
     1801 gacgtgctcg tggtcgacga ggccggccag atgagcctgg ccaacgtcct cgcgtgtgcg
     1861 ccggcggcac gaatggtcgt gctgctcggc gacccgcaac agctcgacca gccgacccag
     1921 ggcgcacacc caccgggggc ggagcgcagc gccctgtcgc acctgctcgc cgacccggcg
     1981 tacgtgcacc cggaccggcc cacgatcacc cccgccgagg gcctgttcct cgaccagacg
     2041 tggcgcctgc atcccgatgt ctgcaggttc acgtcggagg ccttctacgc cggacgactg
     2101 cgtccggtcg agggtctcga gcgccagctg atcgtcggtg gtaccggcgc taccgcggcc
     2161 tcagccgggc tcctgtcggg cacggggctc cgctacctcc cggtacccca cgccggcaat
     2221 gcgaccgact cggcggaaga ggcggcggtc atcgcgaacc tcgtggctga cctccttgcc
     2281 gcgggcacgc actgggtcga ccgagcgggc gtcgagcacg agctcagggt gagcgacgtc
     2341 gtgatcgtcg cgccgtacaa cgcgcatgtc ggggcgatcg aacgccagtg cgccgagatc
     2401 ggcctgccgc cggtcttcgc gggcaccgtg gacaagttcc agggtcagga agccccgatc
     2461 agcatctacg cgatgggcac gtccacgccc gaggaggcgc cgcgtggcat ggagttcctg
     2521 tactcgctga accgcctcaa cgtcgcgacc tcgcgcgccc gctgcgttgc cgcgatcgtg
     2581 atgtcgccgg cgctggtgcg cgtcgcgtgc cggacgccgc ggcaaatgcg cctggcgaac
     2641 ggcctgtgcc tcgcggtcga ggctgccggc gaggtggcgg ccgcgggatc gacggccgag
     2701 ccgctgctgg tcctgttccc ccacctcgac cagggctgac gtatcgtcgt ggcgatgaca
     2761 caacccgcgg gccccgcgct cgacgccgac cgcaggcacg tccgcaacgc gctcgtcgcg
     2821 ggcgtcgcgc tcgggagcac ggggcacatc gccgcggtga cggtcgggac gatcgtcgcg
     2881 gcgcagatgc tcgggacgca ggccctcgcg ggagcaccgg cggcgaccgt cgtccttggc
     2941 gcggcggtcg ggtcgatcgt cctgtcgggc ctgatggccc gcatcgggcg ccgccgcatt
     3001 gggctggccg ccggctacgg catcggcgtg ctcggagcgc tgatcgcggc ggctgcggtg
     3061 ttcaccaact cgttcccgct cctcgtgtgc ggaacgctcc tcatcggctt cggcaacagc
     3121 tccaaccagc tgtcgcgcta cgcggccgct gacatggtgc cgccggaacg gcgtgcatcg
     3181 gccatcggca tcgtcgtctg gggagcaacg gtggggtcgg tcatcggccc gacgctggtc
     3241 ccgatctcgg gcgacctggc cgtgcaggcc gggctgccgg cgctcgttgg gccctacctc
     3301 gtgcccgtgc tgttcgtggg cctcgcggcg atcctctcgt ttgccctgct gcggccggac
     3361 ccgtacgagc ttgcggacga ggcctcccgg cgggcgacac gcgaggatcc gggcccaagc
     3421 gtcgggccc
//
LOCUS       DBNHXO010000278         1550 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1467030, whole genome shotgun sequence.
ACCESSION   DBNHXO010000278 DBNHXO010000000
VERSION     DBNHXO010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1467030"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(435..1217)
                     /locus_tag="AC162A_05845"
     CDS             complement(435..1217)
                     /locus_tag="AC162A_05845"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR5010399.1"
                     /translation="MAAERISKVLAAAGVASRRGADELVSAGRVTVDGRAAVLGEKVD
                     PSEQSIAVDGRPIRALDSRPLYLVMHKPAGVTSTVRDRHAATTVLDLVPADLARGGRL
                     YPVGRLDQDSEGLILLTNDGDWAEHVLHPRYEVEREYAVGLSRPLTREQAEALEAGIE
                     LTEGVATVTGLRGQTNTEDRLLAELLEPAPDPHGTWVRVTIHQGWKRQLRRMFGEVGA
                     PVRRLVRVRIGSLRLGDLAAGDVRPLTASEVRRLGSTGRADG"
     gene            complement(1221..>1550)
                     /gene="scpB"
                     /locus_tag="AC162A_05850"
     CDS             complement(1221..>1550)
                     /gene="scpB"
                     /locus_tag="AC162A_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR5010400.1"
                     /translation="AALETLAIVAYRQPVTRGAIERIRGVDSDYTVRALLHRRLIVEL
                     GRSDAPGRPYVYGTGFEFLERFGMTSLEELPPLDIDVATRLVDEADEGGGSAGAVPVI
                     AVDDDAT"
BASE COUNT          214 a          569 c          561 g          206 t
ORIGIN      
        1 accgcagacc cagccggccg gcggcggcgg cacccacgct gctcttgccg ctcgacgcgg
       61 gcccatcgag cgcgacgacg atccgccgcc cggtggcggg cttcgcggct gcggatcggg
      121 aacgcgacga tttggcgggc gcggccatcg ttcgcgagca taccggccac ggatcgcagc
      181 gaccaaggat ccgagatcgc cggcaccgcc gttggcgccc atgactccct cgaaaccgtc
      241 aaccagtcaa aggacgactg ttcgacgccg gttcggtcag ccgtggagcc gctatcgacg
      301 cgagcccgcg acgaagtagc cggtagcggc taacagttgc gttgctggcg ccgggtccat
      361 cgtctaccag tcgtgctttg actgcatggc ggaaacgggc gggtgatgac ggtcgatcgg
      421 gcggtgccgc ggcgtcagcc gtcggcccgg ccggtggagc cgagccgcct gacctccgag
      481 gccgtgagcg gccgcacgtc gcccgccgcg aggtcgccga ggcgcagcga cccgatccgg
      541 acccggacga ggcgacggac gggcgcgccc acctcgccga acatccgccg cagctgacgc
      601 ttccagccct ggtggatcgt cacccggacc caggtgccgt gcggatcggg ggccggttcg
      661 aggagctcgg ccagcagccg gtcctcggtg ttggtctggc cccgcagccc ggtcaccgtc
      721 gcgacgccct cggtgagctc gatccccgcc tcgagcgcct cggcctgctc gcgcgtcagc
      781 ggccgcgaca gtcccaccgc gtattcccgt tcgacctcgt agcgcgggtg gagcacgtgc
      841 tcggcccagt cgccgtcgtt ggtcagcagg atcaggccct cggagtcctg gtcgagccgg
      901 cccactgggt agaggcgccc tccccgggcg aggtcggccg ggacgaggtc gagcacggtg
      961 gtcgcggcat gccggtcacg gacggtcgag gtcacgcccg ccggcttgtg catgacgagg
     1021 tagagcggtc gcgagtccag cgcccggatc ggccggccat cgaccgcgat cgactgctcg
     1081 gacggatcga ccttctcgcc caggaccgcc gcgcgtccgt cgacggtgac ccggcccgcg
     1141 gaaaccagct cgtcggcccc ccgccgggag gcgacgcccg cggccgcgag gaccttggag
     1201 atccgctcgg ccgccatcgg tcaggtcgcg tcgtcgtcga cggcgatcac cggcaccgcg
     1261 cctgcggagc cgcctccttc gtcggcctcg tccaccaggc gcgtcgccac gtcgatgtcg
     1321 agcggtggca actcctcgag cgaggtcatc ccgaaccgct cgaggaactc gaacccggtc
     1381 ccgtacacgt aggggcgccc gggcgcatcg gaccggccga gctcgacgat cagccggcgg
     1441 tggagcagcg cgcggaccgt gtagtcggag tcgacgccgc ggatgcgctc gatggcgccg
     1501 cgggtgaccg gctgccgata ggcgacgatc gcgagcgtct cgagcgccgc
//
LOCUS       DBNHXO010000279         2489 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2060180, whole genome shotgun sequence.
ACCESSION   DBNHXO010000279 DBNHXO010000000
VERSION     DBNHXO010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2060180"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..488)
                     /locus_tag="AC162A_05855"
     CDS             complement(150..488)
                     /locus_tag="AC162A_05855"
                     /inference="COORDINATES: protein motif:HMM:NF017273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIR5010401.1"
                     /translation="MSLDLLGLAVLMAAVTYPSRALPLLTPGIERLPPRALLYLRLVG
                     PAVLASLAAVGALVLTLPDGSRQIHVGVEVVGVALCIAIVAWRRNLLLGLVVGVGFVA
                     VTRALGVPLT"
     gene            complement(485..1315)
                     /locus_tag="AC162A_05860"
     CDS             complement(485..1315)
                     /locus_tag="AC162A_05860"
                     /inference="COORDINATES: protein motif:HMM:NF015549.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR5010402.1"
                     /translation="MTDPAPLLDLRAGRRRLLLDGAGITLSAVGFALVYGLAARGAGY
                     SLVEAMAMSVIVFAGASQFVAVGYVAAGLPWLPIVVVTFFLNLRHALYSASLAPRVRH
                     VSAGQRAAMAHVLTDEAFALSAAHFQRIGRVDVPGYWIAAVVLVFIPWNLATIAGVLL
                     GDAIPNPRTLGLDVVFPAAMGGLAVGLVTGRRELVAAGAGAAIGVVASLAVGTEIGII
                     AGGLLGPLVAMAVPDGGRHVVGDSDLTGRPVDVDEAMQELVDLGATPADAADDAGPRA
                     "
     gene            complement(1344..1616)
                     /locus_tag="AC162A_05865"
     CDS             complement(1344..1616)
                     /locus_tag="AC162A_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010403.1"
                     /translation="MTSNVGQNYPYTSETETDRAQRVASLVAEREGLADTLSAETTPL
                     DTNERWWVWKCPTAGCPGLLHAAGYAHEKHAVFVVCDGTCAKTFLR"
     gene            complement(1758..>2489)
                     /locus_tag="AC162A_05870"
     CDS             complement(1758..>2489)
                     /locus_tag="AC162A_05870"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR5010404.1"
                     /translation="RDAYLADPSFHDVPVERLLSADHASELAARIDPSRAEPAPTPVR
                     TLVGGTIYLAVVDAAGNAVSLIQSNASGFGSGVLDPETGVHFQNRGASFSLDPLSPNV
                     LAPGKRPAHTLLPGMLFREGERRPWVVAGSMGGDNQPQIHAQLVSALVDGGADIATAI
                     AAPRVIVEPTGMQAPTLVALTDGDLAPGVADGLAQRGHDLRRVAYDGSLGHEHAIELI
                     DGGPAGDGTLAAAADPRSFGLPAVR"
BASE COUNT          371 a          906 c          924 g          288 t
ORIGIN      
        1 ccgcggatgc cgattccgta gccgcggtcc atggcgaggt gttcaagcac gtgcgccctg
       61 cggcgaccct cgtcgtggtc gcgcgcctga tgacgccgag cctgctggtg gagatcgagg
      121 cggacgcccg caagggatag gcccccggct caggtcagcg ggacgccgag tgcgcgggtg
      181 acggccacga agccgacccc gacgaccagt cccagcagca ggtttcgccg ccaggccacg
      241 atcgcgatgc acagggcgac cccgacgacc tcgacgccga catggatctg gcgcgagccg
      301 tccggcaggg tcaggaccag cgcgcctacg gcagccagcg atgcgagaac cgcggggccg
      361 acgaggcgca ggtagaggag tgcgcgcggc ggcagccgct cgatccccgg cgtcagcagc
      421 ggaagcgcac gcgacgggta cgtgacggcg gccatcagca cggcgaggcc gagcaggtcg
      481 aggctcatgc gcgcggccca gcgtcgtcgg ccgcgtcggc gggggtagcc ccgagatcga
      541 cgagctcctg catggcctcg tcgacgtcga ccgggcggcc ggtcaggtcc gaatcgccga
      601 ccacgtggcg cccgccgtcg ggaaccgcca tcgcgacgag gggccccagc aggccgcccg
      661 cgatgatccc gatctcggtg ccgaccgcca gggacgccac gaccccgatc gcggcccccg
      721 cgccggcggc gacgagctcc cgcctccccg tcacgagccc gaccgcgagg ccgcccatcg
      781 ctgccgggaa gacgacgtcg aggcccaggg tcctcgggtt ggggatcgcg tcgccgagca
      841 ggaccccggc gatcgtcgcg aggttccagg ggatgaacac cagcacgacc gccgcgatcc
      901 agtacccggg cacgtcgacc cgcccgatcc gctggaagtg cgcagcggac agggcgaagg
      961 cctcgtcggt caaaacgtgg gccatcgccg cccgctgccc cgccgacacg tggcggaccc
     1021 gtggtgcgag cgacgcgctg tacagggcgt gccgcaggtt gaggaagaac gtgaccacga
     1081 cgatcgggag ccacggcagg cccgccgcga cgtagccgac ggcgacgaac tgcgacgcgc
     1141 cggcgaacac gatcacgctc atcgccatcg cctcgaccag cgaatagccc gcgccccgcg
     1201 ccgccagccc gtacaccagc gcgaacccca ccgcggacag ggtgatgccc gccccgtcga
     1261 gcagcagccg tcgtcggccg gcacggaggt cgagcagggg agcggggtcg gtcatcgcga
     1321 cggcacggcg cgctgcgccg gggtcagcgc aggaaggtct tcgcgcaggt cccgtcgcag
     1381 acgacgaaga cggcgtgctt ctcgtgggcg tagccggcgg catgcaggag ccccggacag
     1441 cccgcggtgg ggcacttcca gacccaccag cgctcgttcg tgtcgagcgg cgtcgtctcc
     1501 gcgctgagcg tgtcggccag gccctcgcgc tccgcgacga gcgacgcgac gcgctgcgcg
     1561 cggtcggtct cggtctcgct ggtgtacggg tagttctggc cgacgttcga cgtcacgcgc
     1621 ccctccgtgt caggcgatgc cggcggcaga tcggtgtgag cgcagcctgc caccggctgc
     1681 cggaggatac catccgccga tctcgctccc gaacggcggc gggccggaaa tccggatccg
     1741 ccaccccccc gccggaccta ccggacggcc ggcagcccga agctccgcgg gtcggccgcc
     1801 gccgccagcg tcccatcgcc cgccgggccg ccgtcgatca gctcgatcgc atgctcgtgg
     1861 ccgaggctgc cgtcataggc cacgcgccgc aggtcgtgac cgcgttgcgc caggccgtcg
     1921 gcgacgcccg gtgcgaggtc gccgtccgtc aacgcgacga gcgtcggcgc ctgcattccc
     1981 gtgggctcca cgatcacgcg cggcgccgcg atggcggtcg cgatgtcagc gccgccgtcg
     2041 acgagagccg acacgagctg ggcgtggatc tggggctggt tgtcgccgcc catcgacccg
     2101 gcgacgaccc acggcctgcg ctcgccctcg cggaacagca ttcccggcag cagcgtgtgc
     2161 gcggggcgct tgccgggtgc gaggacgttc gggctcagcg gatcgagcga gaacgatgcg
     2221 ccgcggttct ggaagtgcac gccggtttcg gggtcgagca cgcccgaccc aaagcccgac
     2281 gcgttggact ggatcaggct cacggcgttg cccgcggcgt cgacgacggc gaggtagatc
     2341 gtgccgccga cgagggttcg gacgggcgtc ggcgcaggct cggctcgcga cgggtcgatc
     2401 cgggcagcaa gctcggaagc gtggtcggcg gacaggagcc gctccacggg cacgtcgtgg
     2461 aacgaggggt cggcgaggta cgcgtcgcg
//
LOCUS       DBNHXO010000280         1973 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_277128, whole genome shotgun sequence.
ACCESSION   DBNHXO010000280 DBNHXO010000000
VERSION     DBNHXO010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_277128"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="AC162A_05875"
     CDS             <1..192
                     /locus_tag="AC162A_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR5010405.1"
                     /translation="EPEFDETAAADLVAQLAAGGTLSFDPELDDEDLLPGSPRTDEPA
                     AADSPADDATTPAEDAPAD"
     gene            303..899
                     /gene="tsf"
                     /locus_tag="AC162A_05880"
     CDS             303..899
                     /gene="tsf"
                     /locus_tag="AC162A_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR5010406.1"
                     /translation="MAITAEAVRELRERTGAGMMDCKRALEETDGDIDKAVALLREHG
                     VASAAKKAGRDAREGLVGSYIHTGGRVGVLIEVNCETDFVARTDDFGKLVRDLAMQVA
                     GLAPLYVDIDAIPAEVLEAKKAALVADESVQAKPEAVREKIVDGQLKKWYREVCLLEQ
                     PFRDEDRSVRDLVTEKIATMGENIRVRRFTRYALGEEL"
     gene            959..1687
                     /gene="pyrH"
                     /locus_tag="AC162A_05885"
     CDS             959..1687
                     /gene="pyrH"
                     /locus_tag="AC162A_05885"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR5010407.1"
                     /translation="MGYQRILLKLSGEALLGERQYGVDPAFCAFIARQVAEVHATGVQ
                     IGIVVGGGNIFRGLAAAARGMDRATGDYIGMLATVMNGLALQDAIEKIGIPVRVLSAI
                     AMNEIAEPYIRRRAVRHLEKGRVTIFVAGTGNPYFTTDTAAALRAVEIGAEVLLKATK
                     VDGVYDADPMTDPSATRYSSLGYQDLLRDQLKVLDAAAVSLCLENDLPIVVFDLNAPD
                     NISRVARGEPVGTLISAERGGVTG"
     gene            1684..>1973
                     /locus_tag="AC162A_05890"
     CDS             1684..>1973
                     /locus_tag="AC162A_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043023 - ribosomal large subunit binding
                     [Evidence IEA];
                     GO_process: GO:0002184 - cytoplasmic translational
                     termination [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HIR5010408.1"
                     /translation="MTSEILAGAERKMLRAVEAMERDFQGLRTGRASTSLVERLTVDY
                     YGTQTPLNQLASISVPEAHQIVIQPWDRGVIGAIEKAIQKSDIGLTPNVDGTL"
BASE COUNT          340 a          698 c          694 g          241 t
ORIGIN      
        1 gagccggagt tcgacgagac cgccgcggcc gacctggtcg cccagctggc ggcgggcggc
       61 acgctgtcgt tcgatcccga gctcgacgac gaggacctcc tcccgggttc gccgcgaact
      121 gacgaaccgg cagccgccga ctccccggcc gacgacgcaa caacgccggc cgaggacgca
      181 cccgccgact gatccggggg cggggcggcg gcgacgtcga ccgcccgcgc ccacgaccac
      241 gaacaaccga cggcacgcgc cacctgcgca cgccgtacgc tagacacagg agcaccacgc
      301 ccatggcaat caccgccgaa gccgtcaggg agctgcgcga gcgcaccggc gcgggcatga
      361 tggactgcaa gcgcgcgctc gaggagacgg acggcgacat cgacaaggcc gtcgcgctgc
      421 tgcgtgagca cggagtcgcg tcggccgcca agaaggcggg ccgagacgcg cgcgaaggac
      481 tcgtcggcag ctacatccac acgggcggcc gggtcggcgt gctcatcgag gtcaactgcg
      541 agacggactt cgtcgcgcgc accgacgact tcggcaagct ggtccgcgac ctcgccatgc
      601 aggtcgccgg cctcgccccc ctgtacgtcg acatcgacgc catcccggcg gaggtcctcg
      661 aggcgaagaa ggccgccctc gtcgccgacg agtcggtcca ggccaagccc gaggccgtgc
      721 gcgagaagat cgtcgatggc cagctcaaga agtggtaccg cgaggtctgc ctgctcgagc
      781 agccgttccg cgacgaggac cgcagcgtgc gcgacctcgt gacggagaag atcgcgacca
      841 tgggcgagaa catccgcgtg cgccggttca cccgctacgc gctcggggag gagctgtgac
      901 cgaggacggt cgccggtccc gggccagcac ggggtcgacg accccgcgcg acggacgggt
      961 gggctaccag cggatcctgc tcaagctctc gggcgaggcg ctgctcggcg agcgccagta
     1021 cggcgtggac ccggcgttct gcgcgttcat cgcgcgccag gtggccgagg tgcacgcgac
     1081 cggcgtccag atcggaatcg tcgtcggtgg cgggaacatc ttccgcggcc tcgcggccgc
     1141 cgcgcgcggc atggaccggg cgaccggcga ctacatcggc atgctcgcga ccgtgatgaa
     1201 cggcctcgcg ctccaggacg cgatcgagaa gatcggcatc ccggtgcgag tcctgagtgc
     1261 catcgccatg aacgagatcg ccgagcccta tatccggcgg cgtgccgtgc gccacctcga
     1321 aaagggccgg gtcacgatct tcgtggccgg caccgggaac ccgtacttca cgaccgacac
     1381 agcggcggcg ctgcgagcgg tggagatcgg cgccgaggtg ctgctcaagg cgaccaaggt
     1441 cgacggggtc tacgacgcgg acccgatgac cgacccgagt gccacccgct actcgagcct
     1501 cggctaccag gacctgctgc gcgaccagct caaggtgctc gacgcggcgg ccgtgtcgct
     1561 gtgcctcgag aacgacctgc cgatcgtcgt gttcgatctc aacgcccccg acaacatcag
     1621 cagggttgcc cgcggcgagc cggtgggtac cctgatctcc gcggagcggg gaggagtcac
     1681 cggatgacca gcgagatcct ggccggcgcc gagcgcaaga tgctgcgcgc cgtcgaggcg
     1741 atggagcgcg acttccaggg cctgcggacc ggccgcgcgt cgacgtcgct cgtcgagcgt
     1801 ctgacggtcg actactacgg tacccagacg ccgctcaacc agctggcgtc gatcagcgtg
     1861 cccgaggcgc accagatcgt gatccagccc tgggaccgcg gtgtcatcgg agccatcgag
     1921 aaggcgatcc agaagagcga catcggcctg accccgaacg tcgacggcac gct
//
LOCUS       DBNHXO010000281         4530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5435465, whole genome shotgun sequence.
ACCESSION   DBNHXO010000281 DBNHXO010000000
VERSION     DBNHXO010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5435465"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..790)
                     /locus_tag="AC162A_05895"
     CDS             complement(50..790)
                     /locus_tag="AC162A_05895"
                     /inference="COORDINATES: protein motif:HMM:NF025687.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HIR5010409.1"
                     /translation="MDTNRRLAVAAGVALLIATIAQLVGVALASPILSAPDYLARVAA
                     DQDRVTLGAFFQFVGALACPAIAIALYPVLRKHGEGLALGSVGFRTIEGGLHVLIAVC
                     VLMLVTLSQAWMNATVAAAPAYEVSGGVLLAARESLGPVSVLSFGLGAIMYYWVFYRT
                     RLVPRWLSVWGLVAVPMVMLAALLVMFRVIANFSTTEVVLALPIAVQEMVLAVWLIAK
                     GFAPAAIAAEPAGETSRRPAGTSPVPVS"
     gene            complement(976..2538)
                     /locus_tag="AC162A_05900"
     CDS             complement(976..2538)
                     /locus_tag="AC162A_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010410.1"
                     /translation="MSAVLGRSASLVQPKLWWMVPAWLIGWVRPNLARATDVDALALG
                     HRNRVVVGVLLPAVAIVVALVPSIFHALMTAYPVVPEVDYLRIKLEYVYTESLLLMAA
                     AVLMGVFSPALGALFVLSFGAFDLYASSKHPLELFPLPNALLGHLIGYALLWLLAVEL
                     PIYGRLLAESAPAGGRERLRVAVAGGVATGAFVFLWTLAVPTLIRPVFTMSAGHTPTY
                     ASIAPLQVAGWSVALVAGIGATLLIWWRGGDSVLGRRRAQPGAWWRARPVRVTRHVVA
                     AALLTVGVAGLIVGPRDAVMLFLAFLLARPVASFIVTRTGLDRLTSRLPTTVRVAIAV
                     ASAFGVALLVVPRFYTNDDIDFFSVIIGLIGVLVVIEIIGADRPEPETLEAGPPDPGP
                     VEPGPIASPVVMGLLAALGLLALSALAPAVAFADNCADASDCYGLGAGAALAAAGAGA
                     LLAGRKRPPPNEDAYRQKKLDYINKQIEKHPDNPYWQSQHKYWSGDQGPQSDNKPTSN
                     QAFPSSGAATKG"
     gene            complement(2553..3233)
                     /locus_tag="AC162A_05905"
     CDS             complement(2553..3233)
                     /locus_tag="AC162A_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010411.1"
                     /translation="MGGTSIAQRLHPAYLVIGLALLANGAALVIHIVRGVPLPPLLLI
                     AWAGGLAAIAASFVRADPETRVELRKTFIVGVLAGLIATAAYDAAKALLSNLDPAPWN
                     PFEATRVFGIALLGPDAPDTVSRIAGWAFHISNGATFGMAFAFIFGGRAMAGVAWAIG
                     LGAGWGVFLEAFQLSLYPSWLGIGALDEFRQVSFLAHVVFGATLGLLVRRWLPWRGFD
                     APFRYWFP"
     gene            3382..3993
                     /locus_tag="AC162A_05910"
     CDS             3382..3993
                     /locus_tag="AC162A_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5010412.1"
                     /translation="MLDVAVIEDPAAAEVSLNPVRTRLLAELAEPASATTLATRVGMP
                     RQLVNYHLRTLEQHGLVELVEERRKGNMHERLMQATAASYVISPAALALVAPDPSRAP
                     DRLSAYWLLALAARLVRDVGALIVGGNAARKPIATFALDAEVRFATAADRAAFAEELA
                     ASVTGLVAKYHDGAAERGRPHRLIVAIHPTVTGSSATPSIKEP"
     gene            3995..>4530
                     /locus_tag="AC162A_05915"
     CDS             3995..>4530
                     /locus_tag="AC162A_05915"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5010413.1"
                     /translation="MAHPFELTKEIEIAATPEQVWEAVATGPGIDGWFLGTGNEVEPR
                     LGGTVRVSFGEESGLSTVTAWDPPRHYAHSGEGRDASGIFHAMEYTIESRAGGITLVR
                     LVHSGFLADDWEAEYDALNEGDFMYLHQMAQYVIHFLGRRASILVAMRPDVDRDAGLA
                     TLRRELGVSAGVSEGDRVR"
BASE COUNT          774 a         1592 c         1571 g          593 t
ORIGIN      
        1 ccagcgcggc ggcatggacg ccggcttcgt cgacgccgtt gtcatcgggt cagctcaccg
       61 gcacggggct cgtgccggcc ggccgccgcg acgtctcgcc ggcgggctcg gcagcgatgg
      121 cagcgggagc gaagcccttc gcgatcagcc agacggccag aaccatctcc tgcaccgcga
      181 tggggagggc cagaacgacc tccgtcgtgg agaagttggc gatcacacgg aacatcacca
      241 ggagggcggc cagcatgacc atcgggacgg ccaccagacc ccagaccgac agccaccggg
      301 gcacgagtcg cgttcgatag aacacccagt agtacatgat cgctccgaga ccgaagctca
      361 gcaccgagac ggggccaagc gactcgcggg ctgccagcaa cacgccaccc gagacctcgt
      421 acgcaggcgc ggccgcgacg gtagcgttca tccacgcttg gctcagggtg accagcatca
      481 ggacgcagac ggcgatgagg acatggagcc cgccctcgat agtcctgaag ccaaccgatc
      541 caagggccag cccttcaccg tgcttgcgca gaaccggata cagcgcgatg gcgatcgccg
      601 gacacgcaag ggcgccgacg aactggaaga acgcgccaag ggtgacccgg tcctggtccg
      661 ccgcgacgcg ggcgaggtag tcgggagcac tgaggatcgg ggacgccagc gcgacgccga
      721 ccagctgcgc gatcgttgcg atcaggagtg ccacgcctgc ggcgacggcg agccttcgat
      781 tcgtgtccat ggccttctct ctgtttctgt gggcaggcgc agcgcgccgc gcccgtcgag
      841 tcgccagtgc ggttgcgcga ccgacgattt ccccttcccc caggaccgta gcgagtcgca
      901 gctccctcag ggaagtgctg gacggcccga atcgtccacc cgtcgatcgc ggccgagtcg
      961 gcgagtccgg ggcgctcagc ccttcgttgc ggcaccggac gatgggaagg cctgattgct
     1021 cgtcggcttg ttgtcgctct ggggcccctg gtcgccgctc cagtacttgt gctggctctg
     1081 ccagtacggg ttgtccgggt gcttctcgat ctgcttgttg atgtagtcga gcttcttctg
     1141 gcgatatgcg tcctcgttcg ggggcggccg cttgcggccg gcaagcagcg caccggcgcc
     1201 ggccgccgcc agggcggcac ccgccccgag tccgtagcag tcggaggcgt ccgcgcagtt
     1261 gtcggcgaac gcgacggcgg gcgcaagcgc ggacagcgcc aggaggccga gggctgccag
     1321 caggcccatc accacgggtg acgcgatcgg accgggctcc accggtccgg ggtccggcgg
     1381 tcccgcttcc agggtctcgg gctcgggtcg gtccgccccg atgatctcga tgaccaccag
     1441 caccccgatc agtccgatga tcaccgagaa gaagtcgatg tcgtcgttcg tgtagaagcg
     1501 tggcacgacg aggagggcca ccccgaaggc cgaggccacc gctattgcca cgcggaccgt
     1561 cgtgggcagc ctcgacgtca gccggtcgag gccggtcctg gtcacgatga acgacgcgac
     1621 cgggcgggcc aggaggaacg cgaggaacag catcacggca tctcttggcc ccacgatgag
     1681 gcccgcgacg ccgacggtca ggagggccgc ggccacgaca tggcgggtga cgcgcaccgg
     1741 gcgcgcgcgc caccacgcac caggctgcgc ccgacgtcgg ccgagcacgc tgtctccgcc
     1801 ccgccaccag atcaggagcg tcgccccgat gccggcgacg agcgccaccg accagccggc
     1861 gacctggagc ggagcgatgc tggcgtacgt gggcgtgtgg cctgccgaca tcgtgaacac
     1921 gggccggatg agggtcggca cggcgagcgt ccacaaaaag acgaacgcgc cggtggcgac
     1981 gccgccggcg accgcgacgc gcagccgttc gcgccctccg gcgggggccg actccgccag
     2041 taaccgcccg tagatgggca gctccaccgc gagcagccac agcagggcgt agccgatgag
     2101 gtggcccagg agcgcgttcg ggagcgggaa cagctccagc gggtgcttcg acgaggcgta
     2161 caggtcaaac gccccgaagg acagcacgaa cagcgccccc agggccgggc tgaacacgcc
     2221 catgagcacg gccgcggcca tcaggaggag ggattccgtg tacacgtact cgagcttgat
     2281 ccgcaggtag tcgacctcgg ggacgaccgg gtatgccgtc atgagcgcat ggaagatcga
     2341 cggaacaagg gcgacgacga tcgccacggc cggcaacagc acgccgacga ccacccgatt
     2401 tcggtgaccc agtgcaagtg cgtcgacgtc ggtggcgcgc gcgaggttgg gccggaccca
     2461 cccgatgagc caggccggca ccatccacca cagcttcggc tggacgaggg atgcgcttcg
     2521 acccaacacg gcactcatga ggctgccctc catcacggaa accagtaccg gaacggcgcg
     2581 tcgaacccgc gccacgggag ccagcgccgg accagcaggc cgagcgtcgc gccgaagacg
     2641 acatgagcga ggaacgagac ctgccggaac tcgtcgagcg ctccgatgcc gagccagctt
     2701 ggatacagcg acagctggaa ggcctcgagg aacacgcccc accccgctcc gagcccgatc
     2761 gcccacgcga caccggccat cgcgcgcccg ccgaagatga acgcgaacgc catcccgaac
     2821 gtcgcgccgt tcgagatgtg gaacgcccag cccgcgatcc gggacacggt gtccggggca
     2881 tccggtccca gcagggcgat cccgaacacc cgggtcgcct cgaacgggtt ccacggggcg
     2941 gggtcaaggt tcgacaacag cgccttcgcg gcatcgtagg ccgcggtcgc gatgagcccc
     3001 gccagcacgc cgacgatgaa ggtcttccgc aactcgaccc gcgtctctgg atccgcccgc
     3061 acgaacgagg cggcgatggc ggccaggccg ccggcccagg cgatgagcag gagcggtggg
     3121 agcggaaccc cacgaacgat gtggatgacg agggcggccc cgttggcgag cagcgcgagg
     3181 ccgatgacga gatacgcggg atgaagacgc tgcgcgatgg acgtcccgcc cacgcaccct
     3241 cccgatcgtg ccggcgcgtc cccctcggcg gacgggcttc tcgcgcatcc tcccccacgg
     3301 tgggagccag cgtcaacagc ggaggtttcg gacgcaattc tctgtcttga caatgtttac
     3361 tgtcggtgac acgatgcgcg catgcttgat gtcgctgtga tcgaggaccc cgccgctgcc
     3421 gaggtctcgc tgaacccggt gcggacgcgc ctgctggccg agctggccga gcccgcctcg
     3481 gcgaccacgc tcgcgacccg tgtcgggatg cccaggcagc tggtcaacta ccacctccgc
     3541 accctcgagc agcacggcct ggtggagctc gtcgaggagc gtcgcaaggg caacatgcac
     3601 gagcggctga tgcaggcgac ggccgcctcc tacgtgatct cgcccgccgc gctcgccctg
     3661 gtcgcacctg atccgtctcg agcacccgac cggctgtccg cgtactggct gctcgccctc
     3721 gccgcccggc tcgtccgcga cgtcggggcc ctcatcgtcg gcggcaacgc cgcccgcaag
     3781 ccgatcgcga ccttcgcgct cgacgccgag gttcggttcg cgaccgccgc ggaccgcgcg
     3841 gccttcgcgg aagagctggc cgcgtcggtg accgggctgg tcgccaagta tcacgacggc
     3901 gcggccgaac gtgggcgccc acaccgcctg atcgtcgcca tccacccgac cgtgacgggc
     3961 agttccgcca cgccatcaat caaggagccc tgacatggca caccccttcg agctgaccaa
     4021 ggagatcgag atcgccgcga cgccggagca ggtctgggag gcggtcgcga ccgggcccgg
     4081 gatcgacggc tggttcctcg gcactggcaa cgaggtcgag ccgcgcctcg gcgggaccgt
     4141 ccgagtctcg ttcggcgagg agagcgggct gtcgacggtc acggcctggg atccgcctcg
     4201 gcactatgcg cacagcggcg aagggcgcga cgcgagcggc atcttccatg cgatggagta
     4261 cacgatcgag agtcgtgccg gcgggatcac attggtccgc ctcgtgcact cgggcttcct
     4321 ggccgacgac tgggaggcgg agtacgacgc cctcaacgaa ggcgacttca tgtacctcca
     4381 ccagatggcc cagtacgtca tccacttcct cggccggcgg gcgagcatcc tcgtggccat
     4441 gcgccccgat gtcgatcggg atgccgggct cgcgacgctg cggcgcgagc tcggcgtgtc
     4501 ggccggcgtc tccgagggcg accgggtgcg
//
LOCUS       DBNHXO010000282         3485 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_277577, whole genome shotgun sequence.
ACCESSION   DBNHXO010000282 DBNHXO010000000
VERSION     DBNHXO010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_277577"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..710
                     /locus_tag="AC162A_05920"
     CDS             78..710
                     /locus_tag="AC162A_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5010414.1"
                     /translation="MDETVAAPETRAERRDRATADTRATILAAARERLLAVGYANLST
                     RAVAEAAEVPLSQIHYHFGSKQQLILAVLEGENARLLERQRTMYAGSEPLWRQWDQAC
                     DFLEADLESGYVRVLQEMIAAGWSDPEVASAVREFVGGWYRLLAEVAERVSGTRGGLG
                     PFTPTEVAALMGLPFLGAESIILLGFTETELPARSALRKLGDVIRVMEEA"
     gene            735..1136
                     /locus_tag="AC162A_05925"
     CDS             735..1136
                     /locus_tag="AC162A_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5010415.1"
                     /translation="MTVDRAAAHAAVAADDEAAIRATIDDYYLGWFDADGARMARALH
                     PELAKRGWLRGRDGASLLEPDTFDTMVGFAADGHGRQTDPATRRYDVQVNDVDGDIAA
                     AVVHAVPYVDYLHLVRTPDGWRIVNALWCPA"
     gene            1133..2101
                     /locus_tag="AC162A_05930"
     CDS             1133..2101
                     /locus_tag="AC162A_05930"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5010416.1"
                     /translation="MSVAPPEEAVSAQRREPSRARYPDESGHVERHGVRVFWERYGDG
                     EPAILLMPTWSVVHSRHWKSQTAFLARFFRVITFDGRGNGRSDRPQGSAAYAGVEFAD
                     DAAAVLDATGTGSVVACGLSMGAGYAARLAIEHPDRVRGIVMFGESIPFDGASNDAGE
                     GGPDTQFDDPQPDDEGWNKYNAHYWRRDWRGFAEWFAGDRIFSEAHSTKPIEDFVGWF
                     LETDPETIIAAEREPYMRPTADWEPGPPRPGRAARYLQQVSCPVLMVHGTEDRISSIA
                     HARRLAALLGARLVEIEGGGHAPIARDPVLANLIIRDFVRGLEATR"
     gene            2098..3024
                     /locus_tag="AC162A_05935"
     CDS             2098..3024
                     /locus_tag="AC162A_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5010417.1"
                     /translation="MTAPTIDWRRTPNSGPARLPERSGTATTDDGVRLAFDVYGRGSP
                     TIVLLPSNPIIHSRQWKAQIHYLSRYYRVVTYDGRGNGLSDRPTDPGAYSDRRLVEDI
                     EVVLDATDTPDAVLVGLCGDGVWRAIRFAATKPDRVLGVVAFAVGVPRISPPHPWHVE
                     WSFEDELPTDEGWAKTNVHYWRRDYADFARFFFAEVACEPHSTKIVEDAVTWALDGSV
                     DAMVADMRADFDLDLDGVEAVCRSLTAPMVIVHGTEDRCQPVARAHRLAELTGAPLVV
                     VEGADHMIPGRHPVLANLVIRRFVESLKEART"
     gene            3021..>3485
                     /locus_tag="AC162A_05940"
     CDS             3021..>3485
                     /locus_tag="AC162A_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5010418.1"
                     /translation="MTAATTTWTRSLARKRRALYISSPIGLGHAQRDVAIADELRRLH
                     PDLEIDWLAQHPVTAVLEARDERIHPLSAELASESAHITSESSDHDLNAFQAIRRMDE
                     ILVANFMVFHDAVEQGEYDLVIGDEAWDVDHFLHENPELKRTAFAWMTDFVGW"
BASE COUNT          501 a         1239 c         1229 g          516 t
ORIGIN      
        1 ctcggctacg ggctgctgtt cgcggccgtc tactccgtcg cgtccgtcgc ctgatggggt
       61 cgtatgctcg tgacgacgtg gacgagaccg ttgccgcacc cgagacgcgg gccgagcgca
      121 gggaccgggc gacggccgat acccgggcca ccatcctggc ggccgcccgg gaacggctgc
      181 tggcggtcgg gtacgcgaac ctgtcgaccc gggccgtggc ggaggccgcc gaggtcccgc
      241 tcagccagat ccactaccac ttcggcagca agcagcagct catcctggca gtcctcgagg
      301 gcgagaacgc ccggctcctc gagcgccagc ggacgatgta tgcgggatcc gagccgctgt
      361 ggcgccagtg ggaccaggcg tgcgacttcc tcgaggcgga cctcgagtcg ggctacgtcc
      421 gcgtgctcca ggagatgatc gctgccggct ggtcggaccc cgaggtcgcg agcgcggtgc
      481 gcgagttcgt gggtggctgg taccggctcc tcgcggaggt tgcggagcgc gtttcgggca
      541 cccgcggtgg cttgggtccg ttcacaccga ccgaggtcgc ggccctgatg ggcctcccct
      601 tcctcggcgc ggagtcgatc atcctgctcg ggttcaccga gacggagctt cccgcgcgct
      661 cggccctgcg caagctcggc gacgtgatcc gcgtgatgga ggaggcgtga cgtgcgacga
      721 cacgagatct cccggtgacg gttgaccgcg ccgccgccca cgcggcagtg gcggccgacg
      781 acgaggccgc gatccgggcg acgatcgacg actactacct cggctggttc gacgcggacg
      841 gcgctcgcat ggccagggcg ctgcatccgg agctggccaa gcgtggctgg ctgcgcgggc
      901 gcgacggtgc ctcgctcctc gaacccgaca cctttgacac gatggtcgga ttcgctgccg
      961 acggccatgg ccggcagacc gatcccgcca cgcgccgcta cgacgtccag gtcaacgacg
     1021 tcgacggcga catcgccgcg gccgtcgtgc acgccgtgcc gtacgtggac tacctccacc
     1081 tcgtgcggac gcccgacggc tggcggatcg tcaacgcgtt atggtgcccg gcgtgagcgt
     1141 cgcgccaccc gaggaggccg tctcggcgca gcgtcgagag ccgtcgcgcg cccgctaccc
     1201 ggacgagagc ggccatgtcg agcgccacgg cgtccgggtg ttctgggagc gctatgggga
     1261 cggcgagccg gccatcctcc tgatgccgac ctggtcggtg gtccactccc gccactggaa
     1321 gagccagacc gccttcctcg cgcgcttctt ccgcgtgatc acgttcgacg ggcgagggaa
     1381 cgggcggtcg gaccgaccgc aaggctccgc ggcgtacgcc ggcgtggagt tcgccgacga
     1441 cgcggcggcg gtcctggacg cgacgggcac gggcagcgtc gtcgcctgcg gcctgtcgat
     1501 gggcgcgggc tatgcggcca ggctggcgat cgagcatccc gatcgcgtgc gcgggatcgt
     1561 gatgtttggg gaatcgatcc cgttcgacgg tgcgtccaac gacgccggcg agggcgggcc
     1621 tgacacccag ttcgacgatc cgcagcccga cgacgagggc tggaacaagt acaacgccca
     1681 ctactggcga cgcgactggc gcggcttcgc cgagtggttc gcgggcgacc gcatcttctc
     1741 cgaggcgcat tccacgaagc ccatcgagga cttcgtcggc tggtttctgg agaccgatcc
     1801 ggagacgatc atcgccgccg agcgcgagcc gtacatgcgc cccaccgccg actgggagcc
     1861 cggcccgccg aggccggggc gcgcggcgcg ctacctgcag caggtcagct gccccgtcct
     1921 gatggtccac gggacggagg accggatctc gtccatcgcc catgcccggc ggcttgcggc
     1981 gctgctcggc gcgaggctgg tcgagatcga gggtggtggc catgccccga tcgcccgcga
     2041 cccggtgctg gccaacctga tcatccgcga cttcgtccgc ggcctggagg cgacgcggtg
     2101 acggcgccca ccatcgactg gcgtcggacg ccgaacagcg ggccggcgag gctcccggag
     2161 cgctcgggca ccgcgacgac cgacgacggc gttcgactcg cgttcgacgt gtacgggagg
     2221 ggttcgccga cgatcgtgct cctgccgtcg aatccgatca tccactcgcg ccagtggaag
     2281 gcccagatcc actacctcag ccgctactac cgcgtggtca cctacgacgg gcgaggcaac
     2341 ggcctctcgg accgccccac agaccccggc gcctactcgg accggcgcct ggtcgaggac
     2401 atcgaggtgg tcctcgacgc gaccgacacc ccggacgcgg tcctcgtcgg gctgtgcggc
     2461 gacggcgtct ggcgcgcgat ccggttcgca gccacgaagc ccgaccgggt gctgggtgtc
     2521 gtcgcgttcg cggtcggggt gccccggatc tcgccgccgc acccttggca cgtcgagtgg
     2581 tcgttcgagg acgagttgcc gaccgacgag ggttgggcca agaccaacgt gcactactgg
     2641 cggcgtgact acgcggactt cgcgcgattc ttctttgccg aggttgcctg cgagccgcac
     2701 tcgaccaaga tcgtcgagga cgcggtgacc tgggccctcg acgggtcggt cgacgcgatg
     2761 gtcgcggaca tgcgcgccga cttcgatctc gacctcgacg gcgtcgaggc cgtctgccgg
     2821 tcgctcaccg ctccgatggt catcgtccac gggacggagg accgctgcca gccggtcgcc
     2881 cgtgcccacc gcctcgccga gctgaccggc gccccgctgg tggtcgtcga gggcgccgat
     2941 cacatgatcc ccggccgcca tcccgtgctc gccaacctgg tcatccgtcg attcgtcgaa
     3001 tcgctgaagg aggcgcggac atgaccgcgg ccacgaccac atggacccgc tccctcgcgc
     3061 gaaagcggcg cgccctctac atctcgtccc cgatcgggct ggggcacgcc cagcgggacg
     3121 tcgcgatcgc cgacgagctg cgcaggctcc acccggacct cgagatcgac tggctcgccc
     3181 agcacccggt cacggcggtg ctcgaggcgc gtgacgagcg gatccacccg ctcagcgcgg
     3241 agctcgcctc ggagtcggcg cacatcacca gcgagtcgtc ggaccacgac ctcaacgcct
     3301 ttcaggcgat ccggcggatg gacgagatcc tggtcgccaa cttcatggtg ttccacgacg
     3361 ccgtcgagca gggcgagtac gacctcgtga tcggcgacga ggcatgggac gtcgatcact
     3421 tcctgcacga gaaccccgag ctcaagcgga cggcgttcgc ctggatgacc gacttcgtcg
     3481 gctgg
//
LOCUS       DBNHXO010000283         2256 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1071222, whole genome shotgun sequence.
ACCESSION   DBNHXO010000283 DBNHXO010000000
VERSION     DBNHXO010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1071222"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="AC162A_05945"
     CDS             complement(<1..110)
                     /locus_tag="AC162A_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HIR5010419.1"
                     /translation="MRQPDFSRRVVVTGLGVISPVGNDKATAWNNLVNGNS"
     gene            complement(144..1136)
                     /gene="fabD"
                     /locus_tag="AC162A_05950"
     CDS             complement(144..1136)
                     /gene="fabD"
                     /locus_tag="AC162A_05950"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007800263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR5010420.1"
                     /translation="MSQIAFVFPGQGSQSVGMGQALAHASPAAAAVFEAADAALGEPI
                     SRLAWDGPEDDLNRTENAQPALLAASIAYLEAVRERWAELDIDIPEPAFAAGHSMGQY
                     SALVAAGALDLADAVRLVRRRGELMQASGAGREGRMAAIIGLDDARIPELVAQASAHG
                     VFGVANRNSPGQVVISGERPAVEASLAIAKELGAKRAIELPVSVAAHSPLMAEAAEGM
                     RQTLAGISFRDPEPPLLANADARPITTADGCRAELVEHLTTGVDWVGAIEAMQAKGVT
                     TFIEVGPGRVLTGLIKRIAPDALPLALDDPAAADRLAIPFAESAAVEAAFESAV"
     gene            1240..>2256
                     /locus_tag="AC162A_05955"
     CDS             1240..>2256
                     /locus_tag="AC162A_05955"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR5010421.1"
                     /translation="MTDVEQPAPAQPVKPRRPRPSDPAAGPELPEALVRAPRRGPAQS
                     RVIARMFEADLSAPVVAAGTRIQNVRQISERVAMGRALWRELVVGFASQLGELNLGFT
                     QLAALYVLAEGSTTTVADLADAIGRSPSATSRLVDGLVKRRLVERRREHDDGRLRTVW
                     LTQRGKAVLRMVDRARAEQFLATVRPMPRGERALIAMGVAALATHAISRRGRSSAAGA
                     ELRVPHGGTQLAHNRGASHHDEDADGPAPLLRGAVPDRAPSPRWKLGRDGRGSPAARS
                     SRHRRRAVVGSAQPVPLQDVRRERDRRAGRRERSAAVARDHVVQDGQAPAQRVDADPL
                     VHRVE"
BASE COUNT          322 a          778 c          859 g          297 t
ORIGIN      
        1 gagttgccgt tgacgaggtt gttccaggcg gtggccttgt cgttgcccac cgggctgatg
       61 acgccgaggc cggtgacgac gacgcggcgc gagaaatcag gttggcgcaa ggtgatcctt
      121 tcggggggtg acggtcgccg ccgtcagacg gcggactcga aggcggcctc gacggccgcg
      181 gattcggcga acgggatggc caggcgatcc gccgcggcgg ggtcgtcgag cgcgagcggg
      241 agcgcgtctg gcgcgatccg cttgatcagg ccggtcagca cccgtcccgg cccgacctcg
      301 atgaaggtgg tgacgccctt ggcctgcatg gcctcgatcg cgccgaccca gtcgacgccg
      361 gtcgtcaggt gctcgacgag ctcggcgcgg cagccgtcgg ccgtggtgat cggccgggcg
      421 tcggcgttgg cgaggagcgg tggctcagga tcgcggaacg agatcccggc caatgtctgg
      481 cgcatgccct cggcggcctc agccatcagc ggggagtggg cggccaccga cacgggcagc
      541 tcgatggcgc gcttggcgcc gagctccttc gcgatggcca gcgacgcctc gaccgccggc
      601 cgctccccgg agatcacgac ctggcccggc gagttccggt tcgcgacgcc gaacaccccg
      661 tgggccgagg cctgcgcgac caactcgggg atccgggcgt cgtccaaccc gatgatcgcg
      721 gccatccggc cctcgcgccc ggcaccggac gcctgcatca gctcgccgcg gcggcggacc
      781 aggcggacgg cgtcggcgag gtcgagggct cctgcggcca cgagggccga atactggccc
      841 atcgaatggc cggcggcgaa ggcgggctcg gggatgtcga tgtcgagctc ggcccagcgc
      901 tcgcgaacgg cctcgagata ggcgatcgac gcggcgagga gtgccggctg ggcgttctcg
      961 gtgcggttga ggtcgtcctc ggggccgtcc caggcgaggc ggctgatcgg ctcaccgagg
     1021 gccgcgtcgg cggcctcgaa gacggctgcc gcggcggggg atgcatgggc gagggcctgg
     1081 cccatgccga cggactggct tccctggccg ggaaacacga aagcgatctg actcatctgg
     1141 cccaagggta ctggcgaacg cccggcggac cgggcgaatc catgcacggt tgcaggaatc
     1201 ggcaggcggt tttacgctca atcgggtgag gatctgcccg tgaccgacgt cgaacagccc
     1261 gctcctgccc aacccgtcaa gccccgccgg ccccgtccgt ccgacccggc agccggaccc
     1321 gagcttcccg aagcgctcgt gcgagcgcct cggcgcgggc ctgcccagag ccgggtcatc
     1381 gcccggatgt tcgaggccga cctctcggcg ccagtggtcg ccgccggcac gcggatccag
     1441 aacgtgcgcc agatctcgga gcgcgtcgcc atgggccgcg cgctgtggcg ggagctggtc
     1501 gtcggcttcg cgtcccagct cggcgagctc aacctcgggt tcacgcagct cgcggcgctg
     1561 tacgtgctcg cggaggggtc gacgaccacg gtcgcggacc tcgcggacgc gatcgggcgc
     1621 tcgccgtcgg cgacctcgcg gctggtcgac ggcctggtaa agcggcgcct ggtcgagcgt
     1681 cggcgcgagc acgacgacgg gcgccttcgc accgtgtggc tcacccagcg gggcaaggcc
     1741 gtcctgcgga tggtcgaccg ggcgcgggcg gagcagttcc tcgcgacggt ccgcccgatg
     1801 ccgcgcgggg agcgggccct gatcgcgatg ggggtcgcgg ccctcgcgac ccacgccatc
     1861 agccggcgcg gccggtcgtc cgcggcggga gctgagctcc gggtgccaca cggcgggacg
     1921 cagctggcgc acaatcgagg cgcaagccac catgacgagg acgccgatgg accagctccg
     1981 ctcctacgag gtgcagtacc ggatcgagca ccgtcaccac gatggaagct gggccgagat
     2041 ggtcgaggat cgccagccgc acgatccagc cgacatcgac gtcgagcggt cgtggggagt
     2101 gcgcagcctg ttccgctgca ggacgtgcga agagagcgtg atcgtcgagc cgggcgacgg
     2161 gagcgttcag ccgcagtagc ccgcgaccac gtcgtacagg acgggcaggc cccagcgcag
     2221 cgcgtcgacg ccgacccgct cgtccaccgc gtggaa
//
LOCUS       DBNHXO010000284         1926 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3053470, whole genome shotgun sequence.
ACCESSION   DBNHXO010000284 DBNHXO010000000
VERSION     DBNHXO010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3053470"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="AC162A_05960"
     CDS             <1..875
                     /locus_tag="AC162A_05960"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HIR5010422.1"
                     /translation="LEVEVMDQAELTRLGCGGLLGVNGGSADEARMIKLTYRPTAGPE
                     QATAHLALVAKGIMYDSGGISLKPSDAVHATMKNDMSGAAAVLAAMSVLGDLGCRNTV
                     TGYLSCTDNMPSGTALKLGDVITMYGGTTVEVMNTDAEGRLVMADALVMAAGLEPDAI
                     VDIATLTGATMRALGTWIAGLMGNDQALMDQIGAASERTDERTWPFPLERRYRRELDS
                     DVADIKNMGGPNAGQITAALFLDEFTGGHPWAHIDIAGVAQADPPESWRTKGCTGFGA
                     RLLVELALDFTPPA"
     gene            899..>1926
                     /locus_tag="AC162A_05965"
     CDS             899..>1926
                     /locus_tag="AC162A_05965"
                     /inference="COORDINATES: protein motif:HMM:NF015746.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HIR5010423.1"
                     /translation="MTAAVAVEGGGNKGFTAGLLDGIERLGNKVPHPAIMFFYLILFV
                     IVLSHVLFVLGVSVTEDIAVPVPIAVDPNYYEDTVQPGVYGPGMTDLYGDDFEIKTQT
                     IAIQSLLTVEGIRFIFTSFVANFAGFGVVAVTFIAMMGAGVAEGAGLMNALIRKLVAV
                     SPRGMLSFILIMVGVLSSVATDAGYLILIPLGAAAFASVGRHPLAGMAACFAGVGSIF
                     GVNLILSPSDAMITEITNEAIGLIPGQAPITIVSNWFFAIACSIILAIVAAVLTERVI
                     EPRLGPWKPAGDHVEEDHTPEAASKESRGLKFAGIAFLISIVVVVLLTAPSGAPLRDP
                     LTGDIIGAT"
BASE COUNT          293 a          670 c          660 g          303 t
ORIGIN      
        1 gcctcgaggt ggaggtcatg gaccaggcgg agctgacccg gctcggctgc ggtggcctgc
       61 tcggcgtcaa cgggggcagc gccgacgagg cgcgcatgat caagctcacc taccgcccga
      121 cggcgggacc cgagcaggcg acggcgcacc tcgcgctggt cgccaagggg atcatgtacg
      181 actcgggcgg gatcagcctc aagccatccg atgccgtgca cgccacgatg aagaacgaca
      241 tgtcgggggc cgcggcggtc ctcgcggcga tgtcggtgct gggcgacctc ggctgccgga
      301 ataccgtgac gggctacctc agctgcaccg acaacatgcc ctcgggtacg gcgctcaagc
      361 tgggcgatgt gatcacgatg tacggcggca cgaccgtcga ggtcatgaac accgacgccg
      421 agggccgcct ggtgatggcc gacgcgctgg tcatggccgc cggtctcgag cccgacgcca
      481 tcgtcgacat cgcgaccctg accggcgcga cgatgcgcgc cctgggcacc tggatcgccg
      541 gcctcatggg caatgaccag gccctgatgg accagatcgg ggcggcgtcc gagcggaccg
      601 acgagcggac ctggccgttc ccgctcgagc ggcgctaccg gcgcgagctc gactccgacg
      661 tcgcggacat caagaacatg ggcggtccga acgccgggca gatcaccgcc gccctgttcc
      721 tcgacgaatt caccggcggc cacccctggg cgcacatcga catcgccggg gtggcgcagg
      781 ccgatccgcc cgagtcgtgg cggaccaagg gctgcaccgg gttcggggcg cgcctgctcg
      841 tggagctcgc gctcgacttc acgccaccgg cctgatcgct acgacggagg aggaacggat
      901 gacggcagcg gtcgcggtcg aaggcggggg caacaagggg ttcaccgccg ggctgctcga
      961 cggcatcgag cggcttggga acaaggtccc gcacccggcg atcatgttct tctacctgat
     1021 cctgttcgtg atcgtgctgt cgcacgtcct gttcgtgctc ggcgtgagcg tgaccgagga
     1081 catcgcggtc ccggtgccca tcgccgtcga cccgaactac tacgaggaca ccgtccaacc
     1141 gggcgtctac gggccgggca tgacggatct gtacggcgac gacttcgaga tcaagacgca
     1201 gacgatcgcc atccagagcc tgctgaccgt cgagggcatc cggttcatct tcacgtcgtt
     1261 cgtggccaac ttcgccgggt tcggcgtcgt cgccgtcacg ttcatcgcga tgatgggtgc
     1321 gggcgtcgcc gagggcgcgg ggctcatgaa cgcgctcatc cgcaagctgg tcgcggtctc
     1381 gccgcgcggg atgctgtcgt tcatcctgat catggtcggc gtgctctcga gcgtcgcgac
     1441 cgacgccggc tacctgatcc tgatcccgct cggggcggcg gcgttcgcga gcgtgggcag
     1501 acatccgctc gccggcatgg ccgcctgctt cgccggtgtc gggtcgatct tcggggtcaa
     1561 cctcatcctg tcgccgtccg acgcgatgat caccgagatc accaatgagg ccatcggcct
     1621 cattcccggc caggcgccga tcaccatcgt gtccaactgg ttcttcgcga tcgcctgctc
     1681 gatcatcctc gccatcgtcg cggccgtgct gaccgagcgg gtcatcgagc cccggctcgg
     1741 cccctggaag ccggccggcg accacgtcga ggaggaccac accccggagg cggcgtcgaa
     1801 ggagtcgcgc ggcctgaagt tcgccgggat cgcgttcctc atctcgatcg tcgtggtcgt
     1861 cctgctgacc gccccgtccg gcgcgcccct gcgcgacccg ctgaccggcg acatcatcgg
     1921 cgcgac
//
LOCUS       DBNHXO010000285         2124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2656689, whole genome shotgun sequence.
ACCESSION   DBNHXO010000285 DBNHXO010000000
VERSION     DBNHXO010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2656689"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="AC162A_05970"
     CDS             complement(<1..728)
                     /locus_tag="AC162A_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HIR5010424.1"
                     /translation="MFVVMRTGATEAEVIGVKGLILAEGLTPFENAGEARTAIAVVGE
                     IGRGRDELTARLQALPGVERVTAISKPYKLVSREFHPENTVIRALDAVIGDGSLTVMA
                     GPCSIESRDQLLETVDHVATHGATVIRGGAFKPRTSPYSFQGLGVEGLRYLAEARERT
                     GLPVITEVMEPSQVDIVSEYADILQIGTRNMQNFSLLLACGRAGRPVMLKRGFGATIE
                     EWLMAAEYIASAGNPNIIMCERGIR"
     gene            1265..1356
                     /locus_tag="AC162A_05975"
     tRNA            1265..1356
                     /locus_tag="AC162A_05975"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1299..1301,aa:Ser,seq:gct)
     gene            1364..1439
                     /locus_tag="AC162A_05980"
     tRNA            1364..1439
                     /locus_tag="AC162A_05980"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1397..1399,aa:Arg,seq:gcg)
     gene            1821..>2124
                     /locus_tag="AC162A_05985"
     CDS             1821..>2124
                     /locus_tag="AC162A_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020042826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HIR5010425.1"
                     /translation="MKRSILLAILPLALVVAACSSTASATPAPATATPPAATPAASPS
                     TAAMGDIVATAKAAGTFSTLLTAATAAGLVETLQGAGPFTVFAPTDDAFAALPAGTL"
BASE COUNT          320 a          774 c          695 g          335 t
ORIGIN      
        1 cggatgccgc gctcgcacat gatgatgttc gggttgccgg ccgacgcgat gtactcggcg
       61 gccatcagcc actcttcgat cgtcgccccg aacccgcgct tgagcatcac gggccggccc
      121 gcgcgcccgc aggcgagcag cagcgagaag ttctgcatgt tgcgggtgcc gatctggagg
      181 atgtcggcgt attcggagac gatgtcgacc tggctgggct ccatgacctc ggtgatcacc
      241 ggcagtcccg tccgctcgcg cgcctcggcg aggtagcgca gcccctcgac gcccaggccc
      301 tggaagctgt acgggctggt gcggggcttg aacgccccgc cgcggatcac ggtcgcgccg
      361 tgcgtggcga cgtggtcgac ggtctcgagg agctggtcgc ggctctcgat cgagcacggg
      421 ccggccatca ccgtcagcga gccgtcgccg atcaccgcgt cgagcgcgcg gatgacggtg
      481 ttctcggggt ggaactcgcg cgagaccagc ttgtagggct tcgagatcgc cgtcacccgc
      541 tcgacgcccg gcagcgcctg gaggcgcgcc gtcagctcgt cgcggccgcg cccgatctcg
      601 cccacgacgg cgatcgccgt ccgggcctcg cccgcattct cgaacggggt cagcccctcg
      661 gccaggatca ggcccttcac gccgatgacc tcggcctcgg tggctccggt ccgcatcacc
      721 acgaacatct ggtcagcgct tcctctggaa accgtcctct gagaacaaaa aagcagaggt
      781 caatgacctc tgctcacccg gcacgacccg caatcccggt cgtcagtctc cggggtcggt
      841 catgccgggc aggtaaagcg atagatggcg cacatcggtg ggtggacggt agcccttgcg
      901 agggtcccgc gtcaacttcg gccgagcgtg gcgcgtgcgt ggcgcggacc agcgcgcgcc
      961 cgccgaccct gtgtgcgccg gctgcacatt cctgcgcgtc gtctggcgac tccgggtctg
     1021 atccatccag atctgtgtac ccaccgcacg ccgccgttat cgcggcctcc tcgggcggcc
     1081 caacctgcac gatgcgtgcg cccggcgcac aaatggaacg cccaacggcg ttgtcacggc
     1141 aggaatgtga gcccggtgca caggctgccc gatgttgtgc tcgagccaca cgaaatcgcc
     1201 cgagtggacg ccctcggcct atctggaccc gatcgggccg gatgctagaa ttcgcgccgc
     1261 tgctggagag gtcgcatagc ggtctagtgc agcggtttgc taaaccgccg agtggggtaa
     1321 cctgctccga gggttcgaat ccctccctct ccgccacgct gttgcgcccg tagctcagtg
     1381 gatagagcac cagcttgcgg agctgggggt cgtgggttcg aatcccgccg ggcgcgccat
     1441 tcccgaaggt gataaggggt ccgcgacgcg gatcggacgt cgagcgccct acgctccgcc
     1501 ccttcaggaa gacgcggctg gactcaaacc cgaagggcgc gaccggagcg gcccagtcgc
     1561 agaacgctgc cgcggccagg gcccggtctc gacctccatg cgctcgacct tctcgagcag
     1621 cgtggcccaa gcgcttagcc ccggacccct gcctgaacgt gaccctttcc gatggctcgt
     1681 gcgaccagcc acgggctccg ttggcgggcg cgacttgaca ggaaagattt agtgctaaat
     1741 actgcggcgg caaccggagc acccgccccc ggtccttagc actggcagcg tccctgcccc
     1801 ggaaaggaac cctcatcgac atgaagcgtt caatcctcct cgcgatcctg ccgctcgccc
     1861 tcgtagtggc ggcgtgcagc agcaccgctt cggcgacgcc ggccccggcc acggccaccc
     1921 cgccggcggc cacgcctgcc gcgtcgccca gcacggccgc gatgggcgac atcgtcgcca
     1981 ctgccaaggc cgccggaacc ttcagcaccc tgctgacggc tgccaccgcc gcgggtctcg
     2041 tcgagaccct ccagggcgcc ggcccgttca ccgtctttgc gccgacggac gacgcgttcg
     2101 ccgccctccc cgccggcacg ctgg
//
LOCUS       DBNHXO010000286         5490 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3252679, whole genome shotgun sequence.
ACCESSION   DBNHXO010000286 DBNHXO010000000
VERSION     DBNHXO010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3252679"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /gene="dnaJ"
                     /locus_tag="AC162A_05990"
     CDS             <1..995
                     /gene="dnaJ"
                     /locus_tag="AC162A_05990"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR5010426.1"
                     /translation="QERFKEINEAYQVLSDPSRRQAYDMFGKAGVSGAGADGGFGNAG
                     FGGFADIFDAFFGGTAGGASRRAARPQTGSDLRYDMRITFQEAVLGTEKEIEFPVLGR
                     CETCEGTGAKPGTTPSQCPQCNGRGEIRSVRQTMLGQMVNVSACPRCHGEGKIVESPC
                     ETCRGEGRTERKRTLRVAIPAGIDEGHQIRLSNEGEVGPRGGAPGSLYVAVHVTDHPS
                     LTREGTELYYEADLSIAQAALGTKIQVPTVEGEDTEVEIKPGTQPGTEIRLRGKGVPH
                     LRRQSVRGDLHVIANVVVPTKLTKRQRELLVQYAADGGEAVAQHAGLREKLGLG"
     gene            1036..1986
                     /gene="prmA"
                     /locus_tag="AC162A_05995"
     CDS             1036..1986
                     /gene="prmA"
                     /locus_tag="AC162A_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR5010427.1"
                     /translation="MDLAAAAATPGTWLELSIAADHEAVEAVSEILSRAAPGGTSVEP
                     AYELVDEGLAARVDLARPALVRAHLPVLDVAAVRAAVERVERDLGHLQAFGLRPIGDL
                     EASLVLEADWANAWKQHFPVLRIGRRIVIRPTWRRHRREPGDVVIALDPGMAFGTGLH
                     PTTRLCLAALESMADRDVPMAEVLDVGSGSGILSIAAARLGAGDVLALDIDEMAVDAT
                     RANAKRNGLVRRVRARQGSSPSGEGPFDIVLANLIASVLLALADGLIGDTRPGGTLLA
                     SGIFENRETEVAAAFTSRGLSIANRWAEGDWVALEVRRPA"
     gene            2042..2530
                     /locus_tag="AC162A_06000"
     CDS             2042..2530
                     /locus_tag="AC162A_06000"
                     /inference="COORDINATES: protein motif:HMM:NF021538.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HIR5010428.1"
                     /translation="MPDLSSLFPAILATHITLAICLLVPAVLLPFTLRTRSVAPNATP
                     AQPGRVVRGLSAVQAHGTVVIGAGLALTGMAMIVVLGPRMLEQPWLLVSLATYAVAAI
                     VAFVIQRPQLRKLQARDGIATDADRAAWRDRARRQRYVAYGLTSMVGLIAFLMSTKPQ
                     LW"
     gene            2530..3978
                     /gene="mtaB"
                     /locus_tag="AC162A_06005"
     CDS             2530..3978
                     /gene="mtaB"
                     /locus_tag="AC162A_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035598 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HIR5010429.1"
                     /translation="MPRSAAIVNLGCKVNQSEMEGAARLLREAGVPLVDPDRAADLYL
                     VNTCTVTATADEKSRAAVRRARRANPRAEIVVTGCSVQVGPEAFARVDPDARLVGNDG
                     KAGYLAELEGLLGAAMRPNEPLPTLSGVDLVAAPIDGIADDRAAVERTRAFVKVQDGC
                     SFYCTYCIIPRARGGERSLHPETVLADVRRALSAGHREIVLTGINIGTYDGGWSERGH
                     RGTHSRSALTLAGLVRRILAETDVERIRLSSIEPQHVDDELLEAWVVGAPRTLPHLHL
                     PLQSGDDGVLRRMGRRYLTADYLRVVARAREAIPGVAVHADVIAGFPSEDDAAFARTL
                     AFARQIEFAGLHVFRYSARPGTPATRMAGQVDERTKKARAGDLLAVAADARAAFARQG
                     LGTVTRVLAERRLPDGRWVGHAGDYVSTAVTTRQGDRGDLENAILTVRRTGIDGDAAD
                     RATGEILAIDPPRRALRPAIPVIALGATAHAG"
     gene            3968..4312
                     /locus_tag="AC162A_06010"
     CDS             3968..4312
                     /locus_tag="AC162A_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR5010430.1"
                     /translation="MPDDCLFCRIAAGEIPATKLHEDADVIAIRDINPQAPTHVLVMP
                     VAHIVSAADLTDADGPLLGRLFSIAAEIARSEGLDGGWRLVANVGRDGGQSVDHLHLH
                     VLGGRPMTWPPG"
     gene            4309..4881
                     /locus_tag="AC162A_06015"
     CDS             4309..4881
                     /locus_tag="AC162A_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010431.1"
                     /translation="MTAPAANEERRGGPPAWFAIIGIGGLLLGVVLIAAQVLGIGVGG
                     TAPAATIAPTGEAAQYTHDLVTKTLTDASFQVIDPLTPYRPGESPALIGVPRRSVQAV
                     LPSDPDKGYIVIYELPTNGEADRLGKDFAAYLGSGTGAVQYPRDAQFVLRRVGPTLVF
                     FSWSPLVSPDPEMARLASTLATIGEPVTAP"
     gene            complement(4887..>5490)
                     /locus_tag="AC162A_06020"
     CDS             complement(4887..>5490)
                     /locus_tag="AC162A_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase family protein"
                     /protein_id="HIR5010432.1"
                     /translation="PLGCVGWPPKPLEMRIVEDGEIQFRGPSLTPGYWNDPEATKAAF
                     TDDGWYMSGDLGAFDKEGRLHLHGRKKDIIVLPNGFNVFPEDLENALRVAGIRDSVAI
                     ETKPGRIEAIVLAPGTHGVPGDPRATPAIESASPEDVRRQIDAAVKAANGSLGPNQRI
                     AGWRVWPEADVPRTHTFKVKRDRVRAWAEISESLPVAEEA"
BASE COUNT          760 a         2023 c         1944 g          763 t
ORIGIN      
        1 cgcaggagcg gttcaaggag atcaacgagg cctaccaggt cctctccgat cccagccgcc
       61 ggcaggccta cgacatgttc ggcaaggcag gggtctcggg cgccggcgcc gacgggggct
      121 tcggcaacgc cggcttcggc gggttcgcgg acatcttcga cgcgttcttc ggcggcaccg
      181 cgggcggagc ctcccgacgc gcggcccggc cgcagaccgg atccgacctc cgctatgaca
      241 tgcggatcac gttccaggag gccgtcctcg gcaccgagaa ggagatcgag ttccccgtcc
      301 tcggccggtg cgagacgtgc gagggcaccg gggccaagcc cggcaccacg ccgtcccaat
      361 gcccgcagtg caacggccgc ggcgagatcc gctcggtccg ccagacgatg ctcggccaga
      421 tggtcaacgt ctcggcgtgc ccgaggtgcc acggcgaggg caagatcgtc gagtcaccgt
      481 gcgagacctg ccgaggcgag ggccggacgg agcgcaagcg gacgctgcgg gtggcgatcc
      541 ccgcgggcat cgacgagggc caccagatcc gcctgtccaa cgagggcgag gtcgggccgc
      601 gcggcggcgc accgggcagc ctctacgtcg ccgtgcacgt gacggaccat ccgtcactca
      661 cccgtgaagg caccgagctc tactacgagg ccgacctctc gatcgcccag gccgcgctcg
      721 ggacgaagat ccaggtgccg acggtcgagg gcgaggacac agaggtcgag atcaagcccg
      781 ggacgcagcc gggcaccgag atccggctcc gcggcaaggg cgtaccgcac ctccggcgcc
      841 agtcggtccg cggcgacctc cacgtgatcg ccaacgtcgt cgtcccgacg aagctcacga
      901 agcgccagcg cgagttgctc gtgcaatacg cggccgacgg cggcgaggcc gtcgcgcagc
      961 acgccggcct gcgggagaag ttgggccttg gctagcggcg ggcccacccc gcccgtcacc
     1021 gacccgctcg ccggaatcga cctcgccgcc gcggcggcca cacctggaac gtggctagag
     1081 ctctcgatcg ccgccgacca cgaggccgtc gaggccgtgt ccgagatcct gtcgcgcgcg
     1141 gccccgggcg ggaccagcgt cgaacccgcg tacgagctgg tcgacgaggg gctggcggcc
     1201 cgcgtcgacc tcgcgcggcc ggcgttggtg cgcgcacacc tgccggtcct ggacgtcgcg
     1261 gcggttcgtg cggccgtcga gcgggtagag cgtgacctcg gtcatctcca ggccttcggt
     1321 ctgcggccga tcggcgacct cgaggccagt ctcgtgctgg aagccgattg ggcgaacgcc
     1381 tggaagcagc actttccggt gctgcggatc ggccgccgga tcgtgatccg gccgacgtgg
     1441 cggcggcatc ggcgggagcc cggcgacgtg gtgatcgcgc tcgatccggg gatggccttc
     1501 gggacgggcc tgcacccgac gacccggctg tgcctggcgg cgctcgagtc aatggcggat
     1561 cgcgacgtac cgatggccga ggtactcgac gtgggttcgg ggtcgggaat cctgtcgatc
     1621 gccgccgcgc gactgggtgc cggcgatgtg ctcgccctgg acatcgacga gatggccgtt
     1681 gatgcgaccc gcgccaacgc gaagcgcaac gggctcgtcc gtcgcgtgcg ggcacgccag
     1741 ggcagctcgc ccagcggtga gggtccgttc gacatcgtcc tcgccaacct catcgcgtcc
     1801 gtgctgctcg ccctcgccga tgggttgatc ggcgacacgc gtcccggcgg cacgctgctc
     1861 gcctccggca tcttcgagaa ccgcgagacc gaggtcgcgg cggcgttcac gtcccgtggc
     1921 ctgtcgatcg cgaaccgctg ggccgaaggc gactgggtcg cgctcgaggt ccgtcgcccg
     1981 gcctgagcga cggcgacccc ccgagggtga atcggggatg ctgcgctacg atctggcttc
     2041 gatgcccgac ctgtcgtcgc tgttcccggc gatcctcgcg acgcacatca cgctcgcgat
     2101 ctgcctgctc gtcccggcgg tcctgctccc gttcacgctg cgcacgcgat ctgtcgcgcc
     2161 gaacgcgacg ccagcgcagc cgggccgcgt cgtgcgaggc ctctcagcgg tccaggcgca
     2221 cgggacggtc gtgatcggcg ctgggctcgc gctcaccggg atggcgatga tcgtcgtcct
     2281 cggtccgcgg atgctcgagc agccgtggct cctcgtgagc ctggcgacgt acgcggtcgc
     2341 ggccatcgtg gcgttcgtca tccagcgccc gcagctgcgc aagctccagg ccagggacgg
     2401 catcgcgacc gacgccgacc gcgcggcctg gagggatcgc gcccggcggc agcgctacgt
     2461 cgcctacggc ctcacgtcga tggtcggcct gatcgcgttc ctgatgtcga cgaagcccca
     2521 gctgtggtga tgccgcgctc ggcggcgatc gtcaacctcg gctgcaaggt caaccagtcg
     2581 gagatggagg gggcggcgcg cctgctgcgc gaggcggggg ttccgctcgt cgacccggac
     2641 cgggcggccg acctctacct cgtgaacacg tgcaccgtga ccgcgacggc ggacgagaag
     2701 tcacgcgcgg cggtccgccg ggcgaggcgg gcgaacccgc gtgcggagat cgtcgtcacg
     2761 gggtgctcgg tccaggtagg gcccgaggcg ttcgcgaggg tcgaccccga cgcacggctg
     2821 gtgggcaacg acggcaaggc cgggtacctc gccgagctcg aaggtctgct cggcgccgca
     2881 atgcggccga acgaaccgct gcccacgctc tcgggcgtgg acctcgtcgc ggcgcctatc
     2941 gacgggatcg ccgacgaccg cgccgccgtt gagcggaccc gggcgttcgt caaggtccag
     3001 gacggctgct cgttctactg cacctactgc atcatcccgc gagcccgcgg cggggagcgg
     3061 tcgctccacc ccgagacggt cctcgccgac gtccgccggg ccctttccgc aggccaccgc
     3121 gagatcgtcc tcaccgggat caacatcggc acctacgacg gcggctggtc cgaaaggggg
     3181 catcgtggga ctcactcgcg atccgccctc accctggctg gactcgtccg acggatcctg
     3241 gccgagaccg acgtcgagcg gatccgcctg agctcgatcg agccgcagca cgtggacgac
     3301 gagctgctcg aggcttgggt cgtcggcgcc ccgcggacgc taccgcacct ccacctgccg
     3361 ctccagtcgg gcgacgacgg cgtgctgcgc cggatgggcc gtcgctacct gaccgccgac
     3421 tacctgcgcg tcgtggcgcg cgcccgtgag gccatccccg gcgtcgccgt ccacgcggac
     3481 gtcatcgcgg ggttcccgtc cgaagacgac gccgcgttcg cgaggacgct cgccttcgcg
     3541 cgccagatcg agttcgccgg cctccacgtg ttccgctact ccgcccggcc cgggacgccc
     3601 gcgacgcgga tggcgggcca ggtcgacgag cggacgaaga aggcccgcgc gggcgacctg
     3661 ctggcggtgg ccgcagacgc gcgggcggcg ttcgccagac agggtctggg gacggtgacc
     3721 cgcgtcctag cggagaggcg gctgcccgac ggccgatggg tcgggcacgc gggggactac
     3781 gtctcgacgg cagtgaccac gaggcaaggc gaccgcggag acctcgagaa tgccatcctc
     3841 acggtccgcc ggaccgggat cgacggcgat gctgccgacc gcgcgaccgg cgagatcctc
     3901 gccatcgacc cgccccgccg tgcgctgcgg cccgccatcc ccgtgatcgc ccttggagca
     3961 accgcccatg ccggatgact gcctgttctg ccggatcgct gcgggcgaga tcccggcgac
     4021 gaagctccac gaggacgccg acgtcatcgc catccgcgac atcaaccccc aggctccgac
     4081 acacgtgctc gtcatgccgg tggcccacat cgtctcggcc gccgacctga cagacgccga
     4141 cggcccgctg ctcgggcgcc tgttctcgat cgccgcggag atcgcacgca gcgaaggcct
     4201 cgacggcggc tggcgcctgg tcgccaacgt gggccgggac ggcggccagt cggtcgacca
     4261 cctccacctg cacgtgctcg gcggccggcc gatgacctgg ccgcccggat gacggcccct
     4321 gcggcgaacg aggagcggcg cggcggaccg ccggcctggt tcgcgatcat cggcatcggc
     4381 gggctgctcc tcggggtcgt gctgatcgcc gcccaggtgc tcggcatcgg cgtcggcggg
     4441 acggccccgg ctgccacgat cgcgcccacc ggtgaggccg cccagtacac gcacgacctc
     4501 gtcaccaaga ccctgaccga cgcgagcttc caggtcatcg accccctgac cccgtatcgg
     4561 ccgggcgaga gcccagcgct gatcggcgtt ccccggcggt cggtccaggc cgtgctgccg
     4621 tctgaccccg acaagggcta catcgtcatc tacgagctgc cgaccaacgg cgaggccgac
     4681 cggctgggca aggacttcgc cgcctacctc ggctcgggca cgggcgccgt ccaatacccc
     4741 cgcgacgccc agttcgtgct ccgccgcgtc ggcccgacac tcgtgttctt ctcgtggtcg
     4801 ccgctggtct cgccggaccc cgagatggcc cgcctcgcgt cgaccctggc gacgatcggg
     4861 gaacccgtca ccgccccctg atcgacctac gcctcctcgg ccaccggcag cgactcgctg
     4921 atctctgccc aggcgcggac ccggtcgcgc ttgaccttga acgtgtgggt gcgcgggacg
     4981 tccgcctcgg gccagacgcg ccagccggcg atccgctggt ttggcccgag tgagccgttc
     5041 gccgccttga cggccgcatc gatctggcgg cgcacgtcct cgggcgaggc agactcgatc
     5101 gccggcgtcg cgcgcggatc gccgggcacc ccgtgcgtgc caggcgcgag cacgatcgcc
     5161 tcgatccgcc cgggcttcgt ctcgatcgcc accgaatcgc ggatgcccgc cacccgcagc
     5221 gcgttctcga ggtcctcggg gaacacgttg aagccgttgg gcaggacgat gatgtccttc
     5281 ttgcgcccgt ggaggtgcag ccggccctcc ttgtcgaacg ccccgaggtc gccggacatg
     5341 taccagccgt cgtccgtgaa cgcggccttg gtggcctcgg gatcgttcca gtagccgggg
     5401 gtgaggctag ggccgcggaa ctggatctcc ccgtcctcga cgatccgcat ctccagcggc
     5461 ttcggcggcc agccgacgca gcccaagggg
//
LOCUS       DBNHXO010000287         1588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4245990, whole genome shotgun sequence.
ACCESSION   DBNHXO010000287 DBNHXO010000000
VERSION     DBNHXO010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4245990"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1588)
                     /locus_tag="AC162A_06025"
     CDS             complement(<1..>1588)
                     /locus_tag="AC162A_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR5010433.1"
                     /translation="DREKTGVAIGADAINPVNGKRVPIFVADYVLGGYGTGAIMAVPA
                     HDERDFEFARKFGLPIVRVVMPRGADPEADLDAAFIDHTIDEVMVNSGRFTGRPAAEA
                     WHEIVGWLAEQGKGRSAVTFRLRDWLISRQRYWGTPIPVIHCERCGIVPVPDEDLPVV
                     LPDTVDYAGSGVNPLTRDEAFLNVACPSCGGPAQRETDTMDTFIDSSWYWYRYLAPHR
                     PDEPLDPDLVESWTPVEQYTGGAEHAVMHLLYAREFTKMLRDIGLVQQNEPWKRVFNQ
                     GQILGTDGERMSKSRGNVQDPDELVARYGADTVRLYLMFMGPWDQGGPWSPSGIEGPY
                     KFLNRVWTIALDPHGRDPGDRNAGALPAGESEHDARSRMRATAHRTLRDVTTDYEGFR
                     WNTMVAKLMELANLLSRYRGTSVAGLAEWDEAVRLLLLMLAPAAPHITEELWSRLAEA
                     RGEAWSSIHATTWPIVDDTVLVEATHEVPIQVNGKLRDKVTVPTGISEIELEQIVLAR
                     DKVATALGGRQPHRIIHAGGG"
BASE COUNT          214 a          577 c          544 g          253 t
ORIGIN      
        1 ccgccgccgg cgtggatgat ccggtggggc tgcctgccgc cgagcgccgt ggccaccttg
       61 tcgcgggcga gcacgatctg ctcgagctcg atctcggaga tgccggtcgg caccgtgacc
      121 ttgtcgcgca gcttgccgtt gacctggatc gggacctcgt gggtcgcctc gaccaggacg
      181 gtgtcgtcga cgatcggcca cgtcgtcgcg tgaatcgacg accacgcctc gccccgggcc
      241 tccgccagcc gcgaccacag ctcctcggtg atgtgcggcg ccgccggcgc cagcatcagc
      301 agcagcagcc ggaccgcctc gtcccactcc gccagaccgg cgacggacgt cccacggtag
      361 cgcgacagca gattcgccag ctccatcagc ttggcgacca tggtgttcca gcggaagccc
      421 tcgtaatccg tcgtcacgtc gcgcagggtc cggtgcgcgg tcgcgcgcat ccgggaccgc
      481 gcgtcgtgct cggattcgcc ggcgggcagc gcgccagcgt tccggtcgcc cgggtcgcgg
      541 ccgtggggat ccagcgcgat cgtccagacc cggttgagga acttgtacgg gccctcgatc
      601 ccactcgggc tccacggccc gccctggtcc cacggcccca tgaacatcag gtacagccgg
      661 acggtgtccg cgccgtaccg ggccaccagc tcgtcggggt cctggacgtt gccgcggctc
      721 ttgctcatcc gctcgccgtc ggtgcccagg atctggccct ggttgaacac ccgcttccac
      781 ggctcgttct gttgcaccag gccgatgtcg cggagcatct tggtgaactc ccgcgcgtag
      841 agcaggtgca tcaccgcatg ctcggcgccg ccggtgtact gctcgaccgg cgtccagctc
      901 tccaccaggt cggggtcgag cggctcgtcg ggtcggtgcg gggccaggta gcggtaccag
      961 taccaggacg agtcgatgaa cgtgtccatc gtgtccgtct cgcgctgcgc cgggccgccg
     1021 caggatgggc aggcgacgtt gaggaacgcc tcgtcgcggg tcagcgggtt cacgccgctg
     1081 cccgcgtagt cgaccgtgtc cggcaggacg accggcaggt cctcgtccgg cacggggacg
     1141 atgccgcacc gctcgcagtg gatgaccggg atgggcgtgc cccagtagcg ctggcggctg
     1201 atcagccagt cacgcagccg aaaggtgacc gccgaccggc ccttgccctg ctccgccagc
     1261 cagcccacga tctcatgcca cgcttcggcg gccgggcgac ccgtgaaccg gcccgagttg
     1321 accatgacct cgtcgatcgt gtggtcgatg aaggccgcgt cgaggtctgc ctcggggtcg
     1381 gcgcccctcg gcatgaccac ccgcacgatc ggcaggccga acttgcgggc gaactcgaag
     1441 tcgcgctcgt cgtgtgccgg gaccgccatg atcgcgccgg tgccgtagcc gcccagcacg
     1501 taatccgcga cgaagatcgg gacgcgcttg ccgttgaccg ggttgatcgc gtccgcgccg
     1561 atcgcgacgc cggtcttctc gcgatccg
//
LOCUS       DBNHXO010000288         1536 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4046972, whole genome shotgun sequence.
ACCESSION   DBNHXO010000288 DBNHXO010000000
VERSION     DBNHXO010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4046972"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1536)
                     /locus_tag="AC162A_06030"
     CDS             complement(<1..>1536)
                     /locus_tag="AC162A_06030"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5010434.1"
                     /translation="PAIAGAMGVAEPGRPLVESLAEALGRAPSLLVLDTVEHVRAVAP
                     ALTDLLARTPGLTILATSRIAIGVPRERVVWVEPLPVPSEDETDPDALAENPAVALFL
                     ARAAVSRPEIEATPTNAALAAAICRRLDGIPLAIELAAAALRVLAPHQLVDQLENRLA
                     DAGAEAGDEGSTGPARQRSLRAAMDWSIGLLPAPVLCLYRRASVISGTFSVPTAVAIL
                     EGGERRGLAPLGIDVPTGLQQLLAASLLRHEDDGKAFGMLTTVRADAQDRLAQSGEAV
                     AMRWAHAYEVLAVAEEAEGALSTGNDVEALDRLDEAHDDIREALEWSMEQGDGTFALR
                     LSGTLAEFWRTRGHHTEGRLRLAAALSMAVDPPPGVHRKALSGAGLLASYQGDYRLGE
                     AYLREALSIARELDDEEAMAAVLNWLGTNAYGAGDLDAAEAYVSESLEIRRRIGEPAG
                     IAVALNALGGVHHFRGDLDLAREMFVESMALKEGHGTASGRAVSLTNLGLVERDAGRP
                     EAAA"
BASE COUNT          190 a          565 c          587 g          194 t
ORIGIN      
        1 gcggccgcct cgggccggcc ggcgtcgcgc tcgaccaggc cgagattggt cagcgacacg
       61 gcgcggccgc tcgcggtgcc gtgaccctct ttcagcgcca tgctctcgac gaacatctcg
      121 cgagccaggt cgaggtcgcc gcggaagtgg tggacgccgc cgagcgcgtt gagcgccacg
      181 gcgatgcccg ccggctcccc gatccggcgc cggatctcca ggctctcgga aacgtatgcc
      241 tcggcggcgt cgaggtcgcc cgcgccgtac gcgttggtgc ccagccagtt gaggacggcg
      301 gccatcgcct cctcgtcgtc caactcgcgg gcgatcgaga gggcctcgcg caggtacgcc
      361 tcgcccaggc ggtagtcgcc ctggtacgag gcgagcaggc cggcgccgct gagcgccttg
      421 cggtggacgc cgggcggcgg gtcgacggcc atcgacagcg cggccgccag ccggaggcgg
      481 ccctccgtgt ggtgcccgcg cgttcgccag aactcggcga gtgtgcccga cagccgcagc
      541 gcgaacgtcc cgtcgccctg ttccatcgac cattcgaggg cctcgcggat gtcgtcgtgc
      601 gcctcgtcga gccggtcgag ggcctcgacg tcgttgcccg tcgagagggc accctcggcc
      661 tcctcggcga ccgcgagcac ctcgtaggcg tgggcccagc gcatcgcgac ggcctcaccg
      721 ctctgcgcga gccggtcctg ggcgtcggcg cgcaccgtcg tcagcatccc gaacgccttg
      781 ccatcgtcct cgtggcgcag cagggacgcc gccaggagct gctggaggcc ggtcggcacg
      841 tcgatgccca gcggcgcgag cccccggcgc tcgccaccct cgaggatcgc gaccgcggtc
      901 gggacgctga aggtgccgga aatcaccgat gcccggcggt acaggcacag gacgggggcc
      961 gggagcaggc cgatgctcca gtccatcgcc gcccggaggc tgcgctgccg cgcgggcccg
     1021 gtggaccctt cgtcgccggc ctcggcgccg gcatcggcaa ggcgattctc cagctggtcg
     1081 acgagctggt ggggcgcgag gacgcgcagc gccgccgcgg cgagctcgat cgcgagcgga
     1141 atgccgtcga ggcgacggca gatcgcggcc gcgagcgcgg cgttggtcgg cgtcgcctcg
     1201 atctcgggcc ggctgaccgc cgcccgggcc aggaacaggg cgaccgccgg gttctccgcg
     1261 agcgcgtcgg gatccgtctc gtcctcggac gggaccggga gcggctcgac ccagacgacg
     1321 cgctcgcggg ggacgccgat ggcgatccgg ctcgtggcga ggatcgtgag cccgggcgtc
     1381 cgggcgagca gatcggtgag ggcgggtgcg acggcgcgga cgtgttccac ggtgtcgagg
     1441 acgagcaggc tcggcgcccg gccgagcgct tcggccagcg actcgacgag cggcctcccg
     1501 ggctccgcga cccccatcgc accggcgatc gcgggc
//
LOCUS       DBNHXO010000289         2548 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3850219, whole genome shotgun sequence.
ACCESSION   DBNHXO010000289 DBNHXO010000000
VERSION     DBNHXO010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3850219"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="AC162A_06035"
     CDS             complement(<1..889)
                     /locus_tag="AC162A_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HIR5010435.1"
                     /translation="MDRFDKFTDRARKVLTLAQDEAQRFNHNYIGTEHLLLGLVREGE
                     GVAARVLENMNVELAKVRTAVEFIIGRGDRPVVGEVGLTPRAKRVIELAIDEARRLGH
                     NYIGTEHLLLGLVREGEGIAAGVLESLGVNLDKVRHEVIRVLSQSSSAGPAQETKRAS
                     KTPTVDALGINLTEAARADKLDPVIGREKEIERVIQILGRKTKNNPALIGEPGVGKTA
                     IAEGLAHRIVAGDVPDILLNKRVLTLDIGSLVAGTKYRGEFEERLKKIIEELRNTNDA
                     ILFIDELHTLVGAGAAEGAI"
     gene            complement(1094..>2548)
                     /locus_tag="AC162A_06040"
     CDS             complement(1094..>2548)
                     /locus_tag="AC162A_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR5010436.1"
                     /translation="AAQEIAEKLQPYIGRKLRLKILEVNRKANRLILSEKVALYEERR
                     EKRDELFSSLQVGQKVTGSVRSIAPFGIFIDLGGIDGLVHKSELSWNKVNNPEAGYKV
                     GDEVEAEVIDINHERGRISLSIRRLQPDPWESTVADFKVGDVIDGTVTKLVNFGAFVR
                     VREGLEGLIHISELSHQRVAHPGDVVHEGQVLKLKIISLDSERHRLGLSLKQAEEAPA
                     RPAPEAGVAPAVGGEPRAPRARRERPERGYSPSDALSEPEGGIDTTLAAAFAQVRDQL
                     AAAEAGDGPEAIAEEAVAEAEVADEVAEEAVIEAVVAEDVAEEAVIEAVVADEIAEEA
                     VIEAVVAEDVAEEAIAEADEAVVVAKGAAAKAKAADAVAEAKIAESPDDDEIAEAVAE
                     AVEAEEIADEAAAEAEIADEVAEEAVIEALVADEVAEEAVIEAVVAEDVAEDAIVEAV
                     VAEDVAEEAVVEADVAEVVAEDAVAEAEAEAKPV"
BASE COUNT          308 a          941 c          861 g          438 t
ORIGIN      
        1 cgatcgcccc ctcggcggcg cccgccccga ccagcgtgtg cagctcgtcg atgaacagga
       61 tcgcgtcgtt cgtgttgcgg agctcctcga tgatcttctt gaggcgctcc tcgaactcgc
      121 cgcggtactt ggtgccggcg acgagcgagc cgatgtcgag ggtcaggacc cgcttgttga
      181 gcaggatgtc cggcacgtcc ccggccacga tgcggtgggc caggccctcc gcgatggccg
      241 tcttgccgac gccgggctcc ccgatcagcg ccgggttgtt cttggtcttg cggcccagga
      301 tctggatgac ccgctcgatc tccttctcgc ggccgatgac cgggtcgagc ttgtcggcgc
      361 gggcggcctc cgtgaggttg atccccaggg cgtcgacggt cggggtcttg ctcgcgcgct
      421 tggtctcctg ggccggaccg gccgacgagg actgcgacag gacccggatc acctcgtggc
      481 ggaccttgtc caggttcacg ccgaggctct cgagcacgcc cgccgcgatg ccctcaccct
      541 cgcgcacgag gcccagcagc aggtgctcgg tgccgatgta gttgtggccg agcctgcgcg
      601 cctcgtcgat ggccagctcg atgacccgct tcgcccgcgg cgtcagcccg acctcgccga
      661 ccaccggccg atcgccgcgc ccgatgatga actcgaccgc cgtccggacc ttggccagct
      721 cgacgttcat gttctcgagc acgcgcgccg cgacaccctc gccctcgcgg acgagcccga
      781 gcagcaggtg ctcggtgccg atgtagttgt ggttgaaccg ctgcgcctcg tcctgcgcaa
      841 gcgtcagcac cttgcgcgcg cggtccgtga acttgtcgaa gcgatccatc gctgtgctca
      901 tgcgtgcggg ctgcaggcgg ccctgaaagc catcctagca tcccaccctg atcgtggcgc
      961 accccccatt ggaggtccgg gtccggcccc gaccttggtc cggtccccga acggggacgc
     1021 cccgggaatc gaacgacccg ccgggatccg gcgggtcgct gtcgttggcg ggcgctgggc
     1081 ctgaccgacc gggtcagaca ggcttggctt cggcctcggc ttcggcgacc gcgtcctcgg
     1141 cgaccacctc ggccacgtcg gcctcgacga ccgcctcttc ggcgacgtcc tcggccacga
     1201 cggcctcgac gatggcgtct tcggcgacgt cctcggcgac gaccgcctcg atgacggcct
     1261 cctcggccac ctcgtcggcg accagcgcct cgatgaccgc ttcctcggcg acctcgtcgg
     1321 cgatctcggc ctcggcggct gcctcgtcgg cgatctcctc cgcctcgacg gcctcggcga
     1381 cggcttcggc gatctcatcg tcatcgggcg actcggcgat cttggcctcg gcaaccgcat
     1441 cggcggcctt cgccttggca gccgcgccct tggcgacgac cacggcctca tcggcctcgg
     1501 cgatcgcctc ctcggcgacg tcctcggcca cgacagcctc gatcacggcc tcctcggcga
     1561 tctcgtcggc aaccacggcc tcgatgacgg cctcttcggc gacgtcctcg gccacgacgg
     1621 cctcgatcac ggcctcctcg gcgacctcgt cggccacctc ggcctcggcg acggcctctt
     1681 cggcgatcgc ctcgggccca tcgcccgcct cggcggcggc cagctggtcg cgcacctggg
     1741 cgaacgcggc ggcgagggtc gtgtcgatcc cgccctccgg ctcggacagc gcgtcagacg
     1801 gcgagtagcc gcgctcgggg cgctcacggc gtgcgcgcgg tgcgcgcggc tcgccaccga
     1861 ccgccggcgc gacgccggcc tccggggcgg gccgggcggg agcctcctcg gcctgcttga
     1921 ggctgagccc caggcgatgg cgctcggagt cgaggctgat gatcttgagc ttgaggacct
     1981 ggccctcgtg cacgacgtca cccgggtggg cgacgcgctg gtgcgacagc tcgctgatgt
     2041 ggatcaggcc ctcgaggccc tcgcggaccc ggacgaaggc accgaagttc acgagcttgg
     2101 tgaccgtgcc atcgatgacg tcgccgacct tgaagtccgc gaccgtcgac tcccacgggt
     2161 cgggctgcag ccggcggatc gagaggctga tccggccgcg ctcgtggttg atgtcgatga
     2221 cctcggcctc gacctcgtcg ccgaccttgt agccggcttc ggggttgttg accttgttcc
     2281 agctgagctc gctcttgtga accaggccgt cgatgccgcc gaggtcgatg aagatgccga
     2341 acggcgcgat gctgcgcacg ctgccggtga ccttctggcc gacctgcagg ctgctgaaca
     2401 gctcgtcgcg cttctcgcgg cgctcctcgt acagggcgac cttctccgag aggatcaggc
     2461 ggttcgcctt gcggttgacc tcgaggatct tgagccggag cttgcggccg atgtacggct
     2521 ggagcttctc ggcgatctcc tgggcggc
//
LOCUS       DBNHXO010000290         8962 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4445898, whole genome shotgun sequence.
ACCESSION   DBNHXO010000290 DBNHXO010000000
VERSION     DBNHXO010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 8962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8962
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4445898"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..1629)
                     /gene="hydA"
                     /locus_tag="AC162A_06045"
     CDS             complement(232..1629)
                     /gene="hydA"
                     /locus_tag="AC162A_06045"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HIR5010437.1"
                     /translation="MTDLLVRGGTVVTASGSARADVAIDGGRITAVEADLGGLAAGAS
                     EVVDATALLVLPGVIDVHTHTRVASDAEPDRFFADSVAAAFGGTTTFLSFNNPGTGSS
                     PAAARSLRTGLREWLATTSGDAAVDVGLSAVISASHEEPVADLPVLVDAGVPTFKAFM
                     VYDFGVDDATLLALLAAAAGSGGMLEVHCENRTILESLTARHLAAGETGPAFHASSRP
                     PYVEAEATGRAIALARAAGAPLYVVHLSSADALAQVRAARSSGQPVFAETCPHYLTLT
                     DDRYALPPEEAAKYVISPPLRAAGNPDALWDGLADGSLALIATDHVPDRAAVEKQSWR
                     ESFDRISNGGPGIETLLTMAWDRGVAAGRIPVERLADVLSTTPARLFGMPAKGAIEVG
                     RDADLVLFDPAARRTVRAADLHHTSDYTPYEGREVQGAVRSTIVRGSFVVRDGAFVGT
                     RGFGRFVERRLERMA"
     gene            complement(1638..2060)
                     /locus_tag="AC162A_06050"
     CDS             complement(1638..2060)
                     /locus_tag="AC162A_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HIR5010438.1"
                     /translation="MSDLSIRVGDLHFSARWEPEAPRTIAVMRAMLPLRARLIHCRWS
                     GESTWIPYGDLRPDVGWEHHTSHPAPGQLALYPGGISECEIFFPYGACATASKVGQLA
                     ANHFATVLPEDGWDDRLREMGRRCLWDGAQDIEIVELG"
     gene            2077..2412
                     /locus_tag="AC162A_06055"
     CDS             2077..2412
                     /locus_tag="AC162A_06055"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF012785.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033971 - hydroxyisourate hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA];
                     GO_process: GO:0051289 - protein homotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HIR5010439.1"
                     /translation="MASDRPTISTHVLDTGTGAPRAGATVRLWRLGLGDAPAALVVEA
                     KTDGDGRVPDLLGPASLETGAYALEFELGADGFFSSLTVGLQIPDAGRSYHVPLLVAP
                     YGLSTYRGS"
     gene            2417..2944
                     /locus_tag="AC162A_06060"
     CDS             2417..2944
                     /locus_tag="AC162A_06060"
                     /inference="COORDINATES: protein motif:HMM:NF020907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HIR5010440.1"
                     /translation="MTGPPGSGWTLDAPDNDAFVAAVAPLFEGAPRFLARLAAARPFG
                     TAEAMFGRARGIAHAMPEDEQLELIDAHPRLGAPPGSVSAFSFVEQGYGPDAAQVAAA
                     AERPRVADELERLNDAYEAAFGFRYCVFVAGRTREELLPGMEAALASDRDREIARALD
                     AVIDIAADRYARPRD"
     gene            complement(2923..3606)
                     /locus_tag="AC162A_06065"
     CDS             complement(2923..3606)
                     /locus_tag="AC162A_06065"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5010441.1"
                     /translation="MTRGSPTRPLPAAAVFDWDGTIVDTLPLIYRANVVVLGELGIAL
                     SREWFRERYTPDWRASYRELGVPEDRWDALAARWAEEMQAGRPRAIPWARGALRRLAR
                     RGVRLGLVTASTRGVVEPNLRRLNLEGVFETAYFADDVVNGKPHPEALLRALADLGID
                     PADSVYVGDTTVDLEMASSAGAPFEAVAGTTSAAAFWAAGVERVWPGVGHWVDDLLGS
                     RAGQSRGRA"
     gene            complement(3603..4232)
                     /locus_tag="AC162A_06070"
     CDS             complement(3603..4232)
                     /locus_tag="AC162A_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR5010442.1"
                     /translation="MPIDLFLRGLAIGFAVAFALGPIGLLVIKRTVDRGWLYGFLSGT
                     GVATADAAYGAIAAFGLTAITGLLMGIDRWLGIIGGIALLVLAARALRDARSDRPAEA
                     RQDTGRLASPFGAWASMVALTLANPATILSFAALFASIGAGTGGAGGATAVVAGVFLG
                     SVAWWAILTGVIAGLRARLTPRMIQGLNIASGVVIGGFGIAAIIFGLMA"
     gene            complement(4294..5322)
                     /gene="jag"
                     /locus_tag="AC162A_06075"
     CDS             complement(4294..5322)
                     /gene="jag"
                     /locus_tag="AC162A_06075"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HIR5010443.1"
                     /translation="MSEYQEFTGKSVEEAIKSACDVFGVGLGDLDFEILTPGSRGVLG
                     MGAEPARIVAAPRSALGGAAPKREAAEATPLPAVPHEERPYDRDRADRGPRPATDRPD
                     RGPSERGGYDRGPRRDDRPAGGRGGSDRGGYRGGPDRGPRRDDRPRTDAPARGGSELS
                     AAEVAQVTTARGSLAMERAELEAAEATPETLAAGKEILETLMGHLGFEGIAVEIREGE
                     TSRLNVVGDGADREALGSLIGRKGERLSALQHLVNLMLSRRTGQWTRVLVDVEDYRGR
                     RERQLIDLAARAAERVEQTGKMLQLEPMPALERRWIHLALRDNASVSTQSIGDEPNRR
                     VVVLPRNP"
     gene            complement(5324..6499)
                     /locus_tag="AC162A_06080"
     CDS             complement(5324..6499)
                     /locus_tag="AC162A_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HIR5010444.1"
                     /translation="MTRLQPLLVRLAPILFLVVIALVVAACSPLGTTSNGSPTPSPTL
                     SQAPLEPASPDGNPINLLAWLFTPIFQALFIGLVFFYQLTGQNIVLGILLLTIALRLL
                     VIPLYRRQLVSSRQMQLLQPEIRELQRKYKGDRVKSQGAVSEFYKQRGVNPAGGCLPI
                     LLTMGLLIPMYSVFSQGLTNYNPSAMWHVFGINLFPNIACADAPIFNAQGHVINPCLD
                     AVAYGINWGLPEPAITGLIVAGFGISILAIFSSLTQLVASRMTLPPHDPTTQDDQNVK
                     LQRQMAYFLPLISILWGSSLPAGLFLYWITATLIQIVQQYLILGWGGMFPLFGWYPEF
                     ARNHTPRYPVQLPPPKPVPPGQSTSASERSKSVDRDLSARSTIRPNRTRTARRGRRR"
     gene            complement(6519..6728)
                     /gene="yidD"
                     /locus_tag="AC162A_06085"
     CDS             complement(6519..6728)
                     /gene="yidD"
                     /locus_tag="AC162A_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR5010445.1"
                     /translation="MGVTIGVGLIHLYRIVFAWLPSGCRFEPTCSRYTEQAIQKYGLL
                     RGSWMGAKRIGRCHPWNPGGYDPVR"
     gene            complement(6734..7069)
                     /gene="rnpA"
                     /locus_tag="AC162A_06090"
     CDS             complement(6734..7069)
                     /gene="rnpA"
                     /locus_tag="AC162A_06090"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR5010446.1"
                     /translation="MLSRPGDFARLAEGSVTRTHPLLVGRFARTDLDQTRFGLATGKR
                     LGGAVVRNRVRRRIREALRVMAPSFQPGWDVLIIARPALVAADHDALVGALNRVLRSG
                     GVLGGRTKS"
     gene            complement(7105..7242)
                     /gene="rpmH"
                     /locus_tag="AC162A_06095"
     CDS             complement(7105..7242)
                     /gene="rpmH"
                     /locus_tag="AC162A_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009526048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR5010447.1"
                     /translation="MGKPTFQPKKRHRAKEHGFRARMKTAGGRRVLATRRARGRKKLS
                     A"
     gene            7742..>8962
                     /gene="dnaA"
                     /locus_tag="AC162A_06100"
     CDS             7742..>8962
                     /gene="dnaA"
                     /locus_tag="AC162A_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR5010448.1"
                     /translation="MDAKQVWRAALGELQVSLSPANYETWLRDTSLVDVDDQRFRIAV
                     PNGFAKDWLETRYRSLISQTLARVVGYSVQVEFVVVASGNGDASAPTTETVTQSVRVE
                     PTRVGAPEGAAAGANNLNQRYTFSNFIVGSANRLAHAASLSVAERPGHAYNPLFLYGG
                     VGLGKTHLMHAIGNAVAAKFPRKRVVYATSEKFTNEFITSIQQGKIDEFRARYRRIDL
                     LLIDDIQFIADKERTQEEFFHTFNAIHEDGKQIVLSSDRPPKAILTLEERLRSRFEWG
                     LIADLTAPDLETRIAILRAKAEEQGVPITSDVIEFIARKVVSNIRELEGALNRIVAYA
                     SMGAMPVSIELAQAVLSNVLYNPKKRQVTPERITQAVSDYYGVAMEQLRGQKREKSIV
                     VPRQIAMFLMREETD"
BASE COUNT         1325 a         3199 c         3204 g         1234 t
ORIGIN      
        1 taccgccgcc tggaatagcc cgcggaccgc actctttcgc aagatccgaa caacccgcgc
       61 cgaattcggg ggcggcaagg tcctgtctga caggcgcgcc gcgagcgcat ggccggagtc
      121 tgctaacagc cgcctgcgtg cccgggtccg gcggcctccc gggcccggtc aaccgctcga
      181 gacacgttgg gctgttcggt tggtccgaaa aggcgtcgcc ctgagcgcgc ctcaggccat
      241 ccgctcgagg cggcgctcca cgaaccgccc gaacccgcgc gtcccgacga acgccccgtc
      301 acgcacgacg aacgagccgc gcacgatcgt cgagcggacc gcgccctgca cctcccggcc
      361 ctcgtacggc gtgtagtcgc tggtgtggtg caggtcggcg gcccggaccg tgcgccgcgc
      421 cgccggatcg aacagcacga ggtccgcgtc gcgtccgacc tcgattgccc ctttggccgg
      481 catcccgaac aggcgcgccg gcgtcgtcga cagcacgtcg gccagccgct cgacggggat
      541 ccggccggcg gcgacgccgc ggtcccaggc catcgtgagc agcgtctcga tcccgggtcc
      601 accgttcgag atgcggtcga acgactcccg ccagctctgc ttctccaccg cggcccggtc
      661 cggcacgtgg tccgtggcga tcagcgcgag cgagccatcg gccagtccgt cccacagggc
      721 gtcggggttg ccggcggcgc ggagtggcgg tgagatcacg tacttcgcgg cctcctcggg
      781 cggcagcgcg taccggtcgt cggtgagggt gaggtagtgc gggcaggtct cggcgaacac
      841 cggctgcccc gagctcctcg cggcacgaac ctgggccaac gcgtcggcgg acgagaggtg
      901 caccacgtac agcggcgccc cggccgcccg cgccagcgcg atcgcccggc cggttgcctc
      961 cgcttccacg tacggcggtc gcgagctggc gtggaacgcg ggcccggtct ccccggcggc
     1021 gaggtggcgc gcggtcagcg actcgaggat cgtgcggttc tcgcagtgga cctcgagcat
     1081 cccgcccgaa ccggcggcgg cggccaggag cgcgagcagc gtcgcgtcgt cgacgccgaa
     1141 gtcatagacc atgaacgcct tgaacgtggg tacgcccgcg tcgaccagca ccggcagatc
     1201 tgcgaccggc tcctcgtgcg aggccgagat caccgcgctc aacccgacgt cgaccgcggc
     1261 gtcgcccgac gtcgtcgcga gccactcccg gaggcccgtc cggagcgacc gcgcggccgc
     1321 cggcgacgac ccggtccccg ggttgttgaa ggagaggaac gtcgtcgtgc caccgaacgc
     1381 cgccgccacg gagtcggcga agaaccggtc cggctcggcg tcgctggcga cccgcgtgtg
     1441 ggtgtggacg tcgatcacgc ccggcagcac gagcagcgcc gtcgcgtcga cgacctcgct
     1501 ggcgccggcc gccaggccgc ccaggtcggc ttcgaccgcc gtgatcctgc ctccgtcgat
     1561 cgccacgtcg gcccgggcgg accccgacgc cgtgacgacc gtgccgccgc ggacgagcag
     1621 gtcggtcacc ggccgcctca gccgagctcg acgatctcga tgtcctgggc gccatcccac
     1681 aggcagcgcc ggcccatctc gcgcagccgg tcgtcccagc cgtcctcggg gagcacggtc
     1741 gcgaagtggt tcgcggccag ctggccgacc ttcgacgccg tcgcgcaggc gccgtacggg
     1801 aagaagatct cgcactcgct gattccgccc gggtagaggg cgagctgacc cggggcagga
     1861 tgcgacgtgt ggtgctccca gccgacgtcc ggccgcaggt cgccgtacgg gatccaggtg
     1921 gactcgccgc tccagcgaca gtggatgagc ctggcccgaa gtgggagcat ggcccgcatc
     1981 accgcgatcg tccggggtgc ctccggctcc cagcgggcgc taaagtgcag atcgccaacc
     2041 cggatcgaca gatcgctcat cagcggagga tagccggtgg cctcggaccg accgaccatc
     2101 tccacgcacg tcctcgacac cggaacggga gccccgcgtg cgggcgcaac cgtccggctg
     2161 tggcgcctcg gactgggcga cgcgcccgcg gcgctcgtcg tcgaggcgaa gaccgacggg
     2221 gacgggcgcg tccccgacct cctggggccc gcgtcgctcg agacgggcgc ctacgccctt
     2281 gagttcgagc tcggtgccga cgggttcttc tcgtcgctga cggtcgggct gcagatcccc
     2341 gacgccggcc gcagctacca cgtcccgctc ctcgtcgcgc cctacggcct gtcgacctat
     2401 cgggggtcat gaggcggtga ccgggccgcc tggctcgggc tggacgctgg acgcgcccga
     2461 caatgacgcg ttcgtcgctg cagtggcccc gttgttcgag ggcgcgccgc ggttcctcgc
     2521 ccggctcgcc gcggcgcgcc cgttcggcac cgcggaggcg atgttcggcc gtgcccgcgg
     2581 catcgcccac gccatgcccg aggacgagca gctggagctg atcgacgccc acccgcgcct
     2641 cggtgcgcca ccgggatcgg tgtccgcgtt ctcgttcgtc gagcagggct acggccccga
     2701 tgccgcgcag gtcgccgcgg cggccgagcg gccgcgcgtg gcggacgagc tcgagcgcct
     2761 gaacgacgcc tacgaggcgg ccttcgggtt ccgctactgc gtgttcgtcg ctggccggac
     2821 ccgcgaggag ctgctgcccg ggatggaggc ggcgctcgcg tcggatcgcg accgggagat
     2881 cgcccgtgcg ctcgatgccg tgatcgacat cgccgcggat cgttacgcgc gcccgcggga
     2941 ctgaccggcc cggctcccga gcaggtcatc gacccagtgg ccgacgcccg gccacacccg
     3001 ttcgacgccg gctgcccaga acgccgcggc gctcgtggtc ccggcaacgg cctcgaacgg
     3061 cgcgcccgcc gaggacgcca tctcgaggtc gacggtggtg tcgccgacgt acacggagtc
     3121 ggccgggtcg atgccgaggt ccgcgagcgc gcggagcagc gcctcggggt ggggcttgcc
     3181 gttgacgacg tcgtccgcga agtacgctgt ctcgaacacg ccctccaggt tgagccggcg
     3241 caggttcggc tcgacgacgc cgcgcgtcga cgcggtgacg agccccagcc ggacaccccg
     3301 ccgcgccagc cggcgaagcg cgcctcgcgc ccaggggatc gcacgcggcc ggccggcctg
     3361 catctcctcg gcccagcgcg cggcgagcgc gtcccagcgg tcctcgggca ccccgagctc
     3421 gcggtacgag gctcgccagt cgggcgtgta gcgctcgcgg aaccactccc tcgagagcgc
     3481 gatcccgagc tcgccgagca cgacgacgtt ggcccggtag atgagcggca gcgtgtcgac
     3541 gatcgtgccg tcccagtcga acaccgccgc ggcggggagc gggcgggtgg gcgacccgcg
     3601 ggtcatgcca tcagcccgaa gatgatcgcc gcgatcccga acccgccgat gaccacgccg
     3661 gacgcgatgt tcaacccctg gatcatgcgc ggggtgagcc gggcgcggag ccccgcgatg
     3721 acgccggtca ggatcgccca ccaggcgacg gacccgagga acacgcccgc gacgacggcc
     3781 gtcgcgcccc cggcgccgcc cgttcctgcg ccaatgctcg cgaacagtgc cgcgaacgag
     3841 aggatcgtgg ccgggttggc cagcgtgagg gcgaccatcg acgcccaggc gccgaacggc
     3901 gacgcgaggc ggccggtgtc ctggcgcgcc tccgccggcc ggtccgagcg tgcgtctcgc
     3961 agcgcccgag cggcgagcac gaggagcgcg atgccgccga tgatcccgag ccagcggtcg
     4021 atgcccatca ggagcccggt gatcgccgtc agtccgaatg ccgcgatcgc cccatacgcg
     4081 gcatcggccg tcgcgacgcc cgttcccgac aggaacccat acagccagcc gcggtcgacc
     4141 gtacgcttga tcacgagcaa cccgatcggg ccgagggcga acgcgacggc gaacccgatc
     4201 gcgaggccgc gcaggaacag gtcgatgggc atcgaggtcg ccgccatcgg tcaggcgggc
     4261 cggcgacgcc ttacggcaag ggcgaccacc cggctacggg tttcgcggca gcaccacgac
     4321 gcggcgattc ggttcgtcgc cgatggactg ggtcgacacc gaggcgttgt cgcgcagcgc
     4381 caggtggatc cagcggcgct cgagagccgg catcggctcg agctggagca tcttgcccgt
     4441 ctgctcgacg cgctcggcgg cacgcgccgc gaggtcgatc agctggcgct cgcggcgacc
     4501 gcggtagtcc tcgacgtcga cgagcacgcg cgtccactgg ccggtgcggc gagagagcat
     4561 caggttgacc aggtgctgga gcgccgacag ccgctccccc ttgcgcccga tcagcgaccc
     4621 gagcgcctcc cggtcggcgc cgtcgccgac gacgttgagg cggctggtct cgccctcccg
     4681 gatctcgacg gcgatgccct cgaagccgag gtggcccatc agcgtctcga ggatctcctt
     4741 gccggcggcc agcgtctcgg gcgtcgcctc ggcggcctcg agctcggcgc gctccatcgc
     4801 gagcgagccg cgggccgtcg tcacctgggc gacctcggcg gccgacagct cggagccgcc
     4861 gcgggccggc gcgtcggtgc gcggccgatc gtcgcgacgg gggccgcgat cagggccgcc
     4921 gcggtagccg ccacggtcgg agccaccacg gccacccgcc ggtcggtcgt cgcgccgagg
     4981 accgcggtcg tagccgccgc gctccgaggg gccgcggtcg gggcggtcgg tggcggggcg
     5041 aggcccccgg tcggcgcggt cgcggtcgta ggggcgttcc tcgtgcggca cggccggcag
     5101 cggcgtggcc tcggcggcct cgcgcttcgg cgccgcccca cccaaggcgc tgcggggggc
     5161 ggcgacgatc cgggcgggct cggcacccat gccgagcacg ccgcggctgc ccggggtgag
     5221 gatctcgaag tcgaggtccc cgaggccgac gccgaacacg tcgcaggcgc tcttgatcgc
     5281 ctcctcgacg gacttgccgg tgaattcctg gtattcgctc atgtcagcgt cgtctccctc
     5341 gtcgcgcggt tcgggttcgg ttgggccgga tggtcgatcg ggcggaaagg tcccggtcga
     5401 cggacttcga gcgctccgag gcgctcgtgg actgcccggg tgggaccggc ttgggcggcg
     5461 gcagctggac cgggtagcgc ggcgtgtgat tgcgcgcgaa ctccgggtac cagccgaaca
     5521 gcgggaacat gccgccccag ccgagaatga ggtattgctg gacgatctgg atcagcgtgg
     5581 ccgtgatcca gtacaggaac aggccggccg ggagcgagct gccccacagg atcgagatca
     5641 agggcaggaa gtaggccatc tggcgctgca gcttcacgtt ctggtcgtcc tgggtcgtcg
     5701 gatcgtgcgg cggcagcgtc atccgcgacg cgaccagctg ggtgagcgac gagaagatgg
     5761 ccaggatgct gatcccgaag cccgccacga tcagcccggt gatggccggc tccggcaggc
     5821 cccagttgat gccatacgcg acggcatcga ggcacgggtt gatcacatgg ccctgggcgt
     5881 tgaagatcgg ggcgtccgcg cacgcgatgt tcgggaacag gttgatgccg aacacgtgcc
     5941 acatggcgct cgggttgtag ttcgtgaggc cctggctgaa caccgagtac atcgggatca
     6001 gcaggcccat ggtcagcagg atcggcaggc agccgccggc ggggttgacg ccgcgttgct
     6061 tgtagaactc gctcaccgcc ccctggctct tgacgcggtc gcccttgtac ttccgctgca
     6121 actccctgat ctcgggctgg agcagctgca tctggcgcga tgacaccagc tggcggcggt
     6181 agagcgggat cacgaggagc cgcagggcga tggtcagcag caggatgccc aggacgatgt
     6241 tctggccggt caactggtag aagaagacga gcccgatgaa cagggcctgg aagatcgggg
     6301 tgaacagcca cgcgaggagg ttgatcgggt tcccgtccgg cgatgccggc tcgagcggcg
     6361 cctggctgag cgtcggcgag ggcgtgggcg agccgttgct cgtggtgccc agcggcgagc
     6421 acgcggccac cacgagggca atgacgacaa ggaacagaat cggcgccagc cggacgagga
     6481 gcggttggag gcgggtcacg cggtgtggtc tcgtgtggtc agcggacggg gtcgtagccg
     6541 ccggggttcc aggggtggca gcggccgatg cgcttggcgc ccatccagct tccgcgaagc
     6601 aacccgtatt tctggatcgc ctgctcggtg tagcgggagc aggtcggttc gaaccggcat
     6661 ccggacggca gccacgcgaa cacgatgcga tacaggtgga tgagtcccac cccgatcgtc
     6721 accccgatgc gcttcacgac ttggtccgac ctccgaggac gcctcccgat cggaggaccc
     6781 ggttcaatgc accgaccagc gcgtcgtggt cggccgcgac gagcgcgggc cgggcgatga
     6841 tgaggacgtc ccagccgggc tggaacgagg gcgccatcac cctgagcgcc tcgcggatgc
     6901 ggcgccggac gcggttccgg accacggccc caccgagccg cttgccggtc gcgagcccga
     6961 accgggtctg atcaaggtcg gtccgtgcga accgcccgac gagcagtggg tgcgtccggg
     7021 tgacgcttcc ctcagccagc ctggcgaaat cgcccgggcg agagagcatg acgagcggca
     7081 gcgagttctg actgcgcccg ccggtcaggc cgagagcttt ttccggcccc tcgcgcgccg
     7141 cgtcgcgaga acgcgccgac cgcccgccgt cttcatgcgg gcgcggaacc catgctcctt
     7201 ggcgcggtgt cgcttcttgg gctgaaacgt cggtttgccc atcgggactg tccgtgatcc
     7261 tctccatcca gccttggacc cggaagctcc gactcctgga ggcacgcaaa cgcgccgagc
     7321 gccaaaaagc gcgacggcgc gtgttcgtcg cgggggatga tatggccgcc ctggaacgag
     7381 tgtcaaacgt gcgacacgct cacggctccg ttccaccggg gttcgtaccg gggttggcgt
     7441 cccacgggcg acggtcggcg gcggggccac cggacgctcc ccgcacgata ctcccaggga
     7501 gtatgcggga atcgccttgc gacggccccg aggcccggtg tattgttccc cagcctcagg
     7561 cgccgggacg ccgcgggaga ccctccgaga ggaagcccga cggacgcccc gcgtggtgcc
     7621 ctgaggatcc ctcctgtggg ttggccgcga cgtcgcctcg gtcaagcccg tggtcggcgc
     7681 tacccagcgc gccgtgctcg tccatcgcag ccttcatcaa cccagggtca cgatcgccgg
     7741 aatggatgcg aagcaagtct ggcgcgccgc gctcggagag ctccaggttt cgctctcgcc
     7801 cgccaactat gagacgtggc tgcgagacac ctcgctggtt gacgtcgatg accagcgatt
     7861 ccggatcgcg gttcccaacg gtttcgccaa ggactggctc gagacccgct accgatccct
     7921 gatctcccag acgctggccc gcgttgtcgg ctacagcgtc caggtcgagt tcgtggtcgt
     7981 cgcgagcggc aacggcgacg cgtctgcccc gaccaccgag accgttaccc agtccgtgcg
     8041 cgtggagccg acccgcgtcg gcgctcccga gggggccgcg gccggcgcca acaacctcaa
     8101 ccagcgctac acgttctcga acttcatcgt gggctccgcc aaccggctcg cccacgccgc
     8161 ctcgctctcg gtcgccgagc ggccgggcca cgcctacaac ccgctgttcc tgtacggcgg
     8221 ggtcggcctc ggcaagaccc acctgatgca cgccatcggc aacgcggtcg ccgcgaagtt
     8281 cccgcgcaag cgcgtcgtct acgcgaccag cgagaagttc accaacgagt tcatcacctc
     8341 gatccagcag ggcaagatcg acgagttccg ggcccgctac cgccggatcg acctgctgct
     8401 gatcgacgac atccagttca tcgcggacaa ggaacggacc caggaagagt tcttccacac
     8461 gttcaacgcg atccacgagg acggcaagca gatcgtgctc tcgtccgatc gcccgcccaa
     8521 ggcgatcctg accctcgagg aacggctccg gtcgcggttc gaatggggcc tgatcgcgga
     8581 cctcacggcc ccggacctcg agacgcggat cgcgatcctg cgcgcgaagg ccgaggagca
     8641 gggcgtgccg atcacgtcgg acgtgatcga gttcatcgcc cgcaaggtcg tgtccaacat
     8701 ccgggagctc gagggcgcgc tgaaccggat cgtcgcgtac gcctcgatgg gcgcgatgcc
     8761 cgtctcgatc gagctcgccc aggcggtcct ctcgaacgtg ctgtacaacc ccaagaagcg
     8821 ccaggtcacg ccggagcgga tcacgcaggc ggtgtccgac tactacggcg tcgcgatgga
     8881 gcagctgcgg ggccagaagc gcgagaagag catcgtggtg ccgcgacaga tcgcgatgtt
     8941 cctgatgcgc gaggagacgg ac
//
LOCUS       DBNHXO010000291         4263 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3851745, whole genome shotgun sequence.
ACCESSION   DBNHXO010000291 DBNHXO010000000
VERSION     DBNHXO010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3851745"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..760
                     /locus_tag="AC162A_06105"
     CDS             212..760
                     /locus_tag="AC162A_06105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5010449.1"
                     /translation="MDRALIERARGGDRDAYSEVATMSIHRLYAVALRVLRDPDAASD
                     AMQAAMVRIWRDLPTLRDPDLYEAWSYRVLLRACSDSRRSARRSVPSIDITGIESPVG
                     DTQSVVAMRDELERAFATLNLNQRAVLVLMYYEGLSVAEIAASLGISVGTVKSRLHGA
                     RNALRAAIDADSRLQVRAERPA"
     gene            757..1971
                     /locus_tag="AC162A_06110"
     CDS             757..1971
                     /locus_tag="AC162A_06110"
                     /inference="COORDINATES: protein motif:HMM:NF023418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR5010450.1"
                     /translation="MISDPDFDRILAAWLAEGPERAPAQDAAAAMQQVDRTSQRHGLL
                     GGRGVGSTLSATGWVAAALVVVVVAAAIAAVGGTSTDTIGRSTDVGAGGTGVSFGSAA
                     RISERWFSDDKPAFTATLPAGTDKGIYWRAATFNAFKLAGWDQTNAREIPVAAGAPLL
                     AGTAEDPDDALTKPIKVTVHPEDFRGNELLSVGTPTEVDLPSTVRVAGVDGWLAGIDL
                     PPGSGDYTVDARLLRLDETDVISSDRLRAAPEVYPEEITALYTGVPAGSLGVDANRLL
                     QQVKDSARSSDPYDLVTEMVRILGDPSVYHYDTDVSDLACTVSQVECLARYKRGYCLH
                     YASTMAMLLRAANPDNPIPTRLVEGFLPGERVGSTETVANHQAHAWVEVYFPGFGWIP
                     FDPTPRGGVISQ"
     gene            complement(1965..2498)
                     /locus_tag="AC162A_06115"
     CDS             complement(1965..2498)
                     /locus_tag="AC162A_06115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5010451.1"
                     /translation="MNHADHVALIRTGVEGGGPRWLELGAGEGAFTLALADVLGPGTR
                     IVALDRDRRALDQMRARVNERFAGARVDTMVGDFTRDLPAGPFDGVLAANSLHFVADL
                     KPVLERIAAALEPGSRFVLVEYDADHGNPWVPHPISFERWTRIAPADGFATPRLIHRV
                     PSRFLNAIYGAVTERGY"
     gene            complement(2516..3904)
                     /locus_tag="AC162A_06120"
     CDS             complement(2516..3904)
                     /locus_tag="AC162A_06120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5010452.1"
                     /translation="MDSTATERTQTATSADRQVRSGDPGYDEARQVWNGMIDHHPAVI
                     ARVESADEVVATIQLAREQGLPISIRGGGHNVAGLAVGNGAVVIDLSALNAVTVDPVK
                     RRARVGGGARWRDLDEAGQAHGLATPGGVVSDTGVAGLTLSGGLGWLRRKHGLSCDAL
                     RGATVVTADGRILHASKDEHPDLFWGLRGGGGNFGVVTEFEFELYPVGPTVATTVAIW
                     PIEQAAAALRDLRDLAPTMPDEISPVAVLSPVPDDESFPESIRGRDGLFILALAATTT
                     DEGAEILRPIAALGGGAAIDIGGPASYAEFQQFFDAEYPAHTMRYYWKSSFAEDMPDA
                     LIDQLVEAFGRRPSHHSTIDIWANGGAIARVSSDDSAFGQRTAPWLISPESNWESADD
                     DAANLAWGREIVASVGGSAYLNFPGLLEEGEQQAKTSHGSTFDRLAQVKRAWDPDNVF
                     RFNANITPASRP"
     gene            4058..>4263
                     /locus_tag="AC162A_06125"
     CDS             4058..>4263
                     /locus_tag="AC162A_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5010453.1"
                     /translation="MPRSATVTRERILDVATDIALDRGFAAASLDEVLSQSGVTKGAF
                     YHYFGSKADLGRAILDRYAKADLET"
BASE COUNT          656 a         1466 c         1481 g          660 t
ORIGIN      
        1 agcgaccttc gtgaccgtcg tgcccgcgcg gctcgcaccg tcccgcgctc gctccgtggc
       61 cgacccggac ctactcttct cggtcgtcgc cgtgaggccg gagtctacca cgcgggattc
      121 cgtgctcagc gatatgaaga tcgaaccgga ccatcgaatc gcgcgccaag atgagagacg
      181 tcggcgcgtg cgtgccgagc gcggagaccc ggtggataga gcactgatcg agcgcgcccg
      241 agggggcgat cgggacgcat acagcgaggt ggcgaccatg tcgatccacc gtctgtacgc
      301 ggtcgctctg cgggtgctcc gggatcccga cgcggcgagt gacgccatgc aggcggcgat
      361 ggtcaggatc tggcgcgacc tgccgacgtt gcgcgacccc gacctgtacg aagcgtggtc
      421 gtaccgcgtg ctgctgcggg catgcagcga ttcgcgccgg agcgcgcgtc gttcggtccc
      481 ctcgatcgac atcaccggca tcgagagtcc tgtcggggat acccagtcag tcgtcgccat
      541 gcgggacgag ctcgagcgtg ccttcgcgac cctcaacctc aatcaacgcg cggtcctcgt
      601 gctcatgtac tacgagggac tgtcggttgc ggagatcgcc gcatcgctcg gcatctcggt
      661 gggcacggtg aagtcgcggc tccatggagc gcgcaacgcg ctccgagccg ccatcgacgc
      721 cgattcgcga ctgcaggttc gggcggagcg tccagcatga tcagcgatcc cgacttcgac
      781 cgcatcctgg ccgcgtggct ggccgaagga cccgagcgag cgcccgccca ggacgcggcg
      841 gctgccatgc agcaggtcga ccgcacgagc caaaggcacg gtctcctcgg gggtcgtggc
      901 gtgggatcga cactctccgc taccgggtgg gtcgccgctg cgctcgttgt ggtcgtggtg
      961 gctgcggcaa tcgcagcggt cggcgggaca tcgactgaca cgatcgggcg ctcgaccgac
     1021 gtgggtgccg gcggcaccgg cgtgagcttc ggatcggccg cgcggatctc ggaacggtgg
     1081 ttcagcgacg acaagccggc gttcaccgcg accctgccag cgggcaccga caagggcatc
     1141 tactggcgcg cggccacgtt caatgcgttc aagctcgcag gctgggacca gacgaacgct
     1201 cgcgagatcc cggtcgcggc cggcgcaccc ctcctggccg gtactgcgga agaccccgat
     1261 gacgccctca cgaagccgat caaggtcacc gtgcaccccg aggacttccg cggcaacgag
     1321 ctgctctcgg ttggcacgcc gaccgaggtc gacctcccga gcacggtccg ggtggccggc
     1381 gtcgacggat ggttggcggg catcgacctg ccgcccgggt cgggcgacta caccgtcgac
     1441 gcgcggctgc tgcgcctgga cgagacggac gtcatcagca gcgatcgcct gcgagcggcg
     1501 cccgaggtct accccgagga gatcaccgcg ctctacaccg gcgtgccggc aggatcgctc
     1561 ggggtcgacg ccaaccgtct gctccaacag gtcaaggact ccgcgaggtc gtccgatccg
     1621 tacgacctcg tcacggagat ggtccggatc ctgggcgacc cgtccgtcta ccactacgac
     1681 acggacgtca gcgacctggc gtgcacggtc agccaggtcg agtgcctggc gcgctacaag
     1741 cgcggctact gcctccacta cgcgtcgacg atggcgatgc tgctgcgcgc ggcaaacccc
     1801 gacaacccga tcccgacccg cctcgtcgag gggttcctcc cgggcgaacg ggttgggtcg
     1861 actgagaccg tggcgaacca tcaggcgcac gcgtgggtcg aggtgtactt cccaggcttc
     1921 gggtggatcc cgttcgaccc cacgccaagg ggcggggtga tctctcagta accgcgctcc
     1981 gtcacggctc cgtagatcgc gttaaggaag cggctcggga cgcggtggat caggcgtggc
     2041 gtcgcgaagc cgtcggccgg cgcaatccgc gtccaccgct cgaacgagat cgggtgcggg
     2101 acccacgggt tgccgtggtc ggcgtcgtat tcgacgagga cgaagcggct gcccggctcg
     2161 agggccgcgg cgatgcgttc gagcactggc ttgaggtccg cgacgaagtg gaggctgttc
     2221 gcggccaaga cgccgtcgaa cggcccggcc ggcagatcgc gagtgaagtc gccgaccatc
     2281 gtgtccacgc gcgccccagc gaaccgctcg ttgactcgag cccgcatctg gtcgagcgcc
     2341 cgccgatcgc ggtccagggc gacgatccgc gtgccgggcc cgaggacatc ggccagcgcg
     2401 agggtgaacg cgccctcccc cgctcccaat tccagccatc ggggaccgcc gccctcgacg
     2461 ccggtccgga tgagcgcgac gtggtcggcg tggttcacgc cgtcaaggat ggacgctagg
     2521 gccgcgatgc gggcgtgatg ttggcgttga accggaacac gttgtcgggg tcccaggcgc
     2581 gcttgacctg ggccagtcgg tcgaacgtcg acccgtggct cgtcttcgcc tgctgctcgc
     2641 cctcttccag gagcccgggg aagttgaggt aggcactgcc gccgacggac gcgacgatct
     2701 ctcggcccca ggccaggttc gcggcgtcgt cgtccgcgga ctcccagttc gactcggggc
     2761 tgatcagcca cggcgcggtc cgctggccga acgcgctgtc gtcgctcgac acgcgggcga
     2821 tcgcgccccc attggcccag atgtcgatcg tcgagtggtg cgatggcctt cgcccgaagg
     2881 cctcgacgag ctggtcgatc agggcgtcgg gcatgtcctc ggcgaaggac gacttccagt
     2941 agtagcgcat ggtgtgggcg ggatattcgg cgtcgaagaa ctgctggaac tcggcgtacg
     3001 acgccggtcc gccgatgtcg atggccgcgc cgcccccgag ggccgcgatc ggccggagga
     3061 tctcggcacc ctcatcggtg gtggtcgctg caagcgccag gatgaacaac ccgtcgcgac
     3121 cccgaattga ttcggggaag gactcgtcat cgggcacggg cgacagcacg gcgacaggcg
     3181 agatctcgtc gggcatggtc ggggcgaggt cgcggaggtc gcgcagcgcc gcggcggcct
     3241 gctcgattgg ccagatggcg acggtcgtcg caaccgtggg gccgaccgga tagagctcga
     3301 actcgaactc cgtgacgacg ccgaagttgc cgccgccgcc acgcagaccc cagaacaggt
     3361 cggggtgctc gtccttgctg gcgtggagga tgcggccgtc cgcggtcacc acggtcgccc
     3421 cgcggagcgc atcacagctg aggccgtgct tccgccgcag ccagccgagg ccgccgctca
     3481 gtgtgagccc ggcaacgccc gtgtccgaga cgacgccgcc cggcgtcgcg agcccgtgcg
     3541 cctgtccggc ttcgtcgagg tcgcgccagc gtgccccgcc gcccaccctc gcccggcgct
     3601 tgacagggtc gacggtcacg gcattcagcg cggacagatc gatcaccacc gcgccattcc
     3661 ccacggccag gccggcgacg ttgtggccgc ctccccggat cgagatggga aggccctgct
     3721 cgcgggcgag ctggatggtc gcgaccacct cgtcggctga ctcaacgcgc gcgatcacgg
     3781 ccggatggtg gtcgatcatg ccgttccaga cctggcgcgc ctcgtcatag cccggatcac
     3841 cgctgcggac ctggcgatcg gccgaggttg ccgtctgcgt gcgctccgtc gccgtggaat
     3901 ccatcgttgc cctccggttc gagtgtgtgg cgtccgcgtc gcgtgcaacg ccttgaagat
     3961 accaactggt cggtatgtta tgagccggct cgagcgtcgt caagccccaa atcatggttg
     4021 cattcgacaa taccgatcgg tctgctaggc ttgagacatg ccccgaagcg ccacggtaac
     4081 ccgcgaacgc atcctcgatg tcgcgacgga catcgcgctt gatcgagggt tcgctgccgc
     4141 gtccctcgac gaggtcctga gccagtcggg tgtcaccaag ggggcctttt accactactt
     4201 cggctccaag gcggacctcg gccgggccat tctcgaccgc tacgccaagg cggacctcga
     4261 gac
//
LOCUS       DBNHXO010000292        12623 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3852129, whole genome shotgun sequence.
ACCESSION   DBNHXO010000292 DBNHXO010000000
VERSION     DBNHXO010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 12623)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12623)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12623
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3852129"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="AC162A_06130"
     CDS             <1..252
                     /locus_tag="AC162A_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR5010454.1"
                     /translation="ESPAIEVIERLRAAGADVHYTDALIPAVHLPDGVALLSVADPSA
                     LETDLVIVHSMHPGVSHDWLAGRRILDPSGRSAEQYVVP"
     gene            242..2278
                     /locus_tag="AC162A_06135"
     CDS             242..2278
                     /locus_tag="AC162A_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR5010455.1"
                     /translation="MFREATVFTVTGPSQGAPDPSPLERKPGSTQAVLRLARDIRGRY
                     LFAIDLVGIVLAAYLALALRLDGLATPFDVPAFPLVVVLLVSARTAVDVWLGLYSRRW
                     RYASVPELERVVGAAALGSLVAVAVFYGTATLGAAGWTEGFPRSFWPIELLLSITFLG
                     AVRFGIRAAADSVRAGGRAAVGDRQATLLYGAGEAGMLVARSARRYQASGVRPVGFLD
                     DDPALAGGIVSGLRVFGGLEALEEAIAATGATNLLITMPSASGVAIRRAMEGALAKQL
                     EVRTVPSVTDLIDGSVDAHRIRRVRVQDLLRRPIVTQHAVNVDDIIRDRTVVITGGAG
                     SIGSELARQVFAIGPRRLVLVDRAESPLYLLQRELEMRRAQGRGSGELRIHLANVASR
                     ASMNRLIAEESPSVIFHAAAYKHVPMMEEHPSDAAYVNMGGTDVLLEAAEAADVDRFV
                     FVSTDKAVRPSSVMGASKRIGEMLVADAARRTGRPYVSVRFGNVLGSTGSVVPIFQEQ
                     LENGEPLTITHPDMTRFFMTIPEAAWLILDAAAMGRNGDLFVLDMGEPVRVMDLARDV
                     IRLAGRDPDSHPIETVGLRPGEKLHEELFYDIESVERTESANVRRAKSSVPPVDVRDE
                     VRRMLALANGGDEALLRRVILGYVLAHDEPPVAHDAAVASPEWMAEPAAIAAVQ"
     gene            2351..2854
                     /locus_tag="AC162A_06140"
     CDS             2351..2854
                     /locus_tag="AC162A_06140"
                     /inference="COORDINATES: protein motif:HMM:NF016763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR5010456.1"
                     /translation="MVLSLVLALVLCGWVARRLGTGRVVAWLLIASIGVILAATLAPR
                     PFGVESHPSGSMTCDLSRIGPASLATYLRFNDPALNIVLFIPLGVALGQLDRRQHRNQ
                     LVAAAVMLPVGIELTQALVVPLGRACQGGDVFDNLTGLFVGLVIGAAFHHGMRSVAPG
                     TGGQSGR"
     gene            complement(2839..3909)
                     /locus_tag="AC162A_06145"
     CDS             complement(2839..3909)
                     /locus_tag="AC162A_06145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5010457.1"
                     /translation="MSAISPAPLVSIITPTYNHGLYIGQCLESVLAQTEPRWEQIVID
                     DGSTDGTPDVVRRYTDARIRYVSQRHRGIAGLGGAYNLALGMARGQYVAILEGDDFWP
                     PDKLETQLPAFENPKVVLSWGLAAQTDPVGKATRLTPGRAKVRRHQRKTAAQTVQLLL
                     EENCIPACTVVCRTSALRAVGGFHQPEGIPNVDYPTWLELCRVGRFAPVNRIVGFYRR
                     HDHQVSVTMTKEMLRNLDVGPAFVEALDVRERKALGVSVDSARRSARRSWAAVGYAAG
                     SVALKEGRRSVATAYFRSALREGGSGIRLRAMVGLASIYLGIDPDSIRGLYRYIRVRQ
                     EANELAAVEQANASETLDQRPD"
     gene            4185..5678
                     /locus_tag="AC162A_06150"
     CDS             4185..5678
                     /locus_tag="AC162A_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR5010458.1"
                     /translation="MNKTPAIDARAGAGQDGVRADVFPRSAFREMEAALSDPLFFGVP
                     LGSTLNDFFVTAFVDGLGDWRRRTKWLRRLLYQRHRIAPKVHRRTGATFDPGRVLVTW
                     SIPSHRYDRLLLPVLERLDEGECAVVHGHDGVAQLVPAGIPHFPFREVKDFDARAWRR
                     EYERCWPSWRRTIRDGCERYAFPEGAFELLSLELMVASQNVLGCLGFLEAHQPSVILT
                     EYDRNRLWSCLVLAARKLGIPSLTLVHGVIERDAFGFSPVLADHILCWGELDRAKLID
                     AGEAPSRVTVAGCPRLNRDLPSPSLEGRRRLGIDHDGPVAMLATSPESNRFALADAFC
                     TAIEQLPGMSGVVRLHPSEDLTSYQTIIVSHPTVSFVENGRASSDEALAAADVVVVRA
                     SGFGSDALVKRKPVVVLSPDQEPTGHDLDLIELAGCPQAHDADELAAVLGRMAQDPAF
                     RAERETAAERFVTRFCAAFGNESADITAAAVRAVADRRESLEPSLLS"
     gene            5889..6617
                     /locus_tag="AC162A_06155"
     CDS             5889..6617
                     /locus_tag="AC162A_06155"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR5010459.1"
                     /translation="MTPFSGSILLLAPHPDDAALSCGALLERGEPIDVLTVFTGVPHP
                     PRRGRWDERCGFADSEEGRRVRCAEELEGLSPGGHRVRFLDVLDGQYLDGPRPDADAR
                     AIGSALADFDGALVAAPVGAGRRPRWFDRALRRVAARGSAGPPHPDHVFVRDAVLDAV
                     EGPVLLYEEFPYLRSGLGDQAATKLAEARGVRAELFIAEVDRSAKARRIAAYTSQLQH
                     HGHPEERFDLPELLRPVERYWLLA"
     gene            complement(7063..8763)
                     /locus_tag="AC162A_06160"
     CDS             complement(7063..8763)
                     /locus_tag="AC162A_06160"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018399644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HIR5010460.1"
                     /translation="MTPKTRRTAAHLLVDCLAAEGCEWVFSVPGEETMDVLEALADHG
                     QVRHVTTRHEQGAAFMADVHGRLTGRPAVAMATLGPGATNLVTGIADAFLDRAPMVAI
                     TGQTGIDKVHKESHQYVDIPRMFEPVTKWTARVEVPGAIPEIVAKAFRVARLEKPGPT
                     HIELPEDIAASAVDPGLEPLVLQAASFPEPTDEAIERAADLIAGAERPLILAGNGVLR
                     RNASPELREFARGLHVPVAVTFMGKGAIDDRSHLSLMAVGLQARDHVLSGFDRADLVV
                     SVGYDPVEYGPSRWNPGGGKRIVHIDTTPAEVDAAYRPQVELIGDICGTLRRLLAALL
                     PRGIGGRDATQRHASREVLVHADLRQELLRELAAYEADDGWPIRPQKAIADLRAVLAP
                     DDIVVSDVGAHKIWVARLYQAYEPNTVIISNGFASMGIALPGAIAASLVHPDRHVVAL
                     CGDGGFLMNVQELETAVRLGVRVTVVIWRDDGYGLIDWKQRNEFGRPFGVDFGNPDFV
                     ALAASFGVAAFRPASAADLRPTLARALDVDGPSLVEVPIDYRENLRLTEHLGALDPGA
                     "
     gene            complement(8807..9373)
                     /locus_tag="AC162A_06165"
     CDS             complement(8807..9373)
                     /locus_tag="AC162A_06165"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR5010461.1"
                     /translation="MTLDVLLIVLIVCVLGIVVLARIMPAVTGGTTFVVGGPSMEPSI
                     PLGSAVHVVPVEASELRAGDVISLQAGMKNAVFTHRIVRVVNLPEGMYVETRGDANTG
                     PDPSLVPMEDILGRVSVSIPMAGFGIALLSSYQGVMFLIAFGLVLLAGAWLLETIEDE
                     QQEAMRRAARQALTAFAPEPQSGPQGAG"
     gene            complement(9527..10111)
                     /locus_tag="AC162A_06170"
     CDS             complement(9527..10111)
                     /locus_tag="AC162A_06170"
                     /inference="COORDINATES: protein motif:HMM:NF023806.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HIR5010462.1"
                     /translation="MDQTATDTSARRRSKRRRALFAILLGASFATLGAGAMSLAIFTD
                     SDASNGSWTSGTIILNVTPATSFNVTNILPGDSGNQTLNVSNTGTGALRYAMTTSATN
                     ADGNGLAAQMDLVIQAGTCAAPGATLYNGPLDAAEFGDPGQGDDAGDRAVASGAADSL
                     CFSWDFPLNSGNSFQNSATTATFTFDAEQTANNP"
     gene            complement(10385..12565)
                     /locus_tag="AC162A_06175"
     CDS             complement(10385..12565)
                     /locus_tag="AC162A_06175"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR5010463.1"
                     /translation="MIDLRPLFAPRSIAIVGASPRSSIAQTVRDNLARMGSETRCHFV
                     NPNYEEAFGQPCYPSLAALPERPDSAILAVNPLRAARFAQEAAEAGIPSLVIPGGGVV
                     EGGEAAAEMQRTVAEIAIRHGVALLGPNCMGAVDWTTNSSTYIGDVNPWLPRGHVAGL
                     AQSGSVTDAFLHAPGTRIGYSRIVSVGAEVVLDLCDYLAYCLDDPETHAVMLFVEGFK
                     RPERFLALADRALALGKPMLAVKVGRSAQAQAAAIAHSGSLAGEDRVTSAALDAAGVI
                     RCADLDEMLEAAELVAGIGRLGRRLRGGRTGVVTVSTGEASLVADLAERTGLDLPPVT
                     PEARDAILLDLPSMGYIGNPLDPWGAAEESLAYRVSFEALAASGAYDVLAVVHDNPFR
                     DLPSEVDVARTVSRALIDATAGRPELLPVYVSLTSNDVSDEVKAFLDAEGGMPMLKGA
                     VEAFAAIARLAAWEVHRAGREAAGPRRPGWPALASDPVAWGIDTVLDPLAMAAARGAP
                     IALPERESLQRLSDAGIAVTAWRAVDAAPDAAIRAWRELGGGPVAIKLDAPSLAHKSD
                     AGGVALSLTDESGITGAVHDLAVAAARSGVGVRGLLVEPMAAPGVELIIGGRRDPVFG
                     PVVLVGLGGILAEVLDDVAVLLAPVHAVEVEVGLAGLRGSALLRGVRGTDAVDVAAIV
                     TLVVALGDLLVADPTIVEIDLNPVIAGPRGAVAVDALVVVDAGA"
BASE COUNT         1808 a         4380 c         4432 g         2003 t
ORIGIN      
        1 gagtcaccgg ccatcgaggt catcgagcgg ctccgcgccg ccggtgccga cgtgcactac
       61 acggatgcgc ttatcccggc ggtgcacctg cccgacggcg tcgcgctgct gtcggtcgcc
      121 gaccccagcg cccttgagac ggacctcgtg atcgtccaca gcatgcatcc gggcgtcagc
      181 cacgactggc tcgccggtcg ccggatcctc gacccgtccg gaaggtcggc cgagcagtac
      241 gttgttccgt gaggccacgg tcttcaccgt gacggggccg tctcagggcg caccagatcc
      301 gagtcctctg gagcgcaaac cgggctccac ccaggcggta cttcggctcg cccgcgacat
      361 ccgtgggcga tatctgtttg cgatcgacct cgtcggcatc gtgctcgccg cctacctcgc
      421 cctcgcgctg cggctcgacg gactcgcgac cccattcgac gtcccggcgt ttcctttggt
      481 cgtcgtcctc ctcgtatcgg cgcgtaccgc tgtcgacgta tggctcggcc tgtacagccg
      541 tcgctggagg tacgcgagcg ttcccgaact cgagcgggtc gtgggcgccg ccgcgctcgg
      601 gtcgcttgtc gcagttgccg tcttctacgg aacagccacg ttgggtgcgg cggggtggac
      661 ggaagggttc ccgcgctcct tctggccgat cgagctgttg ctgagcatca cctttctcgg
      721 ggccgtccgg tttggcatca gggccgccgc cgattcggtc cgggctggcg gtcgtgccgc
      781 ggtcggcgac cgccaggcga cgctgctgta tggcgcgggc gaggccggga tgctggtggc
      841 caggtcggcg aggcgctacc aggcttccgg ggtacgcccc gtcgggttcc tcgacgacga
      901 ccccgcgctg gccgggggca tcgtctccgg gcttcgggtg tttggcggcc tcgaggcgct
      961 cgaggaggcg attgcggcaa ccggtgccac gaatctgctc atcacgatgc cgagtgcatc
     1021 cggcgtcgct atccgacgag ccatggaagg ggcgctggcg aaacagctcg aggtccgcac
     1081 cgtcccctcg gtgaccgacc tgatcgacgg cagtgtcgat gcccatcgca ttcgtcgtgt
     1141 ccgggtgcaa gacctgctcc gtcgcccgat cgtgacgcag cacgccgtca acgtcgacga
     1201 catcatccgg gaccgcaccg ttgtcatcac gggcggtgcg ggctcgatcg ggtctgagct
     1261 cgcgcgccaa gtctttgcca tcgggccccg ccgcctcgtg ctggtcgacc gcgccgagag
     1321 cccgctctac ctccttcagc gcgagctgga gatgcggcga gcccaagggc gagggtccgg
     1381 cgagctgcgc attcacctcg ccaacgtcgc gagccgtgca tcgatgaatc ggttgatcgc
     1441 cgaggaatcc ccgagcgtca tcttccatgc cgcggcgtac aagcacgtgc cgatgatgga
     1501 ggagcatccc tccgacgctg cctacgtcaa catgggcggc accgatgtgc tgctcgaagc
     1561 cgcggaggcc gcggatgtgg accggtttgt cttcgtgtct acggacaagg ccgtccgacc
     1621 gtcaagcgtg atgggcgcga gcaagcggat tggcgagatg ctcgtcgcgg acgcggctcg
     1681 gcggaccggg cgcccctacg tgtcggtccg gttcgggaac gtgttgggct cgaccggaag
     1741 cgtggtaccg atcttccagg agcagctcga gaacggtgag ccgctcacca tcacccaccc
     1801 ggacatgact cgcttcttca tgacgatccc cgaggccgcc tggctgatcc tcgacgcggc
     1861 cgctatgggc cgcaacggcg acctcttcgt gctcgatatg ggtgagcccg tccgcgtgat
     1921 ggatctcgct cgcgatgtca tccgcctcgc gggccgggac cccgacagcc atccgatcga
     1981 gaccgtcggc ctgcggccgg gcgagaagct ccacgaggag ctgttctacg acatcgagag
     2041 cgtcgagcgg accgagagcg cgaacgtccg ccgggcaaag tcatcggtgc cgccggtcga
     2101 tgtgcgcgac gaggtgcgcc ggatgctcgc gctcgccaat ggcggtgatg aggcgctgct
     2161 gcgcagggtg atcctcgggt acgtcttggc gcacgacgag ccaccggtcg cccatgatgc
     2221 tgcggtggcc agcccggagt ggatggcgga gcccgcagcg atcgccgcgg tccagtaggg
     2281 cactctgcta ctgcgtcccc tggtcgggcc gctcctcgat ggacgtggcg acccgccgat
     2341 catcctcggt gtggtgctgt ccctcgtgct cgccctggtg ctgtgcggct gggtcgctcg
     2401 ccggctcggg accggcaggg tcgtggcgtg gctcctcatc gcgagcatcg gggtgatcct
     2461 ggccgcgacc ctggcgccac gaccgttcgg tgtcgagtcc cacccgagcg gcagcatgac
     2521 ctgcgacctg tcccggatcg ggccggcgtc cctggcgacc tacctgcggt tcaatgaccc
     2581 cgccctcaac atcgtcctgt tcatccccct gggggtcgcg ctcggacaat tggaccgccg
     2641 ccagcaccgg aaccaactgg ttgccgcggc cgtgatgctg ccggtcggga tcgagctcac
     2701 ccaggctctc gtggtgccgc tcggccgcgc gtgccagggc ggcgacgtgt tcgacaacct
     2761 gacggggctc ttcgtgggcc tcgtgatcgg tgccgctttt caccacggga tgcgatccgt
     2821 ggcaccaggc actggcggtc agtctggtcg ctgatccagc gtctccgacg cgttggcttg
     2881 ttccaccgcc gccaattcat tcgcttcttg gcgaacccgg atgtaccggt acagaccccg
     2941 gatcgagtcc gggtcaatgc cgaggtagat ggacgcgagg ccaaccatgg cgcggaggcg
     3001 tattccgctg ccgccctcac gcaatgcgct acggaagtac gccgtcgcca cgctccgccg
     3061 accttctttc aaggcgacgc ttcccgcggc gtatcccact gccgcccagc ttcgccgcgc
     3121 ggaccgccgc gctgagtcaa cagacacacc cagcgccttc cgctcgcgca catccagtgc
     3181 ctcaacgaac gccggcccca cgtccaggtt acgcagcatc tcctttgtca tcgtcacgga
     3241 gacttggtga tcgtggcgac ggtagaaccc cacgatccgg ttgaccggtg cgaaccgccc
     3301 gaccctgcac aactcgagcc acgtcggata gtcgacgttc gggatgcctt cgggctggtg
     3361 aaagccaccg accgcacgca gcgcgctcgt tcgacacacc accgtgcacg cgggtatgca
     3421 gttctcctcg agcagcagct gaacggtctg ggccgcagtc ttgcgctgat gcctgcggac
     3481 ctttgctcgt cccggggtga ggcgcgtggc cttccccacc ggatcagtct gcgccgcgag
     3541 gccccacgag agcacgacct tcgggttctc gaatgcaggg agctgcgtct cgagcttgtc
     3601 gggtggccag aagtcgtctc cctccaggat cgcgacatac tgcccacgcg ccatcccgag
     3661 ggccaggttg tacgcgcccc ccagacccgc gatcccacgg tggcgttgag agacatagcg
     3721 aattcgtgcg tccgtgtagc gacggacgac gtcaggtgta ccgtcagtcg aaccgtcgtc
     3781 aatgacgatc tgttcccagc gcggctcagt ttgggcaagg acactctcga gacactgacc
     3841 gatgtagaga ccgtggttat aggtcggcgt gatgatgctg acgagggggg cgggcgatat
     3901 tgcgctcaac gacgtctgat ccctccgtcg aagtggtccg tcccgcggcc ggatgacggc
     3961 gtccctccgt gcccgatgcg cgagtgtacg cccgcagcga gtggcgtctt cccggcagag
     4021 gcggcactcg agcgtggcgg tcaggggtcg gtctcggtcc ctgggcgggc gcgacgccaa
     4081 cagggatccg tgagtcacac ggccctagcc cggaacctcg gtatgctgcg tcgaccagct
     4141 ctggaccaag agcgcatcgt tgccgccgat cgagagcctc actcatgaac aagacgcccg
     4201 cgatcgacgc tcgcgccggt gcaggtcagg acggcgttcg ggccgacgtg ttcccgcgct
     4261 ccgccttccg cgagatggag gccgcgctgt cggacccgct cttttttgga gttcccctgg
     4321 gctcgacgct caacgacttc ttcgtcacgg ccttcgtcga cggactcggg gactggcgac
     4381 ggaggacgaa gtggctgagg cgcttgctgt atcagcggca ccggatcgcg cccaaggtac
     4441 atcggaggac gggagccacc ttcgatccgg ggcgcgttct cgtgacctgg agcatcccca
     4501 gccaccgata tgaccgcttg ttgttgccgg tcctcgagcg gctcgatgag ggggagtgcg
     4561 ctgtggtgca cggccacgat ggagtggcgc agttggtgcc cgccggcatc cctcattttc
     4621 ccttccggga ggtcaaggac ttcgacgcgc gggcctggag gcgggaatac gagaggtgct
     4681 ggccatcatg gagacgcacc atcagggacg gctgcgaacg atacgccttc ccagaaggtg
     4741 cattcgagct cctgtccctc gagttgatgg tcgccagcca aaacgtcctt ggttgcctgg
     4801 gcttcctgga ggctcaccaa ccttccgtga tcctcaccga atacgatcgc aaccggctct
     4861 ggtcctgcct ggtgttggct gcgcggaagc tcgggatccc ctcgctgacg ctggtccatg
     4921 gcgtcattga acgtgatgcc ttcggattct cgccggtcct tgccgaccat atcctctgtt
     4981 ggggcgagct cgaccgagcg aaactcatcg atgcgggcga agcgccgtcc agggtgacgg
     5041 tggcggggtg cccgcgattg aatcgggacc tgcccagccc atcgctcgag gggcggcgaa
     5101 ggctcggaat tgatcacgac gggccggtgg ccatgctggc gacttccccc gagagcaaca
     5161 gatttgcgct tgccgatgcg ttctgcacgg ccattgagca gctcccgggt atgtcggggg
     5221 tcgtcagatt gcatccatcg gaggatctga cctcctatca gacgatcatc gtcagccatc
     5281 cgacggtctc cttcgtggag aacgggaggg cttcctcgga tgaagcgctt gcggcggccg
     5341 atgtcgtcgt ggtgcgcgcg agcgggttcg ggagcgacgc cctggtgaaa cgaaaaccgg
     5401 tggtggtcct cagccccgac caggagccga cgggtcacga ccttgatctc atcgagctgg
     5461 caggctgtcc gcaggcgcac gatgcggacg aattggcagc ggttcttggc cggatggcgc
     5521 aggatccggc gttccgtgcg gagcgtgaga ccgccgcaga acgcttcgtc acgcgattct
     5581 gcgcggcctt cgggaacgag tcggcggata tcaccgcggc tgcggttcgc gcggtggctg
     5641 accggagaga gtctctcgag ccatcccttc tcagctaacc gggccgagcg tcgacgtcgg
     5701 gacaattcga cgccggtaca ctactgaaca ctcgccggcg agcggccgcc ggaacggctc
     5761 cctccacggt ctctcgcctg ggcggatgta gtcgaagata tcccggaggg tggcgtgcag
     5821 gtctcggccg ttgcggcgtc aataggggca tcgcctcact ttccacatgt tagctttcgc
     5881 gccttcccgt gaccccgttt tcaggttcga tcctgctcct cgctcctcac ccggacgacg
     5941 cggcgctgtc gtgcggcgct ctcctcgagc gaggagagcc gatcgacgtg ctgaccgtct
     6001 tcaccggcgt cccccatccg ccgcggcgcg ggcggtggga cgagcgctgc ggcttcgccg
     6061 actcggagga ggggaggcga gtgcggtgtg ccgaggagct cgagggcctg tcgccaggcg
     6121 gtcatcgagt ccggttcctg gacgtgctcg acggccagta cctcgacggc cctaggcccg
     6181 acgccgacgc gcgtgcaatt ggatcggctc ttgccgactt cgatggcgcc ttggtcgcgg
     6241 cgcccgtggg ggccggccgc aggccgcgtt ggttcgaccg ggcgctgcgg cgtgtcgcgg
     6301 cccgcgggag cgccggtccc cctcaccccg accacgtctt cgtccgagac gcggtgctcg
     6361 acgcggtcga agggccggtg ctgctctacg aggagttccc ctatctgcga agcggcctgg
     6421 gcgaccaggc cgccaccaag ctggccgagg ctcgcggcgt ccgggcagag ctctttatcg
     6481 cggaggtcga ccggagcgcg aaggcccgcc ggatcgccgc ctacacgagc cagctccaac
     6541 accacggcca ccccgaggag cggttcgacc tcccggagct cctacggccg gtcgagcgtt
     6601 actggctcct cgcttagacg caacgatgcg gtttctgcgc ttcccgcgct ccatggtgcc
     6661 caggggttga tggtcgggtg gctcgggcat acgccgaagg cacgccacgc caccctccgc
     6721 aagcccgaca tcgcgcacgc cctagtcgct ccatgactgc gccgtgcaca gcgcggccgc
     6781 ttggccagtc ccgcggcgtc ggatcgaccc agggatggct cgatttgacg cgcttgtccg
     6841 ccatcgcagt cgccgactga ttgacggtac ccgtaaggca agccacccgg acggtgcgcc
     6901 tcgcgttcac cccctgattg ccgtccgtgg agtcatgcag cgattacccg cggcgttcag
     6961 ctgcgtggct ggcgaaggaa gcgccgattg ggcatccggg cggctcgcgg tggcccattg
     7021 gccgtttgct ggtgctcgct gagcggcttt gcccgcacga cgtcaggcgc ccgggtcgag
     7081 cgccccgagg tgctccgtga gccgcaggtt ctcgcggtag tcgatcggca cctcgaccag
     7141 cgacggtccg tcgacgtcca gcgcccgggc gagcgtcggc cgcagatccg cggcagacgc
     7201 aggccggaac gcggccacgc cgaaggacgc cgccagcgcg acgaagtccg ggttgccgaa
     7261 gtccacgccg aacggccgcc cgaactcgtt gcgctgcttc cagtcgatca gcccgtagcc
     7321 gtcgtcgcgc cagatcacga ccgtcacccg cacgccgagc cggaccgccg tctcgagctc
     7381 ctgcacgttc atcaggaacc cgccgtcgcc gcacagggcc accacgtggc gatccgggtg
     7441 caccagcgac gcggcgatcg cccccggcag cgcgatgccc atcgacgcga agccgttcga
     7501 gatgatcacg gtgttgggct cgtacgcctg gtacagccgc gcgacccaga tcttgtgggc
     7561 cccgacgtcg ctgaccacga tgtcgtcggg cgcgaggacc gcccgcaggt cggcgatcgc
     7621 cttctggggc cggatcggcc agccgtcgtc cgcctcgtac gccgccagct cccgcaacag
     7681 ctcctggcgc aggtcggcgt ggacgagcac ctcgcgtgat gcgtggcgct gcgtcgcgtc
     7741 ccggcctccg atcccgcgcg gcagcagggc ggcgagcagg cgccggagcg tgccgcagat
     7801 gtcaccgatg agctcgacct gcgggcgata cgccgcatcc acctcggcgg gagtggtgtc
     7861 gatgtggacg atccgcttgc cgccacccgg gttccaccga ctggggccgt actcgaccgg
     7921 gtcgtagccg accgagacga cgaggtcggc gcggtcgaag cccgacagga cgtggtcccg
     7981 tgcctggagc ccgaccgcca tcagcgacag gtgggagcgg tcgtcgatcg cccccttgcc
     8041 catgaaggtg accgcgacgg gcacgtgcag cccgcgggcg aactcgcgca gctccggcga
     8101 ggcgttccgg cgcagcaccc cgttgccggc gaggatcaag ggtcgttcgg cgccggcgat
     8161 gaggtcggcc gcgcgttcga tggcctcgtc ggtcggctcg gggaaggagg ccgcttgcag
     8221 caccagcggc tcgagcccgg gatcgaccgc gctcgcggcg atgtcctcgg gcagctcgat
     8281 atgggtcggg ccaggtttct cgaggcgcgc gacgcggaac gccttggcga cgatctcggg
     8341 gatcgcgccc gggacctcga cgcgcgcggt ccacttggtg acgggctcga acatccgcgg
     8401 gatgtcgacg tactggtgcg actccttgtg gaccttgtcg atccccgtct gcccggtgat
     8461 ggcgaccatc ggggcgcgat cgaggaacgc gtcggcgatc ccggtgacga ggttcgttgc
     8521 cccgggcccg agggtggcca tggcaaccgc cggacggccg gtgaggcggc cgtggacgtc
     8581 ggccatgaac gcggcgccct gctcgtggcg tgtcgtcacg tgacggacct ggccgtgatc
     8641 cgcgagcgcc tcgaggacgt ccatcgtctc ctcgccgggc accgagaaca cccactcgca
     8701 gccctcggcc gcgaggcagt cgacgaggag gtgggccgcg gtgcggcggg tcttcggggt
     8761 caccccgtga gtctacgggc tggggtcggc cccggcggtg gtgggatcag ccggcgccct
     8821 ggggcccgct ctgcggctcc ggggcgaagg cggtaagcgc ctggcgcgcg gcgcggcgca
     8881 tcgcctcctg ctgctcgtcc tcgatggtct cgagcagcca cgcgccggcg agcaggacga
     8941 ggccgaacgc gatgaggaac atcacgccct ggtacgagct gagcagcgcg atcccgaacc
     9001 cggccatcgg gatcgagacc gacacgcgac ccaggatgtc ctccatcggg acgagcgacg
     9061 ggtcggggcc ggtgttggcg tcgccgcgtg tctcgacgta catcccctcg ggcaggttga
     9121 cgacccggac gatccggtgc gtgaacacgg cgttcttcat gccggcctgg aggctgatca
     9181 cgtcacccgc gcgcagctcg gacgcctcga cgggcacgac gtgcacggcg gacccgagcg
     9241 ggatcgacgg ctccatcgac ggtccgccga ccacgaaggt ggtcccgccc gtgacggccg
     9301 gcatgatccg ggcaaggacc acgatcccca gcacgcacac gatgagcacg atcaacagga
     9361 cgtccagcgt catgcgcgcg gctcgtgtca tcacggcgac gagcggcata tggcgaggct
     9421 cccgacgggg tgatcggtcg cggagcggcc ggtcggcagg gctgaaatgg atgatgccct
     9481 ggcccgcccg ggagggcgga ccaggacgtt ggcgtcgagc gagtgactac gggttgttgg
     9541 cggtctgctc tgcgtcgaag gtgaacgtgg ccgtggtggc cgagttctgg aagctgttgc
     9601 cgctgttgag cgggaagtcc cagctgaagc agaggctgtc ggcggcaccc gaagcgacgg
     9661 cccggtcgcc ggcgtcgtcg ccctggcccg ggtcgccgaa ctcggcggcg tcgagggggc
     9721 cgttgtagag ggtcgcgccc ggggcggcgc aggtaccggc ctggatcacg aggtccatct
     9781 gcgcggccag gccgttgccg tccgcgttcg tggcgctggt cgtcatcgcg tagcggaggg
     9841 cgccggtgcc cgtgttcgac acgttgagcg tctggttgcc gctgtcgccg ggcaggatgt
     9901 tggtcacgtt gaacgacgtc gccggggtga cgttgaggat gatcgtcccg gacgtccagc
     9961 tcccgttgga ggcgtcgctg tcggtgaaga tcgcgagcga catcgccccc gcgccgagcg
    10021 tcgcgaacga cgcgccgagc aggatcgcga atagcgctcg gcgacgcttc gatcggcggc
    10081 gggccgacgt gtcggtggcg gtctggtcca tgtacgggca ctcccttttc aatcggtgcg
    10141 tgctcgtcgg tccgccaacc cccgatggcg ttggggaccc cggaagggct ctgaccccgg
    10201 aactcgacgc gccgcgcgtg tgtgatcgaa ttcgacacca gcgcgggccg cgcggctaca
    10261 cgaccgggga cccatgcgtg ggggtacctt cgtcactcag gcccggtgcg gtcggacacc
    10321 gttcgtgctc gagatcgggt tggatgacgt tgccggggcg ccccacgcgc cggccggcgg
    10381 catctcaggc tccggcgtcc accacgacca gggcgtccac cgccacggcg ccacgcggtc
    10441 cggcgatcac cgggttgagg tcgatctcga cgatcgtggg gtccgccacg agcaggtcgc
    10501 ccagcgcgac gaccagggtg acgatcgccg cgacatccac ggcgtcggtc cctcggacgc
    10561 cgcgcagcaa cgcggagccg cggaggcccg cgagccccac ctcgacctcg accgcgtgca
    10621 cgggtgccag cagcaccgcg acgtcgtcga gcacctcggc caggatcccg cccagcccga
    10681 ccaggaccac gggcccgaac acggggtcgc gtcgcccgcc gatgatcagc tcgacccccg
    10741 gggcggccat cggctccacc agcaggcctc gcacgccgac gcccgaccgc gcggcggcga
    10801 ccgccaggtc gtgcacggcc ccggtgattc cggactcgtc ggtcagggac agcgccacgc
    10861 cgccggcatc gctcttgtgg gcgagggatg gggcgtcgag cttgatcgcg accggcccgc
    10921 cgccgagctc acgccaggcg cggatcgcgg cgtcgggtgc cgcatcgacc gcgcgccagg
    10981 ccgtcacggc gatgcccgcg tccgacagcc gctgcaggct ctcgcgctcc ggcagggcaa
    11041 tcggcgcgcc cctcgccgcc gccatcgcga gcggatcgag gaccgtgtcg atgccccagg
    11101 cgactggatc cgacgccagc gccggccagc ccggtcgccg cggaccggcc gcctcacggc
    11161 ccgcgcggtg gacctcccaa gcagccagcc gggcgatcgc ggcaaacgcc tccacggcgc
    11221 ccttgagcat cggcatcccg ccctcggcgt cgaggaacgc cttgacctcg tcgctcacgt
    11281 cgttcgaggt cagcgacacg tagaccggca gcagctcggg gcggccggcc gtcgcgtcga
    11341 tcagggcgcg gctgacggtc cgcgcaacgt cgacctcgct gggcaggtcg cggaacgggt
    11401 tgtcgtgcac gaccgcgagc acgtcgtatg cgcccgatgc cgcgagggcc tcgaacgaga
    11461 cccggtaggc gagggactcc tccgccgcgc cccagggatc gagcgggttg ccgatgtagc
    11521 ccatcgatgg caggtcgagg aggatcgcgt cgcgggcctc gggggtcacc ggcggcaggt
    11581 cgaggccggt ccgctcggcg aggtcggcca ccagcgacgc ctcgccggtc gacaccgtca
    11641 cgacgcccgt ccgcccgccg cgcagccgcc ggccgagtcg cccgatgccc gcgaccagct
    11701 ccgccgcctc gagcatctcg tccagatcgg cacaccggat gacgcccgcc gcatcgagcg
    11761 cggccgacgt gacccggtcc tcgcccgcca ggctgccgct gtgggcgatc gcggccgcct
    11821 gggcctgggc cgaccgcccg accttgaccg ccagcattgg cttgcccagc gcaagcgcgc
    11881 ggtccgcgag cgcgaggaag cgctcgggcc gcttgaaccc ttcgacgaac agcatgacgg
    11941 cgtgcgtctc gggatcgtcg aggcagtagg cgaggtaatc gcacaggtcg agcacgacct
    12001 cggcgcccac cgacacgatg cggctgtacc cgatccgggt gcccggcgcg tggaggaagg
    12061 cgtcggtcac gctgcccgac tgggcgaggc cggcgacgtg gccgcgcggc agccacgggt
    12121 tgacgtcgcc gatgtaggtc gagctgttgg tcgtccagtc gacggccccc atgcagttcg
    12181 gccccagcag cgcgacgccg tgccggatcg cgatctccgc gacggtgcgc tgcatctcgg
    12241 cggccgcctc gccgccctcg accacgccgc cgcccgggat cacgaggctc gggatgccgg
    12301 cctcggccgc ctcctgcgcg aaccgggctg cccgcagcgg gttgaccgcg aggatcgccg
    12361 agtcggggcg ctcggggagg gcggcgaggc tcgggtagca cggctgcccg aacgcctcct
    12421 cgtagttggg gttgacgaag tggcagcgcg tctcgctgcc catccgggcg aggttgtcgc
    12481 ggacggtctg ggcgatcgac gagcgcgggc tcgcgccgac gatcgcgatc gaacgcggcg
    12541 cgaacagggg gcggaggtcg atcacgccgc ggggagggtc agccgacgac cgcgccgacg
    12601 ccacgcttct cgagcgagcg ggc
//
LOCUS       DBNHXO010000293         5003 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2462951, whole genome shotgun sequence.
ACCESSION   DBNHXO010000293 DBNHXO010000000
VERSION     DBNHXO010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2462951"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..1186
                     /locus_tag="AC162A_06180"
                     /pseudo
     CDS             371..1186
                     /locus_tag="AC162A_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
     gene            complement(1140..4010)
                     /locus_tag="AC162A_06185"
     CDS             complement(1140..4010)
                     /locus_tag="AC162A_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR5010464.1"
                     /translation="MSVLPGVGGSSGVQVEGVPALRRVPDPWAVDAPEVVAALDSDVT
                     RGLDVDEARHRLIACGPNELVERAAKSAWRLLAEQFTNAMILVLLGAAAITAAIGDLK
                     DTAIILVIVALNGIIGFVQEYRAEQAMDALKRMTSPSARVVRAGVTIVVPATELVPGD
                     LVRLDAGDVVTADLRLLEARSLRINEAALTGESEPASKVTAPLANVTAAVLGDQRNMA
                     FSGTAVTYGRGLGLIVATGMETALGRVAELLEEHGSGQTPLQRRLSTLGKRLAFAAFL
                     VCIFVFVSGVARGEPADVMFLVAVSLAVAAIPEGLPAVVTIALALGARRMAQRHALVR
                     KLPAVETLGSVTVICSDKTGTLTENRMLAERVWTPNGDYRVTGDGYSPTGRFDPQPAN
                     DTALERLARVAAACNDSSLHAPATSDDPWTISGDPTEAALLALAAKLDVDPDAVAAAS
                     PRVEELTFDPERRRMATIHRAGADVWVAVKGALEAIGPLLDAHEADLLRQAEHVAERY
                     ALDGYRVLAFAERTIDDVPARLDDAEHGLHLLGIVGMADPPREASAPSIAACRAAGIT
                     PIMITGDHPLTGNAIARRIGILRDGGRSLTGAELEQLDNVAFDVSVADVAVYARTNPE
                     QKLRIVDAWRRRGAVVAMTGDGVNDAPALRRADIGVAMGITGTEVSKEAADMVLADDD
                     FATIVAAVEEGRRIYDNIRRFVRYLLTTNSAEIWVMTLAPLLGLPFPLLAVQILWVNL
                     VTDGLPALALGVEPTERGTMRRPPRSPSESLFARGLWQHTIWVGLLMASVALAVQAYA
                     ISQGWPWQTMVFATLAFLQLGHALAIRSESESLFRLGLRSNMPLTIAVAATFVVQLAL
                     IFIPALQPIFGTVALSMPQLAVVLVASTSGFAAVEVEKWVGRRTSGPTSAGHGERGAT
                     VRRQRRLAARSGPRLPSAEARFARRFREATGQDHAVSATPSR"
     gene            4189..4533
                     /locus_tag="AC162A_06190"
     CDS             4189..4533
                     /locus_tag="AC162A_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5010465.1"
                     /translation="MGNEQVAPETKGVVVSELAAVDLGGEIEGMAGRQLRMRMVTIEP
                     GGVFGPIHDHAGRPGIVYVLQGTITDHRDGVATDYGPGMGWPEDRNTTHWLENGGTIP
                     AVEISVDIVRRE"
BASE COUNT          789 a         1704 c         1773 g          737 t
ORIGIN      
        1 cgatcgcttc gagctggacg tcgaacgccg ccgtcgtcag gcagaacccg ggtgggatcc
       61 ggaaccccag gcgggcgagg cggtcgaggg acgcggcctt cccgccgacg acgtccacgc
      121 cgtcgtccga tgacccgagc cagaccacgg tcgccgttgc aggaggcggc acccggcgtt
      181 ctccatcagc ggtgcgacgg tgtgcgcgac cgcgctcgac cgaagcgggt gcgagtctag
      241 cgcgccgttc acggcacgtt aacgcgcggg cgcacgatca tcgatcggtg aaggtgacca
      301 agggccctac cccccggcat ggcgcccggg ggacgatcaa cccagcgtcg atcctcccgg
      361 aggacagccg gtgcgaatcc ttctcgcgat ggacggctcg ccgtcatcta gccacgctcg
      421 cgatctggtg ggctcgcttt cgatcccgag cggatcgtcc gtgatggcgc tcatggcgta
      481 cgacatccca ggcacctggt ttggcgatct cctggctccc cgaggggagc cggctgaggc
      541 cgagaagagc gtttaccgcg gggccgtggc caccctggat cggttggccg aacccctgga
      601 cggccgcgac tggtcgatcg atcggcgcgt cgtccgtggc cgcacgccga acgcgattgt
      661 cgaggccgcc accgacctgg ccgccgatct catcgtggtc ggttcccgcg ggcacggccc
      721 gctcgcgtcg atgctgctcg gttcggtgtc tgccgaggtc gtcgagcggg cgacctgttc
      781 ggtcctggtc gctcgagggt caggcgtgtc ccgcctgctg gtggcaaccg acggctcacc
      841 gtgcgcagag ctcgttcccg aggtgctggc cacgtggggg gccttcaacg gcctgccgac
      901 gattgcactc agcgtcgcag aggccgattc ggccatcttt gggctgatgg tgggcatcta
      961 tacgatgggc agcctgtcgc ttgagcccga ccgccgggaa gtgcgcgaac gcgccggacg
     1021 gcacgcagcg gcgatggccg cacatctccg gttgattggc atccccgctc atccggaggt
     1081 tcggaccggg gacgtggcca gtgagatcat cgccgcggct gccgagcgtc aggccgacct
     1141 catcgtgacg gggtcgcgct cactgcatgg tcctgaccgg tggcttctcg gaagcgtcgc
     1201 gcgaaacgtg cttctgcact cgggagtctc ggtcctgatc gtgcggcgag gcgacgctgc
     1261 cgccggaccg tagccccccg ctcgccgtgg ccggccgacg tcggacccga cgtccggcgg
     1321 ccgacccact tctcgacctc gaccgccgca aagccggacg tggacgcgac caggacgacg
     1381 gccagctgcg gcatgctcag cgcgaccgta ccgaagatcg gctgcagggc cgggatgaag
     1441 atcagggcca actgcacgac gaacgtggcc gccaccgcga tcgtcaacgg catgttcgac
     1501 ctgagtccga gccggaacag gctctcggac tccgaacgga tggccagcgc atgcccgagc
     1561 tggaggaacg ccagggtggc gaagaccatc gtctgccacg gccagccctg gctgatggcg
     1621 tacgcctgga ccgcgagggc gactgacgcc atcagcagcc cgacccagat cgtgtgctgc
     1681 cagagcccgc gggcgaacag cgattcggac ggcgaccgcg gcggccgtcg catcgttccg
     1741 cgttctgtcg gctccacgcc gagggcgagg gccggcagcc catcggtcac gaggttgacc
     1801 cacaggatct gcacagcgag cagcgggaat ggaagaccaa ggaggggggc gagcgtcatc
     1861 acccagatct cggccgagtt cgttgtcagc agatagcgga cgaagcgccg gatgttgtcg
     1921 tagatccggc ggccttcctc gacggcggcg acgatcgtcg cgaagtcgtc gtcggcgagg
     1981 accatgtcgg cggcctcctt gctgacctcg gtcccggtga tgcccattgc tacgccgatg
     2041 tcggcccgcc gcagggccgg agcatcgttc accccgtcgc ccgtcatcgc gacgacggct
     2101 ccccggcgtc gccaggcgtc gacgatgcgg agcttctgtt ccgggttggt gcgcgcgtac
     2161 acggccacat cagccacgct cacatcgaag gcgacgttgt cgagctgctc gagctcggcg
     2221 ccggtcagac tgcgtccgcc gtcgcgcagg atgccgatcc gtcgggcgat ggcgttgccc
     2281 gtcagcggat gatcgccagt gatcatgatc ggcgtgatgc cggccgcacg gcaggccgcg
     2341 atcgacgggg ccgaggcctc gcggggcgga tccgccatcc cgacgatccc gagaagatga
     2401 aggccgtgct cggcgtcgtc gagccgggcg gggacgtcat cgatcgtgcg ctccgcgaac
     2461 gcgagcacgc ggtagccgtc gagggcgtag cgctcggcga cgtgctcggc ctgccgcagc
     2521 aagtcggcct catgtgcatc gagcagcggt ccgatcgcct ccagggcgcc cttcacggcg
     2581 acccagacat ccgcaccggc cctgtggatg gtcgccatcc gtcgtcgctc agggtcgaac
     2641 gtcagctcct cgacccgcgg ggatgcggca gcgaccgcgt ccggatccac gtcgagcttg
     2701 gcggcgaggg cgagcagtgc ggcctcggtc gggtcgccgc tgatggtcca cgggtcatcg
     2761 ctggtggccg gggcgtggag cgacgaatcg ttgcaggcgg cggcaacccg ggcgagtcgc
     2821 tccagggcag tgtcgttcgc cggctgggga tcgaaccgtc cggtcggtga gtagccgtcg
     2881 ccggttacgc gatagtcgcc gttcggggtc cagacccgtt cagccagcat ccggttctcg
     2941 gtcagggtgc cggtcttgtc gctgcagatc acggtgaccg accccagggt ctcgacggcc
     3001 ggcagcttgc gcacgagcgc gtggcgctgg gccatccgtc gggcgcccag ggccagggcg
     3061 atcgtgacga cggccggcag gccttccggg atcgcggcga ccgcgagact caccgcaacc
     3121 aggaacatca catcggccgg ctcgccgcgg gcgacgccgc tgacgaagac gaagatgcac
     3181 acgaggaacg ccgcgaacgc caatcgcttg ccgagcgtcg acagccgtcg ctggagcggc
     3241 gtctggccac tgccgtgctc ctcgaggagc tcggcaaccc gtccgagggc cgtctccatc
     3301 ccggtcgcca cgatcaggcc gaggccacgc ccataggtca ccgcggtgcc gctgaaggcc
     3361 atgttccgct ggtcgccgag gaccgccgcg gtgacattgg ccaacggggc cgtcaccttg
     3421 ctcgcgggct ccgattcgcc ggtcagggcg gcctcgttga ttcggagcga tcgagcctcg
     3481 agcaggcgca ggtcggcggt cacgacgtcc ccggcgtcga gccgcacaag gtcgcccggc
     3541 acgagctcgg tcgccggaac gacgatcgtg acgccggcac gaacgacgcg cgccgacggg
     3601 ctggtcatgc gcttcagcgc gtccatcgcc tgttccgccc tgtattcctg gacgaacccg
     3661 atgatcccgt tgagcgcgac gatgaccagg atgatggcgg tgtccttgag gtcgccgatg
     3721 gccgcggtga tcgcggcggc accgagcagg accaggatca tcgcgttggt gaactgttcg
     3781 gccagcaggc gccaggcgga cttggcggcg cgctccacaa gctcgttcgg tccgcacgcg
     3841 atgagacggt gtcgagcctc gtcgacgtcg aggccgcggg tcacatcaga gtcgagcgcg
     3901 gcgacgacct ctggagcatc gaccgcccat gggtccggga cgcggcgcag cgcggggacg
     3961 ccctcgacct ggacgcccga ggatccgccg acgcctggga gcacgctcac cttgctggtt
     4021 ccggttcgtc gcacctggcg tggtcgaaac gacgtcggcc ggtacgatcc cgagcgtcaa
     4081 cgccgcgaca cgcccggcgg aagtgccgat cggaccgatc ggcggcatcg gccatcccga
     4141 atcggacgga agtcagcgga cgttgagcgg aacaacgggg agagcaccat gggcaacgaa
     4201 caggtggcgc ccgagaccaa gggcgtggtg gtgagtgaac ttgcagcggt cgaccttggc
     4261 ggcgagatcg agggcatggc aggacgccag ctgcgaatgc ggatggtgac catcgagcct
     4321 ggtggcgtgt tcggcccgat ccacgaccat gcaggcaggc ccggcatcgt ctacgtcctg
     4381 cagggaacga tcaccgacca tcgagacgga gtcgcgaccg actatgggcc cggcatgggc
     4441 tggcccgagg atcggaacac gacccactgg ctcgagaacg ggggaacgat cccggcggtt
     4501 gagatctccg tcgacatcgt ccggcgggag tgagccgtca ccgccaacac gggcaaccgc
     4561 tgccccgagt cgcagtcgct ccatgcatgg gctgagcgca gccacgccgc caacgatgcc
     4621 gtcgccacgc ttgccggccc tgaacggcgc gtatcaccac gacgccagtc gctcgacgca
     4681 tgaaccgcgc gtcaacggcg acggatccac catgatggcc ctcggcgccc cgtcctgcgg
     4741 cgtccgctgt gcgtggtcga gaagtgcagc gactgcgggc ggcggcaaca ggcgggaccc
     4801 agcgtcgcac gcgccggcgt agccgaagca tctcgccgga tacagtgtct ccatgagcga
     4861 agtgaagatc ggcgcactgt gctggaacca gtacgcacca tggcccgccc tgcttcgggc
     4921 gggaaccctc gccgacgagt tgggctacga cacgctctgg acctgggacc acctctaccc
     4981 gatcgtgggc gactcgacgg ggc
//
LOCUS       DBNHXO010000294         2698 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2660725, whole genome shotgun sequence.
ACCESSION   DBNHXO010000294 DBNHXO010000000
VERSION     DBNHXO010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2660725"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="AC162A_06195"
     CDS             complement(<1..1141)
                     /locus_tag="AC162A_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HIR5010466.1"
                     /translation="MQLIDGRPVFAATDLVGFLACSHRLELERAALAGLVTKPIRNDP
                     SIELVAKRGIEHERRYLEDLREQGRRIVEIEQDGSAVAPLDAVGEPAPRDAGAELRAA
                     AAQTVAAMRGGADVVYQATFFDGTWRGHADFLLRRDHVPGEPDSAFGPWHYEVADTKL
                     ARHVKASAILQICSYVEQLTAVQGLQPEYLYVVLGGRERPTDQLRVDDYMAYYRRVKD
                     EFEASVGVRGDTAAPVAYPPVGTYPEPVEHCDVCRWAVMCKARRRADDDLSLVAGAGS
                     RPRRALKARGIPTRRGLAGLELPMAPSLDGVGDAALERIREQARIQVESEDRGEVLWE
                     LLPLDRDKDGVPVPDRGLLVLPEPSEGDLFLDLEGDPFALDDGVDY"
     gene            complement(1181..2482)
                     /locus_tag="AC162A_06200"
     CDS             complement(1181..2482)
                     /locus_tag="AC162A_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010467.1"
                     /translation="MAVTIKVVGQAAKRGLIPRIPAPDKLIEDARAWVAAEYPQHVRS
                     ARVLSAPTGTELWLDLHPAAEPVTVTADEEGRVTGSADTGDGGPGYHTYVGRVFDRLG
                     EQLDIVWSHEHDPRAIGAPAPWIGAKQPVAERAAVEADHLAYLGQVVDRAVGQRRLGI
                     ASVGIGLRTGTGFQIDGAVGTPLGPRDDAWLGRAAKNPWAATDIRPWWFDVMDARYLL
                     QRAMVILWTEIRWRSPVDDEERTVVDEVLSLLRRALPMDASLAYPWREWAELLRLRGA
                     PDPIRDRVFRQAEQMDPALPLVGYRRLPVTIVHEGWTFQVPGTFDERVADGEWHGGER
                     GRRVTFAATVTQAANGMPMSAERFLTEVAGDIVEGVLRHEDGAIQGRARVTSDSSSGL
                     EVAVLEGFSAVTGSGAAMRIEFDRTEDWQWALDLWRSVRPV"
BASE COUNT          358 a         1016 c          961 g          363 t
ORIGIN      
        1 ggtagtcgac gccgtcgtcg agcgcgaacg ggtcgccctc gaggtcgagg aacaggtcgc
       61 cctcgctggg ttccgggagc acgagcagcc cgcggtccgg caccgggacc ccgtccttgt
      121 cgcgatccag cggcagcagc tcccacagca cctcgccccg atcctcggat tcgacctgga
      181 tccgcgcctg ctcgcggatc ctctccagcg cggcatcgcc gacgccgtcg agggagggcg
      241 ccatcgggag ctcgagtccg gcgagcccgc gccgcgtcgg gatgccgcga gccttgaggg
      301 cgcgtcgcgg ccggctgccc gcccccgcga cgaggctcag gtcgtcgtca gcacgtcggc
      361 gcgccttgca catcacggcc cagcggcaga cgtcgcagtg ctcgacgggc tcggggtagg
      421 tgccgaccgg cgggtaggcg accggagccg ccgtgtcgcc gcgcaccccg acggatgcct
      481 cgaactcgtc cttgacccgg cggtagtagg ccatgtagtc gtcgacccgg agctggtccg
      541 tgggtcgctc gcggccgccc aggacgacgt acaggtactc gggctggagg ccctggaccg
      601 ccgtcagctg ctcgacgtac gagcagatct ggaggatcgc ggacgccttg acgtggcgcg
      661 cgagcttggt gtcggcgacc tcgtagtgcc aggggccgaa ggcggagtcc ggctcgcccg
      721 ggacgtggtc gcggcggagc aggaagtccg cgtggccgcg ccaggttccg tcgaagaacg
      781 tcgcctggta gacgacgtcc gcaccgccac gcatcgccgc gaccgtctgc gcggccgccg
      841 cccggagctc ggcgccggcg tcgcgcggcg cgggctcgcc cacggcatcc agcggcgcga
      901 cggccgatcc gtcctgctcg atctcgacga tccgccgccc ctgctcgcgc aggtcctcga
      961 gataccggcg ctcgtgctcg atgccgcgct tggcgacgag ctcgatcgaa gggtcgttgc
     1021 ggatcggctt ggtcacgagc ccggcgaggg cggcgcgctc cagctcgagc cggtggctac
     1081 aggcgaggaa cccgacgaga tcggtggcgg cgaagacggg gcgtccgtcg atcagttgca
     1141 tgcgggccca tcctacgggc cacccgtgtc agctcgcgtg tcacaccggc cggacggacc
     1201 gccacaggtc gagcgcccac tgccagtcct cggtgcggtc gaactcgatg cgcatcgccg
     1261 ccccgctgcc ggtcaccgcg gagaagccct cgagcacggc gacctcgagc cccgagctgg
     1321 agtccgaggt gacccgcgcg cggccctgga tcgcgccatc ctcgtgccgc agcacgccct
     1381 cgacgatgtc gccggcgacc tcggtcagga accgctcggc cgacatcggc atcccgttcg
     1441 ccgcctgggt cacggtcgcg gcgaaggtca cccggcgccc gcgctcgccg ccgtgccact
     1501 ccccgtcggc gacccgctcg tcgaacgtgc cgggcacctg gaaggtccag ccctcgtgca
     1561 cgatcgttac cggcaggcgt cggtagccga cgagcggcag ggccgggtcc atctgctcgg
     1621 cctggcggaa cacgcggtcc cggatcgggt cgggcgcgcc gcgcagccgg agcagctccg
     1681 cccactcgcg ccacgggtag gcgagcgacg cgtccatcgg cagcgcgcga cggagcagcg
     1741 agagcacctc gtcgaccacc gtccgctcct cgtcgtcgac cggggaccgc cagcggatct
     1801 cggtccacag gatcaccatg gcgcgctgga gcaggtagcg cgcgtccatc acgtcgaacc
     1861 accagggccg gatgtccgtc gccgcccagg ggttcttggc ggcacgtccg agccaggcat
     1921 cgtcgcgcgg cccgagcggc gtgccgacgg ccccgtcgat ctggaatccc gtccccgttc
     1981 gcagcccgat cccgacgctg gcgatgccga ggcgtcgctg cccgacggcg cggtccacga
     2041 cctgcccaag gtacgcgagg tggtctgctt cgacggccgc acgctccgcg acgggctgct
     2101 tggcgccgat ccacggggcc ggtgcgccga tcgcgcgcgg gtcgtgctcg tgggaccaga
     2161 cgatgtcgag ctgctccccg aggcggtcga acacccggcc cacgtaggtg tggtagccgg
     2221 ggccgccgtc gcccgtgtcg gccgaaccgg tgacgcgtcc ctcctcgtcc gccgtgaccg
     2281 tcacgggttc ggcggcgggg tgaaggtcga gccaaagctc ggtaccggtc ggcgccgaca
     2341 ggacgcgcgc ggaccggacg tgctgggggt actccgcggc gacccacgcc cgggcgtcct
     2401 cgatcagctt gtccggcgcc ggtatccgtg ggatcaggcc gcgcttcgct gcctggccca
     2461 cgaccttgat cgtgactgcc aatcccgctc cctcggacca gcccccggta accccgccga
     2521 ccatagccaa cgccggaccc ggatggcgag ccctgggcac accttttcgt acttcggcgg
     2581 gcagccgacg gtcccgtccc gctatcgttc gagcgatgcc gcagcccgag cgccgccccg
     2641 catcgcgcct cgacgccgag ggcaaccgac aggtcgcccc gacgtgggag agcctcgt
//
LOCUS       DBNHXO010000295         8232 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2660848, whole genome shotgun sequence.
ACCESSION   DBNHXO010000295 DBNHXO010000000
VERSION     DBNHXO010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 8232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8232
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2660848"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="AC162A_06205"
     CDS             <1..365
                     /locus_tag="AC162A_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010468.1"
                     /translation="FTSDALPVHLITAEALADAQRTLRPGGLMAIHVSNRYYDLEPPV
                     SAAMEAAGFNVVRRVYTPTADEVEAGATVSHWLIGGRPGESDADLGALTARGWSSPRV
                     GEPLTDDFADLLRYLRLR"
     gene            complement(787..1176)
                     /locus_tag="AC162A_06210"
     CDS             complement(787..1176)
                     /locus_tag="AC162A_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014678.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HIR5010469.1"
                     /translation="MATNAATGVARNVERSTVLAERLETAGSLWAKFMGLMGRRSLPP
                     GDGLWLPASNGIHMMFMRFPIDAVFVTKADAAGVRRVRSLHPSLAAWTGMVPLIRGAD
                     GVLELPIGTIAASGTVVGDRLEIVERA"
     gene            complement(1188..2123)
                     /locus_tag="AC162A_06215"
     CDS             complement(1188..2123)
                     /locus_tag="AC162A_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR5010470.1"
                     /translation="MDNLLPLLIAGVAAIAILLIFVGLAGNSPVDPVHARLTQLGTMQ
                     AKNLEELELQQPVFERTIRPLANRLSGATSRVASTSFTATTEKRLALAGNPGDLRTAD
                     WLGIKAVGAIVGGLIFFVLFFIVGVLGLPLVLRAVMIPIGMMFGYTLPEFWLGGRVRK
                     RQKAILLQIPDALDLLTISVRAGLGFDGALGKVVEKLKGPLTEEFRRALAEIRVGKAR
                     RDALRDIVPRTEVPALTNFIGAIIQAEQLGVSISKVLQVQSEQLRIERRQRAEEMAAK
                     APIKMLFPLVGCIFPSLFIVILGPAIILIMINLKT"
     gene            complement(2129..3142)
                     /locus_tag="AC162A_06220"
     CDS             complement(2129..3142)
                     /locus_tag="AC162A_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR5010471.1"
                     /translation="MDMTIVIAGIAALAILTIAVGIAMTGGGSGVNARLERYASTKPD
                     KPATGATGTAGFAEAFQSSAALAQLNKVVEQRDFGANLAREIARADLKLKVSEYLVIW
                     AGCVFAVPLLMLLLSIVFPGLGNPIVLLVGALIGFMLPRFWLNRRKSGRLNSFNKQLP
                     DTITLIANALRAGSSFLQAIELVVRESRPPISTEFGRVIREVNLGLSFEVALENMVRR
                     VKSDDLELMATAISIQHTVGGNLAEILDSIAFTIRERVRIKGEIKTLTAQQRLSGYVV
                     GFLPIGLCLFLFVAAPSFMSPMFDSRVSLIGLPGGVIILAIGGFAMFIGFMLIRKIVD
                     IEV"
     gene            complement(3169..4575)
                     /locus_tag="AC162A_06225"
     CDS             complement(3169..4575)
                     /locus_tag="AC162A_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HIR5010472.1"
                     /translation="MSLLKRIESARPGTGTTPEGSNVPVPTPGGGGTTTPAAPQGRLM
                     TQAPVRESFRDVKFRIQSRVIQDLDPKLDLSNQVEVRRQIEEIFGKVIDEEGLALTRA
                     ERVRMLEQITDEIIGLGPLEPLLRDESISEVMVNGPRQVYIERSGRLELTNVVFQNDD
                     HVMRIIDRIIAPIGRRVDESSPMVDARLTDGSRVNAIIPPLSLVGPVITIRKFSASPF
                     TVDDLIRFGTATPDMFEFLRASVEARLNLFVSGGTGSGKTTTLNVLSSFIPNDERIVT
                     IEDAAELQLRQEHVVTLESRPANIEGKGQVPIRELVRNSLRMRPDRIIVGECRSGEAL
                     DMLQAMNTGHDGSMSTGHANSPRDMLARLETMVLMAGVDLPVRAIREQIASAVDLIVH
                     QSRLKDGTRRITNITEVQGMEGDVIVMQDVFVFEQTGVVEGKIQGRLKPTGIRPKFVE
                     KFEVQGIHLPPGLFGSTY"
     gene            complement(4623..5903)
                     /locus_tag="AC162A_06230"
     CDS             complement(4623..5903)
                     /locus_tag="AC162A_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE/TadZ family protein"
                     /protein_id="HIR5010473.1"
                     /translation="MADQIRVLIVDDIPETRDHLSKLLGFESDIEVVGSAASGREALK
                     MAAELTPDVVLMDINMPDMDGISATERLSAEVPAAAVVMMSVQGEADYLRRSMLAGAR
                     EFLVKPFSSDELTSSIRQVYTREREKMSRMAVVPQPAPAAGGGAAAPARPASSTREAG
                     EPGRVVAVFGPKGGVGRTTLAVNLAVAAATELGQTTCLVDASFQFGDIGVLLNLNPKN
                     KSIADVIPELEHGDIESLDPFVINHSAGIKVLLAPPSPEMAELITPAGAKRMIEALRS
                     ANDLVVVDCMSTFNDTTLAILDLADTVLTMLSLEITSIKNIRLFLEVADQLGYGSDKI
                     RLVLNRADSSLGIRVADVEHSIGRRVDHTIVSDGRSVVYALNRGVPFFLSNREAQVSQ
                     DVLRLASAVVGSKAEAADEPSRKAGQKKSLFAWR"
     gene            complement(6014..7048)
                     /gene="cpaB"
                     /locus_tag="AC162A_06235"
     CDS             complement(6014..7048)
                     /gene="cpaB"
                     /locus_tag="AC162A_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HIR5010474.1"
                     /translation="MKRSNRLVLLIGIFLALIAFVLILMTLGGGGGPGGPTETTPTTT
                     TIVVASRDIELGDTIKEADVEESEINITDFPPDGIKLESLVIGQISRANVVKGQAVTT
                     EVLEGASGTIGNVTVPPGFVAMAVQVDQVTGVGTVIKAGDYVDMVTGITGMDKVPVVV
                     SPTSRPTNATPRPNATPPPRAGFIPEPLPYNSTTVKTLIQGVQVLGTLLPPTEQNTDN
                     QTPPPGSEANTTTLNGQQQIVILAVTPQDAEIIKFAQIDGTISLLLRATADCQPSESA
                     SPSPSPSASAEPGASPSASPLINGNCPIRFTTGITLRRLVDDRGVLPPQIVEVIQPTP
                     YPQVPASPAP"
     gene            complement(7345..7542)
                     /locus_tag="AC162A_06240"
     CDS             complement(7345..7542)
                     /locus_tag="AC162A_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HIR5010475.1"
                     /translation="MIVLHTRAFIQRLTDPDDENGQGLAEYALILALIAIVAIIALIF
                     LGNQVSDKFSVIGDAIDSVTP"
     gene            complement(7698..7868)
                     /locus_tag="AC162A_06245"
     CDS             complement(7698..7868)
                     /locus_tag="AC162A_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HIR5010476.1"
                     /translation="MTLFNALIGLINRDEEGQGLAEYALILALIAIVAIVALIFLGNQ
                     VSQILSTVGKSI"
BASE COUNT         1340 a         2651 c         2907 g         1334 t
ORIGIN      
        1 ccttcacgtc cgacgcgctg ccggtccacc tgattacggc ggaggccctc gccgacgccc
       61 agcgcacgct gcgtccgggc ggcctgatgg ccatccacgt ctcgaaccgc tactacgacc
      121 tcgagccacc ggtctcggct gccatggagg ccgccgggtt caacgtcgtc cggcgcgtct
      181 atacccccac ggccgatgag gtcgaggcgg gggcgacggt ctcgcattgg ctcatcggcg
      241 gccggccagg cgagtccgac gccgatctcg gggcgctcac ggcccgcggc tggtcatcgc
      301 cccgcgtcgg cgagcccctc accgacgact tcgccgacct gctgcggtac ctgcgcctgc
      361 ggtaggaccg gcgggtctcc gcctcttgac cggatctcgc gcccgggatc tcgcgcccga
      421 accggtcatc catgccccac atgaatgcaa gccgcgccca tcgttgccag atatgcccgt
      481 ggcgactacg aagcctcgaa atgggacgcg cacttggcca tccattcacc atgtgagtcg
      541 tcggccatgt atcgccgtcc ggaccgagcc aagcattcgc cccgcgaata tctggcatgg
      601 tccgcgcgtg gcgccacgct cagccgcgtt cgccgcgcat cgcgggcgtg gccaagtgtg
      661 ggcccggcat tcccctcatg aatgcatggc cgactcgccg cgcacggaac gccgcgcggg
      721 ccggggtcgg cgagggccgg ggtcggcgag ggccggggag atcgctggcg ggatgacccg
      781 cggcggctag gccctctcga cgatctccag gcgatccccg acgaccgtgc cgctggcggc
      841 gatcgtcccg atgggcagct ccaggacccc gtcggcgccc cggatcagag gcaccatgcc
      901 ggtccacgcg gccagcgacg ggtgcaggga ccggacgcgc cgcacgcccg cggcatcggc
      961 cttggtcacg aacaccgcgt cgatcgggaa gcgcatgaac atcatgtgga tcccgttgct
     1021 cgccggcagc cacaggccgt cgccgggcgg cagcgagcgc cgccccatga ggcccatgaa
     1081 cttggcccac agcgagccgg ccgtctccag ccgctcggcg agcacggtcg accgctcgac
     1141 gttgcgcgcg accccggtgg cggcgttggt cgccatcgga tcggtggcta ggtcttgagg
     1201 ttgatcatga tcaggatgat cgccggcccg aggatgacga tgaacagcga cgggaagatg
     1261 cacccgacga gcgggaacag catcttgatc ggcgccttgg ccgccatctc ctcggcgcgc
     1321 tgccggcgct cgatccgcag ctgctccgac tgaacctgga gcaccttgct gatcgacacg
     1381 ccgagctgct cggcctggat gatcgccccg atgaagttgg tgagcgccgg gacctcggtc
     1441 cggggcacga tgtcccgcag cgcgtcgcgg cgggccttgc cgacccggat ctccgccagc
     1501 gcccggcgga actcctcggt gaggggtccc ttgagcttct cgacgacctt gcccagcgcc
     1561 ccgtcgaagc cgaggcccgc gcggaccgag atggtcagca ggtcgagggc atccgggatc
     1621 tggagcagga tcgccttctg gcgcttgcgg acgcggccgc cgagccagaa ctcgggcagc
     1681 gtatagccga acatcatccc gatcgggatc atgaccgccc gcagcacgag cggcaggccg
     1741 agcacgccga cgatgaagaa caggacgaag aagatgagtc cgccgacgat ggcgcccacg
     1801 gccttgatgc ccagccagtc ggccgtgcgc aggtcgcccg ggttaccggc gagcgcgagg
     1861 cgcttctcgg tcgtggccgt gaacgacgtg ctcgcgacgc gcgacgtggc gccggacagg
     1921 cggttggcga gcgggcggat cgtccgctcg aagacgggct gctggagttc gagctcctcg
     1981 aggttcttgg cctgcatcgt gcccagctgg gtcaaccgcg cgtggaccgg gtccacgggc
     2041 gagtttccgg ccaggccgac gaagatcagg aggatcgcga ttgccgcgac ccccgcgatg
     2101 agcaggggca gaaggttatc catcggcgct agacctcgat gtccacgatc ttgcggatca
     2161 acatgaagcc aatgaacatc gcgaacccgc caatcgcgag gatgatcacg ccgcccggga
     2221 gtccgatcag ggacacgcgg ctgtcgaaca tcggcgacat gaaggacggt gcggcgacga
     2281 acaggaacag gcacaggccg atcggcagga agccgacgac gtagcccgac agtcgctgct
     2341 gcgcggtaag ggtcttgatc tcgcccttga tccggacccg ctcacggatc gtgaacgcga
     2401 tcgagtcgag gatctcggcg aggttaccgc caacggtgtg ctggatcgag atggcggtcg
     2461 ccatcagctc gaggtcgtcg gacttgaccc ggcggaccat gttctcgagc gcgacctcga
     2521 acgacaggcc gaggttgacc tcgcggatga cgcggccgaa ctcggtcgag atgggcggcc
     2581 gcgactcgcg gacgacgagc tcgatcgcct ggaggaacga ggagccggcc cgcagggcgt
     2641 tggcgatcag ggtgatggtg tcggggagct gcttgttgaa cgagttgagc cggccggact
     2701 tgcggcggtt cagccagaac cgcggcagca tgaacccgat gagcgcgccc accaggagca
     2761 cgatcgggtt gccgagcccg gggaacacga tcgacaggag cagcatcaac aggggcactg
     2821 cgaagacgca gccggcccag atcacgaggt actcgctgac cttgagcttc aggtccgcac
     2881 gcgcgatctc gcgggccagg ttggccccga agtcgcgctg ctcgacgacc ttgttcagct
     2941 gcgcgagcgc cgcgctggac tggaaggcct cggcgaagcc ggccgtcccg gtcgctccgg
     3001 tcgcgggctt gtccggcttg gtcgacgcgt agcgctcgag gcgcgcgttg acgcccgagc
     3061 cgccgccggt catggcgatg ccaacggcga tcgtcaggat cgcgagggca gcgatgccgg
     3121 cgatgacgat ggtcatgtcc atgggtgcag tcctctcgtg ccggcgatct agtaggtgga
     3181 gccgaacagg cccggcggca ggtgaatgcc ctggacctcg aacttctcga cgaacttggg
     3241 ccggatgccg gtcggcttca ggcggccctg gatcttgcct tcgacgacgc ccgtctgctc
     3301 gaacacgaac acatcctgca tcacgatgac gtcaccttcc atcccctgga cctcggtgat
     3361 gttcgtgatc cggcgcgtgc cgtccttgag gcgggactgg tggacgatga ggtcgaccgc
     3421 cgacgcgatc tgctcgcgga tggcgcggac cggcaggtcg acgcccgcca tgagcaccat
     3481 cgtctcgagc cgcgcgagca tgtcgcgggg gctgttcgcg tggcccgtgg acatcgagcc
     3541 gtcgtggccg gtgttcatgg cctggagcat gtccagcgcc tcacccgaac ggcactcgcc
     3601 gacgatgatg cggtcgggcc gcatgcgcag cgagttccgg acgagctccc ggatcgggac
     3661 ctggcccttg ccctcgatgt tggcggggcg ggactcgagc gtcaccacgt gctcctggcg
     3721 gagctgcagc tcggcggcgt cctcgatcgt gacgatccgc tcgtcgttcg ggatgaacga
     3781 cgacaggacg ttgagcgtcg ttgtcttgcc ggagcccgtg ccgccgctga cgaacaggtt
     3841 gaggcgcgcc tcaaccgacg cccgcaggaa ctcgaacatg tccggggtcg cggtgccgaa
     3901 ccggatgagg tcgtcgaccg tgaaggggga ggccgagaac ttccggatcg tgatgaccgg
     3961 cccaacgagg gacaggggcg ggatgatggc gttcacgcgg gagccgtcgg tcagtcgggc
     4021 gtcgaccatg ggcgacgact cgtcaacgcg gcggccgatc ggcgcgatga tccgatcgat
     4081 gatgcgcatg acgtggtcgt cgttctggaa gacgacgttc gtgagctcga ggcggccgct
     4141 gcgctcgatg tagacctggc gcgggccgtt gaccatgact tccgagatcg actcgtcgcg
     4201 caggagcggt tcgagcggcc cgaggccgat gatctcgtcg gtgatctgct ccagcatccg
     4261 gacgcgctcg gcgcgcgtca gggcgaggcc ttcctcgtcg atgaccttgc cgaagatctc
     4321 ctcgatctgc cggcgcacct cgacctggtt ggacaggtcg agcttcgggt ccaggtcctg
     4381 gatgacgcgg ctctggatcc ggaacttgac gtcccggaac gactcgcgca cgggtgcctg
     4441 agtcatcagg cggccctgcg gcgcagccgg cgtcgtcgtc ccgccgccac ccggcgtcgg
     4501 gacgggaacg ttggacccct cgggcgtcgt gcccgtgccg ggtcgagcgc tttcgatccg
     4561 cttcaagagg gacatcggtt acaccccttc ggccgtgagc ggccattgtg cgcgctgtcg
     4621 catcatcgcc atgcgaacag cgacttcttc tgaccggcct tgcggctggg ctcgtcggcg
     4681 gcttccgcct ttgagccaac cacggccgag gcgaggcgca gcacgtcctg tgagacctgc
     4741 gcctctcggt tggagaggaa gaatggcacc ccccggttga gggcgtagac gacgctccga
     4801 ccatcggaga cgatcgtgtg gtcgacacgc cgcccgatcg agtgctccac gtcggcgacg
     4861 cggatgccga gcgacgaatc ggcgcgattg aggacgagcc ggatcttgtc cgacccgtag
     4921 ccgagctgat ccgccacctc gaggaagagg cggatgttct tgatcgacgt gatctcgagc
     4981 gagagcatcg tgagcacggt atccgcaaga tcgagaatcg caagggtcgt gtcgttaaag
     5041 gtcgacatgc agtcaacgac caccaggtcg ttggcgctcc gcagcgcctc gatcatgcgc
     5101 ttggcgccgg ccggtgtgat cagctcggcc atctccggtg acggaggggc gagcaggacc
     5161 ttgatgccgg ccgagtggtt gatcacgaac gggtcgagcg actcgatgtc cccgtgctcg
     5221 agctcgggga tgacgtccgc gatggacttg ttcttggggt tcaggttgag caggaccccg
     5281 atgtcgccga actggaacga ggcgtcgacg agacaggtcg tctggccgag ctcggtcgcc
     5341 gcggcgacgg cgaggttgac ggcgagcgtg gtgcgtccga cgcccccctt gggcccgaac
     5401 accgcgacca cccggcccgg ttccccggcc tcgcgggtgg acgatgcggg tcgggcgggg
     5461 gcggccgccc caccgccggc agctggtgcc ggctgcggga ccaccgccat gcgcgacatc
     5521 ttctcgcgct cccgggtgta gacctgccgg atcgacgagg tcagctcatc cgaggagaac
     5581 ggcttgacga ggaactcgcg tgccccggca agcatcgagc ggcggaggta gtcggcctcg
     5641 ccctggacgg acatcatcac gaccgcggcg gccggcactt cggccgacag acgctccgtc
     5701 gccgagatgc cgtccatatc gggcatgttg atgtccatga gcacgacgtc gggcgtcagc
     5761 tcggcggcca tcttgagcgc ctcgcgtccg gacgccgcgg atccgacgac ctcgatgtcc
     5821 gactcgaagc ccaggagctt cgagaggtgg tcgcgggtct ccgggatgtc gtcgacgatc
     5881 aggacacgga tctggtctgc catgggtctc ggatacgggt tgccgttctg gggtggatcg
     5941 gacgtcgatg ggccggtgga gaccggccca tcgggtcgct ggagcgggcc gctggcccgc
     6001 ggtggaggcg gtgttacgga gccgggctag ccggcacctg cgggtacggg gtcggctgga
     6061 tgacttcgac gatctgcggc gggagcacgc cccggtcgtc gacgagacga cgcagcgtga
     6121 tgccggtggt gaacctgatc gggcagttgc cattgatcag cgggctggcg ctcgggctcg
     6181 cacccggctc agcgctggcc gacggcgacg gcgacgggct ggcgctctcg gacggctggc
     6241 agtcagcggt tgcccgcagc agcagcgaga tggtcccgtc gatctgggcg aacttgatga
     6301 tctcggcgtc ctgcggggtg accgcgagga tgacgatctg ctgctggccg ttcagcgtgg
     6361 tcgtgttggc ctccgagcca ggaggcggcg tctggttgtc ggtgttctgc tcggtcggcg
     6421 gcagcagcgt gccgaggacc tggacgccct ggatgagcgt cttgacggtg gtcgagttgt
     6481 agggcagcgg ctccgggatg aacccggcgc gcggcggggg cgtggcgttg ggtcgcggcg
     6541 tggcgttggt gggtcgcgag gtcggcgaga ccacgaccgg caccttgtcc atgcccgtga
     6601 tgcccgtgac catgtcgacg tagtcgccgg ccttgatgac cgtgccgacg ccggtgacct
     6661 ggtcgacctg gacggccatc gccacgaagc ccgggggaac cgtcacgttc ccgatggtgc
     6721 ccgaggcgcc ctcgaggact tccgtcgtca cggcctggcc cttgaccacg ttggcgcggc
     6781 tgatctggcc gatgacgagg ctctcgagct tgatcccgtc aggcgggaag tccgtgatgt
     6841 tgatctcgga ctcttccacg tctgcttcct tgatcgtgtc gccaagctcg atgtcccgcg
     6901 acgcaacgac gatcgtcgtg gtcgtgggcg tcgtttcagt cgggccgcct ggcccgccac
     6961 ccccgcctag cgtcatgagg atgaggacga acgcgataag tgcgaggaag atcccgataa
     7021 gcaggaccaa ccggttcgaa cgcttcaact ggacctcccg agccgtgtga caggtggcac
     7081 cactctaaca tcgtgattca gggcgaacac cagccaaaag gcctaggacc attggaagga
     7141 aatgggtaga tgggccgcac accgtaaggt ggcctgcaag gcactgagca cgcccgcgag
     7201 tgtgccggac gggcgctccc aggcttcagc gctgagccaa cgagcgtgaa tcgacgatcg
     7261 tgggctcagc gagctggaca tggaacggcc cgccggcggt tgccgacggg ccatgttcgg
     7321 tgcgactgac aattgaagcg cccgtcaggg cgtcaccgag tcgatcgcgt cgccgatcac
     7381 actgaacttg tcgctgacct ggttaccaag aaagatgaga gcgatgatgg cgacgatggc
     7441 gatgagggcg aggatgagtg cgtattccgc gagaccctgg ccgttttcgt catccggatc
     7501 ggtgagccgc tggatgaacg ctctggtgtg cagaacgatc attccggtac tcacctctct
     7561 gtggttgggg ctgttgctcg tcccggttgg gccaggaggc ccgccgttga cgatcgtcca
     7621 tcacagtccc tggaggcgtt gcgccggccc ctagtcgaga ccggcgcgtc gcaatcagtg
     7681 actgtcgacc accggggtca gatggacttg ccgaccgtcg agaggatctg cgagacctgg
     7741 ttgcccagga agatgagggc gacgatcgcg acgatcgcga tcagggccag gatcagcgcg
     7801 tactcggcga gaccctgacc ttcctcgtcc cgattgataa gaccaatcag tgcattgaac
     7861 agcgtcatgg gatggattac ctcctttcct caaaagctcg cgaacccgtc gcggcgaggg
     7921 cgaacgatat gtggactcca gcgagggcgt caacccggac catctggcca cgttgcacgc
     7981 ctgcaccccc gtgggtgccc tggatgcacg aaggccgccg aacaacgccg gcggccttca
     8041 ctctgcgtaa cgggtggcag ctagatggac ttgccgaccg tcgagaggat ctgcgagacc
     8101 tggttgccca ggaagatgag ggcgacgatc gcgacgatcg cgatgagggc gaggatcagc
     8161 gcgtactcgg cgagtccctg gccctcctcg tcccgctcga gcgaggcgag gaacgaggtg
     8221 atgagcgcca ta
//
LOCUS       DBNHXO010000296         3994 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2066441, whole genome shotgun sequence.
ACCESSION   DBNHXO010000296 DBNHXO010000000
VERSION     DBNHXO010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2066441"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="AC162A_06250"
     CDS             <1..513
                     /locus_tag="AC162A_06250"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012497.7"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR5010477.1"
                     /translation="PCEHVEALVELFGRVGVPVACGKSSIEIDATGLRAGDLRACDIE
                     TEPYPGLATDLQPPACVLLTQASGTSRIHEAIFEDRLEWLAELAKLGAQVEIADNHHA
                     LITGPSKLRGAETDISDLRAGASLILAALAAEGTSLIHGAHHVQRGYENIDRKFQQLG
                     ASITRSADTA"
     gene            604..1602
                     /gene="mreB"
                     /locus_tag="AC162A_06255"
     CDS             604..1602
                     /gene="mreB"
                     /locus_tag="AC162A_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HIR5010478.1"
                     /translation="MKIGIDLGTANVIVYVKGKGIVITEPSVVAVGEDNRIVAVGEEA
                     REMIGRTPGNIQAIRPMKDGVIADYVITEAMLRFFIGKATKGRIGFSKPEVMISVPAG
                     VTSVEKRAVRDAALKAGAKEAFLIEEPLAAAIGANVPISGPSGNMIIDIGGGTSEIAV
                     IALGGIVVSNSLRVGGNKFDEAIATYIRKKYNLMVGERTAEEVKIAIGAALPLERELS
                     MEVRGRDLIAGLPRTIPITSSEVMEAIEVPLQQLVAAVRTVLEQTPPELSSDIIDKGM
                     VMSGGGSLLRNIDKLLTQVTGVPCHVAENSLNCVALGTGLALEHFDFFKKSLVQQI"
     gene            2000..2710
                     /locus_tag="AC162A_06260"
     CDS             2000..2710
                     /locus_tag="AC162A_06260"
                     /inference="COORDINATES: protein motif:HMM:NF014826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR5010479.1"
                     /translation="MRAFVDLHCHTSVSFDSLASPEAVVRAAAKRGLTHLAITDHDRI
                     EGALRARDAAPEGLTVIIGEEVKTADGDLIAVFLERAVAPGRPVRETIAEVREQGGLV
                     GIPHPFDRFRGSMLKDPRLEAMAPLVDWVEAHNARVVGGSGNERAAAFAHEMGLPGIA
                     VSDAHNVVEVGVAYIAVDGDPSTPEGLMAALTNVELVPGRASYIVRTLTPLAKLVNRA
                     RGNRRVPISPAGDAGAST"
     gene            2707..3852
                     /locus_tag="AC162A_06265"
     CDS             2707..3852
                     /locus_tag="AC162A_06265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR5010480.1"
                     /translation="MSEPPVTDADATSPANPLARQGGSTRVPRTGSASDGNGGDGGQI
                     SLGERLRQPRTIISLGVPLVLLVLIFRVALNVDFAELANSVASANKLLLVAALLVFYA
                     GFPIRGMRWALLLKGAGMRVGTKDATEILFLSWTVNCLVPAKLGDLYRAFLLKINADV
                     SGSRTLGTIFIERVFDLFAIAIMGLLAGFWSFRTGFPPEIQFVAVLGIGVIVVLAGFL
                     FAVRNFGRAILGKLPLPDRALEFYDRFEEGLFSINRRSLGPLAILTVLIWVTEGMRLW
                     LVIQALGLPGVSMGISGAFFVAFIGSLLTAVPLSPAGLGTVELGVVGVLTLAYGLPTQ
                     EATTIVLVDRLISVFSIILIGSIVYLVSPIRRGAGLSQPVEASAPAG"
BASE COUNT          593 a         1400 c         1374 g          627 t
ORIGIN      
        1 ccctgcgagc atgtcgaggc cctggtcgag ctgttcggcc gggtcggcgt tcccgtggcc
       61 tgcggcaagt cctcgatcga gatcgacgcg acgggcctgc gcgcgggcga cctccgggcg
      121 tgcgacatcg aaaccgagcc ctatccgggg ctcgcgaccg acctccagcc cccggcatgc
      181 gtcctgctga cacaggcgtc cggcacgtca cggatccacg aggcgatctt cgaggaccgg
      241 ctcgagtggc tcgccgagct cgccaagctc ggggcgcagg tggagatcgc agacaaccac
      301 cacgcgctga tcaccgggcc ctccaagctg cgcggggccg agaccgacat cagcgacctg
      361 cgtgccgggg catcgctcat cctggcggcg ctggcggccg agggcacctc gctgattcac
      421 ggcgcgcatc acgttcaacg cgggtatgag aacatcgacc gcaagttcca gcagctgggt
      481 gcgagcatca cccgctcggc cgatacggcc tgaccaccgc ccggccgccc acccgtccgc
      541 accactcgcg ccgaacgccc aaccctgggc gcggcgccca cctggggtag cattcaccgg
      601 cccatgaaga ttggaatcga cctcggtacc gcgaacgtca tcgtgtacgt caagggcaag
      661 ggcatcgtga tcacggagcc ctccgtcgtc gcagtcggcg aggacaaccg gatcgtcgcc
      721 gtgggcgaag aggcccgcga gatgatcggc cggactcccg gcaacatcca ggccatccgc
      781 ccgatgaagg acggcgtcat cgccgactac gtcatcacgg aggcgatgct gcggttcttc
      841 atcggcaagg cgaccaaggg ccgcatcggc ttttccaagc ccgaggtgat gatcagcgtg
      901 cccgccggcg tgacgtcggt cgagaagcgc gcggttcgcg acgcggccct caaggcgggc
      961 gcgaaggagg cgttcctgat cgaggagccc ctcgcggcgg ccatcggcgc aaatgtcccg
     1021 atcagcgggc cgtcggggaa catgatcatc gacatcggcg gcggcacgtc cgagatcgcg
     1081 gtcatcgcgc tcggcgggat cgtggtcagc aacagcctcc gggtcggcgg caacaagttc
     1141 gacgaggcga tcgccaccta catccgcaag aagtacaacc tgatggtggg tgagcggacg
     1201 gcggaggagg tcaagatcgc gatcggggcc gccctgccgc tggagcgcga gctgagcatg
     1261 gaggtccgcg gccgcgacct gatcgccggg ctgccgcgca cgatcccgat cacgtcgtcc
     1321 gaggtgatgg aggcgatcga ggtcccgctc cagcagctgg tcgccgcggt ccgcacggtg
     1381 ctcgagcaga cgccgcccga gctgtcatcg gacatcatcg acaagggcat ggtgatgagc
     1441 ggcggcggct cgctgctgcg caacatcgac aagctgctca cgcaggtcac cggcgtcccc
     1501 tgccacgtcg ccgagaacag cctcaactgc gtggcgctcg ggaccgggct cgcgctcgaa
     1561 cacttcgact tcttcaagaa gtccctggtc cagcagatct gatcctcgtg tcccgggcgg
     1621 ctggttggcg tgggcatcct ccgcgattcc agtcaccgtc ggcggcttcg cgcgcctcgg
     1681 cgactgagat cgcgtccggt agcccccgcc aacccgcctc cgaatcgccg gtcgagttgc
     1741 tgccggagcg agggcctacc ctcatctgga ggtctgtagg gcttcggcac atcggtgggg
     1801 ccgttagccg accgccgttc gcgggccgca cggccacgga agaccctgct acggcctttg
     1861 ggatcggtgg cgccgatgac cggcgctctg gcgcgtatct ggacgccggc aggccggcgc
     1921 gacgcgccga ttaccgcctg gctaacggtg gggccgatca gggcatgcct ggcgggtcgc
     1981 ggcgggccgg agccgcggtg tgagggcctt cgtcgacctg cattgccaca cgtcggtgag
     2041 ctttgacagc ctggcttcgc ctgaggccgt cgtgcgggcg gcggcgaaac gagggctcac
     2101 gcatctcgcc atcaccgacc atgaccggat cgagggggcg ctgcgggcgc gcgatgcggc
     2161 tcccgagggg ctcacggtca tcattggcga ggaggtcaag accgcggatg gcgacctgat
     2221 cgccgtgttc ctcgagcggg cggtcgcgcc cgggcggccc gtccgcgaga cgatcgccga
     2281 ggtccgggag cagggcgggc tcgtggggat cccgcatccg ttcgaccggt tccgtggctc
     2341 aatgctcaag gatccccgcc tcgaggccat ggcgcccctc gtggactggg tcgaggcgca
     2401 caacgcccgc gtcgtcggcg gatcgggcaa cgagcgggcc gcggcattcg cccacgagat
     2461 gggcctgccc gggatcgcgg tgtccgacgc gcacaacgtt gtcgaggtcg gcgtcgccta
     2521 catcgcggtc gacggggacc cgtccacacc cgaaggactc atggcagccc tgaccaacgt
     2581 cgagcttgtc cccggccggg cttcctatat cgtccggact ctcacacccc tcgcaaagct
     2641 cgtcaatcgc gcccggggga accggcgggt cccgatcagc ccggccggcg atgcgggtgc
     2701 cagcacatga gtgaaccgcc cgtgaccgac gcagacgcca cgtcccccgc gaacccgctg
     2761 gcccgccaag gcggctcgac gcgggtcccg cgcaccggct cggcctcgga cggcaacggc
     2821 ggcgacggcg gccagatctc gctcggcgag cgactccgcc agccgcggac catcatctcg
     2881 ctcggcgtcc cgctcgtcct gctggtcctg atcttccggg tcgcgctcaa cgtcgacttc
     2941 gcggagctcg cgaacagcgt ggccagcgcg aacaagctgc tcctggttgc cgccctcctc
     3001 gtgttctacg caggcttccc gatccgcggc atgcgttggg cgctcctgct gaagggcgcc
     3061 ggcatgcgcg tggggaccaa ggacgcgacc gagatcctgt tcctgtcgtg gacggtgaac
     3121 tgcctggtcc cggccaagct tggcgacctg taccgcgcgt tcctgctcaa gatcaacgcg
     3181 gacgtgtcgg gctcccgcac cctgggcacg atcttcatcg agcgggtctt cgacctgttc
     3241 gccatcgcga tcatgggcct cctcgccggc ttctggagct tccggaccgg gttcccgccc
     3301 gagatccagt tcgttgccgt gctcggcatc ggggtcatcg tcgtgctcgc cgggttcctg
     3361 ttcgccgtcc ggaacttcgg ccgcgcgatc ctgggcaagc ttccgctccc ggaccgggcg
     3421 ctggagttct acgaccggtt cgaggagggc ttgttctcga tcaaccggcg gtcgctcgga
     3481 ccgctggcga tcctgaccgt cctcatctgg gtgaccgagg ggatgcgcct gtggctcgtc
     3541 atccaggcgc ttggcctccc gggcgtctcg atggggatct cgggcgcgtt cttcgtcgcc
     3601 ttcatcgggt cactgctcac cgccgtcccg ctcagcccgg ctggcctcgg aacggtggag
     3661 ctcggggtgg tcggagtgct gacgctggcc tacgggctgc ccacccagga agcgaccacc
     3721 atcgtcctgg tcgatcgcct gatcagtgtg ttctcgatca tcctgatcgg ctcgatcgtg
     3781 tatctggtgt cgccgatccg gcgcggcgcg ggtctctcgc aacccgtcga ggcgagcgca
     3841 ccagcgggct gaacggggcc tgcccctggg ggtaccggcg gagggcaacc cgatcgggtc
     3901 acgcctgcac cgcacgcgac ctcaccagac ctcccgggcc cgcacgcggc accgtgcaag
     3961 cgccgagtac gaacgggtga tgggacgggg agac
//
LOCUS       DBNHXO010000297         3367 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4250210, whole genome shotgun sequence.
ACCESSION   DBNHXO010000297 DBNHXO010000000
VERSION     DBNHXO010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4250210"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="AC162A_06270"
     CDS             complement(<1..542)
                     /locus_tag="AC162A_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010481.1"
                     /translation="MIETAAFGPATDTPRGRPDALPARPTWREVAILVAIVALAALLR
                     LPGLDQRGAWDADQGTDMLVLQGLVDRGEVPLLGPRTSIGTFHHGAVYYYLLAPAAYL
                     SGADPVAVTGEIALFGIGAVIAVWWLARLVAGPVAGLAAGLLMAVSPAGIEASTFIWN
                     PNLIPLASAVAFAAALHAIRS"
     gene            complement(539..1519)
                     /locus_tag="AC162A_06275"
     CDS             complement(539..1519)
                     /locus_tag="AC162A_06275"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HIR5010482.1"
                     /translation="MLPNGRRLGAHLPLGHGMLRAADRAAEIRASAIQVFTDNPTSWR
                     RRSALPRDLPAFRDRLAADGIAPLAIHAPYLVNLAGPDPDVVDRSVAVLVNELRVAAA
                     WGAQLVNVHLGSHRGSGVEAGVAGVASGLRRVFDQMDGEADDVTLVVENGAGGGSGIG
                     VTVDELARVEVAVADAGVGRERFGYCLDIAHLWGAGYAMDDPAGVDRLIGEFDAAIGL
                     ARVRMVHLNDSRSERGSRTDQHEHLGAGRIGSAGLRRVLTHPGLEHVVYLLETPGMDD
                     GYDLVNMDRARDLVEGRALAELPPDAFDTPSTRGRSGPPEHDDESLEPVG"
     gene            1570..2190
                     /locus_tag="AC162A_06280"
     CDS             1570..2190
                     /locus_tag="AC162A_06280"
                     /inference="COORDINATES: protein motif:HMM:NF021386.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HIR5010483.1"
                     /translation="MDPDLLAGLRALVDTNRLRIVARVGGRPASIEDLAMELRQPVPA
                     IARQVETLIAAGLIERTGDRYDNLRARWDRVGELARALSELETDAAGAASSGPNGPWP
                     HDGASYAETLERMGASDEDARILRSYLVDGRLETIPAQHRKRQVVLRFLLERVFVEDR
                     EYPEKEVNQRLALFNPDVAALRRYLVDDRYVTRDAGMYRRASDAER"
     gene            complement(2187..2786)
                     /gene="hpf"
                     /locus_tag="AC162A_06285"
     CDS             complement(2187..2786)
                     /gene="hpf"
                     /locus_tag="AC162A_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR5010484.1"
                     /translation="MRTIVRGKNLEVPDRVRQYAERKFARLDRLFDDNTDAVVELSNE
                     AHRSASDAHIADVTLVVDGRTLRSRATGGTYQEALDTVVDKVERQAIDHKEKPRVRAR
                     PEEEKALLRKIADGTAEPGGERQIVKTKRFAIEPMFEEDAVAAMEDLDHDFYVFVNAE
                     TERICVLYRRRDGNFGLIEPVMGGEYTPERRSGSKAGRA"
     gene            complement(2926..>3367)
                     /locus_tag="AC162A_06290"
     CDS             complement(2926..>3367)
                     /locus_tag="AC162A_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010485.1"
                     /translation="RQAPLAEEAAMSPEILRVVLAIVLVAHGIGHVLFLAPALRIAGW
                     ADQSGGSWLLSSTIGDGGAHAAGAIVWAAATVLFVAAGTGLLLAMDWRRPFAVVGAVV
                     SLIGILVFWDGILTASAVPALVVDVAVIVGLLIAHWPSVDAIGA"
BASE COUNT          485 a         1232 c         1225 g          425 t
ORIGIN      
        1 gaccggatcg cgtggagcgc ggcggcgaac gcgaccgccg acgccagggg aatcaggttc
       61 gggttccaga tgaacgtcga ggcctcgatt cccgcggggg acacggccat cagcagtccg
      121 gccgcgagcc cggccaccgg acccgcgacg agacgggcga gccaccacac ggcgatcacg
      181 gcaccgatcc cgaacagcgc gatctcgccg gtcaccgcga ccgggtcggc gcccgagagg
      241 tacgccgccg gcgcgagcag gtagtagtag accgcgccgt ggtggaacgt cccgatcgac
      301 gtgcgcggcc cgaggagtgg cacctcaccc cgatcgacga ggccctggag cacgagcatg
      361 tccgtgccct gatcggcgtc ccacgcaccc cgctggtcga ggccgggcag ccgaaggagc
      421 gcggcgagcg cgacgatggc gacgaggatg gcgacctcgc gccatgttgg ccgcgccggg
      481 agcgcgtccg gccggccgcg gggggtgtcg gtcgctggtc cgaaggcggc ggtctcgatc
      541 atccgaccgg ctccagcgac tcgtcgtcgt gctcgggggg cccgctccgg cccctcgtgc
      601 tcggcgtgtc gaaggcgtcc ggcggcagct ccgccaaggc tcgcccctcc accaggtcgc
      661 gggcccgatc catgttgacg aggtcgtagc cgtcgtccat ccccggcgtc tcgagcaggt
      721 agacgacgtg ctcgagcccg gggtgggtga gcacccgccg cagcccggcc gagccgatcc
      781 ggcccgcacc gaggtgttcg tgctggtccg tccgggaacc gcgctcagag cgcgagtcgt
      841 tcaggtggac catccgcacc cgcgccagtc cgatggccgc gtcgaactcg ccgatgagcc
      901 ggtcgacgcc cgccgggtcg tccatcgcat acccggcccc ccacagatgg gcgatgtcga
      961 ggcagtagcc gaaccgctcg cgcccgaccc ccgcatccgc gaccgccacc tcgacccgcg
     1021 cgagctcgtc gaccgtgacg ccgatccccg acccgccgcc ggcgccgttc tcgaccacca
     1081 gcgtcacgtc atcggcttcc ccgtccatct ggtcgaacac gcgccgcagc cccgaggcca
     1141 cccccgcgac gcccgcctcg acaccggagc cgcggtggga cccgaggtgg acgttgacca
     1201 gctgcgcacc ccaggcggcc gcgacgcgca gctcgttgac gaggacggcg accgaacgat
     1261 cgacaacgtc ggggtccggg ccggcgaggt tgacgaggta gggggcgtgg atcgcgagcg
     1321 gggcgatccc gtcggccgcg aggcggtcgc ggaaggccgg caggtcgcgc gggagcgccg
     1381 agcggcgacg ccacgaggtc gggttgtcgg tgaacacctg gatcgccgac gcgcggatct
     1441 cggcggcccg gtccgctgcc cggagcatgc cgtggccgag gggcaggtgg gcgccgaggc
     1501 gccggccgtt cgggagcatc ggcgcgaagc gtaccggagc ggcggggccc gccacctaca
     1561 ctctgcggcg tggaccctga cctcctggcc ggcctgcggg cactcgtgga cacgaaccgg
     1621 ctgcggatcg tcgcgcgcgt gggcggccgg ccggcctcga tcgaggacct ggcgatggag
     1681 ttgcggcagc cggtcccggc catcgcccgc caggtggaga ccctcatcgc ggcgggcctg
     1741 atcgagcgca cgggcgatcg atacgacaac ctccgcgccc ggtgggaccg ggtgggggag
     1801 ctggcgcgtg ccctgtcgga gctcgagacg gacgcggctg gcgccgcgtc aagcggaccc
     1861 aacgggccgt ggccgcacga cggcgcgtcg tatgcggaga cgctcgagcg catgggggcg
     1921 agcgacgagg acgcccggat cctgcgttcg tacctggtcg acgggcgcct ggagacgatc
     1981 cccgcgcagc accggaagcg ccaggtcgtg ctccggttcc tgctcgagcg cgtgttcgtc
     2041 gaggatcgcg agtaccccga aaaggaggtc aaccagcggc tcgcgctgtt caacccggac
     2101 gtcgccgccc tgcgccgcta cctggtcgac gaccgctacg tgacccgcga tgccgggatg
     2161 taccgccgcg cctcggacgc cgagcgctag gcgcgaccgg ccttcgagcc ggaccgccgc
     2221 tccggcgtgt actcgccgcc catgaccggc tcgatcagcc cgaagttgcc gtcccggcgc
     2281 cggtagagga cgcagatccg ctcggtctcg gcgttcacga acacgtagaa gtcgtggtcg
     2341 aggtcctcca tagccgcgac ggcgtcctcc tcgaacatgg gctcgatcgc gaagcgcttg
     2401 gtcttgacga tctggcgctc gccgccaggt tccgcggtcc cgtcggcgat cttccggagc
     2461 agcgccttct cctcctcggg ccgggcgcgg acccgcggct tctccttgtg gtcgatcgcc
     2521 tggcgctcga ccttgtcgac caccgtgtcc agcgcttcct ggtacgtgcc gccggtggcc
     2581 cggctgcgga gggttcgccc atcgacgacg agcgtgacgt cggcgatgtg ggcgtcggag
     2641 gcggatcgat gcgcctcatt cgagagctcg acgaccgcgt ccgtgttgtc atcgaacagc
     2701 cggtcaagcc gggcgaactt gcgttcggcg tactgccgca cacggtcggg gacctcgagg
     2761 ttcttgcctc tgacgatggt cctcacggct cacctcccgg cgcggacacg agcgactggc
     2821 ggcggggcga gggcccggcg gaccgggccc gcccggagtt cgagtatagc cgcgaccccc
     2881 tcccggaccc cccgggacgg cgcccgccgg gtcgcgtgcg cgcggtcacg cgccgatggc
     2941 gtccactgac ggccagtggg cgatcagcag ccccacgatc acggccacgt cgacgacgag
     3001 cgcgggtacg gcgctggccg tgaggatccc gtcccagaac accaggatcc cgatcagcga
     3061 caccaccgcg cccacgacgg cgaacgggcg ccgccaatcc atggccagca ggaggcccgt
     3121 cccggcggcg acgaacagca cggtcgccgc ggcccacacg atcgcgcccg cggcgtgtgc
     3181 gccaccgtcg ccgatggtcg acgacaggag ccacgagccg cccgactggt cggcccagcc
     3241 ggcgatccga agcgccggcg cgaggaacag gacgtgaccg ataccgtggg cgacgagaac
     3301 gatggcgagg acgacccgca ggatctcggg cgacatggcg gcttcctccg caaggggtgc
     3361 ttgtctc
//
LOCUS       DBNHXO010000298         1892 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4647656, whole genome shotgun sequence.
ACCESSION   DBNHXO010000298 DBNHXO010000000
VERSION     DBNHXO010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4647656"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..543
                     /locus_tag="AC162A_06295"
     CDS             118..543
                     /locus_tag="AC162A_06295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HIR5010486.1"
                     /translation="MVFDSGTEADEHALERLRTEVIGWLTTVTPDGQPQSFPIWFLWV
                     DGEALVYSDRRAKRNGNIRANPRVVLHLNDDGTGGDVVIVEGEARIDRSTAPVPGNPA
                     YLAKYGDLIREQLNSPEEFATIYNVPVWIRPTRGRAFGA"
     gene            540..1724
                     /locus_tag="AC162A_06300"
     CDS             540..1724
                     /locus_tag="AC162A_06300"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012988713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR5010487.1"
                     /translation="MTRRRPTAVVAAGAATAAGLALASPVLDRLVGRVERRLMRITQP
                     PPHPSQRALALHATLRAVDLHADSLLFGRDLLVRGSRGHLDVPRMIEGGVALQVFSAA
                     VKSPRHLNIERNDDRSDDVTRLALASRWPPRTWNSLTERALFLAGRAQRLAERSGGAF
                     RLIGTREDLAAYLGDREANRRLTAGVLAIEGAHALGPDLSTLDALVEAGFRMISPAHF
                     FDTAFGGSAHGIGKGGLTPLGRDLLREMESRGVVMDVAHASVATIDDVLGAATRPVVA
                     SHTGVRATADNGRNLSDDQLRGIAATGGLVGIGFWDTATGGRDAASIARAIVHAVGVI
                     GPEHVGLGSDWDGAVPVPFDAAGLPALTDALLDAGLEEPAIRAVMGENALRLFAETLP
                     GE"
BASE COUNT          239 a          697 c          706 g          250 t
ORIGIN      
        1 gcgtcgccct cgagcgcgac ccgcgctcgc tcgcgttcct cgacgaggtg cggtccaggc
       61 gtcggacggc gccggcaccc gcctgaccca caagccagca gcaccaggag gagagggatg
      121 gtcttcgact cgggcaccga ggccgacgag cacgcgctgg agcgcctgcg gaccgaggtg
      181 atcgggtggc tgacgaccgt cacgcccgac ggccagccgc agtcgttccc gatctggttc
      241 ctgtgggtcg acggtgaggc cctcgtctac agcgaccgcc gggcgaagcg caacggcaac
      301 atccgggcca acccgcgggt cgtgctgcac ctcaacgacg acggcactgg aggcgacgtc
      361 gtgatcgtgg agggcgaggc acgcatcgac aggtcgaccg cgccggttcc cgggaacccg
      421 gcgtacctgg cgaagtacgg ggacctgatc cgcgagcagc tgaactcgcc ggaggagttt
      481 gcgacgatct acaacgtccc ggtctggatc cgcccgacgc gcggcagggc cttcggggcg
      541 tgacccgccg ccggccgaca gccgtcgtgg cggccggtgc ggcgacggcc gcggggctcg
      601 ccctcgcaag ccccgtcctc gaccgcctgg tcgggcgggt cgaacggcgg ctgatgcgga
      661 tcacccagcc gccaccgcac ccctcccagc gcgcgctggc gctgcacgcg accctgcgcg
      721 cggtcgacct ccatgccgac tccctgctgt tcgggcgcga cctcctcgtc cgcggttcgc
      781 ggggccatct cgacgtgccg cggatgatcg agggcggggt cgccctccag gtgttctcgg
      841 cggcggtcaa gtcgccgcgc cacctgaaca tcgagcgcaa cgacgaccgc agcgacgacg
      901 tgacgaggct cgccctggcc tcgcgctggc cgccgcgcac gtggaacagc ctgaccgagc
      961 gggcgctgtt cctcgccggg cgcgcccagc ggctcgccga gcggtccggc ggggcgttcc
     1021 ggctgatcgg cacgcgcgag gacctcgccg cgtacctcgg ggaccgggag gcgaaccggc
     1081 ggctgaccgc cggcgtgctc gcgatcgagg gcgcccacgc gctgggtccg gacctctcga
     1141 cgctcgacgc gctcgtcgag gccggcttcc gcatgatctc gcccgcgcac ttcttcgaca
     1201 ccgcgttcgg cggctccgcg cacgggatcg ggaagggcgg cctcacgccg ctggggcggg
     1261 acctgctgcg cgagatggag tcgcgcggcg tggtcatgga cgtggcccac gcgtccgtcg
     1321 ccacgatcga cgacgtcctg ggcgcggcca cgcggccggt cgtcgcgtcg cataccgggg
     1381 tccgcgcgac cgccgacaac gggcggaacc tgtccgacga tcagctgcgg gggatcgcgg
     1441 cgaccggcgg gctggtgggg atcgggttct gggacaccgc gacgggcggc cgcgacgcgg
     1501 cgtcgatcgc gcgcgccatc gtccatgccg tgggcgtcat cggtcccgag cacgtcggcc
     1561 tcggctccga ctgggacggc gcggtgcccg tgccgttcga cgccgccggc ctgccggcgc
     1621 tcaccgacgc cctgctcgat gccggcctcg aggagccggc gatccgggcc gtgatgggcg
     1681 agaacgccct gcgcctgttc gccgagacgc tgcccggtga gtgacggacg ctagatcagg
     1741 ggggcgatca gcgcggcggc tctcgcagcg ccgtcgcgct ccacgggctt cgaggtgacc
     1801 ggccggtcga tctcctgcgc gatcgcctcg gcgatggcgt ccgggtccga ggtgtcgtag
     1861 tccatgtacc ggcccgcgcc gtagcgctcc ag
//
LOCUS       DBNHXO010000299         2256 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1869737, whole genome shotgun sequence.
ACCESSION   DBNHXO010000299 DBNHXO010000000
VERSION     DBNHXO010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1869737"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="AC162A_06305"
     CDS             <1..218
                     /locus_tag="AC162A_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010488.1"
                     /translation="AARLGVYLHGLAGEAVRDRIGDAGLLAGDLPEALPIGRKRLARM
                     AERERTGRRLGFGVRTADAATTDGAPA"
     gene            284..1543
                     /gene="alr"
                     /locus_tag="AC162A_06310"
     CDS             284..1543
                     /gene="alr"
                     /locus_tag="AC162A_06310"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR5010489.1"
                     /translation="MPRTAWLELDLDALRDNLATIRRVVGPGVRVEPVVKADAYGHGA
                     VPIARALEAASADGVSVATLDEAIELRDGGVGLPVLVLYPVPADQVAIAARASIAVSV
                     GSGALTDRILAAAGSASAAGAPDLEVHVEVETGLGRGGALPGEATATVAAIVAAPGVR
                     LGGVWTHLVAADDLSGTRAQDARFGSAVGALVGTVELGPDGVRRHLAGSGGILAADAA
                     SWDAVRPGLSTYGLVPDAVTPPSATAAQAAALRPVMALFARPVRVVDLPAGHGVSYGP
                     TFVTARPSRIATLPVGYGDGWRRILTDRAFALVRGTRVPLVGRVAMDAVMADVTDVHG
                     EPVHDQDEFVLLGLQGDDRITAHDLAAACGTISYEIVTSMSRRLPRVYHAARSPVEVR
                     TLAGGRSEWHASSSGTATSAISRSTRS"
     gene            1483..2043
                     /locus_tag="AC162A_06315"
     CDS             1483..2043
                     /locus_tag="AC162A_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0072570 - ADP-D-ribose binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HIR5010490.1"
                     /translation="MARIELWNGDICDLEVDAIVSPASTSLWMSTGVAGELKRIGGDE
                     IEFAAVRQGPVKLGDAIVTPAGKLAAKVVIHAVSLERDRRTSDAAIDRAARNAMARVR
                     ELGLTSVAFPALGTGIGGFPMDEAAYVAVSAVRDELATPSTIEHVVFALRGAAAYEAY
                     AHALSAGEPAPARPLIAKAPQASATE"
     gene            2040..>2256
                     /locus_tag="AC162A_06320"
     CDS             2040..>2256
                     /locus_tag="AC162A_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010491.1"
                     /translation="MSFQMEPVDERREALVEAVAREIRLRGLTGPAVHFLEASRPYRP
                     LGAPAMLFFDPVLGELFGGEIPSASELL"
BASE COUNT          271 a          801 c          874 g          310 t
ORIGIN      
        1 acgcggcgcg cctcggggtc tacctccatg gcctggccgg cgaggcggtc cgcgaccgga
       61 tcggcgacgc ggggctgctg gccggcgacc tgcccgaagc cctcccgatc gggcgcaagc
      121 ggctcgcacg gatggcggag cgcgagcgga cgggacggcg gctcggattc ggcgttcgaa
      181 cggccgacgc tgccacgacg gacggggcgc ccgcctgagc gagaaggccg ctccggagcg
      241 cgagccgatc gaggcgcgtc tcgcggcggc ggggctcccg gccctgccgc gcacggcctg
      301 gctcgagctc gatctcgacg cgcttcgcga caacctcgcc acgatccgcc gcgtcgtcgg
      361 cccgggagtg cgggtcgagc ccgtggtcaa ggccgacgcc tacggccacg gtgcggtccc
      421 gatcgccagg gcgctcgagg cggcgtccgc cgacggcgtc tcggtcgcga ccctcgacga
      481 ggcgatcgag ttgcgcgacg gtggcgtggg cctgccggtg ctggtgctgt accccgtccc
      541 ggcagatcag gttgcgatcg ccgcacgggc ctcgatcgcg gtgagcgtcg gatcaggggc
      601 gctgaccgac cggatcctgg ccgcggcagg atcagcctcg gcagcgggcg ctcccgacct
      661 cgaggtgcac gtcgaggtgg aaacgggcct gggacgcggg ggcgcgctgc cgggcgaggc
      721 gacggcgacg gtggcggcga tcgtggcggc gcccggcgtc cggctgggcg gcgtgtggac
      781 gcatctcgtg gccgccgacg acctgtcagg cacgcgcgcc caggacgcgc ggttcgggtc
      841 ggcggtcggc gcgctcgtgg gcacggtcga gctgggcccg gacggcgtgc ggcgccatct
      901 cgccggtagt ggcgggatcc tggccgcgga cgccgcgagc tgggacgccg tgcggccggg
      961 cctgtcgacc tacggcctcg tccccgacgc cgtaacgccg ccctcggcga ccgcggccca
     1021 agcggcggcg cttcgcccgg tcatggccct gttcgctcgt cccgtccgcg tcgtcgacct
     1081 gccggcgggc cacggcgtca gctacgggcc gacgttcgtc acggctcgac cgtcgcggat
     1141 cgccacgttg ccggtcggct atggtgacgg ctggcgacgg attcttacgg atcgggcctt
     1201 cgcgctcgtc cgcgggacgc gcgtccccct cgtcggcagg gtcgcgatgg acgcggtcat
     1261 ggccgacgtc acggacgttc acggcgagcc cgttcacgat caggacgagt tcgtgctgct
     1321 ggggctccag ggggatgacc ggatcaccgc ccacgacctc gcggcggcgt gcggaacgat
     1381 cagctacgag atcgtcacgt cgatgtcccg ccgcctgccc cgggtgtacc atgccgcgcg
     1441 ttcacctgtt gaggtccgga cgctcgcggg cgggaggtcc gagtggcacg catcgagctc
     1501 tggaacggcg acatctgcga tctcgaggtc gacgcgatcg taagtcccgc ctccacgtcc
     1561 ctgtggatgt cgacgggcgt cgcgggcgag ctcaagcgga tcgggggcga cgagatcgag
     1621 ttcgccgccg ttcgccaggg cccggtgaag ctcggggacg ccatcgtgac gcccgcgggc
     1681 aagctcgcag ccaaggtggt gatccacgcc gtgtcgctcg agcgcgatcg ccggacgagc
     1741 gatgccgcga tcgatcgcgc ggcgcgcaac gccatggcgc gggtgcgcga gcttggcctg
     1801 acgtccgtcg cgttccccgc gctcgggacc gggatcggcg gcttcccgat ggacgaagcc
     1861 gcctacgtcg ccgtcagcgc ggtccgcgac gagctggcca ccccatcgac catcgagcac
     1921 gtggtcttcg cgctgcgcgg agccgccgcg tacgaggcgt atgcccacgc cctgtcggcc
     1981 ggcgagccgg cgccggcgag gccgctgatc gccaaggcac cccaggcgag cgcgaccgag
     2041 tgagcttcca gatggagccg gtcgacgagc gccgcgaggc gctggtcgaa gcggtcgcgc
     2101 gagagatccg cctgcggggc ctgacgggcc ccgccgtgca cttcctggag gcgagccgcc
     2161 cgtaccggcc gctcggtgcg cccgcgatgc tgttcttcga tccggtgctg ggcgagctgt
     2221 tcgggggcga gatcccgtcg gcgtcggagc tgctgc
//
LOCUS       DBNHXO010000300         2336 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2465622, whole genome shotgun sequence.
ACCESSION   DBNHXO010000300 DBNHXO010000000
VERSION     DBNHXO010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2465622"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="AC162A_06325"
     CDS             <1..390
                     /locus_tag="AC162A_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5010492.1"
                     /translation="TPGVAQGGIQGGTYAAKTIIARLRGEPTKPYRYSNHGDVAVIGR
                     LAGVTDIPWMGPFGRQGGFTAWLLWLGIHIVYLIGFANRIVVTTRWAFSFLTRGRSTR
                     LITGQPLVPHIEEPLAVDTEDREAERV"
     gene            complement(387..1796)
                     /gene="pdxR"
                     /locus_tag="AC162A_06330"
     CDS             complement(387..1796)
                     /gene="pdxR"
                     /locus_tag="AC162A_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013734681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070279 - vitamin B6 binding [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:2000142 - regulation of DNA-templated
                     transcription initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MocR-like pyridoxine biosynthesis transcription
                     factor PdxR"
                     /protein_id="HIR5010493.1"
                     /translation="MARTRTSSNLELLVILDRDHPHALHHQLEQALREAIRDGRLPAT
                     TALPSTRGLAAQLGVSRGIVVEAYAQLTAEGYLASTPGGATRVARNATVTEVYRPVLQ
                     PPTIEFDFRPGRPDVAEFPRAAWLRSLRRVLTDAPSERLTYLNGHGIPELRVALATYL
                     NRARGACADPADVVICAGFAQGLALTARAMRAAGARRIAVEDPSDPEYRTTLARAGLD
                     SVGVPVDERGVRVDLLDGLDVDGIVVTAAHQYPTGAVLPPERRAALVDWAVRRGALII
                     EDDYDAEFRYDREPIGAIQGLNRDNVVYAGTASKMLAPGLRLGWLVSPPHLTDRLIEA
                     KQAADMGSAALDQLAFADILERGELDHHLRRMRAIYRGRRDALLAALARHLPDVRPVG
                     AAAGLHVLAWLPGDVDEAGVVAEALASGIAVGSLATRRIAPGPDGLIFGYGVIAEPAI
                     EAGVARLAGIVAANRGKAA"
     gene            1876..2022
                     /locus_tag="AC162A_06335"
     CDS             1876..2022
                     /locus_tag="AC162A_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010494.1"
                     /translation="MDSLFAILSFAALFAIFGTIAAIVGEDTRDGFGEHPSVRYLTLN
                     PRHR"
     gene            2112..>2336
                     /locus_tag="AC162A_06340"
     CDS             2112..>2336
                     /locus_tag="AC162A_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010495.1"
                     /translation="MDEAIPRSCDIAVLVPRRGADAILARLDDDATSDAPWRLPSVNL
                     VMDDDQPMSHVFEAAAAAVGLPLIPLRLVVS"
BASE COUNT          324 a          812 c          880 g          320 t
ORIGIN      
        1 acgccgggcg tcgcccaggg tggcatccag ggcgggacgt acgccgcgaa gaccatcatc
       61 gcccgactcc gcggcgagcc gacgaagccg taccggtaca gcaaccacgg cgacgtggcg
      121 gtgatcgggc ggctcgcggg ggtcaccgac atcccgtgga tgggcccgtt cgggcggcag
      181 ggcgggttca ccgcctggct gctgtggctg gggatccaca tcgtgtacct gatcggcttc
      241 gcgaaccgga tcgtcgtcac cacccgctgg gcgttctcgt tcctgacccg cgggcgctcg
      301 acgcggctga ttaccggaca gccgctggtg ccccacatcg aggagccgct cgcggtcgac
      361 acggaggacc gagaggccga gcgggtctag gccgccttgc cccggttcgc cgcgacgatc
      421 ccggcgaggc gcgcaacccc ggcctcgatc gccggctccg cgatcacccc gtagccgaag
      481 atcaggccgt cagggccggg cgcgatgcgg cgcgtcgcca ggctgcctac tgcgatgccg
      541 gaggcaaggg cctcggcaac cacacctgcc tcgtcgacgt cgccgggcag ccaggcgagg
      601 acgtgcaggc ctgccgccgc cccaacggga cggacgtcgg gcaggtgccg ggcaagggcc
      661 gcaaggagcg cgtcccgccg gcctcgatag atcgcgcgca tccggcgcag gtgatggtcc
      721 agctcgccac gctcgaggat gtcggcgaac gccagctggt cgagggccgc ggagcccatg
      781 tcggcggcct gcttcgcctc gatcagccgg tccgtgaggt gaggcgggga gacgagccaa
      841 ccgaggcgca ggcccggagc cagcatcttg ctcgcggttc ccgcgtagac cacgttgtcg
      901 cggttgagtc cctggatggc gccgatcggc tcgcggtcgt agcggaactc ggcgtcgtag
      961 tcgtcctcga tgatcagcgc gccgcggcgg acggcccagt cgacgagggc ggcgcgacgc
     1021 tcgggaggga ggacggcgcc ggtcgggtac tggtgggcgg cggtgacgac gatgccgtcg
     1081 acgtcgagac cgtcgagcag gtccacccgt accccgcgct cgtcgaccgg cacgccgacc
     1141 gagtccagcc ccgctcgtgc aagcgtggtg cggtactcgg ggtccgacgg gtcctcgacg
     1201 gcgatccggc gcgcgccggc ggcacgcatc gcgcgcgcgg tcagggcgag cccctgggcg
     1261 aaccccgcgc agatcacgac gtcggccgga tcggcgcagg cgccacgggc gcggttcagg
     1321 tacgtggcga gcgcgacgcg cagctccggg ataccgtggc cgttgaggta ggtgagccgc
     1381 tcgctcgggg cgtcggtcag gacgcgacgc aggctgcgca gccaggcggc ccgcgggaac
     1441 tcggcgacgt ccgggcggcc ggggcggaag tcgaactcga tggtcggggg ctggaggacc
     1501 gggcggtaga cctcggtgac cgtcgcgttg cgggcgacgc gcgtcgcgcc gcccggcgtg
     1561 ctggcgaggt agccctcggc ggtcagctgc gcgtaggcct cgacgacgat gccgcgcgac
     1621 acgcccagct gggcggcgag cccgcgggtc gaggggaggg cggtcgtggc tggaaggcgg
     1681 ccgtcgcgga tggcctcgcg gagcgcctgc tcgagctggt ggtggagcgc gtgcgggtgg
     1741 tcgcgatcca ggatcacgag cagctcgagg ttggaactgg tcctcgttct tgccattgaa
     1801 ccggacctta tcagaggtcc ggtcctgcgt cacgatgcat ccatcagctc gaacccagca
     1861 acctggaggt ccgacgtgga cagcctcttc gccatcctct cgttcgccgc cctgttcgcc
     1921 atcttcggaa ccatcgccgc gatcgtcggc gaggacacgc gcgacggctt cggcgagcat
     1981 ccctccgtgc ggtacctgac cctgaatccg cgacaccgct agccggcgtc cgacctcgtg
     2041 gcgggtgacg cgctgacgct cgatcgcgag cgagcccgaa accgtcctgc tggccgcgct
     2101 aggctgccgg cgtggacgaa gccattcccc gcagctgcga catcgcggtc ctggtgccgc
     2161 gccgcggggc ggacgcgatc ctcgcgcgcc tcgatgacga cgcgaccagc gacgcgccat
     2221 ggaggctgcc gtcggtcaat ctcgtgatgg acgacgacca gccgatgagc cacgtgttcg
     2281 aggccgccgc agccgctgtc ggcctgccgc tgatcccgct ccggctcgtg gtgagc
//
LOCUS       DBNHXO010000301         6439 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2068053, whole genome shotgun sequence.
ACCESSION   DBNHXO010000301 DBNHXO010000000
VERSION     DBNHXO010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6439
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2068053"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="AC162A_06345"
     CDS             complement(<1..411)
                     /locus_tag="AC162A_06345"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR5010496.1"
                     /translation="MTVPLILDVDTGIDDALALLYACGSPDADLVAVTCLSGNAALED
                     TERTTRAVLELAGRADVEVAAGRPTPLLRALEITPETHGPRGLGYAELPEPGTPLSAR
                     FGPDLIVEEARRRPGEITLVTLGPLTNLAIALIVE"
     gene            complement(452..991)
                     /locus_tag="AC162A_06350"
     CDS             complement(452..991)
                     /locus_tag="AC162A_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010497.1"
                     /translation="MTVEVLIVVLVRVAGSLPVLRWALAGGILAILIDLSDLLLFNVL
                     DLGGVSDYQRLDKVLDLVYMTAFLIVALRWTGPDRWVSVALFAWRMIGFAAFEATGER
                     ALLLVFPNVFEPWFLLVALLHHRWNPVPWTPLTLAAALAGLLAVKELQEWALHYARMF
                     DDITALEAIDQAWRWLTGR"
     gene            complement(1257..2087)
                     /locus_tag="AC162A_06355"
     CDS             complement(1257..2087)
                     /locus_tag="AC162A_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR5010498.1"
                     /translation="MSPTARPPKPKPKPKRRKLDPVKVWANTLKRYRPGLTRYVLDEL
                     TIVYGEQVWESRLDPTSELILTILTQNTADVNAEKAYEALRLAYASDRDPEVHKPGIG
                     WGGEGLSTASPPDWQKVEFAPLEELIDVIRPGGLGPQKAPRLQATLRYIREQRGDYSL
                     EFLGDMAAIEARDWLTAIDGIGKKTASIVLLFCFGTPLMPVDRHVERVSQRIGLIPPK
                     ATADQAHDLFLGLLEPDEMYAAHVLLIHHGRVICHAQNPKHELCPVRDRCRFVDRKAP
                     "
     gene            complement(2084..2470)
                     /locus_tag="AC162A_06360"
     CDS             complement(2084..2470)
                     /locus_tag="AC162A_06360"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR5010499.1"
                     /translation="MRVLLDTHAFLWSVTDDRRLSSAVREIVADPENDVLFSAASALE
                     ISLKAARGRLELPEQTDTYVTTRIAAFGFVPLAISVSHAIRAGALPPIHADPWDRLLT
                     AQAQMEGVAILTRDALIRRYDVETIW"
     gene            complement(2467..2730)
                     /locus_tag="AC162A_06365"
     CDS             complement(2467..2730)
                     /locus_tag="AC162A_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR5010500.1"
                     /translation="MPAVVNIHEAKTHFSKLVERARNGERITIAKAGVPVAMLVAIEP
                     ETRPRRPIGIDKGRVIIHDDFDDPLPEFDPDYSHPGDPLLDHG"
     gene            complement(2782..3930)
                     /locus_tag="AC162A_06370"
     CDS             complement(2782..3930)
                     /locus_tag="AC162A_06370"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HIR5010501.1"
                     /translation="MADRQLRVLIAPDSFKGSLTSVAVARALADGWHRARPRDDLHLA
                     PLADGGEGTLIAIEAAGGWQWREADATDPLGRPIRARWLRRADGRAAVVEMAAASGLS
                     LVAAQERDPARATSKGTGDVLRAVLDEGIRDIALGIGGSATTDGGAGILRSLGAEVSD
                     DLATVDLGGLDSRLAETRLRIACDVTNPLLGPTGAAAIYGPQKGASPSEVMALDMRCA
                     RFADALERAAGRRERDTPGAGAAGGVGFAMLTLAGEFAELELRPGIDLVMEEAGFDRA
                     LASSQLVITGEGRIDAQTAYGKTALGVAKRARAAGRACIAIGGGVTPEGIEALAAVGA
                     IAVPVVEVPQDIETAMAAGTPPVERCGERIARLIGVSIDLPAATPHLS"
     gene            complement(3933..5849)
                     /locus_tag="AC162A_06375"
     CDS             complement(3933..5849)
                     /locus_tag="AC162A_06375"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HIR5010502.1"
                     /translation="MNGQPLPSRVLIPVANPLTAEELVRIGASLMDPLTGELTALGIV
                     EVPEGVPLSEGATRARQARRLLQKVLDYVPEGTVIHPLVRIARHAAAGIVEAAAEQEA
                     DLMIFGWGGKSGPNRDAHAPVISPTIDEAVRESPCDIAVVKQRGARDIRRILVPVRGG
                     PHAELALRFADALAHRHDATVAVMHVVPSGVTEAVRAQAEHALAAFARQHVTARSEPI
                     LRETSNVRTAILREAEKSDLVVMGASAPPGEPGSSLFGALPEAIAQRARPTVIVVKTR
                     EPIGRQTFEQLAQRAETLAAADRAAEESRAVPARVERWFAEANFHHSEFADLARLTSL
                     KQKQGLTVSLVLPTLDEAETIGPIVRRAVREMQERYPLLDEVLVIDSASTDDTRAIAE
                     AEGARVVQHPDVLTRYGSFVGKGEALWKSLYETTGDIVVWADTDVRNWHARMVYGTLG
                     PLLVEPRLQYVKGYYQRPIVEAGVLKEGGGGRVTELVARPLVNLFFPELSGFIQPLAG
                     EYAGRRSLLEQLPFFTGYAVEIGHLIDAAERAGIEGLGQVDLERRVHRNQELEGLSRM
                     SFVILQAVMKRLEERRRVRLFAELGSTMKLPRSGRGHLALEVQELADHERPPMIRIPE
                     YLEKRRTANGESAD"
     gene            complement(5846..>6439)
                     /locus_tag="AC162A_06380"
     CDS             complement(5846..>6439)
                     /locus_tag="AC162A_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010503.1"
                     /translation="VVGTILRLAASHPDAWLAGRGSHDVPAYGFERIIDPPPLEVNAI
                     RLLSEFHTGSLTVADTWRRTIDPANLDVVMTLAAEAGKLADAATNKLGLGGEGETVKA
                     TTLQMSEALAAFHFPDDLWSRLIYDLVVATRRGEVDTGQMVAALVPIYFGRVGSFVIE
                     TRHLTTADAEERVERQAREFELLKPYLVERWHATDAA"
BASE COUNT          894 a         2274 c         2340 g          931 t
ORIGIN      
        1 ttccacgatc agcgcaatgg ccaggttggt cagcggaccc aaggtgacga gcgtgatctc
       61 tccgggacgg cgccgggcct cctcgacgat gaggtcgggc ccgaagcgcg cggagagcgg
      121 cgtgcccggc tccggcagct cggcgtagcc caggccgcgg ggtccgtgcg tctcgggcgt
      181 gatctcgagc gcccgcagca ggggcgtcgg gcgtcccgcg gcgacctcga cgtccgcacg
      241 gcccgcgagc tcgagcacgg cccgggtggt gcgctcggtg tcctcgagcg ccgcgttccc
      301 ggacaggcac gtgacggcga cgaggtccgc gtcggggctg ccgcaggcgt agagcagcgc
      361 gagcgcgtcg tcgatgccgg tgtcgacgtc aaggatcagc gggacggtca tggcccgatg
      421 ctagacgctc ggcgccgtcg gagcccgcgg ctcatcggcc ggtgagccag cgccaggcct
      481 ggtcgatcgc ctcgagcgcc gtgatgtcgt cgaacatccg ggcgtagtgg agcgcccact
      541 cctggagctc cttgaccgcg agcaggcccg cgagcgccgc cgcgagcgtg agcggcgtcc
      601 acggcaccgg gttccagcgg tggtggagga gcgccacgag caggaaccag ggttcgaaga
      661 cgttgggaaa gacgagcaga agcgcccgct caccggtcgc ctcgaacgcc gcgaaaccga
      721 tcatccgcca ggcaaacagc gcgacgctca cccagcgatc cgggccggtc cagcggagcg
      781 cgacgatgag gaaggcggtc atgtagacga ggtcgaggac cttgtcgaga cgctggtagt
      841 cggacacgcc gccgaggtcg aggacgttga atagcagcag gtccgagagg tcgatgagga
      901 tcgccaggat cccgccggcc agcgcccagc gcagcaccgg cagcgacccc gccaccctga
      961 cgagcaccac gatcaggacc tcgacggtca tcgtgcgagt gtgcacggcc gcttcgacgc
     1021 tcccgcatgc cgcgcggcgt agtcgctcca tgacttgcct gagcgtaggt ccctcgcgaa
     1081 cggtgcccgc gcgcgtcagg gagccacccg atcggccacc ttggcgtcca agtcatgcat
     1141 gtaccgactc gcgacccgca caagtgcggc gtttgacctc gtggcgcggc ccgatccaga
     1201 ccttcgctgg tgccctccgc aggggccatg aatgcagcga ctgccggtcg gcgcttctac
     1261 ggcgccttgc ggtcgacgaa gcggcagcgg tcgcggaccg ggcacagctc gtgcttgggg
     1321 ttctgcgcat ggcagatcac ccggccgtgg tggatcagga gcacgtgggc ggcgtacatc
     1381 tcgtctggct cgagcagccc gaggaacagg tcgtgcgcct ggtccgctgt ggccttgggc
     1441 gggatcagcc cgatgcgctg cgagacgcgt tcgacgtggc gatcgacggg catcagcggg
     1501 gtgccgaagc agaacaggag cacgatcgac gcggtcttct tgccgatgcc gtcgatcgcc
     1561 gtcagccagt cgcgggcctc gatcgcggcc atgtcgccca ggaactcgag cgagtagtcg
     1621 ccgcgctgct ctcggatgta gcgcagcgtc gcctggaggc gcggcgcctt ctgcggcccc
     1681 agaccgccgg gccggatgac gtcgatcagc tcctcgaggg gcgcgaattc gaccttctgc
     1741 cagtcgggcg gggacgccgt cgacaggccc tcgccgcccc agccgatgcc cggcttgtgg
     1801 acctccggat cgcggtcgct ggcgtaggcg aggcggagcg cctcgtacgc cttctcggcg
     1861 ttcacgtccg ccgtgttctg ggtgaggatc gtgaggatca gctcgctcgt cgggtcgagt
     1921 cgcgactccc agacctgctc gccatagacg atggtcagtt cgtccaggac gtagcgcgtc
     1981 agcccgggtc ggtatcgctt cagcgtgttg gcccatacct tgaccgggtc aagcttccgg
     2041 cgcttcggct tcggcttcgg tttcggcggc cgcgcggtcg ggctcaccag atcgtctcga
     2101 cgtcgtaccg gcggatcagc gcgtcccggg tgaggatcgc aacgccctcc atctgggcct
     2161 gcgccgtcag gagcctgtcc cagggatcgg cgtggatcgg cggcagcgcg cctgcccgga
     2221 tcgcgtggct cacgctgatc gcgagcggca cgaatccgaa cgccgcgatc cgcgtggtca
     2281 cgtaggtgtc cgtctgctcg ggcagctcga gccgccctcg tgcggccttg agcgagatct
     2341 cgagcgcgct cgcggcgctg aacaggacgt cgttctccgg atccgcgacg atctcccgca
     2401 cggcgctcga gaggcggcga tcgtctgtca cggaccacag gaaggcgtgg gtgtcgagca
     2461 gcaccctcat ccgtggtcga gcagcgggtc gcccgggtgc gagtagtcgg ggtcgaactc
     2521 cgggagaggg tcgtcgaagt cgtcgtggat gatcacgcgg cccttgtcga tcccgatcgg
     2581 ccgtctcggg cgcgtctcgg gctcgatggc cacgagcatt gcgacgggaa caccggcctt
     2641 cgcgatcgtg atccgctcac cgttgcgcgc gcgttcgacc agcttcgaga agtgggtctt
     2701 cgcctcgtgg atgttcacga ccgccggcat gggccgatgt tgacttagtc catggactaa
     2761 gtcaaggcgg gcaatcggaa gctatgacag gtgcggcgtc gccgcgggca ggtcgatcga
     2821 cacgccgatc aggcgcgcga tccgctcgcc gcagcgctcg accggcgggg tcccggcggc
     2881 catcgcggtc tcgatgtcct gtggcacctc gaccaccggc acggcgatcg cgccgaccgc
     2941 cgccagcgcc tcgatgcctt cgggcgtcac cccaccgccg atcgcgatgc acgcccggcc
     3001 ggcggcccgc gcccgctttg cgacgcccag cgcggtcttg ccgtacgcgg tctgggcgtc
     3061 gatgcgcccc tccccggtga tcacgagctg cgacgacgcc agggcacgat cgaacccggc
     3121 ctcctccatg acgaggtcga taccgggccg cagctccagc tccgcgaact ccccggccag
     3181 cgtcagcatc gcgaacccga cgccgccggc cgcgcccgca cccggggtgt cgcgctcccg
     3241 gcgcccggcc gcgcgctcga gcgcatccgc gaaccgcgca cagcgcatgt cgagcgccat
     3301 cacctcactc ggcgacgcgc ccttctgcgg gccgtagatc gccgccgcac ccgttggtcc
     3361 gagcaggggg ttggtcacgt cgcaggcgat ccggagacgc gtctcggcca gccgcgagtc
     3421 gaggccgccc aggtccaccg tcgcgaggtc gtcgctcacc tcggcgccca gcgagcgcag
     3481 gatccccgcg ccaccgtcgg tcgtggcgct gccgccgatc ccgagcgcga tgtcgcggat
     3541 cccctcgtcg agcaccgcgc gcagcacgtc gcctgtgccc ttgctggtcg cgcgcgcggg
     3601 atcgcgctcc tgcgccgcga cgagggacag ccccgacgcc gcggccatct ctacgaccgc
     3661 ggcccggccg tcggcgcggc gaagccagcg ggcgcggatc ggacggccca ggggatcggt
     3721 cgcgtcggcc tcgcgccact gccagccgcc ggcggcctcg atcgcgatca gcgtcccctc
     3781 cccgccgtcg gcgagcgggg cgaggtgcag gtcgtcgcgc gggcgcgcgc gatgccatcc
     3841 gtccgcgagc gcgcgcgcca cagcgacgct ggtcagcgag cccttgaacg agtcgggcgc
     3901 gatcagcacc cgcagctgcc gatcggccat cgtcagtcgg cggactcgcc gttcgccgtg
     3961 cggcgcttct cgaggtactc cgggatccgg atcatcggcg gccgctcgtg gtcggccagc
     4021 tcctggacct cgagggcgag gtggccacgg cccgagcggg gcagcttcat cgtcgatccg
     4081 agctcggcga acagccgcac ccgccgtcgc tcttcgagcc gtttcatgac cgcctgcagg
     4141 atcacgaacg acatccgcga tagcccctcg agctcctggt tgcggtgcac ccgccgctcc
     4201 aggtcgacct ggcccagtcc ctcgatcccg gcccgctccg cggcatcgat caggtggccg
     4261 atctcgacgg cgtagccggt gaagaacggg agctgctcga ggagcgacct gcggcccgcg
     4321 tactcgcccg ccagcggctg gatgaagccc gacagctcgg ggaagaacag gttcacgagc
     4381 ggcctcgcga cgagctcggt caccctgccc ccgccaccct ccttgagcac gccggcctcg
     4441 acgatcgggc gctggtagta gcccttcacg tactgcaggc gcggctcgac cagcagcggt
     4501 ccgagcgtcc cgtagaccat gcgcgcgtgc cagttgcgca cgtcggtgtc cgcccagacg
     4561 acgatgtcgc cggtggtctc gtagagcgac ttccagagcg cctcgccctt gcccacgaac
     4621 gagccgtagc gggtcagcac gtcggggtgc tggacgactc gcgcgccttc ggcctcggcg
     4681 atcgcgcggg tgtcatcggt ggacgccgag tcgatgacca ggacctcgtc gagcagcggg
     4741 tagcgctcct gcatctcgcg cacggcgcgg cggacgatgg gcccgatggt ctcggcctcg
     4801 tcgagcgtcg gcagcacgag gctgacggtg aggccctgct tctgcttgag cgatgtcagg
     4861 cgggcgaggt cggcgaactc ggagtggtgg aagttggcct cggcgaacca gcgctcgacg
     4921 cgggcgggga cggcacgcga ctcctccgcg gcgcggtcgg cggcggcgag ggtctcggcc
     4981 ctctgggcga gctgctcgaa cgtctggcgg ccgatgggct cgcgggtctt gacgacgatc
     5041 accgtaggcc gggcgcgctg ggcgatcgcc tcgggcaggg cgccgaacag cgacgagccc
     5101 ggctcgccgg gcggcgccga ggcgcccatg acgacgaggt cggacttctc ggcctcgcgc
     5161 aggatcgcgg tccggacgtt ggacgtctcg cgcaggatcg gctcggagcg ggccgtgacg
     5221 tgctggcgcg cgaacgccgc cagcgcgtgc tccgcctggg cccggacggc ctcggtgacg
     5281 cccgacggga ccacgtgcat cacggcgacc gtcgcgtcgt ggcgatgggc gagcgcatcc
     5341 gcgaagcgga gcgcgagctc cgcgtgcggc ccgccgcgga ccgggacgag gatccggcgg
     5401 atgtcgcgcg cgccacgctg cttgacgacg gcgatgtcgc agggcgactc gcgcacggcc
     5461 tcgtcgatcg tcggcgagat gaccggggca tgggcgtcgc ggttgggccc ggacttgccg
     5521 ccccaaccga agatcatgag gtcggcttcc tgctcggcgg cggcctcgac gatcccggcc
     5581 gcggcatggc gcgcgatgcg cacgagcgga tggatcacgg tcccctcggg gacgtagtcg
     5641 aggaccttct gcagcaggcg ccgggcctgg cgggcgcgcg tcgcaccctc ggacagcggc
     5701 acgccttcgg ggacctcgac gatccccagg gccgtcagct cgccggtcag cgggtccatc
     5761 agcgacgccc cgatgcggac gagctcctcg gcggtcagcg ggttggcgac cgggatcagg
     5821 acccgggacg ggagcggctg cccgttcatg cggcgtcggt ggcgtgccac cgctcgacga
     5881 ggtacggctt gagcagctcg aactcgcggg cctggcgctc cacgcgctcc tcggcgtcgg
     5941 ccgtcgtcag gtggcgcgtc tcgatgacga agctgccgac ccgcccgaag tagatcggga
     6001 ccagcgccgc gaccatctgg ccggtgtcga cctcgccgcg tcgcgtcgcg accacgaggt
     6061 cgtagatgag gcgcgaccac aggtcgtccg ggaagtggaa cgccgcgagc gcctcggaca
     6121 tctggagcgt cgttgccttg accgtctcgc cctcgccgcc cagcccgagc ttgttcgtcg
     6181 ccgcgtcggc gagcttgccg gcctcggcgg cgagcgtcat gacgacgtcg aggttcgcgg
     6241 ggtcgattgt ccggcgccag gtgtcggcga cggtcagcga cccggtgtgg aactccgaca
     6301 ggaggcggat ggcattcacc tccagcggcg gagggtcgat gatccgctcg aagccgtagg
     6361 ccgggacgtc gtggctgcca cgcccggcga gccaggcgtc gggatggctg gccgcgagac
     6421 gcaggatggt gcccacgac
//
LOCUS       DBNHXO010000302         3830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3655329, whole genome shotgun sequence.
ACCESSION   DBNHXO010000302 DBNHXO010000000
VERSION     DBNHXO010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3655329"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="AC162A_06385"
     CDS             complement(<1..465)
                     /locus_tag="AC162A_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5010504.1"
                     /translation="MAFSTFDPPVGSDWGRPAGPESAPSRQRRAILGVIAVFVVLAMA
                     IRLWPAAGESPDPVAAADPGTVTIVNGEPASIDPARHGDLGSASTISQLYETLTTIDP
                     SLVVRPALAESWTVEDGGRRVTFTLRDDLVFSDGTPLTANDVVHSWRRLFTPG"
     gene            complement(549..710)
                     /locus_tag="AC162A_06390"
     CDS             complement(549..710)
                     /locus_tag="AC162A_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010505.1"
                     /translation="MRRWVCIVALLTGFSGSGYAWSARPRLPIPVERLLDAQRPDPSL
                     PEEAWWRTT"
     gene            771..2354
                     /locus_tag="AC162A_06395"
     CDS             771..2354
                     /locus_tag="AC162A_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013565.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysS/YqeB C-terminal domain-containing protein"
                     /protein_id="HIR5010506.1"
                     /translation="MTGPALRNTLSRRVEPVRVPDDAPVRMYTCGPTVYRWAHVGNLR
                     SYLFADLIRRTLLYHGIPVLHVKNITDVGHLRDERFDRGEDRMLVAAGLESKTTAEIA
                     DAYEAGFHADEALVNILPAHVFPRATEHIDDMLELAGRLESLGHAYLSSAGNLYYAVD
                     SFPGYGALSGNTLDSLRAGHRHDVEPDKREHADFALWKAAGEGRILRWPSPWGDGFPG
                     WHLECSAMALRYLGPDFEIHTGGIDNIFPHHEDEIAQSTPITGGPPVRHWVHGEHLLA
                     AGRKMAKSTGNFERVTELRDRGIDPLAFRYLTLTVRYGRKLNYSDASIDAAAAALSSL
                     RAHLAALGPPPADGPWVAPAVLRAGFAGARPRGVADGVAGHQAGSPLAAESRAFSIAD
                     RAGSPTAPLSPPGRRLHDRFVAAIDDDLDLPAALAVVRETVRTDLPSNERRWLVLDAD
                     LVLGLDLDRAWDPAAGAATDTGADDLPAGAAPILEARSAARAARDWARADALRDQLAA
                     MGIEVVDGPDGTTWHRRPS"
     gene            complement(2347..3402)
                     /locus_tag="AC162A_06400"
     CDS             complement(2347..3402)
                     /locus_tag="AC162A_06400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5010507.1"
                     /translation="MAKDEAPAEVRALADARAAARRAKDWTTADALRAEIEAAGWKVL
                     DAASLYSLERAAAPDLVEAGVTRYGTSASVPSRLDEAPVGFASVVLVATDWPADVSRT
                     VEAFAGRSPAGTQVIVVANGPSEAQSDALAELEAAGTGSTGVGLEVVWTSTRLGHATA
                     LNAGIRRAAGPVVVVVDTSVEPAGDLVTPLVAALDDPSVAVAGPFGLVSDDLRTFEEP
                     PAGTVDVVAIEGYAQAFRRADYLPRGPLDEHFAYYRNLDIWWSLVLRDPFAADDGDDD
                     DGDGDGPEVIVDLGSMPRPRRAVCVSGVVLVRHEHRGWTALPEAERDRLSKRNFYRLL
                     KRFATRRDLLLEGAPLS"
     gene            complement(3428..>3830)
                     /locus_tag="AC162A_06405"
     CDS             complement(3428..>3830)
                     /locus_tag="AC162A_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015624623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme peroxidase"
                     /protein_id="HIR5010508.1"
                     /translation="MVKLASGQQMATKLNALGVSVTPLTKAEIIDGNGGASLDKLTST
                     EKDALAANTPLWFYILREAELNNGRLRGVGGRIVAETFHRAIEGSRFSILRDPTWHPT
                     LGRNATTFEMTDLLFFAYGGKKSEINPLGGN"
BASE COUNT          531 a         1422 c         1367 g          510 t
ORIGIN      
        1 gccgggcgtg aacaggcggc gccagctgtg gacgacgtcg ttggcggtga gcggcgtccc
       61 gtcgctgaac accaggtcat cccggagcgt gaacgtgacg cggcgcccgc cgtcctccac
      121 ggtccacgac tcggccaggg ccggccgaac gaccaaggac gggtcgatgg tggtgagggt
      181 ctcatagagc tggctgatgg tcgaggcgct gccgaggtcc ccgtgacgcg ccgggtcgat
      241 cgaggcgggt tcgccgttga cgatcgtgac cgtccccggg tccgcggccg cgaccgggtc
      301 cgggctttcg ccggcggcgg gccacaggcg gatcgccatc gcaaggacca cgaacaccgc
      361 gatcacgccc aggatcgccc tgcgctgacg cgatggcgcc gactcgggcc cggcaggccg
      421 gccccagtcc gacccgaccg gggggtcgaa cgtcgagaac gccaccggag gcggaggggg
      481 cgcgggcggc aactgggctg gaggcgcggg cggatgcgca gggatcggga ggtccgattt
      541 gcgttccgtc aggtcgtccg ccaccaggcc tcctccggca gggacggatc cggccgttgc
      601 gcgtcgagca gccgttccac cgggatcggg aggcgcggcc tcgctgacca cgcgtagcca
      661 ctgccggaga acccggtgag cagagccacg atgcagaccc agcgacgcac ggcgggagta
      721 tacgggcggg tcgccggaag cccccggaac ggccgccgta cgatggtcgg gtgaccggac
      781 ccgccctgcg taacacactg tcgcgccgcg tcgaacccgt gcgcgtcccg gacgacgcgc
      841 ccgtgcggat gtacacctgc gggccgacgg tctaccgctg ggcccacgtc ggcaacttgc
      901 gcagctacct gttcgcggac ctgatccggc ggacgctgct ctaccacggc atcccggtcc
      961 tccacgtgaa gaacatcacg gacgtcgggc acctgcgcga cgagcggttc gatcggggcg
     1021 aggaccggat gctggtcgcg gcgggcctcg agtcgaagac gaccgccgag atcgccgacg
     1081 cctacgaggc gggcttccac gcggacgagg cgctggtcaa catcctgccc gcgcacgtct
     1141 tcccgcgggc gaccgagcac atcgacgaca tgctcgagct cgccggacgc ctcgagtccc
     1201 tcggtcacgc ctatctctcg tccgccggca acctctacta cgccgtcgac tcgttccctg
     1261 ggtatggcgc cctgtcgggc aacacgctcg acagcctgcg cgccggccac cggcacgacg
     1321 tcgagcccga caagcgcgag cacgcggact tcgcgctgtg gaaggccgcg ggcgagggcc
     1381 ggatcctgcg ctggccgagc ccgtggggtg acggcttccc cggctggcac ctcgagtgct
     1441 ccgcgatggc cctccgctac ctgggccccg acttcgagat ccatacgggt ggcatcgaca
     1501 acatcttccc ccaccacgag gacgagatcg cccagtcgac gccgatcacc ggcggcccgc
     1561 cggtccgcca ctgggtgcac ggagagcacc tcctcgccgc cggccggaag atggcgaagt
     1621 cgaccggcaa cttcgagcgg gtcaccgagc tgcgcgaccg gggcatcgat cccctcgcct
     1681 tccgctatct caccctgacg gtccgctacg gccgcaagct caactactcg gacgcgtcga
     1741 tcgacgccgc ggcggccgca ctgtcgtcgc tgcgggcgca cctggccgcc ctgggtcccc
     1801 cacccgccga tggaccatgg gtggcgccgg ccgtcctccg ggccggtttc gcgggagcgc
     1861 ggccgcgcgg cgtcgccgat ggggtggccg gtcaccaggc cgggtcgccc ctcgcggccg
     1921 aatcgcgggc tttctcgatc gccgaccgtg ccggctcccc gaccgctccc ctgtccccgc
     1981 ccgggcgccg gctccacgac cggttcgttg cggcgatcga cgacgacctc gacctgccgg
     2041 ccgcgctcgc ggtcgtgcgc gagaccgtca ggactgacct gccatcgaac gagcggcgct
     2101 ggctcgtgct ggacgcggac ctggtgctcg ggcttgacct cgaccgggcg tgggaccccg
     2161 ccgccggggc tgcgaccgac acgggcgccg acgaccttcc ggccggggcg gcaccgatcc
     2221 tggaggcacg atccgcggcg cgggcggcgc gcgactgggc acgggcggac gccctgcgcg
     2281 accagctggc ggcgatgggc attgaggtgg tcgacggccc ggacgggacg acctggcacc
     2341 gccgccctag ctgagggggg cgccctccag cagcagatcg cggcgcgtcg cgaagcgctt
     2401 gagcagccga tagaagttcc gcttcgagag ccggtcgcgc tccgcctcgg gcaacgccgt
     2461 ccagccccgg tgctcgtgcc gcacgagcac gacgccgctg acgcacacgg cacgtcgggg
     2521 tcgcggcatc gaccccaggt ccacgatcac ctcgggcccg tcgccgtcgc catcgtcgtc
     2581 atcgccgtcg tcggccgcga acggatcgcg gaggacgagg ctccaccaga tgtcgaggtt
     2641 gcggtagtac gcgaagtgct cgtcgagcgg accccgcggg agatagtcgg cgcggcggaa
     2701 cgcctgcgcg tagccctcga tcgcgacgac atccaccgtg ccagcgggcg gctcctcgaa
     2761 cgtccggaga tcgtccgaga cgagcccgaa cggcccggca accgccaccg acgggtcgtc
     2821 gagggccgcc accagcggcg tgacgaggtc cccggcgggc tcgacgctgg tgtccacgac
     2881 cacgacgaca ggcccggcgg cgcgccggat cccggcgttg agcgccgtgg cgtggcccag
     2941 gcgcgtgctc gtccagacca cctcgagccc gacgccggtt gatccggtcc ccgctgcctc
     3001 gagctcggcc agggcgtccg actgggcctc ggacggaccg ttcgcgacga cgatgacctg
     3061 cgttcccgcc ggcgatcgcc ccgcgaatgc ctcgaccgtc cgcgacacgt ccgcgggcca
     3121 gtccgtcgcg accagcacga ccgacgcgaa cccgaccggc gcctcgtcga ggcgcgacgg
     3181 cacggacgcg ctggtgccat agcgcgtgac cccggcttcg acgaggtccg gcgccgcggc
     3241 ccgctccaac gagtacagcg aggccgcgtc gaggaccttc cagcccgcgg cctcgatctc
     3301 ggcccgcagc gcgtcggcgg tcgtccagtc cttcgcccgg cgggccgccg cgcgcgcgtc
     3361 ggcgagtgcg cgaacctcgg cgggcgcctc gtccttggcc atccccccga ggataccggg
     3421 agggccgtca gttgccgccg agcgggttga tctcggactt cttgccgccg taggcgaaga
     3481 acagcaggtc ggtcatctcg aaggtcgtgg cattgcgacc gagggtcggg tgccaggtcg
     3541 ggtcgcgcag gatcgagaac cggctgccct cgatcgcccg gtggaacgtc tcggcgacga
     3601 tgcgtccgcc caccccgcgc agccggccgt tgttcagctc cgcctcgcgc aggatgtaga
     3661 accacagcgg cgtgttggcg gcgagcgcgt ccttctccgt cgacgtcagc ttgtcgaggc
     3721 tcgcgccgcc gttgccgtcg atgatctcgg ccttggtgag cggcgtgacg ctgaccccga
     3781 gggcgttgag cttggtggcc atctgctggc cactcgccag cttcaccatc
//
LOCUS       DBNHXO010000303         3657 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3258948, whole genome shotgun sequence.
ACCESSION   DBNHXO010000303 DBNHXO010000000
VERSION     DBNHXO010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3258948"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="AC162A_06410"
     CDS             complement(<1..1190)
                     /locus_tag="AC162A_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5010509.1"
                     /translation="MADPLDKQLVEAVLASRPRRTKRELEEAKLASMRPRRADGVAVA
                     PVPGEAEDDDLDADEDIELSADEDAEPSTAELSVEIVTDEAVDAADEPVVPPGLDAPA
                     KLNAAEVEEPTAEELEALSADMIGIDDPVRMYLKEIGKVALLTAEEEVVLAKAIELGE
                     QIIIEPWKAVLSLHEWTLHDTERKTRTTKPQHSLPFGPETHKMVADALAAPDAAELFV
                     ASPDFHLIRAGKDVQSEGTTALLKEAKAKVAAYNAALDAATFQDILDWSYLSVHNGDL
                     DSRDNVGLRAIYDWSRDGVAFGALQRWIEGGNDSDLLRRMGFDPEVPPQTKMRDRKGD
                     VVKIGRDAREQLTSANLRLVVSIAKKYIGRGMSFLDLIQEGNIGLIRAVEKFDFEKGF
                     KFST"
     gene            complement(1238..3178)
                     /gene="dnaG"
                     /locus_tag="AC162A_06415"
     CDS             complement(1238..3178)
                     /gene="dnaG"
                     /locus_tag="AC162A_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR5010510.1"
                     /translation="MAVGTVAEIKGRLPVADVVGETVQLRKAGTTLKGLCPFHGEKTP
                     SFVVTPGRDTWHCFGCGLHGDIFTFVMERDGIAFPEALKVLAGRAGVEIDERSRREDA
                     HNARLREVMEGAIAFYHAVLLHSKPGEPALAYLRGRGFTDKTIEQQQLGWAPAGWDQL
                     VRQLASKRQVSPGELLEVGLASPRQRGNGVYDRFRERVIFPIRDANGHPVGLGGRVLG
                     REGVGDDGRDHGPKYLNSPATPLFDKSRTLYLIDRAKSAIRKTGQAVIVEGYTDALMA
                     HQAGFDNVVASLGTALTPGQVALITRYAKRIALAYDVDAAGQKAGTFGATALEELIRQ
                     LARDDSGVALDDVRVVQLPDGRDPDEILRDEPDRWREEVRTAKPIIDHLIDVHARAND
                     LRTPGGKTRFVDAVLNVLRGLADPVMRDAYLGQVQQVSGVEERTLLEAMHRRPVREPD
                     GRDGRDRFTAEAVTHAADALPVNDILRAVSRTEQELLRLVLLVPETHDAVLDGLGPDR
                     LPTQVARELYRAVVIARARDDHGVRPPYSLSAILDGLDEESRALGQALVSRPGPNPRE
                     LGDRAIAYEIERLTLELEDAEWQERSAFNRDAIAEAERDVDREAIDRLTLERRLLNEQ
                     RRSLDLRRDQTRLLARTPVAAG"
     gene            complement(3338..>3657)
                     /locus_tag="AC162A_06420"
     CDS             complement(3338..>3657)
                     /locus_tag="AC162A_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009192008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HIR5010511.1"
                     /translation="DVLEKIAEMPPSDRVMGERIHALVKASAPALTSRLWYGQPAYAR
                     DGKVVCFFQAAQKFKTRYATLGFSEEARLDDGTMWPTSYALTELTPADERRIGELVKQ
                     AAG"
BASE COUNT          469 a         1282 c         1311 g          595 t
ORIGIN      
        1 taggtcgaga acttgaagcc cttctcgaag tcgaacttct cgaccgcccg gatcaggccg
       61 atgttcccct cctggatcag gtccaggaac gacatcccgc ggccgatgta cttctttgcg
      121 atcgacacga cgaggcgcag gttggctgag gtcagctgct cccgggcgtc gcgcccgatc
      181 ttgaccacgt cgcccttgcg gtcgcgcatc ttggtctgcg ggggcacctc cggatcgaag
      241 cccatgcggc gcagcagatc ggagtcgttg ccgccctcga tccagcgctg gagcgcgccg
      301 aacgcgaccc cgtcccggga ccagtcgtag atggcgcgca gcccgacgtt gtcgcgcgaa
      361 tccaggtcgc cgttgtggac cgagaggtac gaccagtcga ggatgtcctg gaaggtcgcc
      421 gcgtcgagcg ccgcgttgta ggccgcgacc ttggccttcg cctccttgag cagcgccgtc
      481 gtgccctcgg actggacgtc cttgccggcc ctgatcaggt ggaagtcggg gctggcgacg
      541 aacagctccg cggcgtcggg ggccgcgagg gcgtcggcga ccatcttgtg ggtctccggc
      601 ccgaacggca ggctgtgctg cggcttggtc gtgcgggtct tgcgctcggt gtcgtgcagc
      661 gtccactcgt ggagcgagag gaccgccttc cagggctcga tgatgatctg ctcgccgagc
      721 tcgatcgcct tcgcaaggac gacctcctcc tcggccgtga gcagcgcgac cttgccgatc
      781 tccttgaggt acatccggac cgggtcgtcg atcccgatca tgtccgcgga cagcgcctcg
      841 agctcctcgg ccgtcggctc ctcgacctcg gcggcgttga gcttggccgg cgcgtcgagc
      901 cccgggggca cgacgggctc gtccgcggcg tcgaccgcct cgtcggtgac gatctcgacg
      961 ctcagctcgg ccgtgctcgg ctcggcgtcc tcgtcggcgg agagctcgat gtcctcgtcg
     1021 gcgtcgaggt cgtcgtcctc ggcttccccg ggcaccggcg ccacggccac gccatccgcg
     1081 cgccgcgggc gcatcgaggc aagcttcgcc tcctccagct cgcgcttggt gcggcggggc
     1141 cgcgacgcca gcacggcttc gacgagctgc ttgtccaagg ggtctgccat gtccgcgggt
     1201 cctccggtcg gttcggtcga ggagcgttcg agagtggtca gcccgccgca actggcgtgc
     1261 gagcgaggag tcgggtctgg tcgcggcgca ggtccagcga ccggcgctgt tcgttgagga
     1321 ggcgacgctc gagcgtcagg cggtcgatgg cctcgcggtc gacgtcccgc tcggcctcgg
     1381 cgatggcgtc gcggttgaac gcgctgcgct cctgccactc ggcgtcctcg agctcgagcg
     1441 tgaggcgctc gatctcgtag gcgatggccc ggtcgcccag ctcgcgcggg ttgggtcccg
     1501 ggcgcgagac gagcgcctgc ccaagggccc ggctctcctc gtcgagcccg tcgaggatcg
     1561 ccgagaggga atacggcggg cgcaccccgt ggtcgtcgcg ggcccgggcg atgacgacgg
     1621 cgcggtagag ctcacgggcg acctgcgtcg gcagccggtc cggcccgagg ccgtcgagca
     1681 ccgcgtcgtg cgtctcgggc accagcagca cgaggcggag cagctcctgc tccgtgcgcg
     1741 agaccgcgcg caggatgtcg ttgacaggca gggcgtcggc ggcgtgggtg acggcctccg
     1801 cggtgaaccg gtcgcggcca tcgcggccgt cgggctcacg gacaggccgg cggtgcatcg
     1861 cctcgagcag cgtccgctcc tccaccccgg acacctgctg cacctgcccg aggtaggcgt
     1921 cacgcatgac gggatccgca aggccgcgaa ggacgttgag cacggcatcg acgaaacgcg
     1981 tcttgccgcc gggggtgcgc aggtcgttgg cgcgggcgtg gacgtcgatc aggtggtcga
     2041 tgatcggctt cgccgtccga acctcctcgc gccagcggtc cggctcgtcg cgcaggatct
     2101 cgtcggggtc gcggccatcg ggcagctgca cgacgcggac gtcgtcgagc gcgacccccg
     2161 agtcgtcgcg ggcgagctgc cggatcagct cctcgagcgc ggtcgccccg aaggtgccgg
     2221 ccttctggcc tgcggcgtcg acgtcgtagg cgagcgcgat ccgcttcgcg tagcgggtga
     2281 tcagggcgac ctggccgggc gtcagcgcgg tgcccagcga ggcgacgacg ttgtcgaagc
     2341 ccgcctggtg tgccatcagc gcgtccgtgt agccctcgac gatcaccgcc tggccggtct
     2401 tgcggatcgc cgacttggcg cggtcgatca ggtacagcgt ccggctcttg tcgaacagcg
     2461 gggtcgccgg cgagttgagg tacttcggcc cgtggtcgcg cccgtcgtcg cccacgcctt
     2521 ccctgcccag cacgcgcccg ccgagcccca ccgggtgccc gttcgcatcc cggatcggga
     2581 agatcacccg ctcacggaac cgatcgtaga cgccgttgcc tcgctggcgt gggctcgcca
     2641 gcccgacctc gagcagctcg ccggggctga cctgccgctt cgacgcgagc tggcggacca
     2701 gctggtccca gcccgcaggc gcccagccca gctgctgctg ctcgatggtc ttgtcggtga
     2761 agccgcggcc acgcaggtag gcgagggctg gctcgcccgg cttcgagtgc agcaggaccg
     2821 cgtggtagaa ggcgatcgcg ccctccatga cctcgcgcag ccgcgcgttg tgcgcgtcct
     2881 cgcgccgcga gcgctcgtcg atctcgaccc cggcgcggcc ggccaggacc ttgagggcct
     2941 cggggaaggc gatgccgtcc cgctccatca cgaacgtgaa gatgtcgccg tgcaggccgc
     3001 agccgaagca gtgccacgtg tcgcggcccg gggtcacgac gaagctgggc gtcttctcgc
     3061 cgtggaatgg gcacaggccc ttgagggtgg tcccggcctt ccgcagctgg acggtctcgc
     3121 cgacgacgtc ggcgaccggg aggcggccct tgatctcggc gacggtaccg acggccaacg
     3181 ctgctcctgt cattcacgct ccgccgggcg tcacgcggcg gacatgactg cgggcctgtc
     3241 aagtcttgac aggccgcgat cggggcaggg tacgcccggc tttggggctt gacaagcgat
     3301 tcttgtcaag tcccccgcag cgtggcgagc tgggtcgtca gcccgccgcc tgcttcacca
     3361 gctcgccgat ccgtcgctcg tcggcagggg tcagctcggt caaggcgtag gaggtcggcc
     3421 acatggtgcc gtcgtcgaga cgggcctcct cgctgaaacc gagcgtcgcg tagcgcgtct
     3481 tgaacttctg cgcggcctgg aagaagcaga cgaccttccc gtccctggca tacgccggct
     3541 gcccgtacca gagcctcgac gtgagggctg gcgcgctggc cttgacgagc gcgtggatcc
     3601 gctcgcccat cactcgatcc gacggcggca tctcggcgat cttctcgagc acgtcgc
//
LOCUS       DBNHXO010000304         2395 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2466813, whole genome shotgun sequence.
ACCESSION   DBNHXO010000304 DBNHXO010000000
VERSION     DBNHXO010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2466813"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="AC162A_06425"
     CDS             complement(<1..466)
                     /locus_tag="AC162A_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014515618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR5010512.1"
                     /translation="MREAWIVDAVRTPIGRYGGALASVRPDDLAAVVIAAIVERTGID
                     AAAIEDVVFGCANGAGEDNRNVARMALLLAGLPVEVAGQTVNRLCGSGLQAVNTAAHA
                     IGAGDGEVFVAGGVESMTRAPYVMLKAEGAWDRGPRTVEDTTLGWRFVNPRMQ"
     gene            542..1450
                     /locus_tag="AC162A_06430"
     CDS             542..1450
                     /locus_tag="AC162A_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010513.1"
                     /translation="MSQRARLGRTAPITALTLVAASLVAACGGVAPATQVVSPPAAAS
                     PGSATPSPTASASLPADAIAHPTGPNDVVLRAGTRGGFVRLQTVMSRVPEFTLYGDGR
                     ALVLPPDGDAADDALPALRETRLSEDEVQALLAYALLEGGLGTARDAYMGGNMDAPST
                     VFDLHADGADRSILVTGLTDDPAPGPDAAAMRAFADLVAKLRSIPTDADYASDRTIAF
                     IAETEGAQGPVEAWPWTDLAPADFAQPADDAAIPFPSHILDPEQVEAAASLAGGQPAL
                     VGVKGPDGRTYSLLLRPALPDEVPGG"
     gene            complement(1455..2252)
                     /locus_tag="AC162A_06435"
     CDS             complement(1455..2252)
                     /locus_tag="AC162A_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5010514.1"
                     /translation="MRTDVPMARIAANGLDIGYDVDGEGPPLVLLHSASSSGREDFAA
                     QLPMLRRTFACYLPDARGHATTRWDAADGFRYDWLVDDLEAFTDALGLDTFHLLGFSM
                     GAATALHFAARSPERLRSLVVIGISPRREPRASVARRMMDPAFILRDPAWAAELSRRH
                     DPVQGAGAWERLMPAIARDVAEQRLLVPTELIRIDAPTLVVVGDRDTFVPVDQALGLK
                     RQLPDARLFVAPDCGHEVPARRAALFNEALAGFYRSTAAVAEKRAEG"
BASE COUNT          321 a          955 c          823 g          296 t
ORIGIN      
        1 cctgcatccg cgggttcacg aatcgccagc cgagggtcgt gtcctcgacc gttcgcggcc
       61 cgcggtccca ggcgccctcg gccttgagca tcacgtacgg ggcccgcgtc atcgactcga
      121 ccccaccggc cacgaacacc tcgccgtcgc ccgccccgat cgcgtgcgcc gccgtgttga
      181 cggcctgcag ccccgaaccg cacaagcggt tcaccgtctg gcccgcaacc tcgacgggca
      241 gccccgcgag caggagcgcc atgcgcgcca cgttgcggtt gtcctcgccg gcgccattgg
      301 cgcagccgaa cacgacgtcc tcgatggcgg cggcgtcgat gcccgtccgt tcgacgatcg
      361 cggcaatcac gaccgccgcg aggtcatcgg gccgtaccga cgccagcgcg ccgccgtagc
      421 ggccgatcgg ggtccggacc gcgtcgacga tccatgcctc gcgcaggggc cgctgcaggt
      481 cacgcaccat ggaccgattg tggcatggga accgagcgca cgatcccggc gtccgggcgc
      541 catgtcccaa cgcgcccgac tcggccggac cgctcccatc acggcactca cgctcgtcgc
      601 cgcctcgctc gtcgcggcgt gcggcggggt cgcgccggcg acgcaggtgg tgtcgccccc
      661 ggccgccgct tcgcccggct cagccacgcc gtccccgacg gcctccgcat cgctcccggc
      721 ggacgccatc gcccacccga ccggccccaa cgacgtggtc ctgcgcgccg ggacccgcgg
      781 cggcttcgtc cgcctgcaaa cggtcatgag ccgtgtcccg gagttcaccc tctacggcga
      841 cgggcgcgcc ctcgtgctcc cgccggacgg cgatgccgcg gacgacgccc tgccggcact
      901 ccgcgagacc cgtctctcgg aggacgaggt ccaggcactc ctcgcctacg cgctgctcga
      961 gggcgggctg ggaaccgcgc gcgacgccta catgggcggc aacatggacg cgccctcgac
     1021 cgtgttcgac ctccacgccg acggcgccga ccgctcgatc ctcgtgactg gcttgaccga
     1081 cgatccggcg cccggcccgg acgccgccgc gatgcgggcg ttcgccgatc tcgtcgcgaa
     1141 gcttcggtcg atcccgaccg acgccgacta cgcgtccgac cgaaccatcg cgttcatcgc
     1201 cgagaccgag ggcgcgcagg gccccgtcga ggcgtggccc tggaccgatc tcgcgccggc
     1261 cgacttcgcc cagccggcgg acgacgcggc gatcccgttc ccgtcgcaca tcctcgaccc
     1321 ggagcaggtc gaggcggccg cctcgctcgc cggcggtcag cccgccctgg tcggcgtgaa
     1381 ggggcccgac gggcgcacct acagcctgct ccttcgcccg gccctccccg acgaggtccc
     1441 gggcggctga ccggctagcc ctccgcccgc ttctccgcca cggcagccgt cgaccggtag
     1501 aagcccgcca gcgcctcgtt gaacaacgcg gcccgcctgg ccggcacctc gtggccgcag
     1561 tcgggcgcga cgaacagccg ggcatcaggc agctggcgct tgaggcccag ggcctggtca
     1621 acggggacga acgtatcccg atcgccaacg acgaccagcg tcggggcgtc gatccggatc
     1681 agctccgtcg gcacgagcaa ccgctgctcc gcgacgtcgc gggcgatcgc cggcatcagc
     1741 cgctcccagg ctcccgcgcc ctggaccggg tcgtggcggc gcgagagctc ggccgcccac
     1801 gcggggtccc gcaggatgaa cgccgggtcc atcatccggc gcgcgaccga ggcccggggc
     1861 tcacgccgcg gcgagatccc gatcacgacg agcgatcgga gccgttccgg cgagcgcgcc
     1921 gcaaagtgga gcgccgtggc cgcgcccatc gagaacccga gcaggtggaa cgtgtccagg
     1981 ccgagcgcgt ccgtgaaggc ctcgaggtcg tcgacgagcc agtcgtagcg gaacccgtcc
     2041 gcggcgtccc agcgggtcgt cgcgtggccg cgggcgtcgg gcaggtagca ggcgaacgtc
     2101 cggcggagca tcggcagctg ggcggcgaag tcctcgcgtc cggacgagct ggcgctgtgg
     2161 agcaggacga ggggcgggcc ctccccgtca acgtcgtagc cgatgtccag cccgttcgcg
     2221 gcaatccgtg ccatcgggac gtccgtgcgc atggccgcca gcatatccac ggcccgcgtg
     2281 cgtcccggcc gcgccgtacc atcgtcgcca tgcaagccga tcggtccggc ggcggggccg
     2341 ccacccccgc cacccgcgtc gagcgcgact cgatgggcga gatggacgtt cccgc
//
LOCUS       DBNHXO010000305         1580 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4054133, whole genome shotgun sequence.
ACCESSION   DBNHXO010000305 DBNHXO010000000
VERSION     DBNHXO010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4054133"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="AC162A_06440"
     CDS             <1..653
                     /locus_tag="AC162A_06440"
                     /inference="COORDINATES: protein motif:HMM:NF016202.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR5010515.1"
                     /translation="WLPARIIDKTVLYPGQRFEWWSALGPVTSSRGFGPGGFIAGNHT
                     EPTGALGGGMCSSSTTLFNAALRAGLRMGARSNHKYYIYRYPLGLDATVSKLAGGGGQ
                     TMSFTNDMKHPIVIRSYRYTAGGLGWVRYEIWGIPDGRRVSISKPSVANRVQATTRTE
                     YVSTLPRGVKEQTEYPADQMDVSVTRVVRNRNGGIIHRDIWRTHYVLWNGVVQIGR"
     gene            724..>1580
                     /locus_tag="AC162A_06445"
     CDS             724..>1580
                     /locus_tag="AC162A_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR5010516.1"
                     /translation="MPGIIPRWEWRTFGSRYGIAESRFAALTPTGVQESDELYLVGGA
                     GDNVKVRDDLMDIKVLRETDADGLERWEPVMKAAFPLPAADVGRVFESLRLPAPDLAR
                     EAYTLDQFVDELVAPSGTLRAVKVHKRRVRYKVGGCTSEVSDVTVDGIASRTIAIESE
                     DAAAVVAAVRSVGLDGFHNMSYPVGLRAVLEGQPERYAVLDVGTNSIKFYVGERRAGG
                     GWTRVVDRAEITRLGEGLRENGRISPEAQERSITAITGMVDEATREGARAIVGVATAG
                     LRMAANSDDV"
BASE COUNT          253 a          539 c          558 g          230 t
ORIGIN      
        1 tctggctgcc ggccaggatc atcgacaaga ccgtcctgta cccgggtcag cgcttcgagt
       61 ggtggagcgc cctggggccc gtgacctcga gccgcgggtt cggtccgggc gggttcatcg
      121 cgggcaatca caccgagccg accggcgccc tcggcggcgg gatgtgctcg agctcgacga
      181 ccctgttcaa cgccgcgctc cgcgccgggc tgcgcatggg cgcccgctcg aatcacaagt
      241 actacatcta ccgctacccg ctgggcctcg acgcgaccgt ctcgaagctc gccggcggcg
      301 gtggccagac gatgtcgttc accaacgaca tgaagcaccc gatcgtgatc cgcagctacc
      361 gctacaccgc tggtggcctc ggctgggtcc gctacgagat ctggggcatc cccgacggac
      421 ggcgggtcag catctccaag ccgtcggtcg ccaaccgcgt ccaggcgacg acgaggaccg
      481 aatacgtcag cacgctcccg cgcggcgtca aggagcagac cgagtacccc gcggaccaga
      541 tggacgtctc ggtcacccgc gtcgtgcgca atcgcaacgg gggcatcatc caccgggaca
      601 tctggcgcac ccactacgtg ctctggaacg gcgtcgtcca gatcggacgc tgacgggccc
      661 cggcccggca gtagactggc cgggccgccg gggtgatccc ggtcgatctg gatcgagggc
      721 gagatgccgg ggatcatccc gcgctgggaa tggcggacgt tcgggagccg ctacggaatc
      781 gctgagtcga ggttcgctgc gctgacgccc acgggcgtgc aggaaagtga cgagctgtac
      841 ctcgtcggcg gcgccggcga caacgtgaag gtccgcgacg acctcatgga catcaaggtc
      901 ctgcgcgaga ccgacgcgga tggcctcgag cgctgggagc cggtcatgaa ggcagccttt
      961 ccgctgcctg ccgccgatgt cggccgggtg ttcgagtcgc tccgcctgcc cgcgcccgac
     1021 ctcgctcgag aggcgtacac gctcgaccag ttcgtcgacg agctcgtggc accgtcgggc
     1081 acgctccgcg cggtgaaggt gcacaagcgc cgcgtccgct acaaggtcgg cggctgcacc
     1141 tccgaggtgt ccgacgtcac cgtcgacggc atcgcgagcc gcacgatcgc gatcgagtcc
     1201 gaggatgcgg ccgcggtcgt ggctgccgtg cgctcggtgg gcctcgacgg gttccacaac
     1261 atgagctatc cggtcggcct gcgggcggtc ctcgaggggc agccggagcg atatgcggtg
     1321 ctcgacgtcg ggaccaactc gatcaagttc tacgtcggcg agcgtcgcgc gggtggcggc
     1381 tggacacgtg tcgttgaccg ggccgagatc acccgcctgg gcgagggact ccgcgagaac
     1441 ggccggatct cgccggaggc acaggaacga tcaatcaccg cgatcaccgg gatggttgac
     1501 gaggcgacgc gcgagggcgc ccgcgccatc gtgggagtcg caacggccgg ccttcggatg
     1561 gccgccaact cggacgacgt
//
LOCUS       DBNHXO010000306         5370 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4253577, whole genome shotgun sequence.
ACCESSION   DBNHXO010000306 DBNHXO010000000
VERSION     DBNHXO010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4253577"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..149
                     /locus_tag="AC162A_06450"
     CDS             3..149
                     /locus_tag="AC162A_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR5010517.1"
                     /translation="MGDFAAVNAVYASHMPDPPPARSTFGVGALPKAALVEIEMIAGR
                     PAGR"
     gene            219..722
                     /locus_tag="AC162A_06455"
     CDS             219..722
                     /locus_tag="AC162A_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5010518.1"
                     /translation="MTAHRLDPEQRADLMARYRAGHGAVVAAVEGITDAELDTRPPGE
                     GEWTPREIVHHLADSEMTSAIRVRRLIAEELPVIAGYDEAEWAQRLHYDDRPIGPSLD
                     ALAAARSTTADLLDHLTDAEWGRAGTHTDSGAYDVERWLEIYAAHAHDHAAQIRRARG
                     AADGGVG"
     gene            849..2306
                     /gene="glmU"
                     /locus_tag="AC162A_06460"
     CDS             849..2306
                     /gene="glmU"
                     /locus_tag="AC162A_06460"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR5010519.1"
                     /translation="MTPSSPGTPVRRTVAIILAAGLGTRMKSGVPKVLHPVCGRPMID
                     YVLDAAIGATGSRPVVVYSPATAPVREAVESRADTALQDEPRGTGDALRAALAVVPDD
                     AEEVLVLSGDVPLVAQDQLATLLELRGLDHAAVALVSVDAIDPTGLGRVVRNDGGTVE
                     RIVEDKDATDEERQISEINAGLYAIDATWLRRRIGDLKPSAATGELYLTDLVAFARED
                     GRIVAALDTDDDGRLTGINDRSQLARAEWDMRVDLNDRWMRAGVTMLDPSTVYLDHDV
                     DLAQDVVLEPNVILRGKTSVGERTRIATGSQIFASSIGTDCVIWASVIERSVVEDHVT
                     IGPFSHLRPGTHVGARSEIGNFAELKNSTLGEHVRQHHNSYLGDADVGADTNVGAGTI
                     TANYDGVSKHRTTIGERVFLGVDTMLRAPVTLGDGSKTGAGAVVTRDVPPGKLAVGVP
                     ARIREPRAPREEPQAPSDPVDPVEDRAAEVTPADG"
     gene            2299..3636
                     /locus_tag="AC162A_06465"
     CDS             2299..3636
                     /locus_tag="AC162A_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR5010520.1"
                     /translation="MGNTFVELGIIALLVLINGIFVAAEIALVSIRPSRVEQMAEEGR
                     RGAKRVRKLTNDPGRFLAVIQIAITFIGFLASAYAGVSLADSLRDFFVQLGMPASYAG
                     VFGLLIVTILLSLFTIVFGELVPKTLALAHPEAFALRLSQPVDVLGRVLRPLVAFLTA
                     TTRTIARAFGADVTAQAQITADELRLIVERGGEQGILEAEEEQMINAVIELGNRRLHE
                     VMVPRTAIAALPASATLDEVIDKVIEEGHSRVPIYETSVDEVLGILYAKDLLPFLKSE
                     ISERPAMRTLLRTPVFVPESMSIDDLLHEFQRRKVHIAIVLDEYGGTAGLVTIEDLLE
                     EIVGEIQDEYDVEEPLIEELGEDRVRVDGRASVDDLLELWDLRLQLEDDDEYDTVGGL
                     VYHRIGGVPSPGDEVHLDGLRLTVETTDGRRVGKVLVVRERRVDDDSVDDDEE"
     gene            complement(3655..4677)
                     /locus_tag="AC162A_06470"
     CDS             complement(3655..4677)
                     /locus_tag="AC162A_06470"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR5010521.1"
                     /translation="MAITRDIDLSLLLADTEDEIMRVVADRDASTAGVYEMIRYHLGL
                     DGDDGPRGKRMRPLLGLLAYASITGDHRAALPGAAAVELGHNFSLVHDDIEDGDIERR
                     HRATLWALHGVPQAINTGDLIFSLSRVALHRLTDLGFSDTKVLRLMRLYDETCVLLCE
                     GQYLDIASSASDEPMSVELYFDMIGRKTAALIAASIEAGALLATDDEDVISGYRRFGW
                     SLGIAFQVNDDLLGIWGEQRQTGKEATDVARHKKTLPVLYGLQHGSPEDRARLSALYV
                     KADPDGGDVAEIVRILERSGAREYTRTEARRHRDAALAELADLSVVDRAARDELTGII
                     ESVIRA"
     gene            4796..>5370
                     /gene="recO"
                     /locus_tag="AC162A_06475"
     CDS             4796..>5370
                     /gene="recO"
                     /locus_tag="AC162A_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR5010522.1"
                     /translation="MPAPRRYVTDAIVLSRFDLGEADRVLTLITPHDGKLKAIAKGIR
                     RPASRIGGSLEPFAELRVTLARGRTFDVVTEVHVGHAWLALRDHLESTATAWYLAELA
                     DRSLEERHAAEPLYALLHRAYELLDAGMAPGRVARWYEMHLADELGQRPEVDRCVECD
                     RMLEATETFRWVPPLGGVLCSRCPGPPADRTA"
BASE COUNT          787 a         1908 c         1929 g          746 t
ORIGIN      
        1 acatcggcga cttcgcggcc gtgaacgcgg tctacgcgag ccacatgccc gacccgccgc
       61 ccgcccgctc gacgttcggc gtcggggccc tgcccaaggc ggcgctcgtg gagatcgaga
      121 tgatcgccgg ccgccccgcg ggccgctgag gcgacgaccc accctatcct tggcggagcg
      181 accctgcctc gcccgcccag gcccaccgga gtgccccaat gaccgcccat cgcctcgacc
      241 ccgagcagcg cgccgacctg atggcccggt accgcgcggg ccatggcgcc gtggtggccg
      301 cggtcgaggg catcaccgac gcggagctgg acacccggcc gcccggtgag ggcgagtgga
      361 cgccccgcga gatcgtccac cacctcgctg acagcgagat gacctccgcg atccgcgtgc
      421 ggcgcctcat cgccgaagag ctgcccgtga tcgccgggta cgacgaggcg gagtgggcgc
      481 agcgcctcca ttacgacgac cgcccgatcg ggccgtccct ggacgcgctc gcggcggctc
      541 gctcgacgac cgccgacctc ctggaccacc tcaccgacgc ggagtggggc cgcgccggaa
      601 cgcacacgga cagcggggcg tacgacgtgg aacgctggct cgagatctac gcggcccacg
      661 cgcacgacca cgcggcccag atccggcggg cccgcggcgc ggcggacggc ggcgtcggtt
      721 gacactccca cggacccgcc cgcagaatcc gcgccactcc tgatgaccaa ttcgctcacc
      781 tcctcgggcg ccggccccgc cgctccacct gcggcgttcc ggtcgcgaac gcgcgggcgc
      841 ccgaccgtat gaccccctca agtccaggta cgccggtccg gcgcacggtc gcgatcatcc
      901 tcgcggccgg cctcggcacg cgcatgaagt cgggcgtgcc caaggtcctc cacccggtct
      961 gcggccgtcc gatgatcgac tacgtgctcg acgccgcgat cggcgcgacg gggagccggc
     1021 cggtcgtcgt ctactcgccc gcgaccgccc cggtccgcga agccgtcgag tcgcgggccg
     1081 acaccgcgct ccaggacgag ccacgcggga ccggcgatgc cctgcgggcg gcgctggccg
     1141 tggtgccgga cgacgccgag gaggtgctcg tcctctcggg cgatgtgccg ctcgtcgcgc
     1201 aggaccagct ggcgacgctc ctcgagctcc gcgggctgga ccatgcggcc gtcgcgctgg
     1261 tctcggtcga cgcgatcgat ccgacggggc tcgggcgggt cgtgcgcaac gacggcggga
     1321 ccgtcgaacg catcgtggag gacaaggacg cgaccgacga ggaacgccag atctcggaga
     1381 tcaacgcggg gttgtacgcg atcgacgcga cctggctgcg gcggcggatc ggcgacctga
     1441 agccgagcgc ggcgaccggc gagctgtacc tcacggacct cgtcgcgttc gcgcgcgaag
     1501 acgggcggat cgtggccgcg ctggacaccg acgacgacgg ccgcctgacc gggatcaacg
     1561 accgttcgca gctcgcgcgc gccgagtggg acatgcgcgt cgacctcaac gaccgctgga
     1621 tgcgggccgg cgtgaccatg ctcgacccct cgacggtgta cctcgaccat gacgtggacc
     1681 tggcccagga cgtcgtgctc gagcccaacg tgatcctgcg cggcaagacg tcggtcgggg
     1741 agcggacgcg gatcgcgacg ggctcgcaga tcttcgcgtc gagcatcggc accgactgcg
     1801 tgatctgggc gagcgtcatc gagcgctcgg tcgtcgagga tcacgtcacg atcggcccgt
     1861 tcagccacct gcgcccgggc acccatgtgg gagcgcggtc cgagatcggc aacttcgccg
     1921 agctcaagaa cagcaccctc ggcgagcacg tgcggcagca ccacaacagc tacctcggcg
     1981 acgcggacgt gggcgccgac acgaacgtcg gcgcgggcac gatcacggcg aactacgacg
     2041 gcgtgagcaa gcaccggacc acgatcgggg agcgggtctt cctgggtgtc gacacgatgc
     2101 tgcgggcgcc ggtcacgctg ggcgacgggt cgaagaccgg tgccggggcc gtcgtcacca
     2161 gggacgtccc gccgggcaag ctcgccgtcg gcgttccggc ccggatccgc gagccccgtg
     2221 caccgcgcga agagccgcaa gctccgtcgg atccggtcga cccggtggag gacagggccg
     2281 ccgaagtgac ccccgccgat gggtaacacg ttcgtcgagc tcgggatcat cgcgctgctc
     2341 gtcctgatca acggcatctt cgtggccgcc gagatcgccc tcgtctcgat ccgccccagc
     2401 cgggtcgagc agatggccga ggagggccgg cgcggcgcga agcgggttcg caagctgacc
     2461 aacgaccccg gtcggttcct cgcggtcatc cagatcgcga tcacgttcat cgggttcctg
     2521 gcgtcggcgt atgccggggt gagcctggcc gactcgctcc gcgacttctt cgtccagctg
     2581 ggcatgcccg ccagctacgc gggcgtgttc ggcctgctga tcgtgacgat cctgctctcg
     2641 ctgttcacga tcgtgttcgg tgagctcgtg ccgaagacgc tggcgctggc ccaccccgag
     2701 gcgttcgcgc tccgtctgtc gcaacccgtc gacgtgctgg ggcgcgtgct gcggccgctc
     2761 gtcgcgttcc tgacggctac cacgcggacg atcgcccgag ccttcggggc agacgtcacc
     2821 gcgcaggcgc agatcaccgc ggacgagctg cgcctgatcg tcgaacgggg cggcgagcag
     2881 gggatcctcg aggcggagga agagcagatg atcaatgcgg tcatcgagct gggcaatcga
     2941 cgcctgcacg aggtgatggt gccgcgcacc gcgatcgcgg ccctgccggc atcggcgacc
     3001 ctggacgagg tgatcgacaa ggtcatcgag gaggggcact cccgggtgcc gatctacgag
     3061 acgtcggtcg acgaggtgct cgggatcctg tacgcgaagg acctcctgcc gttcctcaag
     3121 tccgagatct ccgagcggcc cgccatgcgg acactcctgc gcacgcccgt gttcgtgccc
     3181 gagtcgatgt cgatcgacga cctcctccac gagttccagc ggcgcaaggt ccacatcgcg
     3241 atcgtgctcg acgagtacgg cggcaccgcc ggcctggtga cgatcgagga cctgctcgag
     3301 gagatcgtgg gtgagatcca ggacgagtac gacgtcgagg agccgctgat cgaggagctg
     3361 ggcgaggacc gggttcgcgt cgacgggcgg gcgtcggtcg acgacctgct cgagctgtgg
     3421 gacctgcgcc tccagctcga ggacgacgac gagtacgaca cggtcggcgg gctcgtgtac
     3481 caccggatcg gcggggtccc gtcgccgggc gacgaggtcc acctcgacgg cctgcgcctg
     3541 acggtcgaga cgaccgacgg ccggcgggtg ggcaaggtgc tggtcgtccg cgagcgcagg
     3601 gtcgacgacg attcggtgga cgacgacgag gagtagcccg gcgccggccg gcggctaggc
     3661 gcggatgacg gactcgatga tgccggtcaa ctcgtcccgg gcggcccggt cgacgacgga
     3721 caggtccgcg agctccgcga gggcggcgtc gcggtggcgg cgggcctcgg ttcgggtgta
     3781 ctcgcgggcg cccgagcgtt ccaggatgcg cacgatctcg gccacgtcgc cgccgtcggg
     3841 atcggccttc acgtacaggg ccgacaggcg ggcccgatcc tcggggctgc cgtgctggag
     3901 cccgtacagc accgggaggg tcttcttgtg ccgcgcgacg tcggtcgcct ccttgcccgt
     3961 ctggcgctgc tcgccccaga ttccgagcag gtcgtcgttg acctggaacg cgattccgag
     4021 cgaccagccg aagcggcgat accccgagat cacgtcctcg tcgtccgtcg ccagcagcgc
     4081 cccggcctcg atcgacgccg cgatcagcgc cgccgtcttg cggccgatca tgtcgaagta
     4141 gagctcgacc gacatcggct cgtccgaggc cgagctggcg atgtcgaggt actggccctc
     4201 gcacaggagc acgcacgttt cgtcgtacag gcgcatcagc cgcagcacct tcgtgtccga
     4261 gaacccgagg tccgtcaggc gatggagggc gacgcgcgag aggctgaaga tgaggtcgcc
     4321 cgtgttgatc gcctgcggca cgccatggag tgcccacagg gtcgcgcggt ggcggcgctc
     4381 gatgtcgccg tcctcgatgt cgtcgtggac gaggctgaag ttgtggccga gctccaccgc
     4441 ggcggcgcca ggcagggccg cgcggtggtc gcccgtgatc gaggcgtagg ccaggagccc
     4501 gagcagggga cgcattcgct tgccgcgcgg gccgtcgtcg ccgtcgagcc cgaggtggta
     4561 gcggatcatc tcgtagacgc ccgccgtcga tgcgtcgcgg tccgcgacga cccgcatgat
     4621 ctcgtcctcg gtgtcggcga ggaggaggga gaggtcgatg tcgcgcgtga tggccatggc
     4681 ccccgcagtg tagcggctcc ggacgcaaac gtgtccgtta aaagtagcct gttggttgca
     4741 gggcggcaac gcgggcatcg gccggttcac gacgggcgag tggtaccgtc gagaggtgcc
     4801 cgcaccccgt cgttacgtga ccgacgcgat cgtcctctcg aggttcgacc tgggcgaggc
     4861 cgaccgcgtg ctgaccctga tcacgcccca cgacggcaag ctcaaggcca tcgccaaggg
     4921 catccgccgg ccggcgtcac gcatcggcgg gtcgctcgag ccgttcgccg agctgcgcgt
     4981 cacgctggcc cgtggccgga ccttcgacgt cgtgacggag gtccacgtcg ggcacgcctg
     5041 gctcgcgctc cgcgaccacc tcgagagcac cgcgaccgcc tggtacctcg ccgagctcgc
     5101 cgaccggtcg ctcgaggagc gccacgccgc cgagccgctg tacgcgctgc tgcaccgggc
     5161 ctacgagctg ctcgacgccg gcatggcccc gggccgcgtc gcccgctggt acgagatgca
     5221 cctcgccgac gagctcgggc aacgccccga ggtcgaccgc tgcgtcgagt gcgaccggat
     5281 gctcgaggcc accgagacgt tccgctgggt cccgccgctg ggcggcgtgc tgtgcagccg
     5341 ctgccccggt ccacccgcgg atcggaccgc
//
LOCUS       DBNHXO010000307         5829 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3260948, whole genome shotgun sequence.
ACCESSION   DBNHXO010000307 DBNHXO010000000
VERSION     DBNHXO010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3260948"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..764)
                     /locus_tag="AC162A_06480"
     CDS             complement(231..764)
                     /locus_tag="AC162A_06480"
                     /inference="COORDINATES: protein motif:HMM:NF015957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR5010523.1"
                     /translation="MTDRLYRSPTDRVIAGVAGGLATWLRVDPSIVRVAWVVLAVFSG
                     GIFLLVYIVMMIVVPLPPAGWVPSVGPPPQAGGTWTPPPSGGQWTPPPGGGSWTPPPS
                     GSGSWTPPQGSVPSWKAPDPTNAGIVFGFILVGLGVWFLVAQYIHIDWQLVWPVLVIV
                     IGAALIVGAMRRNRPTT"
     gene            complement(757..1737)
                     /locus_tag="AC162A_06485"
     CDS             complement(757..1737)
                     /locus_tag="AC162A_06485"
                     /inference="COORDINATES: protein motif:HMM:NF046770.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF transmembrane domain-containing protein"
                     /protein_id="HIR5010524.1"
                     /translation="MHINRGMLGWGVFLIVAGSVPLLVQAGAVDPEVIRRVWQLWPLI
                     LIGIGLGLILQRTRAAVVGGLIVAVTCGLLAGGWFAVGFSPGAGFGTCGLGGDAQGDP
                     FPSQSGTLATTASVDLDISCGDAVIATAAGSGWSVSGTSDGGIPPTITSGQDLKVASP
                     HRSGIDLGHASSWQVTLPADVSIHLGVSANAGTVRADLGSTQVPNLSASVNAGSATID
                     MGDVTGLQTLDVSANAGSLSLTLPTPTGTVNGSISANAGSVELCVPDGVGLRITSSTS
                     LGSNNFESRGLAHVGDTWVRPGLETSQQEIALSVSANLGNVTLNPEGGCD"
     gene            1842..2510
                     /locus_tag="AC162A_06490"
     CDS             1842..2510
                     /locus_tag="AC162A_06490"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5010525.1"
                     /translation="MSEPRRFDAVLFDLDGVLVDSEPWWNDVRIAFAGAHGRRWTDDD
                     HHAVMGANSRGWAITMRDRLELDGMNLDTIQDAVVAGVVERYRTEPPPVIPGATEAVR
                     RIAAQVPVAIASSAHPDVIAAAIDALGLRDVLGAIVSSDDVPHGKPSPDVYLAAASQL
                     GVEPARCLVVEDSVNGVKAGKAAGMFVVLVPNESVPPRPGTADLADLVIERLADLDPG
                     RVAA"
     gene            2543..3319
                     /locus_tag="AC162A_06495"
     CDS             2543..3319
                     /locus_tag="AC162A_06495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5010526.1"
                     /translation="MATPLTAGPVYRPIRVRIARLISAILVRSVVRLRFEGRDRLPGG
                     PAIYVSNHLNWADPVVLLAVLPGRPKVALFGPKEEDMGQGGRNRLIVWTGISIPYRPA
                     KTDLIETTRRVQRVLADGWVIVIMGEGRIHRGERELLPLADGCAYFALRAGVPVVPIA
                     INGTSWLGFGRRVRVRVGTPIPAEGRATREAVDALTARTTDSLRALVADFPDPPPPGR
                     FGRWLTELFNDWPEGARPELGAGDGAGTERADPGVPPPAS"
     gene            3369..3734
                     /locus_tag="AC162A_06500"
     CDS             3369..3734
                     /locus_tag="AC162A_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010527.1"
                     /translation="MGATGLSTDAKEYRSRLDGQEDAQIDAWVAELMRDVVIRRGIVK
                     VVDDFRRATRLSEPEFERVFASGGGAPAAVGHDAQGRLIVPTITLWALVPGLRAQVPD
                     ARQRLIEYLVANFNELVYV"
     gene            3832..4680
                     /locus_tag="AC162A_06505"
     CDS             3832..4680
                     /locus_tag="AC162A_06505"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HIR5010528.1"
                     /translation="MARLVHPFPSILDGVVVALVALVAGGDTATAVRLGLSMMAFQFA
                     IGALNDLVDAPVDRIGKPDKPIPAGLVTPSAVRGIVVGSALIGLVLAAPGGLALLVIG
                     VVGLSIGAWYDLRAKGTTLSWLPFAIGIPLLPVFGWYGAVGSLPGVFLVLVPAAANAG
                     TALAIANAVVDMERDEAAGIDSIALSLGARRSGWLIVGLQGVVAALALGTAAVVGAPA
                     GWAAAVLITACVPLAGGALGLVAIYQGRGPGWRELAWEIQAVGVGLLAVAWLGALSAA
                     GGMAPA"
     gene            complement(4701..5714)
                     /locus_tag="AC162A_06510"
     CDS             complement(4701..5714)
                     /locus_tag="AC162A_06510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR5010529.1"
                     /translation="MARTDFRAAAARRLSEERELPPAIVSLLSSETTHRSTGVRHVPV
                     DRIDSNPQQPRLAFDEVTLEELAASIREHGVLQPILVRPRDDGRYQLIAGERRWRASR
                     IAEQATIPALIEDIDDDTALEISIIENLQREDISPLDEAAMYDRMIREHGYSIRRLAD
                     KLGKDKGYLENRLRLADAPPEVRALVSLRKDTLSHAYELMKVSDPKKRRRLADQVARN
                     ELSLVKLREKIDGRPRREAVAADDDTDVAIESEAPDKTEAAEGWDERARVAIPIADDS
                     LVSAKQQLNDAVEELLGVIRSQDVLDAIGPTDRANLAKYLTIAKLRLENAIAVIRSGD
                     TDR"
BASE COUNT          786 a         2062 c         2110 g          871 t
ORIGIN      
        1 gaacaggaac tgcatgggac ctccgtgtgg agcccactgt acccagcgaa cgtgacagcc
       61 cggtgacgcc gtcaggtcgt ccgctgggcg ggtcaccgga tgcatgctgc gtcgatcggt
      121 tcgttccacg gcacgcctgt cgcgtgggcg gccaccgccc gcgccaatcg tgccgcccat
      181 cgggtcaggc ggcgcatggt gcgaccggtc gtccgatgag cggccagcgc tcaggtggtc
      241 gggcggttcc tgcgcatcgc gcccacgatc agcgccgctc cgatcacgat cacgagcacg
      301 ggccacacga gttgccagtc gatgtggatg tactgggcga cgaggaacca gacgccgagc
      361 ccgacgagga tgaacccgaa cacgattccg gcgttggtag gatcgggcgc cttccacgac
      421 gggaccgatc cctgcggcgg cgtccacgat ccgctccccg aaggcggcgg cgtccacgat
      481 ccgccaccgg gcggcggcgt ccactggcca ccggacggag gcggcgtcca ggtcccgccc
      541 gcctgcgggg gcggcccgac gctcgggacc cagcccgccg ggggcagcgg gacgacgatc
      601 atcatcacga tgtaaacgag caggaagatc ccgcccgaaa acaccgcgag cacgacccag
      661 gcgacgcgaa cgatcgacgg atcgacccgc agccatgtcg cgaggccacc cgccacgccc
      721 gcgatgacgc ggtcggtcgg ggatcggtac agacggtcag tcacagccgc cctccgggtt
      781 cagcgtgacg ttgccgaggt tcgcgctcac gctcagggcg atctcctgct ggctcgtctc
      841 gagccccggg cggacccagg tatcgccgac gtgggccagc ccccgactct cgaagttgtt
      901 cgatcccagg ctggtggatg aggtgatccg caggccgacg ccatcaggca cgcacaactc
      961 gaccgacccg gcattggctg agatgctccc gttcaccgtg ccggtcggcg tgggcagcgt
     1021 gagcgacagc gagccggcgt tcgcgctcac gtcgagggtc tggagccccg tgacgtcgcc
     1081 catgtcgatc gtcgcgctgc ccgcgttcac cgacgccgag aggttcggca cctgcgtcga
     1141 ccccagatct gcgcgcaccg tgccggcgtt cgccgacacg ccgaggtgga tcgagacgtc
     1201 cgccggcagc gtgacctgcc agctcgaggc gtgaccgagg tcgatcccgg accggtgcgg
     1261 cgaggccacc ttgaggtcct ggccggacgt gatcgtgggc gggatgccgc cgtcggaggt
     1321 gccgctcacg ctccagccgc tgcccgccgc cgtcgcgatg acggcatcgc cacagctgat
     1381 gtcgaggtcc acgctggccg tggtcgcgag cgtgccggac tggctgggga acgggtcgcc
     1441 ctgggcatca ccgccgagcc cacaggttcc gaaccccgcc ccgggggaga agccgaccgc
     1501 gaaccacccg ccagccagca ggccgcaggt gacggcgacg atcaggccgc cgacgaccgc
     1561 cgcccgcgtc cgctgaagga tcagcccgag gccgatgccg atcaggatca gcggccacag
     1621 ctgccagacg cgccggatca cctccgggtc cacggccccc gcctggacga ggagcggaac
     1681 ggacccggcg acgatgagga agacgcccca gccgagcatt cctcgattga tgtgcacgga
     1741 ttcacctccg ggccgatttg gccggccgag gacatgatcg ccggaccgcg caaggttcgc
     1801 gagggccaaa tgtcgcaatc cgggcgcgct agccttgccg ggtgagcgag ccccgccgat
     1861 tcgacgccgt cctgttcgac ctcgacgggg tcctcgtcga cagcgagccg tggtggaacg
     1921 acgtgcggat cgccttcgcc ggcgcccacg gtcgacgctg gaccgacgac gatcaccacg
     1981 cggtgatggg cgcgaactcc cgcggctggg cgatcaccat gcgcgatcgg ctggagctcg
     2041 atgggatgaa cctggacacg attcaggacg cggtggtcgc gggcgtggtc gagcgctatc
     2101 ggaccgagcc accgccggtg atccccggcg ccaccgaagc cgtgcgccgg atcgccgccc
     2161 aggtgcccgt cgcgatcgcg tcatcggccc accccgacgt catcgccgcc gcgatcgacg
     2221 cgctcgggct gcgcgacgtc ctgggcgcga tcgtgtcctc ggacgacgtg ccgcacggca
     2281 agccgtcccc ggatgtctac cttgccgccg cctcgcagct gggggtcgag cccgcccgct
     2341 gcctcgtggt cgaggactcc gtcaacggcg tcaaggcggg caaggccgcc gggatgttcg
     2401 tggtcctggt gcccaacgag agcgtgccgc cgcgccccgg gaccgcggac ctggccgatc
     2461 tcgtcatcga gcgcctcgcc gacctcgacc cagggcgcgt cgcggcctga tcgggcgccg
     2521 cccgagtccg tagactgcgg cggtggccac gcccctcacg gccggtcccg tctaccggcc
     2581 gatccgcgtc cggatcgcgc ggctcatctc cgccatcctc gtgcggagcg tggtccggct
     2641 gcggttcgag ggaagagatc gcctgcccgg cggacccgcg atctacgtgt cgaaccacct
     2701 caactgggct gacccagtgg tcctcctcgc cgtgctgccc gggcgtccga aggtcgcgct
     2761 gttcggcccc aaggaagagg acatgggcca gggaggacgc aaccggctca tcgtgtggac
     2821 gggcatctcc atcccgtatc ggccggccaa gaccgacctg atcgaaacga cccgccgggt
     2881 ccagcgcgtg ctcgcggacg gctgggtgat cgtgatcatg ggggagggga ggatccaccg
     2941 cggcgagcgc gagctgctgc cgctcgccga cggctgcgcc tacttcgcgc tgcgggccgg
     3001 cgtcccggtg gtcccgatcg cgatcaacgg cacgagctgg ctcggcttcg ggcggcgggt
     3061 ccgggtgcgc gtcggcacgc cgatccccgc cgagggccgc gcgacccgcg aggcggtcga
     3121 cgccctcacc gcacgcacga cagattcgct gcgtgcgttg gtcgcggact tccccgatcc
     3181 gccgccgccc gggcgcttcg ggcgctggct gaccgagctg ttcaacgact ggcccgaggg
     3241 cgcacggccg gagctcggcg ccggagatgg tgccgggacc gaacgcgcgg atcccggggt
     3301 cccgccgccc gcgagctgag gcccgcgtat ggcatcctgc tggcgacgac cgaacgccag
     3361 gaggattcgt gggcgccacc ggcctctcca ccgacgcgaa ggaataccgc tcgagactcg
     3421 acgggcagga agacgcccag atcgacgcgt gggtcgccga gctgatgcgt gacgtcgtga
     3481 tccggcgtgg gatcgtcaag gtcgtggacg acttccgccg cgcgacccgc ctgagcgaac
     3541 ctgaattcga acgggtgttc gcttccggag gcggggcacc ggcggcggtc ggccacgatg
     3601 cgcagggccg gctgatcgtg cccaccatca cgctctgggc gctcgtgccg gggctgcggg
     3661 cccaggtccc cgacgcccga cagcgcctca tcgagtacct cgtcgcgaac ttcaacgagc
     3721 tcgtctacgt ctgacgacgg acgcgccgtc gacccgctca cggcgttcga cgaacctccc
     3781 gaggacgagc cgcggcggcc gcggcgctca tggtgggcga ctgccgtcgg tatggcgcgg
     3841 ctggtccacc cgttcccgtc gatcctcgac ggggtcgtgg tcgctctcgt ggccctcgtg
     3901 gccggcggcg ataccgcgac ggccgtccgt ctcgggctgt cgatgatggc gttccagttc
     3961 gcgatcgggg ccctcaacga tctggtcgat gcgccggtag accggatcgg caagcctgac
     4021 aaaccgatcc cggcgggtct ggtcacgccg tcggccgtgc gggggatcgt cgtgggatcc
     4081 gcgttgatcg ggctcgtgct ggccgcgccc ggcgggctcg cgctgctggt gatcggggtc
     4141 gtcgggctct cgatcggggc ctggtacgac ctgcgggcga agggcacgac gctctcgtgg
     4201 ctcccgttcg cgatcgggat cccgctcttg cccgtgttcg gctggtacgg ggcggttggc
     4261 tcgctgccgg gcgtgttcct ggtcctcgtg cccgcggcgg cgaacgccgg cacggccctg
     4321 gccatcgcca acgcggtggt cgacatggag cgggacgagg ccgcggggat cgactcgatc
     4381 gcgctctcac tcggcgctcg acgctccggc tggctgatcg tcgggctgca gggcgtcgtg
     4441 gccgccctcg ccctgggcac ggcggccgtc gtcggcgccc ctgctggctg ggccgcggcc
     4501 gtcctgatca ccgcctgcgt gccgctcgct ggaggcgcgc tcgggctcgt ggcgatctac
     4561 caaggcaggg ggccgggctg gcgggagctc gcctgggaaa tccaggccgt cggcgtcggc
     4621 ctgctcgcgg tcgcctggct gggcgcgctg agcgccgcgg gcgggatggc gcccgcctga
     4681 cctcgtcgcg ctggtgcctg ctagcggtcg gtgtcgccgc tgcggatgac cgcgatggcg
     4741 ttctcgagcc ggagcttggc gatcgtcagg tacttggcga ggttcgcccg gtcggtcggc
     4801 ccgatcgcgt cgaggacgtc ctggctccgg atgacgccca gcagctcctc gaccgcgtcg
     4861 ttcagctgct gcttggcgga gacgaggctg tcgtcggcga tggggatcgc gacgcgggct
     4921 cgctcgtccc agccctcagc cgcctcggtc ttgtcaggcg cctcggattc gatcgccacg
     4981 tccgtgtcgt cgtcagcggc caccgcctcc cgccgcggcc gtccgtcgat cttctcgcgc
     5041 agcttgacca gcgacagctc gttgcgggcc acctggtccg cgagccgccg gcgcttcttc
     5101 gggtcactga ccttcatcag ctcgtacgcg tgggacagcg tgtccttgcg caaagacacg
     5161 agcgcgcgca cttcgggcgg ggcgtcggcc aggcgcaggc ggttctcgag atagcccttg
     5221 tccttgccca gcttgtcggc aagccggcgg atgctgtacc cgtgctcgcg gatcatgcgg
     5281 tcgtacatcg ccgcctcgtc gagcggcgag atgtcttcgc gctggaggtt ctcgatgatc
     5341 gagatctcga gcgcggtgtc gtcgtcgatg tcctcgatga gcgcggggat cgtcgcctgc
     5401 tccgcgattc gcgacgcgcg ccatcgacgc tcgccggcga tgagctggta gcgcccgtcg
     5461 tcgcgcggtc ggacaaggat cggctggagc acgccgtgct cgcggatgga cgcggcgagc
     5521 tcttcgaggg tcacctcgtc gaaggcgagc cgcggctgct gggggttgga gtcgatgcga
     5581 tcgaccggaa cgtggcgcac gccggtggag cggtgcgtgg tctccgacga gaggaggctg
     5641 acgatggcgg gcgggagctc gcgctcctcg gacaggcgcc gcgctgcggc ggcgcggaag
     5701 tcagtcctgg ccaagatcga tgacctcccg ggcaaactcg cggtaggttc gggcggcctc
     5761 ggaggtcccc gcataggacg tgatcggccg cccgacgagc ggcgcctccc ccagcttcac
     5821 ggtgttgcg
//
LOCUS       DBNHXO010000308         1934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_484201, whole genome shotgun sequence.
ACCESSION   DBNHXO010000308 DBNHXO010000000
VERSION     DBNHXO010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_484201"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="AC162A_06515"
     CDS             <1..667
                     /locus_tag="AC162A_06515"
                     /inference="COORDINATES: protein motif:HMM:NF017984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA glycosylase AlkZ-like family protein"
                     /protein_id="HIR5010530.1"
                     /translation="GGPWSFGRRPLMVAPEAWLGQRFGSAQDGLELLVRRYLGALGPA
                     TVADLASWVKLARATLRPPVAALDRAGELWHGRDGLGRELIDLVDAPRPGADMDAPPR
                     LLPMWDGLLLGHADRSRVIDPEDRSRLVAANGDTYPTFLVDGRVAGLWWTRRASGDTE
                     IELEPFRPLRPADRAALEAEAAALARFLGPREPEAYATYRARRDRKRSMTALGSPATG
                     TSD"
     gene            816..1292
                     /locus_tag="AC162A_06520"
     CDS             816..1292
                     /locus_tag="AC162A_06520"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR5010531.1"
                     /translation="MSAIPEPTGWEDSLTGLEGPDFWQRILVAEVARAVRYKRNLTVV
                     VAEVEGVETMAETWGWNVGRHAIREAAQCLRRTSRTSDYCTRIGLARFGIILTETDEI
                     AAINFVERVREAGPRTMPRAGDGLRFNFGWASPKSGESADALVRRAEKRLMAEASA"
     gene            1360..>1934
                     /locus_tag="AC162A_06525"
     CDS             1360..>1934
                     /locus_tag="AC162A_06525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5010532.1"
                     /translation="MAASSPAGPAPPELSVIVPAWNAADSIERALGTVLADPDADVEC
                     VVVDDASTDATVSVVERMAASDPRIVLIRSPRNGGASAARNLALDQIRGTWVTFLDAD
                     DILTDVGVEALLGPTRMTDPLAVIGQRVWSDGTKRWVSSLYDIPDIREPGRKSMVSAP
                     GLVYYASATGKAFHRSLVGDLRFEGRVLGDQP"
BASE COUNT          257 a          722 c          684 g          271 t
ORIGIN      
        1 cggtggtccg tggtcgttcg gccgccggcc cctgatggtc gcgccggaag cgtggctcgg
       61 gcagcggttc gggtcggctc aggacggcct cgagctgctc gtgcggcgct atctcggtgc
      121 cctcggacct gcgacggtgg ccgacctcgc gtcgtgggtg aagctcgccc gggccacgct
      181 gcggccgccg gtcgccgcgc tcgaccgggc cggcgagcta tggcacggac gcgacgggct
      241 gggccgtgag ctcatcgacc tcgttgacgc gccgcgaccc ggcgcggaca tggacgcgcc
      301 gccgcgcctg ttgccgatgt gggacgggct gctgctcggc cacgccgacc gatcgcgtgt
      361 catcgacccg gaggaccggt cgcggctcgt ggcggccaac ggggacacct accccacgtt
      421 cctcgtcgac ggacgcgtcg cgggcctgtg gtggacgcgg cgagcctccg gcgacacgga
      481 gatcgagctc gagccgttcc ggccgctgcg cccggccgac cgcgcggccc tcgaggccga
      541 ggccgccgcg ctggcgcgct tcctcggccc ccgcgagccc gaggcctacg ccacgtaccg
      601 cgcgcgccgc gatcggaagc gctccatgac ggcgctcggc tctccggcga ccgggacctc
      661 tgactgacgg taccggtacg accttcgggt ggcgcgtcca cgcaccacgc acgagcattc
      721 cgggtgacct gcaagcaggc catggacgtg agacgttccg cttggaaccg ccgccggacc
      781 gaaggcggcg ctaggagtgc gcggtacctg tgaacatgag cgcgattccc gaaccgacgg
      841 gttgggaaga ctccctcacc gggctggagg gcccggactt ctggcagcgc atcctcgttg
      901 ccgaggtggc ccgcgccgtc cgctacaagc ggaacctcac cgtggtcgtc gcggaggtcg
      961 aaggcgtcga gaccatggcc gagacctggg gctggaacgt cgggcggcac gccattcgcg
     1021 aggcggccca gtgcctccga cgcacgtcgc gcacgtccga ctactgcacg cggatcgggc
     1081 tcgcccggtt cggcatcatc ctcaccgaga ccgacgagat cgccgcgatc aacttcgtcg
     1141 agcgggtccg cgaggcgggt ccccggacga tgccccgcgc cggcgacggc ctgcgcttca
     1201 acttcggctg ggccagcccc aagtccggcg agtcggccga cgccctggtc cgccgcgccg
     1261 agaagcgcct gatggccgag gccagcgcct agccctcatc ggggttcaca acccagggct
     1321 atcctcgacg gacgagagcc acccacgagg agcctgccct tggccgcgag ctcgcctgcc
     1381 ggccccgcgc cgcccgagct gagcgtcatc gtccccgcct ggaacgccgc cgactcgatc
     1441 gagcgcgcgc tcgggacggt gctcgcggac cccgacgccg acgtcgagtg cgtcgtggtc
     1501 gacgatgcct ccaccgatgc gaccgtgtcg gtcgtcgagc gcatggccgc gtccgatccg
     1561 cggatcgtgc tgatccgctc gccacggaac ggcggcgcct cggccgcccg caacctcgcc
     1621 ctcgatcaga tccggggcac ctgggtcacg ttcctcgatg ccgacgacat cctgaccgac
     1681 gtcggggtcg aggccctgct cggtccaacc cggatgaccg atcccctcgc cgtcatcggc
     1741 cagcgagtct ggtcggacgg caccaagagg tgggtcagct ctctgtatga catccccgac
     1801 atccgcgagc cgggccgcaa gtccatggtc tcggcgcccg ggctcgtgta ctacgcgtcg
     1861 gcgaccggca aggcgttcca ccggtcgctg gtcggcgacc tgcgcttcga ggggcgcgtg
     1921 ctgggcgacc agcc
//
LOCUS       DBNHXO010000309         1620 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3460264, whole genome shotgun sequence.
ACCESSION   DBNHXO010000309 DBNHXO010000000
VERSION     DBNHXO010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3460264"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1620)
                     /locus_tag="AC162A_06530"
     CDS             complement(<1..>1620)
                     /locus_tag="AC162A_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR5010533.1"
                     /translation="GVHPTARYTSHELTADDDACIPGFARLAEVAHANRSVIFGQLFH
                     GGREIMDTEEGTLAVALAPSAVPTERFHVMPRAMPVPLIREIVAGFGTSAARLERAGL
                     DGCEVVASHGYLPAQFINPRTNLRTDDYGGDETRRLRFLRDALAAARAATRPGFVVGL
                     RISIGETSPEGMTEDDALTAIAALDAVGAMDYVSVVAGTSATLAGSDHIVPPTPWPNG
                     YTAPLAARAKQAVRVPVFVAGRISQPQEAEAILAAGQADACVMTRALICDPELPAKTA
                     AGRLDDIRACIGCNQACIGHFHAGVPISCIQFPESGREREFPRVGRPGEERHPRAAVT
                     RDVLVIGGGPGGLKAAAVAAERGHRVTLVEAGRRVGGQVLLAQELPERAEIGGAINNL
                     LGEAQRSGVDIVTGVRAHAAWVRERGPDVVIVASGAVPYRPPLEVMGAPVILQTWDVI
                     RGAAVPDGHVVVADFRSDWTGLGVATMLASRGHRLTLAVVGTMAGQPVQQYIRDTMTA
                     TARRAHVDIRPTLRLYGADSNAVFLQDVLTG"
BASE COUNT          207 a          595 c          611 g          207 t
ORIGIN      
        1 tcaccggtca ggacgtcctg gaggaagacg gcgttcgagt cggcgccgta caggcgcagc
       61 gtcggccgga tgtcgacgtg ggcccggcgc gcggtcgcgg tcatcgtgtc gcggatgtac
      121 tgctggaccg gctggcccgc catcgttccg acgaccgcga gcgtcagccg gtggccgcgc
      181 gaggcgagca tcgtcgcgac cccgagcccg gtccagtccg accggaagtc ggcgacgacc
      241 acgtggccgt cggggacggc cgcgccgcgg atgacatccc aggtctggag gatcaccggc
      301 gcgcccatga cctcgagcgg cggccgatag gggaccgcgc cggacgcgac gatcacgacg
      361 tccgggccac gctcgcggac ccacgcggcg tgggcgcgga cccccgtcac gatgtccacc
      421 cccgatcgct gcgcctcgcc cagcaggttg ttgatcgccc ccccgatctc agcccgctcg
      481 ggcagctcct gggccagcag tacctggccg ccgacccgcc gcccggcctc gactagcgtg
      541 acccggtgac cgcgctccgc cgcgaccgcc gcggccttga gcccgcccgg cccgccgccg
      601 atgaccagca cgtcgcgagt gacggccgcc cgtgggtgcc gctcctcgcc gggcctgccg
      661 acgcggggga actcgcgctc gcggccgctc tcggggaact ggatgcacga gatggggacg
      721 ccggcgtgga agtggccgat gcacgcctgg ttgcagccga tgcacgcacg gatgtcgtcg
      781 agccttcccg cggcggtctt ggccggcagc tccgggtcgc agatgagcgc ccgggtcatg
      841 acgcaggcgt ccgcctggcc ggcggcgagg atcgcctccg cctcctgcgg ctggctgatc
      901 cggccggcga cgaacactgg cacgcgcacg gcctgcttgg cccgagccgc gagcggcgcg
      961 gtgtagccgt tcggccacgg cgtcggcggc acgatgtgat cggagccggc gagggtcgcg
     1021 gacgtccccg cgacgacgct cacgtagtcc atcgccccga ccgcgtcgag ggccgcgatc
     1081 gccgtcagcg cgtcgtcctc ggtcatcccc tcgggcgatg tctcgccgat cgagatccgc
     1141 agcccgacca cgaaccccgg acgggtggcg gcccgcgccg cggcgagcgc gtcgcgcagg
     1201 aacctgagcc ggcgtgtctc gtccccgccg tagtcgtcgg tccgcaggtt cgtacgcggg
     1261 ttgatgaact gcgccgggag gtagccgtgc gaggcgacga cctcgcagcc atcgagcccg
     1321 gcccgctcga ggcgcgccgc ggacgtgccg aacccggcga cgatctcgcg gatcagcggc
     1381 accggcatcg cgcgcggcat gacgtggaag cgctcggtcg ggaccgcgct gggcgcgagg
     1441 gcgacggcga gcgtgccctc ctcggtgtcc atgatctcgc ggccgccgtg gaacagctgg
     1501 ccgaagatga ccgagcggtt ggcgtgggcg acctcggcaa ggcgtgcgaa ccccgggatg
     1561 caggcgtcgt cgtcggcggt cagctcgtgg gaggtgtagc gggcggtcgg gtggacgccc
//
LOCUS       DBNHXO010000310         2532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_88122, whole genome shotgun sequence.
ACCESSION   DBNHXO010000310 DBNHXO010000000
VERSION     DBNHXO010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_88122"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="AC162A_06535"
     CDS             <1..471
                     /locus_tag="AC162A_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR5010534.1"
                     /translation="RVLVAGEPVTQEPPGPSPIVARSDLVGVGRDDVDPGTPEASLLA
                     LLRPGATMLLTDGAAGGIALEAGPGGSVARRRARSPIPVDRFVDPVGAGDTFLAGVFA
                     ARVDPSLVGPEDGPDADLLVGAAAASLICEGPGLLGVPARAAVLRRMHRASRPG"
     gene            complement(468..1961)
                     /locus_tag="AC162A_06540"
     CDS             complement(468..1961)
                     /locus_tag="AC162A_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HIR5010535.1"
                     /translation="MTIEPAGPVTRERRLANLRATLARPGGPPLGPEQPPARAPFAGR
                     RSPILATRLASALGADVAEIAGGWLVRRTVAPIELPLDRERLADLPGHPPPDAPLVCI
                     DTETTGLATAAGTLAFLVGLARWEGDAFCQTQLLLPDHADEPALLAEIAAWVTPDAWL
                     VSYNGRGFDWPLIEARFRLAGRPAPAHAGHLDLLPFVRRVFRHRMEDARLRTVEGELL
                     GVSRIGDVEGWEIPGIYLDVLRGGPVDALAGVVIHNEKDVRSLGLLLAHVERRYADRA
                     ARHDAPRGDLAGLARAYGRDKRNEEALTCLDDALASAPPIRDPFGRTPVAQALARERL
                     EDERDAWWEARSQPTFGGRPPRSGWRGPLANGRGGPLGPAATAAAWDTGGTWDAASRW
                     TDERLAAERARLLRRLGRWTEAAEAWQTAAAAGDGLGVIAWIEVAKLREHRLADPGGA
                     LAATRAAWRLLERLRRLGRAHPRLEADLERRGARLTARLRRAEASSP"
     gene            complement(1958..>2532)
                     /locus_tag="AC162A_06545"
     CDS             complement(1958..>2532)
                     /locus_tag="AC162A_06545"
                     /inference="COORDINATES: protein motif:HMM:NF020926.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HIR5010536.1"
                     /translation="LDVFASTPATGGSRVHGEVMVASLVTLYKKLKFITDENVGWGPI
                     DLPEIELQTTAYWLTAEDAGAGWRRDELDVALIGAGRAIQAVAAVLLMVDPRDLGLVA
                     QVRSPHHEAPTIYLYEAMPGGVGLAERLFDRHDELVTGATGLILACACDAGCPACTGP
                     RLEPGVDGKALDLRLLAALTGDVREPVAAR"
BASE COUNT          291 a          969 c          964 g          308 t
ORIGIN      
        1 cgggtgctcg tcgccggcga gcccgtgacc caggagccgc ccggcccgtc gcccatcgtc
       61 gcacgctcgg acctggtcgg cgtcgggcgc gacgacgtcg atcccgggac acccgaggcg
      121 tcgctgctcg ccctgctccg tcccggcgcg acgatgttgc tgacggacgg cgccgccggc
      181 ggcatcgccc tcgaggcggg acccggcggg agcgtcgcgc gccgccgcgc caggtcgccg
      241 attccggtcg atcggttcgt ggacccggtt ggcgccggcg acacgttcct cgccggggtg
      301 ttcgccgcgc gcgtcgatcc gtcgctggtc ggccccgagg atggccccga cgcagacctg
      361 ctcgtcgggg ccgccgccgc ctccctgatc tgcgagggcc cggggctgct cggcgtcccg
      421 gcgcgggccg ccgtcctccg ccggatgcac cgggcgtcca gaccgggcta gggcgaggac
      481 gcctcggcgc gccggagccg tgcagtcagg cgcgccccgc gccgctcgag gtcagcctcg
      541 aggcgggggt gggctcgccc gaggcgcctg aggcgttcca gcagtcgcca cgcagctcgg
      601 gtcgccgcca gggcgccgcc ggggtccgcc agccgatgct cgcgcagctt ggcaacctcg
      661 atccacgcga tcaccccgag cccatcgccc gcggccgcgg ccgtctgcca ggcctccgcg
      721 gcctcggtcc atcgcccgag ccgccgcaac aggcgtgcgc gctcggccgc gagccgctcg
      781 tcggtccagc ggctggcggc gtcccaggtc ccgccggtgt cccaggccgc cgccgtcgcc
      841 gccggcccga gcgggccgcc gcggccgttc gccagcgggc cccgccagcc ggagcgtggc
      901 gggcgcccgc cgaacgtcgg ctgggatcgc gcttcccacc atgcgtcccg ctcgtcttcg
      961 agccgctccc gggccagcgc ctgcgcgaca ggcgtgcgtc cgaacgggtc gcggatcggc
     1021 ggtgccgacg cgagggcgtc atcgaggcag gtgagggcct cctcgttgcg cttgtcgcgg
     1081 ccgtaggcac gggcgagccc ggcgagatcg cctcgcggcg cgtcgtggcg ggccgcgcgg
     1141 tcggcgtatc ggcgctctac gtgcgcgagg agcaggccga ggctgcggac gtccttctcg
     1201 ttgtggatca cgacgcccgc aagcgcgtcc accggaccgc cgcgcaggac gtcgaggtag
     1261 atgccgggaa tctcccagcc ctcgacgtcg ccgatgcggg agacgccgag gagctcgccc
     1321 tcgaccgtcc gcaaccgggc gtcctccatc cgatgccgga agacgcggcg cacgaacggg
     1381 aggaggtcca ggtggccggc gtgtgccggc gccggtcgcc cggcgaggcg gaaccgggcc
     1441 tcgatcagcg gccagtcgaa gccgcggccg ttgtagctca ccagccacgc gtcgggggtg
     1501 acccacgcgg cgatctccgc aagaagggcc ggctcgtcgg cgtggtcggg cagcagcagc
     1561 tgggtctggc agaaggcgtc accctcccag cgcgcgagcc cgacgaggaa cgcgagcgtg
     1621 cccgccgccg tcgcgagccc cgtggtctcg gtgtcgatgc acacgagcgg cgcgtcgggc
     1681 ggtgggtggc ccgggaggtc ggcgagccgc tcgcggtcga gtggcagctc gatgggcgcg
     1741 accgtccgtc gcaccagcca ccccccggcg atctcggcga cgtccgcgcc gagcgccgag
     1801 gcgagccgcg tcgcgaggat gggcgagcgt cgccccgcga acggggcgcg cgccggcggc
     1861 tgttccggac cgaggggcgg accgccgggt cgcgccaggg tcgcccgcag gttcgccagc
     1921 cggcgctcgc gggtgacggg gcccgcgggc tcgatcgtca tcgcgccgcc acgggctcgc
     1981 ggacgtcgcc agtgagcgcc gcgaggagcc gcaagtcgag cgccttgccg tcgacgccag
     2041 gctccaggcg cggaccggtg caggccggac agccggcgtc gcaggcgcag gcgaggatca
     2101 gtcccgtcgc cccggtcacc agctcgtcgt ggcggtcgaa caggcgctcc gcgaggccga
     2161 cgccgcccgg catcgcttcg tacaggtaga tcgtgggcgc ctcgtggtgc ggcgaccgga
     2221 cctgcgccac gaggccgaga tcccgcggat cgaccatcag cagcacggcg gccacggcct
     2281 ggattgcccg gcccgccccg atcagcgcca cgtcgagctc gtcgcgccgc cagccggctc
     2341 ccgcgtcctc ggcggtcagc cagtacgcgg tcgtctggag ctcgatctcg ggcagatcga
     2401 tcggtcccca gccgacgttc tcgtcggtga tgaacttgag cttcttgtac agcgtcacga
     2461 ggctcgcgac catgacctcg ccgtggaccc gcgacccgcc ggtcgccggc gtcgaggcga
     2521 acacgtcgag cg
//
LOCUS       DBNHXO010000311         1913 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_88273, whole genome shotgun sequence.
ACCESSION   DBNHXO010000311 DBNHXO010000000
VERSION     DBNHXO010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_88273"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1913)
                     /locus_tag="AC162A_06550"
     CDS             complement(<1..>1913)
                     /locus_tag="AC162A_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010537.1"
                     /translation="RLARAAAEMEAPPTRGLPPRSTSLPRRRTGGLDADLAAGLAEAL
                     EHGFVRADGDERLSIRHELVARAVVADLLPTQRTRYRAALAVAFDDIPVVAAAHWRAA
                     HRLGEAREAALAAGRLALRVEAPQDALEALELGLDLLVPEDGEPESRDAVAGNPGAPS
                     TTDEESALAIGRLAAEAAYAAMRPTRAVAYAESTLASLNERRDPMAYAVMQGRLGRYR
                     VAAGDAAGATHALRKAATVVPPEPSLERARILALLAQERMIAGAFSDSDRAATEALEI
                     ARDQGDDAEPEAIHAMTTLAVVRAWGDDPEEGVRLLRQSLERARELGLVEEWWRAGAN
                     MAVVLELLGRPGEAIDEAFRGMAEARAMDLEAVYANLLGGNVAGILVDVGRWPEARDL
                     SLRALDWSPAGIPFVNAILNLVIVEIESEAGEEAGRLLGRVLVELETGGDFQFAVPAY
                     QATASYAMWSGDLPDARRAAERGWARVRGTEDWVLMARMASTALEVESAIVAEALEKR
                     RIGDVAASRERAGRILAEADSAVTRARPDDDGAGRGEPEASIATARAFHARLQGRDDP
                     AAWADIAARWHDLGDPYREARARWHQAEAILGGATAGASGTRARTDARVVRADAREPL
                     ASAVELGLRLRARP"
BASE COUNT          221 a          769 c          692 g          231 t
ORIGIN      
        1 ggtcgcgccc gcaggcgcag cccgagctcc accgccgacg ccaggggctc gcgggcatcg
       61 gcgcgcacga cgcgggcatc cgtcctcgcc cgtgttccgg acgcgcccgc cgtggcaccc
      121 ccgaggatcg cctccgcctg gtgccaccgg gcccgcgcct cccggtaggg atcgcccagg
      181 tcgtgccagc gggcggcgat gtcggcccac gcggcggggt cgtcgcggcc ctgcaaccgc
      241 gcgtggaagg cgcgggcggt cgcgatgctc gcctccggtt cgccgcgtcc cgcgccgtcg
      301 tcatcgggcc gcgcccgcgt caccgcgctg tcggcctccg ccaggatccg gccggcgcgc
      361 tcgcgtgacg ccgcgacgtc gccgatccgg cgcttctcga gggcctccgc gacgatcgcc
      421 gactccacct cgagcgctgt cgaggccatg cgtgccatca gcacccagtc ctcggtgccg
      481 cggacacggg cccagccgcg ctcggccgcg cgacgggcat ccggaaggtc gccgctccac
      541 atcgcgtagg aggccgtcgc ctggtaggcg ggcaccgcga actggaagtc gccgccagtc
      601 tcgagctcga ccagcacgcg accgagcagc cggcccgcct cctcgcccgc ctcggactcg
      661 atctccacga tcacgaggtt gaggatggcg ttcacgaacg ggatccccgc cgggctccag
      721 tcgagcgccc gcaggctcag gtcccgggcc tccggccagc gaccgacgtc gacgaggatg
      781 ccggccacgt tgccgccgag gaggttggcg tagaccgcct ccaggtccat cgcacgggcc
      841 tcggccatgc cccggaacgc ctcgtcgatc gcctcgcccg gtcggcccag gagctcgagc
      901 acgacggcca tgttcgcgcc ggccctccac cactcctcga cgagcccgag ctcgcgagcg
      961 cgttcgagcg actggcgcag caaccggacg ccctcctccg gatcgtcgcc ccacgcccgc
     1021 accaccgcga gcgtcgtcat cgcatggatc gcctcgggct ccgcgtcgtc gccctggtcg
     1081 cgagcgatct cgagcgcctc cgtggccgcg cggtcagagt cgctgaaggc accggcgatc
     1141 atccgttcct gcgcgagcag ggcgaggatc ctcgcgcgct cgagcgacgg ctcgggcggg
     1201 acgacggtcg cggccttccg cagggcgtgc gtcgcgccag ccgcgtcgcc cgcggcgacc
     1261 cggtagcggc cgaggcgacc ctgcatcacg gcgtaggcca tcgggtcacg ccgctcgttg
     1321 agcgacgcga gcgtgctctc ggcgtaggcc acggcgcgcg tcggtcgcat cgcggcgtac
     1381 gccgcctcgg cggccagccg gccgatcgcg agggccgatt cttcgtcggt cgtcgatggc
     1441 gcaccggggt tgccggccac ggcgtcgcgc gactcgggct cgccatcctc cggcaccaac
     1501 aggtccagcc ccagctcgag cgcttccagc gcgtcctgcg gcgcttcgac gcgcagcgcg
     1561 agccggccgg ccgcgagggc cgcctcgcgc gcctcgccca gccggtgcgc cgcccgccag
     1621 tgcgccgccg cgacgacagg gatgtcgtcg aaggccaccg cgagggccgc gcggtaccgc
     1681 gtccgctgcg tgggcagcag gtcggcgacg acggcgcggg cgacgagctc gtgccggatc
     1741 gagaggcgct cgtcgccgtc cgcgcgcacg aagccgtgct cgagggcctc ggcaagcccg
     1801 gcggcgaggt cggcgtcgag ccctccggtg cgccgtcgcg gcagcgacgt cgatcgcggc
     1861 ggcaggccgc gcgtcggcgg cgcctccatc tccgcggccg cgcgggccag ccg
//
LOCUS       DBNHXO010000312         6040 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_288552, whole genome shotgun sequence.
ACCESSION   DBNHXO010000312 DBNHXO010000000
VERSION     DBNHXO010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_288552"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="AC162A_06555"
     CDS             complement(<1..710)
                     /locus_tag="AC162A_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR5010538.1"
                     /translation="MGELRLGLNLWSQASDWPSFLDAARRAERLGFDHLWTWDHILAI
                     FGDPHQPIFEGYTALAAAAQATERIRLGLFVGANTFRNPGLAVKALTTIDHISGGRAI
                     MGMGGAWFGEEHRAFGIDFGSGFGERLDWLAEAAEATRTLLDGGEVTSAPDGRYAFDH
                     LRIQPLPIQERLPIMIGGAGEKKTLRIVARHADMWNAFGTPETLAAKDAILRAHCADV
                     GRDPEAIERSVGCKITIRS"
     gene            916..2340
                     /locus_tag="AC162A_06560"
     CDS             916..2340
                     /locus_tag="AC162A_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5010539.1"
                     /translation="MRILRRVPVLAVLAIVAVACSSGGTTTAAPGGSTAASTPAPATA
                     PATAEASAPASESAPPAAVATPEPAKDCARTSDESAMQMWERSGGNKGMVDQLVCDWN
                     TANPGKLINLSYIPHAEMVGKIAQATASGEVPDLMGMDLIYAPQFENAGQLVDLTDKI
                     SSWPELTTASPGHMTVATFNDRLYGVPLYADVSALFYNKDLFKQAGLDPNKPPTSLAE
                     IREYADKISALGGDIKGYYLPGNCAGCNIFTVGPLMWATPETTIEAATCESEPLVGPG
                     VPEVLQWARDMIKAGNVHDTDRAETGETFHLQFGSGKVGMMGTGNFNITLANDQMKDH
                     PFEFGITNLPGKDPGSSASFIGGDLVVVPQGSERVDDAVEFMKFILSDNEQVEGYAKL
                     LNLTTRSDMANNKYFQEEPLVQDVAKALEVGTTPYTLTFFEQINSPQGPWLQMLQKAY
                     YTDEPIDSIIASAKEQMKQIACQS"
     gene            2478..3356
                     /locus_tag="AC162A_06565"
     CDS             2478..3356
                     /locus_tag="AC162A_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5010540.1"
                     /translation="MLATRRNKLMGLLFLSPALLFVLAFTAYPLSQLVWMSFNSWSLI
                     TPPKFIGTGNFTRAFSDDQFWVSLTYSLKYTLFITPILIIGGYLIALLVADNSRIKRV
                     TRTMVFIPVVIGLGVSSLLWYWLYNPLFGLIDTALLDLGLISKPILWLGTEEGTSTWA
                     IIGSVTWKVIGFGMLLFVAAIQGIPAEVNEAAVVDGASFRQRVWRVTVPLTAPTILLV
                     TLISVIGSLLAFDQFYIMTAGQPFNQTATAVFYVYLNSFPYLKLGYGAALSLLIAGVV
                     LLFTVVQLWFTRRSRQ"
     gene            3353..4252
                     /locus_tag="AC162A_06570"
     CDS             3353..4252
                     /locus_tag="AC162A_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020512736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5010541.1"
                     /translation="MSTETLEAAQPRADTRSLRSRAWSGRNKYLVGALCLAICAVMVA
                     PVVFSILASVKTTAEAAAQPPTYLPGELSLDSYSRLWTYQAGLPAYLANSVGTALLTI
                     VIALGLTIPAGYALARFPIPFKEPLFVFLLLALIIPYQALLTPMFLMFVKLKLTNTIL
                     GLAILHTTIQLPFSLYIMRNSFEAVPRELEEAAYIDGGSPLQVLRRVFLPMVVPAIVT
                     VALFAFITSWNELLGSLVMNSKESAFTLPVILAAARTETSLGGTDWGMLQAGVTISII
                     PCILVYVLLQKYYVSGLLSGAVK"
     gene            4365..>6040
                     /locus_tag="AC162A_06575"
     CDS             4365..>6040
                     /locus_tag="AC162A_06575"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019556.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HIR5010542.1"
                     /translation="MNARVGNPAARVASPIAVVVPSPDAITRVRPLGGQDVGITGGLW
                     HERLRVNREQTLPHVFGQLVEVGNLHNLRLAAGAGGQYQALGIMFDGPFPFLDSDVYK
                     WLEGVAWELGRTWDPAIAAMADEAIDAIAAAQREDGYLNTFVQVLAPGREYKDLQFGH
                     ELYCIGHLIQAGVAWHRALGDDRLLAISRRAADSVDAALGPNGATGIDGHPEIEMALV
                     ELYRATGEARYLELARLHVERRGHGLLGAGRFGAPYWQDHLPVREATDVAGHVVRQLY
                     LDAGAVDVATETGDTDLLDAVHRRWRDMVATRSYLTGGVGSRHEHEGFGDPYELPPDR
                     AYAETCAAIASTMLAWRLLLATGDPDCADVIERTIYNGVLSGVSTDGLRFFYVNPLQR
                     RTHRVAADSGTGERAPWYACACCPPNLVRTFGSWPQYLATTDDDGIQIHQYAAAEIRA
                     TLPGGDVALAVETDYPWDGRVRITVRQTPDTPWSLNLRVPGWVDAGSVDWYAAAEGAV
                     DAHPPSPIAAGDRLVTCSRVWHAGDAVVLDLDMPGRVTRPAPQVDAIRGCV"
BASE COUNT          932 a         2157 c         2009 g          942 t
ORIGIN      
        1 gaccggatcg tgatcttgca gccgaccgag cgctcgatcg cctcgggatc gcgcccgacg
       61 tccgcgcagt gcgcgcgcag gatcgcgtcc ttggccgcca gggtctcggg cgtgccgaac
      121 gcgttccaca tgtccgcgtg acgcgcgacg atccgcaggg tcttcttctc gcccgcgccg
      181 ccgatcatga tcgggaggcg ctcctggatc ggcaggggct ggatccgcag gtggtcgaag
      241 gcgtagcggc catcgggcgc gctcgtgacc tcgcccccgt cgagcagggt ccgggtcgct
      301 tccgcggctt cggccagcca gtcgaggcgc tcgccgaacc cggatccgaa gtcgatgccg
      361 aaggcccggt gctcctcgcc gaaccacgcc ccgcccatgc ccatgatcgc ccggccaccg
      421 ctgatgtggt cgatcgtcgt cagcgccttc acggccaacc cggggttgcg gaaggtgttg
      481 gcaccgacga acagcccgag ccggatccgc tcggtcgcct gggcggccgc cgcgagcgcg
      541 gtgtagccct cgaagatcgg ctgatgcgga tcgccgaaga tcgccaggat gtggtcccag
      601 gtccagagat ggtcgaagcc gagccgttcg gcccggcggg cggcgtccag gaacgagggc
      661 cagtccgacg cctggctcca caggttcagg ccgaggcgga gctcgcccat cggtgcctcc
      721 taggggtgag ggagaagcgt aaccgggctg tccgcgggcc gctcatccgc agttccgatc
      781 gattgactgc aaacgaatgt atgcactcga ttgctcgttc gaaaacgtgg tagtgtgcgc
      841 aacgccgagg gccgcggacc ggaagcatct cgccacccgc tagccgccgc ccggcttggc
      901 aaggaggagt tacggatgag gatccttcgc agggtgcccg tcctcgcggt cctggccatc
      961 gtggccgtcg cctgttcatc cgggggtacg acaacggccg cccccggcgg gtcgaccgcg
     1021 gcgtctaccc cggcacccgc aacggcaccc gcaacggccg aggcatcggc cccggcgtcc
     1081 gagagcgcgc ccccggcggc cgtggccacc ccggagcccg ccaaggactg tgcgcggacg
     1141 agcgacgagt ctgccatgca gatgtgggag cgctcgggcg gcaacaaggg catggtcgac
     1201 cagctcgtgt gcgactggaa cacggccaac ccgggcaagc tcatcaacct gagctacatc
     1261 ccgcacgcgg agatggtggg caagatcgct caggcaaccg cgtcgggcga ggtcccggac
     1321 ctcatgggca tggacctgat ctatgccccg cagttcgaga acgccggcca gctcgtcgac
     1381 ctgactgaca agatctcgag ctggcccgag ctgacgacgg cgagccccgg tcacatgacc
     1441 gtcgccacgt tcaacgaccg tctgtacggc gtcccgctct acgcggacgt ctcggcgctg
     1501 ttctacaaca aggacctgtt caagcaggcg gggctcgacc ccaacaagcc gccgaccagc
     1561 ctggcggaga tccgcgagta cgccgacaag atctcggcgc tcggcggcga catcaagggc
     1621 tactacctgc ccggcaactg cgccggctgc aacatcttca ccgtcggccc gctgatgtgg
     1681 gccaccccgg agacgaccat cgaggctgcc acgtgcgagt ccgagccgct ggtcggcccg
     1741 ggcgtgcccg aggtcctgca gtgggcccgg gacatgatca aggccggcaa cgtccacgac
     1801 accgaccgcg ccgagaccgg cgagacgttc catctccagt tcggctccgg caaggtcggc
     1861 atgatgggca ccggcaactt caacatcacg ctggccaacg accagatgaa ggaccacccc
     1921 ttcgagttcg gcatcaccaa cctgccgggc aaggatccgg gctcctccgc atcgttcatc
     1981 ggcggcgacc tcgtcgtcgt gccgcagggc agcgagcgcg tcgacgacgc ggtcgagttc
     2041 atgaagttca tcctgtcgga caacgagcag gtcgagggct acgcgaagct gctgaacctc
     2101 acgacccgca gcgacatggc caacaacaag tacttccagg aagagccgct cgtccaggac
     2161 gtcgccaagg cgctcgaggt cggcacgacc ccgtacaccc tgacgttctt cgagcagatc
     2221 aactcgccgc agggtccctg gctgcagatg ctccagaagg cctactacac ggacgagccg
     2281 atcgactcga tcatcgcctc cgccaaggag cagatgaagc agatcgcctg ccagtcctag
     2341 agacgggacc ggctcctcct ccgggtcggg ccgcgacgac tcacctgagc cgtcgcggcc
     2401 cgattagcgc ctgacacgac gattaccatc cacctcgacc gacgacccgg cgccggcgcg
     2461 agcgaccctg gagagccatg ctggcgacgc gccggaacaa gctgatgggg ctcctgttcc
     2521 tgagtcccgc gctgctgttc gtgctggcgt tcaccgccta cccgctctcc cagctggtct
     2581 ggatgtcgtt caacagctgg tcgctgatca cgccgcccaa gttcatcggg accggcaact
     2641 tcacccgggc gttcagcgac gaccagttct gggtgtcgct cacctacagc ctcaagtaca
     2701 cgctgttcat cacgccgatc ctcatcatcg gcggctacct gattgcgctg ctggtcgccg
     2761 acaactcgcg catcaagcgg gtcacccgga cgatggtgtt catccccgtc gtcatcggcc
     2821 tcggcgtctc gagcctcctc tggtactggc tctacaaccc gctgttcggc ctgatcgaca
     2881 cggcgctgct cgacctcggt ctcatcagca agccgatcct gtggctgggc accgaagagg
     2941 gcacatcgac ctgggcgatc atcggctcgg tgacctggaa ggtcatcggg ttcggcatgc
     3001 tgctgttcgt ggcggcgatc caggggatcc cggccgaggt caacgaggcg gcggtcgtcg
     3061 acggggcgag cttccggcag cgcgtgtggc gcgtcaccgt gcccctcacg gcgccgacga
     3121 tcctcctggt cacgctgatc agcgtgatcg gctcgctgct cgccttcgac cagttctaca
     3181 tcatgacggc cgggcagccg ttcaaccaga cggcgacagc cgtgttctac gtctacctca
     3241 actcgttccc ctacctcaag ctcggctatg gggcggcctt gtcgctgctg atcgcgggcg
     3301 tggtcctcct gttcacggtc gtccagctgt ggttcacccg ccggagccgg caatgagcac
     3361 cgagacgctc gaggcggccc agccgcgcgc ggacactcgc tcgctgcgct cgcgcgcctg
     3421 gtcgggccgc aacaagtacc tcgtcggggc gctgtgcctc gcgatctgcg cggtgatggt
     3481 cgcgccggtc gtgttctcga tcctcgcgtc ggtcaagacg acggcggagg ccgccgccca
     3541 gccgccgacc tacctcccgg gcgagctgag cctcgacagc tactcccggc tgtggaccta
     3601 ccaggcgggg cttccggcgt acctcgccaa cagcgtcggc accgcgctcc tgaccatcgt
     3661 gatcgcgctc ggactgacga tcccggccgg ctatgcgctc gcgcgcttcc cgatcccgtt
     3721 caaggagccg ctgttcgtct tcctgctgct ggcgctgatc atcccgtacc aggcgctgct
     3781 gacgcccatg ttcctgatgt tcgtgaagct gaagctcacc aacacgatcc tcgggctggc
     3841 gatcctgcac acgacgatcc agctgccgtt cagcctgtac atcatgcgca acagcttcga
     3901 ggcggtcccc cgagagctcg aggaggccgc ctacatcgac gggggcagcc cgctgcaggt
     3961 cctgcggcgc gtcttcctgc cgatggtggt gccggccatc gtgaccgtcg ccctgttcgc
     4021 gttcatcacg tcgtggaacg agctgctggg ctcgctggtg atgaacagca aggagtccgc
     4081 gttcaccctg ccggtgatcc tggcggccgc gcgcaccgag accagcctcg gcggcaccga
     4141 ctggggcatg ctccaggcgg gcgtcaccat ctcgatcatc ccctgcatcc tcgtgtacgt
     4201 gctgctgcag aagtactacg tgtccggcct gctgagcgga gccgtcaagt gagggcggtg
     4261 ccggggacca cggcccgccg gggggaccgg gttgcgtgcg gcagccgcgc atgcaatcgt
     4321 atgctcgcgt tcgtattctc aatgcccacg ggagaagcgc agccgtgaac gcccgcgtcg
     4381 gcaacccggc ggcccgggtc gcgagtccca tcgcggtggt cgtgccctcg cccgacgcca
     4441 tcacgcgtgt gcgcccgctg ggcggccagg acgtgggcat cacgggcggg ctgtggcacg
     4501 aacggttgcg cgtgaaccgc gagcagacgc tgccgcacgt gttcggccag ctcgtcgagg
     4561 tgggcaacct gcacaacctg cgcctcgccg cgggtgccgg tggccagtac caggccctcg
     4621 ggatcatgtt cgatgggccg ttcccgttcc tcgactctga cgtgtacaag tggctcgaag
     4681 gggtcgcctg ggagctcggc cggacctggg acccggctat cgcggcgatg gccgacgagg
     4741 cgatcgacgc catcgcggcg gcccagcgcg aggacgggta cctgaacacg tttgtccagg
     4801 tgctcgcgcc gggccgcgag tacaaggacc tgcagttcgg ccacgagctg tattgcatcg
     4861 gccacctgat ccaggcgggg gtcgcctggc accgtgccct cggcgacgac cggctcctgg
     4921 cgatctcgcg ccgggccgcc gactcggtcg acgccgccct tggaccgaac ggggcgacgg
     4981 ggatcgacgg tcacccggag atcgagatgg ccctggtcga gctgtaccgt gcgaccggcg
     5041 aggcgcgcta cctcgagctc gcgcggctgc acgtcgagcg ccgcggtcac gggttgctgg
     5101 gcgccggccg gttcggggcg ccgtactggc aggaccacct gcccgtgcgc gaggcaacgg
     5161 acgtcgccgg ccacgtcgtc cggcagctgt acctcgatgc cggcgccgtc gatgtcgcca
     5221 ccgaaaccgg cgacacggac ctgctcgacg ccgtgcaccg gcgatggcgg gacatggtcg
     5281 cgacacgctc gtacctcacc ggcggcgtcg gcagccggca cgagcacgag ggcttcggcg
     5341 acccgtacga gctgccaccc gatcgcgcct atgccgagac gtgcgcggcc atcgcgagca
     5401 ccatgctcgc ctggcgcctg ctcctcgcga ccggcgaccc ggactgcgcc gacgtcatcg
     5461 agcggacgat ctacaacggc gtgctctcgg gcgtgtccac cgacgggttg cgattcttct
     5521 acgtgaaccc gctccagcgc cggacgcacc gggtcgcggc cgactcgggc acgggcgagc
     5581 gggcaccgtg gtacgcctgc gcctgctgcc cgccgaacct cgtgcgcacg ttcggcagct
     5641 ggccgcagta cctcgcgacc acggacgacg acgggatcca gatccaccag tacgcagcgg
     5701 ccgagatccg cgcgacgctc ccgggcggcg acgtggccct cgcggtcgag acggactacc
     5761 cgtgggacgg cagggtgcgc atcaccgtac gccagacgcc cgacacgccc tggtcgctca
     5821 acctgcgggt cccgggctgg gtggatgccg gcagcgtcga ctggtacgcg gccgccgagg
     5881 gtgccgtcga tgcccatccc ccgagcccga tcgccgccgg cgaccggctc gtcacctgtt
     5941 cccgcgtgtg gcacgccggc gacgcggtcg tcctcgacct cgacatgccc ggtcgcgtca
     6001 cgcggccggc gccgcaggtc gatgccatcc ggggctgcgt
//
LOCUS       DBNHXO010000313         1829 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1478623, whole genome shotgun sequence.
ACCESSION   DBNHXO010000313 DBNHXO010000000
VERSION     DBNHXO010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1478623"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="AC162A_06580"
     CDS             <1..911
                     /locus_tag="AC162A_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010543.1"
                     /translation="NPTDPEATADLPTETYGYAASLVLARAIAERGGEDALRRVWAAA
                     QGRVGAYQPPTGAFGGEIDPETVDGPPDWRGLLDLLEAESGEDFTDLWREWVVRPDEA
                     DLLDARANARAEYARTVTLAGDWRLPRGVRDALRSWQFDAALRQLADARTVLAQRSAI
                     EGLAERGSMTLPATVQQHFQAGDMVGASTQAEAERNAMLSVLQAADARVHDDDVLSRI
                     GMLGENPEGALVVARTAFAAGDLDGARAAADDAYRAWTAAWQEGRRRALLVLATLTAV
                     LVLAAAVAGRARAARRNRTATIAAGS"
     gene            923..>1829
                     /locus_tag="AC162A_06585"
     CDS             923..>1829
                     /locus_tag="AC162A_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010544.1"
                     /translation="MTRIRRPATAGIVLAAALSILAAIVPPAAAPIAQVAPFLAAFDA
                     PVALAADDLAVSTKARYVVAPDGGKVRVVVDINVLNQKPNRVSGGVITRYFYDRVNLG
                     VQPEATRLRATQDGASVPVTSDKRKTFRLATIEFGDDLYFGDTTHLKLSFDLPAGDPR
                     SDSGVRVGPAFATFLAWAFGDKGSVRIEVPKPFDVDVTGQEMREQAAGDAKVFTATTA
                     DALGWYAWVNARNDDGLTREALELTGGERIIVRGWPEDSRWRRRVSAILTDSVPDLVG
                     RIGLPWPVDGPLNVLEVHTPLLEGYA"
BASE COUNT          249 a          683 c          663 g          234 t
ORIGIN      
        1 cgaacccgac ggatcccgag gcgaccgccg acctcccgac cgagacctac gggtatgccg
       61 cctcactcgt gctcgcccgc gccatcgccg agcgtggcgg ggaggacgcg ctgcgccgcg
      121 tctgggctgc cgcgcagggg cgggtgggtg cctaccagcc tccgaccggc gccttcggcg
      181 gcgagatcga ccccgaaacc gtcgacggtc cgcccgactg gcgcggcctg ctggacctgc
      241 tcgaggccga gtcaggcgag gacttcacgg acctgtggcg tgaatgggtg gttcgacccg
      301 acgaggccga cctgctcgac gcccgtgcca acgcacgcgc cgagtatgcg cgcacggtca
      361 cgctcgcggg cgactggcgg ctgccgcgcg gcgtgcgcga cgcgctccgc tcctggcagt
      421 tcgacgccgc gctgcgacag ctggcggacg cgcggaccgt gctggcccag cgaagcgcga
      481 tcgaggggct cgccgagcgc ggatcgatga cgctgccggc gaccgtgcag cagcacttcc
      541 aggcgggcga catggtcggg gcgtcgaccc aggccgaggc cgagcgcaac gcgatgctgt
      601 ccgtgctcca ggcagccgat gcccgggtcc acgacgacga cgtcctgagc cgcatcggga
      661 tgctgggcga gaaccccgag ggagccctcg tcgtcgcgcg caccgcgttc gccgccggtg
      721 acctcgacgg cgcccgggcc gccgccgacg atgcctaccg cgcctggacc gcggcgtggc
      781 aggagggccg gcgccgcgcc ctgttggtgc tcgccaccct gaccgccgtc ctcgtgctcg
      841 ccgccgccgt cgcgggacgg gcccgcgccg cgcgtcggaa tcgtacggcc accatcgcag
      901 ccggctcctg accgcaatgc cgatgacgcg catccgccgg cccgccaccg ccgggatcgt
      961 gctcgccgcc gcgctgtcca tcctcgcggc gatcgtgccg cccgcggcag cgcccatcgc
     1021 gcaggtcgcg ccgttcctcg ccgccttcga cgcgcccgtc gccctcgcgg ccgacgacct
     1081 cgcggtcagc acgaaggcgc gctacgtcgt cgcgcccgac ggtggcaagg tccgcgtggt
     1141 cgtcgacatc aacgtcctca accagaagcc gaaccgggtc tcggggggcg tgatcacccg
     1201 ctacttctac gatcgcgtga acctcggcgt gcagcccgag gcgacgcgcc tgcgggcgac
     1261 ccaggacggt gcgagcgtcc cggtgaccag cgacaagcgg aagacgttcc ggctggcgac
     1321 gattgaattc ggcgacgatc tctacttcgg cgacacgaca cacctgaagc tgtcgttcga
     1381 ccttcccgcg ggcgacccgc gctcggacag cggcgtgcgg gtcgggcccg cgttcgcgac
     1441 gttcctcgcc tgggcgttcg gcgacaaggg cagcgtgcgg atcgaggtcc cgaagccgtt
     1501 cgacgtcgac gtcacgggcc aggagatgcg cgagcaggcc gcgggcgacg ccaaggtgtt
     1561 caccgcgacg accgccgacg cgctcgggtg gtacgcgtgg gtcaacgcgc gcaacgacga
     1621 cgggctgacg cgcgaagccc tcgagctcac cggcggcgag aggatcatcg ttcgcggctg
     1681 gccggaggac tcgcgctgga gacggcgggt gtccgcgatc ctcactgaca gcgtgcccga
     1741 cctcgtcggg cggatcgggc tcccatggcc ggtcgacggg ccgctcaacg tgctcgaggt
     1801 ccacacgccg ctgctcgagg ggtacgcgg
//
LOCUS       DBNHXO010000314         3289 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4652147, whole genome shotgun sequence.
ACCESSION   DBNHXO010000314 DBNHXO010000000
VERSION     DBNHXO010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4652147"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="AC162A_06590"
     CDS             <1..365
                     /locus_tag="AC162A_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010545.1"
                     /translation="VGLPPGSRHELGALAFAAALRRRGIGVLYLGSDVTVDGWVDALA
                     RTRAKAAVVGVITDDDRESAADVVAALLAHDVPLVAVGGAASATPLSARSGGAVQIPD
                     RVVEAAASIAEAVGRRGR"
     gene            complement(366..1328)
                     /locus_tag="AC162A_06595"
     CDS             complement(366..1328)
                     /locus_tag="AC162A_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5010546.1"
                     /translation="MATVVDDVLEVSVVGSFSRIGPAVRARIFRWTAPSPGAMTGRTV
                     AITGPTSGLGLATTWALAALGARVVLIGRSGRRLSALATALDVAFGEERFPTVVADMG
                     DRASVRDAVERIVASERRLDVLIDNAGAIFPERRESPDGFEATFALMVVGPFALESGL
                     EPLLRGTPGSRVIAVTSGGMYTQQLPLDDPAYRRGDYSGPRAYARAKRAQVALMREWA
                     RRTGGSIAFTAMHPGWSDTPGLAESLPGFYRVMRPLLRTPEQGMDTIVWLATHPEPAA
                     INGRLFLDRRPRPFDRVPSTRLSRPERRRLWELVAGMASGSDPG"
     gene            1417..1863
                     /locus_tag="AC162A_06600"
     CDS             1417..1863
                     /locus_tag="AC162A_06600"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5010547.1"
                     /translation="MTTIREHIETSLPLDDAFAFIADFANAERWDPGVASSVRANPGP
                     VGVGARYRLGIRMRGRVVPMDYEVTTFDPPNLVVLEGTGSGVAATDEIRFTAHEGGTG
                     IDYVADIRLVGRMRLLAPFAGGALARIGRDARDGMQRALDRRAVGA"
     gene            1870..3141
                     /locus_tag="AC162A_06605"
     CDS             1870..3141
                     /locus_tag="AC162A_06605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5010548.1"
                     /translation="MDVAIVGSGISGLAAAYALRADHRVTVFEQDRQAGGHVKTVAVD
                     VGGGAVAVDTGFIVYNERTYPEFVRLLAELGVETQASDMSFGLACEACGLAYSSRGAG
                     GFFPNARTLGRFGQWRMLADIRRFYRDARAVLEDSSTATATLGEWLDERGYGRPFRDH
                     FLVPITSAVWSTAATRVLEFPVVYLLRFLDNHGLIGYGNAPQWRVVRGGSRAYVDRLV
                     ASLPAGSVRTDSPVAEIRRDLFGVTVTTRDRGRERFDAVVVATHADDALRLLTDADAY
                     ERRVLGGFEYSTNAVVLHTDERLLPASPRARASWNVRTGDCRRPGDALVMTYDMNRLQ
                     SLEGDVRYCVSVNPGPELRAELVIEERAFAHPTYTFRTLKAQAGMRDLQGQRRTWFAG
                     AHLGYGFHEDGCRSGFEAAAMVRDAAERVAA"
     gene            3138..>3289
                     /locus_tag="AC162A_06610"
     CDS             3138..>3289
                     /locus_tag="AC162A_06610"
                     /inference="COORDINATES: protein motif:HMM:NF018770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HIR5010549.1"
                     /translation="MRSHLLEGKVRHRRARPFTYGLEHDVWYAALDLSELDALDRRLR
                     LFGRNRR"
BASE COUNT          449 a         1206 c         1161 g          473 t
ORIGIN      
        1 tcgtcggcct gccacccggg tcgcgccacg aactcggcgc gctcgccttc gccgcggcgc
       61 tgcggcggcg cggcatcggc gtgctgtacc tcggctcgga cgtcacggtc gacggatggg
      121 tcgacgccct cgcacgcacg cgcgccaaag cggccgtcgt cggcgtgatc acggacgacg
      181 accgcgaatc ggcggcggac gtggtggcgg ccctgctcgc gcatgacgtc ccgctcgtcg
      241 cggtcggcgg ggcggcctcg gccactcccc tgtccgcgag gagcggaggt gcggtccaga
      301 tcccggaccg cgtcgtggag gccgcggcat ccatcgccga ggcggtgggt cgccggggac
      361 gctgatcagc ccgggtcgga cccgctcgcc atcccggcca cgagctccca cagccgccga
      421 cgctcagggc gggacaggcg cgtcgagggc acccggtcga acggtcgtgg gcggcgatcc
      481 aggaacagcc ggccgttgat cgcggccggt tcgggatggg tcgcgagcca gacgatggtg
      541 tccatgccct gttcaggggt ccgcagcagc ggccgcatca cgcggtagaa gccgggcagc
      601 gactcggcga gaccgggcgt gtccgaccag cccgggtgca tggcggtgaa cgcgatcgag
      661 ccgcccgtcc gccgcgccca ctcgcgcatc agcgcgacct gcgcccgctt cgcgcgggca
      721 tacgcccgtg gcccggagta gtcgccccgg cggtaggcgg gatcgtcgag gggcagttgc
      781 tgcgtgtaca tcccgcccga ggtgaccgcg atcacccgtg agccgggcgt gccgcggagc
      841 aggggctcca ggcccgactc gagcgcgaac ggcccgacga ccatcagcgc gaacgtcgcc
      901 tcgaacccgt cggggctctc gcgccgctcc gggaagatgg cgccggcgtt gtcgatcaac
      961 acgtcgaggc gccgctccga cgcgacgatc cgctccactg cgtcgcgcac cgacgcgcgg
     1021 tcgcccatgt ccgcgaccac cgtggggaac ctttcttcgc cgaacgccac atcgagcgcc
     1081 gtggccagtg cgctcaagcg ccgtccgctg cggccgatga ggacgacccg cgcgccgagg
     1141 gcggcgagcg cccaggtggt cgccaggccg aggcccgacg tcggcccggt gatcgccacg
     1201 gtccggccgg tcatcgcccc cggcgagggt gccgtccagc ggaagatccg cgcccggacg
     1261 gcaggcccga tccggctgaa gctgccgacg accgagactt cgagcacgtc atcgacgacc
     1321 gtcgccaacc tgtgacggac cattgacacg gtcccgggag gagcggtact gtgcgcatct
     1381 aacattagac aatcactata caagcgatcg agccccatga ctaccatccg cgagcacatc
     1441 gagacctccc tccccctcga tgacgcattt gccttcatcg ccgacttcgc caatgcggag
     1501 cgctgggatc ccggcgtcgc ctcatcggtc cgcgccaacc ccggtcccgt cggcgtcggc
     1561 gcacggtacc gcctcggtat ccgcatgcgc gggcgtgtcg tcccgatgga ctacgaggtc
     1621 accaccttcg atcccccgaa cctggtcgtg ctcgagggca cgggcagcgg tgtcgcggcc
     1681 acggacgaga tccggttcac cgcgcacgag ggcggcacgg gcatcgacta cgtcgccgac
     1741 atccgcctcg tcggccggat gcggctgctg gccccgttcg ccggcggcgc cctcgctcgc
     1801 atcggacgcg acgcccgtga cggcatgcag cgggcgctcg accgccgcgc cgttggggcc
     1861 tgacgaatcg tggacgtcgc catcgtcggc tccgggatca gcggcctagc ggccgcctac
     1921 gcgctgcgcg cggatcaccg ggtgaccgtg ttcgagcagg atcgccaggc cggcggccac
     1981 gtcaagaccg tggcggtcga cgtcggaggc ggggccgtcg cggtcgacac cggcttcatc
     2041 gtctacaacg aacggacgta tcccgagttc gtgcgcctcc tggccgagct cggcgtcgag
     2101 acccaggcga gcgacatgtc cttcgggctc gcgtgcgagg cctgcgggct ggcctacagc
     2161 tcgcgcggcg ccggcggctt cttcccgaac gcccggaccc tcggccgttt cggccagtgg
     2221 cggatgctcg ccgacatccg gcgtttctac cgcgatgccc gggccgtgct cgaggactcc
     2281 tcgacggcca ccgccaccct gggcgagtgg ctggacgagc gagggtatgg ccggccgttc
     2341 cgcgaccact tcctggtgcc catcacgtcg gccgtctggt cgaccgctgc cacccgcgtc
     2401 ctcgagttcc cggtcgtcta cctgctccgc ttcctcgaca accacgggct catcggctac
     2461 ggcaacgcgc cgcagtggcg ggttgtccgc ggcggctcgc gcgcctacgt cgaccgcctc
     2521 gtcgcgtcac tgccggcggg ctcggtgcgc acggacagcc ccgtcgcgga gatccggcgc
     2581 gacctgttcg gggtgaccgt caccacgcgc gatcgcgggc gcgaacggtt tgacgccgtg
     2641 gtcgtcgcga cgcatgcgga cgacgctctg cggctgctga ccgatgccga cgcctacgaa
     2701 cgtcgcgtcc tcggtggctt cgagtactcg accaatgcgg tcgtgcttca caccgatgag
     2761 cggctcttgc cggccagccc gcgtgcgcgc gcgtcatgga acgtccgaac cggcgattgc
     2821 cgtcggccgg gcgatgccct ggtgatgacg tacgacatga accggctgca gtcgctcgag
     2881 ggtgatgtcc ggtactgcgt ctcggtgaac ccgggacccg aactccgcgc ggagctggtc
     2941 atcgaggagc gcgcgtttgc gcacccgacg tacacgttcc ggacgctaaa ggcgcaggcc
     3001 ggcatgcgcg acctccaggg tcaacggcgc acgtggttcg cgggcgcgca cctcggctac
     3061 gggttccacg aggacggctg ccggtcgggc ttcgaggccg ccgcgatggt ccgcgacgcg
     3121 gccgaacggg tggccgcatg aggtcgcacc tgctcgaggg caaggtccgc catcgcagag
     3181 cgcggccgtt cacgtatggg ctcgagcacg acgtctggta cgccgccctc gatctctcgg
     3241 agctggacgc gctcgatcgc cgcctgcggc tgttcggccg gaaccgccg
//
LOCUS       DBNHXO010000315         2011 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2867187, whole genome shotgun sequence.
ACCESSION   DBNHXO010000315 DBNHXO010000000
VERSION     DBNHXO010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2867187"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="AC162A_06615"
     CDS             <1..440
                     /locus_tag="AC162A_06615"
                     /inference="COORDINATES: protein motif:HMM:NF036831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5010550.1"
                     /translation="EDDLIAILDRTTGSDAPTAQKAATAVEVVEMQRLARAVPIAPHI
                     TAYAVSLLAATHPDNRRAPGLVRQYVRYGGSPRGAQALVTAGKILALMDGRFNVSIDD
                     VRAAALPALRHRVILNFEGEAEGITTEATVRAILDEVAPPAVE"
     gene            460..1467
                     /locus_tag="AC162A_06620"
     CDS             460..1467
                     /locus_tag="AC162A_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR5010551.1"
                     /translation="MTASPTPPAETPDEAASPPSRPAAAAAAAARPGGVKAFLPNELD
                     PTVFDEGFLRQLERLGVLMKQPVRGGLKGGRRSVKRGQSVEFADYRDYTLGDDLRQLD
                     WNVYARLEKLFVKLFIEEEDVTISFLVDASPSMAFGRPEKLLFAKRAAAALGYIGLAS
                     EDRIVVTALSGRTARRQSGLRGSGRVLRLLANLSTIQPAEGPTDLVAAARHAAAMLSG
                     RGVVVLISDLLDPGADRVIRELAATGSELIVLHVLSPDELDPAVEGDLRLVDSESGEG
                     IDVTVDLATLDDYKARLAAWQEGLAELATKRRASYVPLSTDVALADLVFAELRRRRVV
                     G"
     gene            1473..>2011
                     /locus_tag="AC162A_06625"
     CDS             1473..>2011
                     /locus_tag="AC162A_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024909.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR5010552.1"
                     /translation="MPFLAPLALAGLVFFPLVIAMYLLKLRRDEAVVPSTLLWQRLVA
                     DVEANAPWQRLRRSLLLLLQLLLVLLLVFLAARPFLERPAGLAGDLVLVIDTSASMQA
                     TDVTPNRLEAAKAAAVDALKDLPAGGKVSVIAAGRTAKVIANGTSDIGRVRQAIASVT
                     PTADIGNLADALRLASALAA"
BASE COUNT          262 a          755 c          713 g          281 t
ORIGIN      
        1 ccgaggacga cctgatcgcg atcctcgatc ggacgacggg atccgatgcc ccgaccgcgc
       61 agaaggcggc cacggcggtc gaggtcgtcg agatgcagcg gctcgcacgg gcggtcccga
      121 tcgcgccaca catcaccgcg tacgccgtct cgctgctggc cgccacgcac ccggacaaca
      181 ggcgggcgcc ggggctggtc cgccagtacg tccgctatgg cggctcgccg cgcggtgccc
      241 aggccctcgt gaccgccggc aagatcctgg cgctgatgga cggccgattc aacgtgtcga
      301 tcgacgacgt gcgggccgcg gcgctgccgg cgctccgcca tcgggtgatc ctcaacttcg
      361 agggcgaggc ggagggcatc acgaccgagg ccaccgtgcg cgcgatcctg gacgaggtgg
      421 cgccgcccgc cgtcgagtag cggacccggc tcccttcgca tgaccgcctc ccccaccccg
      481 cccgccgaga cgccggacga ggcggcaagt ccgccgtccc ggccggctgc cgcagccgct
      541 gccgccgccc gtcccggcgg cgtgaaggcg ttcctgccca acgagctcga cccgacggtc
      601 ttcgacgagg gcttcctgcg ccagttggag cgcctcggcg tgctgatgaa gcagccggtg
      661 cgcggcgggc tcaagggcgg gcggcggagc gtcaagcgcg gccagtccgt ggagttcgcc
      721 gactaccgcg actacaccct gggcgacgac ctgcgccagc tcgactggaa cgtctacgcg
      781 cggctcgaga agctgttcgt caagctgttc atcgaggaag aggacgtcac gatctcgttc
      841 ctcgtcgatg ccagcccgtc gatggcgttc gggcgacccg agaagctgct gttcgccaag
      901 cgggccgcgg ccgcactcgg ctatatcggg ctggcatccg aagaccggat cgtcgtgacg
      961 gcgctcagcg ggcggaccgc ccgtcgccag agcggcctgc gcgggtcggg gcgggtgctc
     1021 cgcctgctcg cgaacctgtc cacgatccag cccgccgagg gcccgaccga cctcgtcgcc
     1081 gccgcgcgcc acgccgccgc gatgctctcg ggacggggag tcgtcgtgct catctccgac
     1141 ctgctcgacc cgggcgcaga cagggtgatc cgcgagctgg cggcgacggg ctcggagctg
     1201 atcgtgctcc acgtgctgtc gcccgacgag ctggacccgg ccgtcgaggg cgacctgcgc
     1261 ctcgtcgaca gcgagtcggg cgagggcatc gatgtcacgg tggacctggc gacgctcgac
     1321 gactacaagg cgcgcctcgc cgcctggcaa gagggcctgg ccgagctcgc caccaagcga
     1381 cgggcgtcgt acgtgccgtt gtccacggac gtggccctgg ccgacctcgt gttcgccgag
     1441 ctgcggcgcc ggcgcgtggt cgggtagccg cgatgccgtt cctcgcgccc ctggcgctcg
     1501 cgggcctcgt gttcttcccg ctggtcatcg cgatgtacct gctcaagctg cgccgcgacg
     1561 aggcggtcgt cccgtccacg ctgctgtggc agcggctggt cgcggacgtc gaggccaacg
     1621 cgccctggca gcggctgcgc cggagcctcc tgctgttgct ccagctgctg ctcgtgctgc
     1681 tcctcgtctt cctcgcggcg cggccgttcc tcgagcggcc cgccggcctg gcgggcgacc
     1741 tcgtcctcgt catcgacacc tcggcgtcga tgcaggcgac cgacgtgacg ccgaaccggc
     1801 tcgaggcggc gaaggcggcc gccgtcgacg ccctcaagga cctgcccgcg ggcggcaagg
     1861 tcagcgtgat cgcggccggc cggaccgcca aggtcatcgc caacggcacc tcggacatcg
     1921 gccgggtcag gcaggccatc gcgagcgtca cccccaccgc ggacatcggc aacctcgccg
     1981 atgcgctgcg gctcgcctcc gcgctcgccg c
//
LOCUS       DBNHXO010000316         2194 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1874516, whole genome shotgun sequence.
ACCESSION   DBNHXO010000316 DBNHXO010000000
VERSION     DBNHXO010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1874516"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="AC162A_06630"
     CDS             complement(<1..559)
                     /locus_tag="AC162A_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5010553.1"
                     /translation="MTIVEPAGERVGRLLELAVDDLALIDRLRLVFSPGLNVLTGETG
                     AGKSLLIDALGLALGARADATLVRHGAENARVEALFDRLPEPLIAVREVGANGRSMAR
                     LDDDAVTAGRLALATGGLVEIHGQHDQQRLLDEGWQRDLLDAYGGHGEARALVAGTVE
                     RWRANRAALAELSMDPRELLRRLDLL"
     gene            complement(556..1398)
                     /locus_tag="AC162A_06635"
     CDS             complement(556..1398)
                     /locus_tag="AC162A_06635"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039482.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR5010554.1"
                     /translation="MNVGRIGFAYNPTIEQAIELGERAAGWCRMREIDQWAAAADDTA
                     ALREMLVGTDVLVVLGGDGTFLRAVQAVIAVDVPILGINLGKVGFLSKAETDELEAVL
                     EKLRTGAFELRERMGLGGAILPAGRPATGTFTALNDIVIARGSLARVVRMSVSIDESH
                     LATFIADGLVVSSPTGSTGYSFSAGGPILDPQSRNLVVTPIAGYLSAIRSIVVSPHQL
                     VRCQVLEADEALVSIDGREDHRIEVGDVVEVRALERPIRFVEPAGTMPFWDLLRTKVQ
                     LLPS"
     gene            complement(1395..2192)
                     /locus_tag="AC162A_06640"
     CDS             complement(1395..2192)
                     /locus_tag="AC162A_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR5010555.1"
                     /translation="MLVNRGLAESRARAQALVMAGQVRVGDGDSARTDLKAGDLVATD
                     ARLTVAAGQRWVSRGAHKLIAALDAFGIAVEGRVGVDVGASTGGFTDVLLERGASRVY
                     AVDVGYGQLAERLRQDPRVVSMERMNARTLTADTLPEPADVAVVDVSFISLGLVLGPI
                     RSVLRDGQGPIVALVKPQFEAGRAEAKGGVVRDPEVHRRVIGEVTARAAELGLGTRAV
                     IASPIQGPEGNREFLVHLQAGPSCAEVGERIDEAVAAAWAPGPGSTT"
BASE COUNT          302 a          851 c          765 g          276 t
ORIGIN      
        1 ccaggaggtc gagccgccgt agcagctccc gcgggtccat gctcagctcg gcgagcgcgg
       61 cccggttcgc ccgccagcgc tcgacggtgc ccgccaccag cgcgcgcgcc tcgccgtgcc
      121 ccccgtaggc gtcgagcagg tcccgctgcc agccctcgtc gagcagccgc tgctggtcgt
      181 gctgaccgtg gatctcgacc aggccgccgg ttgccagcgc cagccgcccc gcggtcaccg
      241 cgtcgtcgtc gagccgggcc atcgaccgcc cgttcgcccc cacctcgcgg acggcgatca
      301 ggggctccgg cagccggtcg aacagcgcct ccacccgcgc gttctccgcc ccgtggcgga
      361 ccagcgtcgc gtccgcgcgg gcccccagcg cgagacccag ggcatcgatc agcaggctct
      421 tgcccgcccc ggtctcgccg gtcaggacgt tgaggcccgg cgagaacacc aggcgcagcc
      481 ggtcgatcag cgcgaggtcg tccaccgcga gctcgagcag ccgtccgacg cgctcccctg
      541 ccggctccac gatggtcacg acggcagcag ctggaccttg gtccgcagca ggtcccagaa
      601 cggcatcgtg ccggcgggct cgacgaaccg gatcgggcgc tccagggcgc gcacctcgac
      661 cacgtcgccg acctcgatcc ggtggtcctc ccgcccgtcg atcgacacca gcgcctcgtc
      721 ggcctcgagg acctggcagc ggaccagctg gtgcggcgac acgacgatcg agcggatcgc
      781 cgagaggtag ccggcgatcg gggtcacgac gaggttgcgg ctctgcgggt cgaggatcgg
      841 gccgccggcc gagaacgagt agccggtcga tcctgtgggg ctggagacca cgaggccgtc
      901 ggcgatgaac gtggccaggt gcgactcgtc gatcgagacg gacatccgca cgacccgtgc
      961 cagcgagccg cgcgcgatga cgatgtcatt gagcgcggtg aaggtacccg tggcggggcg
     1021 cccggccggc aggatcgcgc cgccgaggcc catccgctcg cgcaactcga acgcgcccgt
     1081 gcgcagcttc tcgaggaccg cctcgagctc gtcggtctcg gccttggaca ggaagcccac
     1141 cttgcccagg ttgatgccca ggatcgggac gtcgacggcg atcaccgcct ggaccgcgcg
     1201 cagaaacgtg ccgtcgccgc ccagcacgac gaggacgtcg gtgccgacga gcatctcgcg
     1261 cagggccgct gtgtcgtccg ccgccgcggc ccactggtcg atctcgcgca tccggcacca
     1321 gccggcggcc cgttcaccga gctcgatcgc ctgctcgatc gtggggttgt aggcgaagcc
     1381 gatgcggccg acgttcacgt ggtcgacccc ggcccgggcg cccaggcggc ggcgaccgcc
     1441 tcgtcgatcc gctctccaac ctccgcgcac gacgggcctg cctggaggtg gacgaggaac
     1501 tcgcggttgc cctccggacc ctggatcggc gacgcgatga ccgcgcgcgt cccgaggccg
     1561 agctccgctg cccgtgcggt cacctccccg atgacccggc gatggacctc gggatcgcgt
     1621 acgaccccgc ccttcgcctc cgcccgcccg gcctcgaact ggggcttgac cagggcgacg
     1681 atcgggccct ggccgtcgcg cagcaccgag cggatcgggc cgagcacgag gccgagcgag
     1741 atgaacgaca cgtcgacgac cgcgacgtcg gccggttcgg gcagcgtgtc cgcggtcagg
     1801 gtccgggcgt tcatccgctc catcgacacg actcgcgggt cttgccggag ccgctcggcc
     1861 agctgcccgt acccgacgtc gacggcgtag acccggctgg cgccgcgctc gagcagcacg
     1921 tcggtgaacc cgcccgtcga ggcgcccacg tcgacaccga cccggccctc gaccgcgatc
     1981 ccgaaggcat ccagcgccgc gatgagcttg tgggcgccgc gactcaccca gcgctgaccc
     2041 gccgcgaccg tgagacgggc gtcggtagcc accaggtcgc cggccttgag gtccgtgcgc
     2101 gcgctgtcgc cgtcgcccac ccggacctgg cccgccatca cgagcgcctg ggcccgggcg
     2161 cgcgattccg cgagcccgcg gttcacgagc agga
//
LOCUS       DBNHXO010000317         3383 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2072424, whole genome shotgun sequence.
ACCESSION   DBNHXO010000317 DBNHXO010000000
VERSION     DBNHXO010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2072424"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="AC162A_06645"
     CDS             complement(<1..659)
                     /locus_tag="AC162A_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR5010556.1"
                     /translation="MTTPWFGLHLPNYTFPDRPPAQRFEATIEQAKAAEAAGFSLVTV
                     MDHLNQIPGIGQQDEPMLEGWSVLAALARETKSVRLGTLVTGVTYRNPALLAKTATTL
                     DVISGGRAIFGLGAAWFEAEHDAFGFEFPPIGERMDRLDEALTIAKAMFTQDRPTFEG
                     RHYQVTSILNVPKPIQPGGPRILVGGGGEQRTLKIAAEHADMTHWFPLGREALQHKKD
                     VL"
     gene            complement(758..1192)
                     /locus_tag="AC162A_06650"
     CDS             complement(758..1192)
                     /locus_tag="AC162A_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017945239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HIR5010557.1"
                     /translation="MDWKLEVVVIPVTDVDRAKAFYSEQIGFNVDVDHDMGTSRIVQM
                     TPRGSGCSVTIGRGLNDAVPGSVKGMQLCVGDIEAARAELVERGVDVSPIRHVGPEGW
                     ADGNGGEWNAFIFFDDPDGNSWAVQESPTLRAELKAETAAAS"
     gene            complement(1205..1696)
                     /locus_tag="AC162A_06655"
     CDS             complement(1205..1696)
                     /locus_tag="AC162A_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5010558.1"
                     /translation="MGTTKTTAPAGVPFIDLSREFDAPRDVVWRAWTEPALIKQWLGP
                     DRYEMIVDEYDVRDGGRYRYIHKDPDSGNEYGFHGVFHGTPSPDSFVQTFEFEGAPGH
                     VSLDKLVLEDLGGGRTRVVVHSVYQSVEARDAMVASGMETGLNEGFARLDNVLETLKS
                     PVH"
     gene            complement(1696..2067)
                     /locus_tag="AC162A_06660"
     CDS             complement(1696..2067)
                     /locus_tag="AC162A_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5010559.1"
                     /translation="MAVDQLSIVFGALADPTRRAILARLTSGDANVAELAAPFQISQP
                     AVSKHLKVLEEAGLISRHREATARLSHLEAAPLREATQWLVTYHEYWDESFDRLDALL
                     ASLQQRAKAGWQPATDEKETT"
     gene            2415..2975
                     /locus_tag="AC162A_06665"
     CDS             2415..2975
                     /locus_tag="AC162A_06665"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF013989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR5010560.1"
                     /translation="MSNLTYLLNVSLDGFIETTDHSLDWTAIDEELHYWFNERTRGAA
                     ASIYGRRMYDLMTAYWPTAASDPDATPAMLEYAEIWNAMPKIVFSRTLDSADHGFRVV
                     RGDIGDELANLRKEFDGEIEVSGPNIASQYIERGLVDEYCLVVHPVILGAGTPFFPTI
                     ATPIRLRQTASKRFESGVLALTYERA"
     gene            complement(2979..>3383)
                     /locus_tag="AC162A_06670"
     CDS             complement(2979..>3383)
                     /locus_tag="AC162A_06670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5010561.1"
                     /translation="MHWLAEVDGSIVAHASVVERRLEAGDRALRTGYLEAVATLPAFE
                     RHGYGSMVVRAAGKYLLPRFELGALSTSVPGFYLRLGWEAWLGPTLVRTVSGPERTED
                     DDGGIFILRTPSTPALTLTDPLICDWRSGDVW"
BASE COUNT          525 a         1179 c         1119 g          560 t
ORIGIN      
        1 aggacgtcct tcttgtgctg gagcgcctcg cgaccgagcg ggaaccagtg cgtcatgtcg
       61 gcgtgctcag cggcgatctt gagcgtccgc tgctcgccac ccccaccgac gaggatccgc
      121 gggccgccgg gctgaatggg cttcgggacg ttgaggatgc tcgtcacctg gtagtggcgg
      181 ccctcgaacg tcggccggtc ctgggtgaac atcgccttcg cgatcgtcag cgcctcgtcg
      241 aggcggtcca tccgctcgcc gatcggcggg aactcgaacc cgaaggcgtc gtgctcggcc
      301 tcgaaccagg cggcgcccag cccgaagatc gcgcgcccgc cggagatcac gtcgagcgtc
      361 gtcgccgtct tcgccagcag cgccgggttg cggtaggtca cgccggtcac gagggtgccg
      421 aggcggaccg acttcgtctc gcgcgcgagc gccgccagca ccgaccagcc ctcgagcatc
      481 ggctcgtcct gctgcccgat ccccgggatc tggttgaggt ggtccatcac ggtgacgagg
      541 ctgaacccgg ccgcctcggc ggccttggcc tgttcgatcg tcgcttcgaa ccgctgcgcc
      601 ggcggccgat cggggaaggt gtagttgggc aggtggagtc cgaaccaggg tgtcgtcatg
      661 tgatcaagga tgcccccgat gccacgctcc gcgcgaacgg atgccccgac ccggcagtgc
      721 cggatcgggg cgggagcggc gcgagttcgc ggtcaggcta ggaggccgcg gcggtctccg
      781 ccttgagctc ggcgcggagc gtcgggctct cctggacggc ccacgagttg ccgtccggat
      841 cgtcgaagaa gatgaaggcg ttccattcgc cgccgttgcc gtccgcccat ccttccggac
      901 cgacgtgacg gatcggtgac acgtccaccc cgcgctcgac gagctcggcc cgcgcggcct
      961 cgatgtcgcc gacgcacagc tgcatgccct tgaccgaacc gggaacggcg tcgttcaggc
     1021 cccggccgat cgtcaccgag cagccggatc cgcgtggcgt catctgcacg atgcggctcg
     1081 tgcccatgtc gtggtcgacg tcgacgttga acccgatctg ctcgctgtag aaggccttgg
     1141 cgcggtcgac gtcggtcacg gggatgacga cgacctcgag cttccagtcc atcgatctct
     1201 cctgtcagtg aacgggtgac ttgagcgtct cgagcacgtt gtcgagtcgc gcgaagcctt
     1261 cgttgaggcc ggtctccatg cccgatgcga ccatcgcgtc gcgggcctcg accgactggt
     1321 acacggagtg gaccacgacc cgggtgcggc caccgccgag gtcctcgagc acgagcttgt
     1381 cgagcgacac gtgccccggg gcgccctcga actcgaacgt ctgcacgaac gagtccggcg
     1441 acggggtgcc gtggaacacc ccatggaagc cgtactcgtt gccgctgtct ggatccttgt
     1501 ggatgtagcg gtatcggccg ccgtcgcgga cgtcgtactc gtcgacgatc atctcgtacc
     1561 ggtcgggccc cagccactgc ttgatcagcg cgggctcggt ccaggcgcgc cagacgacat
     1621 cccgcggggc gtcgaactcg cggctgaggt cgatgaatgg cacgccggcg ggggccgtgg
     1681 tcttcgtggt tcccatcacg tggtctcctt ctcgtcggtc gccggttgcc agccggcctt
     1741 cgctcgttgc tggagggatg cgagcagcgc gtcgagtcga tcgaagctct cgtcccagta
     1801 ctcgtggtag gtaaccagcc actgcgtcgc ctcccgaagc ggcgccgctt cgaggtggct
     1861 gagccgggcg gtcgcctccc ggtggcgcga gatcagtccg gcctcctcga gcaccttgag
     1921 atgcttcgag accgccggct gcgagatctg gaacggcgcg gccagctcgg cgacattggc
     1981 gtcacccgat gtgagccgcg cgaggatcgc gcggcgggtc gggtccgcga gggctccgaa
     2041 cacgatgctg agctggtcaa cggccattca tcactcacag gctatataac tgattagtta
     2101 tatactagcg agccaccgcg ggcgtgtcaa gcccctgcga gaccaggctc atttcaccca
     2161 cggctccctg ctcccggcac atagtcaaag cacgactgct tgaccgggcg tggcgcgcgt
     2221 caattgcccg ttcatcggaa gcgaatggtt ggcgacacat cgtccccgtg cgactacctg
     2281 ggtcgagttc gagcgcccgc accatccgga tcgcgcctgg cccgcgacaa gtagtcgtcc
     2341 ctggactggt tggcgggatc gagggctcgc ggggacttgc gccaccgccc tcacctgcga
     2401 tacccctacg tgtcatgagc aacctcacct acctgttgaa cgtgtcgctc gacggattca
     2461 tcgagacaac ggaccactcg ctcgactgga ccgcgatcga cgaggagctc cactactggt
     2521 tcaacgagcg gacgcgcgga gccgcggcgt cgatctacgg ccggcgcatg tacgacctga
     2581 tgaccgcgta ctggcccacc gcggcgtcgg accccgacgc cacgcccgcc atgctcgaat
     2641 acgccgagat ctggaacgcg atgcccaaga tcgtcttctc gcgcaccctc gactcggcgg
     2701 accacggctt ccgcgtggtg cgcggcgaca tcggtgacga gctcgcgaac ctgcgcaagg
     2761 agttcgatgg cgagatcgag gtcagtggcc cgaacatcgc ctcgcaatac atcgagcgcg
     2821 gcctggtcga cgagtactgc ctcgtcgtcc acccggtgat cctcggcgcc gggacgccgt
     2881 tcttcccgac gatcgcaaca ccgatccgcc tccgccagac cgcttcgaag cggttcgagt
     2941 cgggcgtcct cgccctgacc tacgagcgtg cctgagcact accagacgtc gccgctgcgc
     3001 cagtcgcaga tcaacggatc ggtgagggtc agagcaggcg ttgagggcgt gcggaggatg
     3061 aagatcccgc cgtcgtcgtc ctcggtgcgc tccgggcccg acaccgtgcg cacgagtgtc
     3121 ggcccgagcc acgcctccca cccgagccgg aggtagaacc cggggacgct tgtcgagagc
     3181 gcaccgagct cgaaccgcgg cagcaggtac tttcccgcgg cgcggaccac catcgacccg
     3241 tagccatgtc gctcgaacgc cggcagcgtc gccactgcct cgagataccc ggtgcgcagc
     3301 gcgcggtccc ccgcctccag ccgtcgctcg accaccgacg cgtgcgccac gatcgaccca
     3361 tcgacctcgg ccagccagtg cat
//
LOCUS       DBNHXO010000318         1726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2470525, whole genome shotgun sequence.
ACCESSION   DBNHXO010000318 DBNHXO010000000
VERSION     DBNHXO010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2470525"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="AC162A_06675"
     CDS             complement(<1..287)
                     /locus_tag="AC162A_06675"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HIR5010562.1"
                     /translation="MEQLLALEMVRVTEAAAIASARFMGRGDRHEADRAATEAMRRAM
                     DETDIAGTIVIGEGERDEAPMLYIGERVGRHDEEAVEAVDIAVDPLEGTNLV"
     gene            complement(306..>1726)
                     /gene="purH"
                     /locus_tag="AC162A_06680"
     CDS             complement(306..>1726)
                     /gene="purH"
                     /locus_tag="AC162A_06680"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR5010563.1"
                     /translation="VASVSDLTEVPPLVGGQVRTFHPAIYAGILARRDVPEQLNELAD
                     HGLEVIDLVVVNVKPFAPEVGSGHVSIDEAIEMIDVAGAALLGAGARNAAGVVAVCDP
                     SHYGLVVDEMRTLGQVSAETRYRLAADAFSTVAAYYAEIAAYLNQIGGNSYPKRLAMV
                     LEKVADLPYGENPHQRAAFYRETTHRSGTLADVKQLHGDPPSFNNLLDLDAAYRIASD
                     YTAPTVVIAKHTDPVGIASGEELVEAYRRALDTDPVSSFGGIVGVNRELDGATAREIA
                     ANSYEAVIAPGFSQAARGILEAKPGIELLAVPASPIEGMRDYGIANVDFKRVAGGLLV
                     EALDAVDLDRGQLQVVTRRHPTLGELTDLLFAWRAVRHVRSNAIVLARNAATVGIGAA
                     QASRNVSVDIALRRAGDRAKLAVMASDAYFPFPDGIQVAANAGVTAIIQPGGSIRDEM
                     AIEVADRHHLAMVFTGRRHFRH"
BASE COUNT          235 a          593 c          644 g          254 t
ORIGIN      
        1 acgaggttgg tgccctcgag cggatccacg gcgatgtcga cggcctcgac ggcctcctcg
       61 tcgtggcgtc cgacgcgctc gccgatgtac agcatcggcg cctcgtcgcg ctcgccctcg
      121 ccgatcacga tggtcccggc gatgtccgtc tcgtccatcg cccggcgcat cgcctcggtc
      181 gccgcgcggt ccgcctcgtg gcgatcgccg cggcccatga agcgcgcgga cgcgatcgcg
      241 gcggcttcgg tgacccgcac catctcgagg gcgagcagct gctccactac tcgacgccca
      301 cgggcctaat ggcggaagtg ccgacggccg gtgaagacca tcgccaggtg atgccggtcg
      361 gcgacctcga tcgccatctc gtcgcggatc gaaccacccg gctggatgat cgcggtcacg
      421 cccgcgttcg ccgccacctg gatcccgtcc gggaacggga agtaggcgtc ggacgccatc
      481 accgcgagct tggcgcggtc cccggcccgg cggagcgcga tgtccaccga gacgttgcgg
      541 ctcgcctgcg cggcgccgat cccgacggtg gcggcgttgc gggccagcac gatcgcgttc
      601 gaccggacgt ggcggacggc gcgccaggcg aacagcaggt ccgtgagctc gcccagcgtc
      661 gggtggcggc gggtgacgac ctgcagctgg ccgcggtcga ggtcgacggc atcgagcgcc
      721 tcgaccagga gcccgccggc gacgcgcttg aagtcgacgt tggcgatccc gtagtcgcgc
      781 atgccctcga tcggcgacgc ggggacggcg agcagctcga tgcccggctt cgcctcgagg
      841 atgccgcgag ctgcctgcga gaagccgggg gcgatgaccg cctcgtagga gttggcggcg
      901 atctcccggg cggtggcgcc gtcgagctcg cggttcacgc ccacgatgcc gccgaacgac
      961 gacaccgggt ccgtgtccag ggcgcggcgg taggcctcga ccagctcctc gcccgacgcg
     1021 atgcccacgg ggtcggtgtg cttggcgatg accacggtcg gggccgtgta gtcggacgcg
     1081 atccggtagg cggcgtcgag gtcgagcagg ttgttgaacg acggcgggtc gccgtggagc
     1141 tgcttgacgt cggcgagggt gccggagcgg tgggtcgtct cgcggtagaa cgccgcgcgc
     1201 tggtgggggt tctcgccgta gggcaggtca gcgaccttct cgaggaccat cgccagccgc
     1261 ttgggatagc tgttgccgcc gatctggttg aggtaggcgg cgatctcggc gtagtacgcg
     1321 gcgaccgtgc tgaacgcgtc cgcggcgagc cggtaacgcg tctcggccga gacctggccg
     1381 agcgtccgca tctcgtcgac cacgagcccg tagtgcgacg gatcgcacac cgcgaccacg
     1441 cccgcggcgt tgcgggcgcc cgctccaagc agggcggccc cggcgacgtc gatcatctcg
     1501 atcgcctcgt cgatgctgac gtggccgctc ccgacctcgg gcgcgaacgg cttgacgttg
     1561 acgacgacga ggtcgatgac ctcaaggccg tggtccgcaa gctcgttcag ctgctccggg
     1621 acgtcgcggc gggcgaggat gccggcgtag atcgcgggat ggaaggtcct gacctggccg
     1681 ccgacgagcg gcgggacctc ggtgaggtcc gagacggacg caacct
//
LOCUS       DBNHXO010000319         1594 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1480150, whole genome shotgun sequence.
ACCESSION   DBNHXO010000319 DBNHXO010000000
VERSION     DBNHXO010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1480150"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="AC162A_06685"
     CDS             complement(<1..633)
                     /locus_tag="AC162A_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030544 - Hsp70 protein binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR5010564.1"
                     /translation="MTFQGDPYRELGVAPGASLNEIRSAYRRLAKKYHPDAAGDRALP
                     RFLAIQAAYERLVDSEGRLRPVTGAAGTARRASEAWRADPAKARASREAWRARRAGGT
                     TAGGPRPGPRPAADGSAKEAPPREKHTRRGSRKATPGSTTYDEAAETPLDPAWEGGSW
                     YGPSSQTYWTINPREYADPRKHGPEYQARARRATRPRTPDAPNDFAAADGP"
     gene            complement(650..>1594)
                     /locus_tag="AC162A_06690"
     CDS             complement(650..>1594)
                     /locus_tag="AC162A_06690"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR5010565.1"
                     /translation="LRSAMKPFGLIALLRAGGRREFDLTTEELAIMTSSHSGEDVHVR
                     TVQALFRRLAIPQSVLACGAEGMPLDALTASRLARDGERPSSLRHMCSGQHSVFILLA
                     KLGGWEPETYWQDDHPAQAAVREAIGAAFAVDPARIKTGVDNCGILTYAFPLREVARA
                     YAILADPEALPDADPRVPLAPYFREIRDAMIAFPELVAGTRDRLDTSLMKATGGRCVS
                     KSGAEGLRGVGILGGSRNGAEAPPSGIAIKIEDGDGKDGGGWGATVEALRQVGVVEGQ
                     ALRVLARYHRPPYLDPHGRQAAEAIPDFQLAPVGELVV"
BASE COUNT          184 a          586 c          606 g          218 t
ORIGIN      
        1 cggcccgtcg gccgccgcaa agtcgttcgg cgcgtcgggc gtccgcggcc tcgtcgcgcg
       61 tctggcccgt gcctggtact ccggtccgtg cttgcgggga tcggcgtact cccgcgggtt
      121 gatggtccag taggtctgcg aggacgggcc gtaccaggag ccgccctccc acgccgggtc
      181 gagcggggtc tcggcggcct cgtcgtaggt cgtcgagccg ggcgtggcct tgcgcgagcc
      241 gcgacgtgtg tgcttctcgc ggggcggcgc ttccttcgcg ctcccgtcgg cggcgggccg
      301 gggcccgggt cgcgggccgc cggccgtggt gccacccgcg cggcgcgccc gccacgcctc
      361 ccgcgacgcc cgcgccttcg ccggatcggc gcgccaggcc tcggacgcgc gtcgggccgt
      421 ccccgccgcg cccgtcaccg gccgcagccg gccctcggag tcgaccagcc gctcgtaggc
      481 ggcctggatc gcgaggaagc ggggcagcgc gcggtcgccg gcggcgtccg ggtggtattt
      541 cttcgccagg cggcggtacg ccgagcggat ctcgttgagc gacgcgcccg gggcgacgcc
      601 gagctctcgg tacggatcgc cctggaaggt catgctgagc cgcggtccgt cagacgacga
      661 gctcgcccac cggcgcgagc tggaagtcgg ggatggcctc cgcggcctgg cggccatgcg
      721 ggtcgagata cgggggccgg tgataccggg ccaggacacg cagcgcctgc ccctcgacca
      781 cgccgacctg gcgcagggcc tcgaccgtcg cgccccatcc cccgccgtcc ttgccgtcgc
      841 catcctcgat cttgatcgcg atgccggatg gcggcgcctc cgcgccgttg cgcgagccgc
      901 cgagaatgcc gacgccgcgc agcccctctg cgccgctctt gctcacgcac cggccgccgg
      961 tcgccttcat cagcgacgtg tcgaggcgat cgcgcgtccc ggcgacgagc tcggggaagg
     1021 cgatcatggc gtcgcggatc tcgcggaagt acggggcgag cgggacgcgc ggatcggcgt
     1081 cgggaagggc ctccgggtcc gcgaggatcg cgtacgcccg ggcgacctcg cgcagcggga
     1141 aggcgtaggt caggatgccg cagttgtcga cgccggtctt gattcgggcc ggatcgacgg
     1201 cgaacgccgc cccgatggcc tcgcgaacgg ccgcctgggc cggatggtcg tcctgccagt
     1261 acgtctccgg ctcccagccc ccgagcttcg cgagcaggat gaacaccgag tgctgcccgg
     1321 agcacatgtg ccgcagcgac gagggtcgct cgccgtcacg cgccaggcgg gaggcggtca
     1381 gcgcgtcgag gggcatcccc tccgcgccgc aggcgagcac ggactgcggg atcgcgagcc
     1441 gcctgaacag cgcctggacc gtccgcacgt ggacgtcttc accggagtgg ctcgacgtca
     1501 tgatcgcgag ctcttcggtc gtcaggtcga actcgcgtcg gccgccggcc cggaggaggg
     1561 cgatcaggcc gaacggcttc atcgcggagc ggag
//
LOCUS       DBNHXO010000320         1842 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1676575, whole genome shotgun sequence.
ACCESSION   DBNHXO010000320 DBNHXO010000000
VERSION     DBNHXO010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1676575"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..765
                     /locus_tag="AC162A_06695"
     CDS             28..765
                     /locus_tag="AC162A_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5010566.1"
                     /translation="MYGGPGPYGVTAPRILIVDDDPNLLVILADQLRLDGYQVQTARD
                     GDEALRRLSQAWPDLLIIDMLMPRMDGLTLAREVKARADLPIIVLSAIDTADSKADLL
                     DEVAEDYVTKPYHYPELRARITRVLRRLGDRVPRQRLDLGPNLALELHRREAIVGGNP
                     VSLTPTESRLLYALAANLGQTVTTDTLLARGWADTEDADPSYVWVTMRRLRQKVEVDP
                     NKPVHLLTVRGIGYRLVAASGPTSGTR"
     gene            767..>1842
                     /locus_tag="AC162A_06700"
     CDS             767..>1842
                     /locus_tag="AC162A_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012876.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR5010567.1"
                     /translation="MSQADLTANDEPADAADAAVHAAPDGSASGPAAAEGGRLSERIS
                     RVDAARRASEAAANDDGRPIDGTAVYRGSGLGFRTRLTLALIAAAVLPVAGFGIVVLI
                     VTGTTGTDTSVARVLLLAVAVSVVFAVLLAALLAADLGAPLRAIARSVEQVSAGEDPG
                     RLELPGDDEFSRLAESHNRLARDLRRRNRELREIVIALEATSLSERPEIVIRRAATQA
                     RSAFGMIDCELLLVDAREIPTEEIVPGDPVPVRANLVAAGEPIGIVVGHLPATRNWER
                     ADQDLLELFAIEVSAAIRNAQLYARVEDQNRRLRELDEAKDDFLRGVSHNLQTPLASI
                     RGYAQQLAADTPDRRLGIITEQADR"
BASE COUNT          261 a          692 c          646 g          243 t
ORIGIN      
        1 cccgattcgc gctgatatcc tcgcggcatg tacggcggcc ccggcccgta cggcgtcacg
       61 gcgccgcgga tcctgatcgt tgacgacgac cccaacctgc tcgtcatcct cgccgatcag
      121 ctccggctgg acggctacca ggtccagacg gcgcgggacg gcgacgaggc gctccgacgc
      181 ctgtcgcagg cctggcccga tctcctgatc atcgacatgc tgatgccgcg catggacggg
      241 ctgacgctcg cgcgcgaggt caaggcccgc gccgacctgc cgatcatcgt gctgtcggcg
      301 atcgacaccg ccgactccaa ggccgacctg ctcgacgagg tcgccgagga ctacgtcacc
      361 aagccctacc actacccgga gctgcgggcg cgcatcaccc gggtcctgcg acgactgggc
      421 gatcgcgtgc cacgccagcg cctggacctc ggccccaacc tcgccctcga gctgcaccgg
      481 cgcgaggcga tcgtcggggg caaccccgtg tcgctcacgc ccaccgagtc gcggctcctg
      541 tacgcgctcg cggccaacct cggccagacc gtgaccacgg acacgctgct cgctcgtggc
      601 tgggccgaca cggaggacgc cgacccgagc tatgtctggg tgacgatgcg gaggctgcgc
      661 cagaaggtgg aggtcgaccc taacaagccc gtccacctgc tcaccgtgcg cggcatcggc
      721 taccggctgg tggcggccag cgggcccacg agcgggaccc gatgaggtga gccaggccga
      781 cctgacggcg aacgatgagc ccgcggatgc agccgacgcg gcggtccacg cggcgcccga
      841 cggatcggcg agtggccctg ccgcagccga gggcgggcgc ctctcggagc ggatctcacg
      901 cgtcgacgcg gcgcgccggg cgtccgaggc cgccgccaac gacgacgggc ggccgatcga
      961 tgggaccgcg gtctaccgcg ggtcgggtct cgggttccgg acccggctca cgctcgcgct
     1021 gatcgcggcc gccgtcctgc cggtggccgg cttcggcatc gtggtcctga tcgtgaccgg
     1081 caccacgggg accgacacct ccgtcgcccg ggtgctgctg ctcgcggtgg ccgtctcggt
     1141 cgtgttcgcc gtcctgctgg ccgcgctcct cgcggccgac ctgggcgccc cgttgcgggc
     1201 catcgcgaga tccgttgagc aggtctcggc gggcgaggac cccggccgcc tggagctgcc
     1261 cggagacgac gagttcagcc gcctcgccga aagccacaac cgcctcgccc gggacctgcg
     1321 gcgacgcaac cgggagctgc gtgagatcgt catcgcgctc gaggccacca gcctctccga
     1381 gcgtcccgag atcgtcatcc gccgtgcggc cacgcaggcc cggagcgcct tcgggatgat
     1441 cgactgcgag ctcctgctcg tcgatgcccg cgagatcccc accgaggaga tcgttcccgg
     1501 ggatccggtg cccgtccggg cgaacctcgt cgcggccggc gagccgatcg ggatcgtggt
     1561 cggccacctc ccggccaccc ggaactggga gcgcgcggac caggacctgc tggagctgtt
     1621 cgcgatcgag gtgtcggcgg cgatccggaa cgcgcagctg tacgcccggg tggaggatca
     1681 gaaccggcgc ctgcgggagc tcgacgaggc gaaggacgac ttcctgcgcg gcgtctcgca
     1741 caacctccag acgccgctcg cgagcatccg cggctatgcg cagcagctgg ccgcggacac
     1801 gcccgaccgg cgcctcggga tcatcaccga gcaggcggac cg
//
LOCUS       DBNHXO010000321         2955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_291010, whole genome shotgun sequence.
ACCESSION   DBNHXO010000321 DBNHXO010000000
VERSION     DBNHXO010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_291010"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /gene="atpD"
                     /locus_tag="AC162A_06705"
     CDS             complement(<1..1370)
                     /gene="atpD"
                     /locus_tag="AC162A_06705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR5010568.1"
                     /translation="MATAAPSANGRVIQITGPVVDIEFPAGQLPAIFNAVEIIREGEL
                     PLVCEVQQHLGNNWVRAVAMTTTDGLARGTEVRDTGAAITVPVGEVTLGRVFDVLGRP
                     IDGKGDVSAELMLPIHRAPPAFEDQTTEIEIFETGLKVIDLICPFKKGGKIGIFGGAG
                     VGKTVIIQELINNVATKHSGVSVFAGVGERSREGNDLIHEMTESGVIKSTAFVFGQMN
                     EPPGARQRVGLTGLTMAEYFRDEEGRDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGY
                     QPNLATEMAGLQERITSTRKGSITSLQAVFVPADDYTDPAPATTFAHLDSTIRLERSI
                     AELGIYPAVDPLTSTSRVLDPLVVGNDHYDVAQETKRVLQRYRDLQDIIAILGIDELS
                     EEDKATVARARRLQRFMSQPFTVAQVFTGREGKYVSIKDTVASFKEILEGKADTLPEQ
                     AFFLAGT"
     gene            complement(1400..2260)
                     /gene="atpG"
                     /locus_tag="AC162A_06710"
     CDS             complement(1400..2260)
                     /gene="atpG"
                     /locus_tag="AC162A_06710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIR5010569.1"
                     /translation="MASQREIRRRINSARNIKQITRAMQFVAASKLRRAQESTLAARP
                     YRDTLDEVIADLATVLSGDDHPLLAARDLGAEHSRLLVIITSDRGLAGALNTNTIRHV
                     AREITEHPGELKVATVGRKGRDAMRRARVPIAAHFEGFGDRPTFADVLPLARLITDDY
                     VAGAYDRVDVVYSRFVSTLVQKPVLDQLLPVEPAEDSTGIPGNQFLFEPNPADFLRAL
                     LPRYVATRLFQAVLESKASEESSRMVAMKNATENAQELIEDLTLSYNKVRQANITREM
                     VEIASGAQAR"
     gene            complement(2480..>2955)
                     /locus_tag="AC162A_06715"
     CDS             complement(2480..>2955)
                     /locus_tag="AC162A_06715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430927.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR5010570.1"
                     /translation="TDLFNAGQRPAMNIGISVSRVGSAAQTKAMKKVAGPLKLDLAQY
                     RALAAFAQFASDLDKATRDQLNRCEKLSEIVKQPQYQPLPVEKQVAILVAATSGKLDD
                     VPTPKVKEFEIGLYRFLETERPAILDELGKTKNLTDELTASLESAIDDFKLSFLA"
BASE COUNT          408 a          969 c         1082 g          496 t
ORIGIN      
        1 gtgcctgcga ggaagaacgc ctgctcgggg agcgtgtcgg ccttgccctc gaggatctcc
       61 ttgaacgagg ccaccgtgtc cttgatcgac acgtacttgc cctcgcggcc ggtgaacacc
      121 tgggcgaccg tgaacggctg gctcatgaac cgctgcaggc ggcgggcgcg ggcgaccgtg
      181 gccttgtcct cctcggacag ctcgtcgatg ccgaggatcg cgatgatgtc ctggaggtcg
      241 cggtagcgct gcaggacgcg cttcgtctcc tgggccacgt cgtagtggtc gttgccgacg
      301 acgagcgggt cgaggacgcg cgaggtcgag gtcagcgggt cgaccgcagg gtagatgccg
      361 agctcggcga tcgagcgctc gagccggatc gtcgagtcga ggtgggcgaa cgtcgtcgcc
      421 ggcgccgggt ccgtgtagtc gtcggcgggg acgaagacgg cctggagaga cgtgatcgag
      481 cccttgcggg tcgacgtgat ccgctcctgc aggccggcca tctcggtggc caggttcggc
      541 tggtagccca ccgccgacgg catccggccg aggagcgcgg agacctcgga gcccgcctgg
      601 gtgaagcgga agatgttgtc gatgaacagg aggacgtcgc ggccctcctc gtcgcggaag
      661 tactcggcca tcgtcaggcc cgtgaggccg acccgctggc gcgcacccgg gggctcgttc
      721 atctggccga acacgaaggc cgtgctcttg atgacgcccg actcggtcat ctcgtggatg
      781 aggtcgttgc cctcgcgcga ccgctcgccc acgccggcga acaccgacac gcccgagtgc
      841 ttggtcgcga cgttgttgat cagctcctgg atgatgacgg tcttgccgac gccggcgccg
      901 ccaaagatgc cgatcttgcc gcccttcttg aacgggcaga tcaggtcgat gaccttgagg
      961 ccggtctcga agatctcgat ctcggtcgtc tggtcctcga acgcgggcgg ggcgcggtgg
     1021 atcggcagca tgagctccgc gctcacgtcg cccttgccgt cgatcgggcg cccgaggacg
     1081 tcgaacacgc ggccgagcgt gacctcgccc accgggacgg tgatcgcggc gccggtgtcg
     1141 cggacctcgg tgccgcgggc gagcccgtcg gtcgtggtca tggccacggc gcggacccag
     1201 ttgttgccca ggtgctgctg gacctcgcac accaggggca gctcgccctc gcggatgatc
     1261 tcgaccgcgt tgaagatggc cggcagctgc ccggcgggga actcgatgtc gacgaccgga
     1321 ccggtgatct ggatcacccg gccgttcgcg gaaggggcgg ctgtcgccat ggtggggagt
     1381 gcctcctcgc tgcgcgggcc tagcgggcct gtgcgccgct cgcgatctcg accatctcgc
     1441 gagtgatgtt ggcctggcgg accttgttgt acgagagcgt caggtcctcg atgagctcct
     1501 gggcgttctc ggtggcgttc ttcatcgcga ccatccggga cgactcctcg ctcgccttgg
     1561 actcgagcac cgcctggaac aggcgggtcg cgacgtagcg cgggaggagc gcccgcagga
     1621 agtccgcggg gttgggctcg aacaggaact ggttgcccgg gatgcccgtg ctgtcctccg
     1681 ccggctcgac cggcagcagc tggtcgagga ccggcttctg gacgagcgtg gacacgaacc
     1741 gggagtagac gacgtcgacc cggtcgtacg cgcccgcgac gtagtcgtcg gtgatcaggc
     1801 gcgcgagcgg caggacgtcg gcgaacgtgg gccggtcgcc gaagccctcg aagtgggcgg
     1861 cgatcggcac ccgggcgcgg cgcatcgcgt cgcgaccctt gcggccgacc gtggcgacct
     1921 tgagctcgcc cggatgctcg gtgatctcgc gggccacgtg gcggatggtg ttggtgttga
     1981 gcgcgcccgc gaggccgcgg tcgctggtga tgatcacgag cagccggctg tgctccgccc
     2041 cgaggtcgcg cgcggcgagc agcgggtgat cgtcgccgga cagcacggtc gcgaggtcgg
     2101 cgatgacctc gtcgagcgtg tcccggtacg gcctagcggc cagcgtcgac tcctgcgcac
     2161 gccgcagctt ggacgccgcc acgaactgca tcgcgcgcgt gatctgcttg atattccgcg
     2221 cgctgttgat gcgccgacgg atctcacgct ggctagccac gcagggcctc ccggcggctc
     2281 gccggatcca cggtccattt cgggtggccc acccctgtgt gaagtgctgt cggtggccgt
     2341 cccgcccgtg cgcccccgcc taccaccgcg aggcgtgggc gaactgatcc gcgcgtcgtt
     2401 ccagcgactg cacgacccgc aggcgtaaac gagcccattc gactcgacga gcgtgcggcg
     2461 aggctcgggc gacgaccggt cacgccagga acgagagctt gaagtcgtcg atggccgact
     2521 cgagcgaggc ggtgagctcg tcggtcaggt tcttggtctt gcccagctcg tcgaggatcg
     2581 ccgggcgctc ggtctcgagg aaccggtaga gcccgatctc gaactccttg accttgggcg
     2641 tcgggacgtc gtcgagcttg ccgctggtcg cggcgaccag gatcgccacc tgcttctcga
     2701 cgggcagcgg ctggtactgc ggctgcttga cgatctcgga cagcttctcg cagcggttca
     2761 gctgatcgcg ggtcgccttg tcgaggtcgc tcgcgaactg cgcgaacgcc gccagcgcgc
     2821 ggtactgggc gaggtccagc ttgagcggcc cggcgacctt cttcatcgcc ttggtctgcg
     2881 cggcggagcc gacgcgcgag accgagatgc cgatgttcat cgcgggccgc tggccggcgt
     2941 tgaacaggtc ggtct
//
LOCUS       DBNHXO010000322         2284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2669485, whole genome shotgun sequence.
ACCESSION   DBNHXO010000322 DBNHXO010000000
VERSION     DBNHXO010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2669485"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..>2284)
                     /locus_tag="AC162A_06720"
     CDS             complement(152..>2284)
                     /locus_tag="AC162A_06720"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5010571.1"
                     /translation="IRLKFLPFLFLGVILRFATEAALLYGVNIVEELRVPLFGLAYGF
                     LLFTLWQNRSYPGLALAFVGVASNALVIMVNGGHMPVWMPAYEASGLTAPLGTALHVP
                     LPATLGPEFLLHLGPLGDLIPLPIPPLQNVASIGDLFLTAGLSFFLFSTVLTIPKETR
                     DAIDAAKAGRMLGVSGTAAMPSGAATARTGTLEPTISTTTATVEDAAGWSLFPPGEAV
                     SDSALRAGTGLAPGLEEASVLERPLILGGRGVGMAAPATSSSSQAMALDLAATAVGGD
                     VAIPAERPTRLEDVRQHPYVRLALNGSFSALWVGQVISLFGDRINQLALVAFVFEITD
                     SPVAVAITFIVSTIPNLIFSPVAGALVDRWDHKQVLVVSDILRAALVLLIPVAVLINV
                     WLAYPLVFAITTVSIFFRPARVAILPQIVDEEDLLSANSAMWVGETIADVINYPIAGL
                     FVVFLASSLPLAFWFDAVTYLGSAVLLATIVVPPMARRVRGTAEAVEPVDAEDETGKR
                     SIRRDLRVGWDFLRHETVLLANTLQGTVGQFAVGVFTVSSIVLAREITKAPGDEYRAT
                     YAFMETAIGLGNLVGGFALGLIATRVRKGRMIIAAYVAFGALVFLVGNTSSVYVVLGL
                     LFGVGVANMAFIIPSQTLFQERTPPELMGRVVSFRFAVVFGGMSIAMAVGGLLTSSFG
                     PGPVIAVAGLISMAAGLGGLLVKALREA"
BASE COUNT          397 a          789 c          815 g          283 t
ORIGIN      
        1 cgcgtcgtcg gcgccgggct cggctgccgg gtcgcggtcc tgatcggtca tccggtgatc
       61 ggtctcctct ccgcgggcgt cgcctcgcag gcgcggcctc gcgcggccgc cagtgtaccg
      121 accgacgagc ccgtccggcc ccgcggcccg ctcacgcctc gcgcaacgcc ttgacgagca
      181 gcccgcccag cccggccgcc atcgagatca ggcccgcgac cgcgatgacg ggaccgggac
      241 cgaagctcga ggtcagcagg ccgcccacgg ccatcgcgat cgacatcccg ccgaacacga
      301 ccgcgaaccg gaagctgacg acgcggccca tcagctctgg cggcgtccgt tcctggaaca
      361 gcgtctggct ggggatgatg aacgccatgt tcgcgacgcc gacgccgaac agcaggccga
      421 gcacgacgta caccgacgac gtgttgccga cgaggaacac gagggccccg aaggcgacgt
      481 acgccgcgat gatcatgcgg cccttgcgga cccgtgtcgc gatcaggccc agcgcgaagc
      541 cgccgaccag gttgccgagg ccgatcgccg tctccatgaa cgcgtacgtc gcgcggtact
      601 cgtcgcccgg tgccttggtg atctcccgcg ccaggacgat gctcgacacg gtgaacaccc
      661 ccaccgcgaa ctggccgacc gttccctgga gggtgttcgc caggagcacc gtctcgtggc
      721 gcaggaagtc ccagccgacg cgcaggtcac gtcggatcga ccgcttgccc gtctcgtctt
      781 cggcatcgac aggctccacc gcctccgccg tcccgcgcac ccgccgtgcc atcggcggca
      841 cgacgatggt cgccagcagc accgccgagc cgaggtaggt caccgcgtcg aaccagaacg
      901 cgaggggcag cgacgaggcg aggaagacga cgaacaggcc cgcaatcggg tagttgatga
      961 cgtcggcgat ggtctcgccg acccacatcg cggagttcgc cgagaggaga tcctcctcgt
     1021 cgacgatttg cggcaggatc gcgacccgcg ccgggcggaa gaagatcgag acggtcgtga
     1081 tcgcgaacac cagcgggtac gcgagccaga cgttgatcag cacggcgacc gggatcaaca
     1141 ggaccagcgc cgcgcgcagg atgtccgaga cgaccaggac ctgcttgtgg tcccagcgat
     1201 cgacgagggc gcccgccacg ggcgagaaga tcaggttcgg gatcgtggac acgatgaacg
     1261 tgatcgcgac ggcgacgggg gagtccgtga tctcgaacac gaaggcgacg agcgcgagct
     1321 ggttgatccg gtcgccgaac aggctgatca cctggccgac ccagagggcc gagaacgagc
     1381 cgttgagcgc gagacggacg tatgggtgct ggcggacgtc ctcgagccgc gtggggcgtt
     1441 ccgcggggat ggcaacgtcg cccccgaccg ccgtggcggc caggtccagg gccatcgcct
     1501 ggctcgacga gctggtggca ggcgccgcca tgcccacgcc gcgaccaccc aggatcagcg
     1561 gccgttccag gaccgaggct tcctcgaggc ccggcgcgag gccggtgccg gcccgcaggg
     1621 ccgaatccga gaccgcctcg cccggcggga agagggacca gccggcggcg tcctcgacgg
     1681 tcgccgtcgt cgtgctgatc gtcggctcga gcgtcccggt tcgggcggtg gccgctcccg
     1741 aaggcatggc tgccgtcccg gacacgccga gcattcgccc ggccttcgcc gcgtcgatcg
     1801 cgtcccgcgt ctccttgggg atcgtgagca cggtcgagaa caggaagaac gacaggccgg
     1861 cggtgaggaa caggtcgccg atcgaggcga cgttctggag cggtgggatg ggcagcggga
     1921 tcaggtcacc gagcgggccc aggtgcagga ggaactcggg cccgagggtc gcgggcagcg
     1981 gcacgtgcag cgcggtgccc aggggagcgg tgaggccgga cgcctcgtac gccggcatcc
     2041 agaccggcat gtggccaccg ttgaccatga tcacgagcgc gttgctcgcc accccgacga
     2101 acgccagcgc caagcctgga tacgaccggt tctgccacag cgtgaacagc aggaagccgt
     2161 aggccaggcc gaacagcggc acgcgcagct cttcgacgat gttgacgccg tagagcagtg
     2221 ccgcctcggt cgcgaagcgc aggatcacgc cgaggaacag gaacggcagg aacttgaggc
     2281 ggat
//
LOCUS       DBNHXO010000323        13250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1481281, whole genome shotgun sequence.
ACCESSION   DBNHXO010000323 DBNHXO010000000
VERSION     DBNHXO010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 13250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13250
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1481281"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="AC162A_06725"
     CDS             complement(<1..913)
                     /locus_tag="AC162A_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012356.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR5010572.1"
                     /translation="MRYVLPVGGTDPGRLRLGDEVLFRVRAGPDAPVDRVFLRTAPDG
                     EQVLSELAEATPGAACRWFEVTARLEAPVTDYRFLVVGASGYHWLNGRGLYREDVTDR
                     DDFRLVAGFDPPSWLADRVFYQVFPDRFANGDPTNDVGDGSWIYRGHPSRRRAWGDRP
                     TDGPGGSVDFFGGDLAGLEARLDHLVGLGVNAIYLNPIFESRSNHGYDTIDYGRVAAH
                     LGGDDALVSLRRATEARDIRLILDIAPNHTGAEHPWFLEAQADPDAPTAGYYTFQRRP
                     DEYESWLGVRSLPKLDYRDHGLREAMYA"
     gene            complement(976..1827)
                     /locus_tag="AC162A_06730"
     CDS             complement(976..1827)
                     /locus_tag="AC162A_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014909673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIR5010573.1"
                     /translation="MTGQNWFAAVGWRHLVAIGAIIFALIPIAFVVSAAFNPLGTLSS
                     TDLIPRQFGTANFENLFSKTDFARWFVNSILIAGCATFFSLALATFAAFAFSRYRFSG
                     RRPGLLFLLIVQMFPAFLAIVTIYIIFTKITDLYPIIGFNTPWSLVLLYLGGAMGVNT
                     WLIKGFFDTVPRELDESAKVDGASHTTTFFQIILPLVVPILVVVGLLGFIGAINEFLM
                     ASVFLTQGDSKTLAVGLYGLIAGERNANFGMFAAGTLLTAIPTVVLFYGLQRYIVGGL
                     TSGAVKG"
     gene            complement(1824..3395)
                     /locus_tag="AC162A_06735"
     CDS             complement(1824..3395)
                     /locus_tag="AC162A_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017557050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5010574.1"
                     /translation="MTAISTTPRFGLAAGSTRGLLGKILLLAIVAAIAVAAAIPLVNG
                     EEWFWLTVLVLVTVAIFFIYLQPWHIPLKYIVPGTIFLVAFQVIPVVSTFGVSFTNFG
                     DGHRGTKDDAITAVEGSSVKQVPGSAEYVLTIATLGDVATGNLQFLLYDPTTQTVQVG
                     AADGLTPCTDCTTGPTGKVTAAGDLTLLNLGQAAARSADVQAFSVPTDNGAIRASGVS
                     KAYEGLATATYDPACDCITDSTTGTVYTADNTRGSFINAETATALPQGWQVNVGLSNY
                     TRAFTDSSVAGPFLGILGWNFAFAVLTVLTTFVAGLLVAIALNSPRLRALRFYRLLIV
                     LPYAMPSFAMLLVWRDMFNTDFGLINNLTGQSVNWFGGVWTARFAVLLVQFWLGYPYM
                     FLVCLGALQSIPADMTEAAAVDGAKPYQAFRAVVFPLLLVAVAPLLIASFAFNFNNFN
                     VIQLTTGGAPFPPDNVKAGGTDLLVSYTYRLAFTEGGAQYGFAAAISVLIFVIVAVFS
                     IIGFRRTAALEEINR"
     gene            complement(3526..4833)
                     /locus_tag="AC162A_06740"
     CDS             complement(3526..4833)
                     /locus_tag="AC162A_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014693350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR5010575.1"
                     /translation="MIKRMGVLLAMMVIVVGACTSGGSGATTAPSAAATSAASAAAPS
                     EGAATPAASADAGAAKLLIWADDKRAAAIKPLAQQWATDNGVTVEVQAITENLTTQFK
                     TASQQGTGPDIVVWAHDAVADFVQNSAIDPVTMPDTSMFDPLAVKGMTYQGQLYGVPY
                     SIENIALIRNTDLAPECPATMEELVETGQQLVKDKKVTNIMALQVGQKGDAYHIYPLF
                     TSGGGSFFGTTATGDPDGSNVTVDSPESIAAGQKLFDLGEKGVGALKRSVDDKNAIPL
                     FTGGKTPFLVSGPWAIADIVKAKVPYDVCAIPMFEGGKPASPFIGVNGFYLASNGKNK
                     TLAQEFLTSVVPTADFQSGLYAVDPRRPAMTEAATAAAAADPNIPKFAEAGANGTILP
                     AIPEMGQVWSPFGIAESAIVGGTDPSTALSAAAKAIRDGIANQ"
     gene            complement(4898..6037)
                     /locus_tag="AC162A_06745"
     CDS             complement(4898..6037)
                     /locus_tag="AC162A_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR5010576.1"
                     /translation="MEERTGRRPRIADVAREAGVSKTAVSFAFNRPSRLAPETAIRIR
                     TVADSLGYRPDPVARMLTQRSTRTLGVLTPQALSVIFSNPFFGAFSEGVALAAEQEGF
                     ALDFISPLHGSLTRAIERAMVDGVVAIGVSAQHGEVEQLRVAGLPLVMVDSSALPDLP
                     TIEVDDEAGAFAAAQHLLALGHRDILVVGIESTPGTPPEPDGVGGRRARGYRKALAGA
                     GIELMRGAYVVAPSTIEGGMAALRRTWEDGLRPTGVLVMSDVMAIGVLRAARELGLRV
                     PADLSVVGFDDIDISQHTHPPLTTVHQPIRLKGETAGRLVLAMIEGRDPRPASVRLDT
                     RLIIRESTGPVARSAKRTDATCRPVRLQRTSGHPNPNPRGGQPTA"
     gene            6268..6639
                     /locus_tag="AC162A_06750"
     CDS             6268..6639
                     /locus_tag="AC162A_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010577.1"
                     /translation="MLDQLRAMPPGIRIFLGYAFLILGAIGLSLRWVVDQAISVPISP
                     IGVVAMVLLAYTIFTTTLVIQRKEASRTLALGLATLTVPAVPLLLLAAQPLFAVFIAV
                     LSALLFFGLTRPEVRTWLNEP"
     gene            6776..7690
                     /locus_tag="AC162A_06755"
     CDS             6776..7690
                     /locus_tag="AC162A_06755"
                     /inference="COORDINATES: protein motif:HMM:NF022747.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HIR5010578.1"
                     /translation="MTEQRQPSTPSGSKPAPATPSTRRPSTSPTGSRRVGRRETTRRR
                     YDEPSFIERYRTVIMVVVVVAVVGLLGAFVFTSATATAFACTTIDTVQPSASGELGQV
                     QPDMGQTHVGRGDKVTYPVCPPASGKHVNAPPYGPIPAKFYGPDDTAIPQGWIHNLEH
                     GGLVLLYSCDKGACDAAAQEQLQAFYANFPSSPICGVPPGSISPVVARFEQMPTRYAA
                     MVWDRVLYLDELDSAKVLDFFNRYAERLDSEGNWLAPPEPQCAAPSPSPSAEESPSAE
                     ASPSSAASPSASTPPSPAVEPQPSPSPS"
     gene            7702..8679
                     /locus_tag="AC162A_06760"
     CDS             7702..8679
                     /locus_tag="AC162A_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR5010579.1"
                     /translation="MRLYGYEDRFRDTRFGVLVDDRLLTGSQLEKRGGLRMDTHLGLG
                     LHIQFDPGWEGSIQKATRRALRNRAPLIDPESRRPVAVIDQVGLGGKVVCVGLNYADH
                     VKEGGRTAPDRPMLFAKFANSIVGDGDAIVRPPGTRALDLEVELGVVIGRTARRVTED
                     DALDHVAGYVVVNDVSARDWQGNVAALREGEKGDGQWLRAKGSDTFLPVGPVFVTRDE
                     IDPAAGLGLRSWRIPGSGPDAGRALPMQAGSTADMVFGVAALVSFISQQITLEPGDLI
                     ATGTPAGVGVFRDPPVFLEPGDRVRCEIEGIGVVENPVVDWTDDPRPGA"
     gene            8728..9783
                     /gene="tdh"
                     /locus_tag="AC162A_06765"
     CDS             8728..9783
                     /gene="tdh"
                     /locus_tag="AC162A_06765"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HIR5010580.1"
                     /translation="MLALVKTAPGPGLSLEDVPMPQIGINDVLIRVHRTGICGTDLHI
                     ETWDAWAAKTIQPPLITGHEFVGEIVETGSNVYDFAPGEIVSGEGHVTCGLCRHCMAG
                     RRHLCAHMVGLGVGRDGAFAEYVALPMTNIWHHWPGIDEDVAAIFDPFGNAVHTALAF
                     PVLGEDVLITGAGPIGLMATAVARHAGARYIVVSEPNPVRRELALRMGATVAVDPRER
                     DLGDVGRELGMVEGYDVALEMSGNPAALRGALDTMAHGGSIAILGIPTEDISLDVNTI
                     VFKLLTIRGIYGREMYETWYKMSVMVQSGLDITPAITHRFGFREHEAAFAAARSGDAG
                     KVILDWVGRDAPEEAHR"
     gene            9780..11006
                     /locus_tag="AC162A_06770"
     CDS             9780..11006
                     /locus_tag="AC162A_06770"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009676059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HIR5010581.1"
                     /translation="MTGTVRPNPLGYLDDELEAMRERHLYRPLRVMSSAQGPIVSVDE
                     RRVISLSSNDYLGLSHHPRMKRAAIEAVERFGAGSGAVRTIAGTMTLHEALETELAAF
                     KHTEAVLTFQSGFTANTGVIPTITGETDLIVSDELNHASIIDGMRLSKAPRKVFPHAD
                     VDALREILRTAREGGRDGHGEPHRLILVVTDGVFSMDGDIAPLPGIVEAAEAYGAAVM
                     VDDAHASGVLGKDGRGSVSHFGLEGRVAVQVGTLSKAVGVLGGYVAGSLALREVLIQR
                     ARPFLFSTSHPPAVAAACSEAIRIMVEEPELIERLWTNTRRFKAELGRLGFDIGRSET
                     PITPVILGDVETTIRFSGRLFEEGVFGTSVVFPTVALDRSRIRTIVTAAHTDEMLDQA
                     LAAFDRVGRELGVISG"
     gene            11003..11941
                     /locus_tag="AC162A_06775"
     CDS             11003..11941
                     /locus_tag="AC162A_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HIR5010582.1"
                     /translation="MTRRQPAEEAADTRPRAEVEREVFLAGIAAGLGEGRDLPLDAHL
                     HTVRSPDADAQIEAYCALAVERGIAEIAITDHVDFDSTMPAYGFATFADRERDVRGAA
                     ERWADRGLAVRFGVEVTYERRYEDEIRGWLQRHPHDFVIGSVHISARSPYKAGRVAGF
                     AAGRSLPDIVEPYFDEVLAAVRSGLFDTLGHLDFVKRYLVPHVLPADLAAAPELYEPI
                     LAALVDGGTALEVNASGLRQLPRETYPSAAIVARYHELGGRAVTVGSDAHRADWFAYG
                     LRQAYRQAASSGFEALTFRRGADRVLVPMPVGADGG"
     gene            12034..12789
                     /locus_tag="AC162A_06780"
     CDS             12034..12789
                     /locus_tag="AC162A_06780"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5010583.1"
                     /translation="MRLTVLGAGPAYTDRPEAAGASYLVSSGTTNVLLDLGHGSFSRI
                     FAAVAPEDLAAVAISHLHPDHFVDLVPFRHYLRYYLHPSRRMRVIGPGDLATRLDALH
                     AEPGFASQALDIEPIGSSDHAVGDLVVRARKIAHTDDSYGMRVSREPDASTGAGLVYS
                     GDCGRADDLRPLIAPGDTLLTEVSFGPGPVPAGVLHLDGPAVGALATATGVGRVLLTH
                     LQMGFDPGATLASVRTAYDGPVEFVWPGSQIEI"
     gene            complement(13059..>13250)
                     /locus_tag="AC162A_06785"
     CDS             complement(13059..>13250)
                     /locus_tag="AC162A_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010584.1"
                     /translation="ETPVGSVDAETGTPNEDVTPPSTDTLTPTNTPSSDGWQLVLLAL
                     AAMLATALILTPASRKARR"
BASE COUNT         1988 a         4593 c         4709 g         1960 t
ORIGIN      
        1 ccgcgtacat cgcctcgcgc aggccgtggt cgcggtagtc gagcttgggc agcgatcgga
       61 cgcccagcca cgactcgtac tcgtcgggcc ggcgctggaa cgtgtagtag ccggccgtcg
      121 gggcatcggg atcggcctgc gcctcgagga accaggggtg ttcggcgccc gtgtggttgg
      181 gggcgatgtc gaggatgagc cggatgtcgc gtgcttccgt cgcgcggcgc agcgagacca
      241 gcgcgtcgtc gccgccgagg tgcgccgcga cgcgcccgta gtcgatggtg tcgtagccgt
      301 ggttcgaccg cgattcgaag atcgggttga ggtagatcgc gttgacgccg agtccgacga
      361 ggtggtcgag gcgcgcctcg agccccgcga gatcgccgcc gaagaagtcg accgagccgc
      421 ccggcccgtc ggtcgggcgg tcgccccacg cgcgccggcg acttggatgc ccgcgataga
      481 tccagctccc gtccccgacg tcgttggtcg ggtcgccgtt ggcgaaccga tcgggaaaca
      541 cctggtagaa gacgcgatcg gcgagccatg acggcgggtc gaagcccgcc accagccgga
      601 agtcgtcccg atcggtcacg tcctcccgat acaggccgcg accgttgagc cagtggtacc
      661 cggacgcccc gacgaccagg aaccggtagt ccgtcaccgg cgcctccagg cgggcggtga
      721 cctcgaacca tcggcaggcc gcgccgggcg tcgcctccgc gagctccgat agcacctgct
      781 cgccgtccgg tgccgtgcgc aggaacacgc ggtcgaccgg ggcgtcgggc ccggcgcgca
      841 cgcggaacag gacctcgtcg ccgaggcgca gccggccggg gtccgttccc ccgaccggca
      901 acacgtagcg caccgagccg tcgtgatgga ccgattcgag accgtgggtc gtcgaaccgg
      961 tccctgggcg cccgatcaac ccttgaccgc accggaggtg aggccgccga cgatgtagcg
     1021 ctggagtccg tagaacagga cgaccgtcgg gatcgccgtc agcagcgtcc cggccgcgaa
     1081 catcccgaag ttcgcgttgc gctcgccggc gatcaggccg tacagaccca cggcgagcgt
     1141 cttgctgtcg ccctgggtca ggaacacgct ggccatcagg aactcgttga tcgccccgat
     1201 gaaccccagc aggccgacga cgacgaggat cggcacgacc aggggcagga tgatctggaa
     1261 gaacgtcgtg gtgtgcgagg cgccgtcgac cttcgcggac tcatcgagct cccgcggaac
     1321 ggtgtcgaag aagcccttga tcagccacgt gttgacgccc atcgcgccgc ccaggtagag
     1381 cagcaccagg ctccacgggg tgttgaagcc gatgatcggg tagaggtcgg tgatcttggt
     1441 gaagatgatg tagatcgtca cgatggccag gaacgcgggg aacatctgga cgatcagcag
     1501 gaacagcagt cccgggcgac ggcccgagaa gcgatagcgc gagaaggcga aggcggcgaa
     1561 cgtcgccagc gcgagcgaga agaacgtcgc gcagcccgcg atcaggatcg agttgacgaa
     1621 ccaccgcgcg aagtccgtct tcgagaacag gttctcgaag ttcgcggtgc cgaactggcg
     1681 cgggatcagg tcggtcgagc tcagcgttcc caacgggttg aacgccgccg agacgacgaa
     1741 ggcgatcggg atcagcgcga agatgatcgc gccgatcgcc accagatggc gccagccgac
     1801 ggcggcgaac cagttctgac cggtcatcgg ttgatctcct cgagggcggc ggtgcgccgg
     1861 aacccgatga tcgagaacac ggcaacgatc acgaagatca gcacggagat ggcggcggcg
     1921 aagccgtact gggcgccgcc ctcggtgaac gcgagccggt acgtgtagct caccagcagg
     1981 tccgtgccgc ctgccttcac gttgtcaggt gggaacgggg caccgcccgt cgtcagctgg
     2041 atgacgttga agttgttgaa gttgaacgca aacgaggcga tcagcagcgg cgccaccgcg
     2101 accaggagca gcgggaacac gaccgccctg aatgcctggt acggcttggc gccgtcgacc
     2161 gcggctgcct cggtcatgtc cgccgggatg gactggaggg cgcccaggca gaccaggaac
     2221 atgtacgggt agccgagcca gaactggacg agcagcacgg cgaaccgcgc ggtccacacg
     2281 cccccgaacc agttgaccga ctggccggtc aggttgttga tgagcccgaa gtcggtgttg
     2341 aacatgtcac gccataccag cagcatcgcg aacgagggca tggcgtacgg caggacgatg
     2401 agcagccggt agaaccgcag cgcccggagc cgcggtgaat tgagcgcgat cgccaccagc
     2461 aggccggcca cgaaggtcgt gaggacggtg aggacagcga acgcgaagtt ccagccgagg
     2521 attccgagga acggcccggc aacggaggag tcggtgaacg cccgggtgta gttgctgagg
     2581 ccgacgttga cctgccagcc ctgcggcagt gccgtggcgg tctcagcgtt gatgaagctg
     2641 ccccgcgtgt tgtccgccgt atagaccgtc cccgtggtcg agtcggtgat gcagtcgcac
     2701 gccgggtcgt aggtcgcggt ggccaggccc tcgtatgcct tgctcacccc cgatgcccgg
     2761 atcgcgccgt tgtcggtggg aacgctgaac gcctggacgt ccgccgaccg tgccgctgcc
     2821 tggccgaggt tcagcagcgt caggtcgccc gcggccgtga ccttgccggt tggcccggtc
     2881 gtgcagtcgg tgcacggcgt caggccgtcc gcggcgccca cctggaccgt ctgggtggtt
     2941 gggtcgtaga gcaggaactg gaggttcccg gtcgcgacgt cgcccaacgt cgcgatcgtc
     3001 aggacgtact ccgcggagcc cggtacctgc ttgaccgacg atccctcgac cgccgtgatc
     3061 gcgtcgtcct tcgtgccgcg atggccgtca ccgaagttcg tgaacgacac gccgaacgtg
     3121 gacacgaccg ggatgacctg aaaggcgacc aggaagatcg tgccgggcac gatgtacttg
     3181 agtgggatgt gccacggctg cagatagatg aagaagatgg cgacggtcac caggaccagc
     3241 accgtgagcc agaaccactc ctcgccgttg acgagcggga tggcggcggc aacagcgatc
     3301 gccgcaacga tcgcaagcag caggatcttc ccgagcagcc cccgggtgct gcccgcggcc
     3361 agcccgaacc tcggggtggt tgatatcgca gtcaccggat cgctcctcct ccgtcacccc
     3421 gtggggcgcc agcgggcgcc gtgaaccgtg tcatcggccg gcggccggcg gatgcggccg
     3481 gtgcgaccct cacggatcgc ccggccgcat cgcgcgcgtc gtcgactact ggttggcgat
     3541 cccgtcgcgg atcgccttgg cggcggccga gagcgccgtc gacgggtcgg tcccgcccac
     3601 gatcgccgac tcggcgatgc cgaatgggct ccagacctgc cccatctcag ggatcgcggg
     3661 caggatcgtg ccgttggctc cggcctccgc gaacttcggg atgttcgggt cagcggcggc
     3721 cgccgcggtg gcggcctcgg tcatcgccgg gcggcgcggg tcgaccgcgt acaggcccga
     3781 ctggaagtcg gcggtcggga cgacgctcgt gaggaactcc tgggcgagcg tcttgttctt
     3841 gccgttgctg gcgaggtaga agccattgac gccgatgaac ggcgacgcgg gcttgccacc
     3901 ctcgaacatc gggatggcgc agacgtcgta ggggaccttg gccttcacga tgtccgcgat
     3961 ggcccacggg ccggagacga ggaagggggt cttgccgccg gtgaacagcg ggatggcgtt
     4021 cttgtcatcg acggaacgct tgagcgcacc gacgcccttc tcgcccaggt cgaacagctt
     4081 ctggccggcg gcgatggact ccggggagtc gacggtgacg ttggacccat ccgggtcacc
     4141 ggtcgccgtg gtgccgaaga acgaaccgcc ccccgacgtg aacagcgggt agatgtggta
     4201 ggcgtcgccc ttctggccga cctggagggc catgatgttg gtgaccttct tgtccttgac
     4261 cagctgctgg ccggtctcga ccaattcctc catcgtggcc gggcactcgg gcgcgaggtc
     4321 cgtgttccgg atcagggcga tgttctcgat cgaatacggc acgccgtaga gctggccctg
     4381 gtaggtcatg cccttgacgg ccagcggatc gaacatcgac gtgtcgggca tcgtgaccgg
     4441 gtcgatggcg ctgttctgga cgaagtcagc caccgcgtca tgcgcccaga cgacgatgtc
     4501 ggggcccgtc ccctgctggc tggcggtctt gaactgtgtc gtcaggttct cggtgatcgc
     4561 ctgaacctcg acagtcactc cgttgtcggt ggcccactgc tgggcgagcg gcttgatcgc
     4621 ggcggcgcgc ttgtcgtccg cccagatcag gagcttcgcg gcgccggcgt cagccgacgc
     4681 ggccggggtg gcagcgccct ctgacggcgc tgcagcggat gcggccgagg ttgcggccgc
     4741 gctaggcgcg gtggtcgcgc cggacccgcc gctggtacac gcgccgacga cgatcaccat
     4801 catcgccagc agaacaccca ttcgcttgat cacgttgatt caacctcctc cattgccgtg
     4861 tggacgtgtc tcgtcagcct tcgtgcatcg actcctttca tgcggtcggt tggccgcctc
     4921 gtggattcgg attcgggtgc cccgacgttc gttgcaatcg gacaggccgg catgtcgcgt
     4981 cggtccgctt cgcggagcgg gcgacagggc cggtggactc gcggatgatc aggcgcgtat
     5041 cgaggcggac cgatgcgggt cgggggtcac gaccttcgat catcgccagc acgagccggc
     5101 cggcggtctc gcctttcagc cggattggct ggtggaccgt cgtcagtggc gggtgggtgt
     5161 gctggctgat gtcgatgtcg tcgaagccga cgacggacag gtcggcgggc acgcgcaggc
     5221 cgagctcgcg agcggcgcgc aggacgccga tcgccatcac gtcggacatc acgagcacgc
     5281 cagtcgggcg caggccgtcc tcccaggtcc gacggagcgc cgccatcccg ccctcgatgg
     5341 tggacggggc gacgacgtat gcgccacgca tcagctcgat ccccgcgccg gcgagcgcct
     5401 tccgatagcc gcgcgcccgg cggccgccga ccccgtccgg ctccggcggc gtgcccggtg
     5461 tcgactcgat acccacgacg agaatgtcgc ggtggccgag cgccagcagg tgctgggccg
     5521 ccgcgaacgc gcccgcctcg tcgtcgacct cgatcgtcgg caggtcgggc agcgcgctcg
     5581 aatcgaccat caccaacggc agcccggcga cccgcagctg ctcaacctcg ccatgctgcg
     5641 cggacacgcc gatcgcgacc acgccatcga ccatcgcccg ctcgatcgca cgggtcagcg
     5701 acccgtggag cggcgagatg aagtcgagcg cgaagccctc ctgctctgcg gccagcgcga
     5761 cgccctccga gaacgccccg aagaacgggt tggagaagat caccgacagc gcctggggcg
     5821 tgagcacgcc gagggtccgc gtcgatcgct gggtgagcat ccgggcgacc gggtcgggcc
     5881 gatagcccag ggagtccgcg accgtgcgga tccggatcgc ggtctcgggt gccagccggc
     5941 tgggccggtt gaaggcgaac gagaccgcgg tcttcgagac gcccgcctcg cgggcgacgt
     6001 cggcgatgcg cggacggcgc ccggtccgct cctccacggc cacctcgggt gtggcgaccg
     6061 gattgccggc tgacggcggg tggtccgatc cccgggacct gccgtggttg ccgtgtgcgg
     6121 gcgccagatc gctccgtcaa ccggtttagc aaaccggttt agcgcggaat ctacagggat
     6181 tccgaggggt gtcaaggacg attcgaaacg gtctcgatcc ggcgctcggc accccgttgc
     6241 gtggcccgat ggcatgatgc cgaggacatg ctcgaccagc tgcgcgcgat gccccccggc
     6301 atccggatct tcctgggcta cgccttcctg atcctgggcg cgatcgggct gtccctgcgc
     6361 tgggtggtcg accaggcgat cagcgtgccg atctcgccga tcggcgtggt ggcgatggtc
     6421 ctgctcgcgt acacgatctt cacgacgacc ctcgtgatcc agcgcaagga ggcgtcgcgc
     6481 acgctcgccc tcgggctggc gacgctcacc gtgccggcgg tcccactcct gctgctcgcg
     6541 gcacagccgc tgttcgcggt gttcatcgcc gtcctgtcgg cgctcctgtt cttcgggctg
     6601 acgcgacccg aggtacgcac ctggctgaac gagccctgag gccgcccgcc ggtggtgacc
     6661 ggcggcgcaa ctccgacggg gacgctaccc ttcggccagc gcaccgcggc tgcggccggg
     6721 gagccacgac gaccggtcgt cgcgaccgtc gctgcagcac gtggagcacc gcaccttgac
     6781 cgaacagaga caaccgtcga cgcccagcgg gtccaaaccc gcgccggcga caccgtcgac
     6841 ccggagaccg tcgaccagcc cgaccgggag ccgtcgcgtc ggccggcgcg agaccacacg
     6901 ccgccggtac gacgagccgt cgttcatcga gcgataccgg acggtgatca tggtggtcgt
     6961 ggtggtcgcc gtcgtgggcc tcctgggcgc gttcgtgttc acctcggcga ccgcaacggc
     7021 cttcgcgtgc acgaccatcg acacggtcca gccgtccgcc tcgggcgagc tcggccaggt
     7081 gcagcccgac atgggccaga cgcatgtcgg ccgcggcgac aaggtgacct acccggtctg
     7141 cccacccgcg tccggaaagc acgtcaacgc gccgccctac ggcccgatcc ccgccaagtt
     7201 ctacgggcct gacgacaccg ccatccccca aggctggatc cacaacctcg agcatggcgg
     7261 cctcgtcctc ctctactcgt gcgacaaggg cgcctgcgat gcggcagcgc aggagcagct
     7321 ccaggcgttc tacgcgaact tcccgagcag cccaatctgc ggcgtgccgc cgggctcgat
     7381 cagcccggtt gtcgcacggt tcgagcagat gccgacgagg tacgcggcca tggtgtggga
     7441 ccgcgtcctg tacctcgacg agctcgacag cgcgaaggtc ctcgacttct tcaaccgcta
     7501 cgcggagcgg ctcgacagcg agggcaactg gctggccccg cccgagcccc agtgtgcggc
     7561 gccctcgccc agcccctccg ccgaagagag cccgtcggcc gaggcgagtc cgtcgtcagc
     7621 cgccagcccg agcgcgtcga ccccgccctc gccggccgtc gagccgcagc cttcgccgtc
     7681 gccgagctga gcccaggggc catgcggctg tacgggtacg aggaccgatt cagggatacc
     7741 cgattcggcg tcctcgtcga cgaccggctg ctgactggct cgcagctcga gaagcgtggc
     7801 ggcctccgca tggacacgca tctcggcctc ggactgcaca tccagttcga cccaggctgg
     7861 gaagggtcga ttcagaaggc gacccgccgg gcgctgcgca accgcgctcc gctgatcgat
     7921 cccgagtccc gtcgaccggt cgcggtcatc gaccaggtcg gcctgggcgg caaggtcgtg
     7981 tgcgtcgggc tcaactacgc cgaccacgtg aaggagggcg gccgcacggc gccggaccgg
     8041 ccgatgctgt tcgcgaagtt cgccaattcg atcgtcggcg acggcgacgc gatcgttcgt
     8101 cccccgggca ctcgcgccct cgatctcgag gtcgagctgg gggtcgtcat cggtcggacg
     8161 gcgcggcggg tcaccgagga cgacgccctg gaccacgtcg ccggctacgt cgtcgtcaac
     8221 gacgtgagcg cgcgcgactg gcagggcaac gtcgccgcgc tgcgcgaggg cgagaagggt
     8281 gacggccagt ggctgcgggc aaagggcagc gacacgttcc tgccggtcgg cccggtcttc
     8341 gtcacgcgcg acgagatcga tcccgccgcc ggcctgggcc tccgctcgtg gcggatcccc
     8401 ggcagcggtc cggacgccgg ccgagccctc ccgatgcagg ccggttcgac cgccgacatg
     8461 gtgttcggcg tcgccgccct cgtcagcttc atctcccagc agatcacgct cgagcccggc
     8521 gacctgatcg cgaccggcac gcccgccggc gtcggcgtgt tccgggaccc gcccgtcttc
     8581 ctcgagcccg gtgaccgcgt ccgatgcgag atcgagggca tcggcgtggt ggagaatccg
     8641 gtcgtcgact ggaccgacga cccgcgccca ggcgcctgac agcgccacgg accggatgac
     8701 cccggagcca gtgcagcagg agcgccgatg ctcgctctcg tgaagaccgc tcccgggccc
     8761 gggctcagcc tggaagacgt gccgatgccg cagatcggca tcaacgacgt gctgatccgt
     8821 gtccaccgca cgggcatctg cggcaccgac ctgcacatcg agacctggga cgcctgggcc
     8881 gcgaagacga tccagccgcc gctcatcacg gggcacgagt tcgtcggcga gatcgtggag
     8941 accggctcca acgtctacga cttcgcaccc ggcgagatcg tgagcggcga aggccacgtc
     9001 acctgtggcc tgtgccgcca ctgcatggcg ggccgccgcc acctgtgcgc ccacatggtc
     9061 ggcctgggcg tgggacgcga cggtgcgttc gccgaatacg tcgcgctgcc gatgaccaac
     9121 atctggcacc actggccggg catcgacgag gacgtggcgg cgatcttcga cccgttcggc
     9181 aacgccgtgc acacggcgct cgcattcccg gtgttgggcg aggacgtgct gatcaccggg
     9241 gcggggccga tcggactgat ggcaaccgcg gtcgcgcgcc atgcgggcgc ccgctacatc
     9301 gtcgtgagcg agcccaaccc cgtccggcgc gagctggcgc tgcggatggg ggcaaccgtc
     9361 gccgtcgacc cccgcgagcg cgacctcggc gacgtcgggc gggagctggg gatggtcgag
     9421 ggctacgacg tcgccctcga gatgtcgggc aacccggcgg ccctccgtgg cgcgctcgac
     9481 acgatggccc acggcggcag catcgcgatc ctgggcatcc cgaccgagga catctcgctc
     9541 gacgtcaaca cgatcgtgtt caagctgctg acgatccggg ggatctacgg ccgggagatg
     9601 tacgagacct ggtacaagat gtcggtgatg gtccagtcgg ggctggacat cacgcccgcg
     9661 atcacccacc ggttcgggtt ccgcgagcac gaggctgcgt tcgcggcggc ccgctcgggc
     9721 gacgcgggca aggtcatcct ggactgggtc ggccgcgacg cacccgagga ggcccaccga
     9781 tgaccggcac cgtccgcccg aacccgctcg gctacctcga cgacgagctc gaggcgatgc
     9841 gcgagcgcca cctctatcgg ccgctgcggg tgatgtcgtc cgcccagggc ccgatcgtct
     9901 cggtcgacga gcggcgggtc atcagcctgt cctcgaacga ctatctcggg ctgtcgcatc
     9961 acccgcggat gaagcgggcg gcgatcgagg cggtcgagcg attcggcgcg ggatcggggg
    10021 ccgtgcggac gatcgccggc accatgacgc tgcacgaggc gctcgagacg gagctcgccg
    10081 cgttcaagca caccgaggcc gtcctcacgt tccagtcggg cttcacggcc aacaccggcg
    10141 tgatcccgac gatcaccggc gagaccgacc tgatcgtgtc cgacgagctc aaccatgcct
    10201 cgatcatcga cggcatgcgg ctctcgaagg cgccgcgcaa ggtgttcccc cacgcggacg
    10261 tcgatgcgct gcgcgagatc ctgcgcacgg cccgcgaggg cgggcgcgac ggccacggcg
    10321 agccgcaccg gctcatcctc gtcgtcaccg atggcgtgtt cagcatggac ggcgacatcg
    10381 cgccgctgcc cggcatcgtc gaggcggccg aggcctacgg cgccgcggtg atggtcgacg
    10441 acgcgcacgc gtcgggcgtg ctgggcaagg acggccgggg ctccgtgagc catttcgggc
    10501 tcgaggggcg ggtcgcggtc caggtcggca cgctgtccaa ggccgttggc gtgctcggcg
    10561 ggtacgtcgc ggggtcgctg gccctgcgcg aggtgctgat ccagcgcgcg cggccgttcc
    10621 tgttctccac ctcgcatccg cccgcggtcg ccgcggcctg tagcgaggcg atccggatca
    10681 tggtcgagga gcccgagctc atcgagcgcc tgtggacgaa cacccgccgg ttcaaggcgg
    10741 agctcggtcg cctgggcttc gacatcgggc gctcggagac gccgatcacc ccggtgatcc
    10801 tgggcgacgt cgagaccacg atccggttct cgggtcgcct gttcgaggag ggcgtgttcg
    10861 ggacgtcggt cgtgttcccg acggtcgcgc tcgatcgctc ccggatccgc acgatcgtga
    10921 ccgcggcgca caccgacgag atgctggacc aggcgctcgc cgcgttcgat cgcgtcggcc
    10981 gtgagctggg cgtgatcagc gggtgacccg gcgccaacca gccgaggagg ccgccgacac
    11041 ccggccccgg gctgaggtcg agcgcgaggt cttcctcgcc ggtatcgcgg ccgggctggg
    11101 cgagggtcgg gacctgccgc tcgacgcgca tctccacacg gtgcggtcgc cggacgcgga
    11161 tgcgcagatc gaggcctact gcgcgctcgc cgtcgagcgg gggatcgccg agatcgcgat
    11221 caccgaccac gtggacttcg actccacgat gcccgcctac gggttcgcga ccttcgcgga
    11281 ccgggagcgg gacgtccgcg gggccgccga gcgctgggcc gatcgcggcc tggccgtccg
    11341 gttcggggtc gaggtgacct acgagcggcg ctacgaggac gagatccggg gctggctgca
    11401 gcgacacccc cacgacttcg tcatcggcag cgtccacatc agcgcccggt cgccgtacaa
    11461 ggcgggcagg gtcgccgggt ttgccgcggg gcgctccctg cccgacatcg tcgagcccta
    11521 cttcgacgag gtcctcgcgg ccgtccggtc cggcctgttc gacacgctcg gccacctcga
    11581 cttcgtcaag cgctacctcg tcccgcacgt cctgcccgcc gacctcgccg ccgcacccga
    11641 gctgtacgag ccgatccttg cggcgctggt cgacggcggc accgcactcg aggtcaacgc
    11701 ttcggggctg cgccagctgc cccgcgagac gtacccctcg gcggcgatcg tggcccgcta
    11761 ccacgagctc ggcgggcgag cggtgacggt cggatcggat gcccatcgcg cggactggtt
    11821 cgcctacggg ttgcggcagg cctatcgcca ggcggcctcg tcggggttcg aagccctgac
    11881 cttccgacgc ggcgcggatc gcgtgctcgt gccgatgccc gtcggggccg acgggggctg
    11941 aagtcagggc tggcgtcgcg gccctcggcg gcggattctc cgagcacgcg catgcgtccg
    12001 ccgctacgct cgcggagtga cgacgaacga cgcgtgcgcc tgacggtcct cggcgccggg
    12061 ccggcgtaca cggaccgccc cgaggccgcc ggcgccagct acctggtctc gtccggcacc
    12121 accaacgtcc tgctcgacct gggccatggg tcgttctcga ggattttcgc ggcggtcgct
    12181 cccgaggacc tggccgcggt ggcgatcagc cacctccatc ccgaccactt cgtcgatctc
    12241 gtcccgttcc ggcactacct gcgctactac ctgcacccct cgcggcggat gcgcgtgatc
    12301 ggacccggcg acctcgcgac acggctcgat gcattgcacg ccgagcccgg gttcgcgagc
    12361 caggcactgg acatcgagcc gatcgggtcg tcagaccacg cggtcggtga cctggtcgtc
    12421 cgcgcccgca agatcgccca cacggacgac agctatggca tgcgggtctc gcgcgaaccg
    12481 gacgcgtcca ccggcgccgg cctcgtctac agcggggact gcggtcgcgc cgacgacctc
    12541 cgcccgctca tcgcgccggg cgacacgctg ctgaccgagg tgtcgttcgg acccggaccg
    12601 gtgccggccg gcgtgcttca cctcgacggc ccggccgtcg gcgcgctcgc caccgctacc
    12661 ggcgtcggac gggtgctgct gacacacctg cagatgggct tcgatcccgg ggcgaccctc
    12721 gcgtcggtcc gcaccgcgta tgacggcccc gtcgagttcg tctggccggg gtcgcaaatc
    12781 gagatctgag gcccggcccg ctcgcagcgg tgccggaacg ccatcggggc cggtgcgacc
    12841 cggatgggtc tgaggtacgg cgacagaccg ctccgaagcg ctcctgcccg gacccgaggg
    12901 cgatcccgag agaccgcgcg gctgcctggg tcctggtacg accgtccggg atcgggcacc
    12961 ggcgcgcgtt gtcggttcgc accgtgcggg agcggcccgg acagcgaaag accggggcca
    13021 ggtggccccg gtctcgtgcg tcggctcgga gatggtccct agcggcgggc cttgcggctg
    13081 gccggggtca ggatcagcgc ggtggcgagc atggcggcca gggcgaggag caccagctgc
    13141 cagccgtcgc tcgacggggt gttggtcggg gtgagggtgt cggtcgacgg cggggtgacg
    13201 tcctcgttgg gcgtcccggt ctcggcgtcg acgctgccga cgggggtctc
//
LOCUS       DBNHXO010000324         1921 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4258230, whole genome shotgun sequence.
ACCESSION   DBNHXO010000324 DBNHXO010000000
VERSION     DBNHXO010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4258230"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..784
                     /locus_tag="AC162A_06790"
     CDS             26..784
                     /locus_tag="AC162A_06790"
                     /inference="COORDINATES: protein motif:HMM:NF015648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR5010585.1"
                     /translation="MSYADGLLANDETVVRREHQHWIFPFYIAGKWVAVAVLVTVVGF
                     ILSQWVFKSDGTGIIGGTIAFINNVVTIVTVIALAFAVIGFIYSVIQWQSQEYILTSH
                     RVLHVHGVINKQSGDSSLENVTDAQIRVPWLGRILGFGDLVVMTASEAGINNLRALRD
                     PIGFKKAMMESKTERMIVLNTPRATPQAPAAPAPVPPPVAPAPAPAPVAPVPPPPDAD
                     EVAKTIASLATLRDSGAITPEEYEAKKQELLDRI"
     gene            834..908
                     /locus_tag="AC162A_06795"
     tRNA            834..908
                     /locus_tag="AC162A_06795"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:866..868,aa:Met,seq:cat)
     gene            complement(1110..>1921)
                     /locus_tag="AC162A_06800"
     CDS             complement(1110..>1921)
                     /locus_tag="AC162A_06800"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR5010586.1"
                     /translation="DLRRAIERREFVLHYQPVVDLTAGTILGFEALVRWEHPTRGLLS
                     PASFVPAAEATGLIVWIGAWVLEEACQQAQRWQTSIPIVPPLSMSANLSARELREPGL
                     VENVAAILKRTGVDPATIVIEITETSMVEDADGAIATLQELKALGVRLAIDDFGTGYS
                     SLSYLRRLPVDVLKLDRSFVAPAGRGDRESALVDAVFRLGRSLGLVTVAEGIEDVEQR
                     DRLVSLGCRVGQGYLFARPMAEPEATEYLLAHRAPAGDAAPGDGRNRGAAA"
BASE COUNT          289 a          687 c          655 g          290 t
ORIGIN      
        1 gccgcgggcg cgagggagat cgaccatgtc atatgccgac gggctcctgg cgaacgacga
       61 gaccgtcgtc cggcgggagc accagcactg gatctttccg ttctacatcg cgggcaagtg
      121 ggtcgccgtc gcggtcctcg tgaccgtcgt cggattcatc ctgtcgcagt gggtgttcaa
      181 gtcggacggg accgggatca tcggcggcac gatcgccttc atcaacaacg tggtcacgat
      241 cgtcacggtg atcgccctcg cgttcgcggt gatcggcttc atctactcgg tgatccaatg
      301 gcagagtcag gagtacatcc tcaccagcca tcgcgtgctc cacgtccacg gcgtgatcaa
      361 caagcagtcc ggtgacagct cgctcgagaa cgtcaccgat gcgcagatcc gggtgccctg
      421 gctggggcgg atcctgggct tcggcgatct cgtcgtcatg accgcatccg aggccggcat
      481 caacaacctg cgcgccctgc gggacccgat cgggttcaag aaggccatga tggagtcgaa
      541 gaccgagcgg atgatcgtcc tcaacacgcc gcgcgcgacg ccccaggcgc ccgccgcccc
      601 cgctcctgtc ccgccgccgg tcgccccggc cccggccccc gcgccggtcg cccccgtgcc
      661 gccgccgcca gatgccgacg aggtcgcgaa gacgatcgcg tcgctggcca ccctgcgcga
      721 ctcgggcgcc atcacccccg aggagtacga ggcgaagaag caggagctgc tcgaccgcat
      781 ctagggcacg ccggcctttg acggaaccgc cgatcgccgg tagtgttgat agccgcgggg
      841 tggagcagcg gcagctcgtc gggctcataa cccgaaggtc aggggttcga atcccctccc
      901 cgcaaccaat tcgcttcgcc tgaaccccgg gacccgacca ccccggggtt cttctttgcc
      961 ctttcgccgt tgatccggct cagcccggcg ttggctcctg cgcgcgctcc ctcacgcatc
     1021 atcagatgcg ctcggtcacg tgctcgtcgc cgccttggtc tgagcgccgc tgagcggcga
     1081 tctactgcgc ggcaccgctg gatccggacc taggcggcgg cgccgcggtt gcggccgtcg
     1141 cccggagcgg cgtcgccggc cggagcccgg tgggccagca ggtactccgt cgcctcgggc
     1201 tctgccatcg ggcgggcgaa caggtagccc tggccgacgc ggcagccgag gctgaccagg
     1261 cggtcccgct gctccacgtc ctcgatgccc tcggcgacgg tcacgaggcc cagcgaccga
     1321 ccgagacgga acaccgcgtc cacgagggcc gactcgcggt cgccgcggcc cgccggcgcc
     1381 acgaacgagc ggtcgagctt gagcacgtcg acagggaggc ggcgcaggta gctgagcgac
     1441 gagtagcccg tgccgaagtc gtcgatggcc aggcggaccc cgagcgcctt gagctcctgc
     1501 agcgtcgcga tcgctccgtc ggcgtcctcg accatgctgg tctcggtgat ctcgatcacg
     1561 atcgtcgctg ggtcgacgcc cgtgcgcttg aggatcgccg cgacgttctc gaccaggccg
     1621 ggctcgcgca gctcgcgcgc cgagagattg gcgctcatcg agagcggggg cacgatcgga
     1681 atgctcgtct gccagcgctg ggcctgctgg cacgcttcct cgagcaccca ggcgccgatc
     1741 cagacgatga ggccggtcgc ctcggcggcg ggcacgaacg acgccggcga gaggagccca
     1801 cgggtcgggt gctcccagcg gacgagcgcc tcgaagccga ggatcgtgcc tgcggtcagg
     1861 tcgaccacgg gctggtagtg gaggacgaac tcgcgccgct cgatcgctcg ccgcaggtcc
     1921 g
//
LOCUS       DBNHXO010000325         2671 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2274770, whole genome shotgun sequence.
ACCESSION   DBNHXO010000325 DBNHXO010000000
VERSION     DBNHXO010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2274770"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="AC162A_06805"
     CDS             <1..891
                     /locus_tag="AC162A_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014926.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HIR5010587.1"
                     /translation="AWAGAGYEIGWAQVPLDDAEADGTGAEGAGTPHQGWTGGVAVDD
                     EGYLLHPSFAAPPALSLWRAPTDNDRIGGMAGRWDAWGLASLTRRLEAVERGDDAVTV
                     RATWTTAAGIEVAHAQRLSSSPDGRVRVEETVWIPSVVEDLPRVGTVLELTTGHEAFE
                     WFGRGPHETYPDRRRGGRVGLWRSTVTDQLVPYVRPQENGGHADTRWFRVRAPRGGIR
                     VDLDRPGQVSATHVTAADLDTATHDVELVPRPETIVHLDAAHRGLGTASCGPDTLEPY
                     VLRGGTYQWAWTLGTEDAAT"
     gene            888..>2671
                     /locus_tag="AC162A_06810"
     CDS             888..>2671
                     /locus_tag="AC162A_06810"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HIR5010588.1"
                     /translation="MTVDWDERARQWHLHNGRTSWAMHVLENDWLGHLHAGPPIRPGA
                     SLRHLGPPAAFTSFSNRVGEPVGLEVPVPGIGDFRMPALSVDLPDGSAVLDLRYAGHR
                     VAAGKPGLPELPSTYAEADAEAETLEIDLVDAPTGLRVTVRTTLFADRPVVARSLTIT
                     NGGEAPMTIRCAMSAVLDLPDSDWNLVTLSGAWARERHVDDRPLVTGRQSVGSLRGAS
                     SLYASPFLALRRPSTTEDAGEAFGMSLVYSGNFLAEAEVEPFGTTRLRIGIHPEAFAW
                     RLEPGGTFTTPEAVIAWSGDGLGGLSEALHGLYRERLARGRWRDRPRPVVLNNWEATY
                     FDFNHERIVAIAGKAKDLGVELFVLDDGWFGRRDNDDSSLGDWVVDRRKLPGGIEALA
                     RDVRALGLTFGLWIEPEMVNADSDLFRTHPDWAIGVPGRSRTESRQQLVLDFARREVV
                     DHIADALIAVLASAPIDYVKWDMNRNITEPWTAAVPSDRQGEFFHRYILGVYDLYRRL
                     TERFPDILFESCSSGGGRFDAGMLAYAPQAWTSDDTDAVERLPIQWGTSLAFPVSSMA
                     AHVSAVPNHQTGRLAPLFTRAAVAMFGA"
BASE COUNT          358 a          985 c          968 g          360 t
ORIGIN      
        1 gcgtgggcgg gtgccggcta cgagatcggc tgggcgcagg tgcccctgga cgatgcggag
       61 gcggacggga cgggcgcgga gggcgccggc acaccacatc agggctggac cggcggcgtc
      121 gcggtcgacg acgaggggta tctcctccac ccctcgttcg cggcgccgcc ggccctgtcc
      181 ctctggcggg cgccgacgga caatgaccgg atcggcggca tggccggccg ctgggacgcc
      241 tggggcctgg cgtcactgac ccggcggctc gaggccgtcg agcggggcga cgatgccgtc
      301 acggtgcgag cgacctggac gacggccgcg ggaatcgagg tcgcccacgc gcagcggctg
      361 tcgagctcgc ccgacggacg ggtccgggtc gaggagacgg tctggatccc gtccgtcgtc
      421 gaagacctgc cgcgcgtggg cacggtcctc gagctcacca ccggccacga ggccttcgag
      481 tggttcggcc gcgggcccca cgagacgtat cccgaccgtc gccgcggcgg acgcgtcgga
      541 ctgtggcggt cgaccgtgac cgaccagctc gtcccgtacg tccggccgca ggagaacggc
      601 ggtcacgccg acacgcgctg gttccgggtc cgggcgccca ggggcggcat ccgcgtggac
      661 ctcgaccggc ccgggcaggt gtcggcgacg cacgtgacgg ccgccgacct cgacacggcg
      721 acccacgacg tcgagctcgt gccgcgcccc gagaccatcg tccacctcga cgccgcccac
      781 cgtggcctcg ggaccgcgag ctgcgggccg gacacgctcg agccctacgt gctgcgcggc
      841 gggacgtacc aatgggcctg gacgctgggc acggaagacg ccgccacgtg accgtcgact
      901 gggacgagcg ggcgcgccag tggcacctcc acaacggccg caccagctgg gcgatgcacg
      961 tcctggagaa cgactggctg gggcacctgc acgccggccc gccgatccgc cccggcgcat
     1021 cgctgcggca cctgggaccg ccggccgcgt tcacgagctt ctcgaaccgc gtcggcgagc
     1081 ccgtcggcct cgaggtcccg gtcccgggca tcggcgactt ccggatgccc gccctgagcg
     1141 tggatctccc cgatggatcg gccgtgctcg acctgcgcta cgccggacac cgggtcgccg
     1201 ccggcaagcc ggggctgccc gagctgccgt cgacctacgc ggaggcggac gccgaggccg
     1261 agacgctcga gatcgacctc gtcgacgccc cgacgggcct gcgggtcacc gtccggacga
     1321 cgctgttcgc cgatcgaccg gtcgtcgccc gctccctcac catcaccaac ggtggcgagg
     1381 cgccgatgac gatccggtgc gcgatgtcgg ccgtcctcga cctcccggac tcggactgga
     1441 atctcgtgac cctcagcggc gcctgggcgc gggagcggca cgtcgacgac cggccgctgg
     1501 tgacgggccg gcagtccgtc ggcagcctgc gcggcgcctc cagcctctac gccagcccgt
     1561 tcctcgcgct gcgccgcccg tcgacgaccg aggacgccgg cgaggcgttc ggcatgagcc
     1621 tcgtgtactc gggcaacttc ctcgccgagg ccgaggtcga accgttcggg acgacccggc
     1681 tgcggatcgg catccatccc gaggcgttcg cgtggcgcct cgagcccggc gggacgttca
     1741 ccacgcccga ggccgtgatc gcctggtctg gtgacggcct gggcgggttg agcgaggcgc
     1801 ttcacgggct gtaccgcgag cggcttgcac gcggccggtg gcgcgatcgc ccccgcccgg
     1861 tcgtgctcaa caactgggag gcgacgtact tcgacttcaa ccacgagcgc atcgtcgcca
     1921 tcgccggcaa ggcgaaggac ctcggggtcg agctgttcgt gctcgatgac gggtggttcg
     1981 ggcggcgcga caacgacgac tcgtcgctgg gcgactgggt ggtcgaccgg cgcaagctcc
     2041 cgggcggcat cgaggcgctg gcccgtgacg ttcgagccct cggcctcacg tttgggctgt
     2101 ggatcgagcc cgagatggtc aacgccgaca gcgacctgtt ccgcacccat cccgactggg
     2161 cgatcggcgt gcccggccgc tcgaggaccg agagccgcca gcagctcgtg ctcgacttcg
     2221 cgcgccgcga ggtcgtcgac cacatcgccg acgcgctgat cgcggtcctc gcgagcgccc
     2281 cgatcgacta cgtcaagtgg gacatgaacc gcaacatcac cgagccctgg acggcggcgg
     2341 tgccgtcaga ccgccagggc gagttcttcc accgctacat cctgggcgtc tacgacctct
     2401 accggcggct caccgagcgc ttccccgaca tcctgttcga gtcgtgctcc agcggcggcg
     2461 ggcggttcga cgccgggatg ctggcgtacg cgccgcaggc gtggaccagc gacgacacgg
     2521 acgcggtcga acggctgccg atccagtggg gaacctcgct cgccttcccg gtgtcgtcga
     2581 tggcggccca cgtgtccgcg gtgcccaacc accagaccgg gcgcctcgcg ccgttgttca
     2641 cgcgcgcggc ggtcgcgatg ttcggtgcct t
//
LOCUS       DBNHXO010000326         4140 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2275131, whole genome shotgun sequence.
ACCESSION   DBNHXO010000326 DBNHXO010000000
VERSION     DBNHXO010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2275131"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..877)
                     /locus_tag="AC162A_06815"
     CDS             complement(59..877)
                     /locus_tag="AC162A_06815"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR5010589.1"
                     /translation="MTGVAAALPVISRAERFTRVGSTNDIVRGWLAAGEPEVCLAVAD
                     EQTAGRGRHGRTWTAPPGSALLASLGFRPSWLAPDHAWRLAACVALAMCDAAEAVAAL
                     PTGAVRLKWPNDLVIEVAGPDAIELRKLAGVLGESEGLGSDDPRVVVGIGINADWAAD
                     DFPAELAGSMTSLQEASGGLAIDRDALLAAFTERLEGRVEALRGGAFDIATWAGRQAT
                     TGRWVTIDDDPIAVRALGVDAASGGLLVADTGDPARERIVHAGEVRRVRLAPAV"
     gene            complement(882..2186)
                     /locus_tag="AC162A_06820"
     CDS             complement(882..2186)
                     /locus_tag="AC162A_06820"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR5010590.1"
                     /translation="MPGPLVVMKFGGSSVAGAERIKRVARRIVRERAGGSQLVVVVSA
                     MGDTTDELLSLAAAITDEPDARELDVLLATGEHQSATLVSMALHSLGVPAISLTGPQA
                     GITTDGRHGRARIAGIDPRRVRAELAAGKVVIVAGFQGMSVAASEGLGDTSTPSDVHA
                     AEITTLGRGGSDTTAVALAARLHADRCQIYTDVRGIYTADPRIESGARQLSVIGYEEM
                     MELAHQGAQVMQTRAVELGWVNDVVIEVLSSFEDAPGTQIREDPLVEQRNKVRGLAHD
                     RNVAKITLVEVPDKPGVARSVFEPLAEAGINVDMIVQNVGHGGATDLSFTIPRVELAR
                     AKRLLEPLIRDLGFRDMTTDSSVAKVSIVGAGIQNAPGYAARMFGTLADAGINIEMIS
                     TSEIRITTIIAEDRVEEAVRALHAAFELEQPETVETAGAAAG"
     gene            complement(2368..3156)
                     /locus_tag="AC162A_06825"
     CDS             complement(2368..3156)
                     /locus_tag="AC162A_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5010591.1"
                     /translation="MDLNRFGRAIRALRRRRHWRQSDLADAAHVSASQVGRVERGESR
                     RLPVESLERVALALGASLDLTIRWKGEGLDRLLDEAHARLVELVVRNLRAMGWDVAVE
                     VSFSIRGERGSIDVLAFHPGSRLLVVIEVKSVTPDMQAMLFGLDRKGRLAPAIARDRG
                     WFPTVTVRLLVLADTRTNRRRLDAHAATVRAALPAGTREVLRWLRDPRVQPRPGVWFV
                     SDRRGMDANDRARERVAVRRASSPTKHVQPAVSSPRDGPSSRTR"
     gene            3300..4064
                     /locus_tag="AC162A_06830"
     CDS             3300..4064
                     /locus_tag="AC162A_06830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR5010592.1"
                     /translation="MCFDHDSRPPIPPIEGGSLDARGVALDAADGNHFAAYLARAAEP
                     TGAGIVVLPDVRGLHAYYRDLALRFAENGIDAIAIDYFGRTAGIGDRGTGFEFQEHVP
                     LTTYDGLRADVTAAAAYLRAETGTTRLFAIGFCMGGRLSFLSAGFGEGLAGVIGFYGW
                     PVGPARNGTPAPADVAATFQSPVLAIFGGGDEGIGQPAISTFETALAAAGVRHEVTVY
                     PKAPHSFFDRKADEYSTTSAKAWEQTLGFIRAGGAA"
BASE COUNT          566 a         1539 c         1470 g          565 t
ORIGIN      
        1 gacgacgcct cctcgcgccg ctccctcccg ccttccaaac accggtcgcg ccatcctgtt
       61 acaccgccgg cgcgaggcgg acgcggcgga cctcgcccgc atgcacgatc cgctcgcggg
      121 ccgggtcgcc cgtgtccgcg accaggagcc cgcccgaggc cgcgtccacg ccgagggcac
      181 ggaccgcaat cgggtcgtcg tcgatcgtca cccagcggcc ggtcgttgcc tggcgcccgg
      241 cccaggtggc gatgtcgaag gcgccgcctc gcagggcttc gacccggcct tcgagccgct
      301 ccgtgaacgc cgccaagagc gcgtcccggt cgatcgccag ccctccggat gcctcctgca
      361 ggctggtcat cgagcccgcc agctcggccg ggaagtcgtc cgcggcccag tcggcgttga
      421 tgccgatgcc gacgaccacg cgcggatcgt cgctgccgag cccctcgctc tcgcccagca
      481 cgccggcaag cttgcgcagc tcgatggcgt ccggtccggc gacctcgatc acgaggtcgt
      541 tcggccactt gaggcggacg gccccggtgg ggagcgcggc gacggcctcg gccgcgtcgc
      601 acatcgcgag cgcgacgcac gccgccaggc gccaggcgtg gtccggcgcg agccagctcg
      661 ggcggaagcc caaggaggcc agcagcgcgg atcccggcgg cgcggtccag gtgcggccgt
      721 gccgcccgcg gcccgccgtc tgctcgtcgg cgaccgccag gcagacctcg ggctcgccgg
      781 cggcgagcca gccacggacg atgtcgttgg tcgagccgac ccgcgtgaag cgttcggcac
      841 ggctgatcac gggcagcgcg gcggcgacac cggtcacgtc gtcagccggc ggcggcgccg
      901 gccgtctcga cggtctcggg ctgctccagc tcgaacgcgg cgtgcagcgc gcgaacggcc
      961 tcctcgacgc gatcctcggc gatgatcgtc gtgatccgga tctccgacgt cgagatcatc
     1021 tcgatgttga tgcccgcgtc cgcgagggtg ccgaacatcc gcgcggcgta gcccggcgcg
     1081 ttctggatgc cggcgccgac gatcgagacc ttggccacgg agctgtcggt cgtcatgtcg
     1141 cggaagccga ggtcccggat caggggctcg agcagccgct tggcgcgcgc gagctcgacc
     1201 cgcgggatcg tgaacgagag gtccgtcgcc cccccgtggc caacgttctg gacgatcatg
     1261 tcgacgttga tcccggcctc ggccaggggc tcgaagacgc tccgggcgac gcccggctta
     1321 tcgggcacct cgacgagcgt gatcttggcg acgttccggt cgtgggcgag gcccctgacc
     1381 ttgttccgct gctccacgag gggatcctcc ctgatctggg tgccgggcgc gtcctcgaac
     1441 gagctgagga cctcgatcac gacgtcgttg acccagccga gctcgacggc gcgggtctgc
     1501 atcacctggg cgccctggtg ggcgagctcc atcatttcct cgtacccgat gacgctgagc
     1561 tggcgggcgc ccgactcgat ccggggatca gccgtgtaga tcccgcggac atcggtgtag
     1621 atctggcagc gatcggcatg gaggcgggcc gcgagcgcca ccgccgtcgt gtccgagccg
     1681 ccccggccga gcgtggtgat ctcggccgcg tggacgtcgc tcggggtaga ggtgtcgccg
     1741 aggccctcgc tcgcggccac gctcatgccc tggaagccag cgacgatcac gaccttgccg
     1801 gcggcgagct cggcgcgaac acgccggggg tcgatgccgg cgatccgtgc ccgcccgtgg
     1861 cgaccgtcgg tggtgatccc ggcctggggg ccggtcaggc tgatcgccgg gacgccgagc
     1921 gagtgcaggg ccatcgagac gagtgtggcg ctctggtgct cgccggtggc caggaggacg
     1981 tccagctcgc gggcgtcggg ctcgtcggtg atggccgcgg cgagggacag gagctcgtcg
     2041 gtcgtgtcgc ccatcgccga cacgacgacc acgagctgcg acccgcccgc gcgctcgcgc
     2101 acgatccgcc gcgcgacccg cttgatgcgc tccgcgcccg cgacggacga gccgccgaac
     2161 ttcatcacga cgaggggtcc gggcatgcgc ggcattctag cggagggcct tcggcgaact
     2221 cccccgccgg gccgcccctc ccgatccgtc agatcgccgc cccgtgaacg tcgtgaccga
     2281 gcgccaggac gtctgcgcgt ccttcgtgcc cagggtgagg cggccccctg cgctctgatc
     2341 gtcagatcgc cgccaaacgg cggtacgtca gcgagttcgt gacgaagggc catcccgagg
     2401 cgaactgaca gcaggctgca cgtgtttcgt aggcgacgac gcacgccgca ccgcgacccg
     2461 ttcgcgagcc cggtcgttcg cgtccatccc gcggcgatct gacacgaacc agacgccggg
     2521 gcgcggctgg acccgcgggt cacgcagcca ccgcagcacc tcccgcgtcc ccgccgggag
     2581 cgccgcccgg acggtcgccg cgtgtgcgtc gaggcggcgc cgattggtcc gcgtgtccgc
     2641 gaggacgagc agccgcacag tgactgtcgg gaaccagcca cggtcgcggg cgatcgcagg
     2701 cgcgaggcgg cccttgcggt ccaggccgaa cagcatcgcc tgcatgtccg gcgtgaccga
     2761 cttgacctcg atgaccacca gcagccggga accggggtgg aaagcgagca cgtcgatgga
     2821 tccgcgctcg cctcggatgg agaatgagac ttcgacggcc acgtcccagc ccatggcgcg
     2881 aaggttccgg acgactagct cgacgagccg tgcgtgcgcc tcgtccagca gccggtcgag
     2941 accctcgccc ttccagcgaa tcgtgaggtc gagcgaggcg ccgagggcca gggcgacccg
     3001 ctccaggctt tcgaccggca ggcgacggct ctcgcctcgc tcgacacggc cgacctgcga
     3061 ggcggagacg tgcgcggcat cggcgaggtc cgattggcgc cagtgccgac gtcgtcgcaa
     3121 cgcccggatg gcccgcccga agcggttgag atccatgccc gagcaggatg gacgcggcaa
     3181 cttatcgcgc ggttaccgcc ggcctgtccg ccatcgatgc ggcgcaatgg catccggcaa
     3241 gcgacaatcg acacgcgaac cgccccacgc tgcggtccga cggacggagg cccaccgcga
     3301 tgtgcttcga ccacgactcc cggccgccca tcccgccgat cgaaggtggt tcgctcgacg
     3361 cccgcggggt cgccctcgac gccgccgacg gcaaccactt cgccgcctac ctcgcccgcg
     3421 cggccgagcc gaccggcgcc ggcatcgtcg tcctgcccga cgtccgcggc ctccacgcct
     3481 actaccgaga cctcgcgctc cggttcgccg agaacggcat cgacgcgatc gccatcgact
     3541 acttcggccg cacggccggc atcggcgacc gcgggaccgg attcgagttc caggagcacg
     3601 tcccgctcac gacctacgac ggccttcgcg cggatgtgac cgcggccgcc gcctacctcc
     3661 gcgccgagac gggtacgacc cgcctgttcg cgatcggctt ctgcatgggc ggccgcctgt
     3721 cgttcctgag cgcgggtttc ggcgaggggc tggccggggt gatcggcttc tacggctggc
     3781 cggtcgggcc tgcccgcaac gggacgccgg cgccggccga tgtcgccgcg acgttccaga
     3841 gcccggtgct cgcgatcttc ggcggtggcg acgagggcat cgggcagccc gcgatctcga
     3901 cgttcgagac cgcgctcgcg gcggccggcg tgcgccacga ggtgacggtc taccccaagg
     3961 cgccccacag cttcttcgac cgcaaggccg acgagtactc gacgacgtcg gccaaggcgt
     4021 gggagcagac gctcggcttc atccgtgccg ggggagccgc ctgagatgag cttcctccgg
     4081 cgcctgttcg gcggcgccgc gccggacgat cgcgggccgg ctgcggacga gcccgcgccg
//
LOCUS       DBNHXO010000327         2130 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1877294, whole genome shotgun sequence.
ACCESSION   DBNHXO010000327 DBNHXO010000000
VERSION     DBNHXO010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1877294"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /locus_tag="AC162A_06835"
     CDS             complement(<1..1365)
                     /locus_tag="AC162A_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5010593.1"
                     /translation="MSSPEPLQRPLGPPTPDPKGWSRRELDTLTAIADTFVAGDAPRR
                     ARLAADALNLAADPDQVLQLRLVMRAFDSRLANLLLTGRAVRFRSLDLAGRERYLLAW
                     ATSRIAQRRSAYHGLKRLLTFFAYADPGVDGENPRLAATGYEHRPEPVTTDPTPIQPH
                     DLAPLATTIRGDDPIVLEADVVVVGSGAGGGVVAADLARAGRSVVVLEAGPFVPEKDM
                     PQDELAAFDRLYLNHGLNVSWDASILTLVGGAVGGGTLVNWLTCVAPPEAVRHGWATR
                     HGVAGFDGPGVDADIEAIERELGVREPPNVPPKDQLIIRGATVLGHEVGETRRNGVDC
                     GDCGRCAFGCRRGAKQSGLRAHLAEAWRHGARIVPDAPVERVIVEDGTATGVDASVVL
                     DGRPRRLIVRAPAVVVAAGTLRTPVVLLRSGVGHPAMGRHLRLHPVSILAAFLDEDVT
                     MWRGT"
     gene            complement(1415..>2130)
                     /locus_tag="AC162A_06840"
     CDS             complement(1415..>2130)
                     /locus_tag="AC162A_06840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5010594.1"
                     /translation="DHFLVFATVDRAKEHKGITAFVLERGMAGLTSGTLHGKLGIRAG
                     NTGLIDMEDVRVPVEHRIGEEGEGFLIAMSAIDQGRFTVAAGAVGLAQACLDASIRYA
                     HERETFGEEIGRHQLVKQMIANMAKGTEIGRLLVFQAAVLKNRGLRNTRETSLAKWHA
                     TDHSVQAALDAIQIHGANGYSNEFPVERYLRNSKAAVIYEGTSQLHTLIQADYALGYR
                     QDRPLRCEPWPAQGFERPA"
BASE COUNT          281 a          777 c          771 g          301 t
ORIGIN      
        1 cgtcccgcgc cacatcgtga cgtcctcgtc gaggaacgcg gccaggatcg acaccgggtg
       61 gaggcgcagg tgacgaccca tggccgggtg accgaccccg gaccggagca acacgacggg
      121 cgtgcgcaac gtgccggccg cgaccacgac cgccggcgcc cggacgatga gccgccgcgg
      181 tcgtccgtcg agcacaacgc tcgcatcgac gcccgtcgcg gtcccgtcct cgacgatgac
      241 ccgttcgacc ggggcgtccg ggacgatccg tgctccatgc cgccacgctt cggcgaggtg
      301 ggcccgcagc ccggactgct tggcgccgcg ccggcagccg aaggcgcacc ggccgcagtc
      361 cccgcagtcg acgccgttgc gacgcgtctc cccgacctcg tgccccagca cggtcgcgcc
      421 gcggatgatc agctggtcct tgggcggcac gttcggcggt tcccggacgc cgagctcgcg
      481 ctcgatggcc tcgatgtcgg cgtccacccc cggcccgtcg aacccggcga cgccgtggcg
      541 cgtcgcccag ccgtggcgga ctgcctcggg aggcgcgaca caggtcagcc agttgacgag
      601 cgtcccgccg cccaccgccc cgccgacgag cgtgaggatt gaggcgtccc aggagacgtt
      661 gagcccgtgg ttgaggtaca gccgatcgaa ggcggccagc tcgtcctggg gcatgtcctt
      721 ctccgggacg aacggccccg cctccagcac gaccaccgaa cggccagcgc gagcgaggtc
      781 ggccgcgacc acgccgccgc cggcgcccga ccccacgacc acgacgtccg cctcgagcac
      841 gatcgggtca tcgccgcgaa tggttgtcgc gagcggcgcg aggtcgtggg gctggatcgg
      901 ggtcggatcg gtcgtgaccg gctccggacg gtgctcgtag ccggtcgccg ccagccgcgg
      961 gttctcgccg tccacgcccg ggtccgcgta ggcgaagaac gtcagcaggc gcttgaggcc
     1021 gtggtaggcg gaccgccgct gggcgatgcg cgaggtcgcc cacgccagca ggtaccgctc
     1081 gcgaccggcg aggtcgaggc tccggaagcg cacggcgcgc cccgtcagca gcaggttcgc
     1141 gaggcgcgag tcgaacgctc gcatcacgag ccgcagctgg agcacctggt ccgggtccgc
     1201 ggcgaggttc agcgcgtcgg cggcgagcct cgcccgccgc ggggcgtccc ccgcgacgaa
     1261 cgtgtctgcg atggccgtga gcgtgtcgag ctcgcgccgc gaccagccct tcgggtccgg
     1321 agtgggtggc cccagtggac gctgcagcgg ctcggggctg gacatctccg cctgatggta
     1381 ggcgctgggc aggagcgccg tcggtctccc ggcctcaggc cgggcgctcg aagccctgcg
     1441 cgggccacgg ctcgcagcgc agcggccggt cctggcggta gccgagcgca tagtcggcct
     1501 ggatcagggt gtgcagctgg ctggtgccct cgtagatcac ggcggccttg ctgttgcgca
     1561 ggtagcgctc gaccgggaac tcgttggaat agccgttggc gccgtggatc tggatcgcgt
     1621 cgagggcggc ctgcaccgag tggtcggtcg cgtgccactt ggcgagggag gtctcgcggg
     1681 tgttgcgcag gcccctgttc ttgagcacag ccgcctggaa caccagcagc cgcccgatct
     1741 cggtcccctt ggccatgttg gcgatcatct gcttgaccag ctggtggcgg ccgatctcct
     1801 cgccgaaggt ctcgcgttcg tgcgcgtagc ggatcgatgc gtcgaggcac gcctgggcga
     1861 ggccgaccgc acccgccgcc accgtgaacc ggccctggtc gatcgcgctc atcgcgatca
     1921 ggaagccctc cccctcctcg ccgatccggt gctcgaccgg gacgcgcacg tcctccatgt
     1981 cgatgaggcc ggtgttgccg gcccggatcc ccagcttgcc gtggagcgtg ccgctcgtga
     2041 ggcccgccat cccccgttcg agcacgaacg ccgtgatgcc cttgtgctcc ttcgcccggt
     2101 cgaccgtggc gaacacgagg aagtggtcag
//
LOCUS       DBNHXO010000328         2754 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_886541, whole genome shotgun sequence.
ACCESSION   DBNHXO010000328 DBNHXO010000000
VERSION     DBNHXO010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_886541"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="AC162A_06845"
     CDS             <1..180
                     /locus_tag="AC162A_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010595.1"
                     /translation="TAADLARLGSDGTTPDDLVRRSFEFLLAREPKSSILARFDLPLI
                     GRYFPEYEASITRPR"
     gene            complement(164..1348)
                     /locus_tag="AC162A_06850"
     CDS             complement(164..1348)
                     /locus_tag="AC162A_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5010596.1"
                     /translation="MADTLGRTDRMVASVEHVFSWFRNGSWERHHLDPGIADFTFGNP
                     QELPLPGLVEALQRNAVPRDKDWFAYKMSEEEPRQVVVESLRRRTGLPYAPEDVAMTA
                     GAFGALAVTIRALCDVGDEVIYLSPPWFFYELMIASSGASAVRVDLATPTFDLDPDAV
                     AAAITPRTRAIIVNSPHNPTGRIYREPELRALGDVLRAASERNGRTITLISDESYNRI
                     VFDGIAFRSPAVDYDATALIYTYGKTTLAPGQRIGFVALHPSFPDKQTVGYRIFVQQL
                     AAGWGFPNALLQHSIRDLEGLSVDIAVLQARRDRMVPALREMGYEVTRPEGTFYLMVH
                     SPDPDDLAFTDRLAELGALVLPGTIVESPGWFRVSLTASDAMVERGLEAFRAAAPAIA
                     GA"
     gene            1750..2478
                     /locus_tag="AC162A_06855"
     CDS             1750..2478
                     /locus_tag="AC162A_06855"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HIR5010597.1"
                     /translation="MCEQFVARAAEPFRLDELWPFAERLERYGLAGFGWGATWLRAGG
                     GLETHRHTTAFCDDPARARIGAIETTSLLVHLRRPSRLSTLDLPDTQPFDDPAGRFAF
                     SHNGDLRDYRAARERYRLQGRIFGRADSEVGERWLEDAWADTDSGPGELAALHDEFGG
                     QANLAVLTRDGVPHHYAGNSENPVFTFRLGRIGVASTALYSIDRSVFAYAARGATNRR
                     LVRLHTAVTLDETGRPTDSHGGRT"
     gene            2475..>2754
                     /locus_tag="AC162A_06860"
     CDS             2475..>2754
                     /locus_tag="AC162A_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010598.1"
                     /translation="MTAVATGEPGIDIGARPTALLPMPQLVRISLYWLGLTAIDAAVG
                     LFITNRLEFDHFVPTESVGTTLFLVGVGGAIVGIIIQPTVGYLSDFTIS"
BASE COUNT          400 a          994 c          970 g          390 t
ORIGIN      
        1 accgccgccg accttgcccg cctcggctcg gacgggacga cgcccgacga cctcgttcgc
       61 cgctcgttcg agttcctgct cgcccgagag ccgaagtcgt cgatcctcgc gaggttcgac
      121 ctccccctga tcggccgcta cttccccgag tacgaggcgt cgatcacgcg cccgcgatag
      181 cgggggccgc cgcgcggaac gcctcgaggc cacgctcgac catcgcgtcg ctcgccgtga
      241 gcgagacccg gaaccagccg ggcgactcga cgatcgtgcc cggcagcacg agcgcgccga
      301 gctccgcgag gcgatcggtg aacgcgaggt cgtcgggatc gggcgagtgg accatcaggt
      361 agaaggtgcc ctcggggcgg gtgacctcgt agcccatctc gcgcagcgcg gggaccatcc
      421 ggtcgcgccg ggcctggagc acggcgatgt cgaccgacag cccttcgagg tcgcggatcg
      481 agtgctggag cagcgcattc ggaaagcccc agcccgcggc cagctgctgg acgaagatcc
      541 ggtagccgac cgtttgcttg tccgggaacg acggatggag ggccacgaac ccgatccgct
      601 gccccggcgc cagcgtcgtc ttgccgtacg tgtagatgag cgcggtcgcg tcgtagtcga
      661 cggccgggct ccggaacgcg atcccgtcga acacgatccg gttgtaggac tcgtcggaga
      721 tcagcgtgat tgtccgcccg ttgcgctccg aggcggcgcg aaggacgtcc cccaaggccc
      781 gaagctccgg ctcgcgatag atgcggccgg tcgggttgtg cgggctgttg acgatgatgg
      841 cgcgcgtgcg cggggtgatc gcggcggcca cggcatcggg atcgaggtcg aaggtcggcg
      901 tggcgaggtc gacgcggacg gcgcttgcac ccgacgacgc gatcatcagc tcgtagaaga
      961 accacggcgg ggagaggtag atcacctcgt cgccgacgtc gcacagggcc cggatcgtca
     1021 ccgcaagggc gccgaacgcg ccggcggtca tcgccacgtc ctcgggcgcg taggggagcc
     1081 cggtccgccg ccggagcgac tccacgacca cctggcgcgg ctcctcctcg ctcatcttgt
     1141 acgcgaacca gtccttgtcc cgcggcacgg cgttgcgctg cagagcctcg acaaggccgg
     1201 gcagcggcag ctcctggggg ttgccgaacg tgaagtcggc gatgcccgga tcgaggtggt
     1261 ggcgctccca ggagccgttg cggaaccacg agaacacgtg ctcgacgctc gcgaccatgc
     1321 ggtccgtgcg ccccagggtg tcggccaatc cgggatcctc caagagtccc tgcacgatcg
     1381 cccacgtccg tcgcggcgtc aaccacgggc cggcgggacg ggtcgcttcg cgcaggacgg
     1441 cgctggcggt atggttcacg tcaagcagaa ccatcgtcgt gtgacggcgc cgagcgtcca
     1501 cgggacgccg attggcgatg tgagcgtcga ccgccgcacg agacggcagc gccgtggcgc
     1561 cgtccgatgc cccggtctcg gccggcgtcg accgagcgct gcccccgagg cgctccaacg
     1621 gcctggctcg gcgggcgccg ccgttcgagc tggcgccacc gcgcgccgat catgtcgcgt
     1681 ggttggaacc attcgatgtg gcaccagatg ggcagtcggc gaggcagctg cgagcctatg
     1741 atccgcgcga tgtgcgagca gttcgtggcg cgggcggccg agcccttccg gctcgacgag
     1801 ctgtggccgt tcgccgagcg cctcgagcgt tacggactcg ccgggttcgg ctggggcgcc
     1861 acctggctgc gcgccggcgg cggcctcgag acccaccgcc acacgacggc gttctgcgac
     1921 gatcccgcgc gggcccggat cggggccatc gagaccacga gcctgctcgt ccacctccgc
     1981 cgcccgtccc gcctcagcac cctcgacctg cccgacaccc agccgttcga cgacccggcc
     2041 gggcgattcg ccttctcgca caacggcgac ctgcgcgact atcgggcggc gcgcgagcgc
     2101 taccggctgc aggggcggat cttcggccgg gcggacagcg aggtcggcga gcggtggctc
     2161 gaggacgcct gggcggacac cgactcgggc ccgggcgagc tcgccgcgct tcacgacgag
     2221 ttcggcggcc aggccaacct cgccgtgctg acccgggacg gcgtgccgca ccactacgcc
     2281 ggcaacagcg agaacccggt gttcaccttc cggctcggaa ggatcggcgt cgcgtcgacc
     2341 gcgctgtact cgatcgaccg ttcggtgttc gcgtacgcgg cgcgcggtgc cacgaatcgg
     2401 cggctcgtgc gtctccacac cgccgtcacc ctcgacgaga ccgggcggcc caccgacagc
     2461 catggaggcc gcacatgacc gccgtcgcga ccggcgagcc gggcatcgac atcggtgccc
     2521 gccccacggc gctcctgccg atgccccagc tggtccggat ctcgctctac tggctcggcc
     2581 tcacggcgat cgacgcggcg gtcggcctgt tcatcaccaa ccgcctcgag ttcgaccact
     2641 tcgtgcccac cgagagcgtc gggaccacgc tgttcctggt cggggtcggc ggggcgatcg
     2701 tggggatcat catccagccc accgtcggct atctcagcga cttcaccatc agca
//
LOCUS       DBNHXO010000329         2663 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1679280, whole genome shotgun sequence.
ACCESSION   DBNHXO010000329 DBNHXO010000000
VERSION     DBNHXO010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1679280"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="AC162A_06865"
     CDS             complement(<1..304)
                     /locus_tag="AC162A_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010599.1"
                     /translation="MSTRDGFDGFLSDWLDDKAGRGAPDYVDEILVLTTRTRQRPAWS
                     SLERWLPVQTTLRFAPVPRLGWVLLISLAIAAAVGIAILAVGSRSPHVPPPFGPARN"
     gene            complement(301..858)
                     /locus_tag="AC162A_06870"
     CDS             complement(301..858)
                     /locus_tag="AC162A_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5010600.1"
                     /translation="MQLELVERARRGDHDAFAVLARAAVFRLDAAARLILRDPDQAKD
                     AVQETLVRAWRDLPTLRDADRFEAWLHRLLVRACIDEARRLRRHRFDIELVAIDMPVL
                     EDPLSLMADRDQLERGFSRLEPEARALIVLHYYLDLPLPEVAGALGIPLGTAKSRLHR
                     SLQFLRAALDADTRPRPELPQGGLA"
     gene            1022..2206
                     /gene="sucC"
                     /locus_tag="AC162A_06875"
     CDS             1022..2206
                     /gene="sucC"
                     /locus_tag="AC162A_06875"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIR5010601.1"
                     /translation="MKIHEADAKSLLVAEGLPVPAFEVARTPTQARAAAVRFLADPGN
                     ATGKVVIKAQVLVGGRGKAGGVKLAGTADEAEDVAAQILALEIKGLPVRRVLVGPAAE
                     IVKEYYLSVVLDRATRRLMFIGSAEGGVEIEQVAVDNPEAIVYEHADPLLGLPDYAAR
                     ELAFKIGFGPHWKAGAAIAKGLLRTMLAYDADLVEINPLAVIREHNADGSEVERLVCL
                     DAKITLDDSALARHPQLEALRDLDAEDPTDVEARRYDLTFIKLAGDIGCMVNGAGLAM
                     TTMDLVKRAGGEPANFLDIGGGAKSDRVAAAMRLILDDPNVKAILVNIFGGITRGDEV
                     ARGLIEARAQQERAVPMVVRIVGTNAVEAAELLEAASFVTAGSLDEAAAKAVAAAKGA
                     AA"
     gene            2203..>2663
                     /locus_tag="AC162A_06880"
     CDS             2203..>2663
                     /locus_tag="AC162A_06880"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009361 - succinate-CoA ligase complex
                     (ADP-forming) [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004775 - succinate-CoA ligase
                     (ADP-forming) activity [Evidence IEA];
                     GO_function: GO:0004776 - succinate-CoA ligase
                     (GDP-forming) activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR5010602.1"
                     /translation="MSILIGRDTRLVVQGITGREGEFHSRAMLEYGTPLVAGVTPGKG
                     GQTAIDGKVPVFNTVAEAVAATGANTSCIFVPAGGAPDAVLESVAAEIGTIFCITEGI
                     PALDMIPVVEEVRLAGARLIGPNCPGATSPGRAKVGIIPGSIHREGRVGVVS"
BASE COUNT          401 a          935 c          979 g          348 t
ORIGIN      
        1 cgttgcgggc cggcccgaag ggcggcggaa cgtggggcga ccgtgagccg accgcgagga
       61 tcgcgatccc gaccgcggcc gcgatcgcca gcgagatgag gagcacccag cccagcctgg
      121 gcacgggtgc gaagcgcagc gttgtctgca cggggagcca cctttcgagg ctcgaccatg
      181 ccgggcgctg gcgggtccgt gtcgtgagga cgaggatctc gtcgacgtag tcaggcgccc
      241 cgcgcccggc cttgtcgtcg agccagtccg acaggaaccc gtcgaagccg tcgcgcgtgc
      301 tcatgcgaga ccaccctgtg gcagctccgg ccgtggccgg gtgtcggcgt cgagcgccgc
      361 ccgcaggaac tggagcgacc gatggagacg cgatttcgcc gtccccagcg ggatccccag
      421 cgcgccggca acctccggca agggcaggtc gaggtagtag tgaaggacga tgagggcgcg
      481 tgcctccggc tcgaggcggc tgaaaccgcg ctccagctgg tcacggtcgg ccatgagcga
      541 cagcgggtcc tcgaggaccg gcatgtcgat ggcgacgagc tcgatatcga agcgatggcg
      601 ccggagccgc cgcgcctcat cgatgcaggc gcggacgagc agacggtgga gccacgcctc
      661 gaagcgatcg gcatcgcgca acgtgggcag gtcgcgccag gccctgacga gcgtctcctg
      721 gacggcgtcc ttggcctggt cggggtcacg gaggatgagc cttgcggcgg cgtcgagccg
      781 gaagaccgcg gcgcgggcca gcacggcgaa ggcgtcatga tcgccccgtc gcgcccgctc
      841 cacgagttcc agctgcatca cggccctcgg cggcggcgcg gccgatatgg cagcgctcat
      901 cgagtatggc gaggcgaccc cgtgccatcg ttccgttatc gcccgccaac gtcccgcccg
      961 gcggcccgaa ggctatgatc ggaagccgcc cgagtccccg tcccacgagg tccgccgtcg
     1021 cgtgaagatc cacgaagccg acgccaagtc gctgctcgtc gccgaggggc tccccgtccc
     1081 ggccttcgag gtcgcccgca cgccgaccca ggcgcgcgcc gccgccgtcc gattcctcgc
     1141 cgaccccggc aacgcgaccg gcaaggtcgt gatcaaagcg caagtgctcg tcggcggccg
     1201 gggcaaggcg ggcggcgtca agctcgctgg cacggcggac gaggccgagg acgtcgcggc
     1261 ccagatcctc gcgctcgaga tcaaggggct gcccgtccgg cgggtgctcg tcggtcccgc
     1321 ggccgagatc gtcaaggagt actacctctc agtcgtcctc gaccgggcca cccggcgact
     1381 gatgttcatc ggctccgcgg agggcggcgt cgagatcgag caggtggcgg tcgacaaccc
     1441 cgaggcgatc gtctacgagc acgccgaccc gttgcttggc ctgcccgact acgccgcgcg
     1501 ggagctggcg ttcaagatcg ggttcgggcc ccactggaag gccggcgcgg cgatcgcgaa
     1561 gggcctcctg cggacgatgc tcgcgtacga cgcggacctc gtcgagatca acccgctggc
     1621 ggtgatccgc gagcacaacg ccgacgggtc cgaggtcgag cgcctcgtct gcctcgacgc
     1681 caagatcacg ctcgacgact ccgccctcgc ccgccatccg cagctggagg cgctgcgcga
     1741 cctcgacgcc gaggacccga cggacgtcga ggcgcggcgc tacgacctga cgttcatcaa
     1801 gctcgccggc gacatcggct gcatggtcaa cggcgccggg cttgcgatga cgacgatgga
     1861 cctcgtgaag cgggccggcg gcgagccggc gaacttcctc gacatcggcg gcggcgcgaa
     1921 gtccgaccgt gtcgcggcgg cgatgcggct gatcctcgac gatcccaacg tcaaggcgat
     1981 cctcgtgaac atcttcggcg gcatcacccg cggcgacgag gtcgcccggg gcctgatcga
     2041 ggcccgcgcc cagcaggagc gcgcggtgcc gatggtggtc cgcatcgtgg gcaccaacgc
     2101 ggtggaggcc gccgagctgc tggaggccgc gagcttcgtg accgccggca gcctcgacga
     2161 ggcagccgcg aaggccgtcg cggcggcgaa gggggcggcg gcgtgagcat cctgatcggc
     2221 cgcgacaccc ggctcgtcgt ccagggcatc accgggcgtg agggtgagtt ccacagccgc
     2281 gccatgctcg agtacggcac gccgctggtc gcgggcgtca cgccgggcaa gggtgggcag
     2341 accgcgatcg acggcaaggt acccgtgttc aacacggtcg ccgaggcggt cgccgcgacg
     2401 ggcgccaaca cctcgtgcat cttcgtaccc gccgggggcg ccccggacgc ggtgctcgag
     2461 tcggtcgccg cggagatcgg caccatcttc tgcatcaccg aggggatccc cgcgctggac
     2521 atgattccag tggtcgagga ggtccgcctg gctggcgccc gcctgatcgg gcccaactgc
     2581 ccgggggcca cgtctcctgg gcgggcgaag gtgggcatca tcccgggctc gatccaccgc
     2641 gaggggcggg tcggcgtggt ctc
//
LOCUS       DBNHXO010000330         3705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4061814, whole genome shotgun sequence.
ACCESSION   DBNHXO010000330 DBNHXO010000000
VERSION     DBNHXO010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4061814"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..449)
                     /locus_tag="AC162A_06885"
     CDS             complement(120..449)
                     /locus_tag="AC162A_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010603.1"
                     /translation="MASSRRTFLVECYGPDIDAGSVAAAGARARAACDRLERVGVDAH
                     YLGAVLVAEDDVVFHAFAGADAAGIEVVCREAGLVHERVVESIAVTTDGSDPDPAAIL
                     AGIRSAS"
     gene            complement(581..3370)
                     /locus_tag="AC162A_06890"
     CDS             complement(581..3370)
                     /locus_tag="AC162A_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5010604.1"
                     /translation="MSEVVGRDAELRAAEGFLDAAGAGFAALVFEGEPGIGKSALLAE
                     VARRAADRGFLVLACRASSSETALTLAALADLLEPVDEAVIDTLPEPQRHAIDAALLR
                     VMPTGDPVEQRLLGSAVRTLLGRLADVRPVLVAIDDAQWLDPSSAATLQFALRRMTGA
                     AVGVAMTRRRGEPSPLRDDDLGEPPAVIRVEVGPLSLGTLHHVLRDSLGSAPARSTLV
                     RIHRTSRGNPLFAIEIMRRLADVGVPPADQPLPVPADIRELVREQVDRLPRSAREALM
                     VAAAAAVPTVALLDGALGRSTRADLAIAAERGIARVDEGTLVFRHPLFATAVYAEADD
                     DLRREAHRRLWRVVDDREEQARHHALAAVGAEPEAADQLELASAAANRRGAPLAAAEL
                     MELALQSTPTDARAAWHRRSMALAGYLQRSGDFVRGRTVLEALVRDAPVGPTRARARL
                     DLAGILFDTNGDASAARHAEGAIADAQGDLEILARAHATLAAVLYEDREAAAAHGRTA
                     KGLLDQVADPDPRVAAQVLGNYVASETARGLPVEPGVLQRALELERLAPMPNVSDRLS
                     AALGAVLVFRGEFDEARGWLEQTYATAVDEGDDGSMAYALSHLPTLELASGNAALAEE
                     LARRHLAVATELGLESQRLQALFNLALVHTHLGHETEARAEVAELLDAAGRAGDRWSR
                     ANGLAVLGMLELALGRPVAAVPPLLEATAIRDDISDDLPRRHEADLVESLVGVGSLDE
                     ARRVADAMATRAEASGSPWRIAQALRSRAVLEAAGGDLDAAVTTLEAALRRDEPSPVA
                     FDRARAFLVLGVVLRRRRERRAGKAALESAVQTFERIGARLWAARAAGELDRIGIRRS
                     PAGALTPSERRVVELAAMGRTNRQVATELYMSPKTVEANLARAYLKLGVRSRAELGAV
                     VASSTRPDALAADPD"
BASE COUNT          528 a         1359 c         1331 g          487 t
ORIGIN      
        1 agccggtagg gtcgcctccg cggctcgcgc ggcgccggat gccgcaacct ccccaccaag
       61 ctgcgtcgtg ttcggctcat ccaccggtcg tgccctccag ctgcgtcacg tggggtcctt
      121 cagctcgcgg accggatgcc cgcgaggatg gccgcggggt ccgggtcgct gccgtcggtc
      181 gtgacggcga ttgactccac aacccgctcg tggacgaggc cagcttcccg gcacacgacc
      241 tcgatgccag cggcgtccgc acccgcgaac gcgtggaaca caacatcgtc ttcggcgacc
      301 agcaccgcgc ccaggtaatg agcgtcgacg ccgacccgct ccagtcgatc gcacgcggcg
      361 cgtgctcgag cgccggcggc cgccacggac ccggcgtcga tgtccgggcc gtagcactcg
      421 acgaggaacg tccgacggga cgaggccatc cagcggctcc atgggtcggt cgctgcgcca
      481 tggctggcgc gttcgaccaa ccccgcgacg ctaggtcacc cgtgtcccca tcggcatcgg
      541 gtggttcccc atgtttgcag gccgccgacg cgccgaacgt tcagtcgggg tcagctgcca
      601 gggcatcggg tcgggtggac gacgcgacca cggcgcccag ctccgcgcgc gaccggacgc
      661 cgagcttcaa ataggcacgg gccaggttcg cctccacggt cttggggctc atgtacagct
      721 cggtggcaac ctggcggttc gtcctgccca tggccgcgag ctcgacgacg cgacgctccg
      781 aaggcgtcag tgcgcccgcc ggcgatcgcc ggatcccgat cctgtccagt tcgcccgccg
      841 ctcgcgccgc ccacaacctg gcgccgatgc gctcaaacgt ctggacggcg gactcgagtg
      901 cggccttgcc tgcccgtcgc tcgcgtcgtc gccggagcac gaccccgagg accaagaacg
      961 cacgggcccg gtcgaatgcc acgggcgatg gctcgtcgcg tcgcagcgcc gcctcgagcg
     1021 tggtgacggc cgcatcgagg tccccgcctg ccgcctcgag gacggcccgg gaccgcagcg
     1081 cctgcgcgat ccgccaaggg ctgccggaag cctccgcccg ggtcgccatc gcgtcggcga
     1141 cccgccgggc ctcgtcgagt gacccgaccc ccacgaggct ctccacgagg tccgcctcgt
     1201 gccggcgcgg gagatcgtcg ctgatgtcgt cgcggatggc cgtcgcctcg agcagtggcg
     1261 gcactgctgc cactggcctg cccagtgcga gctcgagcat tccgaggacg gcgagcccgt
     1321 tggcgcgcga ccatcgatcg cccgcccggc ccgcggcgtc gagcaactcg gcaacctcgg
     1381 cgcgcgcctc ggtctcgtga ccgaggtgcg tatggaccaa cgcaaggttg aacagcgcct
     1441 gcagccgctg cgactcgagc ccaagctcgg tggctacggc caggtgccgc cgggccagct
     1501 cctccgcaag agcggcgttg ccactcgcca gctccagggt cggcaggtgg ctgagggcgt
     1561 aggccatcga accgtcgtcg ccctcgtcga ccgcggtcgc atacgtctgt tcgagccagc
     1621 cgcgagcctc gtcgaactcg cctcggaaga cgaggaccgc gccaagcgcc gcgctcagac
     1681 ggtcgctgac gttgggcatc ggcgcgagcc gctcaagctc gagcgcccgc tggagcaccc
     1741 cgggctcgac ggggagccca cgtgccgtct cgctcgcgac gtagttgccg aggacctgtg
     1801 cggcaacgcg cggatccgga tcggcgacct ggtcgaggag acccttggcc gtcctgccat
     1861 gggcggccgc ggcctctcga tcctcgtaca gcacggcggc caacgtggca tgggcacggg
     1921 cgaggatctc gaggtcgccc tgggcatcgg cgatggcgcc ctccgcatgg cgggccgccg
     1981 aggcgtcgcc gttggtatcg aacaggattc ccgccaggtc gaggcgcgcg cgggcccggg
     2041 tcggtcccac aggggcgtcg cggacaaggg cctccagcac cgtgcgaccg cgtacgaagt
     2101 cgccggagcg ctgcagatag ccggccagcg ccatggatcg ccgatgccat gcggcgcgag
     2161 cgtccgtggg tgtggactgc agcgcgagct ccatcagctc ggcggccgcg aggggagcac
     2221 cgcgtcgatt cgccgccgcc gacgcgagct cgagctggtc ggcggcctcc ggctcggctc
     2281 cgacggccgc gagcgcgtgg tggcgggcct gttcctcccg atcatccacg acccgccaca
     2341 aacgacgatg ggcttcgcgg cgcaggtcgt cgtccgcctc cgcgtacacg gcggtggcaa
     2401 agaggggatg gcggaagacg agggtgccct cgtcgacacg cgcgatccca cgctcagccg
     2461 cgatggcgag gtctgcgcgc gtcgagcgcc cgagcgcccc gtcaagaagc gcgacggtgg
     2521 gcaccgccgc ggccgcggcg accatgagcg cctcgcgcgc ggagcgtggc agacggtcga
     2581 cctgctcgcg cacgagctcg cgaatgtcgg cgggcaccgg cagcggctgg tcggcgggtg
     2641 ggacgccgac gtcggcgagt cgtcgcatga tctcgatcgc gaacagcgga ttgccccgtg
     2701 acgttcggtg gatccggacg agtgtcgaac gcgccggcgc ggatcccagc gagtcccgga
     2761 gcacgtggtg aagcgtcccc aggctgagcg gccccacctc gacgcgaatg acggccgggg
     2821 gttccccgag gtcgtcgtca cggagcggtg acggctcgcc ccgacgccgg gtcatggcca
     2881 ccccgaccgc cgcacccgtc atccgacgaa gggcgaactg caacgtcgcc gcagacgacg
     2941 ggtctagcca ctgcgcgtcg tcgatcgcga ccaggaccgg gcgaacgtcg gcgaggcgac
     3001 cgagcaacgt ccgcacggca ctgccgagca gccgctgctc gaccgggtcg cccgtcggca
     3061 tgacgcgcag cagggcagcg tcgatggcgt gtcgttgcgg ctccgggagc gtgtcgatca
     3121 cggcctcatc gacgggttcc agcaggtcgg cgagcgcggc cagcgtcagg gcggtctcgg
     3181 aggacgacgc tcgacaggcg aggacgagga acccgcgatc ggctgcgcgc cgagcgacct
     3241 cagcgaggag cgcgctcttc ccgatccccg gctcgccctc gaagaccagg gcagcaaacc
     3301 ctgctccggc ggcgtccagg aacccctcgg cggcacgaag ctcggcatcg cggccgacga
     3361 cctcgctcac gttgcgagtg tgctggctcg cccacgcgtg cgcaaccgcc gtggccgtca
     3421 gggcggcgcg cgccgtacgg ttcgctagcg ccgcgctgca cgcccagcgt tcactgcggc
     3481 ggttggaacg cgcgattgat cgcccggcga gcaggtcggc ggataaggcg gcgattcccg
     3541 gtggttcgct cgcctggggt gcaggatcgg gccgccgacc gctggactgc acgccaggcg
     3601 agcagctgac gctgcgcgtg cgcaaccgcg cgcgaacgtt gacggcgcaa cgcggcgcgc
     3661 gatatgctcc atgcgtcccc accggccgcg cgcgacggcg atccg
//
LOCUS       DBNHXO010000331         3618 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1484565, whole genome shotgun sequence.
ACCESSION   DBNHXO010000331 DBNHXO010000000
VERSION     DBNHXO010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1484565"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /gene="whiA"
                     /locus_tag="AC162A_06895"
     CDS             <1..473
                     /gene="whiA"
                     /locus_tag="AC162A_06895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HIR5010605.1"
                     /translation="GLPASWRVRRGQGVVIWKNAESISAFLGRIGASASLLELEARQV
                     ARSLRGELNRVLNAESANLGRSVAASGRQLAAIDALEADGRLGEQPRIVRAVADARRE
                     TPEASLSEIATRLDLHRSAVQRALDRVERLALHDDEGIGRRRPGRPRPAGAPLA"
     gene            482..1264
                     /gene="tpiA"
                     /locus_tag="AC162A_06900"
     CDS             482..1264
                     /gene="tpiA"
                     /locus_tag="AC162A_06900"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR5010606.1"
                     /translation="MRPIVIAANWKMNTTPSDAGELARTIASRTREPGVTRVICPPFV
                     CLPAVRDALAGDDVGVGAQNVHHELAGAFTGEVSVPMLAGLATWVIVGHSERRAQFGE
                     TDDLIGRKLDRAVAAGLRPILCVGEVLAEREAGREVAVVDAQLRGSLEGRDAAALIGA
                     GLVVAYEPVWAIGTGRNATGADAAAMADAIRTSLDALGWQAAAEVPVLYGGSVTSANI
                     GVFLAEPSIDGALVGGASLKPDEMAGIVARAGITAAARGLAG"
     gene            1261..2850
                     /gene="gpmI"
                     /locus_tag="AC162A_06905"
     CDS             1261..2850
                     /gene="gpmI"
                     /locus_tag="AC162A_06905"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR5010607.1"
                     /translation="MTDTAQPSPRARPRPIVLVVLDGFGIGRDPAADAIAAAAMPRWR
                     ALLARWPHAVLGASEGAVGLPEGQMGNSEVGHLNLGAGRPVLQDLPRIDAAIADGSFD
                     ERPALLAACERARETGRLHVVGLVGPGGVHANDRHLVALVRLAAAQGVRSVRIHALLD
                     GRDTPPSSGLGFVRDLETVLAASHPDARIASVGGRYWAMDRDERWDRVERGYDAIVHG
                     VGEHAATAEAAIEAAYGRGETDEFVAPTVIDGADGALRDRDPIVHVNFRADRARQLTH
                     ALADTGFDAFDRTGPAGEPPPHDLLVVTMTAYEAGLPVEVAFGPEEARSLAQAFSEAG
                     WTQFHVAETEKYAHVTYFFNGGREAAWPGEERRLVPSPRVATYDLQPEMSAAGVTDEL
                     VAAIESGRYDFIVANYANPDMVGHTGRWEATIIALEALDACLGRVADAVARIEAGDPA
                     GPGAALLITADHGNADELRDVAGRPVTAHSLNPVPLLAAGRRLDGRAIHDGVLADVAP
                     TILDLAGLPRWDGMTGRSLLD"
     gene            2885..3157
                     /gene="secG"
                     /locus_tag="AC162A_06910"
     CDS             2885..3157
                     /gene="secG"
                     /locus_tag="AC162A_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR5010608.1"
                     /translation="MPDRHHPEVNAPVSPILAVGQILLSIALIAAILMQARGTGLSGT
                     FGGDSAVYRSRRGIERRLWQFTILLLVLFVVFCLVAFVFGPAPAAS"
     gene            3200..>3618
                     /locus_tag="AC162A_06915"
     CDS             3200..>3618
                     /locus_tag="AC162A_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5010609.1"
                     /translation="MSITRRDSAVVAVLVLSLVILGGVIAVPQAAPAATPQPTEPPTL
                     PPPATYREGVVGVPEAIMPVAARTRAERTLVSLIFSGLVRLGPGNTYEPDLAESWTTD
                     AKGKTWTFTIRDDATWQDGVPVTADDVVYTIEALKNP"
BASE COUNT          448 a         1361 c         1326 g          483 t
ORIGIN      
        1 tgggcctgcc ggcgtcgtgg cgggtgcgcc gcggtcaggg cgtcgtgatc tggaagaacg
       61 ccgagtccat ctccgccttt ctgggccgta tcggggccag cgcgtcgctc ctggagctcg
      121 aggcgcgcca ggtcgcccgc agcctgcgcg gcgagctcaa ccgcgtcctc aacgccgagt
      181 cagccaacct cgggcggtcg gtcgccgcat cgggccgcca gctcgcggcg atcgacgcgc
      241 tcgaggcgga cggccggctg ggcgagcagc cccggatcgt gcgcgccgtc gcggatgccc
      301 ggcgtgagac gcccgaggcc tcgctgtccg agatcgccac gcgcctcgac ctccaccggt
      361 ccgccgtcca gcgcgcgctg gatcgcgtcg agcggctcgc gctccacgac gacgagggga
      421 tcgggcggcg gcgtcccggc agaccccggc cggcgggcgc gcctctggca tgatcagggc
      481 gatgcgcccg atcgtgatcg ccgccaactg gaagatgaac accaccccgt ccgatgcggg
      541 cgagctggcg cggacgatcg cgtcgcgcac ccgcgagccg ggcgtcaccc gcgtcatctg
      601 cccgcccttc gtgtgcctgc cagccgtgcg ggatgcgctg gcgggcgacg acgtcggcgt
      661 cggggcccag aacgtccacc acgagctcgc cggcgcgttc acgggcgagg tgtcggtgcc
      721 gatgctggcc gggctcgcaa cctgggtcat cgtcggccac tcggagcgcc gggcgcagtt
      781 cggcgagacc gacgacctca tcggccgcaa gctcgaccgg gccgtcgcgg cggggctgcg
      841 cccgatcctg tgcgtcggtg aggtgctcgc ggagcgcgag gcgggccgcg aggtcgcggt
      901 ggtcgacgcc cagctgcgcg ggtcgctcga gggccgggat gccgcggccc tgatcggcgc
      961 cggcctcgtc gtcgcgtacg agccggtctg ggcgatcggg accggccgga acgcgaccgg
     1021 agccgacgcc gcggcgatgg ccgacgcgat ccgcacgtcg ctcgacgccc tgggctggca
     1081 ggcggccgcc gaggtcccgg tgctctacgg cggcagcgtc acctcggcga acatcggggt
     1141 gttcctcgcc gagccctcga tcgatggcgc gctcgtcggt ggggcgtcgc tcaagcccga
     1201 cgagatggcc ggcatcgtcg cccgggccgg catcaccgcg gcggcccggg gcctcgccgg
     1261 gtgacggaca cggcgcaacc gtcgccgcgc gcccgtcccc gcccgatcgt cctcgtcgtc
     1321 ctcgacggct tcggcatcgg ccgcgacccg gccgccgacg ccatcgcggc ggcggccatg
     1381 ccccgctggc gagcgctcct ggcccgctgg ccccacgcgg tcctcggggc gtccgagggc
     1441 gcggtggggc ttcccgaggg gcagatgggg aactcggagg tcggccacct caatctcggc
     1501 gccgggcgcc cggtgctcca ggacctgccg cgcatcgacg cggcgatcgc cgacggctcg
     1561 ttcgacgagc ggccggccct gctggccgcc tgcgaacggg cgcgggagac gggtcgcctg
     1621 cacgtcgtcg gcctcgtcgg gcccggcggg gtccacgcca acgaccggca cctcgtcgcg
     1681 ctggtacgcc tggccgcggc gcagggcgtg cggtcggtgc gcatccacgc gctgctcgac
     1741 ggccgcgaca ccccaccgag ctcgggactg ggcttcgtcc gcgacctcga gacggtgctg
     1801 gcggcgtcgc atcccgacgc ccggatcgcg tcggtgggtg gccggtactg ggcgatggac
     1861 cgcgacgaac gctgggaccg ggtcgagcgc gggtacgacg cgatcgtcca tggggtgggg
     1921 gagcatgccg ccaccgcgga ggcggccatc gaggcggcct acgggcgggg cgagacggac
     1981 gagttcgtcg ccccgaccgt gatcgacggg gcggacggag cgctgcgcga tcgggatccg
     2041 atcgtgcacg tcaacttccg ggcggaccgc gcgcgccagc tgacccacgc cctcgccgat
     2101 accgggttcg acgccttcga ccggactgga ccggccggcg agccgccgcc gcacgacttg
     2161 ctcgtggtca cgatgaccgc gtacgaggcc ggcctcccgg tggaggtcgc cttcgggccc
     2221 gaggaggcac gatcgctggc ccaggcgttc tccgaggccg gttggaccca gttccacgtc
     2281 gccgagaccg agaagtacgc ccacgtgacc tacttcttca acggcggccg cgaggccgcc
     2341 tggccgggcg aggagcggcg gctggtgccg tcgccccggg tggccaccta cgacctccag
     2401 cccgagatga gcgccgccgg cgtgaccgat gagctggtcg ccgcgatcga gtccggccgc
     2461 tacgacttca tcgtcgcgaa ctacgcgaac cccgacatgg tcggccacac gggccgctgg
     2521 gaggccacga tcatcgcgct cgaggcgctc gacgcgtgcc tgggtcgcgt cgccgatgcc
     2581 gtggcgcgga tcgaggccgg cgatccggcc gggcccgggg ccgcactgct gatcacggcc
     2641 gaccacggca acgccgacga gctgcgcgat gtcgccggtc gcccggtgac cgcccattcg
     2701 ctcaacccgg tgccgctgct cgccgccggc cggcggctcg acgggcgcgc catccacgac
     2761 ggcgtcctcg cagatgtcgc cccgacgatc ctcgacctgg cggggctccc gcgctgggac
     2821 gggatgaccg gacggtcgct gctggactag ggccgggcag ggtcggagcc acggttctgc
     2881 taccatgccc gaccgtcacc acccggaggt caacgcaccc gtgagcccga tcctggccgt
     2941 cggccagatc ctgttgagca tcgcccttat cgccgccatc ctgatgcagg cgcgcggcac
     3001 aggcctgtcc ggaaccttcg gcggtgattc ggccgtctac cgcagccgcc gcggcatcga
     3061 acgccgcctg tggcagttca cgatcttgct cctggtgctg ttcgtcgtgt tctgtctggt
     3121 cgcgttcgtc ttcgggccgg ctccggccgc aagctaggcg caggcccggg gctcctctcc
     3181 aacccgcgtc tcgaacctga tgtccatcac ccgccgcgac agcgcggtcg tcgccgtcct
     3241 cgtcctctcg ctcgtgatcc tcggcggcgt gatcgccgtc ccgcaggcgg ccccggcggc
     3301 gaccccgcaa cccacggaac cgccgacgtt gccgcccccg gccacctatc gcgagggcgt
     3361 ggtcggcgtt cccgaggcga tcatgccggt cgccgcgcgc acgcgagccg agcgcaccct
     3421 cgtcagcctc atcttcagcg gcctggtgcg cctcggaccg ggcaatacct acgagcccga
     3481 cctcgccgag tcctggacga ccgacgccaa gggcaagacc tggacgttca cgatccgcga
     3541 cgacgcgacc tggcaggacg gcgtccccgt gacggccgac gacgtggtgt acacgatcga
     3601 ggcgctgaag aaccccga
//
LOCUS       DBNHXO010000332         5164 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_295041, whole genome shotgun sequence.
ACCESSION   DBNHXO010000332 DBNHXO010000000
VERSION     DBNHXO010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_295041"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="AC162A_06920"
     CDS             <1..429
                     /locus_tag="AC162A_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010610.1"
                     /translation="VAIDDGHTVSLDELEVARRDLSLIEVAGRRGDPQRRLRTVAEHV
                     RLEVGPFRVTGNLHRSPSAHPLNALNRWSRFLPVTEAYVEIDGSTSEPATHEVILVNR
                     DRIGTYDVLIDAAPIWVPAPPEAEPPPEAAPQPEAAQPIA"
     gene            488..1225
                     /locus_tag="AC162A_06925"
     CDS             488..1225
                     /locus_tag="AC162A_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5010611.1"
                     /translation="MTPAPAAVRDQAPILVVDDDPKILALVRTYLERERYRVVTAVDG
                     RAALDAIAEHPRLVVLDLMIPEIDGIEVIRRARQFGDVPILVLSARGAVADRIQALTE
                     GADDYLPKPFSPAELVVRVATILRRTERGGADDIVLAMGGLELDLDRHRVTIDGAEVA
                     LSALELRLLAALMEAAGRVLTRDQLLDAIHGAGEGDVLDRSIDQYVRRLREKLGDDPA
                     DPAYVATVRGAGYRAAGPVERRVAGRS"
     gene            1209..2294
                     /locus_tag="AC162A_06930"
     CDS             1209..2294
                     /locus_tag="AC162A_06930"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5010612.1"
                     /translation="MPDGRERLMGRRDGLSARILVAALAVAGIALAIVGIGVLRVGAA
                     SFEALMMAAGESADHAREMFDRSVTIVFGLAALVAAIVAIALAMALSRRLAQPLERIS
                     AAAARIADGDLEARVPEEGPAELRALAASYNAMAARLAQQEAVRREFIINASHELRTP
                     LTNLQGYLEALRDGVLPPDPATFDSLREEVDRLTRLAASLDVLAGVEGSQGVARPVDV
                     GAIVAASTDLVAPALARRSIGLRLDAPAGFIAHARPDELTQVVANLLQNAVRYTPDGG
                     EVQVTVDGADGGVRVRVANTGPGIPEADLPRVWERFYRVEKSRDRERGGAGIGLAIVR
                     QLVEAAGGRVGVTSEGGWTTFWLTLPA"
     gene            2362..2955
                     /locus_tag="AC162A_06935"
     CDS             2362..2955
                     /locus_tag="AC162A_06935"
                     /inference="COORDINATES: protein motif:HMM:NF021996.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HIR5010613.1"
                     /translation="MPRPSRPIVVAVAIAAVVIGAPIAWYLGSPVFLRTELQEPAIEA
                     FEPPAATSTPAPTPTAAAVTDGPAPSTAVPSPAASAIAAVPVWTPDPPRTGRFSGTDD
                     FHFGRGTAILREVEAGRWVVRLEDFAVRNGPDLYVVLSPDPGGYTDDAIEVGRLKATD
                     GNFNMRVPDGTDVSEVRSVLIWCKQFSHLFAVAPLEG"
     gene            complement(3014..3226)
                     /locus_tag="AC162A_06940"
     CDS             complement(3014..3226)
                     /locus_tag="AC162A_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010614.1"
                     /translation="MQDIAPIVQLVLVLAAVASPVILFARLVSGGEQGSLASLFTAPD
                     TYTWPRGVQEEDPKPWVLGATVPAAA"
     gene            complement(3374..>5164)
                     /locus_tag="AC162A_06945"
     CDS             complement(3374..>5164)
                     /locus_tag="AC162A_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014026.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR5010615.1"
                     /translation="DIATGVVATGKHLVGHGLAEGGLNQAPAHIGPRELRDEQLLPFE
                     AAVRDAGLACMMPAYCDVDGLPCHVSEELLTSILRREWGFDGIVASDYTAVQMLVGQH
                     RLTSDLGTAASMALRSGMDSELPTSAGYAGPLKAAIADGRIDLAFVDLAVERNLRLKF
                     RLGLFERPYVDVPSVAFLDQIHAEEQSLARDLVRRSIVLLENDGVLPLADASSGIAVI
                     GPIADSGRDLMGDYAHLPHIETLAELRHQANPFGFPSSEVIQPTDESGAWPTILGALR
                     ERFGAANVTYARGTGLRDGEDEEIAEAAAAARAAGIAIVVLGERSGLTNDATTGESRD
                     RRDLRLLGRQQELLEAVVATGTPTILLVVSGRPLALEWAAAHCAAVVLAWVPGEAGPA
                     AIAEVIAGDVDPGGRLPITMPRHVGQLPLTYRHHPTGGRSNWKIDYVDGLVSPMWSFG
                     QGRSYTTFAMDRLRVDRSTLDTAGDTVTIDVDVTNTGSRPGDEVVQLYVRDEEASVAR
                     PVLELRGFRRVSLEPGECRTVTFRLSTEQLAYVGADYRRVVEPGLVRIFVGRSAVELP
                     LSADLHLAGPTVELVERTRYLTEASDAREG"
BASE COUNT          672 a         1884 c         1911 g          697 t
ORIGIN      
        1 gtcgccatcg acgacggcca cacggtcagc ctcgacgagc tcgaggtcgc gcggcgggac
       61 ctgagcctga tcgaggtcgc gggccggcgg ggcgacccgc agcgtcgcct gcgcacggtc
      121 gccgagcacg tgcgcctcga ggtcggccca ttccgggtga cgggcaatct gcatcggtcc
      181 cccagcgccc atcccctcaa cgctctcaac cggtggtcgc ggttcctgcc agtgaccgag
      241 gcgtacgtcg agatcgacgg gtcgacctcg gagccggcga cccacgaggt catcctcgtc
      301 aaccgcgacc ggatcggcac ctacgacgtg ttgatcgacg ccgcgccgat ctgggtcccc
      361 gcgccgcccg aggccgagcc gccgcccgag gccgcgccgc agcccgaggc cgcgcagccg
      421 atcgcctgat cgcgtcacgc gcgcgtcacc gttgcccgcc aggcttggcg cggcgatgat
      481 gccagccatg acgcccgccc ccgccgccgt ccgtgaccag gcgcccatcc tggtggtcga
      541 cgacgacccg aagatcctgg ccctcgtgcg cacctacctc gagcgggagc gctatcgcgt
      601 ggtgaccgcg gtcgacggac gcgccgccct cgacgccatc gccgagcacc cgcgcctcgt
      661 cgtgctcgac ctgatgatcc cggagatcga cggcatcgag gtgatccgtc gggcgcgtca
      721 gttcggcgac gtgccgatcc tcgtgctgtc ggcgcgcggc gcggtggccg accggatcca
      781 ggccctcacc gagggcgccg acgactacct ccccaagccc ttctcgccgg ccgagctggt
      841 cgtgcgcgtg gccacgatcc tgcggcggac cgagcgcggc ggcgctgacg acatcgtcct
      901 cgccatgggc gggctcgagc tcgacctcga ccgccaccgg gtcacgatcg atggtgccga
      961 ggtcgcgctg agcgcgctcg agctcaggtt gctcgcggcc ctcatggagg ctgccgggcg
     1021 cgtcctcacc cgcgaccagc tgctggacgc gatccacggc gccggcgagg gggatgtcct
     1081 cgaccgctcg atcgaccagt acgtgcggcg gctgcgcgag aagctgggcg acgaccccgc
     1141 cgaccctgcc tacgtcgcca ccgtgcgggg agccgggtat cgcgccgccg ggcccgtcga
     1201 gcgccgcgtt gccggacggt cgtgaacgcc tgatggggcg gcgcgacggg ctgtcagcgc
     1261 ggatcctggt cgccgcgctg gccgtggcgg gcatcgcgct cgcgatcgtg gggatcggcg
     1321 tgctgcgggt cggcgcggcc tcgttcgagg ccctgatgat ggcggccggc gaaagcgccg
     1381 accacgcccg cgagatgttc gaccgcagcg tcacgatcgt gttcgggctg gcggcgctgg
     1441 tggcggcgat cgtggccatc gccctcgcga tggccctgtc ccgccggctc gcgcaacccc
     1501 tggagcggat ctccgcggcc gcggcgagga tcgcggacgg cgacctcgag gcgcgtgtcc
     1561 ccgaggaggg accggcggag ctgcgcgcgc tcgccgcgtc ctacaacgcg atggccgcgc
     1621 gcctcgccca gcaggaggct gtccggcgcg agttcatcat caacgcctca catgagctac
     1681 ggacgccgct cacgaacctg cagggctacc tcgaggcgct gcgggacggc gtgctgccgc
     1741 cggatccggc gacgttcgac tcgctgcggg aggaggtgga ccggttgacc cgcctcgcgg
     1801 cgtcgctgga cgtgctcgcc ggcgtcgagg ggtcccaggg cgtcgcccgg cccgtcgacg
     1861 tcggcgcgat cgtcgcagcg tccaccgacc tggtcgcccc ggcgctcgcg cgacgctcga
     1921 tcgggctgcg gctggacgcg ccggccggct tcatcgccca cgcgcggccg gacgagctca
     1981 cccaggtggt ggccaacctg ctccagaacg ccgtccgcta cacgcccgac ggtggcgagg
     2041 tccaggtgac ggtcgacggc gccgacggtg gcgtccgggt gcgggtcgcc aacaccggcc
     2101 cgggcatccc cgaggccgac ctgccgcgcg tctgggagcg cttctatcgg gtggagaagt
     2161 cgcgcgaccg cgagcgcggc ggcgcgggca tcggcctcgc gatcgtgcgc cagctggtcg
     2221 aggcggccgg cggccgggtc ggggtcacca gcgagggcgg ctggacgacg ttctggctga
     2281 cgctcccggc ctgagcgtca tcgtcgcgtc accgggctgg tcgatgctcc ggtcctacgc
     2341 ccgtcgttcc ggagcccgcc gatgccccgt cccagccgcc cgatcgtggt cgcggtcgcc
     2401 attgccgccg tcgtcatcgg cgcgcccatc gcctggtacc tcggttcgcc ggtgttcctg
     2461 cggactgagt tgcaggagcc ggccatcgag gcgttcgagc cccccgccgc gacctcgacg
     2521 ccggcaccga cgcccaccgc ggccgcggtc accgacggac cggccccgtc gacggctgtc
     2581 ccgagccccg cggcatcggc catcgcggcg gtgcccgtct ggacgcccga tccgccgcgg
     2641 acgggccgct tctcggggac ggacgacttc cacttcggtc gcggaacggc gatcctgcgc
     2701 gaggtggagg cgggacggtg ggtcgtccgg ctcgaggact tcgcggtccg caacggcccc
     2761 gacctctacg tcgtgctctc gcccgacccg ggcggctaca ccgacgacgc catcgaggtg
     2821 ggccggctca aggcgaccga cggcaacttc aacatgcggg taccggacgg aaccgacgtg
     2881 tcggaggtcc ggagcgtcct catctggtgc aagcagttct cacacctgtt cgcggtcgcg
     2941 ccgctcgaag gctgagcaga cggacgcccg cgcggccttc gagggtatgg ccggtcgcgg
     3001 gaccggctcc gagctaggcg gccgccggaa cggtggcgcc gaggacccac ggcttcgggt
     3061 cttcctcctg gacgccgcgc ggccaggtgt atgtatccgg ggcggtgaac agggacgcga
     3121 gcgacccttg ctcgccgccg ctcacgaggc gggcgaagag gatgaccggg ctggcgacgg
     3181 ccgcgagcac gaggaccagc tggacgatgg gggcgatgtc ttgcacgttg gtggctccgt
     3241 cgattggccc aggtgtggaa gggccgttga cgacagcctc gcggaccggg cggcgcgcgg
     3301 tgatggacgg cgatggccgc gtcgtctacg tcaaacggcg acgggaccac ccgtcgagcg
     3361 tggacgccgg gcgtcagccc tctcgggcgt cgctcgcctc ggtgaggtac cgcgtccgct
     3421 cgacgagctc cacggtgggg ccggccaggt ggaggtcggc cgacagtggg agctcgaccg
     3481 ccgaccggcc gacgaagatc cggacaaggc cgggctcgac gacgcgccgg tagtcggcac
     3541 cgacgtacgc cagctgctcc gtcgagaggc ggaaggtcac cgtccggcac tcgcccggct
     3601 cgagcgagac gcgccggaag ccgcgcagct cgaggaccgg tcgggcgacg ctcgcctcct
     3661 cgtcccggac gtatagctgg acgacctcat ccccggggcg cgacccggtg ttggtgacgt
     3721 cgacgtcgat cgtgaccgtg tcgcccgccg tgtcgagggt cgagcggtcg acccgcagcc
     3781 ggtccatggc gaacgtggtg tacgagcgac cctggccgaa cgaccacatg ggcgacacga
     3841 ggccgtcgac gtagtcgatc ttccagttcg atcgcccacc cgtcgggtgg tggcggtagg
     3901 tcagcggcag ctggccgacg tggcgcggca tcgtgatcgg cagccggccg cccgggtcca
     3961 cgtcgcccgc aatgacctcc gcgatggccg cgggcccggc ctcgcccggg acccacgcga
     4021 ggacgacggc cgcgcagtgc gccgcggccc actcgagcgc gagtggccgc ccgctcacga
     4081 ccagcaggat cgtcggggtc ccggtcgcga ccaccgcctc gagcagctct tgctgccggc
     4141 cgaggaggcg gaggtcgcgc cggtcacggg actcgccggt ggtggcgtcg ttggtcaggc
     4201 ccgagcgctc gccgagtacg acgatcgcga tccccgcggc gcgcgcggcg gccgccgcct
     4261 cggcgatctc ctcgtcctcg ccgtcgcgca ggcccgtgcc acgcgcgtac gtgacgttcg
     4321 ccgcgccgaa gcgctcgcgg agggcgccca ggatcgtggg ccacgcgccg gactcgtcgg
     4381 tcggctggat cacctcgctc gacgggaagc cgaacgggtt ggcctggtgg cgcagctcgg
     4441 ccagggtctc gatgtggggc aggtgcgcgt agtcgcccat gaggtcgcgg ccgctgtcgg
     4501 caatcggccc gatgacggcg atcccgctcg acgcgtcggc caggggcagc acgccgtcgt
     4561 tctcgagcag cacgatcgac cgccggacga ggtcccgtgc gagcgactgt tcctcggcat
     4621 ggatctggtc gaggaatgcg acggacggca cgtcgacgta gggccgctcg aacaggccga
     4681 gccggaactt gagccgcagg ttgcgctcga cggcgaggtc gacgaacgcg aggtcgatcc
     4741 ggccatcggc gatggccgcc ttgagcggcc ccgcgtatcc cgcggaggtc ggcagctcgc
     4801 tgtccatgcc ggaccgcagc gccatcgagg ccgccgtgcc caggtcgctc gtcaggcggt
     4861 gctggccgac cagcatctgg accgccgtgt agtcggacgc cacgatcccg tcgaaccccc
     4921 actcgcgacg caggatcgag gtcagcagct cctccgacac gtggcagggg aggccgtcga
     4981 cgtcgcagta ggcgggcatc atgcacgcga ggccggcgtc gcgaacggcg gcctcgaacg
     5041 ggagcagctg ctcgtcgcgc agctcgcgcg gcccgatgtg ggccggggcc tggttgagac
     5101 caccctcggc gagcccgtgg cccacgagat gcttgcccgt cgcgacgacc ccggtcgcga
     5161 tgtc
//
LOCUS       DBNHXO010000333         2509 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3664972, whole genome shotgun sequence.
ACCESSION   DBNHXO010000333 DBNHXO010000000
VERSION     DBNHXO010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3664972"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="AC162A_06950"
     CDS             <1..822
                     /locus_tag="AC162A_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010616.1"
                     /translation="LAASGKLADAVDAARRGLELAEGRERRRTLEQLIERLRASEPAE
                     PGRVALERALVVLEGPAVEARRQSPPAHAKSTPAKAAAASPVEPPVKRHKATPAVETA
                     PEVVEATEVAAVEAPAGSVPAVAPPVEEAAGPAETEPAPEPVPAPRRALDRDLPPDLD
                     LAELALAAEETLERRVQGVGLDQLLDLASAYRRDGHAEAALDTCYLALSIAPDDLSLH
                     LALVELYDERGWSTLATEKLDLLDRLAALDADESHQALLAGTRVARGQGGPPARS"
     gene            866..1921
                     /gene="cdaA"
                     /locus_tag="AC162A_06955"
     CDS             866..1921
                     /gene="cdaA"
                     /locus_tag="AC162A_06955"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIR5010617.1"
                     /translation="MLPFVESLVRQVTLTTLLDIAITALFIYWLFSLIRGTRAVTLVI
                     GVSILLGVYALAQFLQLRLFTQILQAGAVVGLFALVVVFQPELRRGLERIGRVGTFGW
                     LIAPSGQRTIQHVAAEVAKAAGQLSREGHGALIVLERETGLEEIAETGVMIHADLSSE
                     LLCTIFAPRTSLHDGAVVIRDDRILAAAALLPLTEMTLSERFGTRHRAALGITEDTDA
                     VAVVVSEESGQMSVVERARIVRVPSETQLERALTALLEAPATPPGLSRRRGSVFRGRT
                     RGRLRIRTTPGRVDAPPATPGTPAGGQPAASGGSGLSSAAMLPTNGGPAPDPGPIPET
                     PVGDENGSGAAAAVAGR"
     gene            1918..>2509
                     /locus_tag="AC162A_06960"
     CDS             1918..>2509
                     /locus_tag="AC162A_06960"
                     /inference="COORDINATES: protein motif:HMM:NF019561.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR5010618.1"
                     /translation="MSLGSRLQRAVRFVVHNWPLKLAAIALATLLYAGLVASQDSITF
                     PGPVPVTATNKPPGTEVTNILRDVDQIRYIAPADVGRLRAEDFVATVDLTNVKPDGQQ
                     VNVRVAVNAIDPRVTILDSQPRTIPVVLDEVTTKVVPVEVDQGTPPAGIDIGEMTFSP
                     TTVSVTGPASAVSQVVAAHVNVALSPEGIDFDRTIEA"
BASE COUNT          339 a          944 c          887 g          339 t
ORIGIN      
        1 ctggccgcgt cgggcaagct cgccgacgcg gtcgatgccg ctcggcgcgg gctcgaattg
       61 gcggagggtc gcgagcggcg gcggacgctg gagcagctga tcgaacggct ccgcgcgtcg
      121 gagccggccg agcccggccg cgtggcgttg gagcgcgcgc tcgtggtcct cgagggtccg
      181 gcggtcgagg cgcggcgaca gtcgccgccc gcacacgcca agtccacgcc ggccaaggcg
      241 gcggccgctt cgcccgtcga gccgcccgta aagcgccaca aggcgacgcc cgccgtcgag
      301 accgcgcccg aggtcgtcga ggcaaccgag gtcgcagcgg tcgaagcgcc cgcggggtcc
      361 gtacctgcgg tcgcgccccc cgtcgaggag gccgccgggc ccgccgagac cgagcccgcc
      421 ccggaaccgg tgcccgcccc tcgccgggcg ctcgaccgcg acctgccgcc cgacctcgac
      481 ctcgccgagc tcgcgctcgc ggccgaggag acgctggagc gacgcgtcca gggcgtcggc
      541 ctcgaccagc tgctcgatct cgcgagcgcc taccgtcgcg acggccatgc ggaggccgcg
      601 ctcgacacct gctatctcgc cctgtcgatc gcgccggacg acctgagcct ccatctcgcg
      661 ctcgtggagc tgtacgacga gcgcggctgg tccacgctcg cgaccgagaa gctggacctg
      721 ctcgaccggc tcgccgcgct ggatgcggac gagtcgcacc aggcgctcct ggccggcacc
      781 cgggtcgcgc ggggccaggg gggacccccg gcccgatcgt aggggtcccg gaacgggggg
      841 atcccggtgc tagactcgcc agaagatgct cccgttcgtc gaatcgctcg tccgacaggt
      901 cacgctgacg acgctgctcg acatcgcgat caccgcgctg ttcatctact ggttgttcag
      961 cctgatccgg ggaacccggg ccgtcacgct cgtcatcggc gtctcgatcc tgctgggcgt
     1021 ctacgccctc gcccagttcc tccagctgcg gctgttcacc cagatcctcc aggccggcgc
     1081 cgtggtcggc ctgttcgcgc tcgtcgtcgt gttccagccc gagctgcgcc gtggcctcga
     1141 gcggatcggg cgggtcggca ccttcggctg gctcattgcc ccgtcaggtc agcggacgat
     1201 ccagcacgtc gcggccgagg tggcgaaggc cgccggtcag ctgtcgcgtg agggccacgg
     1261 ggccctgatc gtgctggagc gcgagactgg cctcgaggag atcgccgaga ccggtgtgat
     1321 gatccacgcc gacctgtcga gcgagctgtt gtgcacgatc tttgcgcccc gcacgtcgct
     1381 gcacgacggc gcggtcgtca tccgcgacga ccggatcctc gcggccgcgg cgctcctgcc
     1441 gctcaccgag atgacgctgt ccgagcggtt cgggacccgg catcgggccg ccctgggcat
     1501 caccgaggac acggacgccg tcgcggtcgt ggtgtccgag gagagcgggc agatgagcgt
     1561 cgtcgagcgg gcgcgcatcg tgcgcgtccc atccgagacg cagttggagc gggctctcac
     1621 cgccctgctc gaggcgcccg ccacgccgcc cgggctcagc cgtcgccggg gatcggtgtt
     1681 ccgcggacga acccgtgggc gattgcgcat ccggacgacg cccggacgcg tcgatgcgcc
     1741 gccggcgaca ccgggcacgc ccgcgggcgg tcagcccgcc gcgtccggcg gctccgggct
     1801 cagctcggcg gcgatgctgc cgacgaacgg cggaccggcc cccgatccgg gtccgatccc
     1861 tgagacgccc gtcggtgacg agaacgggag cggcgcggcg gcggccgtgg ccggccgatg
     1921 agcctcggct cacgcctgca acgggccgtc cggttcgtcg tccacaactg gccactcaaa
     1981 ctcgccgcga tcgccctggc gacgctgctg tacgcgggcc tcgtcgcctc ccaggacagc
     2041 atcacgtttc ccggcccggt tcccgtcacg gccaccaaca agccgcccgg aacggaggtc
     2101 acgaacatcc tccgcgacgt cgaccagatc cggtacatcg cgccggcgga cgtgggccgg
     2161 ctgcgcgccg aggacttcgt ggcgaccgtc gacctgacca acgtcaagcc cgacgggcag
     2221 caggtcaacg tccgggtcgc cgtcaacgcc atcgacccgc gggtgacgat cctcgactcc
     2281 cagccgcgca cgatcccggt cgtgctcgac gaagtgacga cgaaggtcgt cccggtcgag
     2341 gtcgatcagg gcacgccacc ggcgggcatc gacattggcg agatgacgtt ctcgcccacc
     2401 accgtgagcg tgaccggccc cgcatcggcc gtcagccagg tcgtcgcggc gcatgtgaac
     2461 gtcgccctgt ccccggaggg catcgacttc gaccgcacca tcgaggcga
//
LOCUS       DBNHXO010000334         5873 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4858660, whole genome shotgun sequence.
ACCESSION   DBNHXO010000334 DBNHXO010000000
VERSION     DBNHXO010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5873)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4858660"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="AC162A_06965"
     CDS             complement(<1..1111)
                     /locus_tag="AC162A_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HIR5010619.1"
                     /translation="MALPVPNLDDRRFQDLVDDAKRQVQQRCPEWTDHNVSDPGVTLI
                     ELFAWMTEQVVYRLNRVPDRNYVKFLELIGVSLYPPTAARSPVTFWLSAPQPDVVTIP
                     TGTQVATVRTDTDEAIAFTSIEDLRIVPAELATLGSMIDGKTLRDHMAALEKGSEVFC
                     FQKVPSPGDALYIGLTEAVPSNAVRLRFKADIDGVGVDPTNPPLAWEAWTGDDWEPCE
                     LDSDSTGGLNRDGDVVIHVPRGHVVSVITKQRAGWLRARVTELAEGQPAYSASPNIKG
                     LNAITIGGTVEVVNAELVREEELGVSEDVPGQHFGLKRGPVVPGDEDPVLEVSGDEGW
                     EEWTYVSDFAASGPDDRQFTLDLSSGEVRFGPAVRL"
     gene            complement(1114..1512)
                     /locus_tag="AC162A_06970"
     CDS             complement(1114..1512)
                     /locus_tag="AC162A_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HIR5010620.1"
                     /translation="MSDEFIGRGWAFPLRTDATGGIALVTREREIEEAIRLVLGTAPG
                     ERPMRPEFGCRIHEYVFASADGATAAAIAADVKDALARWEPRIDVDDVGVSFDSRDPT
                     VMYIDIRYAIRRTNDRRNLVFPFYVIPGGD"
     gene            complement(1524..3185)
                     /locus_tag="AC162A_06975"
     CDS             complement(1524..3185)
                     /locus_tag="AC162A_06975"
                     /inference="COORDINATES: protein motif:HMM:NF033848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VgrG-related protein"
                     /protein_id="HIR5010621.1"
                     /translation="MPDPEVSIGRLIEIDGSKLADDIDGQLESATVVDRLAMPDMFAL
                     VFRDPGKDVLGRAGLEVGTKVKISTTSANEDMPKALIDAEVTSIESEYGSHGTLAVVR
                     GYDRSHRLAAGRKTATYLNTKYSDIASQVAGAASLTADVDESEGTFDHVFQINQSDLD
                     FLYGLARQIGYDCRVDGEKLLFKKPVESSSAPAEATGTSASATELVWGDNLLDFRARL
                     SAVAQVSKVEVRGWDPKNKQEVIGTAEATATNAELPTTPGDLAGKIGGQTLVVVDHGV
                     NNQQAADQLATARAEQIGSAAFEATAVAAGSPDLKAGVAVSIGGVDPALAGKWVVSGS
                     RHEFGDGMYRTSLEFTGRQDRSLHGLLSHGGAADRINGVAVAIVSDNDDPEQAGRVRV
                     SLPWLSADAVSDWARIAAPGAGKDSGVVWMPEVGDEVLVAFELGDLSRPIVVGGLWNG
                     KDTIPFDYGSDLDAGSVTYRGFKSRTGHKISFRESSSDSSIELVTQGGAVTITLDDMN
                     KQIKIETEGKLTIDVKQDVEIKAGGSMKLEATGQMTIKGATVAIN"
     gene            complement(3194..3925)
                     /locus_tag="AC162A_06980"
     CDS             complement(3194..3925)
                     /locus_tag="AC162A_06980"
                     /inference="COORDINATES: protein motif:HMM:NF039279.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIS tube protein"
                     /protein_id="HIR5010622.1"
                     /translation="MDAVGSAASAVGQALGLIKGNKAKLVCVGKNMPDGPHEIECSFN
                     PTDYTLSQQVSVPSATTPSREGGQQQYGGTGAMSFTTNLFFDAFSEAKGDVTPKITTL
                     LSWMKPTKSSGSDGRHCPPMVQFKWGGNPQLDKLIGFLTQATVKYTLFRMDGTPVRAE
                     VAITITAQSDPATGTNPTSHAVNSRRVHQVIDGDTLQSVAYRELGKPTYWRSIAELND
                     IDDPMRLPAGTILLIPSVADATRGA"
     gene            complement(3941..4426)
                     /locus_tag="AC162A_06985"
     CDS             complement(3941..4426)
                     /locus_tag="AC162A_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098015 - virus tail [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIR5010623.1"
                     /translation="MGDPAVSVAYKCTIDGFIPLGIWTKIEGLGFEYEIKEFREGGVN
                     GYTHKVLGPCKYTNLRLSRPVDSTSELLMLWLQSNMIKVIPQTMSITAMTMAGDDITT
                     WNLAGVVPVKWSGPSLDIMSNNVATETLEVTYQEIIGLGALGGLLAGAMSMSANVSVG
                     F"
     gene            complement(4439..5350)
                     /locus_tag="AC162A_06990"
     CDS             complement(4439..5350)
                     /locus_tag="AC162A_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010624.1"
                     /translation="MLTVARQRRDASTSGPTTPASPTSVARATPTTGAPTVSRSASTV
                     LPTVGVRPLRPSVGASATVGPDRPAAGTAGAAATNADAPAPVLARWDSGDALPATVTS
                     LSASPTADRVLPVQLSAIQDAPAGAAMQRPSTPAPREIVFPAPDFGGQPTVGDAGGWP
                     VPTTLPAQPATTAPRPASPARPASTPLSLARTVAAAPTPAPAPTPAAAMPVVARIVAD
                     PASPGTPPVVQTSSAGGGIPVATFTATPIVQREEAPAPGATPERAGRSDRELDELAKQ
                     LFGRIRGQLKAEVIHEREAKGLTFDSF"
BASE COUNT          794 a         1997 c         2162 g          920 t
ORIGIN      
        1 cgagccgcac cgccgggccg aatcggacct cgcccgacga caggtcgagc gtgaactgcc
       61 ggtcatcggg cccactggcg gcgaagtcgg acacgtaggt ccactcctcc cagccctcgt
      121 cgcccgagac ctcgaggacc gggtcctcgt cgcccgggac caccgggccg cgcttgaggc
      181 cgaagtgctg gccgggaacg tcctcggaga cgcccagctc ctcctcgcga acgagctcgg
      241 cgttgacgac ctcgacggtg ccgccgatcg tgatcgcgtt gaggcccttg atgttcgggg
      301 acgcgctgta cgccggctgc ccctcggcga gctcggtgac gcgggcccgg agccagccgg
      361 cgcgttgctt ggtgatcacc gagacgacgt gaccacgcgg gacgtggatc accacgtcac
      421 cgtcgcggtt caggccgccg gtcgagtccg agtcgagctc gcacggctcc cagtcgtcgc
      481 ccgtccaggc ctcccaggcg agcggcgggt tggtgggatc gacgccgacg ccgtcgatgt
      541 cggccttgaa gcgcaggcga acggcgttcg aggggaccgc ctcggtgaga ccgatataca
      601 gggcgtcgcc gggcgacggc accttctgga agcagaagac ctcggagccc ttctcgagcg
      661 cggccatgtg gtcgcggagc gtcttgccat cgatcatcga gcccagcgtc gccagctcgg
      721 ccgggacgat ccgcaggtct tcgatcgagg tgaacgcgat cgcttcgtcc gtgtcggtcc
      781 ggacggtggc cacctgggtg ccggtcggga tcgtcacgac gtccggctgt ggcgcggaca
      841 gccagaatgt gaccgggctg cgggccgccg tcggcgggta gaggctgacg ccgatgagct
      901 cgaggaactt gacgtagttc cggtccggga cgcggttcag gcgatagacg acctgctcgg
      961 tcatccaggc gaacagctcg atcaacgtca cgcccgggtc agagacgttg tgatcggtcc
     1021 actcggggca gcgctgctga acctggcgct tggcatcgtc gacgaggtcc tggaagcggc
     1081 gatcgtcgag gttgggaacc ggcagggcca tggtcagtct cctcccggga tcacgtagaa
     1141 cgggaatacg aggttgcggc ggtcgttggt ccgccggatg gcgtagcgga tgtcgatgta
     1201 catcaccgtc gggtcacgcg agtcgaagct cacgcccacg tcgtcgacgt cgatccgggg
     1261 ctcccagcgg gccagcgcgt ccttgacgtc ggcggcgatc gcggccgcgg tcgcgccatc
     1321 ggccgaggcg aacacgtact cgtggatccg gcagccgaac tccgggcgca tcgggcgctc
     1381 gccgggcgct gtccccagca ccagccggat ggcctcctcg atctcgcgtt cgcgcgtgac
     1441 cagcgcgatc ccgccggtcg cgtctgtccg cagcgggaac gcccagcctc gtccgatgaa
     1501 ctcgtcgctc acgcgcgttt ctcctagttg atggcgacgg tcgcgccctt gatcgtcatc
     1561 tgacccgtgg cctcgagctt catcgagccg ccggccttga tctccacgtc ctgcttgacg
     1621 tcgatcgtca acttgccctc ggtctcgatc ttgatctgct tgttcatgtc gtcgagcgtg
     1681 atcgtcacgg cgccgccctg ggtcaccagc tcgatcgagg agtcgctgct gctctcccgg
     1741 aagctgatct tgtggccggt gcgcgacttg aacccgcgat acgtgacgga gccggcgtcg
     1801 aggtcagatc catagtcgaa cgggatcgtg tccttgccgt tccacagccc accgaccacg
     1861 atcggccggc tgagatcgcc aagctcgaac gcgacgagca cctcatcgcc gacctcgggc
     1921 atccagacca cgcccgagtc cttgccggcg ccgggcgcgg cgatccgcgc ccagtccgag
     1981 acggcgtcgg cggacagcca cgggagggac actcgcacgc gccctgcctg ctcgggatcg
     2041 tcgttgtcgc tgacgatggc gacggcgacg ccgttgatcc ggtcggcggc gccaccgtgc
     2101 gacagcaggc cgtggagcga tcggtcctgg cgccccgtga actcgagcga cgtgcggtac
     2161 atgccgtcgc cgaactcgtg gcgcgacccg gagacgaccc atttcccggc cagcgccggg
     2221 tcgaccccgc cgatgctgac cgcgaccccg gccttgaggt ccggcgaccc ggcagcgacc
     2281 gcggtcgcct cgaaggccgc cgacccgatc tgctcggcgc gggccgtcgc aagctggtcg
     2341 gccgcctgct ggttgttgac gccgtgatcg acgacgacca gcgtctggcc accgatcttg
     2401 ccggccaggt ccccgggcgt cgtcggcagc tccgcgttgg tcgccgtcgc ctcggccgtc
     2461 ccgatgacct cctgcttgtt cttcgggtcc cagccgcgga cctccacctt gctgacctgc
     2521 gccaccgcgc tcaagcgcgc ccggaagtcg agcaggttgt cgccccatac gagctcggtc
     2581 gcgctcgcgc tggtcccggt cgcctcggcc ggcgcgctcg aggactcgac cggcttcttg
     2641 aacagcagct tctccccgtc gacccggcag tcgtagccga tctggcgcgc gaggccgtac
     2701 aggaagtcga ggtcggactg gttgatctgg aagacgtggt cgaacgtccc ctcggactcg
     2761 tcgacgtcgg cggtcaggct cgcggcgccg gcgacctggg acgcgatgtc cgagtacttc
     2821 gtgttcaggt aggtcgcggt cttgcgcccg gccgccaacc ggtgcgagcg gtcgtagcca
     2881 cggacgacgg cgagcgtgcc gtgggacccg tactcgctct cgatcgacgt cacctcggcg
     2941 tcgatgagcg ccttgggcat gtcctcgttc gccgacgtcg tcgagatctt gaccttggtg
     3001 ccgacctcga gcccggcccg cccgaggacg tccttgcccg gatcccggaa cacgagcgcg
     3061 aacatgtccg gcatcgccag gcggtcgacg acggtcgcgc tctcgagctg gccgtcgatg
     3121 tcgtccgcta gcttcgaccc atcgatctcg atgaggcggc cgatggagac ctcgggatcg
     3181 ggcatggcgt gattcaggcg ccacgcgtcg cgtcggcgac gctagggatg agcaggatcg
     3241 tgcccgccgg caggcgcatc gggtcgtcga tgtcgttgag ctcggcgatc gagcgccaat
     3301 aggtgggctt gccgagctcg cggtaggcga cggactggag cgtgtcgccg tcgatcacct
     3361 ggtgcacgcg ccggctgttc acggcgtgcg acgtggggtt ggtgccggtc gccggatcgg
     3421 actgggcggt gatggtgatg gcgacctcgg cccgcacggg cgtgccgtcc atgcggaaca
     3481 gcgtgtactt gaccgtcgcc tgggtcagga acccgatgag cttgtcgagc tgcgggttgc
     3541 cgccccactt gaactggacc atcggcgggc agtggcggcc gtccgagccc gacgacttgg
     3601 tcggcttcat ccaggacaac agcgtcgtga tcttgggcgt cacgtcgccc ttggcctcgg
     3661 agaaggcgtc gaagaacagg ttcgtcgtga acgacatcgc cccggtgccg ccgtactgct
     3721 gctggccgcc ctcacggctg ggcgtcgtgg cgctcgggac tgacacctgt tgggacagcg
     3781 tgtagtcggt cggattgaac gagcactcga tctcgtgcgg gccgtcgggc atgttcttgc
     3841 ccacgcacac gagcttggcc ttgttgccct tgatcagccc gagggcctgt ccgaccgccg
     3901 atgccgccga tccgaccgcg tccatcacca tggccggccc ctagaacccc acgctcacgt
     3961 tggccgacat ggacatcgcg ccagccagca gcccgccgag tgcgccgagc ccgatgatct
     4021 cctggtacgt gacctcgagc gtctcggtcg cgacgttgtt gctcatgatg tcgaggctgg
     4081 gcccgctcca cttcacgggc accacgccgg ccaggttcca ggtggtgatg tcgtcgccgg
     4141 ccatggtcat cgcggtgatc gacatcgtct gggggatgac cttgatcatg ttgctctgga
     4201 gccagagcat cagaagctcg gacgtcgagt cgaccggccg gctcaggcgc aggttcgtgt
     4261 acttgcacgg cccgaggacc ttgtgcgtgt acccgttgac cccgccctcg cggaactcct
     4321 tgatctcgta ctcgaacccc aggccctcga tcttggtcca gattcccagg gggatgaagc
     4381 cgtcgatcgt gcacttgtac gcgacgctga ctgccggatc gcccatcggt cctccctgct
     4441 agaacgaatc gaacgtgagg cccttggcct cgcgctcgtg gatgacttcc gccttgagct
     4501 ggccccggat gcgcccgaac agctgcttgg ccagctcgtc cagctcccgg tcggaccggc
     4561 cggcacgctc gggcgtggcg cctggggccg gggcctcctc gcgctggacg atgggcgtcg
     4621 cggtgaaggt ggcgacgggg ataccgccgc cggcagacga tgtctggacg acgggcgggg
     4681 tgccgggcga cgcaggatcg gcgacgatcc gggccacgac cggcatggcg gccgcgggcg
     4741 tgggggccgg cgcgggtgtc ggtgcagcgg cgacggtccg cgccagcgag agtggcgtcg
     4801 atgccggtcg agcaggggag gccggccggg gggcggtcgt cgccggctgg gctgggaggg
     4861 tcgtcggcac cggccagcca ccagcgtcgc cgaccgtcgg ctggccgccg aagtcggggg
     4921 cggggaagac gatctcgcgc ggcgcgggcg tggatggccg ttgcatcgcc gcgccagcgg
     4981 gcgcgtcctg gatcgccgac agctggaccg gcagcacgcg atcggcggtc ggcgatgccg
     5041 agagggaagt caccgtagcg ggaagcgcgt cgcccgagtc ccagcgcgcg aggaccggcg
     5101 ccggggcgtc ggcgttggtc gcggcggcgc cggccgtgcc cgccgccggg cgatcaggcc
     5161 cgaccgtggc ggaggcaccg accgagggac ggaggggccg gacgccgacc gtcggcagga
     5221 ccgtggacgc cgagcgggag acggttggcg cgccggtggt cggggtggcc cgggcgaccg
     5281 acgtcggtga cgccggggtg gtcggaccgg aggtcgaggc gtcacgccgt tgacgggcga
     5341 ccgtgagcac cggaagcggg cgcggggcga tcggcgccgc ggggccgtga tcgtggccgc
     5401 ggtgcgggtc ggcggccccc ggggcggcgg ttgaggcctc cgccggaccg tggccgccgg
     5461 acggctcgga cgctgcagcc ggttcgaccg gcaggcgctg cgccgtggcg cttgccggcg
     5521 gcacagtgat cggggcgccc aggcccgcgc ggcggggcgt ggcgccggcc tgacgctggg
     5581 cggcgggccg cggcgtctcg acgggcagct ccgcgaaccg acgaatcggc gcctgcgcgg
     5641 gtctcgcggg atcgggtacg ccggtcggcg aggacgcgga cgcggggatc cggccgctcg
     5701 cctggatggg caaagcgagg ttcgcgggcc gcgacccgcg gccgggatcc gtgggcgagc
     5761 cggacgcgcg cgacgagctc acggggagcg gcgccagcgt cggcgaggcc tgggtgagcg
     5821 gcctcgggct cggcgtgacg gtcgcggccg gagttaccgg cgagaccgtc cgg
//
LOCUS       DBNHXO010000335         2553 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1486006, whole genome shotgun sequence.
ACCESSION   DBNHXO010000335 DBNHXO010000000
VERSION     DBNHXO010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1486006"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="AC162A_06995"
     CDS             <1..782
                     /locus_tag="AC162A_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005561297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase-like TK C-terminal-containing
                     protein"
                     /protein_id="HIR5010625.1"
                     /translation="CATFLTFSDYMRGSVRLASLSGLHVTYVWTHDSVGLGEDGPTHQ
                     PVEHYAALRAIPNLWFVRPGDANEAAAAWALAVERPTIEGAGRGPVALAFTRQKLPTL
                     AGSPEKAREGLRRGGYVLREASGGAPQLILIGTGSELQLAFAAAEALEADGIPTRVVS
                     LPCWERFEAQDQAYLDAVLPPAVKKRVSVESGVSLGWERWVGDEGAMIGIDHFGASAP
                     AGTIFKEFGFTANRVADVGRRVVRDGLHGRQPTLGSPHPGH"
     gene            787..1251
                     /locus_tag="AC162A_07000"
     CDS             787..1251
                     /locus_tag="AC162A_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HIR5010626.1"
                     /translation="MRVAFAADHAGVAFKDELIHRLVVALGGQHELTDLGGDGSDPND
                     DYPDFAQAVGEAVRDGRADRGILICGSGVGASVAANKIPGIRAAICHDTYSAHQGVEH
                     DDMNVLTLGARVIGIEPAVECSLAFLAATFSGEPRHQRRLDKVLAIEAAARD"
     gene            1315..2157
                     /locus_tag="AC162A_07005"
     CDS             1315..2157
                     /locus_tag="AC162A_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010627.1"
                     /translation="MTDAAVATGAPATAIDERSVTSPGGEVLRDIARGGFAGILVGYV
                     VAGLGGRLVMRLATILHEDTVGQTTEAGETIGRITLNGTLALMFFSGLGMGLVAGTVW
                     VIVSPWIPWRRLTRAFVTAVAAIAIGTPSLVQFTNFDFVILDHDPLVIAMLIGLVGLV
                     GFSIALVDDVLEARLPHPRRGIGRSTIAYLIVTLLGLVVILPVVVAILLGGPEYNAPI
                     RAGWALAITGVCTLAWWVLRVRGESTPPRVLRLIGSGSLVAAVILGFVTCLPQVTGAL
                     GLPR"
     gene            2190..>2553
                     /locus_tag="AC162A_07010"
     CDS             2190..>2553
                     /locus_tag="AC162A_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HIR5010628.1"
                     /translation="MAQFDLPLDELRAYRPPRDEPADFDVFWSETLAESRAVRAPAVA
                     VDVDSHLETISAADVTFSGYAGQPIKAWLLWPAGRTGPLPAIVEYIGYGGGRGRPVDW
                     LVYASAGYAHLVMDTRGQG"
BASE COUNT          322 a          902 c          932 g          397 t
ORIGIN      
        1 actgcgcgac gttcctgacc ttcagcgact acatgcgcgg ctccgtgcgg ctggcgtcgc
       61 tgtccgggct gcacgtgacg tatgtctgga cgcacgactc ggtgggcctc ggcgaggacg
      121 ggccgaccca ccagccggtc gagcactatg cggcgctgcg cgcgatcccg aacctgtggt
      181 tcgtccgccc gggcgacgcg aacgaagccg cggcggcgtg ggcgctggcg gtcgaacggc
      241 cgacgatcga gggcgcgggg cggggtccgg tcgcgctggc gttcacccgc cagaagctgc
      301 cgacgctggc cgggtcgccg gagaaggcgc gggaaggcct gcggcgcggg ggctacgtgc
      361 tccgcgaggc gtcgggcggt gcgccgcagc tgatcctgat cggcaccggc tccgagcttc
      421 agctggcctt cgcggccgcc gaggcgctcg aggccgatgg gatcccgacg cgagtcgtga
      481 gcctgccctg ctgggagcgg ttcgaggccc aggaccaggc ttacctggac gcggtcctgc
      541 cgccggccgt gaagaagcgg gtctctgtcg agtccggcgt ctcgctcggc tgggagcggt
      601 gggtcggcga cgagggggcg atgatcggca tcgaccactt cggcgcgagc gcccccgcgg
      661 gcacgatctt caaggagttc ggctttaccg ccaatcgggt tgcggacgtc gggcggcgcg
      721 tggttcgcga cggcctccac ggacgccagc cgacgctggg ttcgccccac ccggggcact
      781 gatccgatgc gcgtcgcgtt cgccgccgat cacgccgggg tcgcattcaa ggacgagctc
      841 atccatcgcc tcgtcgtcgc gctgggtggc cagcacgagc tcacggacct cggcggcgac
      901 ggctccgatc ccaacgacga ctaccccgac ttcgcccagg ccgtgggtga ggcggtgcgc
      961 gacggtcgtg ccgaccgcgg gatcctgatc tgcggctccg gcgtgggcgc gtccgtggcg
     1021 gccaacaaga tcccgggcat ccgggcggcg atctgccacg acacgtactc ggcccaccag
     1081 ggggtcgagc acgacgacat gaacgtgctg acgctcggcg cccgcgtcat cggaatcgag
     1141 cccgcggtcg agtgctcgct cgcgttcctc gcggcgacgt tctccggcga gccgcgccac
     1201 cagcgccgcc tggacaaggt gctcgcgatc gaggccgccg cgcgcgattg acacgcggcc
     1261 gcgggccgcg tatatagcgt gtgacgatat agcgacccac ggctgaggcg cgccatgaca
     1321 gacgcggcag tggcgacggg ggcgccggca acggcgatcg acgagcggtc cgtgacttcg
     1381 cccggcggcg aggtcctgcg cgacatcgcc cggggtggct tcgcgggcat cctcgtcggg
     1441 tacgtcgtcg cgggcctcgg tggccggctg gtcatgcggc tggcgacgat tctccacgag
     1501 gacacggtcg gccagacgac cgaggcgggc gagaccatcg ggcgcatcac gctcaacggg
     1561 accctggccc tgatgttctt cagcggccta ggcatgggcc tcgtcgccgg aaccgtatgg
     1621 gtcatcgtca gcccatggat cccatggcgt cggctcaccc gcgccttcgt gacggccgta
     1681 gcagcgatcg cgatcgggac gccgtcgctc gtccagttca cgaacttcga cttcgtgatc
     1741 ctcgaccacg acccgctcgt catcgccatg ctcatcgggc tcgtgggcct cgtcggcttc
     1801 tcgatcgcgc tcgtcgacga cgtcctcgag gcgcggctcc cgcatccgcg tcgcgggatc
     1861 gggcgctcga ccatcgccta cctgatcgtg acgctgctgg gcctcgtcgt gatcctcccc
     1921 gtcgtcgtcg ccatcctgct gggcggcccc gagtacaacg cgccgatccg ggccggctgg
     1981 gcgctggcga tcacgggggt gtgcacgctc gcctggtggg tgctgcgcgt gcgcggtgaa
     2041 tcgacgccgc caagggtgct gcggctgatc gggtcgggat cgctggtggc cgccgtgatc
     2101 ctgggcttcg tgacgtgcct gccccaggtc accggcgcgc tgggtctgcc tcgctgaatt
     2161 cggcgcgctg atccggctac gatccgcgca tggcccagtt cgacctcccc ctcgacgagc
     2221 tccgcgcgta ccgtcccccg cgcgacgagc ccgcggactt cgatgtcttc tggtcggaaa
     2281 ccctcgcgga gagccgcgcc gtccgggctc cggcggtggc ggtcgacgtc gactcgcatc
     2341 tcgagaccat cagcgcggcc gacgtcacgt tctcgggcta cgccggccag ccgatcaagg
     2401 cctggctcct gtggcccgcc gggcgaacgg ggccgctgcc ggccatcgtc gagtacatcg
     2461 gctacggcgg gggtcgcggc cggccggtcg actggctcgt ctatgcctcg gccggctacg
     2521 cgcacctcgt gatggacacc cgggggcagg gcg
//
LOCUS       DBNHXO010000336         4993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4063559, whole genome shotgun sequence.
ACCESSION   DBNHXO010000336 DBNHXO010000000
VERSION     DBNHXO010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4063559"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="AC162A_07015"
     CDS             <1..235
                     /locus_tag="AC162A_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010629.1"
                     /translation="ATTTRTPMDQLRSHEVQYRIEHRHHDGSWGEMAEQRQPHDPADT
                     DVERLWGLRRLFRCTSCDESVIVEPGDGTGQPQ"
     gene            complement(223..1659)
                     /locus_tag="AC162A_07020"
     CDS             complement(223..1659)
                     /locus_tag="AC162A_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5010630.1"
                     /translation="MPQEPAPSALPSAAIDASAWSSAQDGVVEFLRDLIRIPSINPPD
                     PPGAELAAAVRIADELGLAGLAPEVVEAVPGRGSVVARLRGDGTGGEPLLLLSHLDVV
                     PAIAEDWTHPPFAGDLADGYVWGRGAVDMKAMVALEVGVLRLLAGRARAAGLDPAVDP
                     VPGLRRDVLFACTADEEAGGWNGAGWLVDNRPETIRASGALNEAGGIPIEIGGRRFYA
                     IGVAEKGFEVYRIRVRGTWGHGSMPRDDNALVLAAEVVTRIAVKGEPRPTPVLRRLLA
                     EVAAALEPDQARLMDAILDDDPRRSEAAIRAACDPVYALALSALLRDTFSPTIMHAGI
                     KYNVIPGEAVIEVDVRKLPGTTEGDVREELLARIGDLAPRCEIEHVIGAVSVEAPAEG
                     ALYEAMVAAIRAHDPDGIPVPVIAPFATDAKHTARLGVPTYGFSPLRLGTGERFLDRF
                     HAVDERVGVEALRWGLPVLYDVVAGYCG"
     gene            complement(1662..1928)
                     /locus_tag="AC162A_07025"
     CDS             complement(1662..1928)
                     /locus_tag="AC162A_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010631.1"
                     /translation="MTAEPAPTPLPPDPVQYDAVRNEHARKRGLPGPYIAGGTDPELP
                     ETLARERPYVRLLIAMVIAIVVIGFLLGVVEALLAVPPLDVPPI"
     gene            complement(1971..3020)
                     /locus_tag="AC162A_07030"
     CDS             complement(1971..3020)
                     /locus_tag="AC162A_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR5010632.1"
                     /translation="MGVVDLNGIRLRNRLVTSASLLGYGASKRRLILYGLSPIAQWVP
                     LERFGAVTTRTMTLEPREGHFTLREDWGVSEFPAMLRLYGKALRRVDAGWLNAFGWSN
                     IGIERYFDEYFGRTADLNRIVSIGGFSADEIVQLVETVNERAKPGEIAAIELNASCHN
                     VNFPFETILEESLARAVPRSRHPVILKVSPDEDYGWQARLAEQHGCAGITAINTVKGL
                     RLDPDTGEPWLKNRYGAVSGRAIKPIGLRVVAELRDAGIRLPIIANGGIRDFDDCREY
                     FWAGADAVSLGSAVWLQRMPLYALGPVEGLRIRRLISRVAGFTPPDAAPHWRPAVDAP
                     STRTDAIAETPVAAT"
     gene            complement(3057..4025)
                     /locus_tag="AC162A_07035"
     CDS             complement(3057..4025)
                     /locus_tag="AC162A_07035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5010633.1"
                     /translation="MTEGGAGDGGPEVWRLEAGDEDGLRAYVDIVNTVAPESPTSLED
                     IAWSDATYPGGARFLGSLDGRPVGTASVGRIYMYEASFERYWFDVHVLPAARRRGLGT
                     RLLVACSRVARDAGKTGLQTDVSERHADGLAFLAGHGFEVIERAKMVQLDLRGLAPPA
                     ADPPPGFAFTTLAERPDLEHGVYAIAVAAYLDIPSVDAPVAVGTMEEFVARDVRRDGI
                     PADGFFIALDEASGEVAGWASLMFVPGSTTMAWHDMTAVGRAWRGRGIATALKRATIA
                     WSIEHGLEILETGNDEDNGPMRAVNIKLGYLPIPDLLTMRGPLAPR"
     gene            complement(4022..>4993)
                     /locus_tag="AC162A_07040"
     CDS             complement(4022..>4993)
                     /locus_tag="AC162A_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR5010634.1"
                     /translation="IADAGGARPEHRRLAPMASTRPGLVGFRISATESTGLDWPRVDE
                     TWALAGELGVFDAGWMSDHVSDAARERNGPAMESLTTVSALAHRVPGKWIGIAVAANT
                     FRHPALLAKSATVLDNVTGGRFILGLGAGWHAGEHEAFGIPLPPLPERYDRFESALRV
                     LTALFSDAAHRAPGVSLDDPHFPLRGATNEPGAVRPGGPAIWLGGQKRRGIELAARYA
                     EGWPMPGNRPGDVDYFALKRDEISRALEAAGRDPADFTFAAQLSCGATADSRRDALET
                     ARAFLAVGANHVILGVPASMAPDGLAVVADEVAQPLAERAAAPSPLA"
BASE COUNT          692 a         1807 c         1854 g          640 t
ORIGIN      
        1 agccacaacg acgaggacgc cgatggacca gctccgctcc cacgaggtgc agtaccggat
       61 cgagcaccgc caccacgatg gcagctgggg cgagatggcc gagcagcgcc agccccatga
      121 tccggccgac accgacgtcg agcgcttgtg ggggttgcgc aggctgttcc gctgcacgtc
      181 ctgcgatgag agcgtgatcg tcgagcctgg cgacgggacc ggtcagccgc agtagccggc
      241 gaccacgtcg tagaggaccg gcaggcccca gcgcagggcc tcgacgccga cccgttcgtc
      301 gaccgcgtgg aaccggtcga ggaaccgctc cccggtccca agccggagcg gcgagaaccc
      361 gtaggtaggg acgcccagcc ttgcggtgtg cttggcgtcg gtggcgaacg gcgcgatcac
      421 cgggaccggg atgccgtcgg ggtcgtgggc gcgaatggcc gcgaccatcg cctcgtacag
      481 ggcgccctcg gccggcgcct cgaccgatac cgcgccgatc acgtgctcga tctcgcagcg
      541 cggcgcgagg tcgccgatgc gcgccagcag ctcctcgcgg acgtcgccct cggtcgtgcc
      601 cgggagcttg cgcacgtcga cctcgatcac cgcctcgccg gggatcacgt tgtacttgat
      661 gcccgcgtgc atgatcgtgg ggctgaacgt gtcccgcagc agcgcgctca gggccagcgc
      721 gtagaccggg tcgcaggcgg cgcggatcgc ggcctcggag cggcgggggt cgtcgtcaag
      781 gatcgcgtcc atgagcctcg cctggtcggg ttcgagcgcc gccgccacct cggcgagcag
      841 ccggcgcagg accggcgtcg gacgtggctc gcccttgacg gcgatgcggg tcacgacctc
      901 ggcggcgagg acgagcgcgt tgtcgtcgcg gggcatcgag ccgtggcccc aggtgccccg
      961 cacccggatg cggtagacct cgaagccctt ctcggcgacc ccgatcgcgt agaaccgccg
     1021 gccgccgatc tcgatcggga tgcccccggc ctcgttcagt gcgccggacg cgcggatcgt
     1081 ctcgggccgg ttgtcgacca gccagccggc gccgttccag ccgcccgcct cctcgtcggc
     1141 ggtgcaggcg aacaggacgt cgcgccggag gccgggcacc gggtcgacgg ccgggtccag
     1201 gcccgccgcc cgtgctcgcc cggcgagcag ccgcagcacg ccgacctcga gcgccaccat
     1261 cgccttcatg tcgactgcgc cccggcccca gacgtacccg tcggcgaggt cgcccgcgaa
     1321 cgggggatgc gtccagtctt ccgcgatcgc cgggacgacg tcgaggtgcg acaggaggag
     1381 cagcggctcg ccgccggtgc cgtcgccgcg cagccgcgcc accacgctgc cgcgacccgg
     1441 gacggcctcg acgacctcgg gcgcaagacc ggccaggccc agctcgtccg cgatccgcac
     1501 ggcggccgcg agctcggcgc ccggcgggtc cggcgggttg atcgacggga tccggatcag
     1561 gtcgcgcagg aactcgacca cgccgtcctg ggccgaggac caggcggacg cgtcgatcgc
     1621 cgccgacggc agggctgacg gcgccggctc ctggggcatg atcagatcgg cgggacgtcg
     1681 agcggcggca ccgcgagcag cgcctcgacg acgccgagca ggaacccgat cacgacgatc
     1741 gcgatcacca tcgcgatcag caggcgcacg taggggcgct cgcgcgcgag ggtctcgggc
     1801 agctcggggt ccgtgccgcc ggcgatgtac ggcccgggca gcccgcgctt gcgggcgtgc
     1861 tcgttgcgga cggcgtcgta ctggaccggg tcggggggca gcggcgtcgg ggcgggctcg
     1921 gcggtcaccc gcccatcatg ccgcgcggcg gccaaccgtg ccggtatcgg tcacgtggcg
     1981 gcgaccggcg tttccgcgat ggcgtcggtc cgggtcgacg gggcatccac ggccggacgc
     2041 cagtgcgggg ccgcgtcggg tggcgtgaac ccggcgaccc gcgagatcag gcggcggatc
     2101 cgcaggccct cgaccgggcc cagcgcgtac agcggcatgc gctggagcca gacggcggag
     2161 ccgaggctca ccgcgtcggc gccggcccag aagtactcgc ggcagtcgtc gaagtcgcgg
     2221 atcccgccgt tggcgatgat tggcagccgg atcccggcgt cgcgcagctc ggcgacgacg
     2281 cgcagcccga tcggcttgat cgcccgccct gagaccgctc cgtagcggtt cttgagccac
     2341 ggctcgccgg tgtcggggtc caggcgcagg cccttcacgg tgttgatcgc cgtgatcccc
     2401 gcgcacccgt gctgctcggc caggcgcgcc tgccagccat agtcctcgtc gggcgagacc
     2461 ttgaggatca cggggtgacg gctgcgcggg acggcgcggg cgaggctctc ctcgaggatc
     2521 gtctcgaacg ggaagttgac gttgtggcac gaggcgttga gctcgatggc cgcgatctcg
     2581 ccgggcttcg cccgctcgtt gacagtctcg accagctgga cgatctcgtc cgccgagaag
     2641 ccgccgatcg agacgatccg gttgaggtcc gccgtgcggc cgaagtactc gtcgaagtag
     2701 cgctcgatgc cgatgttcga ccacccgaac gcgttgagcc agcccgcgtc gacccgccgc
     2761 agcgccttgc cgtacagccg gagcatggcc gggaattcgc tgacgcccca gtcctcgcgc
     2821 agggtgaagt ggccctcgcg cggctcaagg gtcatggtcc gcgtggtcac ggcgccaaag
     2881 cgctccagcg ggacccactg ggcgatcggg gagaggccgt acaggatcag ccgtcgcttc
     2941 gacgccccgt agcccagcag gctggcggag gtgacgagcc ggttccgcaa ccggatcccg
     3001 ttgaggtcga cgacgcccac gccagaagta tgccgggcct gtcccgggag ggcggctcag
     3061 cggggggcga ggggcccgcg catcgtgagc aggtcgggga tcgggaggta gccgagcttg
     3121 atgttcacgg cgcgcatcgg gccgttgtcc tcgtcgttgc cggtctcgag gatctcgagt
     3181 ccgtgctcga tcgaccacgc gatcgtcgcc cgcttcagcg cggtcgcgat gccacgccct
     3241 cgccacgccc gtccgacggc ggtcatgtcg tgccaggcca tggtcgtcga gcccgggacg
     3301 aacatcagcg acgcccaccc ggcgacctcg ccactggcct cgtcgagggc gatgaagaac
     3361 ccgtcggccg ggatgccgtc acgccggacg tcgcgggcga cgaactcctc catggtgccc
     3421 acggccaccg gcgcgtcgac gctcgggatg tcgagatacg ccgcgaccgc gatcgcatag
     3481 acgccgtgct ccaggtcggg ccgctcggcg agcgtggtga acgcgaatcc cggtggcggg
     3541 tcggcggccg gcggcgcgag gccccgcagg tcgagctgga ccatcttggc gcgctcgatg
     3601 acctcgaacc catgaccggc gaggaacgcc aggccgtcag cgtgccgctc cgacacgtcg
     3661 gtctggagac ccgtcttgcc cgcgtcgcgg gccacccgag agcacgcgac cagcagccgc
     3721 gtcccgagcc cccggcgccg cgccgccggc aggacgtgca cgtcgaacca gtagcgctcg
     3781 aagctggcct cgtacatgta gatccgtccg acagacgcgg ttccgacggg ccggccgtcg
     3841 agcgagccga ggaagcgcgc gccgcccgga taggtcgcgt cggaccaggc gatgtcctcc
     3901 agggacgtcg gcgactctgg ggcgaccgtg ttgacgatgt cgacgtaggc acggaggcca
     3961 tcctcgtcgc cggcctcgag tcgccagacc tccgggccgc cgtcgccggc cccgccctcg
     4021 gtcacgccag cggcgaaggg gcggccgcgc gctccgccag gggctgggcg acctcgtcgg
     4081 cgacgaccgc gagcccgtcg ggtgccatgc tcgcgggaac gcccaggatc acgtggttgg
     4141 cgcccacggc gaggaacgct cgagccgtct cgagcgcgtc gcgacgcgag tccgcggtgg
     4201 cgccgcacga cagctgcgcg gcgaaggtga agtcggcggg atcgcggccg gccgcctcga
     4261 gcgcccgcga gatctcgtcc cgcttgaggg cgaagtagtc gacgtcgccg gggcggttgc
     4321 cgggcatcgg ccagccctcg gcatagcgcg ccgcgagctc gatgccccgg cgtttctggc
     4381 cgcccagcca gatcgccggg ccgcccgggc ggaccgctcc cggctcgttg gtcgcgccgc
     4441 ggagcgggaa gtgcggatcg tcgagcgaca ccccaggcgc acggtgcgcc gcgtcggaga
     4501 acagcgccgt cagcacccgc agcgcgctct cgaaacggtc atagcgctcg ggcagcggcg
     4561 gcagcgggat cccgaaggcc tcgtgctcgc cggcgtgcca gccggcgccg agcccgagga
     4621 tgaaccggcc gccggtcacg ttgtccagca cggtcgcgga ctttgcgagc agggccgggt
     4681 gccgaaacgt attggccgcg accgcgatgc cgatccactt cccgggcacg cggtgcgcga
     4741 gcgccgagac cgtggtcagc gactccatcg ccgggccgtt gcgctcgcgg gccgcgtccg
     4801 agacgtggtc gctcatccag ccggcgtcga acacgccgag ctcgcccgcc agggcccagg
     4861 tctcgtcgac ccgcggccag tcgagcccgg tggactcggt ggccgagatc cggaagccga
     4921 cgaggcccgg ccgcgtactg gccatcggcg ctagccgccg gtgctccggg cgagcgccgc
     4981 ccgcatctgc gat
//
LOCUS       DBNHXO010000337         1685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_889632, whole genome shotgun sequence.
ACCESSION   DBNHXO010000337 DBNHXO010000000
VERSION     DBNHXO010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_889632"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="AC162A_07045"
     CDS             <1..771
                     /locus_tag="AC162A_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010635.1"
                     /translation="DARGEETDHRMTDQDRDPAAEPGADDATEAVSEPEAVPAPDGED
                     APEPAADEPHAEAAPEPEPEPEPEPEPEPEPEPEADEAADDEADEADDDEVDEAGDDE
                     PGAVPAAAAAAAATTTARRAKDRPPTKSAPAAPTVSEQAVHINDRASAIFVIVVVGVF
                     AIIFLNALLLGHKGLLSGLLPQPTPIASEAPSASAGPSASPSGSAAASPSGSPPASPS
                     AAASPSAAASPSAAASPSAEPSPSAASPAAPSPSPAAS"
     gene            782..1450
                     /locus_tag="AC162A_07050"
     CDS             782..1450
                     /locus_tag="AC162A_07050"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR5010636.1"
                     /translation="MDAARARASEARARMVADQLRKRDIRDERVLSAMTAVERDAFVP
                     ERDRDAAYTDSPLPIGGGQTISQPYIVARMTELLAVESGDRVLDVGTGSGYQAAVLAE
                     LGCLVTSIERDPDLALAAMDHLVAAGYGDRVIVRVGDGSLGAPDEAPWDGIVVAAGAP
                     AIPEPLREQLGEGRRLVIPVGGLREQSLMVVERHGDEWTEQSDGPCVFVPLVGAEGWT
                     ASAL"
     gene            1447..>1685
                     /locus_tag="AC162A_07055"
     CDS             1447..>1685
                     /locus_tag="AC162A_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010637.1"
                     /translation="MSLLLGGKSVAPLFRRLGPVFAVAGAVVAAVTWPRAFDLEAYLD
                     AAKLVAAGQDPYAATRALGVAEWGQGQVYVSPPFIA"
BASE COUNT          221 a          633 c          630 g          201 t
ORIGIN      
        1 gacgcccgcg gagaggagac cgatcaccgg atgaccgatc aggaccgcga cccggcagcc
       61 gagcccggcg ccgacgacgc gaccgaggcc gtctccgagc ccgaggccgt ccccgcgccc
      121 gatggcgagg acgcgcccga gccggcggcc gatgagcccc acgccgaggc tgccccggaa
      181 cccgagcctg agcccgagcc cgagcccgag ccggagcccg agcccgagcc cgaagctgat
      241 gaagcggcgg acgacgaggc cgacgaagcc gacgatgacg aggtcgacga ggccggcgac
      301 gacgaacccg gagctgttcc cgccgccgcg gcggctgccg cggccacgac aacggctcgt
      361 cgggccaagg atcgaccgcc gacgaagtcg gccccggccg cgccgaccgt ctcggagcag
      421 gcggtccaca tcaacgatcg ggcgtcggcg atcttcgtga tcgtcgtcgt cggcgtcttc
      481 gccatcatct tcctgaacgc gctgctcctc gggcacaagg gcctgctctc ggggctgctg
      541 ccgcaaccca cgcccatcgc cagcgaggca ccgtcggcga gcgcgggacc atcggcgtcg
      601 cccagcgggt cggcggccgc gtcgccgagc gggtcgcccc cggcgtcgcc atcggccgct
      661 gcgtcgccat cggccgctgc atcgccatcg gccgctgcat cgccatcggc cgaaccatca
      721 cccagcgcgg cgtcgcccgc ggcgccgtcg ccgagcccag cggccagctg agcccgcgac
      781 gatggacgca gcccgggcgc gcgcgagcga ggcccgggcc cggatggtcg ccgaccagct
      841 ccggaagcgt gacatccgcg acgagcgcgt cctctcggcg atgaccgcgg tcgagcgtga
      901 cgccttcgtg cccgagcgcg accgggacgc cgcgtacacc gactcgccac tgccgatcgg
      961 cggcggccag acgatcagcc agccgtatat cgtggcccgc atgacggagc tcctggcggt
     1021 cgagtcgggc gaccgggtgc tcgacgtcgg gaccggctcc ggctaccagg cggcggtcct
     1081 cgcggagctc ggctgcctgg tgacgtcgat cgagcgcgac cccgacctcg cgttggccgc
     1141 gatggaccac ctcgtcgccg ccgggtacgg cgaccgggtc atcgtgcgcg tcggcgacgg
     1201 gagcctcggt gcccccgacg aggcgccctg ggacggcatc gtcgtcgcgg cgggtgcccc
     1261 ggcgatcccg gagccgcttc gggagcagct cggcgagggt cggcggctgg tgatcccggt
     1321 cggcggcctg cgggagcaga gcctgatggt ggtcgagcgc cacggcgatg aatggacgga
     1381 acagtcggac gggccgtgcg tcttcgtgcc gctggtgggc gccgaaggct ggaccgcctc
     1441 ggcgctgtga gcctgctgct gggcggcaaa tccgtcgcgc ccctgttccg ccgcctcgga
     1501 ccggtgttcg cggtcgccgg cgccgtcgtc gccgcggtga cgtggccgcg ggccttcgac
     1561 ctggaggcgt acctcgacgc cgcgaagctc gtcgcggctg gtcaggaccc gtatgcggcg
     1621 acccgggcgc tgggtgtggc cgagtggggc cagggccagg tgtacgtctc gcccccgttc
     1681 atcgc
//
LOCUS       DBNHXO010000338         2849 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4859380, whole genome shotgun sequence.
ACCESSION   DBNHXO010000338 DBNHXO010000000
VERSION     DBNHXO010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4859380"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="AC162A_07060"
     CDS             complement(<1..761)
                     /locus_tag="AC162A_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HIR5010638.1"
                     /translation="MGTARLTTVDLPDFGMPEVRPEVPGDRYGKRLAALRERADAAGF
                     DRLVVYADREHSANLAFLSGFDPRFEEAMLIVGPASDPLILVGNECFGMAGAAPLPMR
                     RERFQDFSLPSQPRDRSRPLRDILAGEGIGPGSRVGVLGWKTYADPGLIEIPAFIVDE
                     LRGLVGATGSVRNANGLLIDPADGLRIINDVDQLAVFEHASCQTSDGLRRLLAGLELG
                     MTEAEAVRLLRWNGMPLSCHLMLTAGPRAALGLLSP"
     gene            complement(777..2414)
                     /locus_tag="AC162A_07065"
     CDS             complement(777..2414)
                     /locus_tag="AC162A_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR5010639.1"
                     /translation="MLAAATVVVVALVVVGGVSAFVVASGVPRTPVAPRFVEETATAG
                     IDHTFDGGLKFFTGGGVAVLDCDGDGRPDVYLAGGEHPAGLYRNETAVSGPLRFTQVP
                     DSGTELSAVTGAYPLDVDADGFDDLAVLRVGQSMLLRGLGDCRFEPANEAWGVSPPPA
                     WTTAFSATWEGAAARPTMAFGNYVALDAAGEVASGCADSELFRPDATGTGYGPAIPLA
                     PGYCTLSILFSDWDGSGRRDLRVTNDRHYYATGNDQLWRVAPGEPPREYTAADGWVPM
                     QIWGMGIASHDLTGDGLPEVYLTSQADNKLQTLAEGPSRPVYGDIALRRGVTASQPFT
                     GGDVLPSTAWHPEFQDVNNDGLMDLFVSKGNVDEMPDYARRDPSNLFIGQPDGTFLEG
                     AEAAGIVSFDKGRGAALTDFNADGLLDLIEANLGAPVRLWRNVGTGTIDAPVPMGNWL
                     AVRLGQPGGNRDAIGAIIESRVGEAVTRRETSVGGGHIGGQLGWTHVGLGPATEAQVR
                     VTWPDGEVGPWMTIAANQFVDIERGAPVPAPAMPPGS"
     gene            complement(2544..>2849)
                     /locus_tag="AC162A_07070"
     CDS             complement(2544..>2849)
                     /locus_tag="AC162A_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR5010640.1"
                     /translation="TCSTRTETDQRVHVYGTVGRISVEIPFNIPPDRPTRVFVTAGGD
                     PPVAPATETLTFATADPYACEADRFAEAVLDGVPLPVEPEDAVANMRVLDALFASGA"
BASE COUNT          404 a         1046 c          997 g          402 t
ORIGIN      
        1 gggctcagca gcccgagcgc cgcacgcggg ccggcggtga gcatcaggtg gcacgacagc
       61 ggcatcccgt tccatctgag caggcggacc gcctcggcct cggtcatccc gagctcgagg
      121 ccggcgagca gccgccgcag gccgtccgag gtctggcacg aggcgtgctc gaagaccgcc
      181 agctggtcga cgtcgttgat gatccggagg ccgtccgcgg gatcgatgag caggccgttc
      241 gcattgcgga cggagccggt cgcccccacg aggccacgga gctcgtccac gatgaacgcc
      301 gggatctcga tcagcccggg gtcggcgtac gtcttccagc ccaggacccc gacccgcgac
      361 cccggcccga ttccctcccc tgccaggatg tcgcgcagcg ggcgcgaccg gtcgcgcggc
      421 tggctcggca ggctgaagtc ctggaatcgc tcgcggcgca tcgggagcgg cgcggcgccg
      481 gccatcccga aacactcgtt gcccacgagg atcagcgggt cgctggccgg cccgacgatc
      541 agcatcgcct cctcgaatcg cgggtcgaac ccggagagga acgcgagatt cgcgctgtgc
      601 tcgcggtccg cgtagaccac gagccggtcg aagccggcgg catcggcgcg ctcgcgcagc
      661 gccgccaggc gcttgccgta ccgatcaccc ggaacctccg gacggacttc gggcatcccg
      721 aagtcgggca ggtcgaccgt cgtgagcctc gccgttccca aggtcgatcc tcctcctcag
      781 ctgcccggtg gcatcgcggg cgccggcacg ggtgcgccac gctcgatgtc cacgaactgg
      841 ttggccgcga tcgtcatcca cggtccaacc tcgccgtccg gccaggtcac ccgcacctgc
      901 gcctcggtcg ccggtccgag cccgacgtgg gtccagccga gctggccacc gatgtggccg
      961 ccgccgaccg aggtctcccg ccgggtgacg gcctccccca ccctgctctc gatgatggcg
     1021 ccgatcgcat cccggttgcc gcctggttgg cccaggcgca cggccagcca gttgcccatc
     1081 gggaccggcg catcgatcgt accggtcccc acgttgcgcc acaggcgcac cggcgcgcca
     1141 aggttcgcct caatgaggtc gaggagaccg tccgcgttga agtccgtcag cgccgcgcca
     1201 cggcccttgt cgaacgacac gatgcccgcc gcttcggcgc cttcgaggaa ggtgccgtcc
     1261 ggttggccga tgaacaggtt gctcgggtcc cgccgggcgt agtccggcat ctcgtcgacg
     1321 ttgcccttgg acacgaacag gtccatcagc ccgtcgttgt tgacgtcctg gaactcggga
     1381 tgccaggccg tcgaaggcag gacgtcaccc ccggtgaacg gctgggacgc ggtgacgccg
     1441 cggcgcaacg cgatgtcgcc atacacgggc cgcgacggtc cttccgcgag cgtctggagc
     1501 ttgttgtccg cctggctcgt gaggtagacc tcgggcagcc cgtcgcccgt gaggtcatgg
     1561 ctcgcgatcc ccatgcccca gatctgcatc gggacccaac cgtccgccgc cgtgtactcc
     1621 cgcggcggct cgcccggggc cacgcgccac agctggtcgt tgcccgtcgc gtagtagtgg
     1681 cgatcgttgg tgactcgcag gtctcgccgg cccgatccgt cccagtcgct gaacaggatc
     1741 gagagcgtgc agtagccggg cgcgagcggg atcgcgggtc cgtatcccgt gccggtcgcg
     1801 tccggccgga acagctcgct atccgcgcag ccggacgcga cctcgccggc cgcgtcgagg
     1861 gccacgtagt tcccgaacgc catcgtgggc cgtgcggccg cgccctccca ggtcgccgag
     1921 aacgccgtcg tccacgcggg cggcggcgag accccccacg cctcgttcgc cggttcgaac
     1981 cggcagtccc caagaccccg gagcagcatc gactggccca cccggaggac cgcgaggtcg
     2041 tcgaacccgt cggcgtcgac gtcgagcggg tatgcgccgg tcaccgcgga gagctccgta
     2101 cccgaatccg ggacctgggt gaatcggagt gggccgctga ccgcggtctc attgcggtac
     2161 aggccggccg ggtgctcgcc gccggccagg tagacgtcag gccgcccatc gccgtcgcag
     2221 tcgagcacgg cgaccccgcc gccagtgaag aacttcagcc cgccgtcgaa ggtatggtcg
     2281 atcccggcag ttgccgtctc ctccacgaaa cgcggcgcga ccggcgttct ggggacaccc
     2341 gacgcgacga cgaacgccga gacaccgccg accacgacca gcgccacgac gaccacggtt
     2401 gcggcggcca gcaacgttcg gcggctggcg cgctcacggc gctcgtcgcc gcccgtgacg
     2461 tcctgtggct ctgacaggcc gcgcgatggt acgtcccctt gccgggccgg gctcggggtg
     2521 agccggaacg gggtccgccg tgttcaggcg cccgaggcga acagcgcgtc gaggacccgc
     2581 atattggcga ccgcgtcctc gggttccacc ggcagcggga cgccgtcgag caccgcctcc
     2641 gcgaaccggt cggcctcgca ggcgtaggga tcggcggtgg cgaaggttag cgtctcggtc
     2701 gccggtgcca ccggagggtc gccgcccgcc gtgacgaaga cccgggtggg gcggtcgggc
     2761 gggatgttga acgggatctc gaccgagatc cggcccacgg tgccatagac gtggacgcgt
     2821 tggtccgtct cggtccgcgt ggagcaggt
//
LOCUS       DBNHXO010000339         2214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5057988, whole genome shotgun sequence.
ACCESSION   DBNHXO010000339 DBNHXO010000000
VERSION     DBNHXO010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5057988"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="AC162A_07075"
     CDS             <1..705
                     /locus_tag="AC162A_07075"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5010641.1"
                     /translation="LLPEGTEAYVKSVIEEQLPGIAAHGRATSCDVFCEEGVFTADQS
                     RRILQAAAGYGMAVRLHADELAPSGGAELAAELGALSADHLHTPSDAGVEALAEAAGG
                     DNPTVATLLPATTWFLMADEAAPARRFIEAGVPVALGTDFNPGTSPTPNLPLVTTVAC
                     LEMKLTPEEALVGVTINAAHAVGVAEQVGSLEVGKQADVVVWRAASVAELPYWVGANL
                     CEVVVKAGHIVYRRGG"
     gene            complement(851..1522)
                     /locus_tag="AC162A_07080"
     CDS             complement(851..1522)
                     /locus_tag="AC162A_07080"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5010642.1"
                     /translation="MELTWYGRTCIRQKGRDAVVVNDPYPAVVGPTGRGITGDVVTFS
                     HPDDAPKPKKAVRLSRDGSTAIPSSLEPAFVLDGPGEYEVHDALVTGVRTYRDDAKGT
                     ERGRQVAFVTELDGIHTIHLGDIGHQLTEEKLADIGRVEVACVPIGGALSATRAAALI
                     AQLDPKIVVPMTVCEDEADCDEALKKFLHEMGATPTVQSKLSVTSSSLPLETTTVLLE
                     SRGKV"
     gene            1794..>2214
                     /locus_tag="AC162A_07085"
     CDS             1794..>2214
                     /locus_tag="AC162A_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010643.1"
                     /translation="MTSTPPRLAPFRASLLTLLVVAAAACGQSTATPTPTARSSSPAA
                     TASPTAAPTPPPTPAPTMAAVDVCADAAAGAGPSSAPPEQPSPDADDPNAARYEEIEG
                     QVSALRGLTLTTPVERDVFNREELGTFIQGSFGRDNPE"
BASE COUNT          306 a          767 c          814 g          327 t
ORIGIN      
        1 ctgctgcccg agggcacgga ggcgtacgtc aagagcgtga tcgaggagca gctgccgggc
       61 atcgcggcac acgggcgggc gacgtcgtgc gacgtgttct gcgaggaggg cgtgttcacg
      121 gccgaccaga gccggcggat cctccaagcg gcggccgggt acgggatggc cgtccgcctt
      181 cacgccgatg agctggcgcc gtcgggcggc gcggagctcg ccgcggagct gggtgccctg
      241 tcggccgacc acctccacac gccgagcgat gccggggtcg aggcgctggc ggaggctgcc
      301 gggggcgaca acccgacggt cgccacgctg ctcccggcga cgacctggtt cctgatggcc
      361 gacgaggcgg cacccgcgcg gcggttcatc gaggcgggcg tcccggtggc cctcggcacc
      421 gacttcaatc ccgggacctc gcccacgccg aacctcccgc tggtgacgac ggtggcctgc
      481 ctcgagatga agctgacgcc cgaggaggcg ctggtgggcg tgacgatcaa cgccgcccac
      541 gccgtgggcg ttgcggagca ggtggggtca ctcgaggtgg gcaagcaggc cgacgtcgtc
      601 gtgtggcgag ccgcgagcgt cgcggagctg ccgtactggg tcggcgccaa cctctgcgaa
      661 gtcgtcgtaa aggcggggca catcgtgtat cgccggggcg gctgatccgc cgccatcgcg
      721 gcgttgtcgg ctgcggtcct cgctcgcgca ccccgaagtg cgctcgctcc ggtcctctcc
      781 tccgccttgc gctgacggcg gctgagccgc ccgtgtcagc gggcggtcgg ttgggccctt
      841 gggtagcggg tcagaccttg ccgcgggatt ccaggaggac cgtggtggtc tcgaggggga
      901 ggctcgagga tgtcaccgag agcttcgact ggaccgtggg cgtcgcgccc atctcgtgga
      961 ggaacttctt gagcgcctcg tcgcagtcgg cctcgtcttc gcagaccgtc atcggcacga
     1021 cgatcttggg atcgagctgg gcgatcaggg ccgcggcacg ggtggcgctc agcgcaccgc
     1081 cgatcggcac gcatgcaacc tcgacccggc cgatgtcggc gagcttctcc tcggtcagct
     1141 ggtggccgat gtcgccgagg tggatcgtgt ggatcccgtc gagctcggtg acgaacgcga
     1201 cctggcggcc ccgctccgtg cccttggcgt cgtcgcggta ggtacggacg cccgtgacca
     1261 gggcgtcgtg gacctcgtac tcgcccgggc cgtcgagcac gaacgcgggc tcgaggctgg
     1321 acgggatcgc ggtcgagccg tcgcgcgaca gccggacggc cttcttgggc ttgggcgcat
     1381 cgtcggggtg gctgaacgtg accacgtcgc cggtgatgcc gcgtccggtc gggccgacga
     1441 cggcggggta ggggtcgttg acgacgacgg cgtccctgcc cttctggcga atgcaggtgc
     1501 gtccgtacca ggtgagttcc atgggcccga ttcttgcaca ggccgctcgc ccggggctca
     1561 ccccggcgcc gctcccagcg acccgctgga atcgaaagga ccggaagcgc ctgcccaatc
     1621 ggcgcctccg gtccgaggga gggaggatgg aacctgttga gggtttccga ggggaaccat
     1681 aggccgcgcc cgattcagcg ccgagatctc gaacggttaa gaaacgcaca tctcgggggg
     1741 ttcgaggggt gccgaaccag tccaaagcga ttcccgtggc agaatgccgc gccatgactt
     1801 cgacaccccc tcggctcgcg ccgttccgcg cgtcgctcct gaccctgctc gtcgtcgctg
     1861 ccgccgcctg cggccagtcc accgcgacgc caacaccgac ggcccgatca agctcgcccg
     1921 cggcaaccgc gtcgcctacg gccgcaccga cgccgccgcc gacgcccgcg ccgaccatgg
     1981 ccgcggtcga cgtctgcgcc gacgcagcgg cgggtgccgg gccgtcgtcc gcgccgcccg
     2041 aacagcccag tcctgacgcc gacgatccga acgccgcgcg ctacgaagag atcgaaggcc
     2101 aggtgtcggc gctgcgcggc ctgacgctca cgacgcccgt cgagcgcgat gtgttcaacc
     2161 gcgaggagct gggcacgttc atccagggtt cgttcggccg ggacaacccc gagt
//
LOCUS       DBNHXO010000340         3606 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3667282, whole genome shotgun sequence.
ACCESSION   DBNHXO010000340 DBNHXO010000000
VERSION     DBNHXO010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3667282"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1438)
                     /locus_tag="AC162A_07090"
     CDS             complement(155..1438)
                     /locus_tag="AC162A_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HIR5010644.1"
                     /translation="MSSRRGTVEPPRVDTAALLDDLVSLVRIPSVTGAEDAIAARLAD
                     VLEALGMRVEVFHPDPAAIRDDPAWPGEEVARSTLPVVLGRAGRPGGRRGVLSGHLDV
                     VPPGDPATWTGDPWGANVRDGRLYGRGACDMKGGVASILGAVRALRDAGTLDALDGEL
                     IVAFVPSEEDGGQGTLAAIRAGATGDMAIITEPSGLDIVVAHAGAITFRLTVPGRAAH
                     ASRRTEGVSALDNLGTLVRALEADEERRNEAETEPLMTALGLPYPTIIGMVAGGEWAS
                     TVIDRITADGRYGVRLGQTPEAAEAELRACIAAACAGDDFLAQHPATVEISGARFASS
                     SVPVAHRLVTGLAAATSEVTGRRPGLVGVPYGCDMRLFIGVGETPCVVFGPGDVRLAH
                     AADESVPLAEVEACAGVLAAWVVRELGTGWAPTRR"
     gene            complement(1435..2304)
                     /locus_tag="AC162A_07095"
     CDS             complement(1435..2304)
                     /locus_tag="AC162A_07095"
                     /EC_number="3.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF039614.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016403 - dimethylargininase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylarginine dimethylaminohydrolase family
                     protein"
                     /protein_id="HIR5010645.1"
                     /translation="MTVVSSRTFGAQSMTAPLRDVLVKAPGSAFGRAFEDPAHGFLRP
                     VDLDLARRQHDGLVGALDRLGVRVRVLDAETDDPDLVYVFDPLLVADGGAIPLRPGKP
                     NRAAEPALLERWTAAAGIPTLGRIEAPGTIEGGDTFWLRPDLLCIGRTLRTNEAGARQ
                     LAAIAGGDVRVFDVPYWKGPAELVHLLSVISPVADDVAVVFLPLLPAGLYELLADLGV
                     RLVEVPEDEYATLACNVLAVRPGVVIVADGNPATRRGLEAAGCEVHAIPLDEVGENGS
                     GGVTCLTRPILRG"
     gene            2414..>3606
                     /locus_tag="AC162A_07100"
     CDS             2414..>3606
                     /locus_tag="AC162A_07100"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR5010646.1"
                     /translation="MTNSTAFAPRSFLRSAAAGAVLASTLLAGLLPARAAALSATPLT
                     DGAGALSAPSGSTIDLTPVASGLANPVLFTSAHDGTGRMFIVEQTGAIRVFDNGSVLP
                     GNLLNLAGSISHGDEQGLLGLAFHPNFENNRKFYVNYTNRGGNTVVREYKTSISNPNR
                     VQSGSGRTILKVHQPYSNHNGGNLAFGPGGYLYIGLGDGGDAGDPGRRAQDKNSLLGK
                     MLRIDVDSRSGNKAYGIPKSNPYVGEKGKNEIFQRGLRNPWRYSFDRSNGNLWIGDVG
                     QGGYEEIDRTTSGNGTNWGWRVMEGYKCYSPSSGCNKDGKKKPVAVYSHANGRCAVTG
                     GYVYRGSNIPALRGWYVFGDYCSGEIWAVSSTASDHKSKILLRDSNASISSFGENAGG
                     ELFVVG"
BASE COUNT          537 a         1335 c         1279 g          455 t
ORIGIN      
        1 gccgccagcc tcggctcggg gctgatcatg gccgcggtcg gctacaccgc gctggggatc
       61 ctcggggccg ggttggtcgt gatcccgatc ctcgtcctgc gggcccagcg gcgggcggcg
      121 atccgcagcg cggacgtcgg gagcccgatc gacatcatcg cctcgtaggc gcccagccgg
      181 tccccagctc ccggacgacc cacgccgcga gcacgccggc gcaggcctcg acctcggcca
      241 gcgggacgga ctcgtcggcg gcgtgcgcca gccggacgtc cccgggaccg aacacgacgc
      301 acggcgtctc gcccacgccg atgaacagcc gcatgtcgca gccgtaggga acgccgacca
      361 ggcccggccg ccggcccgtc acctcgctcg tggctgccgc caggcccgtg accagccgat
      421 gagcgaccgg cacggacgac gaggcgaacc gggcgccgct gatctcgact gtggccgggt
      481 gctgcgcgag gaagtcatcg cccgcgcagg cggccgcgat gcacgcgcga agctcggcct
      541 ccgccgcctc gggcgtctga cccaggcgga cgccgtaccg gccgtcggcg gtgatcctgt
      601 cgatgaccgt ggaggcccac tccccgccgg cgaccatccc gatgatcgtc gggtacggga
      661 ggccgagggc cgtcatgagg ggctcggtct cggcctcgtt gcggcgctcc tcgtccgcct
      721 cgagggcccg aacgagcgtc cccaggttgt cgagcgccga cacgccctcg gtccggcggc
      781 tggcgtgcgc cgcgcggccc gggacggtga gccggaacgt gatggcgccc gcgtgggcga
      841 cgacgatgtc gagccccgac ggctcggtga tgatcgccat gtcgcccgtc gcgcccgccc
      901 ggatcgccgc cagcgtcccc tggccgccgt cctcctcgga cggcacgaac gcgacgatga
      961 gctcgccatc cagcgcgtcc agcgtgccgg cgtcccgcag cgcccgcacc gcgcccagga
     1021 tcgacgcgac accacccttc atgtcgcacg cgccgcgtcc gtacagccgg ccgtcgcgga
     1081 cattcgcccc ccatggatcg ccggtccacg tcgccggatc gccgggcggg acgacgtcga
     1141 ggtgtcccga cagcaccccg cgccgcccgc cgggtcgtcc ggcacgcccg aggacgacgg
     1201 gcagcgtcga cctcgccacc tcctcgccgg gccaggccgg gtcgtcgcgg atcgcggccg
     1261 gatccgggtg gaacacctcg acccgcatgc ccagcgcctc gagcacgtcg gccaggcgcg
     1321 cggcgatcgc gtcctcggcg ccggtgacgc tggggatcct gacgagcgac acgaggtcgt
     1381 cgagcagcgc ggcggtgtcg accctcggcg gctcgacggt gccccgccga gagctcaccc
     1441 gcgcaggatc ggccgggtca ggcaggtcac gccgcccgac ccgttctccc ccacctcgtc
     1501 gagcgggatc gcgtggacct cgcagccagc ggcctccagg ccccgccggg tcgctgggtt
     1561 gccatcggcg acgattacca cgcccggacg cacggcgagc acgttgcagg cgagtgtcgc
     1621 gtactcgtcc tccggcacct cgacgagccg cacgccgagg tccgcgagca gctcgtacag
     1681 ccccgcgggg agcagcggca ggaacacgac cgcgacgtcg tcggcgacgg gggagatcac
     1741 cgacagcagg tggacgagct cggccgggcc cttccagtac gggacgtcga acacgcggac
     1801 gtcgccaccc gcgatggccg cgagctggcg ggcgccggcc tcgttcgtgc gcagcgtccg
     1861 gccgatgcac agcaggtcgg ggcggagcca gaacgtgtcg ccgccttcga tcgtgccagg
     1921 cgcctcgatt cggcccaggg tcgggatccc ggccgcggcc gtccaccgct cgaggagcgc
     1981 gggctcggcc gcccggttgg gcttgccggg ccgcagcggg atcgcgccgc cgtccgccac
     2041 caacagcggg tcgaagacat agacgaggtc cgggtcgtcg gtctcggcgt cgaggacgcg
     2101 aacccggacg ccgaggcgat cgagggcgcc caccaggccg tcgtgctggc gccgggcgag
     2161 gtcgaggtcg acggggcgga ggaagccgtg cgccgggtcc tcgaacgcgc gaccgaaggc
     2221 gcttccgggg gccttgacga ggacgtcgcg caacggcgcg gtcatgctct gggcgccgaa
     2281 ggtacggctg gaaacgacgg tcacggaacg gagaatacga ccctggttcg acgcacgcgc
     2341 cgaatcgggg caggattcgg gctgcgccgg acgcgatccg ggccccgtcc acaacacgcc
     2401 gcagggtgac ttcgtgacca actcgaccgc cttcgcgccc cgctcgttcc tccggtccgc
     2461 cgcggctggc gccgtcctcg cgtcgacgtt gctcgccggc ctgctgcccg cccgggcggc
     2521 cgcgctcagc gcgacgccgc tcacggacgg ggcgggtgcg ctcagcgcgc cgtcgggctc
     2581 gacgatcgac ctcacgccgg tggccagcgg cctcgcgaac ccggtgctgt tcaccagcgc
     2641 acacgacggt acgggacgca tgttcatcgt cgagcagacg ggtgcgatcc gcgtcttcga
     2701 caacggctcc gtgctccccg gcaatctgct gaacctcgcc ggctcgatca gccacggcga
     2761 tgagcaggga cttctgggtc tcgcctttca tccgaacttc gagaacaacc gcaagttcta
     2821 cgtgaactac accaaccgcg gcggcaacac cgtggtccgc gaatacaaga cgtcgatctc
     2881 gaaccccaac cgcgtgcagt cgggttcggg ccggacgatc ctcaaggtcc accagccgta
     2941 ctcgaaccac aacggcggca acctggcgtt cgggccgggc ggctacctgt acatcggcct
     3001 cggcgacggc ggcgacgcgg gcgaccccgg ccgccgggcc caggacaaga actccctgct
     3061 gggcaagatg ctccggatcg acgtcgacag ccggagcggc aacaaggcct acgggatccc
     3121 caagtcgaac ccgtacgtcg gcgagaaggg gaagaacgag atcttccagc gcgggctgcg
     3181 caacccctgg cgctactcgt tcgaccgcag caacggcaac ctgtggattg gcgacgtcgg
     3241 ccagggcggg tacgaggaga tcgaccggac cacctccgga aacggcacca actggggctg
     3301 gcgggtcatg gagggctaca agtgctactc gccctccagc ggctgcaaca aggacggcaa
     3361 gaagaagccg gtcgctgtct actcgcacgc caacgggcgg tgcgcggtca ccggcggtta
     3421 cgtctaccgc gggtcgaaca tcccggcgct gcggggctgg tacgtgttcg gcgactactg
     3481 cagcggcgag atctgggcgg tctcgtcgac cgcgtccgac cacaagtcga agatcctgct
     3541 gcgcgactcg aacgcgtcga tcagctcgtt cggcgagaac gccggcggtg agctgttcgt
     3601 cgtcgg
//
LOCUS       DBNHXO010000341         4622 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1289071, whole genome shotgun sequence.
ACCESSION   DBNHXO010000341 DBNHXO010000000
VERSION     DBNHXO010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1289071"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..2830)
                     /gene="acnA"
                     /locus_tag="AC162A_07105"
     CDS             complement(158..2830)
                     /gene="acnA"
                     /locus_tag="AC162A_07105"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR5010647.1"
                     /translation="MPRTNAFGARASLGAGLPDLYRLASLSTSGLALGVDLGRAPVTL
                     KILLENALRHAGGGIVRDEDVSALAAWRPGGAGEAEIPFMPSRVVLQDFTGVPAVVDL
                     AAMRDAMAGLGGDPARVNPLVPADLVIDHSVQIDAWGGSGAFAINVEREYERNGERYQ
                     LLRWAQTAFRDLRVVPPGTGIIHQVNLEFLATVVADREDADGRVAFPDTLVGTDSHTT
                     MINGLGVLGYGVGGIEAEAVLLGQPLYQPMPRIVGVRLTGELPTASTATDLVLVITEM
                     LRKFGVVGSFVEFAGDGLAGLALADRATISNMSPEFGATATLFPIDDETLTYLRLTGR
                     PAARVDLVERYAKAQGLWREPGNGPEFDVALELDLATVEPSLAGPRRPQDRVALAGLG
                     ANFRAAYPQTEGVERRPIAGAGEAPPFGHGSVAIAAITSCTNTSNPTVMVGAGLLARN
                     AVERGLRVSPTVKTSLAPGSKAVTGYLERAGLMAPLGALGFALAGYGCTTCIGNSGPL
                     AEPVAAAIESDELVVAAVLSGNRNFEGRIHPLVRAAYLASPPLVVAFALAGRVDVDLT
                     TEPLGTGSDGRPVMLADIWPTPAEIRSVIADSIDPELFRQTYASVFAGDERWTALPIP
                     SGDRYAWDDASTYIARPPFFEGLTTEPAPLEDIDGARVLALLGDSVTTDHISPAGSIA
                     AWSPAGEWLQAHGVGPLEFNSYGARRGHHEVMMRGTFANIRLRNLLVDGREGPYTRHL
                     PDGEEAFIYDAAMRYRAEGVPLLVIAGREYGSGSSRDWAAKGTTLLGVRAVLAESYER
                     IHRSNLVGMGVLPLQFLPGDSAASLGLTGLEAYSINGLATLRPRQEVTVVARADDGTE
                     HQFRAIARLDGPIEVDYLRQGGILPAVLRRLALG"
     gene            2970..4004
                     /locus_tag="AC162A_07110"
     CDS             2970..4004
                     /locus_tag="AC162A_07110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HIR5010648.1"
                     /translation="MLRRAAERRDAEHEETLHELSDEGVDLDPATPGHHRHGDIEHAS
                     DHAHPHAHSGPGERPVIGIVGAGPVGTALGVALDRAGWPVGAVASRDPGRRAQFREKV
                     AGARGFAEANALVDDVELVILAVPDDAIVAVAESIRLYAGQALIHTSGVLEAEALRPA
                     MAAGTQAGAFHPLVAFADLERALAALPGATIAIEGDDELAAHLGDMAEAIGGLPVRLP
                     PGTKAAYHAAAVLAAGGVVALLDTVREIAGLLGLDEAGAMDVYLPLLEQAVANARALG
                     IAASLTGPATRGDIGTIGAHLAALEAGAPDALPVYRALLTRGAGIALDRGALSPESAE
                     RLSAALAGNR"
     gene            4273..>4622
                     /locus_tag="AC162A_07115"
     CDS             4273..>4622
                     /locus_tag="AC162A_07115"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5010649.1"
                     /translation="MDELHPGRGIAPLLRGARVARLRQATATSAGGIVIRSTGGTPEL
                     VVGMRRRERDGVTWTLPKGTPIPNETTEQTAVREVREETGLEVRIISPFDHIDYTFVM
                     GGTRISKTVHYFLMV"
BASE COUNT          634 a         1658 c         1749 g          581 t
ORIGIN      
        1 ggattcaact tcgcgctgac gccgcgtcac accatccgcg gtcgcaccgt cgcaggaacc
       61 gaagctccct cgagggtgcg gcctcctccc cgtgcgtcga gggtcctggt cacgtgaagc
      121 ctccggcatc gccgggggct tcacgccgtt cgggcgatca gccgagggcc aggcgccgca
      181 gcaccgccgg caggatcccg ccctgacgca ggtagtcgac ctcgatcggg ccgtcgagcc
      241 gggcgatcgc gcggaactgg tgctccgtgc cgtcgtcggc gcgtgcgacc acggtcacct
      301 cctggcgtgg ccgcagggtc gccagcccgt tgatcgaata cgcctcgagg cccgtgagcc
      361 cgagcgacgc ggcgctgtcg ccgggcagga actggagcgg caggacgccc atcccgacga
      421 ggttcgagcg gtggatgcgc tcgtagctct cggccagcac cgcccgtacg ccgagcagcg
      481 tcgtgccctt ggcggcccag tcgcgcgacg accccgaacc gtactcgcgg ccggcgatga
      541 ccagcagcgg cacgccctcg gctcggtagc gcatggcggc gtcgtagatg aacgcctcct
      601 cgccgtcggg aaggtggcgc gtgtaggggc cctcgcgacc gtccacgagc aggttgcgaa
      661 gccggatgtt ggcaaaggtg ccgcgcatca tcacctcgtg atggccgcgg cgggcgccgt
      721 aggagttgaa ctcgagcggc ccgacgccgt gcgcctggag ccactcgccg gcgggcgacc
      781 aggcggcgat cgaaccggcc gggctgatgt ggtcggtcgt gaccgagtca ccgagcagcg
      841 ccagcacccg cgcgccgtcg atgtcctcga gcggtgcggg ttcggtggtc aggccctcga
      901 agaacggcgg ccgggcgatg taggtggagg cgtcgtccca ggcgtagcgg tcgcccgacg
      961 gaatcggcag ggccgtccag cgctcgtcgc ccgcgaacac cgaggcgtag gtctgccgga
     1021 acagctcggg gtcgatggag tccgcgatga ccgaccggat ctccgccggc gtcggccaga
     1081 tgtcagcgag catgaccggc cggccgtcgc ttcccgtgcc cagcggctcg gtggtgagat
     1141 cgacgtcgac ccggcccgcg agcgcgaacg cgacgacgag cgggggcgag gcgagatacg
     1201 cggcccggac gagcgggtgg atccgcccct cgaagttgcg gttgcccgac agcacggccg
     1261 cgacgacgag ctcgtcggac tcgatggcgg cagccactgg ctccgccagc ggcccggagt
     1321 tgccgatgca ggtggtgcag ccgtagcccg ccagggcgaa gcccagcgcg ccgaggggag
     1381 ccatgaggcc ggcccgctcc aggtagccgg tgacggcctt ggagcccggg gcgagcgacg
     1441 tcttgacggt cggggagact cgtagcccac gctcgacggc gttgcgggcg aggagcccgg
     1501 cgccgaccat cacggtcggg ttggacgtgt tggtgcagga cgtgatggcg gcgatcgcga
     1561 ccgagccgtg tccgaacggc ggcgcctcgc cggcgccggc gatcggccgc cgctcgacgc
     1621 cctcggtctg cgggtaggcg gcgcggaagt tggcgccgag gccggcaagg gcgacccggt
     1681 cctgcggccg ccgcgggccg gccagcgagg gctcgacggt cgcgaggtcg agctccagcg
     1741 cgacgtcgaa ctccgggccg ttcccgggct cgcgccacag gccctgggcc ttcgcgtaac
     1801 gctcgacgag gtcgacgcgg gcggccggcc gcccggtgag ccgcaggtac gtcagggtct
     1861 cgtcgtcgat cgggaacagc gtcgcggtgg cgccgaactc ggggctcatg ttcgagatcg
     1921 tcgcccgatc ggcgagcgcg aggccggcca ggccgtcgcc ggcgaactcg acgaaggagc
     1981 ccacgacacc gaacttgcgc agcatctcgg tgatgacgag gacgaggtcc gtcgccgtcg
     2041 acgccgtcgg cagctcgccg gtgagccgga cgccgacgat gcgcggcatc ggctggtaca
     2101 gcggctggcc cagcagcacg gcctccgcct cgatgccgcc gacgccgtag ccgagcacgc
     2161 ccaggccatt gatcatcgtc gtgtgggagt cggtcccgac gagggtgtcg gggaaggcga
     2221 cccggccgtc cgcgtcctcg cggtccgcga cgacggtcgc caggaactcg aggttgacct
     2281 ggtggatgat tccggtgccc ggcggcacga cccgcaggtc ccggaacgcg gtctgggccc
     2341 agcgcaacag ctggtagcgc tcgccgttgc gctcgtactc gcgctccacg ttgatcgcga
     2401 acgcgcccga gccgccccag gcatcgatct gcacggagtg gtcgatgacc aggtccgccg
     2461 gcaccagcgg gttgacccgc gccgggtcgc cgccgaggcc ggccatcgca tcgcgcatcg
     2521 ccgcgaggtc gaccaccgcc ggcacgcccg tgaagtcctg gagcaccacg cggctgggca
     2581 tgaacgggat ctcggcctcg cccgcgccac ccgggcgcca ggcggcgagc gcgctcacgt
     2641 cctcgtcgcg gacgatgccg ccgccggcgt ggcggagggc gttctcgagc aggatcttga
     2701 gcgtcaccgg cgcccggccg aggtcgacgc cgagggcgag ccccgatgtc gagagcgacg
     2761 ccaggcggta caggtcgggg aggccggcgc cgagggaagc gcgggccccg aatgcgttgg
     2821 tcctgggcat ggtgatcctc ctcgggggat cgggcgatga gggtgcgccc tcggaccgct
     2881 cgctacactc gttgcgtgga agcgtaccgc gcgaacgccc cgggagcagg ggagccgccc
     2941 aggtgagggc ctgggacgag acggaggacg tgctccgtcg ggccgccgag cgccgcgacg
     3001 ccgagcacga ggagacgctg cacgagctca gcgacgaggg ggtcgacctc gaccctgcca
     3061 cccccgggca ccatcggcac ggcgacatcg agcacgcgag cgaccacgcg cacccccacg
     3121 cgcactcggg cccgggcgaa cgaccggtga tcgggatcgt gggcgccggg ccggttggca
     3181 cggcgctcgg ggtggcgctc gaccgggcgg gctggccggt gggcgccgtc gcgtcgaggg
     3241 acccgggccg tcgcgcgcaa ttccgggaga aggtggccgg cgcccgcgga ttcgcggagg
     3301 ccaacgcgct ggtcgacgac gttgagctcg tgatcctcgc ggtccccgac gacgccatcg
     3361 tcgccgtcgc ggagtccatc cgcctgtacg cgggtcaggc gctgatccac accagcggcg
     3421 tcctcgaggc cgaggcgctc cgcccggcga tggccgcggg cacccaggcc ggcgcgttcc
     3481 acccgttggt cgccttcgcc gacctggagc gcgcgctggc cgcgctgccc ggcgcgacga
     3541 tcgccatcga aggcgacgac gagctggccg cgcacctggg ggacatggcc gaggccatcg
     3601 gtggcctgcc ggtccgcctg ccgccgggga cgaaggccgc ctaccacgcg gccgccgtgc
     3661 tggccgccgg cggggtggtg gccctcctgg acacggtccg cgagatcgcc gggctgctgg
     3721 gcctcgatga ggcgggggcg atggacgtct atctgccact cctggagcag gctgtggcaa
     3781 acgcccgagc actcgggatt gccgcgtcgc tcaccggacc cgcgacgcgc ggcgacatcg
     3841 ggaccatcgg cgcccacctc gccgcgctcg aggcgggcgc cccggacgcg cttcccgtgt
     3901 accgggcgct gctgacccgg ggcgcgggca tcgccctcga ccggggcgcg ttgtcaccgg
     3961 agtctgccga gcggctgagc gccgcacttg caggcaaccg gtgaagcggt aggatgccgc
     4021 catgcagcac agcatcgccg cccgcttcgc ggcctggcca gcgcgcttcg tccacccggc
     4081 ggcgtcggct cgtgagcggc gcctcggcct gagacccgcg tcggcgttca tcgccgcctc
     4141 cggtcgccgg actgtgctcc gccggcccga cgtcgggccc ctgacggccc tccgcgcgcc
     4201 gtggcgtgcg ctcacgatcg aaccggtgcg ggagcgcgtc agcggccggc cggagcctgc
     4261 gcagcggatg cggtggacga gctccacccg ggccggggga tcgcgccgct cctccgtggc
     4321 gcccgcgtgg cccgcctgag gcaggccaca gccacctctg caggtggcat cgtcatccgg
     4381 tccaccggcg gcacccccga actcgtggtc ggcatgcgga gacgggaacg cgacggcgtc
     4441 acctggacgc tgcccaaggg cacgccgatc ccgaacgaga cgaccgagca gaccgccgtg
     4501 cgcgaggtcc gcgaggagac gggtctcgag gtccggatca tcagcccgtt cgaccacatc
     4561 gactacacgt tcgtgatggg tgggacgcgc atctcgaaga ccgtccacta cttcctgatg
     4621 gt
//
LOCUS       DBNHXO010000342         2974 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1488374, whole genome shotgun sequence.
ACCESSION   DBNHXO010000342 DBNHXO010000000
VERSION     DBNHXO010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1488374"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..545
                     /locus_tag="AC162A_07120"
     CDS             174..545
                     /locus_tag="AC162A_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010650.1"
                     /translation="MTAGSVDGTLDLNQAVRRWIALGGWLSAFGYSASTSGISGTLLL
                     LWSLVLLISTVTSPTKQGLHDRFANSAIVAPASGSMGGIALACVLIVGAIVLIALVAI
                     VALILLGGQINSILSAAGESI"
     gene            571..2103
                     /locus_tag="AC162A_07125"
     CDS             571..2103
                     /locus_tag="AC162A_07125"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR5010651.1"
                     /translation="MSDLGEPGLDPQQVDGRNDMDPEAFRAAAHDVVDRIADYLATIE
                     QRPVLPPIEPGSIAPLFPAHAPDRPEPLEAILADVDRLVVPNATHWQHPGFLAYFATT
                     ASGPGMLGEFLTAALGQNPMLWRTSPIGTELETVVVGWLREALGLPDTFSGLLTDTAS
                     TSSLIALAAAREASGLDAAARGLAARAKGGDPRVYASAEAHSSIEKACMTLGLGRAAV
                     VKVPANDAYEMDVDALATAIETDHLAGRCPVAVVATIGTTSSTSVDPVSAIADVAARE
                     GLWLHVDAAYAGPVALLPERRSAFVGWDRADSIVVNPHKWLFTPLDASLLLTRRMDQL
                     RAAFGLVPEYLRTLDRASPVLDLNEYQPQLGRRMRALKLWMLIRWFGLDGLRRRIDFH
                     LELAARFAAWVDADPDWERLAPVPFSTVCFRYRPAALTGQEDEGDVPDLLDDLNARLM
                     DAVNRTGDVFLSHTRLRNRFTIRLAVGNLRTEERHVERAWELLRREAARLATETPRGS
                     DA"
     gene            2100..2459
                     /locus_tag="AC162A_07130"
     CDS             2100..2459
                     /locus_tag="AC162A_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010652.1"
                     /translation="MTEPVEILYFYRTRWGAHDEFVALFEKNHWPILREQLASGRFTD
                     VQMWTPRFHGEGRADWDVLVSITYRDWAAVEAHGDAEFARRLYPDQETFHAEERHRFA
                     LLDAHWDVVLESRSLER"
     gene            complement(2473..>2974)
                     /locus_tag="AC162A_07135"
     CDS             complement(2473..>2974)
                     /locus_tag="AC162A_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010653.1"
                     /translation="AVATESCMSATHAHASQVDRDLDLDGRSPGAPRPDDAGRSPAPI
                     AGTADPRFAGGVSAPNLMALQRSAGNAAVASLVAPRSVQRAVQIGEVTTSVDAVDSPP
                     ETAGAGGAGASGAVTSDGGTTTITGAVINLDSAMTQTSGVIRAGTIIADSVVAASYSP
                     GAGNVW"
BASE COUNT          392 a         1069 c         1058 g          455 t
ORIGIN      
        1 ccgcacgtat cgccgcttgc cgcgggtcaa tgacgcggac ctcaatcaga agaacgctca
       61 cgcggtcgca atgtttcaca acggccgtca cgaatcgggg accgggacgt tgacagcggt
      121 agcaccgtgt cgcggaccgt ccctcgatgg ggcggcgtcg agggtcgatc gtgatgacag
      181 ccggctcggt ggacggcacg ctcgacctca accaggccgt ccggcgttgg atcgcactgg
      241 gtggctggct ctcggccttc ggctactccg cgagcacgtc ggggatctcc gggaccctgc
      301 tgctcctatg gtcgctcgtg ttgctgatct cgaccgtcac gagcccgacg aagcaaggcc
      361 tccacgaccg gtttgccaac tcggcgatcg tcgccccggc gagcggctcc atgggcggca
      421 tcgcgctcgc ctgcgtgctg attgtcggcg ccattgtcct catcgccttg gtagcgatcg
      481 tcgccctgat cctactcggc ggccagatca actcgatcct ctcggccgcc ggcgaatcga
      541 tctgaccgac tgccccggta gactggccgg gtgagcgacc tgggggagcc gggcctcgac
      601 ccgcagcagg tcgatggccg caacgacatg gacccggagg cgttccgcgc ggcggcgcac
      661 gacgtggtcg accggatcgc cgactacctc gccaccatcg agcagcgtcc ggtcctgccc
      721 ccgatcgagc ccgggtcgat cgcgccgctg ttcccggccc acgcgcccga ccggccggaa
      781 cccctcgagg ccatcctggc cgacgtcgat cgtctcgtcg tccccaacgc cacccactgg
      841 cagcacccgg gcttcctcgc gtacttcgcg acgactgcct cgggtcccgg gatgctgggc
      901 gagttcctca cggccgcgct cgggcagaac ccgatgctgt ggcggacgtc gccgatcggc
      961 acggagctcg agaccgtcgt ggtcggctgg ctgcgcgaag ccctcggcct gcccgacacc
     1021 ttcagcggcc tgctcaccga cacggcctcg acgtcatcgc tgatcgccct ggccgcggcc
     1081 cgtgaggcgt cgggcctcga cgccgcggca cgcgggctcg cggcgcgggc gaagggcggc
     1141 gatccgcgcg tctacgcgtc ggccgaggcc cacagctcga tcgagaaggc atgcatgacg
     1201 ctggggctgg ggcgcgcggc cgtggtcaag gtcccggcca acgacgccta cgagatggac
     1261 gtcgatgcgc tcgcgacggc gatcgagacg gatcacctcg ccggccgctg cccggtggcg
     1321 gtggtcgcca ccatcggcac gacctcgtcg acgtcggtcg acccggtgtc ggcgatcgcg
     1381 gacgtcgccg cacgcgaagg cctctggctg catgtcgacg ccgcctatgc cggacccgtc
     1441 gcgctgctgc ccgagcgtcg atcggcgttc gtgggctggg accgcgccga ctcgatcgtc
     1501 gtcaacccgc acaagtggct gttcaccccg ctcgacgcgt cgctcctgct gacgcggcgg
     1561 atggaccagc tgcgggcggc gttcggtctc gtgcccgagt acctgcggac cctcgaccgc
     1621 gcgtcgccgg tgctcgacct gaacgagtac cagccgcagc tcggccggcg catgcgcgcg
     1681 ctgaagctgt ggatgctgat ccgctggttc gggctcgacg ggctgcggcg gcggatcgac
     1741 ttccacctgg agctcgctgc gcggtttgcc gcctgggtgg acgcggatcc tgattgggag
     1801 cgcctggcgc cggtgccgtt ctcgacggtg tgcttccgct atcgcccggc ggcgctgacc
     1861 ggtcaggagg acgagggcga cgtgcccgac ctgctcgacg acctcaacgc ccggctgatg
     1921 gacgcggtca accgcacggg cgacgtgttc ctgtcccaca cccggctgcg taaccggttc
     1981 acgatccgcc tggccgtcgg caacctgcgc acggaggagc ggcacgtcga gcgtgcgtgg
     2041 gagctcctgc ggcgcgaggc cgcgcggctg gccaccgaga cgccacgagg gagcgacgca
     2101 tgaccgagcc ggtcgagatc ctgtacttct accgcacccg ctggggggcc cacgacgagt
     2161 tcgtggcgct gttcgagaag aaccactggc cgatcctgcg cgagcagctc gcgtcgggcc
     2221 ggttcaccga cgtccagatg tggacgccgc ggttccacgg ggagggccgg gcggactggg
     2281 acgtgctggt ctcgatcacg tatcgcgact gggcggcggt cgaggcgcac ggtgacgccg
     2341 agttcgcgcg gcgtctctac ccggaccagg aaacgttcca cgcggaggag cgccaccggt
     2401 tcgcgttgct ggatgcgcac tgggacgtcg tgctggagtc gcgctcgctg gagcgctagg
     2461 ccgacctcac cctcaccaca cgttgccggc gccgggcgag tagctcgcgg cgacgaccga
     2521 gtcggcgatg atcgtgccgg cgcggatcac gccgctcgtc tgggtcatcg cggagtccag
     2581 gttgatcacc gccccggtga tcgtcgtggt gccgccgtcc gacgtcacgg cgccgctcgc
     2641 gccggcgcca cccgccccgg cggtctcggg cgggctgtcg acggcatcga cgctggtcgt
     2701 gacctcgccg atctggacgg ctcgctgcac ggacctcggc gcgaccagcg acgcgaccgc
     2761 cgcgttcccg gccgatcgct ggagggccat cagattgggc gccgagaccc cgccggcgaa
     2821 ccgcgggtcc gccgttccgg cgatgggcgc cggtgatcgg ccggcgtcgt ccggccgcgg
     2881 cgcgccaggc gagcgcccat cgaggtcgag atcgcggtcg acctggctcg cgtgggcgtg
     2941 ggtcgcggac atgcagctct ccgttgcgac ggcc
//
LOCUS       DBNHXO010000343         1823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3074287, whole genome shotgun sequence.
ACCESSION   DBNHXO010000343 DBNHXO010000000
VERSION     DBNHXO010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3074287"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..1550)
                     /locus_tag="AC162A_07140"
     CDS             complement(273..1550)
                     /locus_tag="AC162A_07140"
                     /inference="COORDINATES: protein motif:HMM:NF017984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5010654.1"
                     /translation="MNHKPRTVSAAVARRFLVTRHLLAPPRSLPAEPASVMRVIDRLG
                     SLQFDPLEVAGRNHDLVLLARIDGYRRPWTDDLLYRDRVLYETYNKMFSIVPTAELPW
                     YRITWDRNHEAHRGGAFDDHAPLVEELLARIRAEGALSSTDVEPRAAIDWYWRPTNQV
                     RALLEALAEAGILAIARREGNRRVYDLTERLFPPDLLGRQVPTDEQLRHKLLSRHRGN
                     GLLGATGDYTLWMGMGKAPERRRVHDELVESGAITAVAVAGMRGERFVVTDELPMLDA
                     AEGEVETEDRLGAPPQPAGSEPGVAFLAPLDPLAWDRDLLLRLFDFDYRWEVYVPAAK
                     RRWGYYVLPLLFGDRLVGRIEPRIDRAKGTLRVLGLWWEDGFDPLDVRHPGFADAFAA
                     ALRAHASFAGLSKVTLPRVARHQAVARAVRERL"
     gene            complement(1588..>1823)
                     /locus_tag="AC162A_07145"
     CDS             complement(1588..>1823)
                     /locus_tag="AC162A_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR5010655.1"
                     /translation="APIVYGRALSLHPAIVLIAVPVGGAVAGILGMFLVVPIVAIVSA
                     TWRLAVATIEDDSPAGASAAPAAEAEATATAGT"
BASE COUNT          270 a          649 c          666 g          238 t
ORIGIN      
        1 tgcccaacca ctcacggggt gaatgcatgg ccaactccgg agcgtccttg ttggcccagc
       61 attcgcgtcg tgaatatgtg cgcaggtcgc ggaccgatcc ggccccttcc cattcactgc
      121 atgagtaggt ggcagggtcg cccgggagcg gctcgcgttt gcattcacca cgtggctata
      181 gggccgggtc gccaggtggg gcggatcggt cgggcggacg cggacgggcc cgcggctacg
      241 gtcgactgac gaggacgcgc gaccgcgacg cgctacagcc gctcgcggac cgctcgtgcg
      301 acggcctgat gccgagccac ccgcgggagc gtcaccttcg acagccccgc gaacgacgcg
      361 tgggcgcgca gcgcggccgc gaacgcgtcc gcgaacccgg gatgacgaac gtccagcggg
      421 tcgaacccgt cctcccacca cagcccgagc acccgcagcg tgcccttggc ccggtcgatc
      481 cggggctcga tgcggccaac cagccggtcg ccgaacagca gcggcaggac gtagtagccc
      541 cagcgccgct tggccgcggg cacatagacc tcccagcggt agtcgaagtc gaacagccgg
      601 agcagaaggt cgcggtccca ggccagcggg tcgagcgggg cgaggaacgc gaccccgggc
      661 tccgagccgg ccggctgcgg cggggccccg agccggtctt ccgtctcgac ctcgccctcg
      721 gccgcgtcga gcatcggcag ctcgtccgtc actacgaacc gctcgccgcg catgcccgcg
      781 acggcgacgg cggtgatcgc cccggactcg accagctcgt cgtggacgcg acgccgctcg
      841 ggggccttcc ccatgcccat ccacagcgtg tagtcgccgg tcgcgccgag gagcccgttg
      901 ccgcgatgtc gtgacagcag cttgtgccgc agctgctcgt cggtcggcac ctggcgaccc
      961 aggagatcgg gcgggaacag gcgctcggtc aggtcgtaga cgcgccggtt gccctcacgg
     1021 cgcgcgatgg ccaggatgcc ggcctcggcg agcgcctcga gcagggcccg gacctggttg
     1081 gtcggccgcc agtaccagtc gatcgcggcg cgcggctcca cgtcggtcga cgacagagcg
     1141 ccctcggccc ggatccgcgc cagcagctcc tcgacgagcg gcgcgtgatc gtcgaacgcc
     1201 ccgccgcggt gcgcctcgtg gttccgatcc caggtgatcc ggtaccacgg cagctcggcg
     1261 gtgggcacga tcgagaacat cttgttgtac gtctcgtaga gcacccgatc gcggtacagg
     1321 agatcgtcgg tccaggggcg ccggtagccg tcgatgcggg cgaggagcac caggtcgtgg
     1381 ttgcggccgg ccacctcgag cgggtcgaac tggagcgagc ccagccggtc gatgacgcgc
     1441 atgaccgagg ccggctcggc gggcagcgag cgcggcgggg cgaggagatg gcgggtgacg
     1501 aggaaccgcc gcgcgacggc ggcggacacg gttcggggct tgtggttcac gggccttgat
     1561 cgtacggtcg agcgtgaccc ggacctgtca tgttcccgcg gtggccgtcg cctcggcttc
     1621 cgccgccggg gccgcggacg cacccgcggg gctgtcgtcc tcgatcgtcg cgaccgcgag
     1681 ccgccacgtt gccgacacga tcgccacgat cggcaccacg aggaacatgc ccaggatgcc
     1741 ggcgaccgca cccccgacag ggaccgcgat cagcacgatc gccgggtgga ggctcagggc
     1801 gcgcccatag acgatcgggg cca
//
LOCUS       DBNHXO010000344         3549 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3074310, whole genome shotgun sequence.
ACCESSION   DBNHXO010000344 DBNHXO010000000
VERSION     DBNHXO010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3074310"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="AC162A_07150"
     CDS             complement(<1..393)
                     /locus_tag="AC162A_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR5010656.1"
                     /translation="MFEALLNAFRAPDLRRRILFVAGILLVFRGLAHVPIPGVNAAQL
                     QDFLSTNAIFGLLDLFSGGGLSNFSVVALGVNPYINASIIMQLMTGVVPSLQALQREG
                     EYGRTKINQYTRYLSVPMALLQAYGLLAL"
     gene            complement(669..1130)
                     /gene="rplO"
                     /locus_tag="AC162A_07155"
                     /pseudo
     CDS             complement(669..1130)
                     /gene="rplO"
                     /locus_tag="AC162A_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006895589.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            complement(1130..1348)
                     /gene="rpmD"
                     /locus_tag="AC162A_07160"
     CDS             complement(1130..1348)
                     /gene="rpmD"
                     /locus_tag="AC162A_07160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR5010657.1"
                     /translation="MPGRLRVTQTRSTISHIARNRATIRALGLHGIGSSSTLPDNPAV
                     RGMVRQVRFLVTVEELPAGADATTPEKH"
     gene            complement(1338..1886)
                     /gene="rpsE"
                     /locus_tag="AC162A_07165"
     CDS             complement(1338..1886)
                     /gene="rpsE"
                     /locus_tag="AC162A_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR5010658.1"
                     /translation="MARIDPNKLALEERVVQINRVAKVHKGGRRFSFSAVIVVGDGNG
                     HVGVGLGKAGDVPESIRKGVEDAKKNIVRIPMVGTTIPHEVRVEYSAARVMLKPASQG
                     TGVIAGGAVRAVVEAAGIRDILAKVHGSTNPVNVTRATLNALSSLNSVEELSRRRGIK
                     LHLVVPGQPAASLSRREAADAR"
     gene            complement(1898..2260)
                     /gene="rplR"
                     /locus_tag="AC162A_07170"
     CDS             complement(1898..2260)
                     /gene="rplR"
                     /locus_tag="AC162A_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR5010659.1"
                     /translation="MAHVATRSSARTKRHERLRLRLAGTTNRPRLAVFRSLNHIYAQV
                     IDDTNGHTVAAASSLESGLRGSDGSKTDDAKRVGKLVAERAKAAGVEQVVFDRAGFKY
                     HGRVRSLAEAAREAGLDF"
     gene            complement(2263..2814)
                     /gene="rplF"
                     /locus_tag="AC162A_07175"
     CDS             complement(2263..2814)
                     /gene="rplF"
                     /locus_tag="AC162A_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR5010660.1"
                     /translation="MSRIGRLPITVPAGVDVSFDGRIITVKGPKGTLTRELPPRIEVA
                     QSDGTIVVTRPTENKLDKSLHGLTRTLVNNMVVGVTTGYRKGLEIVGVGYRAQKVGDK
                     LQLALGYSHPIEIDPPAGISFEVETPLRLSVVGIDKELVGQVAAKVRATRKPEPYKGK
                     GVRYAGEQVRRKAGKAGKIGGKK"
     gene            complement(2821..3219)
                     /gene="rpsH"
                     /locus_tag="AC162A_07180"
     CDS             complement(2821..3219)
                     /gene="rpsH"
                     /locus_tag="AC162A_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR5010661.1"
                     /translation="MNVSDPIADMLTRVRNASRARHTEVIVPASRTKREIARILKEEG
                     FIADVHEEREGVAQLLRIELKYVDGKVPVVSGLKRISKPGLRVYARKTDIPRVLGGLG
                     VVIVSTSRGIMTGSQAKQAQLGGEILAYIW"
     gene            complement(3224..3409)
                     /locus_tag="AC162A_07185"
     CDS             complement(3224..3409)
                     /locus_tag="AC162A_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIR5010662.1"
                     /translation="MAKKSMIAKAKRTPHHPVQAYHRCFVCGRPRAYMRRFALCRICF
                     RERALVGELPGVTKSSW"
     gene            complement(3427..>3549)
                     /gene="rplE"
                     /locus_tag="AC162A_07190"
     CDS             complement(3427..>3549)
                     /gene="rplE"
                     /locus_tag="AC162A_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995900.1"
                     /note="part of 50S and 5S/L5/L18/L25 subcomplex; contacts
                     5S rRNA and P site tRNA; forms a bridge to the 30S subunit
                     in the ribosome by binding to S13; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR5010663.1"
                     /translation="EIDYDKVDRLRGLEISIVTTAKTDEESKRLLELLGMPFAG"
BASE COUNT          465 a         1205 c         1300 g          579 t
ORIGIN      
        1 gagcgccagg agcccgtagg cctggagcag cgccatcggc acggacaggt agcgcgtgta
       61 ctggttgatc ttggtgcggc cgtactcgcc ctcgcgctgc agcgcctgca gcgacgggac
      121 gacgcccgtc atcagctgca tgatgatcga ggcgttgatg tacgggttca cgcccagcgc
      181 cacgaccgag aagttcgaca ggccgccgcc cgagaagagg tcgagcagcc cgaagatcgc
      241 gttggtgctg aggaagtcct ggagctgcgc cgcgttgacg cccgggatcg gaacgtgggc
      301 gagcccccgg aagaccagca ggatcccggc gacgaacagg atgcgccgcc ggaggtccgg
      361 cgcacggaac gcgttgagca gcgcctcgaa cacgggcgtt agccgttgtc ggccgcttcg
      421 gcctcgtcgg cctccgcggg cgtcccgggc tcggtggcgt cctcgggggt ggcggcgtcg
      481 gcggccggct cgacgaccgc ctcggcggca gcctcgtccg cgacgtcgcc ggcggtggtc
      541 tcgtcaacgt cggcggcggc cttggcggcc ttgggggcgc gacgcgcctt gggcgccgga
      601 gccggctggt cggccggaac gtcgacgccg agcgccgcga gcgggctggt cgggatctcg
      661 atgacctggg cggtgccgcc cgcggcctcg atcttctccc gcgcgctgcg cgtgaacgcg
      721 tcggcgagga cgaacagctt cgcgctcacg tcgccgttgc ccaggatctt gagcggcagc
      781 cggtcgcgac ggaccaggcc cgcgcccttg agcgtgtcgg cgttgaccgt gaccggcgcg
      841 cccttctgga cctcgagctt gccggcctcg atcgccgcgc tgatcgcgcc gacgttgacg
      901 acctcgtact cgatgtcgtt gatgttgcgg aacccgcgca gcttcggaat tcggatgtgg
      961 aggggggtct gcccgccctc gaaccaggcc gggatcgagg cgcccgcgcg cgagcgctgg
     1021 cccttcgtgc cgcggccggc ggtcttgccc ttgccggcgg cgatgccgcg gccgacccgg
     1081 gtccgcttgg tgaccgaccc ctcggcgggg cgcaggtcgt ggatcttcat cagtgcttct
     1141 ccggggtcgt cgcgtccgcg cccgccggga gctcctcgac ggtcacgagg aagcggacct
     1201 ggcggaccat cccgcgcacg gcgggattgt cgggcagggt cgacgagctg ccgatcccgt
     1261 gcaggccgag ggcgcggatc gtcgcccggt tgcgggcgat gtggctgatg gtgctcctgg
     1321 tctgggtgac gcgcagccta ccgggcatcg gcagcctccc gccgcgacag ggacgcggcc
     1381 ggctggccgg ggacgaccag gtgcagcttg atgccccggc ggcgcgacag ctcctcgacc
     1441 gagttgaggc tgctcagggc gttgagcgtc gcccgggtca cgttcaccgg gttggtggag
     1501 ccgtggacct tggccaggat gtcgcggatg ccggccgcct cgacgacggc acgcacggcg
     1561 ccaccggcga tgacgccggt gccctgcgag gccggcttga gcatcacccg cgcggcggag
     1621 tactccacgc ggacctcgtg cgggatcgtg gtcccgacca tggggatccg gacgatgttc
     1681 ttcttcgcgt cctcgacgcc cttccggatg gactccggga cgtcgccggc cttgcccagg
     1741 cccacgccga catggccgtt gccgtcgccc acgacgatga ccgccgagaa cgagaagcgg
     1801 cggccgccct tgtggacctt ggcgacgcgg ttgatctgga cgacgcgctc ctcgagcgcg
     1861 agcttgttgg gatcgatgcg ggccacaggc gtgtcctcta gaagtcgaga ccggcctcgc
     1921 gggccgcctc ggcgagcgag cgaacccgcc cgtggtactt gaagcccgcc cggtcgaaca
     1981 ccacctgctc gacgccggcg gccttggcgc gctcggcgac gagcttgccg acgcgcttcg
     2041 cgtcgtcggt cttcgacccg tcggaaccac gcaggccgga ctcgagcgac gaggccgcgg
     2101 ccacggtgtg gccgttggtg tcgtcgatga cctgcgcgta gatgtgattc aggctccgga
     2161 acacggcgag ccgcgggcgg ttcgtcgtgc cggcgaggcg gaggcggagg cgctcatgac
     2221 gcttggtgcg cgccgacgag cgggtggcaa cgtgggccat ggctacttct tcccgccgat
     2281 cttgccggcc ttgcccgcct tgcgacggac ctgttcaccg gcgtagcgga cgcccttgcc
     2341 cttgtacggc tcgggcttgc gggtcgcgcg gaccttggcg gccacctggc cgacgagctc
     2401 cttgtcgatg cccaccaccg acaggcggag gggggtctcg acctcgaagc tgatgccggc
     2461 cggcgggtcg atctcgatcg ggtggctgta gccgagcgcg agctggagct tgtcgcccac
     2521 cttctgcgcg cggtagccca cgccgacgat ctcgaggccc ttgcggtacc cggtcgtcac
     2581 gccgacgacc atgttgttga cgagggtccg ggtcagcccg tggaggctct tgtcgagctt
     2641 gttctccgtc ggccgggtga ccacgatggt gccgtccgac tgcgcgacct cgattcgggg
     2701 cggcagctca cgcgtgaggg tgcccttggg gcccttcacg gtgatgatcc gcccgtcgaa
     2761 cgagacgtcg acgccggcgg ggacggtgat ggggagacga ccgatacggg acatcgtgcg
     2821 ttaccagatg taggcgagga tctcgccacc cagctgtgcc tgcttggcct gcgagccggt
     2881 catgatcccg cgcgaggtgc tgacgatcac gacgccgagg cccccaagga cgcgcgggat
     2941 gtcggtcttg cgcgcataga cacgcaggcc gggcttgctg atccgcttga gtccggagac
     3001 gaccggcacc ttgccgtcga cgtacttgag ctcgatgcgc aggagctggg ccacgccctc
     3061 gcgctcctcg tggacgtcgg cgatgaagcc ttcctccttg aggatgcgcg cgatctcgcg
     3121 cttggtgcgc gaggcgggca cgatcacctc ggtgtggcgc gcccgggacg cgttgcggac
     3181 gcgcgtgagc atgtccgcga tcgggtcgga gacgttcatc ttcttaccag ctggacttcg
     3241 tgacgccggg cagctcgccg acgagcgctc gctcgcggaa gcagatccgg cacagcgcga
     3301 atcggcgcat gtacgcccgc ggccgcccgc agacgaagca gcggtggtac gcctgcaccg
     3361 gatggtgcgg ggtgcgcttt gccttcgcga tcatggactt cttggccatt ccgactcctc
     3421 ccgcgcctag ccggcgaagg gcatgccgag gagttcgagc agacgcttcg actcctcgtc
     3481 ggtcttcgcc gtcgtcacga tgctgatctc cagcccgcgg agacggtcca ccttgtcgta
     3541 gtcgatctc
//
LOCUS       DBNHXO010000345         2136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4265591, whole genome shotgun sequence.
ACCESSION   DBNHXO010000345 DBNHXO010000000
VERSION     DBNHXO010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4265591"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="AC162A_07195"
     CDS             <1..412
                     /locus_tag="AC162A_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR5010664.1"
                     /translation="GLLDTSTLLLLPRLDAERDLPRTPLISAVTLAELAVGPLVATEV
                     AERATRIAQVQQAEADFQPIPFDVAAARAFGPVAADLRASGRKTTARAFDAMIAAIAV
                     ANGLPLYTCNASDFAGIEGLEVVAVAHPDRHPRR"
     gene            438..1457
                     /gene="trpS"
                     /locus_tag="AC162A_07200"
     CDS             438..1457
                     /gene="trpS"
                     /locus_tag="AC162A_07200"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR5010665.1"
                     /translation="MTVEIAPSPAPAASPSRRPRIFSGIQPSGIVHLGNDLGAIRNYV
                     MLQFEYEAIYCIVDYHALTSTHDPDLMRTRTREMAAGLLALGLDPERCTLFVQSHRPE
                     HTELMWLLATVTPVSWLERTPTYKEKKLNQPDDINHGLLTYPVLQAADIVIYKASLVP
                     VGKDQAAHLELSREIVRAFNHRYGETFPEPEAVFTQAPVVLGTDGVKKMSKSVGNTID
                     ILGEPDLIRKQVVSMVTDTKRILRTDPGRPEVCNVCQLHRFFGDDFEEVWDGERTART
                     GCVDTKKLLADRIIRHYAPARERYAELMAHPAEVDGILEAGADRLKPKAEATMAEVRQ
                     KMGLR"
     gene            1454..1960
                     /locus_tag="AC162A_07205"
     CDS             1454..1960
                     /locus_tag="AC162A_07205"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR5010666.1"
                     /translation="MTTGRVEAIRIARIGGAPMASVERVAAHAGGGLEGDRYHKGTGE
                     WSSSPGAGRGLTIISADTLDEANRENPGLDIAAVDTRRNVEVRGVDLDDLVGREFTIG
                     TARCVAIRLAEPCTYLEGLVGRPILRALSHKAGIRADILEDGEIAVGDAIVPGVRIAA
                     PAEVAAPH"
BASE COUNT          328 a          764 c          751 g          293 t
ORIGIN      
        1 cggcctgctc gataccagca cgctgctgct gctgccgcgg ctcgacgccg agcgggacct
       61 cccgcgcacg cccctcatct cggccgtgac gctggccgag ctcgccgtgg gcccgctcgt
      121 ggcgaccgag gtcgccgagc gggccacgcg gatcgctcag gtccagcagg cggaggcgga
      181 tttccagccg atccccttcg atgtcgccgc ggcacgagcg ttcgggccgg tagccgcgga
      241 cctgcgcgcg tcgggacgca agacgacggc ccgggcgttt gacgcgatga tcgcggcgat
      301 cgccgtcgcc aacgggctcc cgctgtacac ctgcaacgcg tccgacttcg cgggcatcga
      361 gggtctcgag gtcgtcgcgg ttgcgcatcc ggatcggcac ccgagacgct gaaggcgctc
      421 cgctacactc cggcgcgatg accgtcgaga tcgccccctc gcccgcgcca gccgcgtcgc
      481 cctcgcgccg gccgcgcatc ttcagcggga tccagccgtc ggggatcgtc cacctgggca
      541 acgacctggg cgcgatccgg aactacgtga tgctccagtt cgagtacgag gcgatctact
      601 gcatcgtcga ctaccacgcg ctcacgagca cccacgaccc cgacctcatg cggacgcgca
      661 cgcgcgagat ggcggccggc ctgctcgccc tgggcctgga ccccgagcgc tgcaccctgt
      721 tcgtccagag ccatcggccc gagcacaccg agctgatgtg gctgctggcg acggtgacgc
      781 cggtgagctg gctcgaacgg acgccgacgt acaaggagaa gaaactcaac cagcccgacg
      841 acatcaacca cgggctgctc acatatcccg tgctccaggc cgccgacatc gtcatctaca
      901 aggcttcgct ggtgccggtc ggcaaggacc aggcggccca cctcgagctg tcgcgcgaga
      961 tcgtccgcgc gttcaaccac cgctacggcg agacgttccc cgagcccgag gcggtgttca
     1021 cgcaggcgcc ggtggtcctc ggcaccgacg gcgtcaagaa gatgtccaag tcggtcggca
     1081 acaccatcga catcctgggc gagccggacc tgatccggaa gcaggttgtc tcgatggtca
     1141 ccgacaccaa gcggatcctg cgcacggacc ccggccgacc cgaggtgtgc aacgtgtgcc
     1201 agctccaccg cttcttcggc gacgacttcg aggaggtctg ggacggcgag cggacggcgc
     1261 ggaccggctg cgtcgatacg aagaagctgc tggccgaccg gatcatcagg cactacgccc
     1321 cggcccgcga gcgctacgct gagctgatgg cgcaccccgc cgaggtcgac gggatcctgg
     1381 aggccggcgc ggaccggctc aagccgaagg cggaggcgac gatggccgag gtgcggcaga
     1441 agatggggtt gcgatgacga cgggacgggt ggaagcgatc cggatcgcgc gaatcggcgg
     1501 ggcgccgatg gcctcggtcg agcgcgtcgc cgcgcatgcg ggcggcgggc tcgagggcga
     1561 ccgctatcac aagggcaccg gtgagtggtc gagctcgccg ggcgcgggcc gcggcctgac
     1621 gatcatctcc gcggacaccc tggacgaggc gaaccgcgag aacccggggc tcgacatcgc
     1681 ggcggttgac acgcgccgga acgtcgaggt gcgtggcgtc gacctggacg acctcgtcgg
     1741 gcgcgagttc acgatcggga cggcgcgctg cgtcgccatc cgcctcgcgg agccgtgcac
     1801 ctacctcgag ggcctcgtcg gccggccgat cctgcgcgcc ctgtcccaca aggcagggat
     1861 cagggcggac atcctcgagg acggcgagat cgccgtgggc gacgcgatcg tgcccggcgt
     1921 ccgcatcgcg gcgcccgcgg aggtcgccgc gcctcactga cctccgggct gtcgagcgcc
     1981 cacccgtcgg cgtgtcccgg acaagtcgcg gcgtgtaggc tgacgccatg cccgacggcc
     2041 tgacggaacg ccagcggtgg ctgatcgacg ccgcgctcgt cctcgcggtg atcgccctcg
     2101 ggttcgtcgt gctcgggttc gccgccaacg tgttct
//
LOCUS       DBNHXO010000346         1938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_694276, whole genome shotgun sequence.
ACCESSION   DBNHXO010000346 DBNHXO010000000
VERSION     DBNHXO010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_694276"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1938
                     /locus_tag="AC162A_07210"
     CDS             <1..>1938
                     /locus_tag="AC162A_07210"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR5010667.1"
                     /translation="FVIYDTDDQQQLMKRILAEEDLPATGEFRPAAILGAISRAKNDM
                     LDEAFLAENAVNHRERIIARLAGRYRDRLRAANALDFDDLLLRAVELFEQSPDTLAKY
                     HARWRYLHVDEYQDTNRPQYLWIRALASASRNLCVVGDDDQSIYGWRGANVQNILDFE
                     RDYPEATVVKLEQNYRSTQLILDAAHAVVSRNERRSDKKLWTENQGGVPIQRFEAFNE
                     EEEAEWIARQIDGLVGSSGRGSWLTRRADDDEESSRFRAKDVAVMYRMNAQSRAIEEK
                     FLTYGIRYQIVGGTRFYQRREVKDALAYLRVLRSDTDVVSFERIINVPARGIGDKSVE
                     ALRRWAVAHEDNTSAAVQAAAAGEVAGLAPRIRTAMGEFARLLIRLRTRIGVLPLPEL
                     LDEVLEASGYRAMLADGSEEGEDRWANLLELRQVTTRYDDLSPDDALDRLLEETALVA
                     DQDAYEGDADAVTLITLHAAKGLEFPVVFIAGLEEGLFPHNRALDDEKELEEERRLAY
                     VGITRAKRRLYLSHAWRRATWGGGGMSVPSRFLLEIPAELMVGPQLQRGADYGVDIDP
                     DLVFGDRRSRFGQAIRSGGGAYREGSGRPGAPAAGEAFRPSRDLGAKREAFAAGAPSG
                     SLGPQPRSTPSVPARPIVPGER"
BASE COUNT          299 a          674 c          710 g          255 t
ORIGIN      
        1 ttcgtgatct acgacaccga cgatcagcag cagctgatga agcggatcct ggccgaggag
       61 gacctgccgg cgaccggcga gttccgccct gcggcgatcc tgggggcgat cagccgggcc
      121 aagaacgaca tgctcgacga ggcgttcctc gcggagaacg cagtcaacca ccgggagcgg
      181 atcatcgccc gcctcgccgg gcgctatcgg gaccgcctgc gcgccgccaa cgcgctcgat
      241 ttcgacgacc tcctgctgcg cgccgtcgag ctgttcgagc agtcgcccga cacgctcgcg
      301 aagtaccacg cacgctggcg ctacctccac gtggacgagt accaggacac gaaccggccg
      361 cagtacctgt ggatccgggc gctcgcgtcg gcctcgcgga acctgtgcgt ggtcggcgac
      421 gacgaccagt cgatctacgg ctggcgaggc gcgaacgtcc agaacatcct cgatttcgag
      481 cgcgactatc ccgaagcgac ggtcgtgaag ctcgagcaga actaccgctc gacccagctg
      541 atcctcgacg cggcgcacgc ggtcgtgtcg cgcaacgagc gccgctcgga caagaagctg
      601 tggacggaga accagggcgg cgtcccgatc cagcgcttcg aggccttcaa cgaggaggag
      661 gaggccgagt ggatcgcccg ccagatcgac ggcctcgtgg gatccagcgg ccggggctcg
      721 tggctcacgc ggcgggccga cgacgacgag gagtccagcc ggttccgggc caaggacgtc
      781 gccgtgatgt accggatgaa cgcccagtcc cgcgcgatcg aggaaaagtt cctcacctac
      841 ggcatccgct accagatcgt gggcggcacg cgcttctacc agcggcgcga ggtgaaggac
      901 gccctggcct acttgcgggt gctccgctcc gacacggacg tggtctcgtt cgagcgcatc
      961 atcaacgtgc cggcgcgcgg catcggtgac aagagcgtcg aggcgctgcg acgctgggcc
     1021 gtcgcccacg aggacaacac ctcggcggcc gtccaggccg cggccgcggg cgaggtcgcc
     1081 gggctggcgc cacggatccg gacggcgatg ggggagttcg ccaggctcct gatccgcctc
     1141 cgcacccgga tcggcgtgct gccgctcccc gagctgctcg atgaggtcct cgaggcgtcg
     1201 ggctaccggg cgatgctcgc cgacggctcg gaggagggtg aggaccgctg ggcgaacctg
     1261 ctcgagctgc gccaggtcac gacccgctac gacgacctgt cgcctgacga cgccctggac
     1321 cggctgctcg aggagaccgc gctcgtcgcc gaccaggatg cgtacgaggg cgacgccgac
     1381 gccgtcacgc tgatcacgct ccacgcggcg aagggcctcg agttcccggt ggtgttcatc
     1441 gcgggcctcg aagaggggct gttcccgcac aaccgcgcgc tggacgacga gaaggagctg
     1501 gaagaggagc gccggctcgc gtacgtcggg atcacgcggg cgaagcggcg gctgtacctg
     1561 tcgcacgcgt ggcgccgggc gacctggggc ggcggcggca tgagcgtccc gtcgcgcttc
     1621 ctcctcgaga tcccggccga gctgatggtc gggccgcagc tccagcgcgg ggccgactac
     1681 ggggtcgaca tcgacccgga cctcgtgttc ggggaccggc ggtcgcggtt cgggcaggcg
     1741 atccggtcgg gcggcggcgc ctaccgggag gggagcggcc gtccgggggc gccggcggcc
     1801 ggggaggcgt tccgcccctc aagggacctc ggggcgaagc gcgaggcgtt cgccgcgggc
     1861 gccccgtcgg ggtctctggg gccgcagccg cggtccaccc cgagcgtccc tgcacgaccc
     1921 atcgtccccg gcgagcgc
//
LOCUS       DBNHXO010000347         2053 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4066668, whole genome shotgun sequence.
ACCESSION   DBNHXO010000347 DBNHXO010000000
VERSION     DBNHXO010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4066668"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="AC162A_07215"
     CDS             <1..1341
                     /locus_tag="AC162A_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5010668.1"
                     /translation="QDAIRAARRAQPAWFRLGWEKRLEIMKRAADLISERQMEYAGLM
                     AVEIGKTRIEALGEVEEAADLIRYYAKTAEDNAFYDHPLDNLGDATVHTRTILRPHGI
                     FAVISPFNFPMALSVGPTCAAMMAGNTTILKPASAGAMTAIAVLEAYRDAGVPDGVFN
                     LVMGPGDTVGDELQHNQGIDGIVFTGSYEVGFELFRNFSTTFPRPCIVEMGGKNPAIV
                     LRSADLEEAAEGIMRAAFGFGGQKCSANSRVYVEKPVHDELVRLLVEKTEKLTVGDPL
                     PRTAFLGPVIDQRAVDRHQRAVADARRDGAVFTGGEHLTDGALARGFYVEPTVVGNLP
                     RSHRLFQDELFAPFTAVHAVDSLDEALALANDNVYGLTAGVYSSEPAEVQRFLDEIEA
                     GVLYVNRRAGATTGAWPGVQAFGGWKGSGSTGKAGLSMYYVAQFLREQSHTVVD"
     gene            1668..>2053
                     /locus_tag="AC162A_07220"
     CDS             1668..>2053
                     /locus_tag="AC162A_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5010669.1"
                     /translation="MTTPLEARTVPDIVTELPGPRARAHIEFDHAWTSPSLPRAYPIV
                     PVRGLGCAIEDIDGNVFLDFAAGIAVNSTGHSHPDVVAAIQRQAAELQHFSASDFYLP
                     IYAEAAAELARIAPMSPARVFIGNSGA"
BASE COUNT          320 a          734 c          709 g          290 t
ORIGIN      
        1 caggacgcga tcagggccgc ccgcagggcg cagccggcct ggttccggct cgggtgggag
       61 aagcgactcg agatcatgaa gcgcgcggcg gacctgatct ccgagcggca gatggagtac
      121 gcgggcctga tggccgtcga gatcggcaag acgcggatcg aggcgctggg cgaggtcgag
      181 gaggccgccg acctcatccg ctactacgcg aagacggccg aggacaacgc gttctacgac
      241 cacccgctcg acaacctcgg cgacgcgacc gtccacaccc ggacgatcct gcgaccgcac
      301 gggatcttcg cggtcatcag cccattcaac ttcccgatgg cgctgtccgt cgggccgacg
      361 tgcgcggcga tgatggccgg caacaccacg atcctcaagc cggcctcggc cggcgcgatg
      421 accgcgatcg cggtgctcga ggcgtaccgc gacgccgggg tcccggacgg cgtgttcaac
      481 ctggtcatgg ggccgggcga caccgtcggc gacgagctcc agcacaacca gggcatcgac
      541 gggatcgtgt tcacgggctc gtacgaggtc gggttcgagc tgttccgcaa cttctccacg
      601 acgttcccgc ggccgtgcat cgtggagatg gggggcaaga acccggcgat cgtgctgcgc
      661 tcggcggacc tcgaggaggc ggccgagggg atcatgcggg cggccttcgg gttcggcggg
      721 cagaagtgct cggccaacag ccgggtctac gtcgagaagc ccgtccacga cgagctggtc
      781 cggctgctcg tcgagaagac cgagaagctc acggtcggcg acccgctgcc gcggaccgcg
      841 ttcctggggc cggtcatcga ccagcgggcg gtcgaccgtc accagcgtgc cgtggccgac
      901 gcccggcgcg acggcgccgt gttcaccggc ggcgagcacc tgaccgacgg tgcgctcgcc
      961 cgcggcttct acgtcgagcc cacggtcgtc ggcaacctcc cgagatcgca tcgcctcttc
     1021 caggacgagc tgttcgcgcc gttcaccgcg gtccacgcgg tggactcgct cgacgaggcg
     1081 ctcgcgctgg cgaacgacaa cgtctacggg ctgaccgcgg gcgtctactc gtccgagccg
     1141 gcggaggtgc agcgcttcct cgacgagatc gaggcaggcg tgctgtacgt caaccggcgc
     1201 gccggagcga cgacgggcgc ctggcccggc gtccaggcgt tcggcggctg gaagggctcc
     1261 ggatcgaccg gcaaggcagg cctgtcgatg tactacgtcg cgcagttcct gcgggagcag
     1321 agccacaccg tcgtcgacta gcgccggagc acctcgccgc cggcccgtgt gcctgagacc
     1381 cgccaaatcg ctccatcggc gccatcggag gcaggccagc ggcgagaaac gtgcctgaca
     1441 gtcacgcgat cgagcgcaac ggccgattca gctgtctgga tggcgcgttt tggccgatcc
     1501 tgggcacatc tcgctggtcg ggtcgcggga ccttcgggtc gtcgcgacgg ggcgccgcct
     1561 cctgctattc tgatattcac tctgcataac atctatgcgt atctcggcgc ccgccgaacg
     1621 gcggcggggc ctcgaccgac gctcggatcc acagtcaagg aggccacgtg acgaccccgc
     1681 tcgaggcgcg aaccgtcccg gacatcgtca ccgagctgcc cggccccagg gccagggcgc
     1741 acatcgagtt cgaccacgcc tggacgagcc cgagcctccc gcgggcctac ccgatcgtgc
     1801 ccgtgcgcgg cctcggctgc gcgatcgagg acatcgacgg caacgtgttc ctcgacttcg
     1861 ccgcgggcat cgccgtcaac tcgacgggcc acagccaccc cgacgtcgtc gccgcgatcc
     1921 agcgccaggc ggcggagctc cagcacttca gcgccagcga cttctacctg ccgatctacg
     1981 ccgaggccgc cgccgagctc gcccgcatcg cgccgatgtc gccggcccgc gtgttcatcg
     2041 gcaactccgg cgc
//
LOCUS       DBNHXO010000348         3434 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3075093, whole genome shotgun sequence.
ACCESSION   DBNHXO010000348 DBNHXO010000000
VERSION     DBNHXO010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3075093"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /locus_tag="AC162A_07225"
     CDS             complement(<1..1354)
                     /locus_tag="AC162A_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COG1470 family protein"
                     /protein_id="HIR5010670.1"
                     /translation="MSAVTTLSRADPTVDPGGQAAITITIRNSGAIVDRFDVDVVGPT
                     AGWARVDPPSLSLFPGVEGSVTITFSPPRASTPRAGLHPFGIRVRPAADPSGSAVEEG
                     RISVTPFTAVAADVVPQTSRGSKVGRHQVVVVNRGNAPSDVTVNAIDPDRRLKLDVQP
                     PRTVVAPDERSEFGVRVEVDDPFPFGAARQRQFQISVEPGRQQPIQLRPALSQLPMLP
                     GWIPPVAAVVAIVAVLGIGAFLAKAGPFTPEATAVPSQLAEATTAPPPSAPPSVEPPS
                     EEPPSEAPVSEGPPTPSPTPPPLKPGDFTLTVTGDDVALGGDLSLLCQASDKACRRAV
                     KEQVLAMVNELQNPYTGQGIQSTGSTDQPNTLPLVMTAERDVPWSQAGGGQAGVTQKI
                     VLDLGPLLAVPPTPPYAVVNTPDGIPHRFVVDAGNAKGLFDRLYQLPPAMSIAPATPP
                     P"
     gene            1454..2887
                     /locus_tag="AC162A_07230"
     CDS             1454..2887
                     /locus_tag="AC162A_07230"
                     /inference="COORDINATES: protein motif:HMM:NF024298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HIR5010671.1"
                     /translation="MIVCKVCGATNEQGATFCGTCGAFLEWSGEQVQDGSTQGGVAPP
                     IYPPPPDVAAPTTGPASDPVPVPPNPEPAPPGSIVCPNCGQINESTRVYCSRCATELA
                     AVAIATDQVVAPPPSSRSIPPVAILGAVGGVVLLAVLAFAVFGPKSTPPPSGAVTEPP
                     GTTRPSLQPSDAVTSAPPSDAVESTPPSNEPESQPPPEVTGTLAFGAEKGDNADIYVW
                     AAGDEKPHKLFGGNGDETDPAWSPDGSKVAFALNVGNFRPNGGTPDEGIRVIKADGTE
                     AKVFDFTHHDVDRNPAWSPDGETIVFASTRDHADNKNLDLYSRVYEATSDEEALADDD
                     ADDWDPAWSPDGETIIFVSKRDGPAQLFTMTAEGKRQEALDLGDGVFDDPTYSRVDGL
                     LAYTRREANGAQKQLFVANDDGSDARQVGSFTGDVSDPTWSPDAKLLAVANGDTGEID
                     IVDVATGTVVESLSVPNATLMQPDWTE"
     gene            3094..>3434
                     /locus_tag="AC162A_07235"
     CDS             3094..>3434
                     /locus_tag="AC162A_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HIR5010672.1"
                     /translation="MPSPDALPRERLEARLADAWRHRLTLVVAPAGSGKTTLVARFAA
                     QATVPVGWYRAETWDADEASFVRHLEAALRSAMPDLPGGWTTVDDVAKALESNPAPAA
                     TLVIDDGHALEG"
BASE COUNT          494 a         1222 c         1259 g          459 t
ORIGIN      
        1 cgggcggtgg cgttgccggg gcgatcgaca tggccggcgg gagctggtag agccggtcga
       61 acaggccctt cgcgttcccc gcgtcgacga cgaaccggtg ggggatccca tcgggcgtgt
      121 tgacgaccgc gtacgggggg gtcggcggaa cggcaagcag cggaccgagg tcgagcacga
      181 tcttctgggt cacgccggcc tggccgccgc cggcctggct ccacgggacg tcccgctcgg
      241 cggtcatcac cagggggagc gtgttgggct gatcggtcga gcccgtgctc tggattccct
      301 ggcccgtgta cgggttctgg agctcgttga ccatcgcgag gacctgttcc ttgacggccc
      361 ttcggcaggc cttgtcggac gcctggcaga ggaggctcag gtccccgccc agcgcgacgt
      421 cgtcgccggt caccgtcagc gtgaagtcgc cgggcttgag cggcggcggc gtcggcgatg
      481 gcgtcggcgg cccttccgag accggcgcct ccgacggggg ctcctcgctg ggtggctcca
      541 ccgacggtgg ggcggaaggc ggcggcgcgg tcgtggcctc ggccagctgg ctcgggaccg
      601 cggtggcctc cggggtgaac ggtcccgcct tggccaggaa cgccccgatg cccaggacgg
      661 cgacgattgc gacgaccgcg gccaccggcg ggatccagcc ggggagcatc ggcagctggc
      721 tgagggccgg tcgcagctgg atcggctgct ggcggccggg ttcgacgctg atctggaact
      781 ggcgctggcg ggcggcgccg aacgggaacg ggtcgtcgac ctccacccgg acgccgaact
      841 cgctccgctc gtcgggcgcg acgaccgtgc gcggtggctg gacgtcgagc ttgagccgcc
      901 ggtcggggtc gatggcgttg acggtgacgt cggacggcgc gttgccgcgg ttcacgacga
      961 cgacctggtg gcgtccgacc ttggagccac gggacgtctg cgggacgacg tcggccgcca
     1021 cggcggtgaa cggggtgacc gagatccgcc cctcctcgac ggctgacccc gacggatcgg
     1081 cggccggccg gacgcggatg ccgaacgggt ggagcccggc ccgcggcgtg cttgcccggg
     1141 ggggcgaaaa cgtgatcgtg accgagccct cgacgcccgg gaacagcgac aggctcggcg
     1201 ggtcgacgcg ggcccagccg gcggtcggcc cgacgacgtc gacatcgaac cggtcgacga
     1261 tcgcccccga gttccggatc gtgatcgtga tcgcggcctg gccgccggga tcgaccgtcg
     1321 ggtcggcacg cgagagagtg gtcacggcgc tcatgtcccg cagtctacgc ggagggcgcg
     1381 gagacgcggg cgcgcccggc cgggcccccg ccggttagac tggccccccg gatcgaagtg
     1441 cggagaagcc ggcgtgatcg tctgcaaggt ctgcggcgcg acgaatgagc agggggcgac
     1501 cttctgcggc acgtgcggcg cgttcctcga gtggagcggc gagcaggttc aggacggctc
     1561 cacgcaggga ggtgtcgcgc ctccgatcta tccgccgccg cctgacgtcg ccgcgccgac
     1621 cacggggccg gcctccgacc ccgttccagt cccgccgaac ccggagcctg ccccgccggg
     1681 ctcgatcgtc tgccccaact gcggccagat caacgagtca acccgcgtgt actgctcgcg
     1741 ctgcgccacc gagctcgcgg cggttgctat cgcgaccgac caggtcgtcg cgccgccgcc
     1801 cagctcgcgc agcatcccgc ccgtcgcgat cctcggagcc gtcggcggtg tcgtcctgct
     1861 ggccgtgctc gcgttcgcag tgttcgggcc caagagcacg ccgcccccgt cgggcgccgt
     1921 caccgagccg cccggcacga cccgacccag cctccagccg tccgatgccg tcacctccgc
     1981 gcctccctcg gatgccgtcg aatcgacccc gcccagcaac gagcccgagt cccagccacc
     2041 gccggaggtc accggcaccc tcgcgttcgg cgcggagaag ggcgacaacg ccgacatcta
     2101 cgtctgggcg gcgggcgacg agaagcccca caagctgttc ggcggcaatg gggacgagac
     2161 cgaccccgcc tggtcgcccg atggctcgaa ggtcgcattc gcgctcaacg tcggcaactt
     2221 ccgccccaac ggcggaacgc cggacgaggg catacgggtg atcaaggccg acgggacgga
     2281 ggcgaaggtg ttcgacttca cccatcacga cgtcgaccgc aatcctgcct ggtcgcccga
     2341 cggcgagacg atcgtgttcg cgtccacccg cgatcacgcc gacaacaaga acctcgacct
     2401 gtactcgcgg gtgtacgagg cgacgtccga cgaggaggct ctcgccgacg atgacgcgga
     2461 cgactgggat cctgcctggt cgcccgacgg cgagacgatc atcttcgtct cgaagcgcga
     2521 cgggccggcg cagctgttca ccatgaccgc ggaggggaag aggcaggagg cgctcgacct
     2581 cggcgatggt gtcttcgatg acccgaccta ctcgcgcgtc gacggcctgc ttgcctacac
     2641 tcgccgcgag gcgaacggcg cgcagaagca actcttcgtc gccaacgatg acggttcgga
     2701 cgcgcgccag gtcggatcgt tcaccggcga cgtcagcgac ccgacgtggt cgcccgacgc
     2761 gaagctgctc gccgtcgcca acggggatac cggcgagatc gacatcgtcg acgtcgcaac
     2821 gggcacggtc gtcgagtcgc tcagcgtccc gaatgcgacg ctgatgcagc cggattggac
     2881 cgaatagaga ggaccgcagt aacgggacca tcggatcccg cccgggacgg gatcaagggt
     2941 ggacccaggc accccccggg tgcaccatag gcaggccccg ccgacgggga gtgcgaggct
     3001 ggagggcggc gattgcgaga ccggcaacgt ccggctcggg aagggcagcg gccgcgtgcc
     3061 gccgtgcccc tcgtggtacc cgcgaagatc cgggtgccgt ctccggacgc cctgccccgt
     3121 gagcgcctgg aggcccgcct cgccgacgcc tggcgccatc ggctgacgct ggtcgtcgcg
     3181 cccgcgggct ccggcaagac gacgctcgtc gcccggttcg cggcccaggc gacggttccc
     3241 gtcggctggt atcgagccga gacctgggac gccgacgagg cctcgttcgt ccgccacctc
     3301 gaggcggcgc ttcggtccgc catgccggat ctccccggtg gctggaccac ggtcgacgac
     3361 gtcgccaagg ccctggagtc gaacccggcc cccgcggcca cgctcgtgat cgacgacgga
     3421 cacgcgctcg aggg
//
LOCUS       DBNHXO010000349         3775 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_99371, whole genome shotgun sequence.
ACCESSION   DBNHXO010000349 DBNHXO010000000
VERSION     DBNHXO010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_99371"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1526
                     /locus_tag="AC162A_07240"
     CDS             <1..1526
                     /locus_tag="AC162A_07240"
                     /inference="COORDINATES: protein motif:HMM:NF015706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR5010673.1"
                     /translation="GVVPMGPPLVTSEEQHRTLLAEILAEHDEAILRSFVSDGAISYR
                     RLREALATQTRRGWVHPVYVGSAGRGEGIDALLAGIDELLSGERGDRDAPVSGRVFKI
                     ERTPAGERVAYVRLFAGTVRPRQRVRVGGGEEAKPTSIKVFAPAGAPRRDVLAAGEMA
                     AIRGLGAVRVGDAIGVPLPGQAETARFPRPALEAVVFPLRSEQAGSLRAALAQLAEQD
                     PLIDVRQDDHRHEIAVSLYGEVQKEVIGATLERDYGIAADFRETTVVCIERPARAGDA
                     EEIIRAKTHTNITGRSSPLSTNPFLATLALRVEPLAPGAGIAFRADVEHRLVPLYLFK
                     TPETFVTQMEAYVHEALTEGLAGWQVTDCLVTMTDCGYSSPATSAADFRRLTQLVLMT
                     ALDRAGTWVCEPLADLALEMPTSTAPGVLGALGRLGGRVTGQFSAGGLSKASAVLPVA
                     RLRSLQQQLPGLSMGEGILETRPDGYQPIGSDPPRRERSAPSPLDRDAWLASLAKRG"
     gene            1588..2919
                     /locus_tag="AC162A_07245"
     CDS             1588..2919
                     /locus_tag="AC162A_07245"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5010674.1"
                     /translation="MIPETRYARLGELHLAYQVLGDGPPDILLLDQWFGNLDAQWDVP
                     PLAALRERLSTFGRLIMFDKRGTGLSDPIPTSTLPTLEEFMTDIPAVLDTVGCERAAL
                     IANIGGGILAIPFAAAHPDRVSSLILVDCFARFLEAPDFPIGAPVAELAPALEIAEDE
                     TGRGVMIDLFAPSVAGDERIRRAWARYERGAATPGSTKAIVRLIYESDVRDVLPAIRV
                     PTLVIHRAAASGFRVEHGRYLAEHIPGAKYVELPGPDNLIWAGDQDAVVAEIQDFVTG
                     VRPVPDPRRVLATVLFTDIVGSTQLAAERGDSAWQRLLADHNRIVRRQLGRFGGVEIK
                     VIGDGFLATFDGPARAIRCAAAIGDDVSELGLEIRAGLHVGEIEVLDNDIAGLAVHIA
                     ARVSAKAGAGEILVSSTVKDLVVGSGLEFDDRGTHDLKGVPGAWHLFAVRG"
     gene            3016..>3775
                     /locus_tag="AC162A_07250"
     CDS             3016..>3775
                     /locus_tag="AC162A_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016880787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5010675.1"
                     /translation="MDENAWLAERFEANRPHLRGVAYRMLGSVTDADDAVQEAWIRLS
                     RTDTSEVENLRAWLTTVVGRVCLNMLRARGTRREASLETHVPDPIVGPEQGNDPEGEA
                     LLGDAVGLALFVVLDSLSPAERVSFVLHDVFAVPFDDIAPIVGRTPTATRQLASRARR
                     RVQGAPVPDVDLTSQWAIVDAFLAAARDGDFERLLAVLDPDVVLRADAGEERPTLTAM
                     VRGAAAVAGQALTFRNCAATATRVLVNGIPGGVAW"
BASE COUNT          510 a         1367 c         1356 g          542 t
ORIGIN      
        1 cgggcgtcgt gccgatgggc ccgccgctgg tcacgtccga ggagcagcac cggacgctgc
       61 tcgcggagat cctcgcggag cacgacgagg cgatcctccg gtcgttcgtg agcgacggcg
      121 ccatctcgta ccggcgcctt cgcgaggccc tcgccacaca gacgcgacga ggctgggtgc
      181 acccggtgta cgtcgggtcg gcggggcgag gggaggggat cgacgcgctg ctggccggca
      241 tcgacgagct cctatccggt gagcgcggcg atcgcgatgc gccggtctcc ggccgcgtgt
      301 tcaagatcga gcggacgccg gcgggcgagc gcgtcgccta cgtccgcctg ttcgcgggca
      361 ccgtgcggcc gcgccagcgg gtgcgggtcg gcgggggcga ggaggcgaag ccgacatcga
      421 tcaaggtgtt cgcgccggcc ggagcgccgc ggcgtgacgt cctggccgcg ggtgagatgg
      481 ccgcgatccg ggggctcggt gccgtccggg tcggtgacgc gatcggtgtg ccgcttcccg
      541 gtcaggcgga gaccgcgcgc ttcccgcgtc cggcgctcga ggccgtcgtg ttcccgctcc
      601 gatcggaaca ggccggcagc ctgcgggcgg ccctcgcgca gctcgccgag caggacccgc
      661 tcatcgacgt ccgccaggac gaccaccgcc acgagatcgc ggtctcgctc tacggcgagg
      721 ttcagaagga ggtcatcggc gcgacgctag agcgcgacta cggcatcgcc gccgacttcc
      781 gcgagacgac cgtcgtgtgc atcgagcgcc cggcccgggc gggggacgcc gaggagatca
      841 tccgggcgaa gacgcatacc aacatcaccg gccggagctc gccgctgagc acgaacccgt
      901 ttctggcgac cctggcgctg cgggtcgagc ctctcgcgcc gggcgctggc atcgcgttcc
      961 gcgccgacgt cgagcaccgc ctcgtcccct tgtacctgtt caagaccccg gagacgttcg
     1021 tcacccagat ggaggcgtac gtccacgagg cactcaccga ggggctggca ggctggcagg
     1081 tgaccgactg cctggtcacg atgaccgact gcggctactc gtcgccggcg acgtccgcgg
     1141 cggacttccg gcgcctgacg cagctcgtgc tgatgacggc gctcgaccga gccggcacgt
     1201 gggtctgcga gccgctcgcc gatctcgcgc tcgagatgcc gacgtcgacc gcccccggtg
     1261 tgcttggcgc actcggccgg ctcggcgggc gcgtgaccgg ccagttctcg gcgggcggcc
     1321 tgtccaaggc gagtgcggtc ctgcccgtcg cgcgcctccg gtcgctgcag cagcagctcc
     1381 ccggcctgtc gatgggggag gggatcctcg agacccgccc cgacggctac cagccgatcg
     1441 gcagcgaccc gccccgccgc gagcgctcgg cgccgagccc cctcgaccgc gacgcctggc
     1501 tcgcgtccct cgccaagcgc gggtagctcg gccgacgacg ccgtagtatc cgtgcgtgaa
     1561 cgggaccgtg ggcatcgggg ccgacgcatg atccccgaga cgcggtacgc gcggctcggc
     1621 gagttgcacc tcgcctacca ggtcctcggc gacgggccgc ccgacatcct gctactcgac
     1681 cagtggttcg gcaacctcga cgcccagtgg gacgtgccgc ccctggcagc gctccgggaa
     1741 cggctgtcga cgttcggtcg gctgatcatg ttcgacaagc gggggaccgg gctatcggat
     1801 ccgatcccga cgtcgacgct gccgaccctc gaggagttca tgacggacat cccggccgtg
     1861 ctcgacacgg tcggctgcga gcgagcggcg ctgatcgcga acatcggcgg cggcatcctc
     1921 gcgatacctt tcgccgccgc gcatcccgac cgggtctcga gcctcatcct cgtcgactgc
     1981 ttcgcccggt tcctcgaggc gcccgacttc ccgatcggag cgcccgtcgc cgagctggcc
     2041 ccggcgctgg aaattgcaga ggacgaaacc ggacggggag tgatgatcga cctgttcgcg
     2101 ccgtccgtcg cgggcgatga gcgcatccgc cgcgcgtggg cccggtatga gcgtggggcg
     2161 gcgacgcccg gcagcaccaa ggcgatcgtc cgcctgatct acgagagcga cgtccgggac
     2221 gtcctgcctg cgatccgcgt cccgacgctc gtcatccacc gggcggccgc ctcggggttc
     2281 cgagtcgaac acgggcgcta tctcgccgaa cacatcccgg gtgcgaagta cgtcgagctc
     2341 ccgggtcccg acaacctcat ctgggccggc gatcaggacg cggtcgtcgc cgagatccag
     2401 gacttcgtca ccggcgtccg cccggtaccc gatccccgcc gcgtgctggc gaccgtgctg
     2461 ttcaccgaca tcgtcggctc gacccagctg gcggccgaac gcggcgacag cgcatggcaa
     2521 cggctgttag cggaccacaa ccggatcgtc cggcgccagc tcggccgctt cggcggcgtg
     2581 gagatcaagg tcatcggaga cggcttcctg gccacattcg acggcccggc acgggcgatc
     2641 cggtgtgccg ccgcgatcgg cgacgacgtc agcgagctcg ggctcgagat ccgggccggg
     2701 ctccacgtcg gcgagatcga ggtcctcgac aacgacatcg ccgggctcgc ggtgcacatc
     2761 gcggccaggg tgtccgcgaa ggccggcgcc ggcgagatcc tcgtctcgag cacggtcaag
     2821 gacctcgtcg tggggtccgg tctcgagttc gacgatcgtg gcacgcacga cctgaagggc
     2881 gtcccgggcg cctggcacct gtttgctgtt cgcggttagc atcgggctac gggggacctg
     2941 cccgggtcac gctgtcacgt ctggcccgcc ggcggggtca cgtccatgac accaatggac
     3001 gcgaggacct gatcagtgga cgagaacgcc tggctggcgg agcgattcga agccaaccga
     3061 ccgcacctcc ggggcgtggc ctaccggatg ctcggctccg tgaccgatgc ggacgatgcc
     3121 gtccaggagg cgtggatccg cctcagccgc actgacacga gcgaggtcga gaacctgcgc
     3181 gcgtggctga cgacggtcgt cgggcgcgtc tgcctcaaca tgctgcgggc gcgcgggacg
     3241 cggcgcgagg cgtcgctcga gacccacgtc ccggatccca tcgttggccc ggagcagggg
     3301 aacgatcccg agggggaggc cctcctgggt gacgccgtcg ggctcgcgct gttcgtggtc
     3361 ctcgactccc tctcgcccgc cgagcgcgtc tcgttcgtcc tccacgacgt gtttgccgtg
     3421 ccgttcgacg acatcgcgcc gatcgtcggc cgaaccccta cggcgacccg ccagctggcg
     3481 agccgggccc gccgccgcgt gcagggtgcg ccggtgcccg acgtcgacct caccagccag
     3541 tgggccatcg tcgacgcgtt ccttgcggcc gcgcgcgacg gcgacttcga acgcctgctc
     3601 gcggtcctcg acccggacgt cgtgctccgc gccgacgccg gcgaggagcg cccgactctg
     3661 acggccatgg tccgcggcgc ggcggccgtg gcgggccagg cgctgacgtt ccgcaactgc
     3721 gccgcgacgg cgacacgcgt gctcgtcaat ggcatccccg gcggtgtcgc ctggg
//
LOCUS       DBNHXO010000350         2282 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3075197, whole genome shotgun sequence.
ACCESSION   DBNHXO010000350 DBNHXO010000000
VERSION     DBNHXO010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3075197"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="AC162A_07255"
     CDS             complement(<1..580)
                     /locus_tag="AC162A_07255"
                     /inference="COORDINATES: protein motif:HMM:NF021287.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HIR5010676.1"
                     /translation="MSLYRLAEPMPSIDQPIVLAAFDGWVDAGTAATSILSILAEDGE
                     TVATFDPDQLFDYRSRRPTLEIRDGRLSSLVWPEVTLRLARYSGLDLLVLTGAEPDDR
                     WHLLAESVVTLLGELGTRGWISLGAIPAAVPHTRPVPILGTESEAGLLRGGVTAGPMG
                     ILRVPSAALSMLEMAVSDAGIPAVGYFAQIPHY"
     gene            682..1176
                     /locus_tag="AC162A_07260"
     CDS             682..1176
                     /locus_tag="AC162A_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010677.1"
                     /translation="MGSVMKGLSALLFAWFVFMIGAAIYAAIKRREVELPDPAADEVD
                     LVASFGPLEFHSEAAAFRGGSVTTMFGGGELDLRDATLDPAGATLQIKTLFGGGNLVV
                     PEAWNIETKLSGIGGVGDGRAKVERSPDAPTLRLEGTNWFGGWGITSTPANEDDVLPE
                     PVTV"
     gene            1269..>2282
                     /locus_tag="AC162A_07265"
     CDS             1269..>2282
                     /locus_tag="AC162A_07265"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HIR5010678.1"
                     /translation="MIRTTPFHARTAELNESGLWSHWSGHLAAEKYGLSEKFEYFAVR
                     NAAGLFDTSPLFKYRFSGPGAERFLAGVMARDPRVLEPGHGMYAIWCDDRGFVVEDGV
                     LLRHKKDEFVLTAAEPNLAYFQDLVTRRDKVTIEEVSQDWGVLSLQGPRARRILAGLV
                     PSVESLPYFGITSGKIGGCPVTISRTGYTGDLGYEVWVEAENALPTWDALWESMRGFG
                     VLPFGLQALYMTRIEAGLLLLDVDFASSRFAWTDADRATPQELGLSWMLKGVDTDDRA
                     FIGRDAIRREAAEKTSRFRTTGIVVDWRDWDRIHDEAGLIPPKDHSPVQEEMFLYDAD
                     GDQV"
BASE COUNT          354 a          762 c          832 g          334 t
ORIGIN      
        1 cgtagtgcgg gatctgcgcg aagtagccga ccgccgggat tcccgcgtcg gaaacggcca
       61 tctcgagcat cgagagcgcc gccgacggca cgcgcaggat gcccatcggg ccggccgtga
      121 cgccgccccg cagcagccca gcctcggact ccgtgccaag aatggggacc gggcgcgtgt
      181 gcgggacggc cgccggaatc gccccgaggc tgatccatcc ccgcgtgccg agctccccga
      241 gcagcgtcac caccgactcg gcgaggaggt gccagcgatc gtcgggctcg gcacccgtga
      301 ggaccagcag gtcgaggccg ctgtagcggg ccagccggag cgtgacctcc ggccagacca
      361 aggacgacag gcgcccgtcg cggatctcga gggtcggccg ccgcgagcgg tagtcgaaca
      421 gctggtcggg gtcgaacgtg gcgaccgtct cgccgtcctc cgcgaggatc gagaggatgc
      481 tggtggccgc cgtccccgcg tcgacccagc cgtcgaacgc cgcgagcacg atcggttgat
      541 cgatcgacgg catcggctcg gcgaggcgat agagcgacat gcgcgaagga tacgggaggc
      601 cgttcggccc tcgaccatgc gcggcatcgg gtgcatcctt ggcgggccgg ggaaagtccg
      661 tatctcgatg ggagtttcaa catgggaagc gtcatgaagg gcctcagcgc cctgctgttc
      721 gcctggttcg tgttcatgat cggggccgcg atctacgccg ccatcaagcg gcgcgaggtc
      781 gagttgccgg atcccgcggc cgacgaggtc gacctcgttg ccagcttcgg gccgctcgag
      841 ttccacagcg aggctgcggc cttccgcggc ggctcggtga cgaccatgtt cggcggtggc
      901 gagctcgacc tgcgtgacgc gacgctcgac ccggccggag cgaccctcca gatcaagacc
      961 ctgttcggcg gcggcaacct ggtggtgccg gaggcctgga acatcgagac caagctgtcc
     1021 gggatcggtg gcgtcgggga cggccgggcc aaggtcgagc gatcgcccga cgcgcccacg
     1081 ctccgcctcg agggcacgaa ctggttcggc ggctggggaa tcacctcgac gccggccaac
     1141 gaggacgacg tcctgccgga gcccgtcacc gtctgaaccg gagctgatgc gcggcgggca
     1201 acgcgccgcc acggttggtc gcccgtcggg taccgtcggc ggtccaaccg ggaggcaagc
     1261 acgtcgcaat gatccggacg accccgtttc acgcccgcac ggccgagctc aacgagtccg
     1321 gcctgtggag tcactggtct gggcacctgg ccgccgagaa gtacggcctg tccgagaagt
     1381 tcgagtactt cgcggtccgt aacgcggccg ggctgttcga cacctcgccg ctgttcaagt
     1441 accggttctc cggtccgggt gccgagcgct tcctcgccgg cgtcatggcg cgcgacccgc
     1501 gcgtgctcga gccggggcac gggatgtacg cgatctggtg cgacgaccgg gggttcgtgg
     1561 tcgaggacgg cgtcctgttg cggcacaaga aggacgagtt cgtcctcacg gcagcggagc
     1621 ccaacctcgc ctacttccag gacctcgtga cccggcgcga caaggtcacg atcgaggagg
     1681 tctcgcagga ttggggcgtg ctcagcctcc aggggccgcg cgcgcgccgg atcctggccg
     1741 ggctcgtgcc atcggtcgag tcgttgccgt acttcgggat cacgagcggc aagattggcg
     1801 gctgtccggt gaccatctcg cgcaccggct acaccggcga cctgggctac gaggtctggg
     1861 tcgaggccga gaatgccctg ccgacgtggg acgcgctgtg ggagtcgatg cgcggcttcg
     1921 gggtgctgcc gttcggactc caggcgctgt acatgacccg catcgaggca gggttgctgc
     1981 tgctcgacgt cgacttcgcg tcgagccggt tcgcgtggac ggacgccgac cgggcgacgc
     2041 cccaggagct cggcctcagc tggatgctca agggcgtgga caccgacgat cgggcgttca
     2101 tcgggcgcga cgcgatccgc cgcgaggccg ccgagaagac ctcacgcttc cggacgacgg
     2161 ggatcgtcgt cgactggcga gactgggacc gcatccacga cgaggccggg ctgatcccgc
     2221 ccaaggacca cagtccggtg caggaggaga tgttcctgta cgacgcggac ggtgaccagg
     2281 tg
//
LOCUS       DBNHXO010000351         1601 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1489669, whole genome shotgun sequence.
ACCESSION   DBNHXO010000351 DBNHXO010000000
VERSION     DBNHXO010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1489669"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="AC162A_07270"
     CDS             complement(<1..455)
                     /locus_tag="AC162A_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HIR5010679.1"
                     /translation="MSAPGVADARDAVALVFREDHGRALATLIRVLGDFDLAEEAVAD
                     AYLVALEHWPVDGIPDNPGAWITTTARNRAIDRIRRARRFADKAEVIARDSAFDADHA
                     AAALLLAAEDEMHPIPDDQLRLIFTCCHPALAPESAVALTLRTLGGLTTP"
     gene            complement(452..799)
                     /locus_tag="AC162A_07275"
     CDS             complement(452..799)
                     /locus_tag="AC162A_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR5010680.1"
                     /translation="MQYLLLVYLEPSAQPTDDAAIREMFDEYRVFTRSLQESGMFLGG
                     EALQDVANATSVTVRDGRRLVTDGPFAETKEHLAGFYLIEAPDLDAAIDAAARVPDAK
                     IGTVEVRPILDMA"
     gene            970..>1601
                     /locus_tag="AC162A_07280"
     CDS             970..>1601
                     /locus_tag="AC162A_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HIR5010681.1"
                     /translation="MASRKEILEIGGREVAVSNPDKVYFPVPGYTKLDLVHYFLAVAD
                     GALAGVRGRPMALKRFVDGIDKEPFFQKRAPDNRPEWMRVATLTFPSGRTADEIVVDE
                     AAGLVWVANLGCIDLNPHPVRAEDMEHPDELRVDLDPVPGVPWSQVREVALVVQESLE
                     AVGLVGWPKTSGSRGIHVNVRIESRWTYSQVRRAALALARDVERRAPSIAT"
BASE COUNT          219 a          564 c          582 g          236 t
ORIGIN      
        1 ggcgtcgtca ggccgccgag cgtgcgcagg gtcagcgcga ccgccgactc cggcgccagc
       61 gccgggtggc agcaggtgaa gatcagtcgc agttggtcgt cggggatcgg gtgcatctcg
      121 tcctccgccg ccaagagcag cgccgcggcc gcgtggtccg cgtcgaacgc gctgtcgcgc
      181 gcgatgacct cggccttgtc cgcgaagcgg cgcgctcgcc ggatgcgatc gatggcacgg
      241 ttgcgtgccg tcgtcgtgat ccacgccccc gggttgtcgg ggatcccgtc gaccggccag
      301 tgctcgagcg ccaccaggta ggcatcggcg accgcctcct ccgcgaggtc gaagtccccc
      361 aggacccgga tgagggtcgc gagcgcccgg ccgtgatcct cgcgaaagac gagggcgacg
      421 gcatcgcggg cgtcggctac cccgggcgcg ctcaggccat gtccaggatc ggccgcacct
      481 cgacggtccc gatcttcgcg tcggggacac gcgctgcagc gtcgatcgcc gcatcgaggt
      541 ccggcgcctc gatcaggtag aacccggcca ggtgctcctt ggtctcggcg aacggcccgt
      601 cggtcacgag ccggcgcccg tcgcgcacgg tcacggacgt ggcgttggcg acgtcctgga
      661 gcgcctcgcc accgaggaac atgcccgatt cctggagcga gcgggtgaag acgcggtact
      721 cgtcgaacat ctcgcggatc gccgcgtcgt cggtcggctg cgccgacggc tccaggtaga
      781 ccagcaacag gtattgcatc ggcgtctcct cgtccgggtg ccggccggtc cgtcccgacc
      841 cgtgcatcct agcgacgatt cggaccgccg cgtatcgaca tccgtcaggc ggtcacgggt
      901 tctgtcgaca accggtgcgc ccgccgcaga gaactggatc ggcgcgtcgt cccgtgcgag
      961 catgcgggca tggcgtcgcg caaggagatc ctcgagatcg gcggacggga ggtcgcggtc
     1021 tcgaacccgg acaaggtcta cttcccggtt ccgggctaca cgaagctcga cctcgtccac
     1081 tactttctcg ccgtcgccga cggcgcgctg gccggggtcc ggggccggcc gatggccctc
     1141 aagcgcttcg tcgacggcat cgacaaggag ccgttcttcc agaagcgggc gcccgacaac
     1201 cggcccgagt ggatgcgcgt cgcgacgctg acgttcccgt cggggcggac ggcggacgag
     1261 atcgtggtcg acgaggcggc gggcctcgtc tgggtcgcga acctcggctg catcgacctc
     1321 aacccgcacc ccgtcagggc cgaggacatg gagcaccccg acgagctgcg cgtggacctc
     1381 gacccggtgc cgggcgtgcc gtggtcgcag gtgcgcgagg tcgccctggt cgtgcaggag
     1441 tcgctcgagg cggtcgggct tgtcgggtgg ccgaagacct ccggctcgcg cggcatccac
     1501 gtcaacgtcc ggatcgagtc gcgctggacg tactcgcagg tccggcgggc cgccctggcg
     1561 ctggcccgcg atgtggagcg ccgcgcgccg tcgatcgcca c
//
LOCUS       DBNHXO010000352         2538 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1885284, whole genome shotgun sequence.
ACCESSION   DBNHXO010000352 DBNHXO010000000
VERSION     DBNHXO010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1885284"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="AC162A_07285"
     CDS             complement(<1..167)
                     /locus_tag="AC162A_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trp family transcriptional regulator"
                     /protein_id="HIR5010682.1"
                     /translation="MADDWRTPDTEALIAALLRLETADEAGRFLRDLCTLGELRDLAQ
                     RWAVVRLLDAG"
     gene            274..1050
                     /locus_tag="AC162A_07290"
     CDS             274..1050
                     /locus_tag="AC162A_07290"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF013168.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR5010683.1"
                     /translation="MLGETGPLAVFELLPAIDIRGGRVVRLQQGDFERETAYGADPVE
                     VAARFVTQGATWLHVVDLDGARDGEPRQLEMAAAVVAEVHGRARVELGGGLRTPIAVA
                     GAIGTGAARVTVGTAALHDPEFVRSIVNRHGSGRVVASIDVRDGIALGEGWRPGATGM
                     PAAEAITMLATAGITTFEVTSIAHDGLLEGPDLELLRSLVALHSGRIIASGGVASIAD
                     VLAVQAIGCAGVIVGSALYEGRFDVAAAMAAVGNAGRRTV"
     gene            complement(1057..>2538)
                     /locus_tag="AC162A_07295"
     CDS             complement(1057..>2538)
                     /locus_tag="AC162A_07295"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR5010684.1"
                     /translation="DELADEIARRRDQLLEAAAEADDAVFEKYLGEEDISDAELDACL
                     HKGVRESVLAPVLVASAAKGIGLSALLDAIVRYLPSPEEEGPYAATDKAGKELEVPAT
                     GNQLLVRVFKTAADPFVGRLTYMRVLSGTLKSQGTVFNSSRGEDERIGQLLYLHGKEQ
                     EPAPELRAGEIGAVAKLGVTETGDTITTRDAALRLGPIDFPEPTLQVAIEPVSKGDLD
                     KMGPALQRMLEEEPTARVERSDTGEQILRAIGEAHVAVITERLKRKFGAAIATRTPTV
                     PYRETIRGNTKVHGRYKKQTGGHGMFGDVWIELEPNPGAGVAFAEKVVGGSVPRGFFA
                     GVEKGIRETAAEGVVAGFPLIDFRATLYDGSYHPVDSNELSFKIAASMALKDGVLQAK
                     PALLEPIMAVEVRVPEQYMGEVNRDLNGRRGRVLGLDSANGQQVISAHVPQAELFSYA
                     TELRSLTGGRGTFSSTLDHYEDVPTHLADKIIGEHKKDHGEGH"
BASE COUNT          327 a          904 c          911 g          396 t
ORIGIN      
        1 ttgccggcgt ccaggaggcg cacgacggcc cagcgctggg cgagatcgcg gagctcgccg
       61 agggtgcaca ggtcgcgcag gaaccggccg gcctcgtccg cggtctcgag ccggagcagg
      121 gccgcgatca gcgcctccgt gtcgggcgtc cgccagtcgt ctgccacgtc gtacgatgcc
      181 tccagtcagt tcgcgctggg ccaaggtcgc gtcgccgacc ttcctatcgt agtagcgtgc
      241 taatacgcta gcagccaggg ggaaccggtg tcaatgttgg gggagacagg cccgctcgcc
      301 gtgttcgagc tcctgccggc catcgacatc cgcgggggac gggtcgttcg gctccagcag
      361 ggcgacttcg aacgcgagac ggcttacggc gcggacccag tcgaggtcgc ggcccgcttc
      421 gtcacccagg gggcgacctg gctgcacgtc gtcgacctgg atggggcccg cgacggtgag
      481 ccacgccagc ttgagatggc cgccgccgtc gtcgcggagg tccatggccg cgcgcgggtc
      541 gagctcggcg gcgggctgcg gacgccgatc gcggtcgccg gtgcgatcgg gacgggcgcc
      601 gcgcgcgtca ccgtcggcac ggcggccctc cacgaccccg agttcgtccg ctcgatcgtc
      661 aaccgccacg gttcgggccg ggtcgtcgcc tcgatcgacg tccgcgacgg gatcgccctg
      721 ggcgagggct ggcggccggg cgccaccggg atgccggccg ccgaggcgat caccatgctc
      781 gcgaccgccg ggatcactac gttcgaggtg acatccatcg cccacgacgg gttgctcgag
      841 ggacccgatc tcgagctgct gcgctcgctg gtggccctcc acagcggccg gatcatcgcc
      901 tcggggggcg tggcctcgat cgcggacgtg ctcgcggtcc aggcgatcgg ctgcgcgggc
      961 gtgatcgtcg gcagcgccct gtacgagggg cgtttcgacg tcgccgcggc gatggcggcg
     1021 gtgggcaacg cgggacggcg aaccgtctga cgagggtcag tggccctcgc cgtggtcctt
     1081 cttgtgctcg ccgatgatct tgtcggcgag gtgcgtcggc acgtcctcgt agtggtcgag
     1141 ggtcgagctg aaggtgcccc ggccaccggt cagggaccgc agctccgtcg catacgagaa
     1201 cagctccgcc tgcggcacgt gcgcggagat gacctgctgg ccgttcgctg agtcgaggcc
     1261 caggacccgg ccgcgacgac cgttcaggtc gcggttgacc tcgcccatgt actgctcggg
     1321 cacgcggacc tcgaccgcca tgatcggctc gagcagggcc ggcttcgcct gcagcacgcc
     1381 gtccttgagc gccatggagg ccgcgatctt gaacgagagc tcgttcgagt cgacggggtg
     1441 gtacgagccg tcgtagagcg tcgcccggaa gtcgatcagc gggaacccgg cgacgacgcc
     1501 ctcggccgcc gtctcgcgga tccccttctc gacgcccgcg aagaacccgc gcggcacgga
     1561 gccaccgacg accttctcgg cgaacgccac tccggcgccc gggttgggct cgagctcgat
     1621 ccacacgtct ccgaacatcc cgtggccgcc ggtctgcttc ttgtaccggc catggacctt
     1681 cgtgttgccc cggatcgtct cgcggtaggg gaccgtgggc gtgcgggtcg cgatcgccgc
     1741 gccgaacttg cgcttgagcc gttcggtgat gacggcgacg tgcgcctcgc cgatcgcgcg
     1801 caggatctgc tcgccggtgt cgctccgctc gacgcgggcg gtcggctcct cctcgagcat
     1861 ccgctggagc gcgggaccca tcttgtcgag gtcgcccttc gacacgggct cgatcgccac
     1921 ctggagcgtg ggctcgggga agtcgatggg cccgaggcgg agcgccgcgt cgcgcgtcgt
     1981 gatcgtgtcg ccggtctcgg tcaccccgag cttggcgacg gccccgatct cgcccgcccg
     2041 aagctccggc gccggctcct gctccttgcc gtgaaggtac agcagctgcc cgatgcgctc
     2101 gtcctcgccg cggctggagt tgaacaccgt cccctggctc ttgagcgtgc ccgacagaac
     2161 gcgcatgtac gtcagccggc cgacgaacgg gtccgcggcg gtcttgaaca cgcgcacgag
     2221 gagctggttg cccgtcgccg ggacctcgag ctccttgccc gccttgtccg tcgccgcgta
     2281 cgggccctcc tcctcgggcg agggcaggta gcgcacgatg gcgtcgagca gggccgacag
     2341 cccgataccc ttggccgcgg acgccacgag caccggcgcg aggaccgact cgcgaacgcc
     2401 cttgtggagg caggcgtcga gctcggcgtc cgagatgtcc tcctcgccca ggtacttctc
     2461 gaacacggca tcgtccgcct ccgcggccgc ctcgagcagc tgatcccggc gacgcgcgat
     2521 ctcgtcggcg agctcgtc
//
LOCUS       DBNHXO010000353         2686 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2678899, whole genome shotgun sequence.
ACCESSION   DBNHXO010000353 DBNHXO010000000
VERSION     DBNHXO010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2678899"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="AC162A_07300"
     CDS             <1..457
                     /locus_tag="AC162A_07300"
                     /inference="COORDINATES: protein motif:HMM:NF020922.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2461 family protein"
                     /protein_id="HIR5010685.1"
                     /translation="YKTHLGASIPWVEGRGDPTGDAVGDGPHGNGAYFNFQPGEMYAG
                     GGMWMPEKARLDAFRRALVDEPGRVQAALGDPAFVAWFGGVQSHESLKRVPPGYPADH
                     PEAERLRWKDVVFGRRLTDDEVCSPALPDLLADGYATAVPVFRFLAGLA"
     gene            463..1776
                     /locus_tag="AC162A_07305"
     CDS             463..1776
                     /locus_tag="AC162A_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HIR5010686.1"
                     /translation="MTRGRSAQRRRPGAVLGLAAMLVVTLVACDPAPTITPSPSASDA
                     LVAPEPSASAGPPPEPGHELYGFVPYWEMDDGIADHLADAPLSTIGLFSVTQETDGSL
                     RTTAPGYQRITGDVGRRIIEGAHKRGQRVELVYTSFGEARNRRLLRREELQDGIITSL
                     VAMVGDLGLDGVNVDIEALDPGLIVAYSGFVGRLRAALVAADPDDQVSVATQANLLGA
                     TMAAGANQAGADRIFMMAYDYRTGASAPGATSPLARRDGSEKDLPWSLDLYATLGVPP
                     EKLLLGLPLYGVVWPVAGPEIGDAETGRGEAWILRSHVDLLRDPNAVPQRDEVESVEV
                     YALGSDGSVGPPPTESPAASPSDPTASSPPSTTPRATARPSAPPPDVRWHAVYVDSPE
                     TLATKLELARERGLVGSGFWAIGYERGLPGYTQLMRRFADDEPLR"
     gene            1932..2276
                     /locus_tag="AC162A_07310"
     CDS             1932..2276
                     /locus_tag="AC162A_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010687.1"
                     /translation="MPKAVSSGRLCEETLRAFDRLCELEQRRDAAITELTPRQRHRRL
                     LLERRARAEEWLVPDQLAATVLELVAVADRIDDPALALLWTDHLPVAVLAVMERRGFR
                     RRLADGDIFLAG"
     gene            complement(2277..>2686)
                     /locus_tag="AC162A_07315"
     CDS             complement(2277..>2686)
                     /locus_tag="AC162A_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HIR5010688.1"
                     /translation="APCPTMRRMHLIAVVAALVAAAIHLWFFVLESVQFTQPKVYARF
                     GLHSAEDAAIVRPMAFNQGFYNLFLAIGTVVGLVLVAAGQVDAGRAIVLFACATMVGA
                     GVVLLASNRRFLVGASIQALPPLVVLVVSLIAR"
BASE COUNT          376 a          959 c          989 g          362 t
ORIGIN      
        1 ctacaagacg cacctcgggg cgagcatccc gtgggtcgag ggccgcggcg atccgaccgg
       61 tgacgcggtg ggcgatggac cgcacggcaa cggggcgtac ttcaacttcc agccgggcga
      121 gatgtacgcg ggcggcggga tgtggatgcc ggagaaggcc aggctcgacg cattccgccg
      181 agccctggtc gacgaacccg ggcgcgttca ggcggccctc ggggacccgg ccttcgtcgc
      241 ctggttcggc ggcgtccaga gccacgagtc cctcaagcgc gtgccgcccg gctatcccgc
      301 cgatcacccg gaggccgagc gactccggtg gaaggacgtc gtgttcgggc gccgcctcac
      361 cgacgatgag gtctgctcgc cggcgctgcc ggacctgctc gcggacggct atgcgacggc
      421 cgtgccggtg ttccggttcc tcgcggggct cgcctgacgc tcgtgacccg cggccggagc
      481 gcgcaacgcc gccggccggg ggcagtcctc ggcctcgcgg ccatgctcgt cgtcacgctc
      541 gtcgcctgcg atccggcgcc gacgatcacg ccgagccctt cggcgtcgga cgcgctggtc
      601 gcgccggagc ccagcgcgtc ggccggccca ccgcccgagc ccggccacga gctgtacggg
      661 ttcgtgccgt attgggagat ggatgacggc atcgccgacc acctcgccga cgcgcccctc
      721 tcgaccatcg gcctgttcag cgtcacccag gagaccgacg gctcgctgcg gacgacggcg
      781 cccggctacc agcgcatcac cggcgacgtc ggccgccgga tcatcgaggg ggcccacaag
      841 cggggccagc gggtcgagct cgtgtacacg agcttcggcg aggcgcgaaa ccggcgcctg
      901 ctccgccgtg aggagcttca ggacgggatc atcacctcgc tcgtcgcgat ggtcggcgac
      961 ctcgggctcg acggcgtcaa tgtggacatc gaggcgctcg atccggggct gatcgtggcc
     1021 tacagcgggt tcgtgggccg cctccgcgcg gcgctcgtcg cggccgatcc ggacgatcag
     1081 gtctcggtcg cgacgcaggc caacctcctg ggggccacga tggccgccgg cgcgaaccag
     1141 gcgggggcgg accgcatctt catgatggcg tacgactacc ggactggcgc gtcggcgccg
     1201 ggcgcgacgt ccccgctcgc ccggcgcgac ggcagcgaga aggacctgcc gtggtccctc
     1261 gacctgtacg cgacgctggg tgtgccgccg gagaagctgc tgctcggcct gcccctctac
     1321 ggcgttgtct ggccggtcgc gggccccgag atcggggacg ccgagaccgg gcggggcgag
     1381 gcctggatcc tgcgcagcca cgttgacctg ctgcgcgatc cgaacgccgt tccgcaacgc
     1441 gacgaggtcg agtccgtcga ggtctacgcg ctcggctcgg acggctccgt cgggccgccg
     1501 cccaccgagt cgcccgcggc atcgcccagc gatccgacgg cctcgtcgcc accgtcgacg
     1561 acaccccggg cgaccgcacg gccgtccgcg ccaccgccgg acgttcgctg gcacgccgtc
     1621 tacgtcgact cacccgagac gctggccacg aagctggagc tcgctcgcga gcgagggctc
     1681 gtcggcagcg gcttctgggc catcgggtac gagcggggac tgcccgggta cacacagctc
     1741 atgcgccggt tcgccgacga cgagccgctg cgctaaggcc cgatcaccgg ccgttcgccc
     1801 ggcgcgagaa tcggcttcga cgtccgtctt cttcgaccta tgctgtggtg acaggacgca
     1861 ccacattcgt cgtcatcgcg agcgtgggcg ccaggggact gtgaccgcgg acgcggcggt
     1921 ggagggtgcg gatgccgaag gcggtgagct cgggccggct gtgcgaggag acgctgcgcg
     1981 cgttcgatcg cctgtgtgag ctggagcagc gtcgcgacgc tgccatcacc gagctcacgc
     2041 cgcggcagcg ccatcgccgc ctcctgctgg aacggcgcgc ccgggccgag gaatggctcg
     2101 ttcccgacca gctcgcggcc acggtgctgg agctggtggc ggtcgcggac cggatcgatg
     2161 atccggcgct cgccctcttg tggaccgatc acctgcccgt cgcggtgctg gccgtgatgg
     2221 agcgccgcgg gttccgccgg cgcctcgcgg acggcgacat cttcctcgcg gggtagtcag
     2281 cgggcgatca ggctcacgac gaggacgacg agcggcggca gcgcctggat cgacgcaccg
     2341 accaggaagc gccggttcga ggcgaggagc acgacgcccg cgcctaccat cgtggcacac
     2401 gcgaacagga cgatcgcgcg gcccgcgtcg acctggccgg ccgccacgag gacgaggccg
     2461 acgacggtcc cgatcgccag gaacaggttg tagaagccct ggttgaacgc catcgggcgc
     2521 acgatcgcgg cgtcctcggc cgaatggagc ccgaagcgcg cgtagacctt cggctgcgtg
     2581 aactggacgc tctccagcac gaagaaccac aggtggatgg cggccgccac gagggcggcg
     2641 acgacggcga tgaggtgcat gcggcgcatt gtggggcacg gcgcgg
//
LOCUS       DBNHXO010000354         4410 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1886021, whole genome shotgun sequence.
ACCESSION   DBNHXO010000354 DBNHXO010000000
VERSION     DBNHXO010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1886021"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="AC162A_07320"
     CDS             <1..441
                     /locus_tag="AC162A_07320"
                     /inference="COORDINATES: protein motif:HMM:NF025589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HIR5010689.1"
                     /translation="TRDNGRHVTHHRRPLPPVPPITELTRIEPVYLDRLEKQGVFTTG
                     ILLEVSETPTRRQYLADHVKADLTDVLAWRDEGLMLNLAGFGPDEHQLFIQAGIEGLR
                     DILETDLEPFRDRLGRAATELNWSPPADLMVDTWWEQARTLEDE"
     gene            455..1174
                     /locus_tag="AC162A_07325"
     CDS             455..1174
                     /locus_tag="AC162A_07325"
                     /inference="COORDINATES: protein motif:HMM:NF018964.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein intramolecular transacylase Lit"
                     /protein_id="HIR5010690.1"
                     /translation="MHTLTGAIASLVVGIAAALVIVAIAILPFLNPVWVSFEQGRAQA
                     AAWTGLSDADLRMATDAILADLVIGPPDFDVQLDGVPVLNDREQGHMRDVRGVFAAFY
                     GLAVVGAIVLVAAFLFARGREARVRLWRRLGLAGRVIAVATVALGIAGVMFFDTAFEV
                     FHELFFPAGSFLFDPSTERLVQLFPESFWVESTIAVGIVVVLLAVGLAWLGGRRARLL
                     EARVAGDGVVPMQPAAAEAGR"
     gene            1174..2310
                     /locus_tag="AC162A_07330"
     CDS             1174..2310
                     /locus_tag="AC162A_07330"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR5010691.1"
                     /translation="MGGGVPVARILGIEIRVSFAWAVLIALITVIGAQQATLGAPTIA
                     PVVQWAIGVAVALLFLVSVIAHELAHALVGRRRGVPTTVIALGFIGGLAPLAIQAPRA
                     RDELVIALVGPAVSLLLAALLIAAGVLLGTVVPESAPIAGGVVVVGVLNLILGVISLV
                     PAMPLDGGRVVRAVAWGRMGDRDRATSVTARVGRMVGWTIMGVGIALAFAEFVTEGLI
                     AIALGWLLNTGARTVERRLALEQLLRGLKVGEAMERDVPFVGPGLTIDTFASRFEGPD
                     AVTAMPVVEDDVVVGVLGRKRLMRLGRRRFGGTRVDEVMAVPPRAPFLAPSDALWDAV
                     ELLNEAGLDGLAVAEGGHLAGLVTRDGLANVIRLHAASRAATGG"
     gene            2315..3637
                     /locus_tag="AC162A_07335"
     CDS             2315..3637
                     /locus_tag="AC162A_07335"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR5010692.1"
                     /translation="MSSDLLRVDAAQATVLAGVEALADVELVPPELALGRVLAEPLVA
                     GVSLPPWDNAAMDGYAVRAADVAGATEARPVRLTVVGESRAGSAPDVRVLGATAVRIA
                     TGAPMPAGADAVVPVELTTPLNDAGNPGPRGREAAGPLPAACLVHEPVGGGNAVRERG
                     SDVEAGATVVAAGVTVTPAVVALAAGAGLAGIHVRRRPIVAVLATGDEVRAAGTDLGP
                     AGIPDANGPGIRALVREAGAVPIELGIAADRLEDVEARLRRGIAEADVVVVSGGVSVG
                     PYDVVRNAFDAVGHINLWRVAVQPGKPFAFGRADVDGRSHPVLLFGLPGNPVSSFVTF
                     ELFVRPVLRRLAGHPRIHRPADRAVLEEPVTKSVGRRGYQRVVVARDADGTPVRDGRG
                     RVSARLAGGQGSHVLSALAAADALAVIPEPLERADAGTEVELRWLDRG"
     gene            3673..4260
                     /gene="moaC"
                     /locus_tag="AC162A_07340"
     CDS             3673..4260
                     /gene="moaC"
                     /locus_tag="AC162A_07340"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR5010693.1"
                     /translation="MDTGDRTRAERRRLTHVDRRGRPRMVDVSAKPPTARRAVAEAEV
                     AVSAETMSLVIDGGGEKGDVLTVAEIAGVMAGKRTAELIPLCHPIPLTDLVVSISPDR
                     AAGVLRIHAEAATTGPTGVEMEAMTAASVAALTVYDMVKGVERGVEVRGLRLLSKTGG
                     KSGEWHRPASGDPGPRPRGKPGDGSAGRIGKRRGA"
BASE COUNT          495 a         1547 c         1734 g          634 t
ORIGIN      
        1 acccgtgata atggccgcca cgtcacccac catcggaggc cgcttccgcc cgtgccgccc
       61 atcaccgagc tcacgcgcat cgagcccgtg tacctcgacc ggctcgagaa gcagggcgtg
      121 ttcacgaccg ggattctgct cgaggtctcc gagacgccga cccgccggca gtacctcgcc
      181 gaccacgtga aggccgacct caccgacgtc ctcgcgtggc gcgacgaggg tctcatgctg
      241 aacctcgccg ggttcggtcc cgacgagcac cagctgttca tccaggccgg gattgagggc
      301 ctgcgcgaca tcctcgagac ggacctcgag ccgttccgcg accggctcgg gcgcgccgcg
      361 acggagctca actggtcgcc tccggcggac ctgatggtcg acacctggtg ggagcaggcc
      421 cggacgctcg aggacgaata gcctcggggc caccatgcac accctcaccg gcgccatcgc
      481 atcgctcgtc gtcggcatcg ccgcggcgct cgtcatcgtg gcgatcgcga tcctgccgtt
      541 cctgaacccg gtctgggtct cgttcgagca gggccgggcg caggcggcgg cgtggaccgg
      601 cctctccgac gcggacctcc ggatggcgac cgacgcgatc ctcgcggacc tcgtgatcgg
      661 accgcccgac ttcgacgtcc agctcgacgg cgtccccgtg ctcaatgatc gcgagcaggg
      721 ccacatgcgc gacgtgcgag gcgtgttcgc ggcgttctac gggctcgcgg tcgtcggggc
      781 gatcgtgctg gtggcggcgt tcctgttcgc gaggggtcgc gaggcacgtg tgcgcctgtg
      841 gcggcggctc gggctcgccg gcagggtgat cgccgtcgcc accgtcgcgc tgggcatcgc
      901 gggggtgatg ttcttcgaca cggcgttcga ggtgttccac gagctgttct tcccggccgg
      961 ttcattcctg ttcgacccga gcacggagcg cctggttcag ctgttccccg agtcgttctg
     1021 ggtcgagtcg acgatcgccg tcgggatcgt ggtggtgctg ctcgcggtcg ggctggcgtg
     1081 gctcggcggg cgccgggcgc ggctcctgga ggcgagggtc gcgggcgacg gcgtcgttcc
     1141 gatgcagcca gcagccgccg aggcgggccg ctgatgggtg gcggggtgcc ggtcgcgcgg
     1201 atcctgggca tcgagatccg cgtgtcgttc gcctgggcgg tgctcatcgc cctgatcacc
     1261 gtcatcggcg cccagcaggc cacgctgggc gcccccacga tcgcgccggt cgtccagtgg
     1321 gcgatcgggg tcgccgtggc gctcctgttc ctcgtgtcgg tgatcgcgca cgagctcgcg
     1381 catgccctgg tcgggcggcg gcgcggcgtc cccacgaccg tgatcgcgct cggcttcatc
     1441 ggcggcctcg cgccgctggc gatccaggcg ccgcgggcgc gggacgagct ggtcatcgcc
     1501 ctcgtcggcc cggcggtgtc gctccttttg gccgcgctcc tgatcgcggc cggcgtcctg
     1561 ctcggcacgg tcgtgcccga gagcgccccg atcgccggcg gcgtcgtggt ggtcggcgtc
     1621 ctcaacctga tcctgggggt catcagcctc gtgccagcga tgccgctcga cggcggtcgc
     1681 gtggtccggg cggtcgcctg gggccggatg ggcgaccggg atcgcgcgac cagtgtcacc
     1741 gcgcgcgtcg gccggatggt cggctggacg atcatgggcg tggggatcgc cctcgccttc
     1801 gcggagttcg tgaccgaggg gctgatcgcg atcgcactgg gctggctgct caacacgggt
     1861 gcgcggacgg tggagcgacg cctggcgctg gagcagctcc tgcggggact caaggtcggt
     1921 gaggcgatgg agcgcgacgt gccgttcgtc gggcccggcc tcacgatcga cacgttcgct
     1981 tctcgcttcg agggacccga cgcggtgacc gcgatgccgg tggtcgagga cgacgtggtc
     2041 gtcggcgtcc tcggtcgcaa gcggctgatg cgactcggac gacggcggtt cggcgggacc
     2101 cgggtggacg aggtgatggc cgtgccgccg cgggcgccat tcctcgcgcc gtccgatgcg
     2161 ctgtgggacg ccgtcgagct gctcaacgag gcgggcctcg acggcctggc ggtcgccgag
     2221 ggcggccacc tcgcgggcct cgtgacccgc gatgggctcg cgaacgtgat ccggctccac
     2281 gcggcgtccc gtgccgcgac gggcggctga gggcgtgagc agcgacctgc tgcgcgtcga
     2341 cgctgcccag gcgaccgtcc tggcgggcgt cgaggccctg gccgacgtcg agctcgtgcc
     2401 gcccgagctg gcgctcggcc gggtgctggc cgagcccctg gtcgccggcg tctcgctgcc
     2461 gccctgggac aacgcggcga tggacgggta cgcggtgcgc gcggccgacg tcgcgggtgc
     2521 caccgaggcc cggcccgtgc gcctgacggt ggtcggcgag tcgcgggccg gatcggcgcc
     2581 cgatgtccgg gtgctcgggg cgacggcggt ccggatcgcg accggggcgc cgatgcccgc
     2641 gggcgccgat gcggtggtgc cggtcgagct caccacgccg ctcaacgacg ctggcaaccc
     2701 gggtccccgg ggccgcgagg cggccggccc gctgccggct gcctgcctcg tccacgagcc
     2761 ggtgggtggc ggcaacgccg ttcgcgaacg cggcagcgac gtcgaggccg gggcgacggt
     2821 cgtcgctgcc ggcgtcaccg tgacgccggc ggtggtcgcg ctcgccgcgg gcgctgggct
     2881 ggccggcatc cacgtgcgcc gtcgcccgat cgtggcggtc ctcgcgaccg gcgatgaggt
     2941 gcgcgcggcc gggaccgacc tcgggcctgc ggggatcccg gacgcgaacg gcccgggtat
     3001 ccgtgcgctc gtccgcgagg ccggcgcggt cccgatcgag ctcggcatcg ccgcggatcg
     3061 cctggaggac gtcgaggcgc ggctgcggcg cggcatcgcc gaggccgacg tcgtcgtcgt
     3121 gtcgggcggg gtgtcggtcg ggccgtacga cgtcgtacgg aacgcattcg acgccgtcgg
     3181 gcacatcaac ctgtggcgcg tcgccgtgca gccgggcaag ccgttcgcct tcggccgcgc
     3241 cgacgtcgac ggcaggtcgc acccggtgct cctgttcggg ctgccgggca acccggtgtc
     3301 gtcgttcgtg accttcgagt tgttcgtccg gcccgtgctg cgccgcctgg ccgggcatcc
     3361 gcggatccac cgaccggccg accgcgcggt gctcgaggag ccggtgacga agagcgtcgg
     3421 gcgccgcggg taccagcgcg tcgtggtggc gcgggacgcg gacgggaccc cggtgcgcga
     3481 cgggcgcggg cgagtcagcg cgcgcctcgc gggcgggcag gggagccacg tcctctcggc
     3541 cctggccgcc gccgatgccc tggcggtcat cccggaaccg ctcgagcggg cggatgcggg
     3601 caccgaggtg gagctgcgct ggctcgaccg cgggtagctc gcccggctag catcgtcggt
     3661 gcaggagggt ccatggacac tggcgaccgc acgcgcgccg aacggcgacg cctcacccac
     3721 gtggaccgcc gcgggcggcc gcggatggtg gacgtcagcg ccaagccgcc gaccgcgcga
     3781 cgcgccgtcg cggaggccga ggtcgcggtg tcggccgaga ccatgagcct cgtcatcgac
     3841 ggtggcggcg agaagggcga cgtgctgacg gtcgcggaga tcgccggcgt gatggccggc
     3901 aagcggaccg ccgagctgat cccgctgtgc cacccgatcc cgctgaccga cctggtcgtc
     3961 tcgatctcgc cggaccgggc cgccggggtg ctgcgtatcc acgccgaggc cgcgacgacc
     4021 ggcccgacgg gcgtcgagat ggaggcgatg accgctgcct ccgtggccgc gctgaccgtc
     4081 tacgacatgg tcaagggcgt cgagcgcggg gtcgaggtcc gggggctgcg gctgctctcg
     4141 aagaccgggg gcaagagcgg cgagtggcac cggccggcgt cgggcgaccc agggccgcgg
     4201 ccgcgcggca agcccgggga cgggtccgcg ggccggatcg gcaagcgcag aggggcctga
     4261 tgcccgatcc gtccgcgctc gtcctgaccg tgagcgaccg gagcgccgcg ggcacgcgcg
     4321 aggatgcatc gggggcggcg ctggagaccc ggctggtcga gctcgggttc accgtcgaac
     4381 gtcgggtcgt gccggacgag cgctgggaga
//
LOCUS       DBNHXO010000355         2934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3670872, whole genome shotgun sequence.
ACCESSION   DBNHXO010000355 DBNHXO010000000
VERSION     DBNHXO010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3670872"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..738
                     /locus_tag="AC162A_07345"
     CDS             277..738
                     /locus_tag="AC162A_07345"
                     /inference="COORDINATES: protein motif:HMM:NF019499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HIR5010694.1"
                     /translation="MTSTVARLRGIHPGWILVVLDLVALAIAGYLASVELGGGVPYCG
                     ALHGCETVAQSEYSRIAGIPVAVYGVVASLVLLALALAWIRTDRAVLLDLSYGLSLIG
                     VLFEAYFVFLQVFVIHALCIWCSAYGLTMVARLGVTWWARRSRGRDAGRTP"
     gene            complement(748..1584)
                     /locus_tag="AC162A_07350"
     CDS             complement(748..1584)
                     /locus_tag="AC162A_07350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR5010695.1"
                     /translation="MRYGIVLTTGDPRTAAELAADAEAAGWDGVFTFDAIAIGPVPMY
                     DPWIVLAAMAMRTQRVILGAIVFAPSRRRPWKLAREAISLDLLSNGRLVLPVGLGALD
                     DAGFGNVGEDTSPGVRARRLDETLAILNGLESGEPFAFEGEHYRFGPMTFQPRPVQRP
                     RIPVWVVGAWPHERSMRRAARWDGIVVQALGPEGDPATGPEVLPPIVDWLRRERPADL
                     RDRPFEIVVDGVTPGDDPDAAAAITRAHEAAGATWWIESDWSAAGVESLRARIVAGPP
                     RG"
     gene            1792..2229
                     /locus_tag="AC162A_07355"
     CDS             1792..2229
                     /locus_tag="AC162A_07355"
                     /inference="COORDINATES: protein motif:HMM:NF016221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HIR5010696.1"
                     /translation="MRTRIALLLLMLAVVAACAGPRGSRSGVVSVGDAWARPGAAGAT
                     SAAYLRITNGTPDGDTLVGASTPAATTASIHQTVTDDSGMTGMHAATLTVPAGETVAL
                     EPGGYHVMLTGLAADLAPGSEIQLTLTFERAGPLVVPVQVRGS"
     gene            2237..>2934
                     /locus_tag="AC162A_07360"
     CDS             2237..>2934
                     /locus_tag="AC162A_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR5010697.1"
                     /translation="MARPGDTVPDPRPAPVDRRALLAGGIVLLGFAFVAASLFGLVLL
                     ARNDTATAGTGGPRPAPPLELTDQDGRPFALTSLLGRPALVFFGYTSCPDVCPQTVGI
                     ISQAMSASPPGARAVFVSIDPERDDVAAMKAYLQYLPATFTGLTGTPDEVRRNADAWS
                     VRYAKEVDDAALGGYGMAHTADIFLVDAAGNVQTQFRYGTTAETIAAALTPLLVARPV
                     PTDPEPSPVATPAST"
BASE COUNT          386 a         1076 c         1064 g          408 t
ORIGIN      
        1 gcggcgagcg ccccacctgc ctggacgatc aggagcgcga atgcggccaa gaccgcgagc
       61 tggcgacgcg ccacggcgag accctcctcg aaccgcccgg acaagcgcat tccatgacgt
      121 gggtcagcag gccgggcacg acgaccaaac cagaggcagc gggggaccgg agcgtacacc
      181 gccgcgcaag cgaaggaacg cccaacctgc gcgatttgtt gcgccggccc ctgcgcacgc
      241 gtcgcgcggc ctcgggctat gctcgggcgc gttccgatga cctcgaccgt cgcccgtctc
      301 cgcggcatcc acccgggctg gattctcgtc gtcctcgacc tcgtcgccct ggccatcgcc
      361 ggatacctgg cgagcgtcga gctgggcggc ggcgtcccgt actgcggggc cctgcacggt
      421 tgcgagaccg tcgcccagag cgagtactcg cggatcgccg ggatccccgt ggcggtctac
      481 ggcgtcgtcg cgtcgctggt gctgctcgcc ctggccctcg cctggatccg caccgaccgg
      541 gccgtgctgc tcgacctcag ctacgggctg tcgctcatcg gggtcctgtt cgaggcgtac
      601 ttcgtgttcc tgcaggtgtt cgtgatccac gcgctgtgca tctggtgctc cgcgtacggc
      661 ctcacgatgg tcgcgcggct gggggtcacc tggtgggcca ggcgctcacg tgggagggac
      721 gccggccgca cgccttgagc gtcggcgtca gccgcgggga ggcccggcca cgatccgtgc
      781 tcggaggctc tcgacgccgg ccgccgacca gtccgactcg atccaccagg tggcgccggc
      841 agcttcgtgg gcacgggtga tggccgcggc agcgtccgga tcgtcgccgg gtgtcacccc
      901 gtcgaccacg atctcgaacg ggcggtcgcg gaggtcggcg ggccgctcgc ggcgcagcca
      961 gtcgacgatc ggcggcagga cctcgggtcc cgtggcgggg tcaccctcgg gtcccagcgc
     1021 ctggacgacg atcccgtccc agcgcgcggc ccggcgcatc gagcgctcgt gcggccaggc
     1081 ccccaccacc cagacgggga tccgcggccg ctggaccggc cggggttgga aggtcatcgg
     1141 cccgaaccgg tagtgctctc cctcgaacgc gaacggctca cccgactcga ggccgttgag
     1201 gatggcgagc gtctcgtcga gccggcgggc gcgaactccg ggcgacgtgt cctcgcccac
     1261 gttcccgaag ccggcatcgt cgagcgcgcc gagacccacc gggagcacga ggcggccgtt
     1321 cgagagcaga tcgagcgaga tggcctcccg ggccagcttc cacggcctgc ggcgggaggg
     1381 cgcgaacacg atcgcgccga ggatgacgcg ctgggtccgc atcgccatcg cggccagcac
     1441 gatccacggg tcgtacatcg gaaccggtcc gatcgcgatc gcgtcgaacg tgaagacgcc
     1501 gtcccagccg gccgcctccg catcggccgc cagctcggcg gcggtccggg ggtcgccggt
     1561 cgtgagcacg atgccgtagc gcatcgtcag atcatcaccg agtcgacgtc gcggcgcggc
     1621 ggtcaccgtc gggctgctga tcgggtacct cgcctggcga gcctggcagg cggtcgtggc
     1681 ccggtccgag cggcctgcct gattcgggcc gttgcgcccg acgcccggaa tcgattgaac
     1741 tgcgagactg ggtctgatcg accacgccgg tcgacccgct cgaggccgtc gatgcgaacc
     1801 cggattgccc tgctgctgct catgctcgcg gtcgttgccg cgtgcgccgg gccgcgcggc
     1861 agccgcagcg gcgtcgtctc ggtcggcgac gcctgggccc gacccggggc cgcgggcgcg
     1921 accagtgcgg cctacctgcg gatcacgaac ggcacccctg acggcgacac gctggtcggc
     1981 gcgtcgacgc cggccgcgac gacggcatcg atccaccaga cggtcaccga cgacagcggc
     2041 atgacgggga tgcacgcggc gacgctgacc gtccccgcgg gcgagacggt ggccctcgag
     2101 cccggcggct accacgtgat gctgaccggg ctggcggccg acctcgcgcc gggatcggag
     2161 atccagctca cgctgacctt tgagcgggca ggcccgctcg tcgtgcccgt ccaggttcgc
     2221 gggagctgag ggcgcgatgg cccgtcctgg ggacaccgtg ccagacccgc gtcccgcgcc
     2281 ggtcgaccgg cgggcgctcc tggccggcgg gatcgtgctg ctcgggttcg ccttcgtcgc
     2341 ggcgagcctg ttcggcctcg tgctgctcgc gcgcaacgac accgcgacgg caggcacggg
     2401 cggtccccga cccgccccgc cgctcgagct caccgaccag gatgggcggc cgttcgcgtt
     2461 gacgtcgctg ctcgggcggc ccgcgctcgt gttcttcggc tacacgagct gccccgacgt
     2521 ctgcccgcag acggtgggga tcatcagcca ggcgatgtcg gccagcccgc ccggggcgcg
     2581 ggcggtgttc gtgtcgatcg atcccgagcg cgacgatgtg gctgcgatga aggcctacct
     2641 gcagtacctg ccggcgacct tcaccggact cacggggaca ccggacgagg tccgtcgcaa
     2701 cgccgacgcc tggtcggtgc gctatgcgaa ggaggtcgac gatgcagcgc tcggcggcta
     2761 cggcatggcc cacacggcgg acatcttcct ggtcgatgcg gcgggcaatg tgcagacgca
     2821 gttccggtac ggcaccacgg ccgagacgat cgcggctgcg ctgacgccgc tgctggtcgc
     2881 ccggcctgtc ccgacggacc cggagcccag cccggtcgcg acacccgcct cgac
//
LOCUS       DBNHXO010000356         4378 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4467696, whole genome shotgun sequence.
ACCESSION   DBNHXO010000356 DBNHXO010000000
VERSION     DBNHXO010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4467696"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="AC162A_07365"
     CDS             complement(<1..1342)
                     /locus_tag="AC162A_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5010698.1"
                     /translation="MAPAPATPAELLASPVGLSGITQVAGLRRVARRMGIDTVRDLLF
                     HLPRRYDDLRELRMLGDLRDADDGTVASARVTVTDISVQQTWRRRVQVTTAHVRDATG
                     FATVTWFGRRYIERRLATGDEILVSGKIKHRHGEVVFEGPEFQPVDAANLLHVGRIVP
                     VYRLTSGVTANRFRGAMRVALDRAGDEYPEYLPPAILAAEGVPPIGEALEAAHYPTTF
                     EARDAALHRLALDELLALQLGMVSRRRARGRARTLAIATDEAADGRIRAALEASLERK
                     LGEPAALTADQATAIDEIAADLARPIPMLRLVQGDVGSGKTAVAAWALAAAALDGRQA
                     ALLAPTDLLARQHHVTLTSLLEDLGLPITLLTGSLSGEGRRNAGQALASGQAAIVVGT
                     HALLQESVAFADLALAVVDEQHRFGVEQRGALEAKATRGAPHVLLMTATPIPRTL"
     gene            complement(1405..3120)
                     /locus_tag="AC162A_07370"
     CDS             complement(1405..3120)
                     /locus_tag="AC162A_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIR5010699.1"
                     /translation="MAARSCDGAGFLAAFRSAVASLEANVEEINALNVFPVPDGDTGT
                     NMLATVRAALEEADRAGPDADVERVAHAASYGALMGARGNSGVITSQILAGMAHGLAG
                     KRRFNGLDLAHALDEGTKTAYKAVAKPVEGTILTVIREASAAAVAAAERDNNVETVLA
                     ATLEAAERAVAKTPSLLPILREAHVVDSGGQGLFRLFQGALDAARGRPVKGGGKAVAP
                     PVHGVPAPEPVAGAEEGEFGYETVYLLRARPGAPLDIPAIQAYLEAMGDSVLVAGDAL
                     LAKVHVHNERPDAVIAYGLSIGTLSRITIENLDSQAHDVRESKAAAFVGAADSADGSN
                     GADPTADVPATRLALGVVVVAPSDGLAAVMTDTAAPFREYGAFRVVKGGQSANPSTGE
                     LLEAVEATDADELLILSNNPNVVLAARQVAAMTQRTIRVVPTRNCAEGVAALFELDPT
                     QGAEANAATMTEAGRAIQTMQVTEAIRDATVSGRKVKKGQTIVLDPDDGLLAVDNDPQ
                     KAVMAGLARLDPGYGLLTIYYGETATLDDAETLARKVREVAPGVDVEVVHGGQPHYRY
                     LVSAE"
     gene            complement(3120..3542)
                     /locus_tag="AC162A_07375"
     CDS             complement(3120..3542)
                     /locus_tag="AC162A_07375"
                     /inference="COORDINATES: protein motif:HMM:NF015721.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR5010700.1"
                     /translation="MSAPMSGRSLVTRRAIRDLVRTATLSTYGVTGFSGGGPLGGLLG
                     RLGIAHPGLRLDVGESLTVDLDLTVAYGLPVAEVARQVDASVRYALADALGREPDRIS
                     IRIGHLVHEPGHAPGMATDAHGHEPRPADLAGSGTDVA"
     gene            3704..3901
                     /locus_tag="AC162A_07380"
     CDS             3704..3901
                     /locus_tag="AC162A_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013026.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large ribosomal subunit protein bL28"
                     /protein_id="HIR5010701.1"
                     /translation="MARSCAICGKTSMGGFNPQSSGMNRVRAHRRYQPNLQVTTLTKD
                     RTTTKVLACTRCRRTLTKNAR"
     gene            complement(4170..>4378)
                     /locus_tag="AC162A_07385"
     CDS             complement(4170..>4378)
                     /locus_tag="AC162A_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013068223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR5010702.1"
                     /translation="LYADTSRGRRPGFTDAAEPDLARPLWLRVAGAIEAQGVRTALGE
                     FGAEMAVELVNDGPFTIWLDTADR"
BASE COUNT          581 a         1609 c         1593 g          595 t
ORIGIN      
        1 cgagggtgcg cgggatcggc gtcgcggtca tcagcagcac gtgcggggcg ccgcgggtcg
       61 ccttcgcctc gagcgccccg cgctgctcga cgccgaaccg gtgctgctcg tcgaccaccg
      121 ccagcgccag gtcggcgaac gcgacggact cctggagcag cgcgtgcgtc cccaccacga
      181 tcgccgcttg ccccgacgcc agcgcctggc ccgcattccg ccgcccttcg ccggagagcg
      241 aaccggtcag cagcgtgatc ggcagcccca ggtcctcgag cagcgaggtc agcgtgacgt
      301 ggtgctgacg cgcgagcagg tccgtcggcg ccaggagcgc ggcctggcgg ccgtcgagcg
      361 ccgcggccgc gagggcccac gccgccaccg ccgtcttgcc cgacccgacg tcgccctgga
      421 cgagccgcag catcgggatg ggccgcgcga ggtcggccgc gatctcgtcg atcgccgtcg
      481 cctggtccgc cgtgagcgcc gccggctcgc ccagcttgcg ctccagcgac gcctcgagcg
      541 ccgcccggat ccgcccgtcc gcggcttcgt cggtggcgat cgccagcgtc ctcgcccgac
      601 cccgcgcgcg ccgccgggac accatcccca gctggagcgc gagcagctcg tccagcgcga
      661 gccggtggag cgcggcgtcg cgcgcctcga aggtggtggg gtagtgggcc gcctcgaggg
      721 cctctccgat cggcggcacg ccctccgcgg cgaggatcgc cggaggcagg tattcggggt
      781 actcgtcgcc ggcgcgatcg agcgcgaccc gcatcgcgcc gcggaaccgg ttcgccgtca
      841 cgccgctggt caaccggtac acggggacga tgcgcccgac gtgcagcagg ttggcggcgt
      901 cgacgggctg gaactccggc ccctcgaaca cgacctcgcc gtggcggtgc ttgatcttgc
      961 ccgagaccag gatctcgtcg ccagtcgcga ggcgacgctc gatgtaccgc cgtccgaacc
     1021 aggtgaccgt ggcgaagccg gtcgcatcgc gcacatgcgc cgtcgtgacc tggacgcggc
     1081 ggcgccaggt ctgctggacc gagatgtcgg tcacggtgac ccgggccgag gccaccgtcc
     1141 cgtcgtcggc gtcgcggaga tcgcccagca tccggagctc gcgcaggtcg tcgtagcgcc
     1201 gcggcaggtg gaacagcagg tctcgcaccg tgtcgatgcc catgcggcgg gcgacccggc
     1261 ggagtcccgc gacctgggtg atgcccgaca gcccgaccgg cgacgcgagc agctcggcgg
     1321 gcgtcgcggg cgcgggcgcc acgaccttcg gccggagcga gcggctgcca ctcgccgggg
     1381 cggtgcgtcg gcgctcggcg gggctcactc ggcggagacg aggtaccggt agtgcggctg
     1441 gccgccgtgg accacctcga cgtccacgcc cggcgcgacc tcgcggacct tgcgcgccag
     1501 ggtctcggcg tcgtcgaggg tcgccgtctc gccgtagtag atcgtcagca ggccatagcc
     1561 cgggtcgagc cgcgcgagtc cggccatgac cgccttctgg gggtcgttgt ccacggcgag
     1621 gaggccatcg tcgggatcga gcacgatcgt ctggcccttc ttcaccttgc ggcccgacac
     1681 cgtcgcgtcg cggatggcct cggtcacctg catcgtctgg atcgcccggc cggcctcggt
     1741 catcgtcgcc gcgttggcct cggcaccctg cgtcggatcg agctcgaaca gggcggctac
     1801 cccctccgcg cagttgcggg tcgggaccac gcggatcgtg cgctgggtca tcgcggcgac
     1861 ctggcgcgcc gccagcacga cgttggggtt gttggacagg atcaggagct cgtcggcatc
     1921 ggtcgcctcg acagcctcaa gcagctcgcc ggtcgacggg ttggcgctct ggccgccctt
     1981 gacgacgcgg aacgcgccgt actcgcggaa cggcgccgcg gtgtcggtca tgaccgccgc
     2041 gagaccgtcc gacggcgcga cgacgacgac ccctagggcc aggcgggtag cgggcacgtc
     2101 cgcggtggga tcagcgccgt tggagccgtc ggccgagtca gcggcaccca cgaacgcggc
     2161 cgccttcgac tcgcgcacgt cgtgcgcctg gctgtcgagg ttctcgatcg tgatccggga
     2221 cagggtcccg atcgacaggc cgtacgcgat gaccgcgtcc gggcgctcgt tgtggacgtg
     2281 gaccttggcc aggagcgcat ccccggcgac cagcaccgag tcgcccatcg cctcgaggta
     2341 cgcctggatc gcggggatgt cgagcggcgc gcccggccgg gcacggagca ggtagacggt
     2401 ctcgtacccg aactcgccct cttcggcgcc ggccacgggc tccggggcgg gcactccgtg
     2461 gacgggcggg gccacggcct tgccgccacc cttgaccggg cgaccccggg cggcatcgag
     2521 cgcgccctgg aacaggcgga acaggccctg cccgccggag tcgacgacat gcgcctcgcg
     2581 caggatcggc agcagggacg gggtcttggc gacggcgcgc tcggcggcct cgagcgtggc
     2641 ggcgagcacc gtctcgacgt tgttgtcgcg ctcggcggcg gcgaccgcgg cggccgacgc
     2701 ctcgcggatc accgtgagga tcgtgccctc gaccggcttc gccacggcct tgtaggccgt
     2761 cttcgtgccc tcgtcgagcg cgtgggcgag gtcgagtccg ttgaaccgcc gcttgccggc
     2821 gaggccatga gccatccccg ccaggatctg gctcgtgatc acgcccgagt tgccacgcgc
     2881 gcccatgagc gcgccatagc tcgcggcgtg cgccacccgc tcgacatccg cgtcggggcc
     2941 cgcccgatcg gcctcctcga gcgccgccct gaccgtcgcc agcatgttcg tccccgtgtc
     3001 gccgtcgggg accgggaaca cgttgagcgc gttgatctcc tcgacgtttg cttcgaggct
     3061 cgcgaccgcg ctgcggaagg ccgccaggaa cccggctccg tcgcaggatc gggcggccat
     3121 caggcgacgt ccgtgccgct gccggcgagg tcggccggcc ggggctcgtg gccatgggcg
     3181 tccgtcgcca tccccggcgc atgacccggc tcgtggacga ggtgcccgat gcggatcgag
     3241 atccggtccg gctcccgccc gagcgcgtcg gcgagggcgt agcggaccga cgcgtcgacc
     3301 tggcgcgcga cctcggcgac gggcagaccg taggcgaccg tcaggtcgag gtcgaccgtc
     3361 agcgactcac cgacgtcgag gcgcagcccc ggatgcgcga tgcccaggcg cccgagcagc
     3421 ccgccgaggg gtccaccgcc cgagaagccg gtgacgccat acgtcgacag ggtggcggtg
     3481 cggacgaggt cgcggatggc ccgccgggtg accagcgatc gaccggacat cggggcggac
     3541 acgcgggcga cgtccctcct ggcgggtgct tgtccagcct cgactcaggg ccggctcgcc
     3601 gggccggccg ggccggaggc gtgtgctacg ataccagccc gctcgctccg gaccctgttc
     3661 cgtgcgagtt cgtaacggcc gattccgagg atccaacgag accatggctc gaagctgtgc
     3721 catctgtggc aagacgtcga tgggtgggtt caacccgcag tcgtcgggga tgaaccgcgt
     3781 ccgcgcacat cgtcgctacc agccgaacct ccaggtcacg acgctgacca aggacaggac
     3841 caccaccaag gtccttgcct gcacccgctg ccgtcgaacc ctgaccaaga acgcccgcta
     3901 gccacccacg ccgggccaga accgggagcc ggtcgcgatc gaagcgaccg gcttcgtcgt
     3961 gttcggggct gcccgcgtcc gacggatcgg cccgaccgtt agcgatgacc aacgtgtgcg
     4021 ttcagctcac gctaccgtcg cgacgcatcg gcgggaccga tcccgccggt cgtagaggcg
     4081 cgacgggggc gccgttaccc ggcgcggggc catgatcggg tctcgctaac ggccccaccg
     4141 atctcggggc gccgaggagt ctggatcgat caacgatccg ccgtgtcgag ccagatcgtg
     4201 aacggaccgt cattcacgag ctccacggcc atctcggcac cgaactcgcc aagggcggtc
     4261 cggacgccct gggcctcgat tgcgcccgcg acccggagcc acagcggacg cgcgaggtcg
     4321 ggctccgcgg catccgtgaa cccgggtcgg cgtcctcggc tcgtgtccgc gtagaggg
//
LOCUS       DBNHXO010000357         1779 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2285813, whole genome shotgun sequence.
ACCESSION   DBNHXO010000357 DBNHXO010000000
VERSION     DBNHXO010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2285813"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1779)
                     /locus_tag="AC162A_07390"
     CDS             complement(<1..>1779)
                     /locus_tag="AC162A_07390"
                     /inference="COORDINATES: protein motif:HMM:NF015644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HIR5010703.1"
                     /translation="AALILAIGAILLLLVAGINLWTDAIWYRSVGYDSVFWTRLGVQA
                     GLFIGALVLALVVLLGNMVIANRMAPPADPERPGQLRVVAGRLAEAQRQAERSARSRA
                     STGPFGSLGVSGGRQGTGELTFSWEGEEIPDLVPIGTWVIAGVAVLLALGIAGAVTGA
                     WETLMLWLHRVPFDPTQSVTDPIFGRDVSFFLFELPFFRFVQALANGLLLASLLVVGA
                     RYLLAVTRGGEVFVTRVRVHLAVLGGLYLISVAFGYQLDKYELVYSSAGVATGVSYAD
                     ANARFMAYDVLTFLSGLAGALLIAGAFTRWMWPLGAIVIIWFSASIVLGRLYPEAIQR
                     LSVDPNTYAQEEPYIGNNISMTRLAFGLNAWESRSYGGAAPLTEAAIRSEADTFTNAR
                     LWDYRPLQTTLDQLQTVRQYYDFHDVDTDRYLVNGDLRQVMLSGRELAIDRSTDAPNW
                     VNTRVIKTHGIGVAMVPVNEVTPEGQPTLWIRDLPPTSNSGAPEITQPRIYFGESDDH
                     YVVVRAKQQEFDYPSNSNTGEPDPTTSWTGQTGIALDSTLSRLLFAIRFRDLDLLITD
                     QIQADSQLLFHRTLSERLGLIAPFLR"
BASE COUNT          301 a          588 c          639 g          251 t
ORIGIN      
        1 agcgcaggaa cggcgcgatc agccccagcc gctccgacag cgtccggtgg aacagcagct
       61 ggctgtcggc ctggatctgg tcggtgatca gcaggtcgag gtcgcggaac cggatcgcga
      121 acagcagccg cgacagcgtc gagtcgagcg cgatgccggt ctgcccggtc cacgaggtcg
      181 tcgggtcggg ctcgcccgtg ttcgagttgc tcgggtagtc gaactcctgt tgcttcgcgc
      241 ggaccacgac gtagtggtcg tcgctctcgc cgaagtagat gcgcggctgc gtgatctcgg
      301 gcgcgccgct gttggaggtt ggcggcaggt cgcggatcca gagcgtcggc tggccctcgg
      361 gggtgacctc gttgaccgga accatcgcga cgccgatgcc gtgggtcttg atgacccggg
      421 tgttgaccca gttcggcgcg tccgtgctgc ggtcgatcgc cagctcgcgc cccgacagca
      481 tcacctggcg caggtcgccg ttgacgaggt agcggtcggt atcgacgtca tggaagtcgt
      541 agtactggcg caccgtctgg agctggtcga gggtcgtctg cagcggccgg tagtcccaca
      601 ggcgcgcgtt cgtgaacgta tccgcctcgc tgcggatcgc cgcctcggtc agcggcgcag
      661 caccgccgta ggagcgggac tcccacgcgt tgaggccgaa ggccagccgc gtcatcgaga
      721 tgttgttccc gatgtacggc tcctcctggg cgtaggtgtt ggggtcgacg ctcaagcgct
      781 ggatggcctc gggatagagg cgtccgagga cgatggatgc cgagaaccag atgatcacga
      841 tcgcgccgag gggccacatc cagcgcgtga acgccccggc gatcagcagc gcgccggcca
      901 gcccggacag gaaggtgagc acgtcgtagg ccatgaatcg ggcgttcgcg tcggcgtagg
      961 agacgccggt ggccacgccg gccgagctgt agacgagctc gtacttgtcg agctggtagc
     1021 cgaaggcgac cgagatcagg tacagcccgc cgaggacggc gaggtgcacc cgcacgcggg
     1081 tgacgaagac ctcgccgccg cgggtgaccg cgaggaggta gcgcgcaccg acgacgagca
     1141 gcgacgcgag gaggaggccg ttcgccaacg cctggacgaa ccggaagaac ggcagctcga
     1201 acaggaagaa gctcacgtcg cggccgaaga tcggatccgt gacggattgc gtcggatcga
     1261 acgggacgcg gtgcagccac agcatcagcg tctcccaggc accggtcacc gcacccgcga
     1321 tgccgagcgc gaggagcacg gcgaccccgg cgatcaccca ggtgccgatc gggacgaggt
     1381 cggggatctc ctccccctcc caggagaacg tcagctcgcc cgttccctgt cgcccgcccg
     1441 agacgcccag cgacccgaac ggaccggtcg atgccctcga ccgggcgctc cgctcggcct
     1501 ggcgctgtgc ctccgcgagg cgtcccgcca cgactcgcag ctgacccggc cgctcgggat
     1561 cggccggcgg tgccatgcgg ttggcgatga ccatgttgcc gagcagcacc acgagcgcga
     1621 gcaccaacgc gccaatgaac agcccggcct ggacgcccag ccgggtccag aacacgctgt
     1681 cgtagccgac gctgcgatac cagatcgcgt cggtccacag gttgatcccg gcgacgagca
     1741 gcagcaggat cgcgccgatc gcgagaatca gggccgcga
//
LOCUS       DBNHXO010000358         6144 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1888207, whole genome shotgun sequence.
ACCESSION   DBNHXO010000358 DBNHXO010000000
VERSION     DBNHXO010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1888207"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..853)
                     /locus_tag="AC162A_07395"
     CDS             complement(167..853)
                     /locus_tag="AC162A_07395"
                     /inference="COORDINATES: protein motif:HMM:NF024248.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5010704.1"
                     /translation="MSSGSNEQDLTPDPDFRPQDVRLIGDVESLRAISDPTRIRILET
                     MVQRQDPPWSVKELAAEIGVPQTRLYHHVEQLLERDLIRAVERRIVSGIIETRYRVAA
                     LSFQLDRGLLAGDSEAGRQALHDTLAAVFDSARGEVEQAIREGVIDMTAGAPDERRVT
                     LSRGLARLSPARAAEFRTRLQALYEEFGDDADPDDRAYGFVFALYPMPRPSGVEATDT
                     DDEATEGATR"
     gene            1156..1995
                     /locus_tag="AC162A_07400"
     CDS             1156..1995
                     /locus_tag="AC162A_07400"
                     /inference="COORDINATES: protein motif:HMM:NF024874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5010705.1"
                     /translation="MPDRHANVDHGWHAVRAASPAVDGGSTRGRITFLGHSTLLIEVD
                     DLRILTDPVLRDGLGPVRRQVRAILPSLFADLDAVFISHGHIDHFDPPSLRRIPGKPT
                     VIVPRGFGKLAAKAGLGPVEEVEPGDRLTIDRVRFEVMYADHSGRREPLGPSGPAIGC
                     VIAGSRTVYFPGDTDLFPEMAALIGRLDVALLPVWGWGPTIGQGHLNPVSAAAAAAIL
                     RPRLAIPIHWGTFYPAGMRRVAPQAFDEPGQTFASALAQFAPDVPARVLAPGEALDIP
                     QRP"
     gene            2448..2996
                     /locus_tag="AC162A_07405"
     CDS             2448..2996
                     /locus_tag="AC162A_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010706.1"
                     /translation="MQHTTTRRFARIGTLALALGLAVAVAPGLALQAQATSWISYTGK
                     PGGLVGGGVACIQGGSIPEITVFYPQVQSTNGQKQVAMVVHQLQQLVQQPNGSYAWKA
                     ILTVGDSYYTGSAAEFRMQSISMRYVAGTFRVRTAIGWIDASGRQVASAVVQLDHVNS
                     TVIFGAFPNTSEVAASCTYYGR"
     gene            3111..5474
                     /locus_tag="AC162A_07410"
     CDS             3111..5474
                     /locus_tag="AC162A_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR5010707.1"
                     /translation="MPEADGDLDPIIGRDVDLARVRDAIAAHRLVTLTAPGGSGKTRL
                     ARAVLHDVPDGREHAFVDASPLRETDLLASTILTALTPEPAAEAEPVTAIVSLLSGRH
                     LLVVVDNLEQLPGAGAVIARLLEAAPGLSVLATSRVPLGQPGEIEIAVPPLALPAEDT
                     PAGVEASPAGRLFLARARMAGRLVSLDPADAPAIAALVRRLDGIPLAIELAAARTRIM
                     TPTELLARIERLGLRAIDPSGSDERRSMAAIIAWTMSLLDATEREVLAAAAACEGFDL
                     DLLEAVCGGRDVVPALEHLATVALVGNPAVHEGHTRFRLLETVRDHVRATTPDPDALA
                     AAHAWAVSARAEAAWAAIDGGDAGGAAAMELDIDNNRRAIIELTRIDADAALRLWRRM
                     HPAWMNGRLHEAIERFDPIRAGLAAPTPELAKSLSNLAIYVRLARGSAAGRAIRQEAA
                     HVAREVGDPLSLASSLAGLAEDAIIQGDVEEAERIAEELSRFSTDQPDARLHADEARV
                     FVLLTLDGPLGDRAIPAFADMITIARRERRNDMVMAWLGNIAFLHLCRGEYEASLRAA
                     DESIAIARTQNQTYLPIQLAYRVTALAELGRLDEAAAVLSEVIGSDLAKASGPFSADI
                     LRAGAAVALAKDQPLLAARFASGSEHQALAQGDELDGGDRLLLERTMGEARRRSREID
                     VELAIRDGAQANLIDLLQALTDLLSGSPARDALPKDSPTLRHGELTRREVEILTLVGQ
                     GRSDPEIAELLFISPKTASVHVANIKGKLGVETRLEAALRARELGLT"
     gene            complement(5502..5951)
                     /locus_tag="AC162A_07415"
     CDS             complement(5502..5951)
                     /locus_tag="AC162A_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HIR5010708.1"
                     /translation="MRFTTTIQLEGKTATGFQVPPDVVEALGRGKRPPVSVTINGYTY
                     RSTVAAYGEIYMLPLAAEHRDGAGVAAGDEIEVDLQLDEAPREVEVPADLAAALADEP
                     EARAFFEGLSYSNKRWFVLNVEGTKNEETRKRRVAKAVEMLREGRKP"
     gene            6065..>6144
                     /locus_tag="AC162A_07420"
     CDS             6065..>6144
                     /locus_tag="AC162A_07420"
                     /inference="COORDINATES: protein motif:HMM:NF046814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089-like zinc ribbon domain-containing
                     protein"
                     /protein_id="HIR5010709.1"
                     /translation="MPHDVIATCPVCSGELSVTRLRCGDCG"
BASE COUNT          876 a         2210 c         2170 g          888 t
ORIGIN      
        1 gcgatcgccg cggtggagcc ggtcaggtcg aggaccagca ggaacagcgc gaggtaggtc
       61 attccgtcgc cgatgtcgga gatcgactgg gcgaggtaga ggcggcggaa gtcggggatg
      121 cggaggaggt cgcggacgcc gatcgcggcc gcggcgggct tgtcagtcat cgggtcgctc
      181 cttcggtcgc ttcgtcgtcg gtgtcggttg cctcgacgcc actcgggcgc ggcatgggat
      241 acagggcgaa cacgaagccg taggcccggt cgtcgggatc ggcgtcgtcg ccgaactcct
      301 cgtacagcgc ctggagccgc gtgcggaact cggcggctcg tgccggggac agccgagcga
      361 ggccgcgcga cagcgtgacc cggcgctcgt ccggcgcccc ggcggtcatg tcgatgaccc
      421 cctcgcggat cgcctgctcg acctcgcccc gggctgagtc gaacacggca gccagggtgt
      481 cgtggagggc ctgcctgccg gcctcggagt cgccggcgag cagcccgcgg tccagctgga
      541 acgacagcgc ggccacgcgg tatcgggtct cgatgatccc ggacacgatc cgccgctcga
      601 ccgccctgat caggtcacgc tcgagaagct gctcgacgtg gtggtagagc cgggtctggg
      661 gaacgccgat ctccgcggcc agctccttga cggaccacgg cgggtcctgg cgttggacca
      721 tcgtctccag gatccggatg cgcgtcgggt cggagatcgc gcgcaggctt tcgacgtcgc
      781 caatgaggcg gacgtcctgg ggccggaagt ccggatccgg cgtgaggtcc tgctcgttcg
      841 atccgcttga catttgcact ccatcgacca ttcaagatcc gttgaacgat aggatgcacg
      901 ctgaatagcg gttgtcaagg ggtttgactg cgctcggatc acgaggccgc ggcgacacgc
      961 caccgggagt ggcacaaggg agcacggctt cgagatcgtg ctacccggac gcccaaacgg
     1021 gaacgaacct ggttcgaact tcgccacttg tggcgtccgg ctccccgaat ctcgaacctg
     1081 cgtgccgttg tgcgagccgg ggtggcgcgc ggacggacgc aggaggcggg acgcgggatg
     1141 cggcaccatg gacggatgcc ggaccgccac gccaacgtcg accatgggtg gcacgccgtg
     1201 cgcgccgcgt cgccggcggt cgacggcggg tcgacccgcg gccggatcac gttcctcggc
     1261 cactcgacgc tgttgatcga ggtcgacgac ctgcggatcc tcaccgaccc cgtgctgcgc
     1321 gatggcctcg gccccgtccg tcgccaggtc cgggcgatcc tgccgagcct gttcgccgac
     1381 ctcgacgccg tgttcatcag ccacggccac atcgaccact tcgatccgcc gtcgctgcgc
     1441 cggatcccgg gcaagcccac cgtgatcgtc ccgcgcgggt tcgggaagct cgcggccaag
     1501 gctggcctcg gcccggtgga ggaggtcgag cccggcgacc ggctgaccat cgaccgcgtc
     1561 cggttcgagg tcatgtacgc ggatcactcc ggccggcgcg agcccctggg cccgagcggg
     1621 ccggcgatcg gctgcgtgat cgcgggatcg aggacggtct acttccccgg tgacacggac
     1681 ctgttccccg agatggccgc gctcatcggc cggctcgacg tcgcgctgct gccggtgtgg
     1741 ggctggggcc cgacgatcgg ccagggccac ctgaaccccg tgagcgccgc ggccgccgcg
     1801 gcgatcctgc ggccgaggct cgcgatcccg atccactggg gcacgttcta cccggcgggg
     1861 atgcgccgcg tggctccgca ggcgttcgat gagcccggcc agaccttcgc gtcggcgctg
     1921 gcccagttcg ccccggacgt cccggcccgg gtcctcgccc ccggggaggc actcgacatc
     1981 ccccaacgcc cgtaacgcaa cgaaccccgc cgattccggc ggggttcgcg catgtgacga
     2041 gggtcgggct gcttagccga acgcgacgtg ggcgacgatc ccgagcgcgg tgagcagcat
     2101 gaggagcagc agcagcaggg ccgggaagac gccgacggtg tcgacctgga gcggctgggt
     2161 ctgcatgaac atcggtgtct cctttctggt ctgatcgact ggaccgcacg ctcgcggccg
     2221 atgaccacac ggtgccccac gcacacgccg cgacacaccg tcgcgcgccc cgatcgcggc
     2281 gccacgaacg accgctggct cgatggacgg atgacgatgt cccgccgctc aggcgcggaa
     2341 atgaggagcc ccctaaggag gtccccgatg gaggcagcgg cgtcgatcgc gatgctgggc
     2401 accaggtgat cggacgttcg tccgacgact ggacgaggag actcgacatg cagcacacca
     2461 cgactcgacg gttcgctcgc atcggcacgc tggccctggc cctcggcctc gcggtcgccg
     2521 tggccccggg cctcgccctc caggcacagg cgacgtcgtg gatctcctac acgggcaagc
     2581 ccggtggcct ggtcggcggc ggcgtggcct gcatccaggg cggctcgatc cccgagatca
     2641 cggtgttcta cccgcaggtg cagagcacga acggccagaa gcaggtcgcg atggtcgtcc
     2701 accagctcca gcagctcgtc cagcagccga acggcagcta cgcttggaag gccatcctga
     2761 cggtcggcga cagctactac accgggtcgg ccgcggagtt ccggatgcag tccatctcga
     2821 tgcgctacgt cgccgggacg ttccgggtgc gcaccgcgat cggctggatc gatgcctccg
     2881 ggcgccaggt cgcgagcgcg gtcgtccagc tcgaccacgt gaactcgacc gtcatcttcg
     2941 gagcgttccc caacacgtcc gaggtggccg cctcgtgcac ctactacggc cgctgacgtc
     3001 ggccgctgac tacccgttcc accgccgctc gcccggcgcc ccgaggggat gcgccgggcc
     3061 agcggccgtt cgcctccgtc tggcgcacgg cagtccgcta cagtcggccg atgccggagg
     3121 cggacgggga cctcgatcca atcatcggtc gcgacgtgga cctcgcccgg gtgcgcgacg
     3181 cgatcgccgc ccatcggctg gtcacgctga ctgcccccgg cggctcgggc aagactcgcc
     3241 tggcgcgtgc cgtgctccac gacgtgcccg acggccgcga acacgccttc gtcgacgcct
     3301 cgccgctgcg cgagaccgac ctcctggcgt cgaccatcct gaccgcgctg acgccggagc
     3361 cggcggcgga ggccgaaccg gtgacggcga tcgtctccct gctctccggt cgccacctgc
     3421 tcgtggtcgt ggacaacctg gagcagctcc ccggcgcggg cgccgtgatc gcgcgcctcc
     3481 tcgaggcggc ccctgggctg agcgtcctcg cgaccagccg cgtcccgctc ggccagccgg
     3541 gcgagatcga gatcgcggtg ccgccgctgg cgctgcccgc cgaagacacg ccggccggcg
     3601 tcgaggcgtc gcccgcggga cgcctgttcc tggcacgagc gcgcatggcc ggccgcctcg
     3661 tgtcgctcga cccggccgat gcacccgcca tcgccgcgct cgtgcgacgc ctggacggga
     3721 tcccgctggc gatcgagctg gccgcggccc gtacgcggat catgaccccc actgagctcc
     3781 tggcacggat cgaacgactc gggctgcggg cgatcgaccc cagcgggagc gacgagcggc
     3841 gctcgatggc cgcgatcatc gcgtggacga tgagcctgct cgacgccaca gagcgcgagg
     3901 tgctggcggc cgccgcggcg tgtgaggggt tcgacctcga cctcctcgag gccgtgtgcg
     3961 gtggccgcga cgtcgtcccc gcgctcgagc atctcgcgac cgtcgccctg gtcggcaatc
     4021 cggccgtgca cgaagggcac acgcgcttcc gcctgctcga gaccgttcgc gaccacgtgc
     4081 gggccacgac gccggacccc gatgcgctcg ccgcggccca cgcctgggcg gtctcggcac
     4141 gcgccgaggc cgcgtgggcc gccatcgatg gcggcgatgc cggcggcgcg gccgcgatgg
     4201 agctcgacat cgacaacaac cggcgcgcca tcatcgagct gacgcggatc gacgccgatg
     4261 ccgccctgcg gctgtggcgc cgcatgcacc cggcctggat gaacggccgg ctgcacgagg
     4321 cgatcgagcg attcgacccg atccgtgccg gtctcgcggc accgacgccg gagctggcga
     4381 agagcctctc gaatctcgcg atctatgtcc ggctcgcccg aggttcggcc gcaggccgtg
     4441 cgatccggca ggaggcggcg cacgtggcac gggaggtggg cgacccgctg tccctggcct
     4501 cgtccctcgc cggcctcgcc gaggacgcga tcatccaggg cgacgtcgag gaggccgagc
     4561 ggatcgccga ggagttgagc cgtttctcca ccgaccagcc agatgcccgc ctccacgccg
     4621 acgaggctcg cgtgttcgtc ctgctgaccc tcgacggccc gctcggcgat cgggcgatcc
     4681 ctgccttcgc ggacatgatc acgatcgccc ggcgcgagcg acgcaacgac atggtcatgg
     4741 cgtggctcgg caacatcgcg ttcctccacc tgtgtcgcgg agagtacgag gcgagcctcc
     4801 gggcggccga cgaatccatc gccatcgcgc gcacccagaa ccagacctac ctgccgatcc
     4861 agctcgccta ccgggtgacg gcgctcgccg agctcggtcg tctcgacgag gcggcagcgg
     4921 tcctgtccga ggtgatcggc tcggacctcg ccaaggcgtc ggggccgttc agcgcggaca
     4981 tcctgcgggc gggggcggcc gtcgcgctgg cgaaggacca gccgctgctc gcggcgcggt
     5041 tcgcgagcgg gtcggagcac caggccctcg cccaggggga cgagctcgac gggggggatc
     5101 gcctgctgct cgagcggacg atgggcgagg cccggaggcg gtcgcgcgag atcgacgtcg
     5161 agctggcgat ccgcgacggc gcgcaggcca acctcatcga cctcctgcag gcgctcaccg
     5221 acctcctctc ggggtcgccg gcgagggacg cgctgccgaa ggactcgccc accctgcggc
     5281 acggcgagct gacgcggcgc gaggtcgaga tcctgaccct ggtcggccag ggccgcagcg
     5341 acccggagat cgcggagctg ttgttcatca gccccaagac cgcgtcggtc cacgtcgcga
     5401 acatcaaggg caagctcggg gtcgagaccc ggctggaggc ggcgctgcgg gcccgggagc
     5461 tcggtctgac ctgacgcgcc cattgtccgc gtcggccgac gctagggctt gcggccctcg
     5521 cgcagcatct cgaccgcctt ggcgacgcgc cgcttccgtg tctcctcgtt cttcgtgccc
     5581 tcgacgttga gcacgaacca gcgcttgttg ctgtatgaga ggccctcgaa gaaggcgcgc
     5641 gcctcgggct cgtcggccag tgccgccgcc aggtccgccg gcacctcgac ctcacgcggc
     5701 gcctcgtcca gctggaggtc aacctcgatc tcgtcgccgg cggcgacccc ggccccgtcg
     5761 cggtgctcgg ccgccagcgg gagcatgtag atctccccgt acgccgcgac cgtgctccgg
     5821 taggtgtagc cattgatcgt gacgctgacc ggcggacgct tgccgcggcc gagcgcctcc
     5881 acgacatcgg gcggcacctg gaaccccgtg gccgtcttgc cctcgagctg gatcgtcgtc
     5941 gtgaaccgca tggcgctcag tgtaacggcg agccggtctc gcacgaagta ttcgcctgac
     6001 ttgacaatag tgaatccgcg tattagaaaa gagaaagacg cattcatatc tgacgaggaa
     6061 gccagtgccg cacgatgtca tcgccacctg cccggtctgt tcgggcgagc tcagcgtcac
     6121 gcgcctccgg tgcggcgact gtgg
//
LOCUS       DBNHXO010000359         2673 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1688906, whole genome shotgun sequence.
ACCESSION   DBNHXO010000359 DBNHXO010000000
VERSION     DBNHXO010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1688906"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1393
                     /locus_tag="AC162A_07425"
     CDS             149..1393
                     /locus_tag="AC162A_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010710.1"
                     /translation="MRHRARTRRRLIVGGATLLALLVPATTLAAPPAWTRHLGSKAED
                     VAGGIAADGKGITIVGTTGGKITHKVEGGSDAFVRRYDRRGAVLWTRQFGTDAQDIGQ
                     DVAADGGGVTVLGSTDGSFSGSGGTLGIEDVFVRRYDRDGKRLWTRQFGAPEDEDPGA
                     IAAGDGGLFVVGTTAGDLGGSQAGDGDAFLRRYDRDGQPEWTRQFGTDRADTAMAVAV
                     DGGGITVGGGTDGDLQGQNAGPFSDAFVRRYDLAGNVLWTRQWGQEGDDTVLSLAADG
                     TGVTAVGYTHADSMGNKPSQAFIRRFNRNGQLQWSRIFGSPDSEVAWGVAADGDALTV
                     TGYTFGDLDADNKGSFDVFVRRYNRSGTAIWKRQFGTKGADLGLDVAADGKGFTVFGH
                     TNASLGGDAKGELDVFVRRYRR"
     gene            1454..2290
                     /locus_tag="AC162A_07430"
     CDS             1454..2290
                     /locus_tag="AC162A_07430"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HIR5010711.1"
                     /translation="MPHGTVIIIGGAEDKIRDRVILGRFVALAGGRDAQIAVISTASS
                     MGPEAGERYRSIFTELGAAAVRPLHAVSRSQANDENAARVVRDASGVFMTGGNQLRLS
                     STIGGTRLADAISTRFQAGAVVAGTSAGASAVSSHMIAFGASGATPKHRMAQIAAGLG
                     LLPGVIVDQHFQQRNRLGRLLSLIAQNPSLLGLGVDEDTAGVVGPDQIMEVIGRGSIT
                     VVDGAGSETDAWEIRGHRPLMISGVVLHSLPAGYRFDLRRRVRVAAPTLHAIQGGEIA
                     SS"
     gene            2364..>2673
                     /locus_tag="AC162A_07435"
     CDS             2364..>2673
                     /locus_tag="AC162A_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010712.1"
                     /translation="MAQAEPAPRGRTRTATAGKGGNGKAPAGTGPATRRPAADKAVAL
                     GPEPSLRIVETRILRGPNYWAREPVIRQVVDLGVLEEFPSNKIPGFVDALVGMLPTLE
                     D"
BASE COUNT          377 a          963 c          989 g          344 t
ORIGIN      
        1 cggatgacgc cggcccgacg ggccggccgg ctggagacga cggtcgacgc ctggcccgtt
       61 ccggtcaccc tcgggctccc tcctcgtcgc ggaattcttg cacgtcggtg tgagaggatg
      121 gccacccact tgagacggag tcgtgacgat gcgccatcga gccaggaccc gccgccgcct
      181 gatcgttggc ggggccacgc ttctcgccct gctcgtcccc gccacaaccc tggccgcccc
      241 gccggcctgg acccgccacc tgggctcgaa ggccgaggac gtcgcgggcg gcatcgcggc
      301 ggacggcaag gggatcacga tcgtcggcac gacgggcggc aagatcaccc acaaggtcga
      361 gggcggcagc gacgcgttcg tccggcgcta cgaccggcga ggcgcggtcc tgtggacgcg
      421 ccagttcggc accgatgccc aggacatcgg ccaggacgtc gcggcggacg gcggcggcgt
      481 cacggtgctg ggctcgacgg acggctcgtt ctcggggtcc ggcgggacgc tcgggatcga
      541 ggacgtgttc gtccgccgct acgaccgcga cggcaagagg ctctggacgc gccagttcgg
      601 ggcgcccgag gacgaggacc cgggggcgat cgcggccggc gacggggggc tgttcgtggt
      661 cgggacgacc gcaggcgacc tcggcggctc gcaggccggc gacggggacg ccttcctgcg
      721 ccgctacgac cgcgacgggc agccggagtg gacccggcag ttcgggacgg accgggcgga
      781 cacggcgatg gcggtcgccg tcgacggcgg cgggatcacc gtcggcggtg gcaccgatgg
      841 cgacctccag ggccagaacg cgggcccgtt ctcggacgcg ttcgtgcgcc gctatgacct
      901 cgcgggcaac gtgctctgga cccgccagtg gggccaggag ggcgacgaca cggtgctctc
      961 gctcgctgcg gacggcaccg gcgtcaccgc ggtgggctac acgcacgccg actcgatggg
     1021 caacaagccc agccaggcgt tcatccggcg cttcaatcgc aacgggcagc tgcagtggtc
     1081 gcgcatcttc ggctcgcccg actcggaggt cgcctggggc gtggcggcgg acggcgacgc
     1141 actgacggtg accgggtaca cgtttgggga cctcgacgcc gacaacaagg gctcgttcga
     1201 cgtcttcgtc cgtcgctaca accggtcggg caccgcgatc tggaagcgcc agttcgggac
     1261 caagggcgcc gatctcgggc tcgacgtcgc cgcggatggc aagggcttca ccgtgttcgg
     1321 ccacaccaac gcgtccctgg gtggcgacgc gaagggcgag ctcgacgtgt tcgtccggcg
     1381 ctaccgccgc tgacgccggt cacggcctga cgggcccggt cggaccccgt cccacggata
     1441 tactccggcc accatgccgc atgggaccgt catcatcatc gggggcgcgg aggacaagat
     1501 ccgggaccgg gtcatcctcg gccgattcgt ggcgctcgcc ggcggtcgcg acgcgcagat
     1561 cgcggtgatc agcaccgcgt cgtcgatggg ccccgaggcc ggtgagcgct accgttcgat
     1621 cttcaccgag ctcggcgcgg cggccgtccg gccgctccac gcggtcagcc ggtcgcaggc
     1681 caacgacgag aacgcggccc gcgtcgtccg ggacgccagc ggcgtgttca tgaccggcgg
     1741 caaccagctc cggctctcgt ccacgatcgg cggcacgcgc ctcgccgacg cgatctcgac
     1801 gcggttccag gccggcgccg tcgtcgccgg gacctcggcc ggggccagcg ccgtctcctc
     1861 gcacatgatc gcgttcggcg cctcgggcgc cacgcccaag caccgcatgg cgcagatcgc
     1921 cgcgggcctc gggctgctgc ccggcgtgat cgtcgaccag cacttccagc agcggaaccg
     1981 gctcggacgg ctgctgtcgt tgatcgccca gaacccgagc ctgctcgggc tcggcgtgga
     2041 cgaggacacg gccggggtcg ttggaccgga ccagatcatg gaggtcatcg gcaggggatc
     2101 gatcacggtg gtcgacggcg cggggtccga gaccgacgcc tgggagatcc gcggccatcg
     2161 cccgttgatg atcagcggcg tcgtgctcca ctcgctgccc gcgggctacc gcttcgacct
     2221 ccggcgccgc gtccgcgtcg cggccccgac cctccacgcg atccagggcg gcgagatcgc
     2281 ctcgagctga cagggcgccg aggtgcgtgg aacgcgcgcc ctggtccgcg tacactcggg
     2341 agcccagcac gcgaggaggc cacgtggcgc aggcggaacc ggctccccga ggtcgaacgc
     2401 ggacggcgac cgcgggcaag ggtggcaacg gcaaggcacc cgcgggaacc gggcccgcca
     2461 cgcgccgccc ggcggccgac aaggcggtcg ccctcggccc cgagcccagc ttgcgcatcg
     2521 tcgagacccg catcctgcgc ggcccgaact actgggcgcg agagcccgtg atccggcagg
     2581 tcgtcgacct cggcgtcctc gaggagttcc cgagcaacaa gatcccgggg ttcgtcgacg
     2641 ccctcgtggg gatgctgccg acgctcgagg acc
//
LOCUS       DBNHXO010000360         4145 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1888433, whole genome shotgun sequence.
ACCESSION   DBNHXO010000360 DBNHXO010000000
VERSION     DBNHXO010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1888433"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="AC162A_07440"
     CDS             <1..1011
                     /locus_tag="AC162A_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010305761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR5010713.1"
                     /translation="ALAVVDEQHRFGVEQRGALEAKATRGAPHVLLMTATPIPRTLGQ
                     VLYADLDVSTLRTPPAGRLPIRTGIRRPEDLDGTWDRVRAEAAEGRRTFVVVPRIGAA
                     TTTTDGEGDDGDGFAGDDDLPDEDVETAWAAAAEVEYERLTALLAPLRVGLVHGRLKP
                     IDRDAEMARFRDGGLDVLVGTTVVEVGVDVPEATMMVVEGAERFGLAQLHQLRGRVGR
                     GTVASFCVLVSTAAEGTTEHERLKAVASTTDGFALAERDFELRREGDVLGLAQSGLPR
                     LRVATLANPDHRDLAAKARGHAEALLGEDGELPPSEPALDRELTDGWLRNVFAGDPAS
                     AA"
     gene            1011..1592
                     /gene="rsmD"
                     /locus_tag="AC162A_07445"
     CDS             1011..1592
                     /gene="rsmD"
                     /locus_tag="AC162A_07445"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR5010714.1"
                     /translation="MADAGRVIAGTAKGVRLDAPGPGTRPLADRVKQTLFAILEPDLE
                     GAVVADLFAGSGAGGIEALSRGASRAVFVERHAATSRVITANLRRAGLEANARVVTRD
                     ALAWLTEPAGAAVDGPFDIVLIDPPYEDTAALRRSLELVGTHLASGGQVVAKHFWRDA
                     PPAAIGLLASGRERRFGETALTFYRRAQEAGEA"
     gene            1589..2089
                     /gene="coaD"
                     /locus_tag="AC162A_07450"
     CDS             1589..2089
                     /gene="coaD"
                     /locus_tag="AC162A_07450"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR5010715.1"
                     /translation="MNVAVYPGSFDPITNGHLDVIARAARVFDRVVVAVLGNPRKSPL
                     LAADERVAIIGEAVAADPRLAGRVEVRTFDGLTVEFCHEVGAGFLVRGLRAIADFETE
                     LQLAHNNHQLAPDVDTVFFMTSLQHSYISSSLVKEIALFGGDVSAMVPAQAAAGLRRA
                     LPSRQG"
     gene            2221..2712
                     /locus_tag="AC162A_07455"
     CDS             2221..2712
                     /locus_tag="AC162A_07455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit B family protein"
                     /protein_id="HIR5010716.1"
                     /translation="MTRNVIIDRDAALNLIDELRVAVPEEVRAAKRINQEGERIIEKA
                     QEEAERIVARAQEQAAFLINERGLTEAADEESRRIIALADEDALGVRRGADDYAAGVL
                     AGLEADVTRTLRSIERGISMLDDRRAAHTPPVEVDDGYEDVPLAMPAMVEDEEEASAA
                     GRR"
     gene            2709..3305
                     /locus_tag="AC162A_07460"
     CDS             2709..3305
                     /locus_tag="AC162A_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR5010717.1"
                     /translation="MTSSGSRGPHPVDTSGDPLTFALSGLMAEAPGTVRDYAIDSVDV
                     DPGDGLILTEPVAGTMRLSRTNRGLVLDARLTTAIAGECARCLRPVVTAVKVRIDEEV
                     LPSIDLASGMPVPLEEDGDPEAPRLTDHHELELRPLVIEAINLVEPIAPLCEPDCPGL
                     CLECGERLAPGHVHDDVAIDPRLEALRAFRVDGSDDSG"
     gene            3436..3648
                     /gene="rpmF"
                     /locus_tag="AC162A_07465"
     CDS             3436..3648
                     /gene="rpmF"
                     /locus_tag="AC162A_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR5010718.1"
                     /translation="MGVPKRKVAHARQMERRSHLAITLPTLIECPHCHEPKLQHRVCP
                     ACGWYNGRQAVEPKPVGGPAGGEESS"
     gene            3656..>4145
                     /locus_tag="AC162A_07470"
     CDS             3656..>4145
                     /locus_tag="AC162A_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014554.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010719.1"
                     /translation="MSSSEDTAAADAGAQRGVRIAVDAMGGDYGVAEVVPGSLAYARA
                     HPDDTVILVGDEPVVLAQAGLLPANVRVVHAPELIGMDEHPARALVRKKRSSIVVATE
                     LVKSGEADAVVTAGHTGAGMAAGKLILGTLPGIERPALAVQMISAGRPFVLLDIGANP
                     DST"
BASE COUNT          565 a         1547 c         1511 g          522 t
ORIGIN      
        1 gcgctggcgg tggtcgacga gcagcaccgg ttcggcgtcg agcagcgcgg ggcgctcgag
       61 gcgaaggcga cccgcggcgc cccgcacgtg ctgctgatga ccgcgacgcc gatcccgcgc
      121 accctcgggc aggtcctgta cgcggacctc gacgtgtcca cgctccggac cccgcccgcc
      181 ggccgcctgc cgatccggac cgggatccgc cggccggagg acctcgacgg gacctgggac
      241 cgcgtccgtg ccgaggcggc cgagggccgc cggacgttcg tggtcgtgcc gcggatcggg
      301 gccgcgacga cgacgaccga cggcgaaggc gacgacggcg acgggttcgc gggcgacgac
      361 gacctgcccg acgaggatgt cgagaccgcg tgggccgcgg ccgccgaggt ggagtacgag
      421 cgcctcacgg cactgctcgc cccgttgcgg gtcgggctcg tgcacgggcg cctcaagccc
      481 atcgaccgcg acgccgagat ggcccgcttc cgcgacggcg gcctggacgt gctcgtgggg
      541 acgacggtgg tcgaggtggg cgtcgacgtc ccggaggcca cgatgatggt cgtggagggc
      601 gcggagcggt tcgggctcgc acagctgcac cagctccgcg gccgggtggg gcgggggacc
      661 gtcgcctcgt tctgcgtcct ggtcagcacg gccgccgagg gcacgaccga gcacgagcgg
      721 ctcaaggcgg tggcctcgac cacggacggc ttcgcgctcg cggagcgcga cttcgagctc
      781 cggcgcgagg gcgacgtcct gggcctggcg cagagtgggt tgccacggct ccgggtcgcg
      841 accctcgcca accccgacca tcgcgacctc gcggcgaagg cccgcgggca cgccgaagcc
      901 ttgctgggcg aggacggcga gctgcccccg agcgagccgg cgctcgatcg cgagctcacc
      961 gatggctggc tgcgcaacgt gttcgcgggc gacccggcga gcgcggcctg atggccgacg
     1021 ccgggcgggt gatcgccggc accgccaagg gcgtgcgcct ggatgcgccg ggccccggca
     1081 cgcggccgct cgccgaccgg gtcaagcaga ccctgttcgc gattctcgag cccgacctcg
     1141 agggcgccgt ggtcgccgac ctgttcgcgg gcagcggcgc gggcgggatc gaggccctgt
     1201 cgcgcggcgc gtcgcgcgcc gtcttcgtcg agcgccacgc ggcgacgtcc cgcgtgatca
     1261 ccgccaacct gcggcgagcc ggcctcgagg cgaacgcccg cgtcgtcacc cgcgacgcgc
     1321 tcgcctggct cacggagccc gcgggcgccg ccgtcgacgg cccgttcgac atcgtactca
     1381 tcgacccgcc gtacgaggac acggccgccc tgcgccgctc gctcgagctc gtgggcacgc
     1441 acctcgcgtc cggcggccag gtcgtcgcca agcacttctg gcgggacgcc ccgccggccg
     1501 cgatcgggct gctagcatcc gggcgggagc gccgcttcgg ggagacggcg ctcaccttct
     1561 accggcgagc gcaggaggca ggcgaggcgt gaacgtcgcg gtctatccgg gctcgttcga
     1621 cccgatcacc aacgggcatc tcgacgtcat tgcgcgcgcg gcgcgcgtgt tcgaccgggt
     1681 cgtggtcgcc gtcctgggca acccccgcaa gtcgccgctg ctggcggccg acgagcgcgt
     1741 cgcgatcatc ggcgaggccg tcgccgcgga cccgcggctg gccggccggg tcgaggtccg
     1801 gaccttcgac ggcctgacgg tcgagttctg ccacgaggtc ggggccgggt tcctcgtccg
     1861 gggcctgcgg gccatcgcgg acttcgagac cgagctccag ctcgcccaca acaaccacca
     1921 gctcgcgccc gacgtcgaca cggtgttctt catgacctcg ctccagcaca gctacatcag
     1981 ctcgagcctg gtcaaggaga tcgccctgtt cgggggcgac gtctccgcga tggtcccggc
     2041 ccaggcggcg gcggggctgc ggcgcgcgct gccgtcacgc cagggttaat ggggtcgcgc
     2101 gtgccataat ccggggcaac gtcccgccgg gaccgcgcgt gcaccggagg ccgaccgctg
     2161 gacatcatct tcctcgtcga gcggctcgaa tcgctgatcg cgaacggcaa gtcactgccg
     2221 atgacccgca acgtgatcat cgaccgggac gccgccctca acctcatcga cgagctccgc
     2281 gtggccgtgc ccgaagaggt gcgtgccgcc aagcggatca accaggaggg ggagcggatc
     2341 atcgagaagg cccaggagga ggccgagcgg atcgtggccc gtgcgcagga gcaggccgcg
     2401 ttcctcatca acgagcgtgg cctgaccgag gccgccgacg aggagagccg gcggatcatc
     2461 gccctcgccg acgaggacgc cctgggcgtc cggcgtggag ccgacgacta cgccgccggg
     2521 gtgctcgccg ggctcgaggc cgacgtgacg cgcacgctgc gcagcatcga gcgggggatc
     2581 tcgatgctcg acgaccgccg tgccgcccac acgccgccgg tcgaggtcga cgacggctac
     2641 gaggacgtcc cgctggccat gccggcgatg gtcgaagacg aggaggaggc ctcggccgcc
     2701 gggcggcgat gacctcgtcc ggatcccgcg ggccgcaccc cgtcgatacg agcggcgacc
     2761 cgctcacctt cgccctgtcg ggcctcatgg ccgaggcgcc cggcaccgtc cgcgactacg
     2821 cgatcgacag cgtcgacgtc gatcccgggg acggtctcat cctcaccgag ccggtagcgg
     2881 gcacgatgcg gctctcgcgc acgaaccgcg gcctcgtgct ggacgcccgc ctcacgaccg
     2941 ccatcgcggg cgagtgcgcg cgttgcctcc gccccgtcgt gaccgcggtc aaggtccgca
     3001 tcgacgagga ggtcctgccc tcgatcgacc tcgccagcgg catgcccgtc ccgctcgagg
     3061 aggacggcga ccccgaggcg ccgcgcctga ccgatcacca cgagctcgag ctgcgcccgc
     3121 tcgtgatcga ggcgatcaac ctcgtcgagc cgatcgcccc gctgtgcgag cccgactgtc
     3181 cgggcctgtg cctcgagtgc ggcgagcggc tcgcgccggg tcacgtccat gacgacgtgg
     3241 cgatcgatcc tcgactcgag gcgctgcggg ccttccgggt cgacggctcg gacgatagcg
     3301 ggtagactcc ccgctcggcc ccaggacccc gcggtcgctc gttcgcgcgc ccatcgtcca
     3361 cggaccagat cgatcaccag accgcgtcgc gggccccgcg cccgcctgac cgtcctcgaa
     3421 ggagctacag acgacatggg tgtgccgaag cgaaaggtcg ctcacgcgcg gcagatggaa
     3481 cgccggagcc acctcgcgat caccctgccg acgctcatcg agtgtccgca ctgccacgag
     3541 ccgaagctgc agcaccgggt ctgccccgcg tgcggctggt acaacggccg gcaggccgtg
     3601 gagccgaagc ccgtcggcgg accggccggg ggcgaggagt cctcgtaacc gtttcgtgag
     3661 ctcgagcgag gacaccgcgg ccgccgacgc gggcgcccag cgcggcgtcc ggatcgccgt
     3721 cgacgcgatg ggcggtgact atggcgtcgc cgaggtcgtg cccggctccc tcgcctacgc
     3781 gcgggcacac cccgatgaca ccgtcatcct cgtcggcgac gagcccgtcg tcctggcgca
     3841 ggcgggcctg ctccccgcca acgtgcgcgt cgtgcacgcg cccgagctga tcgggatgga
     3901 cgagcacccg gcgcgggccc tggtgcgcaa gaagcgcagc tcgatcgtcg tcgccaccga
     3961 gctcgtgaag tcgggcgagg ccgatgcggt cgtcaccgcc ggtcacacgg gtgcgggaat
     4021 ggccgccggc aagctcatcc tgggcacgct gcccggcatc gagcgccccg ccctggccgt
     4081 ccagatgatc tccgccggcc gcccgttcgt cctgctggac atcggcgcca accccgactc
     4141 caccg
//
LOCUS       DBNHXO010000361         2274 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1096748, whole genome shotgun sequence.
ACCESSION   DBNHXO010000361 DBNHXO010000000
VERSION     DBNHXO010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1096748"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..842
                     /locus_tag="AC162A_07475"
     CDS             123..842
                     /locus_tag="AC162A_07475"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5010720.1"
                     /translation="MHERINAYGQPIGPDLPDWQARPWPPRTPMIGRHCRIEPVDPVR
                     DAADLFEAYAEAPDDRDWTYLATERPATLDGFRTHLEGLAATADPFHHTITDIATGRP
                     VGTAALMRIDPPNGVIEIGWINSTRRLQRTVASTEAMYLFMSRVFDELGYRRYEWKCD
                     SRNAPSRATAARLGFQFEGIFRQAAVAKGRSRDTAWFSILDGEWPALRRAYEAWLRPE
                     NFDASGDQVERLADCIARSRG"
     gene            complement(864..1763)
                     /locus_tag="AC162A_07480"
     CDS             complement(864..1763)
                     /locus_tag="AC162A_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR5010721.1"
                     /translation="MALTLGLNLPFVEGSMDGATPRWADIRAMATAGEEVGFDAVWIS
                     DHLGFGDPMGEWAGAWESWTLLSALAVATDRVRLGTYVSAMPLRNPALFAKMAETLDE
                     VSGGRLVLGLGAAWNEPEFRAFGVPFDHRFDRFEEGLRIIAAMFRGGHAEFDGAYAHA
                     GGAPISPRGPRPAGPPIMIGTTGARMLRLTAELADEWNGGMRSIEELVPILAAVDAAC
                     EAAGRDPLTLPRSAEVLVRTIDDPDDRPAEEHELRGTPAELADAVRQYDDLGIAHLQV
                     QLRPNRADAIEAFVPVMRAYLRG"
     gene            1789..2274
                     /locus_tag="AC162A_07485"
     CDS             1789..2274
                     /locus_tag="AC162A_07485"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5010722.1"
                     /translation="MTTATVGEARRPVIRPATEADAGPIRHLIRVTQLNPRDLDWHRF
                     LVADDGGQIVACAQVRVHGAGSRELASVAVEPAREGEGIGRAISEAAISREPVRPLYL
                     YTESARMTFWHRFAFREIEGDDIPRDMRVSVRAARIAMAALSPFTGRRLRVVVMRRDE
                     P"
BASE COUNT          323 a          843 c          813 g          295 t
ORIGIN      
        1 ccgggcaggc ggccgccgcc ggacgcgagc ggccgttcga accggcccgg gcctgaggcc
       61 acgaatagac cggcgcggga cccgtcgtcg cgcggcgggc ctgtgccggg cacacttcga
      121 gcatgcacga gcgcatcaac gcgtacggcc agccgatcgg ccccgacctc cccgactggc
      181 aggcccggcc gtggccgcct cggacgccga tgatcggtcg ccactgccgt atcgagccgg
      241 tcgacccggt tcgagatgcc gcggacctgt tcgaggcgta cgccgaggcg ccggacgatc
      301 gcgactggac ctacctcgcg accgagcgtc ccgccacgct cgacgggttc cgcacccacc
      361 tcgagggcct ggccgcgacc gccgacccgt tccaccacac gatcacggac atcgccaccg
      421 gccgtcccgt cgggacggcc gcgctgatgc ggatcgaccc accgaacggg gtcatcgaga
      481 tcggctggat caacagcacg cggcgactgc agcggacggt tgcgtcgacg gaggcgatgt
      541 acctgttcat gtcccgcgtg ttcgacgagc tgggctaccg gcggtacgaa tggaagtgcg
      601 attcccggaa cgcgccgtcg cgcgcgacgg cggcccgact cgggttccag ttcgagggga
      661 tctttcggca agcggcggtc gccaagggtc gcagccgtga caccgcgtgg ttctcgatcc
      721 tcgacggcga gtggccggcg ctgcgccggg cctacgaggc ctggctgcgg cccgagaact
      781 tcgacgcgag tggcgatcag gtcgagcggc tcgccgactg catcgctcgc tcccgcggct
      841 gagcggtctc ctcgccgacg gcttcagccc cggaggtagg cccgcatcac cggcacgaac
      901 gcctcgatgg cgtcggcacg gttgggccgc agctggacct ggaggtgcgc gatcccgagg
      961 tcgtcgtatt gccgcacggc gtccgcgagc tcggccggtg tcccgcggag ctcgtgctcc
     1021 tcggccggac gatcgtccgg atcgtcgatc gtccgcacga gcacctccgc cgatcggggg
     1081 agggtcagcg gatcgcggcc ggccgcctcg cacgccgcgt cgacggcggc caggatcggg
     1141 accagctcct cgatcgagcg catcccgccg ttccactcgt cggcgagctc ggccgtgagc
     1201 cggagcatgc gggctccggt cgtcccgatc atgatcggcg gcccggcggg ccgcggaccg
     1261 cgaggcgaga tcggcgcgcc gcccgcgtgc gcataggcgc cgtcgaactc ggcgtgtccc
     1321 ccgcggaaca tcgccgcaat gatccgcagc ccctcctcga accggtcgaa gcgatggtcg
     1381 aacggcacgc cgaaggcgcg gaactcgggc tcgttccagg ccgcgccgag gccgagcacc
     1441 aggcggccgc cgctgacctc gtcgagggtc tcggccatct tcgcgaacag ggccgggttg
     1501 cgcagcggca tcgcggacac gtaggtcccc agccgcacgc gatcggtcgc caccgcgagc
     1561 gccgagagga gcgtccacga ctcccaggcc cctgcccact cccccatcgg atcgccgaag
     1621 cccaggtggt cagagatcca gacggcgtcg aagcccacct cctcgcccgc ggtcgccatc
     1681 gcccggatgt cggcccagcg cggcgtggcg ccgtccatcg agccctcgac gaacggcagg
     1741 ttgaggccca gcgtgagcgc cattgcacac gatgctagcg tccgcaccat gaccaccgcc
     1801 accgttggcg aggcgcgccg gccggtgatc cgcccggcga ccgaggccga cgcggggccg
     1861 atccggcacc tcatccgcgt gacccagctg aacccgcgag acctcgactg gcaccgcttc
     1921 ctggtcgcgg acgacggcgg gcagatcgtc gcctgtgccc aggtccgggt ccacggcgcg
     1981 ggcagccggg agctcgcatc cgtggccgtc gagccggccc gcgaaggcga ggggatcggc
     2041 cgcgcgatca gcgaggcggc gatctcgcgc gagccggtgc gaccgctcta cctgtacacc
     2101 gagtcggccc ggatgacgtt ctggcacagg ttcgcgttcc gggagatcga gggcgacgac
     2161 atcccgcgcg acatgcgggt ctcggtccgg gccgcgcgga tcgccatggc ggcgctgtcg
     2221 ccgttcacgg gacggcgact gcgcgtggtg gtcatgcggc gcgacgagcc ctga
//
LOCUS       DBNHXO010000362         3532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3278446, whole genome shotgun sequence.
ACCESSION   DBNHXO010000362 DBNHXO010000000
VERSION     DBNHXO010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3278446"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="AC162A_07490"
     CDS             complement(<1..845)
                     /locus_tag="AC162A_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008859255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR5010723.1"
                     /translation="MAEPVVRDPEVPVAASSGATATRRRGPRRGGAAGAQVAPKDWDL
                     DGLRAALLAELRRQHPQANLDPVERAFDLAVDAHASQVRASGEPYVTHPIASAQILAD
                     LGIDPVAVTAALLHDVPEDTEYSLADVEDRFGPEVAHLVDGVTKLSKFSTHSHEQQQA
                     ENIRKMFLAMADDIRVVLIKLADRLHNMRTLGALAIEKQQRIARQTMEIYAPLAERLG
                     IWQMKWELEDLAFKALEPEAFRELARQLDTRRKGRESYIERAIEELRPRLADAGIEAE
                     LQGRP"
     gene            complement(903..1619)
                     /locus_tag="AC162A_07495"
     CDS             complement(903..1619)
                     /locus_tag="AC162A_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010724.1"
                     /translation="MRGCLFTLLLGAVAIGLFVVVVFPALVAGALTAGVEAAGLQSES
                     TVVTVTSDPPTDLLGMHADQVRIRASDATFRGMEIANLDVTLHDLRFVDRTARSIDGF
                     LDGMVVPDVGGRELALDSVRLSGGGETVIATTRIAGAQVEGLIADGIEAKLGKRPTSV
                     ALTVPDRLTVRLDGTVRGRLIVSPSGDLQLRITNGPAEGLVVTLLRGGVDVPLRLTEA
                     AVSPAGDLELTGELAVSLLG"
     gene            complement(1624..3282)
                     /locus_tag="AC162A_07500"
     CDS             complement(1624..3282)
                     /locus_tag="AC162A_07500"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR5010725.1"
                     /translation="MSVDPTGRPVADLIAAAEPSGTRALGDLVTRLRAADLVRSARRD
                     NLPVGAAGLADVDVAGVTDDSRGVRPGSLFVAVPGFHVDGHDFVAGAAAAGAAAAIVE
                     RPVPGTPLPQLIVSSARAALARSAAWWYGDPTKTIGTVGITGTDGKTTTSFLAVAALE
                     AAGLSAGLVGTVETKVGAIRERHAAHVTTPGAPELQATIAAMTRAGNQVAVIETTSHA
                     LELDRVLGVAWDAAIFTNLTHEHLELHGSFEAYRAAKLRLFKGLAGGGGDPSNPGKVV
                     GGRPWPKVAIVNRDDPAASWFESAAHEAGATVMTYGTDPSAGVRVTGVEEDARRLRVV
                     YVAPSGAAQLDLRLAGRFNVHNALAVVALGEALGLDPAAVREGLVSVEGVPGRMERID
                     EGQPFAVIVDYAHSPASLQGVLDLLAPVAAAAGGGLVVVFGSAGERDTAKRPAMGRIA
                     AERCRLVVATDEDPRGEDRDAIIDEIVRGAEAAGARRGVDVLAIADRRNAIAAAFERA
                     EPGDVVLLAGKGHESTILYADGPLPWDEATVAREVLAAMGHRGR"
     gene            complement(3279..>3532)
                     /locus_tag="AC162A_07505"
     CDS             complement(3279..>3532)
                     /locus_tag="AC162A_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HIR5010726.1"
                     /translation="RAIELALAERRSEMDLGGVDVAGARREPLPGEPTYGLFEHKRSF
                     GATWVPLVGAHERVTRAWRYAAGRAAARAGRAVERRGGA"
BASE COUNT          450 a         1334 c         1284 g          464 t
ORIGIN      
        1 ttggggcggc cctggagctc ggcctcgatg ccggcatcgg cgaggcgcgg ccgcagctcc
       61 tcgatcgccc gctcgatgta gctctcgcgg cccttgcgcc gggtgtcgag ctgccgggcg
      121 agctcgcgga acgcctcggg ctcgagcgcc ttgaacgcga ggtcctcgag ctcccacttc
      181 atctgccaga tcccgagccg ctccgcgagc ggcgcgtaga tctccatggt ctggcgggcg
      241 atccgctgct gcttctcgat cgccagcgcg ccgagggtgc gcatgttgtg caggcggtcg
      301 gcgagcttga tcaggacgac gcggatgtcg tccgccatcg cgaggaacat cttgcggatg
      361 ttctcggcct gttgctgctc gtggctgtgg gtgctgaact tggacagctt ggtgacgccg
      421 tccacgaggt gggcgacctc ggggccgaag cggtcctcga cgtcggcgag gctgtactcg
      481 gtgtcctccg ggacgtcgtg cagcagcgcg gcggtgacgg cgaccgggtc gatgccgaga
      541 tcggcgagga tctgcgccga cgcgatgggg tgggtgacgt acggttcgcc cgacgcgcgg
      601 acctgggacg cgtgggcatc gacggcgagg tcgaacgcgc gctccacggg atcgaggttg
      661 gcctgcgggt gctggcgcct cagctcggcg aggagggccg cccgcagccc gtcgagatcc
      721 cagtccttgg gtgccacctg cgcaccggcc gctccgcccc ggcgggggcc acgccgcctc
      781 gtcgcggtcg cgcccgacga cgccgcgacg ggcacctcgg gatcgcggac gacgggttca
      841 gccatgcgcg gagtctaccc ctgcctcacg cacgggctcg cgccccggac gttcgtcgcc
      901 gatcatccca gcaggctcac cgccagctcg ccggtcagct cgaggtcgcc cgcggggctg
      961 acggcggcct cggtcagccg gagcgggacg tcgacaccgc cgcgcagcag tgtgacgacc
     1021 aggccctcgg ccggcccgtt cgtgatgcgc agctgcaggt cgcccgaggg cgagacgatc
     1081 agccggcccc tgaccgtacc gtcgaggcgg acggtgagcc ggtccgggac cgtgagcgcc
     1141 accgacgtcg gacgcttgcc gagcttggcc tcgatcccgt cggcgatcag gccctccacc
     1201 tgggcacccg cgatccgggt cgtcgcgatc acggtctcgc caccaccgct gagccggacc
     1261 gagtcgagcg cgagctcgcg gccaccgacg tccggaacga ccatgccgtc caggaacccg
     1321 tcgatcgatc gggcggtccg gtccacgaac cgcaggtcgt gcagcgtcac gtcgaggttg
     1381 gcgatctcca tgccccggaa ggtcgcgtcc gacgcccgga tccgcacctg gtccgcgtgc
     1441 atgcccagca ggtcagttgg tggatcgctg gtcaccgtca cgacggtgga ttcggactgc
     1501 aacccggcgg cctcgacgcc cgccgtcagg gcgcccgcga ccaacgccgg gaacacgacc
     1561 accacgaaca gcccgatcgc gaccgcgccc aggagcagcg tgaacaggca tcctcgcatc
     1621 cgctcagcga ccccggtggc ccatcgccgc gagcacctcg cgcgcgactg tcgcctcgtc
     1681 ccacgggagc gggccgtcgg cgtacaggat cgtcgactcg tggcccttgc ccgcgagcag
     1741 cacgacgtcg cccggctcgg cgcgctcgaa cgcggcagcg atcgcgttcc ggcggtccgc
     1801 gatggcgagc acgtcgaccc cccgccgggc gccggcggcc tcggcgccgc gcacgatctc
     1861 gtcgatgatc gcgtcgcggt cctcgccacg gggatcctcg tcggtcgcga cgacgaggcg
     1921 gcaccgctcg gccgcgatcc ggcccattgc cggccgcttg gccgtgtcgc gctcaccagc
     1981 cgacccgaac acgacgacca gccctccgcc ggcggccgcc gccaccggcg cgagcaggtc
     2041 gagcacgccc tggagcgacg ccggcgagtg ggcgtagtcg acgatcacgg cgaacggctg
     2101 gccctcgtcg atccgctcca tgcgcccggg cacaccctcg accgagacca ggccctcccg
     2161 gacggccgcg ggatcgaggc ccagggcctc gcccagcgcg accaccgcga gcgcgttgtg
     2221 gacgttgaag cggccggcca gccggaggtc cagctgggcc gcccccgacg gcgcgacata
     2281 cacgacgcgg aggcgcctgg cgtcctcctc gacccccgtc acgcgcacgc ccgcggaggg
     2341 atcggtgccg taggtcatca ccgtggcgcc cgcctcgtgc gccgccgact cgaaccagga
     2401 ggcggccggg tcgtccctgt tcacgatcgc gaccttgggc catggccgtc cgccgacgac
     2461 cttgcccggg ttggacgggt cgccgccgcc gccggcgaga cccttgaaca gccgcagctt
     2521 ggcggcccgg tacgcctcga atgagccgtg gagctcgaga tgctcgtggg tgaggttcgt
     2581 gaagatcgcg gcgtcccagg ccacgcccag aacgcgatcg agctcgagcg cgtgcgaggt
     2641 cgtctcgatg acggccacct ggttgccggc gcgggtcatc gccgcgatcg tcgcctggag
     2701 ctccggcgcg ccgggcgtgg tcacgtgggc ggcatgccgc tcgcggatgg cgccgacctt
     2761 ggtctcgacc gtcccgacca ggcccgcgga caaccccgcc gcctcgagcg ccgcgaccgc
     2821 gaggaaggag gtcgtcgtct tgccgtccgt gccggtgatc ccgaccgtcc cgatggtctt
     2881 tgtcgggtcg ccgtaccacc aggcggccga ccgggcgagg gcggcgcgcg ccgaggacac
     2941 gatgagctgg ggcagcggtg tccccgggac cgggcgctcg acgatcgcgg ctgccgcgcc
     3001 cgccgctgcg gcgcccgcga cgaagtcgtg gccgtcgacg tggaacccgg gcacggccac
     3061 gaacaggctc ccgggcctga cgccccggga gtcgtccgtg acgccggcga cgtcgacgtc
     3121 ggccagcccg gcggccccga ccggcaggtt gtcccgccgg gcgctcctga cgaggtcggc
     3181 ggcacgcagt cgggtgacga ggtcgcccag cgcccgtgtc ccggagggct ccgccgcggc
     3241 gatcaggtcg gctacgggcc tgcccgtcgg gtccacgctc acgccccgcc cctccgctcc
     3301 acggcccggc cggcgcgcgc ggcggcgcgt ccggcggcgt agcgccacgc acgcgtgacg
     3361 cgctcgtggg cgccgacgag ggggacccag gtcgcgccga aggaacgctt gtgctcgaac
     3421 aggccgtagg tcggctcgcc gggcagcggc tcgcgtcgcg cgccggcgac gtccaccccg
     3481 cccaggtcca tctcgctccg tcgctcggcg agcgcgagct cgatggcccg cc
//
LOCUS       DBNHXO010000363         2623 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2683690, whole genome shotgun sequence.
ACCESSION   DBNHXO010000363 DBNHXO010000000
VERSION     DBNHXO010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2683690"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..715)
                     /locus_tag="AC162A_07510"
     CDS             complement(137..715)
                     /locus_tag="AC162A_07510"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5010727.1"
                     /translation="MTDELAIRYEIVGKRVALGSVRRDLYPLHVAWLNDPEVAWNVFG
                     APVQRSFEEERDWIEAFVADRGSRLWLIYRIDEQRPIGLAALTAIDVEPGTATFRTLI
                     GEAHDRGRGFGREASELVLRHAFEDLGLHEIRLDVFGYNTAGIRLYGRLGFHEVERRP
                     MQLERDGRRWDVIRMAKSIDDPVDAGPSADGR"
     gene            747..1502
                     /locus_tag="AC162A_07515"
     CDS             747..1502
                     /locus_tag="AC162A_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5010728.1"
                     /translation="MPSEGKRRAALVTGVGTRISIGAGIVARLADDGWNLAISFYRPA
                     GPAKASAANIEPEWIAEDLRRRGATVELLPGDLIDPDEPARLISAAVAALGPLRGLVL
                     SHAESVDSEILDTTVESFDRHMIINARASWQLIAEFARQAQGGGAIVALTSDATNYNL
                     PYGASKGALDRIVKAAARELGHLGISANVVNPGPVNTGWIDDDLEAMLIKRQPTGRLG
                     TPEDVAKLIAFLLSDDGAWTTGQLIHTDGGFSS"
     gene            complement(1536..2024)
                     /locus_tag="AC162A_07520"
     CDS             complement(1536..2024)
                     /locus_tag="AC162A_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5010729.1"
                     /translation="MTTVRLDSVILDRREALIIGALAATATVLAACGSSTGTTTSGAA
                     TPGAPTAGSGSAEPAPAGDSTVTILPGGPFDAVLDPPDVTVSRGDTVTWQNDSGEVHT
                     VTCDPDNPLGLDHALPDGAESFNSGNISPGDTFRHAFEVAGTYVYGCALHEAHAGTIT
                     VS"
     gene            complement(2097..2465)
                     /locus_tag="AC162A_07525"
     CDS             complement(2097..2465)
                     /locus_tag="AC162A_07525"
                     /inference="COORDINATES: protein motif:HMM:NF020397.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HIR5010730.1"
                     /translation="MERAQAVDRYIASQSDERRAAMETLRDTINAAAPEATETIAYGM
                     PALRSHGGQFLVSYDAFKNHYSVFPASEAVLEAGGEDLKPYLAGKGTIQFPADQPIPT
                     ALITRIVKARVEENAAVVRT"
BASE COUNT          406 a          906 c          905 g          406 t
ORIGIN      
        1 gccgccgccg aacgcgagcg ccgtcagctc gtcgcgcagg ggccccggcg agccgaaacc
       61 ccagcgcgtc ggctcgcgcg acatcgatcc gccggtcgtg gagacgtcgg tcatgtgact
      121 ctcgcgatcc tgctcatcat cggccatctg cgctcggccc ggcgtcgacg ggatcatcga
      181 tcgacttcgc catccggatc acgtcccagc ggcgcccgtc gcgctcgagt tgcatcggac
      241 ggcgctcgac ctcatggaag ccgagccgcc cgtatagccg gatccccgcc gtgttgtacc
      301 cgaagacgtc caggcggatc tcgtgcaggc cgaggtcttc gaaggcatgc cgaaggacga
      361 gctcactcgc ttcccggccg aagccccggc cacgatcgtg ggcctcgccg atgagcgtgc
      421 gaaacgtcgc ggtgccgggc tcaacgtcga tcgccgtgag cgcagccagc ccgatcgggc
      481 gctgctcgtc gatccggtag atcaaccaca ggcgcgagcc ccggtcggcg acgaatgcct
      541 cgatccagtc gcgctcctcc tcgaaggagc gctgcacggg cgcgccgaac acgttccacg
      601 cgacctcggg gtcgttgagc cacgcgacgt ggagtgggta caggtctcgg cgcaccgagc
      661 cgagagcaac acgtttgccc acaatctcgt agcggatcgc cagctcgtca gtcatcctgc
      721 ccagcatcgc accgcaggag gatcgagtgc ccagcgaggg gaagcggcgg gcggcgctcg
      781 tcacgggcgt cggcacgcgg atcagcatcg gggcggggat cgtggctcgg ctcgcggacg
      841 acggttggaa cctggcgatc tcgttctacc gaccggcggg cccggccaag gcatcggctg
      901 ccaacatcga gccggagtgg atcgccgagg acctgcggcg acgtggcgcg acggtcgagc
      961 tcctcccggg cgatctgatt gaccccgatg agccggcgcg cctgatctcg gcggccgtcg
     1021 ccgcgctcgg tccgctgcgg ggcctcgtcc tttcgcacgc cgagtcggtc gactcggaga
     1081 tcctcgacac gaccgtcgag tccttcgatc gccacatgat catcaacgcc cgggcgagct
     1141 ggcagctcat cgccgagttc gcgcgccagg cgcagggagg cggggcgatc gtcgcgctca
     1201 cgagcgacgc gacgaactac aacctcccgt acggcgcctc gaagggggcg ctcgaccgga
     1261 tcgtgaaggc cgccgcgcgc gagctcggac acctcggcat cagcgcgaac gtcgtcaatc
     1321 ccgggcctgt caatacgggc tggatcgacg acgacctcga ggcgatgctg atcaagcgcc
     1381 agccgacggg ccggctcggg acgcccgagg acgtcgcgaa gctcatcgcg ttcctgctct
     1441 cggacgacgg cgcctggacg accggccagc tcatccacac ggatggcggc ttctcgtcct
     1501 gagatcccga cgtcgacgcg agctggtcgt cccagtcaac tgaccgtgat cgtcccggca
     1561 tgcgcctcgt ggagcgcgca accgtagacg tacgtgccgg caacctcgaa ggcgtgccgg
     1621 aacgtgtcgc ctggggagat gttgcccgag ttgaacgact ccgcaccgtc gggcagggcg
     1681 tggtccagac cgagcgggtt gtccgggtcg caggtgacgg tgtggacctc gccactgtcg
     1741 ttctgccacg tcacggtatc tccgcgcgat acggtcacat caggcggatc caggaccgcg
     1801 tcgaatgggc caccgggcag gatcgtgacg gtcgagtcgc ccgccggcgc cggttcggcc
     1861 gacccggagc ccgccgtcgg cgccccggga gtcgccgcac ctgacgtagt tgtccctgtc
     1921 gatgatccgc aggccgcgag gacggtcgct gtcgccgcca gagcgccaat gatcagggcc
     1981 tcgcgccggt ccaggatgac cgagtcgagt cgaacggtcg tcacgtcgtc accacgtgca
     2041 tctgcgtcag cagagcctac gccccactcg caaggggaag cgcgcggcgg cgtggtttag
     2101 gtgcggacga ccgccgcgtt ctcctcgacc cgggccttga cgatgcgcgt gatcagcgcc
     2161 gtcgggatcg gctggtcggc ggggaactgg atggtgccct tgcccgccag gtatggcttc
     2221 agatcctcgc cgccggcctc gaggacggcc tcgctggcgg gaaacacgct gtagtgattc
     2281 ttgaacgcgt cgtacgacac gaggaactgg cccccgtggc tgcgcagcgc gggcatgcca
     2341 taggcgatgg tctcggtcgc ctcgggcgcg gcggcgttga tggtgtcgcg aagggtttcc
     2401 atcgcagctc ggcgctcgtc cgactgcgat gcgatgtagc ggtcgaccgc ttgggctcgt
     2461 tccatgaaag ggcgttctcc gttctgtgcg tggaatgatc atcgcacgtc ggtcgccggc
     2521 acccccgcag cggccacgag ccgcgagacc acggcctgcg tcagcaccca acccttgagc
     2581 ggatccttcg gcaggcggat tcgcacctgg ttcgaggcga acg
//
LOCUS       DBNHXO010000364         2262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5463723, whole genome shotgun sequence.
ACCESSION   DBNHXO010000364 DBNHXO010000000
VERSION     DBNHXO010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5463723"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="AC162A_07530"
     CDS             <1..807
                     /locus_tag="AC162A_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020524703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR5010731.1"
                     /translation="STPWVMAQLPAFDDDVWELYAPDDWTQAHDLAKDQPERLHALQR
                     LFLIEAAKHNVLPLDDRRVERFNSDLAGRPLLVRGKRQLLFGGMGRLSENSVIVVKNK
                     SHAVTAKLQVPDGGADGVIIAQGGSFGGWSLYAKDGKPAYCYNLLGLRRFKIYGDDPI
                     PAGEHQVRMEFTYDGGGLGKGGLVRLFVDGAPAGEGRLDNTVPLVFSADETTDLGQDT
                     GTPVTDDFGSSGQAFNGRIDWVEIDIDDAAEDLDHLITPEERLRIAMSRQ"
     gene            868..944
                     /locus_tag="AC162A_07535"
     tRNA            868..944
                     /locus_tag="AC162A_07535"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:902..904,aa:Pro,seq:ggg)
     gene            complement(998..2221)
                     /locus_tag="AC162A_07540"
     CDS             complement(998..2221)
                     /locus_tag="AC162A_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010732.1"
                     /translation="MTPVAKFATAAAAVTVITIAGVNVLQAPGPVGPGTPSAAASPSP
                     SPAASPSVAPSPSPVGAARFGPLGSGRYTWPWEGGEVSFAVEDGWTASADGIARRIDT
                     PGEYSLGPHLPGTLNDVTHIYSDACQSAGRLEPVGPSVNDLVAALENQVGTEVVKTNI
                     KVGRAMGTRVEIRETPGLDRSACRHGAGGPLQIWADAAEASSIALAPGYWMLVYAFDL
                     DGRRMVFAGALGPETTEQAVMQLDDLIYSLDFGPPVAGPVPLLPLPGTTASPAGEYGW
                     EGGPNPGSGAGMHKVQGGNHETALVFRVGPACLAGDGQEAVPVRVAGFDGVSIEPYQP
                     PVPFGSGDGDEITRAHALDVGGRTLCVFLTWNPRTTDAELAAAEHVLDTLRAVPMGED
                     RVRITFMLDTGWDVG"
BASE COUNT          339 a          793 c          799 g          331 t
ORIGIN      
        1 agcacgccct gggtcatggc gcagctgccg gccttcgacg acgacgtctg ggagctgtac
       61 gcccccgacg actggacgca ggcccacgac ctcgcgaagg accagccgga gcggctgcac
      121 gcgctccagc gcctgttcct gatcgaggcc gccaagcaca atgtgctgcc cctcgacgac
      181 cgccgcgtcg agcgcttcaa ctccgacctg gccggccggc cactgctggt tcgcggcaag
      241 cgccagctgc tgttcggcgg catgggccgc ctgtccgaga actcggtcat cgtggtcaag
      301 aacaagtccc acgccgtgac ggcgaagctg caggttcccg acggtggcgc ggacggcgtg
      361 atcatcgccc agggtggctc gttcggcggc tggagcctgt acgcgaagga cggcaagccc
      421 gcctactgct acaacctgct cggcctgcgt cggttcaaga tctacggcga cgacccgatc
      481 ccggccggcg agcaccaggt gcgcatggag ttcacgtacg acggcggtgg cctgggcaag
      541 ggcggcctcg tccgcctgtt cgtggacggc gcgccagctg gcgaaggacg cctcgacaac
      601 acggtgccgc tcgtcttctc ggccgacgag acgaccgacc tgggccagga cacggggacc
      661 ccggtgaccg acgacttcgg gtcgagcggt caggcgttca acgggcgcat cgattgggtg
      721 gagatcgaca tcgacgacgc cgccgaggat ctcgaccact tgatcacgcc cgaggagcgg
      781 ctgcggatcg cgatgtcccg ccagtagccg tcggtcaaat ccggcgctca cgatcgcagc
      841 ccgcgactga ggtagactcg tcggcctcgg ggcgtggcgc agcctggtag cgcacctgaa
      901 tggggttcag gaggtcgagc gttcgaatcg ctccgccccg accactcgtc tgaacactga
      961 acggcccctg agactggctc gggggccgtt cgctgcgtta cccgacgtcc cagcccgtgt
     1021 ccagcatgaa cgtgatccgg acgcgatcct cgcccatcgg cacggctcgg agcgtgtcga
     1081 ggacgtgttc ggcggccgcc agctcggcgt ctgtcgtcct cggattccac gtcaggaaca
     1141 cgcacagggt ccggccgccg acgtccaggg catgggcgcg cgtgatctcg tcgccgtccc
     1201 ccgaaccgaa tggcaccggt ggctggtacg gctcgatgga cacgccatcg aatcccgcga
     1261 cgcgtaccgg aacggcctcc tgcccatcgc ccgcgaggca ggctgggccg accctgaaaa
     1321 ccagcgcggt ctcgtggttc ccaccctgga ccttgtgcat ccctgccccg gaacctgggt
     1381 tgggcccgcc ctcccagccg tactcgccgg ccgggctcgc cgtcgttcct gggaggggaa
     1441 gcaaggggac ggggccggcg accggtgggc cgaagtcgag cgagtagatg aggtcgtcga
     1501 gctgcatgac ggcctgctcg gtcgtctccg ggcccagtgc gccggcgaag accatccgcc
     1561 ggccgtcgag gtcgaaggcg tagaccagca tccagtagcc gggggcgagg gcgatggagc
     1621 tcgcctcggc cgcgtcggcc cagatctgga gcgggccccc ggcgccgtgc cggcacgcgg
     1681 atcgatcgag gcccggcgtc tcgcggatct ccaccctggt gcccatcgcc ctgccgacct
     1741 tgatgttggt cttgaccacc tcggtgccga cctggttctc gagcgcggcc accaggtcgt
     1801 tgaccgacgg gcccaccggt tccagccggc ccgccgactg gcaggcgtcg ctatagatgt
     1861 gggtgacgtc gttgagcgtg ccgggaaggt ggggtccaag gctgtactcg ccgggggtgt
     1921 cgatgcgcct ggcgatgccg tcggcgctcg ccgtccagcc gtcctcgacg gcgaacgaca
     1981 cctcgccgcc ctcccagggc caggtgtacc ggcctgatcc cagcgggccg aagcgcgcgg
     2041 cgccaaccgg cgacggcgag ggtgcgactg acggcgacgc cgcaggggac ggcgacggcg
     2101 aggccgcggc cgagggcgta cccggtccaa ccgggcctgg tgcctggagg acgttgacac
     2161 cggcaatcgt gatgacggtc acggcggcgg cagccgtcgc gaacttggcg actggagtca
     2221 tgtccccgat cctccgcgcc ggccaccagg agcggcgctg tc
//
LOCUS       DBNHXO010000365         4096 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1495965, whole genome shotgun sequence.
ACCESSION   DBNHXO010000365 DBNHXO010000000
VERSION     DBNHXO010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1495965"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="AC162A_07545"
     CDS             complement(<1..413)
                     /locus_tag="AC162A_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIR5010733.1"
                     /translation="MGAQGTRAIEAARRSGIPHQVHEYAHDPRVSLREGGRGYALEAV
                     EALGIDPARVFKTIVVAVDGRLGIAVVPADAEVDLKAVADALGGRRATIAAPADAERA
                     TGYVLGGISPLGTRRPLPTVIDDSAAAWPTIHVSAG"
     gene            552..1823
                     /locus_tag="AC162A_07550"
     CDS             552..1823
                     /locus_tag="AC162A_07550"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006118521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5010734.1"
                     /translation="MRFDELGLSADLLRTVTEEGYTEPTPVQERAIPLVLEGRDVLAA
                     AQTGTGKTAAFTLPILDRLRPQANTSFSPARHPVRCLVLTPTRELAVQVAESVKTYSR
                     HVPLRSAVVYGGVPLDPQIKELRAGIEILVATPGRLLDLVGQRAANLGQVEILVLDEA
                     DRMLDMGFMPDIQRILELLGSRKQTLLFSATFSDDIKRLAGSILRTPVTIEVARHGTA
                     AESVRQLVYPVDRDRKEELLAHLIVEGDWHQVLVFTRTKIAASRLASWLDRHGVAATA
                     IHSDRAQSDRTKALEAFKAGEIRVLVATDVAARGLDIEDLPHVVNFELPWNPEDYVHR
                     IGRTGRAGATGDAVSLVCIDEADLLRGVQRLIQTAIPWQTEPGFVPRRDAEPQPLRAP
                     RGASGGYSSGGGAPRRSGSAPRTTRRRSGVA"
     gene            complement(1848..3242)
                     /locus_tag="AC162A_07555"
     CDS             complement(1848..3242)
                     /locus_tag="AC162A_07555"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR5010735.1"
                     /translation="MAGPAGRRGPLASPVRLGAATVIVVAVLVAGFGLFGGSGRSPAG
                     VVAAATVTAGSPSPAPASVAASPATSSPAPSPATTAEPTQKPTPKPTRSPKPPKPTAS
                     PSAPPAVTGGPGTTAAHHAALQRRLDAWRAKHAVPGVSVAMLWDDGREWLGVSGDANI
                     ADDRRVTPDTGFALASISKTFTAAVVLELIDEGKLLLDERVAPLLPAFDIDRRVTVRM
                     LLDHTSDLNDFFFNPRIDHALQSRPDATWTAKRTWRYVPPGRIRPGKVWSYSNTNYLV
                     LGELVKAVTGRSLAKEVRTRLLDPLDLRTAWYQAVEDPETDLSTGYRLVPTARGARAV
                     PVAPASDIMPFRSVVTAAGGAGSIAATAEDTARWMQAFAGGKVLSPTMQAAMLGDAAY
                     TRALRARITYGLGIQLVTINGRAALGHSGRYLGYRNVVRYLPAEGVTIAVLTNQGVVD
                     PAVVAASLLKIARR"
     gene            3399..>4096
                     /locus_tag="AC162A_07560"
     CDS             3399..>4096
                     /locus_tag="AC162A_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010736.1"
                     /translation="MPNAPTAADHLRDVGLIADGPAIWGRPVRLSAPGVFVIEMSTPA
                     ASAPLDLALIGKWVERVDDLLLDGVRPTSKDVQARLASFWLPDQPVLYIGSTTGSVAG
                     RIAAIARTVPGDAKPAASGVWLHFLRNPGDLRVWWAKTDATEEYEDALLDAFAAGISD
                     ADRATVAKGSAGAAASVLPWAVLRSPSGIRKETGIANPLLAEIKPPEPVGVTRIVEFP
                     AGEADGARDEAKRPR"
BASE COUNT          561 a         1504 c         1484 g          547 t
ORIGIN      
        1 ccggccgaga cgtggatcgt gggccacgcg gccgccgagt cgtcgatgac cgtcggcagc
       61 ggccgcctgg tgcccaacgg gctgatcccg ccgagcacgt accccgtcgc ccgctcggcg
      121 tcggccggcg ccgcgatcgt cgcccgacgc ccacccagcg cgtcggcgac cgccttgagg
      181 tcgacctcgg catcggccgg gacgaccgcg atcccgaggc gcccatcgac ggcgacgacg
      241 atggtcttga acacccgcgc ggggtcgatc cccagcgcct cgacggcctc gagcgcatag
      301 ccgcgaccac cctcgcgaag cgagacgcgc gggtcgtgcg catattcatg cacttgatgc
      361 gggatcccgg agcgccgtgc ggcctcgatc gcgcgcgtcc cctgtgcgcc catggggcca
      421 ttgtcgggta tgctccgcgc gaccggtggt cgccgccgcc cccgcacagc cgggtgggcg
      481 ccgcgccgga tatgagaccg tccatgaagg tcgccgccca cgcgaccgca actcggagaa
      541 cctgaacaac cttgcgcttc gacgaacttg gcctgtccgc cgacctgttg cggaccgtga
      601 ccgaggaggg ctacaccgag ccgacaccgg tgcaggaacg ggccatccca ttggtgctcg
      661 agggcaggga cgtcctcgcc gccgcgcaga ccggcaccgg caagacggcc gcgttcaccc
      721 tgcccatcct cgaccgcctc cggccccagg cgaacacgtc gttctcgccg gcgcggcacc
      781 cggtccggtg cctcgtcctg acgccgacgc gcgagctcgc cgtgcaggtc gccgagagcg
      841 tcaagaccta cagccggcac gtgccgctgc ggtcggccgt ggtctacggc ggcgtcccgc
      901 tcgatcccca gatcaaggag ctgcgggccg gcatcgagat cctcgtcgcc acgcctggtc
      961 gcctgctcga cctcgtcggc cagcgtgccg ccaacctggg ccaggtcgag atcctcgtgc
     1021 tcgacgaggc ggaccggatg ctggacatgg gcttcatgcc cgacatccag cggatcctcg
     1081 agctgctcgg gtcgcgcaag cagacgctgc tgttctcggc gaccttctcg gacgacatca
     1141 agcgcctcgc gggctcgatc ctgcgcaccc cggtcacgat cgaggtcgcg cgccacggca
     1201 cggcggccga gagcgtgcgc cagctcgtct acccggtcga tcgcgatcgc aaggaggagc
     1261 tgctcgccca cctgatcgtc gagggcgact ggcaccaggt gctcgtgttc acccggacca
     1321 agatcgcggc ctcgcggctc gcgtcctggc tcgaccggca cggcgtcgcg gcgaccgcga
     1381 tccactccga ccgcgcgcag tccgaccgga cgaaggcgct cgaggcgttc aaggccggcg
     1441 agatccgggt cctcgtcgcg accgacgtcg ccgcccgcgg cctcgacatc gaggacctgc
     1501 cgcacgtcgt caacttcgag ctcccgtgga accccgagga ctacgtccac cgcatcggcc
     1561 ggaccgggcg tgcgggcgca acgggcgacg cggtcagcct cgtgtgcatc gacgaggcgg
     1621 acctgctccg cggcgtccag cgcctcatcc agaccgcgat cccgtggcag acggagccag
     1681 gcttcgtgcc ccggcgagat gccgagccgc agcccctgcg ggcgccgcgg ggtgccagcg
     1741 gcgggtattc gagcgggggt ggggctccgc gacggagcgg atccgccccg cgaacgacga
     1801 ggcgccgctc gggcgtcgcc tgaccccggg cctccgcggg gccgcgctta gcggcgggcg
     1861 atcttgagca gcgatgcggc cacgacggcc gggtcgacca cgccctggtt ggtcaggacc
     1921 gcgatcgtga cgccctcggc gggcaggtag cggaccacgt tgcgatagcc caggtagcgg
     1981 cccgagtggc cgagcgccgc gcgaccgttg atcgtgacga gctggatccc gaggccgtac
     2041 gtgatccgtg cacggagcgc gcgggtgtat gcggcgtcgc cgagcatcgc cgcctgcatc
     2101 gtcggcgaga gcaccttgcc gccggcgaac gcctgcatcc agcgggcggt gtcctccgcc
     2161 gtcgcggcga tcgagcccgc gccaccggcc gcagtgacga ccgatcggaa cggcatgatg
     2221 tcgctggccg gggcgaccgg gaccgccctc gcgcccctgg cggtcgggac gaggcggtag
     2281 cccgtgctga gatcggtctc cgggtcctcg accgcctggt accacgccgt ccgcaggtcg
     2341 agcgggtcga gcaaccgggt ccggacctcc ttcgccagcg aacggccggt cacggccttc
     2401 accagctccc cgagcacgag gtagttggtg ttcgagtagc tccagacctt gccggggcgg
     2461 atacggccgg gcggcacgta gcgccaggtt cgcttcgcgg tccacgtggc gtcgggtcgg
     2521 gactgcagcg cgtggtcgat cctggggttg aagaagaagt cgttcaggtc gctggtgtgg
     2581 tccaggagca tccggaccgt gacccgtcga tcgatgtcga aggcgggcag cagcggcgcc
     2641 actcgctcgt cgagcaggag cttgccctcg tcgatgagct cgagcacgac cgccgcggtg
     2701 aacgtcttgg agatcgaggc cagcgcgaac ccggtgtcgg gcgtcacacg ccggtcgtca
     2761 gcgatgttgg cgtcgcccga gacgccgagc cactcgcgac cgtcgtccca cagcatcgcg
     2821 acggacaccc cgggcaccgc atgcttcgcg cgccacgcgt cgaggcggcg ctggagcgcc
     2881 gcgtggtggg cggcggtcgt gcccggcccg cccgtcacgg ccggcggcgc ggatgggctg
     2941 gcggtgggct tgggcggctt gggggaccgg gtcggcttcg gcgtcggctt ctgcgtgggc
     3001 tccgcggtgg tggccggcga aggggcaggc gacgacgtgg cgggcgatgc cgccactgag
     3061 gcaggggccg gcgacggcga accggcggtg accgtcgcgg cggcgacgac gccggcggga
     3121 gaccgtcccg atccgccgaa cagcccgaag cccgcgacga ggacggcgac gacaatgacg
     3181 gtcgccgctc cgaggcgtac gggcgaggcg agcggtccgc gacggcccgc ggggccggcc
     3241 atgggcgctt cgttacgcat caggtccgat ggggcggcgc tggacgggtt cgttcggcat
     3301 gattggtgag acgatacgct gacacgccgg agatgcgcct tcgcgggcca ccgacgccgt
     3361 ccggctccgc tcccgctatc cggggaaacc cacccgccat gcccaacgcc cccaccgccg
     3421 ccgaccacct tcgcgacgtc ggcctcatag ccgacggtcc agcgatctgg ggccgtcccg
     3481 tgcgcctgtc cgcgcccggc gtgttcgtga tcgagatgtc gacgcccgcc gccagcgctc
     3541 cgttggatct cgcgctcatc ggcaagtggg tagagcgcgt cgacgacctg ctgctcgacg
     3601 gcgtccgccc gacctccaag gacgtccagg cgcgcctcgc ctcgttctgg ctgcccgacc
     3661 agcccgtgct gtacatcggg agcaccaccg gttcggtggc cgggcggatc gcggccatcg
     3721 ccaggaccgt gcccggcgac gccaagcccg cggcgtccgg cgtgtggctc cacttcctgc
     3781 gtaacccggg cgaccttcgg gtgtggtggg cgaagaccga cgccaccgag gagtacgagg
     3841 acgcgctgct cgacgccttc gcggccggca tctccgacgc cgaccgggcc accgtggcca
     3901 agggctcggc cggcgcggcg gcaagcgtcc tgccgtgggc cgtcctgcgg tccccgtccg
     3961 gtatccgcaa ggagacgggc atcgccaacc cgctgctcgc cgagatcaag ccaccggagc
     4021 cggtgggcgt gacccgcatc gtcgagttcc ccgcgggcga ggcggacggc gcgcgcgacg
     4081 aggcgaagcg gccccg
//
LOCUS       DBNHXO010000366         3545 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2487456, whole genome shotgun sequence.
ACCESSION   DBNHXO010000366 DBNHXO010000000
VERSION     DBNHXO010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2487456"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="AC162A_07565"
     CDS             complement(<1..391)
                     /locus_tag="AC162A_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010737.1"
                     /translation="MRDATNALSAMFEASLADADHRIRVEDLLSAVAAVCGEACIAAA
                     GDFDLEHHSFTPGSPVFSDRVNGILCADASDWGSAATSVFGIIRGGALAQGYATDDFP
                     PIDEPIRLFASALAGDGPTPWGFVPLSV"
     gene            635..886
                     /locus_tag="AC162A_07570"
     CDS             635..886
                     /locus_tag="AC162A_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010738.1"
                     /translation="MDDDRMRGWLLARLELAIAAAVVVVIPILGLAFPGRTWHDPWWA
                     GLSPIVAEPIAVVGLVWMVRIWRGPLRDRVPAWRYRDRR"
     gene            1008..2087
                     /locus_tag="AC162A_07575"
     CDS             1008..2087
                     /locus_tag="AC162A_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitinase"
                     /protein_id="HIR5010739.1"
                     /translation="MPASADPAPSAASPSSAASPSPTEVAPPAATPAPTQPAASPAAS
                     ASGSPPAYVFAPYFQTYTSDSLSDVAQASGVKNFTLAFLEATSKNSCALAWNGDKARP
                     LTAGLYLPEIAALRSAGGDVIPSLGGWSADQGGTEIGDSCDNVHDILEAYKLLVTTYG
                     VSRIDMDIEGRSLQRTAAIDRRNQAIRKLQEWAVAEERPLTISYTLPTSATGLEADAL
                     AVLQSAVATGVRIDVVQPMVFDYYDGETTDMGAAAISALTGLHDQLRVLLPSKTDAEL
                     WAMLGATIMPGIDDYPEKSEVTTLAHAQALRDFAADRGMAALSMWAIQRDNGGCPGKA
                     GSDSCSGIEQAPWAFSEILDTSTAP"
     gene            2161..2679
                     /locus_tag="AC162A_07580"
     CDS             2161..2679
                     /locus_tag="AC162A_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010740.1"
                     /translation="MRRQRRDELGIDNPAAWGEFGVALVGASAALAGLTFVAISINLR
                     EILAGPGLTGRAGEAVTTLLLALVTCALLLVPDQGTVALGLEWVVLGIGFGTILLRTH
                     IRAARTLTIERSLLAGRIALHQSAAILWLVAGIATVNGLPGGLYWVAPAALWTIAVGI
                     TDAWVLLIEILR"
     gene            complement(2713..3144)
                     /locus_tag="AC162A_07585"
     CDS             complement(2713..3144)
                     /locus_tag="AC162A_07585"
                     /inference="COORDINATES: protein motif:HMM:NF016001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HIR5010741.1"
                     /translation="MADWDRESFENAVIADMREHGGVVTSGPLAGHPLLILTTLGAKS
                     GKDRRAILTYTQDAGDYVVAGTAGGSPTTPSWVHNLEAEPHVTIEVANRASGATAAIV
                     DADERDRLWEQHVAALPNFAEYPAKAGRVIPVIRITPAPEA"
     gene            complement(3233..>3545)
                     /locus_tag="AC162A_07590"
     CDS             complement(3233..>3545)
                     /locus_tag="AC162A_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyl cyclase domain-containing protein"
                     /protein_id="HIR5010742.1"
                     /translation="AARQGVECAIAIQRALRDHRDDTGFALSVRVGLHTAEANRRGND
                     YSGMGVHIAARVSALAGAGEIIATSDVLADVGAVATSGARDAEVKGVSAPVHVASIGW
                     S"
BASE COUNT          477 a         1301 c         1233 g          534 t
ORIGIN      
        1 gcacgctgag cggaacgaag ccccacggcg tcggaccgtc gccggcgagc gccgaggcga
       61 acagccggat cggctcgtcg atcgggggga aatcgtcggt cgcgtagccc tgggccagcg
      121 cgcctccccg gatgatcccg aacacgctcg tcgcggccga gccccagtcg gacgcgtcgg
      181 cgcacaggat cccgttgacg cggtcggaga acaccggcga tccgggcgtg aacgaatgat
      241 gttcgaggtc gaagtcgccg gcggccgcga tgcaggcctc gccacagacg gcggcgacgg
      301 cgctgagcag gtcctcgacg cggatgcggt gatcggcgtc ggcgaggctc gcctcgaaca
      361 tggcgctgag ggcgttggtc gcgtcgcgca tcccgtcggg atccgtgggg agcctctccg
      421 gctgtgcccg gtgaggctcc ccgtcgtgct cgctggagcg gcggccgaac aacggcatga
      481 cgctctcccc tccatgcggg acatgtccac cctacgcgca tgcggcggct cggtcacggc
      541 cacggcaata gggaaggttc cgcattccgg caccggttgt cacgttgtgc ggcaggcctt
      601 gcgcggcacc acgcgccggg gcatgctgcg cgcgatggac gacgatcgga tgcgcggctg
      661 gctcctcgcg cgcctcgagc tggcgatcgc tgcggccgtc gtcgtggtca tcccgatcct
      721 gggcctcgca tttccgggca gaacgtggca cgacccatgg tgggctggcc tgtcaccgat
      781 cgtggccgaa ccgatcgcgg tcgtcgggct tgtctggatg gtccggatct ggcgcgggcc
      841 gctgcgcgat cgggtgccgg cctggcgata ccgcgatcgg cgctagcgcg cttcgcaccg
      901 gcgcgctgac cgccggactc gtcgcgacca tgctggccgg ctgctcgccc gcgaccagca
      961 cgttcacgac gtcctccgcg cccgcgccaa ccgtccccgc cgcatccgtg cccgcaagcg
     1021 ccgaccccgc gccatcggcc gcatcgccgt cgtcggccgc atccccgtcc ccgaccgagg
     1081 tcgctccccc tgccgcgacc ccggccccga cccagcccgc cgcgtcgccc gctgcgtcgg
     1141 catcgggctc gccgcccgcc tacgtcttcg ccccctactt ccagacctac acctcggact
     1201 cgctcagcga cgtcgcccag gcgtcgggcg tcaagaactt cacgctcgcg ttcctggagg
     1261 ccacgtcgaa gaactcgtgc gcgctcgcgt ggaacggtga caaggcgcgg ccgctgaccg
     1321 cgggcctgta cctgcccgag atcgctgctc tgcgctcggc gggcggtgac gtcatcccgt
     1381 cattgggcgg ctggagcgcc gatcagggcg gcacggagat cggcgactcg tgcgacaacg
     1441 tccacgacat cctcgaggcg tacaagctgc tcgtcacgac ctacggcgtc agccggatcg
     1501 acatggacat cgagggccgg tcgctccagc ggaccgccgc catcgaccgc cgcaaccagg
     1561 ccattcgcaa gctccaggaa tgggccgtcg ccgaagagcg gccgctcacg atctcgtaca
     1621 ccctgccgac gtcggcgacg gggctcgagg ccgatgccct ggccgtgctc cagagcgcgg
     1681 tcgcgacggg ggtccgcatc gacgtcgtgc agccgatggt gttcgactac tacgacggcg
     1741 agacgaccga catgggcgcc gcggcgatct cggcgctgac cgggctccac gaccagctgc
     1801 gggtgctgct gccctcgaag acggacgccg agctgtgggc gatgctgggc gcgacgatca
     1861 tgccaggcat cgacgactac ccggagaagt ccgaggtcac gacgctcgcc cacgcccagg
     1921 cgctgcgcga cttcgcggcc gatcgcggca tggccgcact gtcgatgtgg gcgatccagc
     1981 gcgacaacgg cggctgcccc ggcaaggcgg gctccgacag ctgctcaggg attgagcagg
     2041 cgccctgggc gttcagcgag atcctcgaca cgtccaccgc gccctagcgg gtcgcgaagc
     2101 ggccggtcgc ctcaagccgt tcggatcggc cctcgtcacg ctgcgccgag aggcacaatc
     2161 gtgcgcagac agcggaggga cgagttgggc atcgacaatc cagcggcatg gggcgagttc
     2221 ggcgtcgcgc tcgttggggc atcggccgcg ctcgcgggcc tgacgttcgt cgcgatctcg
     2281 atcaacctgc gcgagatcct cgcggggccc gggttgaccg gacgcgccgg ggaagcggta
     2341 accacgttgc tgctggccct ggtgacgtgc gccctgctgc tggtcccgga ccagggaacc
     2401 gtcgccctgg gcctcgagtg ggtcgtcctg ggcatcgggt ttggcacgat cctgctccgg
     2461 acgcacatcc gcgcggcgcg gacgctcacg atcgagcgct ccctcctcgc cgggcggatc
     2521 gcgctgcacc agtccgcggc gatcttgtgg ctcgtggccg ggatcgcgac cgtcaacggc
     2581 ctgccgggcg gtctgtactg ggtcgcgcct gccgcactgt ggacgatcgc cgtcgggatc
     2641 accgacgcct gggtcctgct gatcgagatc ctgcgctaga cgaccgcgcg gccgtcctga
     2701 tcgtccgccc ggtcaggcct ccggcgcggg cgtgatccgg atcaccggga tcacccggcc
     2761 tgccttcgcc gggtactccg cgaagttggg cagcgcggcg acgtgctgct cccacaggcg
     2821 gtctcgctca tcggcgtcga cgatcgccgc ggtcgcgccc gacgcccgat tggcgacctc
     2881 gatggtcacg tgcggctcgg cctcgaggtt gtgcacccac gacggggtgg tcggtgatcc
     2941 gccggcggtg cccgcgacca cgtaatcgcc cgcgtcttgc gtgtaggtca ggatcgcccg
     3001 ccggtccttg ccgctcttcg cgccgagcgt cgtgaggatc agcagcgggt gtccggccag
     3061 cggcccgctg gtcaccactc cgccgtgctc ccgcatgtcg gcgatcacgg cgttctcgaa
     3121 cgattctcgg tcccagtcgg ccatcgtggc gtcccccgta tgaaagttgc acacgcaacg
     3181 atgccacgga tcgcccggcg tcgccagtca ggcccgtccg gctgactccg gcctaagacc
     3241 aaccgatcga tgcgacgtgg acgggcgcgc tcacgccctt gacctctgcg tcccgggcac
     3301 cgctcgtcgc gacggcgccg acgtccgcga gcacgtctga cgtcgcgatg atctcgcccg
     3361 cgcccgcaag cgcgctgacg cgtgccgcga tgtgcacgcc catgcccgag tagtcgttgc
     3421 cccgccggtt cgcctccgcg gtgtgcagcc cgacgcgtac cgacagggcg aacccggtgt
     3481 cgtcgcgatg gtcgcgcagg gcccgctgga tcgcgatcgc gcattcgacg ccctgccggg
     3541 cggcc
//
LOCUS       DBNHXO010000367         1833 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5266023, whole genome shotgun sequence.
ACCESSION   DBNHXO010000367 DBNHXO010000000
VERSION     DBNHXO010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5266023"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="AC162A_07595"
     CDS             <1..522
                     /locus_tag="AC162A_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5010743.1"
                     /translation="LLVVGAVLFLLGPAIRRRIPLFANASLVLPVAAMTIGAVLIGLD
                     VTGGQTPMSRTPNPVPNTVTSVTTGYRLYQANCAACHGVDGNGGGPLAGTTPITPPSL
                     KAHLGQHTDGDLFYWITTGLPGGMPAWSTTLSEADRWNLINYLRSINGQGPTMPPPAS
                     DAAARPQPHQPGR"
     gene            563..1255
                     /locus_tag="AC162A_07600"
     CDS             563..1255
                     /locus_tag="AC162A_07600"
                     /inference="COORDINATES: protein motif:HMM:NF016149.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0046688 - response to copper ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HIR5010744.1"
                     /translation="MKRFVTAALLAAALLALGSGATLGHSEYEASDPADGATLTEQPT
                     QISATFSERTKPKQSSLRLKSPNGDIIATGGVPAGGPPTKMVIDEVPALDPGVYTVLW
                     TTVALDGDGLKGSFTFTLEASQSAAPSAEASPSAAPSGSPSAAPSPTPAPTASPTPPS
                     TPAASPSATPVASPSASAGTSGSGPSPTTGTDAGAPAASGSDGSIGLLALALAVVLVV
                     AAGTWLYRRRSV"
     gene            1321..>1833
                     /locus_tag="AC162A_07605"
     CDS             1321..>1833
                     /locus_tag="AC162A_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010745.1"
                     /translation="MAISAIALSLALAACAGGTAPAPSMAGVPTARPATASATRIPSA
                     PAVATDGSQATRVDTPFESARYHYRLVVPAGWTINEVPGSGGLHPDEPGVDTFRDREG
                     HILSIVGEPATDLVGWTSPVDLHLRQEHALKVEAHEALTVDGVPATLKAYHLPIPPSY
                     LIHYLDAAVVN"
BASE COUNT          259 a          721 c          592 g          261 t
ORIGIN      
        1 ctcctggtcg tcggtgccgt cctgttcctg ctcggacccg ccatccggcg gcgtatcccg
       61 ttgttcgcga atgcgtcgct cgtcctgccc gtggcggcga tgacgatcgg cgccgtcctc
      121 atcggccttg acgtgaccgg cggccagacc ccgatgtccc ggacgccgaa cccggtgccg
      181 aatacggtca cctccgtgac gaccggctac cggctctacc aggccaattg cgcggcgtgc
      241 cacggcgtcg acggcaatgg gggcggtccc ctcgcgggca ccaccccgat caccccaccc
      301 tccctgaagg cacacctcgg ccagcacacc gacggcgacc tcttctactg gatcacgacc
      361 ggcctgccgg gcggcatgcc cgcctggtcg accaccctca gcgaagcgga tcgctggaac
      421 ctcatcaact acctgcgctc gatcaatggc cagggcccga cgatgccgcc gccggcatcg
      481 gacgcggccg ctcgcccgca accgcaccaa ccaggccggt gagccggttc ggctcgcccg
      541 cgatcgaaag ggacgccatc ccatgaagcg tttcgtcact gcagctctgc tcgccgccgc
      601 actgctcgcg ctcgggtcgg gtgcgacgct cggccactcg gagtacgagg cctcagaccc
      661 cgctgatggc gccacgctca ccgagcagcc aacccagatc agcgcgacct tcagcgagcg
      721 cacgaagccc aagcagagct cgctgaggct caagagcccg aacggtgaca tcatcgcgac
      781 cggcggcgtt ccggctggcg gtccgccgac caagatggtg atcgacgagg tgcccgcgct
      841 cgatcccggg gtgtacacgg tgctctggac caccgtcgcc ctcgatggcg acgggctgaa
      901 gggaagcttc acattcacgc tcgaagccag ccagtccgcg gccccgagcg ccgaggcctc
      961 gccatcggcc gctccatcag gttcgccgtc ggccgcgcct tcgccgaccc cggctccgac
     1021 ggcatcgccg acccctccct cgaccccggc cgcgtcgccg tcggcgaccc ctgtcgcatc
     1081 gccgagcgcg tccgcgggaa cctcgggctc cgggccgtcc ccaaccactg ggaccgacgc
     1141 cggcgcgccg gccgcatccg ggtcggacgg cagcatcggc ctgttggccc tggccctggc
     1201 ggtcgtcttg gtggtcgccg ccggcacctg gctctaccga cgccgcagcg tctgaaccgc
     1261 gcccggagtg ccatagccgg gttcacggag ccggaccacg gcggggcaac gcatcttgtg
     1321 gtggccatct cggccatcgc gctgtcgctc gcgttggccg cctgtgccgg tggcaccgcg
     1381 ccggcgccgt cgatggccgg ggtcccgacc gcccgtcccg cgacggcgtc ggcgacgcgc
     1441 atccccagcg cgcctgccgt cgcgaccgac ggctcgcagg ccacccgtgt cgacacgccg
     1501 ttcgagtcgg ccagatacca ctaccggctg gtcgtgcccg cgggttggac gatcaacgag
     1561 gtcccgggct cgggcgggct ccatccggac gagccgggcg tggacacgtt ccgcgaccgc
     1621 gagggtcaca tcctgagcat cgtcggcgag ccggcgaccg acctcgtggg ctggacgtcg
     1681 ccggtcgacc tccacctccg tcaggaacac gcgctgaagg tcgaggccca cgaggcgctc
     1741 accgttgacg gggtcccggc gacgttgaag gcctatcacc tgccgatccc gccgtcgtac
     1801 ctcatccact acctcgacgc cgccgtggtg aac
//
LOCUS       DBNHXO010000368         4446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2884310, whole genome shotgun sequence.
ACCESSION   DBNHXO010000368 DBNHXO010000000
VERSION     DBNHXO010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2884310"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /gene="metK"
                     /locus_tag="AC162A_07610"
     CDS             <1..866
                     /gene="metK"
                     /locus_tag="AC162A_07610"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR5010746.1"
                     /translation="DAAPVHELGAGDQGMMFGFACRETPELMPLPIALAHRMARRLAE
                     ARKSGQLRYLRPDGKTQVTVEYERGVPKRVRTVVVSAQHDPDVRPERIRSDIAEMVIL
                     PTIPDELRPVDPVMHVNPTGRFVTGGPMGDAGLTGRKIIVDSYGGMARHGGGAFSGKD
                     PTKVDRSAAYAARWVAKNVVAAGLADRFEIEVAYGIGIARPISLTVESFGTGTIPDDK
                     IQQLVERHFDLRPASIIDALDLRRPIYRQTAAYGHFGREDLDLPWERTDKAADLAADA
                     GLPAPELVAVH"
     gene            complement(905..1777)
                     /locus_tag="AC162A_07615"
     CDS             complement(905..1777)
                     /locus_tag="AC162A_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020285009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5010747.1"
                     /translation="MDRWAGLVEFYFRFHTVVVYAFLYLPILVVVVFAFNGTERAITL
                     WGGFSTKWFGIALEDEVVKTALANSFIVAIPNAILATVFGTMAALGLQRVGRKLKMTF
                     DTLTYMSVIVPEIVIALSTLVLFATGFDFVRSTFGWKVNFGYPTIIAAHVLFNMSLVL
                     LLVRARLSGMDRSLTEASADLFATPWRTFRQITFPQLLPAIVAGFLLSFTFSFDDFVI
                     TTFTSGPGSSTLPLFVFGQVRRGVTPETNAVATMMLAFTLLLLLVGQMILVWQARRQG
                     RRSTAAMAGVIADT"
     gene            complement(1834..2733)
                     /locus_tag="AC162A_07620"
     CDS             complement(1834..2733)
                     /locus_tag="AC162A_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5010748.1"
                     /translation="MAGTATGVEPRAPANRLRNRLLTFGLLLPAGAWYLVMLVLPLVI
                     ILIYSFGVRARNGGYEPAFVFDNYARAISKADPFIESLQMSVMGTIGCLLVGLPLAYF
                     LAMRAGRNKSTLLLLLVVPFWTSFLIRTYAWLIILGPNLGLAGQIESIFGVQDVQILG
                     SSFAVLLGLVYGYLPLMVFPLYVTLERMDRTLIEASKDLGAGRWSTFRQVTLPITLPG
                     LVTGSILVFIPMMGEYVIPELLGVGRTYLMGNALVTDFLEARNWPSGAARAVLLIIVM
                     IVTVAGYLWFVNRGRRTRDVSVL"
     gene            complement(2774..4003)
                     /locus_tag="AC162A_07625"
     CDS             complement(2774..4003)
                     /locus_tag="AC162A_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5010749.1"
                     /translation="MTERDIKDLLTQGPVSRRGFLAAAGLTGLSATIAACTGTGGAAS
                     DAPTAAPTAAPTAVPTAAAASGAVESAAPTVAPAPSYAVEDELIMYNWAEYISDENIK
                     KFKAEYGLKKFQYDVFDNNDVLMAKLQGGASGYDVASPTMNYLPAMIESGFLQKLDMS
                     RIPNMELINPVFRKQWWDPTEEYAVPKDYGTTGILYRSTLLPSVPTSWKEFYDLIKGE
                     ASGKTIFVKSRDDVFIFPLKMLGYSLNSVDPNELEQARQILLEVAPHLYGLDSNQYGQ
                     TMADGTASMALGWTGELGQSLKDTKDKGYVVPTEGTAFWLDTWVILADAPHPEASYAW
                     LNFIHRPEIQGEETNFNLYATPNDAAKAYVKPAILADPAIFPSEEALKNLEGQEDTSD
                     STQRNDIWEEFQSKVGG"
     gene            complement(4043..>4446)
                     /locus_tag="AC162A_07630"
     CDS             complement(4043..>4446)
                     /locus_tag="AC162A_07630"
                     /inference="COORDINATES: protein motif:HMM:NF019998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HIR5010750.1"
                     /translation="LAAHVVSADPGRGTADVETDSGLRIVGSVTDPVAQLGAGDAVTL
                     AIRPEAPVVEAREVASEIASERGMTRVAGRVRQGTYLGDTIEYLIDTELAGSLVVRRQ
                     NVRATAGHRAFGPGEPVTLAWNDEANLVLAT"
BASE COUNT          734 a         1546 c         1493 g          673 t
ORIGIN      
        1 acgacgccgc ccctgtccac gagctgggcg ccggcgacca ggggatgatg ttcggcttcg
       61 cgtgccgcga gaccccggag ctgatgcccc tgccgatcgc gctcgcccat cgcatggcgc
      121 gccgcctggc cgaggcccgc aagtccgggc agcttcgcta cctgcgcccc gacggcaaga
      181 cccaggtcac cgtcgagtac gagcgcggcg tgcccaagcg cgtgcgcacg gtcgtcgtct
      241 ccgcgcagca cgaccccgac gtccgtcccg agcggatccg ctcggacatc gccgagatgg
      301 tgatcctgcc cacgatcccc gacgagctcc gccccgtcga cccggtgatg cacgtcaacc
      361 ccacgggtcg cttcgtgacc ggcgggccga tgggcgacgc gggcctcact ggccgcaaga
      421 tcatcgtcga ctcctacggc ggcatggccc gccacggcgg cggcgcgttc agcggcaagg
      481 atccgacgaa ggtcgaccgc tcggccgcct acgccgcgcg ctgggtcgcc aagaacgtcg
      541 tggccgcggg gctcgcggac cggttcgaga tcgaggtcgc ctacggcatc ggcatcgcgc
      601 gcccgatctc gctcacggtc gagtcattcg ggacaggaac gattcccgac gacaagatcc
      661 agcagctggt cgagcgccac ttcgacctcc gcccggcatc gatcatcgac gccctcgacc
      721 tgcgccggcc catctaccgc cagaccgccg cctacggcca cttcggccgc gaggacctgg
      781 acctgccgtg ggagcgcacc gacaaggccg ccgacctcgc ggccgacgcc ggcttgccgg
      841 ccccggagct cgtcgccgtc cactgacgtc cctcgcggcc cgctgagggc ccggcaaccg
      901 gcgatcaggt gtccgcgatc accccggcca tcgccgccgt cgaccggcgg ccctggcggc
      961 gcgcctgcca gaccaggatc atctgcccga ccagcagcag cagcagcgtg aacgcgagca
     1021 tcatcgtcgc gaccgcgttg gtctcgggcg tcacgccgcg acggacctgg ccgaacacga
     1081 acagcggcag ggtcgacgac ccggggcctg acgtgaatgt cgtgatcacg aagtcgtcga
     1141 agctgaacgt gaacgagagc aggaacccgg ccacgatcgc cggcagcagc tgggggaacg
     1201 tgatctgccg gaaggtgcgc catggggtcg cgaacaggtc ggcgctcgcc tcggtcaggg
     1261 accggtccat ccccgacagc cgcgccctga caagcagcag caccaggctc atgttgaaca
     1321 gcacgtgcgc ggcgatgatc gtcgggtagc cgaagttgac cttccagccg aacgtcgatc
     1381 gcacgaagtc gaacccggtg gcgaacagca cgagcgtcga gagcgcgatg acgatctcgg
     1441 ggacgatcac gctcatatag gtgagcgtgt cgaacgtcat cttcagcttg cgcccgaccc
     1501 gctgcagccc gagggccgcc atcgtcccga agaccgtggc caggatcgcg ttgggaatgg
     1561 cgacgatgaa gctgttggcg agcgcggtct tgacgacctc gtcctccagc gcgatcccga
     1621 accacttggt cgagaagccg ccccacaaag tgatggcccg ctcggtcccg ttgaacgcga
     1681 acacgaccac cacgaggatc ggcaggtaga ggaaggcgta cacgacgacc gtgtggaagc
     1741 ggaagtagaa ctcgacgagg ccggcccatc ggtccacccg ccgcccggac cgtgcccgtc
     1801 ggggcgcctg tccggtggcg atcgcgggtg cgctcacagg acgctcacgt cgcgcgtccg
     1861 gcgaccgcgg ttcacgaacc acaggtaacc cgcgaccgtg acgatcatga cgatgatcaa
     1921 caggaccgcc cgcgccgccc cggacggcca gttgcgcgct tcaaggaagt ccgtgaccag
     1981 cgcgttgccc atgaggtagg tccgcccgac tcccagcagc tccgggatca cgtactcgcc
     2041 catcatcggg atgaacacga ggatgctgcc ggtgacgagc ccgggcagcg tgatgggcaa
     2101 ggtgacctgg cggaacgtgg accaccgccc ggcccccagg tccttggacg cctcgatgag
     2161 cgtgcggtcc atgcgttcga gggtcacgta cagcgggaac accatcagcg gcaggtagcc
     2221 gtagaccaac cccagcagga cggcgaacga gctgcccagg atctggacgt cctggacgcc
     2281 gaagatcgac tcgatctgcc ccgcgaggcc caggtttggc cccaggatga tcagccaggc
     2341 gtaggtccgg atcaggaagc tcgtccagaa cggaaccacc aggagcagca ggagcgtgct
     2401 cttgttgcgg cccgcgcgca tggccaggaa gtacgcgagc ggcagaccca cgagcaggca
     2461 gccgatcgtg cccatgaccg acatctggag gctctcgatg aacgggtccg ccttcgagat
     2521 cgcgcgggcg tagttgtcga acacgaacgc gggctcgtag ccgccgttcc tggcccgcac
     2581 gccgaacgag tagatcagga tgatcacgag cggcagcacg agcatgacga ggtaccaggc
     2641 tcccgcaggc agcaggagcc cgaacgtgag caggcggttt cggagccggt tcgccggcgc
     2701 gcgcggctcc acgcccgtcg cggtcccggc catcaggacg acagcgccgg tgcggccaac
     2761 cgctgaggga gcgctacccg ccgaccttcg actggaactc ctcccagatg tcgttgcgct
     2821 gcgtgctgtc cgacgtgtcc tcctggcctt cgaggttctt gagcgcctct tcggacggga
     2881 agatcgccgg gtcggccagg atcgccggct tgacgtacgc cttggccgcg tcgttcggcg
     2941 tggcgtacag gttgaagttc gtctcctcgc cctggatctc ggggcggtgg atgaagttga
     3001 gccacgcgta cgaggcctcg gggtgcggcg cgtccgcgag gatgacccac gtgtcgagcc
     3061 agaacgcggt gccctccgtg ggcacgacgt agcccttgtc cttcgtgtcc ttcaatgact
     3121 ggcccagctc gcccgtccag ccgagcgcca tcgacgccgt gccgtccgcc atcgtctgac
     3181 cgtactggtt ggagtcgagc ccgtagaggt gcggagcgac ctcgagcagg atctggcgcg
     3241 cctgttcgag ctcgttcgga tccacggaat tcagcgagta gcccagcatc ttgaggggga
     3301 agatgaagac gtcgtcgcgg gacttgacga agatggtctt gcccgaggcc tcgcccttga
     3361 tcaggtcgta gaactccttc cacgacgtgg gcaccgacgg gagaagggtg ctccggtaga
     3421 ggatgccggt cgtcccgtaa tccttgggga cggcgtactc ctcggtgggg tcccaccact
     3481 gcttgcggaa caccgggttg atcagttcca tgttggggat gcgcgacatg tcgagcttct
     3541 ggaggaagcc ggactcgatc atcgccggca ggtagttcat ggtcggtgac gccacgtcat
     3601 acccgctcgc gccgccctgg agcttggcca tcaggacatc gttgttgtcg aagacgtcgt
     3661 actggaactt cttgagcccg tactcggcct tgaacttctt gatgttctcg tcggagatgt
     3721 actccgccca gttgtacatg atcagttcgt cttcgaccgc gtaggacggg gcgggcgcca
     3781 cggtgggagc ggcgctctct acggcaccgg acgcggccgc agcggtcggc acggccgttg
     3841 gcgccgcggt cggcgcggcc gtcggtgcat ccgacgcggc cccgccggtc ccggtgcagg
     3901 cagcgatcgt ggccgacagg cccgtcagcc ccgccgcggc caggaatccg cgccggctga
     3961 ccgggccctg ggtcagcaga tccttgatgt cgcgctccgt caccgatgtc ctcctcgtgt
     4021 catggttcga tgggttcgtg ggtcaggtcg caaggaccag gttggcctcg tcattccatg
     4081 ccagcgtcac cggctcgccc ggaccgaagg cccgatgtcc cgccgtggcg cgaacgttct
     4141 gccgccggac cacgagcgat ccggccagct cggtatcgat caggtactcg atcgtgtcgc
     4201 ccaggtacgt cccctggcgg acccgtcccg cgacccgcgt catgccgcgc tccgacgcga
     4261 tctcgctggc cacctcgcgt gcctcgacga ccggcgcctc gggccggatc gccaacgtga
     4321 cggcgtcgcc cgcgccgagc tgcgcgaccg ggtcggtgac cgagcccacg attcgcagcc
     4381 ccgaatccgt ctcgacgtcg gccgtgccgc ggcccggatc ggcgctcacg acgtgcgccg
     4441 cgagga
//
LOCUS       DBNHXO010000369         2514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4273158, whole genome shotgun sequence.
ACCESSION   DBNHXO010000369 DBNHXO010000000
VERSION     DBNHXO010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4273158"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="AC162A_07635"
     CDS             complement(<1..166)
                     /locus_tag="AC162A_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005238117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5010751.1"
                     /translation="MDDEVFRALADTSRRTLLDQLFERDGQTLGDLEAALPAMTRFGV
                     MKHLRVLEAAG"
     gene            266..751
                     /locus_tag="AC162A_07640"
     CDS             266..751
                     /locus_tag="AC162A_07640"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019521223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5010752.1"
                     /translation="MSLAIETERLTLRLRDERDAAWYRELVAERGEDMPTLEAATARL
                     AQFRDLTLELGIGALAIGRRAEGDVIGYCALVIGRCTLDEPEIAYELLQRFHGHGYAT
                     EAGQALVDAAAATGRQRLWSTVRPWNAASLRVLDKLGFLTHHTATDDRGDVVYLVREL
                     P"
     gene            751..2259
                     /locus_tag="AC162A_07645"
     CDS             751..2259
                     /locus_tag="AC162A_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR5010753.1"
                     /translation="MARDRATSAVVIAGGGPTGLMLAGELALAGVEVTIVERRPNQDL
                     PGSRAGGLQSRTIEVFDQRGIADRFLAEGQIAQVAGFGMIRLDISDFPTRHPYGLGLW
                     QKHIERLLAGWLVELEVPIHYGTEVTGFVQDDAGVDVALSDGRGVRAQYLVGCDGGRS
                     LVRKEAGIEFPGWDPTTSALLAEVEMTEEPELGVRRTPSGTHALGKIEYEVKDGAVVY
                     SPGGTVGVMLTEPQVGTREPTVEDLRDGLIAVYGTDYGIHNVTWITRFTDVTRQASAY
                     RKGRVLLAGDAAHVHSPVGGQGLNIGVQDAVNLGWKLAQVVEGISPDSLLDTYHDERH
                     PVGARVLRNTMAQVALTRADDRTEALRDSVSELLAMDEPRRRFAAMMSGLDVHYDLGE
                     GHPLLGRRMPDLDIVTADGPLRVFALLPDARPVLLNLGTPGRLDITPWAHRVQLIEAR
                     YDGPWELPVLGSVEAPSAVLIRPDGYVAWVGDQTQVGLTDALTTWFGPASST"
     gene            complement(2293..>2514)
                     /locus_tag="AC162A_07650"
     CDS             complement(2293..>2514)
                     /locus_tag="AC162A_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010754.1"
                     /translation="LDEAGSVVILGDLVGSVDGALSFGPPAFTADPDRSRKSLERVAG
                     IGVARLLFSHGPEVSDPNERVVGLLASPA"
BASE COUNT          381 a          865 c          880 g          388 t
ORIGIN      
        1 ggccggccgc ctccaggacc cgcaggtgct tcatgacgcc gaaccgggtc atcgccggca
       61 gggccgcctc caggtctccc agcgtctggc catcacgctc gaagagctgg tccagcagcg
      121 tccgccgact ggtatcggcc agcgccctga acacctcgtc gtccatgccc ggacaatagg
      181 tgactgattg gtcacctgtc aagcgcctga ggcttcgtcc gggtcaacgg ccgtccagta
      241 ccttgattcc atgccgtggc aaccgatgtc gctcgccatc gagaccgagc ggctgaccct
      301 ccggcttcgt gacgagcgcg acgccgcctg gtatcgcgag ctggtcgccg agcgcgggga
      361 ggacatgccg acgctcgaag cggccacggc gcgcctcgcg caattccggg atctcacgct
      421 cgagctcggg atcggcgccc tcgcgatcgg gcgtcgcgcg gagggcgacg tgatcggcta
      481 ctgcgccctc gtcatcgggc gctgcaccct ggacgagccg gagatcgcct acgagctgct
      541 ccagcggttc cacggacacg gctatgcaac ggaagcaggg caggcgcttg tggatgccgc
      601 agcggcgacg ggtcggcagc gcctgtggtc gaccgtgcgg ccgtggaacg cggcgtcact
      661 ccgggtgctc gataagctcg ggttcctcac gcaccacaca gccaccgatg atcgtggcga
      721 cgtggtctac ctggtccgcg agctcccgtg atggcccgcg atcgggccac gagcgcggtg
      781 gtgattgccg gcggcggccc gacggggctg atgctggcgg gcgagctggc cttggccggc
      841 gtggaggtca cgatcgtcga gcgtcgtccc aatcaggatc tgccgggttc gcgcgccggc
      901 ggtctgcagt cgcgcaccat cgaggttttc gaccagcgcg gcatcgccga tcggttcctt
      961 gccgaagggc agatcgccca ggtcgccgga ttcggcatga tccgcctgga tatcagcgac
     1021 ttcccgaccc ggcatccgta cgggctcggg ctgtggcaga agcacatcga gcgcctcctc
     1081 gccggctggc ttgtcgagct ggaggtcccg atccattacg gcaccgaggt gaccgggttc
     1141 gtccaggacg acgccggtgt agatgtcgcc ctgtctgacg gccgaggcgt gcgcgcgcag
     1201 tacctggttg ggtgcgacgg cggacgaagc ctggtccgca aggaagccgg catcgagttc
     1261 cccggctggg atccgacgac gagcgccctg ctcgccgagg tcgagatgac cgaggagccc
     1321 gagcttggcg ttcgccgcac gccgtcggga acgcatgcgc tgggcaagat cgagtacgag
     1381 gtcaaggacg gcgcggtggt ctacagcccc ggcggcacag tcggggtcat gctgaccgag
     1441 ccgcaggtcg gcacccgcga gcccacggtg gaggatcttc gcgacggcct cattgccgtc
     1501 tacgggaccg actacgggat ccacaacgtg acctggatca cccggttcac cgacgtgacc
     1561 cgccaggcct ccgcctatcg caagggccgg gtcctgctgg ccggcgacgc tgcccacgtg
     1621 cactccccgg tcggtgggca gggcctcaac atcggcgtcc aggatgcggt gaacctcggc
     1681 tggaagctcg cccaagtggt cgagggcatc tcgcccgaca gcctgctcga cacgtaccac
     1741 gacgagcggc acccggtcgg tgcccgtgtg ctccggaaca cgatggccca ggtcgcgctc
     1801 acgcgcgccg acgatcgcac cgaggcactg cgcgacagcg tgtcggagct gctggccatg
     1861 gacgagcccc gccggcggtt cgccgcgatg atgtccggcc tggacgtcca ctacgacctg
     1921 ggcgagggcc acccgctgct cggacgccgc atgcccgatc tcgacatcgt gaccgccgac
     1981 ggcccgctcc gggtgttcgc cctgctgccc gatgcccggc cagtgctgct caatctgggt
     2041 acgcccggca ggctcgacat cacgccctgg gcgcatcggg tccagctcat cgaagccagg
     2101 tacgacggtc cgtgggagct tccggtgctg ggctcggtgg aggctccatc ggccgtgttg
     2161 atccggcccg acggctacgt ggcctgggta ggagaccaga cgcaggtggg gctcaccgac
     2221 gcgctgacca cctggttcgg accggcgtct tcgacgtgac gggtcgcgtc tccactgtgc
     2281 ggttcgggca cgctacgccg gcgaggccag gagaccgacg acgcgctcgt tcgggtccga
     2341 aacctctggt ccgtgcgaaa acagcaggcg ggcgactccg attcccgcga ctcgttccag
     2401 cgacttgcgc gaccggtcgg gatcggccgt gaaggcaggc gggccgaagg agagggcacc
     2461 gtcgacgctg ccgacgaggt cgcccaggat cacgactgat ccagcctcat cgag
//
LOCUS       DBNHXO010000370         4134 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4472311, whole genome shotgun sequence.
ACCESSION   DBNHXO010000370 DBNHXO010000000
VERSION     DBNHXO010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4472311"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="AC162A_07655"
     CDS             complement(<1..277)
                     /locus_tag="AC162A_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HIR5010755.1"
                     /translation="MRERTDGEGFATRAIHAGERPDPVTHAHNTPIYATATFAFDTAA
                     EKEDAVDRAMAWDPTAYFYSRTGNPTNRALEEKIASLEGAEDSVVAAS"
     gene            383..1135
                     /locus_tag="AC162A_07660"
     CDS             383..1135
                     /locus_tag="AC162A_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010756.1"
                     /translation="MNPSSWWRSLGPIVADDLARVWIPAGLPGFTGEAKHRRREVLDG
                     RFGADGWRTRHLVRGRIVPPSVAIVEYEEAYRRYLRDRPVLVRFLVTACGNVYDDRVE
                     NVDEPDDYDQPATIQNHYQDISVRRVISELVDDPAWPDVVETEVETIDMIDLGTGATH
                     RAPRARGFGGNHLLQIRDPLSPGYVLNPAVVPIHDPALITTLPDRSEWYHVEGCGHLS
                     VEAFWQMSKVIEVRYDRFLALGEGRAQPLADL"
     gene            1132..2043
                     /locus_tag="AC162A_07665"
     CDS             1132..2043
                     /locus_tag="AC162A_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR5010757.1"
                     /translation="MIRGGAPEPRQLLLGALSLDIYLGRDLVLPGGGVLNMAWRWRGS
                     GVPFRLLSRVGDDRPEVFRAFLDRHDIPSEPGLVAPGRSASIDIVIQPDLQPWMDNFV
                     EGVWATFRLLPDEELLVAASGGLHVVLVEGAIRELRRLGEGGLISGTEVTADFLAFRH
                     YSVARMADTMADVDIGFVGWPGELDDPTVAGIRGVAHDQGKLVVVTFGSRGVGVFDGR
                     RDGGGDLSVPVDAIPVRGTTLGCGDAFIAGFLASWRLTRDVWAAVEAGKRLGAEATAW
                     RRPLPDDAYGKTAAIALAAADAAASAG"
     gene            complement(2032..2250)
                     /locus_tag="AC162A_07670"
     CDS             complement(2032..2250)
                     /locus_tag="AC162A_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR5010758.1"
                     /translation="MPFDIGPSALSIVLVAFAILWIAYRVSAAQKKSRPTARDPRAVL
                     EGRLARGEITRDEFDTAMRALGIGDSPG"
     gene            complement(2250..2522)
                     /locus_tag="AC162A_07675"
     CDS             complement(2250..2522)
                     /locus_tag="AC162A_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010759.1"
                     /translation="MLVFSLVLIILGAALIAIGGNRAYKPYKRYKALQEQDANVARYE
                     QWRGGLRTDSKTGASVAMQMLRRQVQIGGAIVGVGVVCMVIGFILR"
     gene            complement(2527..4023)
                     /gene="gatB"
                     /locus_tag="AC162A_07680"
     CDS             complement(2527..4023)
                     /gene="gatB"
                     /locus_tag="AC162A_07680"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR5010760.1"
                     /translation="MTATAERVAAVLDRYEAVVGLVIHCQLKTVSKMFCGCSTAYDGA
                     APNTHVCPVCLGLPGTLPVINRRAVEFVIATGLAIEGSIPERTQWERKNYFYPDLPKG
                     YQISQYAIPLASDGRLAIETSDGPFTVRIRRAHLEEDTAKLVHGDTADAAGARTTLVD
                     FNRSGAPLMEVVTEADIRTGEQARRYAEELQLLMRAIGASDADMERGQMRVEANVSLR
                     LRGEEPFGTRVEVKNMNSFRAVERAIAFEIERQGRMLDAGEPLSMETRGWDDGRQSTY
                     RMRAKETSDDYRYFPEPDLPPLHVEAAWITRVSAMLPELPAARRARYEALGITHYDAA
                     VIVADPGMTGAFEAISGASATSLPAKEVANFVTGSHARIAKTAGLNERGTAGRSTPEG
                     IATLLGAIVEGRVSRPAGRELLDRHLSEGTLADELLAAAGPGPISDDAALLGHIDAVI
                     AANPKAVEDHRAGKPVAGFFVGQVMKATGGAADAARVTVLVRERLDGG"
BASE COUNT          553 a         1502 c         1448 g          631 t
ORIGIN      
        1 cggacgccgc gacgacgctg tcctcggcgc cctcgagcga ggcgatcttc tcctcgagcg
       61 cccgattcgt cgggttgccg gtccgcgagt agaagtacgc ggtcgggtcc caggccatcg
      121 cccggtcgac ggcgtcctcc ttctcggcgg cggtgtcgaa ggcgaacgtg gcggtcgcgt
      181 agatcggcgt gttgtgggcg tgcgtgacgg ggtcgggtcg ctcgcccgcg tggatcgccc
      241 gcgtcgcgaa gccctcgccg tccgtccgtt cccgcatgcc gcgcgatcct ccgagatgcc
      301 ccgttgccgc cggtcaacgg caagcggcca gtctagggtc tggcggcacc cgccggccgg
      361 tctcagcccc taccctgtgg gcgtgaaccc gtccagctgg tggcgatcgc tggggcccat
      421 cgtggccgac gacctcgcgc gggtctggat cccggccggg ctgccgggct tcacgggcga
      481 ggccaagcac cgccgccggg aggtgctgga cggccgattc ggcgcggatg gctggcgcac
      541 gcggcacctc gtccgggggc ggatcgtgcc gccgtccgtc gcgatcgtcg agtacgagga
      601 ggcctatcgc cgctacctgc gcgaccggcc ggtcctcgtg cgcttcctcg tcaccgcctg
      661 cggcaacgtc tacgacgacc gggtcgagaa cgtcgacgag ccagacgact acgaccagcc
      721 cgcgacgatc cagaaccatt accaggacat ctccgtccgg cgcgtgatca gcgagttggt
      781 cgacgacccc gcctggcccg acgtcgtcga gaccgaggtc gagaccatcg acatgatcga
      841 tctgggcacc ggcgcgaccc atcgggcacc ccgagcccgc ggcttcggcg gcaaccacct
      901 gctccagatc cgcgatcccc tgtcgcccgg ctacgtgctc aacccggcgg tcgtcccgat
      961 ccacgacccg gcgctgatca cgacgcttcc cgatcgcagc gagtggtacc acgtcgaggg
     1021 ctgcgggcat ctctcggtcg aggcgttctg gcagatgagc aaggtgatcg aggtccgcta
     1081 cgaccggttc ctggcgctcg gggagggtcg cgcccagccg ctcgccgacc tgtgatccgc
     1141 gggggcgcgc ccgagccgcg gcagctgctg ctcggcgcgc tgagcctcga catctacctc
     1201 ggccgggacc tcgtgctgcc cggcggcggg gtgctcaaca tggcctggcg ctggcgcggg
     1261 agcggcgtcc cgttccgcct gttgtcgcgg gtcggcgacg accggcctga ggtctttcgc
     1321 gccttcctcg atcgccacga catcccgtcc gagcccggtc tggtcgctcc cggccggtcg
     1381 gcatcgatcg acatcgtcat ccagcccgac ctccagccct ggatggacaa cttcgtcgag
     1441 ggcgtgtggg cgacgttccg gctgcttccc gacgaggagt tgctcgtggc ggcatccggc
     1501 ggcctccacg tcgtgctggt cgagggcgcg atccgcgagc tgcggcggct gggcgagggc
     1561 gggctcatca gcgggaccga ggtcacggcc gacttcctcg ccttccgcca ctactcggtc
     1621 gcgcgcatgg cggacacgat ggccgacgtc gacatcggct tcgtaggctg gccgggcgag
     1681 ctcgacgatc cgacggtcgc cgggatccgc ggggtcgcgc acgaccaggg caagctcgtc
     1741 gtcgtgacct tcgggtcgcg cggcgtgggc gtgttcgacg gacgacggga cggcggcggc
     1801 gacctgtccg tgcccgtgga cgcgatcccg gtgcgcggca ccacgctggg ctgcggggac
     1861 gcgttcatcg ccggctttct cgcgtcgtgg cgcctaacgc gggacgtctg ggcggcggtc
     1921 gaggccggca agcgactcgg cgccgaggcc acggcctggc gccgtcccct ccccgacgac
     1981 gcctacggca agacggcggc gatcgccctc gccgcggccg acgcggcggc gtcagccggg
     2041 tgagtcgccg atcccgagcg cccgcatcgc ggtgtcgaac tcgtcccggg tgatctcgcc
     2101 cctcgcgagg cggccctcca ggacggcgcg cgggtcgcgc gccgtcggcc gcgacttctt
     2161 ctgggcggcg ctcacgcgat aggcgatcca caggatcgcg aacgcgacaa ggacgatcga
     2221 gagcgcgctc ggcccgatgt cgaacggcat cagcgcagga tgaacccgat caccatgcac
     2281 acgaccccga cgccgacgat cgcgccgccg atctggacct ggcgccggag catctgcatg
     2341 gccaccgagg caccggtctt cgagtccgtg cgaagcccgc ctcgccactg ctcgtagcgc
     2401 gcgacattgg cgtcctgctc ctgcagcgcc ttgtatcgct tgtacggttt gtaggcccga
     2461 ttgcctccga tcgcgatcag tgccgcgccc aggatgatca gcaccagcga aaagacaagc
     2521 acgccctcac cccccgtcca ggcgctcgcg gaccaggacg gtcacgcggg ctgcgtccgc
     2581 ggcgccaccg gtcgccttca tgacctgacc gacgaagaac ccggcgacgg gcttgccggc
     2641 ccggtggtcc tcgaccgcct tcggattggc ggcgatgacc gcatcgatat gcccgagcaa
     2701 ggccgcgtca tccgagatcg gtccgggacc ggccgccgcg agcaactcgt cggcgagcgt
     2761 gccttcggag aggtgccgat cgagcagctc ccgaccggcc ggccgcgaga cccgcccctc
     2821 gacgatggcg cccaacagcg tcgcgatgcc ttcaggcgtc gaccggccgg ccgtgccgcg
     2881 ttcgttgagc ccggcagtct tggcgatgcg ggcatgggaa ccggtgacga agttggcgac
     2941 ctccttcgcc ggcaggctcg ttgccgaggc ccccgagatg gcttcgaacg cgccggtcat
     3001 gcccggatcg gcgacgatga ccgccgcgtc gtagtgcgtg attccgagcg cctcgtagcg
     3061 cgcgcgccgc gccgcgggca gctccggcag catcgcgctc acgcgcgtga tccacgccgc
     3121 ctcgacgtgg aggggcggga ggtccggctc cgggaagtag cggtagtcgt ccgaggtctc
     3181 cttggcgcgc atccggtagg tcgactggcg tccgtcgtcc cagccgcgcg tttccatcga
     3241 tagcggctcg ccggcgtcga gcatccggcc ctggcgctcg atctcgaacg cgatcgcccg
     3301 ttcgacggcg cgaaacgagt tcatgttctt gacctcgacc cgggtcccga acggctcctc
     3361 gccccgcagt cgcagcgaca cgttcgcctc gacgcgcatc tggccgcgct ccatgtcggc
     3421 gtcgctggcg ccgatcgcgc gcatcagcag ctggagctcc tccgcgtagc gccgcgcctg
     3481 ctccccggtc cggatgtcgg cctcggtcac gacctccatc agcggcgccc ccgaacggtt
     3541 gaagtcgacg agggtcgtgc gcgcgcccgc ggcgtcagcc gtgtcgccgt ggaccagctt
     3601 ggccgtgtcc tcctcgaggt gggcgcgccg gatccggacc gtgaacggac cgtccgacgt
     3661 ctcgatcgcc aggcgtccgt cggacgcgag cgggatcgcg tactggctga tctggtagcc
     3721 cttggggagg tcgggataga agtagttctt gcgctcccac tgcgtccgct cagggatgct
     3781 gccttcgatc gcgaggccgg tcgcaatcac gaactcgacc gcgcgccggt tgatcacggg
     3841 cagggtcccg ggaaggccca ggcacaccgg gcagacgtgg gtgttggggg cggcgccgtc
     3901 gtacgccgtc gagcacccgc agaacatctt cgagaccgtc ttgagctggc agtggatcac
     3961 gaggccgacg accgcctcgt agcgatccag gaccgccgcg acccgctccg ccgtcgcggt
     4021 catggcgcca ccgagccgac cgtcgccagc agcaggccgt acatcgcgac gaccatccat
     4081 ccgatggcga tcgcgacgtt gcgagcagcc atgcggaccc gggtccggtt gtgc
//
LOCUS       DBNHXO010000371         1657 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3478781, whole genome shotgun sequence.
ACCESSION   DBNHXO010000371 DBNHXO010000000
VERSION     DBNHXO010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3478781"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="AC162A_07685"
     CDS             <1..276
                     /locus_tag="AC162A_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridylate synthase B"
                     /protein_id="HIR5010761.1"
                     /translation="APGEAVAATAAVAETPVAVAAVGAEIVAEEPTPAPTAKAAPRKR
                     TSAKAAADVSAATEAPPETAPVEAEAATAPAKPKRTRKAKVDEEVSS"
     gene            378..614
                     /locus_tag="AC162A_07690"
     CDS             378..614
                     /locus_tag="AC162A_07690"
                     /inference="COORDINATES: protein motif:HMM:NF047356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HIR5010762.1"
                     /translation="MVRAPDGTIAIDSTGRAPGRGAYLCRDASCWDAAARKRALEHAL
                     KASIPADVAAALAAGPDAPITIDTDHQGGTRGQE"
     gene            601..>1657
                     /gene="infB"
                     /locus_tag="AC162A_07695"
     CDS             601..>1657
                     /gene="infB"
                     /locus_tag="AC162A_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR5010763.1"
                     /translation="MARSRSGTRGRRGPRKPQRRTGAFTPGAVSVAEPLERGAIELPG
                     TITVKELAELLAVSPADIIRELIKSGIFATINQLIDRDTASLVASELGFEVAEVDATA
                     AGADEEDGSGPAPEATKEVLFDEDKDEDLVARAPIVTVMGHVDHGKTSLLDAIRSTTV
                     ASGERGGITQHIGASEVTTKDGKHIVFLDTPGHEAFTSMRARGARVTDIAVIVVAADD
                     GVMPQTLEAISHAKAAKVPLVIAMNKIDRAEANPDRVKTELAEAGVVIEEFGGDTPMV
                     PVSAKTRVGIDELLEIILLVADLQELKANPKRDAVGTVIEAELDKGRGPVATLIVQTG
                     TLRVGNVVVVGNTFGKVR"
BASE COUNT          276 a          608 c          587 g          186 t
ORIGIN      
        1 gcaccgggcg aggccgtcgc cgcgaccgcc gccgtcgccg agaccccggt cgccgtcgcc
       61 gccgttggcg ccgagatcgt ggccgaggaa ccgacgcccg cccccaccgc caaggcagcg
      121 ccgaggaagc gcacgagcgc caaggccgcc gccgacgtgt ccgcggccac cgaggcaccc
      181 cccgagaccg cccctgtcga ggccgaggcc gcgaccgcac ccgccaagcc caagcggacc
      241 cggaaggcca aggtcgacga ggaggtgtcg tcgtagcccc ggcggccggt cgaacccggc
      301 ccgcaccggc gcggcggccc ccgacccgaa gctgtgtcgc gtgccgcacc gcgcggggca
      361 aggcggagct ggtcaggatc gttcgggcgc cggacggtac catcgcgatc gactcgacgg
      421 gccgcgcgcc cggtcgaggc gcctacctgt gccgggacgc gtcctgctgg gacgcggcgg
      481 cccgcaagcg ggcgctcgag cacgcactga aagcatccat ccccgcggac gtcgcggccg
      541 ccctcgcggc gggacccgac gcccccatca ccatcgacac tgaccatcaa ggaggaacgc
      601 gtggccagga gtaggagcgg cacccgcggc cgacgcggac cccgaaagcc ccagcgccgg
      661 accggcgcct tcacgcccgg agccgtctcg gtcgccgagc cgcttgagcg tggcgcgatc
      721 gagttgccgg gcacgatcac ggtcaaggag ctcgccgagc tgcttgccgt cagccctgcg
      781 gacatcatcc gcgagctgat caagagcggg atcttcgcga cgatcaacca gctcatcgac
      841 cgcgacaccg cgtcgctcgt cgcgagcgag ctcggcttcg aggtggcgga ggttgacgcg
      901 acggccgcgg gcgcagatga ggaggacggc agcggtccgg cccccgaggc gaccaaggag
      961 gttttgttcg acgaggacaa ggacgaggac ctcgtcgcgc gggcgccgat cgtgaccgtc
     1021 atgggccacg tcgaccacgg caagacgagc ctcctcgacg ccatccgttc gacgaccgtc
     1081 gcgtcgggcg agcgtggcgg gatcacgcag cacatcggcg cctccgaggt caccaccaag
     1141 gacggcaagc acatcgtgtt cctggacacc ccgggccacg aggcgttcac gtcgatgcgt
     1201 gcccgtggcg cgcgcgtcac cgacatcgcg gtgatcgtgg tcgctgccga tgacggcgtg
     1261 atgccccaga ccctcgaggc gatcagccac gcgaaggccg ccaaggtccc gctggtcatc
     1321 gcgatgaaca agatcgaccg cgccgaggcc aatccggacc gcgtcaagac cgagctcgcc
     1381 gaggctggcg tggtcatcga ggagttcggc ggcgacacgc cgatggtccc cgtcagcgcc
     1441 aagacccggg tcgggatcga cgagctgctc gagatcatcc tgctggtcgc cgacctccag
     1501 gagctcaagg ccaaccccaa gcgtgacgcg gtcggcaccg tgatcgaggc ggagctcgac
     1561 aagggccgcg gtccggtggc gacgctgatc gtccagaccg gcacgctgcg cgtcggcaac
     1621 gtcgtcgtgg tgggcaacac gttcggcaag gtccgcg
//
LOCUS       DBNHXO010000372         2010 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4473036, whole genome shotgun sequence.
ACCESSION   DBNHXO010000372 DBNHXO010000000
VERSION     DBNHXO010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4473036"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="AC162A_07700"
     CDS             <1..642
                     /locus_tag="AC162A_07700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5010764.1"
                     /translation="LVLEAPLVPRLRAAVDLEGKLVRALDALGPLAGRDAGFVDLPDG
                     GFRDRLTARGIASIPLPLSSPLVIDLPDHSLDALVTLWTAFRGVDDAELREADRVLRP
                     GGRLLVVHNYGRDDVSRLRDAESPEYRVWSRRAGPFLAGDAFKIRVLHCFWTFGSLDD
                     AQSFLGDAFGEQGAALGAELSRPRLSWNVAIYHRWQGGVAPEAVAQRAVAPAQ"
     gene            685..1089
                     /locus_tag="AC162A_07705"
     CDS             685..1089
                     /locus_tag="AC162A_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010765.1"
                     /translation="MSRRYPDGATTRSASARDDPGRSRGPHLGPIRITPVRVTLVIAL
                     VGGIGFLAYATFARDQLQVPMMATGFAICGLVFAAVAALAVFGVIRAGREGRDGRAVL
                     SALVGGLVAVCALMFLSAALIMGMIWSGTRTT"
     gene            1158..1234
                     /locus_tag="AC162A_07710"
     tRNA            1158..1234
                     /locus_tag="AC162A_07710"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1192..1194,aa:Glu,seq:ttc)
     gene            1272..1625
                     /locus_tag="AC162A_07715"
     CDS             1272..1625
                     /locus_tag="AC162A_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010766.1"
                     /translation="MEIMGVTIRTIVTENVATRCDGCREVIDGTPWRVNLLDTVAPEV
                     AVGWTEQTPINPGPHQFHADPACVRRWMAEKGYLLCRRGEVREIMRPVELPLDPPAWG
                     LCDGIHRDDHEFVPA"
     gene            1778..>2010
                     /locus_tag="AC162A_07720"
     CDS             1778..>2010
                     /locus_tag="AC162A_07720"
                     /inference="COORDINATES: protein motif:HMM:NF024139.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR5010767.1"
                     /translation="MPEARSRPERAPAACLPLGQASRLLGVDPDTLRRWADEGRVPAF
                     TTLGGHRRFERRTLERLMASRRTGAVGALGGVG"
BASE COUNT          241 a          732 c          730 g          307 t
ORIGIN      
        1 ttggtgctgg aagcaccgct cgtcccgcgt ctccgggcag cagtcgacct cgagggcaag
       61 ctcgtgcgcg ccctggatgc gctcggcccg ctcgcgggtc gtgacgctgg tttcgtcgac
      121 cttcccgacg gcggcttccg cgatcgcctg accgcgcgcg gcatcgcgag catcccgctc
      181 cccctctcga gccccctcgt gatcgacctg cccgaccaca gcctcgacgc cctcgtcacg
      241 ctctggacgg cgttccgcgg tgtggacgac gcggagctgc gtgaggccga ccgggtgctc
      301 cggcccggcg gccggctcct cgtcgtgcac aactacggcc gcgacgatgt cagcaggctg
      361 cgcgacgccg agtcacccga gtaccgggtg tggagccggc gagcgggccc attcctcgcc
      421 ggtgacgcgt tcaagattcg cgtcctccac tgcttctgga ccttcgggtc gctcgacgac
      481 gcgcagtcgt tcctgggaga cgcgtttggc gaacaggggg cggcgctcgg cgcggagctg
      541 agtcggccgc gcctgtcgtg gaacgtcgcg atctaccacc gctggcaggg tggggtcgcc
      601 cccgaggccg tggcccagcg ggccgtcgcg cccgcccagt agcttccggg agccgggatc
      661 gccggtcctg ctacgctcgc ccggatgagc cgacgctacc cggacggcgc gaccaccagg
      721 agcgcctccg ctcgcgacga tcccggccgg agccgggggc cgcacctcgg tccgatccgg
      781 atcacgcccg tgcgcgtgac gctcgtcatc gcgctggtcg gggggatcgg gttcctggcc
      841 tacgccacct tcgcccgcga tcagctccag gtccccatga tggcgaccgg gttcgcgatc
      901 tgcggcctcg tgttcgccgc ggtggcggcg ctggccgtct tcggggtgat tcgtgccggg
      961 cgggagggtc gcgacgggcg cgcggtgctc tcggcgctgg tcgggggact cgtcgcggtc
     1021 tgcgcgctga tgttcctgtc ggcagcgctg atcatgggca tgatctggag cgggacccgg
     1081 acgacctgag cggacgtcgc gcgacggagc ccgggaaccc ctctgctaca ctcgcccggc
     1141 cccgccgcgg gtcatgggcc cccatcgtct agaggcctag gacgccaccc tttcaaggtg
     1201 gagatcgccg gttcgaatcc ggctgggggt accacctggt ccgccctgcc gccctgctat
     1261 cctcgctcgc gatggagatc atgggcgtga ccatccgcac gatcgtgacg gagaacgtcg
     1321 cgactcgctg cgacgggtgc cgcgaggtca tcgacgggac gccgtggcgc gtcaacctgc
     1381 tggacacggt agcgccagag gtggccgtcg gctggaccga gcagacgccc atcaaccccg
     1441 gcccgcacca gttccacgcc gacccggcgt gcgtccgccg ctggatggcc gagaagggct
     1501 acctgctgtg ccgccggggc gaggtgcgcg agatcatgcg ccccgtggag ctgccgctcg
     1561 acccgcccgc ctggggcctg tgtgacggga tccatcgcga cgaccacgag ttcgtcccgg
     1621 cctgattcgg gggactggcc gggctgcgag tcggccagtt gacgggcgac ggagcgcgtg
     1681 cctacactcc ggcgccgcgg tcgcgtcgag cgaccaaggt ttacccagat tctgacggcc
     1741 tgaccgggca tccgcttgcc ctggagacgg aggactcgtg ccggaagccc ggtcgcgccc
     1801 cgaacgtgcc ccggcggcgt gcctgccgct gggccaggcg agccggctcc tcggcgtcga
     1861 ccccgacacg cttcgtcgat gggccgacga ggggcgcgtc ccggcgttca cgaccctggg
     1921 cggccaccgc cggttcgagc gccggacgct ggagcgcctc atggcgtccc gtcggacggg
     1981 tgccgtgggc gccctcggcg gcgtcggcga
//
LOCUS       DBNHXO010000373         2993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4670991, whole genome shotgun sequence.
ACCESSION   DBNHXO010000373 DBNHXO010000000
VERSION     DBNHXO010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4670991"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1872
                     /locus_tag="AC162A_07725"
     CDS             <1..1872
                     /locus_tag="AC162A_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR5010768.1"
                     /translation="SDRQLLGMLALAGRPLPTGSVDGHERLVRNALAVLTPDGGVAMR
                     HALLADAVVDDLRPDEARALHARLAAIVDDDGERARHLAAAGDQGAAHAVALRAVASS
                     TTPGERAANLGVAAATADGAEANALRIEAAAALRVAGDLTTATRLLDTLERPDNEAAA
                     MAEATRARIEWSAGDPEAMRATIARGLALVAGTDSPAEALLRAESVTVTALVDGRFDE
                     GLDEADGAILLAHRAGADPTRALLLRATILAGLGRPGWADALAAVIESARATGDVETE
                     LSAANNLVSGHEMHGDPASGLRLAASMADRARDLRLSAWERQFGALVANLDLHAGHLE
                     LALRRSENLLDEALDPLAAQQVGLTAGLALVDLGRHEEAVRVLDRLLAVASPDVTGRG
                     DVLFVLAEAALWGGLPDEALTRLEAYREFEGSEYPTSFLVDVTAGWAALEAGRAIPHA
                     CAIGEPTGMLVGARLEREAIEAAAADDDLAASALFDRAGAAYAGFHRRGELRCGWAAA
                     EWRRRAGDPAAVTDLEALEAPLARDGFGPLLVRVHRSLRLLGERRAVRAPATGAARLT
                     AREQEIAKLVARGLTNQEIARRMGLGRPTVARLLSNGMVKLGVDSRGQVAARVGDLV"
     gene            2026..2598
                     /locus_tag="AC162A_07730"
     CDS             2026..2598
                     /locus_tag="AC162A_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010769.1"
                     /translation="MHAALVGLLVLLLAACGGSAGGAATEPADVPTEAAAAASADEPA
                     PTTAAGGGTAGQGWATKPCELFAQADVEQAAGATAMTPAPVPMDATSGLCGYRAADNS
                     AEVVVTVWGGDQATAMSTTIGYLADQGGEGIERVDDLGADAVFSASDGTIFVSKNGTA
                     VQVAIRIPDRDPAAIKAIALDLGRQAASRL"
     gene            complement(2613..>2993)
                     /locus_tag="AC162A_07735"
     CDS             complement(2613..>2993)
                     /locus_tag="AC162A_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5010770.1"
                     /translation="GQPIPDVVMTRTTDLVGSAIDLLQRYGISQLPVTEPAGDGDLAG
                     IVGSVSEKSLLDRAYRNPDVVERTVGEVMDRPLPTLDVGATVDEAFGLLSDGAAALLV
                     IRGGHPAGVVTKLDLLEFLAHSRG"
BASE COUNT          373 a         1098 c         1144 g          378 t
ORIGIN      
        1 tcggatcgac agctgctggg catgctcgcc ctcgccgggc ggccgctacc cacgggctcc
       61 gtggacggcc acgagcgcct cgtacggaat gccctcgcgg tgctcacgcc cgacggcggg
      121 gtggccatgc gccacgcgct gctggccgat gcggtcgtcg acgacctgcg gcccgacgag
      181 gcgagggcgc tccacgcccg gctcgcggcc atcgtggacg acgacggcga gcgagcgcgg
      241 cacctcgcgg cggccggtga ccagggggcc gcccacgccg tcgccctgcg tgcggtcgcg
      301 tcatccacga ctcccgggga acgtgccgcc aacctcggcg tcgccgcggc gaccgcggac
      361 ggagccgagg cgaacgcgct gcgcatcgag gccgccgcgg cgctccgcgt ggccggtgac
      421 ctcaccaccg ccacgaggct gctcgacacg ctcgagcgac ccgacaatga ggcggccgcg
      481 atggccgagg cgacgcgcgc ccggatcgag tggtcggctg gtgaccccga agcgatgcgc
      541 gccaccatcg cgcggggtct cgcgctcgtc gccggcacgg actcgcccgc cgaagcgctc
      601 ctgcgcgcgg agtccgtgac cgtcacggcc ctcgtcgacg gtcgcttcga cgaggggctc
      661 gacgaggccg acggggcgat cctgctcgcg catcgcgccg gcgccgaccc cacccgggcg
      721 ctgctcctcc gcgccacgat cctcgcgggg ctgggccggc ccggctgggc ggacgcgctc
      781 gcggccgtca tcgagtcggc gcgggcgacc ggcgacgtcg agaccgagct gtccgcggcc
      841 aacaacctgg tctcgggcca cgagatgcac ggcgatccgg cgagcggcct ccggctcgcg
      901 gcgtcgatgg ccgaccgcgc gcgagacctc cgcctctcgg cctgggaacg gcagttcggc
      961 gcattggtcg cgaacctcga cctgcacgcc gggcacctcg agctcgccct ccgccgttcg
     1021 gagaacctgc tggatgaggc gctggatccg ttggcggcgc agcaggtcgg cctcacggcc
     1081 ggcctcgcgc tcgtcgacct cggccgtcac gaggaggcgg tccgggtcct cgatcggctg
     1141 cttgccgtcg ccagccccga cgtgacaggg cggggcgacg tgctgttcgt gctcgcggag
     1201 gccgcgctgt ggggcgggct gccggacgag gcgctgacgc ggctggaggc gtaccgcgag
     1261 ttcgaaggct cggagtaccc gacgtcgttc ctggtggacg tgaccgcggg ctgggccgcg
     1321 ctcgaggcgg gccgcgcgat cccccacgcg tgcgcgatcg gcgagccgac cggcatgctc
     1381 gtcggggccc ggctggagcg cgaggcgatc gaagcggcgg cggcggacga cgacctggcc
     1441 gccagcgccc tgttcgaccg ggcgggcgcc gcctacgcgg gcttccaccg gcggggcgag
     1501 ctgcggtgcg ggtgggcggc ggcggagtgg cgccggcggg cgggcgatcc ggcggccgtg
     1561 accgacctgg aggcgctcga ggcaccgctg gcccgggacg gcttcggccc cctgctcgtc
     1621 cgcgtccatc gctcgctgcg cctgctgggt gagcggcggg cggtccgggc gccggcgacc
     1681 ggtgcagccc gactcacggc ccgcgagcag gagatcgcca aactcgtcgc gcggggcctg
     1741 acgaaccagg agatcgcgcg ccgcatgggc ctcgggcgtc cgaccgtcgc ccggctgctg
     1801 tcgaacggca tggtcaagct cggcgtcgac tcgcggggcc aggtcgcggc cagggtcggc
     1861 gacctcgtct gaaggccggc cgcgagcacc gacgtggcgc aggtggacga tgacgcgccg
     1921 ggtcggcgac gggatgctcg ggatcgaggt cgcccgggcg gaccaggccg gcgtctcggt
     1981 cgaggcaaca cctggtcgtc gatcagaggt ccagtgatgc gtctcatgca tgccgccctc
     2041 gtcgggctgc tcgtcctgct cctggccgcc tgtggcggct cggccggtgg cgcggccacc
     2101 gagcccgccg acgtgccaac agaggccgcg gcagcagcca gcgcggacga gcccgcgccg
     2161 acgacagctg cgggcggtgg taccgccggg caaggctggg cgacaaagcc gtgcgagctg
     2221 ttcgcccagg ccgacgtgga gcaggctgcg ggcgccacgg cgatgacccc tgcgccggtc
     2281 ccgatggacg cgacgtccgg tctctgcggc tatcgtgccg ccgacaacag cgcggaggtc
     2341 gtggtcacgg tttggggcgg cgatcaggcc accgcgatgt cgaccaccat cggctatctc
     2401 gccgaccagg ggggcgaggg tatcgagcgg gtcgacgacc tcggcgccga cgcggtcttc
     2461 tcggcgtcgg atggcaccat cttcgtctcg aagaacggca cggccgtgca ggtcgccatc
     2521 cggatccccg accgcgatcc ggcggcgatc aaggccatcg cgctcgacct gggccggcag
     2581 gcggcgagcc gcctctaggt cgtgccggcc ggtcagccgc ggctatgggc gaggaactcg
     2641 agcaggtcga gcttggtcac gacgcccgcc gggtgtccac cacggatgac gagcagcgcc
     2701 gctgccccat ccgagagcag gccgaacgcc tcgtccacgg tcgcgccgac gtcgagcgtc
     2761 ggcagcggcc ggtccatcac ctcgcccacg gtccgctcca cgacgtcggg gttgcgatac
     2821 gcacggtcga gcaggctctt ctcgctcacc gagccgacga tcccggccag gtcgccgtcg
     2881 cccgcaggct ctgtgaccgg gagctggctg atgccgtacc gctggagcag gtcgatcgcc
     2941 gacccgacca ggtcggtcgt ccgtgtcatg accacgtccg ggatcggttg gcc
//
LOCUS       DBNHXO010000374         3464 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1694272, whole genome shotgun sequence.
ACCESSION   DBNHXO010000374 DBNHXO010000000
VERSION     DBNHXO010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1694272"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="AC162A_07740"
     CDS             <1..717
                     /locus_tag="AC162A_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013844.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HIR5010771.1"
                     /translation="RKARLERDESVRTARAEAEKLVDTLRDDVAGIRKRLERETVTAP
                     VIDDAVAHAERTLERLPAREPEAEPTERPEPAEPHVWRLGDRARSRSGGWEGRIAALE
                     KGGTRATLEAGGMRVSVNVDDLEPAVGVGGGTDGDRASARSGGASGVGALQLSKARTV
                     ASSLDLRGARVDEALEALSRYLDDAALAGLEQVLIIHGLGTGALRDAVREQAAANPLV
                     KGIRPGERGEGGDGATIVRL"
     gene            complement(739..>3464)
                     /locus_tag="AC162A_07745"
     CDS             complement(739..>3464)
                     /locus_tag="AC162A_07745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR5010772.1"
                     /translation="LGDRSRPRGSGATAAKAVAVAIPLFLFFVLAVVGVAGATASVAA
                     YTYLARDLTDPKAVLDDLGFTQQTTVVDRTGRVQLARLGDDRREVVTFADIPPGLIDA
                     TTSYEDKTFWDNSGFDPAGFIAAAIDTIQGRDRGGSTITQQLVRARLLPPTAFEGSVY
                     ERKAKEIIQSIRLTEAYPGLTGKQVIIEKYLNQNFYGNRSYGVAAAARSYWNKDLKDL
                     TLAQMALLAGIPQSPTRLDLVTNAVEETYKDSHGDEQEHLVVPPTTEIVQRRNLILEY
                     MRTRGVLTNNPDGFDYLGTHYPAYTDADYEAAKAEPVILGSQATARWRAPQFVWQVRN
                     ELGEILCGDASECQKIDTGGYQVFTTLDYRMQRIVEKWVFAAAMVPNSNNQDKILRNR
                     GIPRREWSWIKQLTGHNIHNAASGVVDYRTGEVLAYAGSASFTAKGNKKFQPQFDVLG
                     SGWRQPGSSIKPLVYLTGLNDETMTAATMFMDVVTNFAPSGAKAFYPTQADSAERGPV
                     RLRSALQFSLNIPAIKAGIINGIDHTYNKFQDYGLTFQDGTSPVVSESIGTLGTHPID
                     MISAYGAIANGGVLMPRHMIRKVLDENGTQVWPLNDAKPSGKRVASRGAAYIMTDILA
                     GNTQLSVNPFWGKWRITDGVSGSRVRPAAYKTGTTNENRDVHAYGYLAPPTKKDLPAL
                     VAGVWMGNSDNSPNDGKLSLDTSAPLWSAILSDVSKGMPIEGFGRTKPKGLETARVDA
                     FTGMKPGGGGRRTVEELFLPGTAPKRSANAGVVIDVDAASGLRWRDGCIGPKVARTFI
                     DFSNVESGYRSWQRADIAWQARAARGPGVSGGPKGTHTAYLYGSGFYPFGASWGGRFA
                     PSKTCKLAPPPPTPCISIDPFNPCPTAPPEETPSPPTNQGGGNGIGFPPRP"
BASE COUNT          487 a         1137 c         1284 g          556 t
ORIGIN      
        1 cgcaaggccc gcctggagcg cgacgagtcc gtgcgcaccg cccgcgcgga ggccgagaag
       61 ctcgtggaca cgctccgcga cgacgtcgcc gggatccgca agcggctgga gcgtgagacg
      121 gtgacggcgc ccgtgatcga cgatgccgtg gcccacgcgg agaggactct cgagcgcctg
      181 ccggcacgcg agcccgaggc ggagccgacg gagcgaccgg agcccgccga gcctcacgtg
      241 tggcgcctcg gcgaccgcgc gcgcagccgc agcggtggct gggaggggcg gatcgcggcc
      301 ctcgagaagg gtggcacgcg ggcgacgctg gaggcgggcg ggatgcgggt atcggtcaac
      361 gtcgacgacc tcgagccggc ggtgggcgtg ggaggcggca ccgacgggga ccgcgcatca
      421 gcgcgcagcg gcggggcatc gggcgtcggc gcgctgcagc tgtcgaaggc ccggaccgta
      481 gcctcgtccc tggacctgcg cggcgcccgc gtcgacgagg cgctcgaggc gctgtcgcgc
      541 tacctcgacg atgcggcgct ggccggcctc gaacaggtgc tcatcatcca cggcctcggg
      601 acgggcgccc tgcgcgacgc cgtccgcgaa caggccgcgg ccaatccgct ggtgaagggc
      661 atccggcccg gcgagcgggg cgaaggcggg gacggcgcga cgatcgtgcg cctgtaggcc
      721 ggatcggccc cgcggtcctc agggcctggg cgggaacccg atgccgttcc cgccgccctg
      781 gttggtgggc ggtgacgggg tctcttcggg cggcgccgtc gggcacgggt tgaatggatc
      841 gatcgagatg cacggcgtcg gcggcggagg cgcgagcttg cacgtcttgc tcggtgcgaa
      901 ccggccgccc cacgaggcgc cgaacgggta gaagccgctg ccatagaggt aggcggtatg
      961 cgtgcccttg gggccacccg agacgccagg gccgcgcgcc gcgcgcgcct gccaggcgat
     1021 gtccgcacgc tgccacgagc ggtagcccga ctcgacgttg ctgaagtcga tgaacgtgcg
     1081 agcgaccttc ggtccgatgc agccgtcccg ccagcggagg cccgacgccg cgtcgacatc
     1141 gatgaccaca ccggcgttcg cagaccgctt gggcgccgtc cccggcagga acagctcctc
     1201 gacggtcctg cggccgccgc cgcccggctt catgccggtg aacgcgtcga ccctcgcggt
     1261 ctcgagcccc ttcggcttgg tccgcccgaa gccctcgatc ggcatcccct tcgagacgtc
     1321 ggacaggatc gccgaccaca gcggcgccga cgtgtcgagc gacagcttgc cgtcgttggg
     1381 cgagttatcc gagttgccca tccacacgcc cgcgaccagg gccggcagat ccttcttggt
     1441 gggcggggcg agatagccgt aggcgtggac gtcgcggttc tcgttggtcg tgccggtctt
     1501 gtacgcagcc gggcggacgc gggagccgct cacgccgtcg gtgatgcgcc acttgcccca
     1561 gaacgggttg acgctgagct gcgtgttgcc cgcgaggatg tcggtcatga tgtaggccgc
     1621 gccgcgcgag gcgacccgct tgcccgaggg cttggcgtcg ttcagcggcc agacctgcgt
     1681 gccattctcg tcgagcacct tccggatcat gtggcgcggc atcagcaccc cgccgttggc
     1741 gatcgccccg tacgcgctga tcatgtcgat cgggtgggtg ccgagcgtgc cgatgctctc
     1801 cgagaccacc ggggacgtcc cgtcttggaa cgtcaggccg tagtcctgga acttgttgta
     1861 ggtgtggtcg atcccgttga tgatccccgc cttgatcgcc gggatgttga gcgagaactg
     1921 gagtgcgctg cggagccgca cggggcctcg ctcggcgctg tcggcctggg tcggatagaa
     1981 cgccttcgcc ccggacggtg cgaagttggt gacgacgtcc atgaacatgg tcgccgcggt
     2041 catcgtctcg tcgttgaggc cggtgaggta tacgagcggc ttgatcgaag acccgggctg
     2101 ccgccagccg cttcccagga cgtcgaactg gggctggaac ttcttgttgc ccttggcggt
     2161 gaacgacgcc gacccggcgt aggcgaggac ttcgccggtc cggtagtcga cgacgcccga
     2221 ggccgcgttg tggatgttgt gacccgtgag ctgcttgatc cagctccact cgcggcgcgg
     2281 gatcccgcgg ttccgcagga tcttgtcctg gttgttcgag ttggggacca tggctgccgc
     2341 gaagacccac ttctcgacga tccgctgcat ccggtagtcg agcgtggtga acacctggta
     2401 cccgccggtg tcgatcttct ggcactcgga cgcatcgccg cacaggatct cgccgagctc
     2461 attgcgcacc tgccaaacga actgcggtgc acgccagcgc gccgtcgcct gtgagccgag
     2521 gatgacgggt tcggccttcg cggcctcgta gtcggcgtcg gtgtaggcgg ggtagtgcgt
     2581 gcccaggtag tcgaagccgt ccggattgtt ggtgagcacg ccgcgcgtcc tcatgtactc
     2641 gaggatgagg ttccggcgct ggacgatctc ggtcgtcggc ggaaccacga gatgctcctg
     2701 ctcgtcgccg tgggagtcct tgtaagtttc ctcgacggcg ttcgtgacca ggtcgaggcg
     2761 cgtgggcgac tgcgggatgc ccgcaagcag ggccatctgc gcaagcgtca ggtccttgag
     2821 gtccttgttc cagtagctgc gcgcggcggc ggcgacgccg tagctccggt tcccgtagaa
     2881 gttctggttg aggtacttct cgatgatcac ctgcttgccg gtcaggcccg ggtaggcctc
     2941 ggtcaggcgg atcgactgga tgatctcctt cgccttccgt tcgtagacgc tgccctcgaa
     3001 ggcggtgggg ggcagcaggc gcgcgcgcac gagctgctgg gtgatggtcg agccaccccg
     3061 gtcacggccc tggatcgtgt cgatcgccgc ggcgatgaag ccggcggggt cgaagccgga
     3121 gttgtcccag aacgtcttgt cctcgtagga cgtcgtcgcg tcgatcaaac cgggcgggat
     3181 gtccgcgaag gtcacgacct cgcgccgatc gtcgccgagg cgggccagct gcaccctgcc
     3241 cgtccgatcg acaacggtgg tctgctgcgt gaagccgagg tcgtcgagga ccgccttggg
     3301 atcggtcagg tcgcgggcga ggtacgtgta cgcggcgacg gaggccgtcg caccggcgac
     3361 gcccaccacg gcaaggacaa agaacaggaa gagggggatc gctaccgcga cggccttggc
     3421 cgcggtggca ccgctcccgc gagggcggct gcggtcaccg agcc
//
LOCUS       DBNHXO010000375         2191 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_703576, whole genome shotgun sequence.
ACCESSION   DBNHXO010000375 DBNHXO010000000
VERSION     DBNHXO010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_703576"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2191
                     /locus_tag="AC162A_07750"
     CDS             <1..>2191
                     /locus_tag="AC162A_07750"
                     /inference="COORDINATES: protein motif:HMM:NF037626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR5010773.1"
                     /translation="RNIAEKVAREWAERRAASELTDKELDLEKATVDAEVELLDPAAR
                     HAANGESGVATARPKRAAATRSSDRAGSSSRGAGRRVPDLAVLPRLLHDTGAFGSLRE
                     RLGRAGVEPGQHGKHVGLTSVPHGAKSYLAAALALAPDAERICWVARDAEIGDRVAEE
                     LGAWLGDPNLVAVLEPRTSLAYERSELVPDETAARVAALAQWRAGKAKVLVASVQALV
                     QATLAPDDLPAEIRTLRAGQRIGHDQLLAELLERGYSPVIEVAGRGEFARRGGIVDVF
                     PPSAPLPIRVELFGDEIDSMRAFDPTDQRSVEAVREITLLPATEFLLPAGGAEEIRQR
                     LGRIAARLPDRLAQDLARFAGDAASGAKVVEGRALPAGDAAEVWARLVAPNTGLDHLD
                     PSTLLVLDEPGDLADAADFLWRQAEERHAEMVDAGDLPRDWPSSYLPPRDWKSRLHGS
                     RTLELTWQSEAGAADGMAFASKSLTSGDQFGWREPVLPPGLTARLVDGVEHWIGEKAR
                     VVLASDQAPRLAELLGEAGHAVGIVHRVTEAPPPGAIALIERSLNGGFEGGPDGLALV
                     TDRELFGTVRVRRPKAMRRVVPRDLLERLSPGDVVVHIDHGIARYEQMLRRGGEGQER
                     DYLELSFAAGDRIFVPVEQINRVSRYSGGEHPTLSRLGGTDWLRTKQRVRKAVSDLAE
                     ELLALYAKREAAQGFAYAPDSPWQSEMEASFPYEETPDQLRAAEEVKA"
BASE COUNT          298 a          826 c          796 g          271 t
ORIGIN      
        1 agcggaatat cgcggagaag gtcgcccgcg agtgggccga gcggcgcgcc gcctcggagc
       61 tcacggacaa ggagctggac ctcgagaagg ccacggtcga cgccgaggtc gagctgctcg
      121 atccggcggc aaggcatgcc gccaacggcg agtcaggcgt cgcgacggcg agacccaagc
      181 gagctgccgc gaccaggtca tcggatcgtg ccgggtcatc gtcccgtggc gccggccgcc
      241 gggtcccgga tctcgcggtg ctgccgcgcc ttctgcacga caccggcgcg ttcggaagcc
      301 tccgggaacg cctcggccga gcgggcgtcg agccggggca gcacggcaag cacgtcggcc
      361 tgacctccgt cccgcacgga gccaagtcgt acctggccgc agcgctcgcg ctcgcgccgg
      421 acgcggagcg catctgctgg gtggcgaggg acgccgagat cggcgaccgc gtcgcggagg
      481 agctgggcgc atggctcggc gaccccaacc tcgttgccgt gctggagccc aggacctcgc
      541 tcgcgtacga gcggagcgag ctggtccccg acgagaccgc ggcccgcgtc gcggccctcg
      601 cccagtggcg cgccggcaag gcgaaggtcc tcgtcgcgag cgtccaggcg ctggtccagg
      661 cgaccctcgc gcccgacgac ctgcccgcgg agatccggac gctgcgcgcc ggccagcgca
      721 tcggtcacga ccagctcctc gccgagctcc tcgagcgcgg ctactcgccc gtgatcgagg
      781 tcgccggaag gggcgagttc gcgcgccgcg gcggcatcgt cgacgtcttc cccccgagcg
      841 cacccctgcc catccgcgtc gagctgttcg gcgacgagat cgactcgatg cgcgccttcg
      901 accccaccga ccaacgcagt gtcgaggccg tccgcgagat caccctcctg cccgccactg
      961 agttcctgct cccggccggc ggtgccgagg agatccgcca gcggctcggc cggatcgcgg
     1021 cgcgcctccc cgaccggctc gcgcaggacc ttgcgcgctt cgcgggcgac gcggcgagcg
     1081 gggcgaaggt cgtcgagggc cgcgcgctcc ccgcgggcga cgccgccgag gtctgggccc
     1141 gcctcgtcgc ccccaacacg ggcctcgacc acctcgaccc gtcgaccctc ctcgtcctcg
     1201 acgagcccgg cgacctcgcc gacgccgccg acttcctgtg gcgtcaggcc gaggagcggc
     1261 acgcggagat ggtcgacgcc ggcgacctgc ccagggactg gccgtcctcg tatctcccgc
     1321 cgcgagactg gaagagccgc ctgcacgggt cgcgcacgct ggagctgact tggcagtccg
     1381 aggccggcgc ggccgacggg atggcgttcg cctcgaagag cctgaccagc ggcgaccagt
     1441 tcggctggcg cgagccggtg ctcccgccgg gtctgaccgc gcggctggtc gacggcgtcg
     1501 agcactggat cggggagaag gcccgcgtgg tgttggcgag cgaccaggca ccgcggctcg
     1561 cggagctcct cggggaggcg ggccacgccg tcggcatcgt ccatcgcgtg accgaggccc
     1621 cgccgcccgg ggcgatcgcc ctcatcgagc ggagcctcaa cggcggcttc gagggcggtc
     1681 ccgacggcct cgccctcgtc acggaccgcg agctgttcgg cacggtccgg gtgcgcaggc
     1741 ccaaggccat gcggcgagtc gtcccccgcg acctcctcga gcgcctctcg cccggcgacg
     1801 tcgtcgtgca catcgaccat ggcatcgccc gctacgagca gatgctccgg cgtgggggcg
     1861 agggccagga gcgcgactac ctcgagctct cgttcgcggc cggcgaccgg atcttcgtcc
     1921 ccgtcgagca gatcaaccgg gtgtcccggt attccggcgg tgagcacccg acgctcagcc
     1981 gcctcggcgg cacggactgg ctgcggacca agcagcgggt ccgcaaggcg gtcagcgacc
     2041 tcgccgagga gctgctcgcg ctgtacgcca agcgcgaggc tgcgcagggc ttcgcgtatg
     2101 cgccggactc gccgtggcaa tccgagatgg aggcctcgtt cccgtacgag gagacgcccg
     2161 accagctgcg cgccgccgaa gaggtcaagg c
//
LOCUS       DBNHXO010000376         1902 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2291886, whole genome shotgun sequence.
ACCESSION   DBNHXO010000376 DBNHXO010000000
VERSION     DBNHXO010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2291886"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="AC162A_07755"
     CDS             complement(<1..581)
                     /locus_tag="AC162A_07755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5010774.1"
                     /translation="MTAAPAPAGTDVTTAGLVPRLSYFFPAHNEEANLEGLVVEALET
                     LPRIAAEFEIIAVNDGSKDATAEIANRLAAEHPGVVRVVHHAVNRGYGGALRSGFEAS
                     RYELLAFTDGDRQFRIGDLGRLTARLIEPDRPDVVVGYRIKRADPIIRVVYARTYKLA
                     NRIFFGLKVRDVDCACKLFRREALEGVRVESGGA"
     gene            complement(578..>1902)
                     /locus_tag="AC162A_07760"
     CDS             complement(578..>1902)
                     /locus_tag="AC162A_07760"
                     /inference="COORDINATES: protein motif:HMM:NF022481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein O-mannosyl-transferase family"
                     /protein_id="HIR5010775.1"
                     /translation="SAVGMSVLVTRRLGVPVVVAIAAAIGFAVSPIVWRVAVAADAHA
                     LHAALLVAEVALLLRWESFVEASREAPDDPRLGSRADRAIVVAAAVFGVALANHALTL
                     LLIPAVGLFVLAVDRGILRRPRLIAAALGTCLAVVVLFYLELPLRAGPFRAPLVYGHP
                     ETWSGFWDVVAARQFQGDLGGPLADLGGRARAFMTLAFNQFGLWLLLVPSGLVITAMR
                     RPRYALLSGVALLTTCLFAASYSNAAIERYYIGPVFFAWTWLAVLGGAITERVVDAVV
                     TRTSPAGTGSTGRGPADRRRAAGALVGFLVGIVLLLPAAGQIQSRWRVADLSNVTWPH
                     AWLDEAFTTLEPNAVVLSWWSYSTPLWYGQFVEHRRPDVWVVDDRTRLDEHLGEIADV
                     IEANLDTRPVYLIPVAPSDVEKLGARYVIELAEPPGNLYRVVRRQEAP"
BASE COUNT          318 a          668 c          694 g          222 t
ORIGIN      
        1 gcgccgcccg attcgacccg gacgccctcc agcgcttcgc ggcggaacag cttgcaggcg
       61 cagtcgacgt ccctgacctt gaggccgaag aagatgcggt tggcgagctt gtaggtgcgg
      121 gcgtagacga cgcggatgat cgggtcggcg cgcttgatcc gatagccgac gacgacgtcg
      181 ggccggtccg gctcgatcag ccgcgcggtg aggcgcccaa ggtccccgat gcggaactgg
      241 cgatcgccgt cggtgaacgc gaggagctcg tagcgggacg cctcgaagcc cgatcgcagc
      301 gcgccgccgt agccgcggtt gaccgcgtgg tggacgaccc gcacgacgcc cggatgctcc
      361 gccgcgagcc ggttcgcgat ctccgccgtc gcgtccttcg agccgtcgtt gacggcgatg
      421 atctcgaact cggcggcgat gcgcggcaac gtctcgagcg cctccaccac caggccttcg
      481 aggttcgcct cctcgttgtg ggcggggaag aagtaggaca ggcggggcac gagcccggcg
      541 gtcgtgacgt ccgtgcccgc cggcgcggga gcagcggtca tggagcctcc tggcggcgga
      601 ccacccgata aaggttgccg ggcggctctg ctagttcgat cacgtagcgg gcgccgagtt
      661 tctcgacatc cgatggtgcg accggaatga ggtagacggg tcgcgtgtcg aggttagcct
      721 cgatcacgtc ggcgatctcg cccaggtgct cgtcgagccg tgtccggtcg tccaccaccc
      781 acacgtcggg gcggcggtgc tcgacgaatt gtccatacca caggggcgtc gagtacgacc
      841 accacgacag cacgacggcg ttgggctcga gcgtcgtgaa cgcctcgtcc agccacgcat
      901 gcggccaggt gacgttcgac aggtccgcga cccgccaccg ggactggatc tggcccgccg
      961 ccggcagcaa cagcacgatg cccaccagga acccgacgag agcaccggcg gcgcgccgcc
     1021 ggtccgccgg tccccggccc gtcgatccgg ttccggccgg cgacgtgcgg gtcacgaccg
     1081 catcgaccac ccgctccgtg atcgcgccgc cgaggaccgc gagccaggtc caggcgaaga
     1141 acacgggccc gatgtagtag cgctcgatgg cggcgttcga gtacgaggcg gcgaacaggc
     1201 acgtggtcag cagggcaacc cctgagagca gggcgtaccg cggccgccgc atggccgtga
     1261 tgaccaggcc cgatgggacc agaagcagcc acaggccgaa ctggttgaag gcgagcgtca
     1321 tgaacgcacg cgcccggccg ccgaggtcgg cgagcggccc gccgagatcg ccctggaact
     1381 ggcgcgcggc cacgacgtcc cagaagccgc tccaggtctc gggatgtccg tagaccagcg
     1441 gcgcccggaa cgggcccgct cgcaggggca gctcgaggta gaacaggacg acgacggcga
     1501 gacacgtgcc gagcgcggcc gcgatcaggc gcggacggcg gaggatgccg cggtcgaccg
     1561 cgagcacgaa caggccgacg gcggggatca acagcagcgt cagcgcgtgg ttcgcgagcg
     1621 cgacgccgaa cacggccgcg gccaccacga tcgcgcggtc ggcgcgggac cccaggcggg
     1681 ggtcgtccgg cgcctcacgg gacgcctcga cgaacgactc ccagcgcagg agcagggcga
     1741 cctcggcgac cagcagggcc gcatgcaggg cgtgtgcgtc ggccgcgacc gccacccgcc
     1801 acacgatcgg cgagacggcg aagccgatgg ccgcggcgat cgccaccacg accgggaccc
     1861 ccagccgacg ggtcacgagc acgctcatcc cgaccgccga cg
//
LOCUS       DBNHXO010000377         2865 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2091767, whole genome shotgun sequence.
ACCESSION   DBNHXO010000377 DBNHXO010000000
VERSION     DBNHXO010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2091767"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="AC162A_07765"
     CDS             complement(<1..667)
                     /locus_tag="AC162A_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010776.1"
                     /translation="MGRTVMIPVIEVGERTPTASVKPRPPVEMAPLVIAPAAVGLAIS
                     ADDTRPVGRGASSGGAGRGDAAAAAALPGADPCGTTRVLMEERCAVASTAAEQARIAA
                     EALREAQRAYDTLRERVERAQAEADPRGLAAAKDALHMEFRAATARSRGADDTEAAAR
                     EWLTRINELNIRARDALRLAEAGSAELRAALPRLERMGVEADAARISAEMAESTCHEA
                     RVQL"
     gene            1446..>2865
                     /locus_tag="AC162A_07770"
     CDS             1446..>2865
                     /locus_tag="AC162A_07770"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5010777.1"
                     /translation="MAIDPTQEFLVQPVDASWLQCNIECQEACPVGTNCRGYLNLAAE
                     GRFEEGYILSREPNPVAAMCSYVCSAPCERACRRGDIDRPLAIRAMKRFLVEWHEASG
                     IPDVMPAISPRAERVAVVGAGPAGLAVARELAVKGYQITVFDSLPFGGGTMLVGVPAF
                     RLPREAIEMDVRLVERLGVKFVFDTTIGIDITFDELQRDYDGIAITAGAMNPVYLDVP
                     GADLDGVQYGVDYMKKANLGQEMTVGRNVVVIGGGYTAMDCSRTSLRYGAENVTIAYR
                     RTRSELVVDEEELGETEREGVRLEFLVSPVELIGEDGHLTGVKFIRNKLGEPDASGRR
                     SPVPIEGSEFIVPADTVIPAVSQAADLSFLPVEASFEVNRGRLKVDPATYATNVRGVF
                     ACGDFVTGPTTLIESAGHGKKCAYAIDRYLAGRTDVTIEPNVKITSSWRHDMPELYDV
                     LPRQHIPMAPLPDRMPSVDPSVN"
BASE COUNT          395 a         1051 c         1014 g          405 t
ORIGIN      
        1 cgagctgcac ccgcgcctcg tggcaggtcg actccgccat ctcggcgctg atccgcgcgg
       61 cgtccgcctc cacgcccatc cgctccagcc ggggcagggc ggcccggagc tcggccgatc
      121 cggcctcggc gagccgcagg gcgtcgcggg cccggatgtt gagctcgttg atccgggtca
      181 gccactcgcg cgcggcggcc tcggtgtcgt cggcgccgcg ggaccgggcc gtggccgccc
      241 ggaactccat gtgcagcgcg tccttcgcag ccgcgagccc gcgcgggtcg gcctcggcct
      301 gcgcccgctc gacacgctcg cgcagcgtgt cgtacgcacg ctgtgcctcg cgaagggcct
      361 ccgccgcgat ccgggcctgc tctgcggccg tcgacgcgac cgcgcagcgc tcctccatga
      421 ggacacgggt ggtgccgcag ggatcggcgc ccgggagcgc cgcggcggcc gcggcatcgc
      481 cccggcccgc gccaccggac gatgcgcccc ggcccaccgg ccgggtgtcg tcagcgctga
      541 tcgcgaggcc gacggcggca ggtgcgatga cgagcggcgc catctcgaca ggcggtcgcg
      601 gcttcacgga tgccgtcggc gttcgctcgc cgacctcgat gaccgggatc atgaccgtcc
      661 tgcccatgga agcatcatcg accaagggtt cggtcgtggc aacgggtacc agcacggcga
      721 cggcgcgggg aacggccccc agcgacgcgg gcggcgggac ggcgtcgggt ccccgcgtcg
      781 cctggggcct ggggtcctcc ctcggaccct ggtcgggcgc cggtcgcgtc ggcggtgggc
      841 cgttcgggcg atcgacgggc acggcgaccg ccgtcgatcg cagggtcgcc ggaccagata
      901 gcacgcgtgt tcgatccctg accgccatcg aggcagggct tccggccatg gtgggggtgg
      961 ccgtgaaggt cgcggcgggg ccgtcgaacc aggcgtcgtc ctcgggtgac ccgggccgcc
     1021 gaaggacgcc tccgccggcg gtcgcgacgg cgagtgtgga gcccctgcgg gggatcaagg
     1081 agttcggtgc gatcgtgacg cgcacctctc gccggggtgc gggacgggat cggaccgcct
     1141 ccacgatggc cgcaccgatg gcggcgccga ggacgattcc gagggccaga aaggcaatct
     1201 cggacgggct cacggacctc cttgcgggcc tggaacggtg gatgctgcct gggccgacgc
     1261 gtcggggcca gcgtagctgc gccacctccg cgcgtcaaca cgagccacgc tcgctttccg
     1321 ggtcccgcga ccggtgctgc tgtgcacggg ctgtcgcggc ttgatagatt ccgaagcacg
     1381 gcgcagcacg gcgccgtggt tcccgtcgcc ctgccccgat cccgcaggaa aggcacgtcc
     1441 acttcgtggc catcgacccg acccaggaat tcctcgtaca accggtcgac gcctcctggc
     1501 tgcagtgcaa catcgagtgc caggaagcgt gccccgtcgg caccaactgc cggggctacc
     1561 tcaacctcgc cgccgaaggc cggttcgagg agggctacat cctgtcgcgc gaacccaacc
     1621 cggtcgcggc gatgtgctcc tacgtgtgct cggcgccctg cgagcgggcc tgccgccgcg
     1681 gcgacatcga ccggcccctg gccatccgcg ccatgaagcg gtttctcgtg gaatggcacg
     1741 aggcctctgg gatccccgac gtcatgccgg ccatctcccc ccgcgcggag cgggtcgccg
     1801 tcgtgggcgc cggtccggcc ggcctcgcgg tcgcgcgcga gctcgcggtc aagggctacc
     1861 agatcacggt gttcgacagc ctgcccttcg gcggcggcac gatgctggtc ggcgtccccg
     1921 cgttccggct cccgcgcgag gcgatcgaga tggacgtccg cctggtcgag cggctgggcg
     1981 tcaagttcgt gttcgacacc accatcggca tcgacatcac cttcgacgag ctccagcggg
     2041 actacgacgg gatcgcgatc acggccggcg ccatgaaccc ggtctacctc gatgtccccg
     2101 gcgcggacct cgacggcgtc cagtacggcg tcgactacat gaagaaggcg aaccttggcc
     2161 aggagatgac ggtcgggcgc aacgtcgtcg tcatcggcgg cggctacacg gccatggact
     2221 gctcgcgcac gagcctccgc tacggcgccg agaacgtgac catcgcctac cgccgcacgc
     2281 ggtcggagct ggtggtcgac gaggaggagc tgggcgagac agagcgcgag ggcgtgcgcc
     2341 tcgagttcct cgtcagcccg gtcgagctga tcggcgagga tggccacctc accggcgtca
     2401 agttcatccg caacaagctg ggcgaacccg acgcctccgg gcgccgctcg ccggtgccga
     2461 tcgagggctc ggagttcatc gttccggcgg ataccgtgat cccggcggtc tcgcaggcgg
     2521 cggacctctc gttcctgccg gtggaggcaa gcttcgaggt caaccgcggc cgcctcaagg
     2581 tggacccggc gacgtacgcg accaacgtcc gcggcgtgtt cgcgtgcggc gacttcgtga
     2641 ccggccccac caccctgatc gagtcggccg gccacggcaa gaagtgcgcc tacgccatcg
     2701 atcgctatct cgccggccgg accgacgtca ccatcgaacc caacgtcaag atcaccagct
     2761 cctggcgcca cgacatgccc gagctgtacg acgtcctgcc tcgccagcac atcccgatgg
     2821 ccccgctgcc cgaccgcatg ccgtcggtcg atccgtcggt caact
//
LOCUS       DBNHXO010000378         1625 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1102589, whole genome shotgun sequence.
ACCESSION   DBNHXO010000378 DBNHXO010000000
VERSION     DBNHXO010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1102589"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="AC162A_07775"
     CDS             <1..351
                     /locus_tag="AC162A_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010778.1"
                     /translation="GAGSLPEPDHVGPSEAGGDLATVIIQNSSPESMGIVFSGPDVRV
                     ESLDACPTCETFTDTGPAECPDAGPVGTYLVEPGDYEVVVTATSTGDVTPFHGTWTLS
                     AGEEYSSCFYVVTH"
     gene            390..465
                     /locus_tag="AC162A_07780"
     tRNA            390..465
                     /locus_tag="AC162A_07780"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:423..425,aa:Ala,seq:ggc)
     gene            541..>1625
                     /locus_tag="AC162A_07785"
     CDS             541..>1625
                     /locus_tag="AC162A_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR5010779.1"
                     /translation="MTTTGDSTTDAAAGEGRHRIERYDPGAIEPRWQARWDELALYQT
                     DLANQRDRYYLLTMFPYPSGDIHIGHWYIKTPTDAIARYRRMHGKNVFLPIGFDGFGL
                     PAENAAIKNNINPRDWTTSNIANMRRQLRQMGATFDWSAEVVTCDPEYYKWNQWFFLR
                     FLEAGLAYRANSPVDWCPNDGTLAREQVEGADRHCWRCGAKVEKRALDQWYLRVTDYA
                     DELLDFSEIQFPDPLRIQQANWIGRSSGAEIVFETAPSPHHAGGEELRVFTTRPDTLF
                     GATFMVLAPEHPLVATLTAPERRSDVEAYVAATEAKTEIDRLSADREKTGVAIGADAI
                     NPVNGKRVPIFVADYVLGGYGTGAIMAV"
BASE COUNT          280 a          556 c          558 g          231 t
ORIGIN      
        1 ggcgccggca gcctccccga gcccgaccac gtcggaccca gcgaggcggg tggcgacctg
       61 gccacggtga tcatccagaa cagctcgccc gagtcgatgg gcatcgtgtt cagcgggccg
      121 gacgtccggg tcgagagtct cgacgcctgc ccgacctgcg agacgtttac cgacaccggg
      181 cccgccgagt gccccgacgc gggaccggtc ggcacgtacc tggtcgagcc cggcgactac
      241 gaggtggtcg tgacggccac gagcacgggc gacgtgaccc cgttccacgg cacctggacg
      301 ctcagcgccg gcgaggaata ctcgtcctgc ttctacgtcg tcacgcatta gcgagcgcgt
      361 gagggccggg tatactcgcc gtccggcgtg gggctatagc tcagctggga gagcgtctga
      421 atggcattca gaaggtcagg ggttcgaatc cccttagctc caccaacgcc ttagcaacgc
      481 cgaccgattg acccctcgcc gggaccggcg gggggtctcg ttcactcagg gcagggaacc
      541 gtgaccacca caggcgacag cacgacggac gcagccgcgg gcgagggccg gcaccggatc
      601 gaacgctatg acccgggcgc catcgagccc cgctggcagg cgcgctggga cgagctcgcc
      661 ctgtaccaga ccgacctcgc gaaccagcgt gaccgctact acctgctcac gatgttcccg
      721 tacccgtcgg gcgacatcca catcgggcac tggtacatca agacgccgac cgacgcgatc
      781 gcgcgctacc ggcggatgca cggcaagaac gtgttcctgc cgatcggctt cgacgggttc
      841 gggctgcccg ccgagaacgc ggcgatcaag aacaacatca acccgcgcga ctggacgacg
      901 tccaacatcg cgaacatgcg ccgtcaactg cggcagatgg gcgcgacgtt cgactggagc
      961 gcggaggtcg tcacctgcga ccccgagtac tacaagtgga accagtggtt cttcctgcgc
     1021 ttcctcgagg ctggcctggc gtatcgggcg aactcgccgg tcgactggtg ccccaacgac
     1081 ggcacgctcg cgcgcgagca ggtcgagggc gcggaccgtc actgctggcg ttgtggcgcc
     1141 aaagtcgaga agcgcgcgct ggaccagtgg tacctgcggg tcaccgacta cgcggacgag
     1201 ctgctcgact tcagcgagat ccagttcccc gaccccttgc ggatccagca ggccaactgg
     1261 atcggccgct cgtcgggggc ggagatcgtg ttcgagacgg cgccctcgcc acatcacgcc
     1321 ggcggcgagg agctccgggt gttcacgacg cgtcctgaca cgctgttcgg ggcgacgttc
     1381 atggtgctgg cgccggagca tccgctggtc gcgacgctga ccgcgccgga gcgccggagc
     1441 gacgtggagg cgtacgtcgc cgccaccgag gcgaagaccg agatcgaccg gctgtccgcg
     1501 gatcgcgaga agaccggcgt cgcgatcggc gcggacgcga tcaacccggt caacggcaag
     1561 cgcgtcccga tcttcgtcgc ggattacgtg ctgggcggct acggcaccgg cgcgatcatg
     1621 gcggt
//
LOCUS       DBNHXO010000379         3436 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1894658, whole genome shotgun sequence.
ACCESSION   DBNHXO010000379 DBNHXO010000000
VERSION     DBNHXO010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1894658"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="AC162A_07790"
     CDS             complement(<1..196)
                     /locus_tag="AC162A_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HIR5010780.1"
                     /translation="MGDTIAQNRRARHDYTVEDTFEAGIVLRGTEIKSIRAHKVSLAD
                     AYARIDRGEAWIVGLHIAPWE"
     gene            complement(593..1948)
                     /locus_tag="AC162A_07795"
     CDS             complement(593..1948)
                     /locus_tag="AC162A_07795"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR5010781.1"
                     /translation="MTFEPAPTSDETTVGAPPSPVEPPATEATRPSAPSRAGILVGAV
                     ALIAVLAGTTLFFAGFSLGRQVALTPGTPTGDAEAFQPFWDTYAAIIDRYAGGEVDRR
                     SLVEGAIKGMIGALGDPYSQYMTSEEFKASLQGISGQFEGIGATIGTVDGTGAVSACT
                     TLGADCRLAIVAPLTGSPAESAGLLAGDVIDAIDGASLIGLTVDEARGKVRGPKDTTV
                     VLRIQRGLADPFDVSIVRAVIVQPEVETRDLANGDVGYIKLAGFSDRAAEEFDAAVAA
                     AVESGQEALIVDLRGNPGGFVTAARDIASQFLSEGTVFWQEDADGNLTETVVEPGGAA
                     TDPSIRLVVLVDGGSASASEIVAAALHDRGRATLVGTKTFGKGTVQQWTQLEDDNGGF
                     RLTTAKWLTPDKAWIHRVGIEPDVLVTDAPARPGDDPAIDAALRALGEEPAASLARRA
                     A"
     gene            complement(2022..2906)
                     /gene="ftsX"
                     /locus_tag="AC162A_07800"
     CDS             complement(2022..2906)
                     /gene="ftsX"
                     /locus_tag="AC162A_07800"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIR5010782.1"
                     /translation="MIPFVAFSLTRAWQGFWRNALMSLAATLTMVLMLLLLAGFFVLQ
                     NVLLASLSYVEQKVEVVAYIENNATDDQVDTLVATIGAMPEVASVEFVSRDAALARFK
                     EAQAAQGRQDLTASLDSNPLYASVNVKLRSPADLDSITTTLRTDPLVRNVLNIEALVD
                     RVLTVTTFVRTAGVVMVAIVGVIVLFIIVNTIRLAVVARAEEIEIMRLVGASDSFIRW
                     PFVFEGALVGLFGALLTLALLAFAAEPLSQAMVGFFNVLPLELGSVGRDTAMLVLATG
                     VGLGVLGSWLSVRTYLIR"
     gene            complement(2910..>3436)
                     /locus_tag="AC162A_07805"
     CDS             complement(2910..>3436)
                     /locus_tag="AC162A_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR5010783.1"
                     /translation="TKGMVVVDGDDLARLNRRQVPKVRRKIGIVFQDFKLLPRKSVWE
                     NVAFALEVTGTPRRRIRPAVDRVIALVGLTGQAQQLPTQLSGGEQQRTAIARALVHDP
                     RIIIADEPTGNLDPVISWEIIQLLLRINELGVTILMATHDAEIVTALRRRVVALEDGR
                     IIRDDIGATYHRED"
BASE COUNT          528 a         1217 c         1182 g          509 t
ORIGIN      
        1 tctcccacgg ggcgatgtgg agcccgacga tccaggcctc gccgcgatcg atgcgcgcgt
       61 aggcgtccgc gaggctcacc ttgtgagcgc ggatgctctt gatctcggtg ccgcgcagca
      121 cgattcccgc ctcgaacgtg tcctcgacgg tgtagtcgtg ccgggctcgt cggttctggg
      181 cgatggtgtc gcccatcagg tgctcctctg cgctgctcag gggtgggagt gtcgcacggg
      241 ctcgggagat gcccgcggcg gacgcacgaa cgccggcccg tttccggacc ggcgttcgaa
      301 ttgctaccga ccactcgtcg ggatcgtccc tagacgacga tctccgccga ggtggtgcag
      361 caactacaac tgtcgatcac tgcacatcac ctcctttcgt tcataacgaa cgtaacgcaa
      421 acgccgcgcc ggtgcaagcg gctcgtacac gcggcgcgcg cgccacgctg aacgcgcagc
      481 gttcacttga gcggtcgatc cgccgaacgg aacgctcagc gaccgaacca gcgggggaac
      541 cgctggaccg aacgcccagc gttcgttgac cggcgggtaa gaccgctgcc agtcaggcgg
      601 cgcgccgcgc gagcgaggcc gccggttcct cacccagggc cctgagtgcg gcgtcgatcg
      661 cggggtcgtc gccgggccgg gcgggcgcgt cggtcacgag gacgtcgggc tcgatgccga
      721 cccggtggat ccaggccttg tcgggcgtca gccacttggc ggtcgtgagc cggaacccgc
      781 cgttgtcgtc ctcgagctgg gtccactgct ggaccgtgcc cttgccgaag gtcttcgtcc
      841 cgaccagcgt cgcccgtccg cgatcgtgca gggcggccgc gacgatctcg gacgccgacg
      901 ccgagccgcc gtccaccagc accacgagcc ggatcgacgg gtccgtcgcc gcgccgcccg
      961 gctccaccac ggtctcggtc aggttcccgt cggcgtcctc ctgccagaaa accgtgccct
     1021 cggacaggaa ctggctggcg atgtcacggg ccgcggtcac gaagccaccc gggttcccgc
     1081 ggaggtcgac gatcaatgcc tcctgcccgc tttccacggc cgccgcgacc gccgcgtcga
     1141 actcctccgc ggcccggtcc gagaacccgg ccagcttgat gtacccgacg tcgccgttcg
     1201 cgaggtccct ggtctcgacc tcgggctgga cgatgaccgc gcgcacgatc gacacgtcga
     1261 acgggtccgc gaggccgcgc tggatccgga ggaccaccgt cgtgtccttg ggcccccgca
     1321 ccttgccgcg cgcctcgtcg acggtgagcc cgatgagcga ggcgccgtcg atcgcgtcga
     1381 tcacgtcccc cgcgaggagc cccgcgctct ccgccggtga gccggtcaaa ggcgcgacga
     1441 tcgccagccg gcaatccgcg cccagcgtcg tgcacgcgga gaccgcgccc gttccgtcga
     1501 cggtgccgat cgtcgcgccg atgccctcga actggcccga gatgccctgc agggacgcct
     1561 tgaactcctc ggaggtcatg tactgcgagt agggatcgcc gagtgcgccg atcatcccct
     1621 tgatcgcgcc ctcgaccagt gacctgcggt cgacctcgcc gcccgcgtag cgatcgatga
     1681 tcgccgcgta cgtgtcccag aacggctgga acgcctcggc gtcgcccgtc ggcgtgcccg
     1741 gcgtcagcgc cacctggcgg ccgagcgaga accctgcgaa gaacagcgtc gtgccggcga
     1801 gcacggcgat cagcgcgacg gcacccacca ggatgcccgc gcggctcggc gccgaaggtc
     1861 tcgtggcctc ggtcgccggc ggctcgacag gactgggtgg cgcaccgacc gtcgtttcgt
     1921 ccgacgtcgg cgcgggttcg aacgtcatgg ggatgtccgg gtgacgggcg ctggacggga
     1981 gcgcgggccg ccagcgtata cctgcaccat caaacgggcg gtcagcggat gaggtaggtc
     2041 cgcaccgaca gccacgagcc gaggacgccg agaccgaccc ccgtcgcgag cacgagcatc
     2101 gcggtgtcgc ggcccaccga gcccagctcc agcggcagca cgttgaagaa cccgaccatc
     2161 gcctgtgaca gcggctccgc ggcgaacgcg agcagggcga gcgtcagcag cgcgccgaac
     2221 agccccacga gcgcgccctc gaacacgaac ggccagcgga tgaacgagtc cgaagcgccg
     2281 accagccgca tgatctcgat ctcttcggcc cgcgcgacga ccgcgagccg gatcgtgttg
     2341 acgatgatga acagcacgat cacgccgacg atcgccacca tgaccacgcc ggcggttcgc
     2401 acgaaggtcg tgacggtgag cacgcgatcg acgagggcct cgatgttcag gacgttgcgg
     2461 acgagcgggt ccgtgcgcag cgtcgtcgtg atgctgtcga ggtcggctgg gctgcggagc
     2521 ttgacgttga cgctggcgta caaggggttc gaatcgaggg aagcggtcag gtcctgccgg
     2581 ccctgcgcgg cctgcgcctc cttgaaccgg gccagggccg cgtcgcgcga cacgaactcg
     2641 accgaggcga cctcaggcat ggctccgatc gtggcgacca gcgtgtcgac ctggtcgtcc
     2701 gtcgcgttgt tctcgatgta ggcgacgacc tccaccttct gctcgacgta ggacaggctg
     2761 gccagcagga cgttctggag gacgaagaag ccggccagca gcagcagcat caggaccatg
     2821 gtcagcgtcg ccgcgaggct catcagcgcg ttgcgccaga acccctgcca ggcccgggtc
     2881 agcgagaacg cgacgaacgg gatcacgcct cagtcctcgc ggtggtaggt ggcgccgatg
     2941 tcgtcgcgga tgatccgccc gtcctcgagg gccaccaccc tccgccggag cgcagtgacg
     3001 atctccgcat cgtgggtcgc catgaggatc gtcacgccca gctcgttgat ccgcagcagc
     3061 agctggatga tctcccagct gatcacgggg tcgaggtttc cggtcggctc gtcggcgatg
     3121 atgatccggg ggtcgtggac cagggcccgc gcgatcgccg tccgctgctg ctccccgccg
     3181 gagagctgcg tcggcagctg ctgcgcctgc ccggtgaggc cgacgagggc gatgacccgg
     3241 tccactgcgg gccggatccg gcgccgaggc gtgccggtga cctcgagcgc gaacgcgacg
     3301 ttctcccata cggacttgcg gggcagcaac ttgaagtcct ggaacacgat gccgatcttc
     3361 cgtcgcacct tggggacctg gcgccggttg agccgggcga ggtcgtcccc gtccacgacg
     3421 accatgccct tggtcg
//
LOCUS       DBNHXO010000380         3008 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3880711, whole genome shotgun sequence.
ACCESSION   DBNHXO010000380 DBNHXO010000000
VERSION     DBNHXO010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3880711"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="AC162A_07810"
     CDS             <1..1140
                     /locus_tag="AC162A_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012640.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HIR5010784.1"
                     /translation="LAAFIETDLVLVFDHLSHQLSAIASLHTDAPDFEGRYRIAERAI
                     FEALERTARPSQAEMTAAFGRSSETDGAAAPSPFRQVDTSLGRDAYIQAVEHAKDAIA
                     AGEAIQVVLARRQSFDLPNAADGNPIDGIGLYRALRRVNPSPYLFFTRTPSFEVVGAS
                     PELLLQVVGDRMTTHPIAGTRPRGATPADDDRMADELRGDPKERAEHVMLVDLGRNDL
                     GRVARPGTVTTSQYMEVERYSHVLHLVSHVEAKLRPDLDTLDALRAVFPAGTLTGAPK
                     VRAMQLIAAAEGERRGLYGGAVGYLGYDGNLDTAITIRSAVLKGGQAHVHTGAGIVAK
                     SVPESEFEETEHKAAALKRAIELAAATGAPASPAPAPGEPEGAAR"
     gene            1137..1835
                     /locus_tag="AC162A_07815"
     CDS             1137..1835
                     /locus_tag="AC162A_07815"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR5010785.1"
                     /translation="MILMIDNYDSFTFNLVQALEAAGAEVLVVRNDAMTRADVEALAD
                     DPAAGLHGIIISPGPGDPGTAGVSVAVIEVAAERRIPLLGVCLGMQSMAAAYGASIVR
                     APTLVHGEASEIEHDGEGLLAGMPEDFPAARYHSLCVDPATLPADLYVTATSPEDGVV
                     MGLRHRTLPLEGVQFHPESVLTPDGPHLLANFLRLAGEGDGSRLDAVSGSFATRGMAS
                     PEPESPGDPVAAGR"
     gene            1832..2890
                     /gene="trpD"
                     /locus_tag="AC162A_07820"
     CDS             1832..2890
                     /gene="trpD"
                     /locus_tag="AC162A_07820"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR5010786.1"
                     /translation="MSEQVRAALAAIVDGRTLSMDEARLAMGEVMDGEATPSQLAALL
                     MGLRMRGETVDELAGFATAMRERVVRVEAPEGAIDVVGTGGDGSGTFNISTTSALVVA
                     ACGVPVAKHGNRAITSKSGSADVLDALGIRIDHDADSAGASLRDLGFAFLFAPSYHPA
                     MRHAGPTRREIGVRTAFNLVGPLTNPAGTTRQLIGVGAAEAAPRIAEVAARLGTERTF
                     VIHGDGVDELPLDDTGVLYHVGPDGVERHEIKATQLGFTKTATTKLAGGSPEDNARMS
                     ESVLRGEPGARRDVVLLNAGAALLVAGVVEKLEEGIDRAGLTIDAGLASELLGRLREE
                     RRVADAAKAAAATEGAPA"
BASE COUNT          417 a         1081 c         1136 g          374 t
ORIGIN      
        1 ctggcggcgt tcatcgagac cgacctcgtc ctggtcttcg accacctcag ccaccagctc
       61 tcggcgatcg cgtcgctcca caccgacgcg ccggacttcg aaggccgcta ccggatcgcc
      121 gagcgggcca tcttcgaggc gctggagcgg accgcccgcc ccagccaggc ggagatgacc
      181 gccgccttcg ggcggtcgag cgagaccgac ggcgctgcgg cgccaagccc cttccgccag
      241 gtggacacct cgctcgggcg ggacgcctac atccaggccg tggaacacgc gaaggacgcg
      301 atcgccgccg gggaggcgat ccaggtcgtc ctcgcgcgcc gccagtcgtt cgacctcccc
      361 aacgccgcgg acggcaatcc gatcgacggc atcggcctgt atcgggccct gcgccgcgtc
      421 aacccgtcgc cctacctgtt cttcacccgg acgccctcgt tcgaggtcgt cggcgccagc
      481 ccggagctcc tgctccaggt cgtcggcgac cggatgacga cccaccccat cgccggcacg
      541 cggccgcgcg gcgcgacgcc cgccgacgac gaccgcatgg ccgacgagct gcgcggcgac
      601 cccaaggagc gcgccgagca cgtgatgctc gtggacctcg ggcgcaacga cctcgggcgg
      661 gtcgcgcggc ccggcaccgt gaccaccagc cagtacatgg aggtcgaacg ctacagccac
      721 gtcctgcacc tggtgagcca cgtggaggcc aagctgcggc cggacctcga tacgctcgac
      781 gcgctgcgcg ccgtgttccc ggcggggacc ctcaccgggg cgccgaaggt gcgcgcgatg
      841 cagctgatcg ccgcggccga gggcgagcgg cgcggcctgt atggtggggc ggtgggctat
      901 ctaggctacg acggcaacct ggacaccgcg atcacgatcc ggagcgccgt cctgaagggc
      961 ggccaggcgc acgtccatac gggggccggg atcgtcgcca agtccgtgcc cgagtccgag
     1021 ttcgaggaga ccgagcacaa ggccgcggcg ctcaagcggg cgatcgagct ggcggcggcg
     1081 acgggcgccc cggcgtcacc cgcacccgca cccggcgagc cggagggggc cgctcgatga
     1141 tcctgatgat cgacaactac gactcgttca cgttcaacct cgtccaggcg ctcgaggcgg
     1201 ccggggccga ggtgctggtc gtgcgcaacg acgccatgac gcgggccgac gtcgaggcgc
     1261 tggccgacga tccggcggcg ggcctgcacg ggatcatcat ctcgcccgga cccggcgatc
     1321 cgggcacggc cggcgtgtcg gtcgcggtga tcgaggtcgc cgccgagcgg cggatcccgc
     1381 tcctcggcgt gtgcctcggc atgcagtcaa tggccgcggc ctacggcgcc tcgatcgtcc
     1441 gggcaccgac cctggtccac ggcgaggcgt cggagatcga gcacgacggc gagggactgc
     1501 tcgccgggat gcccgaggac ttcccggcgg cgcgctatca ctcgctgtgc gtcgacccgg
     1561 cgacgctccc ggcggacctc tacgtcaccg cgaccagccc ggaggacggg gtggtcatgg
     1621 ggctgcgcca ccggacgctc ccgctcgagg gcgtccagtt ccacccggag tccgtcctca
     1681 cgcccgacgg gccgcacctg ctggccaact tcctgcgcct ggccggtgag ggcgacgggt
     1741 cgcggctgga cgccgtgtcg ggatctttcg cgacgcgcgg catggcctcg ccggagcccg
     1801 agtcgccggg cgatccggtg gcggccggac gatgagcgag caggtccgtg ccgcgctggc
     1861 ggccatcgtc gacggccgga cgctgtcgat ggacgaggcg cgcctggcga tgggcgaggt
     1921 gatggacggc gaggcgacgc cgtcgcagct agcggcgctg ctgatgggcc tccggatgcg
     1981 cggcgagacg gtcgacgagc tggcgggctt tgcgacggcg atgcgggagc gcgtcgtgcg
     2041 ggtcgaggcg cctgaggggg cgatcgatgt ggtaggcacc ggcggcgacg gctcgggcac
     2101 cttcaacatc tcgacgacgt cggcgctcgt ggtcgcggcg tgcggcgtcc cggtggccaa
     2161 gcacggcaac cgcgcgatca cctccaagtc gggttcggcg gacgtgctcg acgccctcgg
     2221 catccggatc gaccacgacg cggactcggc cggcgcctcg ctgcgcgacc tcggcttcgc
     2281 gttcctgttc gccccgtcgt accacccggc catgcgccac gccggcccga cgcgacgcga
     2341 gatcggcgtg cggaccgcgt tcaacctcgt gggtccgctg acgaatccgg ccgggacgac
     2401 gcgtcaactg atcggcgtgg gagcggccga ggcggcgccg cgaatcgccg aggtcgcggc
     2461 tcgcctcggg accgagcgca cgttcgtgat ccacggcgac ggcgtcgatg agctgccgct
     2521 cgacgacacg ggcgtgctct accacgtggg ccccgatggc gtcgagcggc acgagatcaa
     2581 ggcgacccag ctcgggttca cgaagaccgc cacgaccaag ctcgccggag gctcgcccga
     2641 ggacaacgcc cggatgtcgg agtccgtgct gcggggcgaa cccggggcgc ggcgagacgt
     2701 cgtgctgctc aacgccggcg cggcgctgct ggtcgccggc gtggtcgaga agctggagga
     2761 ggggatcgac cgggccgggc tcacgatcga cgcgggcctg gcgtcggagc tgctgggcag
     2821 gctgcgcgag gagcggcggg tggccgatgc ggccaaggcc gcggcagcga cggagggggc
     2881 gcccgcatga cggtcgccgc ggggccgcgc tcgaggtcgg ggagcgtcgt cgacgagatc
     2941 gccgcgcgcc ggaaggccga catcggggcc gagctggacg ccctcggccg cgaagggctc
     3001 cgccgggc
//
LOCUS       DBNHXO010000381         6214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1696163, whole genome shotgun sequence.
ACCESSION   DBNHXO010000381 DBNHXO010000000
VERSION     DBNHXO010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1696163"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="AC162A_07825"
     CDS             complement(<1..736)
                     /locus_tag="AC162A_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013565.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR5010787.1"
                     /translation="MTIRLQDTLTGEVRPLEPIEAGRVGIYSCGPTVYGPAHVGNFRS
                     FLFADLLVRYLRYQGLGVTWVMNLTDIDDKIIRGAAAEGISTTELADRYAARFLADAG
                     TVGMTRPDVLPRATEHIPQIVALVQALLEGGHAYRTEDGSIFFRISSWPAYGRLARLD
                     PEAMRVGERVEADEYGKDDVRDFALWKGPKPGEPSWDTAIGSGRPGWHIECSAMSMAH
                     LGPSFDIHTGGVDLVFPHHEDEIAQSE"
     gene            complement(733..1896)
                     /gene="ispF"
                     /locus_tag="AC162A_07830"
     CDS             complement(733..1896)
                     /gene="ispF"
                     /locus_tag="AC162A_07830"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013308.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR5010788.1"
                     /translation="MGADAVIVAAGASRRMDGVDKLSAVVGGRPLLAWSIAALSASAA
                     VDRVIVVVAPERVDELAAAAWLPAGTEVVRGGATRQASVAAGVRHLALTDPAGGDRPV
                     LVHDGARPLVSVALVDAVAESVERHGAAIPVLSVAETLKRIDGDRIVETVDRRTLAVA
                     QTPQGARRRLLLDAWERYPPDGEREFTDEAALLEACTITVHAIPGQPTNLKVTLPDDL
                     LRVERQLDADGARVGFGHDSHPFGPGSPLRLGGIAIPGAPALAGHSDGDVVLHAVADA
                     LLGAAGLGDLGRLFPSDERTPRGVESTALLGEVVGRLAAAGLRPASVDITVIGARPRL
                     GGSLDAMRAAIASLLGVEVGRVNVKASSGNLSGDDGAGRSMSARAVATLRRAA"
     gene            complement(1923..2978)
                     /locus_tag="AC162A_07835"
     CDS             complement(1923..2978)
                     /locus_tag="AC162A_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HIR5010789.1"
                     /translation="MIRTIRVLGAALGAFIGLGLAAAGTGLFTDLAYAGFILAAWTVA
                     WMIVGFAVLPYFTIVPARWLIEKVQELSTAEFVAAILGLFVGLLLGLLLGLPLAQIEG
                     PLGTWLPLGISLFLGLGMMGLTVAKRFDLIAAAEGAGIIRRPGSGDEANEGDPHVVVD
                     TSVLIDGRIAEIVDSGFIYGTLVVPRFVLEELQHIADSSDALRRNRGRRGLEILARMQ
                     RDPRTPVEVVEDEAPGVAEVDAKLVELAKRRSRVVLTNDFNLNRVAELQGVRVMNINS
                     LANAVKPAVLPGEELRVKVIQEGKEAGQGVGFLDDGTMIVVEGGARLIDRDVDVSVTR
                     VLQTVAGRMIFAQPRLD"
     gene            complement(3078..4481)
                     /gene="radA"
                     /locus_tag="AC162A_07840"
     CDS             complement(3078..4481)
                     /gene="radA"
                     /locus_tag="AC162A_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR5010790.1"
                     /translation="MARIQSRFVCQSCGAAVPRWEGQCRSCGEWNTLVETVVRDQPRN
                     RPVAVTGAAAPVALSQAAEAGEEVRRPVGIGELDRVLGGGLVAGSVVLLGGEPGIGKS
                     TLLLQAAAGIARGASVLYATGEESVAQVRLRAARLGLTSGPAADRIRVVAETSVGRIV
                     DLARAEPTGMLVVDSVQTVASDELDGPPGSVGQVREAALRLMELAKADGVPVVLVGHV
                     TKDGTLAGPKTLEHLVDVVLSVEGDRTGGLRLVRATKNRYGSTEEVGVFEMAERGLVE
                     VADPARAFLADHDGPAPGSVVAPVLEGSRPLLVEVQALVAPSGAPSPRRTASGIDSNR
                     LALLVAVLGRRAGIGLSGHDVYANLAGGLTVGEPGLDLPLALALASSLRDRPLHPGTV
                     AIGEVGLLGELRPVGGLDRRLREAARLGFTRAIVPRSRAHPDPASTGVEITVVGSLAE
                     AIRASLADPIAAVRELV"
     gene            complement(4502..>6214)
                     /locus_tag="AC162A_07845"
     CDS             complement(4502..>6214)
                     /locus_tag="AC162A_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR5010791.1"
                     /translation="YRGEFEERLKKIIEELRNTNDAILFIDELHTLVGAGAAEGAIDA
                     ANILKPPLSRGELQCIGATTLDEYRKYIEKDAALERRFQPVMVEEPTLEQTIEILKGI
                     RERYEQHHKVQITDEAVQAAADLSIRYITDRHLPDKAIDLIDEAASRVRLRHSSAPPE
                     LRAAQKDLERITKEKDAAINAQEYEQAATLREAEATSRESVDKLRADWQSTVSSDTPK
                     VDDEEIAQVVAMWTGIPVTRIAQEESERLLKMEEALHQRLIGQEEAIEIVSRAVRRAR
                     AGLKDPKRPIGSFIFLGPTGVGKTELAKALAEFMFGSEDTLIKIDMSEFMERHNVSRL
                     VGAPPGYVGFDEGGQLTEAVRRKSYAVILLDEIEKAHAEVFNILLQILEDGHLTDAKG
                     RRVDFRNTIIIMTSNLGAAKIQTNSSLGFRQQGDTVETRAAASYDLMKEKVQTELKTN
                     FRPEFLNRIDATVVFRSLTVEEITQIVDLMLKRVRDQLKAQGMILEITQPAKEHLIKL
                     GYDQAYGARPLRRTIQNLIEDPLAEHLLLGRYSQGETIQVDLDPDGNGLVIAAIEAKT
                     PVEA"
BASE COUNT          856 a         2248 c         2224 g          886 t
ORIGIN      
        1 cctccgactg ggcgatctcg tcctcgtgat gcgggaacac gaggtcgacg cccccggtgt
       61 ggatgtcgaa cgacggcccg aggtgggcca tgctcatcgc ggagcactcg atgtgccagc
      121 cgggccgccc ggagccgatc gccgtgtccc acgacggctc gcccggcttc gggcccttcc
      181 acagcgcgaa gtcccgcacg tcgtccttgc cgtactcgtc ggcctccacc cgctcgccga
      241 cgcgcatggc ctcgggatcg aggcgggcga ggcggccata ggcgggccac gacgagatcc
      301 ggaagaagat cgagccgtcc tcggtgcggt aggcatggcc cccctcgagc agcgcctgga
      361 cgagggcgac gatctgcggg atgtgctcgg tcgcacgggg cagcacgtcc gggcgggtca
      421 tcccgacggt tccagcgtcg gcgaggaagc gtgcggcgta gcggtcggcc agctccgtcg
      481 tggagatgcc ctcggccgcg gcgccgcgga tgatcttgtc gtcgatgtcg gtcaggttca
      541 tgacccacgt cacgccgaga ccctggtagc gcaggtaccg caccagcagg tccgcgaaca
      601 ggaacgagcg gaagttgccg acgtgggcgg ggccgtacac cgtcggtccg cagctgtaga
      661 tgccaacccg gccggcctcg atcggctcga ggggtcgaac ctctccggtc agcgtgtcct
      721 ggaggcggat cgtcatgccg cacgccgcag ggtcgcgacc gcgcgggccg acatcgagcg
      781 ccccgcgccg tcgtcgcccg agaggttgcc gctcgacgcc ttgacgttga cgcgtccgac
      841 ctcgacgccg agcagcgatg caatcgccgc gcgcatcgcg tcgaggctcc ctccaaggcg
      901 cggccgcgcg ccgatgaccg tgatatccac cgacgccggc cggagcccgg ccgcggcgag
      961 ccgcccgacg acctcgccca ggagagcggt gctctcgacg ccccgcggcg tccgctcgtc
     1021 gctgggaaac aggcggccca gatcacccag gccggccgcg cccaggagcg cgtccgcgac
     1081 ggcatggagg accacgtcgc catcggagtg acccgcgagc gcgggtgctc ccgggatcgc
     1141 gatgccgccg agccggagcg gtgatccggg gccgaacggg tggctgtcgt ggccgaaccc
     1201 gaccctggcc ccgtcggcgt cgagctgccg ctcaacccgc agcaggtcgt cgggcagggt
     1261 caccttgagg ttggtcggtt gaccgggaat cgcatggacg gtgatggtac aggcctcgag
     1321 cagggcggcc tcgtcggtga actcgcgctc cccatccggc gggtagcgct cccaggcatc
     1381 gaggagaagg cgccggcgcg ccccctgggg cgtctgggcg acggcgagcg tgcggcggtc
     1441 caccgtctcg acgatccggt cgccgtcgat gcgcttcagg gtctcggcca cggacaggac
     1501 cgggatcgcc gccccgtgcc gctccaccga ctcggccacc gcatcgacca gcgccacgga
     1561 gaccagcggc cgggccccgt cgtggacgag gacgggccgg tcgccgccgg cggggtcggt
     1621 cagcgccagg tggcgcacgc ccgcggcgac cgacgcctgg cgcgtggcac cgccccggac
     1681 cacctcggtg ccggcaggca gccaggcggc tgccgcgagc tcgtcgaccc gctcgggcgc
     1741 gacgacgacg atcacacggt cgacggcggc cgatgcggac agcgcggcga tcgaccacgc
     1801 aagcagcggc cgcccaccga ccacggccga gagcttgtcc acgccgtcca tccggcgcga
     1861 cgcaccggcg gcgacgatga ccgcgtccgc acccacctgc ccgtcgcccc ggcgaccgct
     1921 ggtcagtcga ggcgcggctg ggcgaagatc atccggccgg ccacggtctg gaggacgcgc
     1981 gtcacgctca cgtccacgtc gcgatcgatc agccgcgccc cgccctcgac cacgatcatg
     2041 gtgccgtcgt cgaggaagcc gacgccctgg ccggcctcct tgccctcctg gatgaccttg
     2101 acgcggagct cctcgcccgg cagcaccgcc ggcttcacgg cgttcgcgag ggagttgatg
     2161 ttcatcaccc gaacgccctg caactccgcg acccggttga ggttgaagtc gttggtgagc
     2221 acgacccggc tgcgccgctt ggcgagctcg acgagcttcg cgtccacctc ggcgacgccc
     2281 ggcgcctcgt cctcgacgac ctcgaccggc gtgcgcggat cgcgctgcat gcgggccagg
     2341 atctcgaggc cccggcggcc ccggttgcgg cgcagggcgt ccgatgagtc cgcgatgtgc
     2401 tgcagctcct cgagcacgaa gcgcggcacg acgagcgtcc cgtagatgaa cccggagtcc
     2461 acgatctcgg cgatccggcc gtcgatcagc acgctggtgt cgacgacgac gtgcgggtcg
     2521 ccctcgttcg cctcgtcgcc cgagcccggg cggcggatga ttccggcccc ctcggcggcg
     2581 gcgatcaggt cgaaccgctt cgcgacggtg aggcccatca tgccgaggcc aaggaacagc
     2641 gagatcccga gcggcagcca ggtgccgagc gggccttcga tctgggcgag cgggaggccg
     2701 agcagcaggc ccagcagcag gccgacgaac aggcccagga tggcggccac gaactcggcg
     2761 gtggacagct cctggacctt ctcgatcagc cagcgcgccg ggacgatggt gaagtacggg
     2821 agcacggcga agccgacgat catccacgcg accgtccagg cggcgaggat gaacccggca
     2881 taggcgaggt ccgtgaagag tccggtccca gccgcggcaa gcccgagccc gatgaacgcg
     2941 cccagtgccg caccaaggac ccggatggtc cgaatcacgc cactcctcga tcgctccgct
     3001 cgcgccggcg acggacggcc gcgagcgcgc ccgagcctag catgcggcgc cgttcgcccg
     3061 cagcgtgtgg atcaccctca aaccagctcc cggacggcgg cgatcggatc ggccagggag
     3121 gcgcgaatcg cctcggcgag ggagccgacg acggtgatct cgacgccggt cgaggcgggg
     3181 tctgggtggg cgcgtgaccg gggaacgatg gcccgcgtga agcccaggcg ggccgcctcg
     3241 cgcagccgtc ggtcgaggcc tccgacgggg cggagctcac cgagcaggcc cacctcgccg
     3301 atggcgaccg tccccgggtg gagcggccgg tcgcggagcg acgacgcgag cgcgagggcc
     3361 agcggcaggt ccagcccggg ctcgccgacg gtcaggcccc ccgcgaggtt ggcatagacg
     3421 tcgtggcccg acaggccgat ccccgcccgc cgtccgagga cggcgacgag cagcgccagc
     3481 cggttggaat cgatccccga ggcggtccgg cgcggtgacg gtgcgccgct cggggcgacg
     3541 agcgcctgca cctcgaccag gaggggccgc gacccctcga ggaccggcgc gaccacgctg
     3601 cccggggccg ggccgtcatg atcggcgagg aaggcacggg ccgggtcggc gacctcgacg
     3661 aggccccgtt cggccatctc gaacacgccg acctcctcgg tcgacccgta ccggttcttc
     3721 gtcgcgcgga ccaggcgcag cccgccggtc cggtcaccct cgaccgagag cacgacgtcg
     3781 accaggtgct cgagggtctt cgggccggcc agcgtgccgt ccttggtcac gtgcccgacg
     3841 agcacgacgg gcacgccgtc cgccttcgcg agctccatca gccgcagcgc ggcctcccgc
     3901 acctgcccga cgctgccggg cggcccgtcg agctcgtccg atgccacggt ctgcacggag
     3961 tcgaccacga gcatccccgt cggctcggcc cgcgcgaggt ccacgatccg cccgacgctc
     4021 gtctccgcga cgacgcggat gcggtcggcc gccgggccgc tggtgagccc gagccgtgcc
     4081 gcgcgcagcc gcacctgggc cacggactcc tcgcccgtcg cgtagagcac ggaggcgccg
     4141 cgggcgatcc cggctgccgc ctggagcagc aaggtggact tgccgatccc gggctcgccg
     4201 cccaggagga cgaccgaacc ggcgaccagg ccgccaccca ggacccgatc gagctcgccg
     4261 atcccgacgg gccgccggac ctcctcgccc gcctcggcgg cctgggacag cgctacgggg
     4321 gcggccgcgc cggtgaccgc gaccggacga ttccgcggct gatcgcggac gaccgtctcg
     4381 acgagggtgt tccattcgcc gcacgagcgg cactgccctt cccagcgcgg gacggcggcg
     4441 ccgcaggact ggcagacgaa gcggctctgg atgcgggcca ctcgcggctc ccggcccgcg
     4501 gtcaggcttc gaccggggtc ttcgcctcga tggccgcgat gaccaggccg ttgccgtccg
     4561 gatcgagatc gacctggatc gtctcgccct ggctgtaccg gcccagcagc aggtgttcgg
     4621 ccagcgggtc ctcgatcagg ttctggatcg tccggcgcag cggccgggcg ccgtacgcct
     4681 ggtcgtagcc gagcttgatc aggtgctcct tggccggctg ggtgatctcg aggatcatgc
     4741 cctgggcctt gagctggtcg cgcacgcgct tgagcatcag gtcgacgatc tgcgtgatct
     4801 cctcgaccgt gaggctgcgg aacacgaccg tcgcgtcgat ccggttgagg aactcgggcc
     4861 ggaagttggt cttgagctcg gtctggacct tctccttcat caggtcgtac gacgcggccg
     4921 cccgtgtctc cacggtgtcg ccctgctgcc ggaagccgag gctggagttg gtctggatct
     4981 tggccgcccc gaggttcgag gtcatgatga tgatcgtgtt gcggaagtcg acccggcggc
     5041 ccttggcgtc ggtgaggtgt ccgtcctcca ggatctggag caggatgttg aacacctcgg
     5101 cgtgcgcctt ctcgatctcg tcgagcagga tcaccgcgta gctcttgcgt cggacggcct
     5161 cggtcagctg gccgccctcg tcgaacccga cgtagcctgg cggcgccccg acgaggcggc
     5221 tgacgttgtg gcgctccatg aactcgctca tgtcgatctt gatgagcgtg tcctcggagc
     5281 cgaacatgaa ctcggcgagg gccttggcga gctcggtctt cccgacgccc gtggggccga
     5341 ggaagatgaa cgagccgatg ggtcgcttgg ggtccttgag gccggcgcgc gcgcgacgca
     5401 cggcgcgcga gacgatctcg atcgcctctt cctggccgat caggcgctgg tggagggcct
     5461 cctccatctt gagcagccgc tcggactcct cctgggcgat gcgcgtgacc gggatccccg
     5521 tccacatcgc gacgacctgg gcgatctcct cgtcgtcgac cttgggcgtg tcgctcgaca
     5581 ccgtcgattg ccagtccgcg cggagcttgt cgacgctctc acgggacgtc gcctcggcct
     5641 cgcgcagcgt ggccgcctgt tcgtactcct gcgcgttgat cgccgcgtcc ttctccttcg
     5701 tgatccgctc caggtccttc tgggcggccc gcagctcggg cggcgcggat gagtggcgca
     5761 ggcgcacccg gctggccgcc tcgtcgatga ggtcgatggc cttgtcgggc aggtggcggt
     5821 ccgtgatgta gcggatcgac aggtccgccg cggcctgcac cgcctcgtcc gtgatctgga
     5881 ccttgtggtg ctgctcgtag cgctcgcgga tgcccttcag gatctcgatc gtctgctcga
     5941 gcgtgggctc ctcgaccatc acgggctgga agcgccgctc cagcgccgcg tccttctcga
     6001 tgtacttgcg gtactcgtcg agcgtggtgg cgccgatgca ctggagctcg ccgcgcgaca
     6061 gcggcggctt gaggatgttg gcggcgtcga tcgccccctc ggcggcgccc gccccgacca
     6121 gcgtgtgcag ctcgtcgatg aacaggatcg cgtcgttcgt gttgcggagc tcctcgatga
     6181 tcttcttgag gcgctcctcg aactcgccgc ggta
//
LOCUS       DBNHXO010000382         1701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_310370, whole genome shotgun sequence.
ACCESSION   DBNHXO010000382 DBNHXO010000000
VERSION     DBNHXO010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_310370"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="AC162A_07850"
     CDS             complement(<1..503)
                     /locus_tag="AC162A_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5010792.1"
                     /translation="MDKRRYVQRARAASAEVTRLRILDAAEASFDRGPLAAVRIDDVA
                     RRAGVSRSTVYTLFDSRRGLLVALAGRLREVAGFERLIEASMHPDARESLRRAQRESV
                     RIFATRPELARALFTMAGVDADAVAAVAALEDGRRPGMVHLARRLEEQGQLRAGIGVK
                     EAADLLSV"
     gene            579..>1701
                     /locus_tag="AC162A_07855"
     CDS             579..>1701
                     /locus_tag="AC162A_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5010793.1"
                     /translation="MTRRTSTPVLIVGAGPAGLVTALVLARQGVRSTLVERHPGTSTH
                     PRASGVSTRSMEIFRRLGVEDEIRARSLDAIPLMSMSASLASPARQVAPLGFPTSEQA
                     ATVSPTGPTISPQDHVEPVLLRRIEALGLTDVRFSTEVLALRQGASAVETTVVDRTTG
                     ERSVVHAEYVVAADGARSTVRDLLGIDVIGPAGFEHYASILFRANLWPVVGEPRYGLY
                     MLGGPGVRPAIVAPMGPDDRFVFGQPGSPEAVAAMVADPELAKAAIRTAAGVPDLEVD
                     VLASMPLEFGAQLATRWRDGSVFLVGDAAHRMPPFGGRGMNTAVADAYNLGWKLATVL
                     TGVASPALLDSYEAERMPVGRHNVSLALARHPELAARHGL"
BASE COUNT          230 a          652 c          585 g          234 t
ORIGIN      
        1 accgacagga gatcggccgc ctccttgacg ccgatccccg cccgcagctg gccctgctcc
       61 tcgaggcggc gagcgaggtg gaccatgccc gggcgccggc cgtcctcgag tgccgcgacc
      121 gccgcgacgg catcggcgtc gacgcctgcc atcgtgaaca gcgctcgggc gagctcaggc
      181 cgcgtggcga agatccgcac cgactcccgc tgggcccgcc ggagcgactc ccgggcgtcg
      241 ggatgcatcg acgcctcgat caggcgctcg aacccggcca cttcgcgcag ccgtccggcc
      301 agcgcgacca gcagtcctcg ccgcgagtcg aacagcgtgt agacggtcga gcgcgagaca
      361 cctgcccgcc tggcgacgtc gtcgatccgg accgcggcca agggtccgcg atcgaacgag
      421 gcctcggcgg catcgaggat tcgcagccga gtgacctccg cggaggccgc ccgggcgcgt
      481 tgcacatagc gacgtttgtc catgttgaca gcatgatact tcatcggaca gactgtgcat
      541 cggaatacac actgtccgat gaaatggaga ttgacgccat gacccgccgc accagcaccc
      601 cggtcctcat cgtcggcgcc ggcccggccg gcctcgtgac cgccctcgtc ctcgcccgcc
      661 agggcgtccg ctccaccctc gtggagcgcc atccggggac gtcgacccac ccgcgcgcct
      721 cgggcgtgag cacgcgatcc atggagatct tccggcgcct gggtgtcgag gacgagatcc
      781 gcgcccgcag cctcgacgcc atcccgctga tgtcgatgtc ggcttcgctc gcatcgccgg
      841 cgcgccaggt cgccccgctc ggcttcccca cctcggagca ggccgcgacg gtgagcccga
      901 ccggtccgac gatcagcccc caggaccacg tggagccggt gctgctccgc cggatcgagg
      961 ccctcggcct gaccgacgtc cggttctcga ccgaggtcct cgcgctccgc cagggcgcct
     1021 ccgcggtcga gacgacggtc gtcgatcgca cgacgggcga gcgctcggtc gtgcacgcgg
     1081 agtacgtggt cgccgcggac ggcgcccgca gcacggtccg agacctgctc ggcatcgacg
     1141 tgatcggccc ggccgggttc gagcactacg cgtcgatcct gttccgcgca aacctgtggc
     1201 ccgtcgtcgg cgagccgcgc tacgggctgt acatgctcgg cgggccgggc gtccgcccgg
     1261 caatcgtggc cccgatgggc cccgacgacc ggttcgtgtt cgggcagccg ggcagccccg
     1321 aagcagtcgc ggcgatggtc gccgatcccg agctcgcgaa ggccgcgatc cgcacggcgg
     1381 ccggtgtgcc ggacctcgag gtcgacgtcc tcgcgtcgat gccgctcgag ttcggtgccc
     1441 agctggccac ccgctggcgg gatggctccg tgttcctcgt gggcgacgcc gcccaccgga
     1501 tgccgccgtt cggtggccgg ggcatgaaca ccgcggtggc cgacgcgtac aacctcggct
     1561 ggaagctcgc gaccgtcctc acgggcgtcg ccagccccgc cctcctcgac tcgtacgagg
     1621 ccgagcggat gcccgtcggc cggcacaacg tgtcgctggc gctggcccgc cacccggagc
     1681 tcgcggcacg gcacgggctg g
//
LOCUS       DBNHXO010000383         1534 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1301168, whole genome shotgun sequence.
ACCESSION   DBNHXO010000383 DBNHXO010000000
VERSION     DBNHXO010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1301168"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="AC162A_07860"
     CDS             <1..939
                     /locus_tag="AC162A_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HIR5010794.1"
                     /translation="IGGIVTGSRTVITKSKSTMAVVTLEDLQGTLEVVVFPRTYEQTI
                     GTWREGAILLVAGRVDHRGEEASLLADAVWDWDTVADRGAEAFAREVGALDRRGGGRR
                     GGPGAPGSTNGHGNGNGNGYGAPRDPVAVGPGRGAVVPAGAEATAVRVSPTRAADPFD
                     VAVGPSSVTEEPDLPRVAPGEPIATYAEPPDLANAAMAGGAEEAEEPPLPDEQRNVAA
                     AAAQAPSDPVDASPGAVLNVRFARDAGSERVVSAMQAFKSVLRERPGGTRVVVHVPAP
                     GGSALPMELRGVAYDAELLAELRRRVGEGVIDLSLG"
     gene            complement(1005..>1534)
                     /locus_tag="AC162A_07865"
     CDS             complement(1005..>1534)
                     /locus_tag="AC162A_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010795.1"
                     /translation="RSSDRVMGEPHTMSASTPTASSTFWTTTQPQRGVASRLTAGFLF
                     ITTGVVLASAVLGLSVASAIIARGIVVADAGDVATIRALTPLFALFGVIAAAHLVAGF
                     GMAFGSRQAASLGIGLGAFNVVAGVVGLIVAATGSRNQADGVGIAMTFVVMGIVLTVA
                     ARAADWNTHGPVDEA"
BASE COUNT          208 a          530 c          616 g          180 t
ORIGIN      
        1 atcggcggga tcgtgaccgg atcccggacg gtgatcacga agtcgaagtc gacgatggcg
       61 gtcgtgacgc tcgaggacct gcaggggacg ctggaggtcg tggtcttccc gcggacgtac
      121 gagcagacca tcgggacctg gcgcgagggc gcgatcctgc tcgtcgcggg acgggtcgac
      181 caccggggcg aggaggcgtc gctgctcgct gacgcggtgt gggactggga cacggtcgcg
      241 gaccgcgggg ccgaggcgtt cgcgcgcgag gtcggggcac tcgaccggcg gggcggcggg
      301 cggcgtggcg ggccgggcgc cccgggttcg accaatggcc atgggaacgg caacgggaac
      361 ggctacggcg cgccgcggga tcccgtcgcc gtcgggccgg gcaggggagc ggtcgtgccc
      421 gcgggggccg aagccaccgc cgtgcgcgtc tcgccgacgc gagccgccga tccgttcgat
      481 gtggctgtgg ggccgtcctc ggtcacggag gagccggacc tgccgcgcgt cgcgcccggc
      541 gagccgatcg cgacctacgc ggagccgcct gatctcgcga atgccgctat ggccggcggc
      601 gcggaggagg ccgaggagcc gccgctcccc gacgagcagc gcaacgtcgc cgccgcggcc
      661 gcccaggctc cgagcgatcc cgtcgacgcc tcaccgggcg cggtgctgaa cgtgcggttc
      721 gcgcgggacg ccgggtccga gcgggtggtc tcggcgatgc aggcgttcaa gtccgtgctc
      781 cgcgagcggc cgggtggcac gcgggtcgtc gtgcacgtgc cggcccccgg cggctcggcg
      841 ctgccgatgg agctgcgggg cgtcgcctac gacgccgagc tgctggccga gctgcgtcgg
      901 cgggtcggcg agggggtcat cgacctcagc ctgggctgag cccggcgcct cgggcctccg
      961 ggccctaccg gccgctgccg cagccggcga ggcgccgtcg aggttcaggc ctcgtcgacc
     1021 gggccatgcg tgttccagtc ggccgcacga gccgcgaccg tgagcacgat ccccatcacg
     1081 acgaaggtca tcgcgatgcc cacgccgtcc gcctggttcc gcgaaccggt ggccgcgacg
     1141 atcaggccga ccacgccggc gacgacattg aaggcaccga gcccgatccc gagcgaggcg
     1201 gcttggcggc tgccgaacgc catcccgaac ccggccacga ggtgggccgc ggcgatcacg
     1261 ccgaacagcg caaacagcgg ggtcagggcg cggatggtgg cgacgtcacc ggcgtcggcg
     1321 acgacgatgc cgcgggcgat gatcgcgctg gcgacgctca ggccgagcac ggccgaggcg
     1381 agcacgacgc ccgtcgtgat gaacaggaag ccggcggtca ggcgcgaggc cacgccacgc
     1441 tgcggctggg tcgtcgtcca gaaggtcgag gacgcggtag gggtcgaagc ggacatcgtg
     1501 tgcggctctc ccatgacccg gtcactcgac cggc
//
LOCUS       DBNHXO010000384         1921 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2888361, whole genome shotgun sequence.
ACCESSION   DBNHXO010000384 DBNHXO010000000
VERSION     DBNHXO010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2888361"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /gene="dapA"
                     /locus_tag="AC162A_07870"
     CDS             complement(<1..983)
                     /gene="dapA"
                     /locus_tag="AC162A_07870"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR5010796.1"
                     /translation="MTRTTDTTPALRGAFTALVTPFTAAGDVDEAAFRALAEWQVLAG
                     IDGLVPVGTTGEAPTLSATERDRLIEIAVATAAASPSPTRPLVVAGTGTNDTRASIDA
                     TRRAAALGADAALVVAPYYNRPDQRMLDAHFRAIADEGGLPVVVYNVPSRTGTNVEAD
                     TFLRLAEHPRVVAVKEASGNIEQIAVICRDRPRDVAVLAGDDAATLAVLAMGGDGVVS
                     VAGNEIPGEMAALCAAAHTGDWATARRIHDRWLPLYRANFAGAPNPVPAKAALVAMGL
                     LERDDLRAPLLPLTDGPRDRLVDLLRTLGLVDGGGRQDVGRRTPTGRRAAAA"
     gene            complement(1013..1765)
                     /locus_tag="AC162A_07875"
     CDS             complement(1013..1765)
                     /locus_tag="AC162A_07875"
                     /inference="COORDINATES: protein motif:HMM:NF017024.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5010797.1"
                     /translation="MQLLIYGPGRLGRAIAASAVDAGWPAADVVGRPDASGHRPPVLA
                     DVVIDASSGSAVAANVAHALAAGNRAFVLATSGWDADTSLVRSILVDHGATAVVAPNL
                     SIGAALFLRLAETAAGWYARAGSFEPSIVEWHRRGKADRPSGTARALARRIVAVDPRW
                     TVDDADDADRPALEIAGIRAGAAPGTHLVTFDGAGESIELRLAARDRSAYAEGALAAA
                     RWLRREPRTAGLHPFDEVVDDLLTPTPIPVPA"
BASE COUNT          237 a          668 c          771 g          245 t
ORIGIN      
        1 gccgctgcgg cgcgacgccc ggtgggcgtc cggcgcccga cgtcctgccg cccaccgcca
       61 tccacgaggc cgagggtccg cagcaggtcg acgagcctgt cgcgcggtcc gtcggtgagc
      121 gggagcaggg gcgctcgcag gtcgtcgcgc tcgagcaggc ccatcgccac gagggcggcc
      181 ttcgccggca cggggttcgg ggcgccagcg aagttcgcgc ggtacagcgg cagccagcga
      241 tcgtggatcc ttcgtgcggt cgcccagtcg ccggtgtgag ccgcggcgca aagtgccgcc
      301 atctcgcccg ggatctcgtt gccggccacg gatacgaccc cgtcgccgcc catcgcgagg
      361 accgccaggg tcgcggcgtc gtcgccggcg agcacggcca cgtcgcgtgg ccggtcgcgg
      421 cagatcaccg cgatctgctc gatgttgccg ctcgcctcct tcaccgcaac cacgcgcggg
      481 tgctcggcga gccgcaggaa cgtgtcggcc tcgacgttgg tccccgtgcg ggaaggcacg
      541 ttgtacacga cgacgggcag gccgccctcg tcggcgatcg cgcggaagtg ggcgtcgagc
      601 atccgctggt cgggccggtt gtagtacggg gcgacgacca gcgccgcatc ggcgccaagg
      661 gcggcggccc ggcgggtcgc atcgatcgac gcccgggtgt cgttggtgcc ggtgccggcg
      721 acgaccaggg ggcgggtcgg cgaagggctg gcggcggcgg ttgcgaccgc gatctcgatg
      781 agccggtcgc gctcggtggc cgagagcgtt ggtgcctcgc cggtcgtgcc gacgggcacc
      841 agcccgtcga tgccggccag gacctgccac tcggcgaggg cccggaatgc ggcttcgtcg
      901 acgtcgcccg ccgccgtgaa cggggtgacg agggcggtga acgcgccgcg cagggcgggc
      961 gtggtgtcgg tcgtgcgggt catgtcggtt ctccttcggg tgatgtgggg tttcaggcgg
     1021 gaacggggat cggcgtgggc gtgaggaggt cgtcgaccac ctcgtcgaat gggtgcaggc
     1081 cggcggtccg gggctcgcgg cgcagccagc gggcggcggc gagggcgccc tcggcatacg
     1141 ccgaccggtc gcgggcagcg agccggagct cgatcgattc gcccgcgccg tcgaaggtca
     1201 cgaggtgggt gcccggggcg gcgccggcac ggatcccggc gatctcgaga gccgggcggt
     1261 ccgcgtcgtc ggcgtcgtcg actgtccacc gcgggtcgac cgccacgatc cgccgggcga
     1321 gggcgcgggc ggtgccggac ggccggtccg ccttgccgcg ccggtgccac tcgacgatcg
     1381 acggctcgaa cgaaccggcc ctcgcgtacc agccggcggc ggtctcggcg agccgcagga
     1441 acaacgcggc gccgatcgag aggttggggg caacgaccgc ggtcgcgccg tggtcgacga
     1501 ggatcgagcg gaccaggctg gtgtccgcgt cccagcccga ggtcgcgagg acgaacgccc
     1561 ggttgccggc ggccagcgcg tgggcgacgt tggcggcgac ggccgagccg gaggacgcgt
     1621 cgatcacgac gtcggcgagg acgggcgggc gatggcccga cgcatctggc cgcccgacga
     1681 cgtctgcggc gggccagccc gcgtcgacgg cggaggccgc gatcgcacgg cccaaccggc
     1741 ccgggccgta gatcaggagc tgcatcttcg aacgtcctcc gcgtggagcg cggttcagga
     1801 ggtccgagaa tcacaaaggc cgcggcacca cctggtgtcc gcggcccctc ggcgcctcca
     1861 gtcccggagg ctccgtggtt cgtcgcttgc gcgccgtcag agccccaggg cccgagcctt
     1921 c
//
LOCUS       DBNHXO010000385         2359 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5469664, whole genome shotgun sequence.
ACCESSION   DBNHXO010000385 DBNHXO010000000
VERSION     DBNHXO010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5469664"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            378..1484
                     /locus_tag="AC162A_07880"
     CDS             378..1484
                     /locus_tag="AC162A_07880"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR5010798.1"
                     /translation="MAEPPIPLPDLPPGVDTPGLVVFVDRVVANIERLQRDLDARRIA
                     LRPHVKTHKSVRIARMQLEAGAAGLTVGTLGEAEVLASAGLRDLFLAYPLWAEGPKAA
                     RLRALLELADLAVGVDSIGGAERLGAAVAGVRQPLRVLIEVDSGGHRTGAASPGAAVQ
                     VAIAAARAGLRVDGVFTHGGHGYRPGGAGAAGLDEVASLEGAAAALESAGFEVRTVSA
                     GSTPTRTLAAEGRVTEIRAGTYVLGDRQQVELGAIDGDDVAAVVASTVVSTAAGHAVL
                     DAGAKALTKDLPSFVEGYGAIPAWPGAIVERLYDYHGVVSLPGPAAPPALGSVVAVVP
                     NHICPVVDLVSTFAAVQADGRVEHWAVDARGRSG"
     gene            1508..2329
                     /locus_tag="AC162A_07885"
     CDS             1508..2329
                     /locus_tag="AC162A_07885"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019067970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR5010799.1"
                     /translation="MPDPIAPAATPPLPPRADLRRRVLAGETTIGAFVNLGSVVAAEL
                     VARAGFDWALIDLEHGMGSEADLHAQLLAVQGTPTAGVVRVASAERLRVGRTLDLGAD
                     GLMIPRLETAAEIAETLTWMRYPPAGIRGVALGTRGGGYGGITHGDLAATVNDRVLGV
                     FQVESAAAVEASPETAGIDGVDVLFVGPADLSHSMGMPGRFDEPAFVAALDRVVTAAR
                     AHGKSAGILLRDASGVAAAHAQGFTFIGIGSDSSFMVAAARAGLAQARAAAGLPG"
BASE COUNT          270 a          880 c          883 g          326 t
ORIGIN      
        1 tgccgcgatg gccggactct agtccccgcc gggacccctg agaatccgaa agccgccggg
       61 tgggcccccg gcggctcggt ggtggtgcct cggttgtggc ggttctacca gcgcttggcg
      121 ccctgcacca tgaacgcgaa cgccgcgacc gtgctgagga acatcgtggc gcggtacccg
      181 taccgcttca gcgtcgactt catgagaccc ctcgatggtc cgacgaggat gggcacctcg
      241 accggcggag caggaacgta cgcccgtggc tccgggagag gtatggccca taggtaccgg
      301 taccccaccg ccttaaggtg gagttgccgg tgcgcacccc acccccgttc gggggacgcc
      361 tgcgtatgct ctgtcgcgtg gccgagccgc cgattccgct cccggacctg ccccccggcg
      421 tcgacacgcc cggactggtc gtgttcgtgg accgcgtggt ggccaacatc gagcgcctcc
      481 agcgagacct cgacgcccgc cggatcgcgc tccggccgca cgtcaagacc cacaagagcg
      541 tccgaatcgc ccggatgcag ctcgaggccg gcgcggcggg gctgaccgtg ggcacgctgg
      601 gcgaggccga ggtgctcgcg tcggcgggcc tccgcgacct gttcctcgcc tatccgctgt
      661 gggccgaagg ccccaaggcg gcccggctcc gggccctcct cgagctggcc gacctcgcgg
      721 tcggcgtcga ctccatcggc ggcgccgagc ggctcggggc cgccgtcgcc ggcgtgcggc
      781 agccgctccg ggtcctgatc gaggtggact ccgggggcca ccgcacgggg gcggcgtcac
      841 cgggggcggc cgtccaggtg gccatcgccg ccgcacgcgc gggcctccgg gtcgacggcg
      901 tgttcacgca cggcggtcac gggtaccggc cgggcggtgc cggcgcggcc gggctcgacg
      961 aggtcgcgtc gctcgagggc gcggcggccg cgctcgagtc ggcgggcttc gaggtccgca
     1021 cggtgagcgc gggctcgacg ccgacgcgca cgctcgcggc cgagggacgc gtgacggaga
     1081 tccgcgccgg gacgtacgtg ctcggcgatc gccagcaggt cgagctgggc gcgatcgacg
     1141 gcgacgacgt ggcggccgtg gtggcctcga cggtcgtctc gaccgcggcg ggtcacgcgg
     1201 tcctcgacgc cggcgccaag gcgctgacca aggacctgcc ctcgttcgtc gaaggctacg
     1261 gcgcaatccc ggcctggcct ggcgcgatcg tcgagcggct gtacgactat cacggcgtcg
     1321 tgtcgctgcc cggacccgcc gcgccgccgg cgctcgggtc ggtggtcgcg gtcgtcccga
     1381 accacatctg cccggtcgtc gacctcgtgt ccacgttcgc cgcggtacag gcggacgggc
     1441 gcgtcgagca ctgggcggtc gacgcccgcg ggcgcagcgg ctgaccgcga tcgggcatca
     1501 tcgatccatg cccgatccca tcgcccccgc cgcgactccg cccctcccgc cgcgcgcgga
     1561 cctgcgccgc cgcgtcctcg ccggcgaaac cacgatcggc gcgttcgtga acctgggctc
     1621 ggtcgtggcc gcggagctgg tcgcccgcgc gggatttgac tgggcgctca tcgacctcga
     1681 gcacggcatg ggctccgagg ccgacctgca tgcccagctg ctcgccgtcc aggggacgcc
     1741 gaccgccggg gtcgtccggg tcgcgtcggc cgagcggctg cgcgtcgggc gcacgctcga
     1801 cctcggggcc gacgggctga tgatcccgcg cctcgagacc gccgccgaga tcgcggagac
     1861 gctcacctgg atgcgctatc cgcctgccgg gatccgcggc gtggcgctcg ggacacgcgg
     1921 cggtggctac ggcgggatca cccacggcga cctcgccgcg acggtcaacg accgtgtcct
     1981 cggcgtgttc caggtggagt ccgccgcggc ggtcgaggca tcgcccgaga ccgcggggat
     2041 cgacggcgtc gacgtgctgt tcgtggggcc tgcggacctg tcgcactcga tgggcatgcc
     2101 cggccgattc gacgagccgg cgttcgtggc cgccctcgat cgggtcgtca ccgccgcccg
     2161 tgcccacggg aagtcggccg ggatcctgtt gcgcgacgcg tccggcgtcg ccgcggccca
     2221 cgcccagggc ttcacgttca tagggatcgg gtccgactcg tcgttcatgg tcgcggcggc
     2281 gcgcgccggc ctcgcccagg cgcgggcggc ggccgggctg ccgggctagc cttcccggtc
     2341 agtcctccgc cagccggac
//
LOCUS       DBNHXO010000386         2078 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3087098, whole genome shotgun sequence.
ACCESSION   DBNHXO010000386 DBNHXO010000000
VERSION     DBNHXO010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3087098"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="AC162A_07890"
     CDS             <1..378
                     /locus_tag="AC162A_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR5010800.1"
                     /translation="DLGVRIAVDDFGTGYSSLGYLRRFNVDILKIAREFIAPPESGPD
                     GWAFANAIVALGRSLGLRIIAEGIEDADQLEQLREMGCEFGQGYLFARAMPGEDMAER
                     LQPPMSRRTTGRAASQAWTTQPA"
     gene            378..938
                     /locus_tag="AC162A_07895"
     CDS             378..938
                     /locus_tag="AC162A_07895"
                     /inference="COORDINATES: protein motif:HMM:NF036410.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HIR5010801.1"
                     /translation="MFLLYAVVIGLGLGLLTGGRISGLAAIRIRWPAAIVGGLVIQVV
                     LFSPQVSSQVGDLGPWIYVASTMLVGAAVLRNWALPGMPVVAAGAACNLAAILANGGS
                     MPASRAAIELMGGQGPAIQAGYSNSSVAADPALWFLTDVFAMPRWVPFANVFSLGDLL
                     IAGGIVLVIVLAMRSGKGVTGAVGAG"
     regulatory      1061..1139
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          290 a          719 c          757 g          312 t
ORIGIN      
        1 gacctgggcg tccggatcgc ggtcgacgac ttcgggaccg gctactcgtc gctcggctac
       61 ctccgtcggt tcaacgtcga catcctcaag atcgcgcgcg agttcatcgc cccgcccgag
      121 tccggaccgg acggctgggc gttcgcgaat gcgatcgtgg cgctcgggcg atcgctgggc
      181 ctgcggatca tcgccgaggg gatcgaggac gccgaccagc tcgagcagct gcgggagatg
      241 ggctgcgagt tcgggcaggg ctacctgttc gcgcgggcga tgccgggcga ggacatggcc
      301 gagcgcctgc agccgccgat gtcgcgccgg acgaccggcc gtgccgcgtc ccaggcctgg
      361 accacccagc ccgcctgatg ttcctgctgt acgcggtcgt catcggcctc ggcctcggcc
      421 tgctcaccgg cggacgaatc tcgggcctgg ccgccatcag gatccgctgg ccggccgcga
      481 tcgtcggcgg cctcgtcatc caggtggtgc tgttctcgcc ccaggtctcg tcccaggtgg
      541 gcgacctcgg gccgtggatc tatgtcgcct cgacgatgct ggtcggcgcg gccgtcctgc
      601 gcaactgggc gctgccgggt atgccggtcg tggccgcggg cgccgcgtgc aacctggcgg
      661 cgatcctcgc caacggcggg tcgatgccgg cgagccgcgc ggccatcgag ctcatgggtg
      721 gccagggccc cgcgatccag gcgggctact ccaacagctc cgtcgccgcg gacccggccc
      781 tgtggttcct gaccgacgtc ttcgcgatgc cccgatgggt gccgttcgcg aacgtgttca
      841 gcctgggtga cctgctgatc gccggcggga tcgtgctcgt gatcgtcctc gcgatgcggt
      901 cgggcaaggg cgtgacggga gcggtcgggg ccggctgagc gcccccggtg catcgcggaa
      961 actccctcca acggacgatc gggctagtac cactgggcag tcgtcccggt acccgatcgg
     1021 cctagcctcg ggagtgaggg ctgaaaccct ccatcggggc cgccgtccag ggcaaacccg
     1081 tcgcgagacg gggacgcaaa gccagggatc ttcctcgcga agaccgcccg gttgccggag
     1141 tccggatgca tcgagacagg ctcgaaggag tcgaacgtga aggcgaacct ggcgcgcttc
     1201 acctgggccg tggcgctgct cgcgtccatg gctctggcaa tcggcgcggg catccggtgg
     1261 gagtggtggt agagatggcg tcgaacggga cgctcccctt cgccgtcatc gtcacccagc
     1321 cggcacgccc tcgcccggac atcgtcatcg aggccgggca gcgctggacc tgattggcgg
     1381 gcatatcgcc ctcagcccat cgcgaggaga ccgccggtga ggcggtctca tcgtttcaac
     1441 accggatcgg gatgcgccgc cgctccgcta cgggacgatg gtcccgccgg cggatggttg
     1501 cgcgaacgcc gtcgtcacga ctagactgcg cggacccgcc attggcgggc cactgggaga
     1561 gtgtcgcttg ggcctgttcg aggatcgcca tcgcagcccc gtcgccgtcg tcgtgacggc
     1621 ggatggccgc cgcgtcagcg tcccggtccg cgatctcacg accagcgacg tatcccttct
     1681 cgatcagctc cgctcgctgc ttgccggcct tgacggcctc cttggcctcc tcattaccgg
     1741 ctcccggcgg gagaacggag gaacgcggag ggacgagcgc cactgaggcg ccggccaacc
     1801 gaagaccgaa gggaccctcg ccgggcggcg agggtctttg catgttcggg aggtcgcggg
     1861 gccacccgga cggagaggga acccagcgga tgacgatgac gaaaggcgag cccccgacag
     1921 gcgggcctgc cgacgcgccc gggaggcgca gccacgtgcc ggccgacgga tcggcgggcg
     1981 cggcggcggt cgagcaggcg cgcaaggaca tgctccggga cgtgcgtccc cgcaagcgca
     2041 cgcggatcgg cgccgacgcg ctgatggagg cgctcatc
//
LOCUS       DBNHXO010000387         4073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4674902, whole genome shotgun sequence.
ACCESSION   DBNHXO010000387 DBNHXO010000000
VERSION     DBNHXO010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4674902"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="AC162A_07900"
     CDS             <1..254
                     /locus_tag="AC162A_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HIR5010802.1"
                     /translation="IIHEALGSTRRMLSEAEIVKRRQFRRSLVTRTALPAGHILAEAD
                     LDAKRPGTGISPDELRYTVGRELATDVAEDQVLRWEHLR"
     gene            282..1679
                     /locus_tag="AC162A_07905"
     CDS             282..1679
                     /locus_tag="AC162A_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010803.1"
                     /translation="MARPVYVVHCVDTEGPLHESVEATFERLKAIFGLDLEPSVALLR
                     QLQAGEVDLGGIEQAVRKVVDPHLLAYNDTWDKVNEMLASCMSPAFREATVDSEDNGW
                     VYNWFCVDHVDYDLNPRRRDIGYHNVFDHYQGAIRETGSAQDGVQFHYHPHNFRREAN
                     ISATHWWASSDSLTQILCRRVIDRRWFPAAHRAGFHVIRPDSHWFLEQHIPFDLSSQA
                     VAAVADDSAQAGLDGGRWGDWRRAPVTWEPYHPAHDDYQERGSCRRWVARCLNIGTRY
                     RLLSEEHVRQAFAEAEADRPVVLAFTNHDFRDMRPDIDGVRELLVRVGREFPDVPFVF
                     SEALTAMREALQLPAQPACELEVTLSRTGPSTHALEIRSQVPTFGPQPWLALRTVAGT
                     YHHDNLDIDEPFHRWRYVLDEETYPLGALDAIGLAANNAYGTTTVVNVDPATGAASRR
                     VLNDPSAAAVAGHGS"
     gene            1682..2743
                     /locus_tag="AC162A_07910"
     CDS             1682..2743
                     /locus_tag="AC162A_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR5010804.1"
                     /translation="MDPQRPEMLEAVVAPPELSITDAIAQLDMAGTGGLVLCRPDQQV
                     AGLLTDGDIRRAVLHKVSLADPCGSIATPDPVVARAPIEPAEALQLMLEHDINHLPVV
                     DEAGVLVDFLLRKNLVADVPGVMSAVVMAGGFGKRLLPLTENVPKPMLPVGERPLLER
                     TIDQLRGAGISEVHLTTHYLPESIIGHFGDGEAFGVHISYAAEDEPMGTAGGLRLVPR
                     PNGPFLVINGDILTRVSYQGMLRFHRKHAATLTVGIRAHEVEVPFGVVECDDVRVMDL
                     TEKPRIRLLVNAGIYLLEPGAWDFIPGGRRFDMTDLIRRLLDEGQVVVGYPIIEYWQD
                     VGRHEDYNRAQEDVRDGRF"
     gene            2754..4067
                     /locus_tag="AC162A_07915"
     CDS             2754..4067
                     /locus_tag="AC162A_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HIR5010805.1"
                     /translation="MDPGDEADATESPGAAMHALARRLFPITRSITGDGVRETLRILG
                     EHLPLEIHEVPTGTRAFDWTVPPEWRIRDAYILDEHGDRIVDFQRNNLHVVGYSVPVD
                     ATMSLAELQPHLHSLVDQPTAIPYVTSYYERRWGFCLTHAQRQSLQDGLYRVVIDSEL
                     KDGSLTYGELVIPGASPSEVFLSTYVCHPSMANNELSGPVVTTWLAKWIASRPRHYTY
                     RLVFIPETIGSLVYLSRHVDTLRANVVAGFNVTCVGDDRAYSFLPSRNGGTLADRVAL
                     HTLKTRHPEFVRYSFLDRGSDERQYCSPGVDLPVVSVMRTKYHDYPEYHTSLDDLTVV
                     TPAGLEGGYTILRDCLEVLEGNRTYLSTCLGEPHLGGRGLYPTLGTASSHGAVRTTMD
                     LLAYADGTHDLVEIGDIIGVPVMDLRRVAEQLVELGLLTVVPGPR"
BASE COUNT          643 a         1525 c         1283 g          622 t
ORIGIN      
        1 gcatcatcca cgaggcgctg ggcagcacgc gccggatgct gtcggaggcc gagatcgtca
       61 agcgccgcca gttccggcgc agcctggtca cgcggaccgc gctgccggcc ggtcacatcc
      121 tcgccgaggc ggacctggac gcgaagcgcc cgggaaccgg catcagcccc gacgagctcc
      181 gctacacggt cggccgcgag ctggccacgg acgtcgccga ggaccaggtg ctccgctggg
      241 agcacctgcg ctagatttca cgcactcctc ctgaggatcc aatggcacgc cccgtctacg
      301 tcgtccactg cgtggacacg gaaggaccgc tccacgaatc ggtcgaggcg accttcgaac
      361 gcctcaaggc catcttcggg ctcgatctcg agcccagcgt cgcactgctc cggcagctgc
      421 aggcgggcga ggtcgacctc ggcggcatcg agcaggccgt gcgcaaggtc gtggaccccc
      481 acctgctggc ctacaacgac acctgggaca aagtcaacga gatgctcgcg tcgtgcatgt
      541 cgccggcgtt ccgggaggcg accgtcgatt ccgaagacaa cggctgggtc tacaactggt
      601 tctgcgtcga tcacgtggac tacgacctca acccgcggcg tcgcgacatt ggctaccaca
      661 acgtcttcga ccactaccag ggcgcgatcc gcgagaccgg ctccgcccag gacggcgtcc
      721 agttccacta ccacccccac aacttccgcc gcgaagccaa tatcagcgcg acgcactggt
      781 gggcgtcctc cgacagcctg acccagatcc tctgtcgccg ggtcatcgat cgccgctggt
      841 tcccggccgc gcatcgagct gggttccacg tcatccggcc ggacagccac tggttcctgg
      901 agcagcacat cccgttcgac ctctccagcc aggccgtcgc cgccgttgcc gacgactccg
      961 cccaagcggg cctggacggc ggccgctggg gcgactggcg ccgagccccc gtcacctggg
     1021 agccctacca cccggcccac gacgactacc aggagcgcgg gtcgtgccgc cgctgggtcg
     1081 cccgctgtct caacatcggg acccgctacc gcctgctgag cgaggagcac gtgcggcagg
     1141 cgttcgccga ggcggaggcg gaccggccgg tcgtcctcgc cttcaccaac cacgacttcc
     1201 gcgacatgcg ccccgacatc gacggcgtgc gggagctcct cgtccgggtg ggacgcgagt
     1261 tcccggacgt tccgttcgtg ttcagcgaag ccctcaccgc aatgcgcgag gcgctgcagc
     1321 ttccggctca acccgcctgc gagctcgagg tcacgctctc ccggacaggc ccatccaccc
     1381 acgccctcga gatccggtcc caggtcccga ccttcgggcc ccagccgtgg ctggccctgc
     1441 gcacggtcgc cggcacctat caccacgaca acctcgacat cgacgagccg ttccaccgct
     1501 ggcgctacgt cctcgacgag gagacctacc cgctcggtgc gctcgacgcc atcggcctcg
     1561 cggccaacaa cgcctacggc acgacgaccg tcgtgaacgt cgatcccgcc actggcgccg
     1621 cgagtcgccg cgtcctcaac gacccgtcgg ccgccgccgt ggccggccac gggtcttgat
     1681 cgtggacccc cagcgcccgg agatgctcga ggccgtcgtc gcgccgccgg agctgtccat
     1741 caccgacgcc atcgcacagc tggacatggc agggaccggc ggcctcgtcc tgtgccgtcc
     1801 ggatcagcag gtcgccggac tcctcaccga cggtgacatt cgccgcgcgg tcctgcacaa
     1861 ggtgtcgctg gccgacccat gcggctccat cgccaccccg gaccccgtcg tcgcgcgcgc
     1921 gcccatcgaa ccggccgagg cgctccagct gatgctggag cacgacatca accacctccc
     1981 ggtcgtcgac gaagccggtg tcctggtcga cttcctgctc cggaagaacc tcgtcgccga
     2041 tgtgcctggc gtcatgtctg ccgtggtcat ggccggcggc ttcggcaagc gcctgttgcc
     2101 gctgacggag aacgtcccca agcccatgct gccggtgggc gagcggccgc tcctggaacg
     2161 cacgatcgac cagctgcgcg gggccgggat cagcgaggtc cacctcacca cccactatct
     2221 gcccgaaagc attatcggcc acttcggcga cggcgaggca ttcggggtcc acatcagcta
     2281 cgccgccgag gatgagccga tgggcacggc gggcggcctc cggttggtcc cgcgtccgaa
     2341 tgggccgttc ttggtgatca acggcgacat cctgacccgg gtctcctacc agggcatgct
     2401 tcgcttccac cggaagcacg ccgcgacgct gacggtgggc atccgcgccc atgaggtcga
     2461 ggtcccattc ggggtcgtgg agtgcgacga cgtccgcgtg atggacctca ccgagaagcc
     2521 gcgcatccgg ctgctcgtga acgccggcat ctacctgctg gagcccggcg cctgggactt
     2581 catcccgggc ggccggcgct tcgacatgac cgacctcatt cggcgcctgc tggacgaggg
     2641 ccaggtcgtg gtgggctacc cgatcatcga gtattggcag gatgtcggcc gtcacgagga
     2701 ctacaaccgc gcccaggaag acgtgcgaga tggacggttc tgacgggcct cggatggatc
     2761 ccggcgacga ggcggacgcg acggaatcgc ccggcgcggc gatgcacgcg ctcgcgcgac
     2821 gcctgttccc gatcacccgg agcatcaccg gggacggtgt gcgcgagacg ctccgcatcc
     2881 tcggcgagca cctccccctg gagatccacg aagtgcccac cggcacccgt gccttcgact
     2941 ggaccgtgcc ccccgagtgg cgcatccggg atgcctacat cctcgacgag cacggcgacc
     3001 ggatcgtcga cttccagcgg aacaacctgc atgtcgtcgg ctactcggtc cccgtcgacg
     3061 ccaccatgtc gctggccgag ctccagccgc acctccactc gctggtcgac cagcccaccg
     3121 cgatcccgta cgtcacctcc tactacgaac ggcggtgggg cttctgcctg acgcacgccc
     3181 agcggcagag cctgcaggac ggcctgtacc gggtcgtgat cgacagcgag ctcaaggacg
     3241 gctccctcac ctacggcgag ctcgtcattc ccggcgcctc gcccagcgag gtgttcctgt
     3301 ccacctacgt ctgccatccg tcgatggcca acaacgagct ctccggcccg gtggtcacga
     3361 cctggctcgc caagtggatc gccagccggc ctcgccacta cacctaccgc ctggtcttca
     3421 tccccgagac catcggatcg ctggtctacc tgtcacgtca cgtcgacacg ctcagggcca
     3481 acgtggtggc gggcttcaac gtgacctgtg tcggcgatga ccgggcgtac tccttcctgc
     3541 cttccaggaa cggtggcacg ctcgcggacc gagtcgccct ccacaccctc aagaccaggc
     3601 atccggagtt cgtccgctac tcgttcctcg accgcggcag cgacgagcgg cagtactgca
     3661 gcccgggcgt cgaccttccc gtcgtgagcg tgatgcgcac caagtatcac gactatcccg
     3721 agtaccacac gtcgctggac gacctgacgg tcgttacgcc cgccgggctc gagggcggct
     3781 acaccatcct gcgcgactgc ctggaggtcc tggagggtaa ccggacgtac ctgtcgacct
     3841 gcctcggcga gccccacctt ggcggccggg gcctctatcc gacgctcggc accgcatcca
     3901 gtcacggagc ggttcggacg accatggacc tcctggcgta cgcggacgga acgcacgacc
     3961 tcgtcgagat cggcgacatc atcggggtgc cggtcatgga cctccggcgt gtggccgagc
     4021 agctcgtgga gctcgggttg ctgaccgtgg tgccggggcc gcgctgacgg gcg
//
LOCUS       DBNHXO010000388         4174 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_707282, whole genome shotgun sequence.
ACCESSION   DBNHXO010000388 DBNHXO010000000
VERSION     DBNHXO010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_707282"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="AC162A_07920"
     CDS             complement(<1..475)
                     /locus_tag="AC162A_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010806.1"
                     /translation="MGFTLKRFFAPKATVRWPEQPADVAPKFRGRLQLLYDEYGTLKC
                     ETCFQCAQACPIECIDMGGIDTKGRFHVHWGPPETYGERREESALRRSGRTVPDAAFA
                     RFDPIDTTALDEILDRHDYDPRALLTILEETQAEYGYLPVGALKHISRITGTWYAM"
     gene            complement(508..1854)
                     /locus_tag="AC162A_07925"
     CDS             complement(508..1854)
                     /locus_tag="AC162A_07925"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012373.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1/NuoH family protein"
                     /protein_id="HIR5010807.1"
                     /translation="MNRAFSNLTPVGKILLPLLFLVLVVGPILTIILAVYIGPWLLGI
                     LNDNIEIVRFVVSVTAILLFSIPAAFIIIYMEMKVIALMNLRLGPDRVGPAGSLLSVV
                     HGLKVLMKEDFTPTGADSIVFTWAPVVVFLTAVMTFLVIPFGPGLVGQDLNIGLLYLF
                     AVGGMTVVGLLMAGWSSFNKYSLVGGLRSAAQVVSYEIPLTLSVVGLILLAGTMSVNQ
                     IGVQQAGWFTDWFVFRQPLGALIFFIAATAEANRTPFDLTEADSEIVAGFATEYSGMR
                     FGFLFFAEYVNVFIISALMVTLYFGAWNAPFAFPEIQLALDPGQLGNNLVVLLATAPV
                     IFTLAFAAPFFLAQSSRPAWQALLLGFLTFNVVIIGLLFGVTYLGLDWVAGLAWFLLK
                     TFLFVYLFVWMRGTLPRVRIDQLMGFAWKWLLPAALLNLFLTAAAIVVVDGLRGGL"
     gene            complement(2057..3298)
                     /locus_tag="AC162A_07930"
     CDS             complement(2057..3298)
                     /locus_tag="AC162A_07930"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HIR5010808.1"
                     /translation="MTAPAAPHQHPSADDPRVMLDDEIGHFEPVPPYPPRTEREAEFY
                     TLGDGEMFLNMGPQHPSTHGVLRVVLKISGERVVDLDPVLGYLHRGVEKLCENADYHQ
                     VISQVDPLEYVSSLFCEWSPVMAFEHLLDVEVPRRAEYIRVLTGELNRIASHALFMGW
                     MALDLGGLTPILYSFIERDEICEMLASVTGQRMLFNYFRIGGVNGDINHEFMSRLGDW
                     MSRAAEQHDANTQLLNENEIFVRRMRDIGRLDSATALRMAVTGPNLRATGIPFDIRRA
                     HPYSVYPEIEFDVPTRGHGPEGGDALDRYLLRVEEVKQSLRIIDQCLHNMPDGPVMAK
                     LPRLLRPRPGRAWAGIEGPRGLYGTYAISDGTDQPFRMKIHDPSFVHLQLVGMLMRDN
                     LIADTMAVMASLDPIMGGVDK"
     gene            complement(3301..3849)
                     /locus_tag="AC162A_07935"
     CDS             complement(3301..3849)
                     /locus_tag="AC162A_07935"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012549.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR5010809.1"
                     /translation="MDRLLRARLEATLGDRLTGPVRQRHDQTEIRVSPADLIEVMRSL
                     HDDPSLGFEILADLAGVDTGSHMQVVYHLWSAQTPDWLRVIADGVPRDDPRVPSLTGL
                     WPGVEWMEREAYDMFGIIFEGNRDLRRIYMPPDYTSFPLRKDFHLPDDAARSAGGGVR
                     HVEQGHMPAQATPSVARRTVRA"
     gene            complement(3852..>4174)
                     /locus_tag="AC162A_07940"
     CDS             complement(3852..>4174)
                     /locus_tag="AC162A_07940"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR5010810.1"
                     /translation="QADVLIVAGTLTTKMAGPLVRLWEQMPEPKWCVAMGDCTCSGGR
                     YKRSYSTIEGIDRVMPVDVYVPGCPPRPEGLIYGMMKLQQLVKDRRGEWHERPIGPTV
                     PQDV"
BASE COUNT          706 a         1381 c         1478 g          609 t
ORIGIN      
        1 tcatcgcgta ccaggtgccg gtgatccgcg agatgtgctt gagcgcgccg accggcaggt
       61 agccgtactc cgcctgggtc tcctcgagga tcgtgagcag cgccctgggg tcgtagtcgt
      121 ggcgatccag gatctcgtcg agcgcggtcg tgtcgatcgg gtcgaagcgg gcgaaggcgg
      181 cgtcgggtac ggtgcggcca gaccggcgca gcgccgactc ctcgcgccgc tcgccgtagg
      241 tctcgggcgg gccccagtgg acgtggaacc ggcccttggt gtcgatgccg cccatgtcga
      301 tgcactcgat cgggcaggcc tgcgcgcact ggaagcaggt ctcgcacttg agcgtgccgt
      361 actcgtcgta gagcagctgg agccggcccc ggaacttggg cgcgacgtcg gcgggctgct
      421 cgggccagcg gaccgtggcc ttgggggcga agaagcgctt cagggtgaag cccatcccgc
      481 ggaccatgcc catgcccgga acgatgctca cagcccgccc cggagtccgt ccacgacgac
      541 gatcgcggcc gccgtgagga acaggttgag cagcgcggcc ggcagcagcc acttccacgc
      601 gaagcccatg agctggtcga tccggacgcg cggcagcgtg ccccgcatcc acacgaacag
      661 atacacgaac aggaacgtct tgagcaggaa ccacgccagg ccggcgaccc agtcgaggcc
      721 gaggtaggtg accccgaaca gcaggccgat gatcacgacg ttgaacgtga ggaatccgag
      781 cagcagcgcc tgccaggccg gccgcgagct ctgcgcgagg aagaacggcg ccgcgaaggc
      841 gagcgtgaag atcaccggcg ccgtggccag cagcacgacc aggttgttgc ccagctggcc
      901 cgggtctaga gcgagctgga tctccgggaa agcgaacggc gcgttccagg ccccgaagta
      961 cagggtcacc atcagcgccg agatgatgaa cacgttcacg tactcggcga agaacaggaa
     1021 cccgaagcgc atgcccgagt actcggtcgc gaagccggcc acgatctccg agtcggcctc
     1081 ggtgaggtcg aacggggtcc ggttggcctc ggcggtggcg gcgatgaaga agatcagcgc
     1141 gcccaggggc tggcggaaca cgaaccagtc cgtgaaccag cccgcctgct gcacgccgat
     1201 ctggttgacc gacatcgtgc cggccagcag gatcaggccg acgaccgaga gcgtgagcgg
     1261 gatctcgtac gagacgacct gcgcggccga ccgcaggccg ccgaccagcg agtacttgtt
     1321 gaagctcgac cagccggcca tcagcaggcc gaccacggtc atgccgccga cggcgaacag
     1381 gtacagcagg ccgatgttga ggtcctggcc gaccagccct ggcccgaacg ggatgacgag
     1441 gaaggtcatg accgcggtca ggaacacgac caccggcgcc caggtgaaca cgatgctgtc
     1501 ggcgcccgtc ggcgtgaagt cctccttcat gaggaccttg agcccgtgca cgaccgagag
     1561 cagtgacccg gcgggcccga cgcggtcggg tccgagacga aggttcatca gcgcgatgac
     1621 cttcatctcc atgtagatga tgatgaacgc tgccgggatg ctgaacagca ggatcgccgt
     1681 gaccgagacc acgaagcgga cgatctcgat gttgtcgttg aggatcccca gcagccaggg
     1741 cccgatgtag acggcgagaa tgatcgtcag gatcggcccg acgaccaata ccaggaacag
     1801 caacggcagc aggatcttcc caacaggggt cagattgctg aacgcccgat tcatcgcggt
     1861 catcgccgcg gccctggggt gaactggcgc acggggaacg tggacaagcc cgaccaggac
     1921 gcgccgagcg caaggcgccc gcgagcattg accctccgag gtccgaggcg acgctcctgc
     1981 gagccatcgg cccgaggatt gcgtgagcga gcgcgcggag cgggcaacgc cgcgggcggc
     2041 cgtgcctggg cgggcttcac ttatccactc cgcccatgat ggggtcgagg gatgccatca
     2101 ccgccatggt gtcggcgatc aagttgtcgc gcatcagcat cccgaccagc tggaggtgca
     2161 cgaatgaggg gtcgtggatc ttcatccgga acggctgatc ggtgccgtcg gagatcgcgt
     2221 acgttccgta cagcccgcgc gggccctcga tcccggccca cgcgcgaccg ggccggggcc
     2281 gcagcagtcg cggcagcttg gccatcaccg ggccatcggg catgttgtgc aggcactggt
     2341 cgatgatccg caggctctgc ttgacctcct cgacccgcag caggtagcgg tcgagcgcgt
     2401 cgccgccctc cggtccgtgg ccacgcgtcg ggacgtcgaa ctcgatctcg gggtagaccg
     2461 agtacgggtg cgcccggcgg atgtcgaacg ggatccccgt cgcgcggagg ttcggccccg
     2521 tgaccgccat ccggagcgcc gtggcgctgt ccagccggcc gatgtcgcgc atccggcgga
     2581 cgaagatctc gttctcgttg agcagctgcg tgttcgcgtc gtgctgctcc gctgcccggc
     2641 tcatccagtc gcccaggcgg gacatgaact cgtggttgat gtcgccgttg acgccgccga
     2701 tccggaagta gttgaacagc atccgctggc ccgtcaccga ggccagcatc tcgcagatct
     2761 cgtcgcgctc gatgaagctg tacaggatcg gcgtcaggcc acccaggtcg agcgccatcc
     2821 agcccatgaa cagcgcgtgg ctggcgatcc ggttcagctc gccggtgagc acccggatgt
     2881 actcggcgcg ccgcggcacc tcgacgtcga gcaggtgctc gaacgccatc accggcgacc
     2941 actcgcagaa cagcgagctc acgtactcga gcgggtccac ctggctgatg acctggtggt
     3001 agtcggcgtt ctcgcacagc ttctcgacgc cgcggtggag gtaaccgagg acggggtcga
     3061 ggtcgacgac gcgctcgccg ctgatcttga gcacgacccg cagcacgccg tgggtcgacg
     3121 ggtgctgggg ccccatgttg aggaacatct cgccgtcgcc gagcgtgtag aactcggcct
     3181 cgcgctcggt ccgcggcggg tacggcggga cgggctcgaa gtgcccgatc tcgtcgtcga
     3241 gcatcacccg tgggtcgtcc gcggacgggt gctggtgggg cgcggcgggg gcggtcatcg
     3301 tcaggctcgc accgttcggc gggccacgga cggggtcgcc tgggcgggca tgtggccctg
     3361 ctccacgtgg cgcacgccgc caccggcgga ccgcgccgca tcgtcgggca ggtggaagtc
     3421 cttgcgcagc gggaagctcg tgtagtcggg cggcatgtag atgcgccgca ggtcgcggtt
     3481 gccttcgaag atgatcccga acatgtcgta ggcctcgcgc tccatccact cgacgcccgg
     3541 ccacaggccg gtcagcgagg gaacgcgcgg gtcgtcgcgg ggcaccccat cggcgatcac
     3601 gcgcagccag tcgggggtct gcgccgacca caggtggtag accacctgca tgtgactgcc
     3661 ggtgtcgacg ccggcgaggt ccgcgaggat ctcgaagccc agcgatgggt cgtcgtgcag
     3721 cgagcgcatg acctcgatca ggtccgcggg agacacacgg atctcggtct ggtcgtggcg
     3781 ctggcgaacg gggccggtga gccggtcgcc gagcgtcgcc tcgaggcgcg cccgcagcag
     3841 gcggtccatc gctagacgtc ctggggcacg gtcgggccga tcgggcgctc gtgccactcc
     3901 ccgcggcggt ccttgaccag ctgctggagc ttcatcatcc cgtagatcag gccctcggga
     3961 cgcggcgggc agccgggcac gtagacgtcg acgggcatga cgcggtcgat gccctcgatc
     4021 gtcgagtagc tgcgcttgta gcgaccaccc gagcacgtgc agtcgcccat cgcgacgcac
     4081 cacttgggct ccggcatctg ctcccagagt cgaacgagcg ggccggccat cttcgtggtc
     4141 agcgtgcccg cgacgatcag gacgtccgcc tgcc
//
LOCUS       DBNHXO010000389         1819 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_707770, whole genome shotgun sequence.
ACCESSION   DBNHXO010000389 DBNHXO010000000
VERSION     DBNHXO010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_707770"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..>1819
                     /locus_tag="AC162A_07945"
     CDS             155..>1819
                     /locus_tag="AC162A_07945"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5010811.1"
                     /translation="MAGRLVSPITIGRRAELEAGMLALDAAIDGSPTNLLIAGEAGVG
                     KSRFVAELAAGAEVRRMLVLRGGCANVGDEGLPYGPIVEILGQLARDLDPADLEPIVG
                     SSGADLARLVPALEPVSAAAPVQQAWLQTRLFEALMGLMRRLASASPVLMIIEDVHWA
                     DAATRETIAFLVRNLRTERVVIAMTLRSDELHRRHPALPWLAELERTGRIERIDLARL
                     DAVETAAMLEAIMGESADPEMASRIHRRADGNPFFIEELLIAEGAPGSSRLPTTLREI
                     LLARLAALGDPARDVVNMVAIVGRRIDHDLLAGVAAAEPDRLDAALREAIGAQIVVVD
                     RDSRGADGYAFRHALLAEVAYDELLPGERRRLHLAMAEALSTIPAPTGGSAAAYWAEI
                     AHHWANARQDDRAFDASIRAAAAAEDAFAFTAAAHALERVIDLWPVIPDAESRSGMDM
                     PELLGRAAQVVNLAGLPTRHVALRRAALAALPPDVAPARAAVARERLARALWAFGDSA
                     SALVEAEAAVAILDAEPPSAERARVLAGYGQMLMLLDRWTESRAACE"
BASE COUNT          224 a          685 c          665 g          245 t
ORIGIN      
        1 gtgccggagt cgcccccgcc gggccggcgc cccatcgcgc ggttcgtggc ccgggcgagg
       61 acgttcgtcc aggccggacg tcccagcagg ccgatcccgg cacaggcggc cgactgagcg
      121 cgaggcacgc gtgatcgctc ctatgatctc gcgcatggcc ggccgtcttg tcagtcccat
      181 caccatcgga cgccgcgccg agctcgaggc cgggatgctg gcgctcgacg ccgcgatcga
      241 cggatcgccg accaatctcc tgatcgcggg cgaggcgggc gtcggcaaga gccgtttcgt
      301 cgccgagctc gccgcgggcg ccgaagtgcg ccgcatgctc gtgctccggg gcgggtgcgc
      361 caacgtcggc gacgaggggc tgccgtacgg gccgatcgtc gagatcctcg gccagctcgc
      421 gcgcgacctc gatccagccg atctcgagcc gatcgtcggg tcgtcgggcg cggacctcgc
      481 ccggctcgta cccgcgctcg agccggtctc ggccgcggcg ccggtccagc aggcctggct
      541 ccagacccgc ctgttcgagg cgctcatggg cctcatgcgc cggctcgcgt ccgcatcgcc
      601 cgtcctgatg atcatcgagg acgtccactg ggcggacgcg gcgacccgcg agacgatcgc
      661 cttcctggtc cggaacctgc gcaccgagcg cgtggtcatc gccatgaccc tccggtccga
      721 cgagctccac cggcgccacc cggccctgcc gtggctggcc gagctcgagc ggacgggccg
      781 catcgagcgg atcgacctcg ccaggctgga cgcggtcgag acggcggcaa tgctcgaggc
      841 gatcatgggc gagtccgccg atcccgagat ggcgtcgcgc atccaccggc gagccgacgg
      901 gaacccgttc ttcatcgagg agctgctgat cgccgagggc gcccccggat cgtcgcgcct
      961 gccgacgacg ttgcgcgaga tcctcctcgc gcgactcgct gccctcggcg atcccgcccg
     1021 cgacgtcgtc aacatggtgg cgatcgtcgg ccggcgcatc gaccacgacc tcctggccgg
     1081 cgtcgccgca gcggagccag accgcctcga cgccgcgctg cgcgaggcga tcggcgccca
     1141 gatcgtggtc gtggatcgag actcccgcgg cgccgacggc tacgcgttcc ggcacgcgct
     1201 cctcgccgag gtcgcatacg acgagctgct gcccggcgag cgacgccggc ttcacctagc
     1261 gatggccgag gcactctcga cgatccccgc gccgacgggc gggtcggcag cggcctactg
     1321 ggcagagatc gcgcaccact gggcgaatgc ccgccaggac gaccgcgcgt tcgatgcctc
     1381 gatcagggcg gccgcggccg cggaggacgc gttcgcgttt acggccgcgg cgcatgccct
     1441 ggaacgggtc atcgacctgt ggccggtgat ccccgacgcc gagtcgcgct cgggcatgga
     1501 catgcccgag ctcctgggcc gagcggcgca ggtggtcaac ctggccgggc tgccgacccg
     1561 tcacgtggcc ctccgccgcg cggcgctcgc ggccttgccg cccgacgtcg cgccggcgcg
     1621 ggcagcggtc gcgcgcgagc ggctcgcccg cgcgctgtgg gcgttcgggg actcggcgtc
     1681 ggcgcttgtc gaggccgagg ccgctgtggc gatcctggac gcggagccgc cgagcgccga
     1741 gcgcgcccgg gtactggcgg gctacgggca gatgctgatg ctgctcgacc gttggaccga
     1801 gtcccgcgcc gcgtgcgag
//
LOCUS       DBNHXO010000390         3369 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3486827, whole genome shotgun sequence.
ACCESSION   DBNHXO010000390 DBNHXO010000000
VERSION     DBNHXO010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3486827"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="AC162A_07950"
     CDS             <1..384
                     /locus_tag="AC162A_07950"
                     /inference="COORDINATES: protein motif:HMM:NF016142.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HIR5010812.1"
                     /translation="VVSIGIDEVPGFYARGSGIKAPTSVPDVAGAAAVIRAHEALGLG
                     SGLVICNPVPEELALPDDLTRAAVDQAIREADNTGVGGAALTPWLLARVAAITDGASV
                     KANIALIVNNAKVGGELAGELVTAG"
     gene            complement(471..689)
                     /locus_tag="AC162A_07955"
     CDS             complement(471..689)
                     /locus_tag="AC162A_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010813.1"
                     /translation="MFATSAVKPFSVRIRPYARIQYKGRPGPSSPIRPRADAYRTKPG
                     ALLTFAGIAQATAVGGFGFAARPAAGAE"
     gene            970..2103
                     /locus_tag="AC162A_07960"
     CDS             970..2103
                     /locus_tag="AC162A_07960"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5010814.1"
                     /translation="MAAARPSQSLPPAISVTAVRRVYATKPKPVVALAGVDLEVAPGE
                     FFGLLGPNGAGKTTLIKILTTLLLPTSGTARIFGFDIATETKRIRRIMNMVAGGEQSG
                     YGILTVREQLWMFSQFYGLGTREGWKRTDELIEAVGLGEQRLQRVSTLSTGQRQKMNM
                     ARGLLNDPWILFLDEPTLGLDVAAARSIRELVLDWKGAVPGRTVLLTTHYMAEADELC
                     ERIAIVDHGRILAIGTPDELKKRVQRESIFRLELDGLDAGMAALSRLPGVVSAAPASD
                     ADPELQRVTVNLVLSEDAALGGVVTALANLGSHILALRKSEPTLEDVFVELVGRGFGD
                     ARDEGHGEGPGSGRDDGDEGVEDLPPAPSADEAHEAYPVEAGR"
     gene            2100..3017
                     /locus_tag="AC162A_07965"
     CDS             2100..3017
                     /locus_tag="AC162A_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5010815.1"
                     /translation="MTTDAAPVETPRASIFASSRNGTDPRTASIWTRRQVIENNARSL
                     VGRAFPRVRGMAREPSWVFFEILLPFLTTSAFVLVYRTLQAPEAYVGFVVLGGAMAAF
                     WLNVVWMMAAQLYWEKDQGNLELYFAAPMNLMSVLLGMAVGGLIMSCTRALAILVVST
                     WVFGVTFSVQQWGLLIAVFFLTLSALYGLGMAMASLYLLWGREAFHLTNVMIEPVYFV
                     SGLNFPVGKLGALGMVAIATIPFAVGLDAMRQLVFTGQPYITGTPSPEVEALILVAMT
                     VGFTLLARYLIRRIERMARARGTLSLRWQ"
     gene            3014..>3369
                     /locus_tag="AC162A_07970"
     CDS             3014..>3369
                     /locus_tag="AC162A_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010816.1"
                     /translation="MSAITGEGLAGTSPTFDLGRTEGGGALETGRNLRTALLLGWRVE
                     SNWTDPVLFFIYTVARPIASLLLLVAMVTIIGGAANEEVRTFVVLGSALWAMLVAGLS
                     GPAWSVLEDRERYRML"
BASE COUNT          476 a         1153 c         1214 g          526 t
ORIGIN      
        1 gtggtgtcca tcgggatcga cgaggtgccc gggttctacg cccgcggctc cgggatcaag
       61 gcgcccacca gcgtgccgga cgtcgccggg gcggctgccg tgatccgcgc ccacgaggcg
      121 cttggcctgg gatccggcct cgtgatctgc aacccggtcc cggaggagct agcgcttccc
      181 gacgacctga cccgggcggc cgtggaccag gcgatccgcg aggcggacaa cacgggggta
      241 ggcggcgcgg cgctcacgcc ctggctgttg gccagggtcg ccgcgatcac cgacggtgcg
      301 tcggtcaagg ccaacattgc gctgatcgtc aacaacgcca aggtcggcgg cgagctggcc
      361 ggggagctgg tgactgccgg ctagcgccgg acatgcggat gcgccggccg gcggtaaggc
      421 cggtccggcg ctggagcgcg gggcgcgcga ttgacgactg ctgagctgga ttactcggcg
      481 ccggccgcgg gccgagctgc gaacccgaag ccgccgacgg cggtcgcctg cgcgatgcct
      541 gcgaacgtga ggagcgctcc cggcttggtg cgataggcgt ccgcccgcgg gcggatgggg
      601 ctcgagggac cggggcggcc cttgtactgg atccgggcgt aggggcggat ccggacgctg
      661 aacggcttga cggcgctggt ggcgaacatc tctggtggtc tccggtcgag gtcttggtgg
      721 cgggccacga ctgggcttgg atggagcagt cgcgaagttg atgaccagag cctaggaacc
      781 cgcgccgcca ccgttgatgg acccgggtcg acaagccgat gacgacaaac gtcaatgcct
      841 cgggcggcac gcgcccgagg ccccgacctg ttcgacaacc gtaatgcgcg tgtaagatcg
      901 ccatcttcca tcaacgggtc gagccgtggt agcatccggc ttcccgcatg aggtccgcac
      961 cggctgacca tggccgccgc acgcccgagc cagagcctcc cgccggcgat ctccgtcacc
     1021 gccgttcggc gcgtctacgc gacgaagccg aagcccgtcg tcgcgctcgc cggtgtcgac
     1081 ctcgaggtcg cgcccggcga gttcttcggg ctgctcggcc cgaacggtgc gggcaagacg
     1141 acgctgatca agatcctgac cacgctcctg ctgccgacca gcgggaccgc gaggatcttc
     1201 ggcttcgaca tcgccactga gaccaagcgg atccgacgga tcatgaacat ggtcgccggg
     1261 ggcgagcagt cgggctacgg gatcctgacc gtccgtgagc agctgtggat gttcagccag
     1321 ttctatggcc tgggcacgcg cgagggctgg aagcggacgg acgagctgat cgaggccgtc
     1381 gggctgggcg agcagcgcct ccagcgggtg agcacgctgt cgaccggcca gcgccagaag
     1441 atgaacatgg cccgcggcct gctcaacgac ccgtggatcc tgttcctcga cgagccgacg
     1501 ctgggtctcg acgtcgccgc cgcccgctcg atccgcgagc tcgtcctcga ctggaagggc
     1561 gccgtcccag ggcggaccgt gctcctgacg acgcactaca tggccgaggc ggacgagctg
     1621 tgcgagcgga tcgcgatcgt cgaccacggc cggatcctgg cgatcggcac gcccgacgag
     1681 ctcaagaagc gcgtccagcg cgaatcgatc ttccgcctcg agctcgacgg actcgacgcg
     1741 ggcatggccg ccctgtcgcg gttgcccggg gtcgtctcgg cggcgccggc ctcggacgcc
     1801 gacccggagc tccagcgggt gaccgtcaac ctcgtcctgt ccgaggacgc ggcactgggc
     1861 ggtgtcgtca ccgcgctcgc caacctcggc agccacatcc tcgccctgcg caagtccgag
     1921 ccgacgctcg aggacgtgtt cgtcgaactg gtcggccgcg gcttcggcga cgcgcgtgac
     1981 gagggccacg gcgagggccc cggatcgggc cgggatgacg gggacgaggg cgtcgaggac
     2041 ctgccgcccg cgcccagcgc cgacgaggcg cacgaggcct acccggtgga ggccggccga
     2101 tgacgacgga cgcggcgccg gtcgagacgc cgcgggcgtc gatcttcgcc agctcgcgca
     2161 acggcacgga cccgcgcacg gcctcgatct ggacccgccg ccaggtgatc gagaacaacg
     2221 cgcgttcgct cgtcggccgc gccttcccgc gcgtccgggg aatggcgcgc gagccctcct
     2281 gggtgttctt cgagatcctg ctgccgttcc tgaccacgtc ggcgttcgtg ctcgtctatc
     2341 gcaccctgca ggcccccgag gcctacgtcg gcttcgtcgt cctcggcggc gccatggcgg
     2401 cgttctggct caacgtcgtg tggatgatgg cggcccagct gtactgggag aaggaccagg
     2461 ggaacctcga gctgtacttc gccgcgccga tgaacctgat gtcggtgctg ctcgggatgg
     2521 ccgtcggcgg cctgatcatg agttgcacgc gggcgctcgc gatcctggtc gtctcgacgt
     2581 gggtattcgg ggtgaccttc agcgtccagc agtggggcct gctgatcgcg gtgttcttcc
     2641 tgaccctgtc ggccctgtat ggcctcggga tggccatggc cagcctgtac ctcctgtggg
     2701 gccgcgaggc attccacctg accaacgtga tgatcgagcc cgtgtacttc gtgtccgggc
     2761 tgaacttccc ggtcggcaag ctgggcgcgc tgggcatggt ggcgatcgcg acgatcccgt
     2821 tcgccgtcgg cctcgacgcg atgcgccagc tggtgttcac gggccagcca tacatcaccg
     2881 gcacaccctc gcccgaggtc gaggcgctga tcctcgtcgc gatgaccgtc gggttcacgc
     2941 tgctcgcccg gtacctgatc cggcggatcg agcgaatggc ccgcgcacgc ggcacgcttt
     3001 cgctgcgctg gcaatgagcg ccatcaccgg cgagggcctg gccggcacct cgcccacctt
     3061 cgacctcgga cgaacggagg gcggcggcgc gctcgagacg ggccgcaacc tgcggaccgc
     3121 gctgctgctc ggctggcgcg tcgagtcgaa ctggaccgat cccgtcctgt tcttcatcta
     3181 cacggtcgcg cggccgatcg cgtcgctgct gctgctggtc gcgatggtga cgatcatcgg
     3241 tggcgccgcg aacgaggagg ttcgcacctt cgtcgtcctg ggcagtgccc tgtgggcgat
     3301 gctcgtggcg ggcctgtcgg gaccggcctg gtccgtgctc gaggaccgcg agcggtaccg
     3361 gatgctcaa
//
LOCUS       DBNHXO010000391         1855 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1504909, whole genome shotgun sequence.
ACCESSION   DBNHXO010000391 DBNHXO010000000
VERSION     DBNHXO010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1504909"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="AC162A_07975"
     CDS             <1..693
                     /locus_tag="AC162A_07975"
                     /inference="COORDINATES: protein motif:HMM:NF013768.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010817.1"
                     /translation="ASVTRAAGLATPAKPTSAHLRRLDLVSISDRMASLILVLREGSL
                     KQVLISLDEPIEQTELSRVAAMLNHLLEGRTAAEARQAIGALDAADPALDLPRRVGQR
                     VVVALEEFDAAAIEELFSDGLLNVMAAPEFAQSEKLRRVFAALENRAYLAHLIAGVSG
                     SDTVRVYIGHENSAEDMADVSLILAPYGRPGRGVGVVGVLGPTRMAYPQAIGTVRFVS
                     GLMNELIDHLYA"
     gene            715..1281
                     /locus_tag="AC162A_07980"
     CDS             715..1281
                     /locus_tag="AC162A_07980"
                     /inference="COORDINATES: protein motif:HMM:NF013212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR5010818.1"
                     /translation="MARKTRAQERIEEIDVSPAALSAQIELLQAERDEAVTKADEYVL
                     ALQRERAEFTNFRRRTSEERERELGLAGEDLIRKVLAVADDFDRAIESRPAELEGDPW
                     ADGIAVIDRKLRQLLDSEGVTSVEASPGTAFDPREHEAIVSVPGTGRPEGEIVEEVRR
                     GYRLRDRVIRPALVAVATAEDAAAGLAN"
     gene            1338..>1855
                     /locus_tag="AC162A_07985"
     CDS             1338..>1855
                     /locus_tag="AC162A_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR5010819.1"
                     /translation="MGKIIGIDLGTTNSVVAVMEGGEPTVIPSSEGNRTVPSVVGFAK
                     TGERVVGQLAKRQAVTNPTNTVYSIKRFMGRRWDDPEVQRSKDLVPYTVEKDSKSEGI
                     KVVADGKGYTPPEISAMVLQKLKADAEAYLGEKVTEAVITVPAYFDDTQRQATKDAGR
                     IAGLEVKRIINEP"
BASE COUNT          319 a          663 c          630 g          243 t
ORIGIN      
        1 gcctcggtca cccgcgcagc gggcctcgcg acgcccgcga agccgacgtc cgcgcacctg
       61 cgccgcctgg acctcgtctc gatcagcgac cgcatggcga gcctcatcct cgtcctccgc
      121 gagggctcgc tcaagcaggt cctgatctcg ctcgacgagc ccatcgagca gacggagctg
      181 tcccgcgtgg cggcgatgct caaccacctg ctcgagggcc ggacggccgc cgaggccagg
      241 caggccatcg gcgcgcttga cgcggcggac ccggcgctcg acctaccccg gcgggtgggc
      301 cagcgagtcg tcgtcgcgct cgaggagttc gacgccgccg ccatcgagga gctgttctcc
      361 gacggcctgc tgaatgtgat ggcggccccg gagttcgcac agagcgagaa gctgcggcgc
      421 gtgttcgcgg ccctcgagaa ccgcgcctat ctcgcccacc tgatcgccgg cgtgtccgga
      481 tcggacacgg tccgcgtgta catcgggcac gagaactcgg ccgaggacat ggccgacgta
      541 tcgctcatcc tcgctcccta tgggcggcct ggacgcggcg tcggcgtcgt gggcgtgctg
      601 gggcccacgc gcatggccta cccccaggcg atcggcacgg tccgcttcgt ctcgggcctg
      661 atgaacgagc tgatcgacca cctgtacgcc tgacccgaga cgagacggag aacaatggcc
      721 cggaagaccc gcgcccagga gcgcatcgaa gagatcgacg tgtcccccgc cgccctgtcg
      781 gcacagatcg agctgctgca ggccgaacgc gacgaggcgg tcacgaaggc ggacgagtac
      841 gtacttgcgc tgcagcgcga gcgcgcggag ttcacgaact tccggcgacg gaccagcgag
      901 gagcgcgaac gtgagctcgg ccttgccggc gaggacctca tccgcaaggt gcttgccgtc
      961 gccgacgact tcgaccgcgc catcgagagc cggccggcgg agctcgaggg tgacccgtgg
     1021 gccgatggca tcgcggtcat cgaccggaag ctccgccagc tgctcgacag cgagggcgtg
     1081 acgtcggtcg aggcctcgcc cggcacggcc ttcgatcctc gcgagcacga ggcgatcgtc
     1141 agcgtcccgg gcacgggccg gcccgagggc gagatcgtcg aagaggtgcg gcgcggctac
     1201 cggctgcgcg accgtgtcat ccgcccggcg ctcgtggccg tcgcgaccgc cgaggacgca
     1261 gccgccgggc tcgccaactg atccatccaa ccgactgaac caccacccga accacaacga
     1321 ccaggagagc gcctcgcatg ggcaagatca tcggcatcga cctcggaacg acgaacagcg
     1381 tcgtggcggt catggaaggc ggcgagccga ccgtgattcc ctcgtccgag ggcaatcgca
     1441 ccgtcccgtc ggtcgtcggc ttcgccaaga ccggcgagcg cgtcgtcggc cagctggcca
     1501 agcgccaggc ggtcaccaac cccaccaata ccgtctattc gatcaagcgc ttcatgggcc
     1561 gtcgctggga cgaccccgag gtgcagcggt cgaaggacct cgtgccgtac acggtcgaga
     1621 aggactccaa gtccgagggc atcaaggtcg tcgccgacgg caagggctat accccgcccg
     1681 agatctcggc gatggtcctc cagaaactca aggccgacgc cgaggcgtac ctcggggaga
     1741 aggtcaccga ggccgtcatc acggtcccgg cctacttcga cgacacgcag cgccaggcga
     1801 ccaaggacgc cggccggatc gcgggcctcg aggtcaagcg gatcatcaac gagcc
//
LOCUS       DBNHXO010000392         2233 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_315126, whole genome shotgun sequence.
ACCESSION   DBNHXO010000392 DBNHXO010000000
VERSION     DBNHXO010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_315126"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="AC162A_07990"
     CDS             <1..506
                     /locus_tag="AC162A_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5010820.1"
                     /translation="PMLCPVCRHDNFEGEDTCANCGADLAASDVPQPAIEFRDTILGS
                     HLDALGVGEPNLVDPALPVSDAVRRMHEAGTDCLLVCDGDRLVGIFTDRDAVVKVAGK
                     AATAAVRDVMTRDPVVLRPDDRLAVAIHKMAVGGFRHVPVVDAKGRPTAVVSAADVFR
                     HIVAALG"
     gene            503..949
                     /locus_tag="AC162A_07995"
     CDS             503..949
                     /locus_tag="AC162A_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010821.1"
                     /translation="MTDTTDLGRPRIAVVADDLIWATRLGDGVRRAGGEPVAIRSTAA
                     LEATQPTVKGAIVDLTARAYDGIATVALAAAAGIPVIAVGQHDDAETRRAATAAGASR
                     VYAYRALFEHGDRVLGAWIETIAPTAQPPDTTEAPMPVTTTPEGVA"
     gene            946..2115
                     /locus_tag="AC162A_08000"
     CDS             946..2115
                     /locus_tag="AC162A_08000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019075100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR5010822.1"
                     /translation="MSGAIAPERYSDRLARAQQVTQAAGLDALLVGVGAELRYLTGYL
                     AMPLERLTMLVIPAVAGAPFTIIAPRLEVTPARTCSAAASGAASVATWEETEDPMFLV
                     AATLEAALGRPARDLGAVAVSDGLRASFVLGLQRVLPGARFSLASAVLREIRMRKDAD
                     EVELLRAAAHAADRVVGAISAGPLVGRTEADVAHEVRERLVAEGHDLAEFWIIASGPN
                     SASPHHDPGERVIRAGEPIVIDIGGTIEGYGSDITRTIWVTGGDPSLGPDETFRRLYD
                     ALQDGQEEATAAVRPGVPCERVDAVARGIIDAAGYGPNFFHRTGHGIGLEGHEDPYLV
                     AGNAEALAPGMAFSVEPGIYLEGRYGARIEDIVVCGETGPDVLNEAPRELLVVNG"
BASE COUNT          295 a          829 c          821 g          288 t
ORIGIN      
        1 cgccgatgct ctgccccgtc tgccgccacg acaacttcga gggcgaggac acctgcgcca
       61 actgcggcgc cgacctcgcg gcgtccgacg tcccgcagcc ggcgatcgag ttccgcgaca
      121 cgatcctcgg cagccacctc gacgccctgg gcgtgggcga gccgaacctc gtcgaccccg
      181 cgctgcccgt ctcggacgcg gtccgccgga tgcacgaggc cgggaccgac tgcctgctgg
      241 tgtgcgacgg tgaccgcctg gtcgggatct tcaccgaccg cgacgccgtg gtgaaggtgg
      301 ccggcaaggc cgcgaccgcc gcggtgcgcg acgtcatgac gcgcgacccg gtcgtcctcc
      361 ggcccgacga ccgcctcgcg gtcgcgatcc acaagatggc cgtgggcggc ttccgccacg
      421 tgccggtggt ggacgcgaag ggccggccca ccgcggtcgt gtcggcggcg gacgtcttca
      481 ggcacatcgt cgctgcgctc gggtgaccga cacgaccgac ctcggccgcc cgcggatcgc
      541 ggtcgtggcc gacgacctga tctgggcgac ccgcctcggc gacggcgtgc gacgggccgg
      601 cggtgagccg gtggcgattc gctcaacggc cgcactcgag gcgacgcagc cgacggtcaa
      661 gggcgccatc gtcgacctga cggcgcgcgc ctacgacggg atcgcgacgg tcgccctggc
      721 cgcggcggcc ggcatcccgg tcatcgccgt gggtcagcac gacgacgccg agacacgacg
      781 cgcggcgacg gccgccggtg cgtcgcgcgt ctacgcctac cgagccctgt tcgagcacgg
      841 cgaccgcgtg ctcggggcct ggatcgagac catcgcccct accgcccaac cacccgacac
      901 gacggaggct ccgatgccgg tcacgaccac cccggaaggt gtcgcatgag cggcgcgatc
      961 gcccccgagc gctattcgga ccggctggcg cgcgcgcagc aggtgacgca ggcggccggc
     1021 ctggacgcgc tgctggtcgg cgtcggcgcg gagctccggt acctcacggg ctacctggcg
     1081 atgccgctcg agcggctcac gatgctcgtg atcccggccg tcgccggcgc cccgttcacg
     1141 atcatcgccc cgcgcctcga ggtgacgccg gcccggacgt gttccgcggc cgcgtcgggc
     1201 gccgcgtcgg tcgccacctg ggaggagacc gaggatccaa tgttcctcgt cgccgcgacg
     1261 ctcgaggcgg cactcggccg gcctgcgcgt gatctcggag cggtcgccgt ctccgacggc
     1321 ctgcgggcgt ccttcgtgct cggcctgcag cgggtgctgc cgggcgcgcg cttctcgctg
     1381 gcctccgcgg tcctgcggga gatccggatg cgcaaggacg ccgacgaggt ggagctgctc
     1441 cgggccgccg cgcacgccgc ggatcgtgtc gtgggggcga tctccgccgg cccgctggtc
     1501 ggtcggaccg aggccgacgt cgcccacgag gtgcgcgagc gcctcgtcgc cgagggccac
     1561 gacctcgccg agttctggat catcgcctcg ggccccaaca gcgcctcgcc ccaccacgac
     1621 ccgggcgagc gcgtgatccg tgccggggag ccgatcgtga tcgatatcgg cggcacgatc
     1681 gagggctacg ggagcgacat cacccgcacg atctgggtca ccggcggcga cccgtcgctc
     1741 ggtcccgacg agacgttccg ccgcctgtac gacgcgctcc aggacggcca ggaggaggcg
     1801 accgccgccg tccggccggg cgtcccctgc gagcgggtcg acgcggttgc tcgcggaatc
     1861 atcgacgcgg ccgggtacgg accgaacttc ttccaccgga caggccatgg gatcggcctc
     1921 gagggccacg aggacccgta tctcgtggcc ggcaacgccg aggcgctggc gccggggatg
     1981 gcgttcagcg tggagcccgg gatctacctc gagggccggt acggcgcgcg gatcgaggac
     2041 atcgtggtct gcggcgagac cggaccggac gtcctcaacg aggcgccgcg ggagctgctg
     2101 gtcgtcaacg gctgatcccg ggagagacgg gggccgcgag cggggggtat catccgccga
     2161 tcggttggcc attcgaccga gccgagagcc cacgatgaca aaccttgacg cgcgagcccc
     2221 aggctcgcgt cgc
//
LOCUS       DBNHXO010000393         1925 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1701468, whole genome shotgun sequence.
ACCESSION   DBNHXO010000393 DBNHXO010000000
VERSION     DBNHXO010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1701468"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..265
                     /locus_tag="AC162A_08005"
     CDS             134..265
                     /locus_tag="AC162A_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010823.1"
                     /translation="MDVMLIAIMLVYGFFVLAAVTVGADSRDMADDPSGWTSHVGLS"
     gene            complement(288..>1925)
                     /locus_tag="AC162A_08010"
     CDS             complement(288..>1925)
                     /locus_tag="AC162A_08010"
                     /inference="COORDINATES: protein motif:HMM:NF020660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HIR5010824.1"
                     /translation="EQGLRYRIPFGAVEDVIGYLRDHATEAGDRVGMMGDDGEKFGAW
                     PTTYAHCWGEGRWIDRFFDALESNRDWLTTVTPSGWLEREPPIGRVYVPTSSYAEMGE
                     WALPPDENVVFVPLLHDAVKAHRPEARWLRGGFWRNFQVKYREINDLHKQMLRTSAKV
                     AAMPMERAKDAAIDHLHRGQSNDCYWHGLFGGIYISHMRLATYEHLIAAEDAADRALG
                     TLETAASLDLDMDGLPEVLLADAGQVVAIKPAEGGGIGGWDIRAARHALAAALRRRPE
                     AYHETLRAHDAAASNAAGGTGGDGGDDDGAPVSIHDIVMVKEEGLAARLFYDDHERRS
                     GLVRFLPPDPTPEAFATAAETELGDLRDGDWTVDHLAPGQASLSRNGVVDGRPITVSK
                     SIRLLGDRLAPELVIDLELHYGGSEAIDTRLGLELSVHLLGGGGNPSAWYDVGGVRSA
                     HDGSGAATAVESIGYGNDWVGVAVEAVPTPPADAWWSPIETVSNSESGFERVYQGSSL
                     LLSWPVRLAPGEARRFSVRQVVRVARDRAVEEAAASA"
BASE COUNT          267 a          698 c          675 g          285 t
ORIGIN      
        1 cgcaggcgat ggccgttcgg cggcggtggc ggatccaatt cggctacttg acatagtgta
       61 ctagtccaac taccgtgcaa cctggcctag cggactatac caaccgctgt cggaagcctc
      121 gaggacgcgc cccatggacg tcatgttgat cgccatcatg ctggtgtacg gcttcttcgt
      181 gctcgctgcg gtgaccgtcg gggccgactc gcgcgacatg gcggacgacc ccagcggctg
      241 gacctcccac gtcggcctga gctgatcttc gcacgcccga gcgggcatca cgcggacgcg
      301 gccgcctcct ccacggcccg atcccgggcg acgcgaacga cctggcggac cgagaagcgt
      361 cgcgcctcgc ccggcgccag gcgcaccggc cacgacagca gcaggctgct gccctgatac
      421 acgcgctcga aaccggactc ggagttcgac accgtctcga tcgggctcca ccaggcgtcg
      481 gccggtggcg tcgggaccgc ctcgaccgcg acgcccaccc agtcgttgcc atacccgatt
      541 gactcgacgg cggtggccgc cccgctgccg tcgtgcgcgg accggacacc gccgacgtcg
      601 taccaggccg acgggttgcc gccgccaccg agcagatgca ccgacagctc gaggcccagc
      661 cgggtgtcga tggcctccga gcccccgtag tggagctcga ggtcgatgac cagctcgggc
      721 gccaggcggt cgccgagcag gcggatggac ttgctgaccg tgatcggcct cccgtcgacg
      781 acgccgttgc gcgacaggga cgcctggccc ggcgcgaggt ggtcgaccgt ccagtcgccg
      841 tcgcgcaggt cgccgagctc cgtctccgcg gcggttgcga acgcctcggg ggtcgggtcg
      901 ggcgggagga agcgcacgag gcccgatcgc cgttcgtggt cgtcgtagaa cagccgggcg
      961 gcgaggccct cctccttgac catgacgatg tcgtggatcg acaccggggc gccgtcgtca
     1021 tcgccgccat cgccaccggt gccgccggcg gcgttcgagg ccgcggcgtc gtgcgcgcgc
     1081 agggtctcgt gatacgcctc gggccggcgg cggagggccg cggccagcgc atgccgggcc
     1141 gcgcggatgt cccagccgcc gatcccgcca ccctccgctg gcttgatcgc gacgacctgc
     1201 ccggcgtcgg cgagcagcac ctcgggcagc ccgtccatgt cgaggtcgag cgaggcggcc
     1261 gtctcgagcg tgccgagcgc ccggtccgcc gcgtcctccg ccgcgatcag gtgctcgtag
     1321 gtcgccaggc gcatgtgcga gatgtagatc ccgccgaaca gcccgtgcca gtagcagtcg
     1381 ttggactggc cgcggtggag gtggtcgatc gcggcgtcct tggcccgctc catcggcatc
     1441 gcggcgacct tggccgaggt gcgcagcatc tgcttgtgga ggtcgttgat ctcgcggtac
     1501 ttcacctgga agttgcgcca gaagccgccc cgcagccagc gcgcctcggg gcgatgcgcc
     1561 ttgaccgcgt cgtggaggag cggcacgaac accacgttct cgtcgggcgg cagcgcccac
     1621 tcgcccatct ccgcgtacga cgacgtcggg acgtatacgc ggccgatcgg cggctcgcgc
     1681 tctagccacc cggatggggt gacggtcgtc agccagtcgc ggttggactc gagggcatcg
     1741 aagaaccggt cgatccagcg gccttcgccc cagcagtgcg cgtaggtggt cggccaagcg
     1801 ccgaacttct cgccgtcatc gcccatcatc ccgacccggt cgccggcctc ggtcgcgtgg
     1861 tcgcgcaggt agccgatgac gtcctcgacg gccccgaacg ggatgcggta gcgcagcccc
     1921 tgctc
//
LOCUS       DBNHXO010000394         2897 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3487382, whole genome shotgun sequence.
ACCESSION   DBNHXO010000394 DBNHXO010000000
VERSION     DBNHXO010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3487382"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="AC162A_08015"
     CDS             <1..644
                     /locus_tag="AC162A_08015"
                     /inference="COORDINATES: protein motif:HMM:NF020660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HIR5010825.1"
                     /translation="RSGLVRFLPPDTTPEAFATVGETELGDLRDGEFAVDHLAPGQAS
                     LSRNGVVDGKPISVSKSIRLLGSRLAPELVIDLELHYGGSEAIDTRLGLELSVHLLGG
                     GGNPSAWYDVGGVRSAHDGSGAAAAVESIGYGNDWVGIAVAAVPTPPADAWWSPIETV
                     SNSESGFERVYQGSSLLLSWPLRLAPGEARRFSVRQVVRVARDRAVEEAAAPT"
     gene            complement(667..798)
                     /locus_tag="AC162A_08020"
     CDS             complement(667..798)
                     /locus_tag="AC162A_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010826.1"
                     /translation="MDVMLIAIMLVYGFFVLAAVTVGADSRDMADEPNGWTSHVGLS"
     gene            878..1915
                     /locus_tag="AC162A_08025"
     CDS             878..1915
                     /locus_tag="AC162A_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR5010827.1"
                     /translation="MSSSRIGSATAAERPSLGANSGGHELDIERGSDVPISTQIYWQL
                     AYQIESGRLQPGTRLPPVRELGAALRVNPNTVRAVYRRLADAGYVVGRHGAGTRVVDR
                     PPVRRGSDALAGIVAETLRRAAQLGFTPDEVAQATFTAASEHRRPGPLVRVLFAECTS
                     ADATFDAERITEAFPERIEAMGVLLEELPERLDRYHYDLVATTTFHADEAQAYVGGAV
                     PVVAMLVGPGYMSLVHEVAGLPPGTKVGLVCGSQRGVENIAEVLALAGATGVEIVSAV
                     AHTEAEMELVDRDADIVLLSREAMALGLDSRFERPERLREWSYEFDPAGLELLRRAIE
                     RVDASREHVPA"
     gene            2005..>2897
                     /locus_tag="AC162A_08030"
     CDS             2005..>2897
                     /locus_tag="AC162A_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010828.1"
                     /translation="MRALPISRTARQRFGLDALAAPAPAAGPRPSGLAADIVAARKVV
                     AKVNATRRAGEPSAQAGELAAFELLHEIFHLLVIKAAELVPETAMPVTTVVVEETVGE
                     PVLGEVLDAVADEFPDVGDRPAPTRLEELLLLRVANENPAARPLRALVDDTPLPALPR
                     QETIAALEAYQADLTPIGPNGETLVELLRAPARAHPTSLAGQLRYIREHWRGLLGAEL
                     DAMLDRLLITLDVITEEERGLHLRFGGGGDGGWGGGHGEAPDLTGLDAEPERFSSDSA
                     WMPRLVLIAKSTHVWLDQLSRR"
BASE COUNT          387 a         1053 c         1055 g          402 t
ORIGIN      
        1 ggcgttcggg gctcgtgcgg ttcctcccgc ccgacacgac ccccgaggcg ttcgcgaccg
       61 tcggggagac ggagctcggc gacctgcgcg acggcgagtt cgccgtcgac cacctcgcgc
      121 cgggccaggc gtccctgtcg cgcaacggcg tcgtcgacgg gaagccgatc agcgtcagca
      181 agtccatccg cctgctcggc tcccgcctgg cgcccgagct cgtgatcgac ctcgagctgc
      241 actacggggg ctcggaggcg atcgacaccc ggctgggcct cgagctgtcg gtgcacctgc
      301 tcggtggcgg cggcaacccc tcggcgtggt acgacgtcgg cggtgtccgg tccgcgcacg
      361 acggcagcgg tgcggccgcc gccgtcgagt cgatcgggta tggcaacgac tgggttggca
      421 tcgcggtggc ggcggtcccg acgccgccgg ccgacgcctg gtggagcccg atcgagaccg
      481 tctcgaactc ggaatccggg ttcgaacgcg tgtatcaggg cagcagcctg ctgctgtcgt
      541 ggccgttgcg tctggcgccg ggcgaggcgc gccggttttc ggtccgccag gtggttcgcg
      601 tcgctcgtga tcgtgccgtc gaggaggcgg ccgcgcccac gtgatgcccg ctcgggcgtg
      661 cgaagatcag ctcaggccga cgtgcgaggt ccagccgttg ggctcgtccg ccatgtcgcg
      721 cgagtcggcc ccgacggtga ccgcggcgag cacgaagaag ccgtacacca gcatgatggc
      781 gatcaacatg acgtccatgg ggcgcgtcct cgaggcttcc gacagcggtt ggtatagtcc
      841 gctaggccaa gttgcacggt agttggacta gtacactatg tcaagtagcc gaatcggatc
      901 cgccaccgcc gccgaacggc cctcgcttgg ggccaactcg ggcggccacg agctcgacat
      961 cgagcgcggc tccgacgtcc ccatctcgac ccagatctac tggcagctcg cgtaccagat
     1021 cgagtcgggc cggcttcagc cggggactcg gctgccaccg gtgcgggagc tcggcgcggc
     1081 gctgcgcgtg aaccccaaca cggtgcgcgc ggtgtaccgc cgcctcgccg acgcggggta
     1141 cgtcgtcggc aggcacggcg cggggacgcg agtcgtcgac cgtcccccgg tccgacgcgg
     1201 ctcggacgcc ctggccggga tcgtcgcgga gacgctgcgg cgcgcggcgc agctggggtt
     1261 cacgcccgac gaggtcgccc aggccacgtt caccgccgcc agcgagcacc gccggccggg
     1321 ccccctcgtg cgcgtcctgt tcgccgagtg cacgtccgcg gacgcgacgt tcgacgcgga
     1381 acgaatcacc gaggcgttcc ccgagcggat cgaggcgatg ggcgtgctgc tcgaggagct
     1441 gcccgaacgc ctcgaccggt accactacga cctcgttgcg acgacgacgt tccacgccga
     1501 cgaggcacag gcgtacgtcg gtggcgcggt accggtcgtg gccatgctcg tgggtcccgg
     1561 ctacatgtcg ctggtccatg aagtggcggg gctgccgccg ggcaccaagg tcgggctggt
     1621 atgtgggtcg cagcgcggcg tcgagaacat cgcggaggtc ctggccctcg cgggagcgac
     1681 cggtgtcgag atcgtgtcgg cggtcgccca caccgaggcc gagatggagc tcgtcgaccg
     1741 cgacgcggac atcgtgctgc tgtcgcgtga ggcgatggcg ctgggtctcg actcccgctt
     1801 cgagcgcccc gagcggctcc gcgagtggag ctacgagttc gaccctgcag gcctcgagct
     1861 cctgcgccgg gccatcgagc gcgtcgacgc ctcgcgggag cacgtcccgg cctaggattc
     1921 gccgccgctg gcgtacgtcg cggcctggca tgccagcggc gccggaacgg cagcgcgcga
     1981 gacgcgaggt gcgacactct cagcatgcga gccctgccca tctcgcgaac cgcgcggcag
     2041 cggttcgggc ttgacgctct cgccgctccg gcccccgccg ccggcccgcg gccgagcggc
     2101 ctcgccgccg acatcgtcgc ggctcgcaag gtcgtcgcga aggtcaacgc cacgcgccgc
     2161 gcgggggagc cgagcgcgca ggccggcgag ctcgcggcgt tcgagctcct ccacgagatc
     2221 ttccacctgc tcgtgatcaa ggcggcggag ctcgtcccgg agacggcgat gccggtcacg
     2281 acggtcgtcg tcgaggagac cgttggcgag ccggtcctcg gcgaggtgct cgacgccgtc
     2341 gccgacgagt tcccggacgt cggcgaccgc cccgcgccca cgcgcctcga ggagctcctg
     2401 ctgctccgtg tcgcaaacga gaacccggcc gcgcggcccc tgcgcgccct ggtcgacgac
     2461 acgccactgc cggccctgcc gcgccaggag acgatcgccg cgctggaggc ctaccaggcc
     2521 gacctgaccc cgatcggccc caacggcgag accctggtcg agctcctccg ggcgccggca
     2581 agggcgcacc cgacgtcgct cgccgggcag ctgcgctaca tccgcgagca ctggcgcggc
     2641 ctcctgggcg cggagctcga cgcgatgctc gaccgcctgc tgatcacgct cgacgtcatc
     2701 accgaggagg agcgcggcct ccacctgcgg ttcgggggtg gcggcgacgg cggctggggc
     2761 ggcggacacg gcgaggcgcc ggacctcacg ggcctggacg ccgagccgga gcggttctcg
     2821 agcgactccg cctggatgcc gcgcctcgtc ctcatcgcca agagcaccca cgtctggctc
     2881 gaccagctgt cgcggcg
//
LOCUS       DBNHXO010000395         2167 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5076149, whole genome shotgun sequence.
ACCESSION   DBNHXO010000395 DBNHXO010000000
VERSION     DBNHXO010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5076149"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="AC162A_08035"
     CDS             <1..146
                     /locus_tag="AC162A_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HIR5010829.1"
                     /translation="ASFYGLSIGIEVLILAFILRSAWTWPRTYSDAADVATADRLQQQ
                     AHA"
     gene            233..961
                     /locus_tag="AC162A_08040"
     CDS             233..961
                     /locus_tag="AC162A_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR5010830.1"
                     /translation="MPALPPRLSLEQVRRLAKDLLRDLKDGDRAAIRRLAVVSAPSTL
                     AGAQLAIAREYGFPSWARLKRDVELREILDSLDVGRLRALVAEEPDLASTTMEHWVDH
                     PAGASPLGYVAMLRYDTARGIWREVDGAGAIAGILVAAGAPVDGQKGVPETPLITAAS
                     YGDAGVARVLIGAGADLEARASDVAGGVPGGTALLHAAVFGMSDVVDVLVEAGARSEG
                     IEVAAAVGDVTEWLAGAAPDALTW"
     gene            1107..1400
                     /locus_tag="AC162A_08045"
     CDS             1107..1400
                     /locus_tag="AC162A_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010831.1"
                     /translation="MARLDSEAEATVVRPSRIPTAAEARTYLESLPELWAKTSDAGRH
                     TIAEAVFEQIDVLGAHDFTFTLTAHAKAHGWDAAFGAEDQSISIGQSGRGERI"
     gene            complement(1379..1455)
                     /locus_tag="AC162A_08050"
     tRNA            complement(1379..1455)
                     /locus_tag="AC162A_08050"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Pro,seq:cgg)
     gene            complement(1527..>2167)
                     /locus_tag="AC162A_08055"
     CDS             complement(1527..>2167)
                     /locus_tag="AC162A_08055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5010832.1"
                     /translation="AAPVSRLYRRVLVDRDLRWVYAAAVLGGGSRGLGVLNIFVPLYL
                     DQVVGLTPQTIGLMYGILLAASVPGPLVAGWLSDRVGRKPVIIGVYLAGAASIALFVL
                     AGSDIVWLWAGIIALSLFSFVESPQLQALLADVTPRHLRDAAFSTYFALAFGVGSMWG
                     IVYGIIIDVSSQGESGGLAAVFWLMAVASILAALATARIRIPKHSGTYATAA"
BASE COUNT          348 a          759 c          761 g          299 t
ORIGIN      
        1 gggcgtcctt ctacggcctc tccatcggaa tcgaggtgct gatcctggcc ttcatcctgc
       61 gctccgcctg gacctggccc cgaacgtact ccgacgctgc agatgtcgcg acggccgacc
      121 gactgcaaca gcaagcccac gcctgagcca tcggtgctcg acgcaggcag gagagtgcct
      181 gcgtcgagcc tgacgctcac cgcggcaacc gagaacaaga ggactcgtcc ccatgcccgc
      241 cctcccgccg cgtctgagcc tcgagcaagt ccggcgcctc gcgaaggacc tgctccgcga
      301 cctgaaggac ggcgatcgcg ccgcaattcg acgcctcgcg gtcgtgtctg caccatcgac
      361 gctggctggc gcgcaactcg ccattgcccg cgaatacgga ttccccagct gggcgcgcct
      421 caagcgcgac gtcgagctgc gcgagatcct cgactctctc gatgtgggcc gcctgcgcgc
      481 gctcgtcgct gaggagcccg acctcgcgtc gaccacgatg gaacattggg tcgatcaccc
      541 agcgggcgcc tcgccgctcg gctacgtcgc gatgctccgg tacgacaccg cccgcggcat
      601 ctggcgtgaa gtcgacggcg caggcgctat cgccggcatc ctcgtcgcgg ccggtgcccc
      661 ggtcgacggc cagaagggtg ttcccgagac gccgctcatc acagccgcga gctacggcga
      721 tgccggtgtc gcccgggtgc tcatcggcgc aggtgccgac ctggaggcac gggccagcga
      781 cgttgcaggc ggggtccccg gcggcacggc gctactccac gcggctgtgt tcgggatgag
      841 cgacgtggtc gatgtgctcg tcgaggcagg ggctcggagt gaggggatcg aggttgcggc
      901 cgccgtcggc gatgtcaccg aatggctcgc tggggcagcg cccgacgcgc tgacgtggtg
      961 agcacggcgc cagcacggtc gcggcggcca cccggccgga gccgcgccgg ggcgacgtcc
     1021 tggcacaagc ccggatcaag cgcgagcgcg agctggcggc gctgcggttc gcgaaggatc
     1081 gcgacctggg tcgactcgag gcgacgatgg cgcggctcga ctccgaggcc gaggctaccg
     1141 tcgtccgccc gtcgcgcatc ccgaccgccg ccgaggcgcg gacgtacctg gagtcgctgc
     1201 cggagctgtg ggcgaagacg tcggatgccg ggcgtcacac gatcgccgag gccgtgttcg
     1261 agcagatcga cgtcctgggg gcgcacgact tcacgttcac gctgacggcc cacgcgaagg
     1321 cccacggatg ggatgctgcc ttcggggctg aggaccagtc aatctcgatt ggtcagtctg
     1381 gtcggggcga gaggatttga acctccgacc tcatcgtccc gaacgatgcg cgctaccaag
     1441 ctgcgccacg ccccgaccga aaggccccgc tgaggggccg gggcatgata gcgcatcccc
     1501 ttcatgcggc cggtcacggc gacctttcag gccgccgtcg catacgtccc gctgtgcttc
     1561 gggatcctga tccgcgccgt cgccagtgcc gcgaggatcg atgccaccgc catcagccag
     1621 aacaccgccg cgaggccccc gctctcgccc tgtgagctga cgtcgatgat gatcccgtac
     1681 acgattcccc acatcgagcc gaccccgaac gccagcgcga agtaggtact gaacgcggcg
     1741 tcgcggaggt ggcgcggcgt gacgtcggcg agcagcgcct gcagctgcgg gctctcgacg
     1801 aacgagaaca ggctgagcgc gatgatcccg gcccatagcc agacgatgtc gctgccagcg
     1861 aggacgaaca gggcgatcga tgccgcgccg gcgaggtaga cgccgatgat cacgggcttg
     1921 cggccaaccc ggtccgacag ccagccggcg acgagggggc cgggcacgct cgcggcgagc
     1981 aggatcccgt acatcaggcc gatggtctgg ggcgtcagcc cgacgacctg gtcgaggtag
     2041 aggggcacga agatgttcag gacgccgagg ccgcgcgaac cgccgcccag gacggccgcg
     2101 gcgtagaccc agcggaggtc acggtcgacg aggacgcggc gatagaggcg actcaccggt
     2161 gccgcct
//
LOCUS       DBNHXO010000396         2976 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3289708, whole genome shotgun sequence.
ACCESSION   DBNHXO010000396 DBNHXO010000000
VERSION     DBNHXO010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3289708"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1092
                     /gene="thyX"
                     /locus_tag="AC162A_08060"
     CDS             235..1092
                     /gene="thyX"
                     /locus_tag="AC162A_08060"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HIR5010833.1"
                     /translation="MAEAPAPQRAKTAIPRNPGRHDLGLQASLLFPDKDIVERFYVPL
                     IYTACRTCYSELEPQEIFRRATAGEIAPASMQRLIGNVIESGHGSTIEHIVFTFAISG
                     VSRTLSHQLVRHRAGVAFDQQSQRYVKYKGAATMLPATIAEGDEALRERYEEQVDGAL
                     GLYGELVEAGVPGEDARFVFPNATRTNLVMTTNLRALIHMSGLRLCTMAQWEIRRLFQ
                     LIRHEIFDVSPFLGSFLAPKCVALGYCDEFNNRDEHCPIRPHKDNVLAAWAQKRDEAK
                     KAGRELPVT"
     gene            1110..1304
                     /locus_tag="AC162A_08065"
     CDS             1110..1304
                     /locus_tag="AC162A_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010834.1"
                     /translation="MNRPSKTVTSIGVVAFVIAAAMTGIAGLGGAMSGYDRLGLLFIG
                     IFLGVFLLGFLLDRAAKRAL"
     gene            complement(1327..2226)
                     /locus_tag="AC162A_08070"
     CDS             complement(1327..2226)
                     /locus_tag="AC162A_08070"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012686075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR5010835.1"
                     /translation="MSDFFSPGIDRLRPIPPADQPARLEALVEAVQGRTAIVPRVGIV
                     LGSGLGGLAYEVENPIAIPFDQLPGWPIATAPGHEGRLLLGRLGGVQVVILQGRFHLY
                     EGNDPGLVVQPVLLFRKLGAKTVILTNAAGGVNPDYAPGTLMVIADHLNLTGRTPLLG
                     RNADELGPRFPDLTDVWTPALRERLHAAGAAVGVELAEGIYAGLLGPSYETPAEVRML
                     RTMGADAVGMSTVLEAVAARWVGLEVCGVSLVTNAGAGYSGQPLTHAEVMEAGELAGP
                     RLAKVLQRFLADLPAPGSAGSGA"
     gene            complement(2637..>2976)
                     /locus_tag="AC162A_08075"
     CDS             complement(2637..>2976)
                     /locus_tag="AC162A_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010836.1"
                     /translation="RTEVPLPIQGDRRAWDAVVIAHDGWTAIEAISRLGAVDATLRTT
                     NQKQRDDLRIARTVLVIADTPRNRAALRLAHATVRAEYPLDTRSVLAALGEGKAPTAS
                     GIVLLRLRNE"
BASE COUNT          446 a         1054 c         1033 g          443 t
ORIGIN      
        1 gcgggctgtg gtacgcggcc ctccaggcga cgctcggctt cgaggccggc tacacggtca
       61 acttcatcac gatcatcacg ctgtacacga tcgcgacctg cctgtactgg atctggttcc
      121 gggacactga ccggcgcacg atggcggtac gggtggccgc ctagctcccg cacggcggcc
      181 gtttcactgc attcggctga tccgcggcgg cgacgcgccg gtagacttcg acccatggca
      241 gaagccccgg caccgcagcg cgccaagacc gcgatcccgc gcaatcccgg ccgccacgac
      301 ctcgggctgc aggcgtcgct cctgttcccg gacaaggaca tcgtggagcg cttctacgtg
      361 ccgctgatct acaccgcctg ccggacgtgc tacagcgagc tcgagcccca ggagatcttc
      421 cgccgggcca ccgcgggcga gatcgcgccg gcctcgatgc agcgcctgat cggcaacgtc
      481 atcgagtccg gacacggctc caccatcgag cacatcgtgt tcacgttcgc catctcgggc
      541 gtctcgcgca cgctgtccca ccagctcgtg cgccatcgag cgggcgtggc gttcgaccag
      601 cagagccagc gctacgtcaa gtacaagggc gccgcgacga tgctgccggc gacgatcgcc
      661 gagggcgacg aagccttgcg cgagcggtac gaggagcagg tcgacggcgc gctggggctg
      721 tacggggagc tcgttgaggc cggcgtgccc ggtgaggacg cacggttcgt gttccccaac
      781 gcgacgcgca cgaacctcgt catgacgacc aacctgcgtg cgctcatcca catgtcgggc
      841 ctgcgtctgt gcacgatggc ccagtgggag atccgccggc tgttccagct gatccgccat
      901 gagatcttcg acgtgtcgcc gttcctgggc tcgttcctcg ccccgaagtg cgtagcgctc
      961 ggctactgcg acgagttcaa caatcgcgac gagcactgcc ccatccggcc gcacaaggac
     1021 aatgtcctgg ccgcctgggc gcagaagcgc gacgaagcca agaaggccgg ccgggagctg
     1081 ccggtcacct gacgagggag cccaaccgca tgaaccgccc cagcaagacc gtgacctcga
     1141 tcggcgtggt cgcgttcgtc atcgccgccg ccatgaccgg catcgccggc ctcgggggcg
     1201 ccatgtccgg ctacgaccgc ctcggcctgc tgttcatcgg catcttcctc ggcgtgttcc
     1261 tgctcggctt cctcctcgac cgcgcggcga aacgagcgct ctagcggccg gtcgccgcga
     1321 tcctcgtcag gcgccggagc cggctgagcc gggcgccggg aggtcggcaa ggaagcgctg
     1381 cagcaccttc gccaggcgcg ggccggccag ctcgcccgcc tccatcacct cggcgtgggt
     1441 cagcggctgg ccgctgtagc cggctcccgc gttggtgacg agcgagacgc cgcacacctc
     1501 cagccccacc cagcgggcgg cgaccgcctc gagcacggtc gacatgccga cagcgtcggc
     1561 gcccatcgtt cgcagcatcc gcacctcggc cggggtctcg tacgaggggc cgagcagccc
     1621 ggcgtagatg ccctccgcga gctccacgcc caccgcggcc ccggccgcgt gcagccgctc
     1681 gcgcagggcc ggcgtccaga cgtcggtgag gtccgggaac cgcgggccga gctcgtcggc
     1741 gttgcgcccg aggagcggcg tccgcccggt gaggttgagg tggtcggcga tgaccatcag
     1801 cgtccccggc gcgtagtcgg ggttcacgcc acccgccgcg ttggtcagga tcacggtctt
     1861 cgcgcccagc ttgcggaaca acaggacggg ctggacgacg aggcccggat cgttgccctc
     1921 gtacaggtgg aaccggccct ggaggatgac gacctgcacc ccgccgagac gcccgagcag
     1981 cagccgtccc tcgtgaccgg gcgcggtcgc gatgggccag cccgggagct ggtcgaaggg
     2041 aatcgcgatc gggttctcga cctcgtaggc gagcccgccc aggcccgagc cgagcacgat
     2101 cccgacccgc ggcacgatcg cggtccggcc ctgaacggcc tcgacgaggg cctcgagacg
     2161 ggccggctgg tcggccggcg ggatgggtcg caaccggtcg atgcccgggg agaagaagtc
     2221 gctcatcggc cgaggatagt cgcgcacggg gcgggagtgg gggcccgggc gccttaaact
     2281 tcaacgcccg gatggagtac tgacaccggg atgccagtcg cttccattcg ggcgtccagg
     2341 accgcgaccc ttgcgggccg ccaccgctgg tatgaagtac ccgaattgac cggagagcgc
     2401 cgacccttga tgtgatcgcg gatcgacgtc ggcgtggcgt tgaggtgatc gcagccggcc
     2461 gggcggagca ggtttgaggc gatcgcctcc gctccggacc ttccatcggc cccttcgggc
     2521 aagacctacg ttacggtggc cggggaagcg atccaacggg gttttccacg aacctgacgt
     2581 tcgggcggcc gtggacgtcc accacttttc ccccgttgtg gacgagttgt ggacgatcac
     2641 tcgttcctga gacggagaag gactatcccc gacgccgtcg gggctttgcc ctcgccgagc
     2701 gcggcgagca cggagcgagt gtcaagcggg tactccgcgc gcacggtcgc gtgggccagg
     2761 cggagcgcgg cgcggttccg cggcgtgtcg gcaatgacga ggactgtccg cgcgatccgc
     2821 aggtcgtcgc gttgcttctg gttcgtcgtg cgcagcgtcg cgtcgacggc gcccagccgg
     2881 ctgatcgcct cgatcgccgt ccagccgtca tgagcgatca cgaccgcgtc ccaagcgcgg
     2941 cggtcgccct ggatcgggag gggcacttcc gtccgc
//
LOCUS       DBNHXO010000397         1975 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1109341, whole genome shotgun sequence.
ACCESSION   DBNHXO010000397 DBNHXO010000000
VERSION     DBNHXO010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1109341"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="AC162A_08080"
     CDS             <1..1251
                     /locus_tag="AC162A_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5010837.1"
                     /translation="AEHKERLARAMTREMGKVIAEARGDVQEGIDIAFLMAGEGRRMF
                     GDTVPSELPDKWAMSIRQPIGVAGIVTPWNFPIAIPCWKTMPALVAGNTVVFKPSSET
                     PHCATLLVELMDEAGFPAGTVNLVTGSGGVVGDAIIDSRDVPVISFTGSSDTGRTIAE
                     RAGRLLKRVSLELGGKNAVVVLRDADLDLAVDGILWSAFGTTGQRCTACSRVIVDRAV
                     ADDLVGRLETRAKALKLGDGLDTATDVGPLVNAPAVAKVERYVGIGRREGELVTGGER
                     AADGDLAHGHFFSPTIFAGVAPMATIAQDEIFGPVLSVVPVDGYDEAMTAANQVRYGL
                     SASIYTRDVNAAFRAMREFETGIVYVNAGTTGAETHLPFGGWKNTGNGHREAGHAALD
                     TFTEWKAVYVDYSGRLQRAQIDNQ"
     gene            1332..>1975
                     /locus_tag="AC162A_08085"
     CDS             1332..>1975
                     /locus_tag="AC162A_08085"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015237287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5010838.1"
                     /translation="MWAVELRKTFDVPVREAGIRAATKSLVRREMREVAAVDGISFEI
                     AAGEVVGFLGPNGAGKTTTLKMLSGLLYPTSGEARVLGHTPSKRERDFLRRITMVMGN
                     RNQLQWDLPALDSFDLLRAIYRLPRPEFVAMRDELIELLDLGDLVSKPVRNLSLGERM
                     KVEICGALLHRPSVLFLDEPTIGLDVTAQKRIRSFVAEYNQRTGATVLLTSHYMA"
BASE COUNT          301 a          679 c          713 g          282 t
ORIGIN      
        1 gccgagcaca aggagcggct cgcccgcgcg atgacccgcg agatgggcaa ggtgatcgcg
       61 gaggcgcgcg gcgacgtcca ggaggggatc gatatcgcct tcctgatggc cggcgagggc
      121 cgccggatgt tcggcgacac cgtgccctcg gagctgcccg acaagtgggc gatgagcatc
      181 cggcagccga tcggcgtggc cgggatcgtg acgccctgga acttcccgat cgcgatcccg
      241 tgctggaaga ccatgccggc gctcgtcgcc ggcaacaccg tcgtgttcaa gccgtcgtcc
      301 gagacgccgc actgcgcgac cctcctcgtc gagctcatgg acgaggcggg cttcccggcc
      361 gggaccgtga acctcgtcac gggttcgggg ggcgtggtcg gagacgcgat catcgacagc
      421 cgcgacgtgc cggtgatctc gttcacgggc tcgtcggaca ccgggcgaac gatcgcggaa
      481 cgggccgggc gcctgctcaa gcgggtcagc ctcgagctcg gcgggaagaa cgcggtggtc
      541 gtgctgcggg acgcggacct cgacctcgcg gtggacggaa tcctgtggtc cgcgttcggc
      601 accaccggcc agcggtgcac ggcctgctcg cgggtcatcg ttgatcgagc ggtcgccgac
      661 gacctcgtcg ggcgcctcga gacgcgcgcg aaggctctca agctcggcga tggactggat
      721 acagcgaccg acgtcgggcc gctggtgaac gcgccggcgg tggccaaggt cgagcgctac
      781 gtcgggatcg ggcggcggga gggcgagctc gtcactggcg gggaacgggc cgccgacggc
      841 gatctcgccc acggtcactt cttcagcccc acgatcttcg ccggcgtcgc cccgatggcc
      901 acgatcgccc aggacgagat cttcgggccc gtgctgtcgg tcgtcccggt cgacggctac
      961 gacgaggcga tgaccgcggc caaccaggtc cggtacggcc tctcggcgtc gatctacacg
     1021 cgggatgtca acgccgcctt ccgtgccatg cgcgagttcg aaaccggcat cgtctacgtc
     1081 aacgccggga ccacgggcgc cgagacgcac ctcccgttcg gcggctggaa gaacacgggc
     1141 aacggccacc gcgaggcggg ccatgccgcc ctcgacacgt tcaccgagtg gaaggccgtg
     1201 tacgtggact acagcgggcg cctccagcgc gcccagatcg acaaccagtg accgcggtcg
     1261 ggccggggaa gcaggccggc ggcgcccccg acgagctccc gctgggtgcc gccggcgcgg
     1321 cggacgtcgc ggtgtgggcc gtcgagctgc gcaagacgtt cgacgtcccg gtccgcgagg
     1381 cggggattcg cgcggccacc aagagcctcg tgcggcgcga gatgcgcgag gtcgccgcgg
     1441 tcgacgggat cagcttcgag atcgccgcgg gcgaggtcgt cggcttcctc gggcccaacg
     1501 gggctggcaa gacgaccacg ctcaagatgc tgtcagggct gctgtatccg acgtctggcg
     1561 aggcaagggt gctcggccac acgccgtcca agcgcgagcg ggacttcctg cgccggatca
     1621 cgatggtgat gggcaaccgg aaccagctgc agtgggacct gccggcgctc gactcgttcg
     1681 acctcctgcg cgcgatctat cgcctgccgc gtccggagtt cgtcgccatg cgggacgagc
     1741 tcatcgagct gctcgacctc ggcgacctcg tcagcaagcc ggtccgcaac ctctccctcg
     1801 gggagcggat gaaggtcgag atctgtggcg cgctgctgca tcggccctcc gtcctgttcc
     1861 tcgacgagcc gacgatcggg ctcgacgtca ccgcccagaa gcggatccgt tcgttcgtcg
     1921 ccgagtacaa ccagcggacc ggcgccacgg tgctgctcac gagccactac atggc
//
LOCUS       DBNHXO010000398         2052 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_115382, whole genome shotgun sequence.
ACCESSION   DBNHXO010000398 DBNHXO010000000
VERSION     DBNHXO010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_115382"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1238)
                     /locus_tag="AC162A_08090"
     CDS             complement(9..1238)
                     /locus_tag="AC162A_08090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5010839.1"
                     /translation="MTPTAGYNPCLPVRRAASRTRARTRVVQVLATGTNGGAQEHVYN
                     LVSRMDHERYEVSIVSLSPGSAVRKLERLGIPVTVIDEPDDAIATGILAAHLTDVRAD
                     IVHNHMYRAEIVGTKAVIALGEAGHRRPWVISTVHSSRVRSSEDQEELRRLTGSIDHL
                     IVVSNAIDRKVVAEGRTDAPRSLIYNGIDLVRYDHQEPCCTLRDEYGMEPTSPIVGVV
                     GRLELEKGHPTLLEAWPLVLEACPSAYLLIVGEGSRLDALHDIAREQGIDGHVVFTGR
                     RDDIPAVTAAFDVAVLPSYREAQGLTILEAMALSRPVVASNVGGIPEMIEDGVTGLLV
                     RPQDPEALAAAIIRVLSDHQLADVLGRAGHDLVHDRFCVELMVSAVQDLYDVGASAVR
                     PRELTPAAAGRPSAAAS"
     gene            complement(1235..>2052)
                     /locus_tag="AC162A_08095"
     CDS             complement(1235..>2052)
                     /locus_tag="AC162A_08095"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019041.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5010840.1"
                     /translation="AVFFGASYHADYATLLAAPDDLPAVADAVADYLAHGGDPSHPHP
                     WDAVDLRRLRCGDAAADALAGAFGRREANCGWTMNVEQEDVCPVVTLPSGASMDDFLA
                     TLGKKERHEIRRKVRRAEAAGELALTDSRDPLADLPAFIDLHQKRWGADGLFPPNPGG
                     DQSRTFVRRMFELMGADGPLRLAFLTVGDRRIAAGLTFESPRGILYYNAGVDPDAREL
                     SPGVLMVERYIRRAVERGIPRFDLLRGDEGYKYEWGAVDEPIQRVLVRRTEGR"
BASE COUNT          277 a          720 c          766 g          289 t
ORIGIN      
        1 gacgcggatc agctcgcggc ggccgacgga cgaccggcgg cggccggcgt cagctcgcgc
       61 ggacgcacgg cggacgcccc cacgtcgtag aggtcctgca cggccgagac catcagctcg
      121 acgcagaacc ggtcgtggac gagatcgtgg ccggcccgcc ccagaacgtc cgccagctgg
      181 tgatctgaca gcacccggat gatggcggcg gccagcgcct cggggtcctg gggccggacg
      241 agcaggcccg tgacgccgtc ctcgatcatc tccgggatcc cgcccacgtt cgacgcgacg
      301 acgggtcgcg acagcgccat cgcctcgagg atcgtcaggc cctgggcctc gcggtacgag
      361 ggcaggaccg cgacgtcgaa cgccgcggtg accgcgggaa tgtcgtcgcg ccggccggtg
      421 aacacgacgt gcccgtcgat gccctgctcg cgcgcgatgt cgtgcagcgc atcgagccgg
      481 ctcccctcgc cgacgatcag caggtacgcg gacgggcagg cctcgaggac cagcggccac
      541 gcctcgagca gcgtcgggtg acccttctcg agctccagcc ggcccaccac gccgacgatc
      601 ggcgaggtcg gttccatgcc gtactcgtcg cgcagcgtgc agcacggctc ctggtggtcg
      661 tagcggacga ggtcgatgcc gttgtagatg agcgagcgcg gcgcgtccgt ccggccctcg
      721 gcgacgacct tgcgatcgat ggcgttcgag accacgatca ggtggtcgat cgatcccgtc
      781 agccggcgca gctcctcctg gtcttcgctc gatcggaccc gcgacgagtg gacggtcgag
      841 atcacccacg gtcgccggtg gccggcctcg ccgagggcga tgacggcctt cgtgcccacg
      901 atctcggcgc ggtacatgtg gttgtggacg atgtccgccc ggacgtccgt gaggtgtgcg
      961 gcgaggatgc cggtggcgat cgcgtcgtcg ggctcgtcga tcacggtgac cgggatgccc
     1021 agccgctcga gcttgcgcac cgccgatccc ggggacagcg agacgatcga cacctcgtac
     1081 cgctcgtggt ccatgcgcga gaccaggttg tagacgtgct cctgggcgcc gccgttggtg
     1141 ccggtcgcga ggacctggac gacgcgcgtc cgcgccctcg tccggctggc ggctcgccgg
     1201 acggggaggc acgggttgta gcccgcggtg ggtgtcatcg cccttccgtt cgtcggacga
     1261 gcacgcgctg gattggctcg tcgacggcgc cccactcgta cttgtagccc tcgtcgccgc
     1321 gcaggaggtc gaatcggggg atcccgcgct cgacggcgcg ccggatgtag cgctcgacca
     1381 tcagcacgcc gggcgagagc tcgcgggcat ccgggtcgac gccggcgttg tagtacagga
     1441 tcccgcgcgg cgactcgaag gtgagccccg cggcgatccg ccggtcgccg accgtgagga
     1501 acgcgagccg cagcggcccg tctgcgccca tcagctcgaa catgcggcgg acgaacgtcc
     1561 ggctctggtc gccgccggga ttgggcggga acaggccgtc ggcgccccag cgcttctggt
     1621 ggaggtcgat gaatgccggc aggtcggcca gcgggtcccg cgagtccgtg agcgccagct
     1681 cgcccgccgc ctcggcgcgc cggaccttgc gccggatctc gtggcgctcc ttcttgccca
     1741 gggtcgcgag gaagtcgtcc atcgaggcgc cggacgggag cgtcacgacg gggcagacgt
     1801 cctcctgctc gacgttcatc gtccagccgc agttggcctc gcggcgcccg aaggcgccgg
     1861 ccagggcgtc ggccgcggcg tcgccgcagc ggaggcggcg gagatccacg gcgtcccacg
     1921 ggtgcgggtg gctcgggtcg ccgccgtggg ccaggtagtc ggcgaccgcg tcggcgaccg
     1981 ccggcaggtc gtctggcgcc gcgagcagcg tcgcgtagtc ggcatggtag gaggcgccga
     2041 agaacacggc ct
//
LOCUS       DBNHXO010000399         3820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4282800, whole genome shotgun sequence.
ACCESSION   DBNHXO010000399 DBNHXO010000000
VERSION     DBNHXO010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4282800"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="AC162A_08100"
     CDS             complement(<1..772)
                     /locus_tag="AC162A_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013020262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR5010841.1"
                     /translation="MRLISVRAGSGSTLGVQAGERWLPAGELVPGGPRTIAELIGGGL
                     EAVAALRPAVDVDRILRDGRSVAESELLAPVPRPGKVVAIGRNYRDHAAEEGVDAPPA
                     PLIFAKWPSSVVGPDADVRWDPALTQQVDYEAELAVVIGRTARRVAEADALDHVLGYT
                     CLNDVSARDLQFGDGQWTRGKSLDTFCPMGPALVTADEIPNPQALAIECLLNGRVMQR
                     ANTADMYFGVAAIISHCSWAFTLEPGDVIATGTPGGVGI"
     gene            894..1922
                     /locus_tag="AC162A_08105"
     CDS             894..1922
                     /locus_tag="AC162A_08105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5010842.1"
                     /translation="MPYEPFERIPLGRSGLRVTRLGLGGASIGGLYRPVADADAITLV
                     RHAWDLGIRAFDVAPLYGYGSAERRMGAALAGQPRDEYVLSTKVGRLVLPTERLEPGM
                     DVDHQALDGRDDAFYTGTAGRHVVFDFSAEGVRRSIAESVERLGLERIDVAWIHDPDD
                     HWQAAITGAYPELHRLREAGVVGVIGVGMNQSPMLARFVRETDIDAILLAGRYTLLDQ
                     DALGELLPACVDRGVAVVVGGVMNSGVLADPRPGAAFNYSPAPPEILERARGLAEACE
                     RHGVPVRAAAMQFPLAHPAVVGLIAGVRTPAHLDEYPELLRLPIPASLWSELGTSGLI
                     ATGAPVPA"
     gene            1919..2773
                     /locus_tag="AC162A_08110"
     CDS             1919..2773
                     /locus_tag="AC162A_08110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5010843.1"
                     /translation="MTFPVVDAHHHLWDPARASYPWMTADLDPIRRRFGVEDLVPLLD
                     AAGVAGTVLVQTRSSSAESREFLATAAGTPRIAGVVAWTDLTSQGVDDDIAALRDAPG
                     GDRLVGIRHQVHDEDDAAWLLRADVRRGLLAVERAGLVYDLLVRAREMPVALEVVRAM
                     PDLRFVVDHLAKPPVVDGAMSPWSELLEPFGELPNVTCKLSGLVTEADWSSWTIADLE
                     PYVDRALELFGPDRMLFGSDWPVSLVAAPYQTVFDTAQALLAGLSGDERAAVMGGTAV
                     EVYDLRID"
     gene            2889..3368
                     /locus_tag="AC162A_08115"
     CDS             2889..3368
                     /locus_tag="AC162A_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR5010844.1"
                     /translation="MVRASGPVSLRDTWFGARLPEEAVTRLEPHVRRATYDAGSEILR
                     EGDPTSALGIVVSGRVALRLRVPERGPTTVLTAEPGNIVGWSAVVPPHRATSTAVALV
                     PTELLLLDGEALRSQLDGDPVLAAAVYRSLLEAVAHRLNGTRMQLLDLFAQQGDEPW"
     gene            3365..>3820
                     /locus_tag="AC162A_08120"
     CDS             3365..>3820
                     /locus_tag="AC162A_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5010845.1"
                     /translation="MTATITPAFLVRADLGRLFDLLHADGHRIIGPTVRQGAIVYAEI
                     ATPADLPTGWTATQAPGTYRLQPTGTDRAFDFAVGPTSWKHETFPPRIPLTVGRRVDG
                     QATFEDAAPEAPRVAFLGVRGCEIAALLTQDAVLALGPAVDRDYVARRAA"
BASE COUNT          487 a         1409 c         1377 g          547 t
ORIGIN      
        1 agatcccgac cccgcccggc gtcccggttg cgatgacgtc gccgggctcg agggtgaacg
       61 cccacgagca gtggctgatg atcgccgcga ccccgaagta catgtccgcc gtgttcgcgc
      121 gctgcatcac gcgcccgttg aggaggcact cgatcgcgag ggcctgcggg ttcgggatct
      181 cgtccgccgt caccaacgcc ggacccatcg ggcagaaggt gtcgagcgac ttgccgcgcg
      241 tccattggcc atcaccgaac tggaggtcgc gcgccgagac gtcgttgagg caggtgtagc
      301 cgaggacgtg atcgagcgcg tcggcctcgg cgacgcgccg cgccgtgcgc ccgatgacga
      361 cggcgagctc ggcctcgtaa tcgacctgct gcgtcagagc cgggtcccag cggacgtccg
      421 cgtccggacc gaccacggag ctcggccact tggcgaagat cagcggggcg ggcggggcgt
      481 cgacgccctc ctcggcggcg tggtcgcggt agttgcggcc gatcgcgacg accttgccgg
      541 gacgcggaac cggggccagg agctcggatt cggcgaccga tcggccgtcc cggaggatcc
      601 tgtccacgtc caccgcgggc cgcagggctg caaccgcctc gagaccgccc ccgatcagct
      661 ccgctatggt tcgcggaccg cccgggacga gttcgccggc aggcagccag cgttcgccgg
      721 cctggacgcc gagggtcgac ccggaccccg cccggaccga gatcaaccgc aaccgtcgct
      781 cctgttcagt atactgtccc gtggccgact gattgaacga tacgccagcc ctccgaacgc
      841 cgtcaaccgg ctgacgggga cggatcggcc gccgcgccgc cgcgaggtgt ccagtgccgt
      901 acgagccctt cgaacggatc ccgcttggtc gatccgggtt gcgggtgacg cggctgggcc
      961 tggggggcgc ctccatcggc ggcctgtacc ggccggtcgc ggacgcggac gcgatcacgc
     1021 tcgtccggca cgcctgggac ctcggcatcc gcgccttcga cgtggcgccg ctgtacggct
     1081 acggctcggc ggaacggcgg atgggggcgg ccctggcggg ccagccgcgc gacgagtatg
     1141 tcctctcgac caaggtcggc cggctcgtcc tcccgacgga gcggctggaa cccggcatgg
     1201 acgtcgatca ccaggcgctc gacgggcggg acgacgcgtt ctacaccggg acggccggtc
     1261 gtcacgtcgt cttcgacttc agcgcggagg gtgtgcgccg gtcgatcgcc gagagcgtcg
     1321 agcggctcgg cctcgagcgg atcgacgtcg cctggatcca cgacccggac gatcactggc
     1381 aggcagccat caccggtgcc tacccggagc tgcacaggct ccgggaggct ggggtcgtcg
     1441 gtgtcatcgg ggtgggcatg aaccagtcgc cgatgctcgc gcggttcgtg cgggagaccg
     1501 acatcgacgc gatcctgctc gccgggcgct acacgctgct cgaccaggac gcgctgggcg
     1561 agctgctccc cgcctgcgtc gaccgcggcg tcgccgtcgt cgtcggtggc gtcatgaaca
     1621 gcggcgtgct ggccgacccc cggccgggcg cggcgttcaa ctactcgccg gcgccgccgg
     1681 agatcctgga gcgcgcccgc ggcctggcgg aggcgtgcga gcgccacggc gtgccggtcc
     1741 gggcggccgc gatgcagttc ccgctcgccc acccggccgt cgtcgggctg atcgccggtg
     1801 tccggacccc ggcccatctg gacgaatacc ccgagctcct ccggctcccg atccccgcgt
     1861 cgttgtggtc ggagctcggg acgtctggcc ttatcgcgac gggcgcgccg gtcccggcat
     1921 gaccttcccc gtcgtcgacg cccatcacca tctgtgggac ccggcccgcg cgagctaccc
     1981 gtggatgacc gcggacctcg atcccatccg gcgccggttc ggcgtcgagg acctcgtccc
     2041 cttgttggac gccgcgggcg tcgcgggcac cgtcctcgtc cagacccgct cgtcgtccgc
     2101 cgagagccgc gagttcctcg ccacggcggc aggtacgccg cggatcgccg gggtcgtcgc
     2161 ctggaccgac ctgacgagcc agggcgtcga cgacgacatc gccgcgctgc gtgatgcgcc
     2221 gggcggcgac cggctggtcg ggatccgtca ccaggtgcac gacgaggacg acgcggcatg
     2281 gctcctgcgc gccgacgtcc ggcgcggcct cctggccgtg gagcgggcgg gcctcgtcta
     2341 cgacctgctc gtccgggccc gcgagatgcc cgtggcgctc gaggtcgtgc gggcgatgcc
     2401 ggacctgcgg ttcgtcgtcg accacctcgc caagccgccg gtcgtcgacg gcgccatgtc
     2461 gccgtggtcc gagctcctcg agccgttcgg cgagctgccc aacgtgacgt gcaagctctc
     2521 gggcctggtc accgaggcgg actggtcgag ctggacgatc gctgacctgg agccgtacgt
     2581 cgaccgggcg ctggagctgt tcggaccgga ccgaatgctg ttcggctcgg actggccggt
     2641 ctcgctcgtc gccgcgccgt accagaccgt gttcgacacg gcgcaggcgc tgctggcggg
     2701 cctgtcgggc gacgaacgcg cggcggtgat gggcgggacg gccgtcgagg tgtacgacct
     2761 ccggatcgac tgacggctcg cgacggacgt ccatccggac ctgtcgcatg gccgccccaa
     2821 tcgggccgaa cggcacacgg gcgcggaccg tcttcctccc taccgttgag ggcgcaagga
     2881 ggtcaaccat ggtccgcgcg tccggtcccg tctcgctccg cgacacctgg ttcggcgcgc
     2941 gtctgcccga ggaggcggtg acccggctcg agccgcacgt ccggcgagcc acgtacgacg
     3001 ccggcagcga gatcctgcgc gagggcgacc cgacctcggc gctgggcatc gtcgtgagcg
     3061 gccgcgttgc gctccgcctc cgtgtgcccg agcgcggccc gacgacggtc ctgaccgccg
     3121 agcccggcaa catcgtcggc tggtcggcgg tcgtcccgcc gcatcgggcg acctccacgg
     3181 ccgttgccct cgtcccgacg gagctgctgc tcctcgacgg cgaggcgctg cgatcccagc
     3241 tggatggcga ccccgtcctt gccgccgcgg tctatcgctc gctgctcgag gcggtcgcgc
     3301 accgcctcaa cggcacgcgc atgcagctgc tggacctgtt cgcgcagcag ggggacgagc
     3361 cgtggtgacc gcgaccatca ccccagcgtt cctggtccgc gcggacctcg gtcggctgtt
     3421 cgacctcctc cacgcggacg gccaccggat catcgggccg accgtacgcc agggcgccat
     3481 cgtgtacgcc gagatcgcga cgccagccga cctgcccacc ggctggacgg cgacccaggc
     3541 gcccgggaca taccggctgc agccgaccgg gacggaccgg gcgttcgact tcgcggtcgg
     3601 accgacgtcg tggaagcatg agacgtttcc gccgcgcatc ccgctgacgg tcgggcgccg
     3661 tgtcgacggc caggccacgt tcgaggacgc ggcacccgag gccccgcgag tcgcgttcct
     3721 cggcgtccgc ggctgcgaga tcgccgcctt gctgacgcag gacgccgtcc tggcgctcgg
     3781 tccggccgtc gatcgcgact acgtggcgcg acgggccgca
//
LOCUS       DBNHXO010000400         6364 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1702410, whole genome shotgun sequence.
ACCESSION   DBNHXO010000400 DBNHXO010000000
VERSION     DBNHXO010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1702410"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1629
                     /locus_tag="AC162A_08125"
     CDS             <1..1629
                     /locus_tag="AC162A_08125"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004676.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HIR5010846.1"
                     /translation="ARLVESARPGTDAQHIGSSAVPGMPGKNVVDLGIEADPDDIPDV
                     ADALISLGFQSQGGLAPFPPTRPLLLGNVDHGGTSFRIHLHVMPPARHELAELVAFRD
                     ALRSDASLRDGYAASKREFVEVAPDGEANLLYTVHKGDFVQDSLYRLGIRRPPADRPD
                     PLKPGSTIGILGGGQLGRMLGIAARAMGYRIVALDPDPECPTSAVADEIVVGAYDDAD
                     AARRLASKADVVTYELEHVGLEAATAAGEVAPLRPGLAALRATRDRLAERRFIREIGE
                     WAAPWREVRDVDDARAAADALGYPVRLKAAIGGYDGRSQVRITEPAEVEAAVAVLGGG
                     RAGPLLVEAEIDFEAELSVVCARDRDGRSLAFPLSRNVHDAGILVESAAPAPIHPLVA
                     ADAREIADSLVRGLDLVGLLTVELFQLRGGGLMINELAPRVHNSGHWTIEGAVTSQFE
                     QHLRAILGLPLGSVRPYGSAAMINLLGTGADRPARLQGLERALVDPDVHVHLYGKRRV
                     FERRKMGHVTVIASDVDEALERARGARAVLDWEG"
     gene            1631..2131
                     /gene="purE"
                     /locus_tag="AC162A_08130"
     CDS             1631..2131
                     /gene="purE"
                     /locus_tag="AC162A_08130"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR5010847.1"
                     /translation="MGDPGPLVGIVGGSRSDFPCLEKASMTLEQLGIGCELRVVSAHR
                     TPDHLFRYAETARGRGIRVIIAGAGGAAHLPGMLAAKTTLPIIGVPVPTEHLGGLDSL
                     LSIVQMPRGIPVATVAIGNAVNAALLAAQILGLSDEGIAGRVAAWREAQTDAVLDDPS
                     NAEPLG"
     gene            2154..2597
                     /locus_tag="AC162A_08135"
     CDS             2154..2597
                     /locus_tag="AC162A_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010848.1"
                     /translation="MDDERWEPGMPVLDRQPVRAPAGGPGASTLPPSLQGLPPRSVPE
                     TAPTPLQKHYVLLCAPALVAGFIAITALEVGAGFGSPLVKVCVLIAAPLLFVTTIDAT
                     LRIWRAAWAWMPVDRNKGLFRLAWVVVSLVFLALIVAATVLVLGA"
     gene            2599..3255
                     /locus_tag="AC162A_08140"
     CDS             2599..3255
                     /locus_tag="AC162A_08140"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5010849.1"
                     /translation="MERRLSENELAFLAMADRGPDGRSTDIASGDGTPDWITATLRYC
                     SRCGKPLAPAMLPEENRERLACAACGFIAYVNPRLVVTTLPVTDDGRLILLRRGIEPG
                     KGWWAQPGGFLEVDETVTEAAVRETLEETGLFVVPGEIVGLYARLEASVVVVAFEANV
                     AGGTMRTTPEALEVLPFAPADIPWTEIAFKTSFHAIRDWVRLRHPDAPQPGAFREPAE
                     "
     gene            complement(3451..4923)
                     /locus_tag="AC162A_08145"
     CDS             complement(3451..4923)
                     /locus_tag="AC162A_08145"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009341551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5010850.1"
                     /translation="MTTERAEPAAVAVSRMVIGGKLVAAADGRTFEVVEPHRGSVVAT
                     APLGDAVDVDRAVDAAQAAFDGDWGSMAASDRGRLLLRFAEVVRAHEDELATLESRQI
                     GKPISGARWEVGQVAKVLEFYAGATTKLRGSTIPVTRPGLDLTLREPIGVVGLIVPWN
                     FPIMMASWKVGPALAAGNTAVLKPASYSPLTALRLGELALEAGLPPGVLNVVTGPGPV
                     VGEAMASHPGIGKIAFTGETATGQEILRAAAGTVKKVSLELGGKSPNIVFADADIEAF
                     AAASPYAVFDNCGQDCCARSRIIVERSVHERVVELFAAATERVVVGDPSDPATEVGPL
                     VSFRQRERVEGYVESGLAEGATLVTGGVRPTDPALAEGAYLLPAVFDHVTPGMRIARE
                     EVFGPVVGVIPFDTDEEALRIANASPYGLSGSVWSRDIGRALRAAKALRSGVVSVNCN
                     NSVHVEAPFGGYKQSGIGKDLGLEALEQYTELKNVFIDLG"
     gene            5030..6082
                     /locus_tag="AC162A_08150"
     CDS             5030..6082
                     /locus_tag="AC162A_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR5010851.1"
                     /translation="MAGQRAVGAGSPWSRIARAFSEAIRGRHIRAWILAVLAVTAVGS
                     LGYMILFRWPPSDAVYMTVMTLTTVGFREVRPIDEPLARLWTMLLAVSGVAIIFGSIG
                     IVAETIVSDAASGKREAKRMREAVAALRGHYIVCGYGRVGSTVARELAHSGERLVVID
                     IEQASIERAARDGHLVVEGDATDDATLAKAGTEHARGLVTTIDSDANNVFVTLSARAM
                     NPSLFIVARANAEGTEAKLEQAGANRIVSPYTMAGRRIAELASRPRVADFIDAALSHG
                     NLSFSLEEVVVDTDSPLLGVTVGALRADGVVSLAILQRDGAYEANPPEDRALHAGESL
                     ITSGSTEALAALRARA"
     gene            complement(6135..>6364)
                     /locus_tag="AC162A_08155"
     CDS             complement(6135..>6364)
                     /locus_tag="AC162A_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010063338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010852.1"
                     /translation="DLDEHLLAIEKLFSPQLFIIGGGVSKRADQFVPRLTVRAEVIPA
                     ALRNDAGIVGAAMAAAEQEQARGQAQEQAPG"
BASE COUNT          840 a         2310 c         2317 g          897 t
ORIGIN      
        1 gcgcggctgg tcgagtcggc ccgacccggg accgacgccc agcacatcgg gagctcggcc
       61 gtgcccggga tgcccggcaa gaacgtcgtc gacctcggga tcgaggcgga cccggacgac
      121 atcccggatg tcgccgatgc cctcatctcg ctcggcttcc aaagccaggg cggcctcgcg
      181 ccgtttccgc cgacccggcc gctgctgctc ggcaacgtcg accatggcgg gacgtcgttc
      241 cgcatccacc tccacgtcat gcccccggcg cgccacgagc tcgccgaact ggtcgccttc
      301 cgcgacgccc tccgatcgga cgcctccctc cgtgacggct acgcggcgtc caagcgcgag
      361 ttcgtggagg tcgcgccgga cggcgaggcg aacctcctgt acacggtcca caagggcgac
      421 ttcgtccagg actcgctcta tcgcctcggc atccgccgac cgcctgccga ccggccggac
      481 ccgctcaagc ccggctcgac gatcgggatc ctgggtggcg gccagctggg tcgcatgctc
      541 gggatcgcgg cgcgtgccat gggttaccgg atcgtcgccc tcgacccgga ccccgaatgc
      601 ccgacatcgg cggtcgccga cgagatcgtg gtcggtgcct acgacgacgc cgatgccgcc
      661 cgtcgcctcg cgtcgaaggc cgacgtggtc acctacgagc tggagcacgt cgggctcgag
      721 gctgccactg cggccggcga ggttgccccg ctccggcccg gcctggccgc gcttcgggca
      781 actcgggaca gacttgccga gcggcggttc atccgcgaga tcggggagtg ggcggcgccg
      841 tggcgcgagg tccgggacgt cgatgatgcc cgcgccgccg cggacgcgct gggctacccg
      901 gtgcgactca aggcggcgat cggcgggtac gacgggcgca gccaggtccg catcaccgaa
      961 ccggccgagg tcgaggccgc ggtcgcggtc ctcgggggcg ggcgggccgg cccgctgctg
     1021 gtcgaagcgg agatcgactt cgaagccgag ctgtcggtcg tgtgcgctcg cgatcgcgac
     1081 gggcggtcac ttgccttccc cctgtcgcgc aacgtccacg acgccgggat cctggtcgag
     1141 agcgcggcgc ccgccccgat ccacccgctg gtcgcggcgg acgcccgtga gatcgcggac
     1201 agcctcgtgc gcggcctcga cctcgttggc ttgctcaccg tcgagctgtt ccagctccgg
     1261 ggtggcgggt tgatgatcaa cgagctcgcg ccgcgggtcc acaactcggg ccactggacg
     1321 atcgagggcg cggtaacgtc gcagttcgag cagcacctgc gagcgatcct ggggctgccg
     1381 cttggctccg tccgtccata cgggtcggcc gcgatgatca acctgctggg caccggggcg
     1441 gaccgcccgg cgcgactcca ggggctggag cgagccctcg tggacccgga cgtccacgtc
     1501 cacctctacg gcaagcgacg cgtgttcgag cggcgcaaga tgggccacgt gaccgtgatc
     1561 gcaagcgacg tggacgaggc gctggagcgg gcgcgcggcg cgcgggccgt gctggactgg
     1621 gagggctgag atgggcgatc cggggccgct cgtcggcatc gtcgggggca gccggtccga
     1681 cttcccgtgc ctcgagaagg cgtcgatgac cctcgaacag ctcggcatcg ggtgcgagct
     1741 ccgcgtggtg tcggcgcatc ggacgcccga ccacttgttc cggtacgcgg agacggcacg
     1801 ggggcggggg atccgggtga tcatcgccgg cgccggtggc gccgcccacc tgccgggcat
     1861 gctggccgcc aagaccacgc tgcccatcat cggcgtgccc gttccgaccg agcacctcgg
     1921 cggcctcgac tcgctgctct cgatcgtcca gatgccgcgc gggatcccgg tcgcgaccgt
     1981 cgccatcggc aacgcggtca acgcggcgct cctggcggcg cagatcctgg gcttgtcgga
     2041 cgaggggatc gccggacggg tcgcggcgtg gcgcgaggcg cagacggatg ccgtcctcga
     2101 cgacccgtcg aacgccgagc cgctggggta gtcccgcgta gactcggggg cagatggacg
     2161 acgagcgatg ggaaccgggg atgcccgttc tcgaccggca accggtgcgg gcgcccgcgg
     2221 gcggcccggg cgcctcgacg ctgccgccga gcctccaggg cctgcccccg cggagcgtcc
     2281 ccgaaaccgc tccaacgccg ctccagaagc actacgtcct gctgtgcgcc ccggcgctgg
     2341 tcgccgggtt catcgcgatc accgcgctcg aggtcggggc cgggttcggc agcccgctgg
     2401 tcaaggtgtg cgtgctgatt gccgcgccgc tgctgttcgt cacgaccatc gacgccaccc
     2461 tgcggatctg gcgcgccgcc tgggcctgga tgccggtcga ccgcaacaag ggcctgttcc
     2521 gcctggcctg ggtcgtcgtg agcctcgtgt tcctcgcgtt gatcgtcgcg gcgaccgtcc
     2581 tggtgctcgg agcctagggt ggagcgccgc ctgtccgaga acgagctcgc gttcctggcg
     2641 atggccgacc gggggcccga cggacgctcc acggatatcg cctcgggcga tggcaccccg
     2701 gactggatca cggcgacgct gcgctactgc tcgcgctgcg ggaagccact ggcgccggcg
     2761 atgctgcccg aggagaaccg cgagcggctg gcctgcgccg cgtgcggctt catcgcgtac
     2821 gtcaacccgc ggctcgtcgt gaccacgctg cccgtgaccg acgacgggcg gctaatcctg
     2881 ctgcggcggg gcatcgagcc gggcaaggga tggtgggcgc agccgggtgg cttcctcgag
     2941 gtggacgaga cggtcaccga ggccgcggtc cgcgagacgc tcgaggagac cgggctgttc
     3001 gtcgtgccgg gcgagatcgt ggggctgtac gcgaggctcg aagcgtccgt ggtggtcgtc
     3061 gcgttcgagg cgaacgtcgc cggcggcacg atgcgcacga cccccgaggc gctcgaggtg
     3121 ctgccgttcg cccccgcgga catcccctgg accgagatcg cgttcaagac ctcgttccac
     3181 gcgatccggg actgggtgcg gctgcgccat ccggacgcgc cgcaacccgg cgccttccgc
     3241 gagccggcgg agtagccgcg ggccagtgcc accggcgccg cttgcccggc aggccagcgg
     3301 atcggcctga agcgcagacc gcgacgcgcc tggaccccca cgctggcccg gcagacacgt
     3361 tcggggaccc tggtgcgctc gcgacgaggt cgcgaccggc ttcgcgtcgg ttcgctggcc
     3421 tcgcaggcca gcaccgggcg atccgcgtgc tcagccgagg tcgatgaaca cgttcttgag
     3481 ctcggtgtac tgctccagtg cctcgaggcc gaggtccttg ccgatccccg actgcttgta
     3541 gccgccgaac ggcgcctcga cgtggacgct gttgttgcag ttcaccgaga ccacgcccga
     3601 gcgcagcgcc ttcgcggcgc gcagtgcgcg cccgatgtcc cggctccaga cggaccccga
     3661 caggccgtag ggcgacgcgt tcgcgatccg gagcgcctcc tcgtcggtgt cgaacgggat
     3721 cacgccaacc acgggtccga acacctcctc gcgggcgatg cgcatcccgg gcgtcacgtg
     3781 gtcgaacacc gccggcagca ggtaggcgcc ctcggcgagc gcggggtccg tcgggcgcac
     3841 gcccccggtg accagcgtcg cgccctcggc caggccgctc tcgacgtacc cctcgacccg
     3901 ctcgcgctgg cggaacgaca ccagtggccc gacctccgtc gccgggtcgg acgggtcgcc
     3961 gaccacgacg cgctcggttg ccgcggcgaa cagctcgacg acacgctcgt gcaccgagcg
     4021 ctcgacgatg atccggctcc gcgcgcaaca gtcctggccg cagttgtcga acacggcgta
     4081 cggcgaggcc gcggcgaacg cctcgatgtc ggcgtcggcg aacacgatgt tggggctctt
     4141 gccgccgagc tcgaggctca ccttcttcac ggtccccgcc gcggcgcgca ggatctcctg
     4201 gcccgtcgcc gtctcgccgg tgaatgcgat cttgccgatc cccgggtgcg acgccatcgc
     4261 ctcgccgacc acgggcccgg gtccggtgac cacgttgagg accccgggcg gcagcccggc
     4321 ctcgagcgcc agctcgccca gccggagcgc cgtcagcggc gagtacgagg cgggcttgag
     4381 gaccgcggta ttgccggccg cgagcgcggg cccgaccttc cagctggcca tcatgatcgg
     4441 gaagttccag gggacgatga ggccgacgac accgatcggc tcgcgcaggg tcaggtcgag
     4501 gccgggtcgg gtgacgggga tcgtcgagcc gcgaagcttg gtcgtggcac ccgcgtagaa
     4561 ctcgagcacc ttggcgacct ggccgacctc ccagcgggcg ccgctgatgg gcttgccgat
     4621 ctggcggctc tcgagcgtcg ccagctcgtc ctcgtgcgcc cgcacgacct cagcgaagcg
     4681 cagcagcagc cggcctcgat cggacgccgc catcgagccc cagtcgccgt cgaacgcggc
     4741 ttgggcagcg tcgaccgccc ggtcaacgtc gaccgcgtca ccgagcggtg ccgtcgcgac
     4801 gaccgatcca cggtgcggct cgacgacctc gaacgtccgc ccgtccgcgg ccgcgacgag
     4861 cttcccgccg atcaccatcc ggctcaccgc gaccgccgcc ggctcggccc gttccgtggt
     4921 catggtgctc ctctcctcga gcgcgtgcgg ggtggctcgg tgggacgccg ccgtcgtccg
     4981 atcatacggg cacgtcggcg gacctcgccg ctgctgtagg ctcgtcgcga tggcaggaca
     5041 gcgggccgtc ggcgccggat caccgtggag ccgcatcgcg cgggccttct cggaggcgat
     5101 ccggggccgc cacatccggg cgtggatcct ggccgtgctc gccgtcacgg cggtcggcag
     5161 cctcgggtac atgatcctgt tccgctggcc gccgtccgat gccgtgtaca tgaccgtgat
     5221 gacgctcacc acggtggggt tccgcgaggt ccggccgatc gatgaacccc ttgcccggct
     5281 gtggacgatg ctgctggcgg tgtcgggcgt ggccatcatc ttcgggtcga tcgggatcgt
     5341 ggccgagacg atcgtgagcg acgcggcgag cggcaagcgg gaggcgaagc ggatgcgcga
     5401 ggcggtcgcg gccctgcggg ggcactacat cgtgtgcggg tacggccgcg tgggctcgac
     5461 ggtcgcgcgc gagctggcgc actccgggga gcgcctcgtc gtcatcgaca tcgagcaggc
     5521 gtcgatcgag cgcgcggcgc gcgacggcca cctcgtggtc gagggcgacg cgacggacga
     5581 cgcgaccctg gccaaggccg gcacggagca cgcccgtggc ctcgtgacca cgatcgactc
     5641 ggacgccaac aacgtgttcg tcaccctcag cgcccgcgcg atgaacccgt ccctgttcat
     5701 cgtcgcccgg gccaacgccg aggggaccga ggcgaagctc gagcaggcgg gcgccaaccg
     5761 gatcgtgtct ccttacacga tggccggccg gcgcatcgcg gagctcgcga gccggccacg
     5821 ggtcgcggac ttcatcgatg ccgcgctgtc gcacgggaac ctgtcgttct ccctggagga
     5881 ggtcgtggtc gacaccgaca gcccgctgct cggggtcacc gtgggtgcgc tgcgcgccga
     5941 cggtgtcgtc agcctggcga tcctgcagcg ggacggagcg tacgaggcga acccgcccga
     6001 agaccgggcg ctgcacgccg gcgagagcct gatcacgtcg gggtcaaccg aggcgctggc
     6061 ggcgctgcgc gcgagggcct gaccgaccgt cgcgccatcg cgccgtcgcg cccgagccgc
     6121 cgccgtggcc cgggtcagcc tggggcctgc tcctgcgcct gcccccgcgc ctgctcctgc
     6181 tcggcggcgg ccatcgccgc gccgacgatg cccgcgtcgt tgcggagggc cgccgggatc
     6241 acctccgcgc gcacggtgag ccgagggacg aactggtcgg cgcgcttgga cacgccaccg
     6301 ccgatgatga acagctgggg cgagaacagc ttctcgatcg ccagcaggtg ctcgtcgaga
     6361 tcgg
//
LOCUS       DBNHXO010000401         2319 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3091814, whole genome shotgun sequence.
ACCESSION   DBNHXO010000401 DBNHXO010000000
VERSION     DBNHXO010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3091814"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /gene="argS"
                     /locus_tag="AC162A_08160"
     CDS             <1..1196
                     /gene="argS"
                     /locus_tag="AC162A_08160"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR5010853.1"
                     /translation="SRVLEAGGQEVTREYYFNDAGTQISVLGASVVALRRHEPLPEEA
                     YRGAYVQDLADDLPDDVWAAAQAPGTDAAAVVGRWAATRVRANIEASLAHLGVRFDVW
                     KSEGSLHEEGWVARAVERLRDGGHVFEQDGALWFRSTDFGDDKDRVIYRSDGRPTYFA
                     ADIGYVTEKLGRGFDHLIYIWGADHHGTVARLRNAAQAMGYDRDAVEVLLYSWVRFVR
                     DGVEISMSKRAGEFITLDELLGEVGVDAARWFFGSRAATTGIDFDIELAKKQSAENPV
                     YYVQYAHARIASILRKGAEAGLTPAPDVAGLLAGEPEGLLVRAVSGFPEVVEDAVAAQ
                     ETHGITAYATELATQFHGFYRDARVVDVDQPERSAKRLALALAAKSTLANALGLLGIS
                     APEAM"
     gene            complement(1204..>2319)
                     /locus_tag="AC162A_08165"
     CDS             complement(1204..>2319)
                     /locus_tag="AC162A_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010854.1"
                     /translation="EIEGQVSALRGLTLTTPVERDVFNREELGTFIQGSFGRDNPESL
                     VASTETLLKALALMPQGDSLRDLYVEMLTSQVAGLYDDTTKSMYVISDTGEIGPLEEI
                     TYAHEYTHALQDQAFGLSKLHGDATDQGDRQLARTTLIEGDATLLMFLWAQQNLPPEQ
                     LLEVAGSSDPASQAVLDRLPALLKETLMFPYTTGLGLTQGQFVAAGGYAGIDALFADP
                     PDSTEQVLHPEKLNPREAPIEVTFDADLAAKLGAGWCVALQDTLGEFQLGIVLRDAGG
                     ADTSTANAAAAGWGGDRVALVGGPDGELGVMLDTRWDTAPDAGEFARALGQMAANLEA
                     AGRSVKVLTPAPDRVVLLVSNGDDTLGRLANVLGFPG"
BASE COUNT          326 a          813 c          841 g          339 t
ORIGIN      
        1 tgagccgggt cctcgaggcg ggcggccagg aggtcacgcg cgagtactac ttcaacgacg
       61 cggggaccca gatcagcgtc ctcggggcgt cggtcgtggc gctccggcgg cacgagccgc
      121 tgcccgagga ggcgtaccgc ggggcgtatg tccaggacct ggcggacgac ctgccggacg
      181 acgtgtgggc ggccgcgcag gcgcccggca cggacgcggc ggccgtcgtg ggccgctggg
      241 ccgcgacgcg ggtccgggcc aacatcgagg ccagcctcgc ccatctcggc gtgcgcttcg
      301 acgtctggaa gagcgagggc tcgctccacg aggaaggctg ggtcgcacgg gccgtcgagc
      361 gcctccgaga cggcgggcac gtgttcgagc aggacggcgc cctgtggttc cgctcgaccg
      421 acttcggtga cgacaaggac cgcgtgatct accgctcgga cggccggccc acctacttcg
      481 ccgccgacat cggctacgtc acggagaagc tgggccgcgg cttcgaccac ctcatctaca
      541 tctggggcgc ggatcaccac gggacggtgg cccggctccg caacgccgcc caggcgatgg
      601 gctacgaccg ggacgcggtc gaggtcctgc tgtactcgtg ggtccggttc gtccgcgacg
      661 gcgtggagat ctcgatgtcc aagcgcgccg gggagttcat caccctcgac gagctgctgg
      721 gcgaggtggg cgtcgacgcc gcccgctggt tcttcgggtc acgcgcggcg acgaccggga
      781 tcgacttcga catcgagctc gccaagaagc agtcggccga gaaccccgtc tactacgtcc
      841 agtacgcgca cgcgcgcatc gcgtcgatcc tgcgcaaggg cgccgaggcc ggcctgaccc
      901 cggctccgga cgttgccggc ctgctcgcgg gcgagccaga agggctgctc gttcgtgctg
      961 tgagcgggtt cccggaggtg gtcgaggacg cggtcgccgc ccaggagacc cacgggatca
     1021 ccgcgtacgc gaccgagctg gccacccagt tccacggctt ctaccgcgac gcgcgagtcg
     1081 tcgacgtcga ccaaccggag cgatccgcca agcggctcgc gctcgcgctg gccgcgaagt
     1141 ccacgctcgc caacgcgctg ggcctgctcg ggatctcggc gcccgaggcg atgtagccgc
     1201 gggtcagccg gggaagccca ggacgttcgc caggcggccg agcgtgtcgt cgccgttcga
     1261 gaccaggagc acgacccgat ccggggccgg cgtcagcacc ttgacgctgc gcccggccgc
     1321 ctccagattg gcggccatct ggccgagggc tctcgcgaac tcgccggcgt ccggggccgt
     1381 gtcccagcgg gtgtcgagca tgacgcccag ctccccgtcc ggaccaccga ccagggcgac
     1441 ccggtccccg ccccagcctg cggccgccgc gttggcggtc gaggtgtccg ctccgccggc
     1501 gtcacggagg acgatgccca gctggaactc gcccagcgtg tcctggagcg ccacgcacca
     1561 cccggcgccg agcttggcgg cgaggtcggc gtcgaaggtg acctcgatcg gtgcctctcg
     1621 ggggttgagc ttctcgggat gcagcacctg ctcggtcgag tcgggcgggt cggcgaacag
     1681 cgcatcgatc cccgcgtagc cgccggcagc aacgaactgt ccctgggtga gcccgagtcc
     1741 cgtcgtgtag gggaacatca gggtctcctt gagcagcgcg ggcagccggt ccaggacggc
     1801 ctgcgaagcg gggtcggacg agcccgcgac ctcgagcagc tgttccgggg gcaggttctg
     1861 ctgggcccac aggaacatga gcagcgtggc gtcgccctcg atcagcgtgg ttcgcgcgag
     1921 ctgccgatca ccctggtcgg tcgcatcgcc gtggagcttg ctcaggccga acgcctggtc
     1981 ctggagggcg tgcgtgtact cgtgcgcgta cgtgatctcc tcgagcgggc cgatctcgcc
     2041 cgtgtccgag atgacgtaca tgctcttggt cgtgtcgtcg tacaggcccg ccacctggct
     2101 ggtcagcatc tcgacgtaca ggtcgcgcag cgagtccccc tgcggcatca gggcgagcgc
     2161 cttgagcagg gtctccgtgc tcgcgacgag ggactcgggg ttgtcccggc cgaacgaacc
     2221 ctggatgaac gtgcccagct cctcgcggtt gaacacatcg cgctcgacgg gcgtcgtgag
     2281 cgtcaggccg cgcagcgccg acacctggcc ttcgatctc
//
LOCUS       DBNHXO010000402         1806 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3489353, whole genome shotgun sequence.
ACCESSION   DBNHXO010000402 DBNHXO010000000
VERSION     DBNHXO010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3489353"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1212)
                     /locus_tag="AC162A_08170"
     CDS             complement(187..1212)
                     /locus_tag="AC162A_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010855.1"
                     /translation="MTADDRWRAEIPVHAMLEDGLEARFVLSPGDRACVATGDVVKPG
                     DPLLEHLRDRRIDEVVIASGAGPGGDWAAGEQWSRSTGGRPRRDQPADGELLSIVPGQ
                     RDRWRIVVGDQRDTVDTPVGGQVVAVRPGGEVRLRAAGRALRGAFAAGVPAHGQLELA
                     TDPFGDLRPGGIDVGRAGSILVVGARIDAEALTRARAMGVRGIVVASLPGKDLRDFRA
                     SERRQRASLHPSQPFGVLVLDGTIRRAIPTPIAALLERIAGREVSLLVDPPALVFEAT
                     DDELPTVDEDWVRVRSGPRAGVEGRLVAPGGLRRFERGIQLESALVAIDGRAPVAIPI
                     GDLERIA"
     gene            complement(1209..>1806)
                     /locus_tag="AC162A_08175"
     CDS             complement(1209..>1806)
                     /locus_tag="AC162A_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010856.1"
                     /translation="VGRLLDATPTIDALPAPELVVVAGGAWNVAPPSAVALAVADVIR
                     RPGVRALGLDHARLLAPLGTIPDPAERRLIIVDLRDELVLPLGSVLMPAGMRTGRAAG
                     TLALRSTTGESLLDLVPGGLDLLHLPAGEVAIADVRFRDAVDLGPRVRHATVQVTGGL
                     AGLLVDLRDVPLRLPDRLEMRREAMASWEARVWAGSEA"
BASE COUNT          238 a          687 c          693 g          188 t
ORIGIN      
        1 ccggcgccga ggtcgcccac gaggcacagg aggtcgccgg ggcgggcgac ggtcgcgagc
       61 cgtgcggcca gggcacgggt ctcctcggcg tcgtgcgtcg cgatgacgcg ctcgcaaacg
      121 gcatctcggg gcatcgcccc gagggtagcc gaaccgcctg ccggacgccg gtggcccggg
      181 gccccctcag gcgatgcgct cgagatcgcc gatcgggatc gcgaccgggg ctcggccgtc
      241 gatcgccacc agcgccgact cgagctggat gccgcgctcg aagcgtcgca ggccgccggg
      301 cgcgaccagc cgtccttcga cgccggccct cgggcccgag cgcacgcgga cccagtcctc
      361 gtcgacggtc ggcagctcgt cgtccgtcgc ctcgaacacc agcgcgggcg ggtcaacgag
      421 caacgacacc tcgcggcccg cgatccgctc gagcagggcg gcgatcggcg tcgggatcgc
      481 gcgccggatc gtgccgtcga ggacgagcac gccgaacggc tgcgacggat gaagggaggc
      541 gcgctggcgc cgctcggagg cgcggaagtc gcgcaggtcc ttgccaggca gggatgcgac
      601 gacgatcccc cggacgccca tcgcgcgcgc ccgcgtgagg gcctcggcgt cgatgcgcgc
      661 accgaccacg aggatgctgc cggcccggcc cacatcgatg ccgcccggcc gcaggtcgcc
      721 gaacgggtcc gtcgcgagct cgagctggcc gtgggcgggt acgccggccg cgaacgcgcc
      781 gcggagcgcc cgtcccgccg cgcgcaggcg gacctctccg ccgggtcgca cggcgacgac
      841 ctggcccccg accggcgtgt cgacggtgtc acgctggtcg ccgacgacga tccgccagcg
      901 gtcgcgctgg ccggggacga tcgagagcag ctcaccatcc gccggctggt cccggcgcgg
      961 ccggccgccc gtcgaacgcg accactgctc cccggccgcc cagtcgccac ccgggccggc
     1021 tcccgatgcg atcaccacct cgtcgatccg gcggtcgcgg aggtgctcga gcagcgggtc
     1081 gccgggcttg acgacgtcgc cggtcgcgac gcacgcgcgg tcaccgggcg agagcacgaa
     1141 gcgggcctcg aggccgtcct cgagcatcgc gtgcacgggg atctcggcgc gccagcgatc
     1201 gtcagcggtc acgcctcgct ccctgcccac acgcgcgcct cccatgacgc catcgcctcc
     1261 cggcgcatct cgaggcggtc gggcagccgc agcgggacgt cgcgcaggtc gacgagcagg
     1321 ccggcgaggc ccccggtgac ctgcaccgtc gcatggcgca cgcgtggccc gaggtcgacc
     1381 gcgtcgcgga accgcacgtc ggcgatcgcc acctcgcccg cgggcaggtg caggaggtcc
     1441 agtccgccgg ggacgaggtc gaggagcgac tcgccggtcg tcgagcgcag tgcgagcgtg
     1501 cccgccgcac ggcccgtccg catgccggcg ggcatcagca cgctgcccag gggcaggacg
     1561 agctcgtcgc gcaggtcgac gatgatcagc cgccgttcgg cgggatcggg gatcgtgccg
     1621 aggggcgcga ggaggcgcgc gtggtcgagg ccgagcgcgc ggacgcccgg tcggcggatg
     1681 acgtccgcga cggccagcgc caccgccgag ggcggcgcga cgttccacgc gccaccggcg
     1741 acgaccacga gctcgggcgc cggcagggcg tcgatcgtcg gtgtggcatc gagcaggcga
     1801 ccgacg
//
LOCUS       DBNHXO010000403         2813 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2100619, whole genome shotgun sequence.
ACCESSION   DBNHXO010000403 DBNHXO010000000
VERSION     DBNHXO010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2100619"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="AC162A_08180"
     CDS             complement(<1..203)
                     /locus_tag="AC162A_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="High-affinity nickel-transporter"
                     /protein_id="HIR5010857.1"
                     /translation="MRSPRRSIAGGLLLGAILIPVVAGVALAHPLGNFTINHYAGIRV
                     EPSRVLLDVVIDEAEIPTFQARQA"
     gene            complement(200..1672)
                     /locus_tag="AC162A_08185"
     CDS             complement(200..1672)
                     /locus_tag="AC162A_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5010858.1"
                     /translation="MTVFTVRRPPRRPLIAVLGVLAFAIGTQVAAAWPSAGPVGLDEP
                     LLAAPPPAEAAAEPVADEPRAFPAIVPAPDLGPITIPAPGKVADVDPAAELARVRDDV
                     DFWAARLTARPADIVAAVKLAEADSAEARLTGDVTGYLRAEQAASAALEAQPSYVPAQ
                     AMRASILISLHRFPEARDLARSILDRSPGDATALGVLGDASLELGDLATASSAYSQLA
                     MVADGAASRVRSARLAFIQGDPAAAVAGDVAAVAAAVDEGLEGDALGFYDVTLGESLI
                     AAGDATAARSAFVAALDVRPGLPAALAGLAKLDAFDGDHSAAIAKLDDAIAAIPLPDW
                     LARRADLLTLRGDPGDARKAETDRATVEAIAQLAGEAGSVYDRGLSLYLSDRRLEPDR
                     AVRLARDELAVRADVYGYDTLAWALLNAGDAAGADAAMRSALTAGTRDARLWYHAGLI
                     ALANGRPAEAATSLQSALALGPALDPVARARAADAMATIR"
     gene            complement(1744..>2813)
                     /locus_tag="AC162A_08190"
     CDS             complement(1744..>2813)
                     /locus_tag="AC162A_08190"
                     /inference="COORDINATES: protein motif:HMM:NF025585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HIR5010859.1"
                     /translation="RVDITDVYAFKAGAGKTTLVLNVDGLMTPADSKVATYRANALYE
                     FKVDRNQDGTADLAYRVRFSPATTNADGTKTQAYVVRRETGAMAARNVWSGKKVAEGR
                     TTPYGHGVRTAHVKNGGSVFAGTRDDPFFFDLPGFVEFKNQLLGGSTDLGDLLGGFTA
                     ADTFAGTNVLSIAIKLPNARLGGTGNSIGVFATTSVPSNGGWMQVDRMARPAINTVFN
                     GLLLPSDSPYNGLEKDAFNLQRPSADAGTTTDNVTAVLNAIGNVLTANAATAYSAGEV
                     SAIAGVLLPDELTLTLGSGAPFASGSSLGTLALNGRGLSDDVIDAEFALLTNFVITTG
                     DGVGANDKAFKSAFPYLASPH"
BASE COUNT          404 a          934 c         1097 g          378 t
ORIGIN      
        1 gcctgccggg cctggaacgt cgggatctcg gcctcgtcga tgacgacgtc aaggagcacc
       61 cgcgacggct cgacgcggat cccggcgtag tggttgatgg tgaagttgcc gagcggatgc
      121 gccagcgcga cgcccgcgac gaccgggatc aggatggccc ccagcagaag gcctccggcg
      181 atggagcgac gaggcgacct catcggatcg tcgccatggc gtcggcggct cgcgcccgag
      241 cgaccggatc cagagcgggt cccagggcga gggcgctctg cagtgaggtc gcggcctcgg
      301 ccggccgtcc gttggcgagc gcgatcagcc cggcgtggta ccagagccgc gcgtcccggg
      361 tgccggcagt gagcgccgaa cgcatcgcgg catcggcgcc ggcggcatct ccggcgttga
      421 gcagggccca ggcgagtgtg tcgtagccgt agacgtcggc gcggacggcg agctcgtcac
      481 gagccagccg cacggcccga tccggctcca ggcgccggtc ggagaggtag agcgacagcc
      541 cgcggtcata gacggagccc gcctcgcccg cgagctgcgc aatcgcctcg acggtggccc
      601 ggtcggtctc ggccttgcgg gcatcgccgg ggtcgccgcg cagcgtcagc aggtcggcac
      661 gccgggcgag ccagtcgggc agcgggatcg cggcgatggc gtcgtcgagc ttcgcgatgg
      721 cggccgagtg atcgccgtcg aaagcatcga gcttggcgag gcctgccaac gcggcaggaa
      781 gcccggggcg gacgtcgagc gcggccacga acgccgaccg agcggccgtc gcatcgcccg
      841 cggcgatcag cgactcgcca agggtcacgt cgtagaagcc cagtgcgtcg ccttcgagcc
      901 cttcatcgac ggccgcagca acggcagcga catcgcccgc gaccgccgcc gccggatcgc
      961 cctggatgaa cgccagccgg gcggatcgga cccgggatgc ggctccgtcc gcgaccatcg
     1021 ccagctgcga ataggccgag gatgccgtgg ccaggtcccc cagctcgagc gacgcgtcgc
     1081 ccaggacgcc caacgccgtc gcgtcgcccg gcgagcggtc gaggatcgag cgcgcgaggt
     1141 cgcgcgcctc gggaaaccgg tggagcgaga tgaggatgct ggcccgcatc gcctgggccg
     1201 gcacgtacga aggctgtgcc tcgagggccg cggacgcggc ctgttccgcc cgcaggtagc
     1261 cggtgacgtc gccggtcagg cgcgcctccg ccgaatcggc ctcggcgagc ttcacggcgg
     1321 cgacgatgtc ggcggggcgc gcggtcagtc gggcggccca gaagtcgacg tcgtcgcgga
     1381 cccgggcgag ctcggcggcg gggtcgacgt cggcgacctt gcccggggcc gggatggtga
     1441 tgggaccgag gtcgggcgcg gggacaatgg ctgggaacgc acggggttcg tcagccaccg
     1501 gttcggccgc agcctcggcg gggggcggcg cggcgaggag cggctcatcg agaccgacgg
     1561 gaccggcgga tggccacgcc gcggcgacct gcgtcccgat cgcgaaggcc agcacgccga
     1621 gcacggcgat gagcggccta cggggcggtc ggcggacggt gaagacggtc atcggtgact
     1681 cctgcgcacg ggacttggag agccggggtg cgccgcccga tcgacggcgc accccggagg
     1741 gcctcagtgc ggcgaggcca ggtacgggaa ggcgctcttg aacgccttgt cgttcgcgcc
     1801 cacgccgtcg ccggtcgtga tcacgaagtt ggtgagcagg gcgaactccg catcgatcac
     1861 gtcgtcggac aggccgcgcc cgttgagggc gagcgtgccg agcgacgagc ccgaggcgaa
     1921 cggggcaccg ctgccgagcg tgagcgtcag ctcgtccggc aggagcacgc ccgcgatcgc
     1981 ggacacctca cccgccgaat aggccgtggc cgcgttcgcc gtcaggacat tcccgatcgc
     2041 gttgagcacg gccgtcacgt tgtcggtggt cgttccggcg tccgcggacg ggcgctggag
     2101 gttgaacgcg tccttctcga ggccgttgta cggcgagtcg gacggcagga gcagcccgtt
     2161 gaacacggtg ttgatcgccg gccgggccat gcggtcgacc tgcatccagc cgccgttcga
     2221 cgggacagag gtggtcgcga agacgccgat cgagttgccg gtgccgccga ggcgggcgtt
     2281 gggcagcttg atcgcgatcg agagcacgtt ggtgcccgcg aacgtgtcgg ctgcggtgaa
     2341 tccacccagc aggtcgccga ggtcggtgga gccgccgagc agctggttct tgaactcgac
     2401 gaacccggga agatcgaaga agaacgggtc gtcgcgggtg ccggcgaaca ccgagccgcc
     2461 gttcttgacg tgtgcggtgc gcacgccgtg cccgtagggg gtggtccggc cctcggcgac
     2521 cttcttgccg ctccacacgt tgcgggcggc catggcgccg gtctcgcggc gaacgacgta
     2581 cgcctgggtc ttcgtcccgt cggcgttcgt cgtggccggt gagaaccgga cgcgataggc
     2641 gaggtcggcc gtgccgtcct ggttgcggtc gaccttgaat tcgtacagcg cgttggcgcg
     2701 gtaggtggcg accttgctgt cggccggggt catgagcccg tccacgttga ggacgagcgt
     2761 ggtcttgccg gcgcccgcct tgaaggcgta gacgtcggtg atgtcgacgc ggt
//
LOCUS       DBNHXO010000404         5783 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2895972, whole genome shotgun sequence.
ACCESSION   DBNHXO010000404 DBNHXO010000000
VERSION     DBNHXO010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2895972"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="AC162A_08195"
     CDS             complement(<1..698)
                     /locus_tag="AC162A_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIR5010860.1"
                     /translation="MTLELIQGLLLAFALVVILMPPYMRLLRHLGFAKQIREEGPQSH
                     LAKGGTPTMGGLLVIVVVVIIFLVMRWPPQGGVIAPLATLAFVGLLGAADDYLNARTG
                     EGIRVRQKLLWQSVVALVVAYQIQNTYQITGIRVPFLGDVPIDPVVYIFFAAFAIVAS
                     SNGVNITDGLDGLAGGTLAFAFVSFLIIALLNVPAQPQIALFCALIIGALLGFLWFNV
                     HPAQVFMGDSGSLSL"
     gene            complement(695..2161)
                     /locus_tag="AC162A_08200"
     CDS             complement(695..2161)
                     /locus_tag="AC162A_08200"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR5010861.1"
                     /translation="MTDLGRSGDTPALTATDLLAAAGGRLLRRSDRPIRGGAVDSRLV
                     GPGMLFVALAGERTDGHRYLADAVAAGAAALIVGRLPDATAGEPAIDSLGDVTVVLVD
                     QPLRALHAVAAAWRRRFDPLVVGITGSIAKTSTKEAVAGVLERRFETLRTEGNQNNEV
                     GLPLTVLRLGPEHGAAVLEMGMYVGGEIRDLAAIGRPSIGIVTAIQPVHQSRIGSLEA
                     IVDAKAELLEALPSAADGGLAILNADDERVRPMAGRTSARTVTYGFADDADVRAVGVE
                     SRGLDGMRFRLVTPVGERTVEIAALGRLAVHNALAATAAGLAAGMTLDEVVPGLATPS
                     RAPHRSAVILAGGVVVVDDAYNAAPGSMLAALELLAGLPAEHRIAVLGEMRELGDAHD
                     AAHLEIGRAAGGALDTIVVVDGEPGGRARGIVVGALDAGMSADSVIAVADAAAAVSAA
                     AAVVRPGDAVLVKASRGVELERVVDGLIARLGGPETGT"
     gene            complement(2188..4017)
                     /locus_tag="AC162A_08205"
     CDS             complement(2188..4017)
                     /locus_tag="AC162A_08205"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR5010862.1"
                     /translation="MLGRTDSRARALLVLLAFVLVAGSLGMRLAYWQVSRRDDLSAMA
                     VRQSSMRYEIPSTRGAIFDRTGTVVLATSVSRDRLAAYPKLLSPERRAEVAARLVTIL
                     GLEGDAANELTARMLSERQYVILARDLDPALSDQIRADSEGEDPPLAALLLEPEPVRV
                     YPQPGGGPRTTLAAHILGFVNRDGQGQYGVEQFYQDQLAGMPRVVAAQKDAAGNPVPA
                     SSIVLDPGYAGQDLTLTLDASLQVAVEQELLAAWIADRAKRVSAVVMDPYTGEVVAYA
                     SYPSYDANDYQAIAGLAPERFVDPIVSTVYEPGSVFKMLTATAAIGRGTVTMQTRLND
                     TGKLWVDRGRAHVDDADHYAMGRMAFKDAVAYSRNVVAAKVALKLGHNTRESSRILYA
                     TWRKMGFGTNTGIDVANEVPGIVRDPAVTPWREIDLANGAFGQGIAVTPIQLAQAYAA
                     MVNGGTLVRPRVVRSIGDVETHPIYHGRVMTGGLSDTLQRLMKHVIAKVDFYRTRTLI
                     PGFEVGGKTGTAQIWDADKQAWKVNKFNYSFVGYIGRKTGHPDLVVAVRIEEGTPTVV
                     RLGHLEMPVMSFELFRRIAHDSINTPFLVPEDARVPADAADGP"
     gene            complement(4033..4416)
                     /locus_tag="AC162A_08210"
     CDS             complement(4033..4416)
                     /locus_tag="AC162A_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010863.1"
                     /translation="MAVYQGARQRTIVLPRRPRAEAQAPAPPRRRVRSAVRAHRGPSR
                     LALLLAAIVIAFAGAFFSLSQDIRLSATGYEMDRLATEQQRLQAQADDLRNELNRLGK
                     APAIRKFAIDAGLGPLPEPEVIPAR"
     gene            complement(4418..4615)
                     /locus_tag="AC162A_08215"
     CDS             complement(4418..4615)
                     /locus_tag="AC162A_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010864.1"
                     /translation="MSKRHHSNRRKNYGRRQHELHERSQRDHQPVEAEHALDEAGTPG
                     MTDRLSFMDSRSSRLRYALGD"
     gene            complement(4733..5677)
                     /gene="rsmH"
                     /locus_tag="AC162A_08220"
     CDS             complement(4733..5677)
                     /gene="rsmH"
                     /locus_tag="AC162A_08220"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR5010865.1"
                     /translation="MELGHLPVMPVEVLGTLAPAPGSLQIDATIGGGGHTERILEAAS
                     PDGRVLGLDADQAAIERVALRLARYGDRVVLRQANFRELASVAPAAGFPAVDGALFDL
                     GLSSFQLADRDRGFGFRTGGPLDMRFDTTRGVPASELLATLDADELAALFRKYGEEPS
                     AWKIAKAIVAARSTAPVETAEDLAQLVETVAPGNPRQRRRIHPATRVFQALRIAVNEE
                     LDALAEGLAAVVDLLRPGGRLVVLSYHSLEDRIVKRFIAAERRGCVCPPEAPVCVCGH
                     SPRLRLVTRPSMTPTDAEIDANPRARSARLRAAERLAA"
BASE COUNT          833 a         2018 c         2169 g          763 t
ORIGIN      
        1 agggacagcg agcccgagtc gcccatgaac acctgggccg ggtggacgtt gaaccacagg
       61 aacccgagca gcgcgccgat gatcagcgca cagaacagcg cgatctgcgg ctgggccggc
      121 acgttgagca gcgcgatgat caggaacgag acgaacgcga acgcgagtgt cccgcccgcg
      181 aggccgtcga gcccgtcggt gatattgacg ccgttcgacg aggcgacgat cgcgaacgcc
      241 gcgaagaaga tgtagacgac ggggtcgatg ggcacgtcgc ccaggaacgg gacccggatg
      301 ccggtgatct ggtaggtgtt ctggatctgg taggcgacca cgagcgccac cacggactgc
      361 cacagcagct tctggcggac ccggattccc tcgcccgtcc tggcgttgag gtagtcgtcg
      421 gccgcgccga gcaggccgac gaacgcgagc gtcgccagcg gcgcgatgac gccgccctgg
      481 ggcggccagc gcatgaccag gaagatgatc acgaccacga cgatcacgag cagcccgccc
      541 atcgtcggcg tcccgccctt ggcaaggtgg ctctgcggcc cctcctcccg gatctgcttc
      601 gcgaagccga ggtggcgcag cagccgcatg tatggcggca tcaggatcac gacgagcgcg
      661 aacgccagca gcagcccctg gatcagctcg agggtcacgt cccggtctcc ggcccgccca
      721 ggcgggcgat cagcccgtcg accacgcgtt cgagctcgac gccgcgcgac gccttgacga
      781 ggaccgcgtc gcccggacgc acgacggcgg ccgccgcgct gacggcggcc gcggcgtccg
      841 cgacggcgat gaccgagtcc gcggacatgc cggcgtcgag cgcgccgacg acgatcccgc
      901 gcgcccggcc gcccggctcg ccgtcgacga cgacgatcgt gtcgagcgcg ccaccggcgg
      961 cccggccgat ctcgaggtgg gccgcgtcgt gggcatcgcc cagttcgcgc atctcgccca
     1021 gcacggcgat gcggtgctcg gcggggaggc cggccaggag ctcgagggcc gcgagcatcg
     1081 agcccggggc ggcgttgtag gcgtcgtcca cgacgacgac gccgccggcg aggatgaccg
     1141 ccgaccggtg cggcgcccgt gatggcgtcg cgagcccggg gacgacctca tcgagggtca
     1201 tgccggcggc cagcccggcc gcggtggcgg ccagcgcgtt gtggacggcg aggcgcccga
     1261 gggccgcgat ctccacggtt cgttcgccga cgggcgtgac gagccggaaa cgcatcccgt
     1321 cgagtccccg cgattcgaca ccgacggcgc ggacgtcggc gtcgtcggcg aagccgtagg
     1381 tgaccgtccg ggcgctcgtc cggccggcca tcgggcgcac gcgctcgtcg tccgcgttga
     1441 ggatcgcaag cccgccgtcg gcggccgacg gcagcgcctc gagcagctcg gccttggcgt
     1501 caacgatcgc ctccagcgac ccgatccgcg actggtggac cggctggatc gcggtcacga
     1561 tgccgatcga cggtcgcccg atcgccgcca ggtcgcggat ctcgcccccg acgtacatcc
     1621 ccatctcgag gaccgcggcg ccatgctcgg gcccgaggcg gaggaccgtg aggggcaggc
     1681 cgacctcgtt gttctggttg ccctcggtcc gcagcgtctc gaaccggcgc tcgagcaccc
     1741 ccgcgacggc ctccttggtc gaggtcttgg cgatgctgcc ggtgatcccg acgacgagcg
     1801 ggtcgaaccg gcgccgccag gcggccgcca cggcgtgcag cgcccggagc ggttgatcaa
     1861 cgagcacgac ggtgacgtcg cccagcgagt cgatggccgg ctcaccggcg gtggcgtccg
     1921 ggaggcgtcc gacgatcagc gccgccgcgc ccgccgcgac cgcgtcggcc agataccggt
     1981 ggccgtcggt gcgctcgccg gccagggcga cgaacagcat gccgggcccc accaggcgcg
     2041 agtcgaccgc gcccccgcgg atcgggcggt cgctgcgccg cagcagcctg ccgccggcgg
     2101 cggcaaggag gtcggtggcg gtgagcgcgg gggtgtcgcc cgaccgtccg aggtccgtca
     2161 cggtcgcgag tgtcgcatag gccgagctca cggaccgtcc gccgcgtcgg ccgggacccg
     2221 cgcatcctcg ggcaccagga acggcgtatt gatcgagtcg tgggcgatcc ggcggaacag
     2281 ctcgaacgac atcacgggca tctccaggtg gccaagccgg accaccgtcg gcgtgccctc
     2341 ctcgatgcgc acggcgacga cgaggtcggg atgcccggtc ttgcggccga tgtacccgac
     2401 gaacgagtag ttgaatttgt tgaccttcca ggcctgcttg tcggcgtccc agatctgtgc
     2461 cgtgccggtc ttgccgccga cctcgaagcc cgggatcagc gtccgcgtgc ggtagaagtc
     2521 gaccttcgcg atcacgtgct tcatcagccg ctggagcgtg tccgagagcc cgccggtcat
     2581 cacccgcccg tggtagatcg ggtgggtctc gacatcgccg atggagcgga ccacgcgcgg
     2641 ccggacgagc gtcccgccgt tgaccatcgc cgcgtacgcc tgcgcgagct gaatcggcgt
     2701 gaccgcgatc ccctgcccga acgcgccgtt ggcgagatcg atctcgcgcc acggggtcac
     2761 ggccgggtcg cggacgatgc ccggcacctc gttggcgacg tcgatcccgg tattcgtccc
     2821 gaaccccatc ttgcgccacg tggcgtacag gatccgcgac gactcgcggg tgttgtggcc
     2881 gagcttgagc gccaccttcg ccgccacgac gttgcgcgag taggcgacgg cgtccttgaa
     2941 cgccatccgg cccatcgcgt agtggtcggc gtcgtcgacg tgcgcccgac cgcgatcgac
     3001 ccagagcttg ccggtgtcgt tgaggcgcgt ctgcatcgtg acggtcccgc ggccgatggc
     3061 cgccgtcgcg gtgagcatct tgaacaccga gccgggttcg tagacggtcg acacgatcgg
     3121 gtcgacgaag cgctcggggg ccaggccggc gatcgcctgg tagtcgttcg cgtcgtacga
     3181 ggggtagctc gcgtacgcga caacctcgcc cgtgtagggg tccatcacga ccgccgagac
     3241 gcgcttggcg cggtcggcga tccatgccgc caggagctcc tgttcgaccg cgacctggag
     3301 cgaggcgtcg agggtgagcg tgaggtcctg gccagcgtag ccggggtcga gcacgatcga
     3361 cgaggccggc accgggttgc cggcggcgtc cttctgggcg gccaccaccc ggggcatccc
     3421 ggcgagctgg tcctggtaga actgctcgac gccgtactgg ccctggccgt cgcggttgac
     3481 gaagccgagg atgtgggccg cgagcgtggt ccgcggaccg ccgccaggct ggggatagac
     3541 ccgcaccggc tccggctcca gcagcagcgc ggcgagcggc gggtcctcgc cctcgctatc
     3601 ggcgcggatc tgatcggaca gcgccgggtc gaggtcccgc gcaaggatga cgtactgacg
     3661 ctcggacagc atccgcgcgg tcagctcgtt cgcagcgtcg ccttcgagcc cgaggatcgt
     3721 gacgagccgc gccgcgacct ccgcccgccg ctcgggcgac aggagcttgg gatacgcggc
     3781 cagccgatcg cgcgagacgc tggtggccag gacgacggtg cccgtccggt caaagatggc
     3841 gccccgggtc gacgggatct cgtagcgcat cgacgactgt cggaccgcca tcgccgacag
     3901 gtcgtcgcgg cgcgagacct gccagtacgc gaggcgcatg ccgaggctgc ccgccaccag
     3961 cacgaaggcc aggaggacga gcagcgcgcg ggcgcgggaa tcggtccggc caagcacgac
     4021 gatccggcgc ccctagcgcg ccgggatgac ttcgggttcc gggagcggcc cgaggccggc
     4081 gtcgatcgcg aacttgcgga tcgccggcgc cttgccgagt cggttgagct cgttgcgcag
     4141 gtcgtcggcc tgggcctgga gacgctgctg ctcggttgcg agccggtcca tctcgtagcc
     4201 cgtggccgag agccggatgt cctgggacag cgagaagaac gccccggcga acgcgatcac
     4261 gatcgccgcc agcaggagcg cgagccggga cgggccacga tgcgcgcgga ccgccgaacg
     4321 gacgcggcgg cgcggcgggg ccggcgcctg cgcctcggca cggggccgac gcggcaggac
     4381 gatggtgcgc tgccgcgccc cctggtagac ggccacgtca atcgccgagc gcgtagcgga
     4441 ggcgggacga gcgggaatcc atgaaggaga ggcggtcggt catgccgggc gtgccggcct
     4501 cgtcgagggc gtgctcggcc tcgacgggct ggtggtcacg ctggctgcgc tcgtgcagct
     4561 cgtgctggcg gcggccgtag ttcttgcgac ggttcgaatg atggcgcttg ctcacgggac
     4621 tcctcctccc aaagcgcggg ccggcgtggg cgccggctgg atcgaccggg cgggcagtga
     4681 ccggtcggcg ggtgtgtcgg cgaacgcggt acgtcagtcg atagctgcgg gctcaggcgg
     4741 ccaggcgctc ggcggcgcgc aggcgcgcgc tccgggcccg ggggttggcg tcgatctcgg
     4801 cgtcggtggg cgtcatcgag ggccgggtca cgaggcgcag gcgcggcgag tgcccgcaga
     4861 cgcagaccgg ggcctcgggc ggacacacac agccgcggcg ttcggcagcg atgaaccgct
     4921 tgacgatgcg atcctcgagc gagtggtagg acaggacgac aaggcgtccg ccgggacgca
     4981 acaggtcgac gaccgccgcc agaccctcgg ccagcgcgtc gagctcctcg ttgaccgcga
     5041 tgcgcagggc ctggaacacg cgcgtcgccg ggtggatgcg gcgccgctgg cgcgggttgc
     5101 ccggggcgac ggtctcgacg agctgcgcga ggtcctcggc ggtctctacc ggagcggttg
     5161 aacgcgcggc gacgatggcc ttggcgatct tccaggcgga cggctcttcg ccgtacttgc
     5221 ggaacagggc ggccagctcg tcggcgtcga gcgtcgcgag cagctcggac gcgggcacgc
     5281 ctcgcgtcgt gtcgaagcgc atgtccagcg ggccgccggt ccggaagccg aagccccgat
     5341 cgcgatcggc caactggaac gacgagagcc cgaggtcgaa cagggcgccg tcgacggcag
     5401 gaaagccggc cgcgggggcg acgctcgcga gctcgcggaa gttcgcctgc cggagcacga
     5461 cccggtcgcc gtaccgtgcg aggcgcagcg cgacccgctc gatcgcggcc tggtccgcgt
     5521 ccagccccag cacgcggccg tcggggctgg cggcctccag gatccgctcg gtgtgcccac
     5581 cgccgccgat cgtcgcgtcg atctggagac tgccgggcgc cggtgcgagc gtcccaagga
     5641 cctcgaccgg catgaccggc aggtgcccta gctccatctc tcctccacga gtcggttgcg
     5701 gatcccgagc ggaccctgga agcgcatgtg gtgcgacgtc agatccccag tccggcgatc
     5761 gcgcgggcga acgcgtcggg gtc
//
LOCUS       DBNHXO010000405         1788 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3491301, whole genome shotgun sequence.
ACCESSION   DBNHXO010000405 DBNHXO010000000
VERSION     DBNHXO010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3491301"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="AC162A_08225"
     CDS             <1..1077
                     /locus_tag="AC162A_08225"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HIR5010866.1"
                     /translation="GLEILVEQGAGAGAAIPDQAFADAGATVVPTDALYAQSDVVLKV
                     QKPGPAEVGRLRKGQALVGFLQPLIDPAGAKALADAGVTAISLDAIPRTLSRAQTMDA
                     LSSQANVGGYKAVLIAANAYPRYFPLLTTAAGTAKPANVLILGIGVAGLQAIGTAKRL
                     GAVVHAYDVRPETREQAESLGAKFVKLKTTIDATGAGGYARELTAEERAAQQAELNEA
                     IGGMDVVITTAQVPGRKPPVLVTAAAVEQMKPGSVIVDMAASTLGGNCELSRVGETVE
                     TPNAVTIIAPDNLPASMPAGASAFYARNISALLLGMVKDGALHLDFEDEVTKSTVITR
                     DGEILSDAVRKLLEPAPSPAGGPA"
     gene            1074..1406
                     /locus_tag="AC162A_08230"
     CDS             1074..1406
                     /locus_tag="AC162A_08230"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010620189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha part 2"
                     /protein_id="HIR5010867.1"
                     /translation="MSQELLTSLAIFTLAILVGFEVISKVPATLHTPLMSGANSIHGI
                     VLVGAMIIASEADNPVSYVLAFVAMMFGAMNVVGGYVVTDRMLQMFRKKPTAAKAGTE
                     GASDAPGA"
     gene            1421..>1788
                     /locus_tag="AC162A_08235"
     CDS             1421..>1788
                     /locus_tag="AC162A_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIR5010868.1"
                     /translation="MSSEWIYTFVYLAWLVGASCFVLGLHRMNSPATGRSGNQLSAAG
                     MVIAVVATFVWLLTREGGLSGIALAIIVVGFLIGGGAGLYTARSVKMTAMPQLVSLFN
                     AVGGGAAALVAIDDFIRLSNG"
BASE COUNT          263 a          645 c          615 g          265 t
ORIGIN      
        1 gggctggaga tcctcgtcga gcaaggtgcc ggagccggtg ccgccatccc cgaccaggcg
       61 ttcgccgacg ccggcgcgac ggtcgtcccc acggacgctc tctacgcgca gtcggatgtc
      121 gtgcttaagg tccagaagcc cggcccggcc gaggtcggcc ggctgcgcaa gggccaggcg
      181 ctggtcgggt tcctccagcc gctgatcgat ccggccggcg cgaaggcgct cgccgatgct
      241 ggcgtgaccg cgatcagcct cgacgcgatc ccgcggacgc tgagccgtgc gcagaccatg
      301 gacgcgctct cgtcgcaggc gaacgtcggc ggctacaagg cggtcctcat cgccgccaac
      361 gcctacccgc gctacttccc cctgttgacc accgccgcgg gcaccgcgaa gcccgccaac
      421 gtcctgatcc tgggcatcgg cgtcgccggc ctgcaggcga tcggcacggc gaagcgcctc
      481 ggggccgtcg tgcacgcgta tgacgtccga cctgagacgc gcgagcaggc ggagagcctg
      541 ggcgccaagt tcgtcaagct caagacgacc atcgacgcga ccggcgccgg cggctacgcc
      601 cgcgagttga ccgccgagga gcgggccgcc cagcaggccg agctgaacga ggcgatcggc
      661 ggcatggacg tcgtgatcac cacggcccag gtgcccggcc gcaagccgcc ggtgctcgtc
      721 accgcggccg ccgtcgagca gatgaagccg ggctcggtga tcgtcgacat ggcggcgtcc
      781 acgctcggtg gcaactgcga gctgtccagg gtcggcgaga ccgtcgagac gcccaacgcg
      841 gtcacgatca tcgcgcccga caacctcccg gcatcgatgc ccgccggcgc atccgcgttc
      901 tacgcgcgga acatctcggc cctgctgctc gggatggtca aggacggggc gctccacctc
      961 gacttcgagg acgaggtcac gaagtccacg gtcatcaccc gcgacggcga gatcctctcg
     1021 gacgccgtgc gcaagctgct ggagccggcg ccatcgcccg caggaggccc cgcgtgagcc
     1081 aggaactcct cacctcgctg gcgatcttca cgctggcgat cctcgtcggg ttcgaggtca
     1141 tcagcaaggt cccggcgacc ctgcacaccc cgctgatgtc cggggcgaac tcgatccacg
     1201 gcatcgtcct cgtgggcgcc atgatcatcg cgtccgaggc cgacaacccg gtcagctacg
     1261 tcctcgcgtt cgtcgcgatg atgttcgggg cgatgaacgt cgtcggcggc tacgtcgtca
     1321 ccgaccggat gctccagatg ttccgcaaga agccgacggc cgccaaggcc gggaccgagg
     1381 gcgcgtcgga cgcccccggg gcctagtcga ggagccggtc gtgtcctctg agtggatcta
     1441 caccttcgtc tacctcgcct ggctggtggg ggcctcgtgc ttcgtgctgg gcctccaccg
     1501 catgaactcg cccgcgaccg ggcgcagcgg caaccagctg agcgccgcgg gcatggtcat
     1561 tgccgtcgtg gcgacgttcg tctggctgct cacccgtgaa ggcgggctgt ccgggatcgc
     1621 gctcgcgatc atcgtcgtcg gcttcctgat cggcgggggc gcgggcctgt acaccgcgcg
     1681 cagcgtgaag atgaccgcga tgccgcagct ggtgtcgctg ttcaacgctg tcggcggcgg
     1741 cgcggcggcc ctggtcgcca tcgacgactt catccgcctg tccaacgg
//
LOCUS       DBNHXO010000406         1525 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_119371, whole genome shotgun sequence.
ACCESSION   DBNHXO010000406 DBNHXO010000000
VERSION     DBNHXO010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_119371"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="AC162A_08240"
     CDS             complement(<1..499)
                     /locus_tag="AC162A_08240"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR5010869.1"
                     /translation="MLRTRALSALVLVPVLLVALYLGVQAIALVLALVAGLAGMEVYR
                     LLRAAGYPSLSIFGSVLAVAIVLQAGFAPEPEKGLVLIGLGAILAGAGSLVRLDPRDG
                     LAAWITTVFGALYIGLIAFVLYVAWMAPPVSQSAPLATLGAQRGWILLLVLGVWSYDT
                     GAYLVG"
     gene            complement(511..1320)
                     /gene="uppS"
                     /locus_tag="AC162A_08245"
     CDS             complement(511..1320)
                     /gene="uppS"
                     /locus_tag="AC162A_08245"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HIR5010870.1"
                     /translation="MAQAPLDRSLARPTDAPPLAHEGPGESVEDVAPAADAPLLPPEE
                     LPRHVAIIMDGNRRWARARDKNDFEGHAAGVEAIRELLKHAVRRGIPMLTLYAFSREN
                     WARSDEEVAGLFDLLGQAIHDETDELRAQGVRVRLLGRLEELPTATRSSIGDALDRTS
                     DGTRLQLNIAWNYAGRTELVDAFRRIAAAGTPADAIDERTISDALYTGGLPDPDLVIR
                     TGGEQRISNFLIWQSAYAELVFTECLWPDFGPGAFDEALLEFARRTRRFGR"
     gene            complement(1320..>1525)
                     /locus_tag="AC162A_08250"
     CDS             complement(1320..>1525)
                     /locus_tag="AC162A_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013891.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR5010871.1"
                     /translation="RRMEEARVEIRNLRREAADALKKEERDGEVGTDESRRQLDQLQK
                     ITDRVVADVDRLGDVKEQEVLEV"
BASE COUNT          219 a          535 c          568 g          203 t
ORIGIN      
        1 tcccgacgag gtaggcgccg gtgtcgtagc tccatacccc gagtacgagc agcaggatcc
       61 agccgcgctg ggcgccgagg gtggcgagcg gcgccgactg cgagaccggc ggtgccatcc
      121 aggccacgta caggacgaag gcgatcaggc cgatgtacag cgcgccgaag acggtcgtga
      181 tccacgccgc caggccgtcg cgcggatcga ggcggacgag gctgccggct cccgcgagga
      241 tcgcgcccag cccgatcagg accaggccct tctcgggctc cggggcgaac ccggcctgga
      301 ggacgatggc cacggcgagc acgctgccga agatcgacag ggacgggtag cccgccgctc
      361 gcagcagccg gtagacctcc atgcccgcga ggcccgcgac gagcgcgagg acgagggcga
      421 tcgcctggac accgaggtag agcgcgacca gcaggacggg gacgaggacg agcgccgaga
      481 gagcccgggt tcgaagcacc ggggcggccg ctagcgtccg aaccggcgcg tgcggcgcgc
      541 gaactcgagc agcgcctcgt cgaacgcgcc ggggccgaag tcgggccaca ggcactcggt
      601 gaacacaagc tcggcgtagg cggactgcca gatcaggaag ttgctgatcc gctgttcgcc
      661 accggtacgg atgacgaggt ccgggtccgg caggccgccc gtgtacagcg cgtcgctgat
      721 ggtgcgctcg tcgatcgcgt cggcgggcgt gcccgcggcc gcgatgcgcc ggaacgcgtc
      781 aacgagctcg gtccggcccg cgtagttcca ggcgatgttc agctggagcc gggtgccatc
      841 cgaggtgcgg tcgagggcgt cgccgatcga gctccgggtc gccgtcggca gctcctcgag
      901 gcggccgagg aggcgcacgc ggacgccctg tgcgcgcagc tcgtcggtct cgtcgtggat
      961 cgcctggccg agcaggtcga acaggccggc gacctcctcg tcggagcggg cccagttctc
     1021 gcggctgaac gcgtacagcg tgagcatcgg gatcccgcgc cggaccgcgt gcttgagcag
     1081 ctcgcggatc gcctccacgc cagcggcgtg gccctcgaag tcgttcttgt cgcgcgcccg
     1141 cgcccagcgg cggttgccgt ccatgatgat cgccacgtgg cggggcagtt cctccggcgg
     1201 caggagtggc gcgtcggcgg cgggcgcgac gtcctcgacc gactcgcccg gcccctcgtg
     1261 cgcgagcggt ggggcgtcgg tcggcctggc cagcgaacga tcgagcggcg cctgggccac
     1321 tagacctcga ggacctcctg ctccttgaca tcccccaggc gatcgacgtc tgcgaccacc
     1381 cggtccgtga tcttctgaag ctggtccagc tggcgccggg actcgtcggt cccgacctcg
     1441 ccgtcccgct cctccttctt gagggcgtcg gcggcctcgc ggcggagatt gcggatctcg
     1501 acgcgggcct cctccatccg cctgt
//
LOCUS       DBNHXO010000407         2003 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_517041, whole genome shotgun sequence.
ACCESSION   DBNHXO010000407 DBNHXO010000000
VERSION     DBNHXO010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_517041"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1985)
                     /locus_tag="AC162A_08255"
     CDS             complement(<1..1985)
                     /locus_tag="AC162A_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5010872.1"
                     /translation="MKRLVSALAVGCLLLGLAPTALAAGPTRHFERVDRNLDKIDPEF
                     RPMLADRTRKITAVLELKAQPALAREASSADRKIKAQTLRATQGRLDKRIDAIGAKVL
                     HRYQYAYNGIKVRATGAQLRRLAAMPGVVGVRRLATYKTDNVRAVPYVGAPTAWQATG
                     VTGAGQTIAVIDSGIDYTHANFGGPGTTEAYDDNDPTIIEDGTFPTTKVVAGYDFAGN
                     DYDGEGDAGSTTPTPDPDPLDCGDHGSHVAGTAAGQGVLADHSTYPGPYNSSTLSDPD
                     AFVVGPGVAPNAKLIALKVFGCEGSTDLVVDALEWVAGYNISHVDGIDVVNMSLGSVF
                     GRNGEPDVIATNNLVGIGVVVVASAGNSNAVPFITGSPAASTRAISVAALDAFPTIPM
                     ATVVLPTDQDIPGNNQNAYPALPVSGTLHVVPDGLGGVSLGCTADDYDAGSAGKIVAI
                     KRGVCAFVEKGAAAAEAGAIGIIDINRDDTAPGDLPTFLGYNPELFEIPMIGVDKTAQ
                     AALLANEGAAITLAPNGTTANPTYKQIADFSSSGPRYGDNWLKPDVAAPGVNMLSSLN
                     GSGWNGTTYSGTSMASPMTAGTAALVLSSHPTWSPLQVKAAIVNTANASSSKVLDYSP
                     LRSGSGVVQANRAVNTVGLATTSQGTASLSFGYETSKG"
BASE COUNT          259 a          641 c          763 g          340 t
ORIGIN      
        1 cccttggacg tctcgtagcc gaacgagagg ctcgcggtgc cctggctggt ggtggccagg
       61 cccacggtgt tgaccgcgcg gttggcctgg accacgcccg agccggagcg gagcgggctg
      121 tagtcgagca ccttggagct cgaggcgttc gccgtgttga cgatggcggc cttgacctgg
      181 agcggtgacc aggtcggatg ggacgagagc acgagcgcgg ccgtcccggc ggtcatcggc
      241 gacgccatgg acgtcccgga ataggtcgtc ccgttccagc cgctgccgtt gagcgaggac
      301 agcatgttga cgcccggcgc ggcgacgtcc ggcttcagcc agttgtcgcc gtagcgcgga
      361 ccggacgagc tgaagtccgc gatctgcttg taggtgggat tggcagtcgt gccgttgggt
      421 gcgagcgtga tcgccgcgcc ctcgttggcg agcagcgccg cctgcgccgt cttgtcgacc
      481 ccgatcatcg ggatctcgaa cagctcgggg ttgtagccga ggaacgtcgg caggtcgccc
      541 ggcgccgtgt cgtcgcggtt gatgtcgatg atcccgatcg cgccggcctc ggcggccgcc
      601 gccccctttt cgacgaacgc gcagacgccg cgcttgatcg cgacgatctt gcccgcgctg
      661 cccgcgtcgt agtcgtcagc cgtgcagccg agactcacgc cgccgagccc gtcggggacc
      721 acgtggaggg tgcccgacac cgggagggcc gggtaggcat tctggttgtt gccggggatg
      781 tcctggtcgg tcggcaggac gaccgtcgcc atcgggatcg tcgggaaggc atcgagcgca
      841 gcgacgctga tcgcccgcgt cgaggccgcc ggcgagccgg tgatgaacgg cacggcgttc
      901 gagttgcccg ccgatgccac cacgacgacg ccgatgccga cgaggttgtt ggtcgcgatc
      961 acgtcgggct cgccgttccg cccgaagacg gagccgagcg acatgttgac gacgtcgatg
     1021 ccgtcgacat gcgagatgtt gtacccggcg acccactcga gggcgtcgac gacgaggtcg
     1081 gtcgaccctt cgcagccgaa caccttgagc gcgatgagct tggcattggg agccacgccc
     1141 ggaccgacga cgaacgcgtc ggggtcgctg agggtcgagc tgttgtacgg gcccgggtag
     1201 gtgctgtggt cggcgaggac gccctggccg gccgcggtgc cggcgacgtg cgaaccgtgg
     1261 tcgccgcagt cgagcgggtc cgggtcgggc gtcggggtgg tcgacccggc gtcgccctcc
     1321 ccgtcgtagt cgttgccggc gaagtcgtag ccggcgacga ccttcgtggt cgggaaggtg
     1381 ccgtcctcga tgatggtcgg gtcgttgtcg tcgtaggcct cggtggtgcc ggggccgccg
     1441 aagttggcgt gcgtgtagtc gatgccgctg tcgatgacgg cgatcgtctg gccggcgccg
     1501 gtgacgcccg tggcctgcca cgcggtcgga gcgccgacgt acgggacggc cctgacgttg
     1561 tccgtcttgt acgtggcgag gcgccgcacg ccgacgacgc cgggcatcgc cgcgaggcgc
     1621 cgcagctggg cgccggtcgc gcggaccttg atgccgttgt aggcgtactg gtagcgatgg
     1681 aggaccttcg cgccaatcgc gtcgatccgc ttgtcgagcc tgccttgggt cgcgcgcagc
     1741 gtctgcgcct tgatcttgcg gtcagcggag ctggcctcgc gggccagggc cggctgggcc
     1801 ttgagctcga gcacggcggt gatcttgcgt gtcctgtcgg caagcatcgg ccggaactct
     1861 ggatcgatct tgtcgaggtt gcgatccacg cgttcgaagt ggcgggttgg cccggccgcg
     1921 agtgccgtgg gcgcgaggcc cagcagcagg cagccgacgg ccagggcgct gacgagtcgc
     1981 ttcatgcgct cccttcctcc aag
//
LOCUS       DBNHXO010000408         2719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5479102, whole genome shotgun sequence.
ACCESSION   DBNHXO010000408 DBNHXO010000000
VERSION     DBNHXO010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5479102"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1942)
                     /gene="glgP"
                     /locus_tag="AC162A_08260"
     CDS             complement(<1..1942)
                     /gene="glgP"
                     /locus_tag="AC162A_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HIR5010873.1"
                     /translation="MEPLVHVPVAPGASFHLPPKLEGLRTLAYNMWWSWHPRARIMFS
                     RIDSGGWARYRNPIPVLSGPVDWSLLLDNTDFMADYHDILAEFEAYMANGADHWFQRR
                     HAKELEGPIAYFCAEYGLHESLGIYSGGLGVLAGDHMKTASDMALPLIGVGLLYRKGY
                     FRQTIDADGHQEHAYPDYEPARLPIRRALDPLGEPLMVSVPLPGRELFAAVWVVQVGR
                     VPVLLLDTDVPDNDDSDRPITHILYVRGREMRLHQELVLGVGGVRALRQLDLDPAVWH
                     LNEGHSAFLLAERARELVASGKSLDDAFSMVRRNSVFTIHTPVSAGNERFDADLVRRV
                     AGPLLDGDGRPSTGGVPVERLLTMGLGVENDPSQFDMTALCLRTTHGANAVSLLHGNT
                     ANSTWQGISPHEILGLTNGVHTPTWLGQPMRDAIERFTNADMDAMDDQPASKRFWERI
                     AKVPPDEMWEAHQRQKLELAIFARGRLRNQFARHGEAPSALEELEGVLDPSILTIGFA
                     RRFATYKRAALLFSDLDRLARLLWDERRPMQVIFAGKAHPADRPGQGVIQDIFGRSRS
                     PKLRGRVFIVEDYDIRIARFLVQGVDVWLNNPRRPLEASGTSGMKAAANGVVNVSVLD
                     GWWDEGWTGDNGWAIGGRETDDD"
     gene            2032..>2719
                     /locus_tag="AC162A_08265"
     CDS             2032..>2719
                     /locus_tag="AC162A_08265"
                     /inference="COORDINATES: protein motif:HMM:NF019924.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HIR5010874.1"
                     /translation="MTMRVAMLAAECEPWAKTGGLADVVDALARALGRLPEAGTELAA
                     PVDVFLPRYRSVPVPDGALTEPTLTIPDPDRARRGAPIAVSIVNVAADGYRLRLVDVP
                     AAFDRDAFYDHPDDPWRFAVFCRAALAALRRDARPIDIVNVHDWHTGPALLERARADA
                     ARDAFFARTAVVATLHNLAYHGWTGNDRIGQLGLRAGEPLAGAHPDGIDLLLTAIDRS
                     EIANTVSPGFA"
BASE COUNT          370 a          943 c         1011 g          395 t
ORIGIN      
        1 cgtcgtcgtc ggtctcgcgg ccgccgatcg cccagccgtt gtcgccggtc cagccctcgt
       61 cccaccagcc gtcgagcacg ctgacgttga cgacgccgtt ggccgcggcc ttcatgcccg
      121 acgtccctga ggcctcgagc ggccggcggg ggttgttgag ccagacgtcg acgccctgga
      181 cgaggaagcg cgcgatccgg atgtcgtagt cctcgacgat gaacacgcga ccgcggagct
      241 tggggctgcg cgaccggccg aagatgtcct ggatcacgcc ctggcccggg cggtcggccg
      301 ggtgggcctt gcccgcgaag atgacctgca tcgggcgccg ttcgtcccac aggaggcgcg
      361 ccagccggtc gaggtcgctg aacagcagcg ccgcccgctt gtaggtggcg aaccggcggg
      421 cgaagccgat cgtgaggatc gagggatcga ggacgccctc cagctcctcg agcgccgagg
      481 gcgcttcgcc gtggcgcgcg aactggttgc gcaggcggcc gcgggcgaag atcgcgagct
      541 ccagcttctg gcgctggtga gcctcccaca tctcgtccgg cggcaccttg gcgatccgct
      601 cccagaaccg cttcgacgcg ggctggtcgt ccatcgcgtc catgtcggcg ttggtgaagc
      661 gctcgatggc gtcgcgcatc ggctgaccga gccaggtcgg cgtgtggacg ccgttggtga
      721 gcccgaggat ctcgtgcggg ctgatgccct gccaggtgct gttcgcggtg ttgccgtgga
      781 gcagcgagac cgcgttggcg ccgtgcgtcg tgcgcaggca cagggccgtc atgtcgaact
      841 gcgacgggtc gttctcgacg cccaggccca tcgtgagcag gcgctcgacc ggcacgccgc
      901 cggtgctggg gcgcccgtca ccgtcgagca gcgggccggc gacgcggcgc acgaggtccg
      961 cgtcgaagcg ctcgttgccg gccgagaccg gcgtgtggat cgtgaacacg ctgttgcggc
     1021 ggaccatcga gaacgcgtcg tcgagcgact tgcccgacgc gaccagctcg cgggcgcgct
     1081 cggcgagcag gaacgcggag tggccctcgt tgaggtgcca gaccgccggg tcgaggtcga
     1141 gctggcgcag cgcccgcacg ccgcctacgc ccagcaccag ctcctggtgc aggcgcatct
     1201 cgcgcccgcg gacgtacagg atgtgggtga tcggccggtc cgagtcgtcg ttgtcgggca
     1261 cgtcggtgtc gagcagcagg accggcaccc ggcccacctg gacgacccac accgcggcga
     1321 acagctcgcg accgggcagc gggacgctga ccatgagcgg ctcgccgagc gggtcgaggg
     1381 cccggcggat gggcaggcgg gcgggctcgt agtcggggta cgcgtgctcc tggtgcccgt
     1441 cggcgtcgat cgtctggcgg aagtagccct tgcggtacag cagccccaca ccgatcagcg
     1501 gcagcgccat gtcggaggcc gtcttcatgt ggtcgcccgc gaggacgccg aggccgcccg
     1561 agtagatccc gagtgactcg tggaggccgt attccgcgca gaagtaggcg atgggcccct
     1621 cgagctcctt ggcgtgccgg cgctggaacc agtggtccgc gccgtttgcc atgtacgcct
     1681 cgaactcggc gaggatgtcg tggtagtcgg ccatgaagtc ggtgttgtcg agcagcagcg
     1741 accagtcgac cggacccgac agcaccggaa tggggttgcg gtagcgggcc cacccaccgg
     1801 agtcgatgcg cgagaacatg atccgggccc gcgggtgcca gctccaccac atgttgtagg
     1861 cgagggtgcg gaggccttcc agcttgggcg gcaggtggaa ggaggcgccg ggcgcgaccg
     1921 gcacgtgcac caaaggctcc attggcggcc atcatagccg ggcgttcccg cccagagtga
     1981 accggtttag gaggtccggc ggcggctccg ggcggcgagt aggatcggtc gatgacgatg
     2041 cgggtggcga tgctcgccgc ggagtgcgag ccgtgggcga agaccggcgg cctcgccgac
     2101 gtcgtggacg cgctggcccg ggcgctgggc cggctgcctg aggcggggac cgagctggcg
     2161 gccccggtcg acgtcttcct gccgcgctac cggtccgtgc cggtgccgga cggggcgctg
     2221 accgagccca ccctgacgat ccctgatccg gatcgcgccc gtcgcggggc gcccatcgcc
     2281 gtctcgatcg tgaacgtggc ggccgatggc taccgcctgc gcctggtcga cgtccccgcg
     2341 gcgttcgacc gcgacgcgtt ctacgaccac cccgacgacc cgtggcggtt cgccgtgttc
     2401 tgccgcgcgg cgctggccgc gctgcgacgc gacgcacgac ccatcgacat cgtcaacgtc
     2461 cacgactggc acacgggccc ggcgctgctc gagcgcgccc gcgcggacgc cgcccgggac
     2521 gcgttcttcg cccggaccgc cgtcgtcgcg acgctccaca acctcgccta ccacggctgg
     2581 acgggcaacg accggatcgg ccagctcggc ttgcgggcgg gcgagccgct cgccggtgcc
     2641 catccggatg gcatcgacct gctgctgacg gcgatcgatc gatccgagat cgcaaacacc
     2701 gtgtcgcccg ggttcgccc
//
LOCUS       DBNHXO010000409         4476 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5081805, whole genome shotgun sequence.
ACCESSION   DBNHXO010000409 DBNHXO010000000
VERSION     DBNHXO010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5081805"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..122
                     /locus_tag="AC162A_08270"
     CDS             <1..122
                     /locus_tag="AC162A_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu C-terminal domain-related protein"
                     /protein_id="HIR5010875.1"
                     /translation="NVDMTVELITPIALEVGQRFAIREGGRTVGAGAITSITA"
     gene            133..297
                     /gene="rpmG"
                     /locus_tag="AC162A_08275"
     CDS             133..297
                     /gene="rpmG"
                     /locus_tag="AC162A_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR5010876.1"
                     /translation="MARVEARPVIQLACTECKTRNYTTRKNKKNDPNRIELLKYCPRE
                     RRHTLHREAK"
     gene            311..386
                     /locus_tag="AC162A_08280"
     tRNA            311..386
                     /locus_tag="AC162A_08280"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Trp,seq:cca)
     gene            414..608
                     /gene="secE"
                     /locus_tag="AC162A_08285"
     CDS             414..608
                     /gene="secE"
                     /locus_tag="AC162A_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR5010877.1"
                     /translation="MKRFRQFIKESWSELKKVSWPSRIQVRNLTILVFVISFVVGLYI
                     TIADTGFDYIVRWLSGLNAA"
     gene            605..1177
                     /gene="nusG"
                     /locus_tag="AC162A_08290"
     CDS             605..1177
                     /gene="nusG"
                     /locus_tag="AC162A_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR5010878.1"
                     /translation="MTEQLTATRAPEKRWYVIHTYSGYENKVKANLEHRIESMGMEDK
                     IFQVLVPMEDEIEIRQGQRHTVQKKVYPGYVLVEMILDPESWFVVRNTPGVTSFVGSG
                     NIPGMRSEPIPLDETEVKQILRHMGVETPKYRVAFTKGQSVRVTDGPFAEFIGTVDEV
                     NPERNKLKVLVSIFGRETPVELDFLQVEKL"
     gene            1198..1623
                     /gene="rplK"
                     /locus_tag="AC162A_08295"
     CDS             1198..1623
                     /gene="rplK"
                     /locus_tag="AC162A_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR5010879.1"
                     /translation="MAKKIRIVLTLQLPAGKATPAPPVGTALGPHGINIVEFTKSYNE
                     RTADKSGQVIPAQITIFEDRSFSFVLKTPPAPDLLRKAAGIDKGSETARRVMVGRVTR
                     DQVRQIAEIKMPDLNVNDVEAGMKVIEGTARSMGIEVVG"
     gene            1725..2432
                     /gene="rplA"
                     /locus_tag="AC162A_08300"
     CDS             1725..2432
                     /gene="rplA"
                     /locus_tag="AC162A_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR5010880.1"
                     /translation="MALHGKRYQDAAKLIDRDRIYPIDEAVELARQTSNVKFDAAVEA
                     HIRLGVDPRHADQMVRGTVVLPHGTGKVVRVAVFAQGEKAQEALRAGADEVGAEDLVK
                     KIEAGWLEFDVALAAPDVMGLVGRLGRILGRRGLMPNPKSGTITFDLDRAIREVKSGR
                     VEFKVDKGAIIHVSVGKASFEQAQLVENLATLVDAVNRAKPAGAKGQYLRTLTIATTM
                     GPGARVDIPSVLAAAQA"
     gene            2865..3395
                     /gene="rplJ"
                     /locus_tag="AC162A_08305"
     CDS             2865..3395
                     /gene="rplJ"
                     /locus_tag="AC162A_08305"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR5010881.1"
                     /translation="MPTEAKREMIDDLRAQLDASSTMIVSEYRGLKVKEISEIRRALR
                     KQNVSYRVVKNRLMRIAAQDSPAAAAISPLLVGPTAIAFGTDEAGTAKAVIDATKPYN
                     RIVKITGGVLGAQAIDADAVTRLAALPSRETLLAEAIGAITAPMATMAGLFDAPLRDI
                     AGLISAYHDQRAETAA"
     gene            3460..3855
                     /gene="rplL"
                     /locus_tag="AC162A_08310"
     CDS             3460..3855
                     /gene="rplL"
                     /locus_tag="AC162A_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012513304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR5010882.1"
                     /translation="MATMTPDQLLEAFEKMTVLELSEFKKKFEDRFGVTAAAPVAFAA
                     GAAPAAGGAPAAEASEEQTEFTATLTEIGPNKIPVIKVVRELTGLGLKEAKDLVDAAP
                     KAVKEGVAKDEAEKIKAALEEQGAKVEIK"
     gene            3929..>4476
                     /locus_tag="AC162A_08315"
     CDS             3929..>4476
                     /locus_tag="AC162A_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR5010883.1"
                     /translation="MTTPVLPPELADARVVDFPLRGQWVAVHSPADRIPSHGTDVLGQ
                     RYAFDFIRFDPRPGAAYRPPGGLRGLLFGVPTRECHGWGEPVHAPLDGEVVTAVDGMS
                     ERRRVHVVRELLLVLRNGLAFKPTTDWVRRLVGNHVILRHGDVYSVYAHLAPGSVAVD
                     AGEAVRTGDVLGRVGHTGNSTAP"
BASE COUNT          791 a         1576 c         1511 g          598 t
ORIGIN      
        1 acaacgtgga catgacggtc gagctgatca ccccgatcgc gctcgaggtc ggccagcgct
       61 tcgccatccg cgagggcggc cgcaccgtcg gcgccggcgc catcaccagc atcacggcct
      121 gacgagaccc ttatggccag agtcgaagcg cgcccggtga tccagctggc gtgcaccgag
      181 tgcaagacgc ggaactacac gacccgcaag aacaagaaga acgatcccaa ccggatcgag
      241 ctgctgaagt actgcccccg ggagcggcgc cacacgctgc accgggaggc gaagtagccg
      301 caccggacac aggggcgtag ctcaattggc agagcaccgg tctccaaaac cggttgttgt
      361 aggttcgagt cctatcgccc ctgccacctc ctccaaccgc tgaaaaggca tccgtgaaac
      421 gatttcgcca gttcatcaag gaatcgtggt ccgagctcaa gaaggtctcg tggcccagcc
      481 gcatccaggt ccggaacctc acgatcctcg tgttcgtgat ctcgttcgtg gtcgggctgt
      541 acatcacgat tgccgacacg ggcttcgact acatcgttcg ctggctgtcg gggttgaacg
      601 cagcatgacc gagcaactga ccgccacccg cgcccccgag aagcgctggt acgtgatcca
      661 cacctactcg ggctacgaga acaaggtcaa ggcgaacctc gagcaccgca tcgagtcgat
      721 gggcatggag gacaagatct tccaggtgct cgtcccgatg gaggacgaga tcgagatccg
      781 ccagggccag cggcacaccg tccagaagaa ggtctacccg ggctacgtgc tggtcgagat
      841 gatcctcgac cccgagtcct ggttcgtcgt ccgcaacacg ccgggcgtga ccagcttcgt
      901 cggcagcggc aacatccccg ggatgcgcag cgagccgatc ccgctggacg agaccgaggt
      961 caagcagatt ctgcggcaca tgggcgtcga gacacccaag taccgggtgg cgttcacgaa
     1021 gggccagagc gttcgcgtga ccgacggccc gttcgccgag ttcatcggca cggttgacga
     1081 ggtcaacccg gagcgcaaca agttgaaggt cctcgtgagc atcttcggcc gggagacgcc
     1141 ggtcgagctc gacttcctgc aagtcgaaaa gctctaggac agggagcgag caagaacgtg
     1201 gccaagaaga tccggatcgt cctcacgctg cagctgccgg ccggcaaggc gacacccgcc
     1261 ccgcctgtcg gcaccgcgct gggaccccac ggcatcaaca tcgtcgagtt caccaagtcg
     1321 tacaacgagc gcacggccga caagtcgggc caggtgatcc cggcccagat cacgatcttc
     1381 gaggaccggt cgttctcgtt cgtcctcaag acgccgccgg cgccggacct cctgcgcaag
     1441 gccgcgggga tcgacaaggg ctccgagacc gcacgacgcg tgatggtcgg ccgcgtgacc
     1501 cgcgaccagg tgcgccagat cgccgagatc aagatgcccg acctcaacgt caacgacgtc
     1561 gaggccggca tgaaggtcat cgagggcacg gcccgcagca tgggcatcga ggtcgtcggg
     1621 taggaatcgt tgccggcgaa ccgccggcga gcgcgtgcgg gaatgacccc ggaccggcgc
     1681 gccaccacca ggggcaggtc ttcggaccga agggagcaca cgacatggcc ttgcacggca
     1741 agcgctacca ggacgcggcg aagctcatcg accgcgaccg catctaccca atcgacgagg
     1801 ccgtcgagct cgcccgccag acctcgaacg tcaagttcga tgccgcggtg gaggcgcaca
     1861 tccgccttgg cgtcgacccc cgccacgccg accagatggt ccgcggcacg gtcgtcctgc
     1921 cgcacggcac cggcaaggtc gtccgggtgg ccgtgttcgc ccagggcgag aaggcccagg
     1981 aggcgctgcg cgccggcgcc gacgaggtcg gcgcggagga tctggtcaag aagatcgagg
     2041 ccggctggct cgagttcgac gtcgcgctcg ccgcgcccga cgtcatgggc ctggtgggcc
     2101 gcctcggccg catcctcggc cggcgcgggc tgatgccgaa cccgaagtcg ggcacgatca
     2161 cgttcgatct cgaccgcgcg atccgcgagg tcaagtcggg ccgcgtcgag ttcaaggtcg
     2221 acaagggcgc gatcatccac gtctcggtcg gcaaggcgag cttcgagcag gcccagctgg
     2281 tcgagaacct ggccaccctc gtcgacgcgg tgaaccgcgc gaagcccgcc ggcgcgaagg
     2341 gccagtacct gcgcacgctg acgatcgcga ccacgatggg tccgggagcc cgcgtcgaca
     2401 tcccgagcgt ccttgcggcc gcccaggctt agaggcgcta tcatcggccg gctctgatcg
     2461 ggcccgtctg ccgcgttggc cagtccgggc ggtcgctcac gcaccctgag gtgcgctcgc
     2521 gatccgtcct cccggccgcc tcgcatccgg acccgctgag agccgggaaa caattcaaga
     2581 gagacggatc accgcagaca gcctgcgctc gcggagcgcc gcacgaccga gaggtcgtcg
     2641 cgacgttcgg ggcttgatcc gccggaccgg accgccggcc acccgacgag ggtgacggca
     2701 ggggacgacg gcgagcacgc cgaggcagag gcatcgcccc acctccgagg tgggtcgcgc
     2761 cccctgcacg tggcccgtcg caggggttgt cagccggcat cgccggtgcg cacccaccgc
     2821 cgcgcgccgg ctgcgcgccg aagcatggag gtcaaggagg ctcgatgccg accgaggcca
     2881 agcgcgagat gatcgacgat ctgcgtgccc agctggacgc cagcagcacg atgatcgtgt
     2941 ccgagtatcg cggcctcaag gtgaaggaga tcagcgagat ccggcgcgcg ctgcgcaagc
     3001 agaacgtgag ctaccgggtc gtcaagaacc gcctgatgcg gatcgcggcg caggacagcc
     3061 ctgccgcggc cgcgatctcg cccctgctcg tgggaccgac ggcgatcgcc ttcggcaccg
     3121 acgaggcggg cacggcgaag gcggtcatcg acgccaccaa gccctacaac cgcatcgtga
     3181 agatcacggg cggggtgctg ggcgcccagg cgatcgatgc cgacgcggtg acccgcctgg
     3241 cggccctgcc gtcacgcgag acgctgctcg ccgaggccat tggcgcgatc accgccccga
     3301 tggccacgat ggccggactg ttcgacgcac cgctgcgcga catcgccggg ctcatctcgg
     3361 cgtaccacga ccagcgggcc gagacggccg cctgatcccc acctcgagac cgacccgcgt
     3421 cgcgttgccg cgacgccaac cccaggagaa caccacacca tggccacgat gacccccgac
     3481 cagctgctcg aggcgttcga gaagatgacc gttctcgagc tgtccgagtt caagaagaag
     3541 ttcgaggacc gcttcggcgt gaccgccgcc gcgcccgtcg ccttcgccgc cggcgccgcg
     3601 ccggccgccg gtggtgcgcc ggccgccgag gcctccgagg agcagaccga gttcaccgcg
     3661 accctgaccg agatcggccc caacaagatc ccggtcatca aggtcgtccg cgagctcacc
     3721 ggcctgggcc tcaaggaggc caaggacctc gtcgacgcgg cccccaaggc cgtcaaggaa
     3781 ggcgtcgcca aggacgaggc cgagaagatc aaggccgcgc tcgaggagca gggcgccaag
     3841 gtcgagatca agtaggtccg ttcgccagcg cgagcctggg agccggtcca gccaccgtgg
     3901 cgggccggct ctcgtgcatt ccgccgccgt gacgacaccg gtgctcccgc ccgagctcgc
     3961 cgacgcccgc gtggtcgact tcccgctgcg cgggcagtgg gtcgccgtgc actcgccggc
     4021 cgaccgcatc cccagccacg gcaccgacgt gctcggccag cgctacgcgt tcgacttcat
     4081 tcggttcgat ccgcggccgg gcgcggcata ccgtccgcct ggcggcctgc gcggcctgct
     4141 gttcggcgtg ccgacccgcg agtgccacgg gtggggcgag ccggtccacg cgccactcga
     4201 cggcgaggtc gtcacggccg tcgacgggat gtccgagcgc cggcgggtcc acgtcgtgcg
     4261 cgagctgctg ctggtgcttc gcaacggcct ggcgttcaag cccactacgg actgggtccg
     4321 ccgcctcgtc ggcaaccacg tgatcctgcg ccacggggac gtgtactcgg tctacgccca
     4381 cctggcgccg gggtcggtgg ccgtcgatgc gggcgaggcc gtgcgcaccg gtgacgtgct
     4441 cgggcgagtg gggcatacgg gcaactcgac cgcgcc
//
LOCUS       DBNHXO010000410         4573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1707172, whole genome shotgun sequence.
ACCESSION   DBNHXO010000410 DBNHXO010000000
VERSION     DBNHXO010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1707172"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..459)
                     /locus_tag="AC162A_08320"
     CDS             complement(73..459)
                     /locus_tag="AC162A_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010884.1"
                     /translation="MGGKTAIAAGLIMGVAVGAVSLGGIVYLAPNLPPIATPVPVTPA
                     PSASEAPSPSAVPSPSPAPSASAPGSASPATSSAAPSTSPEASGSAEPSGSPGPSTPA
                     EGALFGIDQDELPDVTVTPQRNEPAA"
     gene            529..1290
                     /locus_tag="AC162A_08325"
     CDS             529..1290
                     /locus_tag="AC162A_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010885.1"
                     /translation="MAPRPTSIVSIARTRLLPALLTALGVVLIVAGLLSYADPTTAGT
                     VLEESPTTVEQSDAPSVPASGDLPSPSDGSSAEPTATATPKPSGSGEPAHATRVVIPA
                     LKIDLPVVAGPNGYPFCNVAMYIDNLGKPLVDLGQPGRGIATYLFAHARDGMFGPIYE
                     NAIAKHQPAKMLGMIVQLYTSDDKLYLFEIRKVLLHQLSLDAAFAADTEQLWLQTSEG
                     PKGTPGKTQVKALLLSVGDADPRDAHPKAKPVNCQ"
     gene            complement(1301..2329)
                     /locus_tag="AC162A_08330"
     CDS             complement(1301..2329)
                     /locus_tag="AC162A_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIR5010886.1"
                     /translation="MQEAIPTSSSRSSAEPDPATLDPLVVRGCSWTNGVSRAFEGIDE
                     LKAVIADRESRVWVDVTARPEAPVDAITQLLDLHPLIASDIGERNHRAKVAEVEGAIH
                     VVMFWIAYAGAVTELEVDIVLDKRSLLTVHEPGFDPFALPQLRGDGSGLLKRGPDFLL
                     YAITDGIVDAYFPVLDSLEDEIDEIQDDVIARPTTWTLERLFAIKRELIGLRRAISPA
                     REIFNQLTNRDQGLIAPEHIVYFRDVYDHLIRVTDELDTDRELVAGTLEVYLSTVNNN
                     LSTIMKRLTGVTVILAGIGAVAGIFGMSEAGSALSNGEGAGFWAISGLVVLGAAMTAI
                     FLRRIDWI"
     gene            complement(2280..3977)
                     /locus_tag="AC162A_08335"
     CDS             complement(2280..3977)
                     /locus_tag="AC162A_08335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5010887.1"
                     /translation="MEQLSRRDRIVLLAVMGTGVFLAGLELMVTAVALPAIVADIAGW
                     TRLREASWIINGYLLVSVVTMPLAGRMADLWGTRRLFLGALAVFTLGSLLAGMAPTLE
                     LLIAARLVQAVGGGALIPVATSGASHLFPGAGRPRALGVVASLTFLGMAAGPFLGAAI
                     LGAVYPAAALSQLGVDPATLPGSLLVPAWRWVFYVNVPIGIIALVVAWAASSGWETPR
                     RPGRLDIVGGLLFSAFLVGGLGALTLLGSQAEPASDGPDPMVVAAVLAVVAIVTGVAS
                     VMRGLRVRDPFLDPRLFRHAGFASATAVSLLTGYGLATAIVGAAVFVDRVLYGGPDEQ
                     RLALGALAGATAVGALSSGFIVRVVSLRLVTLAGLTASIVALVGMSGWTTASPVGAAA
                     LALAVFGLGFGATVTPRSTAAVAAVGREAYGMAASTVTVARMVGMAIGLAILTAYGST
                     TIDRLYDQVYATADSYQAYIPPSLQDRPLRDGLVIEALENWAAGEAAEIMVGVFLVAA
                     VVTALAIPPSLLLDRRRMLGSTGPVQTPDDAAGAPSGATDADAGSDPDLELPVIGGA"
     gene            4203..>4573
                     /locus_tag="AC162A_08340"
     CDS             4203..>4573
                     /locus_tag="AC162A_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014036.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HIR5010888.1"
                     /translation="MPHRTFAQVAKRRLVGKEGRERIREVRALLDELPDYKNGPYADL
                     RKALMAEIEDTRVRSSAVHRDSIAVRREGAAQIALVGPPNVGKSSVLQALSEIQIRTG
                     DYAFTTLRPVPALTRIGGVLV"
BASE COUNT          735 a         1646 c         1637 g          555 t
ORIGIN      
        1 cctcgggctg ttcccgacca cgatgctcga gatcgtcggg cgggcggccg cggccgtcgg
       61 acaggtcggc ggctaggcgg ccggttcgtt tctctggggc gtgacggtca cgtccggcag
      121 ctcgtcctgg tcgatcccga acagcgcacc ctcggcgggg gtcgaagggc cgggcgatcc
      181 cgatggctcg gctgagcccg acgcctccgg cgaggtcgaa ggtgcggccg acgaggtcgc
      241 cggagatgcg gagcctggag ccgacgccga tggggcaggc gaaggcgagg gcacggccga
      301 aggcgacggg gcctccgagg ccgatggggc cggcgtgacc ggcaccggtg tcgcgatcgg
      361 cggcaggttg ggcgccaggt acacgattcc gcccagggac accgccccga cggcgacacc
      421 catgatcagg cccgccgcaa tcgccgtctt gccgcccatc ggcagcgagc gtaccagtac
      481 cacccggtcg aaggcggacc cggccgcgct cgactacgat ggcgcgccat ggcccctcgt
      541 cccaccagca tcgtctccat cgcccgcacg cggctcctgc ccgcgctgct cacggcgctg
      601 ggcgtcgtcc tcatcgtcgc ggggctgctc tcctacgccg acccgaccac cgcaggcacg
      661 gtgctcgagg agtcgccgac gacggtcgag cagtcggacg cccccagcgt ccctgcgtcc
      721 ggcgacctgc cgtcgccgtc ggacggatcg agtgccgagc cgacggcgac ggcgacgccc
      781 aagccgtcgg gatcgggcga accggcgcac gcgacgcgcg tcgtgatccc ggccctcaag
      841 atcgacctcc ccgtggtcgc gggacccaac ggctacccgt tctgcaacgt cgcgatgtac
      901 atcgacaacc tgggcaagcc gctcgtggac ctgggccaac cggggcgcgg catcgcgacc
      961 tacctgttcg cccacgctcg cgacggcatg ttcggcccga tctacgagaa cgcgatcgcc
     1021 aagcaccagc ccgccaagat gctgggcatg atcgttcagc tctacacgag cgacgacaag
     1081 ctctacctgt tcgagatccg caaggtgctg ctgcaccagc tcagcctcga cgcggcgttc
     1141 gccgccgaca cggagcagct ctggctccag acgtccgagg gcccgaaggg cacaccgggc
     1201 aagacacagg tcaaggcgct cctgctttcg gtcggggacg cggaccccag ggacgcgcac
     1261 cccaaggcga agccggtcaa ctgccagtag gcgcggtcgg ctagatccag tcgatgcgcc
     1321 gcaggaagat cgcggtcatg gccgctccga gcaccacgag gcccgagatc gcccagaacc
     1381 cagcgccctc cccgttcgac agggccgacc cggcctcgct catcccgaag atgccggcga
     1441 ccgcgccgat gccggccagg atgaccgtga cgcctgtcag tcgcttcatg atggtcgaga
     1501 ggttgttgtt gaccgtggac aggtagacct cgagcgtgcc cgccaccagc tcccggtccg
     1561 tgtcgagctc gtccgtgacg cggatcaggt ggtcgtagac gtcgcggaag tagacgatgt
     1621 gctcgggagc gatcagcccc tggtcccggt tggtcagctg gttgaagatc tcgcgcgccg
     1681 gcgagatcgc gcgccgcagg ccgatgagct cgcgcttgat cgcgaacagg cgctcgagcg
     1741 tccacgtggt cggccgagcg atgacatcgt cctggatctc atcgatctcg tcctcgagcg
     1801 agtcgaggac cgggaagtag gcgtcgacga tgccgtcggt gatcgcatac agcaggaagt
     1861 ccggcccgcg cttcaggagc cccgagccgt cgccgcgcag ctgcggcagc gcgaacgggt
     1921 cgaaaccggg ctcgtggacg gtcagcagcg accgcttgtc cagcacgatg tcgacctcga
     1981 gctcagtcac cgcgccggca tacgcgatcc agaacatcac gacgtggatc gccccttcga
     2041 cctcggcgac cttggcgcga tggttgcgct cgccgatgtc cgacgcgatc agcgggtgga
     2101 ggtcgagcag ctgggtgatc gcgtccaccg gggcctccgg tcgggccgtg acgtcgaccc
     2161 agacacgcga ctccctgtcc gcgattacgg ccttcagctc gtcgatgccc tcgaaggcgc
     2221 ggctgacacc gttcgtccac gaacagccac gcaccaccag cgggtcgagc gtggccggat
     2281 caggctccgc cgatgaccgg gagctcgagg tcgggatcgc ttcctgcatc ggcgtcggtg
     2341 gctccgctcg gggcgcctgc ggcatcgtcc ggcgtctgga cgggcccggt cgagccgagc
     2401 atacgccgac gatcgaggag cagggacggc gggatcgcga gcgccgtcac cacggcggcc
     2461 acgaggaaca cgccgaccat gatctcggcc gcctcgcccg ccgcccagtt ctcgagggcc
     2521 tcgatgacga ggccgtcgcg caggggccgg tcctggaggg acggcgggat gtaggcctgg
     2581 tacgagtcgg cggtcgcgta cacctggtcg tagaggcggt cgatggtcgt cgagccgtag
     2641 gccgtgagaa tcgcgagccc gatggccatg cccaccatcc gcgccacggt cacggtggac
     2701 gcggccatcc cgtaggcctc gcggcccacc gccgcgacgg cggccgtcga gcggggcgtg
     2761 acggtggccc cgaagccgag gccgaacacc gcgagcgcga gcgccgcggc gccgacgggg
     2821 gaggccgtcg tccagcccga catcccgacc agcgcgacga tcgacgcggt gaggccggcc
     2881 agcgtcacca gccgcagcga cacgacccgc acgatgaacc cggacgacag ggcgccgacg
     2941 gccgtcgccc ccgcgagggc gccgagcgcg aggcgctgct cgtcggggcc gccgtacagc
     3001 acccggtcga cgaacaccgc ggcgccgacg atcgcggtgg ccaggccgta cccggtcagc
     3061 agggacacgg ccgtcgccga ggcgaagccc gcgtgccgga acagccgcgg gtcgaggaac
     3121 gggtcgcgga cccgcagccc gcgcatgacc gaggccacgc ccgtgacgat cgcgacgacg
     3181 gccagcaccg ccgcgacgac catcgggtcc ggtccgtccg acgccggttc agcctggctt
     3241 ccgagcagcg tcagcgcccc gagcccgccg accaggaacg cggagaacag gaggccgccg
     3301 acgatgtcga ggcggcccgg ccggcgcggc gtctcccagc cgctgctggc cgcccacgcg
     3361 acaacgaggg cgatgatccc gatcggcacg ttgacgtaga acacccagcg ccacgcgggc
     3421 acgagcaggc tgccgggaag cgtcgcggga tcgacgccca gctgcgacaa ggccgccgcc
     3481 ggatagacgg cacccaggat cgccgcgccg aggaacggtc cggcggccat gcccaggaac
     3541 gtcagcgacg cgacgacgcc cagggcgcgc ggacgtccgg cgcccgggaa caggtgcgac
     3601 gccccggaag tggccacggg gatgagcgcg ccgccaccca ccgcctggac gagccgtgcc
     3661 gcgatcagca gctcgagcgt cggcgccatg ccggccagca ggctgcccag cgtgaacacg
     3721 gcgagcgcgc cgaggaacag tcggcgcgtg ccccacaggt cggccatgcg cccggccagc
     3781 ggcatcgtga ccacggacac gagcaggtac ccgttgatga tccacgaggc ctcgcgcagg
     3841 cgcgtccagc cggcgatgtc ggcgacgatc gcgggcaggg cgacggccgt gaccatgagc
     3901 tcgaggcccg ccaggaacac gccggtgccc atgacggcga gcaggacgat gcggtcgcgg
     3961 cgcgagagct gttccacggg cgcgagtgta ggggtggacc acggatcggg tcgcggtgta
     4021 ggctgccggt ccgatgaacc ccgcctggta cgccaccatc tcctgtcaga caggctgatc
     4081 ggccccttcc tcacccagcg tcggaggaag gggcgggtcg agacgcgagc cacaacagcc
     4141 ggtgcccgag cgcccggcgc ggcctcgcgt tccccgcaca gcggaaggag atggcgatgc
     4201 ccatgccgca tcgcacgttc gcacaggtgg cgaagcgcag gctggtcggc aaggaggggc
     4261 gggagcggat ccgcgaggtc cgcgccctgc tcgacgagct cccggactac aagaacgggc
     4321 cgtacgcgga cctgcgcaag gcgctcatgg ccgagatcga ggacacgcgc gtccggtcgt
     4381 cggcggtcca ccgcgactcg atcgccgtcc gccgcgaggg tgcggcgcag atcgcgctcg
     4441 tcgggccgcc caacgtcggc aagtcgtccg tgctccaggc gctgtcggag atccagatcc
     4501 gcacgggcga ctacgcgttc acgaccctcc ggccggtgcc cgcgctcacg cgcatcggcg
     4561 gggtgctggt cca
//
LOCUS       DBNHXO010000411         2703 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5479379, whole genome shotgun sequence.
ACCESSION   DBNHXO010000411 DBNHXO010000000
VERSION     DBNHXO010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5479379"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="AC162A_08345"
     CDS             complement(<1..504)
                     /locus_tag="AC162A_08345"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR5010889.1"
                     /translation="MFADLTPLAVVLAILGGGWGFVADRISARWPEHEDGSIRPIDWR
                     TPVVVVIGAIALGALTLRFPDPVPAVIFGAYSLALVLMLATDLDQRLLPNELTLPAIP
                     IALVVGLAGLNPLVPPADMPFAIAFALLVPGIIFLASIPFGAGAIGQGDLKLLISVGL
                     LAGAYRLF"
     gene            complement(553..2064)
                     /locus_tag="AC162A_08350"
     CDS             complement(553..2064)
                     /locus_tag="AC162A_08350"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013079.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HIR5010890.1"
                     /translation="MQIRVVTDQPWEVTTDVLVVPVVGEVEFSGAYDELDRRSGGELR
                     SLQAFGELRDKRYGTALAAAGELPARRVLVVSAGPGEDADRETIRKVASTAIRRLVNR
                     DVRTMAIWIDPLATALGGDASLAADLAVRGVVEGSFEPKTIYREDSDAAPPALDELVL
                     IASGGNKAALEKASERGRIIGEGANVARRLSNRAANDVSPQVLADEASEIAAANGLAI
                     DVLDEKRAAELGMGMFLAVGQGSANPPRMIVMRSGAEGEKDALGRQLALVGKGVCFDS
                     GGISIKPADRMDEMKMDKIGACTVIAAIATVARLSPGLPLLAVAPAVENMPGGGAARP
                     GDIIRAMNGKWVDLINTDAEGRLILGDAMTYAEQLGATHIVDVATLTGAVGRAIGNIV
                     TGAFATPDPWWTDLAAAADRAGERYWRIPLIADYMSEMESWYGDFQNTGTPDGSLVKS
                     GLFLEQFRTVPWAHLDIGGTGYFRKTYPFAPRGATGVTHATLVELALAGAAST"
     gene            complement(2182..>2703)
                     /locus_tag="AC162A_08355"
     CDS             complement(2182..>2703)
                     /locus_tag="AC162A_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010891.1"
                     /translation="DGLESDWAAFTGRALNAATLTSLLTRHDQRTASAASEGSAAHYR
                     QALDALDRSDSMIAEARELRDRLAATTDVETLSSWIDRNANYDAALRQLYEALLESKG
                     KVTDRVRSAIGAEQVARQQLPPDTRALVVIMSDIAQGGLNQAVIAIEEARGALGSALD
                     LQHRLQQSPELPG"
BASE COUNT          387 a          979 c          967 g          370 t
ORIGIN      
        1 gaacagccgg taggcgccgg cgagcaggcc caccgagatc agcagcttga ggtcgccctg
       61 gccgatcgcc ccggccccga acgggatcga ggccaggaag atgatcccgg gaaccagcag
      121 cgcgaacgcg atcgcgaacg gcatgtccgc cgggggcacg agcggattga gcccggcaag
      181 cccgacgacg agggcgatcg ggatcgcggg cagcgtgagc tcgttgggca gcagccgctg
      241 gtcgaggtca gtcgcgagca tcagcacgag ggcaagcgag tacgccccga agatcacggc
      301 cggcaccgga tccgggaagc gcagcgtgag ggcgcccagc gcgatggcgc cgatgaccac
      361 gacgacgggc gtccgccagt cgatcggccg gatgctgccg tcctcgtgct cgggccagcg
      421 ggccgagatc cggtcggcca cgaaccccca gccgccaccg aggatggcga ggacgacggc
      481 gagcggggtc aggtcggcga acacgccgcg gagtctacga ggggcgggcg accccgtgcg
      541 gtgacccgat ggtcaggtgg acgcggcccc cgccagcgcg agctccacga gcgtggcgtg
      601 cgtgaccccg gtcgcacctc gcggggcgaa cgggtacgtc ttgcggaagt agccggtccc
      661 gccgatgtcg aggtgcgccc acggcaccgt ccggaactgc tccaggaaca ggccgctctt
      721 gaccagcgag ccgtcgggcg tgccggtgtt ctggaagtcg ccgtaccagc tctccatctc
      781 ggacatgtag tcggcgatga gcgggatccg ccagtagcgc tcgccggccc ggtcggccgc
      841 cgcggcgagg tcggtccacc acgggtcggg cgtcgcgaag gcgccggtga cgatgttgcc
      901 gatcgcgcgg cccacggcgc ccgtcagcgt cgcgacgtcg acgatgtgcg tcgcgccaag
      961 ctgctccgcg taggtcatcg cgtcgcccag gatcagccgg ccctcggcat cggtattgat
     1021 gaggtcgacc cacttgccgt tcatcgcccg gatgatgtcg ccgggtcggg cggccccgcc
     1081 gccgggcatg ttctcgaccg caggggcgac cgcgagcagc ggcagccccg gcgacaggcg
     1141 ggcgacggtg gcgatggccg cgatcacggt gcaggcaccg atcttgtcca tcttcatctc
     1201 gtccatccgg tcggccggct tgatgctgat gccgcccgag tcgaagcaca cgcccttgcc
     1261 gaccagcgcc agctgccgcc ccagcgcgtc cttctcgccc tcggcgccgg agcgcatgac
     1321 gatcatccgc ggcgggttcg cgctcccctg cccgaccgcg aggaacatcc ccatcccgag
     1381 ctcggccgcg cgcttctcgt cgagcacgtc gatcgccagc ccgttggccg ccgcgatctc
     1441 gctcgcctcg tcggcgagga cctgcggcga gacgtcgttg gcggcccggt tcgacagccg
     1501 gcgggcgacg ttggccccct cgccgatgat ccggccacgt tcggaggcct tctccagcgc
     1561 cgccttgttc ccgccgctcg cgatcaggac cagctcgtcg agcgccgggg gcgccgcgtc
     1621 gctgtcctcg cggtagatcg tcttgggttc gaagctgcct tcgaccaccc cgcgcaccgc
     1681 gagatcggcg gccagcgacg catccccgcc gagcgccgtg gccagcgggt cgatccagat
     1741 cgccatcgtc ctgacgtcgc ggttgacgag gcgacggatc gcggtcgacg cgaccttgcg
     1801 gatggtctcg cggtcggcgt cctcgcctgg accggccgag acgaccagca cgcgacgcgc
     1861 cggcagctcg cctgcggccg cgagcgccgt cccgtaccgc ttgtcccgca gctcgccgaa
     1921 cgcctggagc gagcgcagct cgccgcccga gcgtcgatcg agctcgtcgt aggccccgct
     1981 gaactcgacc tcaccgacga ccggcacgac caatacgtcg gtcgtgacct cccacggctg
     2041 gtcggtgaca acgcgaatct gcacgaacag aatcctccgg tgggcatcac ggccagcagc
     2101 cccccatcgg ccggacgaag tgggagtttg gccgacagtg tagcggcggc catggcggcc
     2161 accgcaagga cgcccgaagg cctacccggg cagctcgggc gactgctgca accggtgctg
     2221 gaggtcgagc gcggagccca gggcgccacg cgcctcctcg atcgcgatca cggcctggtt
     2281 gagcccgccc tgcgcgatgt cggacatgat caccaccagc gcgcgcgtgt ccggcggcag
     2341 ctgctggcgg gcgacctgct cggcgccgat cgcggaccgc acccggtcgg tgaccttgcc
     2401 cttcgactcg aggagcgcct cgtacagctg gcgcagggcg gcgtcgtagt tggcgttccg
     2461 gtcgatccag ctcgatagcg tctccacgtc ggtcgtggcg gcgaggcgat cgcgaagctc
     2521 ccgggcctcg gcgatcatcg agtcggaccg gtccagcgcg tccagggcct gccgatagtg
     2581 cgcggcggat ccctccgagg ccgccgacgc ggtccgctgg tcgtgtcgcg tgaggaggct
     2641 ggtgagggtc gccgcgttca gggcgcgacc ggtgaacgcc gcccagtcgc tctcgagccc
     2701 gtc
//
LOCUS       DBNHXO010000412         5006 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2104888, whole genome shotgun sequence.
ACCESSION   DBNHXO010000412 DBNHXO010000000
VERSION     DBNHXO010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2104888"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1778
                     /locus_tag="AC162A_08360"
     CDS             <1..1778
                     /locus_tag="AC162A_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HIR5010892.1"
                     /translation="DGQVSRGGHEARARRDLLLPALQAAAARTGWVSRGALNHVSKRL
                     AVPPAEAYGVASFYSLLPTTPRPPIVAHVCDDIACRLLGAERICDELETRLGPPGETA
                     GHEDATWLRSPCLGQCERAPAVLVTSAGPTPKDHVRGGIRGADDVASMLTTVRLSPAL
                     TTDVGDAGELAPQKGDPSLRLLTRVGRVDPTSLSAYREADGYRALHKALSMGPAAVIE
                     QVTASKLVGRGGAAFPTGRKWAAVAAQPVTPHYVVCNADESEPGTFKDRVILEHDPFA
                     LLEAMTIAGFAVGASRGYLYLRGEYPLARRRLESALDQVRRADLLGPNVAGSGWSYDI
                     ELRIGAGAYIAGEETALFESIEGGRPEPRNKPPFPVEVGLFGKPTVVNNVETLANVPL
                     ILHDGGATYATIGTEGSTGPKLFCVSGNVERPGVYETDFGSTLGDIIDLAGGVAGRRR
                     IQAILLGGAAGAFVGPDTLGTRMTFEGTRAAGATLGSGVVMVFDETADLPDALRRIAA
                     FFRDESCGQCVPCRVGTVRQEELLARLAAGRPLGSLETELALFDDLARAMRDASICGL
                     GQTASSAIESAIKGGLVHFDAGAWA"
     gene            1775..2815
                     /locus_tag="AC162A_08365"
     CDS             1775..2815
                     /locus_tag="AC162A_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017555592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR5010893.1"
                     /translation="MTTEDIGHIFVDPPARASRIPGPPPAREVPLVEITIDGAAVSVP
                     AGSTILEAARSVGVDTPTLCYLENLTPVNVCRVCVVEVTGSRVLVPACSRRVEPGMEV
                     QTDSERVRHSRRLVLELLDSSVDVSLAGPAEPDGDLARYATQYGADGSRFGRSAAAAS
                     AGERDAREPGHHHAPVVDGRHAATAETVAQPVKVDNDLYVRDYSRCILCYKCVEACGE
                     DAQNTFAIAVAGRGFDARISTEFDVPLPESACVYCGNCIGVCPTGALMFRSEYEMRQA
                     GTWAPEEQTVTDTICPYCGVGCTLSLHVQDNRIVKVSSPSDSSVTDGHLCVKGRFGYD
                     FVQLLPKGSPRP"
     gene            complement(3005..3859)
                     /gene="fdhD"
                     /locus_tag="AC162A_08370"
     CDS             complement(3005..3859)
                     /gene="fdhD"
                     /locus_tag="AC162A_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HIR5010894.1"
                     /translation="MSSPTDPTPREASHARVIAVRGGRVEVREDRLSGEEPLAIRACG
                     PGEEPVDVAVTMRTPGHEDELAAGFLRTEGLIDDDTVITGITRGDPETHAQPDDEVIV
                     HLAGPFDASRVAERHFVATASCGICGKASLDEVEVRCDPIPAGPVIAPETLIALPVRL
                     REAQAVFEATGGLHTAGLFDPGGELVVLREDVGRHNALDKLVGSQFLAGALPLNDRIV
                     LVSGRTSFELVQKAAVAGVPILAAVSAPSDLAVEAAERLGVTLVGFLRGDGFNVYTHP
                     GRVALAQT"
     gene            complement(3898..4692)
                     /locus_tag="AC162A_08375"
     CDS             complement(3898..4692)
                     /locus_tag="AC162A_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010895.1"
                     /translation="MRLTLLSQSVLGVLLVAGCGLDGGPSPTPVSVPSAPSSAPTPSA
                     APTPEPSTAVDAGGPIDPAHFVATIDNPWYPLLPGATFHYRGTTGGEPTTDTYEVTTE
                     TKVVAGVTTTVIRDTVRVQGVVSERTEDWFAQDVDGNVWYFGEATAELNRKGKVLNTH
                     GSWEAGVDGAEPGIFMPAEPAVGLSFAQEHFPGEAEDWFVVMFTGTRVTVPAGTYRDA
                     LVTGEWTPLEPGVVGEKTYVQGVGQVMERDISGGDETLRLVRVTGP"
     gene            4794..>5006
                     /locus_tag="AC162A_08380"
     CDS             4794..>5006
                     /locus_tag="AC162A_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010896.1"
                     /translation="MADSSDRLGGEARALVVAARRATLATIAPDGRPRLVPVCFVLVD
                     EHVWIAIDDKPKRSADPMDLARVRDVL"
BASE COUNT          681 a         1835 c         1775 g          715 t
ORIGIN      
        1 ccgacggcca ggtctcacgg ggcggccacg aggcacgggc gcgccgggac ctcctgctcc
       61 cggccctcca ggcggccgcg gcgcggaccg gctgggtcag tcggggcgcc ctcaaccacg
      121 tctcgaaacg ccttgccgtg ccgcccgccg aggcctacgg cgtcgcttcg ttctattcgc
      181 tgctgccgac gacgccgcgc cccccgatcg tcgcccacgt gtgcgacgac atcgcctgcc
      241 gcctgctggg cgcggaacgg atctgcgatg agctggagac ccggctcgga ccgccgggtg
      301 agacggcggg ccacgaggac gcgacctggc tgcggagccc gtgcctcggg cagtgcgagc
      361 gggcacccgc agtgctggtg acgtcggcgg ggccgacgcc caaggaccat gtccggggcg
      421 gcatccgcgg cgccgacgac gtcgcgagca tgctcacgac cgtgcgcctc tccccggccc
      481 tcacgactga cgtcggtgac gcaggcgagc tggcgcccca gaagggcgac ccatccctgc
      541 ggctgctcac ccgggtggga cgtgtcgacc cgacgtcgct ctccgcctat cgcgaggccg
      601 acggctatcg ggcgttgcac aaggcgctgt cgatggggcc ggccgccgtc atcgagcagg
      661 tcacggcgtc gaagctcgtg ggccggggtg gcgcggcctt cccgaccggg cgcaagtggg
      721 ccgctgttgc cgcgcaaccg gttacgccgc actacgtcgt gtgcaacgcg gacgagtcgg
      781 agcccgggac gttcaaggac cgggtgatcc tcgaacacga cccgtttgcg ctgctcgagg
      841 cgatgacgat cgccgggttc gcggtcggcg cctcgcgcgg gtacctgtac ctgcggggtg
      901 agtacccgct cgcacgccgg cgtctcgagt ccgccctcga ccaggtccgg cgggccgacc
      961 tcctgggccc gaacgtcgcg ggctccggct ggtcgtacga catcgagctg cggatcggcg
     1021 ccggcgccta catcgcgggc gaggagaccg cgctgttcga gtcgatcgaa ggcggacgcc
     1081 cggagccgcg caacaagccg ccgtttcccg tagaggtcgg gttgttcggg aagccgacgg
     1141 tcgtcaacaa cgtggagacg cttgccaacg tgcccctgat cctccacgac ggcggcgcga
     1201 cgtacgcgac cattggcacg gagggctcca ccggcccgaa gctgttctgc gtgtccggca
     1261 acgtcgagcg ccccggcgta tacgagacgg acttcggatc gaccctcggc gacatcatcg
     1321 atctcgccgg cggcgtggcc ggccggcggc gcatccaggc gatcctgctc ggcggcgcgg
     1381 cgggcgcgtt cgtcgggccg gacacgctgg ggacgcggat gacgttcgag ggcacgcgcg
     1441 ccgccggggc gacgctcggg tcgggcgtcg tgatggtgtt cgacgagacc gcggacctgc
     1501 ccgacgccct gcgccggatc gccgccttct tccgcgacga gtcgtgcggg cagtgcgtcc
     1561 cgtgccgggt cggcaccgtg cgccaggagg agctgctggc ccggctcgcg gcgggtcgcc
     1621 cgctgggctc gctggaaacg gagctcgcgc tgttcgacga cctcgcccgg gccatgcgcg
     1681 acgcctcgat ctgcgggctc ggtcagacgg cgtcgtcagc gatcgaaagc gcgatcaagg
     1741 gcggcctcgt gcacttcgat gcgggggcgt gggcatgacg accgaggaca tcggccacat
     1801 cttcgtcgac ccacccgcac gggcgtcgcg cattccgggt ccgccgccgg cgcgcgaggt
     1861 ccccctggtc gagatcacga tcgatggcgc ggcagtgtcg gtcccggccg gctccacgat
     1921 cctcgaggcc gcgcgttcgg tgggcgtcga cacgccgacc ctgtgctacc tcgagaacct
     1981 caccccggtg aatgtgtgcc gcgtctgcgt cgtcgaggtc accgggtcgc gcgtcctcgt
     2041 ccccgcctgc tcgcgccgcg tcgagcccgg gatggaggtt cagacagaca gcgagcgtgt
     2101 ccgccactcg cgccgcctcg tgctggagct gcttgactcg tccgtcgacg tctccctcgc
     2161 cggcccggcc gaacccgacg gcgacctcgc gaggtacgcc acgcagtacg gcgccgacgg
     2221 ctcccggttc gggcggtcgg cagcggcggc ttcggcgggg gaacgcgatg cccgggagcc
     2281 cggccaccac catgcccccg tcgtcgacgg ccggcacgcc gcgaccgccg agaccgtcgc
     2341 ccagccggtc aaggtcgaca acgacctcta tgtccgcgac tactcgcgct gcatcctctg
     2401 ctacaagtgc gtcgaggcgt gcggcgagga cgcccagaac acgttcgcga tcgcggtcgc
     2461 cgggcgcggc ttcgatgccc ggatctcgac cgagttcgac gtccccctgc ccgagtccgc
     2521 gtgcgtctac tgcggcaact gcatcggcgt gtgcccgact ggcgcgctca tgttccgctc
     2581 cgagtacgag atgcgccagg ccggcacctg ggcgcccgag gaacagaccg tgaccgacac
     2641 gatctgcccg tactgcggcg tcggctgcac cctctcgctg cacgtccagg acaaccggat
     2701 cgtgaaggtc agctcgccat cggactcctc ggtcactgac ggccacctgt gcgtgaaggg
     2761 ccgcttcggc tacgacttcg tccagctgct accgaagggc tcgccgcgcc cctgacctgg
     2821 cctccccggg cctgatgctc cgcccgaggt ccagataccg cctgaggcgg tacgcgtcag
     2881 gcgtcgacgg cgggtccacg ctcaaggcac ttcccatcta ccaccgggag cggtggatgg
     2941 acccagatcg gtgccacgta ccgcccgggg cggtaaacga acccacggtc gggagccgcg
     3001 accgtcaggt ttgggccagc gccactcgcc ccgggtgcgt gtagacgttg aagccatcgc
     3061 cgcgcaggaa accgaccagc gtcacgccga gtcgctcggc cgcctcgacc gcgaggtccg
     3121 acggggccga gaccgccgcc aggatcggga cgcccgcgac cgccgccttc tggacgagtt
     3181 cgaagctcgt ccggccggac acgaggacga tccgatcgtt gagcggcagc gcccccgcga
     3241 ggaactggct ccccaccagc ttgtcgaggg cgttgtggcg gccgacgtcc tcgcgcaaca
     3301 cgacgagctc gccgcccgga tcgaacagcc ccgccgtgtg caacccgccc gtcgcctcga
     3361 acacggcctg ggcctcgcgc agccgcacgg gcagcgcgat cagcgtctcg ggcgcgatga
     3421 ccgggccggc cgggatcggg tcgcagcgca cctcgacctc gtcgagcgac gccttgccgc
     3481 agatcccgca cgacgcggtg gccacgaagt gccgctccgc cacccgcgac gcgtcgaacg
     3541 ggccggccag gtggacgatg acctcgtcgt cgggctgggc gtgcgtctcc ggatcgccgc
     3601 gggtgatccc ggtgatcacg gtgtcgtcgt cgatcagccc ttccgtccgc agaaatccag
     3661 ccgccagctc gtcctcgtga ccgggcgtgc gcatcgtgac cgcgacgtcg accggctcct
     3721 caccgggacc gcaggcccgg atcgcgagcg gctcctcgcc cgacagccgg tcctcgcgca
     3781 cctcgacccg cccgccacgg acggcgatca cgcgggcgtg cgaggcctcg cggggcgtcg
     3841 gatcggtcgg gctgctcacg cgccggaatc taccccggcc ggcgcgggcc gccgtcgtca
     3901 ggggccggtg acgcgaacga gccgcagcgt ctcgtcgccg ccggagatgt cgcgctccat
     3961 cacctggccg acgccctgga cgtaggtctt ctcgccgacg acccctggct cgaggggtgt
     4021 ccactcgccg gtgaccagtg cgtccctgta cgtcccggcg ggcacggtca cgcgggtgcc
     4081 cgtgaacatc accacgaacc agtcctccgc ctcgccaggg aaatgctcct gggcgaacga
     4141 caggccgacg gccggctcgg cgggcatgaa gatgcccggt tccgcgccgt cgacgccggc
     4201 ctcccacgat ccgtgggtgt tcagcacctt gcccttgcgg ttgagctcgg ctgtcgcctc
     4261 gccgaagtac cagacgttgc cgtcgacgtc ctgcgcgaac cagtcctcgg tgcgctcgga
     4321 cacgacgccc tgcacgcgga cggtgtcccg gatgacggtc gtggtgactc ccgcgacgac
     4381 cttggtctcg gtggtgacct cgtaggtgtc ggtggttggc tcgccgccgg tcgtcccgcg
     4441 gtaatggaac gtcgcgccgg gcaggagcgg ataccagggg ttgtcgatgg tggcgacgaa
     4501 gtgcgccggg tcgatgggcc cgccggcatc gacggccgtt gacggctcag gcgtgggggc
     4561 ggccgagggc gtcggggcgc tcgatggggc cgaggggacc gacacgggag tcggcgacgg
     4621 gccaccgtcg aggccgcagc cggcgaccag caggacgcca aggacggatt ggctcagcag
     4681 ggtcaggcgc atggcacacg ctccgcacgc ggttccaaca ctcgttcacc agatcgaggc
     4741 tgttcggcgc ccgaaggttt caccggtggt atggccacgg cgatactgcc gccatggccg
     4801 acagttccga ccgtctcggg ggcgaggcac gcgccttggt tgtcgccgcg cgccgcgcca
     4861 ccctcgcgac gatcgcgccc gacggccggc ctcggctcgt gccggtctgc ttcgtgctcg
     4921 tcgacgagca cgtctggatc gccatcgacg acaaacccaa gcgctcggcg gaccctatgg
     4981 acctggctcg cgtccgagac gtgctc
//
LOCUS       DBNHXO010000413         1781 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2105254, whole genome shotgun sequence.
ACCESSION   DBNHXO010000413 DBNHXO010000000
VERSION     DBNHXO010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2105254"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="AC162A_08385"
     CDS             complement(<1..276)
                     /locus_tag="AC162A_08385"
                     /inference="COORDINATES: protein motif:HMM:NF025973.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HIR5010897.1"
                     /translation="MHPADALTERLGLPVRDRDLLAQALIHSSWLHEHPDAAQGHNER
                     LEFLGDAVVSLAVSEALYKRHPADDEGVLSARRAAIVSTPGLARLAAR"
     gene            complement(297..533)
                     /gene="acpP"
                     /locus_tag="AC162A_08390"
     CDS             complement(297..533)
                     /gene="acpP"
                     /locus_tag="AC162A_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR5010898.1"
                     /translation="MASTYDRLKKIVIEQLGVDEAEVNPEASFVDDLNADSLDLVELI
                     MSLEEEFGTEISDEDAEKIRTVQDAVDYIDEHSS"
     gene            complement(563..1030)
                     /gene="nusB"
                     /locus_tag="AC162A_08395"
     CDS             complement(563..1030)
                     /gene="nusB"
                     /locus_tag="AC162A_08395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR5010899.1"
                     /translation="MASEPDSPRGRRDPAGDAHVRSRRAGRRLALASVFEAEFGQRTA
                     DAILERHLAMEGDADTAALARELVANVVAYRDSLDATIADAAPQYPVVQLARMDRALL
                     RCGLCEVLHSPATPARVAIAEWVELARTYSGEPMRRLMNGVLGRVARDMDAAR"
     gene            complement(1014..1325)
                     /locus_tag="AC162A_08400"
     CDS             complement(1014..1325)
                     /locus_tag="AC162A_08400"
                     /inference="COORDINATES: protein motif:HMM:NF015721.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR5010900.1"
                     /translation="MTPSLSVGPGVIRETIRLAALEVPGVLRVGRGGPRWRDVLGGSP
                     IRIRIRDGRVDVTVRLVARPRQPLVPLTAQVREAVGAAVERLLGMELGAVTVLVDGVG
                     A"
     gene            complement(1322..>1781)
                     /locus_tag="AC162A_08405"
     CDS             complement(1322..>1781)
                     /locus_tag="AC162A_08405"
                     /inference="COORDINATES: protein motif:HMM:NF014554.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR5010901.1"
                     /translation="LLDETDLNFVGNVEGKDLTRQMADVVVCDAVLGNVVIKFFEGLS
                     GFIFDLFRAEFTGSIRGRLAYVLMRPSIRRIRGVFDYEVVGGSPLLGVRGAVIITHGR
                     ARRRMIEHAVRVGAATARERVPERIGVALGEADDEPGVAAAPAIISEALT"
BASE COUNT          258 a          643 c          631 g          249 t
ORIGIN      
        1 gcgggccgcc agcctcgcga ggccgggcgt gctgacgatc gcggcgcgac gcgccgacag
       61 gacaccctcg tcgtccgccg gatggcgctt gtacagggcc tcggacacgg cgaggctcac
      121 aacggcatca cccaggaact cgaggcgctc gttgtggccc tgcgcggcgt cgggatgctc
      181 gtgcagccag ctgctgtgga tcagcgcctg ggccagcagg tcgcgatcgc ggacgggaag
      241 cccgaggcgc tccgtcaggg cgtcagccgg gtgcatcgca cccccgggtc gagccgctaa
      301 gacgagtgct cgtcgatgta gtcgacggcg tcctggacgg tccggatctt ctcggcatcc
      361 tcgtcggaga tctcggtccc gaattcttcc tcgaggctca tgatgagctc gaccaggtcc
      421 agcgagtcgg cgttgaggtc gtcgacgaac gacgcctcgg ggttgacctc tgcctcgtcc
      481 accccgagtt gctcgatgac gatcttcttc agccgatcgt acgtgctggc cactggctcc
      541 ctcctgggct tccgggtgtt gactagcggg ctgcgtccat gtcgcgggcc actcgcccga
      601 gcacgccgtt catgagccgt cgcatcggtt cgcctgagta tgtccgcgcg agctcgaccc
      661 actcggcgat cgccacccgg gccggcgttg cgggcgagtg taacacctcg cataggccgc
      721 aacgcagcag cgcgcggtcc atccgggcga gctggacgac ggggtattgg ggggcggcgt
      781 cggcgatggt ggcgtcgagc gaatcgcggt acgcgaccac gttggcgacc agctcccggg
      841 cgagcgccgc ggtgtccgcg tcgccctcca tggccaggtg ccgctcgagg atcgcgtcgg
      901 ccgtccgctg gccgaactcc gcctcgaaca ccgacgccag ggcgagccgc cggccggccc
      961 gccggctgcg gacgtgggcg tcgccggccg gatctcgcct cccgcggggg ctgtcaggct
     1021 ccgacgccat cgaccaggac ggtgacggcg ccgagctcca tgcccagcag gcgctcgacc
     1081 gccgcgccga cggcctcgcg gacctgcgcg gtgagcggga cgaggggctg ccgcggccgg
     1141 gcgacgagcc ggacggtgac gtcgacgcgg ccatcgcgga tacgaatgcg gatcggcgat
     1201 cctcccagga cgtcgcgcca gcgcggtccg ccgcgaccga cccgcaacac gcccggcacc
     1261 tcgagcgccg cgaggcggat cgtctcgcgg atcacgccgg gacccacgga cagtgatggc
     1321 gtcacgtcag cgcctccgag atgatcgcgg gcgcggcggc gacaccgggc tcgtcatcgg
     1381 cctcgccgag ggccacgccg atgcgctccg ggacgcgctc gcgcgcggtc gcggcgccga
     1441 cccggacggc gtgctcgatc atccgccgcc gggcacggcc gtgggtgatg atcacggccc
     1501 cgcgcacgcc cagcagcggg gatccaccga cgacctcgta gtcgaacacg ccgcggatgc
     1561 gcctgatgct cggccgcatc agcacgtacg cgagccggcc gcgaatcgag cccgtgaact
     1621 cggcccggaa caggtcgaag atgaaccccg acaagccctc gaagaacttg atcacgacgt
     1681 tgcccaggac cgcgtcgcag accaccacgt cggccatctg ccgggtgagg tccttgccct
     1741 cgacgttgcc cacgaagttg agatcggtct cgtcgagcag c
//
LOCUS       DBNHXO010000414         2324 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4089563, whole genome shotgun sequence.
ACCESSION   DBNHXO010000414 DBNHXO010000000
VERSION     DBNHXO010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4089563"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="AC162A_08410"
     CDS             <1..458
                     /locus_tag="AC162A_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR5010902.1"
                     /translation="GELADRVGVSTDTVRFYERSGWLPRATRQDNRYRDYAEPDVEHL
                     RLLIELRRLDVPLDDAAQIAGWCHSGHCADATAELPRLIGKRRADVEDRIAGLQALDA
                     RLAELERHLARPRRMLDVVSAGGPCCSAADAVVGSADGGCACCAPVPLS"
     gene            complement(459..1658)
                     /locus_tag="AC162A_08415"
     CDS             complement(459..1658)
                     /locus_tag="AC162A_08415"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5010903.1"
                     /translation="MDTIEIDEAAARTGSTPADLRRLIELGILTPDADGRFTTGDVRR
                     IGLVQGVVAAGIPLDGLSEAIRSGQVSMAFIDAAAFDRFVSPIDVTFAQLAEERGVPV
                     ELLTFIREAAGSGVPSPDDRVRNEELPYVDLIEMQFKAGFRTSSIQRMARVEGDSMRR
                     IAETQSAMWQHEVIEPGIAAGMRPDETLGNEFGDRMSVLSERAVVAMYHMQQTRAWTT
                     GIIEGLEAMLASAGLHTRAEHPPAMCFFDITGYTRLTQERGDEAAADLAEQLGRLVQR
                     ESVRHGGRAVKWLGDGVMLHFPNPGHGVAAALDMVHGVVDAGLPPAHVGLHAGPVIFQ
                     EGDYYGQTVNLAARIAAHADPGQVLVSQTVVDACDDEAVAFTEIGAVELKGVDGTTQL
                     FAAGRPR"
     gene            1706..2008
                     /locus_tag="AC162A_08420"
     CDS             1706..2008
                     /locus_tag="AC162A_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015656343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HIR5010904.1"
                     /translation="MDADTLDLAFTGDVISWRGPAPFYFVEVPGDLCEIIGAVSSMVT
                     YGWGVIPVQVQLGGSDWTTSLFPKEGRYLVPLKDAIRKREGLDQGDEVSVRLIIRL"
     gene            2033..>2324
                     /locus_tag="AC162A_08425"
     CDS             2033..>2324
                     /locus_tag="AC162A_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR5010905.1"
                     /translation="MSRLLVHIATGPENPTRVALGLLVARTALEAGHSVDLFIAGDGV
                     AILRPETLDAGNGIGTGSLREHVDALAAGGARFFASGMSSKARGLTAESLGGL"
BASE COUNT          319 a          850 c          800 g          355 t
ORIGIN      
        1 ttggcgagct cgcggatcgg gtcggggtgt cgacggacac ggtccgcttc tacgagcgtt
       61 ccggttggct gccgcgggcg acgcgccagg acaaccgata ccgcgactac gccgagcccg
      121 acgtcgaaca tctgcgcctc ctgatcgagc tgcggcggct cgacgtcccg ctcgacgatg
      181 cggcgcagat cgccggctgg tgccactcgg gccactgcgc cgacgcgacc gcggagctgc
      241 cgcggctgat cggtaagcgt cgggccgacg tcgaggatcg gatcgccggc ctccaggccc
      301 tcgacgcgcg cctcgccgag ctcgagcggc acctggcccg accgcgccgc atgctcgacg
      361 tcgtgagcgc cggcggtccg tgctgcagcg cagccgacgc ggtcgtcggg tcggcggacg
      421 gcggctgcgc ctgctgcgcg cccgtccctc tctcgtagct accgcggccg acctgccgcg
      481 aacagctgtg ttgtcccgtc gacgcccttg agctcgacgg caccgatctc ggtgaaggcc
      541 accgcctcgt cgtcgcaggc gtcgacgact gtctggctga ccaggacctg gccgggatcc
      601 gcgtgggccg cgatccgtgc cgcgaggttc accgtctggc cgtagtagtc gccctcctgg
      661 aagatgaccg gcccggcgtg cagcccgacg tgcgctggcg gcaggcccgc gtcgacgacc
      721 ccgtggacca tgtcgagcgc ggccgcgacc ccgtgacccg ggttgggaaa gtgcagcatc
      781 acgccgtcgc ccagccactt gaccgcgcgg ccaccgtgcc tcacggactc gcgctggacc
      841 agccggccga gctgttccgc gagatcggcc gccgcctcgt cgccgcgctc ctgggtgagc
      901 cgcgtgtagc ccgtgatgtc gaagaagcac atggccggcg gatgctcggc gcgggtgtgg
      961 aggccggcgc tcgccagcat cgcctcgagg ccttcgatga tgcccgtcgt ccatgcccgc
     1021 gtctgctgca tgtggtacat cgcgaccacg gcccgctcgg acaggacgct catccgatca
     1081 ccgaactcgt tccccagcgt ctcgtcgggt cgcatgcccg cggcgatgcc gggttcgatc
     1141 acctcgtgct gccacatcgc ggactgcgtc tccgcgatcc ggcgcatgct gtccccctcg
     1201 acccgcgcca tccgctggat cgaggacgtg cggaagccgg ccttgaactg catctcgatc
     1261 aggtcgacgt aggggagctc ctcgttgcgg acccggtcgt cagggctggg cacgcccgag
     1321 ccggccgcct cccggatgaa ggtgagcagc tctaccggca cgccgcgctc ctcggccaat
     1381 tgggcgaacg tgacgtcgat gggcgagacg aaccgatcga acgcggccgc atcgatgaac
     1441 gccatcgaca cctggccgct gcggatggcc tcgctgaggc cgtcgagcgg gatgcccgcc
     1501 gcgaccacgc cctggaccag accgatccgc cgcacatcgc ccgtggtgaa gcgaccgtcc
     1561 gcatccggcg tgaggatccc gagctcgatc aggcggcgaa ggtccgccgg ggtgcttcct
     1621 gtgcgcgcgg cggcctcatc gatctcgatc gtgtccatct tcccaactat gcgcggcgcc
     1681 gcgtcgcacc gcacactgtc gcagcatgga tgccgacacc ctggacctcg ccttcacggg
     1741 cgacgtgatc tcctggcgcg gccccgcgcc gttctacttc gtcgaggtgc ccggcgacct
     1801 gtgcgagatc atcggggccg tctcgagcat ggtcacctac ggctggggcg tgatcccggt
     1861 ccaggtccag cttggcggca gcgactggac gacctcgctg tttcccaagg agggccggta
     1921 ccttgtgccg ctcaaggacg cgatccggaa gcgcgagggc ctcgaccagg gtgacgaggt
     1981 ctcggttcgg ctgatcatcc gcctctagga tcacgacaaa ggagggccac agatgagtcg
     2041 cctgctcgtt cacatcgcca ccggacccga gaacccgacc cgggtcgccc tggggctcct
     2101 tgtcgcgcgg accgccctcg aggccgggca ctcggtcgac ctgttcatcg ccggcgacgg
     2161 cgtcgcgatc ctgcggcccg agaccctcga cgccgggaac ggcatcggga ccgggagcct
     2221 gcgcgagcac gtcgacgcgc tcgccgccgg cggtgcgcgc ttcttcgcgt ccggcatgtc
     2281 cagcaaggcg cgaggactga cggccgagag cctcggcggg ctgg
//
LOCUS       DBNHXO010000415         1918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3494396, whole genome shotgun sequence.
ACCESSION   DBNHXO010000415 DBNHXO010000000
VERSION     DBNHXO010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3494396"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1918
                     /locus_tag="AC162A_08430"
     CDS             <1..>1918
                     /locus_tag="AC162A_08430"
                     /inference="COORDINATES: protein motif:HMM:NF046269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
                     /protein_id="HIR5010906.1"
                     /translation="PDGGQPDLLFCENETNAPRIFGQPATTPWPKDGINDHVIGGAET
                     VNPDGSGSKAAAWYRLTVEPGATAEIRLRLRPRPPEIAPREEGGAIDLLGPSFTELLA
                     TREAEADAFYADLSRPGATDDEQMVMRQAFAGMLWSKQFYAYDVGRWLDGDLAGSPPP
                     SERLTGRNAGWRHFDAADILSMPDKWEYPWFAAWDLAFHAITLAHVDPAFAKYQLLVM
                     CREWFQHPNGALPAYEWSFDDVNPPVHALSALAVWNIDGRRDMEFLKRIFHKLLFNFT
                     WWLNRQDSEGNDLFSGGFLGLDNLSAFDRSHLPVAGKLEQSDATAWMYAYCVSMLSMA
                     TALAAHDPAYTDLLTTFLERAARITAAINQSGLWDETDGFYYDKLHMPDGTSVALRIH
                     SMVGLLPILPAALVPRPAAELGAALGKHFSRFLANMGVTDATLRARGSLIERPGGSSM
                     ILSLLPPIHLERVLRDALSEDAFLSPHGMRALSRRHLADPFRIEIEGYAASIDYEPGE
                     STNNLFGGNSNWRGPVWFPVNYLFIESMLRWDEALGADFTVEYPTGSGVRLRLRDVAA
                     DLSARLVSIWLPDADGHRPVAGTIAKFRDDPEWRDLLLFHEYFHGDTGAGIGASHQTG
                     WTGLVAHLLCRGGP"
BASE COUNT          284 a          706 c          636 g          292 t
ORIGIN      
        1 gtccggacgg cgggcagccc gacctcctgt tctgcgagaa cgagaccaac gcgccgcgca
       61 tcttcgggca gccggcgacg acgccgtggc cgaaggacgg catcaacgat cacgtgatcg
      121 gcggcgcgga gaccgtgaac cccgacgggt cgggctcgaa ggcggcggcc tggtaccgcc
      181 tgaccgtcga gcccggtgcg accgcggaga tccggctgcg cctgcgcccg aggccgcccg
      241 agatcgcccc gcgcgaggaa gggggcgcca tcgacctgct cggtccatcg ttcacggagc
      301 tgctcgccac ccgcgaggcc gaggccgacg cgttctacgc ggacctgagc cggcccggcg
      361 ccacggacga tgagcagatg gtcatgcgcc aggcgttcgc cgggatgctg tggagcaagc
      421 agttctatgc ctacgacgtc ggccgctggc tcgacggcga cctggcgggc agcccgccgc
      481 catccgagcg gctgaccggg cggaacgccg gctggcgcca cttcgacgcc gcggacatcc
      541 tgtcgatgcc cgacaagtgg gagtacccgt ggttcgccgc ctgggacctc gcgttccacg
      601 cgatcacgct cgcccacgtc gacccggcgt tcgccaagta ccagctgctc gtcatgtgcc
      661 gggaatggtt ccagcacccc aacggcgcgc tgcccgccta cgagtggtcg ttcgacgacg
      721 tcaacccgcc cgtccacgcc ctctcggcgc tcgcggtgtg gaacatcgac ggccgccggg
      781 acatggagtt cctcaagcgg atcttccaca agctgctgtt caacttcacg tggtggttga
      841 accgccagga ctcggagggg aacgacctgt tctcgggtgg gttcctgggc ctcgacaacc
      901 tgagcgcctt cgaccgttcg cacctcccgg tcgccggcaa gctcgaacag tcggacgcga
      961 cggcttggat gtacgcgtat tgcgtctcga tgctcagcat ggcgacagcg ctggccgccc
     1021 acgaccccgc ctacacggac ctcctcacga cattcctgga gcgcgccgcc cggatcaccg
     1081 cggcgatcaa ccagagcggc ctgtgggacg agacagacgg cttctactac gacaagctcc
     1141 acatgcccga cgggacgtcc gtcgccctcc ggatccactc gatggtgggc ctgctgccga
     1201 ttctcccggc ggcgctggtc cctcggccgg cggccgagct gggggcggcc ctgggcaagc
     1261 acttctcgcg cttcctcgcc aacatgggag tcaccgacgc cacgctccga gctcgaggct
     1321 cgctcatcga gcgacccggc ggatcgtcga tgatcctcag cctcctgccg ccgatccacc
     1381 tcgagcgggt gctgcgggac gcgctgtccg aggacgcgtt cctctcgccg cacggcatgc
     1441 gggcactctc gcgccgccat ctcgccgatc cgttccggat cgagatcgag ggctatgcgg
     1501 cgtccatcga ctacgagccg ggagagtcca ccaacaacct gttcgggggc aactccaact
     1561 ggcgcgggcc cgtgtggttc ccggtgaact acctgttcat cgagtcgatg ctgcgctggg
     1621 acgaggcgct gggcgccgac ttcaccgtcg agtacccgac gggctcgggc gtgcgcctgc
     1681 ggctgcgcga cgtggccgca gatctctccg cccggctggt ctcgatctgg ctccccgacg
     1741 ccgacggcca ccgccccgtc gcgggcacga tcgccaagtt ccgtgacgac cccgagtggc
     1801 gggacctgct cctgttccac gagtacttcc acggggacac gggcgccggc atcggcgcct
     1861 cgcaccagac cggctggacg ggactcgtcg cgcacctcct gtgccgcggc gggcccat
//
LOCUS       DBNHXO010000416         1559 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3495119, whole genome shotgun sequence.
ACCESSION   DBNHXO010000416 DBNHXO010000000
VERSION     DBNHXO010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3495119"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..>1559
                     /gene="tyrS"
                     /locus_tag="AC162A_08435"
     CDS             257..>1559
                     /gene="tyrS"
                     /locus_tag="AC162A_08435"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013400227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR5010907.1"
                     /translation="MTASAEEGASTGLGGLLAELEWRGILHATTPGLPARLATGRTIS
                     AYIGFDPTGASLHVGHLVPIFGLIRLQRAGGRPVALVGGGTGMIGDPSGRSSERNLLD
                     AATIEHNVAAIRVQLERFLDFSPGPTQAVMANNLDWLGKLSLIEYLRDVGKHFTVPYM
                     LAKDSVQQRLDRGLSFTEFSYMTLQAFDFATLYREHGVEMQMGGADQWGNITAGLELI
                     RRTSGDAEESPAHGIAYKLLLSASGAKFGKSESGDSVWLDPARTTPFAFYQYWVQADD
                     RDAGTYLRWFTELPRERVEALDAETAAHPELRAAQKALALDVTTRVHGEAAAADAIRV
                     SEALFQREPITDPALLASVHEATRGPSVAAGESGVVALLADTGLAPSRGEARRLILGG
                     AITVNGERIADPAAPLPPAIDGAWFEVRVGKRQRAVVRVSPG"
BASE COUNT          219 a          545 c          580 g          215 t
ORIGIN      
        1 acggaggccg tcgcaccggc gacgcccacc acggcaagga caaagaacag gaagaggggg
       61 atcgctaccg cgacggcctt ggccgcggtg gcaccgctcc cgcgagggcg gctgcggtca
      121 ccgagccggc gacggcgctg cctccggaga ctggtctgca tgactcgcgt gaagatacca
      181 tgcgggggat ggacgcccgt ttctgccgac cgggccggag gatcgacggt cccggccgcc
      241 agcctgtgac gcccgcgtga cggcgtcggc cgaggaaggg gcctcgaccg ggctcggcgg
      301 gctgctcgcg gagctcgagt ggcgcggaat cctgcatgcg acgacgccgg gcctgcccgc
      361 gcggctcgcc acgggacgca cgatctcggc gtacatcggg ttcgacccga cgggggcgtc
      421 gctgcacgtc ggccacctgg tgccgatctt cggcctgatc cggctccagc gggccggcgg
      481 gaggcccgtc gccctggtcg gcggcggcac cgggatgatc ggcgacccgt cggggcgctc
      541 gtccgagcgc aacctcctcg atgcggccac gatcgagcac aacgtggccg cgatccgggt
      601 tcagctggag cgcttcctgg acttttcgcc gggtcccaca caggccgtga tggcgaacaa
      661 cctcgactgg ctgggcaagc tctcgctgat cgagtacctg cgcgacgtcg gcaagcactt
      721 caccgtgccc tacatgctgg ccaaggactc ggtgcagcag cggctcgacc gcgggctgtc
      781 gttcaccgag ttcagctaca tgaccctcca ggcgttcgac ttcgcgaccc tgtatcgcga
      841 gcacggcgtc gagatgcaga tgggcggcgc ggaccagtgg ggcaacatca cggcggggct
      901 cgagctcatc cgccgcacct cgggcgacgc ggaggagagc ccggcccacg gcatcgccta
      961 caagctcctg ctgtcggcgt cgggcgcgaa gttcggcaag agcgagtcgg gcgattcggt
     1021 ctggctggat cccgcgcgca cgacgccgtt cgcgttctac cagtactggg tgcaggcgga
     1081 cgaccgcgac gccgggacgt atctgcgctg gttcactgaa ctgccgcgcg agcgggtgga
     1141 ggcgctggac gccgagacgg ccgcccatcc cgagctgcgc gccgcgcaga aggcgctcgc
     1201 gctcgacgtc acgacgcggg tccatggcga ggcggccgcg gcggatgcga ttcgcgtgtc
     1261 cgaggcgctg ttccagcgtg agccgatcac cgatcccgcc ctcctggcga gcgtccacga
     1321 ggcgacccgg gggccatcgg ttgccgcggg cgaaagcggg gtcgtcgccc tgctcgccga
     1381 cacgggactg gccccgtcgc gcggcgaggc ccggcgcctg atcctgggcg gggcgatcac
     1441 cgtgaacggc gagcgcatcg ccgacccggc ggcgccgttg ccgccggcta tcgacggcgc
     1501 ctggttcgag gttcgcgtgg gcaagcgcca gcgggccgtc gtccgggtct cgcctggct
//
LOCUS       DBNHXO010000417         1797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2504356, whole genome shotgun sequence.
ACCESSION   DBNHXO010000417 DBNHXO010000000
VERSION     DBNHXO010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2504356"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="AC162A_08440"
     CDS             <1..660
                     /locus_tag="AC162A_08440"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF013155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HIR5010908.1"
                     /translation="TGATVRLICTALRSHDPAANLELAETAARFRDRGLTGWDLAGPE
                     AAYPDPMLHATAFEAARAGGLRITLHAGEWGGAAQVRRALGMDPERIAHGPGAIDDPA
                     LCADLIARDVSLDLCPSSNWQAGIVPSVAAHPLARLHRAGVPVTLSTDDTTVSDITLS
                     EEYGHAVEGIGLTWPELWTSDRRALDVASADEADLAPLRTTFDAWAAGVPELDAHAFS
                     G"
     gene            702..>1797
                     /locus_tag="AC162A_08445"
     CDS             702..>1797
                     /locus_tag="AC162A_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5010909.1"
                     /translation="MHCPACGTANEAGRKFCHECGSRLAATCPSCGSPNTPGARFCGE
                     CGSKLADGGTAAVGTPVGPGVATPRPLPDGSGPVAERRLVSILFADLVGFTALSEGRD
                     AETVRELLSAYFDLSREVIARHGGTVEKFIGDAVMAIWGAPVAHEDDAERAVRAALEL
                     VDAVRALGQEVHARAGVLTGEAAVTLGATDQGMVAGDLVNTASRLQSVAAPGTVLVGE
                     TTQRAASGSIAFEPAGDQVLKGKEAPVPAWRALRVVAERHGRGRSDRLEAPFVGRDAE
                     LRLLKDLFHATSKERRARLVSITGQAGIGKSRLAWEFEKYVDGVVEKVMWHDGRSPAY
                     GEGITFWALGEMVRGRTGLIEGEDPETTRVK"
BASE COUNT          237 a          639 c          680 g          241 t
ORIGIN      
        1 accggcgcca cggtccgcct gatctgtacg gcgctccgct cgcacgaccc ggccgcgaac
       61 ctcgagctcg ccgaaacggc ggcgcggttt cgcgatcgcg ggctgaccgg ctgggacctc
      121 gcgggacccg aggcggcgta cccggacccg atgctgcacg ccactgcctt cgaggcggcg
      181 cgggccggcg ggctgcggat caccctgcac gccggagagt ggggcggggc cgcgcaggtc
      241 cggcgcgccc tcgggatgga ccccgaacgg atcgcccatg gcccgggcgc gatcgacgac
      301 ccggccctct gcgccgacct gatcgcgcgg gacgtctcgc tggacctgtg cccgagctcg
      361 aactggcagg ccggcatcgt cccgagcgtg gccgcccacc cattggccag gctccaccgg
      421 gcgggcgtcc cggtcacgct ctccaccgat gacacgaccg tctcggatat cacgctctcc
      481 gaggaatacg ggcacgcggt cgaggggatc gggctgacct ggcccgagct gtggaccagc
      541 gaccgccggg cgctcgatgt cgcgtccgcg gacgaggcgg atctggcgcc cctgcgaacc
      601 acgttcgatg cgtgggccgc gggggtccct gagctcgatg cccacgcctt cagcggttga
      661 cgaggcgaca atcggacacg cgggcgcggt acattccacc catgcactgc ccggcgtgtg
      721 gcacggccaa cgaggccggc cgcaagttct gccacgaatg cggctcccgg cttgctgcca
      781 cgtgcccgtc ctgcggctcg ccgaacaccc ccggggcgcg gttctgcggc gagtgcgggt
      841 ccaagctggc ggacggcggg acggcggcgg tcggaacacc ggtcgggcct ggggtcgcga
      901 cgccgcgccc cctccctgat gggtcgggcc cggtcgccga gcgccggctg gtcagcatcc
      961 tgttcgccga cctcgtgggg ttcaccgccc tttcggaggg ccgggatgcg gagacggtcc
     1021 gcgaactcct atcggcctac ttcgacctgt cgcgcgaggt catcgcccgc cacggcggca
     1081 ccgttgagaa gttcatcggt gatgccgtca tggcgatctg gggcgcaccc gtggcacacg
     1141 aggacgacgc ggagcgggcc gtgcgggccg ccctcgagct cgtcgatgcc gtgcgagccc
     1201 tcggccagga ggtccatgcc cgcgcgggcg tcctgacggg cgaggccgcc gtgacgctgg
     1261 gggccaccga ccaggggatg gtcgcaggcg acctcgtgaa cacggcctcg cggctgcaat
     1321 cggtcgccgc acccggtacg gtcctcgtcg gcgagacgac gcagcgggcc gcgtcgggtt
     1381 cgatcgcgtt cgagccggcc ggtgaccagg tcctgaaggg caaggaggcc ccggtccccg
     1441 cctggcgggc gctgcgggtc gtggccgagc ggcacggccg cggccggtcg gaccgtctcg
     1501 aggcgccgtt cgtcggccgc gacgccgagc tacggctgct caaggacctc ttccacgcga
     1561 cctcgaagga gcgccgcgcg cggctcgtct cgatcacggg gcaggccggc atcgggaagt
     1621 cccggctggc ttgggagttc gagaagtacg tcgacggcgt cgtcgagaag gtcatgtggc
     1681 atgacggccg atccccggcg tacggcgagg gcatcacgtt ctgggcgctc ggcgagatgg
     1741 tccgcgggcg gacggggctc atcgaggggg aggacccgga gacgacccgc gtgaagg
//
LOCUS       DBNHXO010000418         1817 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3693735, whole genome shotgun sequence.
ACCESSION   DBNHXO010000418 DBNHXO010000000
VERSION     DBNHXO010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3693735"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /gene="surE"
                     /locus_tag="AC162A_08450"
     CDS             complement(<1..517)
                     /gene="surE"
                     /locus_tag="AC162A_08450"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR5010910.1"
                     /translation="MTRERARSERRDLDKERGEPVAGSRGSKRTLKRVGRPLGDWTTR
                     PRILVTNDDGIESRGILALKQALDPIGDVTVVAPDTNQSAVGHQKTLMRPLRVRERTL
                     ADGSSGYSIDGSPTDAVSLAFLGFFEEGFDLVASGINYGANLGDDITYSGTVSAAMEA
                     VINNCPAFAISQ"
     gene            complement(562..1803)
                     /locus_tag="AC162A_08455"
     CDS             complement(562..1803)
                     /locus_tag="AC162A_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR5010911.1"
                     /translation="MPDGLTERQRWLIDAALVLAVIALGFVVLGFAANVFYAFGDVLL
                     LFFLAWLLSFALLPLINGLTRLVPRLSQAGAVIVVYLTIVFVLLAILVQASATLASSI
                     NQFIQDAPNLEEQLTNLLTELQARLQGFGFHVDLVGQAPQIITNLQQWAAQLVGPLQS
                     VAVASIGVFGSILILVILSIYIAVDRDEIVAFLYRLVPPGLVTEARLLQTSTSKSFGG
                     FLRGQVVMGLFFGLLTAIVNIVFGLPYAAVTTVAAGLLQMIPFFGPFVSWLPPVAVAV
                     LLKPEVTLPVLIVMGIAWFVTMNVISPRLMSGAVGIHPIVVLASVVIGGKIAGIVGAI
                     FGIPIAAVLSAFFFYWFGRSREGGSVADRATQRVAAREGRPVRRPREPVPGVDADVDD
                     VAGARPAPTLAEAAEQAEPGA"
BASE COUNT          310 a          637 c          637 g          233 t
ORIGIN      
        1 cctgcgagat cgcgaacgcc gggcagttgt tgatgaccgc ctccatcgcg gccgacaccg
       61 tgcccgagta ggtgatgtcg tcgcccaggt tcgcgccgta gttgatgccc gaggcgacga
      121 ggtcgaagcc ctcctcgaag aagccgagga acgcgaggct gacggcgtcg gtgggcgagc
      181 cgtcgatcga gtagcccgac gacccgtcgg ccagggtccg ctcgcgcacc cgcaggggcc
      241 gcatcagcgt cttctggtgc ccgaccgcgg actggttcgt gtccggcgcg accaccgtca
      301 cgtcgccgat cgggtccagc gcctgcttga gcgcgaggat gccgcgcgac tcgatgccgt
      361 cgtcgttggt gacgaggatg cgcggccgtg tcgtccagtc gccgagcggc cgcccgaccc
      421 gcttgagcgt ccgcttgctg ccgcgcgacc cggcgaccgg ctcgccgcgc tccttgtcca
      481 ggtcgcgccg ctcggatcgg gcgcgctccc gggtcatgtc agggacgccc tcaccggatc
      541 gcccgggtcc gccgtcctcg ctcacgcgcc gggctcggcc tgctccgcgg cctcggccag
      601 ggtcggcgcg gggcgtgcgc ccgcgacgtc gtccacgtcg gcgtcgacgc ccggtacggg
      661 ctcccgtggc cgcctgaccg gccggccctc gcgggcagcc acccgctggg tcgcgcgatc
      721 cgcgaccgac cccccttctc gcgaccggcc gaaccagtag aagaagaacg ccgacagcac
      781 cgcggcgatc gggatcccga agatcgcgcc gacgataccc gcgatcttgc cgccgatcac
      841 caccgacgcc aggacgacga tcgggtggat gccgacggcg cccgacatca ggcgcggcga
      901 gatcacgttc atcgtcacga accacgcgat gcccatcacg atcaggacgg gcagcgtgac
      961 ctcgggcttc agcagcacgg ccacggcgac cggggggagc caggacacga acgggccgaa
     1021 gaacgggatc atctgcagca gtccggccgc caccgtggtc accgccgcgt agggcaggcc
     1081 gaacacgatg ttgacgatcg ccgtgagcag gccgaagaac aggcccatca cgacctggcc
     1141 ccgcaggaac ccgccgaatg acttcgacgt gctcgtctgc agcagccgcg cctcggtcac
     1201 cagaccgggc gggaccaggc ggtacaggaa cgcgacgatc tcatcgcgat cgacggcgat
     1261 gtagatcgac aggatcacca ggatcaggat gctgccgaag acgccgatgc tggccacggc
     1321 gacggactgg agcggcccga ccagctgcgc ggcccactgc tggaggttgg tgatgatctg
     1381 cggggcctgg cccaccaggt cgacgtggaa cccgaatccc tggagccgcg cctggagctc
     1441 ggtcagcagg ttggtcagct gctcctcgag gttcggagca tcctggatga actggttgat
     1501 cgacgacgcc agcgtcgccg aggcctggac gaggatcgcg agcaggacga acacgatcgt
     1561 gaggtagacg acgatcacgg cgcctgcctg ggacaggcgc ggcacgagtc gcgtcagccc
     1621 gttgatcagg ggcagcagcg cgaacgacag cagccaggcg aggaagaaca gcagcaggac
     1681 gtcgccgaac gcgtagaaca cgttggcggc gaacccgagc acgacgaacc cgagggcgat
     1741 caccgcgagg acgagcgcgg cgtcgatcag ccaccgctgg cgttccgtca ggccgtcggg
     1801 catggcgtca gcctaca
//
LOCUS       DBNHXO010000419         6014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_719734, whole genome shotgun sequence.
ACCESSION   DBNHXO010000419 DBNHXO010000000
VERSION     DBNHXO010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_719734"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="AC162A_08460"
     CDS             <1..768
                     /locus_tag="AC162A_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HIR5010912.1"
                     /translation="WSLIQMIGRAARNTGGEVVMYADRITESMRIAIDETDRRRAIQE
                     RHNAEHGIEPQTIIKGIHDINDRLRAVAENTVMYSSERRERQLADADKAEVERLVAQM
                     EAEMKAAAKQLEFERAAALRDEIQQIRLRVLEEDASVVVARAAERAARDAALPAKERA
                     AAHRPGGAGLPTGAQSFEVTEVTVLPSGEEPASAIDEDGSTGVAAELFPGIRDEHDDD
                     DGGWQARWLDRPTWDRTVTPNIRRRTGGRPTRRARGY"
     gene            866..1423
                     /locus_tag="AC162A_08465"
     CDS             866..1423
                     /locus_tag="AC162A_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010913.1"
                     /translation="MNSLPRSRRAVARLLSSLALVLLAVAACGGSSPLGGGGDPASVV
                     KDALAVVQSKDLAKIGDLACAAKKDEVVESLDFSKGMEGSGVDSQQVLDAMSIDTSKV
                     TVGTPVINGDTATVPLSGSMTMSVDPAKMKPIVKAQLEAEGLPADDAAVDAMMGMVSG
                     FTGQDIPMDQSMTLVKENGAWKICE"
     gene            1545..4619
                     /gene="uvrA"
                     /locus_tag="AC162A_08470"
     CDS             1545..4619
                     /gene="uvrA"
                     /locus_tag="AC162A_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR5010914.1"
                     /translation="MPQDRIVVRGAREHNLKNVDVAFPRDRLVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMEKPDVDQIDGLSPAISIDQKGASRNPRSTVGT
                     VTEIYDYLRLLFARVGHPHCPKCGREIERQQISQMVDQILALPDGTRLLVLGPLIKDR
                     KTEGDRVFEGARKQGFVRVRVDGEQYDLSDAPTLDRYKRHTIEVVVDRFVVSRAEAPE
                     TWERDAAGRPIDPETGLALRDPDASRLADSIETALRLGEGVAVVAPAPRDGEGTTFEE
                     RRFSERYSCPYDGTMIDELEPRSFSFNSPHGACPTCTGLGTQLVIDPELVIPDRSKSI
                     RAGALVPWNKMPTEISWRMKITEAIFTSRGWRIDTPVRDLPEEAIEYLLYAPKDEKVV
                     VRYRHERGENSYVATFEGVVTNLERRFKETESEYIRTELEKFMVQKPCPTCKGRRLRP
                     EALGVTVDGRNISEVATLSVTDALDWAAGLGALITERERTIARQVLKEIVARLGFLVD
                     VGLDYLTVDRASVSLSGGEAQRIRLATQIGTTLMGVLYILDEPSIGLHQRDNAKLIAT
                     LTRLRDLGNTVLVVEHDEETIRTADWVIDIGPGAGEHGGEIIANGTLDALLAEPRSIT
                     GAFLRGERQVPIPARRRHGNGRALVVKGARQHNLRGIDVRFPLGTFISVTGVSGSGKS
                     TLVDEVLYRALARDLTGSREIVGAHDAVEGVEHIDKIIEIDQSPIGRTPRSNPATYTG
                     LFGPIRELFAGTPDARVRGYQPGRFSFNVKGGRCENCKGDGIIKIEMQFLPDVYVPCE
                     VCKGKRYTREALEIHYKGRSIADVLEMTVEEANEFFSAVPTVKAKLQTLFDVGLGYIH
                     LGQPATTLSGGEAQRVKLATELSKRATGKTLYLLDEPTTGLHFADVDKLLQVLHRLVD
                     NGNTVLVIEHNLDVIKTSDWIVDLGPGGGHRGGLVIAEGTPEAVAETPGSATGEYLAR
                     VLRGEPLVPLSDVTFADEAGRPGVGGNGHDAAAIDIAPSPKRVAAAPSAGDR"
     gene            4681..5118
                     /locus_tag="AC162A_08475"
     CDS             4681..5118
                     /locus_tag="AC162A_08475"
                     /inference="COORDINATES: protein motif:HMM:NF046506.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6941 family protein"
                     /protein_id="HIR5010915.1"
                     /translation="MPEIDYAFLADAAETQPGQKFHVLGGGITRIGGRTFPLRHPHVA
                     LVVGLLVTAPETDREHELRIVLLDPDGHEVTGATGSLQARGHPDGRDAILTFSIDLWN
                     LTFPSPGDYSFRILVNGSERKRLALVLVRPADSGVASASLSDL"
     gene            5115..5462
                     /locus_tag="AC162A_08480"
     CDS             5115..5462
                     /locus_tag="AC162A_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010916.1"
                     /translation="MTTAREGRDAASRGAARHLYPAIDEAGLVAARACDRLVAAARTA
                     EAARWLAYFEPIPDRLRDDAIGDLRRTALRARAAYGPKDSVRDVLPEDVTEPFLDAID
                     RLLKALAKREVGG"
     gene            complement(5459..>6014)
                     /locus_tag="AC162A_08485"
     CDS             complement(5459..>6014)
                     /locus_tag="AC162A_08485"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5010917.1"
                     /translation="TPMLAHGSIYLRAAERADIPLFVAWFNDYATSRTLSIRAPMSSA
                     SEEQWFEHALADQGKSGYHFVACLIGDDRPIGTIGFFDLDHVNGSAALGLSIGSAPDR
                     GKGHGSAMIEALLRFGFASLRLERIELDCYDFNADAHRLYQRLGFVDEGVARHRVFRE
                     GRHVDLFKMSMLAGEWRATRGSES"
BASE COUNT          956 a         2126 c         2119 g          813 t
ORIGIN      
        1 tggtccctga tccagatgat cggccgggcg gcgcgcaaca cgggcggcga ggtcgtgatg
       61 tacgcggacc ggatcacgga gtcgatgcgg atcgcgatcg acgagaccga ccgccggcgc
      121 gcgatccagg agcgtcacaa cgccgagcac gggatcgagc cccaaacgat catcaagggc
      181 atccatgaca tcaacgaccg gctgcgcgcg gtcgccgaga acacggtcat gtacagctcc
      241 gagcgtcgcg agcgccagct cgccgacgcc gacaaggccg aggtcgagcg gctcgtggcg
      301 cagatggagg ccgagatgaa ggccgccgcg aagcagctcg agttcgagcg cgcggcggcc
      361 ctgcgcgacg agatccagca gatccggttg cgagtgctcg aggaagatgc gtccgtggtc
      421 gttgcgcggg ccgcggagcg ggcggcccgc gacgcggcgc tgccggcgaa ggagcgcgcg
      481 gccgcgcatc gccccggcgg cgccgggctg ccgaccggcg cgcagtcgtt cgaggtcacc
      541 gaggtcacgg tgctcccctc gggcgaggag cccgcgagcg cgatcgacga ggacggctcc
      601 accggcgtcg ccgcggagct gttcccgggc atccgcgacg agcatgacga cgacgatggc
      661 ggctggcagg cgcgctggct ggaccggccg acctgggacc ggaccgtgac cccgaacatc
      721 cggcgtcgga ccggcggacg cccgacgcgt cgagcccgcg ggtattgatc gccgtctgac
      781 ggtgcgcgtg cggtggcgcg gccggaccat ggaggcgcgc gaaatcgggt cgcaatcggg
      841 cgtcccaagg cgtaggctgg cgcccatgaa cagcctgccc cgttcccgcc gagccgtcgc
      901 gcggctcctg tcgtcgttgg ccctcgtcct gcttgccgtc gccgcctgcg gcggatcgag
      961 cccgctcgga ggcggcggcg accccgcgtc cgtcgtcaag gacgccctgg ccgtcgtcca
     1021 gtccaaggac ctcgccaaga tcggcgacct cgcctgcgcc gccaagaagg acgaggtcgt
     1081 cgagtcgctc gacttctcca agggcatgga gggatcgggt gtcgacagcc agcaggtcct
     1141 cgacgcgatg tcgatcgaca cgtcaaaggt gaccgtcggc acgccggtca tcaacggcga
     1201 cacggcgacc gtcccgctca gcggttcgat gacgatgagc gtcgatccgg cgaagatgaa
     1261 gccgatcgtg aaagcccagc tcgaggcgga gggcctgccc gcagacgacg ctgcggtcga
     1321 cgcgatgatg ggcatggtca gcgggttcac gggccaggac atcccgatgg accaatccat
     1381 gacgctcgtc aaggagaacg gggcctggaa gatctgcgag tgaccccgca cccggcctcc
     1441 tagcgccagg cacggccccg ggcaccgtcg cgaggcgggt gttcggggct tcttgcagac
     1501 acgaacacct gttccatttc gcatcgatcc ggtagactgg gcgcgtgccc caggatcgga
     1561 tcgtcgtgcg cggagcgcgc gagcacaacc tcaagaacgt cgacgtcgcg ttcccccgtg
     1621 atcgcctcgt cgtcatcacc gggctgtccg gcagcggcaa gagcagcctc gcgttcgaca
     1681 ccatctacgc ggagggccag cggcggtacg tcgagagcct gagcgcctac gcgcgccagt
     1741 tcctgggcca gatggagaag ccggacgtcg accagatcga cgggctgtcg ccggcgatct
     1801 ccatcgacca gaagggcgca tcgcgcaacc cgcggtcgac ggtcggcacc gtgaccgaga
     1861 tctacgacta cctgcggctg ctgttcgcgc gcgtcggcca tccgcactgc cccaagtgcg
     1921 ggcgcgagat cgagcgccag cagatcagcc agatggtcga tcagatcctc gcgctgcccg
     1981 atggcacacg gctgctggtc ctcggtccgc tgatcaagga ccgcaagacc gagggtgacc
     2041 gcgtgttcga gggcgcccgc aagcaggggt tcgtgcgcgt ccgggtggac ggcgagcagt
     2101 acgacctgag cgacgcgccc acgctcgacc ggtacaagcg ccacacgatc gaggtcgtgg
     2161 tcgaccggtt cgtcgtcagc cgtgccgagg ccccggagac gtgggagcgg gatgcggcgg
     2221 gacggccgat cgatccggag acgggcctgg cgctccggga cccggacgcc tcgcgcctcg
     2281 ccgactcgat cgagaccgcg cttcggctgg gcgagggcgt cgccgtcgtc gccccggcgc
     2341 cgcgcgacgg cgaggggacg acgttcgagg agcggcggtt cagcgagcgc tacagctgcc
     2401 cctatgacgg gacgatgatc gacgagctcg agccgcgcag cttctcgttc aactcgccgc
     2461 acggcgcctg ccccacgtgc accggactcg gcacgcagct ggtcatcgac ccggagctcg
     2521 tgatcccgga ccggtcgaag agcatccggg ccggcgccct cgtgccgtgg aacaagatgc
     2581 cgaccgagat ctcgtggcgg atgaagatca cggaggccat cttcacctcg cgcggctggc
     2641 ggatcgacac cccggtgcgc gacctgcccg aggaggcgat cgagtacctg ctctatgcgc
     2701 cgaaggacga gaaggtcgtc gtccgctacc gccacgagcg cggcgagaac agctacgtcg
     2761 ccacgttcga gggcgtcgtc acgaacctcg agcgccggtt caaggagacc gagtccgagt
     2821 acatccggac cgagctcgag aagttcatgg tccagaagcc atgtcccacg tgcaagggac
     2881 gccggctgcg gcccgaggcg ctgggcgtca cggtcgacgg acgcaacatc tccgaggtcg
     2941 cgacgctctc ggtgaccgat gcgctggatt gggcggcggg gctcggcgcc ctgatcacgg
     3001 agcgcgagcg gaccatcgcg cgccaggtcc tcaaggagat cgtggcgagg ctgggcttcc
     3061 tggtcgacgt cggcctcgac tacctgaccg tcgatcgggc cagcgtcagc ctgtcgggcg
     3121 gcgaggcgca gcggatccgc ctagcgaccc agatcgggac cacgctgatg ggcgtcctgt
     3181 acatcctcga cgagccttcg atcgggctcc accagcggga caacgcgaag ctgatcgcaa
     3241 cgctgacccg cctgcgcgac ctgggcaata ccgtcctcgt cgtcgagcac gacgaggaga
     3301 cgatccggac cgcggactgg gtcatcgaca tcgggcctgg cgccggcgag cacggcggcg
     3361 agatcatcgc caatggcacg ctcgatgcgc tgctcgcgga gccgcgctcg atcaccggcg
     3421 ccttcctgcg cggtgagcgc caggtcccca tcccagcccg ccggcgccat ggcaacggcc
     3481 gggcgctggt ggtcaagggc gcgcgccagc acaacctgcg cggcatcgac gtccgcttcc
     3541 cgttgggcac ctttatctcc gtgaccgggg tctcgggttc cggcaagagc accctcgtcg
     3601 acgaggtcct gtaccgcgcc ctcgcccgcg acctgacggg ttcgcgcgag atcgtcggcg
     3661 cccatgacgc cgtcgaaggc gtcgagcaca tcgacaagat catcgagatc gaccagagcc
     3721 ccatcggccg cacgccgcgc tccaacccgg ccacctacac gggcctgttc gggccgatcc
     3781 gcgagctgtt cgccggcacg ccggacgcgc gggttcgcgg ctaccagccg ggccggttca
     3841 gcttcaacgt caagggcggc cggtgcgaga actgcaaggg cgacgggatc atcaagatcg
     3901 agatgcagtt cctccccgac gtctacgtcc cgtgcgaggt gtgcaagggc aagcgctaca
     3961 cgcgcgaggc gctggagatc cactacaagg gccgctcgat cgccgacgtg ctcgagatga
     4021 ccgtcgagga ggcgaacgag ttcttttccg cggtcccgac ggtgaaggcc aagctccaga
     4081 ccctgttcga cgtgggcctc ggctacatcc atctgggcca gccggcgacc acgctgtccg
     4141 gcggcgaggc gcagcgggtc aagctcgcca cggagctgtc gaagcgggcc accggcaaga
     4201 cgctctacct gctcgacgag ccgacgaccg gcctccactt cgccgacgtc gacaagctgc
     4261 tccaggtcct ccaccgcctc gtcgacaacg gcaacaccgt gctcgtgatc gagcacaacc
     4321 tcgacgtgat caagacctcc gactggatcg tcgacctcgg gccggggggc ggccaccgcg
     4381 gaggcctggt cattgccgag ggcacgcccg aggccgtcgc cgagacgccg ggttcagcga
     4441 ccggcgagta cctcgcccgg gtcctgcgcg gcgagccgct cgtcccgctg agcgacgtga
     4501 cgttcgcgga cgaagccggg cggccgggcg tcggcggcaa cggtcatgac gcggcggcga
     4561 tcgatatcgc cccgtcgccc aagcgcgtgg cggcggcgcc gtccgcaggc gatcgctagc
     4621 ggcccgcccg cgcgccgcga tccggtcgtc accccctgcg acaccctcta ggatgggccc
     4681 atgccggaga tcgactacgc cttcctcgcc gatgccgcgg agacgcagcc tggccagaag
     4741 ttccacgtcc tcggcggcgg catcacgcgc atcggcgggc ggaccttccc gctccgccac
     4801 ccgcacgtgg cgctggtcgt cgggctcctg gtgaccgcgc cggagaccga ccgtgagcat
     4861 gagctgcgga tcgtgctgct cgaccccgac ggccacgagg tgacgggcgc gacgggcagc
     4921 ctccaggcgc gcggccaccc cgacgggcgg gacgcgatcc tgaccttctc catcgacctc
     4981 tggaacctga ccttcccgtc gcccggcgac tactcgttcc ggatcctcgt caacggctcc
     5041 gagcgcaagc gcctggcgct tgtcctcgtc cggccggcgg actcgggggt cgcgagcgcg
     5101 tcgctctcgg acctgtgacg accgcccgag agggccgcga cgccgcgtcc cgcggcgcgg
     5161 cgcgccacct gtatcccgcc atcgacgagg cgggcctcgt cgcggcccgc gcctgcgatc
     5221 gcctcgtcgc ggccgcgcgc accgccgaag ccgcccgctg gctcgcctac ttcgagccga
     5281 tcccggaccg gctgcgggac gatgccatcg gcgatctccg gcggaccgcg ctccgggcgc
     5341 gggcggcgta cgggccgaag gactccgtcc gggacgtgct gcccgaggac gtcacggagc
     5401 cgttcctcga cgcgatcgac cggctgctca aggcgctcgc gaagcgcgag gtcggcggct
     5461 aggactcgga cccgcgggtc gcccgccact cgccggcgag catcgacatc ttgaacaggt
     5521 cgacgtgccg gccctcgcgg aagacccggt gccgcgcgac cccttcgtcg acgaatccga
     5581 gccgctggta gaggcggtgc gcgtcagcgt tgaagtcgta gcagtcgagc tcgatccgct
     5641 cgaggcgcag gctcgcgaac ccgaagcgga gcagcgcctc gatcatggcc gaaccgtggc
     5701 ccttgccccg gtccggggcc gagccgatcg acagcccgag cgccgcgctc ccgttaacgt
     5761 ggtcgaggtc gaaaaacccg atggtcccga tgggccggtc gtctccgatc aggcaggcga
     5821 cgaagtggta gccactcttg ccctggtcgg cgagcgcgtg ctcgaaccac tgctcttcgc
     5881 tcgccgagga catcggcgcg cggatcgaga gcgtccgcga cgtcgcgtag tcgttgaacc
     5941 aggcgacgaa cagcgggatg tcggcgcgct cggcggcgcg gaggtagatc gagccgtggg
     6001 cgagcatcgg cgtc
//
LOCUS       DBNHXO010000420         7077 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_720037, whole genome shotgun sequence.
ACCESSION   DBNHXO010000420 DBNHXO010000000
VERSION     DBNHXO010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7077)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7077
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_720037"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="AC162A_08490"
     CDS             complement(<1..269)
                     /locus_tag="AC162A_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HIR5010918.1"
                     /translation="MTPAPWADPELTSRGRLPMHAVTHDDRLPLDGRWRFQLLHRPDE
                     APGADWGEADVPGCWTMQDTWDHPIYTNVQMPFPHRPPETPEENPT"
     gene            complement(311..1231)
                     /locus_tag="AC162A_08495"
     CDS             complement(311..1231)
                     /locus_tag="AC162A_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006379287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5010919.1"
                     /translation="MTTVVTPELLAADAAVPRKRRPTPWGRIAMYVFLSLMAIVWLFP
                     LAWAIYTALRPYSDTQAAGYISLPQTLNFQNFIDAWNGGDFAQHFLSTLIVTIPAVIV
                     TLIVASMVAFSVSRFSWRFNLVILLIFTAGNLLPPQVIIVPLFKLYTALRGIPPPLSD
                     NALLYNQYFGIFLINFVFQLGFCTFVLSNYMKTISHELTEAALVDGASVLRIWGGVIM
                     PLCRPALAALATLLFTFIYNDFFWALILIKDGDRRPITSALNNLQGQFFTDTNLLAAG
                     ALLAAIPTILVFLALQKHFISGLSLGATKG"
     gene            complement(1228..2169)
                     /locus_tag="AC162A_08500"
     CDS             complement(1228..2169)
                     /locus_tag="AC162A_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003958455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5010920.1"
                     /translation="MAQPGAQHVTTGRFRRRYSLLTGRDKLVLGLMVGIPLALDLIFI
                     WGPTIVSFFLSFTNWNGIGSPFDAKVIGITNYQNLFTAYPFFWPALAHNLIWLAWLMF
                     IATPIGILFAVLLDRVGRGTRFYQTVFYLPVVLSLAVIGIIWKLQYAPEQGFINNVLG
                     RTANNNLIDWLGDPSLNLWAALVATSWRHVGYIMVLYLAGLKAFDPTLREAAKVDGAN
                     ERQTFFRVVFPVMMPINVVIVVITTIEALRAFDIAYIINRGKNGLELLSTLITNNAIS
                     ESNRLGFGSAIAVVLLVISLGPIVAFLTRMMRDQGQR"
     gene            complement(2286..3686)
                     /locus_tag="AC162A_08505"
     CDS             complement(2286..3686)
                     /locus_tag="AC162A_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5010921.1"
                     /translation="MDDFDGLRSVTRRQVLKGGAAVAALGGVSAFLAACSTPAASSAP
                     SAAATQPPAATPAATEAASVEAPSTGASGSVTLGSNYSDAIPKGVLAGIVDTFTTTTG
                     IPVKINTTDHGTFQDQISSYLQGTPDDVFTWFSGFRMRFFAEQGLATDISDVWAKIGA
                     NYSDAFKVGSTGNDGKQYFIPIYNYPWAVFYRKSMFADKGYTIPTTLDEFKALATKMQ
                     TDGLIPMAFGDSDGWPAMGTFDIINLRQNGYDFHVGLMAGEQKWTDPKTKAAFETWKT
                     LLPFYQEGAAGRTWQDAAQALVQKKAGMYLLGMFVSQQFQEAGDADLADLDFFPYPNF
                     GTEYDAEKALDAPIDGFMISANSPTLAADLDASKAFMEYLAQPATQVAWVSQDKGNIA
                     AAKDADTSGYTDLQKKAAELIGGAQRITQFLDRDTNPNFAGPNGMQAFLLDFLKNPNQ
                     DLDAFLAKIQSFWDTL"
     gene            3926..4777
                     /locus_tag="AC162A_08510"
     CDS             3926..4777
                     /locus_tag="AC162A_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR5010922.1"
                     /translation="MLARQRQALILDRVRESGAVRGADLARELEVSDMTVRRDLEILH
                     DHGLLEKVHGGATAISGLASYEPGFVAKSALQQDEKAAIAAEAARLVEPGMAIGISAG
                     TTTHTLAARVADIPGVTVVTNSLRVAEVLYRSGRRDQTVILTGGVRTPSEALVGSFAV
                     AQLRSVHLDLVFMGVHGMDGKAGFTCPNLSEADTDRALIEAGRRLVVLADHTKWGVIG
                     IASIARLDQADVLVSDTLLDPAAQSTLREVVGEVVLVDAGEPADGGNPVGLDDADERL
                     QAAGRAH"
     gene            4767..5825
                     /locus_tag="AC162A_08515"
     CDS             4767..5825
                     /locus_tag="AC162A_08515"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HIR5010923.1"
                     /translation="MPTEEQPRPSDGAVQALAAEPHRRHNALTGEWVLVSAGRTDRPW
                     LGAEEPEPPEERPTYDPGCYLCPGNRRVKGDVNPHYDQTFVFTNDFAALRPDTSDATV
                     ESGLFRAEGTRGTCRVVCFSPRHDLTIAGMATSEIRRIVDVWADETTELGRRYPWVQV
                     FENRGEAMGASNPHPHGQIWAGAARPVEAMLEDREQAAHFEATGRPLLLDYARQERGG
                     PRVVADCPGWLVVVPFWAAWPFETLLIPTTRAARLADLDNAARDGLAAALQDLTRRYD
                     ALFDRPFPYSMGWHQAPFDAASTEPWQVHAHFYPPLLRASVRKFMVGYELLAEPQRDL
                     TPEEAAARLRAAVPASAD"
     gene            5981..>7077
                     /gene="argH"
                     /locus_tag="AC162A_08520"
     CDS             5981..>7077
                     /gene="argH"
                     /locus_tag="AC162A_08520"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR5010924.1"
                     /translation="MRMWGGRFDDEPDPRMADFTRSIEVDAELALDDIAGSIAHVRGL
                     GRAGLLTGDEVATLEGGLAALRHDVEAGAIAWDPVLEDVHLNLETLLTARVGAAGGKL
                     HTGRSRNDQVATDLRLWTRRAIDRLDAAIVGLERALVDLAERDGDAVLPGSTHIQPAQ
                     PVLFAHHLLAYVEMLERDRGRLADARTRVNVSPLGAGALAGAGYPLDRETTAAELGFD
                     GVSANSLDAVSDRDFVVEVLAAAALAMVHLSRLAEEITWWSNPRFGFVRVADAFSTGS
                     SMMPNKKNPDPAELVRGRTAAVIGAVTTMLALLKGLPLAYQRDLQEDKPALFGAVATL
                     EASLGVMAGLVETLAIDRVRMREAADEGYTTA"
BASE COUNT         1085 a         2337 c         2550 g         1105 t
ORIGIN      
        1 gtcgggttct cctcgggcgt ctcgggcggg cgatggggga acggcatctg gacgttggtg
       61 tagatcgggt gatcccacgt gtcctgcatc gtccagcagc cggggacgtc ggcctcgccc
      121 cagtcggccc ccggagcctc gtccggccgg tggagcagct ggaaccgcca gcgaccgtcg
      181 agggggaggc gatcgtcgtg ggtgaccgcg tgcatcggca gccgcccgcg ggacgtcagc
      241 tctgggtcgg cccacggcgc gggcgtcatg cggtgcggtc ctcccaatgt gccggtcccg
      301 gcaggccgga tcagcccttg gtcgccccga gcgacaggcc actgatgaag tgcttctgca
      361 gggccaggaa cacgaggatc gtcgggatcg cggcgagcag ggcccccgcc gcgagcaggt
      421 tggtatcggt gaagaactgg ccctggaggt tgttcagcgc cgacgtgatg ggtcgccggt
      481 ccccgtcctt gatcaggatc agcgcccaaa agaagtcgtt gtagatgaac gtgaacaaca
      541 gggtcgcgag ggcggcgagc gcgggccggc agagcggcat gatcacgccg ccccagatcc
      601 gcaggacgct cgcgccgtcg accagggcgg cttcggtcag ctcatgcgag atcgtcttca
      661 tgtagttgct cagcacgaat gtgcagaagc cgagctggaa cacgaagttg atgaggaaga
      721 ttccgaagta ctggttgtac aggagcgcgt tatcgctcag cggcgggggg atgcctcgca
      781 gggcggtgta gagcttgaac agcgggacga tgatcacctg gggcggcagg aggttgccgg
      841 ccgtgaagat cagcagaatc acgaggttga agcgccagct gaagcgcgac accgagaacg
      901 ccaccatcga cgcgacgatc agcgtcacga tgacggccgg gatcgtcacg atcagggtgc
      961 tcaggaagtg ctgggcaaag tcgccgccgt tccaggcgtc gatgaagttc tggaagttca
     1021 gggtctgggg cagcgagatg tagccggccg cctgggtgtc cgaatacggc cgcagggcgg
     1081 tgtagatggc ccatgccagc gggaacagcc agacgattgc catgagcgag aggaacacgt
     1141 acatcgcgat ccggccccac ggggtcgggc gccgcttccg gggcacggct gcgtcggccg
     1201 ccagcagctc gggcgtcacg accgtcgtca tcgctgcccc tggtcgcgca tcatccgggt
     1261 caggaacgcc acgatcggcc ccagcgagat gaccagcagc acgacggcga tggccgaccc
     1321 gaatccgagc cggttggact cgctgatggc gttgttggtg atcagcgttg acaacagctc
     1381 gagcccgttc ttgccgcggt tgatgatgta ggcgatgtcg aacgctcgca gcgcttcgat
     1441 cgtcgtgatc accacgatga cgacgttgat cggcatcatc accgggaaca cgacccggaa
     1501 gaacgtctgg cgctcgttgg ccccgtcgac cttggcggcc tcgcgcagcg tcgggtcgaa
     1561 cgccttcagg cccgccaggt agaggaccat gatgtagccg acgtggcgcc agctcgtggc
     1621 gacgagcgcg gcccacaggt tgaggctcgg gtcgcccagc cagtcgatca ggttgttgtt
     1681 ggcggtccgg ccgagcacgt tgttgatgaa cccctgctcg ggcgcgtact gcagcttcca
     1741 gatgatcccg atgacagcca gggacaggac gaccggcagg tagaagaccg tctggtagaa
     1801 gcgcgtgccg cgcccgacgc ggtcgaggag caccgcgaac aggatcccga tgggggtggc
     1861 gatgaacatc agccacgcga gccagatcag gttgtgggcg agcgccggcc agaagaacgg
     1921 atatgcggtg aacaggttct ggtagttggt gatcccgatg accttggcgt cgaacggact
     1981 gccgatcccg ttccagttgg tgaacgacag gaagaacgag acgatcgtgg ggccccagat
     2041 gaagatcagg tcaagggcca gcgggatccc gaccatcaat ccgaggacga gcttgtcccg
     2101 cccggtcagc aggctgtagc gccgccggaa gcggccggtc gtgacgtgct gcgctcccgg
     2161 ctgtgcgatg ggggagacag cagacgcccc cgtcacgccg ctttgatcgg cggtggcggg
     2221 ggcgacaggc tggctcgagc gttcggccac ggtccggaga gtctagccag accgattgaa
     2281 tggggctaca gggtgtccca gaagctctgg atcttcgcga ggaaggcgtc cagatcctgg
     2341 ttcgggttct tgaggaagtc gagcaggaac gcctgcatgc cgttcgggcc cgcgaagttc
     2401 gggttcgtgt cgcggtccag gaactgggtg atccgctggg cgccaccgat gagctccgcc
     2461 gccttcttct ggaggtcggt gtagccgctc gtgtcggcgt ccttggcggc cgcgatgttg
     2521 cccttgtcct gcgagaccca ggccacctgg gtcgccggct gggcgaggta ctccatgaac
     2581 gccttggagg catccaggtc ggccgccagc gtcggcgagt tggcgctgat catgaagccg
     2641 tcgatcggcg cgtcgagcgc cttctcggcg tcgtactcgg tcccgaagtt cgggtagggg
     2701 aagaagtcga ggtccgcgag gtcggcgtcg ccggcctcct ggaactgctg ggacacgaac
     2761 atcccgagca gatacatgcc ggccttcttc tggaccagcg cctgcgcggc gtcctgccag
     2821 gtccggccgg cggcaccctc ctggtagaac gggaggagcg tcttccaggt ctcgaaggcc
     2881 gccttggtct tcggatcggt ccacttctgc tcgccggcca tcagcccgac gtggaagtcg
     2941 tacccgttct gccgcaggtt gatgatgtcg aacgtgccca tcgccggcca gccgtcgctg
     3001 tcgccgaatg ccatcgggat gaggccgtcg gtctgcatct tcgtggccag cgccttgaac
     3061 tcgtcgagcg tcgtcgggat ggtgtatccc ttgtccgcga acatgctctt gcggtagaag
     3121 acggcccacg ggtagttgta gatcgggatg aagtactgct tgccgtcgtt cccggtggag
     3181 cccaccttga acgcatccga gtagttggcc ccgatcttgg cccagacgtc gctgatgtcg
     3241 gtcgcgaggc cctgctcggc gaagaaccgc atccggaagc ccgagaacca ggtgaacacg
     3301 tcgtccggcg tgccctggag gtaggagctg atctggtcct ggaaggtgcc gtggtccgtg
     3361 gtgttgatct tgaccgggat cccggtcgtc gtggtgaacg tgtccacgat cccggcgagg
     3421 acgcccttgg gaatcgcgtc cgagtagttc gatccgagcg tgacgctgcc gctggcaccg
     3481 gtcgacgggg cttcgacaga cgcagcctca gtcgccgccg gcgtggccgc cggtggctgt
     3541 gtggccgcgg ccgacggggc cgagcttgcg gcgggcgtgc tgcaggcggc gaggaaggcg
     3601 gacacaccgc cgagggccgc aacggcggcc ccacccttca gcacctggcg gcgcgtgacg
     3661 gatcggaggc cgtcgaagtc atccatacga tctcctcctg ggtggaccga cccgcgacgt
     3721 atcggcggag taggccacag tccgaattgt tgcgacaggg tgccgcaatg tcaagagact
     3781 cgcgcgtatt cgaacgagtt tttttgtgcg acttcgttga cttcggcccg gttcggcttc
     3841 aagatgagcg ggcgccgacc catcggccgc agtcaggcgg tcaaggtggc agccgtcccg
     3901 gcgcggggaa ctgggagcgg agtggatgct ggcccgccaa cgccaggcac tgatcctcga
     3961 ccgggtccgc gagagcggcg cggttcgcgg ggccgacctc gcgcgcgagc tcgaggtgtc
     4021 ggacatgacg gttcgtcgcg acctcgagat cctccacgac cacggcctgc tcgagaaggt
     4081 gcacggcggc gcgaccgcca tctccggcct cgcctcctac gagccggggt tcgtggcgaa
     4141 gtcggccctc cagcaggacg agaaggccgc gatcgccgcg gaggctgcgc gcctcgtcga
     4201 gccgggcatg gcgatcggga tctccgcggg cacgacgacg cacacgctcg cggcccgagt
     4261 cgcggacatc cccggcgtga cggtggtcac caactccctg cgcgtcgccg aggtcctgta
     4321 tcggtccggc cgccgcgacc agaccgtcat cctgaccggt ggggtgcgca cgccgtccga
     4381 ggcgctggtg ggctcattcg cggtcgcgca gctgcgatcg gtccacctcg acctcgtgtt
     4441 catgggcgtg cacggcatgg acggcaaggc cgggttcacc tgccccaacc tgtcggaggc
     4501 cgacacggac cgcgcgctca tcgaggccgg ccggcggctc gtggtcctgg cggatcacac
     4561 caagtggggc gtcatcggca tcgcctcgat cgcgcgcctg gaccaggccg acgtgctcgt
     4621 ctccgacacg ctgctcgacc cggcggccca gtcgacgctc cgggaggtcg tcggcgaagt
     4681 cgtcctggtc gacgcgggcg agccggccga tggcgggaac ccggtggggc tggacgatgc
     4741 cgatgagcgg ttgcaggccg cgggccgtgc ccactgagga gcagccccga ccttccgacg
     4801 gggcggtcca ggcgctcgcc gcggagccgc accgtcgcca caacgccctg accggcgagt
     4861 gggtcctggt gtcggcgggc cgcaccgacc gcccgtggct cggcgcggag gagcccgagc
     4921 cgcccgagga gcggcccaca tacgaccccg gctgctacct gtgcccgggc aaccggcggg
     4981 tgaaaggcga cgtgaacccg cactacgacc agacattcgt gttcaccaac gacttcgcgg
     5041 cgctccgccc cgacacgtcc gacgcgaccg tggaatcagg cctgttccgg gccgagggca
     5101 cgcgcgggac ctgcagggtc gtgtgcttct cgccccgcca cgacctgacc atcgccggga
     5161 tggcgacgag cgagatccgg cgcatcgtcg acgtctgggc ggacgagacg accgagctcg
     5221 ggcgtcgcta cccgtgggtg caggtgttcg agaaccgggg cgaggcgatg ggcgcctcga
     5281 acccgcatcc gcacggccag atctgggcag gcgcggcgcg gccggtcgag gcgatgctcg
     5341 aggaccgcga gcaggccgct cacttcgagg cgaccggccg gcccctgctg ctcgactatg
     5401 cccggcagga gcgcggcggg ccacgggtcg tggctgactg tcccggctgg ctggtggtgg
     5461 ttccgttctg ggccgcctgg ccgttcgaga cgctcctgat cccgacgacg cgcgccgccc
     5521 gcctcgcgga cctcgacaac gccgctcggg acgggctggc agctgccctg caggacctca
     5581 cgaggcgcta cgacgccctc tttgaccgac cgttcccgta ctcgatgggc tggcaccagg
     5641 cgccgttcga tgccgcgtcg accgagccgt ggcaggtcca cgcccacttc tacccaccgc
     5701 tgctgcgcgc gagcgtgcgc aagttcatgg tgggctatga gctgctcgcc gagccgcagc
     5761 gggacctgac gccggaggag gccgccgctc ggctccgggc ggccgtcccc gcgtcggccg
     5821 actgacgctc gtcgcccctg cacccgctcg gggtgatgac cctccaggca tcagatgcgc
     5881 agggtgtcat cgggctcggg tgacctcttt cgcggcgcgg tagggtcgct gtcgggcacc
     5941 agcgccagta cgatggcgcc acctgggcag cggagggcgc atgcggatgt ggggcggccg
     6001 gttcgatgac gaaccggacc cgcggatggc ggacttcacc cgatcgatcg aggtcgacgc
     6061 cgagctggcg ctcgacgaca tcgcggggtc gatcgcgcat gttcgcggac tcggccgcgc
     6121 gggcctgctg accggcgacg aggtcgcgac gctcgagggc ggtctcgccg cgctgcggca
     6181 cgacgtcgag gcgggcgcga tcgcctggga tccggtcctc gaggacgtcc acctcaacct
     6241 cgagacgctc ctaaccgccc gggtcggcgc cgcgggcggc aagcttcaca ccggccggtc
     6301 gcggaacgac caggtggcca ccgacctgcg cctgtggacc cgccgtgcga tcgaccgtct
     6361 cgacgccgcg atcgtcgggc tcgagcgggc gctggtcgac ctcgccgagc gcgacggcga
     6421 cgccgtgctc ccgggttcga cgcacatcca gcccgcccag cccgtcctgt tcgcccacca
     6481 cctgctcgcc tacgtcgaga tgctggagcg ggatcgcggc cgcctcgccg atgcacgcac
     6541 ccgggtcaac gtctcgccgc tcggggccgg cgcccttgcc ggcgcggggt atcccctgga
     6601 ccgtgagacc acggcggcag agctcggctt cgacggggtc agcgcgaact ccctcgacgc
     6661 cgtgtcggac cgcgacttcg tggtggaggt gctcgccgcg gcggccctcg cgatggtcca
     6721 tctcagccgc ctcgcggagg agatcacctg gtggtcgaac ccgcggttcg ggttcgtgcg
     6781 cgtcgccgac gcgttctcga cgggatcgtc gatgatgccc aacaagaaga accccgaccc
     6841 ggcggagctc gtccgtggcc gcaccgccgc ggtgatcggg gccgtgacca cgatgctggc
     6901 cctgctcaag ggcctcccgc tcgcgtacca gcgcgacctc caggaggaca agccggcgct
     6961 gttcggcgcc gtggccaccc tcgaggcgtc gttgggcgtc atggcgggcc tcgtcgagac
     7021 cctcgcgatc gatcgcgtgc gcatgcgcga ggcggccgac gagggctaca cgaccgc
//
LOCUS       DBNHXO010000421         3720 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3299208, whole genome shotgun sequence.
ACCESSION   DBNHXO010000421 DBNHXO010000000
VERSION     DBNHXO010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3299208"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="AC162A_08525"
     CDS             complement(<1..380)
                     /locus_tag="AC162A_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010925.1"
                     /translation="MPEPRPTGDRTPAQRRADHASLAQLSESLVPALVQKLSSSGLGE
                     LEVREGDWRIRLRRSNPAAASAPRRADRPRLGSHADREGRGRETTPTASGASQAQAGA
                     APAVPRRTMVTSPAVGVFRPDVKPG"
     gene            complement(384..1481)
                     /locus_tag="AC162A_08530"
                     /pseudo
     CDS             complement(384..1481)
                     /locus_tag="AC162A_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
     gene            complement(1402..2253)
                     /gene="accD"
                     /locus_tag="AC162A_08535"
     CDS             complement(1402..2253)
                     /gene="accD"
                     /locus_tag="AC162A_08535"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIR5010926.1"
                     /translation="MPRRFPTFGSRREAYPEAMWTRCPSCQEQLFNKQLEKAMSVCPS
                     CGHHFRLPAPVRISQLLDEDSFEERDAGLVSVDPLSFVDQKAYPDRIAAAQLATGLRD
                     AAVWGFGRIEAQRIAILVMDFGFMGGSMGAVVGEKVARAAEAALEERVPLVIVSASGG
                     ARMQEGTLALMQLAKTMAALERLRAARVPFVSVMSDPTTGGVFASYAAMGDVNLAEPN
                     ALIGFAGARVGAATIGQELPPGFQRAEFLYRHGFVDRVVQRPDLRRELAQVLRFLAPV
                     HAEATRG"
     gene            complement(2253..2822)
                     /locus_tag="AC162A_08540"
     CDS             complement(2253..2822)
                     /locus_tag="AC162A_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010927.1"
                     /translation="MTGGVPPENRSDVEALVADRYLEALLAAGDRGADDAPFDAGLDP
                     ETRRAVVLLRRALVRVHPSFRFEERLAGRLAELADARTAARGRVDGIVVAFPPPGRGT
                     RQDAADPLLPLILAGSLDPADDAVPDIDSRLAGARRPLLVGGAITSAALSLVGVAWVA
                     WRATRTAPRNVDPAAAGLDPVSGGLGGLS"
     gene            complement(2819..3448)
                     /locus_tag="AC162A_08545"
     CDS             complement(2819..3448)
                     /locus_tag="AC162A_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5010928.1"
                     /translation="MFGRREGAARGGVVPLDRDRSLVEAARRDPAQFDALYRKYLAQV
                     YAYALYELADHHAAEDATERVFMRALAALPRFSEQASRDDGPDASTFRVWLFRIARNV
                     VANERRTRRRHPSASLELALGAGLVVADPDDLAFGVATRDEAAQALRALDRLPDDRRR
                     ALVLRFVDEMTTSEIAGVLGRSEGAVRVLIHRGLNAVAKELGQDRGSRR"
     gene            complement(3465..>3720)
                     /locus_tag="AC162A_08550"
     CDS             complement(3465..>3720)
                     /locus_tag="AC162A_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010929.1"
                     /translation="AAFTERLEGRVEALRGGAFDIATWAGRQATTGRWVTIDDDPVAV
                     RALGVDAGSGGLLVVDAGDPTGERLVHAGEVRRVRLAPAV"
BASE COUNT          517 a         1386 c         1370 g          447 t
ORIGIN      
        1 ccgggcttga cgtccggccg gaagaccccg accgcgggcg aggtcaccat ggtccggcgc
       61 ggcacggccg gcgccgctcc ggcctgcgcc tgcgaagcac ccgatgccgt cggtgtcgtc
      121 tcgcgaccac ggccctcgcg gtcggcgtgc gatccgaggc gcggccggtc cgcgcgccgg
      181 ggcgccgacg cggccgccgg gttcgaacgg cgcaggcgga tccgccagtc gccctcgcgg
      241 acctcgagct caccgagccc gctcgacgag agcttctgca cgagcgccgg caccagcgac
      301 tcggacagct gggcgaggct tgcgtgatcg gccctgcgct gagcgggggt tcggtcgccg
      361 gtcgggcgcg gttcgggcat gcgctagacg tcctcgcggg cgggtggctc gtcgcggcgg
      421 ccgacgggga gcggggccgg gatcgcccgc tggcccgctt cgaacaggct gcgcaggcgg
      481 tccgccaggc cgggccgcgg tgggatggtg ggcgttggcg ccggaccctc gatctcgtcg
      541 aatgcgccga gcgaacggta ccggtgatag cgcgcctcga ccagcgcatc gagcgggatg
      601 gccgacaggc ggtcgagctc acgcacgatc gccgacttga ggcgtcgggc cgtctccgcc
      661 gggtcggcct gggcgccgtc ggcgggctcg gggatcacct cgtcgatgac gcccagccgc
      721 tgctggtcga tcgcgctcat cttcatcgcc aaggccgccg tggcggcctc gtcggaggtc
      781 cgccacagga tgctcgcgca gccctcgggc gagatcacgg aatagaccgc gttctcgagc
      841 gccagcacca cgtcaccgac ggcgatcgcg agcgcgccgc cggagccgcc ttcccccgtg
      901 atgatggtga cgatcggcgt ccgcaagcgg gtcatcaggc cgacggaccg ggcgatcgcc
      961 tcggcgatcc cgcgctcctc ggattcgggg cccgggtggg cgcccggcac atcgacgaac
     1021 gtcacgacgg gcagcccaca gcgctcggcg agctccatga ggcgcatcgc cttgcggtag
     1081 ccctcggggt gcggcatccc gaagttgcgc cgcacgttct cctcggtgtc ggcgcccttc
     1141 tgctggccga tcacgacgat ccgccggccc tcgatgcggg cgaagccggc cacgatcgcc
     1201 tcgtcgtcgc cgaacagccg gtcgccgtga agctcgacga actcgtcggc gatctcggcg
     1261 atgaactcga ggatgcgcgg ccggcgcagg ttgcgcgcca gctggacccg cgcccagacg
     1321 atcgccttct cgtcgacggg cggcggaaca ggcgcgttct cgcggccctc ggcgctctcg
     1381 cgcgtgcgcc cgcggatccg gtcaaccacg ggtggcctcc gcatggacag gtgcgaggaa
     1441 gcgcagcacc tgggccagct cgcgccgcag gtccgggcgc tggacgaccc ggtccacgaa
     1501 gccgtggcgg tacaggaact ccgcccgctg gaagcccggt ggcagctcct ggccgatcgt
     1561 cgcggcgccc acgcgcgcgc cggcgaaccc gatcagggca ttgggctcgg ccaggttcac
     1621 gtcccccatc gccgcgtacg aggcgaacac cccgcccgtg gtcggatccg acatgaccga
     1681 cacgaacggg acacgcgccg cccgcagccg ctcgagggcg gccatcgtct tggccagctg
     1741 catcagcgcc agcgtaccct cctgcatgcg ggcgccgccg gacgccgaga caatcaccag
     1801 cggcacgcgc tcttcgagcg ccgcctcggc cgcgcgggcc accttctcgc ctaccaccgc
     1861 gcccatggac ccgcccatga acccgaagtc catcacgagg atcgcgatcc gctgggcctc
     1921 gattcgcccg aacccccaga ccgcggcgtc gcgcagccct gtcgcaagct gggcggcggc
     1981 gatgcgatcc ggataggcct tctggtcgac gaacgagagc gggtccaccg acaccaggcc
     2041 ggcgtcccgc tcctcgaacg agtcctcgtc gagcagttgc gagatgcgga ccggcgccgg
     2101 caggcggaag tggtggccgc acgacgggca cacgctcatc gccttctcga gctgcttgtt
     2161 gaacagctgc tcctggcacg acgggcagcg cgtccacatc gcctcgggat aggcctcgcg
     2221 gcgggagccg aaggtcggga agcggcgggg catcaggaga gacccccaag accgccggaa
     2281 accgggtcga gaccggcggc tgccgggtcg acgttgcgcg gggcggtcct ggtggcccgc
     2341 caagccaccc aggcgacgcc gacgagcgag agcgccgccg aggtgatcgc gccgccaacg
     2401 agcagcggcc tgcgggcgcc ggcaagccgg gagtcgatgt ccgggaccgc gtcgtcagcg
     2461 ggatcgagcg agcccgcgag gatcagcggc agcaacgggt cggcggcgtc ctgccgcgtg
     2521 ccgcgacccg gcggcgggaa ggcgacgacg atcccgtcaa cccggccgcg ggccgccgtg
     2581 cgagcgtcgg cgagctcggc cagccgcccg gccagccgct cctcgaaccg gaacgagggg
     2641 tggacgcgga cgagggcgcg ccgcaggagc acgaccgccc tgcgtgtctc ggggtcaagg
     2701 ccggcgtcga acggcgcgtc gtcggcaccg cgatcgcccg cggcgagcag cgcctcgagg
     2761 tagcggtctg cgacgagcgc ctcgacgtcg ctccgattct ccggcgggac gccgcccgtc
     2821 atcgacgcga gccccgatcc tgccccagct ccttcgcaac cgcgttcagg ccgcggtgga
     2881 tcagcacccg caccgccccc tcggaccggc cgagcacccc cgcgatctcg ctcgtcgtca
     2941 tctcgtccac gaaccgcagg acgagcgcgc ggcggcggtc gtcgggcaga cggtcgagcg
     3001 cccgcagggc ctgggcggcc tcgtcgcggg tcgcgacgcc gaacgcgagg tcgtcgggat
     3061 cggcgacgac aaggccggcg ccgagcgcga gctcgagcga ggccgaggga tggcggcgcc
     3121 gcgtgcgacg ctcgttcgcg accacgttgc gcgcgatccg gaacagccag acgcggaagg
     3181 tcgaggcgtc cgggccgtcg tcgcgggacg cctgctccga gaagcgcggc agcgcggcga
     3241 gggcgcgcat gaacacgcgc tccgtcgcgt cctcggcggc gtggtggtcg gcgagctcgt
     3301 agagagcgta ggcatacacc tgggcgaggt acttccgata cagggcgtcg aactgggcag
     3361 gatcgcgacg cgcggcctcg acgagcgagc ggtcgcggtc gagcgggacg acgcctcctc
     3421 gcgccgctcc ctcccgcctt ccaaacaccg gtcgcgccat cctgttacac cgccggcgcg
     3481 aggcggacgc ggcggacctc gccggcatgc acgagccgct cgccggtcgg gtcgcccgca
     3541 tcaacgacca ggagcccgcc cgagcccgcg tccacgccga gggcacggac agcgaccggg
     3601 tcgtcgtcga tcgtcaccca gcggccggtc gtggcctggc gcccggccca ggtggcgatg
     3661 tcgaaggcgc cgcctcgcag ggcttcgacc cggccttcga gccgctccgt gaacgccgcc
//
LOCUS       DBNHXO010000422         3960 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2506409, whole genome shotgun sequence.
ACCESSION   DBNHXO010000422 DBNHXO010000000
VERSION     DBNHXO010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2506409"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2014)
                     /locus_tag="AC162A_08555"
     CDS             complement(<1..2014)
                     /locus_tag="AC162A_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR5010930.1"
                     /translation="MTEPQQDRVSGPLTPIVPEWLANLAALGWRVFVIVALVVALWLL
                     ASELWTVTASITVAVVIAATFAPIVVRLRGRGRSRAAAAAIVWVVALVVLLLVGVVLG
                     LALIPYIADILREVEASITRLQADLAATNIPPEVVTVLRDLISAVRGGSGDIMSALGA
                     QVASAVTIMVLAVFLIFFFLKDGDKAWVWIFQAASDQKRERITDAGDDALWRVGGYLR
                     GTTILSAIIAITDYVFMLVLGVPLAAPLTLLVFLSGYIPYFGGIVTTALILLVTYASL
                     GVGPVIAMLVLIGIRNVILGYGIRPAIYGRTVSIHPALVLVALPAGFELAGVVGLFAA
                     VPVAAIILAVANATVSILDPGPRPELPALVPGWIDRVAQWSWRLLVAAGLVGLFVGIF
                     VAMPLVVIPALLATILAATLDPLVSALKQRGWSRGKAAAVATGGGFLAVVGILVVAMI
                     SLVGQAGDIQTATASGASSADASTGGQLGLLAGAVTSGGADAVRAVAQTASAIGQIAV
                     IVILSTLLAFYFLKDGVDLWAKILGRVRADVAPEVNAAATRAFDVLGGYMIGTGAISF
                     VGAASQFVIMVVLGLPLALPVFVLSFILCFIPYIGGFISTGIAFLITISVGSPTDIAI
                     MAVWTVVFNIVTGNIVTPLVYGRTVHLHPAVVLVAIPAGAAIAGILG"
     gene            2191..3846
                     /locus_tag="AC162A_08560"
     CDS             2191..3846
                     /locus_tag="AC162A_08560"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR5010931.1"
                     /translation="MRDLLAPVLRSIRVSAFAMRNGTIRRAMVAYLAFNTVEMASWTT
                     ILVYAYGATGPASVGLVAILQLIPSAVAAPLLANIGDRFPRATVLLGWFLAQAFLLAA
                     TGVAILVGAPPLVVYLVSAVASISLTQTRPILASLLPELSRSPEELTAANAWSTIFIG
                     IGGFLGPIGAAVLLAVADAEVTFLAAAGVLAAAAVLLIGLRGPVEAPLASPDDAGAPA
                     DGGLRSIAAGLREVARDRDLLVVNALLTGEYVIYGGLEILLVLLAIELLGIGESGAAA
                     LVGAVGLGGIIGGAAAFALVGRRRLAPWLGVAAVVIGLQIALLGVDPAARTAALLVVV
                     SGIGFAIIDTAGQTLLQRITPDVVRARVFGVLEGLLLVGEAIGSVIVPPLASVFGLQG
                     TAIAIGLLLPLLALLAVGRFASIDARVKLPEAELRALRQVPMLAPLGPATIETLARHL
                     VRVPVVTGSVVIREGDVGDRWYLVERGAFDVTVAGEFRPSLGPGDAFGEIALLRDVPR
                     TATVTAASDGLLWALERDEFLAGVTGSPSALREAERVAATRLS"
BASE COUNT          581 a         1402 c         1421 g          556 t
ORIGIN      
        1 tgcccaggat gcccgcgatc gcggcccccg ccgggatcgc gacaagcacg acggcggggt
       61 gcaggtggac cgtccgcccg tataccagcg gcgtgacgat gttgccggtg acgatgttga
      121 acacgacggt ccagacggcc atgatcgcga tgtcggtcgg ggagccgacc gagatcgtga
      181 tgaggaatgc gatgccggtc gagatgaagc cgccgatgta ggggatgaag cacaggatga
      241 atgacaggac gaacaccggc agcgccagcg ggagcccgag caccaccatg atcacgaact
      301 ggctggccgc tccaacgaag gagattgccc cggtcccgat catgtagccg ccgagcacgt
      361 cgaacgcccg ggtggctgcc gcgttgacct cgggcgcgac gtcggcacgc acgcgaccga
      421 ggatcttcgc ccacaggtcg acgccatcct tgaggaagta gaacgcgagc agcgtgctga
      481 ggatcacgat cacggcgatc tggccgatcg cggatgccgt ctgggccacc gccctcaccg
      541 cgtccgcccc acccgacgtg acggccccgg ccaggaggcc cagctggccg cccgtggagg
      601 cgtcggcgga cgatgcaccg gacgccgtcg ccgtctggat gtcgccggcc tggccgacga
      661 gcgagatcat cgcgacgacc aggatgccga cgaccgcgag gaacccgccg ccggtcgcga
      721 ccgcggcggc cttgccccgg gaccagcccc gctgcttcaa ggcgctcacg agcgggtcga
      781 gcgtcgccgc gaggatcgtc gccagcagcg ccgggatcac taccagcggc atggcaacga
      841 agatgcccac gaacagacct accaggcccg cggcaacgag gagccgccag ctccactggg
      901 ccacccggtc gatccagccc gggacgagcg cgggcagctc ggggcggggc ccggggtcga
      961 ggatcgagac cgtcgcgttg gcgacggcga ggatgatcgc cgccacaggt accgccgcaa
     1021 acaggccgac gacgcccgcg agctcgaagc cggccgggag tgcgaccagc acgagcgccg
     1081 gatggatgct caccgtgcgg ccgtagatgg cgggccggat gccgtagccc aggatgacgt
     1141 tccggatgcc gatcagcacg agcatcgcga tcacggggcc cacgccgagt gacgcatagg
     1201 tgacgagcag gatcagcgcg gtcgtcacga tcccgccgaa gtaggggatg tagccgctca
     1261 ggaagacgag gagggtgagt ggcgcggcga gcgggacacc caacacgagc atgaacacgt
     1321 agtcggtgat cgcgatgatc gcggacagga tcgtggtgcc gcgcaggtag cctccgacgc
     1381 gccacagggc atcgtcgccg gcgtcggtga tgcgctcgcg cttctggtcg ctcgcggcct
     1441 ggaagatcca gacccacgcc ttgtcgccgt ccttgaggaa gaagaagatc aggaataccg
     1501 cgagcaccat gatcgtgacc gcgctcgcga cctgcgcccc gagcgccgac atgatgtccc
     1561 cgctcccgcc gcgcaccgcg gagatgaggt ctcgcagaac cgtgacaacc tccggcggga
     1621 tgttcgtcgc ggccaggtct gcctgcagcc gggtgatgct cgcttcgacc tcgcgcagga
     1681 tgtcggcgat gtaggggatg agggccagtc cgagcaccac gccgacgagc aggaggacga
     1741 cgagcgcaac gacccacacg atcgccgcgg ctgcggccct cgaccggcct cggccgcgga
     1801 ggcgcacgac gatcggggcg aacgtggcgg cgatcacgac cgcgacggtg atcgacgcgg
     1861 tcacggtcca cagctccgag gcgagcagcc acagggcgac aacgagggcg acgatgacga
     1921 acacgcgcca gccgagggcg gccaggttcg caagccactc ggggacgatc ggtgtgaggg
     1981 gaccggaaac gcggtcctgt tgcggttcag tcatcggcgt cgtggccact cccggctacc
     2041 ggcgctgcgg gccggctcgg acccgggctg ccgcaggcgg tccactgtaa cgctgcggcg
     2101 acggcccggg ccgcgtatcc gcgcctccct gatcgagcct ccgacacgaa tcggctgcag
     2161 gagccccgcg acacgcccga tgctgggggc atgcgcgacc tcctggcccc cgtcctgcgc
     2221 tcgatccggg tctcggcgtt cgcgatgcgc aacggcacca tccggcgcgc gatggtcgcc
     2281 tacctcgcgt tcaacaccgt cgagatggcc agctggacga cgatcctggt gtacgcctac
     2341 ggggcgacag ggcccgcgtc ggtcggcctg gtggccatcc tgcagctcat tcccagtgcc
     2401 gtggccgccc cgctgctcgc gaacatcggc gaccgtttcc cgcgcgcgac cgtgctgctc
     2461 ggctggttcc tcgcccaggc cttcctgctc gctgcaaccg gtgtcgccat cctggtcggc
     2521 gccccgcccc tggtcgtcta tctcgtgtcg gcggtcgcct cgatctcgct gacccagacc
     2581 cggccgatcc tggcgtcgct cctccccgag ctctcccgaa gcccggagga gctcaccgcg
     2641 gccaacgcct ggtcgacgat cttcatcggc atcggcggct tcctcgggcc gatcggggcg
     2701 gccgtcctgc tcgcggtggc cgacgcggag gtcacgttcc tcgccgccgc gggcgtgctc
     2761 gcggccgctg ccgtcctcct gatcgggctc cgcgggccgg tggaggcgcc actggcgtcc
     2821 cccgacgacg ccggtgcacc ggcggacggt ggcctgcgct cgatcgccgc cgggctgcgc
     2881 gaggttgccc gggaccggga cctgctcgtc gtcaacgccc tgctgaccgg cgagtacgtg
     2941 atctacggtg gtctcgagat cctgctggtg ctgctcgcca tcgagctcct tggcatcggc
     3001 gagtcgggcg ccgccgccct cgtcggcgcg gtcggcctgg gcgggatcat cggtggcgcg
     3061 gcagccttcg ccctcgtcgg gcggcggcgc ctcgcgccgt ggctgggcgt cgcggccgtg
     3121 gtcatcggcc tgcagatcgc tctgctgggg gtcgaccccg ccgcccggac cgcggcgctc
     3181 ctggtggtgg tctcggggat cggcttcgcg atcatcgaca cggcgggcca gaccctgctc
     3241 cagcgcatca cgccggacgt cgttcgcgcc cgcgtcttcg gcgtgctgga aggactgctc
     3301 cttgtcggcg aggccattgg ttcggtgatc gtgccgccac tcgcgtcggt gttcggcctc
     3361 cagggcacgg cgatcgcgat cgggctgctc ctgccgctgc tcgcgctgct ggccgtcggg
     3421 cggttcgcca gcatcgacgc gcgcgtgaag ctgcccgagg cggagctgcg tgcgctgcgc
     3481 caggtgccga tgctcgcccc gctcggaccc gcgaccatcg agacgctggc ccgccacctc
     3541 gtccgcgtgc ccgtcgtgac cgggtcggtc gtgatccggg agggagatgt cggcgaccgc
     3601 tggtatctcg tcgaacgggg cgcgttcgac gtgacggtcg ccggcgagtt ccgacccagc
     3661 ctcgggccgg gtgacgcgtt cggcgagatc gcgctgctcc gcgacgtccc ccggacggcg
     3721 acggtcacag cggccagcga cggcctgttg tgggccctcg agcgcgacga gttcctcgcc
     3781 ggcgtgacgg gctcgccgag cgcgctgcgc gaggcggagc gggtggcggc gacacgcctc
     3841 agctgatacc tcaggtcgga ggctcgggct cgcccatcgt cacgaccaga ggtccgtgct
     3901 caggtacttc atgcccgagt ccgcgagcag gatgacgacc gtcccgcccg cgagtggacc
//
LOCUS       DBNHXO010000423         2694 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_722122, whole genome shotgun sequence.
ACCESSION   DBNHXO010000423 DBNHXO010000000
VERSION     DBNHXO010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_722122"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="AC162A_08565"
     CDS             <1..708
                     /locus_tag="AC162A_08565"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR5010932.1"
                     /translation="PHTLYGHAPMFEWQVLAGAGVSVLASNPRGSEGYGEAFNRANLG
                     DWGDGPMADVLAGVDQAIEDGLADPERLGVTGGSYGGYLTNWIVGKTERFKAAVTCRS
                     VVDMRLLFLTGDISSGEWARTEFGRNPWEDPTYFDSISPLSLAVNVRTPLLIQHSERD
                     LRTTVGQAEALFTVLRSLKRPVRFMRVPDESHELTRGGAPYRRAENLVQVRDWFVHFL
                     VRGERRLPPPPRNRAGR"
     gene            719..1699
                     /gene="deoC"
                     /locus_tag="AC162A_08570"
     CDS             719..1699
                     /gene="deoC"
                     /locus_tag="AC162A_08570"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR5010933.1"
                     /translation="MTTTVVRDAAARERANELIRAWDSRAGSLGSLLGRVTPVDAVGI
                     NERVTSLTRRSIKKASKVWALDLAIRMMDLTTLEGKDTPGKIRALCAKGMRPQPGDAS
                     IPHVAAICVYPTFVAEAKEALGSSGVKVASVSTAFPSGQSFLDIKLAETRAAVAAGAD
                     EIDMVIDRGAFLAGDYATVFEEIVAVREACGETHLKVILETGELVTFDNVRRASVLAM
                     AAGADFIKTSTGKVTPAATLPVTLVMLEAIRDFERVTGKSIGMKPAGGIRTAKEAIGY
                     LVVLYETLGPRWMTPDRFRFGASSLLNDVLMQLEKERTGAYQRPDHFTID"
     gene            complement(1713..>2694)
                     /locus_tag="AC162A_08575"
     CDS             complement(1713..>2694)
                     /locus_tag="AC162A_08575"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR5010934.1"
                     /translation="LETEPGSITGAAVRPDGEVWYRVHNGLHPATLLAIGSATPLLEP
                     RGAAAPAGRVFEPWWFENPHGQRVHGFLVRPDGEGPYPVMMRVHGGPQSLDMDRWVPD
                     LQAHVDAGFLVAMVNYRGSDGFGQAWRDELTGNVGFLELEDELAGLDDLVARGLADPA
                     RVVLAGWSWGGYVALLGLGRHPERWISGIAGVPIADFVASYEDEAPILQSLDRALFGG
                     SPETAPELFRERSPITYVDAVAAPVLIMAGENDSRCPIRQVWNYVGRLRARGVEPEVY
                     TYATGHASFDVEERVRQTAITLDFLARTVPGIERLEDIDAHVAAAGFAAA"
BASE COUNT          404 a          981 c          940 g          369 t
ORIGIN      
        1 ccgcacacgc tctacggcca cgcgccgatg ttcgagtggc aggtcctcgc gggggccggc
       61 gtgtccgtgc tggcgtcgaa cccccgcggc tctgagggct acggcgaggc gttcaaccgc
      121 gccaacctgg gcgactgggg cgatggcccg atggccgacg tgctcgcggg cgtcgaccag
      181 gcaatcgagg acgggctcgc cgatccggag cgcctcggcg tcaccggcgg ctcgtacggc
      241 gggtatctca ccaactggat cgtgggcaag acggagcgct tcaaggcggc cgtcacgtgc
      301 cgctcggtcg tcgacatgcg gttgctgttc ctgaccggcg acatctcgag cggcgagtgg
      361 gcgcgcaccg agttcgggcg caacccgtgg gaggacccga cctacttcga ctcgatctcg
      421 ccgctctcgc tcgccgtgaa tgtgcggacg ccgctgctta tccagcactc cgagcgcgac
      481 ctgcggacga cggtcggcca ggccgaggcg ctgttcacgg tcctgcgctc gctcaagcgg
      541 cccgtgcggt tcatgcgcgt ccccgacgag agccatgagc tcacccgcgg cggcgccccg
      601 taccggcgcg cggagaacct ggtccaggtc cgcgactggt tcgtccactt cctggtccgc
      661 ggcgagcggc gcctgccgcc cccgcccaga aaccgggcag gccgttagac tcgcgaccat
      721 gaccacgacc gtcgttcgtg atgccgcagc gcgcgagcgc gcgaacgagc tgatccgcgc
      781 ctgggacagc cgcgccggga gcctcggcag cctgctcggg cgggtgacgc ctgtcgatgc
      841 cgtcgggatc aacgagcggg tcacatcgct gacccgtcgc tcgatcaaga aggcctccaa
      901 ggtctgggcc ctcgacctcg cgatccggat gatggatctc acgacgctcg agggaaagga
      961 cacgcccggc aagatccgtg cgctgtgcgc gaagggcatg cgcccgcagc cgggcgacgc
     1021 gtcgatcccg cacgtcgccg cgatctgcgt ctacccgacg ttcgtcgccg aggcgaagga
     1081 agcgctcggc tcatcgggcg taaaggtcgc ttcggtgtcg acggccttcc cgtcgggcca
     1141 gtcgttcctc gacatcaagc tcgccgagac gcgcgcggcc gtcgccgccg gcgccgacga
     1201 gatcgacatg gtcatcgacc gcggcgcgtt cctcgccggc gactacgcga cagtgttcga
     1261 ggagatcgtc gcggtccgcg aggcctgcgg cgagacgcac ctcaaggtca tcctcgagac
     1321 cggcgagctc gtgaccttcg acaacgtccg gcgggcgtcc gtcctcgcga tggccgccgg
     1381 cgccgacttc atcaagacct cgaccggcaa ggtcaccccc gccgcgacgc tcccggtgac
     1441 cctcgtcatg ctcgaggcga tccgcgactt cgagcgcgtg accggcaagt ccatcggcat
     1501 gaagcccgcg ggcggcatcc ggaccgcgaa ggaagccatc gggtacctcg tcgtcctgta
     1561 cgagacgctg gggccgcgct ggatgacgcc cgacaggttc cggttcgggg cgtcgtccct
     1621 gctcaacgac gtgctgatgc agctcgagaa ggagcggacg ggcgcctacc agcgccccga
     1681 ccacttcacg atcgactgag gcgaggcccc gctcaggcgg ccgcgaagcc cgcggcggcg
     1741 acgtgggcgt cgatgtcctc gagccgctcg atgcccggca ccgtgcgcgc gaggaagtcg
     1801 agcgtgatcg ccgtctggcg gacccgctcc tccacgtcga acgaggcatg cccggtcgcg
     1861 tacgtgtaga cctcgggctc cacgccgcgg gcccgcaggc gccccacgta gttccacacc
     1921 tggcggatcg ggcaccgcga gtcgttctcg ccggccatga tcagcaccgg cgccgcgacg
     1981 gcgtcgacgt aggtgatcgg gctgcgttcg cggaacagct cgggcgcggt ctccggcgag
     2041 ccgccgaaca gggcacgatc gagtgactgg aggatcggcg cctcgtcctc gtaggaggcg
     2101 acgaagtcgg cgatcgggac gcccgcgatc ccgctgatcc agcgctcggg atggcggccc
     2161 aagcccagca gcgcgacata accaccccac gaccagccgg ccaggacgac ccgcgccgga
     2221 tcggcgaggc ctcgggcgac caggtcgtcc agcccggcga gctcgtcctc gagctcgagg
     2281 aagccgacat tgccggtcag ctcgtcgcgc cacgcctggc cgaacccgtc cgagccgcgg
     2341 tagttgacca tcgccacgag gaacccggcg tcgacgtgcg cctggagatc cggcacccag
     2401 cgatccatgt ccagcgactg gggcccgccg tgcacccgca tcatcacggg gtaggggccc
     2461 tcgccgtcgg ggcggacgag gaagccgtgc acccgctgcc catgcgggtt ctcgaaccac
     2521 cacggctcga agacccgtcc ggccggcgcg gcggccccgc gcggctcgag gaggggcgtc
     2581 gccgagccga tggcgagcag cgtcgccgga tgcagcccgt tgtggacgcg ataccagacc
     2641 tcgccgtcgg gccgtaccgc cgcacccgtg atcgagcccg gctccgtctc gagg
//
LOCUS       DBNHXO010000424         2010 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4094474, whole genome shotgun sequence.
ACCESSION   DBNHXO010000424 DBNHXO010000000
VERSION     DBNHXO010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4094474"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="AC162A_08580"
     CDS             complement(<1..554)
                     /locus_tag="AC162A_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5010935.1"
                     /translation="MNANDSTGTDAPGDGRRRVLVADDSESYRRVTRGQLVARGFDVV
                     AVGSGTEALARLATEPFDLLLVDGMMPGLDGPATAREIRRREAVEGEPRLPIVAITAS
                     GQPEDRERMLAAGIDDLVVKPVLDEDLDRALTRWLPSAGPRRETLIPARTEDDAWPEP
                     EPEPRGDGPVIDEAAFARLAVLGDA"
     gene            719..>2010
                     /locus_tag="AC162A_08585"
     CDS             719..>2010
                     /locus_tag="AC162A_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HIR5010936.1"
                     /translation="MTLTSSAAERDVSPLDPDELHLIDAYWRAANYLSVGQIYLLDNP
                     LLREPLLPEHVKPRLLGHWGTTPGLNLIYAHMNRVIRARDLDAIYVIGPGHGGPAIVA
                     NAWLEGTYSEVYPSITRDAEGMRRLFRQFSFPGGIPSHVAPETPGSIHEGGELGYALS
                     HAFGAAFDNPDLLVCCVVGDGEAETGPLATSWHSNKFLDPVRDGAVLPILHLNGYKIA
                     NPTVLARIPAGELNALMEGYGYRPYLVEGDDPADVHQQLAAALDAATAEIAAIQRAAR
                     EGGATERPRWPMIVFRTPKGWTGPAQVDGHQVEGTWRAHQVPMGEVRTNADHRALLET
                     WLRSYRPEELFDEHGSLRAELAALAPTGTRRMSANPVANGGELLRDLDLPDFRAYAVD
                     VPAPGATIAEATRVLGTWLRDVMDRNGETFRIFGPDETA"
BASE COUNT          286 a          781 c          680 g          263 t
ORIGIN      
        1 gcatccccga ggaccgcgag ccgcgcgaac gccgcctcgt cgatcaccgg gccgtcgccg
       61 cggggctcgg gctcgggttc gggccacgcg tcgtcttcgg tccgggccgg gatcagcgtc
      121 tcgcgacgag ggcccgcgga cggcagccag cgcgtcagcg cgcgatcgag gtcctcgtcg
      181 aggacgggct tcacgaccag gtcgtcgatc ccggccgcca gcatccgctc ccgatcctcc
      241 ggctgcccgc tcgcggtgat ggcgacgatc gggaggcgcg gctccccttc gacggcctcg
      301 cgccggcgga tctcgcgggc ggtcgccgga ccgtcgagcc cgggcatcat gccgtcgacg
      361 agcagcaggt cgaatggttc ggtcgccaga cgggcgagcg cctcggtccc gctcccgacc
      421 gcgacgacat cgaaaccccg tgcgaccagc tggccacggg tgacccgccg gtagctctcg
      481 ctgtcgtccg ccaccaggac gcgccgtcgc ccgtcgccgg gcgcgtcggt tccggtgctg
      541 tcgttggcgt tcacgcaggc tccattctca gcggtccgcg gccggaccat ctgccctctc
      601 cacgcgggaa cgtagccgcc caccggtggg ccggccagtc cccagttgag ggggacccgc
      661 ttgggcacga ccgaggcgta ggctagccac acaccactac gtttcctgga gccgtgccat
      721 gacgctgacc tcgagcgccg ccgagcgcga cgtatcgccg ctcgaccccg acgagctcca
      781 cctgatcgac gcgtactggc gcgcggccaa ctacctctcc gtcgggcaga tctacctgct
      841 cgacaacccg ctgctccgcg aaccgctgct ccccgagcac gtcaagcccc gactgctcgg
      901 gcactggggc acgacgcccg gcctcaacct catctacgcg cacatgaacc gcgtcattcg
      961 ggcgcgcgac ctcgacgcga tctacgtcat cggccccggg cacggcggac ccgccatcgt
     1021 cgccaacgcc tggctcgagg gcacctacag cgaggtctac ccaagcatca cgcgcgacgc
     1081 cgagggcatg cgccggctgt tccgccagtt ctcgttcccg ggcggcatcc ccagccacgt
     1141 cgcgccggag acgccggggt cgatccacga gggaggcgag ctcggctacg cgctctcaca
     1201 tgcgttcggc gcggcgttcg acaaccccga cctgctcgtg tgctgcgtcg tcggcgacgg
     1261 cgaagccgag accgggccgc tggcgacgag ctggcactcg aacaagttcc tcgacccggt
     1321 gcgcgacggc gccgtcctgc cgatcctcca cctcaacggc tacaagatcg cgaaccccac
     1381 cgtcctcgcc cgcatcccag ccggcgagct caacgccctg atggagggct acggttaccg
     1441 gccgtacctc gtcgagggcg acgacccggc ggacgtccat cagcagctgg cggcggccct
     1501 cgacgcggca accgccgaga tcgcggccat ccagcgggcg gcccgggaag gcggcgccac
     1561 cgagcgtccg cgctggccga tgatcgtgtt ccggacgccc aagggctgga cggggccggc
     1621 gcaggtcgac ggccaccagg tcgagggcac gtggcgcgcg caccaggtgc cgatgggcga
     1681 ggtccgcacc aacgcggacc accgggcgct cctcgagacg tggctgcgct cgtaccgccc
     1741 agaggagctg ttcgacgagc acggctccct gcgtgcggag ctcgccgcgc tcgccccgac
     1801 cgggacgcgg cgcatgagcg cgaaccccgt cgccaacggc ggcgagctgc tgcgcgacct
     1861 cgacctcccg gacttccggg cctacgccgt cgacgtcccc gctcccggcg cgaccatcgc
     1921 cgaggcgacg cgcgtgctcg gtacgtggct gcgcgacgtc atggaccgca acggcgagac
     1981 attccggatc ttcgggccgg acgagacggc
//
LOCUS       DBNHXO010000425         4169 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4094603, whole genome shotgun sequence.
ACCESSION   DBNHXO010000425 DBNHXO010000000
VERSION     DBNHXO010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4094603"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..707)
                     /locus_tag="AC162A_08590"
     CDS             complement(186..707)
                     /locus_tag="AC162A_08590"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIR5010937.1"
                     /translation="MSAETLRAKIREIPDFPKPGILFYDITTVLKEPGAYKEAIDLML
                     EPYRDEHVDIVVGMESRGFIFAAPIAYQLDTGLVPVRKLGKLPAETLTVEYALEYGSN
                     TLEIHRDAISPGQKVLIVDDLLATGGTVRGTIELVERLRGEVVGLAFLVELEFLKGRE
                     RLDGRRVTSVIKY"
     gene            complement(786..2231)
                     /locus_tag="AC162A_08595"
     CDS             complement(786..2231)
                     /locus_tag="AC162A_08595"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5010938.1"
                     /translation="MTAPAAAAQPAIVVPARTAELFAPGLRGLTVGLIATITLVALES
                     LAIGTVLPLVGEELGQIELYGWVYSSFFLGSLLGIVVAGGALDRMALWKPFAVGLALF
                     AIGLLIGGLAPSMPILVGARFIQGLGGGAVGPTAYVAIGRTLPDRLQPRMFALLSTAW
                     VVPGVVGPSIAAIVGEFTTWRLVFLGLIPFLAVAGGLAVSALRRIPDAAPPEEHAAAA
                     ATIRRLPNALLVAAGAGLLIASLTAQDLRLIVVGGLIGLALLLPAFRRLTPPGTLSLA
                     AGVPAAVLLRGLMTFAFFSGDAYIPLLLQTWRGTPATLTGLVFTGTTIAWTVATWLQA
                     RRIEIWGPHRFVALGFACVAVGSLITIPAVLPGFPAELTILTWLLPGLGMGFMFSAVT
                     LVVLRGSAPAEQGAASSALQLSDILGTALGAGVAGAITAFGTRQGGDALGWALAAVFA
                     LSSIAAGLGVIASRRIRPIKHVRSTLGAAVD"
     gene            2355..3119
                     /locus_tag="AC162A_08600"
     CDS             2355..3119
                     /locus_tag="AC162A_08600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5010939.1"
                     /translation="MPDDIEYVGLMAEAWDALRGDTSGWEDRAWFREVIRQRGEPVLD
                     VGTGTGRLLLDFLAQGVDIDGIDNAPDMLERLHAKAAAAGLDVDGRVHLGRMQSLRLS
                     RRYGTVIVPSSSFQLLLEPDDAAEAMRRFFALLLPGGSLAMPFIVMDKAYEEHWTKEA
                     ALEDGSIVRRTSTATFDPTVGIEATDDLYEVLNGSGPVRIEHFVRPRATRAYTRDQAR
                     ALYETAGFVDLEWGADFTFEDREHAEIFTIVGRRPD"
     gene            complement(3314..3916)
                     /locus_tag="AC162A_08605"
     CDS             complement(3314..3916)
                     /locus_tag="AC162A_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018708116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR5010940.1"
                     /translation="MPTVVDQLGKAAARGDTRSVLELIEAGADVNERDEDGRTPVMTA
                     TRGNHVDTVRALIEAGADIDLRDDRLDNPFLYAGAEGLLDILRLAIDAGADPALTNRF
                     GGTALIPASERGHVEVVEELLTRTEVDIDHVNNLGWTALLEAIVLSNGDERHQQVVQL
                     LVDHGADVTIGDKDGVTPLEHARMRGFTQIERILAAAAEG"
BASE COUNT          663 a         1462 c         1469 g          575 t
ORIGIN      
        1 gcggccgact cctgctggag cagctgcgcg gcgccgatga ccgagctcat cgacgtcatc
       61 acgtcgcccg cgaactgccg gaagaaccgg cgacggttcg ggtcgggcgg ctggacgggc
      121 tggtcggtca cggtcgggtc gagttgttca cgggtcgagg cgtggatccc gcctgtgcgc
      181 cggggtcagt acttgatgac gctggtgacc cggcggccat ccagccgctc gcggcccttg
      241 aggaactcga gctccacgag gaacgcgagg ccgacgacct cgccccgcag gcgctcgacg
      301 agctcgatcg tgccccggac ggtcccgccc gtcgccagca ggtcgtcgac gatgagcacc
      361 ttctgtcccg ggctgatcgc gtcgcggtgg atctcgaggg tgttcgagcc gtactcgagg
      421 gcgtactcga cggtcagcgt ctccgccggc agcttgccca gcttccgtac aggtaccagc
      481 ccggtatcga gctggtacgc aatcggcgcg gcgaagatga acccgcgcga ctccatgccc
      541 accacgatgt cgacgtgctc gtcccggtag ggctcgagca tcaggtcgat cgcctccttg
      601 taggcccccg gctccttgag caccgtcgtg atgtcgtaga acaggatgcc gggcttcggg
      661 aagtcaggga tctcgcgaat cttcgcgcga agcgtctcgg cggacatcgg gttcggggac
      721 ctccggtggt ggtcaggtcc ggcggcggcg aagccctggg cgatgcggtt cgcgcgtccg
      781 gacggctagt ctaccgcagc gccgagcgtg ctccgcacgt gcttgatggg cctgattctg
      841 cgcgacgcga tgacccccag cccggcggcg atcgaggaca gcgcgaacac cgccgccagc
      901 gcccagccca gggcgtcgcc gccctgccgt gtgccgaacg ccgtgatcgc cccggcgacg
      961 cccgcgccca acgccgtccc gaggatgtcc gagagctgga gcgccgacga cgcggccccc
     1021 tgctcggccg gcgcggaccc ccgcagcacg acgagggtga ccgcagagaa catgaagccc
     1081 atgccgagtc ccggcagcag ccaggtgagg atcgtgagct cggccggaaa gcccggcagc
     1141 accgccggaa tcgtgatcag cgagccgacc gcgacgcagg cgaaccccag ggcgacgaac
     1201 cggtgcgggc cccagatctc gatccgccgc gcttggagcc atgtcgcgac ggtccaggcg
     1261 atcgtcgtcc cggtgaacac gagccccgtg agcgtggccg gcgtaccgcg ccaggtctgg
     1321 agcaggagcg ggatgtaggc gtcgccgctg aagaaggcga aggtcattaa cccgcgcaga
     1381 agcaccgccg cggggacacc ggccgccagc gagagcgtcc ccggcggcgt caggcgtcgg
     1441 aacgcgggta gcagcagcgc gaggccgatc agcccgccga cgacgatcag ccgcaggtcc
     1501 tgcgccgtga gcgaggcgat gagcagtccg gcgccggccg ccaccagcag cgcgttgggc
     1561 agccgccgga tcgtcgccgc agcggcggcg tgctcctcgg gcggggcggc gtcggggatc
     1621 cggcgcaggg cgctgaccgc gaggcctccc gcgaccgcaa ggaacgggat caagccgagg
     1681 aatacgaggc gccacgtcgt gaactcgccg acgatcgccg cgattgacgg gccgaccacg
     1741 cccggcacga cccaggccgt cgacaacagc gcgaacatcc gtggctgcag ccgatcaggg
     1801 agtgtccggc cgatcgcgac gtacgccgtg gggcccaccg ccccgccgcc caggccctgg
     1861 atgaaccggg cgccgaccag gatcggcatc gacggggcca ggccgccgat cagcaggccg
     1921 atcgcgaaca gggccaggcc gacggcaaag ggcttccaga gcgccatgcg atcgagcgcg
     1981 ccgccggcaa cgacgatgcc cagcaggctg cccaggaaga acgacgagta cacccagccg
     2041 tagagctcga tctggccgag ctcctcgccg accaggggca gcaccgtccc gatcgcgagc
     2101 gactccagcg cgaccagcgt gatggtcgcg atcaggccca ccgtcaggcc ccgcagcccg
     2161 ggcgcgaaca gctcggcggt gcgcgcaggc accacgatgg cgggctgcgc ggcggcggcg
     2221 ggggcggtca cggaactgat gatgccgggt cggacgagcg cgggccgcag tcgtctgatg
     2281 cggtcgtttg cagcagatga cgtagaacgg atgacgtaca gccaatccgg cgttcagccc
     2341 gtacgctcgg gtgaatgccg gacgacatcg agtacgtcgg cctcatggcg gaggcgtggg
     2401 acgcgctccg gggggacacc tcggggtggg aggaccgtgc ctggtttcgc gaggtcatac
     2461 gccagcgggg ggaacccgtc ctcgatgtcg gcacggggac cggcagactc ctcctcgact
     2521 tcctcgccca gggcgtcgac atcgacggca tcgacaacgc gcccgacatg ctggaacgcc
     2581 tccacgcgaa ggccgcggcc gcgggcctgg acgtggatgg gcgggtgcac ctcgggcgga
     2641 tgcagtcgct gcggctgtca cgccgttacg ggaccgtgat cgtcccctcg tcgtcgttcc
     2701 agctgctgct cgagcccgac gacgccgccg aggcgatgcg ccggttcttc gcgctgctgc
     2761 tcccgggcgg ctcgctcgcg atgccgttca tcgtcatgga caaggcgtac gaggagcact
     2821 ggacgaagga ggcggcgctc gaggacgggt cgatcgtgcg gcgcacctcc accgcgacgt
     2881 tcgatcccac cgtgggcatc gaggcgaccg acgacctgta cgaggtgctc aacggcagcg
     2941 gcccggtccg catcgagcac ttcgtccggc cacgcgcgac ccgggcctac acccgcgacc
     3001 aggcccgcgc gctgtacgag acggctggat tcgtcgacct cgagtggggc gccgacttca
     3061 cgttcgagga ccgcgagcac gcggagatct tcacgatcgt cggccgccgc ccggactgac
     3121 ctgtctcgcg cggccgctcg cccagcgttg cctggcagtc atcacttctg gcctcaacgg
     3181 ccatcccggt cgtcgttcga cacggattga tgcctgccag acacaccatc atgtcgagga
     3241 gccctggacg gcgggcctca gacgccttga gaccgatttc gttgtctgcc aggcatcgac
     3301 cgggcgatcc cggctagccc tcggcggcgg ctgccaggat gcgctcgatc tgggtgaagc
     3361 cacgcattcg cgcgtgctcg agcggcgtga cgccgtcctt gtccccgatg gtgacgtcgg
     3421 ccccgtgatc gaccagcagc tggacgacct gctggtgccg ctcgtcgccg ttgctcagga
     3481 cgatcgcctc cagcagcgcg gtccagccga ggttgttgac gtggtcgatg tcaacctcgg
     3541 tgcgcgtcag cagctcctcg acgacctcca cgtggccgcg ctcggacgcg gggatcaggg
     3601 cggtcccgcc gaagcgattg gtgagtgcgg gatccgcgcc cgcgtcgatg gcgagccgca
     3661 ggatgtccag caggccctcc gcgccggcgt acaggaatgg gttgtcgagg cggtcgtcac
     3721 ggagatcgat gtccgcgccg gcctcgatga gcgcacggac ggtgtccacg tggttgccgc
     3781 gggtggccgt catcaccggc gtgcgaccgt cctcgtcgcg ctcgttgacg tcggctcctg
     3841 cctcgatcag ctccaggacg ctccgggtat cgccacgcgc ggcggccttg ccgagctggt
     3901 caaccacggt cggcaccggg gacgcgcccg cggacccaca agccgcgagg acaacgatcg
     3961 caaccaggca caacaacctc accttcgaac cggacctacg ttcccgccag ccgctccacg
     4021 acgggggcaa gggtatccag atcaccgacc atgatggagc tgatgcccag ccgctcgcgc
     4081 tgctcccgga gcttcgtcac cagtccgtcc accgacccga tgaacacgtg tggggactcg
     4141 aggaactcgt cgagcgtgat ctcgatccc
//
LOCUS       DBNHXO010000426         5895 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5088723, whole genome shotgun sequence.
ACCESSION   DBNHXO010000426 DBNHXO010000000
VERSION     DBNHXO010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5895)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5088723"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..998)
                     /locus_tag="AC162A_08610"
     CDS             complement(6..998)
                     /locus_tag="AC162A_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIR5010941.1"
                     /translation="MPNLLQRPRLRPGRAVDIAVGPPAEEVEPDETSAAGVRWLNIDR
                     PREVDREWLEREFGFHHLAIEDVASRNQRPKLDAYDDYLFIVLHFPVFDKPSGRLLTA
                     ELDLFVGPDFLITLPDQNVPPLAAMFERYREREEVREATFSKGTGYLLYRIVDTCVDA
                     SFPMLRKMGAKLDQLEDDIFEGRSDKIVQEISNAKQEIINFRRVVRPMRAVLRDLERT
                     KQRYLQEDLDIYFDDITDAAERIWDVLENYKEVAEALEDSNESVLSHRLNESLRVLTA
                     FSVALLPLTLLASIFGMNFDLPGEEGLPEFWVVILFMGLILASVLLFFRRRGYL"
     gene            1160..1714
                     /locus_tag="AC162A_08615"
     CDS             1160..1714
                     /locus_tag="AC162A_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5010942.1"
                     /translation="MQQELARRAREGDHDAFSALVRETLGRLYTTARLILRDPDRAED
                     AVQDALIEAWRDIRGLRDIDRLDAWLNRLLVRSCYRAAKRERRRTLTEIPLGPFHDGT
                     RPDSVLPLADRDEIERAFQRLTQDQRTVLTLVYFADLSLADAAVALGIPIGTTKSRLH
                     NALIALRAALAATERVPELKGGVA"
     gene            1711..2727
                     /locus_tag="AC162A_08620"
     CDS             1711..2727
                     /locus_tag="AC162A_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010943.1"
                     /translation="MSAFDTMPTDDLERRLSSWMGGQGPDTDLTSTYDTIIDATRGAR
                     QRPWFLVRRGIRRTTPMSERRFATERALTAGLAVLLVVLAIGAGLVIGGRLLTSPDES
                     TPAPSESSSAPTSVDALAIENGISLSPGQLATSRLFQPTLTFRPSPRTPGESAVDLCP
                     SVESSSRSLVLAHPKGCVEDLRFIRPWAVDCGSAGEHPDADALATAILAIPSTSGSTD
                     LGDLQSAGAVPPAMFADQYHGRVVEMLGYNPLFQADVVNRDDCRLLPEPGSGDPVIEI
                     RRDLSALFVLIDVNGELIVIRASVAGHDAASGGEAQSRGYGQGGSEQLRHLLGLVYDV
                     RFGP"
     gene            2989..4296
                     /locus_tag="AC162A_08625"
     CDS             2989..4296
                     /locus_tag="AC162A_08625"
                     /inference="COORDINATES: protein motif:HMM:NF020416.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HIR5010944.1"
                     /translation="MTKGVITVDPPEAEIGDPVSIRLSAFPPQHPVTVRATTVGLAYP
                     DLEDTGTVRESSATFQTDASGTVDLAEDAPISGSYAVANPMGLFWSMTEVQAASEPTS
                     ASPPPDSANPAVFLQYRTELTAEVDGAPVATAVLDQDLGSPDVTMTEISENGVLGQFY
                     LPPGAGPFPAVIVLSGSSGGLTVRRPKVFASQGYAVLSLPYFNYTSPVDGTALPSTAV
                     ELPLEYFGKAIDWLQAQPSVDPDRIGIYGTSLGGQVAMLVGARYPEIKSVIAIAPPTV
                     TWDDGAGKSSFSFEGKPVPFATTIGVEAAAQPFRDAVAAGQDFRATIPGIVANAKADP
                     ETAAAIIPVEKIKGSVLVVSGTNDTQLPSVVYGELSMDRLREHDFAFPYRHVIGEGAG
                     HLIDVPYVDRSTEISEGGGSPEANELAGEAMWPLVLEDLAAMK"
     gene            4381..4938
                     /locus_tag="AC162A_08630"
     CDS             4381..4938
                     /locus_tag="AC162A_08630"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5010945.1"
                     /translation="MQRDLVVRAQRGDVDAFSALTAGLTQRLYSAARLILRDDERAAD
                     AVQDALLRAWLDIRGLRDPEKFEAWLHRLFVRACYRAADRHRSREVVELRVAMTADTV
                     TSDAQGDIAARDQLDRGFRRLTPEQRAVIALHHYLGLTLAESAEVLGIPLGTMQSRLS
                     RATQALRAALEADERPTAVVAEAVR"
     gene            4935..>5895
                     /locus_tag="AC162A_08635"
     CDS             4935..>5895
                     /locus_tag="AC162A_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5010946.1"
                     /translation="MTSYDRFDRDLAVWLDAETALAAPAGLHAGAMEQARHTHQRPAW
                     LVAVRGGTMGRAAAPALDRRAVYALAVLGLLLAVVLAALVAGALRNDRHRLGANGSIT
                     YTFQGNNHGPGGTWQVGPDGTGAGQIKGSGGCPKFSRDGGVMVTATGDSSLTTLLVTD
                     TAMGRVTSSLRAENLDYYWHPHFGSYDLSPDGTRIAWLKSADETEDRPSREIWVSSIA
                     DDTARLLVPAADDPAVRYGVPLWSPNGRRVAFETGVAGAEGGYGTRVSISIVDVDTGE
                     VHQLSTRPATTWGGLSWSPDGRLVAFNGQPDGVPIPTPDPEQER"
BASE COUNT          893 a         2055 c         2056 g          891 t
ORIGIN      
        1 gtctgctaca ggtacccgcg gcggcggaag aacagcagga cgctggccaa gatcagcccc
       61 atgaagagga tcacgaccca gaactccggc aggccttcct cgcccggcag gtcgaagttc
      121 atcccgaaga tgctggcgag cagcgtgagc ggcaggagcg cgacgctgaa cgcggtgagc
      181 acgcgcagcg actcgttcag gcggtgcgag agcacggact cgttggagtc ctcgagcgcc
      241 tccgcgacct ccttgtagtt ctcgaggacg tcccagatcc gctcggcggc gtcggtgatg
      301 tcgtcgaagt agatgtcgag gtcttcctgg aggtagcgct gcttcgtgcg ctccaggtcg
      361 cgcagcacgg cgcgcatcgg ccggacgacc cggcggaagt tgatgatctc ctgcttcgcg
      421 ttcgagatct cctggacgat cttgtcggac cgcccctcga agatgtcgtc ctcgagctgg
      481 tcgagcttgg cgcccatctt gcgcagcatc gggaacgagg cgtcgacgca ggtgtcgacg
      541 atccggtaca gcaggtagcc cgtgcccttg gagaaggtcg cctcacgcac ctcctcgcgc
      601 tcccggtagc gctcgaacat cgcggccagc ggcggcacgt tctggtcggg cagcgtgatc
      661 aggaagtcgg ggccgacgaa caggtcgagc tcagccgtca gcaggcggcc gctcggcttg
      721 tcgaacacgg ggaagtggag aacgatgaac aggtagtcgt cgtaggcgtc gagcttgggg
      781 cgctggttgc gcgacgcgac gtcctcgatc gcgaggtggt ggaagccgaa ctcccgctcc
      841 agccactcgc ggtcgacctc gcgagggcgg tcaatgttca gccagcggac gccggcggcg
      901 ctggtctcgt cgggctcgac ctcttcagcc ggcgggccga cggcgatgtc gacggcgcgg
      961 cccggccgca ggcgcggccg ctgcagcagg ttcggcactt ggagggaccc cgttcggcat
     1021 ggggcgatcc taacccagcc cgctcgtgcg tggcctacga tccggccagc accgccagcc
     1081 agaatgaacc tcgaggcacc gtcgacgccc cggtagaggg tgaggcccgc agtcgtgggt
     1141 cgggacgagg agccgccgga tgcagcagga gctcgcgcga cgagccaggg agggcgatca
     1201 cgacgccttc tcggccctcg tgcgcgagac gctgggccgg ctgtacacga cggcccgcct
     1261 catcctccgt gatcccgacc gggccgagga cgccgtccag gacgccctga tcgaagcctg
     1321 gcgcgacatc cgcggcctgc gcgacatcga ccggctcgac gcgtggctca accggctgct
     1381 ggtccggtcg tgctaccggg ccgcgaagcg cgaacggcgg cggaccctca ccgagatccc
     1441 cctcgggccg ttccacgacg ggacgcgacc cgacagcgtc ctgccgctgg ccgaccgcga
     1501 cgagatcgag cgggcgttcc agcggctgac acaggaccag cgaacggtcc tgaccctcgt
     1561 gtacttcgcg gatctctcgc tcgccgacgc tgcggtcgcg cttgggatcc cgatcggaac
     1621 gaccaagtcg cgcctccaca acgcactgat cgccctgcgg gcggccctcg ctgccacgga
     1681 gcgcgtcccc gagctgaagg ggggcgtcgc atgagcgcct tcgacaccat gccgaccgac
     1741 gatctcgagc ggcgcctcag ctcgtggatg ggcggccaag gcccggacac ggacctgacc
     1801 tcgacctacg acacgatcat cgatgcgacg cgcggcgcgc ggcagcgccc gtggttcctc
     1861 gtccggcgcg ggattcggcg aacgacgccg atgagcgagc ggcgcttcgc aaccgagcgc
     1921 gcgctgacgg ccgggctggc ggtgctgctc gtcgtactgg cgatcggcgc cggcctcgtc
     1981 atcgggggtc gcctgctgac cagcccggac gagtcgaccc cagctccatc ggaatccagt
     2041 tctgcgccga cctcggtgga cgcgcttgcg atcgagaacg ggatatcgct ctcgccaggt
     2101 cagctcgcga cgagccgcct gttccaaccg accctgacct tccgccccag tccgcgcacg
     2161 cccggggagt ccgctgtcga cctctgcccg tccgtcgagt cgagctcgcg ctccctcgtg
     2221 ctggcgcatc cgaagggctg cgtcgaagac ctgcgattca tccgcccctg ggccgtggac
     2281 tgtgggtcgg ccggcgagca cccggacgcc gacgcgctcg cgaccgccat cttggcgatc
     2341 ccctcgacca gtggctccac ggacctcggc gacctccagt cggccggcgc ggtcccgccc
     2401 gcgatgttcg ccgaccagta ccacgggcgc gtcgtggaga tgctcggcta caacccactg
     2461 ttccaggccg acgtcgtgaa ccgggacgac tgcaggctgc tcccggagcc cggcagcggc
     2521 gatcccgtga tcgagatccg tcgtgacctc agcgcgctgt tcgtgctaat cgacgtcaac
     2581 ggcgagctca tcgtcatcag ggccagcgtc gcgggccacg acgcggcgag tggtggcgag
     2641 gcgcagtcac gcggctacgg ccaaggaggg agcgagcagc tgcggcatct cctcggcctt
     2701 gtctacgacg tccggttcgg gccctagtcg tgacgggtca cccggggaga tagcgatgtc
     2761 tcgtcgaatc ccgtgtagta gcgtggacgg ccccatccag cgagccgttg gtgccggagc
     2821 tcagcgatga aggcgatgcg acgctcggcg ttggtcctga ccatggttct ggcgggctgc
     2881 ggttcgtcga tgccgccgga tcctggcaca tcggcctccg cgccatcatc ggcctcattc
     2941 gtgccgtcgg cccccctcgc gacagcatcg ccgggcgcga gctccgccgt gaccaagggc
     3001 gtcatcaccg tcgacccccc tgaggcggag atcggcgatc cggtttcgat ccggctgtcc
     3061 gcgtttccgc cacaacatcc ggtcaccgtc cgggcgacga ccgtcggcct cgcgtatccg
     3121 gatcttgagg acaccggaac ggttcgcgaa tcctccgcga cgtttcagac tgacgcgagc
     3181 gggacggttg acctggcgga ggacgcgccc atcagcggtt cctacgctgt cgccaatccg
     3241 atgggtctgt tctggtcgat gacggaggtc caggccgcga gcgagccgac gtcggcgtcg
     3301 ccgccaccgg attcggccaa tcccgcggtc ttcctccagt atcgaaccga gctcacggcg
     3361 gaggtcgacg gagccccggt agccacggcg gtccttgacc aggatcttgg ttcgccggac
     3421 gtgacgatga cggagatctc tgagaacggc gtgctgggtc agttctacct gcctccgggc
     3481 gcggggccct ttccggcggt gatcgtcctg tccggcagca gcggtggcct gaccgtcaga
     3541 cggccgaagg tgttcgcgag tcagggctac gccgttctgt cgctgccgta cttcaactac
     3601 acctcgccag tcgatgggac ggcgttgccg tcgaccgcgg tcgagctccc gctggagtac
     3661 ttcggcaagg cgatcgactg gctgcaggcc cagccgagcg tcgacccaga tcggatcggg
     3721 atctacggca cgtcgctggg cggtcaggtg gcgatgctcg tcggcgcgag gtacccggaa
     3781 atcaagagcg tgatcgcgat cgcgccgccg acggtcacct gggacgacgg ggcgggcaag
     3841 tcgagcttca gcttcgaggg caagcccgtc ccgttcgcca cgacgattgg tgtcgaggcc
     3901 gcggcgcagc cgttccgtga cgccgttgcg gcggggcagg acttccgggc gaccattccg
     3961 ggcatcgtcg cgaacgcgaa ggcggatcct gagaccgcgg ccgcgatcat ccccgtcgag
     4021 aagatcaagg gatccgtgct cgtcgtctcg gggaccaacg acacccagct gccgagcgtg
     4081 gtgtacggcg agctgtcgat ggaccggctc agggagcacg acttcgcgtt cccgtatcgc
     4141 cacgtcatcg gcgagggtgc aggtcacctc atcgacgtcc cctacgtcga ccggtcgacc
     4201 gagatctccg aggggggcgg gagtccggag gcaaacgagc tcgccggcga ggcgatgtgg
     4261 ccgctcgtgc tcgaggacct ggcagcgatg aagtagtcag gcgcggatgg aacaatccgc
     4321 cgggtcgacg gcccagacta ggcgagcgcg gccgagtggc cgccacgacc agggcgaccg
     4381 atgcagcgag acttggtggt cagggcgcag cgaggcgacg tcgacgcctt ctccgcgctg
     4441 accgccggcc tgacgcagcg cctgtactcg gcagcccggc tgatcctgcg cgacgacgag
     4501 cgggcggcgg acgcggtcca ggatgccctg ctccgggcgt ggctcgacat ccgcgggctg
     4561 cgcgaccccg agaagttcga ggcctggctc caccggctgt tcgtccgcgc atgctatcgg
     4621 gccgccgacc gccaccggag tcgcgaggtg gtcgagctgc gcgttgccat gaccgccgac
     4681 accgtgacct ccgatgcgca gggcgacatc gccgcgcgag atcagctgga tcgcgggttc
     4741 cggcgcctca cgcccgagca gcgggcggtg atcgccctcc atcactacct gggcctgacg
     4801 ctcgccgagt cggcggaggt gctcggcatc ccgttgggca cgatgcagtc ccgcctcagt
     4861 cgcgccaccc aggcgctgcg cgccgcgctc gaagccgacg agcgaccgac ggccgtcgtc
     4921 gcggaggccg tccgatgacc agctacgacc gcttcgaccg cgacctcgcg gtctggctcg
     4981 acgccgagac cgccctggcc gctcccgccg gccttcacgc gggggctatg gagcaggcgc
     5041 gtcacaccca ccagcgaccg gcctggctgg tggcggttcg cggcgggacg atggggcggg
     5101 cggccgcccc ggccctcgac cggcgcgcgg tctacgcgct ggccgtgctc ggactgctgc
     5161 tcgccgtggt gctggcagcc ctcgtcgccg gcgccctgcg caacgatcga catcgactcg
     5221 gtgccaacgg ctcgatcacc tacaccttcc agggcaacaa ccacggcccg ggcgggacgt
     5281 ggcaggtcgg tcccgacggg accggcgccg gccagatcaa gggcagcggc ggctgcccga
     5341 agttctctcg cgacggtggc gtgatggtca cggcgaccgg agacagctcc ctcacgaccc
     5401 tgttggtgac cgacactgcg atgggccgcg tgacatcctc gctccgcgcc gagaacctcg
     5461 actactactg gcatccgcac ttcggctcgt atgacctctc gcccgacggc acccggattg
     5521 cctggctgaa gtccgccgac gaaaccgagg accggccgag ccgcgagatc tgggtctcat
     5581 cgatcgccga cgacaccgcg aggctattgg tgccggcggc cgacgacccg gcggtacgtt
     5641 acggcgtgcc cttgtggtcg ccgaacggtc gcagggtcgc cttcgagact ggggtcgcgg
     5701 gagccgaggg agggtacggc acccgagtga gcatctcgat cgtcgatgta gacacgggcg
     5761 aggtccacca gctctcgacc cggccggcga ccacctgggg cggcctctcg tggtcgccgg
     5821 atggtcgcct cgtcgccttc aacgggcaac ccgatggggt gccgatcccg acgcccgacc
     5881 ccgagcagga gcgcg
//
LOCUS       DBNHXO010000427         2035 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1318904, whole genome shotgun sequence.
ACCESSION   DBNHXO010000427 DBNHXO010000000
VERSION     DBNHXO010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1318904"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="AC162A_08640"
     CDS             <1..977
                     /locus_tag="AC162A_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010947.1"
                     /translation="VQTQFRYGTTAETIAAALTPLLVARPVPTDPEPSPVATPASTHP
                     AAMVPMGDLRATVVSTSVWAGGGSPVILSLADAMGMPLDGSTPVTARVVGADGAVVGP
                     DAPAVPIRPAGENQVSYVANVDIPSPGSWRLDLVAADGTSGSVAVTALDQGASARLGG
                     PAPDVDTPTLADVGGNLLEISTQPQSDARLYQSSTADARAAGRPYVIVIDSARFKVSP
                     ACGRALSMIRYLIDRWPDVTFIHLEPFPYQIVTGEPVLDGDIASPPVNALTRAFGLGD
                     ATWPSTAMPWIFVVDGNGVVRAKATGVIGSADVDLALSQVTGEGVIGN"
     gene            complement(974..1306)
                     /locus_tag="AC162A_08645"
     CDS             complement(974..1306)
                     /locus_tag="AC162A_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010948.1"
                     /translation="MATNPHLVLPAVLAPRVHAGPLEAPVNAEQRSYRAPAVSPGPSR
                     GDNQAYAAAELVRLGFATRVTLVNAPVDDALPDTWEIRGTPVGLVRLPASRSRLTAGP
                     VTASRTRG"
     gene            complement(1376..>2035)
                     /locus_tag="AC162A_08650"
     CDS             complement(1376..>2035)
                     /locus_tag="AC162A_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5010949.1"
                     /translation="LREHGFRVTTAETAAAGLAAWAADRADVILLDLGLPDRDGDVVI
                     RQVRKESTVPILVISARSQESDKVQALEIGADDYVTKPFGLAELRARVAALLRRAGGA
                     DADQAGLVRAGPLVLDPGRRSITCDGAPLDLTPREYELLKTMIAHRGRLLTKGRLLRA
                     VWGTAYGDEGHYLHVYVSRLRRKLDAADPTGRASGLIVAEPGVGYRIGSGDDEATEAK
                     A"
BASE COUNT          267 a          738 c          757 g          273 t
ORIGIN      
        1 atgtgcagac gcagttccgg tacggcacca cggccgagac gatcgcggct gcgctgacgc
       61 cgctgctggt cgcccggcct gtcccgacgg acccggagcc cagcccggtc gcgacacccg
      121 cctcgacgca tccggctgcc atggtcccga tgggcgacct gcgcgcgacc gtggtctcga
      181 ccagcgtctg ggccggcggc gggagcccgg tgatcctgtc gctcgccgac gccatgggca
      241 tgcccctcga cggctcgacg ccggtgacgg cgcgggtcgt gggcgcggat ggagcggtgg
      301 tcgggccgga cgcccctgcc gtcccgatcc ggccggccgg cgagaatcag gtctcgtacg
      361 tcgcgaacgt cgacatcccg tcacccggca gctggcgcct cgacctcgtc gccgccgacg
      421 gcacgagcgg gtcggtcgcg gtcaccgcgc tggaccaggg cgcctcggcg cggttgggcg
      481 gaccggcgcc ggacgtcgac accccgacgc tcgccgatgt gggcggcaac ctgctcgaga
      541 tctcgaccca gccgcagtcg gacgcgcgcc tctaccagtc gtcgacggcc gacgcccgcg
      601 cggcgggccg gccgtacgtg atcgtcatcg actcggcgcg gttcaaggtc tcgccggcct
      661 gcggcagggc gctgtcgatg atccggtacc tgatcgatcg ctggccggac gtgaccttca
      721 tccacctcga accgttcccg taccagatcg tgacgggcga gccggtcctc gacggggaca
      781 tcgcgagccc gccggtcaat gcgctcaccc gcgccttcgg gctgggcgat gcgacgtggc
      841 cgtcgacggc gatgccctgg atcttcgtgg tcgacggcaa cggggtcgtg cgagccaagg
      901 cgacgggggt gatcgggagc gccgatgtcg acctcgcgtt gagccaggtc accggcgagg
      961 gcgtgatcgg gaactagccg cgggtccggg acgctgtgac aggccccgcc gtgagccgcg
     1021 agcggctggc cgggagccgg acaagcccca ccggcgtgcc gcggatctcc caggtgtcgg
     1081 gcagcgcatc gtcgacgggc gcgttgacga gcgtcacgcg ggtcgcgaag cccagccgga
     1141 cgagctcggc ggccgcgtac gcctggttgt cgccgcgcga cgggccggga gatacggcgg
     1201 gtgctcgata cgagcgctgc tcagcgttga ccggcgcctc aagcggtcct gcatgcaccc
     1261 ttggtgcgag gacggcgggc aggacgaggt gcgggttggt agccacgctt ccaacctagg
     1321 cgccgtgccc aagaggaaac cgcctccccg agccccgcga gcgctcaaca agcgctcaag
     1381 ccttcgcctc ggtagcctcg tcgtccccgc tgccgatccg gtagccgacg cccggctcgg
     1441 cgacgatcag gccgctcgcc cggccggtcg ggtccgccgc atcgagcttg cgccggaggc
     1501 ggctgacgta gacgtggagg tagtggccct cgtcgccgta ggccgtgccc cacaccgcgc
     1561 gcagcagccg gcccttggtg agcagccggc cgcggtgggc gatcatcgtc ttgagcagct
     1621 cgtactcgcg cggggtgagg tcgaggggcg cgccgtcgca ggtgattgac cgccggcccg
     1681 ggtcgagcac gagcggcccg gcgcgcacga ggccggcctg gtccgcgtcg gcgccgccgg
     1741 cccgccgcag cagggcggcg acacgggccc ggagctccgc cagcccgaac ggcttggtga
     1801 cgtagtcgtc ggcgccgatc tccagggcct gcaccttgtc cgactcctgg ctgcgcgccg
     1861 aaatgacgag gatcgggaca gtcgactcct tgcggacctg gcggatcacg acgtcgccgt
     1921 cacggtcggg caggccgagg tcgagcagga tcacgtccgc gcgatcggcc gcccacgccg
     1981 cgagaccggc cgctgccgtc tcggcggtcg tcacccggaa cccgtgctcg cggag
//
LOCUS       DBNHXO010000428         1879 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5486415, whole genome shotgun sequence.
ACCESSION   DBNHXO010000428 DBNHXO010000000
VERSION     DBNHXO010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5486415"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1414)
                     /gene="mltG"
                     /locus_tag="AC162A_08655"
     CDS             complement(29..1414)
                     /gene="mltG"
                     /locus_tag="AC162A_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR5010950.1"
                     /translation="MSVRGGQGPRDPIASPDPTSSKRVNPYQRTGRYGRGPGDQRRYE
                     RYGDQRSGVGAVLRFLLFLFILAAVVLVVMATVARPLVRAVLVPWAWSNPSALELAFV
                     SDLVREDLGTALSDPASADATEVEFVVEPGDTPATLAPKLEEAGIIKNQRAFLYEARQ
                     DELLPKLNAGRFSLALNLTPAGVVDGLVNNRIENHTVVVTFREGLRAEQMAAKLETVT
                     GTNVDAKAFYNLVMKPTDALLGDYPWLLDENIRPKGASLEGFLYPATYTLRVGDEDPT
                     DAEDLVRMMLDAFHDRVGAERLEVPDKRGLTFYQVLTLASIVEREAVLDDERAKIAGV
                     YQNRIDRVPSVAHGLLQADPTVIYAVDTVNLGKFSPDWAKYVFWTVPKDGIKGQVLPE
                     SLAGFDTYQVRGLPPAPIATPTIASIDAALRPDTKSKYTYFVAIPDGNGAHDFSKSLA
                     EHEQKLREYGY"
     gene            complement(1411..1869)
                     /gene="ruvX"
                     /locus_tag="AC162A_08660"
     CDS             complement(1411..1869)
                     /gene="ruvX"
                     /locus_tag="AC162A_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR5010951.1"
                     /translation="MSRILGVDLGERRIGLAVAESAIGGARPLATIARAKTIESDADA
                     LARVCREQDVIELVIGLPVEASGVEGPMAQGARAWAAEIGTRLALPVALRDERLSSFE
                     AERRLGRMPRGRSGGAPSRAQRNAFRARIDREAAAVILQDELDARRAEAR"
BASE COUNT          262 a          648 c          683 g          286 t
ORIGIN      
        1 cagcgacgcc gctcgcgacg cggtcaccct agtagccgta ttcgcggagc ttctgctcgt
       61 gctccgcgag cgacttgctg aagtcgtgcg cgccattccc gtccgggatc gcgacgaagt
      121 acgtgtactt gctcttggta tcggggcgga gcgcggcatc gatcgaggcg atcgtcggcg
      181 tggcgatcgg ggcgggcggc aggccgcgca cctggtaggt gtcaaagccg gccagggact
      241 cgggcaggac ctggcccttg atgccgtcct tgggcaccgt ccagaacacg tacttggccc
      301 agtccgggct gaacttgccc aggttgacgg tgtcgacggc gtagatgacc gtcgggtcgg
      361 cctggagcag gccgtgggcc acgctcggca cccggtcgat gcggttctgg tagacgccgg
      421 cgatctttgc ccgctcgtcg tcgagcaccg cctcgcgctc gacgatcgag gccagcgtca
      481 gcacctggta gaaggtgagc ccgcgcttgt cgggcacctc gaggcgctcg gccccgacgc
      541 gatcgtggaa tgcgtccagc atcatccgca cgaggtcctc ggcgtccgtc ggatcctcgt
      601 cgccgacccg gagcgtgtag gtcgccgggt agaggaagcc ctccagcgaa gcgcccttgg
      661 gcctgatgtt ctcgtccagc agccaggggt agtcgccgag cagcgcgtcg gtgggcttca
      721 tcacgaggtt gtagaacgcc ttcgcgtcga cattggtgcc cgtcaccgtc tcgagcttgg
      781 ccgccatctg ctcggcgcgc agcccctcgc ggaacgtcac gacgaccgtg tggttctcga
      841 tgcggttgtt gaccaacccg tcgaccaccc ccgctggggt caggttgagc gcgagggaga
      901 atcggccggc gttgagcttc ggcagcagct cgtcctggcg cgcctcgtac aggaacgcgc
      961 gctggttctt gatgatcccg gcctcctcga gcttcggcgc gagggtggcc ggagtatctc
     1021 cgggctcgac gacgaactcg acctctgtgg cgtccgccga ggcggggtcg gacagcgccg
     1081 tcccgaggtc ctcgcgaacg aggtccgata cgaaagcgag ctcgagggcg gacgggttgc
     1141 tccaggccca cgggaccagg accgcgcgca cgagcggccg ggcgaccgtc gccatgacga
     1201 ccagcacgac ggcggcgagg atgaacagga acagcaggaa ccgcaggacc gcgccgacgc
     1261 cactgcgctg gtcgccgtag cgctcgtacc ggcgctggtc gccgggtccg cggccgtaac
     1321 ggccggtgcg ctggtacggg ttgacgcgct tgctcgaggt cgggtccggg gacgctatcg
     1381 gatctcgcgg gccctggccg ccacggacgc tcaccgcgcc tccgctcgcc gggcgtccag
     1441 ctcgtcctgc aggatcacgg ccgcggcctc gcggtcgatg cgcgcccgga aggcgttgcg
     1501 ctgcgcccgt gacggggcgc cgccggaccg gccgcggggc atacgcccga ggcgacgctc
     1561 ggcctcgaac gaggacaggc gctcgtcgcg gagcgcaacg ggcagggcaa gacgcgtgcc
     1621 gatctcggcc gcccaggcgc gggcgccttg ggccatcggc ccttcgacac cgctcgcctc
     1681 gaccggcagg ccgatgacga gctcgatgac gtcctgctcg cggcacacgc gggccagggc
     1741 gtcggcgtcc gactcgatgg tcttggcgcg ggcgatcgtc gcgagcggcc gggcaccgcc
     1801 gatcgccgat tcggccaccg cgagcccgat ccgccgttcg ccgagatcga cgcccaggat
     1861 gcggctcatc cggcggatg
//
LOCUS       DBNHXO010000429         3471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2706590, whole genome shotgun sequence.
ACCESSION   DBNHXO010000429 DBNHXO010000000
VERSION     DBNHXO010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2706590"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="AC162A_08665"
     CDS             <1..804
                     /locus_tag="AC162A_08665"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR5010952.1"
                     /translation="ASVDDVSAFFRTWYAPNNAVLSVVGDCDRAQVRAWADKYFGPIP
                     ANDALPPPPDLSLPALIGEERRETVEDRVPLPRVYVAFRAPVFGDRRLDALDLASQIL
                     AGGKGSRMHRRLVREERLAQDVAMFTLPFIGGASINVGWVTVRPGVDIATAEAAYLAE
                     LERLAAERPTADELERAKALTEADELGALARVEERADRLSMYATLFDDPAMVNTLLPR
                     YLSVTADQIRDAAREVFRADNRVVLTYLPAAADAAPDDAPRDASDEEAA"
     gene            801..2177
                     /locus_tag="AC162A_08670"
     CDS             801..2177
                     /locus_tag="AC162A_08670"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR5010953.1"
                     /translation="MSGLELVAERPVPGEPRAYEFPATVRTQLANGLRVIVTPMPGRA
                     LVAASLVIRAGAGDEPAQLGGATVLAARALTEGTEVRDAVALTEAAERLGASLHAEAG
                     WDSTFAGLDVPGSRLVPALALLAEVLQRPAFPEREVDRLRDERLTDLLQAKADPRRRA
                     EEAFVAAIYAPSSPYHRPSGGAAETVETLTAAELRTIHDRDMDPARAAIVIAGEVEPD
                     AVIRDVEALFGGWAAASAAATPGAIDDTSAVTGRFVNVVHRPGSVQTEIRVGHPALRR
                     RQPDFHAVSVMGAILGGLFNSRLNMNLREDKGYTYGASAGFDMRRARGPFAARAAVNS
                     EVTIPALRELLAELERIREEPVSGAELRAARDFLIGVFPLRFETPGPVAGSLAGLFVH
                     ELPDDELVRYRAAIEAVTAADVQRVAREHIRPDASAIVLVGDNAQFGEALAGEDLGPV
                     TVIEDTEG"
     gene            2300..2812
                     /locus_tag="AC162A_08675"
     CDS             2300..2812
                     /locus_tag="AC162A_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010954.1"
                     /translation="MIRSIGTSVVALFLIAGASFATTAFVGGARQVDTAPAAQNEEDA
                     SDALETEEPTETADDEAAEPAETDEVETEEPSETAEADETEEPSADDSGGHHRRGHAS
                     NDVDDNSGPGKGNEEGDDDDQGENEDASDDHDDDDGDDDDDDDHGGDSGHDGSGDDDG
                     GSGHDGPGDD"
     gene            2885..>3471
                     /locus_tag="AC162A_08680"
     CDS             2885..>3471
                     /locus_tag="AC162A_08680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5010955.1"
                     /translation="MSTGAGAASSSASDLSRATPDAEDEATERARTTIVDSGATSDER
                     QVVDAILAGDAGAFRQLVERESRSVIATCTRILGDRAEAEDVAQEAFVIAYRSLASWR
                     ADGPFGAWLSRIAVRLAVRRAAQRKQVAWLDPIDASGSGDDRVRAGGVDGADPASSLL
                     RSERDAELRAAVASLDEPYREVVAMRFFAERSLLE"
BASE COUNT          491 a         1281 c         1257 g          442 t
ORIGIN      
        1 gcctcggtcg acgacgtcag cgcgttcttc cggacctggt acgcgccgaa caacgcggtg
       61 ctgtcggtgg tcggggactg cgatcgcgcc caggtgcgcg cgtgggccga caagtacttc
      121 ggcccgatcc cggccaacga tgcgctgccg cccccgccgg acctgtcgct ccccgcgctg
      181 atcggcgagg agcgccgcga gaccgtcgag gatcgcgtgc cgctgcctcg cgtctacgtc
      241 gccttccggg ccccggtctt cggcgatcgc cggctcgacg cgctcgacct cgcctcgcag
      301 atcctcgccg gcggcaaggg gagtcggatg catcgccgcc tcgtccgcga ggagcgcctc
      361 gctcaggacg tcgcgatgtt cacgctgccg ttcatcggcg gggcgtcgat caacgtgggc
      421 tgggtgaccg tccggccggg cgtcgacatc gccacggccg aggccgccta tctcgcggag
      481 ctcgaacgcc tcgcggccga gcggcccacc gccgacgagc tcgagcgcgc caaggcgctc
      541 accgaagccg acgagctggg tgctctggca cgcgtcgagg agcgcgccga ccgcctgtcg
      601 atgtacgcga ccctgttcga tgacccggcc atggtcaaca ccctgctgcc gcgctacctc
      661 tccgtgaccg ccgaccagat ccgcgacgcg gcccgcgagg tgttccgagc ggacaaccgc
      721 gtggtcctga cctacctgcc cgccgccgcg gacgccgccc ccgacgacgc cccgcgcgac
      781 gcgtccgacg aggaggcggc atgagcggcc tcgagctcgt cgcggagcga ccggtcccgg
      841 gcgagccgcg ggcctacgag ttcccagcca ccgtccggac gcagctcgcc aacggcctgc
      901 gtgtgatcgt cacgccgatg cccggtcgcg cgctcgtggc ggcgtccctg gtcatccgcg
      961 cgggtgccgg cgacgagccc gcccagctgg gtggcgccac ggtcctcgcg gcccgcgcgc
     1021 tcaccgaggg caccgaggtc cgcgacgccg tcgcgctgac cgaggccgcc gagcgcctcg
     1081 gcgcgtcgct ccacgccgag gccggctggg actccacgtt cgccgggctg gacgtgccgg
     1141 ggtcgcggct cgtgcccgcg ctggcgctgc tggccgaggt cctccagcgt ccggcgttcc
     1201 cggagcgcga ggtcgaccgc ctcagggacg agcggctgac ggacctgctc caggccaagg
     1261 cagatccgcg gcgccgcgcc gaggaggcat tcgtggcggc gatctacgcg ccgtcgagcc
     1321 cctaccaccg gccctcgggt ggagcggcag agacggtcga gacgctgacg gccgccgagc
     1381 tgcggacgat ccacgaccgc gacatggacc ctgcccgcgc cgcgatcgtg atcgccggcg
     1441 aggtcgagcc ggatgccgtc atccgcgacg tcgaggcgct gttcggaggc tgggcggcgg
     1501 cgtcggccgc ggcgaccccc ggcgccatcg acgacacctc cgccgtcacc ggccgcttcg
     1561 tcaacgtcgt ccatcgcccg gggtctgtcc agaccgagat ccgggtcgga catccggcgc
     1621 tgcgccggcg gcagccggac tttcacgccg tctcggtgat gggcgcgatc ctcgggggcc
     1681 tgttcaactc gcggctcaac atgaacctgc gcgaggacaa gggctacacg tacggcgcga
     1741 gcgccggctt cgacatgcgc cgcgcccgcg gccccttcgc cgcgcgtgca gccgtgaact
     1801 ccgaggtcac gatccccgcg ctccgcgagc tgctcgccga gctcgagcgg attcgcgagg
     1861 agcctgtctc gggcgcggag ctgcgcgcgg cccgcgactt tctcatcggc gtgttcccgc
     1921 tccgcttcga gacgccgggt ccagtggccg gatcgctggc cggtctgttc gtgcacgagt
     1981 tgcccgacga cgagctggtc cgctatcggg cggcgatcga ggcggtcacg gcggccgacg
     2041 tccagcgggt cgcgcgcgag cacatccgcc ccgacgcgtc ggccatcgtg ctcgtcggcg
     2101 acaacgccca gttcggcgag gcgcttgcgg gcgaggatct cggcccggtc acggtgatcg
     2161 aggacaccga gggctgacgt tcgccccggt cgcctgaaac cttttcgcgg gacggcgcct
     2221 caggtggggt agtgcggcca acaggcaggc acgcacagcc atcatcggag ttgcccaagc
     2281 gatgaagacc acgcaccgca tgatccgttc gatcgggacc tcggtcgtcg cgctgttcct
     2341 gatcgccgga gcgtcgttcg cgacgacggc cttcgtcggt ggagcgcgtc aggtggacac
     2401 cgcgccggcg gcgcagaacg aagaggacgc gagcgacgcg ctcgagaccg aggagccgac
     2461 cgagaccgcg gacgacgagg ccgccgaacc ggccgagacc gacgaggtcg agaccgaaga
     2521 gccgtcggag accgccgaag cggacgagac cgaggagccg tccgccgacg acagcggcgg
     2581 ccaccatcgg cggggccacg cctcgaacga cgtcgacgac aacagcgggc ccggcaaggg
     2641 caacgaggaa ggcgacgacg acgaccaggg cgagaacgag gacgccagcg acgaccacga
     2701 cgacgacgat ggcgatgacg acgacgatga cgaccacggt ggcgactccg gccacgatgg
     2761 gtcgggcgac gacgacggcg gatcgggcca cgacgggccc ggcgacgact gagtcgattg
     2821 gcccatccgg ccctcttggt ccatgacctc tgtcccgcga tgcgaaccgc gccgacgccc
     2881 gatcatgtcg acgggagccg gcgcggcttc atcgagcgcg agcgacctgt cgcgcgcgac
     2941 cccggacgcg gaggacgagg cgacggaacg ggcgcggacg accatcgtcg actctggagc
     3001 cacgagcgat gagcgccagg tcgtcgacgc tatcctcgcc ggggacgcgg gcgccttccg
     3061 gcagctcgtc gagcgcgaga gccgctcggt catcgcgacc tgcacccgga tcctcggcga
     3121 ccgcgccgag gccgaggacg tcgcccagga ggcattcgtg atcgcgtacc ggtcactcgc
     3181 ctcgtggcgc gccgacgggc cgttcggcgc atggttgtcg cggatcgccg tccggctggc
     3241 cgtccggcgg gccgcacagc gcaagcaggt cgcatggctc gacccgatcg acgcgtccgg
     3301 ctcgggcgac gaccgggtgc gcgccggagg cgtcgacggt gccgatcccg cgtccagcct
     3361 cctgcgatcc gagcgcgacg ccgagctgcg ggccgccgtg gcatcgctcg acgagcccta
     3421 ccgcgaggtc gtcgcgatgc gcttcttcgc ggagcgctcg ctgctcgaga t
//
LOCUS       DBNHXO010000430         1766 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4294984, whole genome shotgun sequence.
ACCESSION   DBNHXO010000430 DBNHXO010000000
VERSION     DBNHXO010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4294984"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="AC162A_08685"
     CDS             <1..624
                     /locus_tag="AC162A_08685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR5010956.1"
                     /translation="YRGSTGYGRPYRDALQGQWGIVDVDDCVAAAQFLAVRGSVDRRR
                     IVIKGGSAGGFTTLAALTTQPGVFAAGISHYGIADLELIHLDGHKFESRYDEGLLAPW
                     PEGRQVFRDRSPIHAFDRIEAPMLITQGLDDRVVPPSQLDAMVAALHGRGVPHATIRL
                     AGEGHGYRKAESRREVYAAELSFLGQVFGFTPADDIDPIHVEHLRAR"
     gene            complement(893..>1766)
                     /locus_tag="AC162A_08690"
     CDS             complement(893..>1766)
                     /locus_tag="AC162A_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR5010957.1"
                     /translation="GRRVIVADRRDFPTDRYVLQGLAAGRGLEIRWLDGDPAEGLATA
                     DVEAALGEDVALVVLSLVNYRSSAIVDLDAVEAAARSAGAHVLWDLSHAAGAIPVDLA
                     ARDVGLAVGCTYKFLHAGPGAPAWLHVAPRLQGRLRPPIQGWFAQADQFAMGPSFDPR
                     SGIAGWLTGTPPILSLVAVEEGVRVVAEAGIGAIRAKSAALGEYAVERLDEDLAPLGC
                     RLGSPRDPARRGSHVSIRHPDARALVDGLIAGGVIVDFREPDVIRFGLSPLTTSFADV
                     DRGIVALRDLLAQR"
BASE COUNT          261 a          652 c          646 g          207 t
ORIGIN      
        1 taccgcggct cgaccggcta cgggcgtccc tatcgcgacg cgctccaggg ccagtggggc
       61 atcgtggacg tggacgactg cgtcgcggcg gcgcaattcc tcgccgttcg aggatccgtc
      121 gaccggcgac ggatcgtgat caagggcggc agcgccggcg gcttcacgac gctcgccgcg
      181 ctcacgacgc agcctggcgt gttcgcggcc gggatcagcc actacggcat cgcggacctc
      241 gagctgatcc acctcgacgg ccacaagttc gagtcacgct acgacgaggg gctgctcgcg
      301 ccgtggcccg aaggccgcca ggtgttccgc gaccgctcgc cgatccacgc cttcgatcgg
      361 atcgaggcgc cgatgctgat cacgcagggc ctcgacgacc gggtcgtgcc gccatcgcag
      421 ctcgacgcga tggtggcggc cctccacggg cgcggggtcc cgcacgcgac gatccggctc
      481 gccggcgagg gccacggcta ccgcaaggcg gagtccaggc gcgaggtgta cgccgccgag
      541 ctgtcgttcc tgggccaggt cttcggcttc acacctgccg acgacatcga cccgatccac
      601 gtagagcacc tgcgcgcgcg ctgacccgcg acccgcccgt ctaccgcccg aattgtgcgg
      661 tcgacgcaca gttccggacc gagcgtgcgc cccgtgcaca ttcccgacgg cggggtcccc
      721 gcgacggtcg acaccgtgcc gcagacgcac agtcctgacg cggacgggcc gccgccggcc
      781 ggacggtgtc cgatctgtgc gtgcggcgca cgaaccggac gagactcgtg gctgggagcg
      841 ggagcgaccg gctggaatgt gcaccaggcg cacaggcgca tcggcgccgc gactagcgct
      901 gagccagcag gtcccgcagc gccacgatcc cgcggtccac gtcggcgaag gacgtcgtga
      961 gcggggacag tccgaagcgg atgacgtcgg gctcacggaa gtcgacgatc acgccgcccg
     1021 cgatgaggcc gtcaacgagc gcgcgggcgt ccgggtggcg gatggagacg tggctgccgc
     1081 gccgtgcggg atcccgcggg ctgccgagcc gacaaccgag cggcgcgagg tcctcgtcca
     1141 gccgctcgac ggcgtactcg ccaagggcgg ccgacttggc ccggatggcg ccgatgccgg
     1201 cctcggcgac cacccgcacg ccctcctcca cggcgaccag cgagaggatc ggcggggtgc
     1261 cggtgagcca gccggcgatc cccgatcggg gatcgaacga cggccccatc gcgaactggt
     1321 cggcctgcgc gaaccagccc tggatcggcg ggcgaagacg gccctggagc cgcggcgcga
     1381 cgtgcagcca cgccggcgcg ccgggtccgg cgtggaggaa cttgtacgtg cagccgaccg
     1441 cgaggccgac gtcgcgagcg gcgaggtcga cggggatcgc ccccgcggcg tgggagaggt
     1501 cccacaggac gtgggcgccc gcggaccgcg ccgccgcctc gaccgcgtcg aggtccacga
     1561 tcgccgacga gcgatagttg accagcgaca gcacgaccag cgcgacgtcc tcgccgagcg
     1621 ccgcctccac gtccgcggtc gcgaggcctt cggccgggtc gccatcgagc cagcggatct
     1681 cgagcccccg gcccgccgcc aacccctgga gcacgtaccg gtccgtgggg aagtcgcgcc
     1741 ggtcggcgac gatgacgcgt cgcccg
//
LOCUS       DBNHXO010000431         5913 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_328399, whole genome shotgun sequence.
ACCESSION   DBNHXO010000431 DBNHXO010000000
VERSION     DBNHXO010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_328399"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="AC162A_08695"
     CDS             complement(<1..291)
                     /locus_tag="AC162A_08695"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR5010958.1"
                     /translation="MSNTEPVSDGAPVAGWAPVIEDPILRTLLRPDAVLHRLATGATW
                     AEGPVWLPHDGSVIWSDVVGNRVLRWHPDGQVSVFLEPAEFENGHTLDHDGSI"
     gene            complement(355..1758)
                     /locus_tag="AC162A_08700"
     CDS             complement(355..1758)
                     /locus_tag="AC162A_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR5010959.1"
                     /translation="MPTATPIATPSTTPQPSPAAGPTAWPRTIRSERGLEPVFAPDGT
                     AYYLAGGLNARLVALDRAGNVKPGWPIEEPKGAQFGRPAVGPDGDVHVEECAGAEVGC
                     VVHRLDEAGRDVPGWPIDLPSDFACIVGSGCAPGDLTFGLDGDLYVSHPRKSRGLRVL
                     ALDASGRIKPGWPVEPPRDGLHWGDLQIGGDGTLYLLGVPDGGGTRSRIAAFGRDGSR
                     RPGWPVPVPGVREYLVGPEGTVVTWRFVDDVGELCMDPRRTVYTVLGAEGHRLPGWPR
                     GSEGSASRPAIGADGTLYYVSRTYRAYAHDLTGEIKAGWPVPAPGASGACGPETPSLA
                     PDGTVFIVGDAVWARSPDGGRRPGWPYRPRGSLVGPCFDSECYGGHQAPAFGADGTAY
                     LVAYRSAPGGVRAEIVALDSEGQVKPGWPYRLPFDANEVSVGPISVSPDGRVIVQGGG
                     WSPYILLALDPDGTLAD"
     gene            2052..2594
                     /locus_tag="AC162A_08705"
     CDS             2052..2594
                     /locus_tag="AC162A_08705"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5010960.1"
                     /translation="MDDEPGTTLTVDELVLRPWRPSDAPDVLAVCQDPEIARWVTIPQ
                     PFGPADADAFIETAMTMWRDGTGAPFAIIDADTGQLLGAVTRFGPDGHQATFGLWLAP
                     DARGRGVGTRSLRLAADWTFATTAAIRLDAFIMVGNEASNRMVERAGFQREGVARAWD
                     LHHDGVPVDCVVYSRIRSDP"
     gene            complement(2614..2817)
                     /locus_tag="AC162A_08710"
     CDS             complement(2614..2817)
                     /locus_tag="AC162A_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010961.1"
                     /translation="MTELSTKKRNAEPKSEFGLPEERKYPMPDKSHARNAKARAAQQR
                     NEGNLSAADKKRIDRKADRILDK"
     gene            complement(2873..3070)
                     /locus_tag="AC162A_08715"
     CDS             complement(2873..3070)
                     /locus_tag="AC162A_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010962.1"
                     /translation="MCLTCGCMDAHQQMGKANISYEDVKAAASENGRSVADTLDIMER
                     TELKDRKQHPREYGVEAEVRA"
     gene            3695..4078
                     /locus_tag="AC162A_08720"
     CDS             3695..4078
                     /locus_tag="AC162A_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010963.1"
                     /translation="MTSAAYFRAWRAAHPEYRARQNRLRNERRRRCGRGDRSAEFAVR
                     RSRAMPPMPGLHVGHTLFERARQIVGPRTTTLVSLEDPLYDDLVSEATLALIEGRDPA
                     DAIRAYGARERAFGRVTCPLYEQAA"
     gene            4112..4585
                     /locus_tag="AC162A_08725"
     CDS             4112..4585
                     /locus_tag="AC162A_08725"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106130 - purine phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR5010964.1"
                     /translation="MSAPATPDRDVVTWDDLDRLVAYLAERLAGTTFDVLLCITRGGL
                     VPAGMLAYRLRIRNILVAAVAFYDDEGQPGPHPTFLQVPADPLLRGQTVLIVDEVWES
                     GTTIHAVTERVIQAGGIPTTAVLHYKPRRSLVPGTPDLHAVETDNWVVYPFKAGN"
     gene            4677..5267
                     /locus_tag="AC162A_08730"
     CDS             4677..5267
                     /locus_tag="AC162A_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018503619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010965.1"
                     /translation="MESTEHRPSATEASAALSDAEASRAMLAQRIRTPPWFFTTIGVA
                     IAAQIALTAVAMSWSVGGSGIAALSTWTIAAFAAGVAVLLAVAGVQLAAFRQLNGVWV
                     GGLLSRVVLGTGALASGAYIVALGAAIWSAIDAQWWLVATSSVIGGAAYALAGRRWFN
                     AYRDEPARHARRESVVMLGLAVVAALVGLGLLLIYR"
     gene            5267..5563
                     /locus_tag="AC162A_08735"
     CDS             5267..5563
                     /locus_tag="AC162A_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIR5010966.1"
                     /translation="MAELDPHVIAPARLKLMVTLTAVSEVEFGTVRDALEVSDSVLSK
                     HLASLEDVDYVKRRKGVHRGRRTTWISLTPDGRRALDAHIDALHALIDGVTTPR"
BASE COUNT          825 a         2142 c         2028 g          918 t
ORIGIN      
        1 gatcgagcca tcgtggtcga gggtgtggcc gttctcgaac tcggcgggct cgaggaacac
       61 cgatacctgc ccgtcggggt gccagcgcag cacccggttg ccgacgacgt cgctccagat
      121 gacggacccg tcgtggggca gccacaccgg cccctcggcc caggtggcgc cggtcgcaag
      181 ccggtgcagg acggcatccg ggcggagcaa ggtccgcagg attgggtctt cgatgacggg
      241 cgcccacccg gcgaccgggg cgccgtccga gactggctct gtgttcgaca tctggacctc
      301 gcgctgcggt cgcccgacgt gtttagtgtc gtcgaaccgg gcgtgctggc gaggtcagtc
      361 tgcgagggtt ccgtcggggt cgagcgccag gaggatgtat ggcgaccatc caccgccctg
      421 gacgatcacg cgtccgtcgg gagagacgct gatcgggcca acgctcacct cgttcgcatc
      481 gaacggcagg cggtatggcc atcctggctt gacctggcct tccgaatcga gggcgacgat
      541 ctccgcccgc acgcctccag gcgctgaacg atatgcgacg aggtaggcgg tgccgtcggc
      601 gccaaacgcg ggcgcctgat gtccgccgta gcactccgag tcgaagcagg ggccaacgag
      661 gctgccgcgt ggacgatacg gccagccggg ccgccgcccg ccgtccgggg atcgagccca
      721 aaccgcgtcg ccgacaatga agacggtacc gtcgggggcc agggaggggg tctcggggcc
      781 gcacgcgccg ctggcgcccg gcgccggaac cggccatccg gccttgatct cgcccgtcag
      841 gtcgtgcgcg tacgccctgt acgtgcgcga gacgtagtag agcgtcccat cggcgccgat
      901 cgcggggcgc gacgccgatc cctccgaacc ccgaggccac ccgggcaacc tatgcccttc
      961 agcgccgagg accgtgtaca ccgttcgacg ggggtccatg cagagctccc ccacgtcgtc
     1021 gacgaacctc cacgtgacga cggtgccctc cgggccaacg aggtattcgc gaacgcccgg
     1081 caccgggacg ggccacccgg gtcgccggct cccgtcgcgc ccaaacgctg cgatccggga
     1141 cctcgtcccg ccgccatcgg gcacgccgag caggtatagc gtgccatccc cgccgatctg
     1201 caggtcgccc cagtggaggc catctcgcgg cggctcgacc ggccagcccg gcttgatcct
     1261 gcccgaagcg tcgagggcga ggacacgtag tccccggctc ttccgcggat gggacacgta
     1321 gaggtcgccg tcgagcccga aggtgaggtc gcccggcgca cacccgctgc cgacgatgca
     1381 cgcgaagtcc gagggcaggt cgatcggcca gcccggcacg tcccgtccgg cctcatcgag
     1441 ccggtgcacg acgcacccga cctccgcccc agcgcactcc tccacgtgga cgtccccgtc
     1501 cggccccacg gcgggccgcc cgaactgggc gcccttcggc tcctcgatgg gccagccggg
     1561 cttcacgttg ccggcccggt cgagcgcgac gagtcgtgcg ttgaggccgc cggccaggta
     1621 atacgcggtc ccgtcgggcg cgaagacagg ctcgagaccg cgctcgctcc tgatggtgcg
     1681 cggccatgcg gttggtccgg ccgccggact cggctggggg gtcgtgctcg gggtcgcgat
     1741 gggcgtcgct gtgggcattg tggtgggaac cgcggtcgcc gtggggctcg cggtcggtgt
     1801 acggctcgcg actggtgtgg cgacgggagg cggaggcgtc gacggcgtcg tgacggggga
     1861 cgggctcgga tccgccgcgc aagcagcgac gcaagccgcg atgacgatcg atgccacgac
     1921 tcttgcgcgc gatgggcgca tccgctggcc tccggacggt gtggcacgaa agacatccgg
     1981 cgggtcgatt ttggtgcgct cgcccgtcac gggagcacga tgcgaccggg ctcgactgcg
     2041 agaatggcca ggtggacgac gaacccggca cgaccctgac cgtcgacgag ctcgtcctcc
     2101 gcccgtggcg cccctcggat gcaccggacg tgctcgccgt ctgccaggac ccggagatcg
     2161 cccgttgggt cacgatcccc cagccgttcg ggcccgccga cgcggacgcc ttcatcgaga
     2221 ccgccatgac gatgtggcgc gatggcacgg gcgcgccgtt cgccatcatc gacgccgata
     2281 ccggtcagct gctcggcgcg gtgacgcgat tcgggccgga cggccaccag gcgacgttcg
     2341 gcctatggct ggcacccgac gcccgcgggc gcggtgttgg cacgcggtcg cttcgactgg
     2401 ccgcggactg gacgttcgcc acgaccgcgg ccatccgcct cgacgccttc atcatggtcg
     2461 gcaacgaggc atcgaaccgg atggtcgagc gcgccgggtt ccagcgcgag ggcgtggccc
     2521 gagcgtggga cctgcaccac gacggcgttc ccgtcgactg cgtggtctac tcgcggatcc
     2581 gctccgaccc gtagcccgga cagccggcgg gacctacttg tcgaggatcc ggtcggcctt
     2641 gcggtcgatc ctcttcttgt cggccgcgct caggttgccc tcgttccgct gctgcgccgc
     2701 ccgcgccttg gcgttgcgcg cgtgcgactt gtcgggcatc gggtacttgc gctcttcggg
     2761 cagcccaaac tcggacttcg gctccgcgtt gcgcttcttc gtactgagct cagtcatggc
     2821 gtgccttcct tcgacacgtg ctggggttcg ccggacggcc ggccgcagcc ggtcaggcgc
     2881 ggacctcggc ctccaccccg tactcccgcg gatgctgctt gcggtccttg agctcggtgc
     2941 gctccatgat gtcgagcgtg tcggcgacgg agcggccgtt ctcgctcgcc gcggccttga
     3001 cgtcctcata cgagatgttg gctttgccca tctgctggtg ggcgtccatg cagccacagg
     3061 tgagacacat gctcggtcct ccttcgcggg cccgcggctg cgccgttggc atgcctcgcg
     3121 ggcgaggagc acggtaggtg ccaggacggg caatgcgcgc ctgccgcgtc atgccgcggc
     3181 gttgcagaga agtgacaggc gcggcctgtg cctgcgagcg aaggtgaacg ggtcgacggc
     3241 cgccgattgc aagccgactg caacggaccg ttaaggctgg gatgagcggc cccgacctat
     3301 actcgggccc gtccggtcca ccctgagtgc ccccctcaag tggggcgcga cagtgaaccg
     3361 cttcgccccg gcagctgccc gccgcgcgca cgccggggcc catcgggtgc atcgtcgccc
     3421 accggccgtc tcgcgctcga tgccggcccc tgaccgccgc gccaagagtg ccagtacgga
     3481 ccttgcacgc ccgccgccct gcgggcgttg ggatcgtcgc gcgacctgtt catcgcagca
     3541 gtcggccttc ggtcccgcgc cgccttccat cacccgccgc gtttcggccg ctgccgtctc
     3601 gccacgccca aggagtcgtt gtgccctgct gcccccagag cgcccgagga gtcgtccgcc
     3661 gccttgaccg tcggcatcgc caggaggcgc ttcgatgacg agcgccgcct acttccgcgc
     3721 ctggcgcgcc gcccatcccg agtaccgggc ccgccagaac cgccttcgca acgagcgccg
     3781 tcgacgatgc ggtcgcggcg accgctccgc cgagttcgcc gtccgccgga gccgcgcgat
     3841 gccgccgatg ccgggcctgc acgtcggcca cacgctgttc gagcgggcga ggcagatcgt
     3901 cgggccgcgg acgacgaccc tcgtcagcct ggaagacccc ttgtatgacg acctcgtctc
     3961 cgaggcgacg cttgcgctca tcgagggtcg cgaccccgcg gacgcgattc gggcctacgg
     4021 ggctcgggag cgagcgttcg gtcgcgtgac ctgcccactc tacgagcagg ccgcctgaag
     4081 ctcggtgccc gatacccgta tcctgcgact catgtccgca ccagcgactc ccgaccgcga
     4141 cgtcgtcacc tgggacgacc tcgatcgcct ggtcgcatac ctcgccgaga ggctcgcggg
     4201 gaccacgttc gacgtcctgc tgtgcatcac ccgcgggggc ctcgttcccg cggggatgct
     4261 cgcctaccgg ctccggatcc ggaacatcct ggtcgccgcc gtcgcgttct acgacgacga
     4321 gggccagccc ggcccgcatc ccacgttcct gcaggtcccc gccgacccgc tgctgcgcgg
     4381 ccagacggtg ctcatcgtcg acgaggtctg ggagagcggg accaccatcc acgcggtcac
     4441 cgagcgcgtg atccaggcgg gcggcatccc gaccaccgcg gtcctccact acaagccccg
     4501 acgctcgctt gtcccgggca cgcccgacct gcacgcggtc gagaccgaca actgggtcgt
     4561 ctacccgttc aaggcgggca actgaccgcg aagcggtagc tggcggcggc atcgcacgcg
     4621 tcgatcgctt ccgtcggact tgacagattg gaaagtagcg cctgatactt tccgatatgg
     4681 aaagtaccga acatcgcccc agcgccacgg aagcgtcagc agccctcagt gacgccgagg
     4741 cgtcgcgcgc gatgctggcc cagcgcatca ggacgccacc ctggttcttc accacgatcg
     4801 gcgtcgcgat cgccgcgcag atcgcgttga ccgctgtcgc catgtcgtgg tcggtcggcg
     4861 gcagcggtat cgcagccctc tccacgtgga cgatcgcagc cttcgcggcc ggcgtcgcgg
     4921 tcctgctcgc ggtggccgga gtgcaactgg cggcgttccg gcaactcaac ggcgtctggg
     4981 tcggcggtct tctgagccgc gtcgtgctcg ggaccggggc tctcgcgtcg ggcgcgtaca
     5041 tcgttgcgct cggggcggcg atctggtccg ccatcgatgc gcagtggtgg ttggtcgcca
     5101 cgtcttcggt catcggcggc gccgcgtacg cgctcgccgg ccgacgctgg ttcaacgcct
     5161 accgggacga gccggcacgc catgcgcgca gggagtcggt ggtcatgctc gggctcgcgg
     5221 tggtcgcggc actcgtgggc ctcgggctgc tgctgatcta ccgctgatgg ccgagctcga
     5281 cccccacgtc atcgcgcctg cgcgactgaa gctgatggtc accctgacag ccgtctccga
     5341 agtcgagttt ggcaccgtcc gcgacgccct cgaggtcagc gactccgtcc tgtccaagca
     5401 cctcgcctcc ctcgaggacg tcgactacgt gaagcgccgc aagggcgtcc atcgcggccg
     5461 gcgcactacc tggatctcgc tcacaccgga cggacgcagg gcgctcgacg cgcacatcga
     5521 cgccctgcac gcattgatcg acggtgtcac cacgcctcgg tgaccggtgg ccagcgttgc
     5581 gtcgcgcggc gccgcgacga tagactcagg acctcccgag cgggagtaac tcagttggca
     5641 gagtgtcagc ttcccaagcc gaccgtctcc cccaacgccg ctgcccgaga catgagcccc
     5701 ggtccctagt gcgagttttg agagcgccag gggacactgg cccaggacgc gatagttgcg
     5761 tcggcaacta gtctggcgaa gcggccgatc ggatcccctt ccggtcttgc cggtgttcgc
     5821 acgacgggga gccacccgag accgatggcc agactcgcaa gtacgggcac gatgatgccc
     5881 agggactttc ctgcctcgct actcgaccgc gta
//
LOCUS       DBNHXO010000432         1608 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3698125, whole genome shotgun sequence.
ACCESSION   DBNHXO010000432 DBNHXO010000000
VERSION     DBNHXO010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3698125"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="AC162A_08740"
     CDS             complement(<1..1123)
                     /locus_tag="AC162A_08740"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR5010967.1"
                     /translation="MTSPSREPRPTVPPSRLTHSRRATDRLRQLAFLQEFAQLLTQAR
                     DWDELMRTLVDRTTVAMGVEVCSFYLLDRTGERLTLAATNGLDVTQVGRVSLEIGQGI
                     TGRAAAGRTPVMSADVTTDPRFSWVRGFDIEDLHAMLSVPLVWNEAVVGVLNAQTTEA
                     REFDADEVEFLVTIAALLAGIVEKGRLQAEAEEQLDRLSQLDAARAELLAVVTHELRT
                     PLAVVRAYVDLLADAAASDDAETSRDLVETWRAGATDQVTRLDRLVDSILASVRGEGL
                     TGLRRDPFDVVTAISATVGTLALLLRTRPVRWEPPHGALVARGDDARFRQVLEHLLDN
                     EAKYAPPEQAVSLGVWREDGEIQVYVTDDGPGVPIDDWEA"
     gene            complement(1385..>1608)
                     /locus_tag="AC162A_08745"
     CDS             complement(1385..>1608)
                     /locus_tag="AC162A_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006448317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010968.1"
                     /translation="SLYDKDLATYDEGDAFDHASAVGFIEIFGLPLRVEAARHGAVGK
                     HHGAAWTWTQPLLADLPMNVTEGAGAAAG"
BASE COUNT          223 a          566 c          590 g          229 t
ORIGIN      
        1 ccgcctccca gtcgtcgatc ggcacgcccg ggccgtcgtc ggtgacgtag acctggatct
       61 cgccatcctc gcgccacacg ccgagcgaga cggcctgctc gggcggcgcg tacttggcct
      121 cgttgtccag caggtgctcg aggacctggc ggaagcgcgc atcgtcgccg cgtgcgacca
      181 gggcgccgtg cggcggctcc cagcgcacgg gcctggtgcg gagcagcagc gccagcgtgc
      241 cgacggtcgc ggagatcgct gtgacgacat cgaaggggtc ccggcgcaag cccgtcaggc
      301 cctcgccgcg gaccgatgcc aggatcgagt cgacgaggcg gtccaggcgt gtcacctggt
      361 cggtcgcgcc ggcacgccag gtctcgacga ggtcacgcga cgtctcggcg tcgtcggagg
      421 ccgcggcgtc ggcgagcagg tcgacgtagg cgcgcacgac cgcgagcggc gtccgcagct
      481 cgtgggtcac gaccgcgagc agctcggccc gggccgcgtc cagctgggac aggcggtcga
      541 gctgctcctc ggcctcggct tgcaggcggc ccttctcgac gatgcccgcg agcagggcgg
      601 cgatggtcac caggaactcg acctcgtcgg cgtcgaactc gcgcgcctcg gtggtctggg
      661 cgttgagcac gcccaccacg gcctcgttcc agacgagggg gacggagagc atcgcgtgga
      721 gatcctcgat gtcgaagcca cgcacccagg agaagcgggg atcggttgtg acgtcggccg
      781 acatgaccgg ggtgcgaccc gccgccgcgc gacccgtgat gccctggccg atctcgaggc
      841 tgacgcgccc cacctgcgtt acgtcgaggc cgttggtcgc ggctagggtc agccgctccc
      901 cggtccggtc gagcaggtag aacgagcaga cctcgacgcc catcgcgacg gtcgtgcggt
      961 cgacgagcgt gcgcatcagc tcatcccagt cgcgcgcctg ggtgaggagc tgcgcgaact
     1021 cctggaggaa ggcgagctgg cgcagccggt cggtcgcccg gcggctgtgg gtcaggcggc
     1081 tgggcgggac ggtcggccgg ggttcgcggg acggcgaggt cacgccttca ccctacggca
     1141 tccggcgcgg tcctcggcgg gccgtgcgcc gggcgcacat tcctgaccga gcggcgtccg
     1201 ttcttgacgc catctcgtcg gtatcgtgtt gtggcagcac gaaacgagca ccccgtcccc
     1261 gcggcggtcc agatctgtgc ggcacatgca cacacgtgac cgtcggaagg ccgtccgcgt
     1321 cgagaatgtg cacctgcggc acgctcgagc caaatgcgtg cgcacggcgc acggatcggg
     1381 gcgatcagcc tgctgccgcg cccgcgccct ccgtgacgtt catcggcagg tccgcgagca
     1441 gcggctgggt ccacgtccac gccgcgccgt ggtgcttgcc gacggccccg tggcgcgcgg
     1501 cctcgacacg cagcggcagc ccgaagatct cgatgaatcc gaccgccgat gcatggtcga
     1561 acgcgtcgcc ctcgtcgtag gtcgccaggt ccttgtcgta caggctca
//
LOCUS       DBNHXO010000433         2567 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2113763, whole genome shotgun sequence.
ACCESSION   DBNHXO010000433 DBNHXO010000000
VERSION     DBNHXO010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2113763"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1459
                     /locus_tag="AC162A_08750"
     CDS             35..1459
                     /locus_tag="AC162A_08750"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HIR5010969.1"
                     /translation="MTAVRPVTGQTAHAAPALGMRLAALVEARNASTETGVASVTASV
                     APVDAISLYAAAVEANLEAALWSRPTEGIAFVGVGRAWATEPAGPERFAEAEQAWRTL
                     LRTSRIDGASPVLMGGLGFTGREPHEGDVWAPFAPASLVLPELLYASTLDGAALTASV
                     VGAADDIDGRWAALERRARELEPSPNGIVARPVFAPLTVTDEQPGHEAWDRLVGMFAG
                     AVGRGRIDKVVLARRVGLRSPVELDVPNALRRLAASAPESTTYAFRRGGRTFLGSTPE
                     RLARTEGRAFRTMAVAGSIRRGADAAEDAALAAELLASEKDREEQAIVVDSIRDQLVP
                     IAETLEVAPEPSVMTLRYVQHLATEISGTLPEARGLLSLAGLLHPTPAVGGQPRDVAL
                     AMVDEHEGFDRGWYAGPIGWLGADGDGELCVALRCGIVDHATATLFAGCGIVADSDPE
                     AEWEESRMKLRAVISALGIPAGER"
     gene            1456..>2567
                     /gene="menD"
                     /locus_tag="AC162A_08755"
     CDS             1456..>2567
                     /gene="menD"
                     /locus_tag="AC162A_08755"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HIR5010970.1"
                     /translation="MTDAALLRAFVAEFAAAGVRDAVVCPGSRSTPLALALGVSPDIR
                     VRVLLDERAAGFFALGIARTDRRPVAVLVTSGTAAMELAPAVVEAAYSRVPLVVLTAD
                     RPAELRDRGAAQTIDQDHLYGRAAKWFAELPLFDGHPATAAHVRSVAGRAVATAWAGP
                     AGPVHLNVPFREPLLPDGSLEPADVGATPTEGRPTTATMTGRRTLDDAGLDAVAERVA
                     AARRGLIVAGPDDDPALPVALATLAGATGFPILADPLSGLRAGRHDRSMVVARGDQLV
                     RRGPWIDAHRPDLVIRTGAMPTSKPIAELLAAARPELLILDGDGGWRESALVPATFVH
                     ADPAATATALARRTDGGRAADAWAGAWLEADAAATRT"
BASE COUNT          302 a          942 c          979 g          344 t
ORIGIN      
        1 tcgcggacgg ggccgacttc ccggagaagg cccgatgacg gccgtccggc cggtcacggg
       61 gcagaccgcg cacgcggcac cggcgctcgg catgcgcttg gcggcgctgg tcgaagcccg
      121 gaacgcgtcg accgagacag gggtcgcctc cgtgaccgcg tccgtagccc ccgtcgatgc
      181 gatctcgctc tacgccgcgg cggtcgaggc gaacctcgag gccgcgcttt ggtcgcgccc
      241 gaccgaaggg atcgcgttcg tgggcgtggg ccgcgcgtgg gcgacggagc ccgcgggacc
      301 cgaacggttc gcggaggccg agcaggcctg gcgaacgctg ctgcggactt cccgaatcga
      361 cggcgcgagc ccggtgctga tgggtggcct cgggttcacg ggccgcgagc cccatgaggg
      421 tgacgtctgg gcgccgttcg cgcctgcgtc cctggtgctg cccgagctcc tctacgcaag
      481 cacgctcgat ggcgcggcac tgaccgcgtc ggtcgtcggc gcggcggacg acatcgacgg
      541 ccgatgggcg gcgctggagc ggcgcgcgcg agagctcgag ccgagcccga acggcatcgt
      601 cgcgcgcccc gtatttgcgc cgctcacggt gaccgacgag cagccggggc acgaggcgtg
      661 ggaccggctg gtgggcatgt tcgcgggcgc cgtcggtcgg ggcaggatcg acaaggtcgt
      721 cctggcgcgc cgggtgggcc tgcgctcgcc cgtcgagctc gatgtcccca atgccctccg
      781 ccgcctcgcc gcaagcgccc ccgagagcac gacctacgcg ttccgtcgcg gcggccgtac
      841 gttcctgggc tcgaccccgg agcgcctcgc tcgtaccgag ggtcgcgcgt tccggacgat
      901 ggccgtggcg ggatcgatcc gccggggcgc cgacgccgcc gaggacgccg ctcttgccgc
      961 cgagctcctg gcgtccgaga aggaccgtga ggagcaggcg atcgtcgtgg actcgatccg
     1021 ggaccagctg gtgccgatcg ccgaaaccct cgaggtcgcc ccggagccgt ccgtcatgac
     1081 gctccgctac gtccagcacc tggcgaccga gatctcgggc accctgcccg aggcgcgcgg
     1141 cctgctctcc ctcgccggcc tcctccatcc aacgcccgcc gtcggcgggc agccgcgcga
     1201 cgtcgcgctg gcaatggtcg acgagcacga gggcttcgac cggggctggt acgccgggcc
     1261 gatcggctgg ctcggcgcgg acggtgacgg cgagctgtgc gtggcgctcc ggtgcgggat
     1321 tgtcgaccac gcgacggcaa ccctgttcgc cggctgcggc atcgtcgcgg actccgaccc
     1381 ggaggccgag tgggaagaat cacggatgaa gctccgggcc gtcatttcgg ccctgggcat
     1441 cccggcgggc gagcgatgac cgacgcggcg ctcctgcgcg cgttcgtcgc cgagttcgcg
     1501 gcggccggcg tccgcgatgc cgtcgtgtgc cccgggtcgc ggtccacgcc cttggcgttg
     1561 gcgctcggcg tcagccctga catccgggtt cgggtgctcc tggacgagcg tgcggccggg
     1621 tttttcgccc tgggcatcgc gcgcaccgat cggcgccccg tcgcggtgct ggtcacgtcg
     1681 gggaccgcgg cgatggagct tgccccggcg gtcgtcgagg ccgcgtactc gcgggtaccg
     1741 ctggtggtgc tgaccgccga ccggccggcg gagctgcgag atcgaggcgc cgcccagacc
     1801 atcgaccagg accacctcta cgggcgggcc gcgaagtggt tcgcggagct gccgctgttc
     1861 gacggccacc cggcgacggc ggcccacgtc cggtcggtgg cgggccgggc cgtggcgacg
     1921 gcttgggccg gacccgccgg gccggtccat ctcaacgtcc cgttccggga gccgttgctg
     1981 cccgacgggt cgctcgagcc ggcggatgtg ggtgcgacgc cgaccgaagg tcgaccgacg
     2041 accgcgacga tgacgggtcg gcgaacgctc gatgacgcgg ggctcgatgc cgtggccgag
     2101 cgcgtggccg cggcccggcg tggcctcatc gtcgcgggcc cggacgatga cccggctctc
     2161 cccgtggccc tggcgacgct cgcgggcgcg accggcttcc cgatcctcgc cgacccgctg
     2221 tcggggctac gagccgggcg ccacgaccgc tcgatggtgg tcgcccgcgg cgaccagctg
     2281 gttcgtcggg ggccatggat cgacgcccat cgccccgacc tcgtcatccg caccggcgcg
     2341 atgccgacgt ccaagcccat cgccgagctc ctcgcggctg cgaggccgga gctgctgatc
     2401 ctcgacggcg acggaggctg gcgcgagtca gcgctcgtgc ccgcgacctt cgtccacgcc
     2461 gatcccgccg ccaccgcgac cgcgctcgcg agacgaaccg acggcgggcg agccgccgac
     2521 gcctgggcgg gcgcgtggct cgaggcggac gcggcggcga ccaggac
//
LOCUS       DBNHXO010000434         4744 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5092031, whole genome shotgun sequence.
ACCESSION   DBNHXO010000434 DBNHXO010000000
VERSION     DBNHXO010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5092031"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1648
                     /locus_tag="AC162A_08760"
     CDS             230..1648
                     /locus_tag="AC162A_08760"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HIR5010971.1"
                     /translation="MAVSAFDLFKIGIGPSSSHTVGPMRAARQFALRLEHQALIERTA
                     RVRTGLYGSLGATGRGHGTDKAILLGLEGEQPDTVDIDAIPGRLDTIRSTGRLRLLGR
                     HVVAFDEPTDLVFSQQVSPDDTTPFHTNNIRFAALGGGGEVLAEGTYYSVGGGFVVSG
                     ELAADGSAPHGVIPAIDVLPFPYHSADELLAIAAAENTTIAGIVRRNERHWRTDDEID
                     AGLLRIWAAMQACIERGLRVGGTLPGPLAVRRRAAALHRSLTAGGSVPGDEAAGGDAL
                     AVLDWVNVYALAVNEENAAGGRVVTAPTNGAAGIIPAVLAYYARHLPGSTDAGVIEFL
                     LTASAIGVLYKENASISGAEVGCQGEVGVACSMAAAALCAVLGGTPAQVENAAEIGIE
                     HHLGLTCDPVGGLVQIPCIERNAIGSVKAINAARMALAGDGSHHVSLDAAIKTMRETG
                     ADMTTKYKETSRGGLALNVVEC"
     gene            complement(1693..2394)
                     /locus_tag="AC162A_08765"
     CDS             complement(1693..2394)
                     /locus_tag="AC162A_08765"
                     /inference="COORDINATES: protein motif:HMM:NF018590.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HIR5010972.1"
                     /translation="MTDEKKNNEITDPATGKVQGFVVAGEQDGITSVVGAAADDQGNA
                     IIEGVVADDQGILAEGAIGISGDNAILVARFANMDAAKAAYENLIEAEKSGALDIDGV
                     LVANADAEGKINVVKMTDHHTKKGFLAGAVAGIVVGIIFPPSIIASALAVGVGGAAIG
                     KLGNLNAKSQTARELADVLTPNSSGIIALVQLAQVEEVKEQLPEAAEVKAAPVSDEAA
                     AAVKSAAEAAGSVPA"
     gene            2580..3425
                     /locus_tag="AC162A_08770"
     CDS             2580..3425
                     /locus_tag="AC162A_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012730.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR5010973.1"
                     /translation="MTAQSPTLREHGNAYNIFILVLTIFSLAIMVLLLLPVDDQTRQL
                     LNVYDNAVAFIFLADFTYNITGARPKSGYFIHQRGWLDLLGSIPTFGLFQLAALLRLA
                     RLSRLARIGRLMGGQKRKDLIADVLENRGQYATFITILAAGIVLCTASILVLQFESTD
                     PAANITTGGDALWWAVVTITTVGYGDKYPITTLGRLTGVSVMFAGVGIIGALASILAS
                     LLVPPPKPPEEPTEAEIEEAAATAAASAATSAAAPATDAVAAELANLRAEIAALREAL
                     PPKPG"
     gene            complement(3515..3919)
                     /locus_tag="AC162A_08775"
     CDS             complement(3515..3919)
                     /locus_tag="AC162A_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010974.1"
                     /translation="MKNPPILISVLGFFGLMAGLYYIYAGLRIWGFNFFGMFDTADVT
                     AGWGFWGLMWIVVGLIYFAASWALWSLQPWAWLFAVILSVFALISAFFVMFDSGLGPA
                     LGAAILPGIILLYLNSAEVRRAFDVDGTAGGV"
     gene            complement(4048..>4744)
                     /locus_tag="AC162A_08780"
     CDS             complement(4048..>4744)
                     /locus_tag="AC162A_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR5010975.1"
                     /translation="GAVVIASVLGLIDLEEIRRYWRWRRTDAILAVVALVGVATTDIL
                     AGLVIAVLLSLMMLLYRASRPYLAVVGRLPGARAVYADVARHEAAEAVPGLLLVRLDA
                     PLYFFNANVARAQILALVAGTDPPPHGILIDLAATADLDVTSADMLYELVGDLEERSI
                     EVLLAQVKGSVRDRMRKTGLMDRIGEDRLYLSLGSAVTDFGRRWPSEPPPAPVEPSVE
                     PPVEPSEPMPGPS"
BASE COUNT          721 a         1714 c         1632 g          677 t
ORIGIN      
        1 gcaacgcgga ggccagcgtg gtcgcctcgg tcatcggaac ccagggcgcc gtcgtatccg
       61 gcggcttgct gacgctggcc gggctgggtg cgatcgccct cgcgatgccg gagttccgtc
      121 ggctcgacat gccaaccgcc atcgccgaca ccgccgcggc ggcgcacgcg ggcggctgat
      181 cgcgccccgg ccggggtgac aatggggccg gccataagag gagtcacggt tggcggtcag
      241 cgcgttcgac ctgttcaaga tcggcatcgg gccgtcgagc tcgcacaccg tcggcccgat
      301 gcgggcggcg cgccagttcg cgctgcgcct cgagcaccag gcgctcattg aacgcacagc
      361 ccgcgtcagg accgggctct acggctcgct cggcgccacg ggccgcggcc acggcaccga
      421 caaggcgatc ctgctgggcc tcgagggcga gcagccggac acggtcgaca tcgacgcgat
      481 cccggggcgc ctcgacacga tccgctcgac ggggcggctc cgcctcctgg gccgccacgt
      541 ggtcgcattc gacgaaccca ccgacctcgt gttcagccag caggtcagcc cggacgacac
      601 aacgccgttc cacaccaaca acatccggtt cgcagcgctc ggtggtggtg gcgaagtcct
      661 cgccgagggg acctactact cggtcggcgg cggcttcgtg gtcagcggcg agctggcggc
      721 cgacgggagc gcgccgcacg gcgtgatccc cgcgatcgac gtgctgccgt tcccgtacca
      781 cagcgcggac gagctcctcg ccatcgcggc cgcggagaac acgaccatcg ccgggatcgt
      841 ccgccgcaac gagcgccact ggcgcaccga cgatgagatc gacgccggcc tgctccggat
      901 ctgggcggcg atgcaggcgt gcatcgagcg cggccttcgg gtgggcggga cgttgccggg
      961 gccgctcgcg gttcggcggc gcgcggccgc gctccatcgc tcgctgacgg cgggcggatc
     1021 cgttcccggc gacgaggcgg ccggcggcga tgcgctcgcg gtgctggact gggtcaacgt
     1081 ctacgcgctg gcggtcaacg aggagaacgc ggccggcggg cgggtggtca ccgcgcccac
     1141 gaacggcgcc gccgggatca tcccggccgt gctcgcctac tacgcgcgcc acctgcccgg
     1201 ctcgaccgat gccggcgtca tcgagttcct gctgaccgcg tcggcgatcg gcgtgctgta
     1261 caaggagaac gcctcgatct ccggcgccga ggtcggctgc cagggcgagg tcggggtcgc
     1321 ctgctcgatg gcggcggccg cgctgtgcgc cgtgctcggc ggcacgcccg cccaggtcga
     1381 gaacgcggcc gagatcggga tcgagcacca cctgggcctg acctgcgacc cggtgggcgg
     1441 gctggtccag atcccgtgca tcgagcgcaa tgcgatcggg agcgtgaagg cgatcaacgc
     1501 cgcccggatg gccctcgcgg gcgacgggtc acatcacgtc tcgctcgacg ccgcgatcaa
     1561 gacgatgcgc gagacgggcg ccgacatgac gaccaagtac aaggaaacgt cgcggggcgg
     1621 cctcgccctc aacgtcgtgg agtgctaggg cgggccgacg ggacgatcgt ccgtccaccc
     1681 gccccacgcg gttcaggccg ggacgctgcc cgcggcctcg gcggcgctct tgacagcagc
     1741 cgccgcctcg tcgctgaccg gggccgcctt gacctcggcc gcctcgggca gctgctcctt
     1801 gacctcctcg acctgcgcca gctggacgag cgcgatgatc cccgacgagt tcggggtcag
     1861 cacgtccgcg agctcgcggg ccgtctggct cttggcgttg aggttgccga gcttcccgat
     1921 cgctgcgccg ccgacgccca cggcgagcgc tgaggcgatg atcgagggcg ggaagatgat
     1981 gcccacgaca atgcccgcga cggccccggc gaggaagccc ttcttggtgt ggtggtcggt
     2041 catcttgacg acgttgatct tgccctcggc atcggcgttg gccacgagga ccccgtcgat
     2101 atcaagcgcg ccggacttct cggcttcgat cagattctcg taggcggcct tcgcggcgtc
     2161 catgttcgcg aatcgcgcga ccaggatcgc gttgtcgccg gagatgccga ttgcgccctc
     2221 ggcgaggatg ccctggtcgt cggcaacgac gccttcgatg atggcgttcc cctggtcgtc
     2281 ggccgcggcc ccgaccacgc tcgtgatccc gtcctgctcg cccgcgacga cgaatccctg
     2341 gaccttgccg gtcgccgggt cggtgatctc gttgttcttc ttctcgtccg tcatctgtcc
     2401 ccgctccatg agcttccccg ggtctccgat cgtgtgcgat ctgtgcggcc gcggactccc
     2461 gagtctaccg ggcggctcca accgcaggct caccgttcct tggtgaattc cgggcgggcc
     2521 gaggtcgcta ccatgatcgc ggcccaggcg ggtccaggtt cgaggaggag cgacgccccg
     2581 tgactgccca gtcgccgacg ctgcgcgagc acggcaacgc ctacaacatc ttcatcctgg
     2641 tcctgaccat cttctcgctc gcgatcatgg tcctgctcct gttgcccgtc gacgatcaga
     2701 cgcgtcaact cctgaacgtc tacgacaacg cagtcgcgtt catcttcctc gcggacttca
     2761 cctacaacat cacgggcgcc cgccccaagt ccgggtactt catccaccag cgcggctggc
     2821 tggacctgct cggctcgatc ccgaccttcg gcctgttcca gctcgcggcg ctcctccgcc
     2881 ttgcccggct gagccggctt gcccgcatcg gacgcctcat gggcggccag aagcgcaagg
     2941 acctgatcgc ggatgtgctc gagaaccgcg ggcagtacgc gacgttcatc acgatcctcg
     3001 cggcgggcat cgtcctctgt acggccagca tcctggtgct gcagttcgag agcacggatc
     3061 ccgccgccaa catcacgacg ggcggcgatg ccctgtggtg ggccgtggtg acgatcacca
     3121 ccgtcggcta cggtgacaag tacccgatca cgaccctcgg ccgcctgacg ggcgtgtcgg
     3181 tgatgttcgc gggcgtcggg atcatcggcg ccctggccag catcctcgcg agcctcctcg
     3241 tcccaccgcc gaagccgccc gaggagccga ccgaggccga gatcgaggag gccgccgcga
     3301 ccgcggctgc gtctgcggcc acgtccgcgg cggcgccagc aaccgatgcg gtcgcggccg
     3361 agctcgccaa cctgcgtgcc gagatcgccg ccctgcgcga ggcgctcccg cccaagccag
     3421 gctgatcgct cgtcgggcgc gaaacgggcg cccgcggcgg acgcccgtgt gcggcgagtc
     3481 gggccggtga caccggcccg gaatgggttc ggcgctagac gccgccggcg gtcccgtcga
     3541 cgtcgaaggc cctcctgacc tcggccgagt tcaggtagag caggatgatg cccggcagga
     3601 tcgccgcacc gagcgcgggt ccgaggccgg aatcgaacat cacgaagaac gccgagatga
     3661 gggcgaacac ggacaggatc accgcaaaca gccacgccca cggctgaagc gaccacagcg
     3721 cccaagaggc cgcgaagtag atcagcccga cgacgatcca catcaggccc cagaagcccc
     3781 agcccgcggt gacgtccgcc gtgtcgaaca tcccgaagaa gttgaacccc cagatccgca
     3841 ggcctgcgta gatgtagtac aacccggcca tcaggccgaa gaagccgagc accgagatca
     3901 ggatcggcgg attcttcacg actggctcta cctcactccg acgctcgcga cgccccggcg
     3961 ctcatggatc gctcggacgg cagacccgcg agtcgccgaa gcgtacgccc ccgccggaac
     4021 ggcccgcaag gcgaactggc gtgcccgtca gctcgggccg ggcataggct cggacggttc
     4081 gaccggcggc tcgaccgacg gctcgaccgg cgcgggcggc ggctcgctgg gccagcgacg
     4141 cccgaagtcg gtcacggcag atcccagcga gaggtacagg cggtcctcgc cgatccggtc
     4201 catcaagccg gtcttcctca tccggtcgcg cacggagcct ttgacctggg ccaggagcac
     4261 ctcgatcgag cgctcctcga ggtcgccgac gagctcatac agcatgtcgg ccgacgtgac
     4321 atcgaggtcc gccgtggccg cgaggtcgat caggatcccg tgcggtggcg ggtcggtgcc
     4381 agcgacgagc gccaggatct gggcgcgggc cacattcgcg ttgaagaagt agagcggcgc
     4441 gtcgaggcgc accagcagga ggccgggcac ggcctccgcg gcctcgtggc gggcgacgtc
     4501 cgcatagacg gcgcgcgctc cgggcagcct gcccaccacg gccaggtacg gccggctggc
     4561 gcggtacagc agcatcatca gggacagcag cacggcgatc acgaggcccg ccaggatgtc
     4621 cgtggtcgcg acgccgacca gcgccacgac cgccaggatg gcatccgtgc ggcgccaccg
     4681 ccagtagcgc cggatctcct cgaggtcgat gagccccagc acggacgcga tgacgacggc
     4741 accc
//
LOCUS       DBNHXO010000435         5692 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1522419, whole genome shotgun sequence.
ACCESSION   DBNHXO010000435 DBNHXO010000000
VERSION     DBNHXO010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5692)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1522419"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..800
                     /locus_tag="AC162A_08785"
     CDS             150..800
                     /locus_tag="AC162A_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010976.1"
                     /translation="MATTPLARIAGPIAIVAGLLVLVTRLIVMLTTPAVLGDELKASV
                     LSPTFAVNSVLSIIAFTTLVLALFAAYEWQARAAGRFGLVAVVAALVGTMFMTGDWWY
                     EAFAVPWLADVAPAVFQTGADGRLLIGGLTSFALFSLGWALFGAASLRARVFPRAVSW
                     AILVGGVLAGIPIAGAYLYGSLLFGLAIASLGVWLVRTSSRTVPEARPLEAIAPAG"
     gene            1089..1733
                     /locus_tag="AC162A_08790"
     CDS             1089..1733
                     /locus_tag="AC162A_08790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR5010977.1"
                     /translation="MLALGPAVIAAAIAWQEGNGSLRTLAHSLTIRPADRRWYLVLAI
                     PVLWSLGTVAVAVGLGEPVAGLFDTVFPAILIIPLVVLLPAFAEELAWRGFALPRLMS
                     AMSPLAAALVLAIPWTLIHVFLFLPGQWYGDLAVWPMAVSIFSYSILLTWIFVGTGGS
                     VLMTGLFHAALNGVAPIMAGVDADRSWVIRNLLAAGIAVAVVALGGLRRQTPAR"
     gene            1793..2524
                     /locus_tag="AC162A_08795"
     CDS             1793..2524
                     /locus_tag="AC162A_08795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR5010978.1"
                     /translation="MTLPEQLSTPRLLLLFLVPGALMTLVFVLLAPVVERLGFPPITA
                     LLLAIVGVLIPFELAVVLRAGRAAGSVHAAIPYRVPMPLRTWLWLVPVLILAAFLGFG
                     IHQLIEPWLIGTFFGWLPEWYVTPISMDHIADYSRTVWTVTLVAFLAINGFVGPIVEE
                     LYFRGYLLPRMERMGRWAPLVNVSLFSLYHFWSPWQLVARILGFAPTVYAVRWKRNIY
                     LGMVVHCTLNTIAVLLVASLIFGRL"
     gene            complement(2639..2992)
                     /locus_tag="AC162A_08800"
     CDS             complement(2639..2992)
                     /locus_tag="AC162A_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010979.1"
                     /translation="MLERAEIWARVRTPDNLFGWVPCMTLEAGSADPAEDPRAAIDLE
                     LPAVVEEPIALEALLEAVAAQRLAIREPPLEAAPAPPRRRTRKPKAEAPPAETPPKST
                     RRRPQRPKAMPDQEA"
     gene            complement(3654..5039)
                     /locus_tag="AC162A_08805"
     CDS             complement(3654..5039)
                     /locus_tag="AC162A_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
                     /protein_id="HIR5010980.1"
                     /translation="MANDGDVLTRLADGAKAVLDANWLGASTLPSRTLYPHQWNWDSA
                     FIAIGRSWYDEPRAQQELRSLFRAQWADGRVPHIVFNPSVGEDAYFPGPAFWQSSKRS
                     ASAPRDVETSGITQPPVHARAALEMHRHAHDVDASKAFLAWLYPRLVAEHDYLALRRR
                     PTGTDLPVIMHPWESGLDNSPVWDRDLAQMVIPPGSIPPYVRHDLDHAKAADRPTNAA
                     YDQFVFLAARYRDSGYDDDQLLEQVPFLVAGPLFNAIHCWSTYALAEIAEIVGADPVP
                     HREDARRISAALISELWDPATRRFCALDIVRNERSVEDTIVSFAPLLDPALPREQLDA
                     IMADLRSASFHPDRPDGFVAPSYDLLGEGFDERRYWRGPVWINTNWLLWQGLRQHGQT
                     AEADEILLSSLRLVDRSGFHEYFDPFGGAAFGTDGFGWTAALTLDLVERHQGADRARL
                     LERLAEAATGA"
     gene            complement(5042..>5692)
                     /locus_tag="AC162A_08810"
     CDS             complement(5042..>5692)
                     /locus_tag="AC162A_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017717520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
                     /protein_id="HIR5010981.1"
                     /translation="RPGGSSMILSLLPPIHLERVLRDALSEEAFLSPHGMRALSRRHL
                     GDPFRIEIEGYTASIDYEPGESTNNLFGGNSNWRGPVWFPVNYLFIESMLRWDEALGA
                     DFTVEYPTGSGVRLRLRDVAADLSARLVSIWLPDADGHRPVAGSIAKFRDDPEWRDLL
                     LFHEYFHGDTGAGIGASHQTGWTGLVAHLLCRGGPMGGAERPIIAPAAAEAIGAVT"
BASE COUNT          741 a         1981 c         2025 g          945 t
ORIGIN      
        1 cgctcgctga ctgaccgctc cgggcaggcc ctgccccgcg agccgggacc gccccgatgt
       61 gtacgccccg atctccggca acgcttggtc ggcagtgcag tcccgccagt ggctgggccc
      121 gctcgcacgc cgatgaaggg agcttgaaca tggccaccac accgctcgcc cggatcgcgg
      181 gcccaatcgc catcgtcgcc gggcttctcg tgctcgtgac gcgcctgatc gtgatgctca
      241 ccacgcccgc cgtgctgggc gacgagctca aggcgtcggt cctcagcccg acgttcgccg
      301 tcaacagcgt gctatcgatc atcgccttca cgacgctcgt gctggcgctg ttcgcggcct
      361 acgagtggca ggcgagggcg gccggcaggt tcggccttgt cgcggtggtc gccgcgctcg
      421 tggggaccat gttcatgacc ggcgactggt ggtacgaggc cttcgcggtc ccgtggttgg
      481 cggacgtcgc acccgccgtg ttccagaccg gcgccgacgg ccgattgctc atcggggggc
      541 tgacgagctt tgccctgttc tcgctcggat gggccctgtt cggcgcggcc agccttcggg
      601 cgcgagtctt cccacgggcc gtctcctggg cgatcctggt cggtggcgtg ctcgcgggca
      661 ttcccatcgc cggcgcctat ctgtacggca gcctgctgtt cgggctcgcg atcgcctcgc
      721 tgggcgtctg gctcgtcagg acgtcatcgc ggacagttcc cgaggcccga cctctggagg
      781 cgattgcccc ggcaggctga tccgcagctt gaccggcgtc gagcggccgc cccgaggggg
      841 ccggtcaagg gcgctggagg ctggacacgg cgtcggacaa tcgggcggac atggcagatc
      901 aggaggacct ggcgatgacg acttcgcgag tcccgacgcg ctcacaggcg gtgaccgaac
      961 gccgaccgtc cagcggcgaa cgctcggtcc gccggcagct tgggagcgtc gcctgtttcg
     1021 ttgggctaag cctcggcctg ggggtggtgg cgatcgtggc tcggacccct ccggccacgg
     1081 ttccctttgt gctggcgctc gggcccgccg tgatcgccgc cgcgatcgcc tggcaggagg
     1141 gcaacgggtc cctccgcacc ctcgcccact cgctgacgat ccggccggcg gaccgccgct
     1201 ggtatctcgt cctcgccatt ccggtgctgt ggtcgctcgg gaccgtggcc gtggccgtcg
     1261 ggctcggcga gcccgtcgcg ggattgtttg acacggtgtt cccggccatc ctgatcatcc
     1321 cgctcgtcgt gctgctgccc gccttcgccg aggagctcgc gtggcgcggc ttcgcgctcc
     1381 cgcgactgat gtctgcgatg tcgccgctcg ccgccgccct cgtgctcgcg atcccgtgga
     1441 ccctgatcca tgtcttcctg ttcctgccgg gccagtggta cggcgatctg gccgtgtggc
     1501 cgatggccgt gagcatcttc tcgtactcga tcctgctgac gtggatcttc gtcgggaccg
     1561 gtgggagcgt cctgatgacc ggcctgttcc acgcggcgct caacggcgtg gccccgatca
     1621 tggccggggt cgacgcggac cgctcgtggg tcatccggaa cctgctcgcc gcggggatcg
     1681 cggttgccgt cgtcgccctc ggtggcctgc ggcgccagac ccctgcacgg tgaacgttcc
     1741 caattcggta gcgacggagg tcgccgttcc caccacaatt gacgccgcca ccatgacgct
     1801 ccccgagcag ctgtcgacac ccaggctgtt gctgctgttc ctcgtcccgg gcgcgctgat
     1861 gacgctggtg ttcgtgctgc tcgccccggt cgtcgagcgc ctggggttcc cgccgatcac
     1921 cgccctgctc ctcgcgatcg tcggcgtcct catcccgttc gagcttgcgg ttgtcctgcg
     1981 ggccggccgc gcggccggct cggtgcacgc ggcgatcccc tatcgggtgc cgatgccgct
     2041 ccgcacgtgg ctgtggctcg tgcccgtcct gatcctggcg gcgttcctgg gcttcggtat
     2101 ccaccagctg atcgagccct ggctcatcgg cacgttcttc ggctggctgc ccgagtggta
     2161 cgtgacgccg atctcgatgg accacatcgc cgattactcg cgcacggtgt ggaccgtgac
     2221 cctggtcgcg ttcctcgcga tcaacgggtt cgtcggtccg atcgtggagg agctgtactt
     2281 ccgcggctac ctgctgccgc ggatggagcg catgggccgc tgggcgccgc tcgtcaacgt
     2341 cagcctgttc tcgctgtacc acttctggtc gccgtggcag ctggtcgcgc ggatcctcgg
     2401 cttcgcgccg acggtgtacg cggttcgctg gaagcggaat atctacctcg ggatggtcgt
     2461 ccactgcacg ctgaacacga tcgccgtcct gctcgttgcc agcctcatct tcgggcggct
     2521 ctgagcgcgg ttcgcgtcgg cgctgagctc tcgctcggcg accaaccgat cgatggtcac
     2581 gatgaccgac ggtcgcgccc gatgctcagg tcccgtggac gtggaccgtc gcggtgcgct
     2641 aggcctcttg gtcgggcatc gccttcggcc gttgtggccg tcggcgcgtc gatttcggcg
     2701 gcgtttcggc gggcggtgcc tcggctttgg gcttccgtgt ccgccgtcgc ggcggcgccg
     2761 gcgctgcctc cagcggtggt tcccggatcg ccaaccgctg tgccgcgacg gcttccaata
     2821 gcgcctcaag cgcgatcggt tcctcgacca cggcggggag ctcgaggtcg atcgccgcgc
     2881 gaggatcctc agccggatcg gccgacccgg cctcgagcgt catgcagggg acccagccga
     2941 acaggttgtc tggcgtgcgg acacgggccc agatctcggc ccgctcgagc acgttgacct
     3001 cgtctccccc gtccagctcc tggatcggcc ggcccagagc gtcgtcggga cgatcgagga
     3061 gtggcacgcc gtcatagcga acgtgatgca gcccgccgag ctcgtcgccg ggcgtgacaa
     3121 agacgatcgg aacgcgagct ggcgccgtga ccaaacggcg atccggcgcg gctgctggcg
     3181 accggtccgg cctgcgagcg gccaccagag tagggtcgag ccagcgaggt agcgtgcgat
     3241 cgcggccccg cgagttgacg gccgattgtg ggcctgcggc ggactcgtcg atctcggacg
     3301 acccccgggc cgacagacga cggcgcccga gcatgagctg cacgagcccg gcaacgccac
     3361 tcatcggtcc ctggggcccg gtcgggtcat cgccacgtcg acgcgtgccc aggcgcttgc
     3421 agagcgagca acgttcccag cctccagagc ctcgtcctca tgggtagccg catcactagg
     3481 atcacccgcc gggccgggcg gcgtcattgg cccttccgtc agcggtcggc cacccaaggg
     3541 tccaccgaca gtcccggccg cttcgctgcg ggagctcgtc gcggcgcttg gtggccgcga
     3601 gcagtaccgc gtgaccggca ctctcggcgc gctccgtaca cggggagcgg agcctaggct
     3661 ccggtcgcgg cctcggccag gcgctcgagg aggcgcgccc gatccgcgcc ctggtggcgc
     3721 tcgacgaggt cgagcgtcag ggccgccgtc cagccgaagc cgtcggtgcc gaacgcggcg
     3781 ccgccgaacg ggtcgaagta ctcgtggaag ccggaccggt cgaccaggcg caggctgctc
     3841 agcaggatct cgtcggcctc tgcggtctgg ccgtgctggc ggaggccctg ccacagcagc
     3901 cagttggtgt tgatccagac cgggccgcgc cagtagcgtc gctcatcgaa gccctcgcca
     3961 aggaggtcgt agctcggggc gacgaagccg tcgggccggt cggggtggaa gctcgccgag
     4021 cggaggtcgg ccatgatcgc atccagctgc tcgcgcggaa gcgccggatc gaggagcggc
     4081 gcgaacgaca cgatcgtgtc ctcgacgctg cgctcgttgc gcacgatgtc aagggcgcag
     4141 aaccgcctgg tcgccgggtc ccagagttcg ctgatgagcg cggcgctgat ccgccgcgcg
     4201 tcctctcgat gcgggaccgg gtccgccccg acgatctcgg cgatctcggc cagcgcatac
     4261 gtcgaccagc agtggatggc gttgaacagc ggtcccgcga cgaggaacgg gacctgttcc
     4321 aggagctggt cgtcgtcgta gccggagtcg cggtagcggg cagccaagaa cacgaactgg
     4381 tcgtacgccg cgttggtcgg ccggtcggcc gccttggcat ggtcgaggtc gtggcggacg
     4441 tagggcggga tcgagcccgg cgggatcacc atctgcgcga gatcgcggtc ccagactggc
     4501 gagttgtcga ggcccgactc ccacgggtgc atgatcacgg gcaggtcggt gccggtgggg
     4561 cggcggcgca gggcgagata gtcgtgctcg gcgacgaggc gcggatacag ccaagcgagg
     4621 aaggccttcg aggcgtcgac gtcgtgcgcg tggcgatgca tctccagcgc ggcgcgggcg
     4681 tggacgggcg gctgcgtgat gccagatgtc tccacgtcac gcggcgcgct cgccgaccgc
     4741 ttagacgact gccagaacgc gggccccggg aagtaggcgt cctcccccac cgaggggttg
     4801 aacacgatgt gcggcacccg cccgtcggcc cactgcgctc ggaacaggga ccgcagctcc
     4861 tgctgggccc ggggctcgtc gtaccacgag cgaccgatcg cgatgaacgc cgagtcccag
     4921 ttccactggt gcggatacag cgttcgggac gggagcgtgg acgcgcccag ccagttcgcg
     4981 tcgaggacgg ccttcgctcc gtcggccagc cgtgtcagga catcgccgtc gttcgccatc
     5041 gtcaggtgac cgccccgatt gcctcggcgg cggcgggcgc gatgatcggc cgctcggccc
     5101 cgcccatggg cccgccgcgg cacaggaggt gcgcgacgag tcccgtccag ccggtctggt
     5161 gcgaggcgcc gatgccggcg cccgtgtccc cgtggaagta ctcgtggaac agcagcaggt
     5221 cgcgccactc ggggtcgtcc cggaacttcg cgatcgagcc cgcgaccggg cggtgaccgt
     5281 cggcgtccgg gagccagatc gagaccaggc gggccgagag atcggcggcc acgtcgcgca
     5341 ggcgcagacg cacgcctgag cccgtcgggt actcgacggt gaagtcggcg cccagcgcct
     5401 cgtcccagcg cagcatcgat tcgatgaaca ggtagttgac cgggaaccat acggggccgc
     5461 gccagttgga gttgcccccg aacaggttgt tggtcgactc tccgggctcg tagtcgatgg
     5521 acgccgtata gccctcgatc tcgatccgga acggatcacc gagatggcgg cgcgacaatg
     5581 cccgcatgcc gtgcggcgag aggaatgcct cctcggacag cgcatcgcgc agcacccgct
     5641 cgaggtggat cggcggcagg aggctgagga tcatcgacga cccgccgggc cg
//
LOCUS       DBNHXO010000436         2064 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_926493, whole genome shotgun sequence.
ACCESSION   DBNHXO010000436 DBNHXO010000000
VERSION     DBNHXO010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_926493"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="AC162A_08815"
     CDS             complement(<1..629)
                     /locus_tag="AC162A_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5010982.1"
                     /translation="MSPRSAASADRLESAWAQRVRENRDQAERFRETQTPDFYAPVSS
                     LFVADPRRTDEPALVALLASATDEQVWLDIGAGAGRYALPLALVVREVIAVEPSAGMR
                     GALRTGMGEHGISNVRVVAGAWPESLERLGDLPAADVSMIAHVGYDIEAIGPFLDAME
                     AATRDHCIAVLTDRSPASVADPFWPLVHGEERVPLPALPDLLEVLHSRGR"
     gene            complement(636..1220)
                     /locus_tag="AC162A_08820"
     CDS             complement(636..1220)
                     /locus_tag="AC162A_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR5010983.1"
                     /translation="MNVGAVVLAAGAGSRFGGAKLRAVLDGRPMVRHVIDAAVAAGLE
                     PIVVVVPPGDALDGVDLAPARSVTNPMPAEGLSSSVRLGLRALEGEAVEAAVIMPGDQ
                     PRVRVAVIAALIAAAEQRPGTPFMVPQYRDDAAPNPILARRSTWRLADELAGDRGFGP
                     VLDAHPELVGRVPVDGANPDVDTVADLLRLQGVS"
     gene            complement(1217..>2064)
                     /locus_tag="AC162A_08825"
     CDS             complement(1217..>2064)
                     /locus_tag="AC162A_08825"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR5010984.1"
                     /translation="LISPRVPEAAITAARETREGRGDGRAIVTPLPADAPPAAFGAAQ
                     PGPGAEPPEVLVVHEDGALEGSLGDAAADAALVEAARDALAHGVSRTIEVAGRQLFVE
                     AYPVRPRLVVVGAVEVARTLVRLAKELGYETVVIDGRAAFATEARFPDVDRLLVGWPD
                     ELADEIGLGPADAVAVLTHDVKFDEPAIVEALRRGCRYVGAVGSRKTQADRRQRLLAA
                     GVAADDLDRLRGPIGLDLGGRLPSETALAIMAEVVAERRAGTGQPMRELARAREAQAA
                     AGAAG"
BASE COUNT          270 a          794 c          759 g          241 t
ORIGIN      
        1 cgaccgcgcg agtggaggac ctcgagcagg tccggcagcg ccggcagcgg cacccgctcc
       61 tcaccgtgca ccagtggcca gaacgggtcg gcgaccgacg ccgggctgcg gtcggtcagc
      121 acggcgatgc agtggtcccg ggtggccgcc tccatcgcgt cgaggaaggg gccgatggcc
      181 tcgatgtcgt agccgacgtg cgcgatcatc gagacgtcgg cggcgggcag gtcgcccagc
      241 cgctcgagcg actcgggcca ggcgccggcg acgacgcgga cgttcgagat gccgtgctcc
      301 cccatccccg tccggagggc gccgcgcatg ccggccgacg gttcgacggc gatcacctct
      361 cgcacgacca gcgcgagcgg cagcgcgtag cggcccgcgc cggccccgat gtccagccag
      421 acctgctcgt cggtcgccga tgcgagcagc gcaaccaggg ccggctcgtc ggtccgccgc
      481 ggatccgcga cgaacaacga cgataccggc gcatagaagt cgggggtctg ggtctcgcgg
      541 aaccgctccg cctggtctcg gttctcgcgg acgcgctggg cccacgcgga ctcgaggcga
      601 tcggcggacg cggcgctcct aggcgacatc gcgggtcacg atacgccctg gaggcgaagg
      661 aggtccgcga ccgtgtcgac gtcggggttg gcgccgtcga ccgggacgcg gccgacgagc
      721 tcgggatgcg cgtcaaggac ggggccgaag ccgcgatcgc cggccagctc gtccgcgaga
      781 cgccaggtcg agcggcgcgc gaggatcggg ttcggcgcgg cgtcgtcgcg gtactgcggg
      841 accatgaacg gcgttccggg ccgctgttcc gccgcggcga tcagcgccgc gatgacggcg
      901 acccggacgc gcggctgatc gccgggcatg atgacggcgg cctcgacggc ctcgccctcg
      961 agcgcgcgca ggccgaggcg caccgagctc gacaggccct ccgcgggcat gggatttgtg
     1021 acgctgcgcg ccggggcgag gtcgacgcca tcgagtgcgt caccgggcgg aaccacgacc
     1081 acgatcggct cgaggccggc cgcgacggcg gcatcgatca cgtggcggac catgggccgg
     1141 ccgtcgagca ccgcccgcag cttggcgccg ccgaaccggg agcctgcgcc tgccgcgagc
     1201 acgacggccc ccacgttcat ccggccgccc cggccgccgc ctgggcctcc ctggcccgcg
     1261 ccagctcccg catcggctgt cccgtgcccg cccttcgttc cgcgacgacc tcggccatga
     1321 tcgcgagtgc cgtctcggac ggcagacgcc cgccgaggtc gagcccgatc gggcctcgga
     1381 ggcgatcgag gtcatcggcg gccacccccg ccgccagcag ccgctgccgc cggtcggcct
     1441 gggtcttgcg cgacccgacg gcgcccacgt agcggcagcc acgccggagc gcctcgacga
     1501 tcgccggctc gtcgaacttc acgtcgtggg tcaggacggc gaccgcgtcg gccggcccga
     1561 ggccgatctc gtccgcgagc tcgtccggcc agccgaccag cagtcggtcg acgtcgggga
     1621 accgggcttc ggtcgcgaac gccgcgcgtc cgtcgatgac gacggtctcg tagcccagct
     1681 cctttgcgag gcgaacgagc gtccgggcaa cctcgacggc tccgaccacc accaggcgcg
     1741 gccgcaccgg gtaggcctcc acgaacagct ggcgcccggc cacctcgatc gtgcgtgaca
     1801 ccccgtgcgc cagggcatcc cgcgccgcct cgaccagcgc cgcatcggcg gccgcgtccc
     1861 ccagcgaccc ctccagcgcg ccgtcctcgt ggacgaccag gacctcgggc ggttcggcgc
     1921 ccggcccggg ctgcgcggct ccgaacgcgg ccggcggcgc gtccgcgggc agcggcgtca
     1981 cgatcgcccg cccgtcgccc cggccttccc gggtttcgcg ggcagccgta atggccgctt
     2041 ctggcacgcg gggcgagatc agca
//
LOCUS       DBNHXO010000437         1977 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3505733, whole genome shotgun sequence.
ACCESSION   DBNHXO010000437 DBNHXO010000000
VERSION     DBNHXO010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3505733"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="AC162A_08830"
     CDS             <1..590
                     /locus_tag="AC162A_08830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5010985.1"
                     /translation="VIGIVAKAVWDTPKARLVDGYRQHIRPNHLDVGPGTGWFIGHSG
                     LAEGSRVTILDPNPHVLAHASRELRRFEVTAVQADVLKPLPVEGPFDSAGFHMVIHCL
                     PGPMTRKARAMENIAAVLAPNGVLFGATILGRSGNHSWLARRVLTAFNRQGGIDNLDD
                     SEAGLREILEASFEHVEIEVVGSAAIFAATSPRTA"
     gene            590..1282
                     /locus_tag="AC162A_08835"
     CDS             590..1282
                     /locus_tag="AC162A_08835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5010986.1"
                     /translation="MERRAWLDERRLVTRTVYDADAPTYDLDEYQGTLHGEFVARLLD
                     TCPPGGVVLDAPCGTGHYFAQVTESGRRVVGIDQSAGMLAQARARGFAEALDQVGLQD
                     LVFAGSFDAVMCIDAMENVPPEDWPLVLANLHRAVRPGGHVYLTIEEQAQALLDSAFE
                     AQAARGLPVVRGELIEGEAFAGYHYYPDRGQVLAWIDGAGFQLIEEASEAGPDGTWGY
                     RHFLLRDVAVTG"
     gene            complement(1272..>1977)
                     /locus_tag="AC162A_08840"
     CDS             complement(1272..>1977)
                     /locus_tag="AC162A_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010987.1"
                     /translation="RVGMGRGFDLVARLTVVMVVAAALLVVPGAGGGASIARAANCPS
                     APVTAAKLAGIDGATGLACYGGQLLTFRAFVPRLDGVGGTSTYWISPAWLDDFLGSWV
                     YLGTSQRAASTVAFVPPALGRCLGPSGSTCPFRQYAGRWATVSAHFDGPVAQTCRYHG
                     QHLGKGMTKRAAIEDCRKRLIVLSVGPDALPATDAAGEASRGHAPAGLPPITFGLALL
                     IGLLAVPRRRARRFTR"
BASE COUNT          298 a          692 c          708 g          279 t
ORIGIN      
        1 tcgtgatcgg catcgtcgcg aaggcggtct gggacacgcc gaaggcgcgg ctcgttgacg
       61 ggtaccggca acacatccgc ccgaaccacc tggacgtggg gccgggcacc ggctggttca
      121 tcgggcactc gggtctcgcg gaaggcagca gggtcacgat cctggatccg aacccgcacg
      181 tcctcgcgca cgccagccgg gagctgcggc ggttcgaggt caccgcggtc caggccgacg
      241 tcctcaagcc cctgcccgtc gaggggccgt tcgactccgc cgggttccac atggtcatcc
      301 actgcctgcc ggggccgatg acccgcaagg cccgcgcgat ggagaacatt gccgccgtgc
      361 tggcgcccaa cggcgtgctg ttcggtgcga cgatcctggg caggagcggg aatcactcgt
      421 ggctggcccg ccgggtgctg acggcgttca accgccaggg cggcatcgac aacctcgacg
      481 acagcgaagc ggggctgcgc gagatcctcg aggcgtcgtt cgagcacgtt gagatcgagg
      541 tcgtcggatc ggccgcgatc ttcgcggcga cgagcccgcg caccgcgtag tggagcgtcg
      601 ggcgtggctt gacgaacggc gactcgtgac ccggaccgtc tacgacgccg atgcgcccac
      661 ctacgacctt gacgagtacc agggcacgct gcatggggag tttgtcgccc gccttctcga
      721 tacgtgcccg cctggcggcg tcgtcctcga tgcgccgtgc ggcaccgggc actacttcgc
      781 ccaggtcacc gagtcggggc gccgggtcgt cggcatcgac cagtcggccg ggatgctggc
      841 gcaagcccgc gccaggggct tcgcggaagc cctggatcag gtcggtctcc aggacctcgt
      901 atttgccggg tccttcgacg cggtcatgtg catcgacgcg atggagaacg tcccacccga
      961 ggactggccg cttgtcctcg cgaacctgca ccgggccgtg cggcccggcg ggcatgtcta
     1021 cctcacgatc gaggagcagg cgcaggcgct cctggactcg gcgttcgagg cgcaggcggc
     1081 ccgcgggtta cccgtggtgc ggggcgagct gatcgaggga gaggcgttcg ccggctacca
     1141 ctactacccg gaccgggggc aggtcctggc ctggattgac ggggcaggat tccagctgat
     1201 tgaggaagcg tccgaggccg gacccgacgg cacctggggc taccgccact tcctgctgcg
     1261 ggacgtcgcc gtcaccgggt gaaccggcgg gcgcgtcgtc gagggacggc cagcaggcca
     1321 atgagcagtg cgagaccgaa tgtgatcggc ggcaggccgg cgggtgcgtg tccccggctc
     1381 gcttcgcccg ccgcgtccgt ggcgggaagg gcgtcggggc cgacggacag gacgatgagc
     1441 cgcttccggc agtcctcaat cgcggcccgc ttcgtcatgc ccttgccgag atgctgcccg
     1501 tggtatcgac acgtctgcgc gaccgggccg tcgaagtgcg cgctgaccgt cgcccagcgg
     1561 ccggcgtact ggcggaacgg gcatgtcgat ccgctcgggc cgaggcatcg tcccaacgcc
     1621 ggcgggacga acgcgaccgt cgatgccgcg cgttggctgg tgcccaggta gacccagctc
     1681 cccaggaagt cgtccagcca cgctggcgag atccagtagg tcgaggtccc gcccacgcca
     1741 tccaaccgcg ggacgaaggc gcggaacgtg agcagctggc cgccgtagca cgcgagcccc
     1801 gtcgcaccat cgatgcccgc gagcttggcg gcggtgaccg gggccgacgg gcagttcgca
     1861 gcccgcgcga tcgacgcgcc gccgcccgct cccggcacca ccagcaatgc ggcggcgacc
     1921 accatcacga ccgtcagcct ggcgacgaga tcgaacccgc gacccatccc aaccctc
//
LOCUS       DBNHXO010000438         2624 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5294293, whole genome shotgun sequence.
ACCESSION   DBNHXO010000438 DBNHXO010000000
VERSION     DBNHXO010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5294293"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="AC162A_08845"
     CDS             <1..493
                     /locus_tag="AC162A_08845"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5010988.1"
                     /translation="ERGPEILAGRGYSPATVRLARRFFAGRIEPDEMLPALMRFGGAY
                     YHHAGPLVLARELFAERRAKLRPEALIFAGRHLLPGWTVMDRLAEIAAPTLVIAGRDD
                     FLFPPEHQVELAAGIPGARLTIIERAGHNAHAERPEAVMAAIRAFLPAVAVAPAQAAA
                     AAG"
     gene            498..971
                     /locus_tag="AC162A_08850"
     CDS             498..971
                     /locus_tag="AC162A_08850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HIR5010989.1"
                     /translation="METIGLRHGAARDRIPASHRDLVRCPPVAALTTIGRDGYPQTSV
                     VWCDFDGECIRVNTMRSFAKERNMRRDPRVTLLCYDPREPLRYLEVRGTVIEMTEEGA
                     GAHLDELASAYAGRPIRYFGDAIPARYAASEVPVLCRIRPDHVVAMDATGSEGGR"
     gene            968..1411
                     /locus_tag="AC162A_08855"
     CDS             968..1411
                     /locus_tag="AC162A_08855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HIR5010990.1"
                     /translation="MSVAVAPDSLPIPASHLDLLTRPICGVLTTMAPDGRPESSLVWV
                     DIDGGYAAVNTTLERRKGRNIVADPRVSLLVVDPDDTGRFIQVRGDAALFTYGALDHL
                     DRLTRRYTSHDHYYGGIYPLERREHETRVIVRIHARRITLDAIHA"
     gene            complement(1420..2313)
                     /locus_tag="AC162A_08860"
     CDS             complement(1420..2313)
                     /locus_tag="AC162A_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5010991.1"
                     /translation="MSKPTRGAAALAAVLALGAIAAPVANAASVVTIRPSDLIAALSD
                     TRSAGHVEFLGDGIHAWTDDATSNAKAAEYFTLSGPIPSTASMEWFGTQPQAGQQLVF
                     DVDGVDGNGNDWNILVGEPVYGANWWLTNGSSADAKAADPSGGNDGGNGSAWFGTLAE
                     WGAALPNARVYAGGFSLGSGVKGDGVIHSITYGGTTYGFTSNPSATTTIRDVHGTSKV
                     VVRPRGVRVHLRSEAQPANTVIGKNLKWRVKVDGRVVFRASQGFGAHDVVREHFAVGS
                     GKHKIKVFKNGQRVRKVVVRF"
BASE COUNT          376 a          949 c          921 g          378 t
ORIGIN      
        1 cgagcgcgga ccggagatcc tcgccggacg cggctacagc ccggcgaccg tccgactggc
       61 ccgccgcttc ttcgccggtc ggatcgagcc cgacgagatg ctgccggcgc tcatgcggtt
      121 cggcggcgcc tactaccacc acgccggccc gctcgtgctc gctcgcgagc tgttcgccga
      181 gcgtcgcgcg aagctccggc ccgaggcgct gatcttcgcg ggccgccacc tgctgccggg
      241 ctggacggtc atggaccgcc tggccgagat cgcggcgccc acgctcgtga tcgcgggccg
      301 cgacgacttc ctgttcccgc ccgaacacca ggtcgagctc gccgcgggga tccccggcgc
      361 ccgcctcacg atcatcgagc gcgccggcca caacgcccac gccgagcggc ccgaggccgt
      421 gatggcggcg atccgcgcgt tcctcccggc cgtggccgtc gcaccagccc aggccgcggc
      481 cgccgcgggc tgaggtcatg gaaaccatcg ggctccgcca cggcgccgcg cgcgaccgga
      541 tccccgcgtc gcatcgcgac ctcgttcgat gcccgccggt cgcggcgttg acgaccatcg
      601 ggcgcgacgg ctacccgcag acctccgtcg tctggtgcga cttcgacggc gagtgcatcc
      661 gggtcaacac gatgcgcagc tttgcgaagg agcggaacat gcgccgtgac ccgcgggtca
      721 cgttgctgtg ctacgacccg cgcgagcccc ttcgctacct cgaggtccgg ggcacggtca
      781 tcgagatgac ggaggagggc gcgggcgccc acctcgatga gctcgcctcg gcgtatgccg
      841 gccggccgat ccgctacttc ggcgacgcga tccctgctcg ctacgccgca tccgaggtcc
      901 cggtcctgtg ccggatccgg ccggaccacg tcgtcgcgat ggacgcgacc ggctcggagg
      961 gcgggcggtg agcgtcgcgg ttgcgcccga cagcctgccg atcccggcct cgcacctcga
     1021 tctcctcacg cgcccgatct gcggggtgct gacaacgatg gcccctgacg gccggcccga
     1081 gtcgagcctc gtgtgggtcg acatcgacgg cggatatgcc gcggtgaaca cgaccctcga
     1141 gcgccgcaag ggtcgcaaca tcgtggccga cccgagggtc agcctcctcg tggtcgaccc
     1201 ggacgacacg ggccgcttca tccaggtccg cggcgacgcg gcgctgttca cctatggcgc
     1261 gctcgaccac ctcgaccggc tgacgcgtcg ctacacgtcc cacgaccact actacggcgg
     1321 gatctatccg ctagagcgcc gcgagcacga gacgcgcgtg atcgtccgca tccacgcccg
     1381 ccggatcacg ctcgacgcga tccacgcctg agccgtgggt cagaagcgga cgacgacctt
     1441 gcgcacgcgc tgaccgttct tgaagacctt gatcttgtgc ttgcccgatc cgacggcgaa
     1501 gtgctcgcgg accacgtcgt gggcgccgaa gccctggctg gcccggaaca cgacgcggcc
     1561 gtcgaccttg acacgccact tgaggttctt gccgatgacc gtattggcgg gctgggcctc
     1621 gctgcgcagg tggacccgca cgccgcgcgg gcgaacgacc accttcgagg tgccgtgcac
     1681 gtcccggatc gtcgtggtcg cgctcgggtt cgaggtgaac ccgtaggtcg tgccgccgta
     1741 ggtgatcgag tggatcacgc cgtcgccctt gacgcccgag ccgagcgaga agccgccggc
     1801 atagacgcgg gcgttcggca gggcggcgcc ccactcggcc agcgtcccga accacgccga
     1861 gccgttgccg ccgtcgttgc cgccggacgg atcggcggcc ttcgcatcgg ccgaggaccc
     1921 gttggtgagc caccaattgg ccccgtagac cggctcgccc acgaggatgt tccagtcgtt
     1981 gccgttgccg tcgaccccgt cgacgtcgaa cacgagctgc tgaccggcct ggggctgcgt
     2041 gccgaaccac tccatcgacg cggtcgacgg gatcggaccc gacagggtga agtactccgc
     2101 cgccttggcg ttgctggtgg cgtcgtcggt ccacgcgtgg atgccgtcgc ccaggaactc
     2161 gacgtggcct gccgaacggg tgtccgacag ggccgcgatg aggtccgacg ggcggatcgt
     2221 gacgaccgac gcggcgttcg cgacgggcgc ggcgatggcg ccaagggcca gaacggctgc
     2281 gagcgcggcg gcgccgcgcg tgggcttgga caagggaaca gacctcgatg cgtgacggat
     2341 tcgatggccc gagtgtccag ccgacgatcg ttcccctcaa tcccccgata gtgggggccg
     2401 tggagccggt cgaggggtcg tgaacctctg agctccgacc aggcgcacgc ggattgcggg
     2461 atgtcccgcc tatgcgagtg ctcgaccgta tggcgagccc gcagcgcggg ctggaggcgg
     2521 tcccgaatcg tggcgcgcgg agacgccgag tcggccactc tagggatgcg gcgaggacgc
     2581 ctccgccgac aagaggagaa cctggggtgt ccagatgaag cggg
//
LOCUS       DBNHXO010000439         1927 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2912403, whole genome shotgun sequence.
ACCESSION   DBNHXO010000439 DBNHXO010000000
VERSION     DBNHXO010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2912403"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="AC162A_08865"
     CDS             <1..353
                     /locus_tag="AC162A_08865"
                     /inference="COORDINATES: protein motif:HMM:NF037144.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5010992.1"
                     /translation="GVITYTPDPDWSGTDTFSYQVCDDQGACDTADVTVTVDPVNDQQ
                     PPQPTPTPAPHPTPTGLPASDASGAWTILTGGTAGPGGPGMAGGLLAVLALVVGGLAW
                     VALRRRGTGSDRRG"
     gene            468..>1927
                     /locus_tag="AC162A_08870"
     CDS             468..>1927
                     /locus_tag="AC162A_08870"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HIR5010993.1"
                     /translation="MSAEPQAHGGPEIENLLDERRTFPPDPAFVAQANATAALYESAN
                     ADYVGFWERQGRERLSWSKPFRETLEWDLPFAEWFVGGELNVAYNCVDRHVERGLGDR
                     VAYHWIGEPGETRTITYDDLLRETSKAANALLELGVRKGDRVAIYMPMIPELPIAMLA
                     CARIGAPHTVVFGGFSAEALGGRINDCGAKVLITADGGWRRGKQVPLKPQADAAVAAS
                     PTIEHVLVVDRLGEGASMEPGRDVWWHEIVDRQSAVCPPVAVDSEHMLYLLYTSGTTA
                     KPKGILHTSAGYLLGAAFTHWAVFDIKPDDVYWCAADIGWVTGHSYIVYGPLANATTG
                     VLYEGAPDTPAWDRWWQIVEDYKVTILYCAPTAIRAFMKQGETFPDAHDLTSLRVLGS
                     VGEPINPEAWRWYREVIGGDRTPVVDTWWQTETGQIMISPLPGVTTTKPGSATFARPG
                     IEADVVDHDGNSVPFGGGGYLVLKRPWPGMLRGIYG"
BASE COUNT          292 a          710 c          666 g          259 t
ORIGIN      
        1 ccggcgtgat cacctacacc cccgatcccg actggtcggg caccgacacc ttcagctacc
       61 aggtgtgcga cgaccagggc gcctgcgaca ccgccgacgt caccgtcacc gtggacccgg
      121 tcaacgacca gcagccaccg cagcccacgc caacgcccgc gccgcatccc acgccgaccg
      181 gcctgccggc ctcggatgcg tccggcgcgt ggacgatcct gaccggtggc accgccggcc
      241 ccggcgggcc aggtatggcg ggcggcctgc tcgcggtgct cgccctcgtg gtgggcggcc
      301 tggcctgggt cgcgctgcgt cgacgaggaa ccggttccga ccgccgcggg tagcctgcaa
      361 cgcaccgcgt gggaccacca tcccgcgagc ggcgctcgct ggccgcgtag aatgcggctg
      421 ccgggtcgag cgtcgtcacg ttcgaagccg gcgcgcagag gagtcgcatg agcgccgagc
      481 cccaggccca cggcgggccg gagatcgaga acctgctcga cgagcggcgg acgttcccgc
      541 ctgatcccgc cttcgtcgcg caggcaaacg cgaccgcggc gctgtacgag tccgcgaacg
      601 cggactacgt cgggttctgg gagcgccagg gccgcgagcg gctctcgtgg tcgaagccct
      661 tccgcgagac gctcgagtgg gacctcccgt tcgcggagtg gttcgtcggc ggcgagctca
      721 acgtggccta caactgcgtc gaccggcacg tggagcgcgg cctgggcgac cgcgtcgcct
      781 atcactggat cggcgaaccc ggcgagaccc gcacgatcac ctacgacgat ctgctgcgcg
      841 aaacctccaa ggcggcgaac gcactgctcg agcttggcgt ccgcaagggc gaccgcgtcg
      901 cgatctacat gccgatgatc ccggagctgc cgatcgcgat gctggcctgc gcccggatcg
      961 gcgcgcccca taccgtcgtg ttcggcgggt tctcggccga ggccctcggc ggccgcatca
     1021 acgactgcgg cgccaaggtg ctgatcaccg ccgacggcgg ctggcggcgc ggcaagcagg
     1081 tccccctcaa gccccaggcc gacgccgccg tggccgcatc cccgaccatc gagcacgtgc
     1141 tggtcgtcga ccgcctaggc gaaggcgcgt ccatggagcc cgggcgcgac gtctggtggc
     1201 acgagatcgt cgaccgccag tcggccgtct gcccgccggt cgccgtcgac agcgagcaca
     1261 tgctctacct gctgtacacc tcgggcacca cggccaagcc caagggcatc ctccatacct
     1321 ccgccgggta cctcctcggc gccgcgttca cgcactgggc cgtgttcgac atcaagcccg
     1381 acgacgtcta ctggtgcgcc gccgacatcg gctgggtcac cgggcacagc tacatcgtct
     1441 acgggccgct ggccaacgcg acgacgggcg tcctgtacga gggcgcgccg gacacgccgg
     1501 cctgggaccg ctggtggcag atcgtcgagg actacaaggt cacgatcctg tactgcgccc
     1561 cgaccgccat ccgcgcgttc atgaagcagg gagagacgtt tccggacgca cacgacctga
     1621 cctcgctgcg cgtgctcggg tcggtgggcg agccgatcaa ccccgaggcc tggcgctggt
     1681 atcgcgaggt catcggcggc gaccggacgc cggtggtgga cacgtggtgg cagaccgaga
     1741 ccggccagat catgatcagc ccgctgcccg gcgtgacgac gaccaagccc ggctcggcga
     1801 ccttcgcgcg gccggggatc gaagcggacg tcgtggacca cgacgggaac tcggtcccat
     1861 tcggtggagg cggctacctc gtgctcaagc gcccgtggcc ggggatgctg cgcggcatct
     1921 acggcga
//
LOCUS       DBNHXO010000440         3237 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2912506, whole genome shotgun sequence.
ACCESSION   DBNHXO010000440 DBNHXO010000000
VERSION     DBNHXO010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2912506"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="AC162A_08875"
     CDS             <1..1258
                     /locus_tag="AC162A_08875"
                     /inference="COORDINATES: protein motif:HMM:NF017050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HIR5010994.1"
                     /translation="DELFPHYRTVAETMNVQPVPRNQWPARTALMAEAAAKTGHGDRF
                     RQLELAVTFDPTWSRADPDAKDPARSRRFTNAQGRVQGTCIHLGECDIGCPVLARNTL
                     DLNYLALAEAHGADVRPLTLVRSIEPADDGYRVHVDRLDGGVRRPSTETARVVVLAAG
                     SLGSTELLLRSRDVTRTLPRLPRSLGKGWSANGDFLTIGWHPTREVRPTDGPTITSAI
                     DFRDGSSGGRPFLIEDGGFPDLLAAYTRGKTRSLSPWRRRRLLRAALRDGFGDEAAVD
                     HVMPWFSQGRDAGDGRLHLRKRWWLFGEPVLDLDWDPKASRPTIEAIIAMHRELAVAT
                     DGEPHVPPTWTAASYLITPHPLGGCAMGASPADSVVDHRGEVWGHRNLFVADGAIVPE
                     AIGANPSRTIAALAERIAGQIIAEGR"
     gene            1258..2751
                     /locus_tag="AC162A_08880"
     CDS             1258..2751
                     /locus_tag="AC162A_08880"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR5010995.1"
                     /translation="MATNDTRTRRSLILAGGGMKVGYQAGALQVLMDEAGLSFDHADG
                     TSGGCLNLAMVQSGLSGTRIANNWRATGPFELTSFQAPWRYLAPWRLPSMLTYDRLNR
                     QLPVWGVDFDAVRTSPIEGTYNVYNFTAKSFETHEGPELTPELFNACFALPVWFPPVE
                     IDGQTYIDGVYWKDANLTEAVRRGADEIWVVWTVAETAPYPHNAYGQYFAIIEAIAVG
                     RFYEELAAIEKINAAVRAGTDTAHREIKVHVVRHATPVPLDYLLFFSAGDMSRIVDMG
                     VRDTRAYLEANGVPFEPKGPLAPPIGMRFIETMRGWWTRGATEPLAGRAAGRRAGRTL
                     SVKLVITIDDMDAFLVDAEREAHATGFVDCPELGGRLAIDRGIFNVLPPAGPDGERVM
                     HYRLWFRDRMGTRYLLDGRKHVKHDAPLDVWADTTTLFVTIHAGDSTDGAVVGAGVIE
                     LRLLDLVRQATTFRVLYGADVVARLLSVLGFSRYFIGSLWNTYVLRR"
     gene            complement(2755..>3237)
                     /locus_tag="AC162A_08885"
     CDS             complement(2755..>3237)
                     /locus_tag="AC162A_08885"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5010996.1"
                     /translation="PAVAFVDLTGFTRLSEELGDEAAAALALRLGDVTVETVRGHGGR
                     VVKLLGDGVLIRFGDVATAVEATLELLDALPAAGLPTGHAGVASGPLIVRDGDIFGRT
                     VNLAARISDAAPDGHLWVTEAVAVALPADRFVVEPADGGVLQGIGRVELRDVVRSSAR
                     "
BASE COUNT          465 a         1180 c         1140 g          452 t
ORIGIN      
        1 cgacgagctg ttcccgcact accgcacggt cgcggagacg atgaacgtgc agcccgtgcc
       61 gcggaaccag tggcccgcgc gaacggcact gatggccgag gcggcggcga agaccggcca
      121 cggcgaccgg ttccggcagc tcgagctcgc cgtgaccttc gacccgacct ggtcgcgggc
      181 cgatccggac gcgaaggatc cggctcggtc ccggcgcttc acgaacgcac agggacgggt
      241 ccagggcacc tgcatccatc tcggcgagtg cgacatcggc tgcccggtcc tcgcccgcaa
      301 cacgctcgac ctcaactacc tcgcgctggc cgaggcgcac ggcgcggacg tccggccgct
      361 gaccctcgtt cgctcgatcg agcccgccga cgacggctac cgcgtccacg tcgatcgcct
      421 cgacggcggc gttcgtcgcc cgtcgacgga gaccgcgcgg gtcgtcgtcc tggcggcggg
      481 atccctgggc tcgacggagc tgctcctccg ctcgcgcgac gtgacgagga cgctgccgcg
      541 gctgccgagg tcgctgggca aggggtggag cgccaacggc gacttcctca cgatcggatg
      601 gcacccgact cgcgaggtcc ggccgaccga cggcccgacg atcacgagtg cgatcgactt
      661 ccgcgatggg tcgtcgggcg gtcgcccgtt cctgatcgag gacggcggct ttccggacct
      721 gctggccgcg tacacccgcg ggaagacccg ctcgttgagc ccgtggcggc gtcggcggct
      781 cctgcgggcg gcgctccgtg acggcttcgg cgacgaggcg gcggtcgatc acgtcatgcc
      841 ctggttctcg cagggccgcg atgccggcga tgggcgcctg cacctgcgca agcgttggtg
      901 gctgttcggc gagccggtgc tcgacctcga ctgggacccc aaggcaagcc ggccgacgat
      961 cgaggcgatc atcgccatgc atcgcgagct cgcggtcgcg accgatggcg agccgcacgt
     1021 gccgcccacc tggacggcgg cgagctacct gatcacgccc cacccgctcg gcgggtgtgc
     1081 gatgggggcg agccccgccg acagcgtcgt ggaccaccgg ggcgaggtct ggggtcatcg
     1141 caacctgttc gtcgccgatg gcgccatcgt ccccgaggcc atcggcgcca acccgtcccg
     1201 gacgatcgcc gcgctcgcgg agcggatcgc gggtcagatc atcgcggagg gccgctgatg
     1261 gccacgaacg acacgagaac ccgtcgctcg ctgatcctcg caggcggggg catgaaggtc
     1321 ggctaccagg ccggcgcgct ccaggtgctg atggacgagg cgggtctctc gttcgaccac
     1381 gccgacggca ccagcggcgg ctgcctcaac ctcgcgatgg tccagtcggg cctgtccggc
     1441 acccggatcg ccaacaactg gcgcgcgaca ggaccgttcg agctgacctc gttccaggcg
     1501 ccgtggcggt acctcgcccc gtggcgcctg ccgtcgatgc tcacctacga ccgcctcaac
     1561 cggcagctcc ccgtgtgggg cgtggacttc gacgccgtcc gcaccagccc gatcgagggc
     1621 acctacaacg tctacaactt caccgcgaag tcgttcgaga cccacgaggg ccccgagctc
     1681 acgcccgagc tgttcaacgc gtgcttcgcg ctgcccgtct ggttcccgcc ggtcgagatc
     1741 gacggtcaga cctacatcga cggcgtctac tggaaggacg ccaacctgac cgaggccgtc
     1801 cgccggggcg ccgacgagat ctgggtggtt tggaccgtcg ccgagaccgc gccctacccc
     1861 cacaacgcgt acggccagta cttcgcgatc atcgaggcga tcgcggtcgg gcggttctac
     1921 gaggagctgg cggcgatcga gaagatcaac gccgcggtgc gggccggaac tgacaccgcg
     1981 catcgcgaga tcaaggtcca cgtcgtccgc cacgcgacgc ccgtcccgct cgactacctg
     2041 ctgttcttct cggcgggtga catgagccgg atcgtggaca tgggcgtccg cgacacgcgc
     2101 gcgtacctcg aggcgaacgg cgttccgttc gaaccgaagg ggccgctcgc gccgccgatc
     2161 gggatgcggt tcatcgagac gatgagaggc tggtggacga gaggggcgac ggaacccctg
     2221 gccggccgcg ccgccggcag gcgcgccggc cggacgctgt cggtgaagct ggtgatcacg
     2281 atcgacgaca tggacgcgtt cctcgtcgat gccgagcgcg aggcccacgc gacggggttc
     2341 gtcgactgcc cggagctggg cggtcgcctg gccatcgacc gcggcatctt caacgtcctg
     2401 ccgccggccg gaccggacgg cgagcgcgtc atgcactacc ggctgtggtt ccgcgaccgg
     2461 atgggcaccc gctaccttct cgacggccgc aagcacgtga agcacgacgc gccgctcgac
     2521 gtctgggccg acacgacgac cctgttcgtg accattcacg ccggtgactc caccgacggc
     2581 gcggtcgtgg gagccggtgt catcgagctc cgcctgctcg acctcgtgcg ccaggcgacg
     2641 acgttccgcg tcctctacgg ggcggacgtg gtggcccggc tgctctcggt cctggggttc
     2701 tcgcgctact tcatcggctc gctctggaac acgtacgtct tgcgacgctg agcgtcagcg
     2761 ggccgaactc ctcacgacgt cgcgcagctc gacccgcccg atcccctgga ggacgccgcc
     2821 gtcggcgggc tcgaccacga agcgatcggc gggcagggcg acggcgaccg cctcggtcac
     2881 ccagaggtgg ccgtcgggcg ccgcgtcgga gatccgggcc gccaggttca cggttcgccc
     2941 gaagatgtcg ccgtcgcgca cgatgagggg gccgctcgcc acgccggcgt ggccggtggg
     3001 gaggccggcc gccgggagcg cgtcgaggag ctcgagggtc gcctccacgg cggtcgccac
     3061 gtcgccgaac cggatcagga cgccgtcgcc caggagcttg acgacccggc cgccgtgccc
     3121 gcggaccgtc tccaccgtca cgtctcccag ccggagcgcc agcgccgccg ccgcctcgtc
     3181 gccgagctcc tcggacaggc gcgtgaaccc cgtgaggtcg acgaaggcga cggccgg
//
LOCUS       DBNHXO010000441         2607 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1721749, whole genome shotgun sequence.
ACCESSION   DBNHXO010000441 DBNHXO010000000
VERSION     DBNHXO010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1721749"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2122)
                     /locus_tag="AC162A_08890"
     CDS             complement(<1..2122)
                     /locus_tag="AC162A_08890"
                     /inference="COORDINATES: protein motif:HMM:NF036968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR5010997.1"
                     /translation="MSVRWIREGPPSRELERRRDEDRAMDREVARRLDPETGILMAAE
                     RRFDASRMDLPIGGPSALDRPIDRHAIDDGGGPGDESAAARRWARDQVAMRADRSNSI
                     MKMPPQAELRPPDEHPHRGSVANLSDWERWLELCARIDGFPRHLSIHSGGMLVTAAPL
                     IDIAPIERATMKDRVVVQFDKRDVETMKLIKLDLLGLGMLAAIDECVQLIEHDCAVCL
                     DLDHIPEEIPEVFEMLQAADTVGVFQVESRAQMQTLPKSRPQSLDDLVVEVAIIRPGP
                     IQGNAVHPYLRRKQGIEPVVYLHQSLAPILRDTLGVILYQEQVMKIAIEVAGFTPAGS
                     DGFRRAMGTWRSTREMEKLHEQFVEGCISVQGMPRDDAEELFRQCAAFASFGFAKSHA
                     AAFARTAYESSFLKLFYPAQFVTGLINAQPMGFYPVEVLVNDAKRHGVAVLPVDVNRS
                     SWRTTTEWVGRPGWALAGADGDDGSHDGDPGEPLPDGCGIDALPAPVRSPACVIPGAA
                     SRDRWAAESMTGWGVRLGLHLVKGIGEEQEARLDAELARGPYTSLADVVERTGLAEEV
                     VERLIRAGALDSLGRPRRELLWQLREVAGASKGRVGGKARGTKGITRAAGRPMDLRLP
                     ATEAPELPPLTETERLGDAYAVVGLDARLQAVALFRGALDRLGAVPNGILNERRPGPV
                     RLGGLVVTRQHPMTARGTVFLALED"
BASE COUNT          337 a         1009 c          895 g          366 t
ORIGIN      
        1 cgtcctccag cgcgaggaac accgtcccgc gcgccgtcat cgggtgctgg cgcgtcacca
       61 ccagcccacc gaggcgcacg ggtcccggcc gtcgctcgtt caggatcccg ttcggcaccg
      121 cgcccagccg gtcgagcgcg cctcggaaca gcgcgacggc ctggagtcgg gcgtccagcc
      181 ccacgaccgc gtaggcatcc ccgaggcgct cagtctcggt gagcggcggg agctccggcg
      241 cctcggtggc gggcaggcgg aggtccatcg gccgcccggc ggcccgggtg atgcccttcg
      301 tgccgcgcgc cttcccgccg acccggccct tcgacgcccc cgcgacctcg cgcagctgcc
      361 acagcagctc ccgccgtggc cgtcccagcg agtccagggc ccccgcgcgg atcagccgct
      421 ccacgacctc ctccgcgagc cccgtccgct ccaccacatc ggccagtgac gtgtacggcc
      481 cgcgcgccag ctcggcgtcc aggcgcgcct cctgctcctc gccgatcccc ttcaccaggt
      541 gcagcccaag ccggacgccc cagcccgtca tgctctcggc cgcccatcga tcgcgcgagg
      601 ccgccccagg gattacgcac gcgggcgagc gcacgggcgc cggcagggcg tcgatcccgc
      661 agccgtcggg cagcggctcg cccggatcgc cgtcgtggct cccgtcgtcg ccatcggccc
      721 ccgccagcgc ccagcccggc cgtcccaccc actcggtcgt cgtccgccac gagctccggt
      781 tgacgtcgac cggcagcacc gcgacgccgt ggcgcttcgc gtcgttgacc agcacctcga
      841 ctgggtagaa gcccatcggc tgggcgttga tcagccccgt gacgaactgg gcggggtaga
      901 acagcttgag gaagctcgat tcgtaggccg tccgggcgaa ggcggcggcg tggctcttcg
      961 cgaacccgaa cgaggcgaac gcggcgcact ggcggaacag ctcctcggca tcgtcgcgcg
     1021 gcatgccctg gacgctgatg caaccctcga cgaactgctc gtggagcttc tccatctcgc
     1081 gcgtcgagcg ccacgtgccc atcgcccgcc ggaacccgtc cgagcccgcc ggggtgaagc
     1141 ccgcgacctc gatcgcgatc ttcatcacct gctcctggta caggatcacg cccagggtgt
     1201 cgcgcaggat cggcgccagg ctctgatgga ggtagacgac cggctcgatg ccctgcttgc
     1261 ggcgcaggta ggggtggacg gcgttgccct ggatcgggcc gggccggatg atcgccacct
     1321 ccacgaccag gtcgtcgagg ctctgcggcc gcgacttggg cagcgtctgc atctgggccc
     1381 ggctctcgac ctggaacaca cccacggtgt cggcggcctg gagcatctcg aacacctccg
     1441 ggatctcctc cgggatgtgg tcgaggtcga ggcacacggc gcagtcgtgc tcgatgagct
     1501 ggacgcactc gtcgatggcg gccagcatgc cgaggccgag caggtcgagc ttgatcagct
     1561 tcatggtctc gacgtcgcgc ttgtcgaact ggacgacgac gcggtccttc atcgtcgcgc
     1621 gctcgatggg ggcgatgtcg atcagggggg cggccgtcac gagcatcccg cccgaatgga
     1681 tcgagaggtg gcgggggaag ccgtcgatgc gggcgcacag ctccagccag cgctcccagt
     1741 ccgagaggtt ggccaccgag ccgcggtgcg gatgctcgtc gggcggccgg agctcggcct
     1801 ggggcggcat cttcatgatc gagttgctgc ggtccgcccg catcgcgacc tggtcgcggg
     1861 cccatcgccg cgccgccgcg ctctcgtcgc ccgggccgcc cccatcgtcg atcgcgtgcc
     1921 gatcgatcgg gcggtcgagc gcgctcgggc cgccgatcgg gaggtccata cgcgaggcgt
     1981 cgaatcgccg ttccgcggcc atcaggatcc cggtctcggg atcgaggcgg cgcgcaacct
     2041 cgcggtccat tgcccggtcc tcgtcccggc gccgttccag ctcccggctg ggcggcccct
     2101 cgcggatcca gcgcacgctc accggtgaat cgccggggcc gcccttgtcg gcgacctccc
     2161 ggataccgcc ccccgtggcg gtatccgcac ccggcgccag caccccgacc ttgttccgga
     2221 gccaccggtc cgcctcgtcg cccccaagcc atcgctcccc gcgccgcgcc gcgcctccgg
     2281 tcgggtcggt cgcgatacgc ctaggtggag tactggacgc atagccgccg tccgcagggc
     2341 tcgatccggc ctcgcctacg cctccccgcg gtatccgggt cggatcggcg cgtgagccgg
     2401 cgataccact ccacccaggc gtagacgagc cggaaccggc ggaggcagcg gcttcgaagt
     2461 ggccgccgcg agggtggttc agctggccca tcgcgtcggt caggccgtgg gcggcggcca
     2521 gggacgcagc caccggtgcc gcagcctccc cgggcacctc ccccggccgg gcgaagaact
     2581 gcgcgaagcc gccctccgcc tcgaggt
//
LOCUS       DBNHXO010000442         5463 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5494551, whole genome shotgun sequence.
ACCESSION   DBNHXO010000442 DBNHXO010000000
VERSION     DBNHXO010000442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5494551"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..1143
                     /locus_tag="AC162A_08895"
     CDS             304..1143
                     /locus_tag="AC162A_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HIR5010998.1"
                     /translation="MTVVRANRLFNAVSGNCINVAIDHGITGELELLGGIVDLPHAVD
                     AVVGAHPDAVQLAVGSAHLLQERQGAQKPALVLRVDFANIYGNDIPDVRFCSIMEEAV
                     VQAVRLDAAAVVCNLFDVADAPEIRQQCVHNILRLRADCSAYQMPLMVEPLVFRREKG
                     GPYVSDGDVRRIVPLVRQAVELGADVIKADPTDDLDDYDQVIAVSTSPVLVRGGGRVT
                     DEVVLRRTEQVMRAGARGIVYGRNIVQHPQPGAMVKALSSIVHGGGSADKALAMLGAD
                     GAS"
     gene            1140..2282
                     /locus_tag="AC162A_08900"
     CDS             1140..2282
                     /locus_tag="AC162A_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5010999.1"
                     /translation="MSEIGVGLIGGGLMGKELAPTLARWPSLQQPGSTPVLRAVCDTN
                     PAVLGWFSRVASVQTTTDDYRRLLDDPSIEVLYIAVPHHLHERIFIDAIEAGKDVMAE
                     KPFGLDLDAARVILAAVRGRPDAFVRVSSEIPFFPGAQKAIQAAVDGELGEVLDVRVG
                     LEHAGDLDRTKPINWKRQRAYCGPGGVMADLGMHALHAPLRIGWLPETLFAVLTNMVE
                     ERPGPDGEPVACDTFDNATLLATLPATATAVAPMIVDMKRISPGELNTWRFRAVGMSG
                     GVEYTTRYPGTLRRFTVREGRQGWVEEGTGTASVSPTSIPAAVEFGFTDAVFQMWAAF
                     FAERDGSLGDRFGCATPDEAFATQRIFTAALRSHERREAVELAAIQ"
     gene            2312..3307
                     /locus_tag="AC162A_08905"
     CDS             2312..3307
                     /locus_tag="AC162A_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR5011000.1"
                     /translation="MHDVARVAGVSQKTVSRVATGDPGVSEATRATVESAIASLGYRR
                     NDAARQLRPGQRTMLVGLAIGDIGNPWFSELASAVEKALNRSGYLVIIGSTADDPQRE
                     VRIVSQLCERRVDGLLVVPTAGDHRYLETERNAGTSIVFLDRPAVDLESDCVLPRHRA
                     ASRDAVRHLVTHGHRRIAFVGASERLGYAATERLAGYREALRAARIAFDAELVQLGSP
                     DAEAGGTAMRALLRLPRPPTAVFAANNRASIGVLGELGPDLGGLAIVGFDDFELARRL
                     PIPLTVVANDPAEMGHRAVDRLLRRMAGDEGPARRVFVPTRLHVRGSGEIPPPVR"
     gene            3307..4644
                     /locus_tag="AC162A_08910"
     CDS             3307..4644
                     /locus_tag="AC162A_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HIR5011001.1"
                     /translation="MDTAPRFAGLDIGTSRIKAVVIDVTGRPLSTAATPTPWTSAGTE
                     IDAGSLLAAALACLGDALDRVPGAPPVALGIASIAETGFLCDPTDIELTRGIAWHDPR
                     GRGAVTRLASDVGERSFAETTGLPLSATASIAKMDWLLAQGSFPRGTTWLGVAEWLAV
                     ALGAQPRAELSLASRTGLLDIASRAPSSDLTAWLGRPEILPPLVVAGEPVGVVTARFE
                     RLAGAVLVLAGHDHLCAAVGAGAQAQTDLLNSCGTGEAFIASTGEPMSRERIGDAVER
                     GLNVGWHVLAGRRSVIGSLATGVALTRVLELIGAPHGFASLEGPRARRDPDSSRVRID
                     GLFDETLQVRVTGAGVTPDEVWAAAVAEAHDHSMLAYDRIRDWMGGFERVVGTGGWLR
                     SREVLGRKVRDLAGFSVAPAQECAALGAALLAGVGAGFWPDAESVPRLGAAVG"
     gene            complement(4570..>5463)
                     /locus_tag="AC162A_08915"
     CDS             complement(4570..>5463)
                     /locus_tag="AC162A_08915"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018877.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HIR5011002.1"
                     /translation="LLDAALDVLNRHFWDERAGAVVDSWSRGWRVLDDYRGANANMHA
                     VEALLAASAAAGSARCLEQALRILDRFVNHAARDNDWRMPEHYTAAWIADRSFNADAP
                     FDMFKPYGVTPGHLFEWAKLCCQAAAAAGPSAPSWLVPAARRLFARAAADGWLVDGAP
                     GLVYTVDWDGSPRARQRLHWVLAEAIGAAALLAQTTGEATYRDWYRTWWAFAEEHLID
                     REQGSWRHELDATLEPSSTIVAGKPDVYHSLQACLYALVPPGPSIAAALASDPGPSAD
                     GGAEPGHRLGVGPEPGADTGE"
BASE COUNT          744 a         1963 c         1979 g          777 t
ORIGIN      
        1 cgaacacgag gttctggtgc tgcgcatggc ggatcgtatc gacgcgcgag atcctcatcg
       61 cgttcgcgtc gcgctgctcg acgaaccctg ggcaacgtca cccgagccgg gtgccgggat
      121 ggggaaaatc attccgtgtt atgatatgag cacacgtttg cgcgtgcaag tgacggccac
      181 cccggcccgc gaggccatgc gagcagccgt cgatccccgg taccgatcga ccaactgtgt
      241 cagcgaccga cacgctagac tacgtagtca tccgcggatc gcacgcggaa ggaggcccag
      301 cccgtgaccg tcgttcgagc caaccgcctg ttcaacgcag tgtccggaaa ctgcatcaac
      361 gtggccatcg accatgggat caccggcgag ctcgagctcc tcggcgggat cgtcgacctg
      421 ccgcatgccg tggacgccgt ggtcggcgcc caccccgatg cggttcagct tgcggtcggg
      481 tccgcccacc tgctccagga gcgccaaggg gcgcagaagc ccgccctcgt gctgcgcgtc
      541 gacttcgcca acatctacgg caacgacatc cctgacgtcc ggttctgctc gatcatggag
      601 gaggccgtgg tgcaggccgt ccggttggat gcggccgccg tcgtgtgcaa cctgttcgac
      661 gtcgccgacg ccccggagat ccggcagcag tgcgtgcaca acatcctccg gctgcgggcc
      721 gactgctccg cgtaccagat gccgctgatg gtcgagcccc tcgtgttccg ccgcgagaag
      781 ggcggcccct acgtcagcga cggagacgtg cgccggatcg tgccgctggt ccggcaggcg
      841 gtggagctcg gcgccgacgt catcaaggcc gacccgaccg acgacctcga cgactacgac
      901 caggtcatcg ccgtctcgac gtccccggtc ctcgtccggg gcggcggacg ggtgaccgac
      961 gaggtggtgc tgcgccgaac ggagcaggtc atgcgggccg gcgcccgggg catcgtctat
     1021 ggccgcaaca tcgtccagca cccgcagccc ggcgcgatgg tcaaggcatt gagctcgatc
     1081 gtgcacggcg gtggatcggc ggacaaggcg ctcgcaatgc tcggagccga cggcgcctcg
     1141 tgagcgagat cggcgtcggg ctgatcggcg gcgggctgat gggcaaggag ctggcgccca
     1201 ccctcgcgcg ctggccctcg ctccagcagc cgggctcgac gcccgtcctg cgcgccgtgt
     1261 gcgacacgaa cccggccgtg ctcggttggt tctcgcgcgt cgcctcggtc caaacgacga
     1321 ccgacgacta ccggcggctg ctcgacgacc cctcgatcga ggtcctgtac atcgcggtcc
     1381 cgcaccacct gcacgagcgg atcttcatcg acgcgatcga ggccgggaag gacgtcatgg
     1441 cggagaagcc gttcggcctc gacctcgacg ccgcgcgagt catcctcgcg gccgtccggg
     1501 gacggcccga cgcgttcgtc agggtctcga gtgagatccc cttcttcccc ggcgcccaga
     1561 aggcgatcca ggcggcggtc gacggcgagc tgggggaggt cctcgatgtt cgcgtcggcc
     1621 tcgagcacgc cggcgacctc gatcgaacca agccgatcaa ctggaaacgg cagcgtgcct
     1681 attgcggacc tggcggggtc atggccgacc tgggcatgca tgcgctgcat gccccgctcc
     1741 ggatcggctg gctccccgag acgctgttcg ccgtcctgac caacatggtc gaggagcggc
     1801 cggggcccga cggcgagccg gtcgcgtgcg acaccttcga caacgcgacg ctgctggcga
     1861 ccttgccggc gaccgcgacc gcggtcgcgc cgatgatcgt ggacatgaag cggatctcgc
     1921 ccggtgagct gaacacctgg cgcttccggg ccgtcggcat gtccggcggg gtcgagtaca
     1981 cgacccgcta tccggggacg ctgcgacggt tcacggtccg cgagggccgc cagggatggg
     2041 tcgaggaggg gacggggacg gcatccgtca gcccgacctc gatccccgcg gcggtcgagt
     2101 tcggcttcac cgatgccgtg ttccagatgt gggcggcgtt cttcgccgag cgggacggct
     2161 ctttgggcga tcgcttcggc tgtgcgacgc cggacgaggc gttcgccacc cagcggatct
     2221 tcaccgcggc cctgcgctcg cacgagcggc gcgaggcggt cgagctcgcg gccatccagt
     2281 agccggttgt cacgtgagcc ctcgaccgac gatgcacgac gtggcccggg tggcgggggt
     2341 gagccagaag accgtgtccc gggtcgcgac cggcgatccc ggcgtgagcg aggcgacgcg
     2401 cgcgaccgtc gagtccgcga tcgccagcct cggctaccgg cgcaacgatg cggcccgcca
     2461 gctgcggccc ggccagcgca cgatgctggt gggattggcc atcggtgaca tcggcaaccc
     2521 ctggttctcc gagctcgcga gtgcggtgga gaaggcgctc aaccgcagcg gctatctcgt
     2581 gatcatcggc agcacggccg acgaccccca gcgcgaggtc cggatcgtca gccagctgtg
     2641 cgagcgccgg gtcgacggcc tgctcgtcgt cccgacggcc ggggaccatc gctacctcga
     2701 gacggagcgc aacgccggga cctcgatcgt gttcctcgac cgcccggcgg tggacctcga
     2761 gagcgactgc gtgctccccc gccaccgcgc ggccagccgg gacgcggtgc gccacctcgt
     2821 cacgcacggc caccgcagga tcgccttcgt cggcgcgtcg gaacggctgg gctatgcggc
     2881 caccgaacgg ctggccggct atcgcgaggc ccttcgcgcc gcccgcatcg cgttcgacgc
     2941 ggagctcgtg cagctcggct cgcccgacgc ggaggccggc gggacggcga tgcgcgccct
     3001 cctccgcctg ccgcgaccgc ccacggcggt gttcgccgcc aacaaccggg catcgatcgg
     3061 cgtcctcggc gagctcggcc ccgacctcgg tggcctcgcg atcgtcggct tcgatgactt
     3121 cgagctggca cggcgcctgc cgatcccgct gaccgtcgtc gccaacgatc ccgccgagat
     3181 ggggcacagg gccgtggacc ggctgctgcg ccgcatggcc ggcgacgagg gtcctgcgag
     3241 gcgcgtgttc gtgccgaccc gcctgcacgt gcgcggctcc ggtgagatcc cgccgcccgt
     3301 ccgctgatgg acaccgcacc gcggttcgcc ggcctcgata tcggcacctc gcgcatcaag
     3361 gccgtggtga tcgacgtgac cgggcgaccg ctctcgacgg ccgcaacgcc gacgccgtgg
     3421 acgagcgcgg gcaccgagat cgacgctggg tccctgctgg cggcggcgct ggcttgcctc
     3481 ggcgatgccc tcgaccgtgt gcccggagcc cctccggtgg cgctcggtat cgccagcatc
     3541 gcggagaccg ggttcctgtg cgacccgacc gacatcgagc tcacccgcgg gatcgcctgg
     3601 cacgacccgc ggggccgcgg ggcggtcacc cggctggcgt cggacgtcgg cgagcggtcg
     3661 ttcgccgaga cgaccggcct gccgctgtcg gcgaccgcca gcatcgccaa gatggactgg
     3721 ctcctcgccc agggatcgtt cccacgcggc acgacctggc tcggggtcgc cgagtggctc
     3781 gccgtggcgc tcggggcgca gccgagggcc gagctctcgc tggcctcccg gaccggcctg
     3841 ctcgacatcg cgagccgcgc gccgagcagc gacctgaccg cctggctcgg tcggcccgag
     3901 atcctgccgc cgctggtcgt ggcgggtgag ccggtcggtg tcgtaactgc gcggttcgag
     3961 cggctggccg gggcggtgct cgtcctcgcg ggccacgacc acctgtgtgc cgccgtgggc
     4021 gcgggcgcgc aggcgcagac cgacctgttg aactcgtgcg ggaccggcga ggcgttcatc
     4081 gcttcgaccg gcgagccgat gtcgcgcgag cggatcggcg acgccgtgga gcggggattg
     4141 aacgtcggct ggcacgtgct tgccggtcgc cggtcggtca tcggcagcct cgccaccggc
     4201 gtggcgctga cccgcgtgct cgagctgatc ggcgcgccac acggcttcgc gtcgctcgaa
     4261 ggccctcggg cccggcgcga ccccgactcg tccagggtcc gcatcgacgg cctgttcgac
     4321 gagacgctgc aggtccgggt cacgggcgcc ggcgtgaccc cggatgaggt gtgggccgcg
     4381 gcggtggccg aggcgcacga ccactcgatg ctcgcctacg accggatccg cgactggatg
     4441 ggcggattcg agcgcgtcgt ggggacaggc ggctggctgc gcagtcggga ggtgctgggc
     4501 cgcaaggtcc gcgacctcgc cggcttctcc gtggcgccgg cgcaagagtg cgccgcgctg
     4561 ggcgctgccc tactcgccgg tgtcggcgcc gggttctggc ccgacgccga gtcggtgccc
     4621 cggctcggcg ccgccgtcgg ctgacgggcc ggggtcggac gcgagcgcgg cggcgatcga
     4681 cgggcccggc gggaccagcg cgtagaggca ggcctgcagc gagtggtaga cgtcgggctt
     4741 gccggcgacg atcgtgctcg acggttcgag cgtcgcgtcc agctcgtggc gccatgaccc
     4801 ctgctcgcgg tcgatcaggt gctcctcggc gaacgcccac cacgtccgat accagtcccg
     4861 gtacgtcgcc tcgcccgtcg tctgtgcgag gagcgccgcc gccccgatcg cctcagccag
     4921 cacccagtgc aggcgctgac gagccctcgg cgacccgtcc cagtcgacgg tatagacgag
     4981 acccggggca ccatccacca gccagccgtc cgcggcggcc cgggcgaaca gtcgccgtgc
     5041 ggcggggacc agccacgagg gagcgctggg gccggcggcc gcggccgcct ggcagcacag
     5101 cttggcccac tcgaacagat ggcccggcgt gacgccgtag ggcttgaaca tgtcgaacgg
     5161 cgcgtcggcg ttgaacgagc gatccgcgat ccaggccgcc gtgtagtgct cgggcatgcg
     5221 ccagtcgttg tcgcgagccg cgtgattgac gaaccggtcg aggatccgga gcgcctgctc
     5281 gaggcaccgg gcggagccgg cggccgcgct cgccgcgagg agcgcctcga ccgcgtgcat
     5341 gttggcgttg gcgccgcgat agtcgtccag cacccgccag ccgcgactcc acgagtcgac
     5401 gaccgcgccc gcccgctcgt cccagaagtg gcggttcagc acgtccaggg cggcgtcgag
     5461 gag
//
LOCUS       DBNHXO010000443         7219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4700081, whole genome shotgun sequence.
ACCESSION   DBNHXO010000443 DBNHXO010000000
VERSION     DBNHXO010000443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4700081"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1196
                     /locus_tag="AC162A_08920"
     CDS             51..1196
                     /locus_tag="AC162A_08920"
                     /inference="COORDINATES: protein motif:HMM:NF018805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HIR5011003.1"
                     /translation="MKRFSPILPLLLVAVFVAGCSGPVLANPAAAHPVVTPLPPTSPR
                     PADPVPIVLPDDDAPHDRLTEWWYDTGHLRAADGRRFGFEFVVFRAERGGFPVSWASH
                     LALTDETGQAFHYAQRSEIGPQVDRSPRDGDAPLEYAFVVEGGGRTPWTMAGGDGSDE
                     LHASATGDEVGGDPVSGFGIDLRLTARKPAALHGGDGWIDFGPAGGSYYYSRTDMAAT
                     GTVTLGGETLEVEGEAWFDHQWGDFISVGGGGWDWFAVNLDDGTDLTLSLVRAADGTY
                     PLVYGTLVDADGSTTDLGADDFSVEVTDEWTSPATGATYPAAWRVRVPSQALEIDLRP
                     TVAQQELDTRATTGVVYWEGSQVVTATRDGRRLGGQAYVELTGYAKP"
     gene            complement(1193..2500)
                     /locus_tag="AC162A_08925"
     CDS             complement(1193..2500)
                     /locus_tag="AC162A_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006378379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuconate dehydratase"
                     /protein_id="HIR5011004.1"
                     /translation="MTRITGLDVIDLRFPTSRSLDGSDAMNPDPDYSAAYVILRTDAA
                     DGLEGHGFTFTIGRGTEVVVAAVEALRPLIVGLDVEGIEADLGAFWRAMVADSQLRWI
                     GPEKGAIHLATAAVVNGAWDLVAKRAGKPVWRLLADMTPAEIVALVDFRYLTDALTPD
                     EALALLEAAAPGREARTAELLAGGYPASTTSVGWLGYDDAKIRRLAREALADGWTRFK
                     MKVGADVEDDVRRATILREELGAERMLAVDANQRWDVGSAISWMERLRPFDPYWIEEP
                     TSPDDVLGHAAIARAVAPIRVATGEHVQNRIMFKQFLAAGAMDVCQIDACRLGGVNEV
                     IAVLLLAAKFGVPVCPHAGGVGLCELVQHLAAFDYIAVSGSLDGRMIEYVDHLHEHFV
                     DPVLIRQGSYLLPTTPGYSAEIKAETLAAFHYPDGAEWRRTLG"
     gene            complement(2505..3530)
                     /locus_tag="AC162A_08930"
     CDS             complement(2505..3530)
                     /locus_tag="AC162A_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5011005.1"
                     /translation="MQNPFRRRSTTDLSPPAKFVAWLMLLGWTFVVLFPIYWLFVTSF
                     KTPYQVDKGPFYIPFVDFVPTLDAWRYILVELGNDTLRPYVNTVIVGLSSSLIALLLG
                     SVTAYALVRFTYRPKVALIGLFIGCLVLAFVGMALGGPPALMLAAAFAVFLILAQTIG
                     RRFTWALANSDIAFWLISQRILPPIAVAVPLYVLFQQVGLVDNIAALTVTYVATNIPI
                     VVWLMRDYFMSLPRELEESAAVDGASIYRIVRSIVLPISVPGLVATFLIVLIFSWNEF
                     LIALVLTSADAQTMPLQVAAQNATRGPQWWAMSVLILIMIAPVVVMAIALERFITRGL
                     LVGALKG"
     gene            complement(3534..4682)
                     /locus_tag="AC162A_08935"
     CDS             complement(3534..4682)
                     /locus_tag="AC162A_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5011006.1"
                     /translation="MASSASQIEAARSRASTTLGAWRSDGAARWLFIWPTVIVILSLS
                     LFPLIASVVMSLSRLAFNRGGVELKFVGFANYQQLLFGLERSHFLGVLKPPNPLGWII
                     VGLTIVLVTIAWTRAARSGQTGPFGLVLRLFAGVLLVGFVYLLVQALFSEGGRPGSLI
                     VTLIFVFAGIALQVGLGLGLALLAVQQVPWRRFFRVVFLIPLTITPVGVGYMFLMMTD
                     TSKGPLEPLWVSLGLRDFTWVTDPWLARAAVIIGDTWQWTPFVFIVLLAALESLDQEV
                     SEAATVDGASRWQSFLHITMPALLPVTTTIVLIRLIEGFKIIDMPNILLGGGPGTATQ
                     SLTLQAWIDWNTLNLGRSAAVAYLLLIVVTIVATVYVSYVRRRVTTVV"
     gene            complement(4666..6264)
                     /locus_tag="AC162A_08940"
     CDS             complement(4666..6264)
                     /locus_tag="AC162A_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5011007.1"
                     /translation="MSRFRSIAPAVGLVLVLAGCAAQSPTTAPVASTPATAAPASVAA
                     PTEAASEAAPSEAAVTPAACATDGKFAGANVNILTFNGPQVAEPLQRRAPDWACLTGG
                     HVNVVAVGFQTIYDKALLDASTGTNAFDAYVFDPQWLGDFTGPGYLADLTDRVNADAQ
                     LDWQDIGPFFRDFNATYNGKVYTIPLDGDFHMVYYRSDILEKDGVAPPKTWDDYLAVA
                     QKYQGQDLNGDGEPDYGSCIAKKKGAQSYWWIISIAAGLLQGKGTGDGAFFDTTNMNP
                     LFGPNEAMTKALQTYKKTAEFGPPDELNMDVGGTRGLFTTGRCALTMDWGDIGTLTPG
                     TYAQDKTGATITPGWTQVLDRASGKLVACDATTCPDAVDGVNYAPFASFGGWSGAINA
                     ASPAANQDAVYDFLSYMASPAISGEDVTLGKTGYNPYRTSHFSNLAPWVAAGLSEAAA
                     TNYLGAIKSSLENKNMVLDMRIPQTKRYQQDVLDTAVAQYLAGELDEAGTGQAIADGW
                     NAITDEVGKNDQLAAYVASLGVQR"
     gene            6438..>7219
                     /locus_tag="AC162A_08945"
     CDS             6438..>7219
                     /locus_tag="AC162A_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005473578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR5011008.1"
                     /translation="MSNETLERDAEAESLAPAVTRAALILGVMAETASEPVGPSELAR
                     RLGLPKSSIANICGALADAGLVRRIGTGFALGRRLAELGGAYLGSVDQVQEFYDVARQ
                     LATASEETVQFAVLDGLEVTYLARHDGRQPVRLTSGIGRRLPAFSTATGKAALASLPD
                     DELDRRLAGLTTLPQPSPKSHATVDSLRRDLATIRERGYAMDDEETMEGVVCFGTMIP
                     SRQPGEGPCAASITLLKVRATSERVPALLDDLRLLAAELSDPL"
BASE COUNT         1145 a         2500 c         2557 g         1017 t
ORIGIN      
        1 atcggccggg tgctgccgcg ctccgaggcc gaggccgagg cggccgcgcg atgaagcgct
       61 tctcaccgat cctcccactc ctcctcgtcg ccgtcttcgt cgccggttgc agcgggccgg
      121 tgctcgccaa ccccgcggcc gcgcacccgg tcgtgacccc actaccgccg acgtcgcccc
      181 gcccggcaga ccccgtcccg atcgtgctcc ccgatgacga cgcgccgcac gaccgcctca
      241 ccgagtggtg gtacgacacc ggccacctgc gcgcggccga cggccgccgg ttcgggttcg
      301 agttcgtcgt cttccgcgcc gagcgcggcg gcttcccggt gtcatgggcc tcgcacctcg
      361 cgctgaccga cgagaccggc caggccttcc actacgcgca gcggagcgag atcgggccgc
      421 aggtcgaccg ctcgccacgg gacggtgatg cgccgctcga gtacgcgttc gtcgtcgaag
      481 ggggcggccg gacaccgtgg acaatggccg gcggcgacgg cagcgacgag ctgcacgcct
      541 cggcgacggg cgacgaggtc ggcggggacc cggtgagcgg gttcgggatc gacctccggc
      601 tcacggcccg caagccggcg gcgctccacg gcggcgacgg ctggatcgac ttcgggccgg
      661 ccggcggctc gtactactac tcgcggaccg acatggccgc gaccgggacg gtcacgctgg
      721 gcggcgagac gctcgaggtc gagggcgagg cctggttcga ccaccagtgg ggcgacttca
      781 tctcggtcgg cggcggcggg tgggactggt tcgccgtcaa cctcgacgac gggacggacc
      841 tcacgctgtc gctggtccgg gccgccgacg gcacctaccc gctggtctac ggcacgctcg
      901 tggacgcgga cgggtcgacg acggacctcg gcgccgacga cttctcggtc gaggtcacgg
      961 acgaatggac gagcccggcc accggggcga cgtaccccgc ggcctggcgc gtccgggtcc
     1021 cgtcgcaggc cctggagatc gacctccggc cgacggtggc ccagcaggag ctggacaccc
     1081 gggcgacgac gggggtcgtg tactgggagg gcagccaggt cgtgacggcg acgcgcgacg
     1141 gacgacgact gggtggtcag gcctacgtgg agctcacggg gtacgcgaaa ccctagccca
     1201 gggtgcgtcg ccactcggcg ccgtccggat agtggaacgc ggccagggtc tcggccttga
     1261 tctccgccga atacccgggc gtcgtcggca gcaggtagga gccttgccgg atcaagaccg
     1321 gatcgacgaa gtgttcgtgg aggtggtcga cgtactcgat catccgcccg tcgagcgatc
     1381 cgctgaccgc gatgtaatcg aacgccgcca ggtgctggac gagctcgcac agcccgacgc
     1441 cgcccgcgtg gggacacacc ggcaccccga acttcgccgc cagcagcagc accgcgatca
     1501 cctcgttgac gccgccgaga cggcaggcgt cgatctggca cacgtccatc gccccggccg
     1561 cgaggaactg cttgaacatg atccggttct ggacgtgctc gccggtcgcg acgcggatcg
     1621 gcgccacggc acgggcgatc gccgcgtggc ccaggacgtc gtccgggctc gtgggctcct
     1681 cgatccagta cgggtcgaac ggccgcagcc gctccatcca gctgatcgct gagccgacgt
     1741 cccagcgctg gttggcgtcg accgcgagca tccgctccgc cccgagctcc tcgcgcagga
     1801 tcgtcgcccg gcgcacgtcg tcctcgacgt ccgcccccac cttcatcttg aaccgcgtcc
     1861 agccgtcagc cagggcttcg cgcgcgagcc gccggatctt ggcgtcgtcg tagccgagcc
     1921 agcccacgga cgtcgtggag gccgggtagc ccccggccag cagctccgcg gtgcgcgcct
     1981 cgcgtcccgg cgcggcggcc tcgagcaggg ccagcgcctc atcgggcgtc agcgcgtcgg
     2041 tgaggtagcg aaagtccacc agcgcgacga tctcggccgg cgtcatgtcc gcgagcagcc
     2101 gccacacggg cttgccggcc cgcttcgcga caaggtccca ggccccgttg acgaccgcgg
     2161 ccgtcgcgag gtggatcgcc cccttctccg gcccaatcca acgcagctgg ctgtcggcca
     2221 ccatcgcccg ccagaacgcg ccgaggtcgg cctcgatccc ctccacgtcg agcccgacga
     2281 tcagcggccg cagcgcctcg acggccgcga ccacgacctc ggtcccccgg ccgatcgtga
     2341 acgtgaaccc gtggccctcg agcccgtccg cggcatcggt ccgcaggatc acgtacgccg
     2401 ccgaatagtc ggggtcgggg ttcatggcgt ccgagccgtc gagcgagcgc gacgtcggga
     2461 agcgcaggtc gatgacgtcg aggcccgtga tccgtgtcac ctgttcaccc cttgagggcg
     2521 ccgaccagca ggccgcgcgt gatgaatcgc tccagcgcga tcgccatcac gacgaccggg
     2581 gcaatcatga tcaggatcag caccgacatc gcccaccact gtggaccacg ggtggcgttc
     2641 tgtgcggcga cctggagcgg catggtctgg gcgtcggcgc tggtgaggac gagggcgatc
     2701 aggaactcgt tccaggagaa gatcagcacg atcaggaacg tcgccacgag accgggcacc
     2761 gagatcggga gcacgatcga gcgcacgatc cggtagatcg acgccccgtc gaccgccgcc
     2821 gactcctcga gctcacgcgg cagcgacatg aagtagtcgc gcatcagcca cacgacgatc
     2881 gggatgttgg tggcgacgta ggtgacggtg agggcggcga tgttgtcgac caggccgacc
     2941 tgctggaaaa gcacgtacag cggcacggcg accgcgatcg gcggcaggat ccgttgagag
     3001 atcagccaga acgcgatgtc gctgttcgcc agcgcccagg tgaaccgccg gccgatggtc
     3061 tgcgccagga tcaggaacac ggcgaaggcc gcggccagca tcagcgccgg cggcccgccg
     3121 agggccatcc cgacgaacgc cagcacgagg cagccgatga acaggccgat gagcgccacc
     3181 ttcggccggt aggtgaaccg aaccagcgca tacgccgtga ccgagcccag cagcagggcg
     3241 atcaacgagc tggacaggcc gacgatcacg gtgttcacgt acggccggag cgtgtcgttg
     3301 cccagctcaa ccaggatgta gcgccaggca tcgagcgtcg gcacgaagtc gacgaacggg
     3361 atgtagaacg ggcccttgtc cacctggtac ggcgtcttga acgaggtgac gaacagccag
     3421 tagatcggga acaggaccac gaacgtccag cccagcagca tcagccaggc gacgaacttg
     3481 gcgggcggtg agaggtcggt cgtggaccgg cggcggaacg ggttctgcat cgctcagacc
     3541 accgtcgtca cgcgtcgccg gacgtagctc acgtagaccg tcgcgacgat cgtgaccacg
     3601 atcagcagca ggtaggcgac cgcggcgctc cgcccgaggt tgagcgtgtt ccagtcgatc
     3661 cacgcctgga gcgtcagcga ctgggtggct gtgcccgggc ccccgcccag caggatgttg
     3721 ggcatgtcga tgatcttgaa gccctcgatc aggcggatca gcacgatggt cgtcgtcacc
     3781 ggcaggaggg ccggcatggt gatgtgcagg aacgactgcc agcggctggc gccgtcgacc
     3841 gtcgccgcct cgctgacctc ctggtcgagc gactcgaggg cagccagcag cacgatgaac
     3901 acgaacgggg tccactgcca cgtgtcgccg atgatcacgg ccgcgcgtgc gagccacggg
     3961 tcggtcaccc aggtgaagtc gcgcaggccg agcgagaccc acagcggctc gaggggaccc
     4021 ttcgaggtgt cggtcatcat caggaacatg tagccgacgc cgaccggcgt gatcgtgagc
     4081 gggatcagga acacgacccg gaagaaccgg cgccagggca cctgctggac cgccagcagc
     4141 gccagcccca ggccgagccc cacctggagg gcgatcccgg cgaacacgaa gatgagggtc
     4201 acgatcagtg agcccggccg gccgccctcg ctgaacagcg cctgcacgag caggtagacg
     4261 aaaccgacga gcaggacgcc cgcgaacagg cgaaggacga gcccgaaggg gcccgtctgg
     4321 ccgctccgcg cggcccgtgt ccaggcgatc gtgaccagca cgatcgtgag cccgacgatg
     4381 atccagccga gggggttggg cggcttgagc acgcccagga agtggctgcg ctcgaggccg
     4441 aacagcagct gctggtagtt ggcgaagccg acgaacttga gctcgacgcc gccccggttg
     4501 aacgcgagcc gcgagagcga catcacgacc gacgcgatca gcgggaacag cgacaggctg
     4561 aggatgacga tgacggtggg ccagatgaac agccatctgg ccgcgccgtc ggaacgccag
     4621 gcgccgagcg tcgtggacgc ccggctcctg gcggcttcga tctggctagc gctggacgcc
     4681 aaggctcgcg acgtacgcag cgagctggtc gttcttgccc acctcgtcgg tgatggcgtt
     4741 ccagccatcg gcgatggcct gcccggtgcc ggcctcgtcg agctcgccgg ccaggtactg
     4801 ggcgacggcg gtgtcgagga cgtcctgctg gtaccgcttc gtctgcggga tccgcatgtc
     4861 gagcaccatg ttcttgttct cgagactgga cttgatggcg ccaagatagt tggtcgcggc
     4921 agcctcgctg aggccggcgg cgacccacgg cgcgaggttg ctgaagtgcg acgtccggta
     4981 cgggttgtag ccggtcttgc cgagcgtcac atcctcaccg gagatcgccg gggaggccat
     5041 gtacgacagg aagtcgtaca ccgcgtcctg gttggccgcc ggcgatgccg cattgatggc
     5101 gcccgaccag ccgccgaacg aggcgaacgg cgcgtagttc acgccgtcga ccgcgtcggg
     5161 gcaggtcgtc gcgtcgcagg cgacgagctt gccgctggcg cggtcgagga cctgcgtcca
     5221 gccgggggtg atggtggcac ccgtcttgtc ctgggcgtac gtgcccggcg tcagggttcc
     5281 gatgtcgccc cagtccatcg tcagcgcgca gcgaccggtg gtgaacaggc cacgcgtgcc
     5341 acccacgtcc atgttgagct cgtccggcgg accgaactcg gcggtcttct tgtacgtctg
     5401 gagggccttg gtcatggcct cgttgggtcc gaacagcggg ttcatgttcg tcgtgtcgaa
     5461 gaacgcgccg tcgccggtgc ccttgccctg gagcagcccg gccgcgatgg agatgatcca
     5521 ccagtagctc tgggcgccct tcttcttggc gatgcacgag ccgtagtccg gctcgccgtc
     5581 gccgttgagg tcctggccct ggtacttctg cgcgaccgcg aggtagtcgt cccaggtctt
     5641 ggggggcgcg accccgtcct tctcgaggat gtcgctccgg taatagacca tgtggaagtc
     5701 gccgtcgagc gggatcgtgt agaccttgcc gttgtacgtg gcgttgaagt cacggaagaa
     5761 cggcccgatg tcctgccagt cgagctgcgc gtcggcgttg acgcggtcgg tcaggtcggc
     5821 gagatagccg gggcccgtga agtcgcccag ccactggggg tcgaacacgt aggcgtcgaa
     5881 ggcgttggtg ccggtcgacg cgtcgagcag tgccttgtcg tagatcgtct ggaagccgac
     5941 cgcgacgacg ttgacgtgac cgccggtcag gcacgcccaa tcgggagcgc ggcgctgcag
     6001 cggctccgcg acctgcggac cattgaacgt gaggatgttg acgttcgctc ccgcgaactt
     6061 gccgtccgtc gcgcaggccg ccggcgtgac cgccgcctcg gacggcgcgg cttcggatgc
     6121 cgcctccgtg ggggccgcga cggacgctgg cgccgccgtt gccggggtgg atgcgacggg
     6181 cgccgtggtc ggggattggg cggcacagcc cgccaggacg agaaccaagc cgaccgcggg
     6241 cgcgatcgat ctgaaccgcg acacgcactt cctcctcttg acccccgccc ggttcgtcca
     6301 gcatactgaa cgaccggcgg taggttgaac agtatgggga ccgcccgcgt cgcaagtcaa
     6361 tatgttgtac gcggtccatc ctgctggccg accgcacaca cgccgcgggc cagcgccccc
     6421 gggatcggag ccgcacgatg agcaacgaga ccctcgaacg ggacgcggag gccgagtcgc
     6481 tcgcgcccgc ggtgacccgt gcggcgctca tcctcggcgt catggccgag acggcctcgg
     6541 agccggtcgg acccagcgag ctcgcccgcc gcctgggact cccgaagtcg tcgatcgcca
     6601 acatctgcgg cgcgctcgcc gatgcaggcc tggtccggcg gatcgggacg gggttcgcgc
     6661 tggggcgccg gctcgccgag ctgggcggcg cctacctcgg gtccgtggac caggtccagg
     6721 agttctacga cgtcgcccgc cagctggcga cggcctcgga ggagacggtc cagttcgcgg
     6781 tgctcgacgg gctcgaggtg acctacctcg cccgccacga cgggcgccag ccggtgcgcc
     6841 tcacgtcggg catcggccgc cgcctgccag cgttctcgac cgcgaccggc aaggccgcgc
     6901 tggcgtcact ccccgacgac gagctcgacc ggcgcctggc cggactcacg acgctccccc
     6961 agccgtcacc caagtcgcac gcgacggtgg actcgctgcg ccgggacctc gcaacgatcc
     7021 gcgagcgcgg ctacgcgatg gacgacgagg agaccatgga gggcgtcgtg tgcttcggga
     7081 ccatgatccc gtcgcgccag cccggcgagg gaccgtgcgc cgcgagcatc acgctgctca
     7141 aggtcagggc gacgtccgag cgcgtgccgg ccctgctcga tgacctgcgg ctgctggccg
     7201 ccgagctctc ggacccgct
//
LOCUS       DBNHXO010000444         2657 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1723439, whole genome shotgun sequence.
ACCESSION   DBNHXO010000444 DBNHXO010000000
VERSION     DBNHXO010000444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1723439"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..839
                     /locus_tag="AC162A_08950"
     CDS             51..839
                     /locus_tag="AC162A_08950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5011009.1"
                     /translation="MPADRWSDADAYERYVGRWSRRVADAFVAWLGLAADSSWVDVGC
                     GTGALSGTIAARSRPRRVLGIDPSPDFVAAARERVADPVASFRVGRADALPLESGEAD
                     AVVSGLVLNFVPDVPASLAEAVRVTRPGGTVAAYVWDYAGRMELMRRFWDAAVALDPG
                     ARELDEGVRFPLCAPDRLAAAWINAGLSNVLVMAIDVPTRFADFDDFWAPFTVDIAPA
                     PGYASRLPLDRREALRELLRTTLPTGPEGTIDLIARAWSIRGQA"
     gene            874..1215
                     /locus_tag="AC162A_08955"
     CDS             874..1215
                     /locus_tag="AC162A_08955"
                     /inference="COORDINATES: protein motif:HMM:NF015947.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR5011010.1"
                     /translation="MLSAKVSTKHQISLPSEARRRLGIEPGDRLDVRIEDDTLVLRKQ
                     PARASDRLRGLTAGKGYFEPDPVTYVRRLRDEFEENLRAREALVGRRPPQAADPDRDR
                     RERPDLPPGER"
     gene            1118..1597
                     /locus_tag="AC162A_08960"
     CDS             1118..1597
                     /locus_tag="AC162A_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR5011011.1"
                     /translation="MPEKRWSADDLHKRQTRIAIDANVLIYLLENAEPHASRAAVVID
                     AINQHQIGASLATVGQVEILTGPARSGDATTFERMADELRSLDLRLVGLSAAIAEDAA
                     WLRGTEGIDLVDAIHLASARSAGATAFVTNDRGIRSRAGLEVLYLDDLVLEDPKARG"
     gene            1664..>2657
                     /locus_tag="AC162A_08965"
     CDS             1664..>2657
                     /locus_tag="AC162A_08965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5011012.1"
                     /translation="MDLTLTPAQTDLRDRARAYVVDALQPLEEEFERNHALSDDTVRE
                     LKRLAIEARLHGGSLPVSAGGQGWTAIEQVLVHEQLGQATGGLWSFIPGAYNALVHCT
                     PEQRRRYLDPSLRGERNGSYAITEAGQGSDARTLASTAVHDAATGEWVLNGEKWFVTG
                     PADTDFMIFHCHVLEDGERKPTLFLVDYDAPGLAMTGDPDYTHTFADRHPQFTLTDVR
                     VTDDALLGGVGEADAMTNEWFVEERLHIGARCSGAMERLLTLAVEWATSRIQFDARIY
                     DFQGVSFPLADSAADASAARLLTRETAWLMDEGTDPKIVHARASVAKLFASEAAW"
BASE COUNT          372 a          984 c          925 g          376 t
ORIGIN      
        1 catgaccgag atccggatca tggtcctcgc ctggccccag tagcccgccg atgccggccg
       61 accggtggag tgacgccgac gcctacgagc gctacgtcgg ccgctggagc cggcgcgtcg
      121 ccgacgcatt cgtcgcctgg ctgggcctcg ccgccgactc gagctgggtc gacgtgggct
      181 gcggcaccgg ggcgctgtcg gggacgatcg ccgcgcggag ccggccgcgg cgcgtgctcg
      241 ggatcgaccc gtcgcccgac ttcgtcgcgg cggcgcgcga gcgcgtcgcc gatccggtcg
      301 cgtcgttccg ggttggacgc gccgacgcgc tgccgctcga gtccggcgag gccgacgccg
      361 tcgtctcggg cctggtcctg aacttcgtgc ccgacgtgcc ggcctcgctc gcggaggccg
      421 tccgcgtcac ccggccgggc gggacagtcg ccgcctacgt ctgggactac gccgggcgga
      481 tggagctgat gcgccgcttc tgggacgcgg ccgtcgcgct cgaccccgga gcgcgcgagc
      541 tcgacgaggg cgtgcggttc ccgctgtgcg cgcccgatcg cctcgcggcg gcctggatca
      601 acgcgggtct ctcgaacgtg ctggtgatgg cgatcgacgt ccccacccgc ttcgccgact
      661 tcgacgactt ctgggcgccg ttcacggtcg acatcgcgcc tgccccgggg tacgcgagcc
      721 ggctgccgct cgaccgccgc gaggcgctgc gcgagctcct ccgcaccacc ctcccgacgg
      781 gcccagaggg cacgatcgac ctcatcgcgc gcgcctggtc tatccgcggc caggcttgac
      841 gacccgggtc tcgctgctta gcatcgcggc aacatgctaa gcgcaaaggt cagcaccaag
      901 caccagatct cgctgccaag cgaggcccga cggcggctgg gcatcgagcc tggtgaccgc
      961 ctggacgtcc ggatcgagga cgacacgctg gttctgcgca aacaaccggc gagggcgtca
     1021 gatcgccttc gcgggctcac cgcgggcaag ggctacttcg agcccgaccc tgtcacctac
     1081 gtccgccgcc tccgcgacga gttcgaggag aacttgcgtg cccgagaagc gctggtcggc
     1141 cgacgacctc cacaagcggc agacccggat cgcgatcgac gcgaacgtcc tgatctacct
     1201 cctggagaac gctgagcccc acgcctcccg agccgcggtg gtgatcgatg ccatcaacca
     1261 gcaccagatc ggcgcttccc tggcgacggt cggccaggtc gagatcctga cgggtcctgc
     1321 gcgctccggc gacgcgacga cgttcgagcg catggcggac gagctgcgct cccttgacct
     1381 tcgcctcgtt ggtctgtcgg cagcgatcgc tgaggacgcg gcctggctgc ggggcaccga
     1441 ggggatcgac cttgtggacg cgattcacct cgccagcgcc cgcagcgccg gcgcgacggc
     1501 gttcgtgacg aacgatcggg gcatccgctc gcgcgccggc ctcgaggtgc tgtacctgga
     1561 cgacctcgtt ctcgaggatc cgaaagcccg aggctgagtc gcgtgtcccg ggcgccgcga
     1621 ccggctcggc cggcgtcgcc ggggtggccc acactgcgcg gcgatggacc tgacgctcac
     1681 ccccgcccag accgacctcc gcgatcgcgc ccgtgcctac gtcgtcgatg ccctccaacc
     1741 gctcgaggag gagttcgagc gcaaccacgc gctgtccgac gacaccgtcc gcgagctgaa
     1801 gcggctcgcg atcgaggcac ggctccacgg cggcagcctc ccggtatcgg ccggtgggca
     1861 gggctggacg gccatcgaac aggtgctcgt gcacgagcag ctgggccagg cgaccggcgg
     1921 cctgtggagc ttcatccccg gcgcctacaa cgcgctcgtc cactgcacgc ccgagcagcg
     1981 ccgccgctac ctcgatccca gcctgcgcgg cgagcgcaac ggcagctacg cgatcacgga
     2041 ggccggccag ggctccgacg cccggaccct cgcctccacg gcggtccacg acgcggccac
     2101 tggggagtgg gtgctcaacg gcgagaagtg gttcgtgacc gggcccgcgg acaccgactt
     2161 catgatcttc cactgccacg tgctcgagga cggcgagcgg aagcccacgc tgttcctcgt
     2221 cgactacgac gcgccggggc tcgccatgac cggtgatccc gactacaccc acacgttcgc
     2281 cgaccgccat ccgcagttca cgctgaccga tgtccgggtg accgacgatg cgctgcttgg
     2341 cggcgtgggc gaggcggacg cgatgaccaa cgagtggttc gtggaggagc ggctgcacat
     2401 cggagcccgc tgctccgggg cgatggagcg cctgctgacg ctggcggtgg agtgggcgac
     2461 gagccggatc cagttcgacg cgcggatcta cgacttccag ggcgtgagct tccccctcgc
     2521 cgactcggcg gccgacgcct ccgcggcccg cctgctgacc cgcgagaccg cgtggctcat
     2581 ggacgagggc accgacccca agatcgtcca cgcccgcgcc tcggtagcca agctgttcgc
     2641 gtccgaggcg gcgtggc
//
LOCUS       DBNHXO010000445         4034 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5098427, whole genome shotgun sequence.
ACCESSION   DBNHXO010000445 DBNHXO010000000
VERSION     DBNHXO010000445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5098427"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1388
                     /gene="ftsY"
                     /locus_tag="AC162A_08970"
     CDS             138..1388
                     /gene="ftsY"
                     /locus_tag="AC162A_08970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR5011013.1"
                     /translation="MRVLRRGPSEDEPEPQPADEPADAPSAEPEEAPASDAAPADDLI
                     GDEWRSADSPDDVVVPEPSALELDLAGEPDAILGQLPPVVPVVHAEPVVAPAPEPEVQ
                     WAWGADYSPEKADAARREREREQLEHGLEKSRTGFGARLRSALGGGDGADWEEIEEIL
                     ITGDVGAALAMDVVERARQRRDVSAEAAVRAELAALLAERDAEHWEPRPAVEGGPAIV
                     LVVGVNGTGKTTTIGKLAARERAQGRRVVLAAADTFRAAAIDQLRIWAQRTGSEIVAH
                     APNADPAAVVFDAIDAAVARHADIVIADTAGRLHTKSNLMDELAKVRRVIDKRLPGAA
                     VETFFVLDATTGQNGLAQALAFNAAVGLSGVVLTKLDSTAKGGIVFAIEHDLGVPVRF
                     VGVGEQADDLLPFDPDAFVEALFD"
     gene            1574..2908
                     /gene="ffh"
                     /locus_tag="AC162A_08975"
     CDS             1574..2908
                     /gene="ffh"
                     /locus_tag="AC162A_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR5011014.1"
                     /translation="MFDSLSDRLRQTLAGLTGRGRVSEADVDAAMREVRLALLEADVN
                     FKVVKEFVARVRERAVGAEILDSLTAGQQVVKIVHEELTALLSTGDRTFHLSGSPAVV
                     ALVGLQGSGKTTSAAKLAKHIAKLGRQPMLVAADPYRPAAADQLETLGKSLGIAVHRA
                     PVGTPTVDIARGGIEAAKRMGRDVVIIDTAGRLTIDDALMDEIRAINAAVKPVETLLV
                     VDAMTGQEAVTVAQGFAGAVPVTGLVLTKIDGDARGGAALSIGAVTGIPVKFLGTGEK
                     TDALEVFYPDRLSGRILGMGDIVTLTERAQEAFDEKQAAKLEESVRKGAFTLEDMLAS
                     MRQMQKMGPMGQVMSMIPGMGGMAKEAQDAVDRGDLKRVEAIILSMTPRERREPGVLN
                     ASRRRRIANGSGTTLPDVNRLIKQFAEMQKLMKQLSGAGGRRAAMGQMLGRR"
     gene            2923..>4034
                     /locus_tag="AC162A_08980"
     CDS             2923..>4034
                     /locus_tag="AC162A_08980"
                     /inference="COORDINATES: protein motif:HMM:NF023260.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HIR5011015.1"
                     /translation="MTDQDPTQAYVPPPGEPPVPPTPAVPEAPVAPTADVAPTAAYVP
                     PAPPAPDAAPPAAPIPTFAPPEAPAAGVVTAPVVAAPATKRRSPLKWVAALVVVALVA
                     GAAIAAAALLTGASGTPTVLAWAPADSVTYAEVRLDLPGNQQAELAKVVSAFPGFDDQ
                     AAFPTKINEVLDQLVGKASDGKQSYTADIQPWFGGQLSVSVGPVPSSSDASKARALAL
                     ASVTDAAKATAWAASTLAESGATSTTETYNGVTITVIQPKADVSVMAKEIQPAYAVTG
                     PVLALGDLASVKAAIDTAGKSGLNTNAQFKTASSTITGDRLAFAYVDTAAIVDAAGAM
                     APDIAGLPAMPSSLDGLFPAWLASAVVARDSALVVET"
BASE COUNT          595 a         1540 c         1379 g          520 t
ORIGIN      
        1 cggcgtcacc gtgggcgacg actcggtcag tcgcgtgatc agcctgcgcc tcgacgaggc
       61 ccaggccatc gccgatgatc gccggaccga ggcgcgcgcc gtcctcgccg gcgagcccgg
      121 ctgaaccgga gtccccgttg cgtgtcctcc gtcgcggccc gtcagaggac gaacccgagc
      181 cgcagccggc cgacgagccc gcggacgccc cgtccgccga gcccgaggag gcaccggcga
      241 gcgacgccgc acccgccgac gacctgatcg gcgatgagtg gcggtccgcc gactcacccg
      301 acgacgtcgt cgtgccggag ccgtctgcgc tggagctaga cctcgctggc gagccggacg
      361 ccatcctcgg ccagctgccc ccggtggtgc cggtggtcca cgccgagccc gtcgtcgctc
      421 ccgcccccga gcccgaggtc cagtgggcct ggggcgccga ctacagcccg gagaaggccg
      481 atgccgcccg gcgcgagcgc gagcgcgagc agctcgaaca cggcctcgag aagtcgcgga
      541 ccgggttcgg cgcccgcctg cggagcgcgc tcggcggtgg cgatggcgcc gactgggagg
      601 agatcgagga gatcctgatc accggcgacg tcggcgccgc cctcgcgatg gacgtcgtcg
      661 agcgggcgcg ccagcgtcgt gacgtgtccg ccgaggcggc cgtccgcgcg gagctcgccg
      721 ccctcctggc ggagcgggac gcggagcact gggagcccag gccggccgtc gagggtggcc
      781 cggcgatcgt gctggtggtc ggcgtcaacg ggaccggcaa gaccacgacc atcggcaagc
      841 tcgcggcccg cgagcgcgcc cagggccgtc gcgtcgtcct cgcggccgcc gacacgtttc
      901 gcgcggccgc gatcgaccag cttcggatct gggcccagcg gacgggctcc gagatcgtcg
      961 cccacgcgcc caacgccgac ccggcggccg tggtcttcga cgccatcgac gccgcggtcg
     1021 cgcgccacgc ggacatcgtg atcgccgaca cggcgggtcg cctgcacacc aagtcgaacc
     1081 tcatggacga gctggccaag gtccgccggg tgatcgacaa gcgactgccg ggtgcagccg
     1141 tcgagacgtt cttcgtcctc gacgccacca ccggccagaa cggcctcgcg caggccctgg
     1201 cgttcaacgc tgccgttggg ctgtccggcg tcgtcctgac caagctcgat tcgacggcca
     1261 agggcggcat cgtattcgcg atcgagcacg acctcggcgt gcccgtccgg ttcgttgggg
     1321 tgggggagca ggccgacgac ctcctcccgt tcgacccgga cgccttcgtc gaggcgctgt
     1381 tcgactgacc ttccggccgc cgaccagggg gacggtcgtt gccagacggc ccacgaccat
     1441 cgggccaggg gcgaccttcg gggtcatgac gtacactccc accgcctcgg tgtcgccaga
     1501 acccgcgcac caaccggccg gtcttcgccg tccggcctga tctcaccgac agcacaaggg
     1561 tcgccgcctc cgcatgttcg acagcctcag cgatcgcctc cgccagaccc tcgccgggct
     1621 caccggtcgg ggccgcgtgt ccgaggccga cgtcgacgcc gcgatgcgcg aggtccgcct
     1681 ggccctgctc gaggcggacg tcaacttcaa ggtcgtcaag gagttcgtgg cgcgcgtgcg
     1741 cgagcgggcc gtcggcgccg agatcctgga cagcctgacc gccggccagc aggtggtgaa
     1801 gatcgtccac gaggagctca ccgcgctcct ctcgaccggc gaccgcacct tccatctctc
     1861 gggcagcccg gccgtggtcg cgctcgtggg cctccagggc tcgggcaaga cgacctcggc
     1921 ggccaagctt gccaagcaca tcgccaagct cggccgtcag ccgatgctcg tcgcggccga
     1981 cccgtaccgg ccggccgccg cggaccagct cgagacgctc ggcaagtcgc tcgggatcgc
     2041 ggtccatcgt gccccggtcg ggaccccaac ggtcgacatc gcccggggcg gcatcgaagc
     2101 cgcgaagcgg atgggtcggg acgtcgtgat catcgacacc gccggccgcc tcacgatcga
     2161 cgacgcgctg atggacgaga tccgcgcgat caacgcggcc gtcaagcccg tcgaaaccct
     2221 gctcgtcgtc gacgcgatga ccggccagga ggcggtcacc gtggcccagg ggttcgccgg
     2281 cgccgtcccg gtcacagggc tcgtcctgac caagatcgat ggcgacgccc gaggcggcgc
     2341 cgcgctgtcc atcggcgccg tgaccggcat cccggtcaag ttcctgggga caggcgagaa
     2401 gacggacgcg ctcgaggtgt tctatcccga tcgcctctcg ggccggatcc tcggcatggg
     2461 tgacatcgtc acgctcacgg agcgcgccca ggaggcgttc gacgagaagc aggccgccaa
     2521 gctcgaggag tcggtgcgca agggcgcctt cacgctcgag gacatgctgg cgtcgatgcg
     2581 ccagatgcag aagatggggc cgatgggcca ggtcatgtcg atgatcccgg gcatgggcgg
     2641 catggccaag gaagcccagg acgccgtcga tcgcggcgac ctcaagcggg tcgaggcgat
     2701 catcctgtcc atgacgccgc gcgaacgccg cgagcccggt gtgctcaacg cctcgcgccg
     2761 tcgacgaatt gccaatggat ccgggacgac gctcccggac gtcaatcgcc tgatcaagca
     2821 gttcgcagag atgcagaagc tgatgaagca actttccgga gcaggtggcc gtcgcgcagc
     2881 gatgggccag atgctcggcc gccgctagag ggacccaccg ccatgaccga ccaggatccg
     2941 acccaggcct acgtgccacc gcccggcgag ccgcccgtcc ccccgacgcc ggccgtgccc
     3001 gaggcgcccg tcgcccccac ggccgatgtg gcgccgaccg cggcctacgt cccgccggcc
     3061 ccgccggcac cggacgccgc cccgcccgcg gccccgattc cgaccttcgc cccgcccgag
     3121 gctcccgcgg ccggcgtggt caccgcgccc gtggtggcgg caccggcaac gaagcgccgc
     3181 agcccgctca agtgggttgc cgcgctcgtc gtcgtcgcgc tcgtcgccgg cgcggcaatc
     3241 gcggccgccg cgttgctgac cggcgcatcg ggcacgccca cggtcctcgc ctgggcacct
     3301 gccgacagcg tcacctatgc ggaggtccgt ctcgacctcc ccggcaatca gcaggccgaa
     3361 ctcgccaagg tcgtcagcgc gttccccggg ttcgacgatc aagctgcctt cccgaccaag
     3421 atcaacgagg tcctcgacca gctcgtcggc aaggcatcgg acggcaagca gagctacacg
     3481 gccgacatcc agccctggtt cggcggccag ctcagcgtga gcgtcggccc cgtgccctcg
     3541 agctccgacg cgtcgaaggc ccgcgcgctg gccctggcca gcgtcacgga cgccgccaag
     3601 gcgaccgcct gggccgcatc cacgctcgcg gagtccggtg ccacctccac gaccgagacc
     3661 tacaacggcg tcacgatcac ggtgatccag ccgaaggctg acgtcagcgt catggccaag
     3721 gagatccagc cggcgtacgc cgtcacgggc cccgttctcg ccctgggcga cctcgcgtcg
     3781 gtcaaggccg cgatcgacac cgctggcaag tccggcctca acaccaacgc ccagttcaag
     3841 acggcgtcgt cgaccatcac cggcgaccgc cttgccttcg cctacgtcga cacggccgcg
     3901 atcgtcgacg ccgccggcgc catggcgccc gacatcgccg gcctgcccgc gatgccctcg
     3961 tccctcgacg gcctcttccc ggcctggctg gccagcgcgg tcgtcgcccg ggacagcgcg
     4021 ctcgtcgtcg agac
//
LOCUS       DBNHXO010000446         3859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1131020, whole genome shotgun sequence.
ACCESSION   DBNHXO010000446 DBNHXO010000000
VERSION     DBNHXO010000446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1131020"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="AC162A_08985"
     CDS             <1..878
                     /locus_tag="AC162A_08985"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR5011016.1"
                     /translation="DGEVRDMTDAILATARKANLHALLCLGGGGTAKNALRVSKAGLN
                     VVMVPKTIDNDLACTDATIGFTTALEVATDASDRLHSTAHSHHRIIVAELMGHGAGWL
                     ALGAGLAGGADVILIPEIPYSVAAISAAIKERARRGTNFSIVAVAEGARSVEDAAALA
                     VAEGRADRAKEPGAREAAKAEVELLETQHIGNTLRLAHQLEQLTGLESRVSILGYIQR
                     GGTPSPVDRLLATRLGSAAADLVEAGTFGVMVAARGDETEPVPLDQVAGQRKRVPPDH
                     PWVAAARHVGTCLGD"
     gene            1013..1405
                     /locus_tag="AC162A_08990"
     CDS             1013..1405
                     /locus_tag="AC162A_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011017.1"
                     /translation="MRGLFTLILATIAALVGGVIGFQLGITSDIGAAGGTVVWHAGFP
                     FFGFIFPFLFFFLFIGLLFAAFGGRRRGWGGPGWGHGYGPNGYGPMGNADDPRRQWIA
                     DAHRRLHEDEARGAGTPPATPPTAPPSA"
     gene            1476..2162
                     /locus_tag="AC162A_08995"
     CDS             1476..2162
                     /locus_tag="AC162A_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5011018.1"
                     /translation="MRTILVIEDEPQIASLVRDYLEHAGFAVLAAGDGSGGLALARAR
                     RPDAIVLDLGLPRIDGLDVVRSLRRDSMVPIVILTARGDEVDRITGLELGADDYVVKP
                     FSPKELVARVRAVLRRADARSTADERIVAGDLELDVARHRVTAAGKRVTLTPTEFELL
                     ATLAREPGRVYTRSQLLDAVHGFSLESYERAIDGHIRNLRRKLAAASSSARYVRTVHG
                     VGYALEEPAG"
     gene            2285..3295
                     /locus_tag="AC162A_09000"
     CDS             2285..3295
                     /locus_tag="AC162A_09000"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR5011019.1"
                     /translation="MGAGFVGCLFLALVVFVGVLTALATWVAALLLGIIAPGASPPTA
                     TVVAVVVVLIVAVVIGGRAFFLTVRPLADLAAASERLADGEPGVRVETRGPRPVRRLG
                     ASFNAMAERLDRSRDDRRALLADVTHELRTPLTVISGGLEAMLDGVHPMDEDHVSPIL
                     AEAVVMDRLLDDLRTISLAEAGALPLHREPVDLVEIAGDVLAGQHAVADARRVELRLE
                     GDASLVADVDPVRVREILVNLVANAVRHTPAGGHVMIEVRADGGDALLTVVDTGEGIP
                     PEELDRVFDRFHRRSDSGGSGLGLTIVRDLATAHGGTVEVESDGVPGHGSAFRVRLPR
                     RL"
     gene            complement(3308..>3859)
                     /locus_tag="AC162A_09005"
     CDS             complement(3308..>3859)
                     /locus_tag="AC162A_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011020.1"
                     /translation="LGTGLAAIGLAGLLLGTPGSVLPAVEGSAGAAAGAAPSAPAAER
                     AEDGSVPMIDAPAPAAGAGLAEAAASEPAAMAPEVAPAPSAAAAVEPDRAFLTETGSG
                     AAEGRSSTTSIQQGPPQGGGEPAGTDRSLEAPEGDNVRQIAPSDAAAVQPAGGPAIAL
                     LGVAALIAGVVLVALRLGAKRLA"
BASE COUNT          479 a         1473 c         1405 g          502 t
ORIGIN      
        1 tcgacggcga ggtccgggac atgaccgacg cgatcctcgc gaccgcccgc aaggcgaacc
       61 tgcacgcgct cctgtgcctg ggcggcggcg gcacggccaa gaacgcgctg cgcgtctcga
      121 aggccggcct caacgtcgtg atggtgccca agaccatcga caacgacctc gcctgcaccg
      181 acgcgacgat cggcttcacc acggcgctcg aggtcgccac cgacgccagc gaccggttgc
      241 acagcacggc ccacagccac caccggatca tcgtcgccga gctgatgggc cacggcgcgg
      301 gctggctcgc gctcggcgcg ggactcgcgg gcggtgcgga cgtgatcctg atccccgaga
      361 tcccgtacag cgtcgcggcg atttcggcgg cgatcaagga acgcgcgcgc cgcgggacca
      421 acttcagcat cgtggccgtc gccgaggggg cgcggagcgt cgaggacgcg gcagcgctgg
      481 ccgtggcgga ggggcgtgcg gaccgggcga aggagccggg cgcccgcgag gccgccaagg
      541 ccgaggtcga gctgctggaa acccagcaca tcggcaacac gctccggctc gcccaccagc
      601 tcgagcagct cacgggcctc gagtcgaggg tgtcgatcct gggctacatc cagcggggcg
      661 gcacgccgtc gcccgtggac cgcctgctcg cgacgcgcct cgggagcgcc gcggccgacc
      721 tggtcgaagc cggcacattc ggcgtgatgg tcgcggcgcg gggcgacgag acggagcccg
      781 ttccgttgga ccaggtagca ggccagcgca agcgggttcc gccggatcac ccgtgggtcg
      841 ccgccgcccg ccacgtcggg acctgcctcg gcgactgagc gccgcagcga tctgcgcgaa
      901 catcgcaccg gttgcgcacg ggttctgcac atccgcccgc gaggctcgaa ggcatcaagc
      961 aaccacggcg ccggcccctg ccggcaccac ccttcgatcg gagcacttcc ccatgcgtgg
     1021 gctgttcacc ctcatcctcg ccaccatcgc cgccctcgtc ggcggcgtga tcggcttcca
     1081 gctgggcatc acgtccgaca tcggcgcggc cggcggaacg gtcgtctggc acgccggctt
     1141 cccgttcttc gggttcatct tcccgttcct gttcttcttc ctgttcatcg gcctgctgtt
     1201 cgccgcgttc ggcgggcgcc ggcgcggctg gggcggtccc ggctggggcc acggctacgg
     1261 cccgaacggc tacggcccga tgggcaacgc tgacgacccc cgtcgccaat ggatcgcgga
     1321 cgcccaccgc cggctccacg aggacgaggc tcgcggcgcg ggcacgccgc cggcaacgcc
     1381 gcccacggca ccgccaagcg cttgacgcac aatcccatcc agcggcgccg ggacggtacc
     1441 gtccgcccgg cgcctcgcca tttccggagt cactcttgcg cacgatcctc gtcatcgagg
     1501 acgagcccca gatcgcctcg ctcgtccgcg actatctcga acacgcgggc ttcgccgtcc
     1561 tcgccgcggg cgacggctcg ggcggcctcg cgctcgcccg tgcgcgccgc ccggacgcga
     1621 tcgtcctcga cctcggcctg cccaggatcg acggcctcga cgtcgtccga tcgctccgca
     1681 gggactcgat ggtcccgatc gtgatcctca cggcgcgtgg cgacgaggtc gaccggatca
     1741 cgggcctcga gctcggcgcg gacgactacg tggtcaagcc gttcagcccc aaggagctgg
     1801 tcgcgcgggt ccgcgcggtc ctgcggcgcg ccgatgcgcg ctcgaccgcg gacgagcgga
     1861 tcgtcgccgg cgacctcgag ctggacgtgg cccgccaccg ggtcacggcc gccggcaagc
     1921 gcgtcaccct cacgccgacc gagttcgagc tgctcgcgac cctggcccgc gaacccgggc
     1981 gcgtgtacac ccgctcgcag ctcctcgacg ccgtccacgg gttctcgctc gagtcctacg
     2041 agcgggcgat cgacggccac atccgcaacc tgcgccggaa gctggccgcg gcttcgagct
     2101 ccgctcgcta cgtccggacc gtgcacggcg tgggctacgc gctcgaggag ccggccgggt
     2161 gacgagcgcg gatccggacc cgcacgagcg ccaccgctgg ggcgcctcgg ggcgggagcg
     2221 gtggcaatgg ggcggaccac cgggccctgg ggggccgtgg gggcggcgtc ggggcccggg
     2281 ccgcatgggc gcgggcttcg ttggctgcct gttcctggcc ctcgtcgtgt tcgtcggcgt
     2341 cctcaccgcg ctggcgacgt gggtcgcggc gctcctgctc gggatcatcg cgccaggcgc
     2401 ctcaccgccc accgccaccg tcgtcgccgt cgtcgtcgtg ctcatcgtcg ccgtcgtcat
     2461 tggcggtcgc gcgttcttcc tgaccgtgcg cccgctcgcg gaccttgcgg cggcctcgga
     2521 gcggctcgcc gacggtgagc ccggcgtccg cgtcgaaacc cgcggtccga ggccggtccg
     2581 ccgcctgggt gcctcgttca acgccatggc ggagcgcctc gaccgctccc gcgacgaccg
     2641 ccgcgcgctg ctcgccgacg tgacccacga gctgcggacg cccctcaccg tgatctccgg
     2701 cggcctcgag gccatgctcg acggcgtcca cccgatggac gaggaccacg tttcaccgat
     2761 cctcgccgag gccgtcgtga tggaccggct gctcgacgac ctgcggacga tctcgctcgc
     2821 cgaggcgggc gcgctgccgc tgcaccggga gccggtcgac ctggtcgaga tcgccggcga
     2881 cgtgctggcc gggcagcacg cggtcgcgga cgcccggcgg gtggagctgc ggctggaggg
     2941 cgacgcgtcg ctcgttgccg acgtcgaccc ggtacgcgtg cgcgagatcc tggtcaacct
     3001 cgtcgcgaac gccgtgcggc acacgcccgc gggcggtcac gtcatgatcg aggtccgcgc
     3061 ggacggcggc gacgcactgc tgaccgtcgt ggacaccggc gaggggatcc cgcccgagga
     3121 gctggaccgg gtgttcgacc ggttccatcg gcgctcggac tccggcggca gcggcctggg
     3181 cctgacgatc gtccgggacc tcgcgaccgc ccacggcggg acggtcgagg tggagagcga
     3241 cggcgtgccg ggccacgggt cggcgttccg ggtgcgactg ccccgccgac tctaggcgcg
     3301 caaggcgtca ggcgaggcgc ttggccccca accggagcgc gaccagcacg acgcccgcga
     3361 tcagggcggc gaccccgagg agggcgatgg ccgggccccc ggcaggctgg accgcggccg
     3421 catcgctcgg tgcgatctgg cgcacgttgt cgccctccgg cgcttcgagg ctgcggtcgg
     3481 tgcccgcggg ctcgccgccg ccctggggcg gtccctgctg gatcgacgtg gtggacgagc
     3541 ggccctcggc ggcgcccgac cccgtctcgg tcaggaacgc ccggtcgggc tcgaccgcgg
     3601 cggcggccga cggagccgga gcgacctcgg gcgccatggc tgcaggctcg gaggcagccg
     3661 cctcggccaa ccccgcgccg gccgcgggcg ccggggcgtc gatcatcggg acgctgccat
     3721 cctcggccct ctccgcggcc ggggcgctcg gcgcggcacc ggcggccgcg cctgccgaac
     3781 cttcgacggc cggcaggacc gagcccgggg tccccaggag cagcccggca aggccgatgg
     3841 cggcgagccc ggtgcccag
//
LOCUS       DBNHXO010000447         2282 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4105917, whole genome shotgun sequence.
ACCESSION   DBNHXO010000447 DBNHXO010000000
VERSION     DBNHXO010000447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4105917"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="AC162A_09010"
     CDS             <1..419
                     /locus_tag="AC162A_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5011021.1"
                     /translation="QERVPLEAAGDVFVIGADGTGERNVTSSTAFEQDPAWSPDGTRL
                     AYLSSEDGENYRLTTLPMEGAMPAGRPTLGPESEFAVWSPDATQLLWVGGGQSASGEG
                     VSLIQVIDRDLAKPATAIASVAGLILCAPSWQSASR"
     gene            531..1610
                     /locus_tag="AC162A_09015"
     CDS             531..1610
                     /locus_tag="AC162A_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5011022.1"
                     /translation="MDRRLLLALVAALAVAACGSAPAAPAPTGTSTPAPQLEPTPAPT
                     DAPSPTDAPTAAAASEPPIALPSTGSFVFTRYDGDRSSLWVSCADLSEARQLPAPSGL
                     DADYGVWSPDGQRIVFNGGRNDPDVNDAVEPWDIYTMDRDGGDIRKLTDATALLGDPV
                     YSPDGKLITYDSTEAGRDGIWVMDADDGGNKRLVAPLPDGLLQDYAPTFSPDGKRIVF
                     LREVDAENEAALWIVNLDGSDLHRLTEPGLYPNVSEWSPDGSRIVFDAFRPGEELSTI
                     WMIDADGQHLTELTPPDPVAAGRDGYSKPTWSDDGSLVYTIHGIHPGPTRLGIAVMRP
                     DGTGLRYVADGSGYEHQPDWTDTPC"
     gene            1665..>2282
                     /locus_tag="AC162A_09020"
     CDS             1665..>2282
                     /locus_tag="AC162A_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011023.1"
                     /translation="MSEPSQPAVDAPSHAPRFLLLNALFPGLGHLAAGRLQWALLLGG
                     PILVLLLVMVVIALTTNATSVAARMFDPAVLTALLGIQVLILGWRLFAVGATKVITPI
                     TSRAATVAALALSLIIVIGPQLVVAGLTVDARDAAEDVFASVGDGGAWVPTETAPPVE
                     PNDPDFAIDPSASPGASLEPSTSPTATPEVPRINVLLIGMDSGVGR"
BASE COUNT          335 a          860 c          763 g          324 t
ORIGIN      
        1 agcaggagcg cgtgccgctg gaggcggccg gggacgtctt cgtgatcggg gccgacggga
       61 cgggagagcg gaatgtcacg agctcgacgg ccttcgaaca ggacccggcc tggtcgccgg
      121 acggcacccg gctcgcgtat ctcagctcgg aggacggcga gaactaccgg ctgaccacgc
      181 tgccgatgga aggtgcgatg cccgccggcc gacccaccct cggaccggag tcggagttcg
      241 cggtctggtc gccggacgcg acccagctcc tgtgggtcgg gggcgggcaa tcggcatccg
      301 gcgaaggcgt gagcttgatc caggtgatcg atcgcgacct cgcgaagccg gcgacggcga
      361 tcgcctccgt cgccggcctg atcctgtgcg ccccgagctg gcagagcgcc agccgctgag
      421 cgcatcgccg gccggtcgtt ccgccgaatc ggaacaatcc cgtcacgagc cggcccagac
      481 caagtagcgc ggccgccagg gccgccgccc tcgagaggcg atacgccgac atggaccgac
      541 gtttactgct cgccttggta gccgccctgg cggtggccgc gtgcggctca gcgcccgccg
      601 cgccggctcc gaccgggacg tcgacaccag ctcctcaact cgagcccacg ccggccccaa
      661 ccgacgcgcc gtcgccaaca gacgcgccca ctgccgcggc agcaagcgag cctccgatcg
      721 cgctgccctc gaccggctcg ttcgtgttca cgcggtacga cggggatcgc agttcgctct
      781 gggtctcgtg cgcggacctc agcgaagctc gccagcttcc cgcccccagc ggactcgacg
      841 ccgactacgg agtctggtcc cccgacgggc agcgcatcgt gttcaacggc ggccgcaacg
      901 accccgacgt caacgatgcc gtggagccgt gggacatcta cacgatggac cgggacggcg
      961 gcgacatccg gaagctcacc gacgcaaccg cgctcctcgg cgaccccgtg tactcacccg
     1021 acggcaagct gatcacgtac gactccaccg aggccggccg ggacggcatc tgggtgatgg
     1081 acgccgatga cggcggcaac aagcgcctgg tcgcgccgct gcccgatggc ctgctccagg
     1141 actacgcgcc gacgttctcg cccgacggca agcgcatcgt gttcctgcgc gaggtcgacg
     1201 ccgagaacga ggcggcgctg tggatcgtca acctcgacgg cagcgacctg catcggctca
     1261 cggagccggg cctgtatccg aatgtctctg agtggtcgcc ggacggttca cgcatcgtgt
     1321 tcgatgcctt ccggcccggc gaggaactgt cgacgatctg gatgatcgac gccgacgggc
     1381 agcacctgac cgagctcacg cctccagatc ccgttgcggc cggccgggac ggctactcca
     1441 agccgacgtg gtcggacgac ggctcgctcg tttatacgat ccacggcatc catcccgggc
     1501 cgacgcgcct cggcatcgcg gtcatgcgcc ccgatggcac gggcctgcgc tacgtggccg
     1561 acggcagcgg ctacgagcac cagccggact ggacggacac gccctgctga cccgctgaac
     1621 cccgcggttg cggcgaatcc ggatcgaccg catccttgcg gccaatgtcc gaaccgtcac
     1681 agccggccgt cgacgccccg tcgcacgcgc ctcgcttcct gctgctcaac gccctgttcc
     1741 cgggcctcgg ccacctcgcg gcaggccgcc tccaatgggc gctcctgctc ggcggcccga
     1801 tcctcgtgct gctcctcgtc atggtcgtca tcgccctgac gacgaacgcg acgtcggtcg
     1861 ccgcgcgcat gttcgacccg gcggtcctga ccgcactgct cggcatccag gtgctgatcc
     1921 tcggctggcg gctgttcgcg gtcggggcga ccaaggtgat cacgccgatc acgagccgtg
     1981 cggcgaccgt cgccgcgctc gcgctgtcgc tgatcatcgt catcgggccg cagctggtcg
     2041 tcgccggcct gaccgtcgat gcccgcgacg ccgccgagga cgtgttcgcc tccgtcggcg
     2101 acggcggtgc ctgggtgccc accgagacgg cgccgccggt cgagcccaac gaccccgact
     2161 tcgccatcga cccgtccgca tcgcccggcg cctcgctcga gccgagcacg tcgccgacgg
     2221 ccacgcccga ggtcccgcgc atcaacgtgc tgctgatcgg catggactcg ggcgtcggcc
     2281 gg
//
LOCUS       DBNHXO010000448         2204 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1132090, whole genome shotgun sequence.
ACCESSION   DBNHXO010000448 DBNHXO010000000
VERSION     DBNHXO010000448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1132090"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="AC162A_09025"
     CDS             complement(<1..747)
                     /locus_tag="AC162A_09025"
                     /inference="COORDINATES: protein motif:HMM:NF014646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR5011024.1"
                     /translation="MYTIKEASARSGVGAPLIRAWERRYGVIRPVRTPSGYRLYDDGT
                     IRVLVAMQSLVASGWTASEAARAIGSGEVRLDDVVPVARPTLASGRPSPGPDHRQQLI
                     ARFVRAAEANSAPDTESVLDEMLASGSFESIVDDLLLPAAAALGDAWAEGRLSVAGEH
                     AASGAMGRRLAAAFQAAGAPRKVSVVVGLPPGSRHELGALAFAAALRRRGIGVLYLGS
                     DVTVDGWVDALARTRAKAAVVGVITDDDRES"
     gene            809..2107
                     /locus_tag="AC162A_09030"
     CDS             809..2107
                     /locus_tag="AC162A_09030"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5011025.1"
                     /translation="MTDPPPTPRRRFGGLLVDPAPLRLDRDFRLLWIGQGISTVGRTF
                     TLVAVPYQVYVLTGDILAVGALSVVQLAATLIFALGGGAVADAVDRRRLLLFTQVGQA
                     GCSAAFLLIALSPSPSLLAIFAVMFVSMGLGAIDGPARASAVPRLVPIERLQAAIGLN
                     QLMFNAAWVIGPALGGIVLATAGPAAAYAIDIVTFAAVIVALLLISPIPPAPDAVRPS
                     IGAVVEGLRFARRRREVLATFIVDLNAMIFGSPASLYPALALDVFMVGPAGVGLMWSA
                     TGFGAMVGALFSGWTTTVRRTGRAVLIAVAVWGLGILGFGLATFSFPLALLFLAIAAG
                     ADVISAVLRSSIVQILTPDTLRGRVSSIHILVVTAGPELGNAEASVVASVIGTQGAVV
                     SGGLLTLAGLGAIALAMPEFRRLDMPTAIADTAAAAHAVG"
BASE COUNT          252 a          836 c          780 g          336 t
ORIGIN      
        1 cgattcgcgg tcgtcgtccg tgatcacgcc gacgacggcc gccttggcgc gcgtgcgtgc
       61 gagggcgtcg acccatccgt cgaccgtgac gtccgagccg aggtacagca caccgatgcc
      121 gcgccgccgc agcgccgcgg cgaaggcgag cgcgccgagc tcgtggcgcg accccggtgg
      181 caggccgacg acgacggaca ccttgcgcgg tgctccggcg gcctggaacg cggccgccag
      241 ccggcgaccc atcgccccgc tcgcggcgtg ctcgccggcc acgctcaggc gtccctcggc
      301 ccaggcgtcg ccgagcgccg ccgcggccgg caggagcagg tcgtcgacga tggactcgaa
      361 cgatcccgag gcgagcatct cgtccaggac cgactccgtg tcgggcgccg agttcgcctc
      421 ggccgcgcgc acaaaccgcg cgatcagctg ctggcgatgg tccggcccag gcgatggtcg
      481 gccagaggcc agggtcggtc gcgcgacggg gacgacgtcg tcgagacgga cctcgcccga
      541 cccgatcgcg cgggcagcct cggaggccgt ccagccagat gcaacgagcg attgcatggc
      601 gaccagcacc cggatcgtcc cgtcgtcgta gaggcgatat ccgctcggtg tccggactgg
      661 ccggatcacc ccgtaccgcc gctcccacgc gcggatgagg ggcgccccga cgccggatcg
      721 cgccgacgcc tccttgatgg tgtacatggt ctgtcgaatg ttagcagggg ccgtcgcgac
      781 ccccgggcat cggtggcacg atgggcccgt gaccgacccg ccgcccacgc cccgccggcg
      841 cttcggcggc ctgctcgtcg acccggcccc gctgcggctc gatcgggact tccggctgct
      901 ctggatcggc cagggcatca gcaccgtcgg gcgcacgttc accctggtgg cggtgccgta
      961 ccaggtctac gtgctcaccg gcgacatcct cgcggtcggc gcgctgtcgg tcgtccagct
     1021 cgccgcgacc ctcatcttcg ccctcggcgg cggcgcggtg gcggacgcgg tcgaccggcg
     1081 ccgcctgctc ctgttcaccc aggtcggcca agccggctgc agcgccgcct tcctgctcat
     1141 cgccctgtcc ccgtcgccgt cgctcctcgc gatcttcgcc gtcatgttcg tgtccatggg
     1201 gctgggcgcg atcgatggcc cggcgcgcgc gtcggccgtt ccccgcctcg tgccgatcga
     1261 gcggctgcag gccgccatcg ggctcaacca gctgatgttc aacgcggcct gggtcatcgg
     1321 gccggcgctg ggcgggatcg tgctcgcgac ggcggggccg gcggccgcct acgccatcga
     1381 catcgtcacg ttcgccgcgg tgatcgtcgc gctgctgctc atctccccga tcccgccggc
     1441 gcccgacgcc gtccgcccca gcatcggtgc cgtcgtcgag ggcctgcgct ttgcgcgccg
     1501 gcggcgcgag gtgctggcga cgttcatcgt cgacctcaac gcgatgatct tcgggtcgcc
     1561 agcatcgctg tatccggcgc tcgcgctcga cgtgttcatg gtcgggcccg ccggcgtcgg
     1621 gctgatgtgg tcggcgacgg ggttcggggc gatggtcggc gccctgttca gcggctggac
     1681 gaccaccgtc cggcgaacgg gccgcgccgt cctcatcgcg gtcgccgtgt gggggctggg
     1741 gatcctcggc ttcgggctcg cgacgttcag cttccccttg gcgctgctgt tcctcgccat
     1801 cgccgccggg gcggacgtga tcagcgccgt cctgcgcagc tcgatcgtcc agatcctgac
     1861 gcccgacacg ctgcgtggcc gggtgtcatc gatccacatc ctcgtggtga cggccggccc
     1921 ggagctcggc aacgcggagg ccagcgtggt cgcctcggtc atcggcaccc agggcgccgt
     1981 cgtctccggc ggcctgctga cgctggccgg gctgggcgcc atcgccctcg cgatgccgga
     2041 gttccgacgg ctcgacatgc ccaccgccat cgccgacacc gccgcggcgg cacacgcggt
     2101 cggctgatcg cgccccgccc ggggtgacaa tgggcggacc accatgagga gtcacggttg
     2161 gcggtcagcg ccttcgacct gttcaagatc ggcatcgggc cctc
//
LOCUS       DBNHXO010000449         6256 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3909638, whole genome shotgun sequence.
ACCESSION   DBNHXO010000449 DBNHXO010000000
VERSION     DBNHXO010000449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3909638"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..854
                     /locus_tag="AC162A_09035"
     CDS             174..854
                     /locus_tag="AC162A_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR5011026.1"
                     /translation="MITLSVLRVFSDADGRGGNPLGVVLDGGSVPADRRQAVAAELGY
                     SETVFVDDADDGSIRIFTPASELRFAGHPTVGTGWLLRSLGHAGSVLRCPAGDVVTWQ
                     DSDLSWIRARPAWVHPITRAQLASAADVEALAGPPPGEGSYYAWAWIDESEGTLRSRY
                     FVLELGIGEDEATGAAAVMMGGLLGRPLEIHQGVGSVLHVRSGPDGTIEVGGRVDVVG
                     SRELAVQA"
     gene            851..1213
                     /locus_tag="AC162A_09040"
     CDS             851..1213
                     /locus_tag="AC162A_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011027.1"
                     /translation="MSETVDPVVRTASPSPAAFHYGPAHLIMHGNAAFLAAFGRGSLG
                     MPAREVMTELPRSAFELMDRVYREGRPLARRLVLPGGEHRLVVVPRADPESGDVYGVT
                     THLRAQGPPVHPEEETNP"
     gene            1210..1515
                     /locus_tag="AC162A_09045"
     CDS             1210..1515
                     /locus_tag="AC162A_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR5011028.1"
                     /translation="MSAVVVNHLQLAIPADDFAATVQREFPPALDAQPGFERFYLVKT
                     AEDAVTVVIVWSSQEAAATGSAVIGPTLFHDYVVPLLRAPQNRVVGDAVVVHEAGRS"
     gene            1593..2813
                     /locus_tag="AC162A_09050"
     CDS             1593..2813
                     /locus_tag="AC162A_09050"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR5011029.1"
                     /translation="MSTELDAPNRMDRGAFEASLPRAAYVDDAFLDREREGIWWSEWV
                     VAGRVEQVPEPGDFLAVDVAGEQVIVVRNRSGEISAHYDVCRHRGSRLTGPDQREDPA
                     SGDAAGASGRFKGVIRCPYHSWCYELDGRVRNAPFLGESDAFEPADFGLYPVAVEAWG
                     GWLFVNLSPERVSAGASLAAQLGGIPDRVARYPLADLRAMRRIVYDVRANWKVIVENY
                     NECYHCSGVHPELCRIVPAFKEKGSSNLDWDAGVPQAEGTFTFTMTGRSDRAPFPGLD
                     EDEQVRHKGELAYPNLMLSLSADHAAAFTLIPKAPDRTLVVVDFLFHPDEIAKPTFDP
                     SDAVDFWDLVNRQDWAVCEGVQSGMTSRRFRFGWYAPMEDLSLDIRRYIGDRRGAAAV
                     EVDVTPGGGHGGRA"
     gene            2810..4000
                     /gene="solA"
                     /locus_tag="AC162A_09055"
     CDS             2810..4000
                     /gene="solA"
                     /locus_tag="AC162A_09055"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HIR5011030.1"
                     /translation="MTRDWDVVVVGLGALGSAAAYWCSRRTGLRVLALERYEIGHTNG
                     ASEDVSRIIRRSYHRRDYVRLTARAYESWAEVERESASQVVFRTGGLDVGPRQTGEGV
                     AIDIGEYSQAMIAEGVPFETLDGPEVMRRWPAWRLDDGHVGLFQADAGLADPSRGNLA
                     HRRLATAHGAVLRERAPVARIEDAGGETTVELEDGERLTASRVIVATDAWTNDLLEPL
                     GASLPLTVTQEQVSWFTPGVDPSLFDPARFPIWIWMDEPSFYGFPTHGHPGPKIGQDV
                     GGQRVTPATRTFEPDQAALGRVRQFLDARLPGMAGEPFLIKTCLYTLTPDRDFVLDAV
                     PGHANVLVALGSAHGYKFASVIGRIMAELALEGVTPSAGEIEAFGFGRAALSGAAEPA
                     TFVI"
     gene            4157..6100
                     /locus_tag="AC162A_09060"
     CDS             4157..6100
                     /locus_tag="AC162A_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5011031.1"
                     /translation="MNPLPRRRMARLTAAFALAFSILLPAAVPAAAAEPGVLQVGTTQ
                     DLDATNPFNTELVTGYEAFQLTYNLLTEYDKDANPAAGFSDTWERADDRVTFHIRDGM
                     QWSDGTPATSKDVCFSWGLALAANKAKGVIGSGYLDAGLKDAGVTRIECPDDNTFIAY
                     TTDASDRIFQIYVPILPEHIWGKFNYKNIDKEKFDGPLVGTGPYLLQEWKTGQFARFA
                     RNPTFWGEQGVADEVILRFFADSTDTMVQALKAGELDYVHNANADQFKQLQADPTYTA
                     VAGKANGWTQLAFNTYGTGTGKEIKGGGPSTKALLDPAFRDALGYAVDKEALVERVLG
                     GFGDVGTTIVPPILADWHVEPDNPRHFDIELAKQKLDAAGYLLDPATGKRLDKEQKPI
                     SLRLAYPNTNDTYAKSAQFVKEWYALLGVDVTTQSYDSDTLTELLYPPEGGDPSYKAN
                     YDIELWGWSGSPDPNGLTIVMRCDQIGNLSDSQYCNPEYDALYDQQSKEGGEARHATL
                     AKMQNLIYDEAPYDVLYYDANLDVYRNDRFAGWQNMPAEGTPFFTYGILDYTLLRDVN
                     EEPPATPEAPTAGASTEPGASAPAAQTSAPTATAGTGSGDSASGSGSNTTLLLGVVAI
                     VVVVVAGGLLWSRRRAAPGVEDE"
BASE COUNT          929 a         2229 c         2207 g          891 t
ORIGIN      
        1 gtgatcttcg tcgcgaccgg cctccgcccc ggcgtcgcga caccggacgc gaccgagcag
       61 ctcgccgtgc gctgggcgag cctggacgag gtgctgggcg agatcgcagc gggcgagatc
      121 cacgacctga tcacgatcgc cggcgtcggc gcctacgccg cgggcggcgc ccgatgatca
      181 ccttgagcgt cctgcgcgtg ttcagcgatg cagacggccg gggcggcaat ccccttggcg
      241 tggtcctcga cggcggctcg gtgccggccg atcgccgcca ggccgtcgcc gccgagctgg
      301 gctactcgga gaccgtgttc gtcgacgacg ccgacgacgg gtcgatccgg atcttcactc
      361 cggcgtccga gctgcgcttc gcgggccacc cgaccgtcgg caccgggtgg ctgctgcgct
      421 cgctcgggca cgcggggtcc gtcctgcgct gtccggcggg cgacgtcgtc acgtggcagg
      481 acagtgacct gtcgtggatc cgtgcccgac cggcctgggt gcacccgatc acgcgggccc
      541 agctcgcatc ggcagcagac gtcgaggcgc tggccggacc gccgccgggc gagggctcgt
      601 actacgcctg ggcgtggatc gacgagtccg agggcaccct ccgatcccgc tacttcgtgc
      661 tcgaactggg gatcggcgag gatgaggcca ccggggcggc cgccgtgatg atgggcgggc
      721 tgctcggccg gccgctggag atccaccagg gcgtcgggtc ggtgctgcac gtgcgatcgg
      781 gacccgacgg gaccatcgag gtcggcgggc gggtcgacgt cgtcggctcg cgggagctgg
      841 cggtccaggc gtgagcgaga ccgtcgaccc cgtcgtgcgc accgccagcc ccagcccggc
      901 ggccttccac tacggccccg cgcacctgat catgcacggc aacgcggcgt tcctcgccgc
      961 ctttggccgt gggtcgctgg gtatgcccgc ccgcgaggtc atgaccgagc tgccccgttc
     1021 cgcgttcgag ctcatggatc gggtttaccg tgaaggtcgg ccactggctc gccggctggt
     1081 gctgccgggt ggcgagcatc gcctggttgt cgtcccgcgc gcggaccccg agtcgggcga
     1141 cgtctacggc gtcacgaccc acctgcgggc ccaggggccg ccggtccacc ccgaagagga
     1201 gacgaaccca tgagcgcggt ggtggtcaac cacctccagc tggcgatccc ggccgacgac
     1261 ttcgctgcga cggtgcagcg ggaattcccg ccggcgctcg acgcgcagcc gggcttcgag
     1321 cggttctatc tggtcaagac ggccgaggac gccgtgacgg tcgtgatcgt ctggtcgagc
     1381 caggaggccg ccgcgacggg gtcggcggtg atcggcccga ccctgttcca cgactacgtc
     1441 gtcccgctgc tccgggcccc gcagaaccgc gtcgtcggcg acgcggtcgt cgtgcacgag
     1501 gcaggacgca gttgaggcgc gcggcgccga ccggctagca tcggccggcc ggcctcgtgg
     1561 cgccggcacc gcgactcccg aggattgagc cgttgtcgac cgagctcgac gcccccaacc
     1621 gcatggatcg tggcgccttc gaggcgtcgc tgccccgggc cgcctacgtc gacgacgcgt
     1681 tcctcgaccg cgagcgcgag gggatctggt ggtcggagtg ggtcgtggcg ggccgcgtgg
     1741 agcaggtgcc tgagcccggg gacttcctgg cggtcgacgt cgccggcgag caggtcatcg
     1801 tcgtacgcaa ccggagcggc gagatcagcg cccattacga cgtctgccgc caccgcggct
     1861 cgcggctgac cggcccggac cagcgggaag acccggccag cggcgacgcc gccggcgcgt
     1921 ccgggcggtt caagggcgtc atccgctgcc cgtaccactc gtggtgctac gagctcgacg
     1981 ggcgcgtgcg caacgcaccg ttcctcggcg agtcggacgc cttcgagccc gccgacttcg
     2041 gcctgtaccc ggtcgccgtc gaggcatggg gcggctggct gttcgtcaac ctgtcccccg
     2101 agcgcgtctc cgctggcgcg agcctcgccg cccagctggg cggaatcccg gaccgggtcg
     2161 cgcgctaccc gctggccgac ctgcgggcca tgcgccggat cgtgtacgac gtgcgggcca
     2221 actggaaggt catcgtcgag aactacaacg agtgctacca ctgctcgggc gtccaccccg
     2281 agctgtgccg gatcgtgccg gcgttcaagg agaagggcag cagcaacctc gactgggacg
     2341 ccggcgtgcc ccaggccgag gggacgttca cgttcacgat gaccggccga tccgaccgtg
     2401 cgccattccc ggggctcgac gaggacgagc aggtgcgcca taagggcgag ctcgcctacc
     2461 cgaacctcat gctcagcctg tcggccgatc atgcggcggc gttcacgctg attcccaagg
     2521 ccccggaccg gaccctcgtc gtcgtggact tcctgttcca ccccgacgag atcgcgaagc
     2581 ccaccttcga cccgtccgac gccgtcgact tctgggacct cgtaaaccgc caggactggg
     2641 cggtgtgcga gggcgtgcag tcggggatga cctcgcggcg gttccggttc ggctggtatg
     2701 caccgatgga ggacctgagc ctggacatcc ggcgttacat cggcgatcgg cggggggcgg
     2761 cggccgtcga ggtggacgtc acgcccggcg gcggccatgg cgggcgggca tgacccgcga
     2821 ctgggacgtc gtcgtcgtcg ggctcggcgc gctcggctcc gcggctgcct actggtgctc
     2881 gcggcggacg gggctgcggg tcctcgccct cgagcggtac gagatcgggc acaccaacgg
     2941 cgcgtcggag gacgtgagcc ggatcatccg ccgctcctac caccggcggg actacgtcag
     3001 gctcaccgcg cgggcctacg agagctgggc cgaggtcgag cgcgagagcg cgtcgcaggt
     3061 ggtgttccgg accggtggcc tggacgtcgg gccgcgccag accggcgagg gcgtggcgat
     3121 cgacatcggc gagtactcgc aggcgatgat cgccgagggc gtgcccttcg agacgctcga
     3181 cgggcccgag gtcatgcgcc gctggccggc ctggcgcctc gacgacgggc acgtcgggct
     3241 gttccaggcc gacgccgggc tcgccgaccc gtcgcgcggc aacctcgccc accggcggct
     3301 ggcgacggcc catggcgccg tgctgcggga gcgggccccg gtcgcgcgaa tcgaggatgc
     3361 cggcggcgag acgaccgtgg agctcgagga cggcgagcgc ctgacggcca gcagggtcat
     3421 cgtggccacc gacgcctgga ccaacgacct gctcgagccg ctcggggcga gcctgccgct
     3481 caccgtgacc caggaacagg tcagctggtt cacgcctggc gtcgatccgt cgctgttcga
     3541 cccggcccgc ttcccgatct ggatctggat ggacgagccc tcgttctacg gcttcccgac
     3601 ccacggccac ccgggcccca agatcggcca ggacgtcggc gggcaacggg tcacgccggc
     3661 cacgcggacc ttcgagcccg accaggcggc gctgggccgg gtccggcagt tcctcgacgc
     3721 gcgcctgccc ggcatggccg gcgagccgtt tctcatcaag acctgcctgt acacgctgac
     3781 cccggaccgg gacttcgtcc tggacgcggt gccggggcac gcgaacgtgc ttgtcgccct
     3841 gggctcggcg cacggctaca agttcgcgtc ggtgatcggc cggatcatgg ccgagctggc
     3901 gctcgaaggg gtgaccccgt cggcgggcga gatcgaggcc ttcggcttcg ggcgggcagc
     3961 gctgtcgggc gcggccgagc ccgcgacctt cgtcatctga tggcccgtgt cgcgcgacgt
     4021 gggcccccgg aacaggtagg ttgcaggagt cgaggtcgtg tgccacgctc ccgcgcaccc
     4081 gccgagcaat cggcgcgacc gtcagaaccg tggaggagcg gttcgtcggt cagcttccca
     4141 ggaggaaccc cccgcaatga acccgcttcc ccgtcgccgg atggcgcggc tgacggccgc
     4201 gtttgcgctc gccttctcga tcctgctgcc ggcggcggtt cccgcggccg cggccgagcc
     4261 aggcgtgctg caggtcggca ccacccagga tctcgacgcg accaacccgt tcaacacgga
     4321 gctcgtcacc ggctacgagg cgttccagct gacctacaac ctgctgaccg agtacgacaa
     4381 ggacgccaat ccggcggccg gcttctcgga cacgtgggag cgggccgacg atcgcgtcac
     4441 gttccacatc cgggacggga tgcagtggtc ggacgggaca ccggcgacgt ccaaggacgt
     4501 ctgcttctcg tggggcctcg cgctggccgc gaacaaggcc aagggcgtca tcggatccgg
     4561 ctacctcgat gcgggcctca aggacgccgg cgtgaccagg atcgagtgcc ctgacgacaa
     4621 cacgttcatc gcctacacga cggacgcgtc agaccgcatc ttccagatct atgtgccgat
     4681 cctgccggag cacatctggg gcaagttcaa ctacaagaac attgacaagg agaagttcga
     4741 cggcccgctc gtcggcaccg gaccctacct cctccaggag tggaagacgg gccagttcgc
     4801 gcggttcgcc cgcaacccca ccttctgggg cgagcagggc gtcgcggacg aggtcatcct
     4861 ccggttcttc gccgatagca ccgacacgat ggtccaggcg ctcaaggccg gcgagctcga
     4921 ctacgtgcac aacgcgaacg ccgaccagtt caagcaactc caggccgacc cgacctatac
     4981 cgccgtcgcc ggcaaggcca acggctggac ccagctcgcc ttcaacacct acggcacggg
     5041 cactggcaag gagatcaagg gtggcgggcc gtctaccaag gccctgctcg accccgcgtt
     5101 ccgcgatgcg ctcgggtacg ccgtggacaa ggaggcgctc gtcgagcgcg tcctgggcgg
     5161 cttcggcgat gtcggtacga ccatcgtccc gccgatcctc gcggactggc acgtcgagcc
     5221 cgacaacccc cgtcacttcg acatcgagct ggcgaagcag aagctcgacg cggccggcta
     5281 cctgctcgac cccgctaccg gaaagcgcct ggacaaggag cagaagccga tcagcctgcg
     5341 gctggcctat cccaacacca atgacaccta cgccaagtcg gcccagttcg tgaaggagtg
     5401 gtacgcgctg ctcggggtcg acgtcaccac gcagagctac gacagcgaca ccctgaccga
     5461 gctgctgtat ccgcccgaag ggggcgaccc ctcctacaag gccaactacg acatcgagct
     5521 ctggggctgg tccggcagcc cggacccgaa cgggctgacc atcgtcatgc gctgcgacca
     5581 gatcggcaac ctgtccgaca gccagtactg caaccccgag tacgacgcgc tgtacgacca
     5641 gcagtcgaag gaggggggcg aggcgcgcca cgcgacgctg gccaagatgc agaacctgat
     5701 ctacgacgag gcgccgtacg acgtcctgta ctacgacgcc aacctcgacg tgtaccgcaa
     5761 cgaccgcttc gccgggtggc agaacatgcc cgccgagggc accccgttct tcacctacgg
     5821 catcctcgac tacacgctcc tccgtgacgt gaacgaggag cctcccgcga ccccggaggc
     5881 gccgaccgcc ggcgcatcga cggagcccgg ggcgagcgcc ccggccgctc agaccagcgc
     5941 gcccaccgca acggccggca cgggatcggg cgattcggcg agcggctccg ggtccaacac
     6001 gacgctcctg ctcggggtcg tcgcgatcgt ggtcgtggtc gtcgccggcg gcctgctgtg
     6061 gtcgcggcgt cgcgccgcgc ccggcgtcga ggacgagtag ccggccgacg tgtgacgccg
     6121 aacgcatcgg ccgcaccggg aggaggtgag gggtgagcgg caggtacatc ctgcgcaagc
     6181 tcctcgcctc gctggtgacg atcgtggcga tcgtcatcct caacttcgtg ctgttccgga
     6241 tgatgccggg ctcccc
//
LOCUS       DBNHXO010000450         2276 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_932513, whole genome shotgun sequence.
ACCESSION   DBNHXO010000450 DBNHXO010000000
VERSION     DBNHXO010000450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_932513"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="AC162A_09065"
     CDS             complement(<1..325)
                     /locus_tag="AC162A_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011032.1"
                     /translation="MTGATDDASGVEPWAPDPASSPDPDPGAEPAGFIVVAAGDVRLH
                     FHDWGGPRADAGRARPGVLLLPGLLQPAWSWAPVARRLRGLAPTVVADLRGQGLSDAP
                     MDGYDP"
     gene            347..766
                     /locus_tag="AC162A_09070"
     CDS             347..766
                     /locus_tag="AC162A_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HIR5011033.1"
                     /translation="MAKRRRGSRFAAHRPRASRHEPFERLVDRAVAGIPQPFRAALDE
                     VAIVIADDPTAEQLRENGLRPDETLYGLYEGVPRSEWGADWAPIPTRILVFRLPLEED
                     FPDPDDLADEVRITVLHELAHHLGIDDEDHLAALGID"
     gene            complement(778..1923)
                     /locus_tag="AC162A_09075"
     CDS             complement(778..1923)
                     /locus_tag="AC162A_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011034.1"
                     /translation="MGPERDHRQPPPPPDPAITRLLYRGPYAVAPQPPPSPAALFIAA
                     QPPVRDEVKAIPTGIRALVGASLDLLFRRDAGLRSPSFYVGFMLLVTLGPLAAMLGMQ
                     VALGNALLEVDVAPTPLDPWIALAALPAILGYLVASVESRTLATAVTAARMEGRPIAL
                     PESVGIARRRFWRMFAAQFVVTFLTLVIATVLTVVVDVALLEVGPIGLIDVGISLVVG
                     VAVAWPFVYVPAGLVLGEVGVAEAISRSIRLARLRGRLALVLALFASAPQILVGVGIG
                     TGLDIVIRLAGGPDALDTFPLPLVVPAAAILSFAFGTLVFLSEAVAASPAVHAFASLT
                     HYTHGLEIGREKPVDVRRPWAPWMTPGLAAGAVIAVLSLVGGIQAIG"
     gene            complement(1938..>2276)
                     /locus_tag="AC162A_09080"
     CDS             complement(1938..>2276)
                     /locus_tag="AC162A_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR5011035.1"
                     /translation="EAGEPVRVLPASVADGLGAPSAGRHALRAVQRYVEEVVLIDDPT
                     ILAGLRFGIERLKQVLEPAGSAALAAVLTGAVPLRPGDRVCVILSGGNVAVERLGELL
                     AAAAPLQAAG"
BASE COUNT          362 a          847 c          846 g          221 t
ORIGIN      
        1 cggggtcgta gccgtccatc ggcgcgtccg acagcccctg gccacgcagg tcggcgacga
       61 ccgtgggggc aagcccgcgg agacggcgcg cgacgggcgc ccacgaccag gcgggctgga
      121 gcaggccggg caggagcagg acgccggggc gggctcgacc ggcgtccgcg cgcgggccgc
      181 cccagtcgtg gaagtggagc cgaacgtcgc cggcggcgac gacgatgaac ccggcgggct
      241 cggcgccggg atcgggatcc gggctggagg cagggtcggg cgcccacggc tcgacgccgg
      301 aggcgtcatc ggtggcgccg gtcatcgcgg ctaggatacg ggcgcgatgg ccaaacgcag
      361 acgcggctcc cggttcgcgg cccatcggcc gcgcgcatca cgccatgagc cgttcgagcg
      421 actcgtcgac cgcgccgtcg ccggcatccc gcagcccttt cgggcagcgc tcgacgaagt
      481 cgctatcgtc atcgccgacg atccgaccgc cgagcagctg cgcgagaacg gcctgcggcc
      541 ggacgagacc ctgtacggcc tgtacgaggg ggtgccacgc tccgagtggg gcgcggactg
      601 ggcgccgatc ccgacccgga tcctcgtgtt ccgcctgccg ctcgaggagg acttccccga
      661 cccggacgac ctcgccgacg aggtgcggat cacggtcctc cacgagctcg cccaccacct
      721 cggcatcgac gatgaggacc acctcgccgc gctcggcatc gactaactga acgacaacta
      781 gccgatcgcc tggatcccgc cgacgaggga gagcacggcg atgacggccc cggccgcgag
      841 tcccggggtc atccagggcg cccacggccg gcggacgtcg accggcttct cccgcccgat
      901 ctcgaggccg tgcgtgtagt gggtcagcga cgcgaacgcg tgcacggccg gcgacgcggc
      961 gacggcctcg gacaggaaca cgagcgtgcc gaacgcgaac gacaggatcg cggcggcggg
     1021 gaccacgagc ggcagcggga atgtgtccag cgcgtccgga ccgccggcga ggcggatcac
     1081 gatgtcgagg cccgtcccga tcccgacccc gaccaggatc tggggtgccg acgcgaacag
     1141 cgcgagcacg agcgccagcc gcccccgcag ccgcgccagc cggatcgagc gcgagatggc
     1201 ctcggccacg ccgacctcgc ccagcacgag gccggcgggc acgtacacga acggccaggc
     1261 gacggccacc ccgacgacca ggctgatccc gacgtcgatc aggccgatcg gaccgacctc
     1321 gagcagcgcc acgtcaacga cgacggtcag gacggtcgcg atgaccagcg tcaggaacgt
     1381 caccacgaac tgtgcggcga acatgcgcca gaaccgccgg cgcgcgatcc cgaccgactc
     1441 gggcagggcg atcgggcggc cctccatccg ggccgcggtc acggccgtgg caagcgtccg
     1501 cgactcgacg ctcgcgacga ggtaacccaa gatcgccggc agcgccgcca gcgcgatcca
     1561 cggatcgagc ggcgtcggcg ccacgtcgac ctcgagcagc gcgttaccaa gcgcgacctg
     1621 catcccgagc atcgccgcca gcggcccgag cgtcaccagc agcatgaagc cgacgtagaa
     1681 cgagggcgag cgaaggcccg cgtcgcggcg gaacaggaga tccagcgatg ccccgaccag
     1741 cgcccggatc ccggtcggga tcgccttcac ctcgtcgcgc accggcggct gggcggcgat
     1801 gaacagcgcc gccggcgacg gcggcggctg cggggcgacc gcgtagggcc cgcgatacaa
     1861 gaggcgcgtg atggcgggat cgggcggcgg cggcggttgg cggtggtcgc gctcggggcc
     1921 cacgccggcg gcggccgtca gcccgcggct tggagcggtg cggccgcggc gagcagctcg
     1981 cccaggcgct cgaccgccac gttgccaccg gagaggatga cgcacacccg gtcgcccggt
     2041 cgcagcggca cggcgcccgt gaggaccgcc gccagcgccg cggacccggc aggctcgagg
     2101 acctgcttga gccgctcgat gccgaaccgc agcccggcga ggatcgtcgg atcgtcgatc
     2161 aggaccacct cctcgacgta gcgctggacg gcgcgcagcg cgtggcggcc cgcggacgga
     2221 gcacccagcc cgtcggcgac gctggccggg agcacccgga ccggctcgcc cgcctc
//
LOCUS       DBNHXO010000451         2530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_932658, whole genome shotgun sequence.
ACCESSION   DBNHXO010000451 DBNHXO010000000
VERSION     DBNHXO010000451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_932658"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="AC162A_09085"
     CDS             <1..213
                     /locus_tag="AC162A_09085"
                     /inference="COORDINATES: protein motif:HMM:NF019887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HIR5011036.1"
                     /translation="MTAGDALNDLEMIEGAGHGAAMASAPEVVRAAARYVAAPVDDDG
                     VAELSEALVLAPPEEAARNGARMAVS"
     gene            210..860
                     /locus_tag="AC162A_09090"
     CDS             210..860
                     /locus_tag="AC162A_09090"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR5011037.1"
                     /translation="MTGTRVLPDGEATRADAVEHLRAGRIVAIPTDTVYGIAADLALP
                     DAIERLFAAKQRPPEKAVAVLLADAEQAWTLGLASPAARVLADAFWPGGLTLVLTVQP
                     EARLPTALAAGAPTIGVRVPDHPAPRALAAVLGPLPTTSANLSGAADARDAAEVAATL
                     GDAVALVIDGGPIRGGPASTVVDCTSERPAILRSGAIDPALIARALEEAGIPHAIG"
     gene            1440..>2530
                     /locus_tag="AC162A_09095"
     CDS             1440..>2530
                     /locus_tag="AC162A_09095"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR5011038.1"
                     /translation="MELERHRQADKIELIASENYVYAAVLEAQGSWLTNKYAEGLPGK
                     RYYGGCEYVDIAENLARDRALALFPGSDHVNVQPHSGAQANMAAYFAVLRPGDRILGM
                     NLAHGGHLTHGSPVNFSGRLYEVHAYGVTRGDERIDYDALEAQAAEVRPKLVVAGASA
                     YPRTWDFERMADIAHAVGALLFVVMAHVAGLVAAGLHPSPFPHADLVTTTTHKTLRGP
                     RGGVVFAREDLPNSIDRADFPALGKNTLAQAIDKSVFPGVQGGPLMHVVAGKAVAFKL
                     AAGGDFRRDQARTIENARVLAQTLADAGARLVSGGTDNHLALVDVTPLGVTGKEAEAL
                     LDDVGITVNKNAIPFDANPPNIASGIRVGT"
BASE COUNT          363 a          936 c          894 g          337 t
ORIGIN      
        1 atgaccgcgg gtgacgcgct caacgacctc gagatgatcg agggcgccgg ccacggggcg
       61 gcgatggcct cggcgccgga ggtcgtgcga gcggccgccc ggtacgtggc ggcgccggtc
      121 gacgacgacg gggtcgcgga gctgagcgag gcgctggtgc tggcaccgcc cgaggaggcg
      181 gcccgcaacg gcgcgcggat ggcggtctcg tgacggggac ccgggtgctc cccgacggcg
      241 aagcgacgcg cgcggacgcg gtcgagcacc tgcgtgcggg ccggatcgtc gcgatcccga
      301 ccgacacggt ctacgggatc gccgcggacc tcgcgctccc cgatgccatc gagcggctgt
      361 tcgccgccaa gcagcgtcca cccgagaagg cggtcgccgt cctgctggcc gatgccgagc
      421 aggcgtggac cctggggctg gcgtcccccg cggcgcgcgt cctcgccgac gcgttctggc
      481 ccggcggact gaccctcgtc ctgaccgtgc aacccgaggc ccgcctgccg accgcgctcg
      541 ccgccggcgc gcccaccatc ggcgtccgcg tgcccgacca cccggcgccg cgtgcgctgg
      601 ccgccgtgct cggacccctg ccaacgacct cggccaacct ctcgggcgcg gccgacgcgc
      661 gcgacgcggc cgaggtcgcc gcgacgctcg gcgatgcggt cgcgctggtc atcgacggcg
      721 gcccgatccg cggtggcccg gcctcgacgg tcgtcgactg cacgagcgaa cgccccgcga
      781 tcctgcgatc gggggcgatc gatccggcgc tgatcgcgag ggccctggag gaggccggga
      841 tcccgcacgc gatcgggtga tgggaccctg gtcacgggac ttcgtgtcct tggccggtcg
      901 ccaccccgga tccacaggcc tctcgatcac caggtacgac ggtccttcgt gcacccgcca
      961 ggcgtcggcg ctgttggcgt tcatccacga gcgtggctcg tcgtatcgac ccgcacgcca
     1021 ccatctcgac ccacggacga cgaggcatcg tgcttggcgc aattggcgcg gcatgttcgc
     1081 gccatccacg aaatgtcatt cgtaagcgac gcctgcgaag ccggacggcg agaggccgtc
     1141 agggacggtc gcagacgagg gcgccgccgc cgtgacggga aggtcatctg tacgacccat
     1201 gttcgtaccc ggcggcggcg gattggcgcg acctcgagcg ggcggaacat gccgcctgtc
     1261 ggggcggccc gtgggacaat gggcccgccc cgatgcttgc gcccgattcg cctggaggtc
     1321 tgcccgtgtc cgaagccacc gtcgcgccgg cagcctcccc ggagaccccg tcggcgaccg
     1381 ccgtcccgga gtcggcctgg gcgtcaatcg ccgagctgga tcccgagctg tgggacgcga
     1441 tggagctgga gcgccaccgc caggccgaca agatcgagct gatcgcgagc gagaactacg
     1501 tgtacgcggc ggtgctcgag gcacagggtt cgtggctcac caacaagtac gccgagggtc
     1561 tgccgggtaa gcgctactac ggcggctgcg agtacgtgga catcgcggag aacctcgccc
     1621 gcgaccgcgc gctggccctg ttccccggct cggaccacgt caacgtccag ccccattcgg
     1681 gcgcccaagc gaacatggcc gcctacttcg cggtcctccg gccgggcgac cggatcctgg
     1741 gcatgaacct cgcccacggc ggtcacctga cccacggctc gcccgtgaac ttctcgggcc
     1801 gcctgtacga ggtgcatgcg tacggcgtca cccggggtga cgagcggatc gactacgacg
     1861 cgctcgaggc gcaggcggcc gaggtgcggc cgaagctcgt ggtcgccggc gcgtccgcct
     1921 atccgcggac ctgggacttc gagcggatgg ccgacatcgc tcacgccgtc ggagcactgc
     1981 tgttcgtggt catggcccac gtcgcaggcc tcgtcgccgc gggcctccat cccagcccgt
     2041 tcccgcacgc cgacctcgtc accacgacca cccacaagac ccttcgtgga ccacgcggcg
     2101 gggtcgtgtt cgcgcgtgag gaccttccga acagcatcga ccgggccgac ttcccggcgc
     2161 tgggcaagaa caccctcgcg caggcgatcg acaagtccgt gttccccggc gtccagggcg
     2221 ggcccctcat gcacgtcgtc gccgggaagg ccgtggcgtt caagctggcg gccggcggcg
     2281 atttccgtcg cgaccaggcc cgaaccatcg agaacgcccg agtcctcgcc cagacccttg
     2341 ccgacgcggg agcgaggctc gtcagcggcg gcacggacaa ccacctggcc ctggtcgacg
     2401 tgacgccgct gggtgtcacc gggaaggagg ccgaggccct cctcgacgac gtggggatca
     2461 ccgtcaacaa gaacgcgatc ccgttcgacg cgaacccgcc caacatcgcg tccgggatcc
     2521 gcgtcgggac
//
LOCUS       DBNHXO010000452         2047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4505868, whole genome shotgun sequence.
ACCESSION   DBNHXO010000452 DBNHXO010000000
VERSION     DBNHXO010000452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4505868"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="AC162A_09100"
     CDS             <1..1050
                     /locus_tag="AC162A_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012408925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5011039.1"
                     /translation="RLFRSFSQVDVSISRRFGGTGLGLAISRRLAELMDGSVTAESSG
                     VPGEGSTFRLVIRADEAALPVPAPAADPIDIAGRTVLVVDDNATNLRILDAQLGRWRM
                     AVRSTRSPAEALAWVQAGASFDLAIVDFHMPVMDGLTLGHELQAVHPEAPLPIVIVSS
                     VGARDRRESFVAAELTKPVKPSALHDAVMTALGRVTRPEAVPVAKASPDARLAEDRPH
                     RILLAEDNAVNQKLALRLLERMGYTADVAESGQEVLDALERDPYDLVLMDVQMPEMDG
                     LEATRRARARWRERSLWIIAMTANAMEGDREMCLAAGMNDYISKPIRPDVLAGALASA
                     PVAAETSVARGQARG"
     gene            1043..1405
                     /locus_tag="AC162A_09105"
     CDS             1043..1405
                     /locus_tag="AC162A_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013767.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR5011040.1"
                     /translation="MAETLDAAVLAELLEAVGDDPAFVDELVDTFLAEAPGFMSAIDG
                     AVAAGDAGALLVPAHTLKGNASTIGAGRLAEISRTLEERARRGDLDGAGSDAAAARAE
                     LAHVVTALDAQRARRWTA"
     gene            1402..>2047
                     /locus_tag="AC162A_09110"
     CDS             1402..>2047
                     /locus_tag="AC162A_09110"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5011041.1"
                     /translation="MSEGGRILVVDDSRLNRQTLARLLGGLGHEVVEAEDGRVALELL
                     GEDGAGIDVVLLDLVMPELDGFSVLAAIRANPALARIPVIVVSGLDDLDGIVRCVEMG
                     AADYLPRPIKPTLLRARVATSLAEKRLRDDNARLLATVDRQREELSRFLSPQVAALVS
                     TPEGEQLLAGHRRVVTAVFCDLRGYTAFGEIAEPEELLDVVREYHAAMGRRIIEF"
BASE COUNT          274 a          734 c          757 g          282 t
ORIGIN      
        1 cgcctgttcc ggtcgttcag ccaggtcgac gtgtcgatct cccgccggtt cggcgggacc
       61 ggcctggggc tcgcgatcag ccggcggctg gccgagctga tggacggcag cgtgaccgcc
      121 gagagcagcg gggtcccggg cgagggcagc acgttccgcc tggtcatccg ggccgacgag
      181 gcggcgctgc cggtgccagc gccggccgcc gacccgatcg acatcgccgg ccggacggtc
      241 ctcgtcgtcg acgacaacgc caccaacctg cggatcctgg acgcgcagct cgggcggtgg
      301 cggatggccg tccgctcgac gcgctcaccg gcagaggcgc tggcgtgggt ccaggcaggc
      361 gcgtcgttcg acctcgcgat cgtcgacttc cacatgccgg tcatggacgg tctgacgttg
      421 ggtcacgagc tccaggcggt ccaccccgag gcaccgctcc cgatcgtgat cgtctcgtcc
      481 gtgggggcgc gcgaccggcg cgaatcgttc gtcgccgccg agctcaccaa gccggtgaag
      541 ccctcggcgc tgcatgatgc ggtgatgacg gccctcggcc gcgtcacccg tcccgaggcc
      601 gtcccggtcg caaaggcgag cccggacgcg cgcctcgccg aagaccggcc gcatcgcatc
      661 ctgctcgcgg aggacaacgc ggtgaaccag aagctcgcgc tccgcctgct cgagcgcatg
      721 ggctacacgg ccgacgtcgc ggagagcggc caggaagtcc tcgatgcgct cgagcgtgac
      781 ccgtacgacc tcgtgctgat ggacgtccag atgccggaga tggacggcct cgaggcgacc
      841 cgacgggcgc gcgcccggtg gcgcgaacgg tccctgtgga tcatcgcgat gacggccaac
      901 gccatggagg gtgatcgcga gatgtgcctc gcggccggga tgaacgacta catctcgaaa
      961 ccgatccgcc cggacgtcct ggcgggcgcg ctcgcatcgg cgccggttgc cgcggagaca
     1021 tcggtcgcac ggggccaggc ccgtggctga gacgctggac gccgccgtcc tggcggagct
     1081 gctcgaggcg gtcggcgacg atcccgcctt cgtcgacgag ctggtggaca cgttcctcgc
     1141 cgaggcgcct gggttcatga gcgcaatcga cggagccgtc gccgcaggcg atgccggcgc
     1201 gctcctcgtg cccgcccaca cgctcaaggg caacgcgtcc accatcggag cggggcggct
     1261 cgcggagatc tcgcgcaccc tcgaggagcg cgcgcggcgc ggggacctgg acggcgccgg
     1321 ctccgatgcc gcggcggctc gagctgagct ggcacacgtg gtcacggcgc tcgacgcaca
     1381 gcgcgccagg cggtggacgg cgtgagcgag ggcggccgga tcctcgtcgt cgacgacagc
     1441 cggctcaacc gccagacgct ggcccggctg ctgggcgggc tcggccacga ggtggtcgag
     1501 gcggaggatg gccgcgtcgc gctcgagctc ctcggcgagg acggtgccgg gatcgacgtc
     1561 gtcctgctcg atctcgtcat gcccgagctc gacggcttca gcgtgctggc cgcgatccgg
     1621 gcgaacccgg ccctcgcgcg catcccggtc atcgtcgtgt ccggcctcga cgacctcgac
     1681 gggatcgtgc gctgcgtcga gatgggggcc gccgactacc ttccgcggcc gatcaagccg
     1741 acgctgctcc gcgcgcgggt cgccacgtcc ctcgccgaga agcggctgcg cgacgacaac
     1801 gcccgcctcc tcgccacggt ggatcgccag cgcgaggagc tgtcgcgctt cctgtcgccg
     1861 caggtggccg ctctcgtgtc gacgcccgag ggcgagcagc tgctcgccgg gcatcggcgg
     1921 gtcgtcaccg cggtgttctg cgacctgcgc ggctacacgg cgttcggcga gatcgccgag
     1981 ccggaggagc tgctcgacgt cgtccgcgag taccacgcgg cgatgggccg gcggatcatc
     2041 gagttcg
//
LOCUS       DBNHXO010000453         4001 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_340770, whole genome shotgun sequence.
ACCESSION   DBNHXO010000453 DBNHXO010000000
VERSION     DBNHXO010000453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_340770"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..1115
                     /gene="speB"
                     /locus_tag="AC162A_09115"
     CDS             45..1115
                     /gene="speB"
                     /locus_tag="AC162A_09115"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HIR5011042.1"
                     /translation="MTDRPQPQGDVDFAARNGWDRAYETYDDFDLPTYVGLPSFMKLP
                     WIPDAAELRARNVDVAIVGAPFDDAVSHRSGARFGPRAIREAQYTSGSIHSLQQGNEP
                     FEILTVVDAGDANIVPSWIDRAHALIYRKVREVAATGAIPIVLGGDHSITWPSATAVA
                     DVRRPGSIGIVHFDAHADTAMDDWGVLAGHGTPMRRLIESGAVLGRNFVQVGLRGYWP
                     PVETFEWMQEQGMRWHLMREIEERGAEAVIDDAIAEALDGPDAVYISVDIDVIDPGIA
                     PGTGTPEPGGLLPREVLRAVRRIAAAVDIAGMDIVEVSPPYDHAEVTAMAANRIALEL
                     ISALAAKRQAGDPVRFAGPGTA"
     gene            1147..1929
                     /locus_tag="AC162A_09120"
     CDS             1147..1929
                     /locus_tag="AC162A_09120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5011043.1"
                     /translation="MTSESAAEALARLYDLDLSEDPGDLDLYLALAGRTGGPVLELAV
                     GSGRLAVPLAISGLAVTGVDVDPAMIARARAAADRAGATVARRVRLVRDDARTVRLPD
                     AGDFRLAVIPLNSIFLMGTRREQGQAIATLAAHLAPGGLAVVDAWLPDADDLARYDGR
                     LVLEWVREDQDSGHTVTKTGSAIYDPGSAMVRLTTVFEAGIAGQPAVRWVRVDMLHLV
                     GPDELVAFAEAAGLEVETMAGDYGLGPLELGADRVVMVARRP"
     gene            2056..3195
                     /locus_tag="AC162A_09125"
     CDS             2056..3195
                     /locus_tag="AC162A_09125"
                     /inference="COORDINATES: protein motif:HMM:NF025001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE/TadZ family protein"
                     /protein_id="HIR5011044.1"
                     /translation="MPDQLRLLVVEDVPQVAQYIRGLLNTQSTIKLLDVVTDGSRVAG
                     LVSELRPDVVLIDALLQGRVKGMALVDQLNQAGGGIPIIVLTVPQQPVAVDPRQGIHG
                     VLSMPFTGYELITRLQQVHQAAAASEATGASRMITIFAPKGGVGKTTLAFNLAVSLGQ
                     LGPRTVLIDGSLQFGDLRALLKVPIDAPSLLDLPTDRIQESDLQDVLWRDPSGIDILL
                     APPRVEMAEMVTVRDLEKTLSLLRRVYDVVVVDTNAVVNDINLSFLDASDIILEIVTY
                     DSTTIHSTMVMADAFRMIGYPPTKVRYLVNRSDSTGGFDPETLSRALGRQPEHLVASG
                     GALVVRANNEGIPFVLADPSAQISQDLARVASELVGRVPAASSRR"
     gene            3207..>4001
                     /gene="murI"
                     /locus_tag="AC162A_09130"
     CDS             3207..>4001
                     /gene="murI"
                     /locus_tag="AC162A_09130"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR5011045.1"
                     /translation="MSDPRPIGVFDSGVGGLTVLREILRRSPRESTIYLGDNARAPYG
                     VRSDEEVRAFSAEALDELASRDVKALVVACNTSTAVALRDLRARYDLPVLGVVRPGAA
                     AAALATRNRRVGVIATPATVRSHAYFNAIKDENPAIEVYEHATPRFVPLVEAGIVSGP
                     ELEAVIAEDLAPLLGERDGAGEFVFPRPPSSKIDTLLLGCTHYPLLRPVLAAAVTDQV
                     AIVDSATATASSLAELLSVNGLEVPGANPSPDADPGMYGLELPAEIY"
BASE COUNT          536 a         1479 c         1410 g          576 t
ORIGIN      
        1 gtgcctcgac ccgttcgacg ggctgccgcc cccggaggcc gatggtgacc gatcgtcccc
       61 aaccccaggg tgacgtcgac ttcgcggcgc gcaacggctg ggatcgcgcg tacgagacct
      121 acgacgactt cgacctgccg acctacgtcg gcctgccctc gttcatgaag ctgccctgga
      181 tccccgacgc ggcggagctc cgggcccgca acgtggacgt ggcgatcgtc ggcgccccgt
      241 tcgacgacgc ggtgagccac cggtccggcg ctcgcttcgg cccgcgggcg atccgcgagg
      301 cgcagtacac gtcgggctcg atccactccc tccagcaggg caacgagccg ttcgagatcc
      361 tgaccgtggt cgacgccggc gacgccaaca tcgtcccgtc gtggatcgac cgcgcccacg
      421 ccctgatcta ccgcaaggtg cgcgaggtcg cggcgacggg ggcgatcccg atcgtcctgg
      481 gcggcgacca ctcgatcacc tggccttcgg cgacggcggt cgcggacgtc cggcgccccg
      541 gcagcatcgg gatcgtgcac ttcgacgctc acgccgacac ggcgatggac gactggggcg
      601 tgctcgccgg acacggcacc ccgatgcgac ggttgatcga gtcgggcgcc gtcctcggcc
      661 gcaacttcgt ccaggtcggg ctccgcggct actggccgcc cgtcgagacc ttcgagtgga
      721 tgcaggagca gggcatgcgc tggcacctga tgcgcgagat cgaggagcgg ggcgccgagg
      781 cggtgatcga cgatgccatc gccgaggcgc tcgacggccc ggacgcggtc tacatctcgg
      841 tcgacatcga cgtgatcgac cccggcatcg cgcccggcac cggcaccccc gaaccgggcg
      901 gcctgctgcc gcgcgaggtg ctgcgggcgg tccgccggat cgccgcggcc gtggacatcg
      961 ccggcatgga catcgtggag gtctcgccgc cgtacgacca tgccgaggtc acggcaatgg
     1021 ccgccaaccg gatcgcgctg gagctcatct ccgcgctggc ggccaagcgg caggccggcg
     1081 acccggtgcg cttcgccggt cccggcaccg cgtgaccgcc cgctcggtgg cggcggaccc
     1141 gccgcgatga cctcggagtc ggccgccgag gcgctcgcgc gcctgtacga cctcgacctg
     1201 tccgaggacc cgggcgacct cgacctgtac ctcgcgctcg ccggtcggac cggaggcccg
     1261 gtgctggagc tggcggtcgg ctcgggccgg ctggccgtgc cgttggcgat ctcggggctc
     1321 gccgtgaccg gcgtcgacgt cgatccggcg atgatcgccc gggcgcgcgc cgcggccgac
     1381 cgagccgggg cgaccgtcgc ccgccgcgtc cgcctggtgc gggacgacgc ccggacggtc
     1441 cgccttccgg acgccgggga cttccgcctg gcggtgatcc ccctcaactc catcttcctc
     1501 atgggcacgc gccgcgagca agggcaggcg atcgcgaccc tggcggccca cctcgcgccg
     1561 ggcggcctcg cggtggtcga cgcctggctc cccgacgccg acgacctggc ccgctacgac
     1621 gggcgcctcg tgctcgaatg ggtccgcgag gatcaggact ccggccacac ggtgaccaag
     1681 accggcagcg cgatctacga cccggggtcg gccatggtcc gcctcacgac cgtgttcgag
     1741 gccgggatcg cgggtcagcc cgccgtgcgc tgggtgcgtg tggacatgct gcacctggtc
     1801 gggcccgacg agctggtggc attcgccgag gcggccgggc tcgaggtgga gaccatggcg
     1861 ggcgactacg gcctcgggcc gctcgagctc ggggccgacc gcgtcgtgat ggtcgcccgg
     1921 cgaccctgat ccgagcggcc cggaactcgc gccgggagcg ctcccgtcga ggtcggccat
     1981 cccccgtgcg gccgttcggc atgggacctt cggggccggg agaagggacc gggactgcgt
     2041 ggtagagtcg ggccgatgcc ggatcagctc cgcctcctcg ttgtcgagga cgtcccccag
     2101 gtcgctcagt acatccgcgg actcctgaac acgcagtcga cgatcaagct gctcgacgtc
     2161 gtcaccgacg ggtcgcgggt cgccggactc gtgtcggaat tgcgcccgga cgtcgtgctc
     2221 atcgacgccc tgctccaggg ccgggtgaag ggcatggcgc tcgtcgacca gctcaaccag
     2281 gcgggtggcg gcatcccgat catcgtgctc accgtgcccc agcagccggt cgcggtcgat
     2341 ccgcgccagg gcatccacgg cgtcctgtcg atgccgttca cgggctatga gctgatcacg
     2401 cgcctgcagc aggtccacca ggccgccgcg gcgagcgagg cgacgggcgc gtcgcggatg
     2461 atcacgatct tcgcgcccaa aggcggggtg ggcaagacga ccctggcctt caacctcgcg
     2521 gtctccctgg gccagctcgg gccgcgcacc gtgctgatcg acggcagcct ccagttcggc
     2581 gacctccgcg cgctgctcaa ggtgccgatc gacgcgccgt ccctgctcga cctcccgacc
     2641 gaccggatcc aggagtcgga cctccaggac gtcctgtggc gcgacccgtc gggcatcgac
     2701 atcctgctcg cgccgccgcg cgtcgagatg gccgagatgg tgaccgtgcg cgacctcgag
     2761 aagacgctgt cgctgctgcg ccgggtctac gacgtcgtcg tggtcgacac caacgcggtc
     2821 gtcaacgaca tcaacctctc gttcctcgac gcgtccgaca tcatcctcga gatcgtgacc
     2881 tacgactcga cgacgatcca cagcacgatg gtgatggccg acgccttccg gatgatcggc
     2941 tacccgccga ccaaggtgcg ctacctcgtg aaccggtccg actcgacggg cggcttcgat
     3001 cccgagaccc tctcacgggc cctgggacgc cagcccgagc acctcgtcgc ctcgggcggc
     3061 gcgctcgtcg tgcgggccaa caacgagggc atcccgttcg tgctcgcgga cccctcggcg
     3121 cagatcagcc aggacctggc gcgcgtcgcg agcgagctgg tgggtcgggt gcccgccgcc
     3181 tcgagccggc ggtagtctcg gaagacgtgt cggacccgcg gccgatcggt gtcttcgact
     3241 cgggcgtagg tggtctcacc gtcctccgcg agatcctccg ccggtcgccg cgcgaatcca
     3301 cgatctacct cggcgacaac gcccgcgcgc cgtacggcgt gcgctcggac gaggaggttc
     3361 gggcgttctc cgcggaggcg ctggacgagc tggcctcgcg tgacgtcaag gcgctggtcg
     3421 tcgcctgcaa cacgtcgacc gcggtcgcgc tgcgcgacct gcgggctcgg tacgaccttc
     3481 cggtgctggg cgtcgtgcga ccgggcgccg cggcggccgc gctggcgacc cggaaccggc
     3541 gcgtcggcgt catcgccacc ccggcgacgg tccgatcgca cgcctacttc aacgcgatca
     3601 aggacgagaa cccggcgatc gaggtctacg agcacgccac cccgcgcttc gtgccgctgg
     3661 tcgaggcggg catcgtgtcc ggcccggagc tcgaggcggt gatcgcggag gacctcgcgc
     3721 cactcctggg cgagcgcgac ggagcggggg agttcgtctt tccgaggcct ccgtcatcga
     3781 agatcgacac gctcctcctc ggctgcacgc actacccgct gctgcgtccc gtcctggcgg
     3841 ccgcggtcac ggaccaggtg gcgatcgtcg actccgccac ggcgacggcc tcgtcccttg
     3901 ccgagctgct gtcggtgaac ggcctcgagg tgccgggcgc gaatccgagc cctgacgccg
     3961 accccgggat gtacggtctc gagctccccg ccgagatata c
//
LOCUS       DBNHXO010000454         4605 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4506080, whole genome shotgun sequence.
ACCESSION   DBNHXO010000454 DBNHXO010000000
VERSION     DBNHXO010000454.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4506080"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1327
                     /locus_tag="AC162A_09135"
     CDS             32..1327
                     /locus_tag="AC162A_09135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HIR5011046.1"
                     /translation="MRLLHLLWPHLPLRLARSRLGSPGSDGISASWPPGPIVLGGQPW
                     TDGTVVDADPLARALGVRRGIPLGSAHRLAPEATFLDLAPEADRDAVEAACERLAAFS
                     PGISGTSDVVDAAFGRLAVHVDGLTRLWGIEETLVARIGEALAPLLPGPARAGVAGTS
                     FAASVAAAHASEGGPPLLVPPGDDDRFLAPYPARLLTRDANVRARLARFGLRTIGSVA
                     ELPRSALVARFGDEGALLHARARGEETERFRPRRNAERLALALPLDPPAEGIEAVRFV
                     LHRLAATLADQLQARGQAAGLARLVVSHDLAFARRGVPAAIHLEQRLPEPTADAEAIE
                     RLLFARLEKAPPPAAVARLELELARVEVATGQQLPLFTPQAARDARLAWQLARLAIAF
                     GTDRVRRIEITDPEAPIAESRWRWVPVAGAAVTGDEAPR"
     gene            1324..1572
                     /locus_tag="AC162A_09140"
     CDS             1324..1572
                     /locus_tag="AC162A_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011047.1"
                     /translation="MTRLLREHPAIDAELDADGRLVAIRWNGRREAVEVCNRWRVEES
                     WWQEPILRDYFKVVGRSWLALVYLDRGAGTWHLERLFD"
     gene            complement(1828..2403)
                     /locus_tag="AC162A_09145"
     CDS             complement(1828..2403)
                     /locus_tag="AC162A_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011048.1"
                     /translation="MRSPRSRLAVVALVTGVVLTLAACGDATRPPATVIASLPAPTSD
                     APASPTPAAPGEPSPTAVPGGQTIDPEPPPTQRPTTTKTDWGVILDAVPDDFPRYPGA
                     AGIDSPDEPVSEALETADGVEEVATWYREALEAAGYMTIDLSAPLEDGSRVLDSQTDI
                     PECRVQTTFRPEGEATMITVLFAAGCVALGG"
     gene            complement(2595..2726)
                     /locus_tag="AC162A_09150"
     CDS             complement(2595..2726)
                     /locus_tag="AC162A_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011049.1"
                     /translation="MIAPLTIHPDRRLALELLVRAAAIALAAIVILGFLPALAEAVG"
     gene            2849..>4605
                     /locus_tag="AC162A_09155"
     CDS             2849..>4605
                     /locus_tag="AC162A_09155"
                     /inference="COORDINATES: protein motif:HMM:NF014826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR5011050.1"
                     /translation="MAHPAYAELHCHSHFSFLDGASAPDDLVARAVELGLSGLAITDH
                     QGLYGAVRFSTAAEAAGLHPVIGIEIELVDAAVPDPAGIVVPSRRAWRPGRRPRVVIE
                     PPVVVAGRPARPMPDRARLPGHRAVVKEDHRGIGEAQRGPHLVLLARDATGWRSLCRM
                     VSRANLAGTKAVPKFTQALRTEHADGLIALSGCREGELSRRLRAGDREGARALAERYA
                     GLFGRGDSAAASGFVLELSHHLLPDDDWLVAETARLAGELGLPVVVTNDVHYDRPEGR
                     ELQDVLAAIRHGRSLPELRDLRRPDGESYLKGAAELLALPPGEPSTAAADPVLARAWR
                     EGIAASAEIAAGCRVELTFERYRFPGFDVPRGETPFSHLSSLCWEGAKRRYHPFTPAV
                     VRQLAHELDVIEKTGLAEFFLICWDLMRFAKSRRIPAQGRGSAADSIVAYVLGITRVD
                     PIRHELLFERFINEGRTAYPDVDIDFSSERREEVIQYVYERYGTEHTGMVCNLVTYRA
                     RSAVREVGYALGFPRPLVDRVAKALETYDSVMVRRDLEAEGGFAQFFARPGEVPGEAA
                     APVAASLAAAHGLTDAMGQL"
BASE COUNT          584 a         1619 c         1776 g          626 t
ORIGIN      
        1 ctgaaaggcc cccctccatc ggccgatacc catgcgactc ctgcacctgc tctggcccca
       61 cctgccgctc cggctcgccc ggtcccgcct cggttctccg gggagcgacg gcatctccgc
      121 ctcgtggcca cccggaccga tcgtcctcgg cggtcagccc tggacggacg ggacggtggt
      181 cgacgcggac ccgctcgcac gggcgctcgg cgtccggcgg gggatcccgc tggggagcgc
      241 ccaccggctc gcgcccgagg cgaccttcct cgacctcgcg cctgaggcgg accgggacgc
      301 cgtcgaggcc gcctgcgaac ggctcgccgc gttcagcccc gggatctccg gcacgagcga
      361 tgtcgtggac gctgcgttcg ggcgcctggc ggtccacgtc gacgggctga cgcggctgtg
      421 ggggatcgag gagacgctcg tcgcccgcat cggcgaggcg ctcgcgcccc tgctgccggg
      481 tccggcgcga gccggggtcg cgggcacgag cttcgcggcc agcgtcgccg cggcgcacgc
      541 gtccgagggc ggaccgccgc tcctcgtccc gcccggcgac gatgaccggt tcctcgcccc
      601 gtatcccgcc cgcctgctga cgcgggatgc caacgtccgg gcacgcctcg cgcggttcgg
      661 gctgcgcacg atcggctcgg tggcggagct gccgcgctcg gcgctggtgg cgcgcttcgg
      721 cgacgagggg gcgctgctgc acgcgcgggc ccggggcgag gagaccgagc ggttccggcc
      781 gcggcgcaac gcggagcgcc tcgcgctggc gctgccgctc gatccaccgg ccgagggcat
      841 cgaggccgtc cggttcgtgc tccaccggct cgcggcgacg cttgcggacc agctgcaggc
      901 gcgaggccag gccgccgggc tggcgcggct ggtcgtgagc cacgacctcg cgttcgcccg
      961 gcgcggcgtc ccggcggcga tccacctcga gcagcgcctg cccgagccca ccgccgacgc
     1021 cgaggcgatc gagcgcctgc tgttcgcccg cctggagaag gcgcccccgc cggccgccgt
     1081 cgcccgcctg gagctggagc tggcacgggt cgaggtggcg accggtcagc agctgccact
     1141 gttcacgccg caggccgcgc gcgacgcccg gctggcctgg cagctggcga ggctcgcgat
     1201 cgcgttcggg acggaccggg tccggcggat cgagatcacg gaccccgagg cgcccatcgc
     1261 cgagtcgcgc tggcgctggg tcccggtcgc gggtgccgcg gtgacgggcg acgaggcccc
     1321 ccgatgaccc ggctgctgcg ggagcacccg gcgatcgacg cggagctgga cgcggacggg
     1381 cggctggtcg cgatccgctg gaacggacgc cgcgaagcgg tggaggtgtg caaccgctgg
     1441 cgggtcgagg agtcgtggtg gcaggagccg atcctgcgcg actacttcaa ggtggtcggg
     1501 cggagctggc tggccctcgt gtatctcgac cgcggcgccg ggacgtggca cctggagcgg
     1561 ctgttcgact gagggcgaga acgcgagcga tggcggcgac cgaccggagg tcgaagcgtc
     1621 agcggacggc gggtgacccg agcgcgatcg agacgaccag cagcacggtg aacacggcta
     1681 tcacgagtgc cagtgccccg aaatcgcgcg gtcgagccgt ctgcatggct gcgtgtcccc
     1741 atccccggac cggtcccggc cggtcgcctt cagcatagac gcgatcatgc cgattcgtgt
     1801 tgcgatcttg gtcaacaacc gggaatctca gccgcccagc gcgacgcacc cggcggcgaa
     1861 caggacggtg atcatcgtcg cttcaccctc gggccggaac gtcgtctgga cgcggcactc
     1921 ggggatgtcg gtctgcgagt cgagcacccg cgaaccgtcc tcgagcgggg ccgacaggtc
     1981 gatcgtcatg tagccggcag cctcgagcgc ctcccggtac caggtggcga cctcctccac
     2041 gccgtcggct gtctcgaggg cctcgctgac gggctcgtcc ggcgagtcga tgcccgcggc
     2101 acccgggtag cgcgggaagt cgtccgggac ggcgtcgagg atcacgcccc agtcggtctt
     2161 cgtcgtggtc gggcgctggg tcggcggcgg ttcggggtcg atcgtctggc cgccggggac
     2221 ggcggtcgga gacggctcgc ccggcgcagc gggcgtgggc gaggccggcg cgtcagacgt
     2281 cggtgccgga agcgacgcga tcacggtggc gggcgggcgg gttgcgtcgc cgcaggcagc
     2341 cagggtcaag acgacaccgg tcacgagggc gacgacggcg aggcggctgc gaggggaacg
     2401 catggccggc ccatcgtagc gggatgcctg ggcgagccgg aaagagcgag gacccccgcc
     2461 gccccagcgg cgagggtcct cgggggttgc caccggactg cccagggtcc ggcggcaggt
     2521 cgacgagcgc catcgcaacg ttcgtcgccg tgggtgttcc gtgagacctc aggtcatcgg
     2581 gaacgttggc gtgatcaccc gaccgcctcc gccagtgccg ggaggaatcc caggatcacg
     2641 atcgccgcca gcgcgatggc tgctgcgcga acgagcagct cgagggcgag gcgacggtcg
     2701 ggatggatgg tcagcggggc gatcacgggc attagacgcg caaccccccg attcggttgc
     2761 gtcggcaggc atcgatcagg taacggtcgt cttgcttcga atcgaacatc tgttctatca
     2821 tctcggacct gcccccggag accgtcgcgt ggcccatccc gcctacgccg agctgcactg
     2881 ccacagccac ttctcgttcc tcgacggggc atcggccccg gacgatctcg tcgcgcgggc
     2941 cgtcgagctc gggctgtccg ggctggcgat caccgatcac cagggcctgt acggcgcggt
     3001 ccgattctcg accgccgcgg aggcggccgg gctgcacccg gtcatcggga tcgagatcga
     3061 gctcgtcgac gccgcggtgc cggatccggc cgggatcgtc gttccgtcga ggcgcgcctg
     3121 gcggcccggc cgcaggccgc gggtggtcat cgagccaccc gtcgtcgtcg cggggcgacc
     3181 cgcccgccca atgcctgacc gtgcccggct gcccggtcac cgcgcggtcg tcaaggagga
     3241 ccaccggggg atcggggagg cacagcgcgg cccgcacctg gtgctcctcg cccgcgacgc
     3301 gacgggctgg cggagcctgt gccggatggt gtcgcgggcg aacctcgccg gcaccaaggc
     3361 cgtcccgaag ttcacccagg cgctgcggac ggaacacgcg gacgggctga tcgcgctgtc
     3421 gggctgtcgg gagggcgagc tgtcgcgacg gctgcgggct ggtgatcggg agggcgcgcg
     3481 ggcgctcgcg gagcggtacg cgggcctgtt cggtcggggc gattcggcgg ccgccagcgg
     3541 cttcgtgctc gagctgtcgc accacctgct ccccgacgac gactggctcg tcgcggagac
     3601 ggcgcggctg gccggcgagc tggggctgcc ggtggtggtg accaacgacg tccactacga
     3661 ccggccggag gggcgcgagc tccaggatgt gctggccgcg atccggcacg ggcgctcgct
     3721 gcccgagctg cgggacctgc gccggcccga cggggagtcg tacctcaagg gcgcggccga
     3781 gctgctcgcg ctgccgccgg gagagccgtc gaccgcggcc gccgatcccg tgcttgcccg
     3841 ggcgtggcgc gaggggatcg cggcatcggc ggagatcgcc gcgggctgcc gggttgagct
     3901 caccttcgag cgctaccgct tcccgggctt cgacgtgccc aggggcgaga cgccgttcag
     3961 ccacctctca tcgctgtgct gggaaggggc gaagcgccgc taccacccgt tcacgcccgc
     4021 ggtcgtgcgc cagctggccc acgagctgga cgtgatcgag aagacgggcc tcgccgagtt
     4081 cttcctgatc tgctgggacc tgatgcggtt cgcgaagtcg cgcaggatcc cggcccaggg
     4141 acgggggagc gccgcggact cgatcgtggc gtatgtcctg gggatcacgc gggtggatcc
     4201 catccgccac gagctgctgt tcgagcggtt catcaacgag ggccggacgg cgtacccgga
     4261 cgtggacatc gacttctcct ccgagcggcg ggaggaggtc atccagtacg tctacgagcg
     4321 ctacgggacc gagcacacgg ggatggtctg caacctcgtc acgtaccggg cgcggtccgc
     4381 ggtgcgcgag gtgggctacg cgctggggtt cccgcggccg ctcgtggacc gggtcgcgaa
     4441 ggcgctcgag acgtacgaca gcgtgatggt ccggcgggac ctcgaggcgg agggcggctt
     4501 cgcgcagttc ttcgcccggc cgggggaggt gcccggggag gctgcggcac cggtggctgc
     4561 gtccctggcc gccgcccacg gcctgaccga cgcgatgggc cagct
//
LOCUS       DBNHXO010000455         4091 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4307727, whole genome shotgun sequence.
ACCESSION   DBNHXO010000455 DBNHXO010000000
VERSION     DBNHXO010000455.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4307727"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="AC162A_09160"
     CDS             <1..264
                     /locus_tag="AC162A_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010898630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR5011051.1"
                     /translation="AEESFATGLLEYPQYTRPATFEGESVPPILVSGHHEQVRRWRLR
                     ESLRRTLDRRPDLLEGRDLSAEERALLDDVHADRDAADGAPDA"
     gene            382..726
                     /gene="rplS"
                     /locus_tag="AC162A_09165"
     CDS             382..726
                     /gene="rplS"
                     /locus_tag="AC162A_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR5011052.1"
                     /translation="MNVLNEIVQDQLRTDLPVITPGDTVKVAAKVVEGNRERVQVFEG
                     LVMRMRNSGIASTITVRRIASGVGVERTFKLHSPRIDRIEVVRHGQVRRAKLYFLRDR
                     VGKAATTLRERR"
     gene            complement(723..1553)
                     /locus_tag="AC162A_09170"
     CDS             complement(723..1553)
                     /locus_tag="AC162A_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR5011053.1"
                     /translation="MDRAFAQVDVFTTEPGLGNPLAVVLDGSGLSSDEMQRFARWTNL
                     SETTFLLPPTDPAADYRARIFHPEAELPFAGHPTLGSCHAWLEAGGTPRHADRIVQEC
                     GAGLVEVRREGDVLSFAAPPLLRSGPVDEADVLAIAEGLGITREAIVDATWADNGPGW
                     AAILLASADEVLAIRPGLVPFDIGIVGPYPDGSPAALELRAVWPQHGATVEDPVTGSL
                     NASVAGWLISTGRIRAPYTASQGTAIGRAGRVLITQDADGTVWTGGRSATLVRGTVTL
                     "
     gene            1593..2342
                     /locus_tag="AC162A_09175"
     CDS             1593..2342
                     /locus_tag="AC162A_09175"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR5011054.1"
                     /translation="MAVIVPTLRWERPLWRDGATRIVGVDEVGVAPTCGAVVAAAVIM
                     SPNCHRIPGVRDSKTLSAAQRERLAPIIRRRAVAVGLGAASVADIDRLNIYHATHLAM
                     RRAIARVGGHDHVLVDGLRIADFEAEVGPYTAIVDGDARVYSIACASVVAKVVRDRMM
                     HALAVRYPQYGWDRNAGYATREHRDAIRAHGLTPHHRKSWQALQVLLAGDQLGLFDVD
                     DDLADEGDLVAVPIGGFDAADQIAVGAVGDR"
     gene            2378..2776
                     /locus_tag="AC162A_09180"
     CDS             2378..2776
                     /locus_tag="AC162A_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR5011055.1"
                     /translation="MTYPAGPAQRVGAAAERFIASRLIGEGWEILARNARIGRDELDL
                     IGIDPGPPRTLVVVEVRWRGRRDFGLAEESLDHRKRAALRRAIGALIGAGGLPDGKAL
                     PRLPLRVDLVAVDPGPDGEPSVRHHRGIRP"
     gene            2990..3913
                     /gene="rpsB"
                     /locus_tag="AC162A_09185"
     CDS             2990..3913
                     /gene="rpsB"
                     /locus_tag="AC162A_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR5011056.1"
                     /translation="MPTVSMRQLLEAGVHFGHQTRRWNPKMRPYIFAERNGIHIIDLA
                     QTVKRLDTALEFVTETVARGESVMFVGTKKQAQEPVMQEAMRSGQPYVTKRWLGGMMT
                     NFVTIKKRIGLLDQLEARQLAGDFDRLPKKEAALLTEELNKLNAMLGGIRKMKRLPGA
                     IFIVDPHRERIAVTEANKLEIPVIGTGDTNVDPDELDFIIPANDDAIRAIRLLCQLVA
                     DAAIEGSQLRAARASSEPEAPEQQAIDEPEFDETAAADLVAQLAAGGTLSFDPELDDE
                     DLLPGSPRAEEPAAADSPADDATPAADAPAD"
BASE COUNT          612 a         1519 c         1446 g          514 t
ORIGIN      
        1 gccgaggagt cgttcgcgac cgggctgctc gagtacccgc agtacacgcg gccggcgacg
       61 ttcgagggcg agtcggtgcc gccgatcctg gtctcgggcc accacgagca ggtccgccgc
      121 tggcggctgc gggagtccct gcgccggacg ctggaccgcc gtcccgatct gctggagggg
      181 cgcgacctgt ccgccgaaga gcgagcgctt ctcgacgacg tgcacgccga ccgcgacgcg
      241 gcggacggcg ccccggacgc ctgacacccg tcacgcgccg tgctattctc cgccgtcggc
      301 cccgccgttt tcggcctgcc gcccgtctga attgggccca ccgcagccgc ggctgccgtc
      361 gatcgaacaa ggaccgccac cgtgaacgtg ctcaacgaga tcgtccagga ccagctgcgc
      421 accgacctcc ccgtgatcac cccgggcgac accgtcaagg tcgccgcgaa ggtcgtcgag
      481 ggcaaccgcg agcgcgtcca ggtcttcgag ggcctcgtca tgcgcatgcg caacagcggc
      541 atcgcgtcga cgatcaccgt ccgccggatc gcctcgggtg tcggggtcga gcgcacgttc
      601 aagctgcaca gcccgcgcat cgaccggatt gaggtcgtcc gccacggcca ggtccgccgc
      661 gccaagctgt acttcctgcg cgaccgcgtg ggcaaggccg ccacgacgct ccgcgagcgc
      721 cgctagagcg tcaccgttcc ccggacgagc gtcgcgctgc gaccaccggt ccagacggtc
      781 ccgtccgcgt cctgggtgat caggactcgt cccgcgcgcc cgatcgccgt cccctgtgac
      841 gccgtgtaag gcgcccggat ccgccccgtc gagatcagcc atcccgcgac cgacgcgttg
      901 aggctgcccg tgaccgggtc ctcgacggtc gccccgtgct gcggccagac cgcgcggagc
      961 tccagcgccg ccggcgatcc gtcgggatac ggccccacga tgccgatgtc gaacggcacc
     1021 agaccgggac ggatcgcgag gacctcgtcg gcgctcgcca gcaggatcgc cgcccagccc
     1081 ggaccgttgt ccgcccacgt cgcgtccacg atcgcttcgc gcgtgatgcc gagcccctcg
     1141 gcgatcgcca gcacgtccgc ctcgtcgacc ggcccggagc gcaggagcgg cggcgccgcg
     1201 aagctgagca cgtccccctc gcggcggacc tcgaccagcc ccgcgccgca ttcctggacg
     1261 atccggtccg catgccgggg cgtgccgccc gcctcgagcc aggcgtggca cgagccgagg
     1321 gtcgggtggc ccgcgaacgg cagctcggcc tcggggtgga agatgcgggc gcggtagtcg
     1381 gccgccggat cggtcggcgg gagcagaaac gtcgtttccg agaggttcgt ccaccgggcg
     1441 aagcgctgca tctcgtcgct cgacagcccg ctgccgtcca ggacgaccgc gagcgggttg
     1501 ccgagcccgg gctcggtggt gaacacgtcg acctgtgcga acgcgcgatc catgggtccc
     1561 gagtctgcca tggcgcggta ggctatgggc cgatggccgt gatcgtcccg acgctgcgct
     1621 gggagcgccc cctgtggcgc gacggcgcga cccgcatcgt gggcgtggac gaggtcgggg
     1681 ttgcgccgac ctgcggtgcc gtggtcgcgg cggccgtgat catgtccccc aactgccacc
     1741 ggatcccggg cgtgcgcgac tcgaagacgt tgtccgccgc ccagcgcgag cgattggccc
     1801 cgatcatccg gcggcgcgcc gtcgccgtcg gcctgggcgc cgcgtccgtg gcggacatcg
     1861 accgcctcaa catctaccac gcgacccacc tcgcgatgcg ccgcgcgatc gcgcgcgtcg
     1921 gcggccacga ccacgtgctg gtcgacgggc tgcggatcgc ggacttcgag gcggaggtcg
     1981 ggccgtatac ggcgatcgtc gacggggacg cgcgggtcta ctcgatcgcg tgcgcgtcgg
     2041 tcgtcgccaa ggtggtccgc gaccggatga tgcacgccct cgcggtgcgc tacccccagt
     2101 acggctggga tcgcaatgcc ggctacgcca cccgtgagca ccgtgacgcg atccgggccc
     2161 acgggctgac accccaccac cgcaagagct ggcaggcgct ccaggtgctg ctcgccgggg
     2221 accagctggg gctgttcgac gtcgacgacg atctcgcgga tgagggcgac ctggtggcgg
     2281 tcccgatcgg tggcttcgac gcggcggacc agatcgcggt gggcgccgtc ggcgaccgat
     2341 aagcgccggt tgagtggccg cggccatcat cgggcgcatg acctacccgg ccggccccgc
     2401 ccagcgtgtg ggtgccgccg cggaacggtt catcgcgagc cggctcatcg gggagggctg
     2461 ggagatcctg gcccgcaacg cgaggatcgg gcgcgacgag ctggacctga tcgggatcga
     2521 tcccggtccg ccgcgcacgc tggtggtcgt cgaggtccgc tggcggggcc ggcgggactt
     2581 cgggctggcc gaggagagcc tcgaccatcg caagcgggcg gcgctgcgcc gggcgatcgg
     2641 cgccctgatc ggggccggcg gactcccgga cgggaaggcc ctgccacgcc tgccgctgcg
     2701 cgtcgacctg gttgctgtcg accccggacc ggacggcgag ccgtcggtcc gccaccaccg
     2761 cgggatccgg ccatgactgg cgccggagcg ggaagcccgg ggacccgtgc tacactcccg
     2821 gccgccgcgg acacgccgtg gcgtaccagg cccacgccgg atcgaccggc atggagcaca
     2881 cacgcggatc acgatcccga gcgggacggt gccggacggg cagaggtcgc gagacctgcc
     2941 gacggccccg caggggatcc gcggaggcac aaccgcagga ggacacagcg tgcccaccgt
     3001 ctcgatgcgc cagctgctcg aagccggcgt ccacttcggc caccagacgc gccgatggaa
     3061 ccccaagatg cgcccgtata tcttcgcgga gcgcaacggg atccacatca tcgacctcgc
     3121 ccagacggtc aagcgcctgg acacggcgct cgagttcgtc accgagaccg tcgcccgcgg
     3181 cgagagcgtg atgttcgtcg gcaccaagaa gcaggcgcag gagccggtga tgcaggaggc
     3241 gatgcgctcc ggccagccgt acgtcaccaa gcgctggctg ggcggcatga tgacgaactt
     3301 cgtcaccatc aagaagcgga tcggcctgct cgaccagctc gaggcccggc agctcgccgg
     3361 cgacttcgac cggctgccga agaaggaagc cgcgctgctc accgaggagc tgaacaagct
     3421 caacgcgatg ctcggcggca tccgcaagat gaagcgcctg cccggcgcca tcttcatcgt
     3481 cgacccgcac cgcgagcgga tcgccgtgac cgaggccaac aagctcgaga tcccggtcat
     3541 cggcacgggc gacaccaacg tcgacccgga cgagctggac ttcatcatcc cggccaacga
     3601 cgacgcgatc cgcgccatcc ggttgctgtg ccagctggtc gccgacgcgg cgatcgaggg
     3661 ttcgcagctg cgcgccgcac gcgcctcgtc cgagcccgag gcacccgagc agcaggcgat
     3721 cgacgagccg gagttcgacg agaccgccgc ggccgacctg gtcgcccagc tggcggcggg
     3781 cggcacgctg tcgttcgatc ccgagctcga cgacgaggac ctcctcccgg gttcgccgcg
     3841 agctgaggaa ccggcagccg ccgactcccc ggctgacgac gcaaccccgg ccgcggacgc
     3901 gcccgccgac tgatccgtgg cgcggggcgg cggcgccgtc gaccgcccgc gcccacgacc
     3961 acgaacaacc tacggcacgc gccacctgcg cacgccgtac gctagacaca ggagcaccac
     4021 gcccatggca atcaccgccg aagccgtcag ggagctgcgc gagcgcaccg gcgcgggcat
     4081 gatggactgc a
//
LOCUS       DBNHXO010000456         2698 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4705022, whole genome shotgun sequence.
ACCESSION   DBNHXO010000456 DBNHXO010000000
VERSION     DBNHXO010000456.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4705022"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="AC162A_09190"
     CDS             <1..186
                     /locus_tag="AC162A_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIR5011057.1"
                     /translation="ERFRYLYEPDELREWVPRVMEAAGQARDTHVLMNNCYANYGSTN
                     ARELAAMLAEELAGASI"
     gene            266..799
                     /locus_tag="AC162A_09195"
     CDS             266..799
                     /locus_tag="AC162A_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5011058.1"
                     /translation="MEPDVRPAAPSAGLDTDLSAIYVAHAPGLVRWMISITHDGELAA
                     DLVQEAFLRLARELEAGRRPDNVPAWLAQVARNLAVSRARRVATAARYEDRLARPSAP
                     VDPATAALDAERSRAVHAALADLRPDDRTALLLAAEGHRNAEIAQQIGRTELATRALL
                     CRARRRLRPVLQGVAPV"
     gene            810..>2698
                     /locus_tag="AC162A_09200"
     CDS             810..>2698
                     /locus_tag="AC162A_09200"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5011059.1"
                     /translation="MGRPEDVPFVARSDELATVRTLLERAREGSSAVLVIGGEAGVGR
                     TRLVEAIADAARRDGFRVATGTCVRMDAGAMPYLAIIAALRALVAQGDPATVAASLGG
                     QRREIARLLPEVARLGVPVDRDTVGLPAVPAGPRAMPAPAVAPGSAGSTEREPLERLR
                     LFEAVTTWLARLADAAPLLLVIDDLQWVDPSTLDLLRALAPALAHGSVLVVSLRTDQP
                     LPGAVGATVAELVRDGATRLELAPFERNELERLVAAATGTDVGAVDPAALDHLTARSG
                     GNAFLALALIDAGLVSADAPYVGVPASLRDILDAELASLDDETLAMLRAAALEPGPLD
                     DAVLAGVLEVPVTAVGRAIREAHHAGVLAIGRGVGAERVPRFRHAIQREVLVDQLGHG
                     EQRSLHARYADVLESADLDPSRASAVALHRDGAGDGPRALAAHLAAMDAAERAFAFDA
                     AARHGTRAAALRRGIGVADPGTPDEAGLLGRASYAALLAGDAPAAAELARRALATTEV
                     DDARAAVLHDRIRWALWLEGDRAGSARELELAFERLGDAPPDALRAHLLAERAAMRTG
                     EADPRAALDLAQEAIGIARAVGAPDIEALGLGILGRALATHGRVDEGIGSLRAAVGIA
                     DRIGNL"
BASE COUNT          318 a          995 c         1035 g          350 t
ORIGIN      
        1 gagcggttcc gctacctgta cgagccggac gagctgcgcg agtgggtgcc gcgggtgatg
       61 gaggccgccg gccaggcccg cgacacgcac gtgctcatga acaactgcta cgcgaactac
      121 ggctcgacga atgcacgaga gctggcggcg atgctggccg aggagctggc gggagcgtcg
      181 atctaggtat cgcgcccgat gtggccgggg tcgccttaga cccagcgtcg gctgcaggtc
      241 attcctgcag ctggagcaag ccacgatgga acctgatgtc cgccccgccg caccaagcgc
      301 cgggctcgac accgacctct cggcgatcta cgtcgcccat gcgccgggcc tcgtgcgctg
      361 gatgatcagc atcacccatg acggtgagct cgccgccgac ctggtccagg aggcattcct
      421 gcggctcgcg cgggagctcg aggccggcag gcggcccgac aacgtccccg cctggctcgc
      481 gcaggtcgcc cgcaacctcg ccgtgagccg ggcgcggcgc gttgcgacgg cggcgcgtta
      541 cgaagaccgg ctcgcccggc cgtccgcgcc ggtggacccg gcaaccgcgg cgctcgacgc
      601 cgagcggtcg cgcgccgtcc atgcggcgct ggccgacctg cgcccggacg atcggacggc
      661 gctgctgctt gccgcggagg gccaccgcaa cgcggagatc gcccagcaga tcgggcgcac
      721 cgagctcgcg acccgtgccc tcctgtgccg cgcgaggcgg cggcttcggc cggtattgca
      781 gggggtcgcg cccgtatgat cccccggcgg tgggtcggcc cgaagatgtg cctttcgtcg
      841 cgcggtcgga cgagctggcg acggttcgga cgctgctgga gcgcgcccgt gagggatcgt
      901 cggccgtcct ggtgatcggt ggcgaggcgg gcgtcgggcg gacgcgcctc gtcgaggcca
      961 ttgccgacgc cgcccggcgt gacgggttcc gcgtggcaac cggcacgtgc gtccggatgg
     1021 acgccggcgc catgccctat ctcgcgatca tcgcggccct gcgggcgctc gtggcccagg
     1081 gcgacccggc aacggtcgcg gcgtcgctgg gtggccagcg ccgggagatc gcccggctcc
     1141 tgccggaggt ggccaggctc ggcgtgccgg tcgatcggga cactgtcggc ctcccggcgg
     1201 tcccagccgg cccacgggcg atgccggcgc cggccgtggc ccccggctcg gccgggagca
     1261 cggagcggga gccgctcgag cgtctccgcc tgttcgaggc cgtcaccacg tggctggcgc
     1321 ggctcgccga cgccgcgcca ttgctcctcg tcatcgatga tctccagtgg gtcgatccat
     1381 cgacgctcga tctcctgcgc gctctggcgc ccgcgctcgc gcatggctcc gtgctcgtcg
     1441 tcagcctgcg aacggaccag ccactgcccg gtgcggtggg cgcgaccgtg gccgagctcg
     1501 tgcgcgacgg cgcgacgcgc ctcgagctcg cgccgttcga acggaacgag ctcgaacgcc
     1561 tcgtcgcggc cgcaacgggg acggacgtcg gcgccgtcga cccggcggcg ctcgatcacc
     1621 tgacggcgcg ctcgggcggc aacgcgttcc tcgcgctggc gctgatcgac gcgggcctgg
     1681 tgtcggccga tgcgccctac gtaggcgtgc ccgcctccct gcgcgacatc ctcgacgcgg
     1741 agctcgcctc gctcgacgac gagacgctcg cgatgctccg cgccgcggcg ctggagccgg
     1801 ggcccctcga cgacgcggtg ctcgccggcg tgctcgaggt gccggtcacg gcagtcggga
     1861 gggcgatccg cgaagcccac cacgcggggg tcctcgccat tgggcgcggc gtcggcgccg
     1921 agagggttcc acgcttccgg cacgccatcc agcgcgaggt cctcgtcgat cagctcgggc
     1981 atggcgagca acggagcctc cacgcccgat acgcggacgt cctcgagtcg gcggatctcg
     2041 acccgtcacg ggcatccgcc gtcgccctcc atcgcgacgg agcgggcgat ggcccgcggg
     2101 cgctcgcggc gcacctcgcg gcgatggatg cggccgagcg agccttcgcg ttcgacgccg
     2161 ccgcaaggca cgggacccgg gccgcggctc tccgtcgcgg gatcggcgtg gcggacccgg
     2221 gcacccccga cgaggccggc ctgctcgggc gggcgtcgta cgcggcgctg ctggcgggcg
     2281 atgcacccgc ggccgccgag ctcgctcgtc gggcgctggc gacgacggag gtcgacgatg
     2341 cccgggccgc cgtcctccac gaccggatcc ggtgggcgct gtggctcgag ggcgatcggg
     2401 cgggctcggc gcgcgagctt gagctcgcgt tcgagcggct gggcgacgcg ccgcccgacg
     2461 cgctccgggc gcacctcctg gccgagcggg cggcgatgcg cacgggcgag gccgacccgc
     2521 gggccgcgct ggacctcgcg caagaggcga tcgggatcgc gcgagccgtc ggggcgcccg
     2581 acatcgaggc gctggggctg gggatcctgg ggcgcgccct cgccacgcac ggccgcgtcg
     2641 acgagggcat cggcagcctt cgagcggccg tggggatcgc ggaccggatc gggaacct
//
LOCUS       DBNHXO010000457         1507 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4905973, whole genome shotgun sequence.
ACCESSION   DBNHXO010000457 DBNHXO010000000
VERSION     DBNHXO010000457.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4905973"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="AC162A_09205"
     CDS             <1..131
                     /locus_tag="AC162A_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIR5011060.1"
                     /translation="KRSMGKGYAGIDNELYSDPKTGMLFADAKDGLAALTAAVKAL"
     gene            141..755
                     /locus_tag="AC162A_09210"
     CDS             141..755
                     /locus_tag="AC162A_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5011061.1"
                     /translation="MPPTPVPGWPRPAKIAFIGSHGIRKTSALLAFAAEAQRAGRSVE
                     LGREVVRDNPLGINEGATGEAQLWVLVTQIRQELELARRADILATDRGVMDNFAYYLR
                     SCGGTDEYAVEPLVRRWSATYDLVVRLLPDVALQADGVRSTNDAFRDEVEAILDRVLP
                     GLVADDRLVTLRASEITSRFDWFAVLQRLAGSIGTTVAAPDLWR"
     gene            complement(774..>1507)
                     /locus_tag="AC162A_09215"
     CDS             complement(774..>1507)
                     /locus_tag="AC162A_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011062.1"
                     /translation="NLRYAQCRPVVAEQLRALVLADAVPRRRVTPSADVRAFRPVVDG
                     LLAEWDDAAADAAFAMNMDLDEPRERRRAAIERVVADLGPFRPDGARPDESFSPAHLA
                     WWLRGERGWVRVSILVTPEPRPLVQQLTVTAVHDPSPALRELAERVLAITAEPETAWP
                     SDLAAGPDLDPIAVRRSLRAAGARFGSMRLGLPTAGDGVTTATFDVDVDDGKAVLAVT
                     LEAETGAVTAVSLSAAEREAPAEAW"
BASE COUNT          205 a          555 c          555 g          192 t
ORIGIN      
        1 tcaagcgctc gatgggcaag ggctacgcgg gcatcgacaa cgagctgtac agcgatccca
       61 agaccgggat gctgttcgcc gacgcgaagg acggcctcgc ggccctcacg gcggcggtca
      121 aggcgctcta gccttcgggc atgccgccga cgcctgttcc tgggtggccg cgtcccgcca
      181 agatcgcgtt catcgggtca cacgggatcc gcaagacctc ggcgctgctc gcgttcgccg
      241 ccgaggcgca gcgggccggg cgatccgtgg agctcggccg cgaggtcgtg cgcgacaacc
      301 cgctgggcat caacgaaggc gcgaccggcg aggcgcagct gtgggtgctc gtcacccaga
      361 tccgccagga gctggagctc gcccgccggg ccgacatcct ggccaccgac cgcggcgtga
      421 tggacaactt cgcctactac ctgcgctcgt gcggcggcac ggacgagtac gcggtcgagc
      481 cgctggtccg ccgctggtcc gcgacctacg acctggtcgt gcgcctgctc cccgacgtcg
      541 ccctccaggc cgacggcgtc cgctcgacca acgacgcctt tcgtgacgag gtcgaggcga
      601 tcctcgaccg ggtgctgccg ggcctcgtcg cggacgatcg tctcgtgacc ctgcgcgcct
      661 cggagatcac cagccggttc gactggttcg cggtgctcca gcgactggcc ggctcgatcg
      721 ggacgacggt ggccgcgccc gacctctggc gctgacgcac cgggccggcg cgcctaccag
      781 gcttcagcgg gcgcctcgcg ctcggcggcc gacagcgaca cggccgtgac ggcgccggtc
      841 tccgcctcga gcgtgacggc cagcacggcc ttgccatcgt cgacgtcgac gtcgaacgtt
      901 gccgtcgtca ccccgtcgcc ggccgtcggc agtcccagcc gcatcgaccc gaagcgagcg
      961 ccggcggcgc gcagggagcg cctgaccgcg atcgggtcga gatcggggcc cgcggcgaga
     1021 tccgacggcc aggcggtctc gggctcggcg gtgatggcga ggacgcgttc ggccagctcc
     1081 cgcagcgccg gcgacgggtc gtggacggcg gtgacggtga gctgctggac gagcggtcgc
     1141 ggctccggcg tcacgaggat cgagacccgg acccagccgc gttcgccgcg gagccaccag
     1201 gcgagatggg caggcgagaa cgactcgtcc ggccgcgcgc catcgggccg gaacgggccc
     1261 aggtcggcga caacgcgctc gatcgccgcg cggcggcgct cgcgcggctc gtcgaggtcc
     1321 atgttcatcg cgaacgctgc gtcggccgcc gcgtcgtccc actcggcaag caggccgtcg
     1381 acgacaggcc ggaaggcccg gacgtccgct gacggcgtga cccggcgccg cggaaccgcg
     1441 tcggccagga ccagggcccg cagctgttcg gcgaccaccg gccggcactg cgcgtagcgg
     1501 aggttgc
//
LOCUS       DBNHXO010000458         3620 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1927797, whole genome shotgun sequence.
ACCESSION   DBNHXO010000458 DBNHXO010000000
VERSION     DBNHXO010000458.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1927797"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="AC162A_09220"
     CDS             complement(<1..487)
                     /locus_tag="AC162A_09220"
                     /inference="COORDINATES: protein motif:HMM:NF015953.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR5011063.1"
                     /translation="MNLTALYTTQLRVLWQWRGGRKALIKRLLITLLVSSIAFLLTAL
                     LLPGITLDRLLDGVIVVIVMALLNALVRPVVLAIVAPRSLILTGISVIVLQILVFLVG
                     ANVVPGVHINGVLTAFVGSFVYAIINTILTAILGIDSGDSFYGLLIQNLLLKRAAPKS
                     DQ"
     gene            complement(636..1124)
                     /locus_tag="AC162A_09225"
     CDS             complement(636..1124)
                     /locus_tag="AC162A_09225"
                     /inference="COORDINATES: protein motif:HMM:NF018590.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HIR5011064.1"
                     /translation="MSRIVVIAFDTEEQAREALATLRGVEKQGQIHFEDTAVVTHKAD
                     GSFDVKNEASSATEAGAVVGGLIGGVVFVIFPIAGIALGALAGAGVGAAMGHGVPGDF
                     VKEVEAKLPAGKSALFLEIQSANAEVAIAALRKYHGEIIQTSLDEETEEALKAALASP
                     AS"
     gene            complement(1121..1510)
                     /locus_tag="AC162A_09230"
     CDS             complement(1121..1510)
                     /locus_tag="AC162A_09230"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5011065.1"
                     /translation="MSTRDDPMTLIGDCAFHGRAAEPMIADIGYTMDPSSQGHGYATE
                     AVGELVRYLIEDRGKHKVCADCDTRNDGSWKLLERLGFRREGEIRGAYRDGEGWASEY
                     LYGMLAEDWRRRATLDANPPNGTGSTP"
     gene            complement(1535..1726)
                     /locus_tag="AC162A_09235"
     CDS             complement(1535..1726)
                     /locus_tag="AC162A_09235"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5011066.1"
                     /translation="MDADFDRLLTDRLVIRRFAVQDAEAFARYRSLPDVARYQSWEAP
                     YSIERARAFVDWMASHQPD"
     gene            1780..3126
                     /locus_tag="AC162A_09240"
     CDS             1780..3126
                     /locus_tag="AC162A_09240"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5011067.1"
                     /translation="MADALPDRPPFVLRARVLTPLAAGGTRYEADGRIEVDAAGRIAA
                     IGSWPGDGAPEGAIDLRPLVVLPGMVDLHVHLPQLPNAGVGAGLDLLTWLRRYIFPLE
                     RAFDEEAAERLAPLAFRAFAAAGTTTAVMYGAVYESSLDAAFRAAEAHGIRAVIGKVM
                     MDRGSYDDTLSSARVLDISLRQSADLCARWHMRDDGRLRYAFTPRFALSCGPDMLRRS
                     ADLANETGAYWQTHVAEDRNEIREVALAFPDAIDYLDVYDQAGGLGPRTILAHAIHLS
                     PREIARVAETDSAIAHCPASNLFLASGAMPLARYQAAGIRVGLGSDVSAGPELSIFAS
                     MRAGGYIQSGLRVLADERGEPGDDTAPLGPLDWLRMGTYEGARALGQQDAIGSLEAGK
                     EADMIAVDPRMVAPVPGIDSDDPAEVMSRFAFRPHTEMVRAAWVRGRRLDGPPGIA"
     gene            3123..>3620
                     /locus_tag="AC162A_09245"
     CDS             3123..>3620
                     /locus_tag="AC162A_09245"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5011068.1"
                     /translation="MTLDADLLITGGKLVDGTGSPRWPGRIAVVGDRLATPPDDVDVA
                     ARRTIDATGLVVAPGFIDLHSHGGLVMLAEPRHEPKVRQGVTTELIGVDGNAYAPFPS
                     REDLLDFAVLNGGLDGRPDIAYDWSTVAEYLARYDGAASVNVAYVVGNSPLRIAAIGW
                     DEIEAD"
BASE COUNT          520 a         1294 c         1255 g          551 t
ORIGIN      
        1 gctggtccga cttcggcgcg gcccgcttga gcagcaggtt ctggatcagc aggccgtaga
       61 acgagtcgcc gctgtcgatg cccaggatcg ccgtcaggat cgtgttgatg atcgcgtaca
      121 cgaacgagcc gacgaaggct gtcaggaccc cgttgatgtg cacgcccgga acgacgttgg
      181 ccccgacgag gaacaccagg atctggagca cgatcacgga gatgccggtc aggatcagcg
      241 acctgggcgc gacgatcgcg agcaccacag gccggacgag cgcgttgagc agcgccatga
      301 cgatgacgac gatgacgccg tcgagcagcc ggtcgagcgt gatgcccggc aggagcagcg
      361 ccgtcagcag gaacgcgatc gaggacacga ggagcgtgat gagcaggcgc ttgatcagcg
      421 ccttgcgtcc cccacgccac tgccacagca cgcgaagctg ggtcgtgtat aacgcggtga
      481 gattcatgac gcccctctac acccggtcag ggtcgctgca ttggcgggcc agtacctgct
      541 gatcttgcga acggtactcg ttccggagca gcgcgacgtg ctccacaacg ggtgatatcg
      601 cgccttgggt cgtgaccggc cgacccggcg tttcgtcagc tggcggggct cgcgagcgcg
      661 gccttgaggg cctcctcggt ctcctcgtcc agcgacgtct ggatgatctc cccgtggtac
      721 ttccgcagcg cggcgatcgc gacctccgcg ttggccgact ggatctcgag gaacagcgcc
      781 gacttgccgg ccggcagctt cgcctcgacc tccttcacga agtcgccggg cacgccgtgg
      841 cccatggcgg ccccgacgcc ggcgcccgcg agggcaccca gcgcgatgcc ggcgatcggg
      901 aagatcacga acacgacgcc gccgatgagg ccgccgacaa cggcccccgc ctccgtggcg
      961 ctgctggcct cgttcttcac gtcgaacgac ccgtcggcct tgtgggtgac gacggcggtg
     1021 tcctcgaagt ggatctgacc ctgcttctcg acgccgcgca gcgtcgcgag cgcctcacgc
     1081 gcctgctcct cggtgtcaaa ggcgatgacg acgatccggc tcatggcgtg ctcccggtcc
     1141 cgttgggtgg attcgcatcc agagtagccc gtcggcgcca gtcctcggcc agcattccgt
     1201 acaggtactc gctcgcccag ccctcgccgt cccggtacgc gccgcggatc tcgccctcgc
     1261 gccggaaccc gagccgctcg agcagcttcc acgacccatc gttgcgggtg tcgcagtcgg
     1321 cgcagacctt gtgcttgccg cggtcctcga tcaggtagcg cacgagctcg cccaccgcct
     1381 cggtcgcgta gccgtgcccc tgggacgacg ggtccatcgt gtacccgatg tcagcgatca
     1441 tcggttcggc cgcgcgaccg tgaaacgcgc agtcgccgat gagggtcatc gggtcgtcgc
     1501 gggttgagat cgcgaactgg taccactcgc cccgctagtc gggttggtgg gaggccatcc
     1561 agtcgacgaa cgcccgcgcc cgctcgatcg aatagggcgc ctcccacgac tgatagcgcg
     1621 cgacgtcggg cagcgagcga tagcgggcga acgcctcggc gtcctgcacg gcgaaccgac
     1681 ggatcacgag ccggtcggtc agcaggcggt cgaagtcggc gtccacggtc gcattgtgcg
     1741 tcgcttgggc tcgtcgtcgc gcatgtagca tccgatctga tggcagacgc ccttcccgac
     1801 cggccgccgt tcgtgctccg tgcgcgtgtc ctgacgccgc ttgccgccgg cggcacgcgg
     1861 tacgaggcgg acgggcggat cgaggtggac gccgccggcc ggatcgccgc catcgggtcg
     1921 tggccggggg acggtgctcc cgagggcgcc atcgacctgc gcccgctggt cgtgctgcct
     1981 ggcatggtcg acctgcacgt ccacctgcca cagctgccga acgcgggcgt cggcgcgggc
     2041 ctggacctcc tcacctggct ccggcggtac atcttcccgc tcgagcgggc gttcgacgag
     2101 gaggccgccg agcgcctggc gccgctcgcg ttccgcgcgt tcgcggcggc cggtacgacg
     2161 accgcggtca tgtacggggc ggtgtacgag tcctccctcg acgccgcgtt ccgggccgcc
     2221 gaggcgcacg ggattcgcgc cgtcatcggc aaggtgatga tggatcgcgg gagctacgac
     2281 gacacgctct cgtccgcccg cgtcctcgac atcagccttc gtcagtcagc cgacctgtgc
     2341 gcgcgctggc acatgcgcga cgacggccgc cttcgctatg cgttcacgcc ccgattcgcg
     2401 ctctcgtgcg ggcctgacat gctccgccgc tccgccgacc tcgccaacga gaccggcgcc
     2461 tattggcaga cgcacgtcgc ggaggatcgc aacgagatcc gcgaggttgc gctggcgttc
     2521 cccgacgcca tcgactacct cgacgtctac gaccaggccg gcgggctcgg cccgcgcacg
     2581 atcctcgccc acgcgatcca cctctccccg cgcgagatcg cgagagtggc cgagaccgac
     2641 tcggccatcg cccattgccc ggcatcgaac ctgttcctcg cctcgggggc aatgccactc
     2701 gcgcgctacc aggcggcggg catccgggtg ggcctcggct ccgacgtgtc cgcgggaccc
     2761 gagctgtcga tcttcgccag catgcgcgcg ggcggctaca tccagagcgg gctgcgggtc
     2821 ctggccgacg agcggggtga gcccggcgac gacacggcac cgctggggcc gctcgactgg
     2881 ttgcggatgg gcacgtatga gggcgcgcgg gccctcggtc agcaggatgc aatcgggtcg
     2941 ctcgaggccg gcaaggaggc cgacatgatc gcggtcgacc cacggatggt ggcgccggtc
     3001 cccgggatcg attcggacga cccggcggag gtcatgtcgc gattcgcatt ccggccgcac
     3061 acggagatgg tgcgcgcggc gtgggtgcgg ggccgccgcc tggacgggcc gccggggatc
     3121 gcgtgacgct cgacgccgat ctgctgatca cgggcggcaa gctcgtcgac gggacgggct
     3181 cgccccgctg gccgggccgc atcgccgtgg tcggggaccg gctcgccacc ccgccggacg
     3241 acgtcgacgt cgccgcccgc cggacgatcg acgcgaccgg actcgtcgtg gcgcccgggt
     3301 tcatcgacct ccacagccac ggcggcctgg tgatgctcgc cgagccccgt cacgagccga
     3361 aggtgcgcca gggtgtcacg acggagctga ttggcgtcga cggcaacgcg tacgccccgt
     3421 ttccgtcgcg cgaggacctg ctcgacttcg cggtcctcaa cggcggcctc gacggccggc
     3481 ccgacatcgc ctacgactgg tcgaccgtcg ccgagtacct cgcccgctac gacggcgcgg
     3541 catcggtcaa cgtcgcctac gtggtcggca actcgccgct gcgcatcgcc gccatcggct
     3601 gggacgagat tgaggccgac
//
LOCUS       DBNHXO010000459         7284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2326254, whole genome shotgun sequence.
ACCESSION   DBNHXO010000459 DBNHXO010000000
VERSION     DBNHXO010000459.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2326254"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="AC162A_09250"
     CDS             <1..1003
                     /locus_tag="AC162A_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011069.1"
                     /translation="VPTDSRSKRRRTLGRLAVLVPLVLLAVVVGVGVLGRASTPPKPT
                     TADQQQVADASPEPSAAASATVPATTPAPRRPPFPVSIDGLTVKSIPTLLATLTDTRP
                     GSVIAVAGYLGVTDPPTDCGDGIDPLGPLCERMALFAETPWTLRGSEGFTGLGPHLHP
                     RVPIGIHLPAAIADTTTAAGGSPMSAVIIGRLSDTPEGNCGPVLANCEVGFDLDAVAW
                     VSGSPFGVQPFIGPQIDAIAREWVLSNQQAAEALAVGPSGIVISTALVRPATLRTLDP
                     KAARIVRRDPPPGLVWYIRGVSRSLFRGATETLVWAVVDDVNLRILAKGVEGGAPTR"
     gene            complement(1008..2417)
                     /locus_tag="AC162A_09255"
     CDS             complement(1008..2417)
                     /locus_tag="AC162A_09255"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR5011070.1"
                     /translation="MSVGEVADDLALRGWVVDPVANREGAGEVLVRDGRLSGAVRWLE
                     GAGADGLSPDGVLITPGFTDLHAHFREPGNEDAETIASGVAAAAHGGFTTVCLMPNTT
                     PAIDDPSVVGRIGSAAERSGSPVRVLVHGAVTAGRAGETLAALGELADAGVVGYSDDG
                     APVRSAAILRNALAYAGSLGLPIVDHPEDPSLTDGAEAAEGYVSTVLGLKGWPGSAEA
                     GSVARSIAILADVVRDVPGARLHLTHISTAASLSQVRAAKAAGLPVTCDVTPHHIGLT
                     DQWIAGSRRWAWESLDAVGRALDPWADGSLVAAPFDTSLRVNPPLRPAEDAAACLAAL
                     LDGTVDAIATDHAPHTEVDKHVEFGWAANGISGIETAIGIVLAAVDAGVMPLARAVAA
                     LTTGPAGVLAGRRTGDGVSTSFGDGDPADLVVIDRGASWTVDADALRSKGKNTPLLGR
                     DLTGVVRLTVAGGRIAFEA"
     gene            complement(2414..3421)
                     /locus_tag="AC162A_09260"
     CDS             complement(2414..3421)
                     /locus_tag="AC162A_09260"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIR5011071.1"
                     /translation="MTAEVDRPPAASAGSQHWSHRHLLDVDALTREDIDLVMRTTDGM
                     HEVLDRPIPKVPALRGTNVTILFYEASTRTRVSFEVAAKNLSADVVNVAASTSSVSKG
                     ESLVDTVRTIEALGADMLVIRHQASGAPYLAAEVFGGSVLNGGDGWHAHPSQALLDLY
                     TLRSRLPGGSVEGRKVVILGDVLHSRVARSNIWTLTAMGADLWLCGPSTLLRGFEAWA
                     ARGAAGRRFTVTSDVSAALRDADVVMALRIQRERMASGLLPSLREYAARYGLTRERLR
                     AASPDVVVMHPGPMNEGVEIAADVAVDPRSVITDQVTNGVAVRMALLYLLAGTRPEAA
                     S"
     gene            complement(3418..3984)
                     /gene="pyrR"
                     /locus_tag="AC162A_09265"
     CDS             complement(3418..3984)
                     /gene="pyrR"
                     /locus_tag="AC162A_09265"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013615161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIR5011072.1"
                     /translation="MSGRRIMADEDVRRATTRISHEIVEKQAGTDGLVLVGIQRRGVP
                     LAHRLAASILEHEGAEIPVGALDITFYRDDLSLVAQQPIVKGTQIPFDLNGRTVVLVD
                     DVLYTGRTIRAAMDALIDFGRPQAIRLAVLVDRGHRELPIRADHVGKNVPTSREEIVR
                     VQLEEIDGEDGVEIERQPLLETVADARG"
     gene            complement(4125..4676)
                     /locus_tag="AC162A_09270"
     CDS             complement(4125..4676)
                     /locus_tag="AC162A_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011073.1"
                     /translation="MRRRTVVAVAALIAAFALAGLPVPAGSRTPSIVRALDEPAFTSL
                     TIPANAAALSAPGGSGAASRSAGSLADTSTFLEPGEAPDGPTARPKVDQPEPRSGSAW
                     KPPKSKMTGFATFYDYGTTAMRLPRGTVVRICGDGGCIQRTVTDYGPQKKSRIVDLYR
                     PDFFKICGCPSWSGTTTVTVSIY"
     gene            complement(4738..6555)
                     /locus_tag="AC162A_09275"
     CDS             complement(4738..6555)
                     /locus_tag="AC162A_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011074.1"
                     /translation="MSPRIRREPFAADHERARELAAQRVDGPVAPDDAGWLNDHLAWC
                     GPCRAVAAEYDEQRLALRALRHDPPTPPRDLWARTAAAIEAEPARRRPTAAGRRRTRL
                     VGFAPLVAALVVAVALGGSFLDGVLPPLGSTTKGDDPEATPIDLVAGEIQVLSHADDG
                     SLELLSRNLDQLCPVAAESCGLSNVLDVTHVASIGSNGELDAIVSPDRGQLVVVESGK
                     GSSSIFVVPLKKHQALNPTPGSGTSTASHQPASDDPGTTATVDPADTASPDTTDEPGS
                     DDPGESPDASDAPPSDTPPSDAAGSPPASSEPTDDPGASDEPVTTPDPTDEPEPEPTP
                     KATEPAPTVEVTPGPDGAIEIARNVVLVGSAAGYSPDGSRFAFSARPAKGSAGPDVFV
                     WRVGDRLAKAVTTDHGSQLAGWIGDRLLVSRVVDGKPRTSILDLDDGSERHVGDGAMW
                     RPTVGPGRELATWWDGTVKQADDGITWVPDRGRLVLANWPDGSDSQVLAEGAVTDWDV
                     AWDETGTKLAVWVSRDDPGKPGKLSLYTVDEATGRPDLARPPLDGAPAYAGFALEPGR
                     LAWSSPAAGGDTSVVVLGWSGNNFGKVTLPTENGATVLR"
     gene            complement(6552..7214)
                     /locus_tag="AC162A_09280"
     CDS             complement(6552..7214)
                     /locus_tag="AC162A_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5011075.1"
                     /translation="MAADPFELQRRTLPVGLTVSTGWDRDEAYTFVEELFAKHQNEIY
                     AYLLRMLRDPDLAADLAQDAFVKAYRAYDSLQKPENARAWLYQIAHRVALDHLRRDRI
                     VRFVPLVGEARNTAPSAEHLVMDANLSGELQRALERIPERQRSALLLAELHDLSGLEL
                     AAALGVSHVAARALLTRARESLRQALAAERKAELDFEAAAEARAAEKHDQDPGRRWGR
                     RS"
BASE COUNT          988 a         2627 c         2701 g          968 t
ORIGIN      
        1 ggtccccacg gacagtcgct cgaagcgccg ccgcacgctc ggccgcctcg cggtcctcgt
       61 gccgctggtg ctgctggcgg tggtcgtagg cgtcggcgtc ctcggccggg cgtccacgcc
      121 gcccaagccg acgaccgcgg accagcaaca ggtcgcggac gccagtccgg agccgtccgc
      181 cgcggcgtcg gcgacggtgc ccgcaacgac gcccgccccg aggcggccgc cgttccccgt
      241 gtccatcgac ggtctcaccg tcaagtcgat cccgacgctc ctcgccacgc tcaccgacac
      301 gcgccccggc agcgtgatcg cggtggccgg ctacctcggg gtcaccgacc cgccaaccga
      361 ttgcggcgat ggcatcgacc cgctcgggcc gctgtgcgag cgaatggcac tgttcgcgga
      421 gaccccgtgg acgctgcggg gcagcgaggg gttcacgggc ctgggcccgc acctccaccc
      481 gcgggtgccg atcggcatcc acctcccggc cgcgatcgcc gacacgacca cggcggcggg
      541 cgggtcgccg atgtccgccg tgatcatcgg gcggctcagc gacactccgg agggcaactg
      601 cgggcccgtg ctcgccaatt gcgaggtcgg cttcgacctc gacgctgtcg cctgggtcag
      661 cgggagcccg ttcggggtcc agccgttcat cgggccccag atcgacgcca tcgcccggga
      721 gtgggtgctg agcaaccagc aggcggccga ggcgctggcg gtcggcccca gcggcatcgt
      781 gatctcgacg gcgctggtcc gccccgcgac gctccggacg ctcgacccga aggcagcccg
      841 gatcgtccgc cgcgacccgc caccgggcct cgtctggtac atccggggcg tgagccgctc
      901 gctcttccga ggagccaccg agaccctcgt ctgggcggtc gtcgacgacg tcaacctgcg
      961 tatcctggcg aagggcgtcg agggcggggc cccaacccgg taggagctca ggcctcgaag
     1021 gcgatccggc cgccggcgac cgtcagccgc actacgccag tcaggtcgcg tcctagtagc
     1081 ggcgtgttct tgcccttcga gcgcagggca tcggcatcga cggtccagct cgcaccgcgg
     1141 tcgatgacca cgagatcggc gggatcgccg tcaccgaacg acgtcgacac cccatcgccc
     1201 gtccgccgcc cggcgaggac cccggccgga cccgtcgtga gtgccgcgac ggcgcgcgcg
     1261 agcggcatca ccccagcgtc gaccgccgcc agcacgatcc cgatcgccgt ctcgatgccg
     1321 ctgatcccgt tggccgccca gccgaactcg acatgcttgt cgacctccgt gtggggcgcg
     1381 tggtcggtgg cgatcgcatc caccgttccg tcgagcagcg cagccagaca cgcggcggcg
     1441 tcctcggcgg ggcgaagcgg cgggttgacg cgcagcgacg tgtcgaacgg ggcggcgacg
     1501 agcgacccgt cggcccacgg gtcgagcgcc cgtccgacgg catccagcga ctcccaggcc
     1561 cagcgccgcg aaccggcgat ccactggtcc gtcaggccga tgtggtgcgg cgtcacgtcg
     1621 caggtgaccg gcaggccggc ggccttggcc gcgcgaacct gggacagcga ggccgccgtc
     1681 gagatgtgcg tgaggtggag ccgcgcgccg ggcacgtcgc gcacgacgtc ggcgaggatc
     1741 gcgatcgagc gcgcgacgga ccccgcctcg gcggaccccg gccagccctt gaggccgagc
     1801 acggtcgaga catacccctc ggcggcctcc gcgccatcgg tgagcgacgg gtcctccggg
     1861 tggtccacga tcgggaggcc cagcgagccg gcataggcga gagcgttgcg caggatcgct
     1921 gccgagcgca cgggcgcccc gtcgtcggag taaccgacga ccccggcgtc ggcgagctcg
     1981 cccagcgcgg ccagcgtctc tcccgcacgg ccggccgtga ccgcgccgtg gacgagcacg
     2041 cgcaccggcg acccgctccg ctccgccgcc gaccctatcc gcccgaccac cgacgggtcg
     2101 tcgatcgccg gcgtcgtgtt gggcatcagg cacacggtcg tgaacccgcc gtgcgccgcg
     2161 gcggccacgc cggacgcgat ggtctccgcg tcttcgttgc cgggctcccg gaagtgcgcg
     2221 tgcaggtcgg tgaagcccgg cgtgatcagc accccatcgg gagagaggcc atccgccccg
     2281 gccccctcga gccagcgcac cgcgccagag agccggccgt cgcgaaccag gacctcgccc
     2341 gccccctcgc gattggcgac cggatcgacg acccagccac gcaacgcgag gtcgtcggcg
     2401 acctcaccga cgctcacgac gcggcctcgg ggcgcgtccc ggccaggagg tacagcagcg
     2461 ccatgcggac ggcgacgccg ttggtgacct ggtccgtgat cacggaccgc ggatcgaccg
     2521 cgacgtcggc cgcgatctcg accccctcgt tcatcggtcc cggatgcatg acgacgacgt
     2581 ccggcgacgc ggcccgcagc cgctcgcggg taagcccgta gcgcgccgcg tactcgcgca
     2641 gcgacggcaa cagccccgag gccatccgct cgcgctggat ccgcagggcc atgacgacgt
     2701 cggcgtcgcg gagcgcggcc gagacgtcgg aggtgaccgt gaaccggcgc ccggcggcgc
     2761 cccgtgcggc ccacgcctcg aacccgcgca gcagcgtgga cgggccgcac agccacaggt
     2821 cggcgcccat cgcggtcagc gtccagatgt tcgagcgggc cacccgcgag tggaggacgt
     2881 cgcccaggat cacgaccttg cgaccctcga ccgagccgcc cggcagccgc gaccggagcg
     2941 tgtacaggtc gagcagcgcc tgcgagggat gcgcatgcca gccgtcgccg ccgttgagca
     3001 ccgacccgcc gaacacctcg gccgcgaggt acggggcccc cgacgcctgg tgccggatca
     3061 cgagcatgtc cgcgcccagc gcctcgatcg tgcggacggt gtcgacgagc gactccccct
     3121 tcgacaccga cgacgtcgag gccgcgacgt tgacgacgtc ggccgagagg ttcttggccg
     3181 cgacctcgaa gctgacgcgg gtccgcgtcg aggcctcgta gaacaggatc gtcacattcg
     3241 tcccgcgcaa ggccggcacc ttggggatcg ggcggtccag gacctcgtgc atgccgtcgg
     3301 tcgtgcgcat gacgaggtcg atgtcctcgc gtgtcagcgc atcgacgtcg agcaggtgac
     3361 ggtgcgacca gtgctgggaa cccgcggacg ccgccggcgg gcggtcgacc tcggcggtca
     3421 tcctcgggcg tccgcgaccg tctcgaggag gggctggcgc tcgatctcga cgccgtcctc
     3481 gccgtcgatc tcctcgagct ggaccctgac gatctcctcg cgcgaggtgg gcacgttctt
     3541 gccgacgtgg tcggcgcgga tcgggagctc gcggtgaccg cgatcgacca gcaccgccag
     3601 ccggatcgcc tgcggacgcc cgaagtcgat cagcgcgtcc atcgcggccc ggatcgtgcg
     3661 ccccgtgtac agcacgtcat cgacgagcac gaccgtccgg ccgttgaggt cgaacgggat
     3721 ctgcgtcccc ttgacgatcg gctgctgggc gacgagcgag aggtcgtcgc gatagaaggt
     3781 gatgtccagt gcgcccacgg ggatctcggc gccctcgtgc tcgaggatcg aggcggccag
     3841 ccgatgggcg aggggcacac cgcggcgctg gatgccgacc aggacgaggc cgtcggtgcc
     3901 cgcctgcttc tcgacgatct cgtgcgagat acgggtcgtc gcccggcgga cgtcctcgtc
     3961 cgccatgatc cggcgaccgc tcatcggcga gcgctgcgtc gacgcacgga gcaggtcctc
     4021 cttcccggcc tcacggggcc attccggcct cacgggaccg gcacggaaat cgagcccagt
     4081 ctagcgcgcc acgggtcgcg ggaccgtcgc ggcggagcga tcgatcagta gatcgagacc
     4141 gtgacggtag tggtgcccga ccacgacggg cagccgcaga tcttgaagaa gtcggggcgg
     4201 tagaggtcga cgatgcgcga cttcttctgc ggtccgtagt cggtcacggt ccgctggatg
     4261 cagccgccgt cgccgcagat ccggacgacc gtgccgcgag gcaggcgcat cgcggtcgtc
     4321 ccgtagtcgt agaaggtcgc gaaccccgtc atcttcgact tcggcggctt ccatgcggat
     4381 cccgaccgcg gttccggctg gtcgaccttc gggcgggccg tgggaccgtc cggggcctcg
     4441 ccgggctcca ggaaggtcga ggtgtccgcg agcgagcccg cggaccgcga ggcagcaccg
     4501 gatccgccgg gggcgctgag ggccgcagcg ttcgcaggga tggtgagtga ggtgaaggct
     4561 ggctcatcca gcgcccgaac gattgagggg gtcctcgatc cagcgggaac cggcaggccg
     4621 gccagggcga acgcggcgat gagggccgcg accgcgacga ccgttctccg tcgcatgacc
     4681 gccggaggtt accccattcg accccctacc gccgaatcgg gcgcgccccg tgagtcgtca
     4741 gcggaggacg gtcgcgccgt tctccgtggg cagcgtgacc ttgccaaagt tgttgccgga
     4801 ccaccccagg acgaccacgc tggtgtcgcc gccagcggcg gggctggacc atgcgagccg
     4861 gcccggctcg agggcgaacc cggcgtaggc cggagcccca tcgagcggcg ggcgggcgag
     4921 gtccgggcgg cccgtcgcct catcgaccgt gtacaggctg agcttccccg gcttgcccgg
     4981 gtcgtcgcgc gacacccaca cggcgagctt ggtgcccgtc tcgtcccagg cgacgtccca
     5041 gtccgtgacc gcaccctcgg cgaggacctg gctgtcgctg ccgtcgggcc agttggccag
     5101 gaccaggcga ccgcgatcag gcacccacgt gatgccatcg tcggcctgct tgacggtgcc
     5161 atcccaccag gtcgccagct cgcgtccggg gccaaccgtc gggcgccaca tcgcgccgtc
     5221 gcccacgtgc cgctcggagc cgtcgtcgag atcgaggatg gatgtccgcg gcttgccgtc
     5281 gacgacgcgg ctcaccagca ggcggtcgcc gatccagcct gcgagctgcg agccgtgatc
     5341 ggtggtgacg gccttggcca ggcgatcgcc cacgcgccac acgaacacgt ccggcccggc
     5401 cgagcccttg gcggggcggg cggagaaggc gaaccgcgac ccatcgggcg agtagccggc
     5461 agcggatccg acgaggacga cgttgcgggc gatctcgatc gcgccgtccg gcccgggtgt
     5521 gacctccacg gtcggcgccg gctcggtcgc cttgggggtc ggctccggct ccggctcgtc
     5581 ggtcgggtcg ggcgtggtga cgggctcgtc gctcgctccg ggatcgtcgg tcggctcgga
     5641 cgaggcgggc ggcgagcccg cggcgtcgct gggcggggtg tcgctgggcg gcgcatcgga
     5701 cgcgtcgggc gactcgcccg ggtcgtccga gcccggctcg tcggtggtgt cgggcgacgc
     5761 cgtgtccgcg gggtcgaccg tcgcggtggt gccgggatcg tccgaggccg gttggtgcga
     5821 ggcggtcgac gtgcccgagc cgggggtcgg gttcaacgcc tgatgcttct tgagcggcac
     5881 cacgaagatg gagctggacc ccttgccact ctcgacgacc acgagctggc cgcggtccgg
     5941 cgagacgatc gcatcgagct cgccgttcga gccgatgctc gccacgtggg tgacgtcgag
     6001 cacgttcgag agcccgcagc tctcggcggc gaccggacac agctggtcga ggttgcggct
     6061 caggagctcg agcgacccgt catcggcatg gctcaggacc tggatctcgc cggcgacgag
     6121 gtcgatcggg gtggcttcgg gatcgtcgcc cttggtggtg ctgccgagcg gcggcaggac
     6181 gccatcgagg aacgagccac cgagcgcgac cgcgaccacg agcgccgcga cgagcggggc
     6241 gaatccaacc agacgggtcc ggcggcggcc ggccgcggtt gggcgacgtc gggcgggctc
     6301 ggcctcgatc gccgccgccg tccgcgccca caggtcgcgc ggcggggtcg gcgggtcgtg
     6361 gcgcagggcg cgcagggcga gtcgctgctc gtcgtactcg gccgccacgg cgcggcacgg
     6421 gccgcaccag gcgaggtggt cgttcagcca gccggcatcg tccggggcga ccggtccatc
     6481 gacccgctgc gccgcgagct cgcgggcgcg ctcgtggtcg gcggcgaacg gctcgcgccg
     6541 gatcctcgga ctcacgatcg gcgaccccac cggcggccgg ggtcctgatc gtgcttctcg
     6601 gcggcgcgcg cctcggcggc ggcctcgaag tccagctcgg ccttgcgttc ggcggccagc
     6661 gcctggcgga ggctctcgcg ggcgcgggtc aggagggcgc gggcggcgac gtgggagacg
     6721 ccgagggccg cggcgagctc gagcccggac aggtcatgga gctcggcgag gaggagcgcg
     6781 gaccgctggc gctccgggat ccgctcgagg gcccgctgga gctcgcccga caggttggcg
     6841 tccatcacca ggtgctcggc ggacggggcg gtgttgcgcg cttcgccgac cagcggcacg
     6901 aaccggacga tgcggtcgcg gcggaggtga tcgagggcga cgcggtgggc gatctggtac
     6961 agccacgcgc gggcgttctc gggcttctgg agcgagtcgt aggcgcgata cgccttgacg
     7021 aacgcgtcct gggcgaggtc cgcggcgagg tcggggtcgc gcagcatgcg cagcaggtag
     7081 gcgtagatct cgttctggtg cttcgcgaac agctcttcga cgaacgtgta cgcctcgtcg
     7141 cgatcccagc ccgtgctcac ggtgaggccc acgggaagcg ttcggcgctg gagctcgaag
     7201 gggtcggctg ccacggctgc tccgatcggg tcgatcggat gggcacaccg atcgggagcg
     7261 ttgcactcca gccgcctttg acgg
//
LOCUS       DBNHXO010000460         3784 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5502588, whole genome shotgun sequence.
ACCESSION   DBNHXO010000460 DBNHXO010000000
VERSION     DBNHXO010000460.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5502588"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="AC162A_09285"
     CDS             complement(<1..758)
                     /locus_tag="AC162A_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR5011076.1"
                     /translation="MGGEGARALLVATLSTIIVFGLIGYVVVNSTGWPRFQETFLNSE
                     IFWESLPKLISKFWVNVRLFLTAEVLILVFGLVLAVLRSLSGPALFPVRLLATVYVDV
                     FRAIPGLLIIFILGLGVPALRIPGVPDDEFFWAVIALTLVYSAYVSEVYRSGIDSVHP
                     SQDAAARSLGLSRWQGLRFVVLPQAVRRVIPPLLNDFIGLQKDTVLVASIGVVEVFRQ
                     TQALQAATFNFTPFLATALIFLVVTIPLARFTDYL"
     gene            complement(834..1805)
                     /locus_tag="AC162A_09290"
     CDS             complement(834..1805)
                     /locus_tag="AC162A_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5011077.1"
                     /translation="MRIIKGTTALALLAVVAAACTGTGGGATSAPTAAATPAATPAAS
                     VEAPSAPAAESPSAAPTADACAPETLALKTAGKLTIGADNPAYPPYFEESDPQADPWD
                     FGNPTNGKGFESAMGFAIADEMGFGKDAVSWVVTPFNNAIQPGPKDFDIYLTQVSYSP
                     ERAQAVDLSDGYYDLTQSLVVPEGSKFADAKSIADLKDMVLGAQVGTTSLKTITDVIA
                     PNTEPKIYDTNDIAVKQLQNGKLDGLVVDLPTASYVAFVQLETGTILGQFANSAAGEH
                     FSAVLDKDSPLTACVNAAIGRLAAAGTLDALATQWLPDLVSVPVFQP"
     gene            complement(1864..2682)
                     /locus_tag="AC162A_09295"
     CDS             complement(1864..2682)
                     /locus_tag="AC162A_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM hydrolase/SAM-dependent halogenase family
                     protein"
                     /protein_id="HIR5011078.1"
                     /translation="MSRPIVSLLTDFGARDVSAAICRAVVLGICPDAEVIDLSHEIRK
                     YSIRDGALALWSALPYLPVGVHVAVVDPGVGTARRPLAIRAGRGDVLIGPDNGLLVSA
                     AERLGGVGEVRELAHPAYRLPVVTSTFHGRDIFAPAAGHLAAGAAFDTLGPAIDPATL
                     VGSPLPVASPIEGGGLATEVIYEDTFGNLKLSALVDDVRAVLGDPTGGTFELRTGDGR
                     TLHLGWGRTFGELAPGEPLLYEDSYGRTCIAVNQGSAVSALGLRDGTRLELRRA"
     gene            2951..>3784
                     /locus_tag="AC162A_09300"
     CDS             2951..>3784
                     /locus_tag="AC162A_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011079.1"
                     /translation="MRTIELRILAMLLVVLWFVTCGLVLIGYRPGGPVDVLVGLAAIG
                     PVLIAVAAVAWPPVARGDRAFAGIAWLGLFAVLLLVPSIAGVITQLVGRGPQTLLPSF
                     EAAYPWGVALIATGLYAGLGIARRRLGGRMIRRRRLLVGAGIALLMVLAAGSVFGAVA
                     IVNELALGNRPAIASRFGPTDPDAEVPPCTGALAAGSTARLDLRLDASVDDRRTGQVV
                     IAGIRNGRDVRWNGFAATRLLVGQVGAARVGERAWERQPGTAWTETSLGSLENRDLDV
                     PL"
BASE COUNT          559 a         1271 c         1401 g          553 t
ORIGIN      
        1 aggtagtcgg tgaaccgggc gagcgggatc gtgaccacca ggaagatcag cgccgtcgcg
       61 aggaagggcg tgaagttgaa cgtggcggcc tggagggcct gggtctggcg gaacacctcg
      121 acgacgccga tcgacgcgac gaggaccgtg tccttctgca ggccgatgaa atcgttgagc
      181 agcggcggga tgacgcgccg taccgcctgg ggcaggacga cgaaccggag cccctgccag
      241 cgcgacaggc ccaaggaccg tgccgccgca tcctggctgg gatggacgga gtcgatgccg
      301 gaccggtaga cctccgacac gtacgccgag tacacgaggg tcagcgcgat gacggcccag
      361 aagaactcgt catcgggcac gcccgggata cgcagcgccg ggacgccgag gcccaggatg
      421 aagatgatca gcagccccgg gatagcccgg aacacgtcca cgtagaccgt cgccagcagc
      481 cggaccggga acagcgccgg acccgacagg ctgcgaagca ccgccaggac gaggccgaac
      541 acgaggatca gcacctcggc ggtcaggaac agccggacat tgacccagaa cttgctgatc
      601 agcttgggca gcgactccca gaagatctcg gagttgagga acgtctcctg gaagcgcggc
      661 cagcccgtcg agttgacgac gacgtagccg atcagcccga agacgatgat cgtcgagagc
      721 gtcgcgacga gcagcgccct cgcgccctcg ccgcccaacc cgaaacgcgg cccccgcggt
      781 cggcggggcg gagcctccag ccctgcctgc tgggccgcgt ccgggcccac gggtcagggc
      841 tggaagacgg ggacgctcac gaggtccggc agccactgcg tggccagggc gtcgagcgtc
      901 ccggcagcgg ccaggcgccc gatcgcggcg ttcacgcacg cggtgagcgg gctgtccttg
      961 tccagcaccg cgctgaagtg ctcgcctgcc gcggagttcg cgaactggcc gaggatggtg
     1021 ccggtttcga gctggacaaa ggcgacgtac gaggcggtcg gcaggtcgac gacgagcccg
     1081 tcgagcttgc cgttctggag ctgcttcacg gcgatgtcgt tggtgtcgta gatcttcggc
     1141 tcggtgttcg gcgcgatgac gtccgtgatg gtcttgaggc tggtcgtgcc gacctgggcg
     1201 ccgagcacca tgtccttgag gtccgcgatc gacttcgcgt cggcgaactt gctgccctcc
     1261 ggaacgacca gcgactgggt caggtcgtag tagccgtcgc tgaggtcgac ggcctgcgcc
     1321 cgctcggggc tatacgagac ctgggtcagg tagatgtcga agtccttggg ccccggctgg
     1381 atcgcgttgt tgaacggggt gacgacccat gagaccgcat ccttcccgaa gcccatctca
     1441 tcggcgatcg cgaagcccat ggcgctctcg aagcccttgc cgttggtcgg gttgccgaag
     1501 tcccaagggt cggcttgcgg gtcggactcc tcgaagtacg gcggataggc cgggttgtcg
     1561 gcgccgatgg tgagcttgcc cgcggtcttg agcgcgagtg tctccggcgc gcaggcgtcg
     1621 gcggtcggcg cggccgaagg cgactcggcc gccggcgcgg acggggcctc gacgctcgcc
     1681 gctggtgttg cggcgggggt cgctgctgct gtcggggccg aggtcgcgcc accgccggtg
     1741 cctgtgcagg ccgcggcgac gacggcaaga agtgcgaggg cggtcgtgcc cttgatgatg
     1801 cgcatcggtc gttggtctcc tcaagaagtg cccggcggca gtgtagcggt cgcggccaaa
     1861 gggtcaggcc cggcgcagct ccagccgcgt cccgtcgcgc aggccgagtg cgctcacggc
     1921 cgacccctga ttcacggcga tgcaggtgcg cccgtatgaa tcctcgtaca ggagcggctc
     1981 gcccggcgcg agctccccga acgtccgacc ccagccgagg tggagcgtgc gaccgtcgcc
     2041 cgtgcgcagc tcgaatgttc cgcccgtcgg atcgcccagg acggcacgca cgtcgtcgac
     2101 cagcgccgag agcttgaggt tcccgaacgt gtcctcgtag atcacctcgg tcgcgaggcc
     2161 gccgccctcg atcggactgg ccaccggcag cggcgatccg acgagcgtcg ccgggtcgat
     2221 cgccgggcca agcgtgtcga acgcggcgcc agccgcaagg tggcccgcgg cgggagcgaa
     2281 gatgtcacgc ccgtggaacg tgctcgtgac caccgggagg cggtaggcgg gatgtgcgag
     2341 ctcccggacc tcgccgacgc cacccagacg ctccgccgcc gacacgagca agccgttgtc
     2401 cgggccgatg aggacgtcgc cccttccagc gcggatggcc agcggccgcc gcgccgtccc
     2461 cacgcccggg tcgacgaccg ccacgtgaac tccgacgggc aggtagggca gcgcggacca
     2521 cagcgcgagc gccccatcgc ggatcgagta cttgcggatc tcgtgcgaga ggtcgatgac
     2581 ctcggcgtcg gggcagatgc cgagcaccac cgcccggcag atcgccgcgg agacgtcgcg
     2641 tgcgccgaag tcggtcagca gggacacgat gggccggctc atcgccggga tggtagcggc
     2701 cgcaggcgag gagacacctc ccgggaacac cgaagcggcg ccgggaggag gcacggcgcc
     2761 gcttcggtag agggttttcg gacgcttcgg gaggagggag aaccgacagc gccctggatt
     2821 gttcggttgc gggcactaca cgcgggtgca gtgcgctgac tatggggttc gatggtcccc
     2881 gcgctgatga cccgtgagtc ccagtcgacc gggtactttg cgtcacgctc ggtagactcc
     2941 gcgggtgcgc gtgcggacga tcgaactgcg gatcctggcc atgctgctcg tggtgctgtg
     3001 gttcgtcacc tgcggcctgg tcctgatcgg ctatcggccg ggcggcccgg tggacgtcct
     3061 ggtgggcctc gcggcgatcg ggcccgtgct catcgcggtc gccgcggtcg cgtggccgcc
     3121 ggtcgcccgc ggcgaccgcg cgttcgccgg gatcgcctgg ctcgggttgt tcgccgtgct
     3181 gctcctggtg ccttcgatcg cgggcgtcat cacccagctg gtcggccgtg gcccgcagac
     3241 actgttgccg tcgttcgaag ccgcctatcc gtggggcgtc gccctgattg cgaccggcct
     3301 gtacgcgggc ctgggcatcg cccggcggcg cctcggcggc cgcatgatcc ggcgtcggag
     3361 gctcctcgtc ggagcgggga tcgccttgct catggtgctc gccgccgggt ccgtgttcgg
     3421 cgcggtggcc atcgtcaacg agctcgccct gggcaatcga ccggcgatcg catcccgctt
     3481 cggcccgacc gatcccgacg ccgaggtccc gccctgcacc ggcgcgctcg cggcgggttc
     3541 gacggcgcgc ctcgacctgc gcctcgacgc atcggtcgac gaccggcgga cggggcaggt
     3601 cgtgatcgcg ggcatccgca acgggcgtga cgttcgctgg aacgggttcg ccgcgaccag
     3661 gctgctggtg ggccaggtcg gcgccgcacg ggtcggcgag cgagcctggg aacggcagcc
     3721 cgggaccgcc tggacggaga cctcgctcgg gtccctcgag aaccgcgatc tcgacgtgcc
     3781 gctg
//
LOCUS       DBNHXO010000461         2384 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4907584, whole genome shotgun sequence.
ACCESSION   DBNHXO010000461 DBNHXO010000000
VERSION     DBNHXO010000461.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4907584"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="AC162A_09305"
     CDS             complement(<1..449)
                     /locus_tag="AC162A_09305"
                     /inference="COORDINATES: protein motif:HMM:NF014269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR5011080.1"
                     /translation="MQIPLFPLHTVLSPGLALPLHVFEDRYRLMVRRCVDESVPFGVV
                     LIHEGSELAPRGGRPQELAIASVGTFAEIREASRYADGRWELLTVGAGRFVIETVKTG
                     VEPYLVADVEPLDDAVGDAETADALVRRVSRRFVDYLRLLQPRDGEDA"
     gene            complement(576..956)
                     /locus_tag="AC162A_09310"
     CDS             complement(576..956)
                     /locus_tag="AC162A_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR5011081.1"
                     /translation="MIDAQPTVVSLSDAAAVKLRELTKDEPNPQIGLRVYVYSGGCSG
                     YRYGMMLEDAPTSDDNVLSANGVRVYIDNNSVPLIQGSEIDYVDTLMGAGFTVNNPNA
                     VSGCGCGSSFRTADDAGQARGCSH"
     gene            complement(1069..2298)
                     /locus_tag="AC162A_09315"
     CDS             complement(1069..2298)
                     /locus_tag="AC162A_09315"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR5011082.1"
                     /translation="MVRDLQRPLREAWIVDAVRTPIGRYGGALAAVRPDDLAAVVIAA
                     IVERTGVDPAAIEDVVFGCANGAGEDNRNVARMALLLAGLPVEVAGQTVNRLCGSGLQ
                     AVNTAAHAIGAGDGEVFVAGGVESMTRAPYVMLKAEGAWDRGPRTVEDTTLGWRFVNP
                     RMHEAYPPISLGETAECVADQWTVSRERQDAFALESQRRAIAAIEGGRFDGQLVPVNV
                     SGRKGAVTVVDRDEHPRADTSAEALAALKPAFKAGGTVTAGNSSGINDGAAALLLVEA
                     DRARAMGLRPMARVVATAVAGVHPDVMGIGPVPAVRKVLGRAGLEAGDLDVVEINEAF
                     ASQSVACMDELGLDPERVNPNGGAIALGHPLGASGARLVTMVVHELDRTGGRYGLATM
                     CIGVGQGIATIVERIEP"
BASE COUNT          338 a          906 c          816 g          324 t
ORIGIN      
        1 gcgtcctcgc cgtcgcgcgg ctggagcagg cggaggtagt cgacgaaccg acgcgagacc
       61 cggcggacca gggcgtcggc ggtctcggcg tcacccaccg cgtcgtcgag cggctcgacg
      121 tcggccacga ggtagggctc gacgcccgtc ttcacggtct cgataacgaa cctgccggcc
      181 ccgaccgtca gcagctccca gcgcccatcg gcgtaccggc tcgcctcgcg gatctcggcg
      241 aatgtgccga cgctcgcgat cgccagctcc tgcggccggc cgccgcgtgg ggccagctcg
      301 ctgccctcgt ggatcaggac gaccccgaat ggaaccgact cgtcgacgca gcgccggacc
      361 atcagccggt agcggtcctc gaacacgtgc agcggcagcg ccaggcccgg cgacagcacg
      421 gtatgcaggg ggaagagcgg gatctgcatg atcgttcgcg cagggtacgc cgtcggcggg
      481 atcggcggcg cgggcaacgg gacatcaaaa cgccgccggg cgataccgac ggcgtcctgc
      541 gcgactcgag gcgggcgcca ccggcgcccg gagggtcagt gcgagcagcc ccgcgcctga
      601 ccggcgtcgt cagcggtccg gaagctggag ccgcagccgc agcccgagac agcgttcggg
      661 ttgttgaccg tgaagcccgc ccccatgagc gtgtcgacgt agtcgatctc gctgccctgg
      721 atgagcggca cgctgttgtt gtcgatgtag acgcggacgc cgtttgcgct caggacgttg
      781 tcgtccgagg tcggcgcgtc ctcaagcatc atcccgtagc ggtagccgga gcagccgccg
      841 gagtacacgt agacgcgcag cccgatctgg gggttcggct cgtccttcgt cagctcgcgc
      901 agcttgacgg ctgcggcgtc gctgagagag acgacggtcg gctgggcgtc gatcatgacg
      961 tgagatctcc tcgggtcgct acgccccgtg gcccgcacgg cccgggaaca ctccgatcgt
     1021 acgcaccgca acgggtttcg tccagcaccc cgatcgtccc agcctcgcct agggctcgat
     1081 acgctccacg atcgtggcga tcccctggcc cacgccgatg cacattgtcg cgagcccgta
     1141 gcgacccccg gtccggtcga gctcgtggac caccatcgtg acgaggcgcg cgcccgacgc
     1201 cccgagcgga tgcccgagcg cgatcgcgcc gccgttgggg ttcacgcgct cgggatcgag
     1261 gcctagctcg tccatgcacg cgaccgactg gctggcgaac gcctcgttga tctcgacgac
     1321 gtcgaggtca ccggcctcga ggccggcccg ccccaggacc ttgcgcacgg ccggcaccgg
     1381 cccgatgccc atcacgtcgg ggtggacgcc cgcgacggcc gtcgcgacga cacgcgccat
     1441 cggccgcaga cccatcgcgc gggcgcggtc cgcctcgacc aacagcagcg ccgccgcccc
     1501 gtcgttgatg cccgagctgt tgcccgcggt gaccgttccg cccgccttga acgcgggctt
     1561 gagcgccgcc agcgcctcgg ccgaggtgtc ggcgcgggga tgctcgtcgc ggtcgaccac
     1621 ggtgaccgcg cccttgcggc ccgacacgtt gaccgggacc aactggccgt cgaaccgacc
     1681 gccttcgatc gccgcgatcg cgcgtcgctg actctccagc gcgaaggcgt cctggcgctc
     1741 gcgcgagacg gtccactggt cggcgacgca ctccgcggtc tcgccgagcg agatcggcgg
     1801 gtaggcctcg tgcattcgcg ggttcacgaa tcgccagccg agggtcgtgt cctcgaccgt
     1861 ccgcgggccg cgatcccagg cgccctcggc cttgagcatc acgtacggcg cccgggtcat
     1921 cgactcgacg ccaccggcca cgaacacctc gccatcgccc gccccgatcg cgtgggccgc
     1981 cgtgttgacc gcctgcagcc ccgaaccgca caggcggttc accgtctggc ccgcgacctc
     2041 caccggcagc ccggcgagca gcagcgccat gcgcgccacg ttgcggttgt cctcgcccgc
     2101 gccgttggcg cagccgaaca cgacgtcctc gatcgccgcc gggtcgacgc ccgtccgctc
     2161 gacgatcgcc gcgatcacga ccgccgcgag gtcatcgggc cggaccgccg ccagcgcgcc
     2221 gccgtagcgg ccgatcgggg tccggaccgc gtcgacgatc cacgcctcgc gcaggggccg
     2281 ctgcagatca cgcaccatgg accgattgtg gcatgggaac cgagcgcacg atcccggcgt
     2341 ccgggcgcca tgtcccaacg cgcccgactc ggccggaccg ctcc
//
LOCUS       DBNHXO010000462         1979 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2329377, whole genome shotgun sequence.
ACCESSION   DBNHXO010000462 DBNHXO010000000
VERSION     DBNHXO010000462.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2329377"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1919
                     /locus_tag="AC162A_09320"
     CDS             <1..1919
                     /locus_tag="AC162A_09320"
                     /inference="COORDINATES: protein motif:HMM:NF027510.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HIR5011083.1"
                     /translation="ALNATAPVTLTVTTDDQGNSPAPALNDVDNLTLNVTAVNDAPTA
                     AAPAAYAATEQTLLNLAGTGLTVGDADALGANETVTLSVLSGVLNATAGATGVVVAGS
                     GTSTLTLVGTIAQLNDLLAGNLGATLDYFNASDAPPAADTLTLSIDDGGNTGAGGALN
                     ASADSTLNIAAVNDAPLNTVPGPQAVNEDTPLMFSVAGGNAIAVADADAATLRVTLTA
                     TNGVLTLASLAGLGFTAGDGAADATMTFTGTAAAINFALDGLSFLPALNYNGPAAVAI
                     TTEDLGQTGAGGPLQDADVVNVAVNAVNDAPVIGAQAFAVSDGQTVAIGTANLSATDA
                     DDAATSLVFTVGAATNGHFELVAAPGVAITSFTQAQILAGEVRFVHDGSNVAPTAAVY
                     VNDAATGVGAYALNITFTASAGPTVTPPAGGGGGSGGGTPVVVPPPPLLPVTKAETGS
                     AGERIGDTFFRAPTTPPADGGDDGGAVEVAAPVVAAAPGGAQVQKVLGAEAIQPVGRA
                     EGESVEVKPLSSEVEVEPVRAEMQILPMRRDAFDNPNDEERQTIEVIMSSVKVTGLAF
                     SVGAVWWAVRAAGLVASLLASSPAWRHVDPLPVLGRDDEEEEGEWDEASEEEKDRKDD
                     EHRAAWVLEEREAS"
BASE COUNT          300 a          776 c          683 g          220 t
ORIGIN      
        1 ccgcgctcaa cgccaccgcg ccggtcaccc tcaccgtcac caccgacgac cagggcaact
       61 cccccgcgcc agccctcaac gacgtcgaca acctcaccct caacgtcacc gcagtcaacg
      121 acgcgcccac tgccgcggcc cccgccgctt atgccgccac ggagcagacg ctcctcaacc
      181 tcgccggcac cggcctcacc gtgggcgacg cggacgcgct cggcgcgaac gagacggtca
      241 cgctctcggt cctctccggc gtgctgaacg cgaccgccgg cgcgaccggc gtcgtcgtgg
      301 cgggctcggg cacgagcacg ctcacgctgg tcgggacgat cgcccagctc aacgatctgc
      361 tcgccggcaa cctcggcgcg acgctcgact acttcaacgc atcggacgcc ccaccggccg
      421 ccgacacgct cacgctgtcg atcgacgacg gcggcaacac cggtgccggc ggcgcgctca
      481 acgcctccgc cgactcgacc ctcaacatcg ccgcggtcaa cgacgcgccg ctgaacacgg
      541 tgcccgggcc gcaggcggtg aacgaggaca cgcccctcat gttctcggtc gccggcggca
      601 acgcgatcgc ggtcgccgac gcggacgcgg ccaccctccg ggtcacgctc accgccacca
      661 acggcgtcct gacgctcgcc tcgctcgccg ggctcggctt caccgccggc gacggcgccg
      721 ccgacgccac gatgaccttc accggcaccg ctgcagcgat caacttcgcg ctcgacggac
      781 tgtccttcct gccggcgctg aactacaacg gcccggccgc cgtcgcgatc accaccgagg
      841 acctcggcca gaccggcgcc ggcgggccgc tccaggacgc ggacgtcgtg aacgtcgccg
      901 tcaacgcggt gaacgacgcg ccggtgatcg gggcgcaggc gttcgcggtc agcgacggcc
      961 agacggtggc gatcggcacc gcgaacctga gcgcaaccga cgccgacgac gcggccacga
     1021 gcctcgtctt cacggtcggg gcggcgacga acgggcactt cgagctcgtg gccgcgccgg
     1081 gcgtggcgat cacgagcttc acccaggcgc agatcctcgc cggcgaggtc cgcttcgtcc
     1141 acgacggctc gaacgtcgcg cccaccgccg cggtctacgt caacgacgcc gcaaccggcg
     1201 tcggggcgta cgccctcaac atcaccttca ccgccagcgc cgggccgacg gtcacaccgc
     1261 ccgccggcgg cggcggcggg agcggcggcg gcacgccggt cgtggtgccc ccgccgccgc
     1321 tcctgccggt cacgaaagcc gagaccggga gcgccggcga gcgcatcggg gacacgttct
     1381 tccgcgcgcc gacgaccccg ccggccgacg gcggcgacga cggcggcgcg gtcgaggtcg
     1441 cggcgccggt cgtcgcggcc gcgccgggcg gcgcccaggt gcaaaaggtg ctcggggccg
     1501 aggccatcca gccggtgggc cgcgccgagg gcgagtcggt ggaagtgaag ccgctcagct
     1561 cggaggtcga ggtcgagccg gtccgcgccg agatgcagat cctgccgatg cgccgcgacg
     1621 cgttcgacaa cccgaacgac gaggagcgcc agacgatcga ggtgatcatg agctcggtca
     1681 aggtcaccgg cctcgcgttc tcggtcggcg cggtctggtg ggccgtgcgc gccgccggcc
     1741 tcgtggcgag cctgctcgct tcctcgcccg cctggcgcca cgtcgatccg ctgccggtgc
     1801 tcggccgcga cgatgaggag gaagaaggcg agtgggacga ggcgtccgag gaagagaagg
     1861 accgcaagga cgacgagcac cgcgcggcct gggtgctcga ggagcgggaa gcgtcgtaac
     1921 gcccgtacgg gtgtcccagc gtcgctcccc cgccggaagc cgacgcggac acgtcaggc
//
LOCUS       DBNHXO010000463         1544 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3519025, whole genome shotgun sequence.
ACCESSION   DBNHXO010000463 DBNHXO010000000
VERSION     DBNHXO010000463.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3519025"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="AC162A_09325"
     CDS             complement(<1..512)
                     /locus_tag="AC162A_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011084.1"
                     /translation="MGDLFDDFMRELERRRAQAEGRQPPSDKDGGPTDDPDDRDPDDD
                     GPPSADDQDADRARNDDGADDPEPDTQERPGAEPRAAGPTPITSRRRRRGRSSPPPPS
                     GGGRPPGGPTDGARGTTAGDVARRVGVAALILAIGAILLLLVAGINLWTDAIWYRSVG
                     YDSVFWTRLGV"
     gene            complement(585..1043)
                     /locus_tag="AC162A_09330"
     CDS             complement(585..1043)
                     /locus_tag="AC162A_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011085.1"
                     /translation="MSERLPHPGPSFLREQEVRIGDRRLEAGMAKPVDGPDDWWLAVL
                     WVTDADGVVSFRDVAPAAGPPPEPPLARLGPSLAGGLSGLIREEDGRLAIRLTPLVPP
                     EDDTRPWRCPLVIRAAFRWEPALAATLRSNQIADRVLGAFGRSVEGLARP"
     gene            complement(1069..>1544)
                     /locus_tag="AC162A_09335"
     CDS             complement(1069..>1544)
                     /locus_tag="AC162A_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011086.1"
                     /translation="TSLDPLAIIQARAEVARPRNRIMSFITGDTTGNVDPAANASSFA
                     PPPAEVRREMRRLEIEAEVADLEAESVALKTDVVVEMGISLSALAGADADLAAAEAPP
                     DEAAQVEAEQARLAAAAAAPSVSSAPAAPTPPAPVVPSAPPAPAAAPDAEETPGA"
BASE COUNT          185 a          553 c          612 g          194 t
ORIGIN      
        1 acgcccagcc gggtccagaa cacgctgtcg tagccgacgc tgcgatacca gatcgcgtcg
       61 gtccacaggt tgatcccggc gacgagcagc agcaggatcg cgccgatcgc gagaatcagg
      121 gccgcgacgc cgacccggcg ggcgacgtcg cctgccgtgg tccctcgggc gccgtcggtc
      181 gggcctcccg gcggtctgcc gccgcccgaa ggggggggtg ggctgctgcg accgcgtcgg
      241 cggcggcgcg atgtgatcgg ggtgggtccc gcggcgcgcg gctcggcgcc cgggcgctcc
      301 tgcgtgtcgg gctcgggatc gtccgcacca tcgtcgttcc gggcgcgatc cgcgtcctgg
      361 tcgtcggcgc tcgggggacc gtcgtcgtcc gggtcgcgat cgtccggatc gtcggtcggg
      421 ccgccgtcct tgtcgctcgg cggctggcgg ccttccgcct gcgcgcgccg gcgctcgagc
      481 tcgcgcatga agtcgtcgaa caggtcgccc atcgcgcgga gtctagggcg gattgcccga
      541 acccggatcg gggggcgcgg gatgcggccc ccatccggct cggatcaagg gcgggccagc
      601 ccctcgacgg atcgcccgaa cgcgccgagg acgcggtccg caatctgatt cgagcgcagg
      661 gtcgcggcga gcgccggctc ccagcggaag gccgcgcgga tcaccagcgg gcagcgccag
      721 ggacgcgtgt cgtcctcggg cgggacgagc ggggtgagcc ggatcgcgag ccgcccgtcc
      781 tcttcccgga tgagccctga cagcccgcct gccagcgatg gacccaggcg cgcgagcggc
      841 ggctcgggcg gcggaccggc cgcgggcgcc acgtcccgga agctcaccac gccgtcggca
      901 tcggtcaccc acaggaccgc cagccaccag tcatcgggtc catcgacggg cttggccatg
      961 ccggcctcga gccggcgatc gccaatgcgg acctcctgct cgcgcaggaa cgaagggccg
     1021 ggatgcggga gtcgctcgga cacgccccga ggataccggg gcggcgggtc aggcccccgg
     1081 tgtctcctcg gcgtcgggtg ccgcggcggg ggcgggcggc gcgctgggaa cgacgggggc
     1141 cggcggtgtc ggagcggccg gggcggagga gaccgaaggc gccgccgccg cggccgcgag
     1201 cctcgcctgc tcggcctcga cctgggcggc ctcgtcgggc ggggcttcgg ctgccgcgag
     1261 gtcggcgtcc gcgccggcca gcgccgagag cgaaatgccc atctcgacga cgacatcggt
     1321 cttgagggcg accgactcgg cctccagatc ggcaacctcg gcctcgatct cgagccggcg
     1381 catctcgcgc cggacttcgg ccgggggcgg cgcgaacgat gacgcgttgg cggccgggtc
     1441 cacgttgccg gtggtgtcgc cggtgatgaa cgacatgatc cggttccgcg gccgggcgac
     1501 ctcggcgcgg gcctggatga tcgccaacgg gtcgagcgag gtca
//
LOCUS       DBNHXO010000464         1785 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2925367, whole genome shotgun sequence.
ACCESSION   DBNHXO010000464 DBNHXO010000000
VERSION     DBNHXO010000464.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2925367"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1167
                     /locus_tag="AC162A_09340"
     CDS             109..1167
                     /locus_tag="AC162A_09340"
                     /inference="COORDINATES: protein motif:HMM:NF021597.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HIR5011087.1"
                     /translation="MRRLGIILPAAALGLLLTAAPALACGGLIGPNGAVNLLRTTTFA
                     GYHDGDEHYVTAFEFAGGGGAFGSITPLPGIPSSVVKGGDWTLQRLVRETNPQPAFLA
                     EDSVRASAGAIELMKVKIDALDVTVLKGGADDIGAWAKDHGFRLPPDAPEVLDFYASR
                     SPIFLAAAFDADAAAERGQQVGDGTPVHVTIPTDNPWVPLRILALGKTGEERVEADVY
                     LLTDEAPALLPAPTGDNGIRLDYSASASGQLLDDLRSDRGMEWIPSSAWLTKVGVDSS
                     AAGLSYDLAIDASGANAPSRVDAGLDLPGIGGGAETVVDSGRGIVAGRLLAALMVSLA
                     GVAGILALTRYQPGRRSR"
     gene            1178..1603
                     /locus_tag="AC162A_09345"
     CDS             1178..1603
                     /locus_tag="AC162A_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HIR5011088.1"
                     /translation="MSPARVITSLGLGVAALLGAAAMALAVAREPEPIVVDISIRYSH
                     FVPAAIAVPAGRPVTFVLDNGDPIDHEWIVGDAAVHLRHRTGTEPSHGARPTEVSIDA
                     NATRSTTVTFTEPGTLTYVCHLPGHEAYGMTGTVIVTAH"
BASE COUNT          242 a          663 c          626 g          254 t
ORIGIN      
        1 ccgcgatcga tcgagcccgg tgcgtgcccg gcgggaaccg catccgccgg gtcggcgtcc
       61 tgacgccacg gcggacacct cccgtccgcc cgctccggag atcgaccgat gcgacgactc
      121 ggaatcatcc tcccggccgc ggcgctgggc ctccttctca cggccgcccc cgcgctcgcg
      181 tgcggcggcc ttatcggccc caacggcgcc gtcaacctcc tgcggacaac cacgttcgcg
      241 gggtaccacg acggcgacga gcactacgtg acggcgtttg agttcgcggg cggcggcggc
      301 gcgttcggct cgatcacgcc gctgcccggg atcccgtcga gcgtcgtcaa gggcggcgac
      361 tggacgctcc agcgactggt ccgcgagacc aacccgcagc cggccttcct ggccgaggat
      421 tcggtccggg ccagcgccgg cgcgatcgag ctcatgaagg tcaagatcga cgccctggac
      481 gtgacggtgc tcaagggcgg cgcggacgac atcggggcct gggccaagga tcacggcttc
      541 cggctcccgc ccgacgcgcc cgaggtgctc gacttctacg cgagccgtag cccgatcttt
      601 ctcgctgccg cgttcgacgc cgacgcggcc gccgagcgcg gccagcaggt cggcgacggc
      661 acccccgtgc acgtcacgat ccccaccgac aacccgtggg tccccctgcg catcctcgcg
      721 ctgggcaaga ccggcgagga gcgcgtcgag gccgacgtgt acctgctcac cgacgaagcc
      781 cccgcgctgc tcccggcgcc aaccggcgac aacggcatcc gactcgacta cagcgcttcg
      841 gcgagcggcc agctcctcga cgacctgcgc agcgaccgtg ggatggagtg gatcccgtcc
      901 tcggcctggc tgaccaaggt cggcgtcgac tcgtcggccg ccgggctgtc ctacgacctg
      961 gcgatcgatg cgagcggcgc gaacgcaccc tcacgcgtcg acgcgggtct cgacctgccg
     1021 ggcatcggcg gtggagcgga gacggtggtc gacagcgggc gcggcatcgt ggcgggccgg
     1081 ctgctggccg cgctgatggt ctcgctggcc ggggtcgcgg ggatcctcgc gctgacccgc
     1141 taccagcccg gccgccggtc tcgctgaggc gatcggcgtg tcgcccgcac gggtcatcac
     1201 ctcgctcggt ctgggcgtcg ccgcgctgct cggcgcggcg gcgatggccc tcgcggtcgc
     1261 ccgcgagccg gagccgatcg tcgtcgacat ctcgatccgc tattcgcact tcgtgccggc
     1321 ggcgatcgcc gtcccggccg gacggccggt gacgttcgtc ctcgacaatg gcgatccgat
     1381 cgaccacgag tggatcgtcg gcgacgcggc ggtccacctc cgtcatcgca cggggacgga
     1441 gccgtcgcat ggcgcccggc cgaccgaggt cagcatcgat gccaacgcga cgcgctcgac
     1501 cacggtcacg ttcacggagc cggggacgct gacctacgtc tgccacctgc ccggccacga
     1561 ggcgtacggc atgaccggga cggtgatcgt caccgctcac tagctcccat caacgagcga
     1621 acgccccgcc gatggcgggg cgttcgtgct tgtgcgaggg gctgcgggtc agaacccgaa
     1681 ggcgcccggt ggcatctcgg tcacgagcag cgcgttcacg atcggcttgt gggcgcgcgg
     1741 tgccgtgaag ttgatgttga gcctgccgtc ggtgacctcc acgag
//
LOCUS       DBNHXO010000465         3594 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_544614, whole genome shotgun sequence.
ACCESSION   DBNHXO010000465 DBNHXO010000000
VERSION     DBNHXO010000465.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_544614"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="AC162A_09350"
     CDS             <1..476
                     /locus_tag="AC162A_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5011089.1"
                     /translation="AGPFVHGFTYSHQPVAATVALEVLRILESESLVEASAAKGERLL
                     ALLHERLDASPVVGDIRGRGLLVGVELVRDRETREPYARAARLAEAVVRTARERGLLL
                     YSGTGNANGVDGDTLLLGPPFVVTDGELIRIADGLAEALEAAVAAVAAVEAATRA"
     gene            516..1898
                     /locus_tag="AC162A_09355"
     CDS             516..1898
                     /locus_tag="AC162A_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5011090.1"
                     /translation="MTTQEEPAYAVEPGPIGGPVDPVRARRRRRLVAAVGVIVVAAVG
                     LAVAVSVVGRLGQPPTPAGRVAFVDPAGALAIVDAQGGNRVVHAPPGTTFAFPAWSPD
                     GSRVAAVSNGGDGAAIEVFDAAAGTAAPADRTVLFKSDTSAPFYLYWAPDGSAVGFLT
                     QEDSSIALRSAPADGSANPTTLREGAPMYWAWEDPGRMLVHTGADTDAFLGAVGADGS
                     IVSTVDALPGVFRSPSVSADGRYEAYAVAGRGGPAAVVVAASDGSTSFDVPVFGPAAV
                     GFAPSGPQLAFIGAREEDREVPLPLGPLRVIDAGTGAVRQFPVDDVVTFFWSPDGRTI
                     ATIGVADPENSGPAAGTARRAAVRDDDRATRPTVDAPGVDVSLVFVDVASGTGTRPRI
                     VPLSPLFVNQVMPYFDQYALSHRVWAPDGSAILLPLIGDDGEASLAMLPADGSAPRTL
                     APGSMGFWSP"
     gene            complement(1916..2479)
                     /locus_tag="AC162A_09360"
     CDS             complement(1916..2479)
                     /locus_tag="AC162A_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011091.1"
                     /translation="MSEQQHQPPLTGEVYDSGGVKIYGWEWNDIGGPRGRSRLPWFGI
                     FLVVFGALLLLRQFFPALDTAGSLLFLAVGVAFLVSWLINRGLGSLYLGAIITALAAP
                     DLLAAANVVSGAGVGTLCLGIAFLFIALVRGVSGAGWGWQLALGAILSAIGATSLASP
                     GFSDLVWPVTLLVLGVIVLARAMRPAR"
     gene            complement(2476..>3594)
                     /locus_tag="AC162A_09365"
     CDS             complement(2476..>3594)
                     /locus_tag="AC162A_09365"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR5011092.1"
                     /translation="EAAIAIQSGFEAGTTAPIIALVDVPGEPTTDANVRALVAYGDAL
                     GGVGGVERVESPFTGIPNPATGQPLSADELVALYAQPREAWPAPLAGLWDRYVHESTV
                     RFDAISPLQPSSPEGTAVVAAARGIDPGAGFTAMIGGQAATGYDFLASQSDRIPFAVA
                     VVLIAMFFVLFLLFGSVVLPIKAILMTLLSISASFGAMVWIFQEGNLSGPLGFTSPGF
                     TIAGNPIIMFSVLFGLSMDYEVLLLSRVQEAYRRSGDNRAAVAEGLSKTAGVITGAAA
                     IMVTVFAAFALADTITIKSIGVGMAIAVFVDATIVRILLVPATMRLLGKWNWWAPGPL
                     ARFADTLGFSHVEDEATIPTGSAGGRGTLSGGGVTEAT"
BASE COUNT          498 a         1296 c         1334 g          466 t
ORIGIN      
        1 gggccggccc gttcgtccac ggcttcacct actcgcacca gccggtcgcc gcgacggtcg
       61 cgctcgaggt gctccggatc ctcgagtcgg agtcgctggt cgaggcatcg gccgccaagg
      121 gcgagcggct gctcgcgctg ctccacgagc gattggacgc cagcccggtc gtgggcgaca
      181 tccgcgggcg cgggctgctg gtgggcgtgg agctcgtgcg ggaccgggaa acgcgcgagc
      241 catacgcgcg cgcggcgcgg ctggccgagg cggtcgtacg gacggcgcgc gagcgcgggc
      301 tgctgctcta ctccggcacg ggcaacgcga acggcgtcga cggcgacacg ctgctgctgg
      361 gtccgccgtt cgtcgtcacc gacggcgagc tgatcaggat cgccgatggc ctggccgagg
      421 ccctcgaggc cgccgtggcc gcggtggcag ccgtggaggc cgccacccga gcgtgaggtg
      481 atccgggcgg ggttacacgc cgtatcgtca cgtacatgac gacgcaggag gagccggcct
      541 acgccgtgga gcccgggccc atcggcgggc cggtcgatcc cgtccgcgcg cgccggcgcc
      601 ggcggctcgt cgccgcagtc ggggtcatcg tggtcgcggc cgtcggcctg gcggtcgccg
      661 tctccgtcgt cgggcgcctc gggcagccgc ccacgcccgc aggacgcgtc gcgttcgtcg
      721 accccgccgg ggccctcgcg atcgtcgacg cgcagggcgg caaccgtgtc gtgcacgcgc
      781 cgccgggcac gaccttcgcg ttcccggcct ggtcgcccga cgggtcgagg gtggcggcgg
      841 tcagcaacgg tggggacggc gccgcgatcg aggtgttcga cgcggctgcg ggcacggctg
      901 cgcccgcgga caggactgtc ctgttcaaga gcgacacgag cgctccgttc tacctgtact
      961 gggcccccga tggctcggcg gtgggcttcc tcacccagga ggactcgagc atcgcgctcc
     1021 gctcggcgcc cgcggacggc agcgccaacc ccacgacgct ccgcgaaggc gcgccgatgt
     1081 actgggcctg ggaagacccg ggccggatgc tcgtccacac gggggcggac accgacgcgt
     1141 tcctgggtgc cgtcggcgcc gacggcagca tcgtgtccac ggtcgacgcc cttccgggcg
     1201 tgttccgctc cccgtcggtc tcggccgacg gccgctacga ggcgtacgcc gtcgccggcc
     1261 ggggcggccc cgccgcggtc gtggtggcgg cgagcgacgg atcgacgagc ttcgacgtcc
     1321 cggtgttcgg gccggccgcg gtgggcttcg cgccgtccgg gccacagctc gcgttcatcg
     1381 gggcgcgcga ggaggaccgc gaggtgccgc tgccgctcgg cccgctgcgg gtgatcgatg
     1441 ccggcaccgg cgccgtccgg cagttcccgg tcgacgacgt cgtgacgttc ttctggtcac
     1501 ccgacggccg gacgatcgcg acgatcgggg tggcggaccc ggagaactcg ggccccgcgg
     1561 ccgggaccgc gcgccgggcc gccgtgcggg acgacgatcg ggcgacccgg ccaaccgtgg
     1621 acgcgccggg cgtggacgtg tcgctcgtgt tcgtcgacgt cgcctctgga acagggacgc
     1681 ggccacggat cgtcccgctc tcgccactgt tcgtgaacca ggtcatgccg tacttcgacc
     1741 agtacgcgct cagccaccgg gtgtgggcgc ccgacggcag cgcgatcctg ctgccgctga
     1801 tcggcgacga cggcgaggcg tccctggcga tgctcccggc cgacgggtcg gcgccgcgca
     1861 cgctggcgcc gggctcgatg gggttctgga gcccctgagc gttgctcgcg cgtcgctagc
     1921 gggcgggcct catcgcccgc gcgagcacga tcacgcccag caccagcagc gtgaccggcc
     1981 acacgaggtc gctgaatccc gggctcgcga ggctcgtggc gccgatcgcg gacaggatcg
     2041 cgcccagcgc gagctgccag ccccaaccgg ccccgctgac cccgcgcacc agggcgatga
     2101 acaggaacgc gatgcccagg cacagcgtgc cgacgccggc cccggacacg acgttcgcgg
     2161 ccgccagcag gtccggcgcg gcgagcgcgg tgatgatcgc gccgaggtag agcgacccga
     2221 gcccacggtt gatgagccac gagaccagga aggcgacgcc gaccgcgagg aacagcagcg
     2281 agccggcggt gtcgagcgcg gggaagaact ggcgcaggag cagcagcgcc ccgaacacga
     2341 cgaggaagat cccgaaccac ggcagccggc tgcgtccccg cggcccaccg atgtcattcc
     2401 attcccaacc gtagatcttg accccgccgg agtcatagac ctcgccggtc agcggtggct
     2461 ggtgctgttg ctcgctcatg tcgcctccgt cacgcctcct cccgagagcg taccccggcc
     2521 gccggcggag ccggtgggga tggtcgcctc gtcctcgacg tggctgaagc ccagcgtgtc
     2581 ggcgaaccgc gccagcgggc cgggcgccca ccagttccac ttgccgagca gccgcatggt
     2641 cgccgggacg agcaggatgc gcacgatcgt cgcgtccacg aagactgcga tcgccatgcc
     2701 cacgccgatg ctcttgatcg taatggtgtc ggccagcgcg aaggcggcga acacggtgac
     2761 catgatcgcg gcggccccgg tgatcacccc cgccgtcttc gacagtccct cggcgaccgc
     2821 ggcccggttg tcgccgctgc ggcggtaggc ctcctggacc cgcgacagca gcaggacctc
     2881 gtagtccatc gacagcccga acagcacgct gaacatgatg atcgggttgc cggcgatcgt
     2941 gaacccgggc gacgtgaacc cgagcgggcc ggacaggttg ccctcctgga agatccagac
     3001 catcgcgccg aacgaggcgc tgatcgagag cagcgtcatc aggatcgcct tgatcggcag
     3061 cacgaccgag ccgaacagca ggaacaggac gaagaacatc gcgatcagga cgaccgccac
     3121 cgcgaacggg atccggtcgc tctgcgaggc gaggaagtcg tagccggtgg ccgcctggcc
     3181 gccgatcatc gccgtgaatc cggcccctgg atcgatccca cgcgccgcgg caacgaccgc
     3241 cgtgccctcg ggcgacgagg gctggagcgg gctgatggcg tcgaagcgga ccgttgactc
     3301 gtggacgtag cggtcccaca ggcctgccag cggagccggc cacgcctcgc ggggctgggc
     3361 gtagagcgcg acgagctcgt cggcgctgag cggctggccg gtcgctgggt tcgggatgcc
     3421 ggtgaacggg ctctccaccc gctcgacgcc gccgacgccg cccagggcat cgccgtacgc
     3481 gaccagcgcc cgcacgttgg cgtcggtggt tggctcgccc gggacgtcga cgagggcgat
     3541 gatcggcgcc gtcgtgccgg cctcgaagcc ggactggatg gcgatcgcgg cctc
//
LOCUS       DBNHXO010000466         1600 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4513862, whole genome shotgun sequence.
ACCESSION   DBNHXO010000466 DBNHXO010000000
VERSION     DBNHXO010000466.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4513862"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..437)
                     /locus_tag="AC162A_09370"
     CDS             complement(27..437)
                     /locus_tag="AC162A_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HIR5011093.1"
                     /translation="MADKNLTCSDCGMEFAFTEREQAFYAEKGFSEPRRCPSCRASRK
                     AARSGGDSGYGGGGGSYSSGGGSYDSGYGGGGGYSAGGYGSGGGYGSRSSSGPREMFT
                     ATCSSCGKEARVPFRPTNGKPVYCSDCFRTMRGG"
     gene            complement(648..>1600)
                     /locus_tag="AC162A_09375"
     CDS             complement(648..>1600)
                     /locus_tag="AC162A_09375"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HIR5011094.1"
                     /translation="NASRTLLLDIRTLDWDEDLLRLMEVPRSLLPDVRPSSAPWGGTE
                     PAIFGRPIPIAGVAGDQQAAMFGQACFAPGEAKNTYGTGAFLLANVGEGPVASGHGLL
                     STVLWQLGEGGGAAYALEGSVFIAGAAVQWLRDGLRAVSSAEEVEELMRGVKDTGGVY
                     LVPAFVGLGAPYWDPRARGLLIGMTRGTGLPEIARATIESIAYQVSDVVEAMAADRGG
                     PLTELRVDGGAARNDDLLRFQADILGIPVERPRVTETTAWGAASLAGLAVGFWGSLDE
                     LAAIRAVDRRFEPAMAEDRRRTLLDGWHRAVERSKGWATD"
BASE COUNT          230 a          609 c          538 g          223 t
ORIGIN      
        1 gcgacccggt ccgggcgagg tgctggtcag ccgccgcgca tcgtgcggaa gcagtccgag
       61 caatagaccg gcttgccatt cgtgggccgg aacggaacgc gggcttcctt gccgcagctc
      121 gagcaggtcg cagtgaacat ctcacggggc ccgctgctgc tgcggctgcc gtagccgccg
      181 ccgcttccat agccaccggc ggaatagccg ccgccaccgc cgtacccgga gtcatagctg
      241 ccgccgccgc tcgaatacga gccaccgccg ccgccatacc cgctgtcacc acccgagcgc
      301 gctgccttgc ggctggcgcg gcacgagggg caccggcggg gctccgagaa tcccttctcg
      361 gcgtagaagg cctgttcgcg ctccgtgaat gcgaactcca ttccgcagtc ggaacaggtc
      421 aggttcttgt cggccacgtg ccgatctcct ccgttagtcg ccgcctcttg cccgggtcct
      481 cgagtgtttg cgccggagga gaccaagtgt cgaacgtgcg aacgtgtctg tgcaggcgcg
      541 gcgttccgcg ccacgcgcag tctagggtta atccccgaac gtggcgatac cctattttcg
      601 ggcgccgcga acgggacgtt cggctggcgc gggccgtcac cggggcgcta gtccgtggcc
      661 cagcccttcg atcgctcgac cgcccgatgc cagccgtcga ggagcgtgcg ccggcggtcc
      721 tcggccatcg ccggttcgaa gcgacgatcg acggcgcgga tcgccgccaa ctcgtcgagt
      781 gacccccaga atccaaccgc cagtccggcc agcgacgcgg cgccccaggc cgtcgtctcc
      841 gtgacccgcg gccgctccac cgggatgccc aggatgtccg cctggaaccg cagcaggtcg
      901 tcgttgcggg ccgccccgcc gtccacgcgc agctcggtga gcggcccacc gcggtcggcc
      961 gccatcgcct ccaccacgtc gctgacctgg taggcgatcg actcgatggt cgcgcgcgcg
     1021 atctcgggca ggccggtgcc gcgggtcatg ccgatcagga ggccgcgcgc ccgcgggtcc
     1081 cagtagggtg caccgagacc gacgaacgcc ggcacgaggt agacgcctcc cgtgtccttg
     1141 acgccgcgca tgagctcctc gacctcctcg gcggacgaca cggcgcgcag cccgtcccgg
     1201 agccactgga cggcggcgcc ggcgatgaac acggacccct cgagcgcgta ggcggctccc
     1261 ccaccctcgc ccagctgcca caacaccgtc gacagcagcc cgtgcccgga cgcgaccggc
     1321 ccctcgccga cgttcgcgag caggaacgcc ccggtcccgt acgtgttctt cgcctcgccc
     1381 ggcgcaaagc acgcctggcc gaacatcgcc gcctgctggt ctccggccac gccggcgatc
     1441 gggatcggcc ggccgaagat cgcgggctcg gtgccgcccc agggcgcgga cgacgggcgg
     1501 acgtccggga gcagcgatcg cgggacctcc atcaagcgca gcaggtcctc gtcccagtcg
     1561 agggtccgga tgtccaacag cagcgtgcgc gatgcgttcg
//
LOCUS       DBNHXO010000467         9377 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_148223, whole genome shotgun sequence.
ACCESSION   DBNHXO010000467 DBNHXO010000000
VERSION     DBNHXO010000467.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9377)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9377
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_148223"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="AC162A_09380"
     CDS             <1..1004
                     /locus_tag="AC162A_09380"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF014801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HIR5011095.1"
                     /translation="AARSMALMPDLIGYWLSGVIEAEETNASTTGLFDPGRRAWDADL
                     IEVLGLPAFVFGPTAEPGWPLGSIRAAVAADAGLPEGVLVTHVGSHDTASAVVAVPMA
                     AERAAYVACGTWGLVGVELDAPIRDEQSRLANFTNELGVDGRIRFLRNVMGLWLLQES
                     IRTWERGGVAAPLTSLLAAASGHPTGGPTFDPNDASLLAPGDMPARISALIAAAGGSP
                     PAGPAQLVRCILDSLSAAFAATVRDAVRVSGREVDVVHVVGGGSQNALLCQLTADAVG
                     LPVIAGPVEATAIGSILVQARAHGMITGELEDLRALVRSSSELHRFEPRAAHAPRGY"
     gene            1009..1743
                     /locus_tag="AC162A_09385"
     CDS             1009..1743
                     /locus_tag="AC162A_09385"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR5011096.1"
                     /translation="MRVALFITCFNDMLFPDVGRATVRLLRRLGQEVDFPEGQTCCGQ
                     MHFNTGYRADCIPLVRRFAEVFAGYDAVVTPSPSCAGMVRHQHGPIAASTGDAALADA
                     VGTVVPRVYELTEFLVDVLGVTDVGASFRETVTLHPTCHSVRALGIGDRPQRLLGAVD
                     GLTLVDLPDADQCCGFGGTFAAKNADTSLAMGTDKIAAVAATGASVLTAADTSCLMHL
                     GGMLSRAGSPVRVMHLAEILAAEPVA"
     gene            1740..3251
                     /locus_tag="AC162A_09390"
     CDS             1740..3251
                     /locus_tag="AC162A_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HIR5011097.1"
                     /translation="MTAPPSEPARLPATPTFAGTPPFPAAARAALADGQLRTNLAAAT
                     KTIRTKRAKAVGERADWEALRLAGAAIKDEVLGDLPALLEQLEAKVTEAGGTVHWARD
                     AAEANAIVARLVHAAGADEVIKVKSMATQEIELNEALARDGIAAWETDLAELIVQLGG
                     DLPSHILVPAIHRNRREIREIFEREMARAGRPAPADLTDDPRALAEAARLHLREKFLR
                     AKVAISGANFAVAETGTVMVVESEGNGRMCLTLPETLITVMGIEKLVPRWQDLEVFLQ
                     LLPRSSTAERMNPYTSMWTGVTPGDGPQAFHLILLDNGRTDTLVDPVGRQALRCIKCS
                     ACLNVCPVYERTGGKAYGSPYPGPIGAILTPQLRGLNVHPVDAQTASLPYASSLCGAC
                     FEVCPVRIEIPEILVHLRATVVEQHGWRGRVSEEAVLMAAAGWTFRGSSRLGAAERAG
                     GLGGRLLGRGGWIRRAPGPGLLRAWLRGRDLAAPAPESFRRWWRRVGRTEVEP"
     gene            3248..3874
                     /locus_tag="AC162A_09395"
     CDS             3248..3874
                     /locus_tag="AC162A_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutC/YkgG family protein"
                     /protein_id="HIR5011098.1"
                     /translation="MTEARDAVLDRIRATLAAAPAAPSPVPRDYETALAGETDLVELF
                     AERVADYRATIHRSHADGLPATIARILAGWDARTLVVPDAIPAAWLSGATAVVTLRDT
                     PPLTKAQLDATDAVITDAAVGIAETGTIVLDARPSQGRRALSLLPDYHLCVVHVERIA
                     GTVPEALARLDAGRPLTWISGPSATSDIELQRVEGVHGPRVLDVVIVG"
     gene            complement(3871..4362)
                     /locus_tag="AC162A_09400"
     CDS             complement(3871..4362)
                     /locus_tag="AC162A_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5011099.1"
                     /translation="MTEQSSVRAADPKSRIRRILLATDLAPASGDAATWALDLAAAHG
                     AQLLIVSVIDPDELMSDGTGVPSGRAMRWDQIRDDRQEAARRLVARSRTAGVEAAFMV
                     WTGHPGESIVAAASAESADIIVVGSHGRSRIGRMVAGSVSDHVVRHASCPVLVVRASG
                     ATT"
     gene            complement(4412..5170)
                     /locus_tag="AC162A_09405"
     CDS             complement(4412..5170)
                     /locus_tag="AC162A_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HIR5011100.1"
                     /translation="MGALRRMVVPIGILAAALLIVLFNPWLEIEEGTALRQVLTVAFW
                     VTFVVLLVALFEDRNDPEATEVEIEGPRFARFLFSNTRAGLFWLPIRLFLGFAWVEAG
                     WHKLTGTGWVDGGSALAGFWANAAKVPEEGRPPISYEWYRDFVNFLIAGHHETWFAWL
                     ITVGEIAVGLGLLFGALTGFAAFFGALMNMSFLLAGSASTNPVLFTMAIGIILAWRVA
                     GWYGLDRYLLPALGAPWRKGRVFGGPAITSTAPG"
     gene            5364..6587
                     /locus_tag="AC162A_09410"
     CDS             5364..6587
                     /locus_tag="AC162A_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR5011101.1"
                     /translation="MIRGRAHLAALTVLAVIAGACGSEAPTQAVSSTAPTPVAVASPG
                     PSDPTDAGGTTAADGSCVARTLDAMTEAQRIGQLFMIQLPKDQLDAKHRAAIEDDHFG
                     SVWFGRSTAGVEALRAVSNSVQRLATPAATNGVRFLIAANQEGGLIQGLSGPGFATIP
                     SAVDQGRMTPAALTAAAVTWAHELRRAGVNVNFAPTADVVPPGGDATNAPIGQLDREY
                     GHHPRVVASHVAAFIGGMRAGGIATTAKHFPGLGRVEGNTDHTADVVDTVTTPDDAYL
                     RPFRRAIDDAVPFVMVSLATYERIDASAIAAFSPTIVTGLLRTTLGFRGVIMSDSLTA
                     EAVAAIPPADRAIRFIASGGDLIVLGRLATAISMAKGLASLAATSPAFATTIDAAARR
                     VLRAKAAAGLVDCGS"
     gene            complement(6659..6958)
                     /locus_tag="AC162A_09415"
     CDS             complement(6659..6958)
                     /locus_tag="AC162A_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR5011102.1"
                     /translation="MEVKLMNLIAWIVLGAIAGYLAGFLVRGDEGLGVIGHIVLGIVG
                     AIVGGFLASLLFNVKDPLQGAFDLSTIVTSVIGAVIVVVVVSLVTGTRRVGRGPV"
     gene            7169..7876
                     /locus_tag="AC162A_09420"
     CDS             7169..7876
                     /locus_tag="AC162A_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5011103.1"
                     /translation="MKNQPLVLVADDEPPITRLVALALSADFRVVTATGGEEALQIAD
                     QARPDIVLLDVLMPDVDGIEVMRRLREWRPVPVILMTASGSTAQRTRGLDLGADDYVA
                     KPFHPDELAARVRAVLRRASGVAAGSVPVRVGDIEIDLERRHVTQGGRAVSLSRPEWL
                     LLQALATNAGRVVLHGELLTEVWGPEFRDDVQYLRAWISRIRRRLGAQPGEAGPIVTF
                     QGIGYLLDVEQPPAART"
     gene            7985..8347
                     /locus_tag="AC162A_09425"
     CDS             7985..8347
                     /locus_tag="AC162A_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011104.1"
                     /translation="MTTASTTSAARRAPDEARERESVAAARSSLADTVDAVRSTASSV
                     GDRLPEVIETMKSSAADGARSMQAWSASRQRLAVAFSLGLGMGLTLAGAPRLAIASAL
                     VPVIVVAATIVERNEGAS"
     gene            8347..8820
                     /locus_tag="AC162A_09430"
     CDS             8347..8820
                     /locus_tag="AC162A_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011105.1"
                     /translation="MLTRTIRVNGTTIDPAEAIRSTGLDTMPERVDAAINAAGESLRD
                     AGDSLRATVHQLSAPPSRTRMPSTRGWLAGFAFLVAAIALATWLFRRMSAAPMALSAD
                     DLDALDLEDLERAKGEGMGTAVGTAIASDPLHVPSDNDLMGVMAEPAAIGEHNGR"
     gene            8825..9355
                     /locus_tag="AC162A_09435"
     CDS             8825..9355
                     /locus_tag="AC162A_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011106.1"
                     /translation="MRSTRAGPAKAVETWQMPVQLAAAGAIIVVAAEDERFGGALRRA
                     TELATKRGEPLLLYDWDAPSFLAEPLPTWWSSEGSRDQLPDRLDPEQLRTAGRGALAA
                     QVDRARDSGVEAFGWLPSDHGPGPLADYAGAQGASIVVVPRDLTELHGLDALINGTAR
                     PADALVERASAAVVIV"
BASE COUNT         1298 a         3453 c         3352 g         1274 t
ORIGIN      
        1 acgcggcccg gtcgatggcc ctgatgcccg acctgatcgg ctactggctg tcgggcgtga
       61 tcgaggccga ggagacgaac gcgtcgacga ccggcctgtt cgacccgggc cgccgggcgt
      121 gggacgctga cctgatcgag gtgcttggcc tgccggcgtt cgtgttcggg ccaaccgccg
      181 agcccggctg gccgctcggg tcgatccgcg ccgcggtcgc cgcggatgcg gggctgccgg
      241 agggcgtcct ggtgacgcac gtcgggtcgc acgacacggc ctcggcggtc gtggccgtcc
      301 ccatggcagc tgagcgagcc gcctacgtgg cctgcgggac gtgggggctg gtcggcgtcg
      361 agctcgacgc gccgatccgc gacgagcaga gccgcctcgc gaacttcacc aacgagctgg
      421 gcgtggacgg ccggatccgc ttcctgcgca acgtcatggg cctgtggctc ctccaggagt
      481 cgatccggac ctgggagcgg ggcggcgtgg ccgcgccctt gacatcgctg ctggcggccg
      541 cgtccggcca cccgacgggc ggcccgacgt tcgaccccaa cgacgcctcg ctgctggcgc
      601 cgggcgacat gccggcccgg atctccgcgc tgatcgcggc cgcgggcggg tcgccgccgg
      661 ccgggccggc gcagctcgtg cggtgcatcc tcgacagcct ctccgcggcg ttcgccgcga
      721 cggtccgcga cgccgtccgc gtctccgggc gcgaggtcga cgtcgtgcac gtcgtcggcg
      781 gtgggagcca gaacgccctg ctctgccagc tcacggcgga cgccgtgggg ctgccggtca
      841 tcgccggtcc ggtcgaggcg acggcgatcg ggagcatcct cgtccaggca cgggcgcacg
      901 ggatgatcac cggtgagctc gaagatctcc gcgcgctcgt ccgcagctcg tccgagctcc
      961 accggttcga gccccgtgca gcccacgcgc cgcgcggata ctgaggcgat gcgcgtcgcc
     1021 ctgttcatca cctgcttcaa cgacatgctg ttccccgacg ttgggcgggc gacggtccgg
     1081 ctcctgcggc gcctcggcca ggaggtcgac ttccccgagg gccagacgtg ctgcggccag
     1141 atgcacttca acaccgggta ccgtgcggac tgcatccccc tggttcggcg gttcgccgag
     1201 gtgttcgccg ggtacgacgc ggtggtcacg ccctcgccgt cgtgtgccgg gatggtccgc
     1261 caccagcacg gcccgatcgc ggcgtccacc ggcgacgcgg ctctcgccga tgccgtcggc
     1321 accgtggttc cgcgggtcta cgagctcacc gagttcctcg tcgacgtgct gggcgtgacg
     1381 gacgtcgggg cgtcgttccg cgagaccgtg accctgcatc cgacctgcca ctcggtccgc
     1441 gccctcggca tcggcgaccg cccgcagcgg ttgctcggcg ccgtcgatgg cctgaccctg
     1501 gtcgacctcc ccgacgccga ccagtgctgc ggcttcggcg gcacgtttgc cgccaagaac
     1561 gcggacacgt cgctcgcgat ggggaccgac aagatcgccg cggtcgccgc caccggcgcg
     1621 tcggtcctga cggccgccga cacgtcgtgc ctgatgcacc tcgggggcat gctgtcgcga
     1681 gcgggatcgc cggtccgcgt gatgcatctg gccgagatcc tcgcggccga gccggtcgca
     1741 tgaccgcccc gccctccgag ccggcacgcc tgccggcgac cccgaccttc gccggcacgc
     1801 cgcccttccc cgccgcggct cgggcggccc tcgccgacgg ccagctgcgg accaacctcg
     1861 ccgcggcgac gaagaccatc cggaccaagc gcgcgaaggc ggtcggcgaa cgcgcggact
     1921 gggaggcgct gcggctcgcc ggggcggcga tcaaggacga ggtgctcggc gacctgccgg
     1981 cgctgctcga gcaactcgag gcgaaggtca ccgaggctgg cggcaccgtc cactgggcgc
     2041 gcgacgcggc ggaggcgaac gcgatcgtcg cccgccttgt gcatgccgcc ggcgccgacg
     2101 aggtgatcaa ggtcaagtcg atggccaccc aggagatcga gctcaacgag gcgctcgccc
     2161 gcgacgggat cgccgcttgg gagaccgacc tcgccgagct catcgtccag ctgggcggcg
     2221 acctgccctc ccacatcctg gtgcccgcga tccaccgcaa ccggcgagag attcgcgaga
     2281 tcttcgagcg cgagatggcc cgtgcgggcc ggccggcgcc cgcggacctg accgacgacc
     2341 cgcgcgccct cgccgaggcc gcccggctgc acctgcgcga gaagttcctg cgggcgaagg
     2401 tggcgatctc gggcgccaac ttcgcggtcg ccgagaccgg caccgtgatg gtcgtcgagt
     2461 ccgagggcaa cggccgcatg tgcctgaccc tgcccgagac gctgatcacg gtgatgggca
     2521 tcgagaagct ggtgccgcgc tggcaggacc tcgaggtctt cctccagctg ctgccccgct
     2581 cctcgacggc ggagcgcatg aacccgtaca cgtcgatgtg gacgggcgtg actccgggcg
     2641 acggcccgca ggcgttccac ctgatcctgc tcgacaacgg ccgcacggac acgctcgtcg
     2701 acccggtcgg ccggcaggcg ctgcggtgca tcaagtgctc ggcctgtctc aacgtctgcc
     2761 cggtctacga gcggaccggc ggcaaggcct atgggtcgcc atacccgggg ccgatcggcg
     2821 ccattctcac gccccagttg cgggggttga acgtccatcc ggtcgatgcc cagacggcgt
     2881 cgctccccta cgcgtcgtcg ctgtgcggcg cgtgtttcga ggtctgcccg gtccggatcg
     2941 agatcccgga gatcctcgtc cacctgcggg cgacggtcgt cgaacagcac ggctggcgcg
     3001 gccgggtgtc ggaggaggcg gtcctgatgg ccgcggccgg atggacgttc cgggggtcgt
     3061 cacggctcgg ggccgccgag cgtgcgggcg gcctcggcgg ccggctgctc ggacgaggcg
     3121 gctggatccg gcgggcgccg gggcccggcc tgttgcgggc gtggctccgg ggccgggacc
     3181 ttgcggctcc agcgccggag tccttccggc gctggtggag gcgggtcggt cggacggagg
     3241 tcgagccgtg accgaggccc gggacgcggt cctcgatcgc atccgcgcca ccctcgccgc
     3301 cgcccccgcg gcgccctccc ccgtcccccg cgactacgag acggcgctcg ccggcgaaac
     3361 cgatctggtc gagctgttcg cggagcgggt cgccgactac cgggcgacga tccaccgcag
     3421 ccatgccgac ggccttccgg cgacgatcgc ccgcatcctg gccggctggg acgcgcggac
     3481 gctggtcgtc ccggacgcca tcccggccgc gtggctctcg ggtgcgacgg ccgtggtcac
     3541 gctgcgcgac acgccgccac tgaccaaggc ccagctcgac gcgaccgacg ccgtgatcac
     3601 cgacgccgca gtggggatcg ccgagacggg cacgatcgtg ctcgacgcgc ggccgagcca
     3661 gggccggcgg gcgctgagcc tgctccccga ctaccacctt tgcgtcgtcc acgtcgagcg
     3721 gatcgccggc acggtccccg aggctctcgc gcggctggac gcgggccggc cgctgacctg
     3781 gatcagcggc ccgtccgcga ccagcgacat cgaactgcag cgggtcgagg gggtgcacgg
     3841 cccccgggtc ctggacgtcg tgatcgtcgg ctaggtcgtc gctccggacg cgcggacgac
     3901 gaggaccggg cacgacgcgt ggcgcacgac gtgatcggac accgacccag ccaccattcg
     3961 cccgatccgg ctccggccgt gggagccgac gacgatgatg tcggccgact cggcggacgc
     4021 ggccgcgacg atcgactcgc ccgggtggcc ggtccagacc atgaacgccg cctcgacgcc
     4081 ggcggtccgg ctccgggcga cgaggcgccg cgccgcttcc tggcgatcgt cccggatctg
     4141 gtcccagcgc atggcgcggc ccgacgggac gccggtcccg tcgctcatga gctcgtccgg
     4201 gtcgatgacg ctgacgatca gcagctgggc tccgtgcgct gcggccaggt ccagcgccca
     4261 ggtggcggcg tcaccggacg ccggcgcaag gtccgtggcg agcaggatgc ggcggatccg
     4321 gctcttcgga tccgccgcgc gcacggacga ctgctcggtc aactccctgg cgggctcccg
     4381 gtgcggcgag agcccgccat cgaccgccat gtcagcccgg cgctgtgcta gtgatcgccg
     4441 gtccgccaaa cacccgtccc ttccgccacg gggcgcccag cgccggcagc agataccggt
     4501 cgagcccata ccacccggcg acgcgccagg cgaggatgat cccgatcgcc atcgtgaaca
     4561 gcacggggtt cgtggacgcc gagccggcga gcaggaacga catgttcatg agggccccga
     4621 agaaggccgc gaacccggtc agcgccccga acagcagccc cagcccgacc gcgatctcgc
     4681 cgaccgtgat cagccacgca aaccaggtct cgtggtgccc ggcgatcagg aagttgacga
     4741 agtcgcgata ccactcgtag ctgatcggcg gccggccctc ctcggggacc ttggccgcgt
     4801 tggcccagaa tccggccagc gccgagcccc cgtcgaccca gccggtaccc gtgagcttgt
     4861 gccagcccgc ctcgacccag gcgaagccca ggaacagcct gatcggcagc cagaacaggc
     4921 cggccctggt gttcgagaac aggaagcgcg cgaaccgcgg cccctcgatc tcgacctcgg
     4981 tggcctcggg atcgttgcga tcctcgaaca gtgctaccag cagcacgacg aacgtcaccc
     5041 agaaggcgac cgtcagcacc tgccggagcg cggtgccctc ctcgatctcg agccacgggt
     5101 tgaacagcac gatcaacagc gcggccgcca ggatcccgat cgggaccacc atgcgtcgca
     5161 atgcacccat cgtcccttgc ctccactcac tcgtcgatcg acgttcatcg atctctcgaa
     5221 cggtcggggc catcgtggcc gcgggcattg gagcgcgaag cggcaggagg tcacatggat
     5281 ggggggccga gtgtcacgtc cggccgggac cgtcgcgggc accgatcggc ggaagcgcgg
     5341 acaatgggtg cgccgtggcg acgatgatcc ggggacgcgc gcacctcgcc gccctgaccg
     5401 tcctggcggt gatcgccggc gcgtgcggaa gcgaggcgcc gacccaggcg gtgtcatcga
     5461 cggccccgac cccggtcgcc gtcgcctccc cgggcccatc cgacccgacc gatgcgggcg
     5521 ggacgaccgc ggcggacggc tcgtgcgtcg ctcggaccct cgacgccatg accgaggcgc
     5581 agcggatcgg ccagctgttc atgatccagc tgccgaagga ccagctggac gcgaagcacc
     5641 gcgcggcgat cgaggatgac cacttcggct ccgtctggtt cggccgcagc acggcgggcg
     5701 tcgaggcgct gcgcgcggtc tcgaacagcg tccagcgttt ggccacgccg gccgcgacga
     5761 acggcgttcg gttcctgatc gcggccaacc aggagggcgg gctgatccag ggcctgtccg
     5821 gaccagggtt cgccacgatc ccgtccgccg tcgaccaggg ccggatgacg ccggctgcac
     5881 tcaccgcggc cgcggtcacc tgggcgcacg agctccggcg ggcgggcgtc aacgtcaact
     5941 tcgccccgac ggccgacgtc gtcccacccg ggggtgacgc gacgaacgcg ccgatcggcc
     6001 agctcgatcg ggagtacggg caccacccgc gggtcgtcgc cagccacgtc gcggccttca
     6061 tcggcggcat gcgcgccggg ggcatcgcca cgacggcgaa gcacttcccg ggcctcggcc
     6121 gggtcgaggg caacaccgac cacacggccg acgtggtcga caccgtcacg acgcccgacg
     6181 acgcgtacct gcgccccttc cggcgggcga tcgacgatgc cgtgccgttc gtgatggtct
     6241 cgctcgcaac gtacgagcgc atcgatgcct cggcgatcgc ggccttctcg ccgaccatcg
     6301 tcacggggct cctgcgcacg acgctcggct tcaggggcgt gatcatgtcg gacagcctca
     6361 cggccgaggc cgtggcggcg atcccgccgg ccgatcgcgc catccgcttc atcgcgtcgg
     6421 gcggcgacct gatcgtgctc ggccgcctcg cgacggcgat ctcgatggcg aaaggcctgg
     6481 cgagcctcgc ggcgacgagc ccggccttcg cgacgaccat cgatgcggcg gcgaggcgcg
     6541 tgctgcgggc gaaggcggcg gccggcctgg tcgactgcgg ctcgtagggc taccgaatcg
     6601 gaagaccggc tgcccgaagg cagccggtcc tgttcgcggc ggtggtggcg acggccgatc
     6661 agacgggtcc gcggcccacc ctgcgggtgc cggtgaccag gctcacgacg acgaccacga
     6721 tgaccgcgcc gatgaccgag gtcacgatgg tgctcaggtc gaacgcgccc tggagcgggt
     6781 ccttgacgtt gaacagcagg ctggccagga agccgccgac gatcgcgccg acgatcccga
     6841 ggacgatgtg gccgatgacg cccagccctt cgtcccctcg gacgaggaac ccggcgaggt
     6901 acccggcaat ggcgccgagg acgatccagg caatcaggtt catgagtttg acctccatcc
     6961 cagttctcgg ttgatgcccg tgacgggcga cgtggaggcg atcgtcgggg tgggatggtt
     7021 gcgcccatgg caagggacgg gcactgcgtc ttgcgttccc attgcgcgcc gctgcttgtc
     7081 atgccactgc aagaggcggg agccgccgcg gaccgcccat tccacgcctg ctgaagcacg
     7141 gtggtggggc gtaccacggt gacccgccat gaagaaccag ccgctcgtgc tcgtcgccga
     7201 cgacgagccg cccatcaccc gcctcgtggc cctcgcgctg tcggccgact tccgtgtcgt
     7261 gacggccacg ggcggcgagg aggcgctcca gatcgcggac caggccaggc ccgacatcgt
     7321 cctgctcgac gtcctcatgc cggacgtcga cggcatcgag gtcatgcgcc ggctgcgcga
     7381 gtggcgcccg gtcccggtga tcctgatgac ggccagtggg tccaccgccc agcggacgag
     7441 aggcctcgat ctcggtgccg acgactacgt cgcgaagccg ttccaccccg acgagctggc
     7501 cgcccgcgtc cgggcggtgc tccggcgtgc ttccggcgtg gcggccggca gcgtgcccgt
     7561 gcgcgtgggc gacatcgaga tcgacctcga gcggcggcac gtgacccagg gcggtcgcgc
     7621 cgtgtccctc agccgcccgg agtggctgct gctccaggcc ctggccacga acgccggccg
     7681 ggtcgtgttg cacggcgagc tgctgaccga agtctggggc ccggagttcc gcgacgacgt
     7741 gcagtacctg cgcgcgtgga tcagccgcat ccgccgcagg ctgggcgcgc aacccggcga
     7801 ggcgggaccg atcgtgacct ttcagggcat cgggtacctg cttgatgtgg agcaaccgcc
     7861 cgcagcgcgg acgtaagccc gctgcaagcc ggacggggtc cgcgtcgcga ctgcgtgcaa
     7921 ccacgtccgc cgcatgctcc cagcgggagg caccgcctcc cgctgtactc gactggagac
     7981 acccatgacc accgccagca ccacgagcgc ggcgcgccgc gctccggatg aagccaggga
     8041 gagggagtcc gtcgcggccg cgcgatcgag ccttgcggac accgtcgatg cggtccgttc
     8101 gactgcgagc agcgtcgggg accgcctgcc cgaggtgatc gagacgatga agtcgagcgc
     8161 cgccgacggc gcgcgatcca tgcaggcctg gtccgcatcc cggcagcgcc tcgcagttgc
     8221 cttctcactg ggcctgggta tgggcctcac gctggccggt gcgccccgcc tcgcgatcgc
     8281 gagcgcgctg gtgccggtca tcgtggtcgc ggccaccatc gtcgaacgta acgagggagc
     8341 gtcctgatgc taacccgaac gatccgtgtg aacggaacca ccatcgaccc ggccgaggcg
     8401 attcgctcga ccggactcga cacgatgccc gaacgcgtcg atgccgccat caacgcggcc
     8461 ggcgaatcgc tgcgcgatgc gggcgactcg ctgcgcgcga ccgtgcacca gctctcggcg
     8521 ccaccgtccc gcacgaggat gccatcgacg cgcggctggc tggccggatt cgcgttcctc
     8581 gtcgccgcga tcgccctcgc gacgtggctg ttccgtcgca tgtcggctgc gccgatggcc
     8641 ctcagcgcgg atgacctcga cgccctcgac ctcgaggatc tcgagcgagc caagggcgag
     8701 gggatgggca ccgcggtggg caccgccatc gcgagcgatc cgctgcacgt cccgtcggac
     8761 aacgacctga tgggcgtgat ggccgagccc gccgcgattg gcgagcacaa cggtcgctag
     8821 gccgatgcgc agcacccgcg ccggcccggc caaggctgtg gagacctggc agatgccggt
     8881 ccagctggcc gcggctggcg cgatcatcgt cgtcgccgcc gaggacgagc gcttcggcgg
     8941 cgcgttgcgc cgggcgaccg agctcgcgac caagcgcggc gaaccgctcc tcctctacga
     9001 ctgggacgcc ccctccttcc ttgccgagcc gctgccgacc tggtggtcga gcgagggctc
     9061 gcgcgaccag cttcccgatc gcctcgatcc ggagcagctg cggacggccg gtcggggagc
     9121 gctggccgcc caggtcgatc gcgcgcgcga cagcggcgtc gaggcgttcg gctggctccc
     9181 gtctgaccac ggcccaggac cgctggcgga ctacgccggc gcgcagggag catcgatcgt
     9241 cgtcgtaccc cgcgacctca ccgagctcca cgggctcgat gccctgatca acggcacggc
     9301 gcgccccgcg gacgcgctgg tcgagcgcgc ctcggccgcc gtcgtcatcg tctgagtcct
     9361 cgcaccgagg gggcgcc
//
LOCUS       DBNHXO010000468         8206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1539349, whole genome shotgun sequence.
ACCESSION   DBNHXO010000468 DBNHXO010000000
VERSION     DBNHXO010000468.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 8206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8206
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1539349"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="AC162A_09440"
     CDS             <1..624
                     /locus_tag="AC162A_09440"
                     /inference="COORDINATES: protein motif:HMM:NF015013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HIR5011107.1"
                     /translation="PIEPLTFLFFTRVASTLVFGSVTAVSFARNFQSVPVALIGVAIS
                     LAVFPTLSASWAAGDRAGFSRQVRNSAATIGVVSILAAIGLIVVGPLAIDVLLGGGAF
                     DAEDVRLTATVLAAFALAVPFDALGHLLARSLYATHNTLLPLGAAVAGFAVTIVVTLA
                     LVNPVGIVAIPLGFAAGLAVRTAVQAVALAYRLRSQPPAAAADPLPA"
     gene            complement(621..1532)
                     /locus_tag="AC162A_09445"
     CDS             complement(621..1532)
                     /locus_tag="AC162A_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011108.1"
                     /translation="MTRRRRFRRRHGFAILLALGLLVVGAIWTNVLGVGDKLQNVAAR
                     IELLIDPPPDRPIAAIVKVTPRPEVAETEAPPGPEESLAPGATPRPTPTPKPERVKVD
                     VNLFKHPENHFISEEFNHEWCAVAGTQMVLEIHGKAPLTLNFQKDLASRIGEWESRRD
                     SRNGGWGPSAMVAALEAYGVNGYEVRAYDTRADALYDAAKAISKLHAPVILLSWRGAH
                     TWIMTGYRADADPLVFDNAKVSGTYILDPWYPLVSSIWGASDPPGTYQDGDEMRRNYL
                     PWNRPEGNYPDRDGLFIAVVPTKTLNR"
     gene            complement(1609..1923)
                     /locus_tag="AC162A_09450"
     CDS             complement(1609..1923)
                     /locus_tag="AC162A_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011109.1"
                     /translation="MRIRAAKIEELTPKPRARALSPRQLAALQREEEIRKALTRLKSD
                     EDVIALELDPAEKIPTMRAAVKRAIAAHKPGTNMAIRGRTIYLSTGRLPGGRGGRRPK
                     AR"
     gene            2230..2910
                     /locus_tag="AC162A_09455"
     CDS             2230..2910
                     /locus_tag="AC162A_09455"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR5011110.1"
                     /translation="MTDPAGGAPTPPPPEPTPAPPAGAPPASSWNTPPPQATAPAGFQ
                     MAQAEAGPAPGVSYADTVMRVVAYIIDSVILGVAIALVAIILGSIAFGIAVGGGFLIS
                     IIIVVVIAVIALLGSAFYFVYFWQTLRASPGQKILNLETVNAADGATLTREQAIRRWA
                     FLFGPQVVASVFQFSGSVSGALGSIGSLLGLLAFLYAIYLLYTVTQNPKRQGFHDVQA
                     NTVVVKRG"
     gene            complement(2965..3378)
                     /locus_tag="AC162A_09460"
     CDS             complement(2965..3378)
                     /locus_tag="AC162A_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR5011111.1"
                     /translation="MAILLDPSAITAAADTADLNHRAAIAWFERVDEPLLLGALGLAE
                     LDLVLQRELGPDATQAVLASVVAGSIRVVAPTPDDIARAAQLLGEAAEHRPRLADALL
                     IATAERLSIRRVAAFDRRPLAVFRPRHVRSLEFEP"
     gene            complement(3378..3617)
                     /locus_tag="AC162A_09465"
     CDS             complement(3378..3617)
                     /locus_tag="AC162A_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HIR5011112.1"
                     /translation="MRTTILLDADLLERLDRHVGRQHTTKTSVIAAALETWLDSHEAT
                     PEFPFLGVGRSSHGRLSLDGRSIARREAARRPGER"
     gene            3711..4883
                     /locus_tag="AC162A_09470"
     CDS             3711..4883
                     /locus_tag="AC162A_09470"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR5011113.1"
                     /translation="MSRFEPNLRIPGPTALPPSVREAGGRQMVNHRGEEFKALLLRVG
                     EGMKPFFGTTSDVILLTCAGTGGLEAAIVNTLSPGDAVLAVTMGAFGDRFAKIAEVYG
                     ASVTRLEVEWGEAARADDVRRAAGQIPGLRAVLLTHNETSTGVTNDVASLAASIREVA
                     PEALILVDAISALGAVPFAMDAWGLDLVVTGSQKAWMAAPGMAMAAVGPRAWAAGETA
                     RMPRFYLDLKRHRDTAAAGETPWTPAVAVMYQVDEGLRLMQAEGDGVFARHVACAAMT
                     RAGLRAIGFQLLAADDVASKTVTAAWIPEGLDWSAFNKALKARGLVLAGGQGKLKGRI
                     FRLGHLGSVTTDEILAAIGVIELVAIEHGLDVEAGAAVAAAQRVVAGARLAVKVPA"
     gene            4880..6463
                     /gene="serA"
                     /locus_tag="AC162A_09475"
     CDS             4880..6463
                     /gene="serA"
                     /locus_tag="AC162A_09475"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR5011114.1"
                     /translation="MRILVAESLAAEGIELLRASHDVDEKLGLDRAELAAILPDYDAL
                     VVRSQVKVDAELIAAGTRLVVIGRAGVGVDNVDLDAATRAGITVVNAPTGNTIAAAEH
                     TLALLYGLARKTAEADASMRRGEWKRAAFTGVELRGKTLGIVGLGKIGQAIAVRARAM
                     EMTVIGSDPFVTPDQAANVGVELVAFDELIARADAITVHVPMTRATRGLINAATIERM
                     KPSVLLLNVARGGILDEADVAEALRSGRIAGAAIDVFEAEPPTGSPLLDAPNTLLTPH
                     LGASTAEAQVLVAEEVAEQILEVLEGRPARYAVNAPLLTPETAQAIAPYLPLAETLGR
                     FLGQFAHAAPRTLTLEIAGEPAAYDASPLVAAVLRGLLETSTTERVNLVNAATMARSR
                     GIKVVERKTSTAGAFASLLTVTGESDGRTATVAGTVANGEPHIVRLDEHAMDLAPADS
                     MLITRHQDRPGTMGRIGVLLGEADVNISAMTLARSAPRADAFMVLALDDDVPAAVAQA
                     IRTDPAVIDVWTIRLGGER"
     gene            6460..7299
                     /locus_tag="AC162A_09480"
     CDS             6460..7299
                     /locus_tag="AC162A_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR5011115.1"
                     /translation="MTSADTANSLIPEGLDATLVLLRHGESELIREGRFQGQAETPLS
                     AVGRRQAALAAERLATPHASPMLPIPGGGPLEIVHSPLARTTETAALVAAAIGSGPPG
                     GPSPSVRPEPGILELAQGVWVGETHDEIVRRWGAELAAWRRRPEEAWAPGGESLAEVQ
                     ARSRPALAGILERLGRDYPRGSLDRPQVGGYRGAGLDTSQPWSILVGHDGVFKVVLLT
                     LFDIPLSRFWTFSFALCGISVVEFRGGRAVLRAHNLTEHLAPLLDEEAIAEAEARARS
                     GAL"
     gene            complement(7296..>8206)
                     /locus_tag="AC162A_09485"
     CDS             complement(7296..>8206)
                     /locus_tag="AC162A_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4129 domain-containing transglutaminase
                     family protein"
                     /protein_id="HIR5011116.1"
                     /translation="QVKRQARSQDPYDLATEIVKILGNQSVYKYETDITDLNCTVSQV
                     ECFARYKRGYCLHYASTMAILLRAANPDNPIPTRLVEGFLPGDRVGNIETVKNKGAHA
                     WVEVYFPGFGWVGPFDPTGPGVAAPPSVIKDGPAVSAGPVATIPPNERGGDPTRPPLV
                     GGDTIPTPPGGQPPNRALFIVLTVLLVLLVGGIALAAWLRGPRGEISPDRAWQSMTKA
                     ASQLGYGRRANQTVYEYAATLGELVPVAKADLATVADAKVETAYARVRLGGDRLHAVG
                     QATRRLRVSLLRLVFRRDRRLRRRRR"
BASE COUNT         1110 a         2948 c         2939 g         1209 t
ORIGIN      
        1 ccgatcgagc cgctgacgtt cctgttcttc acgcgcgtgg ccagcacact cgtcttcggc
       61 agcgtgacgg cggtcagctt cgcccggaac ttccagagcg tgcccgtcgc cctgatcggc
      121 gtggcgatct cgctggccgt gttcccgacg ctctcggcct cgtgggcggc cggcgaccgg
      181 gccgggttca gccgccaggt ccggaacagc gcggcgacga tcggggtcgt gtcgatcctc
      241 gccgcgatcg gcctgatcgt cgtcggcccg cttgccatcg acgtgctgct gggcggcggc
      301 gcgttcgacg ccgaggacgt gcggttgacg gcgacggtgc tcgcggcctt cgccctcgcc
      361 gtcccgttcg atgccctcgg ccacctgctg gcgcggagcc tgtacgccac ccacaacacg
      421 ctgctgccgc tgggcgccgc cgtggccggg ttcgcggtga cgatcgtggt gacgctggcg
      481 ctggtgaacc ccgtcgggat cgtcgccatc ccgctgggct tcgcggccgg cctcgccgtg
      541 cggaccgcgg tccaggcggt cgccctcgcc taccgcctcc gctcgcagcc gcccgccgcg
      601 gcggcggatc ccctgccggc ctagcggttc agcgtcttgg tcgggacgac cgcaatgaac
      661 aggccgtcgc gatcgggata gttgccctca ggcctgttcc acgggagata gttgcgtcgc
      721 atctcgtcgc cgtcctggta ggtgccgggc gggtcagacg ctccccagat cgaggacacg
      781 agcggatacc acgggtcgag gatgtaggtg ccgctgacct tcgcgttgtc gaacacgagc
      841 gggtcggcat cggcccggta cccggtcatg atccaggtgt gggcgccccg ccacgacagg
      901 aggatgaccg gggcatggag cttcgagatg gccttggcgg cgtcgtacag cgcgtctgcc
      961 ctggtgtcgt acgcgcggac ctcgtagccg ttgaccccgt aggcctcgag ggcggcgacc
     1021 atggccgacg gtccccagcc accgttgcga ctgtcgcgcc tgctctccca ctcgccgatt
     1081 cgcgacgcca ggtccttctg gaagttgagg gtcaggggcg ccttgccgtg gatctcgagg
     1141 accatctgcg tgccggcgac ggcgcaccac tcatggttga actcttcgct gatgaaatga
     1201 ttctcggggt gcttgaacag gttgacgtcg accttgacgc gttccggctt cggcgtcggc
     1261 gtgggccgcg gcgtcgcacc tggcgcgagg gactcttcgg gtcccggtgg cgcctcggtc
     1321 tcggctacct cgggccgcgg ggtgaccttg acgatggccg cgatcggccg atcggggggc
     1381 ggatcgatca gcagctcgat tcgcgcggcc acgttctgca gcttgtcgcc cacgcccagc
     1441 acattcgtcc agatggcacc caccaccagc aggccgagcg cgagcaggat cgcgaagcca
     1501 tggcgtcgtc ggaagcgtcg gcgtcgggtc acaggatcac tccggtcggg gcgggcggcc
     1561 gaagagacgc gtgggacgcg ggcactgcga cgcgggcggg ccgcccgatc agcgcgcctt
     1621 gggccgccgg ccgccgcggc cgccgggcag gcgcccggtc gagagataga tcgtccggcc
     1681 ccgaatcgcc atgttcgtgc ccggcttgtg ggcggcgatc gcgcgcttca cggccgcgcg
     1741 catggtcgga atcttctcgg caggatcgag ctcaagcgca atgacgtcct cgtcggactt
     1801 caatctcgtc agcgccttgc gaatttcctc ctcgcgctgg agcgccgcaa gctgacgcgg
     1861 cgacagcgcg cgcgcacgcg gtttcggggt gagctcttcg atcttcgcgg cacgaatgcg
     1921 catggcctgc ggaaactcct gcgtcaaatc tggcggtggg ccacgggatg atgacacgaa
     1981 tccgtccggg ggccaatcag attcatcatc ggtgcgttcc gtcgagcaca ttcgcctagc
     2041 aagcgcatcg cggcacgcag cagattcacg gttccgcttc atcgtgtgga tggttcctga
     2101 acgcaccgtc accgtgggtg tgcccgggcc acagcgagga caacgcgcgg cggggcttcc
     2161 ttaacgtgct cgcgcgccgt atcctgagcc ggacgcggcg cgctcgcgcc ggcgagggag
     2221 aggtgttcga tgacggatcc cgcgggaggg gcgcccacgc ccccgccccc tgagcccacc
     2281 cccgcaccac cggcgggagc cccaccggcc tccagctgga acacgccgcc tccgcaggcg
     2341 acggcgccgg ccggcttcca gatggcccag gccgaggccg gacccgcgcc gggcgtgtcg
     2401 tacgccgaca ccgtgatgag ggtcgtcgcg tacatcatcg actcggtcat cctcggggtc
     2461 gccatcgcgc tcgtcgcgat cattctcgga agcatcgcgt tcggcatcgc cgtgggcggc
     2521 ggcttcctga tctcgatcat catcgtggtg gtcatcgctg tcatcgccct gctgggcagc
     2581 gccttctact tcgtctactt ctggcagacc ctgcgcgcgt cgccgggaca gaagatcctc
     2641 aacctcgaga ccgtgaacgc cgctgacggg gcgaccctca cgcgcgagca ggcgatccgc
     2701 cgatgggcgt tcctgttcgg cccgcaggtg gttgccagcg tgttccagtt ctcgggttcc
     2761 gtgtcgggcg cgctcggctc gatcgggtcg ctgctcggcc tgctggcctt cctgtacgcg
     2821 atctacctgc tgtacacggt cacccagaac cccaagcgcc agggcttcca cgacgtccag
     2881 gccaacacgg tggtcgtcaa gcgcggctga tccgcgacac gcgcaacgca cagacggggc
     2941 cggcaccgcc ggccccgttt cgattcaggg ctcgaactcc aggctccgga cgtggcgcgg
     3001 gcggaacacg gccagcggcc gccggtcgaa ggcggccacg cgccggatcg agagccgctc
     3061 ggcggtggcg atcagcagcg cgtcggcaag gcgcggacgg tgctcggcgg cttccccgag
     3121 cagctgggcg gcccgcgcga tgtcgtcggg cgtcggggcg acgacacgga tcgagccggc
     3181 tacgaccgaa gcgagcaccg cttgcgtcgc atcgggcccg agctcgcgct ggagcacgag
     3241 atccagctcc gccagcccga gcgcacccag gagcagcggt tcgtcgacgc gctcgaacca
     3301 ggcgatggcg gcccgatggt tcaggtccgc cgtgtcggcg gccgccgtga tcgccgacgg
     3361 atccagcagg atcgccacta gcgctcgccc ggcctgcgcg ccgcctcccg gcgggcgatc
     3421 gagcgcccgt cgagcgagag ccgtccatgc gagctgcggc ccaccccgag gaacgggaac
     3481 tccggcgtcg cctcgtgcga gtccagccac gtctcgagcg cggcggcgat caccgaggtc
     3541 ttggtcgtgt gctggcgacc gacgtggcgg tcgaggcgct cgagcaggtc ggcgtcgagg
     3601 aggatcgtgg tccgcaccgt cttcatatga cgtcatatct accacgctgg acggacccct
     3661 gtcacctccc ccaccatcag cccagcatcg ccgtgatcag gagccgtgac gtgagccgat
     3721 tcgagcccaa cctccgcatc ccggggccta ccgccctgcc gccctccgtc cgggaggccg
     3781 gcgggcgcca gatggtcaac caccgcgggg aggagttcaa ggcgctgctc ctccgcgtcg
     3841 gcgagggcat gaagcccttc ttcggcacca ccagtgacgt catcctcctg acgtgcgcgg
     3901 ggacgggcgg gctcgaggcc gcgatcgtca acacgctgtc gccgggcgac gcggtcctcg
     3961 cggtgacgat gggcgcgttc ggcgaccggt tcgccaagat cgcggaggtc tacggggcgt
     4021 ccgtcacgcg tctcgaggtc gagtggggcg aggccgcacg ggccgacgac gtccggcgcg
     4081 cggcggggca gatccccggc ctgcgggcgg tcctgctcac ccacaacgag acctcgaccg
     4141 gcgtgaccaa cgacgtcgcc tcgctcgcgg cctcgatccg cgaggtcgca cccgaggcgc
     4201 tgatcctcgt cgatgccatc tcggcgctgg gcgccgtgcc gttcgcgatg gatgcctggg
     4261 gcctcgatct cgtggtcacg ggatcacaga aggcgtggat ggcagcaccc ggtatggcga
     4321 tggcggcggt tggaccgcgc gcctgggccg ccggggagac cgcacgaatg ccgcgcttct
     4381 acctcgacct caagcggcac cgcgacaccg cggccgccgg cgagacaccc tggacaccgg
     4441 ccgtggcggt catgtaccag gtcgacgagg ggctgcgact catgcaggca gagggcgacg
     4501 gcgtgttcgc gcgccacgtg gcctgcgcgg cgatgacccg ggccggcctg cgggcgatcg
     4561 ggttccagtt gctcgcggcg gacgatgtcg cgtcgaagac ggtcaccgcg gcgtggattc
     4621 ccgagggtct ggactggtcc gcgttcaaca aggcgctcaa ggcgcgcggg ctggtgctgg
     4681 ccggaggcca gggcaagctc aagggccgca tcttccggct gggtcacctg ggatccgtga
     4741 cgacagacga gatcctggcc gcgatcgggg tgatcgaact ggtcgcgatc gagcacggcc
     4801 ttgacgtcga ggccggggcc gccgtggccg ccgcgcagcg ggtcgtcgcc ggcgcgcgcc
     4861 tggccgtcaa ggtgcccgcg tgaggatcct cgtcgccgag tcgctcgcgg cggagggcat
     4921 cgagctcctg cgcgcgagcc acgacgtcga cgagaagctc gggctcgacc gggccgagct
     4981 ggcggcgatc ctgcccgact acgacgccct cgtcgtccgc agccaggtca aggtcgacgc
     5041 cgagctgatc gcggccggga cgcggctcgt cgtcatcggc cgggcgggcg tcggcgtcga
     5101 caacgtcgac ctcgatgcgg cgacccgcgc cgggatcacg gtcgtgaacg ccccgacggg
     5161 caacacgatc gcggcggccg agcacaccct ggcgctgctc tacgggctcg cccgcaagac
     5221 cgccgaggcc gacgcctcga tgcgccgcgg cgagtggaag cgcgccgcgt tcaccggggt
     5281 cgagctgcgc ggcaagaccc tcggcatcgt cgggctgggc aagatcggcc aggcgatcgc
     5341 cgtccgggcc cgggcgatgg agatgacggt catcgggtcg gacccgttcg tcacgcccga
     5401 ccaggccgcc aacgtgggcg tcgagctggt ggcgttcgac gagctcatcg cccgcgccga
     5461 tgccatcacc gtccacgtgc cgatgacgcg ggcgacgcga ggcctcatca acgccgcgac
     5521 gatcgagcgc atgaagccgt cggtgctcct gctcaacgtg gcgcgcggcg gcatcctcga
     5581 cgaggcggac gtcgccgagg cgctgcgatc gggacggatc gcgggggccg ccatcgacgt
     5641 gttcgaggcc gagccaccga ccggctcgcc gctgctcgac gcgcccaaca ccctcctgac
     5701 gccccatctc ggcgcctcga ccgctgaagc gcaggtcctc gtggcggagg aggtcgccga
     5761 gcagatcctc gaggtcctcg agggccggcc cgcacgctat gccgtcaatg cgccgctgct
     5821 gacgcccgag acggcgcagg cgatcgcccc ctacctcccg ctcgccgaga ccctcggccg
     5881 gttcctcggc cagttcgccc acgcggcgcc gcgcacgctg accctcgaga tcgccggtga
     5941 gccagcggcg tacgacgcgt cgccgctcgt cgcggcggtc ctccggggcc tcctcgagac
     6001 gtcgaccacc gagcgggtga acctcgtcaa cgcggcgacc atggcccgct cgcgcggcat
     6061 caaggtggtc gagcgcaaga cctcgaccgc gggcgcgttc gcgtcgctac tcacggtcac
     6121 cggcgagagc gacggccgga ctgcgaccgt cgcgggcacc gttgccaacg gcgagccgca
     6181 catcgtccgt ctcgacgagc acgcgatgga cctcgcaccc gccgactcga tgctcatcac
     6241 ccgccaccag gaccgccccg gaacgatggg ccggatcggg gtcctgctgg gtgaggcgga
     6301 cgtcaacatc agcgccatga cgctggcccg ctctgcgccg cgcgcggatg cgttcatggt
     6361 gctcgcgctc gacgacgacg ttccggcggc ggtcgcgcag gccatccgca ccgaccccgc
     6421 cgtgatcgac gtctggacga tccgcctcgg cggcgagcgg tgacctcggc cgacacggcc
     6481 aactcgctca tccccgaggg cctggacgcg acgctcgtcc tgctgcgcca cggcgagtcg
     6541 gagctcatcc gggagggtcg cttccagggc caggcggaga cgccgctgtc cgccgtaggg
     6601 cgccggcagg cggcgctggc ggcggagcgg ctggcgacgc cccacgcgtc gccgatgctg
     6661 ccgatccccg gcggcggccc gctcgagatc gtccactcgc cgctcgcgcg aacgaccgag
     6721 acggcggccc tggtcgccgc cgcgatcggg tcaggaccgc ccggtggccc gtcgccatcg
     6781 gtccggcccg agccagggat cctcgagctc gcgcagggcg tgtgggtggg cgagacccac
     6841 gacgagatcg ttcgccgctg gggagccgag ctcgccgcgt ggcggcgccg gccggaagag
     6901 gcgtgggcgc cgggcggcga gtcgctcgcc gaggtccagg cacgctcccg cccggcgctc
     6961 gccggcatcc tcgagcgcct gggacgcgac tacccacgcg gcagcctcga ccgcccccag
     7021 gtcggcggct accgcggcgc cgggctcgac accagccagc cgtggtcgat cctcgtcggg
     7081 cacgacggcg tgttcaaggt cgtgctccta acgctgttcg acatcccgct gagccgattc
     7141 tggacgttct cgttcgccct gtgcgggatc tccgtcgtgg agttccgcgg cgggcgggcc
     7201 gtgctgcgtg cccacaacct caccgagcac ctggcgccgc tgctcgacga ggaggccatc
     7261 gccgaggccg aggcgcgcgc gaggtcgggc gcgctctagc ggcgtcgccg gcgcagccgg
     7321 cggtcacggc ggaagaccag gcgcaggagc gagactcgca ggcggcgcgt cgcctggccc
     7381 acggcgtgga gccggtcgcc gccgagacgg acgcgggcat acgcggtctc gaccttcgcg
     7441 tcggcgaccg tggcgaggtc ggcctttgcg accgggacca gctcccccag cgtcgccgcg
     7501 tactcgtaca ccgtctggtt ggcgcgccgc ccgtagccca gctgcgacgc cgccttggtc
     7561 atcgactgcc aggcgcgatc cggcgagatc tcaccgcgcg gtccgcgcag ccacgccgcc
     7621 agcgcgatcc cgcccacgag caggaccagc agcaccgtca ggacgatgaa tagcgcccgg
     7681 ttcgggggtt ggccgcctgg cggcgtgggg atcgtatcgc cgccgaccag gggtggccgc
     7741 gtggggtccc ccccgcgctc gttgggggga atcgtcgcga ccggcccggc gctgaccgcc
     7801 ggcccatcct tgatcacgct tggcggcgcc gcgacgccgg gtcccgtcgg gtcgaatggg
     7861 ccgacccacc cgaagcccgg aaagtagacc tcgacccacg cgtgggcgcc cttgttcttg
     7921 acggtctcga tgttgcccac ccggtcgccg ggcaggaagc cctcgacgag gcgggtcggg
     7981 atcgggttgt cggggttcgc cgcccgcagc aggatcgcca tcgtcgacgc gtagtggagg
     8041 cagtagccgc gcttgtagcg cgcgaagcac tcgacctggc tcacggtgca gttcaggtcg
     8101 gtgatgtcgg tttcgtactt gtacacggac tggttgccga ggatcttgac gatctccgtc
     8161 gcgaggtcgt acggatcctg cgaccgggcc tggcgcttga cttgct
//
LOCUS       DBNHXO010000469         4767 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2132459, whole genome shotgun sequence.
ACCESSION   DBNHXO010000469 DBNHXO010000000
VERSION     DBNHXO010000469.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2132459"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="AC162A_09490"
     CDS             complement(<1..683)
                     /locus_tag="AC162A_09490"
                     /inference="COORDINATES: protein motif:HMM:NF047309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5011117.1"
                     /translation="MSRAAPASAEPELGSPDPSLRYLLGRLSLVERLVRSAVDRRRAV
                     DADPGDRFRGLYISDEQVDGLLDHASGPLVPQVDEADLTLRAALEARASEEEQAGAGL
                     RLRTMARAFDLEPIDVELLLIALAPDLDPRFERLYGYLHDDVSRRRASVGLAIELCSP
                     AAASDGARIRARLGPMAPLLAGRLLLIEDVDRPSLTRPLRVPDRVTAHLLGDDSPDPV
                     VEALLTPSVA"
     gene            complement(691..1416)
                     /locus_tag="AC162A_09495"
     CDS             complement(691..1416)
                     /locus_tag="AC162A_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pvc16 family protein"
                     /protein_id="HIR5011118.1"
                     /translation="MIYDVDQLLEKLVKRDALNGSSVDLVFDAPTKDWVARRSGPAVD
                     LYLYDIREDIARRVPAWEDVRDPGGTVTRRRQPPRRFRLAYLVTAWTQRPEDEHRLLS
                     SLLACFLRNPMIKPEDLDDPLNEADLPVYLEVAQPPSQERSLADVWSALGGELKPSLD
                     LVVVAPMVVDSSTYFGPPIQEGPSIGIASTNGASERRSGGRKAEGPLEIEPSTTELLP
                     ANAGAKDGGVRLRVSGVRRPPHR"
     gene            1602..3104
                     /locus_tag="AC162A_09500"
     CDS             1602..3104
                     /locus_tag="AC162A_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018552060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HIR5011119.1"
                     /translation="MPNYLSPGVYVEEVEAGSRPIEGVGTAVAAFVGLAARGPANAPT
                     LVTNWNQFTSTFGDFIEGSYLAHAIYGYFLNGGGAAYVVRVGADGVAPTASAELPSST
                     DKAKPAFRVAALEAGPEGNDLSIDVQPAGEEGNFKLVVKRGGKEVETFDNVSTARGKQ
                     NVATIVKAQSKTITIEDVGALAPAAGSTALVGGSAAAPSRLTPDDYVGNSSDRTGFAG
                     LEAVDEVTMLSVPDLMAVYEQGMIDLEGLQAVQLAMIAHCELMGDRVAILDAPPGLNA
                     QQVKEWRVDKAGYDSKYATLYWPWIKVFDPLSGQAKFVPPSGHTAGIWARNDDTRGVH
                     KAPANEVVRGAISLALNLTKAEHDQLNPNGINCIRAFPGRGIRVWGARTISSDPSWRY
                     LNVRRLFNYIEESIFEGTQWVVFEPNDQRLWGRVKRTINAFLLRVWRDGALFGATPDQ
                     AFFVKCDEENNPPEVRDAGQLIIDIGIAPVKPAEFVVFRIAQFSGSPDAE"
     gene            3144..3578
                     /locus_tag="AC162A_09505"
     CDS             3144..3578
                     /locus_tag="AC162A_09505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098015 - virus tail [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIR5011120.1"
                     /translation="MPTTDPLLQGNVLLDIDGVGVVANFTSVSGVSSEVEIVDNPFVN
                     DQGKVTIGKLPGKPKTPTVTLKRGLTGATEIWDWHTAVMQGNPDRRNGSVILMNTIGE
                     EILRYNFTNAMVSKISLSEGGATTSGVQIEEATLACETLVKG"
     gene            3583..4017
                     /locus_tag="AC162A_09510"
     CDS             3583..4017
                     /locus_tag="AC162A_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011121.1"
                     /translation="MTTALLPELGAERLGTNASDALRTEYAFVLPRGFVDGSGTVHRD
                     GVMRLATARDEIIPQRDPRVRENEAYLTILLLSRVVTRLGTLPQVHTGVIEGLFASDL
                     AFLQDLYRRVNQEGHTQASVTCPACNHAFTVDVAGEQRLGES"
     gene            4014..4172
                     /locus_tag="AC162A_09515"
     CDS             4014..4172
                     /locus_tag="AC162A_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6760 family protein"
                     /protein_id="HIR5011122.1"
                     /translation="MTYATDRLFEEVAYVAYHFHWPMDTILDLEHPLRLRFVDEIGRI
                     NRRLSEEE"
     gene            4178..>4767
                     /locus_tag="AC162A_09520"
     CDS             4178..>4767
                     /locus_tag="AC162A_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011123.1"
                     /translation="MRLPWPFGRSASDDGASSTSGDAGAGVTDAVPASPPVPAPIPPT
                     GAWRTLPPIQRTAGPPPVVAPAAPFLAAVPGHAPLPPIVSPLGHESSPVAPAGIVVAH
                     PHAVPSLTSGAPLTTRQVQRHPSADSPAADSPAAAADEGSPVVARAVDAPVVAEVRTV
                     TPPPELDLPPIRTVSPVTPAATVTPSPRPLTQASPTL"
BASE COUNT          707 a         1745 c         1660 g          655 t
ORIGIN      
        1 gccaccgacg gggtgagcag cgcttccacg acggggtccg ggctgtcgtc gcccaggagg
       61 tgggccgtga cccgatccgg gacccgcagc gggcgggtga gcgacgggcg gtcgacgtcc
      121 tcgatgagca gcagccgtcc cgccagcagc ggcgccatgg gccccagccg cgcacggatc
      181 cgggcgccgt ccgaggcggc cgcggggctg catagctcga tcgccaggcc gacgctcgcg
      241 cgccgccgcg agacgtcgtc gtggaggtag ccatacaggc gctcgaagcg cgggtcgagg
      301 tccggcgcga gggcgatcag caggagctcg acgtcgatcg gctcgagatc gaaggcgcgc
      361 gccatcgtgc gcagccggag cccggcgccg gcctgctcct cctcggacgc gcgcgcctcg
      421 agggccgcgc gcaaggtgag gtcggcctcg tccacctgcg ggacgagcgg tccagaggcg
      481 tggtcgagca gcccgtcgac ctgctcgtcc gagatgtaca ggccacggaa ccggtcgccg
      541 ggatccgcgt cgacggcgcg gcggcgatcc accgcggacc gcaccaggcg ctcgaccagg
      601 gacagccgcc cgagcaggta gcgcagggac gggtccggcg agccgagctc gggttccgcg
      661 ctcgccggcg cggcccggct catcgcccgg tcatcggtgc ggcgggcgcc ggacgccgct
      721 gacacggagc ctgaccccgc cgtccttcgc gcccgcgttc gccggcagca gctcggtggt
      781 ggacggctcg atctcgagcg gtccctcggc cttgcggccg ccgctgcggc gctcgctggc
      841 gccgttggtc gaggcgatgc cgatcgacgg tccctcctgg atcggcggcc cgaagtaggt
      901 gctcgagtcg accaccatcg gggccacgac cacgaggtcc agcgatggct tgagctcgcc
      961 gccgagggcg gaccacacgt cggccagcga ccgctcctgg ctgggtggct gggcgacctc
     1021 gaggtagacc ggcaggtcgg cctcgttcag ggggtcgtcg aggtcctcgg gcttgatcat
     1081 cgggttgcgc aggaagcagg cgagcaacga ggacagcagg cggtgctcat cctcgggccg
     1141 ctgcgtccac gccgtgacga gataggcgag gcggaagcgc cgcggcggct gccggcgccg
     1201 ggtcacggtg ccgcccgggt cgcggacgtc ctcccacgcg ggcacgcggc gcgcgatgtc
     1261 ctcgcggatg tcgtacaggt acaggtcgac cgcgggaccg ctcctgcgcg cgacccagtc
     1321 cttggtcggc gcgtcgaaca cgaggtcgac ggacgagccg ttgagcgcgt cgcgcttgac
     1381 cagcttctcg agcagctgat cgacgtcgta gatcactcac cgatcgtcgg ttcagccggg
     1441 ccgcggcgcc agtcggtcgc ggggcatccg tccgggcacc gctggtcgcc ccttcgggca
     1501 atggcgcccg ccctcccgcg cgccctctcg ggccttgttc ggccctcgat tgggtgggac
     1561 ggtcacgtca tcgggatccc tgcatccgga aagggcaacg catgccgaac tatctctcgc
     1621 ccggcgtcta cgtcgaggag gtggaggccg ggtcccgtcc gatcgagggc gttggaacgg
     1681 ccgtcgccgc gttcgtcgga ctggccgctc gcgggccggc caacgcgccg accctggtca
     1741 cgaactggaa ccagttcacg tccaccttcg gcgacttcat cgaaggctcg tacctcgcac
     1801 atgccatcta cggctacttc ctgaacggtg gcggggccgc gtacgtcgtg cgcgtcggcg
     1861 ccgacggcgt tgcgccgacc gcgagcgccg agctgccgag ctcgacggac aaggccaagc
     1921 cggccttccg cgtcgccgcc ctcgaggcgg gtccggaagg gaatgacctg agcatcgacg
     1981 tccagcccgc gggcgaggaa ggcaacttca agctcgtcgt caagcgcggc ggcaaggagg
     2041 tcgagacctt cgacaacgtc agcacggcgc gcggcaagca gaacgtcgcc acgatcgtca
     2101 aggcgcagtc gaagacgatc accatcgagg acgtcggcgc cctggcgccg gccgccggct
     2161 cgaccgccct cgtcggcggc agcgcggccg ccccgtcccg gctgaccccc gacgactacg
     2221 tcggcaactc gtccgaccgg accggcttcg ccggactcga ggccgtcgac gaggtcacca
     2281 tgctgtccgt gcccgacctg atggccgtgt acgagcaggg catgatcgat ctggagggcc
     2341 tccaggccgt ccagctcgcg atgatcgccc actgcgagct gatgggcgat cgcgtcgcca
     2401 tcctcgacgc gccgccaggg ctcaacgccc agcaggtcaa ggagtggcgg gtcgacaagg
     2461 ccggctacga cagcaagtac gcgaccctct actggccctg gatcaaggtc ttcgaccccc
     2521 tgtccggcca ggcgaagttc gtgccgccgt ccggccacac ggccgggatc tgggcccgca
     2581 acgacgacac ccgcggcgtc cacaaggcgc cggccaacga ggtcgttcgg ggcgcgatca
     2641 gcctcgcgct caacctgacc aaggccgagc acgaccagct caaccccaac ggaatcaact
     2701 gcatccgggc cttccccggt cgcggcatcc gggtgtgggg cgcgcggacg atctcgagcg
     2761 atccgtcctg gcgctacctc aacgtgcggc gcctgttcaa ctacatcgag gagtccatct
     2821 tcgagggcac ccagtgggtg gtcttcgagc ccaacgacca gcgcctgtgg ggccgcgtga
     2881 agcggaccat caacgcgttc ctgctgcggg tctggcgtga cggcgcgctg ttcggcgcga
     2941 cgcccgacca ggcgttcttc gtcaagtgcg acgaggagaa caacccgccc gaggtccgcg
     3001 atgccggcca gctgatcatc gacatcggca tcgcgcccgt gaagccggcc gagttcgtgg
     3061 tgttccggat cgcccagttc tctggctcgc cggacgccga gtagccgtca tgaccacgca
     3121 aacggaaggc cacggaggct cgcatgccca cgactgatcc gctgctgcaa ggcaacgtcc
     3181 tgctggacat cgacggcgtc ggcgtcgtcg ccaacttcac ctcggtctcg ggcgtctcga
     3241 gcgaggtgga gatcgtcgac aacccgttcg tcaacgacca gggcaaggtc accatcggca
     3301 agctcccggg caagccgaag accccgacgg tcaccctcaa gcggggcctg accggggcga
     3361 ccgagatctg ggactggcac accgccgtca tgcaggggaa cccggaccgt cgcaacggct
     3421 cggtgatcct catgaacacc atcggcgagg agatcctgcg ctacaacttc accaacgcga
     3481 tggtgtccaa gatcagcctc tccgagggcg gcgccacgac cagcggcgtc cagatcgagg
     3541 aggcgaccct ggcctgcgag acgctcgtca agggctaggt cgatgaccac cgcactcctg
     3601 cccgagctcg gtgccgagcg ccttggcacg aacgcgtccg acgccctccg gacggaatac
     3661 gcgttcgtgc tgccgcgcgg cttcgtcgac gggtccggca ccgtccatcg cgacggcgtc
     3721 atgcgcctcg cgacggcgcg cgacgagatc atcccccagc gggatcctcg cgtccgcgag
     3781 aacgaggcct acctgacgat cctgctgctg tcccgggtcg tgacccgact cgggacgctg
     3841 ccccaggtgc atacaggggt catcgagggc ctgttcgcat cggacctcgc cttcctgcag
     3901 gacctctacc gccgcgtcaa ccaggagggc cacacgcagg cgtcggtgac gtgtcccgcg
     3961 tgcaatcacg cgttcacggt cgacgtggcg ggtgagcagc gcctggggga atcgtgacgt
     4021 acgcgactga ccgcctgttt gaagaggtcg cgtacgtcgc ctaccacttc cactggccga
     4081 tggacaccat cctcgacctc gaacacccgc tgcggctgcg gttcgtggat gagatcggcc
     4141 gcatcaatcg gcgcctgtcc gaggaggagt gagtcccgtg cgtctgccgt ggccgttcgg
     4201 ccgcagcgcg tccgacgacg gggcgtcctc gacctccggt gacgccggtg ccggcgtcac
     4261 cgacgcggtg ccggcctccc cgcctgtccc cgcgccgatc ccgcccacgg gagcctggcg
     4321 aacgctgccg cccatccagc gaacggcagg tccgccgccg gtggtcgcgc ccgccgcgcc
     4381 gttcctggcg gccgtgcccg gccatgcccc gctgccgccg atcgtgagcc cgctgggcca
     4441 cgagtcgagt ccggtcgcgc cggcgggcat cgtcgtcgcg cacccccacg ccgtcccgag
     4501 cctgacgtcc ggtgcgccgc tgacgacgag gcaggtgcag cgacacccct ccgccgacag
     4561 ccctgccgcg gacagcccgg cggccgccgc cgacgaggga tcgcccgtcg tcgcgcgcgc
     4621 ggtcgacgcg cccgtcgtcg cggaggtgcg caccgtgacg ccgccgcccg aactcgacct
     4681 gccgccgatc cggacggtct cgccggtgac gccggccgcg accgtcacgc cgagcccgag
     4741 gccgctcacc caggcctcgc cgacgct
//
LOCUS       DBNHXO010000470        16262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3920624, whole genome shotgun sequence.
ACCESSION   DBNHXO010000470 DBNHXO010000000
VERSION     DBNHXO010000470.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 16262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16262
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3920624"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="AC162A_09525"
     CDS             complement(<1..520)
                     /locus_tag="AC162A_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HIR5011124.1"
                     /translation="MCGVVGVVLPDRGHEAAGVAATALFALQHRGQESAGVGVSDGRH
                     LMIYKDLGMVGQVLDERRIPSLAGDLAVAHCRYSTTGSTVWENAQPTLRLGPRRALAI
                     GHNGNLVNTRQLLDQLEGGRGRLPASTDTELLTALLADEPAEDTVDALRRVLPRVKGA
                     FSLVILSEHQVIG"
     gene            complement(520..2880)
                     /gene="purL"
                     /locus_tag="AC162A_09530"
     CDS             complement(520..2880)
                     /gene="purL"
                     /locus_tag="AC162A_09530"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HIR5011125.1"
                     /translation="MTATAAAPLHRVLGLEDVELEAIREKLGRDPNDLELAMFSVMWS
                     EHCSYKSSRPLLATLPTAGRDVVAGVGEQAGVISIGDGLAVAFKIESHNHPSAVEPYQ
                     GAATGVGGILRDVFAMGARPIAVLDALRFGDPSDPRTRHLVDGVVRGVGGYGNCVGVP
                     TVGGELVFDPSYQGNPLVNVMAIGLLEEGMLTRASADGVGNLVVLYGSATGRDGIGGA
                     SVLASATFGDQDPSKRPSVQVGDPFAEKLLIEATLELIAAGLVVGVQDLGAAGITCAT
                     CETADRGGTGMLVDLDAIPRREPGLEPFEVMISESQERMVAIVEPARWEAVRAVCERW
                     DLPVAIIGRVTAEPDIVVLTGPGGEGALGGDGRPVEGARELARIPAAALASGAIVFQR
                     ESRPPARRRSAPAPGAPVEPIDTLPLRGQDPGAVLQALLGSPNLSSRRSVYEQYDHNV
                     QANTVAGPGRGAAVLRIKGTTKALVASTDANAPVGALDPWLGAALSVAEAARNVAITG
                     ARPLGVTNCLNFGDPTRPEAFWQLTEAVRGLSDACVALGLPVTGGNVSLYNEAPGSAI
                     YPTPEIGVVGLLEDVGKRVGPAFRGAGNAVLLVGQAGAGLAGSEYARLAGAAVEDGPP
                     ALDLAREERLQSFIREAVDRGLVESCQDVSGGGLAVAVAEMAMWGDVGARLRLTVGDS
                     PAVGLFGESPSRLIVEVAPRYVPAFILLARHYGLPGDELGMTGGSRLVIELAGEGATG
                     AAEERGSRIADALDVAVADLRHAWDHGLPRALGWAAAATDAKAADA"
     gene            complement(2877..3650)
                     /gene="purQ"
                     /locus_tag="AC162A_09535"
     CDS             complement(2877..3650)
                     /gene="purQ"
                     /locus_tag="AC162A_09535"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIR5011126.1"
                     /translation="MTARIGIVLFPGSNRDIDAVNSVTIAGAEPVMLWHEAADLDGVA
                     GVLLPGGFAYGDYLRAGVIARFSPVMRAVADFASRGGPVLGICNGFQVLAEAGLVPGA
                     LLRNRGLRFVCREVTLLPEHLDSPFTRELDGSPLRMPVAHGEGCYYADEPALDDLERD
                     GRVLWRYASADGSVALPDDRGNPNGSLRGIAGVRNAAGNVAGLMPHPETASEAILGSD
                     DGLGIIRSFVVSAAEWAATGSITGLAASAAEPVTAGAAR"
     gene            complement(3647..3949)
                     /gene="purS"
                     /locus_tag="AC162A_09540"
     CDS             complement(3647..3949)
                     /gene="purS"
                     /locus_tag="AC162A_09540"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HIR5011127.1"
                     /translation="MSDPTRAFRFAVNVTPKPGILDPQGRAVERSLPHLGIEGVGDVR
                     VGRRVELTVTAADEASARAVVERLAGELLSNPLIEAYEVEAVGEARSVATGAGAAG"
     gene            complement(3946..4863)
                     /locus_tag="AC162A_09545"
     CDS             complement(3946..4863)
                     /locus_tag="AC162A_09545"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR5011128.1"
                     /translation="MRLAEVFVRAGKVRDLYRIGDDRLLLVASDRLSAYDVVLPTPIP
                     DKGRVLTGLSRFWFAQTSSIVPNHLLSTDPADVPAEITDGDPDIVDTLRGRMMIGRLA
                     EVLPIECVVRGYLSGSGWKAYLDDGAVCGIPLPPGLRESDRLPAPIFTPATKAPMGEH
                     DENIPFESVETLIGPALAAAIRETSIALYRHGAEVCERAGIILADTKFEFGVLPSGEL
                     LLIDEVMTPDSSRFWDAAAYRPGGPQPSFDKQYVRDWLVGQAWDKTPPGPELPLDVVA
                     GTRARYVAAFELITGASFDRYLQEDVIAR"
     gene            complement(4850..6169)
                     /gene="purB"
                     /locus_tag="AC162A_09550"
     CDS             complement(4850..6169)
                     /gene="purB"
                     /locus_tag="AC162A_09550"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012623747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR5011129.1"
                     /translation="MIPRYTLPEMGAVWTDRARFERMLEVEIAVCKAQVGRGLVPADA
                     LTAIESRAKVDVDRIAEIERTTDHDVIAFVSQVAEAVGPEGRYLHLGLTSSDVVDTAL
                     ALQLQAAGTRLLDDADRLTAALVARARAEARTVMMGRTHSVHAEPTTFGLKCAGWAFE
                     VARGRTRLAEATAEIGTGKISGPVGTYSHLAPDIEDEVLTSLGLRVDPVSTQVVQRDR
                     HAAFLAAIAIIGGTLERLATEVRNLQHTEIAEVMEPFRAGQKGSSAMPHKRNPILSER
                     IAGLARLLRGYAQTGLENQPLWHERDISHSSAERVALPDATILLDYMLVRATGLVEGL
                     VVRPERMRENIERGLGLHASSRVLLALVERGGLSREDAYAIVQRNALKAADERVPLQK
                     LLATDPQVAQKLPLAELDACFDDTALLRHVPEVIARLDRLEVARAAR"
     gene            complement(6166..7494)
                     /gene="purD"
                     /locus_tag="AC162A_09555"
     CDS             complement(6166..7494)
                     /gene="purD"
                     /locus_tag="AC162A_09555"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004005520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR5011130.1"
                     /translation="MTLVMPTRILVVGGGGREHALAWKLGREPGVNEVLVAPGSAGIA
                     GEPHVRVAGLDPLEPVAVVALAREHAAELVVVGPEAPLATGVADALREAGVAVFGPDR
                     KAARLETSKAFCHEVAEAAGVRTPRARAFRPGEVATAEAFVRELDATGAGAVLKADGL
                     AGGKGVIVTESLGQALELVPSFLAGRPADEPALVIEERLQGREASVIALCDGTSAIAL
                     PAARDHKRLCDDDRGPNTGGMGAYSPLPDLDANAVERVLEAVHRPILAEMAGRGSPFR
                     GFLYAGLMLTADGPALLECNVRLGDPEAQVILPRIAGSLGPLLLAAARGRLPVDAPPR
                     IPELPGAAVGIVLAAKGYPGDPRRGDPIRGLETATGPGALVFHAGTLGRPQGGFGTNG
                     GRVLTVVGRGPDLTAARDAAERAADTIDWDGLQRRHDIARDLPIPAGARA"
     gene            complement(7513..8820)
                     /locus_tag="AC162A_09560"
     CDS             complement(7513..8820)
                     /locus_tag="AC162A_09560"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR5011131.1"
                     /translation="MPATVIVGLQWGDEGKGKTTDFLAEHVAVVVRYQGGDNAGHTVV
                     VGDETVKLQLCPSGVMYPHITSVIGNGVVVNPATLIAELDMLKARGIDVSKVRVSRSA
                     HVIMPYHVALDKANEVRLGGAKVGTTGRGIGPAYGDRAWRLGLRMEDLCDREVLRERI
                     GRALPDKNLLLGSMGAEGFTVDALVDQASAWGERLAPHLDDTTWLVQDALRRGDHVLL
                     EGAQGTLLDLDHGSYPYVTSSNPVAGGACTGGGIGPLQVDEVIGVMKAYSTRVGSGPF
                     PTELFDDIGKGIAERGREFGTVTGRPRRVGWFDAVPMRYAVAVNSNTAVVLNKLDILS
                     GIETIRLCVAYEIDGRRVDVWPSSGSALSRATPIYEAFEGWEQPIHDVRSLADLPEAA
                     RRYVSALEEHAGVPIVFVSVGPERTQTIERAWRPKRHRPTPAA"
     gene            complement(9072..9221)
                     /locus_tag="AC162A_09565"
     CDS             complement(9072..9221)
                     /locus_tag="AC162A_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011132.1"
                     /translation="MRPDGHDIAARRDVIVVGAGDIGLATAREVARRGNAVLAIDRFG
                     SGLSR"
     gene            9751..11046
                     /locus_tag="AC162A_09570"
     CDS             9751..11046
                     /locus_tag="AC162A_09570"
                     /inference="COORDINATES: protein motif:HMM:NF024799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR5011133.1"
                     /translation="MRTTVPFTALAVISIVVAACSSSAATPSPTTAPPPSPSASEAPS
                     AGASGSEAGSPASSAAAVSIDDFDSSFSAMATLAPVTSAGSGLVGVILPDVTTSARYT
                     SYDLPYLKQAFEAAGYPATDFKIDNASGTATTELALAQADITAGAKVLLVDPLDSTTG
                     NQIQSAAAAAGVPMISYDRATFQGTDTYYVSFDNQQVGKLIGQGLMECVSDWGVTSPQ
                     VFTLDGGQDTDPNAVDFAKGYNSAIWGQPVAQVAKGATNSAGYTLVNEQIAPGWDNKA
                     GGTIFQQAYTADPSINATVEANDGLAGAVIGVLKAAGVPAKKVPTTGQDATLQGMEFV
                     LQGYQCGSVYKPIYLEAQAAAALATYLRAGQTPPAGLINGTTIDPANSANTQPAVLLT
                     PSWVNATNMESTVIKDNFVSATDLCAAVGASVCTAAGIQ"
     gene            11140..11955
                     /locus_tag="AC162A_09575"
     CDS             11140..11955
                     /locus_tag="AC162A_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013473651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5011134.1"
                     /translation="MTTVAPPAGATADASAPLLALRGISKHFGAVQALYQVDLDLPSR
                     QVTALCGDNGAGKSVLTRSIAGIHQVDTGEIWWEGRPVHIRNPSDAAALGIETVYQDL
                     ALADNLDIVQNMFLGRERLVHGLLDEDAMERAAAETLASLRVTTVRSIRQPVATLSGG
                     QRQAVAVAKAVMWNSKLVMLDEPTAALGVAQTRMVLDLILQLKERGLAVMLISHNLND
                     VFDVADRIAILRLGRMVAQDAIGAFDRQSVVDFMTTGRSQRGLTAKAVGHGPH"
     gene            11942..13276
                     /locus_tag="AC162A_09580"
     CDS             11942..13276
                     /locus_tag="AC162A_09580"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIR5011135.1"
                     /translation="MDRTESLPPVGPAPGVDAAPDLTAAVDLPTPVGAQTVGEYRRAW
                     VARVRGGDAGVLPVAIALVAVVTVFTVVSPNHVFLSARNLVNLFDQSAVFIVLAMGEG
                     FVLLLGEIDLSIGYVAAIGGIVAALLVQPSPGWPWPAAIVAALVVTGAIGGIQGTIIT
                     RLGMPSFVVTLAGYLIWFGVMIVILGTSGQVGITSTVLPGQEALYGIVYSYLSPVAGW
                     VGLIVVVGLYGIVTWRRDARRRRSGLVAPPVGLTVARIAAAAAAGVVVVAICNVDRGS
                     FLSVVGVPWVVPLVLAVLVLWSMLLERTQYGRHVYAVGGNAEAARRAGVGVPRVRTVA
                     FVLCSMTAGIAGIIYCSQLGGMTTNINGGQLVLYAVAAAVIGGTSLYGGRGRVVHGLL
                     GGLVIGAIYNGLYLLGLPIQYQLIVTGLVLLAAVSVDSLSRRGAGTGSAYPT"
     gene            13523..13969
                     /locus_tag="AC162A_09585"
     CDS             13523..13969
                     /locus_tag="AC162A_09585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR5011136.1"
                     /translation="MNRVLITGRLTRDPELRTTAGGKAVAQFAVASHAYVSGKETSEF
                     HNVVAWDRLAETCGRYLGKGQLVAVEGRIQTRSWDDDKGVRHWKTEIVASSVEMLSGR
                     RKKDYAAETAATAMDAQAAAEGFAPDAGEPVADEAGFSVDPGGDDV"
     gene            complement(14075..14941)
                     /gene="rsmI"
                     /locus_tag="AC162A_09590"
     CDS             complement(14075..14941)
                     /gene="rsmI"
                     /locus_tag="AC162A_09590"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR5011137.1"
                     /translation="MTAASTGDAARGGTLYVVATPIGNLGDVTLRALEVLRAVPLIAA
                     EDTRISRRLLDRYEISTRAVSYHARSGPARERELLAHLEGGANLALVTDAGTPVVSDP
                     GEGLVAAWAAAGGSVVPIPGASSVLAAVAASGVAGPRWSFEGFLPRSGRERRQRLARI
                     AAEERGTGQFEAPGRMAATLRDLAASCGAERPGAVCRELTKLHEQIVRGSLGELAALA
                     ADGEIRARGEIVIVVGWGSAAEADGDDAEAALAAARARVDELVAAGSPRGDAARTVAA
                     ATGLPRRALYVP"
     gene            complement(14944..15837)
                     /locus_tag="AC162A_09595"
     CDS             complement(14944..15837)
                     /locus_tag="AC162A_09595"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR5011138.1"
                     /translation="MTEGAALFVLPDELAIDTGVARRVVAEFIRGQLSQAGFDRAVLG
                     LSGGIDSALVAYLVAETIGADKLLCVLMPYRTSSPDSRADAEEVVRRLGCASELVDIS
                     PMVDGYYGADGVAGAAGDEGLAASPLRRGNLMARMRMSVLYDRSVVWGGLVVGTGNKT
                     EALIGYTTLFGDSACAFNPVGDLYKSQVRQVSADIGVPEAIIRKAPSADLWPGQTDET
                     EAGFTYHELDRLLFWRIDKRRSVEEMVAMGFDRATVERVDRMVAGAEFKRQVPPIAKL
                     GPRTAGVDYLYPRRRPGSARP"
     gene            complement(15834..>16262)
                     /locus_tag="AC162A_09600"
     CDS             complement(15834..>16262)
                     /locus_tag="AC162A_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR5011139.1"
                     /translation="SPGRDLARSSEVGLGTANSWRTLMRTYAQLTTSFVVFCNRVGVD
                     ESISFWGGSEVIGPGGAPIFSAPLFDEGLFFADIDLGDVRRERIALPLLRDERPELVA
                     REWRRLIAERSGLAADGNAEPGSQDGFDVAVEPARTSSDR"
BASE COUNT         2268 a         6080 c         5790 g         2124 t
ORIGIN      
        1 cgccgatgac ctggtgctcg gagaggatca cgaggctgaa cgcgcccttg acccggggca
       61 ggacccggcg aagcgcgtcg acggtgtcct cggccggctc gtcggcgagc agcgcggtca
      121 ggagctccgt gtcggtgctg gcgggaaggc gcccgcggcc gccctccagc tggtcgagga
      181 gctgccgggt gttgacgagg ttgccgttgt gcccgatggc cagcgcgcgg cgcggcccga
      241 ggcggagcgt cggctgggcg ttctcccaga cggtcgaccc ggtggtcgag taccggcagt
      301 gggccacggc gaggtcgccg gcgagtgacg ggatccgccg ctcatcgagc acctggccga
      361 ccatgcccag gtccttgtag atcatcaggt gccgcccgtc ggacaccccg acgccggccg
      421 actcctggcc gcggtgctgg agcgcgaaca gggcggtggc cgcaaccccg gccgcctcgt
      481 gaccacgatc gggcaggacg acgccgacga cgccacacat caggcgtcgg ccgcctttgc
      541 atccgtggca gcggccgccc agccgagcgc ccgcggcagc ccgtggtccc aggcgtggcg
      601 caggtcggcg acggcgacgt cgagggcgtc ggcgatccgg ctcccccgtt cctcggcggc
      661 cccggtggcg ccctcgcccg cgagctcgat gaccagccgc gatcccccgg tcatgccgag
      721 ctcgtcgccg ggcagcccgt agtggcgggc cagcaggatg aacgcgggca cgtagcgtgg
      781 cgcgacctcg acgatcaggc gcgatgggct ctcgccgaac aggcccaccg ccggcgagtc
      841 gccgaccgtg aggcgcagcc gggcgccgac gtcgccccac atcgccatct cggccaccgc
      901 gacggcgagc ccgccgcccg acacgtcctg gcaactctcg acgaggcccc ggtccacggc
      961 ctcgcggatg aacgactgga ggcgctcctc gcgggcgagg tcgagcgcgg gcgggccgtc
     1021 ctcgaccgcg gcgccggcca gccgcgcgta ctcgctgccc gcgaggccgg ctcccgcctg
     1081 ccccaccagc agcaccgcgt tccccgcgcc gcggaacgcc gggccgacgc gcttgccgac
     1141 gtcctcgagc aggcccacca cgccgatctc cggggtcggg taaatcgcgg accccggcgc
     1201 ctcgttgtac agcgagacat tgccgccggt gaccggcagt ccgagcgcca cgcaggcgtc
     1261 ggagagcccg cggaccgcct cggtgagctg ccagaaggcc tccggccggg tggggtcgcc
     1321 gaagttcagg cagttcgtga ccccgagcgg acgggctccc gtgatcgcga cgttgcgcgc
     1381 ggcctcggcg accgacagcg cggcgcccag ccagggatcg agcgcgccaa ccggggcgtt
     1441 ggcgtcggtc gaggcgacca gcgccttggt cgtgcccttg atccgcagca ccgcggcgcc
     1501 gcgaccgggt ccggcgaccg tgttcgcctg gacgttgtgg tcgtactgct cgtagacgct
     1561 gcgacgcgac gagaggttgg gcgagccgag cagcgcctgg agcaccgcgc ccggatcctg
     1621 gccgcggagg ggcagggtgt cgatcggctc gacgggggcg ccgggggcgg gcgccgagcg
     1681 acgccgggcg ggcggtcgcg actcgcgctg gaacacgatc gcgcccgatg cgagggccgc
     1741 ggccgggatg cgggcgagct cgcgggcacc ctcgacaggg cggccgtcgc cacccagcgc
     1801 accctcgcca cccgggccgg tcagcacgac gatgtcgggc tcggcggtga cccgcccgat
     1861 gatcgcgacc ggcaggtccc agcgctcgca cacggcgcgc acggcctccc agcgcgcggg
     1921 ctcgacgatc gcgaccattc gctcctggga ctccgagatc atcacctcga acggctcgag
     1981 gccgggctcg cggcgcggga tcgcgtcgag gtccacgagc atcccggtcc ccccgcgatc
     2041 ggcggtctcg caggtcgcgc aggtgatccc ggccgcgccc aggtcctgga cgccgaccac
     2101 gagacccgcg gcgatcagct cgagggtcgc ctcgatcagg agcttctcgg cgaacgggtc
     2161 cccgacctgc acggaggggc gcttcgacgg gtcctgatcg ccgaaggtcg cgctggccag
     2221 caccgacgcc ccgccgatcc cgtcgcgacc cgtcgccgag ccgtacagga ccacgaggtt
     2281 cccgacgccg tccgcggacg cgcgggtcag cattccttcc tcgagcagcc cgatcgccat
     2341 cacgttgacc agcgggttgc cctggtacga ggggtcgaac acgagctcgc cgcccaccgt
     2401 cggcacgccg acgcagttgc cgtacccgcc gacgcctcgg acgacgccgt cgacgaggtg
     2461 ccgggtgcgc ggatcggacg ggtcgccaaa ccgcagcgcg tcgaggaccg cgatcggccg
     2521 cgcgcccatc gcgaacacgt cgcgcaggat cccgccgacg cccgtcgcgg cgccctggta
     2581 cggctcgacc gccgacgggt ggttgtggga ctcgatcttg aacgcgaccg ccagcccgtc
     2641 gccgatcgag atcacacccg cctgctcccc cacgccggcc acgacgtcgc ggcccgcggt
     2701 cggcagcgtc gccagcagcg gccttgagct cttgtacgag cagtgctcgc tccacatcac
     2761 gctgaacatg gcgagctcga ggtcgttcgg gtcgcggccc agcttctcgc ggatggcctc
     2821 gagctcgacg tcctcgaggc ccaggacccg gtgcagcggg gcggcggcgg tcgccgtcaa
     2881 cgcgccgccc ccgcggtgac gggctcggct gcggatgccg cgagccccgt gatcgacccg
     2941 gtcgccgccc actcggcggc gctgaccacg aacgagcgga tgatcccgag gccgtcgtcg
     3001 gaaccgagga tggcctcgga ggcggtctcg gggtgcggca tgagccccgc gacgttgccg
     3061 gccgcgttgc gcacgcccgc gattccgcgc agcgagccgt tggggttgcc ccggtcgtcg
     3121 ggcagggcca ccgacccgtc ggcggacgcg tagcgccaca ggacgcgccc gtcgcgctcc
     3181 aggtcgtcga gcgcgggctc gtcggcgtag tagcagccct cgccgtgcgc gaccggcatc
     3241 cgcagcgggc tcccgtcgag ttcgcgcgtg aacggcgagt cgagatgctc ggggagcagc
     3301 gtgacctcgc ggcagacgaa gcggagcccg cggttccgaa ggagcgcgcc gggcacgagc
     3361 cccgcctccg ccagcacctg gaacccgttg cagatcccga gcaccggccc gccgcggctc
     3421 gcgaagtcgg cgaccgcgcg catgaccggg ctgaagcgtg cgatgacgcc ggcccggagg
     3481 tagtcgccgt acgcgaaccc gcccggcagc agcacccccg cgacgccgtc gaggtcggcc
     3541 gcctcgtgcc acagcatcac gggctcggcg cccgcgatcg tgacggagtt gacggcgtcg
     3601 atgtcccggt tggagccggg gaacagcacg atgccgatgc gggcggtcac ccggccgccc
     3661 ccgcgccggt ggccaccgac cgtgcctcgc ccaccgcctc gacctcgtag gcctcgatca
     3721 acgggttcga cagcagctcg cccgccagcc gctcgaccac cgcccgcgcc gacgcctcgt
     3781 cggccgcggt gaccgtcagc tcgacccgcc gcccgacgcg gacatcgccg accccctcga
     3841 tcccgaggtg cggcaggctg cgctcgacgg cgcggccctg cgggtcgagg atccccggct
     3901 tgggcgtcac gttgacggcg aatcggaagg cgcgggtggg gtcgctcatc gggcgatgac
     3961 atcctcctgg aggtagcggt cgaagctggc gccggtgatc agctcgaaag cggcgacata
     4021 gcgggcgcgt gtgccagcga cgacgtcgag cggcagctcg gggccgggcg gcgtcttgtc
     4081 ccacgcctgg ccgacgagcc agtcgcggac gtactgcttg tcgaagctcg gctggggccc
     4141 gcccgggcgg taggccgccg cgtcccagaa gcgcgacgag tcgggcgtca tgacctcgtc
     4201 gatcagcagc agctcgcccg aggggagcac gccgaactcg aacttggtgt ccgcgaggat
     4261 gatcccagcc cgctcgcaga cctccgcgcc gtgacggtag agcgcgatcg aggtctcgcg
     4321 aatagccgcc gcgagcgcag gcccgatcag cgtctcgaca ctctcgaacg ggatgttctc
     4381 gtcgtgctcg cccatcggcg ccttcgtcgc gggcgtgaag atcggggccg ggagccggtc
     4441 gctctcgcgc aggcccgggg gcagcgggat cccgcacacc gccccgtcgt cgaggtacgc
     4501 cttccagccc gatcccgaca ggtagccgcg gacgacgcac tcgatcggca gcacctcggc
     4561 cagccgcccg atcatcatcc ggccgcggag cgtgtccacg atgtccgggt cgccgtcggt
     4621 gatctccgcg gggacgtccg ccgggtcggt cgagagcagg tggttcggga cgattgagga
     4681 ggtctgggcg aaccagaacc gcgacaggcc ggtcagcacg cgccccttgt cgggaatggg
     4741 cgtcggcagg acgacgtcgt acgccgacag ccggtccgag gcaacgagca gcagccggtc
     4801 gtcgccgatg cggtacaggt cccgcacctt gccggcgcgg acgaagacct cagcgagccg
     4861 cacgggccac ctccaggcgg tcgaggcggg cgatgacctc ggggacgtgg cgcagcagcg
     4921 ccgtgtcgtc gaagcaggcg tcgagctcgg ccagcggcag cttctgcgcg acctgcggat
     4981 cggtcgccag gagcttctgc agcggcaccc gctcgtccgc ggccttgagg gcgttgcgct
     5041 ggacgatggc gtacgcgtcc tcacgcgaca acccaccccg ttccaccagg gccaggagca
     5101 cccgggacga cgcgtggagc cccaggccgc gctcgatgtt ctcgcgcatg cgctccgggc
     5161 ggacgactag gccctcgacc aggcccgtcg cgcggacgag catgtagtcg agcaggatcg
     5221 tggcgtcggg cagcgcgacg cgctcggcgg agctatggct gatgtcgcgc tcgtgccaca
     5281 gcggctggtt ctcgagcccg gtctgggcgt agccgcggag cagccgcgcg agccccgcga
     5341 tccgctccga gaggatcggg ttgcgcttgt ggggcatcgc cgagctgccc ttctggcccg
     5401 cccggaacgg ttccatgacc tcggcgatct cggtgtgctg gaggttgcgg acctcggtcg
     5461 cgaggcgctc gagggtgccg ccgatgatgg cgatggccgc gaggaaggcc gcgtggcggt
     5521 cgcgctggac gacctgtgtg ctcacgggat cgacccgcag cccgagcgac gtcaggacct
     5581 cgtcctcgat gtccggcgcg aggtggctgt acgtcccgac ggggccgctg atcttgccgg
     5641 tcccgatctc ggccgtcgcc tcggccaggc gggtccggcc gcgcgcgacc tcgaaggccc
     5701 agccggcgca cttgagcccg aacgtggtcg gctcggcgtg gacggagtgg gtccgaccca
     5761 tcatcaccgt gcgggcctcg gcacgggcgc gggcgacaag ggcggccgtg agccgatcgg
     5821 cgtcgtcgag gaggcgggtg ccggccgcct ggagctgcag ggcgagcgct gtgtcgacga
     5881 cgtcgctgct ggtcaggccg aggtggaggt agcggccctc cggacccacc gcctcggcga
     5941 cctgcgagac gaaggcgatg acgtcgtggt cggtggtgcg ctcgatctcg gcgatccggt
     6001 cgacgtcgac cttcgccctc gactcgatcg cggtcagcgc gtccgccggc acgaggccac
     6061 gcccgacctg ggccttgcag acggcgatct cgacctcgag catgcgctcg aagcgggccc
     6121 ggtcggtcca cacggcgccc atctcgggca gcgtgtagcg cgggatcatg ccctcgctcc
     6181 cgcggggatc ggcaggtccc tcgcgatgtc gtggcggcgc tgcaggccgt cccagtcgat
     6241 cgtgtccgcg gcccgctcgg cggcgtcgcg ggcggccgtc agatccggtc cgcgaccgac
     6301 caccgtcagc acccgcccgc cgttcgtccc gaagccgccc tgcggccggc cgagcgtccc
     6361 ggcgtggaag acgagcgcac ccggcccggt cgccgtctcg agccccctga tcgggtcgcc
     6421 ccggcgcggg tcgccgggat agcccttcgc agccagcacg atgcccacgg cggccccggg
     6481 cagctcaggg atgcggggcg gggcgtcgac cggcagccgg ccgcgggcgg cggcgaggag
     6541 cagcggcccg agggagccgg cgatccgcgg caggatcacc tgggcctccg ggtcgccgag
     6601 ccggacgttg cattcgagaa gggccgggcc gtcggcggtg agcatcagcc cggcatagag
     6661 gaagccacgg aacggtgatc cgcggccggc catctcggcc aggatcggcc ggtggaccgc
     6721 ctcgagcacg cgctcgaccg cgttggcgtc gaggtcgggc agtggcgaat aggcgcccat
     6781 gccgcccgtg ttcggccccc ggtcgtcgtc gcacagccgc ttgtggtcgc gggctgcggg
     6841 aagggcgatc gcgctcgtcc cgtcgcacag cgcgatcacg ctcgcttccc tgccctggag
     6901 ccgctcctcg atcacgagcg cgggttcgtc ggccggccgc ccggcgagga acgaaggcac
     6961 gagctccagc gcctgcccga gcgactcggt gacgatgacg cccttgccgc cggccaggcc
     7021 gtcggccttg aggacggcgc cggcccccgt cgcgtccagc tcacggacga aggcctcggc
     7081 cgtcgcgacc tcgcccggtc ggaacgcgcg ggctcgcggc gtccggacgc cggccgcttc
     7141 cgccacctcg tggcagaacg ccttcgatgt ctcgagcctc gccgccttgc gatccggccc
     7201 gaacacggcg acccccgctt cacgcagggc atcggcgacc ccggtcgcca gcggcgcctc
     7261 cggcccgacc acgaccagct cggccgcgtg ctcgcgcgcg agcgcgacga cggccaccgg
     7321 ttcgagcgga tcgaggccgg caacacggac gtggggctcg ccggcgattc cggcgctgcc
     7381 gggcgcgacc aggacctcgt tcacccccgg ctcccggccg agcttccacg ccagcgcgtg
     7441 ctcacggccg ccaccgccga cgacgaggat ccgggtgggc atcacgagcg tcatcggtgg
     7501 ctgctcatcg ggtcaggccg ccggcgtcgg gcgatggcgc tttggccgcc acgcgcgctc
     7561 gatcgtctgg gtccgctccg ggccgacgga gacgaacacg atcggcacgc cggcatgctc
     7621 ctcgagcgcg gacacgtagc ggcgggcggc ctcgggcagg tcggcgaggc tgcggacatc
     7681 gtggatgggc tgctcccacc cctcgaacgc ctcgtagatc ggggtcgcgc gcgagagcgc
     7741 cgatccgctc gagggccaga cgtcgacccg cctgccgtcg atctcgtagg cgacgcacag
     7801 gcggatggtc tcgatgcccg acaggatgtc gagcttgttg agcacgacgg cggtgttcga
     7861 gttcaccgcg acggcgtagc gcatcggcac ggcgtcgaac cagccgacgc gacgcggccg
     7921 cccggtgacg gtcccgaact ctcgcccccg ctcggcgatg cccttgccga tgtcgtcgaa
     7981 cagctcggtc gggaacggcc cggaaccgac cctcgtcgag taggccttca tgaccccgat
     8041 gacctcgtcg acctggagcg gcccgatccc gccgcccgtg caggcgccgc cggcgaccgg
     8101 gttcgaggag gtcacgtacg ggtagctgcc gtgatcgagg tcgaggagcg tgccctgagc
     8161 accctcgagc agcacgtggt cgccgcgacg gagcgcgtcc tggacgagcc aggtggtgtc
     8221 gtccaggtgt ggcgcgagcc gctcgcccca cgctgatgcc tggtcgacga gcgcgtccac
     8281 cgtgaagccc tcggcgccca tcgagccgag cagcaggttc ttgtcgggca gggcacgtcc
     8341 gatgcgctcg cgcagcacct cgcggtcgca caggtcctcc atccgcaagc cgaggcgcca
     8401 ggcccggtca ccgtacgccg gcccgatccc acgcccggtg gtgcccacct tggccccgcc
     8461 gaggcggacc tcgttcgcct tgtcgagcgc cacgtggtac ggcatgatca cgtgcgcgct
     8521 gcggctgacg cggaccttcg acacgtcgat gcccctcgcc ttgagcatgt cgagctcggc
     8581 gatcagcgtt gccggattga ccacgacacc gttgccgatg accgacgtga tgtgcgggta
     8641 catcacgccc gaggggcaca gctggagctt gaccgtctcg tcgcccacga cgaccgtgtg
     8701 gcccgcgttg tcgccaccct ggtagcggac gacgacggcc acgtgctcgg ccaggaagtc
     8761 ggtcgtcttg cccttgccct cgtcgcccca ctggaggccg acgatcaccg tcgccggcat
     8821 cagcggatcc ccggcacgaa agaaccccgg tcggggccgg ggtcctggat acacgtctcc
     8881 cgcgacagcc cgcgggcgtt ccgacgggcc ggggggtcga aggtgcgagc ccgtctcacg
     8941 gggcggacgc cgcgcctccc tcgggcaatg gtgatctcct ccatcgcggg actggccccg
     9001 cggggcctcg aggacagcgc cgagtatagc ggcccctgac cgcccgcggt tcggcccgtg
     9061 ggccgtcggg ctcagcggga gaggccgctg ccgaagcggt cgatggcgag caccgcgttg
     9121 cctcgacggg cgacctcgcg cgcggtggcc aacccgatgt cgcccgcgcc cacgacgatg
     9181 acgtccctgc gcgccgcgat gtcgtgtcca tcgggcctca tcgtacggac gaccggcggg
     9241 catttcgccc ggtgcgatgc ggttggaccg atctcagcga aatctcacga acggcgcgca
     9301 ggttgcagat cacggagggc cacgacacgc gggcccccac aacgccgctc tcaccggttc
     9361 gctggttgga gagccgcccc cgaacagggg cgacccgaca ccgacggccg tcccggccga
     9421 tgtctcaccg gctccgatcg accgccgatc cagcgcccgt cgcgcacaag gagtcgatct
     9481 cgccccgctc cggccgccca cgcacagacc gcgtggcgac ggccgccacc tcgcggaatc
     9541 gtcgactcgc gaacgatcgg gcttgccgcg ctgagcacgg gtccatcggc gtgtcgcaat
     9601 cgggaggagc gcggtctccc cgcgctcctc gtgcagacag gtggtgcaac gagagcgttg
     9661 acacaggcgt cgccccgcgc gccggccagc acggttcgct gtccgccggc cgtcccgggc
     9721 tccaggcttc tcaagaggag aacgtcttca gtgaggacta ccgtcccctt caccgccctg
     9781 gcggtgatct cgatcgtcgt ggccgcgtgc agctcgtcgg cggcgacgcc gtcgcccacc
     9841 acggcccccc ctcccagccc ctcggcgagc gaggcgccgt ccgcgggcgc atccggcagc
     9901 gaggccggga gcccggccag ctcggcggcg gccgtcagca tcgacgactt cgattcgagc
     9961 ttctcggcga tggccacgct cgcacccgtc acgtccgccg gatcgggact ggtcggcgtg
    10021 atcctgcccg acgtcacgac gtccgcgcgc tataccagct acgacctccc gtacctcaag
    10081 caggccttcg aggccgccgg ctatccggcg accgacttca agatcgacaa cgcgtcgggc
    10141 accgccacga ccgagctcgc gctcgcccag gcggacatca cggccggcgc caaggtcctc
    10201 ctcgtcgatc cgctcgacag caccaccggc aaccagatcc agtcggccgc cgcggccgcc
    10261 ggcgtgccga tgatcagcta cgaccgcgcg acgttccagg gcaccgacac gtactacgtg
    10321 agcttcgaca accagcaggt cggcaagctg atcgggcaag gcctgatgga gtgcgtcagc
    10381 gactggggtg tgaccagccc ccaggtgttc accctcgacg gcggccagga caccgacccc
    10441 aacgccgtcg acttcgccaa gggctacaac tccgcgatct ggggccagcc cgtggcccag
    10501 gtcgccaagg gcgccaccaa cagcgctggc tacaccctcg tcaacgagca gatcgccccc
    10561 ggctgggaca acaaggccgg cggcacgatc ttccagcagg cctacaccgc ggacccgtcg
    10621 atcaacgcca cggtcgaggc caacgacggc ctcgccggcg ccgtcatcgg cgtcctcaag
    10681 gcggcgggcg tgccggccaa gaaggtcccg accaccggtc aggacgcgac cctccagggc
    10741 atggagttcg tgctccaggg ctatcagtgc gggtccgttt acaagccgat ctacctggag
    10801 gcccaggcag ccgctgcgct tgcgacgtac ctccgggccg gccagacgcc gccggcgggc
    10861 ctgatcaacg gcacgacgat cgacccggcc aactcggcga acacccagcc ggcggtgctg
    10921 ctcaccccgt cctgggtgaa tgccacgaac atggaatcga cggtgatcaa ggacaacttc
    10981 gtctccgcga cggacctgtg cgccgccgtc ggcgccagcg tgtgcacggc ggcgggcatc
    11041 cagtagctcg ccggctggcg acggcagcgg cgcgatcggc gccctgccgc cgccggcgcg
    11101 actcccccgc cagatgcagc gcgaggtcga cccatggaca tgaccaccgt agccccgccc
    11161 gccggggcaa ccgctgatgc aagtgcgccg ctgctggcgc tgcgcggcat cagcaagcac
    11221 ttcggcgcgg tccaggccct ctaccaggtc gatctcgacc tgccgtcgcg ccaggtgacg
    11281 gccctgtgcg gcgacaacgg cgccggcaag tccgtgctga ccaggagcat cgccggcatc
    11341 caccaggtgg atacgggcga gatctggtgg gaaggcaggc cggtccacat ccgcaacccc
    11401 agcgacgcgg cggcgctggg catcgagacc gtctaccagg atctcgcgct cgccgacaac
    11461 ctcgacatcg tccagaacat gttcctcggc cgcgagcggc tcgtccacgg gctcctcgac
    11521 gaggacgcga tggagcgcgc ggcggccgag accctcgcga gcctccgggt gaccacggtc
    11581 cgctcgatcc gccagcccgt ggcgacgctc tccggcggcc agcgccaggc ggtcgccgtc
    11641 gcgaaggccg tcatgtggaa ctcgaagctc gtgatgctcg acgagcccac ggccgcgctg
    11701 ggcgtcgccc agacgaggat ggtgctcgac ctgatcctgc agctcaagga acggggcctc
    11761 gcggtgatgc tcatctccca caacctcaac gacgtcttcg acgtggcgga ccggatcgcc
    11821 atcctgcggc tcggccggat ggtcgcccag gacgcgatcg gggccttcga ccgccagagc
    11881 gtggtcgact tcatgacgac gggcaggtcg cagcgcggcc tcacggcgaa ggcggtcggc
    11941 catggaccgc actgagtcgc tgccgcccgt gggtcccgcg ccgggcgtcg acgcggcgcc
    12001 ggacctgacg gccgcggtcg acctgccgac ccccgtgggc gcccagaccg tcggggaata
    12061 ccgccgcgcc tgggtcgcac gggtgcgggg cggcgacgcc ggcgtcctgc cggtcgccat
    12121 cgccctcgtg gcggtcgtga ccgtgttcac ggtcgtgagc cccaaccacg tgttcctgtc
    12181 cgcacgcaac ctcgtgaacc tgttcgacca gagcgcggtg ttcatcgtgc tggcgatggg
    12241 cgaggggttc gtgctgctgc tgggcgagat cgatctctcg atcggctacg tggcggccat
    12301 cggcggcatc gtcgccgcgc tgctcgtcca gcccagcccc ggctggccct ggcccgcggc
    12361 gatcgtcgcc gccctggtgg tcacgggcgc catcggcggg atccagggga cgatcatcac
    12421 ccgcctgggc atgccctcgt tcgtcgtcac gctcgcgggc tacctgatct ggttcggggt
    12481 gatgatcgtg atcctcggca cgtcggggca ggtcggcatc acgagcaccg tgcttcccgg
    12541 ccaggaggcg ctgtacggca tcgtctacag ctacctgtcg cccgtggccg gctgggtcgg
    12601 gctgatcgtc gtcgtcggcc tctacgggat cgtgacatgg cgccgggatg cccgccggcg
    12661 ccggagtggc ctcgtcgcgc cgccggtggg cctcaccgtt gcccggatcg cggcggccgc
    12721 agccgccggc gtcgtcgtcg tcgccatctg caacgtcgac cgcggcagct tcctctcggt
    12781 cgtgggtgtg ccgtgggtgg ttccgctcgt gctcgcggtg ctcgtgctgt ggtcgatgct
    12841 gctcgagcgc acgcagtacg gacgccacgt gtacgcggtc ggcggcaatg ccgaggcggc
    12901 ccgccgtgcg ggcgtcgggg ttccccgcgt ccggacagtc gcattcgtgc tgtgctccat
    12961 gaccgcaggc atcgccggga tcatctactg ctcgcagctg ggcggcatga cgacgaacat
    13021 caacggcggc cagctcgtgc tctacgcggt cgcggccgcg gtcatcggcg gcaccagcct
    13081 gtacggcggc cggggccgtg tcgtccacgg cctcctcggc gggctggtga tcggcgccat
    13141 ctacaacggc ctctacctgc tcggcctgcc gatccagtac cagctgatcg tcaccgggct
    13201 cgtcctcctg gccgccgtga gcgtcgattc gctgtcgcgg cgtggagcgg gcaccgggag
    13261 cgcgtacccg acctgacgcg tcgcgctcgc ccgacggcct ccggggcgcg ataagcgacg
    13321 gtgaagtggc gtccgctacg gttcgccgtg cccgtcgata catgccggcc ccgccgaccc
    13381 gtcgccccgc cgaggcccga accgccgggg ccggcacccc acccgcccgc cgaatgtcga
    13441 tacccgccgc aagagctgga ccggccgcag gaagccgcgc aggaaggcgc ggtgcggacc
    13501 ggtcggaagg agacacggac cgatgaacag ggtcctcatc accgggcgcc tcacgcgtga
    13561 tcccgagctg cgaacgacgg ccggcggcaa ggccgtcgcc cagttcgcag tcgccagcca
    13621 cgcgtacgtg tcaggcaagg agacgagcga gtttcacaac gtcgtcgcgt gggaccgcct
    13681 ggccgagacg tgcggccgct acctcggcaa gggccagctg gtcgcggtcg agggccggat
    13741 ccagacccgg tcctgggacg acgacaaggg agtccgccac tggaagacgg agatcgtcgc
    13801 gagcagcgtc gagatgctgt cggggcgccg caagaaggac tacgcggccg agacggccgc
    13861 caccgcgatg gatgcccagg cggccgcgga aggcttcgcg cccgacgcgg gcgagccggt
    13921 cgccgacgag gccgggttca gcgtggaccc gggcggcgac gacgtgtaag acgaagagga
    13981 cgagctcctc gaggtggccg tcgcggcggc ctgatcaacg actcctcctc ccggcggcgg
    14041 cgcggatccc ccctcccgcg ccgtcgccgc gcgtctacgg gacgtacagc gcacgtcgcg
    14101 gcaggcccgt cgcggctgcc acggtgcggg cagcgtcgcc gcggggcgac ccggcggcga
    14161 cgagctcgtc gacgcgcgcc cgcgccgctg cgagggcggc ctcggcgtcg tcgccatccg
    14221 cctccgcggc gctcccccac ccgaccacga tcacgatctc gccgcgagcc cggatctccc
    14281 catcggcggc gagggcagcg agctcgccca gcgacccgcg gacgatctgc tcgtggagct
    14341 tggtgagctc ccggcagacg gctccggggc gctccgcgcc gcaggacgcc gccagatccc
    14401 gaagcgtggc ggccatccgg cccggcgcct cgaactggcc ggttccacgc tcctccgcgg
    14461 cgatgcgcgc cagccgctgc cgacgctcgc gaccggaacg gggcaggaac ccctcgaacg
    14521 accagcgtgg ccccgctaca ccgctcgccg cgacggccgc cagcaccgac gaggcgcccg
    14581 gaatcggtac gacgctcccg ccggccgcgg cccacgcggc gacgagaccc tcgcccggat
    14641 ccgacacgac cggcgtgccc gcgtccgtga cgagcgcgag gttcgccccg ccctcgaggt
    14701 gcgccaggag ctcccgttcg cgtgcgggac cgctgcgcgc gtggtagctg accgcgcggg
    14761 tcgagatctc gtaccggtcg agcagtcggc ggctgatccg ggtgtcctcg gcggcgatca
    14821 acggaaccgc ccgcaggacc tccagtgccc gcagggtgac atcgccgagg ttcccgatcg
    14881 gcgtggccac gacgtacagc gtgccgccgc gcgccgcatc gccggtcgag gcggccgtca
    14941 ccgtcagggt cgagccgagc cgggccggcg ccgcgggtag aggtagtcga ccccggcggt
    15001 ccgcggcccg agcttcgcga tcggcggcac ctggcgcttg aactcggctc ccgcgaccat
    15061 ccggtcaacc cgctcgaccg tcgcccggtc gaagcccatc gcgaccatct cctcgacgga
    15121 gcgccgcttg tcgatccgcc agaacagcag gcggtcgagc tcgtggtagg tgaagccggc
    15181 ctcggtctcg tcggtctggc cgggccacag gtcggccgac ggcgccttgc ggatgatcgc
    15241 ctcgggcacg ccgatgtcgg ccgacacctg gcggacctgg ctcttgtaca ggtcgccgac
    15301 cgggttgaac gcgcacgccg agtccccgaa cagcgtcgtg tacccgatca gcgcctcggt
    15361 cttgttccca gtcccgacca cgagcccgcc ccacacgacc gatcggtcgt agagcacgct
    15421 catccgcatc cgcgccatca ggttcccccg ccgcagcggc gaggcagcga gtccttcgtc
    15481 gcccgccgcg ccggccacgc cgtcggcccc gtagtagccg tccaccatcg ggctgatgtc
    15541 gaccagctcc gacgcgcagc ccaggcgacg cacgacctcc tcggcgtcag cgcgggagtc
    15601 gggtgacgac gtccggtacg gcatcagcac acacagcagc ttgtccgccc cgatcgtctc
    15661 tgccaccagg tacgcgacga gcgccgaatc gatcccgccc gacagcccca ggaccgcgcg
    15721 gtcgaagccg gcctgggaca gctgcccccg gatgaactcc gcgacgaccc gccgcgcgac
    15781 gccggtgtcg atcgcgagct cgtccggcag gacgaacagc gcggcgccct cggtcatcgg
    15841 tcggacgacg tgcgggccgg ctcgaccgcg acgtcgaatc cgtcctggga tccgggctcc
    15901 gcgttcccgt ccgcagccag gccggaccgc tccgcgatca gccgccgcca ctcgcgcgcg
    15961 accagctccg ggcgctcgtc ccgcagcaac ggcagcgcga tccgctcccg gcgcacatcc
    16021 ccgaggtcga tgtcggcaaa gaacaggccc tcgtcgaaca ggggggccga gaagatcggg
    16081 gccccgccag gcccgatgac ctcggagccg ccccagaacg agatcgactc gtccaccccg
    16141 acgcggttgc agaacaccac gaacgacgtc gtcagctggg cgtaggtgcg catcagcgtc
    16201 cgccacgagt tcgccgtccc gaggcccacc tcgctggaac gggcgaggtc gcgacccggc
    16261 ga
//
LOCUS       DBNHXO010000471         2283 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3522026, whole genome shotgun sequence.
ACCESSION   DBNHXO010000471 DBNHXO010000000
VERSION     DBNHXO010000471.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3522026"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1646
                     /locus_tag="AC162A_09605"
     CDS             <1..1646
                     /locus_tag="AC162A_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gluzincin family metallopeptidase"
                     /protein_id="HIR5011140.1"
                     /translation="RSDSGVRVGPAFATFLAWAFGDKGSVRIEVPKPFDVDVTGQEMR
                     EQAAGDAKVFTATTADALGWYAWVNARNDDGLTREALELTGGEKIIVRGWPEDSRWRR
                     RVSAILTDSVPDLVGRIGLPWPVDGPLNVLEVHTPLLEGYAGFYDPKSDEITISENLD
                     DLTIVHEASHAWFNSGLFTDRWVNEGLAEEYAARVLAAETRGQVDPAKVKRSDKAAFP
                     LANWSPPAPIRDTQADAREQYGYDAAWTVIRAIVDETGEDGMRRVFRAAEDGTTAYAG
                     DGAPERAALPNDWRRFLDLTEELGGATTATDLLATWVLPSRDVAQLADRAASRAGYRT
                     LVGDEAGWAAPPAVRLALDKWAFNEADARIADSAAILHRRDGIDTRAASNDLDPPPDL
                     EAAYETAGTAADLTAAADLAAGTQRSLDDVVAASGAAGAPRDWLTTLGLSGKDPDADL
                     AAARAAWEAGNLTEATERAALVSGTLAVAAEAGRGRAMVIAVVFAIVIVLLAVLVLAW
                     IVRVRRRHRQVAMAAVAPAEPGWMAPPLEPPGPPAPPPG"
     gene            1707..2114
                     /locus_tag="AC162A_09610"
     CDS             1707..2114
                     /locus_tag="AC162A_09610"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5011141.1"
                     /translation="MDEDTGLPADDQESAYDLLQRGQGLLRRRHNAQAAIVLERAARL
                     EPGKASILEALGRAYFNSAQIEPARRTFEALIELDPSAPYAHFALGQSLKRLGCDREA
                     WTHLRLAVALAPASDLYRSALARMPDPRRDPAN"
BASE COUNT          325 a          840 c          826 g          292 t
ORIGIN      
        1 cgcgctcgga cagcggcgtg cgggtcgggc ccgcgttcgc gacgttcctc gcctgggcgt
       61 tcggcgacaa gggcagcgtg cggatcgagg tcccgaagcc cttcgacgtc gacgtcacgg
      121 gccaggagat gcgtgagcag gccgcgggcg acgccaaggt gttcaccgcg acgaccgcgg
      181 acgcgctcgg gtggtacgcg tgggtcaacg cgcgtaacga cgacgggctg acgcgcgaag
      241 ccctcgagct caccggcggc gagaagatca tcgttcgcgg ctggccggag gactcgcgct
      301 ggagacggcg ggtgtccgcg atcctcaccg acagcgtgcc cgacctcgtc gggcggatcg
      361 ggctcccatg gccggtcgac gggccgctca acgtgctcga ggtccacacg ccgctgctcg
      421 aggggtacgc gggcttctac gatcccaaga gcgacgagat cacgatcagc gagaacctcg
      481 acgacctcac gatcgtccac gaggcctcgc acgcgtggtt caacagcggc ctgttcaccg
      541 accgctgggt caacgagggg ctggccgagg agtacgcggc ccgcgtgctg gccgccgaga
      601 cgcggggcca ggtcgacccg gcgaaggtca agcggtccga caaggcggcc ttcccgctcg
      661 cgaactggtc gccgccggcg ccgatccgcg acacgcaggc cgacgcgcgg gagcagtacg
      721 gctacgacgc ggcgtggacg gtgatccggg cgatcgtcga cgagaccggc gaggacggga
      781 tgcggcgggt cttccgcgcg gccgaggacg ggacgacggc ctatgccggg gacggcgcac
      841 ccgagcgcgc cgcgctgccc aacgactggc gccgcttcct cgacctcacc gaagagctgg
      901 gcggcgcaac cacagccacc gacctgctcg cgacgtgggt cctgccctcg cgcgacgtgg
      961 cgcagctcgc cgatcgcgcg gcgtcccgcg ccgggtaccg cacgctcgtt ggcgacgagg
     1021 ccggttgggc cgcgccgccg gcggtccgcc tcgcgctcga caagtgggcg ttcaacgaag
     1081 ccgacgcccg gatcgccgac tcggccgcca tccttcaccg gcgcgacgga atcgacaccc
     1141 gtgccgcgtc caacgacctc gacccgccgc cggacctcga ggcggcctac gagaccgccg
     1201 ggacggccgc cgatctcacg gccgccgccg atctcgcggc cggtacccag cggtcgctcg
     1261 acgacgtggt cgccgcatcc ggcgcggccg gcgcaccgcg agactggctg acgacgctcg
     1321 gcctgagcgg caaggatccc gacgccgacc tcgccgcggc ccgcgccgcg tgggaggcag
     1381 ggaacctgac cgaggcgacc gagcgggcgg cgcttgtctc gggaacgctc gcggtcgcgg
     1441 ccgaggcggg tcgcggccgg gcgatggtga tcgccgtcgt gttcgcgatc gtgatcgtgc
     1501 tgctcgcggt cctcgtcctc gcctggatcg tccgggttcg ccgccgacac cggcaggtcg
     1561 cgatggccgc ggtcgccccg gccgaaccgg gttggatggc cccgccgctc gagccgcccg
     1621 gaccaccggc cccgcccccc ggctgacggg ctcggcgtca aagccccgtc acgacgcgat
     1681 ctcacggtcc gtggcaggat cgccagatgg acgaggacac gggcctgccg gcggacgatc
     1741 aggagtccgc gtacgacctg ctccagcgag gccagggcct gctccggcga cgccacaatg
     1801 cgcaggccgc gatcgtgctc gaacgggccg cgcggctcga gccgggcaag gcgtccatcc
     1861 tcgaggcgct tggtcgggcg tacttcaact ccgcccagat cgagcccgcg cggcggacct
     1921 tcgaggcctt gatcgagctc gatccgtcgg ctccgtacgc gcacttcgcg ctgggccaga
     1981 gcctcaagcg gctcggctgt gatcgcgagg cgtggacgca cctccgtctc gcggttgcgc
     2041 tggcaccggc ctccgatctc taccgctccg cgctcgcgcg gatgccggac ccgaggcgcg
     2101 acccggccaa ctaatcacgc cccggccccg tcatgcctgc agggcatggc tatcgggccg
     2161 aagggctcgc cgggccttcg tacgtcgctc gtccatgcct gcaggacaag gaaacgcgcg
     2221 gacgagacgc aattggcggc ctggggactc atttcgttgc cttgcaggca tacctcggcg
     2281 cca
//
LOCUS       DBNHXO010000472         1935 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1341100, whole genome shotgun sequence.
ACCESSION   DBNHXO010000472 DBNHXO010000000
VERSION     DBNHXO010000472.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1341100"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1822
                     /locus_tag="AC162A_09615"
     CDS             <1..1822
                     /locus_tag="AC162A_09615"
                     /inference="COORDINATES: protein motif:HMM:NF016736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HIR5011142.1"
                     /translation="RVGLVLPFGSRVATSRINFRLLAREAMSHGRRLDIVAPDASARA
                     LAASAGLPVFGSIGEYEAALDTEDDDKAARRAEARAAAGITGGAAAAGGAAAAGAAAV
                     AGAADGAPVAGGVRRTGKARGGSKAPEPDTIPPPDTIPPPDTIPPPDAAPLQPRDPIL
                     AEDSPIATGKPASIPVARPRRGRGRLGILVGVLLLVGIIGGAVAFGGYLLLPSTSITV
                     TPQIQAVGPVTFTVRADPSATSVDEAAAVVPAVTLTIPVSAQAEFPATGKRVQRTPAK
                     GAVRWTNCDPTSPYRIPSGTIVRTSGGVGFAIDEGVFLPVAILSGGGATPKLDCQTSE
                     VSVTAVEPGPAGNVDAGTIRVVPASYNRNVIRVNNPQATSGGTRKEFTRISPSDVEAA
                     LAQLDKDVRAQFATEVANPSDVPEGTTPFPETAVLGEPVPTTDPADLEGKEVDSFSLG
                     LTAEGTVLAADASPAEGIAGQRLEASVAEGYDLVPDSTSIEVGEGTVVDGVIEFPVTG
                     SARQVRPLDAAALERSVLGLPIEDARAVLAPYGDVVIVPWPDWVTTVPTLEQRVVLTI
                     AQPVDSGAVTESPPPASPASSPSAGLLDGGQAGQPVPSAG"
BASE COUNT          256 a          723 c          715 g          241 t
ORIGIN      
        1 gcgcgtcggc ctcgtgctgc cgttcgggtc gcgcgtggcg acgtcccgga tcaacttccg
       61 gctcctcgcc cgcgaggcca tgagccacgg ccgccggctc gacatcgtcg ctccggacgc
      121 ctcggcgcgc gccctcgcgg cctcggcggg cctgcccgtg ttcgggtcga tcggcgagta
      181 cgaggcggcg ctggacaccg aggacgacga taaggcggcg cgacgggcgg aggcacgggc
      241 ggccgccgga atcacgggtg gagcggcggc cgccggtggc gccgcggcgg ctggcgccgc
      301 agcggtcgcc ggagctgcgg atggcgcccc ggtcgccggc ggcgtacggc gcaccggcaa
      361 ggcacgtggc ggctcgaagg cgcctgagcc ggacacgatc ccgccgccag atacgatccc
      421 gccgccagat acgatcccgc cgccggacgc ggccccatta cagccccgcg acccgatcct
      481 cgccgaggac tcgcccatcg ccacgggcaa gccggcgtcc atcccggtcg cgcgcccgag
      541 gcgtgggcgc ggtcgcctcg gcatcctggt cggcgtcctg ttgctggtcg ggatcattgg
      601 cggggcggtg gcgttcggcg gctacctgct gctgccatcg acgtcgatca cggtcacccc
      661 gcagatccag gccgtcgggc cggtgacgtt cacggtccgg gccgacccct ccgccacatc
      721 ggtcgacgag gccgcggccg tggtcccggc cgtgacgctg acgatcccgg tctcggcgca
      781 ggcggaattc cccgccaccg gcaagcgcgt ccagcggacg ccggccaagg gcgcggtccg
      841 ctggaccaac tgcgacccga cgtcgcccta ccggatcccg tcggggacga tcgtccgcac
      901 ctcgggcggc gtgggcttcg cgatcgacga gggcgtgttc ctgcccgtgg caatcctgtc
      961 cggcggcggc gccacgccca agctcgactg ccagacgagc gaggtgtcgg tcacggcggt
     1021 cgagccgggt ccggcaggca acgtcgacgc cgggacgatc cgcgtcgtcc ccgcgagcta
     1081 caaccgcaac gtcatccgcg tgaacaaccc gcaggcgacg tcgggcggca cgcgcaagga
     1141 attcacccgg atctccccca gtgacgtgga agcggccctc gcgcagctcg acaaggacgt
     1201 ccgagcgcag ttcgccaccg aggtcgccaa cccgagcgac gtccccgagg gcaccacgcc
     1261 gttcccggaa accgcggtcc tgggggagcc cgtcccgacc accgatcccg cggacctcga
     1321 gggcaaggag gtcgactcgt tcagcctcgg gctcacggcc gagggcaccg tgctcgcggc
     1381 ggacgcgtcg cccgcggagg gcatcgccgg gcagcgcctg gaggcgtcgg tggccgaggg
     1441 ctatgacctc gtgccggact cgaccagcat cgaggtcggc gagggcacgg tggtggacgg
     1501 ggtgatcgag ttccccgtta ccgggtcggc caggcaggtc cgaccgctcg acgccgcggc
     1561 gttggagcga tcggtgctgg ggctgccgat cgaggacgcc agggccgtcc tcgcgccgta
     1621 cggcgacgtc gtgatcgtgc cctggcccga ctgggtgacg acggtcccga cgctggagca
     1681 gcgggtcgtc ctcacgatcg cgcagccggt cgacagcggg gccgtgaccg aatcgccgcc
     1741 ccccgcgtcg cccgcttcct cgcccagcgc gggcctcctc gacggcggtc aagcgggcca
     1801 gccggtacca tccgccggat gagccgcatc ctgggcgtcg atctcggcga acggcggatc
     1861 gggctcgcgg tggccgaatc ggcgatcggc ggtgcccggc cgctcgcgac gatcgcccgc
     1921 gccaagacca tcgag
//
LOCUS       DBNHXO010000473         1623 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3921494, whole genome shotgun sequence.
ACCESSION   DBNHXO010000473 DBNHXO010000000
VERSION     DBNHXO010000473.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3921494"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="AC162A_09620"
     CDS             complement(<1..192)
                     /locus_tag="AC162A_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011143.1"
                     /translation="MAIPSPSGGTTGGAVPDEIVQKAIADAATQAGVDPSAVTVVSAE
                     ATTWDSGALGCPKPGEMYTQ"
     gene            439..1395
                     /locus_tag="AC162A_09625"
     CDS             439..1395
                     /locus_tag="AC162A_09625"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HIR5011144.1"
                     /translation="MPLPSSIHAVIEFTFAPIAQVGPWSVRLETIALAVVVLVALILA
                     VRIARTTPVDVRRAAGDRLPPDGEPNHLRSDDLLFIAIAAIPGAVAGGRIGYVLLHLD
                     YYTANQGAILDVAQGGFELSLAVVGGTLTAAVVAALLGAPVGRWMHALILPLLLALAA
                     GKLAMVLGGDGQGIPWDGSLATAYLGPGPWGSLAPALPSHPSQAYEAFATVMVILLLS
                     ALLTLGRFERRAGGAFLLGIAMWAVARAIVATTWRDPAVIGPLNMGQVLALAVAAGML
                     VVLVVLVMVLRRRPLGPVEAPPEEAEDPSSPHWAEPTERPQI"
BASE COUNT          179 a          594 c          597 g          253 t
ORIGIN      
        1 ctgcgtgtac atctcgccgg gcttggggca gccgagggcg ccgctgtccc aggtcgtcgc
       61 ctcggcggac acgaccgtga ccgccgacgg gtccacgccg gcctgtgtcg cggcgtccgc
      121 gatcgccttc tggacgatct cgtccggcac cgcgccgccg gtcgtgccac cggatgggga
      181 ggggatggcc acctggctcc cgagtggccg ggtcgtcgga gcgtcgctcc cgccactggg
      241 gcccgcgggc gcccctggaa cggcggtgca gccgacgagg accgccaacg acaacagggc
      301 cagtgacgcg atgggcaggg agcggcggac ggggatcacc atgcccgctt ggacgcgcgg
      361 acccccgccc gtggttcccg cggccgcacc gcgcgcgcgg tcctcgggcg gccacgtggc
      421 cggtagactg cccgcgtcgt gccgctgccg tcgtccatcc atgccgtcat cgagttcacg
      481 tttgcgccga tcgcgcaggt cgggccgtgg agcgtgcgcc tggagacgat tgcgcttgcg
      541 gtggttgtcc tggtcgccct gatcctcgcg gtgcggatcg cccggacgac cccggtcgac
      601 gtccgccgcg ctgccggtga ccggctgccg ccggatggtg agccgaatca tcttcgttcc
      661 gacgacctcc tgttcattgc cattgccgcg atccccggcg cggtcgcggg ggggcggatc
      721 ggctacgtgc tgctgcatct cgactattac accgcgaatc agggcgcgat cctcgatgtc
      781 gcccagggcg ggttcgaact gtcgctcgcg gtcgtcggcg gcaccctcac cgcggcggtc
      841 gtcgccgcgc tgctcggcgc gcccgtcggc cgctggatgc acgccctgat cctgccgctg
      901 ctgctggccc tcgcggcggg caagctcgcg atggtgctgg gcggggacgg gcagggcatc
      961 ccgtgggacg gcagcctggc gacggcctac ctcggccccg gtccgtgggg ctcgctcgcg
     1021 ccggcgctcc cgtcacaccc ctcgcaggcg tacgaggcgt tcgcgacggt catggtgatc
     1081 ctgctgctgt cggcgctgct cacgctcggg cggttcgagc gtcgcgccgg cggcgcgttc
     1141 ctgttgggca tcgcgatgtg ggcggtggcc cgggccatcg tcgcgacgac gtggcgcgat
     1201 cccgcggtca tcgggccgct caacatgggc caggtgctcg cgctggccgt ggccgccggg
     1261 atgctcgtgg tgctggtggt cctcgtgatg gtgctcaggc gtcggcccct tggccctgtc
     1321 gaggctccgc cggaggaggc ggaggaccca tcgtcgccgc actgggcgga gccgaccgag
     1381 cggccgcaga tctagcggcg gggtgcaccc gtgcacggct gacctctcgc ccgctccgat
     1441 ccggagcgct agagtgacgg cctgaacatc cgaccgccat cgcgcctccg gaggccccca
     1501 tgacccaagt cgccgagtcc aaggacgcga caaccacatc gctgccgtcc atcggccatt
     1561 ggatcggcgg ccgccaggtc gccggccagt ccggccgtcg cggacccgtc ttcgacccgg
     1621 cga
//
LOCUS       DBNHXO010000474         3694 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3324928, whole genome shotgun sequence.
ACCESSION   DBNHXO010000474 DBNHXO010000000
VERSION     DBNHXO010000474.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3324928"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="AC162A_09630"
     CDS             <1..317
                     /locus_tag="AC162A_09630"
                     /inference="COORDINATES: protein motif:HMM:NF020037.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR5011145.1"
                     /translation="AWAILDGDRCFVEVSTSLAEIVEVPRATILGQRVEAFANPGDAS
                     VGSDFEGLWGELLANGELHATLRFARRDGTPREIEYHVTRDGAGPERHLAVVREIEPT
                     LP"
     gene            complement(325..774)
                     /locus_tag="AC162A_09635"
     CDS             complement(325..774)
                     /locus_tag="AC162A_09635"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5011146.1"
                     /translation="MRRVEAEALVGAARDEVWQLYDDIEGTPLWVPFVSEILYVSGPA
                     KVGTIYRERTRLGGIPSVAQWEIVEHRRPIRQVHVSDGFGMDSALIITFDGRGTGTWV
                     HQSAELRSRLWGPFGWLHEALLAIPAGWGVRAAVNGAKRAFEGDPPR"
     gene            complement(1063..2070)
                     /locus_tag="AC162A_09640"
     CDS             complement(1063..2070)
                     /locus_tag="AC162A_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5011147.1"
                     /translation="MRASRLVSLLLLLQTRGGMTAADLAEELEVSVRTIHRDVEALGA
                     AGVPIYAERGPHGGIRLVDGYRTRLTGMTAEEADALFLSGLPGPAAELGLGTVVAAAR
                     LKVLAALPTELRGRASRLLERFHLDAAGWFRAGETLPHLATIAQCVWDGDRIELDYER
                     SDTIVTRTLDPLGLVLKGGVWYLVASNGDQIRTYRVSRVRGIAPLPERAARPPGFDLA
                     TYWSESITAFERDTPRIEVTLRVRREAHRWLEDVIDGSALAAAVEEPDPEPDAWRRLR
                     ITLDWPREVAGRLLALGGAVEVVAPPELRTEIAALAAETVARYREPATAGVPIEAAPA
                     R"
     gene            2184..>3694
                     /locus_tag="AC162A_09645"
     CDS             2184..>3694
                     /locus_tag="AC162A_09645"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5011148.1"
                     /translation="MTPDETTEPTDQPAPADDEPVGFSALALDARLLRTLTALGYEEP
                     TPIQNAAIPPLLAGRDLLAEAPTGTGKTAAFALPILNRLAVRMDEDRASGRPVDGRGV
                     PRDGERASRPRALVLAPTRELAMQVAEAMHKYGRELGARVVAVYGGQPIAAQLHRLAR
                     GVDIIVATPGRTVDHLERKSVRLDEIETVVLDEADEMLDMGFADDLERILGALPEQRQ
                     TALFSATISGPIARLAGRHLRHPTEVRVHAEEAAGSTARVRQVAYVVRRADKLAALGR
                     VLDLEDGTATLVFARTRGEVDDLAEALSGRGRDAAALHGGLAQEQRDRIMARFRDGAL
                     DVLVATDVAARGLDIEQVTHVVNYDVPSSPDVYVHRIGRTGRAGREGVAITLVEPREH
                     RLLRDIERAIGVPLEIVGLPTIADVREKRLDTLRGTLRDALVSEGGNDRYRAVVEPLS
                     EEFDLVDIALAAIALADDEARGAEDTTELAPATLPPSFAGRDANKRGPRPAVAR"
BASE COUNT          497 a         1355 c         1359 g          483 t
ORIGIN      
        1 acgcctgggc gatcctcgac ggggatcggt gcttcgtcga ggtgtcgacg tcgctggccg
       61 agatcgtcga ggtgccgagg gcgacgatcc tcgggcagcg ggtcgaggcg ttcgcgaacc
      121 ccggcgatgc gtcggtgggc tccgacttcg agggcctgtg gggcgagctg ctggcgaacg
      181 gcgagctgca cgcgacgctt cgcttcgccc ggcgcgacgg cacgccgcgc gagatcgagt
      241 accacgtgac ccgggacgga gcgggtcccg agcgccacct tgcggtcgtc cgggagatcg
      301 agccgaccct gccctgatct gcgatcagcg gggtgggtcg ccctcgaagg cccgcttggc
      361 gccgttgacc gcggcgcgca cgccccagcc ggccgggatg gcgagcagcg cctcgtgcaa
      421 ccagccgaac ggcccccaca gccgcgagcg gagctcggcg gactggtgga cccaggtgcc
      481 cgttccgcgt ccgtcgaacg tgatgatcag cgcgctgtcc atcccgaacc cgtcggagac
      541 gtggacctgg cggatcgggc ggcggtgctc gacgatctcc cactgcgcga cgctggggat
      601 gccccccagg cgcgtgcgct cacggtagat cgtcccgacc ttggccggcc cggagacata
      661 gaggatctcg ctcacgaacg ggacccagag cggtgtgccc tcgatgtcgt cgtagagctg
      721 ccagacctcg tctcgcgcgg cgccaaccag cgcctcggcc tcgactcgtc gcatgtgggg
      781 ccagtatccc agagcctgcg agcccagcgt tgggcggggg cgtgctcagg gcaggtcggt
      841 gtcattcatg acggttgctg gcatgttccc ggcctacgat tcgggcatca agtgctcgat
      901 gtggacgagg tgatccatgc tcccgatgtc gctcatcgcc acggccatcc aggccgatcg
      961 cgagcgcgag ttccgcaacc gtcgcccccg cacggcgggt gtgccctccg ggctccggca
     1021 tccccggcgg ccggacgtcc aggccctcgg cgagggcccc cgtcagcggg cgggagccgc
     1081 ctcgatcgga acgccggcgg tcgctggctc ccggtagcgg gcgaccgtct cggccgccag
     1141 ggccgcgatc tccgtccgca gctccggcgg cgccacgacc tccacggcgc cgccgagggc
     1201 gaggagccgg ccggcgacct cgcgcggcca gtcaagggtg atgcgcagcc gtcgccacgc
     1261 gtcgggttcg ggatccggtt cctcgacggc cgccgcgagc gcgctcccgt cgatgacgtc
     1321 ctcgagccag cggtgggcct cccgtcggac acgcagcgtg acctcgatcc gcggcgtgtc
     1381 gcgttcgaac gccgtgatcg actccgacca gtacgtggcg aggtcgaagc cgggcggtcg
     1441 cgccgcccgc tcggggagtg gggcgatgcc gcggacccgc gagacgcgat aggtccggat
     1501 ctgatcgccg ttgctggcca cgaggtacca gacgccgccc ttgaggacga gccccaacgg
     1561 gtccagcgtc cgggtcacga tcgtgtccga acgctcgtag tcgagctcga tccgatcgcc
     1621 gtcccagacg cactgggcga tcgtggcgag gtgggggagc gtctcgccgg cccggaacca
     1681 gccggcggcg tcgaggtgga agcgctcgag cagccggctc gcgcggccgc gcagctcggt
     1741 cggcagcgcg gcgaggacct tgaggcgggc cgccgcgacg accgtcccca ggccgagctc
     1801 ggcggcgggc cccggcaggc ccgagaggaa cagcgcgtcg gcctcctcgg cggtcatgcc
     1861 tgtcagccgg gtgcggtagc cgtcgacgag ccggatgccg ccatggggtc cgcgctcggc
     1921 gtagatcggc acgccggcgg cgcccagcgc ctcgacgtcc cggtggatcg tgcggaccga
     1981 cacctcgagc tcctcggcga ggtccgcagc ggtcatgccg ccgcgcgtct ggagcagcag
     2041 gaggagcgag acgaggcggg acgcgcgcat ggcggcatgc tagcaaagcg gccattcagt
     2101 gtcaggttta ttcggtgccg gcactacctg cgggcagggc cgtcatcggc ggtctcacat
     2161 tcgatcgggt ctacgatctg tccgtgaccc ccgacgagac gaccgaaccc accgatcagc
     2221 ccgcgccggc cgacgacgag ccggtgggct tttccgccct cgccctcgat gcgcgcctgt
     2281 tgcggacact caccgccctg ggctacgaag aaccgacgcc gatccagaac gcggccatcc
     2341 cgccgctgct cgccggtcgc gacctgctgg ccgaggcgcc aaccgggacc ggcaagacgg
     2401 cggcgttcgc gctgccgatc ctcaaccggc tcgcggtgcg catggacgag gaccgcgcgt
     2461 cgggccggcc ggtggacggg cgaggcgtgc cgcgagacgg cgagcgggcc agccgcccgc
     2521 gagccctggt gctcgcgccg acgcgcgagc tcgcgatgca ggtcgccgag gcgatgcaca
     2581 agtacggtcg cgagctcggc gcgcgcgtgg tggccgtgta cggcgggcag ccgatcgcgg
     2641 cccagctcca ccggctcgcg cgcggggtcg acatcatcgt cgccacgcct ggccggaccg
     2701 tcgaccacct cgagcgcaag tccgtccggc tcgacgagat cgagacggtc gtgctcgacg
     2761 aggccgacga gatgctcgac atgggcttcg ccgacgacct cgagcggatc ctcggcgcgc
     2821 tgccggagca gcgccagacc gcgctgttct cggccacgat ctccgggccg atcgcccggc
     2881 tcgccgggcg ccacctgcgg catcccaccg aggtccgcgt ccatgccgag gaggccgcgg
     2941 gcagcacggc gcgcgtccgg caggtggcct acgtcgtccg ccgtgccgac aagctggcgg
     3001 cgcttgggcg cgtgctcgat ctcgaggacg gcaccgcgac cctcgtgttc gcgcggacgc
     3061 gcggcgaggt cgacgatctc gccgaggcgc tgagcggacg cggccgcgac gcggctgcgc
     3121 tccacggcgg cctcgcccag gagcagcgcg accggatcat ggcccgcttc cgcgacggcg
     3181 ccctcgacgt cctcgtcgcc accgacgtcg ccgcccgcgg cctcgacatc gagcaggtca
     3241 ctcacgtcgt caactacgac gtcccgtcgt cgcccgacgt ctacgtccac cgcattggcc
     3301 gcaccggccg ggcgggccgg gagggggtcg cgatcacgct ggtcgagccc cgggagcatc
     3361 gcctgctgcg ggacatcgag cgggcgatcg gcgtgccgct cgagatcgtg ggactgccga
     3421 ccatcgccga cgtccgcgag aagcgcctcg acaccctgcg cgggacgctc cgggatgcgc
     3481 tcgtgagcga gggcgggaac gaccgctatc gcgcggtcgt cgagccgctg tccgaggagt
     3541 tcgacctcgt cgacatcgcc ctcgcggcga tcgccctcgc cgacgacgag gcgcgcggcg
     3601 ccgaggacac gacggagctc gcgccggcca cgctcccgcc ctcgttcgcc gggcgcgacg
     3661 ccaacaagcg cgggccccgg ccggccgtgg cccg
//
LOCUS       DBNHXO010000475         3336 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_151374, whole genome shotgun sequence.
ACCESSION   DBNHXO010000475 DBNHXO010000000
VERSION     DBNHXO010000475.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_151374"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="AC162A_09650"
     CDS             <1..465
                     /locus_tag="AC162A_09650"
                     /inference="COORDINATES: protein motif:HMM:NF017984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase AlkZ-like family protein"
                     /protein_id="HIR5011149.1"
                     /translation="AATSDVAAWTRLTGLKEPMERLRPRLRTFRDERGRELFDVPGAP
                     IAEADVPAPVRFFPFYDNVYLSHADRARIIPEEARTSERSLVGGAAFSVDGFMAGFWT
                     TKGKGHERTITLEPMTPLSGAQRRETEAEALLLRRFLDAGGDAGPDPVRWST"
     gene            complement(491..1357)
                     /locus_tag="AC162A_09655"
     CDS             complement(491..1357)
                     /locus_tag="AC162A_09655"
                     /inference="COORDINATES: protein motif:HMM:NF016119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase domain-containing protein"
                     /protein_id="HIR5011150.1"
                     /translation="MLRRLTPLLAILLVVIVASPANALSLKREWRASVGGGANGSAVL
                     EAWTNGTGTLTASLKGLKRNTTYKVSLRKGSCNHPTKVLGRLGSVRTNASGAAPVSKT
                     IDSAQMAWVWWARSGKITARFTAGSSVKCAKFWYHRASRVVIPRYDIDLAVVKGPKGY
                     PYCNVAMYMRSLYQPTEPGVSFIYAHARTGMFLPLLNASKKHSGRAMIGKTVYVYTSD
                     SVRHTYRITKVRRHVRDISSSLKITDERLWLQTSEGPNSSYPKLIIVAERTGSSSVSY
                     DAAHPNAKPKRC"
     gene            complement(1545..1931)
                     /locus_tag="AC162A_09660"
     CDS             complement(1545..1931)
                     /locus_tag="AC162A_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011151.1"
                     /translation="MPRQFVVQVTNQPGQLALLAEQMAARGVDLRAIGGGGIGELGHM
                     IMTTADDDTARDVLETGGWTYFEGESVLAEVDDRPGGMARLARELADAGVNVHGHLFL
                     GRWSDRATFAFVVDDPEKARPILERR"
     gene            complement(1982..>3336)
                     /locus_tag="AC162A_09665"
     CDS             complement(1982..>3336)
                     /locus_tag="AC162A_09665"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033840 - alpha-1,4-glucan
                     glucosyltransferase (NDP-glucose donor) activity [Evidence
                     IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HIR5011152.1"
                     /translation="PVDVFLPRYRSVPVPDGALTEPTLTISDPDRARRGASMAVSIVN
                     VAADGYRLRLVDVPAAFDRDAFYDHPDDPWRFAVFCRAALAALRRDARPMDIVNVHDW
                     HTGPALLERARADAARDAFFAGTAVVATLHNLAYHGWTANDRIGELGLRVGEPLAGAN
                     PDGIDLLLTAIERSEIANTVSPGFAREALTPAFGMGLDGALRAKGNRFIGILNGIDPD
                     VWDPATDPALAAPYSREAPGGKATCRRDLLTRLGFDPDDDRVVLGMIGRMDPQKGFDL
                     LADGTPELLAAGARIVVQGSGHASLADPFRALAAAQPTSVALIERFDRDMARRIYAGV
                     DLFLMPSRFEPCGQGQMIALRYGTPPVVRRTGGLADSVIDATDRPQDGTGFVFADATP
                     GALVAAIARAAAMRTSDPAGWAALRDRGMAVDFRWDTGAAPRYLEAYRRAITLRRGDA
                     "
BASE COUNT          476 a         1151 c         1212 g          497 t
ORIGIN      
        1 gccgcgacga gcgatgtcgc cgcgtggacg cgactcacgg ggctcaagga gccgatggag
       61 cggctgcgac cgcgcctgcg aaccttccgc gacgagcgcg gccgcgagct gttcgatgtt
      121 cccggggccc cgatcgccga ggcggacgtt ccggcccctg tccggttctt ccccttctac
      181 gacaacgtct acctgtcaca cgccgatcgg gcccggatca tccccgagga ggcgcgcacg
      241 tccgagcgga gcctcgtcgg tggcgcggcg ttctcggtcg acggcttcat ggccgggttc
      301 tggaccacga agggcaaggg ccacgagcgc accatcacgc tcgagccgat gacgccgctc
      361 tccggggccc agcgtcgcga gacggaggcg gaggcgctgc tgctgcgtcg attcctcgac
      421 gctgggggcg acgccggacc ggatccggtg cgctggtcaa cctgagcgcg cgcctgcctc
      481 ggcgcgcagc tcagcagcgc ttgggcttgg cgttggggtg ggcggcgtcg tacgagaccg
      541 agctcgaacc ggtccgctcg gcgacgatga tcagcttggg ataggaactg ttcgggccct
      601 cggacgtctg gagccacagc cgctcgtcgg tgatcttgag cgatgagctg atgtcccgga
      661 cgtgccgcct gaccttcgtg atccggtagg tgtggcggac cgagtccgac gtgtagacgt
      721 agacggtctt gccgatcatc gcccggccgc tgtgcttctt cgacgcgttg agcagcggca
      781 ggaacatgcc cgtccgcgcg tgcgcataga tgaacgacac accgggctcg gtgggctggt
      841 acagcgatcg catgtacatc gccacgttgc agtacgggta gcccttgggc cccttcacca
      901 ccgccaggtc gatgtcatag cgcgggatga cgacccggct cgcacggtgg taccagaact
      961 tggcgcactt cacggacgat ccggccgtga aacgcgcggt gatctttccc gagcgcgccc
     1021 accacaccca ggccatctgg gcgctgtcga tggtcttgga caccggggcc gcgccggacg
     1081 cgttggtccg caccgaaccc agccttccga ggaccttcgt ggggtggtta cacgagccct
     1141 tgcggaggct gaccttgtag gtggtgttgc gcttgagtcc cttgaggctc gccgtcagcg
     1201 tgcccgttcc gtttgtccac gcctcgagga ctgcgctgcc gttcgcccct cccccgacgg
     1261 aggcccgcca ttcgcgcttc agcgagaggg cgttggccgg gctggcgacg atgacaacca
     1321 ggaggatggc gagcagcggc gtgaggcgac ggagcatggg cgagggtccc caagagcggt
     1381 tttgatggcg atgtggggtc gatgggccgc gtcgggccct gggcctgagc cctcaggatg
     1441 ccgtgcgagc gacgtcctga atatgtgttg cgtccagtct acggtgcatc cgcgcaccac
     1501 cggaatgacc tatccggggg tccccgaccg cgccaagagt cacgtcagcg acgctcgagg
     1561 atcggccgcg ccttctccgg atcgtcgacg acgaaggcga acgtcgcccg gtcgctccag
     1621 cgcccgagga acaggtgccc gtgcacgttg acgccggcgt cggcgagctc gcgggcaagc
     1681 ctggccatgc cgccaggccg atcgtcgacc tcggcgagca ccgactcgcc ctcgaagtag
     1741 gtccagccac cggtttcgag cacgtcgcgc gccgtgtcgt cgtccgccgt cgtcatgatc
     1801 atgtgcccga gctcgccgat gccgccgccg ccgatcgcgc gcaggtcgac gccgcgcgcg
     1861 gccatctgct cggcgagcag cgcgagctgg ccgggctggt tggtgacctg gacgacgaac
     1921 tgcctgggca ttggcggcct ccgggctggc gcccgttggg ggatcgggac ggagcccgat
     1981 tctacgcgtc gcccctgcgc agggtgatcg cgcgccggta cgcctcgaga tagcggggcg
     2041 ccgcccccgt gtcccagcgg aagtcgaccg ccatgccccg gtcgcggagc gccgcccagc
     2101 cggccgggtc cgaggtccgc atcgccgccg cccgcgcgat cgccgcgacc agcgccccgg
     2161 gcgtcgcgtc cgcgaacacg aagcccgttc cgtcctgcgg ccggtccgtt gcgtcgatga
     2221 cggaatcggc gagaccgccg gttcgtcgga cgaccggcgg cgtgccgtag cggagcgcga
     2281 tcatctggcc ctgaccacag ggctcgaacc gcgacggcat caggaacaga tcgacgccgg
     2341 cgtagatgcg acgggccatg tcgcggtcga agcgctcgat gagggcaacg ctggtcggct
     2401 gggcggccgc gagggcccgg aacggatcgg cgagggacgc gtgaccgctg ccctggacga
     2461 cgatccgcgc gcccgcggcc agcagctccg gggtcccgtc cgcgaggagg tcgaacccct
     2521 tctgggggtc catccggccg atcatgccga gcacgactcg gtcgtcgtcg ggatcgaacc
     2581 cgagcctggt cagcaggtcg cgccggcagg tcgccttccc tccgggcgcc tcacgggagt
     2641 aaggcgcggc aagcgccggg tcggtggccg ggtcccagac gtcgggatcg atgccgttga
     2701 ggatcccgat gaaccggttg cccttcgcgc gcagcgcgcc gtcgaggccc atcccgaacg
     2761 ccggcgtcag cgcctcgcgc gcgaacccgg gcgagacggt gttcgcgatc tcggatcgct
     2821 cgatcgccgt caacaggagg tcgatgccgt ccgggttcgc accggcgagt ggctcgccca
     2881 cgcgcaggcc gagctcgccg atccggtcgt tcgccgtcca gccgtggtag gcgaggttgt
     2941 ggagcgtcgc gacgacggcg gtcccggcga agaacgcgtc ccgggccgcg tccgcgcggg
     3001 ctcgctcgag cagcgccggg cctgtgtgcc agtcgtggac gttgacgatg tccatgggtc
     3061 gtgcgtcgcg tcgcaacgcg gccagcgccg cgcggcagaa cacggcgaac cgccacgggt
     3121 cgtcggggtg gtcgtagaac gcgtcacggt cgaacgccgc ggggacgtcc accaggcgca
     3181 gccggtagcc atcggccgcc acgttcacga tcgagacggc catagacgcc ccgcgacggg
     3241 cgcgatccgg atcagagatc gtcagggtgg gctcggtcag cgccccgtcc ggcaccggca
     3301 cggaccggta gcgcggcagg aagacgtcga ccgggg
//
LOCUS       DBNHXO010000476         1905 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1936787, whole genome shotgun sequence.
ACCESSION   DBNHXO010000476 DBNHXO010000000
VERSION     DBNHXO010000476.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1936787"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="AC162A_09670"
     CDS             <1..381
                     /locus_tag="AC162A_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_158066129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HIR5011153.1"
                     /translation="VQKTESAIRLTHLPTGIVVTCQNERSATQNRELAIRVLKSRLLE
                     LELEKREEELRKLRGEHVTAGWGNQIRSYVLHPYQMVKDLRSGYETSNTGAVLDGDLD
                     AFMQAELERVATGRAPSADDAPDE"
     gene            384..1382
                     /locus_tag="AC162A_09675"
     CDS             384..1382
                     /locus_tag="AC162A_09675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5011154.1"
                     /translation="MPPPVAVSYRQGLEGDLDACTRIWRIGIEDYQARLNQPPMPEDL
                     APLRRLLAHLLATDPERFWVACQGGTEPSIIGFSSASLREGLWFLAMLFVDPAAQADG
                     IGQALMDRAQASRDVDPGGPAIPSPDAPLDSGIHTWGMCTDAVQPISNGLYARRGMVP
                     RIPIWRLFGEVRRWSAVPSLPAGLVAVPFEQLELEGPDGERRLAETLDQVDRELLGAA
                     HPVDHAYLRREGRVGFLAREPGGRTLGYVYGSGVGRLGPVAALEPDLLPALIGTAIRE
                     TPAFGSVAAWVPGTAANALRAFLDAGLRIDGFPGMICWSRSDHPFERYLPISLAIV"
     gene            1544..>1905
                     /locus_tag="AC162A_09680"
     CDS             1544..>1905
                     /locus_tag="AC162A_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5011155.1"
                     /translation="MSPSPSPTFRERLTGRPRPAADDQPVVVLHDVTMRYPNGKEALK
                     DVELVVPEGDFVFLVGPSGAGKSTLTKLLIRDELATKGMVVVDGDDLARLNRRQVPKV
                     RRKIGIVFQDFKLLPRKSV"
BASE COUNT          266 a          696 c          660 g          283 t
ORIGIN      
        1 gtccagaaga ccgagtccgc gatccggctg acgcacctgc ccaccggcat cgtcgtgacc
       61 tgccagaacg agcggtccgc gacgcagaat cgagagctgg cgatccgggt gctcaagtcc
      121 cgcctgctcg agctggagct cgagaaacgc gaggaggagc tgcgcaagct gcgcggcgag
      181 cacgtcacgg ccggctgggg caaccagatc cgctcgtacg tcctgcaccc ctatcagatg
      241 gtcaaggacc tccgatccgg gtacgagacg tccaacacgg gggccgtcct cgacggcgac
      301 ctcgacgcgt tcatgcaggc cgagctcgaa cgagtcgcga ccggccgcgc accgtccgcc
      361 gacgacgcgc cggacgagta gacgtgccgc cgcccgtcgc cgtcagctac cggcaaggcc
      421 ttgaaggcga tctcgacgcg tgcacgcgga tctggcggat cgggatcgag gactatcagg
      481 cgcgcctcaa ccagccgccg atgcccgagg atctggcgcc cctgcgacgg ctgctggcgc
      541 acctgctggc gaccgacccg gagcggttct gggtcgcctg ccagggcggc accgagccgt
      601 ccatcatcgg cttcagcagc gcttcgctgc gcgaggggtt gtggttcctg gcgatgctgt
      661 tcgtcgaccc ggcggcgcag gccgacggca tcgggcaggc gctcatggac cgcgcgcagg
      721 cgagccggga cgtggaccct ggtggtccgg cgatcccctc gcccgacgcg ccgctcgact
      781 ccggcatcca cacctggggc atgtgcaccg atgccgtcca gccgatctcg aacgggctct
      841 acgcgcgacg cggcatggtg ccccgcattc cgatctggcg cctgttcggc gaggtgcggc
      901 gctggtcggc ggtgccgtcg ctccccgccg ggctcgtggc ggtgccgttc gagcagctcg
      961 agctcgaggg ccccgatggc gagcgacgcc tggccgagac cctcgaccag gtcgaccgcg
     1021 agctgctggg agcggcccat ccggtcgatc atgcgtacct gcgccgcgag gggcgcgttg
     1081 gatttctcgc ccgcgagccg ggcggccgga cgctcggcta cgtctacggt tcgggtgtcg
     1141 ggcggctggg tccggtcgcc gcgctggagc cggacctcct gccggcgctg atcggcacgg
     1201 cgatccgcga gaccccggcg ttcggctcgg tcgccgcctg ggtgccgggc accgccgcca
     1261 acgcgttgcg cgcgttcctc gacgccgggc tccgcatcga cggcttcccc gggatgatct
     1321 gctggtcgag gtccgaccac cccttcgaac gctacctgcc gatctcgctc gcgatcgtct
     1381 gaggtttgcc ggtcccggca ccggatgtta gcctcgggta gacgtggcct caccgaccgt
     1441 gcccgtgggc ggagacgtcg ccccgcccgg catcccgagc ggcaacccca ccggtagtcc
     1501 ggtccccgcc tcgaccccgc cgtcaccgag cacacccttg accgtgtccc cgagcccgtc
     1561 gccgaccttc cgcgagcgcc tgaccggtcg accgcgcccg gcggccgacg accagccggt
     1621 cgtcgtgctg cacgatgtga cgatgcgcta tcccaacggc aaggaggccc tcaaggacgt
     1681 cgagctcgtc gtccctgagg gcgacttcgt gttcctcgtc ggaccgtcgg gggcgggcaa
     1741 gtccacgctc acgaagctgc tgatccgcga tgagctggcg accaagggca tggtcgtcgt
     1801 ggacggggac gacctcgccc ggctcaaccg gcgccaggtc cccaaggtgc gacggaagat
     1861 cggcatcgtg ttccaggact tcaagttgct gccccgcaag tccgt
//
LOCUS       DBNHXO010000477         2819 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1344048, whole genome shotgun sequence.
ACCESSION   DBNHXO010000477 DBNHXO010000000
VERSION     DBNHXO010000477.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1344048"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..972)
                     /locus_tag="AC162A_09685"
     CDS             complement(46..972)
                     /locus_tag="AC162A_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5011156.1"
                     /translation="MRIVSLIPSATEIVFALGLGDELVGVSEDCDFPPDVRHIPVVSR
                     RTHDLSTATSGEIHATIEASMHDHSPLYVLDDAALAAAAPDLIITQELCRVCAVGYRE
                     VNEVARRLDGDVTVISLEPTSIEGILNSIQTVGAMTEAEDAAVDVVEGLRERLQAVEE
                     IVVGRRDHGFAPPRVAAIEWLDPPFAVGHWVPEQVRLAGGWELLGAEGGRSVETTWAA
                     LGEVDPEILVLMPCGFGLAETMAEWRRTVKPPVWPELRAVRDGRVFAVDGSGLFSRPG
                     PRVVDGIEVLAELIDPAAFDGMSPPETWARVD"
     gene            complement(1013..1300)
                     /locus_tag="AC162A_09690"
     CDS             complement(1013..1300)
                     /locus_tag="AC162A_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5011157.1"
                     /translation="MAYVIAEPCIDVLDESCVSVCPVDCIHFDAGTDRMLAIDPNECI
                     DCGACEPECPVNAIFPEESLPPEWAKFTQINAVWFTDKAEARAAVDGIKPA"
     gene            complement(1415..1963)
                     /locus_tag="AC162A_09695"
     CDS             complement(1415..1963)
                     /locus_tag="AC162A_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011158.1"
                     /translation="MQRPGILLASALLLSACDVGAPAATPPITPGTSGSPREVNVVTR
                     DYAFVPSIVDLAPGETVLLHVINGGLERHEAVFGSFGDQLAWETAEAATVGAPPGPTP
                     FVPTPPGFDGVRVVVGSGERVDVTWTVPADATVAPGGWFVGCHIPGHWQKGMVAPIRL
                     VGPDGRPLGTPPAMPTIAPPAT"
     gene            2237..>2819
                     /locus_tag="AC162A_09700"
     CDS             2237..>2819
                     /locus_tag="AC162A_09700"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017556112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5011159.1"
                     /translation="MPAVRLEGVEKRYGDVVAVAGIDLEIRDGEFFSMLGPSGSGKTT
                     TLRMIAGFELPTAGRVLLHGRDVTRLAPFERDVNTVFQDYALFPHMTVGDNVAYGLLV
                     RKVPKAERQRRVGDALRMVRLEGYDRRKPGQLSGGQRQRVALARALVNRPRVLLLDEP
                     LGALDLKLREEMQIELHQIQQDVGITFIYVTHDQ"
BASE COUNT          415 a         1018 c         1011 g          375 t
ORIGIN      
        1 agtcgaaggt gttgcggaac ggcgacgacg gggtcatcgg cgcgctcagt cgacgcgcgc
       61 ccacgtctcg ggcggcgaca tcccgtcgaa ggcggcggga tcgatgagct cggccaggac
      121 ctcgatcccg tccacgaccc tcgggcccgg ccgcgagaac aggcccgacc cgtcgacagc
      181 gaacacccga ccgtcgcgga ccgcgcgcag ctcgggccag accggtggct tcacggtccg
      241 ccgccactcg gccatggtct cggccagccc gaacccgcag ggcatcagca cgaggatctc
      301 tgggtcgacc tcgccgagcg cggcccaggt ggtctcgacg gaccggccac cctcggcgcc
      361 aaggagctcc cagccgcccg cgagccgcac ctgctcgggc acccagtggc cgacggcgaa
      421 cggcggatcc agccactcga tcgcggccac acggggcggc gcgaacccgt gatcccgccg
      481 cccgaccacg atctcctcga ccgcctggag ccgctcgcgc agcccctcga ccacatcgac
      541 ggcggcgtcc tcggcctcgg tcatcgcgcc caccgtctgg atcgagttga ggatcccctc
      601 gatcgaggtc ggctcgaggc tgatcaccgt cacgtccccg tcgaggcgcc gggcgacctc
      661 gttgacctcg cgatagccga ccgcgcagac gcggcacagc tcctgggtga tgatcaggtc
      721 cggcgcggcc gccgcgagcg cggcgtcgtc cagcacgtac agcggcgagt ggtcgtgcat
      781 cgaggcctcg atcgtcgcgt ggatctcgcc cgaggtcgcc gtcgagaggt cgtgggtccg
      841 ccggctgacg accgggatgt ggcggacgtc gggcggaaag tcgcagtcct cgctgacgcc
      901 gaccagctcg tcacccaggc ccagcgcgaa cacgatctcg gtcgcggacg ggatcaggga
      961 gacgatgcgc accgatcaag tgtaggggcg ggggcgtcgc ggccccgcga ggtcaggcgg
     1021 gcttgatccc gtcgacggcg gcgcgcgcct cggccttgtc ggtgaaccac acggcgttga
     1081 tctgggtgaa cttcgcccac tcgggaggca ggctctcctc cgggaagatc gcgttcaccg
     1141 ggcactcggg ctcgcacgcg ccacagtcga tgcactcgtt gggatcgatc gcgagcatgc
     1201 gatcggtgcc ggcgtcgaag tggatgcagt cgaccgggca gacggacacg cacgactcgt
     1261 cgaggacgtc gatgcagggc tcggcaatca cgtacgccat gatccgcagg ctctcccgat
     1321 gtgtgcgtgg cagcgggcct cgcgcggtct ggctcgcgcg ccgcaagaat gtaccaccgg
     1381 aggcctgagc ttcggtgctg tccggggcac ggtgtcaggt cgccggagga gcgattgtcg
     1441 gcatggcggg cggcgtcccc agtggccggc cgtcggggcc caccagtcgg atcggggcga
     1501 ccatcccctt ctgccagtga ccggggatgt ggcagccgac gaaccacccg cctggcgcga
     1561 ccgtcgcgtc cgcggggacg gtccaggtca cgtcgacccg ttcgccggag ccgacgacga
     1621 cccggacacc gtcgaagccg ggcggcgtcg ggacgaacgg cgtcggaccc ggcggggcac
     1681 cgacggtcgc cgcctcggcc gtctcccagg ccagctgatc gccgaagctc ccgaacaccg
     1741 cctcgtgccg ctcgaggcca ccgttgatca cgtggaggag cacggtctcc ccgggcgcga
     1801 ggtcgacgat cgatgggacg aacgcgtagt cgcgcgtcac gacgttcacc tcgcgcgggc
     1861 tgccgctcgt gcccggcgtg atcggtggcg tggcggcggg cgcgccgacg tcgcacgcgc
     1921 tcaagagaag cgcggacgcg aggaggatgc cgggtcgctg cacccggcca tcatgctgga
     1981 caccgcaggg cggaaagcgc ttgacatcgc ggcgcaccga cctctagcgt gcgcgccact
     2041 tcgggcggcc tgcgcatggg gtacggggag cgcgccgacg ccccagcccg gccgcgttcc
     2101 gcggcgtttt ggagacttgc gaccccgcgt gacgagcaac cctgccgcca ccacgaccac
     2161 cgagccagcc gacggcccgg ccgctgcggg gacgcgcgcc tccggtgccg ccgagtcgtc
     2221 cggcccgggc gccacggtgc cggcggtcag gctcgagggc gtcgagaagc gctatggcga
     2281 cgttgtggcc gtggccggca tcgacctcga gatccgcgac ggcgagttct tctcgatgct
     2341 cgggccgtcc gggtcgggca agacgaccac gctgcggatg atcgcggggt tcgagctccc
     2401 gacggcgggc cgcgtgctgc tccacggccg ggatgtaacg cgcctcgcgc cgttcgagcg
     2461 tgacgtcaac accgtgttcc aggactacgc cctgttcccg cacatgaccg tcggcgacaa
     2521 cgtcgcctac ggcctgctcg tgcgcaaggt ccccaaggcg gagcgccagc ggcgcgtcgg
     2581 cgatgcgctg cggatggtcc gtctcgaagg ctacgaccgg cgcaagcccg gccagctctc
     2641 gggtggccag cgccagcgcg tggcgctcgc ccgagcgctc gtcaaccggc cgcgcgtgct
     2701 gctcctcgac gagccgctgg gcgccctcga tctcaagctg cgcgaggaga tgcagatcga
     2761 gctgcaccag atccagcagg acgtcgggat cacgttcatc tacgtgaccc acgaccagg
//
LOCUS       DBNHXO010000478         2827 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3128873, whole genome shotgun sequence.
ACCESSION   DBNHXO010000478 DBNHXO010000000
VERSION     DBNHXO010000478.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3128873"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /gene="argC"
                     /locus_tag="AC162A_09705"
     CDS             <1..976
                     /gene="argC"
                     /locus_tag="AC162A_09705"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR5011160.1"
                     /translation="PNVAIVGLVGRERRGDPLAGIHPHLATEGLRVFDAVPDEAEGVF
                     LALPHGAVAARIDEFLGAGLTVIDLGPDFRLRDPEDYPRWYHFDHPRPDLLATAVYGL
                     PELHRAQLAAAGGKPNAIVGAPGCYATTSILALAPLARAGLIADLVVDAKSGVSGAGR
                     EPKADLQFGEVNESVKAYGIFTHRHIGEIEQELGALGGGLAANPGVSGVDFLPHLIPM
                     TRGILSAGHVRTTRSVTQAELDDLYHDAYADEPFVTVTEDAPATKHVYGSNEARVWCR
                     LDERNGRVLAIGVLDNLVKGAAGQAIQAFNLVHGLPETAGLLQLPLAP"
     gene            973..2352
                     /gene="argJ"
                     /locus_tag="AC162A_09710"
     CDS             973..2352
                     /gene="argJ"
                     /locus_tag="AC162A_09710"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-CoA as donor [Evidence IEA];
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HIR5011161.1"
                     /translation="MTLATDPVLAPVVAALPPVERAARIPGGFSAAGATVGIKASGRP
                     DLVLIVADDGPVPAAAVFTPNRFAAAPVLLSRSNLQATGGGSAAAGLARAIVSTSGCA
                     NAATEAAGDRDQAAIGAALASALGAAEAEILHLSTGIIGTRLPVDIVRAGIERVLGGG
                     LGATDAALASAAAALRTTDTTTKMATTTVSVPAASGGGTVEVTVTGIAKGVGMIHPNM
                     ATMLSVVLTDATADPATLHGLLRGTAARTWDQLSVDGDTSTNDTVFLLASGKAGAADA
                     ATDQGARAALGAAIEAVARDLARQQASDGEGATALVTCQVTGATDDADARAIARAVIS
                     SSLVKAAVHGRDPNWGRIAGAAGNATLAQRAVLEASGLDSSEAAERAGAPAALNTSTL
                     RISIAGHLVYDGPSGGPVEVDKAAAREAMGGAEVLIRLDLGLGGGTGEAFGCDLTEEY
                     VRENSEYTT"
     gene            2349..>2827
                     /locus_tag="AC162A_09715"
     CDS             2349..>2827
                     /locus_tag="AC162A_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR5011162.1"
                     /translation="MSEIVVVKLGGTTLADQRQVLAEVAAVARKRPVVLVHGGGKRMT
                     EWLERLGVPTHFEGGLRVTDPAALEVAAAVLRGVVNSELVAALRDVGCDAVGLSGVDG
                     GLLIGQRLPNVGLVATVVGIRRDLIDSLLVADQVPVVAPLARDEDGVVCNVNADDAAA
                     "
BASE COUNT          371 a         1019 c         1061 g          376 t
ORIGIN      
        1 cccgaacgtc gccatcgtcg gcctcgtggg gcgcgagcgg cggggcgatc ccctggccgg
       61 catccacccc caccttgcga ctgaggggct gcgcgtgttc gacgccgtcc ccgacgaggc
      121 cgagggcgtg ttcctcgccc tgcctcacgg cgcggtcgcg gcgcggatcg acgagttcct
      181 cggggccggg ctgaccgtca tcgacctcgg ccccgacttc cgcctgcgcg atcccgagga
      241 ctacccgcgc tggtaccact tcgaccatcc ccggcccgac ctcctggcga ccgcggtcta
      301 cggcctgccg gagctccatc gggcccagct ggccgcggcg ggcggcaagc ccaacgccat
      361 cgtcggcgcg ccggggtgct acgccacgac ctcgatcctc gcactggccc cgctggcgcg
      421 tgcgggcctg atcgcggacc tcgtcgttga cgccaagagc ggcgtgtccg gcgccggccg
      481 cgaacccaag gcggacctcc agttcggcga ggtcaacgag agcgtcaagg cgtacggcat
      541 tttcacccac cgccacatcg gcgagatcga acaggagctc ggcgcgctgg gcggcgggct
      601 ggccgccaac ccgggggtca gcggcgtcga cttcctgccc cacctgatcc cgatgacgcg
      661 gggcattctg tcagcgggac acgtgcggac gacgcgatct gtcacgcagg cggagctcga
      721 cgacctgtac cacgacgcct acgcggacga gccgttcgtg acggtgaccg aggacgcgcc
      781 ggcgacgaag cacgtctacg gcagcaacga ggcccgcgtt tggtgccggc tcgacgagcg
      841 caacggccgg gtgctggcga tcggcgtgct cgacaacctg gtcaagggcg cggccgggca
      901 ggcgatccag gcgttcaact tggtccacgg cctgcccgag acggccggcc tgctgcagct
      961 gccgctcgcg ccgtgacgct cgcgaccgat cccgtgctgg cgccggtcgt ggcggccctg
     1021 ccgcccgtcg agcgcgcggc tcggatcccc ggggggttct ccgcggccgg ggcgacggtc
     1081 gggatcaagg cctcgggccg gccggatctc gtgctgatcg tggccgacga cggtccggtc
     1141 ccggcggcgg cggtcttcac gccgaaccgg ttcgccgcgg cgcccgtgct gctctcgcga
     1201 tcgaaccttc aggcgactgg tggcggctcg gccgcggctg gtctcgcacg cgcgatcgtc
     1261 tccacgtcgg gctgcgcgaa tgccgcgacg gaagcggcgg gagaccggga tcaggcggcc
     1321 atcggcgcgg cgctcgcgtc ggcgctcggc gccgccgagg cggaaatcct ccacctgtcc
     1381 accgggatca tcgggacccg gcttccggtc gacatcgtca gggcgggcat cgagcgggtc
     1441 ctcggtggcg ggctcggagc gacggacgcg gcgctcgcat cggcggccgc ggcgctgcgc
     1501 acgaccgata cgacgaccaa gatggcgacg acgacggtgt cggtgccggc agcttcaggt
     1561 ggcggcacgg tggaggtcac cgtgaccggg atcgccaagg gtgtcgggat gatccatccc
     1621 aacatggcga cgatgctgtc ggtggtcctg acggacgcga ccgcggaccc cgcaacgctg
     1681 catgggctgc tgcgcgggac ggccgcccgc acgtgggacc agctctcggt cgacggcgac
     1741 accagcacca acgacacggt gttcctcctg gcttcgggca aggcgggggc ggccgacgcc
     1801 gcgacggacc agggtgcccg ggccgcgctg ggtgccgcga tcgaggcggt cgcccgcgac
     1861 ctcgctcgtc agcaggcctc cgacggcgag ggcgcgacgg cgctcgtcac ctgccaggtc
     1921 accggtgcca ctgacgacgc ggacgcgcgc gccattgccc gcgccgtgat ctcgtcgtcg
     1981 ctggtcaagg ccgcggtcca cgggcgcgac cccaactggg gccggatcgc cggtgcggcc
     2041 ggcaatgcga cgctcgccca gcgggccgtc ctcgaggcgt ccgggcttga ctcgagcgag
     2101 gcggccgaga gggccggcgc acccgcggca ctcaacacga gcacgctccg gatctccatc
     2161 gccggccacc tcgtgtacga cggcccgtcc ggcggcccgg tcgaggtcga caaggccgcg
     2221 gcccgcgagg cgatgggcgg cgccgaggtc ctgatccgac tcgacctcgg gctcggcggg
     2281 ggcaccggcg aggccttcgg ctgcgacctc acggaagagt acgtacgaga gaacagcgag
     2341 tacacgacgt gagcgagatc gtcgttgtca agctcggtgg gacgaccctt gcggatcagc
     2401 ggcaggtgct cgcggaggtc gccgcggtcg cgcgcaagcg gccggtcgtg cttgtgcacg
     2461 gcggcggcaa gcggatgacc gaatggctcg agcgccttgg cgtcccgacg cacttcgagg
     2521 gtggcctgcg ggtcaccgat ccggcggcgc tcgaggtcgc ggcggcggtg ctgcgcggcg
     2581 tcgtgaacag cgagctcgtg gccgcgctgc gggacgtcgg ctgcgacgcg gtcgggctct
     2641 cgggcgtgga tggcgggctg ctcatcggcc agcgcctgcc caacgtcggg ctggtcgcga
     2701 ccgtcgtcgg gatccggcgc gacctcatcg actcgctgct cgtcgccgac caggtcccgg
     2761 tcgtcgcacc gctcgcgcgg gacgaggacg gcgtcgtgtg caacgtcaac gccgacgacg
     2821 cggccgc
//
LOCUS       DBNHXO010000479         2757 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2731891, whole genome shotgun sequence.
ACCESSION   DBNHXO010000479 DBNHXO010000000
VERSION     DBNHXO010000479.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2731891"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="AC162A_09720"
     CDS             complement(<1..375)
                     /locus_tag="AC162A_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011163.1"
                     /translation="MHTHLDETLDPEAPGAAVQPPDSESAEQAVAAEEAVAAEAPEAP
                     GPADTPEPPDIAEPVGVMDLAATAPVESVDAVEGASDAPAADEGTDSDEPTEPDETTE
                     PDETTEPEAVAAPALPSNVAELI"
     gene            complement(563..1054)
                     /locus_tag="AC162A_09725"
     CDS             complement(563..1054)
                     /locus_tag="AC162A_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HIR5011164.1"
                     /translation="MAGEVSFDVVSDFDEQELRNALDQVRREVQQRYDFKGATVDLTQ
                     EKTQIVLVTNDDFRARAVRDLIESKAVRRNLSLKIFDWGVVEPSGGNKVREVITLRRG
                     LTDDLARKLVKQIRDEFPKVQPRIQGDAIRVSAKSKDELQRVISRLRELDLEVPLQFE
                     NYR"
     gene            complement(1066..1452)
                     /locus_tag="AC162A_09730"
     CDS             complement(1066..1452)
                     /locus_tag="AC162A_09730"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5011165.1"
                     /translation="MTLTYPAGFDLLEAFHKARTGYDGDAFVDLFTDEAELVLDPFEP
                     PLAGHNALRAYLLEAADAERHYDLAIERHWVAGDTLLAAWHASWNRSDDDAKVRQAGF
                     LSAEVGGDGRIVRLRLWTVTREHLAG"
     gene            complement(1449..>2757)
                     /locus_tag="AC162A_09735"
     CDS             complement(1449..>2757)
                     /locus_tag="AC162A_09735"
                     /inference="COORDINATES: protein motif:HMM:NF019850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR5011166.1"
                     /translation="RLAVTKALTAIDPGNAELAAARAAGIPLEPWQQVVADAAAGRRL
                     VGIAGTHGKSTSAGWLVHVLAAAGRDPGAFVGALLPAALTGLGVPATARRGGGDVFVV
                     EADEYAGNFDAYRPSVIVLTSAEWDHPDVFADRGAVLAAFEAWIRRAAPTGGDSGTCL
                     VANVGDEGVAELVDRLADWPGRILATALVDVAPQRLGGFARGIAERFRSASGPADTLL
                     GRITSAEPDATVLEIAGLDPLAGPLPVRLPTAGRHNAANALGVAGAAAAVGVPAPAVI
                     AALAGFGGVGRRLERKGEAHGVVVYDDYGHHPTAIRETLAAVRQREPGRRVWAVYEPL
                     TYHRTAALLDAFADALAGADAVAIADIWAGRDPDTSISSPADLAAAVARRAPAIPVAA
                     PGSVEATAAWLAGEVRARDAVLVMGGGRSYRIGELLLEHLEAR"
BASE COUNT          364 a         1009 c          998 g          386 t
ORIGIN      
        1 gatcagctcg gcgacgttcg aggggagggc gggggcggcg accgcctccg gctcggtcgt
       61 ctcgtccggc tcggtcgtct cgtccggctc ggtcggctcg tccgaatcgg tcccctcgtc
      121 ggccgccggc gcgtcgcttg cgccctccac cgcgtcgaca gattcgaccg gagccgtcgc
      181 ggcgaggtcc atgacgccga cgggctcggc gatgtcgggg ggctccgggg tgtcggcggg
      241 cccgggtgcc tcgggtgcct cggcagccac ggcctcctcg gcagccacgg cctgctcggc
      301 ggactccgag tcgggcggct ggaccgcggc gccgggcgcc tcggggtcga gcgtctcgtc
      361 gaggtgggtg tgcatcgcga ccctcctgtg ccggcctggg cggcggtccg tccgtggccg
      421 gggcgtgcga acgttgacgg gtcgctaggg acccactggg gtggccaacg ggatcatcgc
      481 cgcgagcatg cccgcgacga cgaccacggc cacgatgatc atgaggatcc tggcggttct
      541 tcccgatccg ggcatgccgc gcctaccggt agttctcgaa ctgcagcggg acctcgaggt
      601 ccagctcgcg cagtcggctg atcacccgct ggagctcgtc cttgctcttc gcggagacgc
      661 ggatcgcatc accctggatc cggggctgga ccttgggaaa ctcgtcccgg atctgcttga
      721 cgagcttgcg ggccaggtcg tcggtcaggc cgcggcgaag ggtgatgacc tcgcggacct
      781 tgttgccacc cgagggctcg acgacgcccc aatcgaagat cttgagcgac aggttgcgac
      841 gaaccgcctt ggactcgatg aggtcgcgca cggcacgagc ccggaagtcg tcgttcgtga
      901 ccagcacgat ctgcgtcttc tcctgggtca ggtcgaccgt ggcgcccttg aagtcgtagc
      961 gctgctggac ctcgcgccgc acctggtcga gcgcgttgcg cagctcctgc tcgtcgaagt
     1021 ccgagacgac gtcgaacgag acctcccctg ccattcgcgc tcctctcagc ccgcgaggtg
     1081 ctcgcgggtc actgtccaca gccgcagacg cacgatgcgc ccgtcccctc ccacctcggc
     1141 ggagaggaag ccggcctgcc ggaccttcgc gtcatcgtcg ctgcggttcc agctggcatg
     1201 ccaggcggcc aggagcgtgt cccccgcgac ccagtggcgc tcgatggcca ggtcgtagtg
     1261 gcgttccgcg tcggccgctt ccaacaagta tgcccgcagc gcgttgtgac cggcgagcgg
     1321 gggctcgaac gggtcgagga cgagctcggc ctcgtcggtg aacaggtcga caaaagcatc
     1381 accgtcgtac ccggtgcgcg ccttgtggaa cgcctcgagc aggtcgaagc cggcaggata
     1441 cgtcagcgtc atcgtgcctc caggtgctcg agcaggagct cgccgatgcg gtaggaacgg
     1501 cccccgccca tgacgaggac cgcatcgcgg gcacggacct cgccggccag ccaggcggcg
     1561 gttgcctcga cgctccccgg tgccgcgacc gggatcgctg gcgcgcgccg tgcgacggcg
     1621 gcggcgaggt ccgcgggtga gctgatcgag gtatccgggt cgcgaccggc ccagatgtcg
     1681 gcgatcgcga cggcgtcggc acccgcgagc gcgtccgcga acgcgtccag cagcgccgcg
     1741 gtccggtgat acgtgagcgg ctcgtagacc gcccacaccc gcctgccggg ctcgcgctgg
     1801 cgcacggccg caagggtctc gcggatcgcc gtcgggtgat gcccgtaatc gtcgtagacc
     1861 acgacaccgt gagcctcgcc cttgcgctcg aggcgcctcc ccaccccacc aaacccggcc
     1921 agcgcggcga tcacggccgg ggcagggacg cctactgccg ccgccgcgcc cgcgacaccg
     1981 agcgcgttcg ccgcgttgtg gcggccggcg gtgggcagcc ggaccggcag cgggcccgcc
     2041 agtgggtcca gtcccgcgat ctcgagcacc gtcgcgtctg gctccgccga ggtgatccgg
     2101 ccgagcaggg tgtcggcggg acccgacgcg ctgcggaacc gctcggcgat cccgcgcgcg
     2161 aagccgccaa gccgttgcgg cgcaacgtcc acgagcgcgg tcgcgaggat ccgccccggc
     2221 cagtcggcaa gcctgtcgac gagctcggcg acgccctcgt cgcccacgtt cgcgacgagg
     2281 cacgtcccgc tgtccccgcc ggtgggcgcg gcacgccgga tccacgcctc gaacgccgcg
     2341 agcacggcgc cacggtcggc gaagacgtcg gggtggtccc actcggcaga ggtcagcacg
     2401 atcaccgacg gccggtaggc atcgaagttg ccggcgtact cgtcggcctc gacgacgaag
     2461 acgtcgcccc cgccccggcg cgcggtggcg gggacgccaa gccccgtcag cgccgcgggc
     2521 aggagcgccc ccacgaacgc gcccgggtct cgaccagccg ccgcgagcac gtggaccagc
     2581 cagcccgcgg acgtgctctt tccgtgcgtc ccggcgatcc cgactagacg gcgaccagcg
     2641 gcggcgtccg ccacgacctg ttgccagggc tcgagcggga tgcccgccgc ccgcgcggcg
     2701 gcgagctcgg cgttgccggg gtcgatcgcg gtgagggcct tggtcaccgc gagccgc
//
LOCUS       DBNHXO010000480         2022 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4121466, whole genome shotgun sequence.
ACCESSION   DBNHXO010000480 DBNHXO010000000
VERSION     DBNHXO010000480.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4121466"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="AC162A_09740"
     CDS             <1..523
                     /locus_tag="AC162A_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014788.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HIR5011167.1"
                     /translation="LLLTKRLGTGFLVSGRRSGRTSDADLAAAIAQMRTLNLASSEVL
                     ARHGIAGATDVTGFGLLGHGLEMARASGTRLVFDAAALPALDGSLDLARAGIETGGAG
                     HNRRYTAPALTVAAGVAPELVALAHDPQTSGGLLAAVSPDQATSIAADLEAAGAGAWV
                     VGRVEAGEGVALA"
     gene            complement(528..1112)
                     /locus_tag="AC162A_09745"
     CDS             complement(528..1112)
                     /locus_tag="AC162A_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5011168.1"
                     /translation="MLRIAVRLLAAITILAALGAGSVAPAVAGGGCHGPDGTVHTEGD
                     ATVVRMDTCAFAPTVVHVPVGTEVRFLNTAQVDHLVVGEAQSWGTDDVLAPGQDTSRR
                     FATKGVFPFSCPLHPGMVGAVVVGGAGAAAVLPPAAGGAGSDAAPAASSTTPVSDGPS
                     ILPIVGVAILAAAILVGGGLIARRVGRRTAVRES"
     gene            1222..>2022
                     /locus_tag="AC162A_09750"
     CDS             1222..>2022
                     /locus_tag="AC162A_09750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5011169.1"
                     /translation="MSIAGTYRQLLRNGPLTRLFVGEFVSSIGDWLYLVALVVLVYQE
                     TQNPVVLGIVGAARMLPYILLSIPAGIISDRFDRRLVLLTTDLARAACMLVMAWIVAT
                     GGPLWAVAAVAILAACFSTFFYPAIGALIPSLVRDEREFGPANSAWATLDNMAWIVGP
                     GIAGLLLATGDLVLAFVLNAVSFTLIAVILWTLPRSSPGAASSAGPASSEDSTAAVDA
                     ATEMPEPEGRVFLGRRVPASINLSALAGVVLIDAFTWFTFGGISILIVV"
BASE COUNT          261 a          760 c          710 g          291 t
ORIGIN      
        1 cctgctcctg accaagcgtc tcggcaccgg gttcctcgtc agcggccgac gatccggccg
       61 aacctccgac gccgacctcg cggcggccat cgcccagatg cggaccctca acctcgcgtc
      121 gtccgaggtc ctcgcgcggc acgggatcgc cggggcgacc gacgtgacgg ggttcgggct
      181 cctggggcac ggcctggaga tggcccgggc ctcgggcacg cggctggtgt tcgatgccgc
      241 cgcgctgccg gccctcgacg gctcgctcga cctcgcccgg gccggaatcg agaccggcgg
      301 cgccggccac aaccgccgct acacggcgcc tgcgctcacc gtggcagccg gcgtcgcgcc
      361 cgagctggtc gccctcgccc acgacccgca gacgtccggc ggcctgctcg ccgccgtctc
      421 acccgatcag gcgacgtcga tcgcggcaga cctggaggcc gccggcgcgg gagcgtgggt
      481 cgtcgggcgc gtcgaggcag gcgaaggggt cgcgctggcc tgaccagcta ggactcgcga
      541 acggcggtgc gacggccgac gcgtcgcgcg atgagcccgc caccgacgag gatggcggcg
      601 gccaggatcg cgaccccgac gatcggcagg atcgacggcc catcggacac cggggtcgtc
      661 gaggatgcgg ctggagccgc atcgcttccg gcgcctccgg ccgccggggg cagcacggcc
      721 gccgcgcccg ccccgccgac cacgaccgca cccaccatcc ccgggtggag cgggcagctg
      781 aacgggaaga cgcccttcgt cgcgaaccgg cggctcgtgt cctggccggg cgcgagcacg
      841 tcgtccgtgc cccacgactg cgcctcgccc accacgaggt ggtcgacctg ggccgtgttg
      901 aggaaccgga cctccgtgcc gaccgggacg tgcacgaccg tcggcgcgaa cgcacaggtg
      961 tccatccgga cgaccgtcgc atcgccctcg gtgtggacgg tgccatcggg cccgtgacag
     1021 ccgccacccg cgacggcggg cgccacggag ccggctccga gcgcggcgag gatggtgatc
     1081 gcggcgagca gccgcacggc gattcgaagc atgacaggcg cctccgggat gggtcagtgt
     1141 gctttggata caccgcacgg acgccgtcgg ttcccgctcc cgcatgcgca acaatcctgc
     1201 gcaagggagc gagagggacc gatgtcgatc gcgggcacgt atcgccagct gctgcgcaac
     1261 gggccgctga cgcggctgtt cgtcggcgag ttcgtgtcct cgatcggcga ctggctctac
     1321 ctcgtcgccc tcgtcgtgct cgtctaccag gagacgcaga acccggtcgt tctcggaatc
     1381 gtcggggcgg cgcggatgct gccgtacatc ctgctgtcga tcccggcggg gatcatcagc
     1441 gaccgcttcg accgtcgcct cgtcctcctg accacggacc tcgcgcgcgc cgcctgcatg
     1501 ctcgtgatgg cctggatcgt cgcgacgggc ggcccgctgt gggccgtcgc cgcggtcgcc
     1561 atcctggccg cctgcttctc gaccttcttc tacccggcga tcggcgccct gatacccagc
     1621 ctcgtccgcg acgagcgcga gttcgggccg gcgaactcgg cctgggcgac gctcgacaac
     1681 atggcctgga tcgtcgggcc cgggatcgcc ggtctgttgc ttgcgaccgg cgatctcgtg
     1741 ctcgcattcg tgctcaacgc cgtgtcgttc acgctgatcg ccgtgatcct gtggaccctg
     1801 ccgcggagca gcccgggcgc ggcgagcagc gccggaccgg cctcgtccga ggacagcacg
     1861 gccgccgtcg acgccgcgac cgaaatgccc gagcccgagg gtcgggtctt cctcggccgc
     1921 cgggtcccgg catcgatcaa cctgtcggcc ctcgctggcg tggtgctcat cgacgccttc
     1981 acctggttca ccttcggcgg catcagcatc ttgatcgtgg tc
//
LOCUS       DBNHXO010000481         4761 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5115335, whole genome shotgun sequence.
ACCESSION   DBNHXO010000481 DBNHXO010000000
VERSION     DBNHXO010000481.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5115335"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="AC162A_09755"
     CDS             <1..1281
                     /locus_tag="AC162A_09755"
                     /inference="COORDINATES: protein motif:HMM:NF023128.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5011170.1"
                     /translation="ANTMFSFAVVSGAIGLYLVNDNQFGERDGNVVLSLAVIVSVALN
                     AAVSPILGAFSDWGGRRMPFLLVFTALCIGATFFIADVPPVLGVALFIVANFAYQAAL
                     IYYDATLKTVSTPANRGRLSGIGTGIGYCGTVFIGLLIFLLDIPVADRFRLTAILFLV
                     FALPIFWFVRERRRPDERPITKADVADSFGQLKRSIAHSREVPGLWRFLIGRFFYSDA
                     VNTVIVVMSVVVVKTMGVSEATANVILLMLTFVAIVMSFVWGFLVDRNGPKLTLVWVL
                     ISWAVGLIIGGVAIGFGQAGLVPFLVAGAILGSGLGGVQVADRVLMVRLSPPDRIGEF
                     FGIYGLVGKGSQVVGQILYGALIFLLLDSFGNGAYQVAVLSLLVTMLIGLWLVWPVSD
                     QWSGSGELGGGPPTDADAPPPRLSPDRAPLSDRA"
     gene            complement(1357..3843)
                     /locus_tag="AC162A_09760"
     CDS             complement(1357..3843)
                     /locus_tag="AC162A_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HIR5011171.1"
                     /translation="MRTTENGGSRDGERGQIMIVFTLVVVLLMALAAIVIDVSLLRTD
                     GQKLQNALDAGALAAGHTLPVNNTTIAANKLVARDYTRVNYPGMPNANVPDPTAQYAC
                     LIGLDTATGLPRVADMPLVCNVSFGPTSFQWKCTTAVCWAPCDPVLHPTDVCNTVILT
                     SNVTQPYFFGRAVGVNSGNTGVITSAACTGVCGNLPVQPVDVVILLDRTGSMDDSSSV
                     DNLRSGARSALQAFDPALQRIALGFTGPTSPTRTTNGNYGNQNSSRPLNAAGNQITSA
                     SLRATACTPTSGYSNTVHAIAIDPDFRPAGPVPTFVTTYSNANASGGANTISIARPTT
                     PDAVNVGDFLLAAIAVDDGDDVTIQTPGGWTSLNRTNNDDDVGFQTFYRFATSADQAA
                     GNWTWNISSGTRAVATIVRYTGVNPADPIDGNSEDDGSTSFFNQNLQAQSIETDNANS
                     ALIGVFTANQRTSFTMANPTPLTEIADLSHPTVSSGPSMAIGRGVQANPDDTGDRNAT
                     IGSNGIQWATQLIGLNAVPGPADTYDQTDQDQWLPIGFTGLDTNTPAVTHREAYVDAS
                     NNPRPSTDIVQAIGCFDVSQVGTNLATPLDQARQYLASHGRRSSIPGIKQGIILETDG
                     QPEHNSGQEGVGNWRTWATCADSTRAANAVKNADIELFTIGYGLESRNCPDGGLSATR
                     QLASMATDSPLVNNFNCSNTTQRNAENTDGDHFFCTPEGGNLTDILHQAAVQLAGGTR
                     LVQMYPTPIVSGVSPNRTAIGNTVTITGKYFTEAYAVTFGGVNSGGFNVISDTVISAV
                     VPPGSGTVDVQVSTPGGTSIISSGDKFTYP"
     gene            complement(3855..4310)
                     /locus_tag="AC162A_09765"
     CDS             complement(3855..4310)
                     /locus_tag="AC162A_09765"
                     /inference="COORDINATES: protein motif:HMM:NF019428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HIR5011172.1"
                     /translation="MGMWSTALERRRRGRGRPRQRGQSLVEFALVFPLFILLLAGMID
                     FGLGLYSYMTINNATRDSARLGATMCTANPCAAAVSARAVNAASGLAIEPPVVACTTP
                     ANASVNCASGTIKPGDNVTVTITYNYKMIWPLGFGTVIPMTSSMKMLAD"
     gene            complement(4520..>4761)
                     /locus_tag="AC162A_09770"
     CDS             complement(4520..>4761)
                     /locus_tag="AC162A_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR5011173.1"
                     /translation="LGTSTGFSVREVLGVAGTIVGRPIPHAVGPRRAGDPPVLVASNA
                     RAREVLGWTPRRSTLETMIGSAWDWRRSHPGGYPD"
BASE COUNT          663 a         1480 c         1738 g          880 t
ORIGIN      
        1 gcgaacacga tgttctcgtt cgccgtcgtg tccggtgcga tcgggctgta cctggtcaac
       61 gacaaccagt tcggggagcg cgacggcaac gtcgtcctgt cgctcgccgt gatcgtcagc
      121 gtcgccctca acgccgccgt gtcgccgatc ctgggcgcgt tctccgactg gggtggcagg
      181 cggatgccct tcctgctcgt cttcaccgcg ctgtgcatcg gcgccacgtt cttcatcgcg
      241 gacgtgccgc cggtccttgg cgtcgccctg ttcatcgtcg ccaacttcgc ctaccaggcc
      301 gcgctgatct actacgacgc gacgctcaag accgtgagca cgcccgcgaa ccgcggcagg
      361 ctgtccggga tcggcacggg catcggctat tgcgggacgg tattcatcgg cctgctgatc
      421 ttcctgctcg atatcccggt cgcggaccgg ttccgcctga cggcgatcct gttcctcgtc
      481 ttcgcgctcc cgatcttctg gttcgtgcgc gagcggcgcc gcccggacga gcgcccgatc
      541 acgaaggcgg acgtcgcgga ttcgttcggg cagctcaagc gctcgatcgc ccactcgcgc
      601 gaggtgcccg ggctgtggcg gttcctgatc ggccgcttct tctactcgga cgctgtcaac
      661 accgtcatcg tcgtgatgag cgtggtcgtg gtgaagacga tgggcgtgtc cgaggccacg
      721 gccaacgtga tcctgctgat gctgacgttc gtcgccatcg tgatgagctt cgtctggggc
      781 ttcctcgtcg atcgcaacgg gccaaagctg acgctggtgt gggtgctcat ctcgtgggcc
      841 gtgggcctga tcatcggcgg ggtcgcgatc ggcttcgggc aggcgggcct ggtcccgttc
      901 ctggtcgccg gcgccatcct cggcagcggc ctgggtggcg tccaggtcgc ggatcgggtc
      961 ctgatggtcc gcctgtcgcc gcccgaccgg atcggcgagt tcttcgggat ctatgggctg
     1021 gtcggcaagg gatcgcaggt ggtgggccag atcctctacg gcgcgctcat cttcctgctg
     1081 ctggacagct tcggcaacgg cgcctaccag gtggccgtgc tgagcctgct ggtcaccatg
     1141 ctcatcgggc tgtggctggt atggccggtc agcgaccagt ggtcggggtc aggcgagctc
     1201 ggcggcggtc cgcccacgga cgccgacgcg ccgccgccgc ggctgtcgcc cgatcgcgcg
     1261 ccgctcagcg atcgcgcgta gccggggcaa cgaaggacgg ctgccgggga ccggcagccg
     1321 tcatgtggtg ctcaggatcg gcgaacgacg ccgggcttat gggtaggtga acttgtcacc
     1381 gctgctgatg atcgacgtgc caccgggtgt cgagacctgg acgtcgaccg tgccggagcc
     1441 gggcgggacg acggcgctga tcaccgtgtc gctgatgacg ttgaacccgc cggagttgac
     1501 cccaccgaac gtgacggcat aagcctccgt gaagtacttg cccgtgatcg tcaccgtatt
     1561 gccgatggcg gtccggttcg gtgagacgcc gctgacgatc ggggtcgggt acatctggac
     1621 gaggcgcgtg ccgccggcga gctgaaccgc cgcctggtgg aggatgtccg tgaggttgcc
     1681 gccctctggc gtgcagaaga agtggtcgcc atcggtgttc tcagcgttcc gctgcgtcgt
     1741 gttgctgcag ttgaagttgt tgaccagggg actgtcggta gccatcgacg cgagctgcct
     1801 ggtagccgac aggccgccgt ccgggcagtt ccggctttcg agcccatagc cgatcgtgaa
     1861 cagctcgatg tcggcgttct tgacggcgtt ggccgccctg gtggagtcgg cgcacgtcgc
     1921 ccacgtccgc cagttgccca cgccttcctg gccgctgttg tgttccggct ggccgtcggt
     1981 ctcgaggatg atgccctgct tgatgcccgg gatggaactc ctgcggccgt gggaggcgag
     2041 gtactgccgg gcctggtcga gaggcgtggc caggttggtg ccgacctgcg agacgtcgaa
     2101 gcagccgatg gcctgcacga tgtccgtgct cggacgcggg ttgttgctgg cgtccacgta
     2161 cgcctcgcga tgggtcacgg ccggggtatt ggtgtccagg cccgtgaacc cgatcgggag
     2221 ccactggtcc tgatccgtct ggtcgtaggt gtcggccggg cctgggacgg cgttgaggcc
     2281 gatgagctgg gtcgcccact ggatgccgtt ggagccgatg gtcgcgttgc ggtcgccggt
     2341 gtcgtccggg ttcgcctgga cgccgcggcc aatggccatc gacgggccag agctgacggt
     2401 cggatgcgag aggtcggcaa tctcggtcag cggggtggga ttggccatcg tgaacgaggt
     2461 ccgctggttg gcggtgaaca cgccgatcag ggcggagttg gcgttgtccg tctcgatcga
     2521 ctgggcctgc aagttctgat tgaagaagct cgtcgaaccg tcgtcctcgc tgttcccatc
     2581 gatcggatcg gcgggattga cgcccgtgta gcgcacgatg gtggcgaccg ctcgggtgcc
     2641 gctggagatg ttccaggtcc agttgccggc ggcctggtcg gcactggtcg cgaacctgta
     2701 gaaggtctgg aagccgacgt cgtcgtcgtt gttggtccgg ttgagggacg tccacccccc
     2761 tggcgtctgg atggtcacat cgtcgccgtc gtcgaccgcg attgcggcga gcaggaagtc
     2821 gccgacattg acggcgtcag gcgtggtcgg cctggcgatc gagatcgtgt tcgcgccacc
     2881 cgacgcgttg gcgttcgagt aggtggtgac gaaggtcggc accgggccgg cagggcggaa
     2941 gtccgggtcg atcgcgatcg cgtggacggt gttcgagtag ccgctggttg gcgtgcaggc
     3001 cgtggcccgc agcgaggcgc tggtgatctg gttgcccgcc gcgttgagcg gcctggacga
     3061 gttctggttg ccgtagttgc cgttcgtcgt ccgggtcggg gacgtgggtc ccgtgaaccc
     3121 gagagcgatg cgctggagcg cgggatcgaa cgcctggagg gccgagcggg ccccggagcg
     3181 aaggttgtcg acggaggacg agtcgtccat gctgccggtg cggtcgagca ggatcacgac
     3241 gtcgacgggc tggaccggca ggttgccgca caccccggta caggcagcag acgtgatcac
     3301 accggtgttg ccgctgttga cgccgaccgc gcgcccgaag aagtagggct gcgtcacgtt
     3361 ggacgtcaga atgaccgtgt tgcagacgtc ggtcgggtgc agcaccggat cgcagggcgc
     3421 ccagcacact gcggtcgtgc acttccattg gaagctcgtc gggccgaagg agacgttgca
     3481 caccaggggc atgtccgcga cgcggggcag cccggttgcc gtgtcgaggc cgatgagaca
     3541 ggcgtactgc gccgtcgggt ccggcacgtt cgcgttgggc atgccggggt agttaacgcg
     3601 cgtgtagtcg cgtgcgacca acttgttggc cgcgatggtg gtgttgttca ccggcagcgt
     3661 gtggcccgcg gccagcgcgc ccgcgtcgag ggcgttctgg agcttctggc cgtccgtgcg
     3721 cagcaggctg acgtcgatca cgatcgcggc cagggccatc agcaggacga ccacgagcgt
     3781 gaacacgatc atgatctggc cgcgctcacc gtcgcgggag ccgccgttct cagtggttct
     3841 catgttccta cccatcagtc ggccagcatc ttcatgctcg aggtcatcgg gatcaccgtg
     3901 ccgaatccga gcggccagat catcttgtag ttgtaggtga tcgtgaccgt cacgttgtca
     3961 ccgggcttga tcgtgcccga cgcgcagttg accgaggcgt tggcgggcgt ggtgcacgcg
     4021 acgaccggcg gctcgatggc gaggccgctc gccgcgttga ccgcgcgggc cgagacggca
     4081 gcggcgcacg ggttcgcggt gcacatcgtc gcgcccagcc gcgccgaatc gcgggtggcg
     4141 ttgttgatgg tcatgtagga gtagaggccc aggccgaagt cgatcatgcc cgcgagcagc
     4201 aggatgaaca gcggaaagac gagagcgaac tcgaccagcg actggccgcg ctgcctggga
     4261 cgcccccggc cgcgacgacg gcgttcaagc gcggtgctcc acatgcccat ggacgtgtcc
     4321 ctcgtgatga tctgacggga tgaatcggtg ttgacggcgc ccggagtcgt ggcggatcgc
     4381 cagcagacat gacggacctt gcggcgttcc ttacaggact atggtcctgg tactcaatcc
     4441 gcgcaatctg cgcgagttcc gttgctggcg gtcaccagaa cggccgccgt cgaggcgtcc
     4501 gcgacccgac gaggcgccgt cagtccgggt agcccccggg atggctgcgg cgccagtccc
     4561 aggccgatcc gatcatggtt tcgagcgtgg atcgcctcgg tgtccagccc agcacctcgc
     4621 gcgcgcgggc atttgaggcg acgagcacgg gcggatcgcc ggcgcggcgc ggacccacgg
     4681 cgtgcgggat cgggcggccg acgatggtcc cggcgacgcc caggacctcg cggaccgaga
     4741 agccggtcga tgtgccgaga t
//
LOCUS       DBNHXO010000482         2244 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2932120, whole genome shotgun sequence.
ACCESSION   DBNHXO010000482 DBNHXO010000000
VERSION     DBNHXO010000482.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2932120"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="AC162A_09775"
     CDS             complement(<1..647)
                     /locus_tag="AC162A_09775"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5011174.1"
                     /translation="MTPRPILVIAALTLREIVRRRVIWVLLALVIVSVALVGWGVERL
                     VTLAREGDVSEIEIRIGVSQVLILIAFMFSFVLAMSAAFLAAPAIASDVETATVHAML
                     ARPMRRSDLVIGRWLGLSIVVGGYATLASLLAMAVVNLVSGHMPPEPLVAVAFLAFQA
                     VTVLTLALALGTRLPAIAAGAVTVVLFGVGWFVGVLGSVAILFEAAPLARAADVMR"
     gene            complement(644..1600)
                     /locus_tag="AC162A_09780"
     CDS             complement(644..1600)
                     /locus_tag="AC162A_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5011175.1"
                     /translation="MTVPAIETHGLRKVYGRRTVGLESLDLAVGAGEVFGFLGPNGAG
                     KTTAVKLLLGLTRPSAGEGSVLGRPIGDRATRSRVGYLPELFRYQAWLTAREVLELHA
                     QLAGLPAKGRRAEIDRVLALAGLADRSEDRTGGFSKGMQQRLGLATALLGDPALVILD
                     EPTSALDPVGRDDVRAIIREARDRGSAVFLNSHLLGEVERLCDRVAIVNHGRVIAAGR
                     LTELLGESAVRIQVTHLPPGPAVAAALGPFGTVAHDDEWLTIHPIDPDRIPDAVAAVV
                     GLGGRVHAVDPGRRSLEDLFLGMVRDDGAAPDEVAAAGTRPA"
     gene            1686..1850
                     /locus_tag="AC162A_09785"
     CDS             1686..1850
                     /locus_tag="AC162A_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011176.1"
                     /translation="MNTSPEAHPMLLVDCPLCDGPAPFDAVEDTLDCDACGVVLEVAT
                     PEPVALPAAA"
     gene            1957..>2244
                     /locus_tag="AC162A_09790"
     CDS             1957..>2244
                     /locus_tag="AC162A_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012455.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HIR5011177.1"
                     /translation="MDFRKYSAELIGSFFFFVIGYLSIPAFANASAPGAPNLLVVPWA
                     FGLGLLAAIFAFGHISGGHFNPAVTIAMVLDKRTTPMDAVGYILSQIIGAIA"
BASE COUNT          347 a          795 c          798 g          304 t
ORIGIN      
        1 cgcatcacgt cggccgcacg ggcgagcgga gccgcctcga acaggatcgc gacgctgccc
       61 aggaccccga cgaaccagcc cacgccgaac agcaccacgg tgaccgcacc ggcggcgatc
      121 gccggcagcc gggtcccgag ggcgagcgcg agcgtgagca cggtgactgc ctggaacgcg
      181 aggaaggcga cagcgaccag cggctcgggc ggcatgtggc cgctgacgag gttgacgacc
      241 gccatcgcga gcaggctcgc aagggtcgcg tagccaccga ccacgatcga cagcccgagc
      301 cagcggccga tcacgaggtc gctgcgacgc atcggccgag ccagcatcgc gtgcacggtg
      361 gcggtctcga cgtccgatgc gatcgccggc gcggccagga acgcggccga catcgcgagc
      421 acgaagctga acatgaacgc gatcaggatc agcacctgcg agacgccgat ccggatctcg
      481 atctcgctca cgtcgccctc gcgggcgagg gtcaccagcc gttcaacgcc ccagccgacg
      541 agcgcgacgc tcacgatcac gagcgcgagc aggacccaga tcactcgccg gcgcacgatc
      601 tcgcgcaggg tgagggcggc gatgacgagg atcgggcgcg gggtcatgcc ggccgcgtcc
      661 cggcggccgc gacctcgtcg ggcgccgccc cgtcgtcgcg gaccatgccc aggaacagat
      721 cctccaggct gcgacggccg ggatcgacgg cgtggacccg gccaccgagg ccgacgacgg
      781 cggcgacggc atcggggatc cggtccgggt cgatggggtg gatcgtgagc cactcgtcgt
      841 cgtgggcgac ggtgccaaac ggtccgaggg cggcggcgac cgcgggaccg gggggcagat
      901 gggtgacctg gatgcgcacg gccgattcgc ccaggagctc ggtcagcctc ccagctgcga
      961 tcactcgacc gtggttcacg atcgcgaccc ggtcgcacag ccgctcgacc tcgcccagga
     1021 ggtgcgagtt gaggaacacc gccgagccgc ggtcgcgcgc ctcccggatg atcgcgcgca
     1081 cgtcgtcgcg gccgacgggg tcgagcgccg aggtgggttc gtccaggatg accagagccg
     1141 ggtcgccgag gagcgcggtc gcgaggccga ggcgctgctg catgcccttg gagaaaccgc
     1201 cggtccggtc ttcggatcgg tcggcgagcc cggcaagtgc gagcacacga tcgatctcgg
     1261 cgcgccgccc cttcgcgggc agtccggcga gctgggcatg gagctcgagg acctcgcgag
     1321 ccgtcagcca ggcctggtat cggaacagct ccggcaggta gccgacgcgg cttcgcgtgg
     1381 cgcggtcgcc gatcgggcgc ccgaggacgc tgccctcgcc ggcgctcggg cgggtgaggc
     1441 cgagcagcag cttgaccgcg gtcgtcttgc ccgccccgtt aggcccgagg aacccgaaca
     1501 cctcgcccgc gccgacagcg aggtcgagcg actcgaggcc aacggtcctg cggccgtaga
     1561 ccttgcgcag gccgtgggtc tcgatggcgg ggacggtcat ccggtcattc tcacgggtgt
     1621 tcgggtttgg cgccccgcgt cgtgacacga gagctgtcac agcccgcccc gacgatgcgc
     1681 ccgtgatgaa caccagcccg gaggcccacc cgatgctcct cgtcgattgc ccgctgtgcg
     1741 acgggccggc gccgttcgac gcggtcgagg acaccctcga ctgcgacgcg tgcggcgtcg
     1801 tgctcgaggt cgcgaccccc gaaccggttg cgctgcccgc ggccgcctga cccgccacca
     1861 gcggattctc tccctcgcca ctggcgttag gcgggatact tgccgaactg aaccggtgcg
     1921 cccaatgcgc cggcgccacg ggaggttgaa ctgccgatgg atttccgcaa gtactcagcc
     1981 gaactgatcg ggtcgttctt cttcttcgtg atcgggtacc tctcgatccc ggcgttcgcg
     2041 aacgcgagcg cacctggtgc tccaaacctg ctggtcgtgc cctgggcctt cggtctcgga
     2101 ctgctcgccg cgatcttcgc gttcggccac atctcgggtg gccacttcaa ccctgccgtg
     2161 accatcgcca tggtgctcga caagcggacg acgccgatgg atgccgtcgg ctacatcctg
     2221 tcccagatca tcggggcgat cgcg
//
LOCUS       DBNHXO010000483         4201 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2337925, whole genome shotgun sequence.
ACCESSION   DBNHXO010000483 DBNHXO010000000
VERSION     DBNHXO010000483.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2337925"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="AC162A_09795"
     CDS             <1..602
                     /locus_tag="AC162A_09795"
                     /inference="COORDINATES: protein motif:HMM:NF025229.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR5011178.1"
                     /translation="VVDRTGGFLRWSRNFFPQDADSLAPTSALTWQLARDARISLRLY
                     DASGTLVRTVWTGKAQRAGDRTWTWNGRLKDGSFAPQGRYEARLTVASTLGTVALSRT
                     VWASAFSVTPSATKVRPGQTLKVTFTTLEPLTSRLAVAFRQPGRSAVTLTATKRKDGS
                     YTASFKVRSGGSGTGSIRVSAKDAGGGLNRTSVAIRVAS"
     gene            669..1457
                     /locus_tag="AC162A_09800"
     CDS             669..1457
                     /locus_tag="AC162A_09800"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HIR5011179.1"
                     /translation="MTTDHDAAAPTSAQPIGSGAWVILPTYNEADNLPGISAAILESL
                     PGAVLLVVDDGSPDGTGALADGLAQADARVRVKHRAAKQGLGRAYLDGFGEALTGGAT
                     MIVQMDADWSHDPLALPALIAPIVDGKADLVIGSRYTKGGGVEDWGIIRRVISRGGST
                     FARIVLSLKPHDLTGGFKAWRATTLASVPFDGVRAGGYVFQIEMTYRASRLGARVAEV
                     PITFRDRRAGQSKMSRRILVEALIVVVSLRWDELRGRGPVPRAK"
     gene            1454..2734
                     /locus_tag="AC162A_09805"
     CDS             1454..2734
                     /locus_tag="AC162A_09805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5011180.1"
                     /translation="MTAHDRPGWRPGTDHAAALAAPGVRVVMDLRPLQDPDRAPLTAG
                     YLEALLGALNDDPQPEESFSFLLAADLDDPSTRWPNLTVVGTRHLPPTRLLRSGALTV
                     DPFVLRGASIGAGWRAEREGAAGTVYHAAAGGLPIASGIPVVASLLDLAPWSMPEAYQ
                     SGTVARFGQRLRSRLFRDAAAVLVPSRATAAEARRLLRVRKARLRVVPLAARAAYRPE
                     AAAGAARERERLGLGERYAVYAGRYDARQDLPTLFAALASLAAEDRPATVEEGSWPPR
                     VCLVGATPGDRAAVSRAAVRAGVADSIAYAPRLPEGRLAALVAGARFLVQPVRSEASG
                     IAALDALAAAVPVVASSIGALPEVVGAAGILVEPGDPARLATAIRAVWADDALHEGLV
                     AAARERTEGARTWADVARETRAVWAEVARPAPIL"
     gene            complement(2736..4004)
                     /locus_tag="AC162A_09810"
     CDS             complement(2736..4004)
                     /locus_tag="AC162A_09810"
                     /inference="COORDINATES: protein motif:HMM:NF022702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HIR5011181.1"
                     /translation="MSRSATGAIVVVVLVVGALVGIGAASMLTATPGATSTPIAGVTS
                     PSPGAATSSPGGSPAPTEPGGSSAEPPPSTEPSASPSPTPVPTPVTAPAPLTGMPVKP
                     AQAERRVVAVMIDDQFDARPQSGLSDADVVWQAPAEGGIPRYMAFFQQGDPPAVGPVR
                     SSRLYFIAWASEWRSLYVHAGGSPQAKALLASSKGQGSYVYNADEFRWGGGRYLWRIK
                     TRFAPHNVYTDGKSLRRLARRVGADPVAGQKPIWKFGDPAPIEERPEGGTIAVPYLAN
                     KITYKYNRTTNRYQRSVSGEGKQVDAGDKDRVAPTNVIVMAVSFAPLNDGSHKSRLEA
                     QVTGKGKAWIATNGKTIRGTWKKPGFRAKTRFFDRNGDPVTLTRGQTFVQVVPRSATI
                     TVKDGKVPAPTAGVVVGAGGLYGTLGRQIS"
BASE COUNT          507 a         1546 c         1560 g          588 t
ORIGIN      
        1 tggtcgtcga ccgcaccggc ggcttcctgc gctggtcgcg caacttcttc ccacaggacg
       61 ctgacagcct cgcacccacc tcggccctca cctggcagct ggcgcgggac gccaggatct
      121 cgctccgcct gtacgacgcg tccggaacgc tggtccggac cgtctggacg ggcaaggcgc
      181 agcgtgcggg cgaccgcaca tggacctgga acggacgcct caaggacggg tcgttcgcgc
      241 cgcagggtcg ctacgaagcg cggctgacgg tcgcctcgac cctgggcacg gtcgccctct
      301 ctcggaccgt gtgggcaagc gcgttctccg tgacgccgtc cgcgacgaag gtgcgccccg
      361 gccagacgtt gaaggtcacc ttcacgaccc tcgagccgct gacctccagg ctggccgtcg
      421 cgttccgcca gcccggccgg tccgccgtca ccctgaccgc gacgaagcgc aaggacggct
      481 cgtacaccgc ctcgttcaag gtgcggagcg gcggctcggg gaccggctcg atccgggtct
      541 cggcgaagga cgccggcgga gggctcaacc ggacgtcggt cgcgatcagg gtcgcgtcgt
      601 gagcctccga cctcgacccg gcgcccccgt cggcccgtac actctcgccg catgacgacc
      661 gaaccgcgat gacgaccgac cacgacgcag ccgcaccgac gagcgcgcag cccatcgggt
      721 ccggcgcctg ggtcatcctg ccgacctaca acgaggcgga caacctgccc gggatcagcg
      781 cggcgatcct cgagtcgttg ccgggggccg tgctcctggt cgtggacgac ggctcgcccg
      841 acgggaccgg ggcgctcgcc gacggcctcg cccaggccga tgcgcgcgtt cgtgtcaagc
      901 atcgggccgc caagcagggc ctcggccgtg catacctcga cgggttcggc gaggccctca
      961 ccggcggcgc cacgatgatc gtccagatgg acgccgactg gtcgcacgac ccgctggccc
     1021 tgccggcgct gatcgcgccg atcgtggatg gcaaggccga tctggtgatc gggtcgcgct
     1081 atacgaaggg cggcggcgtc gaggactggg gcatcatccg ccgggtcatc tcgcgcggcg
     1141 ggtcaacgtt cgcgcggatc gtcctcagcc tcaagccgca cgacctcacc ggcggcttca
     1201 aggcgtggcg ggcgacgacc ctcgcgagcg tgccgttcga cggcgtccgc gcgggcggct
     1261 acgtgttcca gatcgagatg acctaccggg cgtcgcgcct cggggcccgc gtcgccgagg
     1321 tcccgatcac gttccgggat cgccgcgcgg gccagtccaa gatgagccgg cggatcctcg
     1381 tcgaggctct catcgtcgtc gtgagcctgc gctgggacga gcttcgcggt cgcgggccgg
     1441 tgccccgggc gaagtgaccg cgcacgaccg cccgggctgg cggccgggca cggaccacgc
     1501 cgcggctctc gcggcgccgg gggttcgcgt cgtcatggat ctccggccgc tgcaggaccc
     1561 cgaccgggcg ccgctcacgg cgggctacct cgaggcgctg ctcggggcgc tgaacgacga
     1621 cccgcagccc gaggagtcgt tctcgttcct gctcgccgcg gacctcgacg atccgtcgac
     1681 gcgctggccc aacctgaccg tcgtcggcac gcgccacctg ccgccgacgc gcctcctgcg
     1741 ctccggcgcg ttgaccgtcg atccgttcgt ccttcgcggc gcctcgatcg gcgcgggctg
     1801 gcgagcggag cgcgaagggg cggcagggac cgtctaccac gccgccgcgg gcgggctccc
     1861 gatcgcgtcg gggatccccg tggtcgcatc gctgctggac ctcgcgccat ggtcgatgcc
     1921 ggaggcctac cagtcgggca cggtcgcgag gttcgggcag cgcctgcggt cgcgcctgtt
     1981 cagggacgcc gccgcggtgc tggtcccgag ccgggcgacc gccgccgagg cgcgccgcct
     2041 gctccgggtg cgcaaggcga ggctgcgggt cgtgccgctc gccgcgcggg cggcgtatcg
     2101 acccgaggcg gccgcggggg cggcgcggga gcgcgagcgc ctcgggctgg gcgagcgcta
     2161 cgcggtgtat gcgggccgct acgacgcccg ccaggacctg ccgacgctgt tcgccgcgct
     2221 cgcgtcgctc gcggccgagg accggccggc aaccgtcgag gagggctcgt ggccgccgcg
     2281 ggtgtgcctg gtgggcgcga cgccgggcga ccgggccgcg gtgtcgcggg ccgccgtccg
     2341 cgcgggcgtc gccgactcga tcgcctacgc cccgcgcctg cccgaggggc gcctcgccgc
     2401 gctcgtggcc ggggcgcggt tcctcgtcca gcccgtgcgc tcggaggcgt cggggatcgc
     2461 cgcccttgac gccctcgcgg cggccgtgcc cgtggttgcg agctcgatcg gcgcgctccc
     2521 cgaggtggtc ggcgccgcgg ggatcctcgt ggagcccggc gatccggccc ggctcgccac
     2581 cgcgatccgg gccgtgtggg cggatgacgc actgcacgaa gggctcgtcg ccgccgcgcg
     2641 cgagcgaacc gagggtgcgc ggacctgggc cgacgtcgcc cgcgagacgc gtgccgtgtg
     2701 ggcggaggtc gcccggcccg cgccgatctt gtgacctagg agatctgtcg cccgagcgtg
     2761 ccgtacaggc cgcccgcgcc cacgacgacc cccgcggttg gagccggcac cttgccgtcc
     2821 ttgaccgtga tcgtggcgct ccggggtacg acctggacga acgtctggcc tcgcgtcagc
     2881 gtcaccgggt cgccgttgcg gtcgaagaag cgggtcttcg cccggaaccc cggcttcttc
     2941 caggtgccgc ggatcgtctt cccgttggtc gcgatccagg ccttgccctt gcccgtgacc
     3001 tgcgcctcga gccggctctt gtgactcccg tcgttgagcg gcgcgaagct cacggccatc
     3061 acgatcacgt tcgtcggggc gacgcggtcc ttgtcgcccg cgtcgacctg cttgccctcg
     3121 ccgctcaccg accgctggta gcggttcgtg gtgcggttgt acttgtacgt gatcttgttg
     3181 gcgaggtagg gcacggcgat ggtgccgccc tcgggccgct cctcgatcgg cgcggggtca
     3241 ccgaacttcc agatgggctt ctggcccgcg accggatcgg caccgaccct tcgcgcgagc
     3301 cggcgcaggc tcttgccgtc ggtgtacacg ttgtgggggg cgaagcgcgt cttgatccgc
     3361 cacaggtagc ggccgccgcc ccagcggaac tcgtcggcgt tgtacacgta ggagccctgt
     3421 cccttggacg aggcgagcag ggccttcgcc tgcggcgagc cgcccgcatg gacgtacagc
     3481 gaccgccact cggaagccca ggcgatgaag tacagccgcg aactgcgcac aggcccgacg
     3541 gcgggcgggt cgccctgctg gaagaaggcc atgtagcgcg ggatgccgcc ctcggcgggc
     3601 gcctgccata cgacgtcggc atcggacagc ccggactgcg gccgggcgtc gaactggtcg
     3661 tcgatcatca ccgccaccac gcggcgctcg gcctgcgcgg gcttgacggg catgccggtg
     3721 agcggggccg gagcggtgac gggcgtcggg accggggtcg gcgacggcga tgccgacggc
     3781 tccgtgctgg ggggcggctc agcgctcgag ccgccgggct ccgtcggcgc aggcgagccg
     3841 ccgggcgacg acgttgcggc gccgggcgaa ggcgacgtga cgcccgcgat cggtgtggac
     3901 gtcgcgccag gcgtggcggt cagcatgctc gcggcaccga tgccgacgag cgcgccgacg
     3961 acgaggacca cgacgacgat ggcgccggtt gcgctccggc tcacgaggcg gaccctcgct
     4021 gacgacgttg tcggcggttc atgcacttcc ccggccgcgg ttctcggcca cgcgaccccg
     4081 cgtcggctac aaggccagac ccggtcgggc gcgaggatag ccgaacttgt cgcgacatgc
     4141 cctccgcggg cgcccgacac cgacaacgtg gccgtccacc accgttgggg taggctgcgc
     4201 c
//
LOCUS       DBNHXO010000484         2852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2338104, whole genome shotgun sequence.
ACCESSION   DBNHXO010000484 DBNHXO010000000
VERSION     DBNHXO010000484.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2338104"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1471)
                     /locus_tag="AC162A_09815"
     CDS             complement(<1..1471)
                     /locus_tag="AC162A_09815"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018852936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1211..1213),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HIR5011182.1"
                     /translation="MPKEYVRLTTPLVRDGDRQTGVLRPASWDEALERAAAGLRAAGA
                     AHGSGAYGVFSCSKSTNEVNFAAQKFSRAVLGSNNIDSCNRTUHAPSVAGLATVFGAG
                     GGTSSYQEAEETDCFLLWGSNARETHPIFFHHLLKGVRRGARLIAVDPRRTSSAQWAD
                     LWLGLDVGSDIALANAMAREIIHAGLANSEFIARATTGFEAYRAGVEPYTLEYAERET
                     GVPAAAIREAAHAYATADRAMVCWTLGITEHHNAVDNVLALISLALLTGHVGKYGSGI
                     NPLRGQNNVQGGGDMGALPDRLVGFQHVENDELRDKCDTLWGVRVPPKKGWHLSNMFD
                     AMERGDLTALYVIGENPMQSEADRHRTEKLLRGLDTLIVQDIFLTATGEIADVVFPAA
                     AAWAETLGTVTNSERRVQLVRPALEPPGEARDDLRIIFDLANALGAGWGEADAETVWN
                     EVRELSPVHRGMSYARLEALGGIQWPCWDEDHPGELFLHS"
     gene            complement(1525..>2852)
                     /locus_tag="AC162A_09820"
     CDS             complement(1525..>2852)
                     /locus_tag="AC162A_09820"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5011183.1"
                     /translation="LIPSLVRDEREFGPANSAWATLDNMAWIVGPGIAGVLLATGELV
                     LAFVLNAVSFTLIAVILWTLPRSSPGAASSAGPAPSEESAAALDAATETPEPEGRVFL
                     GRRVPASINLSALAGVVLIDAFTWFTFGGISILIVVIAVDVFHGGDAATGYLNAAIGI
                     GGTIGALLSGALVLRPRLGGVLLVGAAAFAVATVILGLANQLAVAFLAIAVISAGNLI
                     LDVTRTTIFQRVVPDEYRGRFGGLMMTSQTLSEAGGTLIVPILVSLFGFGIVMGFAGV
                     GVAAATILAVALIAGAGDVPIGEHDLALRRIARLSLFGGLPPARVEGALRRLEPLAVH
                     AGDVIVREGDPADRFYVIDTGSFDVTQADTAGGEPRHLRTLGTDEVFGERGLIGQTPR
                     TATVTAATDGLLFEMDGDDFLHLVGARKGVADRLMALYDPPTAEVVARG"
BASE COUNT          422 a         1005 c         1025 g          400 t
ORIGIN      
        1 gcgagtggag gaacagctcg ccagggtggt cctcgtccca gcacggccac tggatcccgc
       61 cgagggcctc gagccgcgcg tagctcatcc cgcgatgcac gggcgacagc tcccggacct
      121 cgttccacac cgtttccgcg tctgcctcgc cccagccggc gccgagagcg ttggcgaggt
      181 cgaagatgat cctcaggtcg tcgcgcgcct cgcccggcgg ctcgagcgcc gggcgcacca
      241 gctgcacccg ccgctccgag ttggtcacgg tgcccagggt ctccgcccat gccgccgccg
      301 cggggaacac gacgtcggcg atctcgcccg ttgccgtcag gaagatgtcc tggacgatca
      361 gcgtgtccag gccgcgcagc agcttctcgg tgcggtgccg atcggcctcg gactgcatcg
      421 ggttctcgcc gatcacgtac agcgcggtga gatcgccgcg ctccatcgcg tcgaacatgt
      481 tcgacaggtg ccagcccttc ttcggcggga cccggacgcc ccagagcgtg tcgcacttgt
      541 cgcggagctc atcgttctcg acatgctgga acccgaccag gcgatcgggc agggcgccca
      601 tgtcgccgcc gccctggacg ttgttctggc cgcgaagcgg gttgatgccg ctgccgtact
      661 tcccgacgtg ccccgtcagc agcgccagcg agatcaaggc gagcacgttg tcgaccgcgt
      721 tgtggtgctc ggtgatgccg agggtccagc agaccatcgc ccggtcggcc gtggcgtacg
      781 cgtgggcggc ctcgcggatc gcggcggccg ggacgcccgt ctcgcgctcc gcgtactcga
      841 gcgtgtacgg ctcgacgccc gcccgatagg cctcgaaccc ggtcgtcgcc cgggcgatga
      901 attccgagtt ggccaggccc gcgtggatga tctcgcgcgc catcgcgttg gccaaggcga
      961 tgtccgagcc gacgtcgagg cccagccaca ggtccgccca ctgcgcgctc gacgtccggc
     1021 gcgggtcgac cgcgatcagc cgggcacccc ggcgcacccc cttcaggagg tggtggaaga
     1081 agatcgggtg cgtctcccgg gcgttgctgc cccacaggag gaagcagtcc gtctcttcag
     1141 cttcctggta tgagctggtt ccgccaccag cgccgaacac ggtcgccaga ccggcgacag
     1201 agggggcgtg tcaggtgcgg ttgcagctgt cgatgttgtt gctgcccagg acggcacggg
     1261 agaacttctg ggccgcgaag ttgacctcgt tggtcgactt gctgcagctg aagactccgt
     1321 acgccccgga gccgtgcgcc gcgcccgcgg ctcggagccc ggcggccgcc cgttccagcg
     1381 cttcgtccca ggacgcggga cgcaggacac cggtctggcg gtcgccgtcg cgcacgagcg
     1441 gcgtggtgag gcggacgtac tccttcggca caggcgacct cgggtggaga gtccaacggc
     1501 ctcgagtgta atcgcctgag ccgctcagcc gcgcgcgacc acctcggccg tcggcgggtc
     1561 gtacagcgcc atcagccggt cggcgacccc cttgcgagcg ccgacgaggt ggaggaagtc
     1621 gtcgccgtcc atctcgaaca gcaggccgtc ggtggccgcg gtgacggtcg cggtgcgcgg
     1681 cgtctggccg atgaggccgc gctcgccgaa gacctcgtcg gtgcccagcg tccgcagatg
     1741 gcgcggttcg ccgcccgccg tgtccgcctg ggtgacgtcg aacgagccgg tgtcgatcac
     1801 gtagaagcgg tcagctgggt cgccctcgcg gacgatgacg tcgccggcgt ggacggccag
     1861 cggctcgagc cgccgcaacg cgccctccac ccgcgcgggc ggcagcccac cgaacaggga
     1921 caggcgggcg atgcgtcgca gggcgaggtc gtgctcgccg atggggacgt ctccggcgcc
     1981 cgcgatgagc gcgacggcga ggatggtcgc ggccgccacg ccgacgcccg cgaagcccat
     2041 gacgatgccg aacccgaaca ggctcacgag gatcggcacg atgagcgttc cgccggcttc
     2101 cgagagcgtc tgggaggtca tcatcaggcc gccaaaccgg ccgcggtact cgtcggggac
     2161 cacccgctga aagatcgtcg tgcgggtcac atcgaggatg aggttgccgg ccgagatcac
     2221 ggcgatggcc aggaaggcga cggccagctg attcgcgagg ccgaggatca cggtcgcgac
     2281 cgcgaacgcg gcggccccga cgagcagcac cccgccgagc cgcggccgga gcacgagcgc
     2341 ccctgacaac agcgcgccga tcgtgcctcc gatgccgatc gcggcgttga ggtacccggt
     2401 cgccgcgtcg cccccgtgga acacgtccac cgcgatgacc acgatcaaga tgctgatgcc
     2461 gccgaaggtg aaccaggtga aggcgtcgat gagcaccacg ccagcgaggg ccgacaggtt
     2521 gatcgatgcc gggacccggc ggccgaggaa gacgcgaccc tcgggctcgg gcgtttcggt
     2581 cgcggcgtcg agggcggccg cgctttcctc ggacggggcc ggcccggcgc tgctcgccgc
     2641 gcccgggctg ctccgcggca gggtccacag gatcacggcg atcagcgtga aggacacggc
     2701 gttgagcacg aatgcgagca cgagttcgcc ggtcgcgagc aacacaccgg cgatcccggg
     2761 cccgacgatc caggccatgt tgtcgagcgt cgcccaggcc gagttcgcgg gcccgaactc
     2821 gcgctcgtcg cggacgaggc tgggtatcag gg
//
LOCUS       DBNHXO010000485         2238 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_948393, whole genome shotgun sequence.
ACCESSION   DBNHXO010000485 DBNHXO010000000
VERSION     DBNHXO010000485.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_948393"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="AC162A_09825"
     CDS             <1..915
                     /locus_tag="AC162A_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HIR5011184.1"
                     /translation="QALVTRLEGAALAAEEAGFLGDNVLNSGQRVTVTIRPVQGAFML
                     GEETVLLKALEGKRGQPEQKPPYPTEKGLFGRPTVVNNVQTLAAVPWIVRQGSAAYAA
                     IGAEDSPGTVLVQLRGPAGGGIAEVPLGTPLRKLVDLAGGKGKQTVKAVLVGGPTGGL
                     LPPDQLDTPYTYGPLREAGAHMGSGSVVVADERACIVDLARLLTRFSADEACGKTIPC
                     RIGLRRLYEIGDRIATGRPKPTDAVLLGDLAHDVAASGLCDHERLATLAFTSGMRYFR
                     SEIDDHILRSSCPAGVCQPIAVAAGAAH"
     gene            925..>2238
                     /locus_tag="AC162A_09830"
     CDS             925..>2238
                     /locus_tag="AC162A_09830"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5011185.1"
                     /translation="MADLIIRPERPPERRVQSRPDRPAVLDTVTPQGDSHAERLLTTQ
                     APQRPQNTPAIRIEVDGRVVEGLEGQTILEVCRDNGIEIPTLCYEPKLPGFGACRMCV
                     VEVEGEDNPPISCSRQAEEGMVVATQTEEMRRLRRTNLELIFSDHNAYCLPPCQNKCP
                     SHIDIPGFLKANAEANWRESTRIFKRTIPFPSVLGRVCPAPCEEHCRRDEVDEAIAIR
                     DSHRYAGDQVIRAMLDEDLDPPLPFEQQATSGRRAAVIGSGPAGMAAAYYLLLAGHDV
                     TVFERDPSPGGMLRYGIPQYRLPKVEVLEAEYESVTRLGGKMECNKGLGRDFTLDDLQ
                     NQGFDAVVVAIGCYDTNKLGIPNEDADGVIDGLEYLRTATLGLPYPGHEGSRVVVIGG
                     GFTSMDCSRTSVRQGASEVTLVYRRDMKDMPASNEVHEAIEEGVTA"
BASE COUNT          352 a          836 c          758 g          292 t
ORIGIN      
        1 caggcgctgg tcacgcgcct cgagggcgcg gcgctcgcgg ccgaggaggc cggcttcctg
       61 ggcgacaacg tcctcaacag cggccagcgc gtgaccgtca cgatccgccc cgtccagggc
      121 gcgttcatgc tgggcgagga gaccgtcctg ctcaaggcgc tcgagggcaa gcgcggccaa
      181 ccggagcaga agccgcccta cccgaccgag aagggcctgt tcgggcggcc gacggtcgtc
      241 aacaacgtcc agacgctggc cgccgttccg tggatcgtgc gccagggcag cgccgcgtat
      301 gccgcgatcg gcgccgaaga cagcccgggg accgtcctcg tccagctgcg cggacccgcc
      361 ggtggcggca tcgccgaggt cccgctgggg actccgctgc gcaagctcgt cgacctcgca
      421 ggcggcaagg gcaagcagac ggtcaaggcc gtgctggtcg gcggccccac cggcggcctg
      481 ctgccacccg accagctcga cacgccctac acctacggac ccctccgcga ggccggcgcc
      541 cacatgggat ccgggtcggt cgtggtcgcc gacgaacggg cgtgcatcgt ggacctcgcg
      601 cggctcctga cccggttctc ggcggacgag gcgtgcggca agacgatccc ctgccggatc
      661 gggctgcggc gcctgtacga gatcggcgac cggatcgcca ccgggcgccc gaagcccacc
      721 gatgccgtcc tccttggcga tctcgcgcac gatgtcgcgg ccagcggcct gtgcgaccac
      781 gagcgcctgg cgaccctggc gttcacctcg gggatgcgat acttccgctc cgagatcgac
      841 gatcacatcc tgcgcagctc atgccccgcc ggcgtctgtc agccgatcgc ggtggcggcg
      901 ggcgcggccc actaggaccc gcgaatggct gacctgatca tccgtcccga acgcccgccc
      961 gagcgccgcg tccaatcgcg cccggaccgc ccggccgtcc tcgacacggt cactccgcag
     1021 ggcgattcgc acgccgagcg gctgctcacg acccaggcac cccagcggcc gcagaacacg
     1081 cccgcgatcc ggatcgaggt cgacgggcgg gtcgtcgagg gtctcgaggg ccagacgatc
     1141 ctcgaggtct gccgcgacaa cgggatcgag atcccgacgc tgtgctacga gcccaagctg
     1201 ccgggcttcg gcgcctgccg gatgtgcgtc gtcgaggtcg agggcgagga caacccgccg
     1261 atctcgtgct cacgccaggc cgaggagggc atggtcgtcg cgacccagac cgaggagatg
     1321 cgccggctac ggcggacgaa cctcgagctg atcttctccg accacaacgc ctactgcctg
     1381 cccccgtgcc agaacaagtg ccccagccac atcgacatcc ccggcttcct caaggccaac
     1441 gccgaggcca actggcgcga gtcgacccgg atcttcaagc gcacgatccc gttcccctcc
     1501 gtcctcggcc gcgtgtgccc ggccccgtgc gaggagcact gccggcggga cgaggtggac
     1561 gaggcgatcg cgatccgcga cagccatcgc tatgccggcg accaggtcat ccgggcgatg
     1621 ctcgacgagg acctcgaccc gccgctgccg ttcgagcagc aggcgaccag cggccgtcgt
     1681 gccgccgtga tcggctccgg cccagccggc atggctgcgg cctactacct gctgctggcc
     1741 ggccacgacg tgacggtgtt cgagcgcgac ccctcgcccg gcggcatgct ccgctacggg
     1801 atcccgcagt atcgcctgcc caaggtcgag gtcctcgagg ccgagtacga gtcggtcacc
     1861 cgcctcggcg gcaagatgga atgcaacaag ggcctggggc gcgacttcac gctcgacgac
     1921 ctccagaatc agggcttcga tgcggtcgtc gtcgcgatcg gctgctacga caccaacaag
     1981 ctgggcatcc ccaacgagga cgcggacggc gtcatcgacg gcctcgagta cctgcgcacg
     2041 gccacgctcg gtctgcccta cccgggccac gagggcagcc gggtcgtcgt catcggcggc
     2101 gggttcacct cgatggactg ctcgcggacc tcggtccgcc agggcgccag cgaggtcacg
     2161 ctcgtctacc gccgcgacat gaaggacatg ccggccagca acgaggtcca cgaggcgatc
     2221 gaggaaggcg tcaccgcc
//
LOCUS       DBNHXO010000486         1886 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1742568, whole genome shotgun sequence.
ACCESSION   DBNHXO010000486 DBNHXO010000000
VERSION     DBNHXO010000486.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1742568"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="AC162A_09835"
     CDS             complement(<1..826)
                     /locus_tag="AC162A_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR5011186.1"
                     /translation="MTALAPRVRTPAQPGPGPAPEALLRALDLSIGRRIRGLVAGEFR
                     AADLGGGTELAQVRPYEPGDDVRRIDWNATARMATPHVRVHVPERALTSWLLLDVSPS
                     MNFGTTDRRKTDVAEGVAIAIGHLSTQRGNRLGVLTFGGATDRQSRPLAGRAGLLMTL
                     AAVELEADGRESGFKSPDAAINFVAHGAPAGGLVVLVSDLRGPLDWLPQLGAVAQRHS
                     VLVVEIRDPREDELPDVGELNLIDAETGRTVRVDTSSSTLRTRFAEAAARDRDHVAR"
     gene            complement(823..1851)
                     /locus_tag="AC162A_09840"
     CDS             complement(823..1851)
                     /locus_tag="AC162A_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5011187.1"
                     /translation="MIEPVRDARPSLPAAPTGDPRTAAEAVLFEIRRVIVGQDAMLER
                     VLVALLSGGHLLLEGVPGLAKTLTIRTLADALDASFRRIQFTPDLVPADLVGTRIYRP
                     STGNFDTELGPVFCNFLLADEINRAPAKVQSALLEVMQERQVTIGRESHKVPSPFLVM
                     ATQNPIESEGTYPLPEAQVDRFMMKVVVGYPTAAEEATVVERSLRPAPTVRAILDADS
                     LAVLQAATPNIYVDPAITAYAVSIVAATRNPELVGQGALKGYIAFGASPRGSINLVHA
                     ARALALLRGRRYVVPADIADLAPDVLRHRIVPSFSALAEEVTPDMILSRIIPVVPAPR
                     LITEERTA"
BASE COUNT          255 a          614 c          771 g          246 t
ORIGIN      
        1 cgcgggcgac gtggtcgcga tcgcgggccg ccgcctcggc aaaccgggtc cgtagggtgc
       61 tcgatgaggt gtcgacgcgg acggtccgac ccgtctcggc gtcgatgagg ttgagctcgc
      121 ccacgtctgg cagctcgtcc tcgcggggat cgcggatctc gacgaccagc acgctgtggc
      181 gctgggcgac cgcaccgagc tggggcagcc agtcgagcgg cccgcggagg tcggacacga
      241 ggaccacgag gcccccggcc ggcgcgccgt gggcgacgaa gttgatcgcg gcgtcgggcg
      301 acttgaagcc ggactctcgg ccgtcggcct cgagctccac ggcggcgagc gtcatcagca
      361 ggccggcgcg gccggcgagc ggtcgcgact ggcggtcggt cgcgccaccg aaggtgagga
      421 cgccgagccg gttgccgcgc tgcgtcgaga ggtggccgat cgcgatcgcg acgccctcgg
      481 cgacgtcggt cttgcgccgg tccgtggtcc cgaagttcat cgagggcgag acgtcgagca
      541 ggagccacga cgtgagcgcg cgctccggga cgtggacccg cacgtgcggg gtcgccatcc
      601 gggcggtcgc gttccagtcg atgcgcctga catcgtcgcc cggctcgtac ggccggacct
      661 gggcgagctc cgtcccgccg ccgagatcgg cggcgcggaa ctcgccggcg acgaggccgc
      721 ggatgcggcg cccgatcgag aggtcgagcg cgcgcaggag cgcctcgggc gcgggaccgg
      781 gacccggctg ggccggcgtc cggactcgcg gcgcgagcgc ggtcatgccg tccgctcctc
      841 cgtgatcagg cgcggcgcgg ggacgaccgg gatgatccgc gacaggatca tgtccggcgt
      901 gacctcctcg gcgagggccg agaagctggg gacgatgcgg tggcgcagga cgtcgggcgc
      961 gaggtcggcg atgtcggccg ggacgacgta ccggcgaccg cgcagcaggg ccagcgcgcg
     1021 ggcggcgtgg acgaggttga tcgacccgcg ggggctggcg ccgaaggcga tgtagccctt
     1081 gagggcgccc tgcccgacga gctcgggatt gcgcgtggcg gcgacgatcg agaccgcgta
     1141 ggccgtgatc gccgggtcga cgtagatgtt gggggtcgcg gcctggagca cggcgaggga
     1201 gtcggcgtcg aggatcgcgc gcaccgtcgg cgccggccgg agggagcgct cgacgaccgt
     1261 ggcctcctcg gcggcggtcg gatagccgac gacgaccttc atcatgaacc gatcgacctg
     1321 tgcctcgggc agcgggtagg tgccctcgga ctcgatcggg ttctgggtcg ccatcacgag
     1381 gaacgggctc gggaccttgt ggctctcgcg gccgatcgtg acctggcgct cctgcatcac
     1441 ctcgagcagc gcggactgga ccttggccgg cgcgcggttg atctcgtcgg cgagcaggaa
     1501 gttgcagaac accgggccga gctcggtgtc gaagttgccg gtcgaggggc ggtagatccg
     1561 ggtaccgacg aggtccgcgg ggacgaggtc gggcgtgaac tggatccggc ggaacgacgc
     1621 gtcgagcgcg tccgcgaggg tccggatcgt gagcgtcttg gcgaggccgg gcacgccttc
     1681 gagcagcagg tggccgcccg agagcagggc cacgagcacg cgctcgagca tcgcatcctg
     1741 gccgacgatc acgcgccgga tctcgaacag gacggcctcg gccgccgttc ggggatcgcc
     1801 ggtcggagcg gcggggagcg acggtcgggc gtcgcgaacg ggttcgatca tcgggagcct
     1861 ccggtacccg cctgggcggg cgtggt
//
LOCUS       DBNHXO010000487         1945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2934282, whole genome shotgun sequence.
ACCESSION   DBNHXO010000487 DBNHXO010000000
VERSION     DBNHXO010000487.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2934282"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..>1945
                     /locus_tag="AC162A_09845"
     CDS             87..>1945
                     /locus_tag="AC162A_09845"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5011188.1"
                     /translation="MRTYAPAPTIAVLDRLLEEPSLARGVLHHEVRPAVDAEFGEWPA
                     WLDPRLRAGLARRGIERPYVHQAAAIEAVHGGEDIVVVTPTASGTSLCYMAPVLQALA
                     EDPSARALFLFPTKALGQDQVAELSELVREAELEVSSACYDGDTPAPIRSAIRKAGQV
                     VVTNPDMLHSAILPHHTKWFQLFEQLRIIVIDELHMYRGVFGSHVANVLRRLLRLCAH
                     YGSSPVIVCCSATIGNPSELAELLVGRPVRTIDRNGAPAGEKHLLLVDPPILDGSTGA
                     RGSALTLAERWALPFLRAGRQTVVFGRSRVAVEIMLSNLREALRMDLGPRSKLRGYRG
                     GYLPTERRSIERGLRDGEILGVVSTNALELGVDIGRLDVAVLAGYPGSIAATWQQMGR
                     AGRRGDVSVAILVASPAPVDQYVIHHPEFLLGGQPEEARLDPDNLHVLLAHLRAAVFE
                     LPFDPGDRFGPEPADGLLGFLGEGGQVRQADDGRWYWSSENFPASEISLRSAAPENVV
                     IIDTTPDRPRVLGEVDLFSAQVLVHEHAIYMHESIQYHVDRLEWGERKAYVHRIDADH
                     YTYANRAVTLKPLDVFASTPATGGSRVHGEVMVASLVTLYKKLKFITDENVGWG"
BASE COUNT          273 a          704 c          680 g          288 t
ORIGIN      
        1 gcgagctgtc acggcgtgca tgcagggtgg cgtagactgc gcgccaaacc gatcatgcgt
       61 tcgatatccg tgccggagcc gagctgatgc gcacctacgc cccagccccc accatcgccg
      121 tcctggatcg gctgctcgag gagccgtcgc tcgcccgcgg cgtgctccac cacgaggtcc
      181 ggccggcggt cgatgccgag ttcggcgagt ggccggcctg gctggacccg aggcttcgtg
      241 ccggcctggc caggcgcggg atcgagcggc cgtatgtcca ccaggccgcg gcaatcgaag
      301 cggtccatgg cggcgaagac atcgtcgtgg tcacgcccac ggcgtccggc acgtcgctgt
      361 gctacatggc gccggtcctc caggcgctgg cggaggaccc gtcggcgcga gccctgttcc
      421 tgttccccac caaagcgctg gggcaggacc aggtcgccga gctgtcggag ctcgtccggg
      481 aggcggagct cgaggtgtcg tccgcctgct acgacgggga cacgcccgcg ccgatccggt
      541 cggcgatccg caaggccggg caggtcgtgg tgaccaaccc cgacatgctc cactcggcga
      601 tcctccccca ccacaccaag tggttccagc tgttcgagca gctccggatc atcgtgatcg
      661 acgagctgca catgtaccgg ggcgtgttcg gcagccacgt cgcgaacgtc ctgcggcggc
      721 tgctccggct gtgcgcccac tacggcagca gccccgtcat cgtgtgctgc tcggccacga
      781 tcgggaaccc gtcggagctg gcagagctgc tggtcggccg ccccgtccgg accatcgacc
      841 gcaacggcgc gcccgccggg gagaagcacc tgctcctcgt cgatcccccg attctcgacg
      901 gctcgacggg cgcgaggggc tcggcgctga ccctggccga gcgatgggcg ctcccgttcc
      961 tccgcgcggg tcgccagacg gtcgtgttcg ggcgctcgcg ggtcgccgtc gagatcatgc
     1021 tctcgaacct gcgcgaggcg ctgcggatgg accttgggcc gcgctcgaag ctacggggct
     1081 atcgcggcgg atacctgccg accgagcgtc gctcgatcga gcgagggctg cgcgacggcg
     1141 agatcctcgg cgtcgtgagc accaacgcgc tcgagctcgg ggtcgacatc gggcgcctcg
     1201 acgtcgccgt cctggccggc tatccgggct cgatcgccgc cacctggcag cagatggggc
     1261 gggccggccg gcggggcgac gtcagcgtcg cgatcctcgt cgcgtcgccc gcccccgtgg
     1321 accagtacgt catccatcac cccgagttcc tcctgggcgg ccagcccgag gaggctcgcc
     1381 tcgacccgga caacctccac gtcctgctgg cccacctgcg ggcggccgtg ttcgagctcc
     1441 cgttcgaccc gggcgaccgg ttcggcccgg agcccgccga cgggctgctc ggcttcctcg
     1501 gcgagggcgg ccaggtccgc caggcggacg acgggcgctg gtactggagc tccgagaact
     1561 tccccgcctc ggagatctcg ctccggtcgg cggcgcccga gaacgtggtg atcatcgaca
     1621 cgaccccgga ccggccgcgg gtcctgggcg aggttgactt gttcagcgcc caggtactcg
     1681 tccacgagca cgcgatctac atgcacgagt cgatccagta ccacgtcgac cggctggagt
     1741 ggggcgagcg gaaggcgtac gtccaccgca tcgacgccga ccactacacg tacgcgaacc
     1801 gggcggtgac cctcaagccg ctcgacgtgt tcgcctcgac gccggcgacc ggcgggtcgc
     1861 gggtccacgg cgaggtcatg gtcgcgagcc tcgtgacgct gtacaagaag ctcaagttca
     1921 tcaccgacga gaacgtcggc tgggg
//
LOCUS       DBNHXO010000488         6665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_358862, whole genome shotgun sequence.
ACCESSION   DBNHXO010000488 DBNHXO010000000
VERSION     DBNHXO010000488.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_358862"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="AC162A_09850"
     CDS             complement(<1..587)
                     /locus_tag="AC162A_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5011189.1"
                     /translation="MIDVDALLAMKGIEKGFPGVKALAGVDFDVREGEIHAIVGENGA
                     GKSTLIKVMTGVERPDAGTITLGGTDIHVRSPQHAQSLGISTVYQEVNLCPNLSVGEN
                     ILIGRAPRRFGMIDSGALHRRAAEILRSLDVEVDVTQPLGSFSVAIQQMAAIARALEI
                     SSARILILDEPTSSLDAHETEQLFGVMRKLKRQGLA"
     gene            complement(709..1704)
                     /locus_tag="AC162A_09855"
     CDS             complement(709..1704)
                     /locus_tag="AC162A_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5011190.1"
                     /translation="MTRSRTLKPIAAALAVLALAAACSSGGGSSAAPASAAAKTFKDL
                     TVGFIQTGSEGGWRAANTASFKDEAEKLGLTLKFYDAQNKMENQLKAFRDFIADDSVD
                     VIVLAALEAAGWDDVLKEAKAKGKTVVLEDRRIDAPEDLYATYIGSDFTEEGRKAATE
                     MCTLLEGSAKKNVVELVGNVGSSAAIDRGKGFREKMGDCGITITESQTADWDSTKGKA
                     VTEAFLKKSKDIQGVFAQNDEMGLGAVQALTEAGLKPSEDVKIITVDATKGAFQAMID
                     GTINTVVECNPLLAKQVYEAALKAVNGETLPKWVPSIESVFHAADAAEILPTRQY"
     gene            2052..3068
                     /locus_tag="AC162A_09860"
     CDS             2052..3068
                     /locus_tag="AC162A_09860"
                     /inference="COORDINATES: protein motif:HMM:NF024799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR5011191.1"
                     /translation="MAQAAGVSTQTVSRVLNNRPDVAPETFERVQRIISDTGYAPNML
                     ARSLTQGRSHILGVVAFGLDYFGPSRILTGIEQRAADLGYSISLNLIHRPETADVDEV
                     LNGLIGRQVDGIIWAIPEVAENRAWSHERSPDLPVPVMLIGSPAGNSSLPSIGIDNRA
                     IGQLATEHLIAGGVRRVAVVTGPRGWWEAEERIDGWRATLDAHGIGEQDRYVVDGDWG
                     ASSGERALDQLMREFPDVDAIFASNDQMALGVLHGAHRMGKRVPEDLSIVGVDDIPEA
                     SHFWPSLTTVHQPLADAGALAVEAIDRSIGDDRDRRERDVQPASVTLLQPTLIVRDSS
                     RRVD"
     gene            complement(3128..4363)
                     /locus_tag="AC162A_09865"
     CDS             complement(3128..4363)
                     /locus_tag="AC162A_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5011192.1"
                     /translation="MKVTTSQPAATGSLRLAWILLIVVALVIVAAGIAVVGGRLLTPS
                     PALPRGGAAVLAFTSLQGGAIGQPVGDLYTVRADGTDLRRLTNAANTSGFDQAPLWSR
                     DGSRIVFHNYQGAKDSVVVIDAGGGNRTTLWTSVTFRDAYCAEHDDFSWSPDGQTVAF
                     AAHETCPGEPDLFVVPADGSAPAGRLLPPGTNGVFPRFSPDGSRIAFLGREGGGATGL
                     YVAEVGSAGAGAGGVTARRIGPDVAGSAGEQWFPPQWSPDGSELALATGFEPSEIVIV
                     KADGSGQRVLATDVAVNGSWSPDGQRIAFHRIVDESERWQGRPCTMRVWVINADGSGE
                     RRLDPLVDGCAFQPIWSPDGTRLLSLMIVDEAFHVGVLDVDGDDSPVTFQESYGASWQ
                     PVAAPLPPAPSFASGSPTP"
     gene            complement(4516..5070)
                     /locus_tag="AC162A_09870"
     CDS             complement(4516..5070)
                     /locus_tag="AC162A_09870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5011193.1"
                     /translation="MQQDLVDRARKGDHDAFAVLAGATIGRLDAAARLILRDPDRAQD
                     AVQETLVRCWRDLPTLRDSERFDAWLHRLFLNACRDELRTARRRSIEVVLPEIFPLSV
                     PDTQTASADQDEIERGVSRLDPEHRTVIVMHYYLGLPLPEAAAAMGIPVGTAKSRLHR
                     ATQALRAALDSDARTHPGLTEGRS"
     gene            5319..6407
                     /locus_tag="AC162A_09875"
     CDS             5319..6407
                     /locus_tag="AC162A_09875"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HIR5011194.1"
                     /translation="MRHRRTTGLITAGLVLIATLVTAAPAAAHGGGHGKVVVLLDGLS
                     SPKGLEISGRSIFVAQGWAGAPGPVLEYRQIGRWHKSVRPVTDPLSLVDIVGTRDGAG
                     WAIGTDGVLYRQATPGGPIDPVLDILAYQAIDIDPTDQDDPPNPGESNPNGLAALRSK
                     VLIADAAGNDLLRVSPNGKAVTVARWTNEEIVTDDPALPPTMLAEAVPTTVAIGPDGW
                     AYVGQLVGFPGTPGSAHIWRVNPWAKDAVCDVNHPNRDCKVWKSGFTSIFDIAFAPHS
                     WTLYVYEIAEDGWLAFEEGFATGEFPPAVLLEVKGHRIRELARGQLSQPGGVEVGRDG
                     QVYVTDGMFTDGRLIRIRGRGHGYGHHH"
     gene            complement(6437..>6665)
                     /locus_tag="AC162A_09880"
     CDS             complement(6437..>6665)
                     /locus_tag="AC162A_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate cyclase"
                     /protein_id="HIR5011195.1"
                     /translation="VDPKKFEHEVVAERWKLPDGSDLVELSIKVEPGEATQAADGFTT
                     FLEGLGLDVGGDQQTKTRGALTFFTTGKGFD"
BASE COUNT          993 a         2298 c         2366 g         1008 t
ORIGIN      
        1 gcgaggccct ggcgcttgag cttgcgcatc acgccgaaca gctgctcggt ctcgtgggcg
       61 tccaggctcg aggtcggctc gtcgaggatg aggatccggg cggacgagat ctccagcgcc
      121 cgcgcgatcg ccgccatctg ctggatcgcg accgagaacg agcccagcgg ctgggtcacg
      181 tcgacctcga cgtcgaggct gcgcaggatc tcggcggcgc gccggtggag cgcaccggaa
      241 tcgatcatgc cgaagcgccg cggcgcccgg ccgatgagga tgttctcgcc gaccgagagg
      301 ttggggcaga ggttgacttc ctggtacacg gtgctgatgc ccagggactg ggcatgctgc
      361 ggggatcgga cgtggatgtc ggtgccgccc agggtgatcg tgcccgcgtc gggccgctcg
      421 accccggtca tgaccttgat caacgtcgac ttgccggcgc cgttctcgcc gacgatcgcg
      481 tggatctcgc cttcgcggac gtcgaagtcc acgcccgcga gcgccttgac gccgggaaag
      541 cccttctcga tgcccttcat cgccagcagc gcgtccacgt cgatcacgac ctacctgcgt
      601 gtccctggag ggtcctgggt gaaacatcga ggggcgaccc gaaggtcgcc cctcgatggg
      661 ctgcgcggcc gcacccgccg tggaggccgg cggggcggat cgggttgctc agtattggcg
      721 ggtcggcagg atctcggctg cgtcagccgc gtggaagacg ctctcgatcg aggggaccca
      781 cttggggagc gtctcgccgt tgacggcctt gagcgcggcc tcgtagacct gcttggccag
      841 cagcgggttg cactcgacga cggtgttgat ggtgccgtcg atcatcgcct ggaaggcgcc
      901 cttggtggcg tcaacggtga tgatcttgac gtcctcggac ggcttgaggc cggcctcggt
      961 gagtgcctgc acggcaccca ggcccatctc gtcgttctgg gcgaagacgc cctggatgtc
     1021 cttgctcttc ttgaggaacg cctcggtgac ggccttgccc ttggtggagt cccagtcggc
     1081 cgtctggctc tcggtgatcg tgatgccgca gtcgcccatc ttctcgcgga agcccttgcc
     1141 gcggtcgatg gccgcggagg atccgacgtt gccgacgagc tcgaccacgt tcttcttggc
     1201 gctgccctcg agcagggtgc acatctcggt cgccgccttg cggccttcct cggtgaagtc
     1261 cgagccgatg tacgtcgcgt acaggtcttc cggcgcatcg atgcggcggt cctcgagcac
     1321 gacggtcttg cccttggcct tggcctcctt gaggacgtcg tcccagccgg cggcttcgag
     1381 cgcggcgagc acgatcacgt cgacggagtc gtcggcgatg aagtcgcgga acgccttgag
     1441 ctggttctcc atcttgttct gcgcgtcgta gaacttgagg gtgaggccga gcttctccgc
     1501 ctcgtccttg aacgacgccg tgttggcggc acgccagccg ccttcggatc cggtctggat
     1561 gaacccgacc gtcaggtcct tgaaggtctt ggccgcggcg gacgccgggg cggcggagct
     1621 gccgcctccg gagctgcagg cagcagcaag ggcgaggacg gcgagtgccg cggcgatcgg
     1681 cttgagagtc ctggaacgtg tcactggtag acctccgggt ggtgcagcga tttcgcggat
     1741 ggggcgcgcg gggatcgtca ggctgggagg cgcgagggtc aggtcgtgcg tggcgccatg
     1801 gacacctcct ccggccccgc cgcccaggcc tggtgcccgg cgctgttgcc gccgggctgt
     1861 gcgtgcctcc cgggagacgc cgggagggct cgggcttgcg ggccaaccga actactgtta
     1921 ccgttcccgt taccgctaac gggaccgata acggtggcac ggtaacatcg cgctggcgga
     1981 cccgtcaaga cccgcgaatc gatcacccgc gaggatgaat ggcaactcgg cgcaggatga
     2041 cgatcaagga agtggcgcag gctgctgggg tttccaccca gaccgtctcg cgcgtcctga
     2101 acaaccggcc ggacgtcgcg cccgagacgt tcgagcgggt ccagcggatc atcagcgaca
     2161 cgggctacgc gcccaacatg ctcgcgcgga gcctcaccca gggccggagc cacatcctcg
     2221 gggtggtggc attcgggctc gactacttcg ggccgtcgcg catcctgacc ggcatcgagc
     2281 agcgggccgc cgacctcggc tactcgatct cgctgaacct cattcaccgg cccgagacgg
     2341 ccgacgtcga cgaggtgctc aacgggctca tcgggcgcca ggtggacggg atcatctggg
     2401 ccatccccga ggtcgccgag aaccgcgcct ggtcgcacga gcgcagcccg gacctcccgg
     2461 tccccgtgat gctgatcggg agccctgccg gcaactcgtc actgccctcg atcgggatcg
     2521 acaacagggc aatcgggcag ctggcgacgg agcacctgat cgccggtggc gtgcgtcgag
     2581 tcgcggtcgt caccgggccg cggggctggt gggaggccga ggagcgcatc gacggctggc
     2641 gcgcgaccct ggacgcccat ggcatcgggg agcaggaccg ctacgtcgtc gacggtgact
     2701 ggggtgccag cagcggcgag cgcgcgctcg accagctgat gcgggagttc cccgacgtcg
     2761 acgcgatctt cgcgagcaac gaccagatgg ccctcggagt cctgcatggc gcccaccgga
     2821 tgggcaagcg ggtgcccgaa gacctctcga tcgtcggcgt cgacgacatc cccgaggcgt
     2881 cccacttctg gccgtcgctg accacggtgc accagcccct ggccgacgct ggcgcactcg
     2941 cggtcgaggc gatcgaccgc agcatcggcg acgaccgcga ccggcgcgag cgggacgtcc
     3001 agcccgcgtc ggtcaccctg ctgcagccga ccctgatcgt ccgcgacagc agccgccggg
     3061 tggactgacc gcccgaatcc gatgaccccg gcgggccgcc ggggtcatcg acaaagccgg
     3121 ccgatgccta cggcgtcggg gagccactcg cgaaggacgg cgccggcggc agcggcgcgg
     3181 ccaccggttg ccaggacgcg ccgtaggact cctggaaggt gaccggcgaa tcgtcgccat
     3241 ccacgtcgag gacaccgaca tggaacgctt cgtccacgat catgaggctc aggagtcggg
     3301 tcccgtcggg ggaccagatg ggctggaagg cgcagccgtc gaccaagggg tcgagccgtc
     3361 gctcgcctga gccgtccgcg ttgatgaccc acacccgcat cgtgcacggc cgaccctgcc
     3421 agcgctccga ttcgtcgacg atgcggtgga acgcgatgcg ctgaccgtcc ggcgaccagc
     3481 tcccgttaac cgccacgtcg gttgcgagga cgcgctgtcc cgatccgtcg gccttcacga
     3541 tgacgatctc gcttggctcg aacccggtcg cgagcgcgag ctccgaacca tcgggggacc
     3601 actgcggcgg gaaccactgt tcccccgcgc tgccggcgac atcgggtccg atgcgacggg
     3661 ccgtgacccc gcccgcaccg gcgccagccg atcccacctc ggccacgtag aggccggtgg
     3721 cgccgccgcc ttcacggcca aggaaggcga tccggctgcc gtcgggcgag aacctcggga
     3781 agacgccgtt cgtgccgggc ggcaggagcc tgcccgccgg cgccgacccg tccgccggga
     3841 cgacgaacag gtcgggctcg ccggggcacg tctcgtgggc ggcgaacgcc accgtctgcc
     3901 cgtccggcga ccacgagaag tcgtcgtgct cggcgcagta cgcatcccgg aatgtgacac
     3961 tcgtccacag ggtcgtccgg ttcccgccgc cggcatcgat caccacgacc gagtccttcg
     4021 ccccctggta gttgtggaag acgatgcgcg agccatcgcg cgaccacagt ggtgcctggt
     4081 cgaagccgga agtattggcg gcattcgtca accggcgcag gtccgtcccg tcggccctga
     4141 cggtgtagag gtcgcccacc ggctgtccga ttgcgccacc ctgcagggac gtgaacgcga
     4201 ggacggctgc gccgccgcga gggagggccg gggacggcgt gaggagccgg ccgccgacga
     4261 cggcgatccc cgccgcaacg atcaccaggg cgacgacgat gagcaggatc cacgcgagcc
     4321 gcagcgaacc ggtcgccgcg ggttggcttg tcgtaacctt catgggaagc caccttccga
     4381 ggcttgccca ggctggccgc tgcctcgtgc gccgcgtccg ctccaggacc tgtgcgaggt
     4441 aggcgggagc aggcggggcg aacgcgtcct caggccacgc ctcgagccgc cgggtgacat
     4501 cgggccgttg ctgcatcagg aacgaccctc cgtcagtccg gggtgcgtgc gtgcgtccga
     4561 gtcgagcgcg gcgcgcagcg cctgggtcgc ccggtggagc cgcgacttgg cggtgccaac
     4621 cgggatgccc atggccgcgg ccgcctcggg aagcggcagg cccaggtagt agtgcatgac
     4681 gatgaccgtt cggtgctcgg ggtcgaggcg gctcacgcca cgctcgatct cgtcctggtc
     4741 ggcggatgcc gtctgggtat cgggcaccga gaggggaaag atttcgggca gcacgacctc
     4801 gatgcttcgt cgccgggctg tccggagctc atcgcggcag gcgttgagga agagccggtg
     4861 gagccaggcg tcgaagcgct cggaatcgcg cagtgtcggg aggtcgcgcc agcagcggac
     4921 gagcgtctcc tggacggcgt cctgggcgcg atcgggatcc cgcaggatga gccgcgctgc
     4981 ggcatccagc cgaccgatcg tggcgcctgc cagcacggcg aaggcgtcat gatcgccctt
     5041 gcgcgctcgg tccacgaggt cctgctgcac gttcgcctcg acggcccgcg cggccgactt
     5101 ggtcgcgcat gaagcgtttg gcgttaacgg gcggcgaatc gttcccaaat cgcgtcggga
     5161 agcgtgccag ccaacgtgcg ctgcatgggg acggtgcgtc cgcgcaccac aagcctagtc
     5221 caccccccgg gaccatcgct gccacgcgtt gacccggtcg agtcacgggc ggagactcgt
     5281 cgcttaccgc tgtcggggga cacgggagga gagacgcaat gcgccatcgg cgaaccaccg
     5341 gcctgatcac cgctgggctg gtcctgatcg cgaccctggt caccgcggca cccgccgccg
     5401 cacacggcgg gggacacggc aaggtggtcg tcctgctcga tggcctgtcg tcgcccaagg
     5461 gcctcgagat cagtggcagg tcgatcttcg tggcgcaggg ctgggccggc gcgcccggac
     5521 ccgtgctcga ataccgccag atcggccggt ggcacaagtc cgtgcgccca gtcaccgacc
     5581 cgctgagcct ggtcgacatc gtcgggaccc gcgacggcgc gggctgggcg atcggcaccg
     5641 acggcgtgct ctaccgccag gccacgccgg gcggtccgat cgaccccgtg ctcgacatcc
     5701 ttgcctacca ggccatcgac atcgacccta cggaccagga cgacccgccg aaccccggcg
     5761 agagcaaccc caacggcctc gcggccctgc gctcgaaggt gctcatcgcc gacgcggccg
     5821 gcaacgacct gctgcgggtg agcccgaacg gcaaggcagt caccgtggcg cgctggacca
     5881 acgaggagat cgtcaccgac gaccccgccc tcccgccgac gatgctggcc gaggccgtgc
     5941 cgacgaccgt ggccatcggc ccggacggct gggcctacgt gggccagctg gtcgggttcc
     6001 cgggaacccc gggctcggcg cacatctggc gcgtgaaccc ctgggccaag gacgcggtct
     6061 gtgacgtcaa ccacccgaat cgcgactgca aggtctggaa gtcggggttc acctcgatct
     6121 tcgatatcgc cttcgccccc cacagctgga cgctctacgt gtacgagatc gccgaggacg
     6181 gctggctcgc gttcgaggag ggcttcgcca cgggcgagtt cccgccggcg gtcctgctcg
     6241 aggtcaaggg ccaccggatc cgcgagctcg cccgcggaca gctgtcacag ccgggcggcg
     6301 tcgaggtcgg ccgcgacggc caggtctatg tcacggacgg catgttcacg gacgggcggc
     6361 tgatccggat ccggggccgc ggccacggtt acggccacca ccactgagcc ggggacgggt
     6421 cgccgtccca aggttctcag tcgaagccct tgcccgtggt gaagaaggtc agggcccccc
     6481 gcgtcttggt ctgctgatcg ccgccgacgt cgaggcccag gccctcgagg aaggtcgtga
     6541 agccgtcggc ggcctgggtc gcctcgcccg gctcgacctt gatcgagagc tcgacgaggt
     6601 ccgacccgtc gggtagcttc cagcgctcgg cgacgacctc gtgctcgaac ttcttggggt
     6661 cgacc
//
LOCUS       DBNHXO010000489         3185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1152240, whole genome shotgun sequence.
ACCESSION   DBNHXO010000489 DBNHXO010000000
VERSION     DBNHXO010000489.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1152240"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="AC162A_09885"
     CDS             <1..532
                     /locus_tag="AC162A_09885"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR5011196.1"
                     /translation="PRILERIPEIVIMGGAHDHGNATPSAEFNIWLDPEAARIVVNCG
                     RPIRMIPLDATHRALVSGEDCAALRALGTPAGEAAARFVEKRIEGYDATQPMHRAGAA
                     PVHDALAVCAVIDPSVVTTHFIPVDVETSGQLSDGRTVCDFRFRGGKPANVSFAMDAD
                     EPLFVRMLKDILGRSA"
     gene            complement(558..1430)
                     /locus_tag="AC162A_09890"
     CDS             complement(558..1430)
                     /locus_tag="AC162A_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HIR5011197.1"
                     /translation="MTDDAAALLTLHEAAERLGVHYMTVYRHVRLGMLPAHKVGGTWR
                     IDPADLVAARSRGADRTGAITPVGPALQGPRRRRAPWSERLSERMLAGDAAGSWQLVE
                     AAMASGVEPVDVYVEILAPALHGIGSGWQAGTIPIEREHLASSVAAAIVGRMGPRFRR
                     RGRHLGSVIIAMPVGERHGLGAAMLADIVADAGYEVLNVGPDTPPASLASAIRSRDDL
                     RAVVVSVVDAARQPAAARLIAAARAANRQVPIIAGGFAIPDLRTASAIGADAWAADPR
                     RLPALIAERAGRRA"
     gene            complement(1486..2277)
                     /gene="heR"
                     /locus_tag="AC162A_09895"
     CDS             complement(1486..2277)
                     /gene="heR"
                     /locus_tag="AC162A_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HIR5011198.1"
                     /translation="MHSSQAIRATAAHPVPVTTAVSLRRWNFTLSGLHAIQAVGILAL
                     ATAFALPVTASFLAGPPGTPPAPPTTLFDIPVAWGVALFLLLSAGFHGFIALPRTFER
                     YLAGLASERNFFRWTEYSLSSTVMIVLIAMITGISDVAALIGIAAANAAMILFGAVQE
                     RYESPGGSLWPFWLGSIVGIAPWIAIGVYLWSPGSVAEPPAFVYVIFVSLFAFFNVFA
                     LNMWLQYRQVGRWRSYLVGEKAYMVLSLVAKSALAWQVFAGTLAG"
     gene            complement(2361..>3185)
                     /locus_tag="AC162A_09900"
     CDS             complement(2361..>3185)
                     /locus_tag="AC162A_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HIR5011199.1"
                     /translation="TFSTLLTAATAAGLVETLQGAGPFTVFAPTDDAFAALPAGTLDK
                     LLADPEALKQVLLYHVVSGKVTSDQVVELTSADTVEGSPIAIAVKDGKVYLNDSAQVT
                     TADVEASNGVIHVIDQVLLPPATGASPAPSASAAAMDDIVATAKAAGTFNTLLQAATA
                     AGLVETLQGPGPFTVFAPTDEAFAALPAGTLDKLLADPEALKKVLLYHVVSGEVTADQ
                     VVGLTTADSVEGDPIAIAVKDGKVYLNDAAQVVATDIKASNGIIHVIDQVILPPSS"
BASE COUNT          512 a         1076 c         1160 g          437 t
ORIGIN      
        1 gccgcggatc ctggagcgga tcccggagat cgtgatcatg ggcggcgccc acgaccacgg
       61 gaacgcgacg ccgtcggcgg agttcaacat ctggctcgac cctgaagcgg cgcggatcgt
      121 cgtcaactgc ggccggccga tccggatgat tcccctggac gccacccacc gagctctcgt
      181 gtccggcgag gactgcgccg cgctccgcgc cctgggcacg ccggccggcg aggcagcggc
      241 gcgcttcgtc gagaagcgga tcgagggcta cgacgcgacc cagccgatgc atcgcgctgg
      301 cgccgcgccg gtccacgacg cactggcggt gtgcgcggtc atcgacccct cggtcgtcac
      361 gacgcacttc atcccggtgg acgtggagac gagcgggcag ctgtccgacg ggcggacggt
      421 ctgcgacttc cgcttccgcg gtggcaagcc ggcgaacgtg tcgttcgcga tggatgccga
      481 cgagccgctg ttcgtccgga tgctcaagga catcctgggc cggagcgcct gaaggcgtcg
      541 ccgcgcatga cgagcggtca ggctcgacgg ccggctcgct cggcgatcag cgccggcagc
      601 cggcgggggt ccgcggccca agcgtcggca ccgatcgccg acgccgtccg cagatccggg
      661 atcgcgaacc cgccggcgat gatcggcacc tgccggttgg cggcgcgcgc tgctgcgatg
      721 aggcgagccg cggccggctg gcgggccgca tccacgacgc tgaccacgac cgcacggagg
      781 tcgtcgcggg accggatcgc cgaggcaagc gacgcgggcg gcgtgtccgg gccgacgttc
      841 aggacctcat agccggcgtc ggcgacgatg tccgccagca tcgccgcacc cagcccgtgc
      901 cgctcgccga ctggcatcgc gatgatcacg ctcccgaggt ggcggccgcg acgacggaat
      961 cggggaccca tccggccgac gatcgcggcg gcgacgctgc tcgcgaggtg ctcccgctcg
     1021 atcgggatcg tgcccgcctg ccatccgctc ccgatcccgt gcagggccgg tgcgagaatc
     1081 tcgacgtaga cgtcgacagg ctcaacgccc gacgccatcg ccgcctcgac gagctgccag
     1141 ctcccggcgg cgtccccggc gagcatccgc tcgctgagtc gttccgacca cggagcgcgg
     1201 cgtcgtctgg gcccctggag cgccggcccg accggggtga tcgccccggt ccggtcggct
     1261 ccacgcgacc tcgcggcgac caggtcggca ggatcgatcc gccaggtgcc gcctaccttg
     1321 tgcgccggca gcatccccag ccgaacgtgg cgatagacgg tcatgtagtg cacgccaagc
     1381 cgctcggcgg cctcgtgcag cgtcagcagg gctgcggcgt cgtcggtcat tgctcccacg
     1441 cgggccggtc gccccacggc cgaacgcggc ccaggaggtg gtccatcagc cggccagcgt
     1501 accggcgaag acctgccatg cgagcgctga cttcgcgaca aggctcagga ccatgtacgc
     1561 cttctcgcca acgaggtacg agcgccaccg gccgacctgc cggtactgga gccacatgtt
     1621 gagcgcaaac acgttgaaga acgcgaacag cgacacgaag atcacgtaga caaacgccgg
     1681 cggctccgcc acgctgccgg gcgaccacag atacacgccg atggcgatcc atggcgcgat
     1741 gccgacgatg cttcccagcc agaacggcca tagcgaccca cccggcgatt cgtagcgctc
     1801 ctgaacggcg ccgaacagga tcatggcggc gttggcggcg gcgatcccga tcaacgccgc
     1861 gacatcgctg atgcccgtga tcatcgcgat cagcacgatc atcaccgtcg acgacagcga
     1921 gtactccgtc cagcggaaga agttccgttc gctcgcgagc ccggcgagat accgctcgaa
     1981 cgtgcggggg agcgcgatga acccgtgaaa gcccgctgag aggagcagga acagggcgac
     2041 gccccaggcg acagggatgt cgaatagcgt ggttggcggc gcgggtggcg tgcctggcgg
     2101 gccagcgagg aagctcgcgg tcaccggcag tgcgaacgcc gttgcgaggg ccaggatgcc
     2161 aacagcctgg atcgcgtgga gcccactgag ggtgaagttc cagcgacgga gcgagacggc
     2221 tgtggtgacg ggcactgggt gtgccgcggt ggctcggatg gcctgggagc tgtgcatggg
     2281 aagcctcgtc gaacgcagaa cgcggggaac ggcggccgga gaccggcgcc gtcccccgct
     2341 gggaggagga ggaacgaccg tcagctggac ggcggcagga tgacctggtc gatgacgtgg
     2401 atgatcccgt tcgatgcctt gatgtcagtc gcaacgacct gggcggcatc gttgaggtag
     2461 accttgccgt ccttgacggc gatggcgatg ggatcgcctt cgaccgagtc ggccgtcgtc
     2521 agcccgacga cctggtcggc ggtcacctcg ccggacacga cgtggtagag caggaccttc
     2581 ttgagggcct ccgggtcggc aagcagcttg tcaagcgttc cggccggcag cgccgcgaag
     2641 gcctcgtctg tcggggcgaa gacggtgaac gggccgggac cctgaagggt ctcgacgagg
     2701 cccgcagccg tggcggcctg caggagggtg ttgaaggtcc cggcggcctt cgcggtggca
     2761 acgatgtcat ccatcgcggc ggcgctggca gacggcgcag ggcttgcccc cgtggccggc
     2821 ggcagcagca cctggtcgat gacatggatg acgccgttcg acgcttcgac gtcggctgtg
     2881 gtgacctggg ccgagtcgtt gaggtagacc ttgccgtcct tgacggcgat ggcgatcggc
     2941 gagccctcaa ccgtgtcggc cgacgtgagc tccaccacct ggtcggacgt gaccttgccg
     3001 gacacgacgt gatacaggag gacctgcttc agcgcctccg gatcggccag cagcttgtcc
     3061 agcgtgccgg cggggagggc ggcgaacgcg tcgtccgtcg gcgcaaagac ggtgaacggg
     3121 ccggcgccct ggagggtctc gacgagaccc gcggcggtgg cagccgtcag cagggtgctg
     3181 aaggt
//
LOCUS       DBNHXO010000490         3733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3929817, whole genome shotgun sequence.
ACCESSION   DBNHXO010000490 DBNHXO010000000
VERSION     DBNHXO010000490.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3929817"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="AC162A_09905"
     CDS             complement(<1..893)
                     /locus_tag="AC162A_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR5011200.1"
                     /translation="MTAFTVAAVQAAYVLMDRDATIDRVAALTGEAAANGAKLVVFPE
                     AFVPGDPIWKDAGIIWDGDEAWYSMLVDQAIVVPSEATERLGAIAREHAVTLVVGIDE
                     REPNGATIYCSMLYFGSDGSLLGKHRKLMPTGSERTVWGMGDGSLLTVVDTEIGRVGG
                     LICWENYMPLARYYMYSQGIDVWVAPTLATGDAWVATMRHIAREGRLYVIGVNPCVRV
                     DQIPDDFPARDKVWKLEDDPDERDWVSPGNSVIIGPTGKMLAGPSRHEETILYADIDP
                     SEVRAARRYFDPTGHYNRPDV"
     gene            complement(945..1304)
                     /locus_tag="AC162A_09910"
     CDS             complement(945..1304)
                     /locus_tag="AC162A_09910"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR5011201.1"
                     /translation="MVAGPRRGDIHLVQFEDVGGHVLRGPHPAVVVSSDRLNRADGTV
                     LVCSMTTKRRVVAGGWLPPYLVPVTMRDTGLDRDGFVKCDQVFTRPATWLGARLGRLG
                     PEAMTRVDASLRFVLAL"
     gene            complement(1298..1630)
                     /locus_tag="AC162A_09915"
     CDS             complement(1298..1630)
                     /locus_tag="AC162A_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011202.1"
                     /translation="MQYDQQSDHGERPFPTFREALEPYLTPTESGPYAKLSISVPERL
                     LATVKEVADETGTSVSGVVAASLRRLVDEVEQARLDAALEADREENLEWARATAPMDA
                     KLLADVEW"
     gene            1741..3411
                     /locus_tag="AC162A_09920"
     CDS             1741..3411
                     /locus_tag="AC162A_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR5011203.1"
                     /translation="MPEPGAGADLLAMGREAFLREQWADAYARLTEADQGQSLGLDDL
                     ELAALAAYLVGLDEASVDLQTRAYAEALRGGDVDRAARRAFWLGFGLFYRGERVRGGG
                     WLARAARLAHEAGPESLVHGYLLVPPALVDHEAGDSEAASVAFREAARVAAKHGDADL
                     ETIARLGLGDALIGLGQVSHGMALLDEAMVSVTAGEVSPIVMGIVYCAVIETCQRIFD
                     LGRAHEWTAALAGWCDAHPDLVPYRGACLVYRADLMTLHGAWPEALAEARRASDASQR
                     PSVDPVSGEAAYRRAELHRLRGEATEAEEAYRHANRLGRTPQPGLALLRLDQGRVAVA
                     ASAIRRAVDGSTDVAARPRLLDAFVEISLAAGDVAAAGAAADELATLAGELDAPVLVA
                     LAARAEGAVLLAEGEVKSGLDELQRALAAWQAVEAPYEAARVRVLVAQACYRLGDDDT
                     ADLELDAADRAFRDLGAAPARRRLEALRDGRASGGRANAGGLTPRELEVLRQLASGKT
                     NRAIATDLVISEKTVARHVANIFTKLDLSSRSAATAYAYEHDLVAPAT"
     gene            3548..>3733
                     /locus_tag="AC162A_09925"
     CDS             3548..>3733
                     /locus_tag="AC162A_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5011204.1"
                     /translation="MQDGERFDAIVIGGGQAGLAAGYHLRRHGLRFVILDAGAHVGDA
                     WRSRWDSLRLFTPVRYDS"
BASE COUNT          512 a         1344 c         1330 g          547 t
ORIGIN      
        1 aacacgtccg ggcggttgta gtggccggtg gggtcgaagt agcgacgcgc cgcacggacc
       61 tcggacgggt cgatgtccgc gtacaggatc gtctcctcgt ggcgcgacgg tccggcgagc
      121 atcttgcccg tcgggccgat gattaccgag ttgccgggcg agacccagtc gcgctcatcc
      181 gggtcatcct cgagcttcca gaccttgtcc cgggctggga agtcgtcggg gatctggtcg
      241 acgcggacgc acgggttgac gccgatgacg tagaggcgcc cctcgcgggc gatgtggcgc
      301 atggtcgcca cccacgcgtc gccggtcgcg agcgtcggcg cgacccagac gtcgatcccc
      361 tggctgtaca tgtagtaccg cgccaggggc atgtagttct cccaacagat cagcccgccc
      421 acgcggccga tctccgtgtc gacgaccgtc agcaggctgc cgtcgcccat gccccagacc
      481 gtgcgctccg atcccgtggg catgagcttg cggtgcttgc cgagcaggct cccgtccgag
      541 ccgaagtaca gcatcgagca gtagatcgtg gcgccgttgg gctcgcgctc gtcgatgccg
      601 accacgagcg tcacggcgtg ttcccgggcg atcgcgccca gccgctcggt cgcctcgctg
      661 ggcacgacga tcgcctggtc gacgagcatc gagtaccagg cctcatcccc gtcccagatg
      721 atgcccgcgt ccttccagat cgggtcgccg ggcacgaagg cctcgggaaa caccacgagc
      781 ttggcgccgt tggcggcagc ctccccggtc agtgccgcaa ccctgtcgat cgtggcatct
      841 cgatccatca ggacgtacgc ggcttggact gccgcgaccg tgaacgcagt catgaagtga
      901 tcctccccgt caatccggcg ttgcgtgcac catcgtagtc gccgtcagag cgcgagcacg
      961 aaccgcagcg aggcgtccac ccgggtcatc gcctctggtc ccagccgccc gagccgagca
     1021 ccgagccacg tcgccgggcg cgtgaacacc tggtcgcact tcacgaaccc gtcgcgatcg
     1081 aggccagtgt cgcgcatggt cacgggcacg agatacggcg gcagccaccc gcccgcgacg
     1141 acccgccgct tcgtcgtcat cgagcacacc agaaccgtgc cgtcggcgcg gttcaggcga
     1201 tcggaggaga cgacgaccgc cggatgcggc ccgcggagca cgtggccgcc gacgtcctcg
     1261 aactggacga ggtggatgtc accgcgacgg gggccggcta ccactcgacg tccgcgagga
     1321 gcttcgcgtc catcggcgcg gtggcccgtg cccactcgag gttctcctcg cggtccgcct
     1381 cgagcgccgc gtcgagcctg gcctgctcga cctcgtccac gagccgccgg agcgacgccg
     1441 cgaccacgcc gctcaccgac gtcccggtct cgtccgcgac ttccttcacc gtcgccagga
     1501 gccgctcggg cacgctgatc gagagcttcg cgtaggggcc ggattcggtc ggcgtcaggt
     1561 agggctcgag cgcctcgcgg aaggtcggga agggtcgctc accgtggtcg gactgctggt
     1621 cgtactgcat ctcacggcga gtatgatcca tggtcgtacc cgggtcaagg accgccgcag
     1681 ccaggttccg tgggggagcc tcccacccgc ggcgtagagt gctgctcatg gcgacgaggg
     1741 atgcctgagc ccggggcggg cgccgacctc ctcgcgatgg gccgcgaggc gttcctgcgc
     1801 gagcagtggg ccgacgccta cgcccggctg acggaggccg atcagggtca gtcgctgggc
     1861 ctcgacgacc tggagctcgc tgcgctcgcg gcgtacctcg tgggcctcga cgaggcgtcc
     1921 gtcgacctcc agacccgcgc ctatgccgag gcattgcgtg gcggcgacgt cgaccgagcg
     1981 gcgcgccgcg ccttctggct cgggttcggg ctgttttacc ggggagagcg ggtcaggggc
     2041 ggtggctggc tggcccgggc agcgcgcctt gcccacgagg ccggacccga gtcgctcgtg
     2101 cacggctacc tgctcgtgcc accggccctg gtagaccacg aggccgggga ctccgaggcc
     2161 gcgtccgtcg cctttcggga ggccgcccgc gtcgcagcca agcacggaga cgccgacctc
     2221 gagaccatcg cccggctcgg gctgggcgac gcactgatcg gcctcggcca ggtctcgcac
     2281 ggcatggccc tgctcgatga ggccatggtg tcggtcacgg caggcgaggt gtcgccgatc
     2341 gtcatgggga tcgtctactg cgccgtgatc gagacctgcc agcggatctt cgacctcggt
     2401 cgcgcccacg agtggacggc ggcactggcg ggttggtgcg acgcccatcc cgacctcgtg
     2461 ccataccgtg gcgcctgtct cgtgtaccgg gccgacctga tgacgctcca cggcgcatgg
     2521 ccggaggccc tcgccgaggc gcggcgtgcc tcggacgcat cgcagcgacc cagcgtcgat
     2581 ccggtctcgg gcgaggccgc gtaccggcgc gcggagctgc accggctgcg tggcgaggcc
     2641 accgaggccg aggaggcgta tcgacatgcc aaccggctgg gacgcacgcc ccagccggga
     2701 ctcgccctgt tgcggctcga ccagggccgc gtggccgtgg ccgcatccgc gatccgccga
     2761 gccgtggacg gctccaccga cgtcgccgcg cgcccgcgac tgctcgacgc gttcgtggag
     2821 atctcgctgg cggccggaga cgtcgccgcg gccggcgctg cggccgacga gctggcgacg
     2881 ctcgcgggcg agctcgacgc gccggtgctc gtcgcgctcg ccgcccgggc cgagggcgcg
     2941 gtcctgctgg ccgaaggcga ggtgaagtcc gggctcgacg agctccaacg cgcgctcgcc
     3001 gcgtggcagg cggtagaggc gccgtacgag gcggcgcgtg tccgggtgct cgtcgcccag
     3061 gcctgctacc ggctgggtga cgatgacacc gcggacctgg agctcgacgc cgccgatcgc
     3121 gcgttccgcg acctgggggc cgcgcccgcc cgtcgacgcc tcgaggctct tcgggacggg
     3181 cgggcgtcgg ggggacgcgc gaacgccggc ggcctgacgc cgcgtgagct cgaggtcctg
     3241 cgccagctgg cctcgggcaa gacgaaccgc gcgatcgcca cggatctcgt catcagcgag
     3301 aagaccgttg cgcgccacgt cgccaacatc ttcaccaagc tcgatctctc gagccgctcc
     3361 gcggcgaccg cgtacgcgta cgagcacgac ctggtggcgc cggctacata gaaccaccca
     3421 tgccggtcgc gcctgcaatt ggatggttcg gtcgatgcgg tgccaacccg cccgccccta
     3481 gggtcgtgcc cggcgaccgg gcgatccggt cgcctccgca cccgacatcg gtgcccggag
     3541 gcgagacatg caggacggcg agcggttcga cgcgatcgtg atcgggggtg gccaggccgg
     3601 cctcgccgct ggctaccacc tgcgtcggca cgggctcaga ttcgtgatcc tcgatgccgg
     3661 tgcgcacgtc ggcgatgcct ggcggtcccg atgggacagc ctgcgcctgt tcacgccggt
     3721 ccgctacgac agc
//
LOCUS       DBNHXO010000491         1648 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1943920, whole genome shotgun sequence.
ACCESSION   DBNHXO010000491 DBNHXO010000000
VERSION     DBNHXO010000491.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1943920"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="AC162A_09930"
     CDS             complement(<1..320)
                     /locus_tag="AC162A_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HIR5011205.1"
                     /translation="MTRSFDVVAPPAAVVPGSLGVFGGTFDPVHHGHLALAEEACDAL
                     GLASVTFVPAAEPPHKPGTPVTPAEHRLAMVEAAIAGNPRFGCTRIELDRAGPSYTVD
                     TLAD"
     gene            complement(317..1606)
                     /gene="obgE"
                     /locus_tag="AC162A_09935"
     CDS             complement(317..1606)
                     /gene="obgE"
                     /locus_tag="AC162A_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR5011206.1"
                     /translation="MFLDDVRITVRAGAGGDGAATFRREAHVPRGGPDGGDGGRGGSI
                     YLRVDVGETTLRDYRYKHQFRATSGGRGERSRRHGKAGEDLFLTVPPGTGVLDSATGA
                     LIADLVAVGQEVMVAKGGRGGLGNTHFKTATHQAPTHAQRGEPGEERTIHLELRLIAD
                     VGLVGLPNAGKSTLLAALTAATPKIAAYPFTTLEPNLGVMDLGIEDGRRPTIADVPGL
                     IEGASSGAGLGHAFLRHVERTRILLHIVDGSSRDPRWDHDVIRDELGAHDPALLEKPM
                     VVAFNKMDLEAARDAWPTFRREALASGLQPLAISADTGEGLDELRAAVAALLPDAAGL
                     VEPPEPAGVVVHRLEAAGNAFAVAREDEAFRVSGKRIERLVAQTNFDNEESAERFQRE
                     LVRSGIDGALRKAGVRAGDAVRIGGSELEWDPPEDDR"
BASE COUNT          212 a          614 c          604 g          218 t
ORIGIN      
        1 atgtccgcca gcgtgtcgac ggtgtacgag ggtccggccc gatcgagctc gatccgggtg
       61 cacccgaacc tcgggttgcc ggcgatcgcc gcctccacca tcgccaaccg gtgctctgcc
      121 ggcgtcaccg gggtcccggg cttgtggggc ggctcggccg ccggcacgaa cgtgacggag
      181 gcgagaccca gcgcgtcgca cgcctcctcc gcaagcgcga ggtgaccgtg gtggaccggg
      241 tcgaacgtcc cgccgaagac acccagtgat ccgggcacca ccgccgccgg cggcgccacg
      301 acatcgaagg accgcgtcaa cggtcatcct cgggcggatc ccactcgagc tcagacccgc
      361 cgatgcggac cgcgtcgccg gcgcgaacgc cggccttgcg cagcgcaccg tcgatccccg
      421 accgaacgag ctcgcgctgg aagcgctccg cggactcctc gttgtcgaag ttggtctgcg
      481 cgacgagccg ctcgatccgc ttgccggaca cccggaacgc ctcgtcctcg cgcgccaccg
      541 cgaacgcgtt gcccgccgcc tcgagccggt ggacgacgac cccggcgggc tccggtggct
      601 cgacgaggcc ggcggcgtcg ggcaggaggg cggcgaccgc cgcgcgcagc tcgtcgagcc
      661 cctcgcccgt gtcggcggag atcgccaggg gctgcaggcc cgaggcaagg gcctcgcgcc
      721 ggaaggtcgg ccaggcgtcg cgcgccgcct cgaggtccat cttgttgaag gcgacgacca
      781 tcggcttctc gagcagggcc gggtcgtgcg cgcccagctc gtcgcggatg acgtcgtggt
      841 cccagcgggg gtcgcgcgac gagccgtcga cgatgtgcag caggatccgg gtccgctcga
      901 cgtgccgcag aaaggcgtgc ccgaggccgg cgccggacga cgccccctcg atcagccccg
      961 gcacgtcggc gatcgtcggg cgccggccgt cctcgatgcc caggtccatg acgcccaggt
     1021 tcggctccag cgtcgtgaac gggtaggccg cgatcttggg tgtcgccgcg gtgagggccg
     1081 cgagcaacgt cgacttgccg gcgttcggca ggccgaccag cccgacgtcg gcgatcagcc
     1141 gcagctcgag gtggatcgtg cgctcctcgc ccggctcgcc tcgctgcgca tgcgtcggcg
     1201 cctggtgggt cgcggtcttg aagtgggtgt tgccgaggcc accgcgaccg cccttggcga
     1261 ccatgacctc ctggccgacg gcgacgaggt cggcgatcag cgcgccggtc gcggagtcca
     1321 gcacgcccgt gcccggcggc acggtcagga acaggtcctc gcccgccttg ccgtgccggc
     1381 gcgatcgctc gccgcgcccg cccgaggtcg cgcggaactg gtgcttgtag cggtagtcgc
     1441 ggagcgtcgt ctcgccgacg tcgacgcgga ggtagatcga gccgccgcgc ccgccgtcgc
     1501 caccgtcggg gccgcccctc gggacgtggg cttcgcggcg gaaggtcgcc gcgccgtcgc
     1561 cgccggcacc cgcacgcacg gtgatccgga cgtcatcgag gaacattgcg gtgattgtcg
     1621 cacggggtcc gccagacccc gccgaggt
//
LOCUS       DBNHXO010000492         1547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1745948, whole genome shotgun sequence.
ACCESSION   DBNHXO010000492 DBNHXO010000000
VERSION     DBNHXO010000492.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1745948"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="AC162A_09940"
     CDS             complement(<1..925)
                     /locus_tag="AC162A_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR5011207.1"
                     /translation="MNIRRLAASAAVTAVFVGACSSGGGGATTAPSAAAPSGGAAGCK
                     VGVSWNNFKEERWAKWDEPAIKEVVEAAGGTYVGNDAGSSAEKQATNVEQLITDGAKV
                     IIILAQDGTAIQPTVQAAVDRGIPVIAYDRLIENEKAFYITFDNPLVGQLMAEEIIKV
                     VPKGNYVVIKGNSADANSDFLRSGIEKVIGESVKSGDIKIVGESYTDNWDAAKAQATM
                     EQYLTAEDNKIDAAVVLNDGMAGGVVAALKGQGLDGKVPVTGQDADKAALNRVALGTQ
                     LVSVGKDARVLGAAAGKAAVALCGGAALADVP"
     gene            complement(1083..>1547)
                     /locus_tag="AC162A_09945"
     CDS             complement(1083..>1547)
                     /locus_tag="AC162A_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR5011208.1"
                     /translation="GARCRCGSTGCWETEVGEGVLLALAGHPADAGRAGIDAVLREAE
                     AGSEAALRAIDHVGRWLGIGLGGIVNILNPRLIILGGPHARLHPLVRARLEVELNRHA
                     LPASRALVDVVPAALGVNAPLVGASELAFEPLLADPAAFFGGRDHTLHLASA"
BASE COUNT          215 a          533 c          535 g          264 t
ORIGIN      
        1 cggggacgtc ggccagtgcc gcgccgccgc agagggcgac ggcggccttg ccggccgcgg
       61 cgccgagcac gcgggcgtcc ttgccgacgc tgacgagctg ggtgccgagc gcgacacggt
      121 tcagggccgc cttgtcagcg tcctgtcccg tgacgggaac cttgccatcg aggccctggc
      181 ccttgagcgc cgcgacgacg ccgccggcca tgccgtcgtt gagcacgacg gctgcatcga
      241 tcttgttgtc ctcggcagtg aggtactgct ccatggtggc ctgcgccttg gcagcatccc
      301 agttgtcggt atagctctcg ccgacgatct tgatgtcgcc ggacttgacc gattcgccga
      361 tgaccttctc gatgcctgag cgcaggaagt cggagttcgc gtcggcgctg ttgcccttga
      421 tgacaacgta gttgcccttg ggcacgacct tgatgatctc ctcagccatc agctggccga
      481 cgagcgggtt gtcgaaggtg atgtagaacg ccttctcgtt ctcgatcagg cggtcatacg
      541 cgatcaccgg gatgccccga tcgacggcgg cctgcacggt cggctggatc gccgtgccgt
      601 cctgggccag gatgatgatc accttcgcgc cgtcggtgat gagctgctcg acgttcgtcg
      661 cctgcttctc agcggacgat cctgcgtcgt tgccgacgta ggtgccgccc gcggcctcga
      721 cgacttcctt gatggcgggc tcgtcccact tggcccagcg ctcttccttg aagttgttcc
      781 acgacacgcc gaccttgcag ccggcggctc cgccgctcgg tgctgcagcg ctcggtgccg
      841 tggtggcccc gccgccgcct gacgaacagg ctccgacgaa gacggcagtg acggccgcgg
      901 atgcggccag cctgcgaatg ttcatgcgct cgatctcctc ctccagcgac gacggacgtc
      961 gtcgtcattt cagtgcgcag cttccggcgc gtaggctaca tcgttaggca atcccctttc
     1021 atgccggtcg ccgtttttca cggcggttcg tgccgtcgat ggaccgatcg cgagcaccca
     1081 cgtcaggcac tcgccaggtg tagcgtgtgg tcgcggcccc cgaagaaggc cgccggatcg
     1141 gcgagcaggg gttcgaacgc gagctccgac gcgccgacga gcggggcgtt gacgccgagg
     1201 gcggccggca cgacgtcgac cagggcccgg gacgccggga gggcgtgccg attgagctcg
     1261 acctcgagcc gggcgcgcac cagcgggtgg aggcgcgcgt gcgggccgcc aaggatgatg
     1321 aggcgtgggt tgaggatgtt gacgatcccg ccgaggccga tgcccagcca gcggcccacg
     1381 tgatcgatag cccgcaacgc cgcctcggag ccggcctcgg cctcgcgcaa cacggcatcg
     1441 atcccggccc ggccggcgtc ggcgggatgg cccgccagcg cgagcaggac gccttcgccg
     1501 acctcggtct cccagcagcc ggtcgaaccg cagcggcagc gggctcc
//
LOCUS       DBNHXO010000493         2347 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1550400, whole genome shotgun sequence.
ACCESSION   DBNHXO010000493 DBNHXO010000000
VERSION     DBNHXO010000493.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1550400"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..287)
                     /locus_tag="AC162A_09950"
     CDS             complement(72..287)
                     /locus_tag="AC162A_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011209.1"
                     /translation="MPGIVVPVFVIFIVVAISGAGVVVVPVVVVAVSGARVAVVAVIV
                     VAVARTRAVVVTTVVVIVRRRAGGGAW"
     gene            350..895
                     /locus_tag="AC162A_09955"
     CDS             350..895
                     /locus_tag="AC162A_09955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5011210.1"
                     /translation="MDDRALVDAARGGDRDAFGRIVERESAVVYRTCLRILGRPQDAE
                     DVTQESFVAAYRAIGGYRGEGSMRGWLLRIAQRQAYRRLEQRRPTAELDSVGEARLAD
                     ASADPTRVVAAIEARTEIRDAVAALPEPYREVVSLRFFGELSLFEVAEATGRPLGTVK
                     THLRRGLERLRPIVGGGGADR"
     gene            892..1497
                     /locus_tag="AC162A_09960"
     CDS             892..1497
                     /locus_tag="AC162A_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011211.1"
                     /translation="MTHHPYGPDELDRADPELDEVAHAIERYALDHDGKMPAGLPSRI
                     IAAIDMEPDPEPRWWAVLLAGPRAWRPAGRLAAMAAVIAIATVGAVALGAIVEGVRPS
                     VGTSSPPSPIISPSEAPTDAVTPSPTPSLSSSPSSSATPSSLQASPAPSPTVVPATAV
                     PTASDDDDEDETPEPSESDNSGPGGGGGDDDSGPGGGDDGG"
     gene            complement(1500..>2347)
                     /locus_tag="AC162A_09965"
     CDS             complement(1500..>2347)
                     /locus_tag="AC162A_09965"
                     /inference="COORDINATES: protein motif:HMM:NF048867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Kelch repeat-containing protein"
                     /protein_id="HIR5011212.1"
                     /translation="EVAYLFGGRDGSVSFADLWVYDLAADAWTELAPASPPPARFGHE
                     AAWVDGVGLVVFGGQAGTAFFNDLWAYDAAANAWSQLPSDGDVPVPRYGSCSAVGPDD
                     RLWISHGFTSDGARFSDTLAYDFVSRQWSDETPGGEHPVERCLHACWVSDAGELTLYA
                     GQTTGVAALGDRWTLGAAGWREVAGVLPPERNLPAHARIDGATVVFGGMGLDAAFLDD
                     LWLLGDEEADGEMLTPGGETPAPRSGASMVTDTVRDRVLLFGGRGADGAFGDTWDLVG
                     VDGGL"
BASE COUNT          375 a          837 c          842 g          293 t
ORIGIN      
        1 gcgatgacga cgatcgagcg cccgggagtg cctcgcccag cgcgtcggct gacgacggat
       61 cgggcagcgc gtcaccaagc gcctcccccg gcgcgtcggc ggacgatgac gacgaccgta
      121 gtgacgacga cagctcgggt ccgggcaacg gcgacgacga tgaccgcgac gacggccact
      181 cgggccccgg aaacggccac gacgacgacg gggacgacga caactccggc cccggaaatg
      241 gcgacgacga tgaagatgac gaagacgggg acgacgattc cgggcacggc ggctgagccg
      301 attggcgcgc tcgctctgga tgcagcccgc gacatggcac gatgggggcg tggatgaccg
      361 cgccctcgtc gacgccgctc gaggcggcga ccgcgatgcc ttcgggcgga tcgtcgagcg
      421 tgagtccgct gtcgtgtatc ggacctgcct gcggatcctc gggcgaccgc aggacgccga
      481 agacgtgacc caggagagct tcgtggccgc gtatcgggct atcggcggct accgcggcga
      541 aggttcgatg cgcggatggc ttctgcgcat cgcgcagcgc caggcgtacc gacgtctgga
      601 gcagcgtcga ccgacggccg agctcgactc ggtcggcgaa gcccgcctgg ccgatgcgtc
      661 cgcggacccg acgcgggtcg tggccgccat cgaggcgcgc accgagatcc gggacgccgt
      721 cgcggcgcta cccgagccgt accgggaggt cgtttcgctc cgcttcttcg gcgagctgtc
      781 gctgttcgag gttgccgagg ccaccggtcg cccgctgggc acggtgaaga cccacctgcg
      841 ccgcgggctg gagcgtctgc ggccgatcgt cggcggaggg ggagccgacc gatgacgcac
      901 cacccctacg gtcccgacga gctggaccga gccgatcccg agctcgacga ggtcgcacac
      961 gcaatcgagc gctacgccct ggaccacgac gggaagatgc ccgctgggct cccgtcgcgg
     1021 atcattgcgg ccatcgacat ggagcccgac ccggagcccc gatggtgggc cgtgttgctg
     1081 gccggaccac gtgcgtggcg gcccgccggc cgtctcgcgg ccatggcggc cgtgatcgcc
     1141 atcgccaccg tcggtgcggt ggcgctgggc gcgatcgtgg agggcgtgcg tcccagcgtt
     1201 ggcacgtcct cgccgccgtc cccgatcatc agcccgagtg aggccccgac cgacgccgtg
     1261 actccgtcgc cgacgccgag cctttcatcg tcgccatcga gttcggcaac gccgtcatcc
     1321 ctccaggcca gcccggcgcc gagtcccacg gtggttccgg cgacggcggt tcccaccgcc
     1381 tcggacgacg atgacgagga cgagacgccc gagccgagcg aatcggacaa cagcggacca
     1441 ggcggcggag ggggtgacga cgacagcggc cccgggggcg gcgacgacgg aggctgaggc
     1501 taaagtcccc cgtcaacgcc gacgaggtcc caggtgtcgc cgaacgcgcc atcggccccc
     1561 cggccgccga acaggaggac gcggtctcga acggtgtcgg tcaccatcga ggcacccgac
     1621 cgtggcgcgg gggtctcacc gccgggcgtg agcatctccc catcggcttc ctcgtcgccc
     1681 agcagccaga gatcatcgag gaaggcggcg tcgagtccca tgccgccgaa gacaaccgtc
     1741 gctccgtcga tgcgcgcatg agccggcagg ttgcgctccg gcggcagcac cccggcgacc
     1801 tcgcgccacc cggccgcacc gagagtccat cggtcgccga gagccgcaac cccggtcgtt
     1861 tgcccggcgt acagggtcag ctcgcccgca tcggataccc agcaggcgtg gaggcagcgc
     1921 tcgaccgggt gctcgccgcc cggtgtctca tcgctccatt ggcgtgagac gaagtcgtag
     1981 gccagcgtgt ccgagaagcg ggcaccatcc gaggtgaagc cgtggctgat ccataggcgg
     2041 tcatcgggac ccaccgcgct gcacgagccg tagcggggca cggggacgtc gccgtctgac
     2101 ggcagctgcg accaggcgtt tgccgctgcg tcatacgccc acaggtcgtt gaagaaggcc
     2161 gtgccagcct ggccgccgaa cacgacgagt ccaacgccgt ccacccacgc cgcctcgtgt
     2221 ccgaagcgag ccggcggcgg gctggccgga gccagctcgg tccacgcgtc ggccgcgagg
     2281 tcatagaccc acaggtcggc gaagctcacg gagccgtcgc ggccaccgaa gagataggcg
     2341 acctcag
//
LOCUS       DBNHXO010000494         6571 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1550508, whole genome shotgun sequence.
ACCESSION   DBNHXO010000494 DBNHXO010000000
VERSION     DBNHXO010000494.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1550508"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="AC162A_09970"
     CDS             complement(<1..816)
                     /locus_tag="AC162A_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5011213.1"
                     /translation="MSSPFARAVETIAPARLGRGFRWLLAASTLTNIGDGVALAAGPL
                     LVASLTQDPLLVSLTLLSQTLPPLLFGLFAGVVADRHDRRRIVAVVNVVRAVVLGLLA
                     TTIVSDAASIPIVLAALFTLATAETFADVGSSSLLPRLVRREDLGIANARLSGATLLT
                     NQLVAPPIGAALFVVGMALPFATDAACFALGAILVLRIAHEPAEARPGGRSTIRADLA
                     EGLRWLLSHPAMRTLALTIVAFNITFGAAWSVLVLYASERLGMTAVGYGLLTTA"
     gene            complement(878..4438)
                     /locus_tag="AC162A_09975"
     CDS             complement(878..4438)
                     /locus_tag="AC162A_09975"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5011214.1"
                     /translation="MSICGGCGTENEVGRKFCGECGSRLAAVCPACGTTNAPTAKFCG
                     ECASPLVAGLVAPADRPLPAGPAAATAAQAGPASPVAERRLVSILFADLVGFTTLAEG
                     RDAEDTRELLSRYFDLSRDVVGRYGGTVEKFIGDAVMAVWGAPVAHEDDAERAVRAAL
                     ELVDAVKGLGTGIAARAGVLTGEAAVTIGATNQGMVAGDIVNTASRLQSVAAPGTVLV
                     GEGTERAASRAIAFEAAGEQALKGKAAPVPAWRAVRVIAQVGGRNRSETLEAPFVGRD
                     DELRLLKDLYHATAREQRARLVSVIGPAGIGKSRLAWEFRKYEDGLVETVWFHEGRSP
                     AYGDGISFWALGEMVRRRAGLLETDDDQTTRVKVAATVAEHVPDPAERRWIEQALLAL
                     LGLETGGTASDELFAAWRMFFERLAATAPVVLVFEDFHHADSGLIDFVEHMLEWSRTF
                     PIYILTLARPDFLERRADWGAGKRSFTSIFLEPLAPDAMQALLAGLVPGLPQSAARAI
                     VERADGIPLYAVETVRMLLSEGKLTLSGGAYQPTGDLTSLAIPETLTALIASRLDGLS
                     PADRALVSDAAVLGQSFTIGGLTSVSGLEEEELAGRLRALVTREILTLQTDPRSPERG
                     QYAFVQALIREVAYNALAKRDRKVRHLAAARFFESLDTDELAGGLAGHYLAAYRNSAE
                     GPEADAVGAQARIALTAAAHRAIDLGSFDQAQTFLEQALAVTTDKGETAALLERAGEA
                     ASAAARREAAEAHLRRAISEYRELGELTGIIRATAALGRCILDSGRVADALAVLEPAV
                     IEFGDLPFGHGRIALEGQLARAYFFADDNRRAVEIADRVLDAAEHADLAAVVADTLVT
                     KGTALATLGRPIEGLGAIAAGRELAEASGLGVTVLRADNNRAFSLAGRDPRAGLAASR
                     DGLALARRLGARGWVPGMIQNLGEFGMRTGDWAMAMAELTAALAEDFEGSDLGGLLVS
                     YVQIAALRGDPIESRLDEFRLLGGDEAFPQEMANRAVAKGFSAFASGDLATARAAWLE
                     AAAAAAAEAPTSVPRAARAALWSGDVAGAIDDVRLLEQSELHGAAFQADLMTVRAGVA
                     ALEGRRADAMSLYRDSLRAWRDLGLAWDEALCELDMVLLLGPDDPEIRAAGEAARQIL
                     ARLEAAPILARLDTALDTTGRAVAARQRPVDQLVGDAARA"
     gene            complement(4512..5273)
                     /locus_tag="AC162A_09980"
     CDS             complement(4512..5273)
                     /locus_tag="AC162A_09980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HIR5011215.1"
                     /translation="MTKTTTESGGHAPSGAATEAGNAAPTPEVSAMFDRIARVYDPMN
                     LAISAFQEPYWRGRLVRSTGLQPGMRALDVASGTGKVAADLHRRVQPGGRVLGVDISP
                     GMIEIAQRQYGVREGLDYVVGDALALPTEDDSFEAATIAFGMRNLPDYRRGFEEMARS
                     VRPGGRVLCLEIARPRSRLAKFMRWWFDRIVPLIGRVAGQGDAYGYLVRSVRAYPAPE
                     RVAEIMREAGLVDVHWRGLTGGIVTLHVGTVPRRS"
     gene            complement(5270..>6571)
                     /locus_tag="AC162A_09985"
     CDS             complement(5270..>6571)
                     /locus_tag="AC162A_09985"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5011216.1"
                     /translation="PGVTIAIETPDADGIGEILVTTPARFEGYAGEPRGDGPIRTGDL
                     GRLDDERRLYVVDRRTDRIVRGGENVAPAEVEAVLIEQPAIADAAVVARRDDVLGQVP
                     VAAIVVRVDATDPGDDAFLRACRDRLAGFKVPASIVRLDALPRTAGGKLRRDAVRALV
                     DGASAGILARPEGDEIGWRVTGSGPQAVVLLPGTLSNAAQLDRLAGELAQPADLTIHA
                     IDRRGMGTSRLARPVVLDVDIHVRDLVAYLDARGIDRAIVVGVSFGGVVALELAARHP
                     ERILALVAWEPPYGPLADPSTQARFATLAAETANAHKKGGPAASAETFLRAVAGDAAW
                     DRLPERARAFLAREGDGALTDAALVGLDPDGLAHIEAPTTILTGSASERFYAPIADAL
                     ASRIPAARRDTLDGLAHPAPITEPARFAGAVRQSIAEEARS"
BASE COUNT          957 a         2392 c         2391 g          831 t
ORIGIN      
        1 cgccgtcgtc agcagcccgt agccgacggc cgtcatgccg aggcgttcgc tcgcgtagag
       61 gaccagcacg gaccacgcgg cgccgaacgt gatgttgaac gcgacgatgg tgagcgcgag
      121 cgtgcgcatc gcggggtgcg agagcagcca tcgcagtccc tcggcgaggt cggcacggat
      181 cgtcgaccga cccccgggcc gcgcctcggc cggctcgtgc gcgatccgga gcacgaggat
      241 cgcgccgagg gcgaagcagg ccgcgtccgt cgcgaacggg agcgccatgc cgacgacgaa
      301 cagcgcagcg ccgatgggcg gggcgacgag ctggttcgtc agcagggtcg cgcccgaaag
      361 ccgggcgttc gcgatgccca ggtcctcacg ccgcacgagc cggggcagga ggctgctgct
      421 gccgacgtcg gcgaaggtct ccgcggtggc cagggtgaac agcgcagcga ggacgatggg
      481 gatgctggcg gcgtcgctga cgatcgtggt cgccagcagc cccaggacca cggcccgcac
      541 gacgttgacc accgcgacga tccgccgccg gtcgtgccgg tcggcaacga caccggcgaa
      601 cagcccgaac agcaggggcg gcagcgtctg gctgagcagc gtcagggaga cgagcagcgg
      661 atcctgcgtc agcgaggcga cgagcagcgg accggcggcg agcgccacgc cgtcgccgat
      721 gttcgtcagc gtggaggcgg cgagcagcca gcggaagccc cggccgaggc gggccggggc
      781 aatggtctcg acagcgcggg cgaacggcga tgacacgccg cgagagtacc ggcggctcag
      841 tggcggggga ccatccccac gggtgcggat cccccggtca tgcccgtgcg gcgtcgccga
      901 ccagctgatc cacgggccgc tgccgggcgg cgaccgctcg gccggtcgtg tccaatgccg
      961 tgtcgaggcg cgcgaggatt ggggccgcct cgaggcgcgc gaggatctgg cgggcggctt
     1021 cgcctgcggc acggatctct gggtcgtcgg gcccgaggag gagcaccata tcgagctcgc
     1081 acagcgcttc gtcccaggcg agcccgagat cacgccaagc tcgcaacgaa tcgcggtaga
     1141 gcgacatcgc gtcggcccgg cgaccctcca acgccgcgac tccggcccgc acggtcatga
     1201 ggtctgcctg aaacgcggcc ccgtggagct cggattgctc gaggagcctg acgtcgtcga
     1261 tggccccggc gacgtcaccc gaccacagtg cggcccgggc ggcccggggc acggacgtcg
     1321 gcgcctcagc ggccgcggcc gcggccgcct ccagccacgc ggcccgcgcg gtcgccaggt
     1381 ctccgctcgc gaaagccgag aatcccttgg cgacggcccg gttcgccatc tcctgcggga
     1441 aggcttcgtc accaccgagc agccggaatt cgtcgaggcg tgactcgatg gggtccccgc
     1501 gcagcgctgc gatctgcacg tagctcacga ggagcccacc caaatccgag ccctcgaagt
     1561 cctccgcgag cgccgcggtg agctccgcca tggccatcgc ccagtcgcct gtacgcattc
     1621 cgaactcgcc gaggttctgg atcatgcccg gtacccagcc ccgggcaccg agccgccgcg
     1681 cgagggcgag gccgtcacgc gacgccgcga gccccgcgcg gggatcccgc cccgccagtg
     1741 agaaggcgcg gttgttatcg gccctcagca ccgtcacccc caacccgctc gcctccgcga
     1801 gctctcgacc cgcggcgatc gcgcccaaac cctcgatcgg ccggccgagc gtggcgaggg
     1861 ccgtgccctt cgtcaccagc gtgtccgcga cgacggccgc gaggtcggcg tgctcggccg
     1921 catccaggac gcggtccgcg atctcgaccg ctcggcggtt gtcgtcggcg aagaagtagg
     1981 cacgcgcgag ctggccctcg agggcaattc ggccatggcc gaacgggagg tcgccgaatt
     2041 cgatgacggc cggctccagg acggccaggg catcggcgac ccggccggag tcgaggatgc
     2101 accgacccag tgcagcggtt gcccggatga tccccgtgag ctcgccgagt tcgcgatact
     2161 ccgagatcgc gcgccggagg tgcgcctcgg ccgcctcccg ccgcgcggcg gccgacgccg
     2221 cttcgccggc acgctcgagc agggcagccg tctcaccctt gtccgtggtc acggccagcg
     2281 cctgctcgag gaaggtttgc gcctggtcga aggatccgag gtcgatggca cgatgcgccg
     2341 ccgccgtgag cgcgatccgc gcctgcgcgc ccaccgcatc ggcctcgggg ccctccgcgc
     2401 tgttccggta tgccgccagg tagtggccgg caagccctcc cgcgagctcg tccgtgtcga
     2461 ggctctcgaa gaagcgcgcc gccgcgaggt ggcgcacctt gcggtcgcgt ttggcgaggg
     2521 cgttgtaggc gacctcccgg atcagcgcct ggacgaaggc gtattgaccc cgctcggggc
     2581 tccgcgggtc tgtctggagc gtgaggatct cgcgggtaac cagcgcacgc aggcgtccgg
     2641 cgagctcctc ctcctcgagg ccactgaccg atgtaagtcc accgatcgtg aagctctggc
     2701 cgagaacggc ggcgtcggac accagcgctc gatcggccgg cgacagcccg tcgaggcgag
     2761 acgcgatgag tgccgtcagc gtctccggga tcgccaggct cgtcaggtcg ccggtgggct
     2821 gataggcacc cccgctgagc gtcagcttcc cctcggacag gagcatccgc acggtctcga
     2881 ccgcgtacag cgggatcccg tccgcccgct cgacgatcgc gcgcgcggcg ctctgcggca
     2941 ggccgggcac gagacccgcg aggagcgcct gcatcgcatc gggcgcgaga ggctcgagga
     3001 agatcgaggt gaagctgcgc ttgccagcgc cccagtccgc cctccgctcg aggaagtccg
     3061 gccgcgcgag cgtgaggatg tagatcggga aggtgcgcga ccactccaac atgtgttcga
     3121 cgaagtcgat cagcccgcta tcggcgtggt ggaagtcctc gaacaccagg acgaccggcg
     3181 ccgtcgccgc caggcgctcg aagaacatgc gccatgcggc aaacagctcg tcgctcgcgg
     3241 tgcccccagt ctcgagccca agcagcgcga gcagggcctg ctcgatccag cgacgctcgg
     3301 ccgggtcggg cacgtgctcc gcgaccgtcg cagccacctt cacgcgtgtc gtctgatcgt
     3361 cgtccgtctc gagcaggccg gcccggcgcc ggaccatctc gcccagcgcc cagaagctga
     3421 tgccgtcgcc gtaggccggc gagcggccct cgtggaacca gaccgtctcg acgaggccgt
     3481 cttcgtactt ccggaactcc cacgccagcc gggacttgcc gattcccgcc ggcccgatca
     3541 cggacaccag gcgcgcgcgt tgctcccggg cggtcgcgtg gtacaggtcc ttgagcagcc
     3601 gcagctcatc gtcacggccg acgaacggcg cctcgagcgt ctcggaccgg ttccgcccac
     3661 cgacctgcgc gatgacgcgg accgcccgcc aggctgggac gggcgcggct ttccccttga
     3721 gcgcctgctc gccggcggcc tcgaaggcaa tcgctcggga cgccgcccgc tcggtccctt
     3781 cgccgacgag gacggtcccc ggcgccgcga ccgactgcag gcggctggcc gtgttcacga
     3841 tgtcgccggc gaccatgccc tggttcgtcg cgccgatcgt cacggccgcc tcgcccgtca
     3901 ggaccccggc tcgagcggcg atgccggtgc caagcccctt caccgcgtcg accagctcga
     3961 gcgcggcccg gacggcacgc tcggcgtcgt cctcgtgggc gaccggagcg ccccagacgg
     4021 ccatgaccgc atcgccgatg aacttctcga ccgtgccgcc gtaacggccg acgacgtcgc
     4081 gcgacaggtc gaagtagcgg gacaggagct cgcgcgtgtc ctccgcgtca cgaccctcgg
     4141 ccagcgtcgt gaaccccacc aggtcggcga acaggatcga gacgagacga cgctcagcga
     4201 ccggcgatgc aggtccggcc tgtgcggccg ttgcagccgc ggggccggca ggcagcgggc
     4261 ggtcggcagg cgcgaccaac cctgcaacga gcggcgaggc gcactcgcca cagaacttcg
     4321 cggttggtgc gttggtcgtg ccgcatgctg ggcagacggc agcaaggcgc gagccgcact
     4381 cgccgcagaa cttgcgcccg acctcgttct cggtgccgca gccgccgcag atcgacaaac
     4441 acgtccctct tgacgtcagg ccgggagcgt accagtagcg ggtcggcgat cgcagccccg
     4501 gttcgatgcc atcacgaccg ccgggggacg gtcccgacgt ggagcgtgac gatgccgccg
     4561 gtcaggccgc gccaatggac gtcgacgagg ccggcctcgc gcatgatctc ggcaacccgc
     4621 tccggcgcgg ggtaggcgcg cacactgcgc acgagatagc cgtaggcgtc gccctggccg
     4681 gcgactcggc cgatcagcgg cacgatccgg tcgaaccacc agcgcatgaa cttcgcgagg
     4741 cggctgcgcg gccgggcgat ctcgaggcac agcacacgac ccccgggccg gacggagcgg
     4801 gccatctcct cgaaaccgcg ccggtagtcg ggcaggttgc gcatcccgaa ggcgatcgtg
     4861 gcggcctcga acgagtcgtc ctccgtcggc agggcgaggg cgtcgccgac gacatagtcg
     4921 agcccctcgc gaacgccgta ctgccgctgc gcgatctcga tcatcccggg cgagatgtcc
     4981 acgcccagca cccgaccgcc gggctggacc cgccggtgaa ggtcggccgc caccttgccg
     5041 gtccccgatg cgacatcgag cgcgcgcatg ccaggctgta gcccggtcga ccgcaccagc
     5101 cgcccgcgcc agtacggctc ctggaacgcg gagatcgcga ggttcatggg gtcgtagacc
     5161 ctcgcgatgc ggtcgaacat cgcgctcacc tcgggcgtcg gcgcggcgtt gccggcctcg
     5221 gtcgcggcgc cggacggcgc gtgccccccg gactcggtgg tggtcttcgt catgaacgcg
     5281 cctcctcggc gatggactgg cggaccgcgc cggcgaaccg ggcaggctcg gtgatcgggg
     5341 ccggatgcgc gaggccgtcg agggtgtcgc gccgggccgc ggggatccgg gaagcgaggg
     5401 cgtcggcgat cggcgcgtag aaccgctcgc tcgcggagcc ggtgaggatc gtggtcgggg
     5461 cctcgatgtg cgcgagcccg tcggggtcga gacccacgag cgcggcgtcc gtgagcgccc
     5521 cgtcgccctc gcgagcgagg aaggcgcggg cccgctccgg aaggcggtcc cacgcggcgt
     5581 caccggcaac cgcgcgcagg aacgtctcgg ccgaagcagc ggggccgccc ttcttgtgag
     5641 cgttcgctgt ctcggcggcg agggtcgcga agcgcgcctg cgtcgacggg tccgccagcg
     5701 ggccgtacgg cggctcccag gcgacgagcg cgaggatccg ctccgggtgg cgtgcggcga
     5761 gctccagcgc gacgacgccg ccgaagctga ccccgaccac gatcgcccgg tcgatcccgc
     5821 gggcgtccag gtaggcgacg aggtcccgga cgtggatatc gacgtcgagc acgaccggcc
     5881 gagcgaggcg ggacgtcccc atgccgcgcc gatcaatggc gtggatggtc aggtcggcgg
     5941 gttgagcgag ctcgccggca agccggtcga gctgggcggc gttcgacagc gtgccgggaa
     6001 gcaggaccac ggcctgtggc cccgagccgg tgacccgcca cccgatctcg tcgccctcgg
     6061 gtcgggcgag gatgccggca gaggcaccat cgacgagggc acggacggcg tcgcgccgga
     6121 gcttgccgcc ggcggtccgc ggcagcgcgt cgaggcggac gatcgaggcc gggaccttga
     6181 accccgcgag ccggtcgcgg caggcgcgga gaaaggcgtc gtcgccgggg tcggtggcat
     6241 cgacgcgcac cacgatcgcg gccaccggga cttggcccag gacgtcgtcg cggcgcgcga
     6301 ccacggcggc gtccgcgatc gccggctgct cgatcaggac cgcctcgacc tcggccggtg
     6361 cgacgttctc gccgccgcgc acgatccggt cggttcggcg gtcgaccacg tacaaccgcc
     6421 tctcatcgtc cagtcgcccg aggtcgccgg tccggatcgg gccgtcgccc cgcggttcgc
     6481 cggcgtagcc ctcgaaccgg gcgggcgtgg tgacgaggat ctccccgatg ccatccgcgt
     6541 cgggcgtctc gatcgcaatc gtgacccccg g
//
LOCUS       DBNHXO010000495         1812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4923818, whole genome shotgun sequence.
ACCESSION   DBNHXO010000495 DBNHXO010000000
VERSION     DBNHXO010000495.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4923818"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="AC162A_09990"
     CDS             <1..1035
                     /locus_tag="AC162A_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011217.1"
                     /translation="GGWPRLRIRTTRTGDTIRYREQLGALSAAFDVQLAPTAGGPVVL
                     DANWLLVWSHHVLYTNMVEPLLRFMLVARGYVLLHSAAVEAEQGAVVMSALTDTGKTS
                     TVLRLLMHRSWGFIADDMAIVAPGGVIIGFPKPMTLSSHTMSSVNEQALPLADRLMLG
                     IRSRLHSREGRAVGHSLGRMPVPIVTINAWVQLLIPPPKYHVTSLIDCDVVDRSTIDA
                     VILMERGEPLAEIPTLDATLDRLLANTDDAYTFPPFAALAPLLEFDGLDHDALRVRER
                     ALLSAAVDAAWRVRVRVRGHNWSDLIPTLVTDKRPVLEPDPLVVAQDELAIDDAPAKR
                     TPASAGTSVR"
     gene            1067..>1812
                     /locus_tag="AC162A_09995"
     CDS             1067..>1812
                     /locus_tag="AC162A_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR5011218.1"
                     /translation="MGKDRSRPEWRADLEVDEALVRRLLGAQFAGLETLTVVPFAEGW
                     DNALWLVNGELAFRFPRRAIAVPGVEQEIRLLPDLARRLPFPIPNPVYVGRPSDAFPW
                     PFFGAPLLSGSEPARVPIVEPRSALGAGLGAFLRTLHDPALAADLGGELPYDPMGRAD
                     MAVRVPRTRDRLNALQTAGLWTAPPEAERLMAAAYGLGRADGFALTHGDLHVRHLLVD
                     DDGSPSAVIDWGDVCLGDPSIDLSLYWSLL"
BASE COUNT          242 a          664 c          617 g          289 t
ORIGIN      
        1 ggcggctggc cgcggctgcg catcaggacg acccgaacgg gcgacacgat ccgctaccgt
       61 gagcagctgg gcgcgctctc ggccgccttc gacgtgcagc tcgcccccac ggcgggtgga
      121 ccggtcgtcc tcgacgccaa ctggctgctc gtctggtcgc accacgtgct gtataccaac
      181 atggtcgagc cgctgttgcg gttcatgctc gtcgcccgcg gctacgtgct gctccacagc
      241 gccgccgtcg aggccgaaca gggcgccgtc gtgatgtccg ccctcaccga caccggcaag
      301 acgagcacgg tcctgcgcct gctgatgcat cgttcgtggg gcttcatcgc ggacgacatg
      361 gcgatcgtgg cgcccggcgg ggtgatcatc ggattcccga agcccatgac gctctcgtcg
      421 cacacgatga gctcggtcaa cgagcaggcc ctgccgctgg cggatcggct gatgctgggc
      481 atccgctcgc gcctgcattc gcgcgagggc cgcgcggtcg ggcactcgct cggacgcatg
      541 cccgtgccga tcgtgaccat caacgcctgg gtgcagctgc tcattccgcc gcccaagtac
      601 cacgtgacgt cgctcatcga ttgcgacgtc gtggatcgat cgacgatcga cgcggtcatc
      661 ctcatggagc gaggcgagcc gctcgcggag atcccgacgc tcgacgcgac cctcgaccgg
      721 ctgctcgcca acaccgacga cgcctacacg tttccaccgt tcgcggcgct ggctccgctg
      781 ctcgagttcg acggcctcga ccacgatgca ctgcgggtgc gtgagcgcgc gctgctgtcg
      841 gcggccgtcg acgcggcatg gcgggtccgc gtccgggtcc gcggtcacaa ctggtccgac
      901 ctcatcccga ccctcgtgac cgacaagcgg cccgtcctcg agcccgaccc cttggtcgtc
      961 gcccaggacg agctcgcgat cgacgacgcg cccgcgaagc ggacgcccgc ctcggcggga
     1021 accagcgtcc gctgaggcgt cagcccgccg gcaatagggg atcccggtgg gcaaggaccg
     1081 atcacgaccc gagtggcgcg ccgacctgga ggtcgacgag gccctcgtcc gccggctcct
     1141 gggcgcgcag tttgcgggcc ttgagaccct gaccgtcgtc ccgttcgccg agggctggga
     1201 caacgcgcta tggctggtga acggcgaact cgccttccgc tttccgcggc gcgcgatcgc
     1261 cgtgccgggg gtcgagcagg agatccggct gctgccggac ctggcgagac ggcttccgtt
     1321 cccgatcccg aatcccgtct acgtcggccg gccctcggac gcctttccgt ggccgttctt
     1381 cggggcaccg ctcctgtcgg gatcggagcc cgcccgcgtc ccgatcgtcg agccacggtc
     1441 ggcgctgggt gccggtttgg gcgcgtttct tcggacgctc cacgaccctg cgctggcggc
     1501 cgacctcggc ggggagctgc catacgatcc gatgggccgc gccgacatgg cggtccgcgt
     1561 gccccgaacg cgcgacaggc tgaacgccct acagacggcg gggttgtgga cggcgcctcc
     1621 cgaggccgag cggctcatgg ccgcggccta cgggctgggg cgagcagacg gcttcgccct
     1681 cacgcatggc gacctccatg tccgccacct gctcgtcgac gacgacggca gtccgagcgc
     1741 cgtcatcgac tggggcgacg tgtgcctggg cgatccgtcg atcgacctct cgctctactg
     1801 gagcctgctt ga
//
LOCUS       DBNHXO010000496         2353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2144073, whole genome shotgun sequence.
ACCESSION   DBNHXO010000496 DBNHXO010000000
VERSION     DBNHXO010000496.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2144073"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="AC162A_10000"
     CDS             <1..683
                     /locus_tag="AC162A_10000"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5011219.1"
                     /translation="GPKPSMRKEIAIGLRYVTGHRWLRSIAATTGTANFFGNVMGAIL
                     ILYVTREGGLTAEAIGFAFSIGSVGVLLAALVTSRLTARLGVGRMLVLTSIGFSLSGL
                     LVAAASTTYLFFALALFGLLGGFASVAWNINQVSLRQAITPPRMQGRMNATMRFIVWG
                     TIPLGAIIGGILGDLIGLHATIWVGAIGGMFAFLPVATSSVRQIVRMPEPVDDDSSAG
                     SMADAEPA"
     gene            complement(698..>2353)
                     /gene="hutU"
                     /locus_tag="AC162A_10005"
     CDS             complement(698..>2353)
                     /gene="hutU"
                     /locus_tag="AC162A_10005"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HIR5011220.1"
                     /translation="GPRPVRAPRGTELSTHGWQQEAVLRMLMNNLDPEVAENPDALVV
                     YGGTGRAARSWEAFDAIVRELRRLADDETLLVQSGKPVAVFRTHEWAPRVLIANSNLV
                     GRWATWEHFRELERQGLMMYGQMTAGSWIYIGTQGILQGTFETFAELARQHFDGTLRG
                     RVTLTAGLGGMGGAQPLAVTMNDGVCLAIEVDETRARRRHEIGYVDRLTHSPDEALAM
                     ARSAAADGQAISIALVGNAAEIEPAWAAAGERFDVVTDQTSAHDALAGYVPAEISLED
                     AAALRAAQPELYEARAMRSMADHVRAILAFQAAGAVAFDYGNNLRAQAQEAGVTNAFD
                     YPGFVPAFIRPQFCEGRGPFRWAALSGDPEDILRTDQAVLELFPDDAGLRRWIEMAQA
                     KVPFQGLPARICWLGYGERARAGIEFNRLVAAGEVLAPIVIGRDHLDAGSVASPNRET
                     EAMADGSDAVADWPLLNALVNTAAGATWVSIHHGGGVGIGYSQHAGMVVVADGTDLAA
                     RKLERVLTTDPGMGVARHVDAGYQRAVDVARERGLRIPMLEAD"
BASE COUNT          304 a          852 c          844 g          353 t
ORIGIN      
        1 acgggcccaa gccgtcgatg cgcaaggaga tcgccatcgg cctgcgctac gtgaccgggc
       61 accgctggct gcggtcgatc gcggcgacga cggggacagc gaacttcttc ggcaacgtca
      121 tgggcgcgat cctgatcctc tacgtcaccc gcgagggcgg gctgacggcc gaggcgatcg
      181 gcttcgcgtt ctcgatcggg tcggtcggcg tcctcctggc cgcgctggtc acgagccggc
      241 tgacggcgcg cctgggcgtc ggacggatgc tcgtgctgac ctcgatcggg ttcagcctct
      301 cgggcctcct cgtcgccgcg gcctcgacga cgtatctgtt cttcgcgctc gcgctgttcg
      361 ggctgctggg tggcttcgcc agcgtcgcct ggaacatcaa ccaggtgtcg ctgcgccagg
      421 cgatcactcc gccccggatg cagggccgga tgaacgcgac gatgcgcttc atcgtgtggg
      481 gaacgatccc cttgggcgcg atcatcggcg gcatcctcgg cgacctgatc ggcctccacg
      541 cgaccatctg ggtgggcgcc atcgggggca tgttcgcctt cctgccggtc gcgacatcct
      601 cggtccgcca gatcgtcagg atgccggagc cggtggacga cgactcgtca gccgggtcca
      661 tggccgacgc ggagcctgcc taggcgtcag ccgcgcctca gtcggcctcg agcatcggta
      721 tccggaggcc gcgctcgcgg gcaacgtcga ccgcgcgctg gtagcccgcg tcgacgtgcc
      781 gcgcgacgcc catgccggga tcggtcgtga gcacccgctc gagcttgcgc gccgcgaggt
      841 ccgtgccgtc ggcgacgacc accatgcccg cgtgctggct gtagccgatc ccgacgccgc
      901 cgccgtggtg gatcgacacc caggttgcgc ccgcggcggt gttgacgagc gcgttgagca
      961 gcggccagtc ggcgaccgcg tccgagccgt cggccatcgc ctcggtctcg cggttgggtg
     1021 acgcgaccga gcccgcgtcg aggtggtcgc ggccgatgac gatgggggcg agcacctcgc
     1081 ccgcggcgac gaggcggttg aactcgatcc ccgccctggc gcgttccccg taccccagcc
     1141 agcagatgcg cgccggcagg ccctggaacg gcacctttgc ctgggccatc tcgatccacc
     1201 gccgcagacc ggcgtcgtcg gggaacagct ccaggacggc ctggtccgtg cgcaggatgt
     1261 cctcggggtc gcccgagagg gccgcccagc ggaacgggcc acggccctcg cagaactgcg
     1321 gccggatgaa ggcgggcacg aagcccgggt agtcgaacgc gttggtgacg cccgcctcct
     1381 gcgcctgcgc acgcaggttg ttgccgtagt caaaggccac ggcgccggcc gcctggaacg
     1441 cgaggatcgc gcgcacgtgg tcggccatgg agcgcatggc gcgggcctcg tacagctcgg
     1501 gctgggcggc gcgcagcgct gcggcgtcct cgagcgagat ctccgccggc acgtagccgg
     1561 cgagcgcgtc atgggcggac gtctggtccg tgacgacgtc gaagcgctcg ccggccgcgg
     1621 cccaggccgg ctcgatctcc gcggcgttgc ccaccagcgc gatcgagatc gcctggccat
     1681 ccgcggccgc cgagcgcgcc atcgccagcg cctcgtcggg cgagtgcgtc agccggtcga
     1741 catagccgat ctcgtgccgc cgtcgggcgc gcgtctcgtc gacctcgatc gccaggcaca
     1801 cgccgtcgtt catggtcacg gccagcggct gcgcgccgcc catcccgccg aggccggcgg
     1861 tgagcgtgac ccggcctcgc agcgtgccgt cgaagtgctg gcgggcgagc tcggcgaacg
     1921 tctcgaacgt gccctggagg attccctggg tgccgatgta gatccacgag cccgcggtca
     1981 tctgcccgta catcatcagg ccctgtcgct cgagctcccg gaagtgctcc caggtggccc
     2041 agcggccgac caggttcgag ttcgcgatca ggactcgtgg cgcccactcg tgggtgcgga
     2101 acacggccac gggcttgccg gactggacga gcagggtctc gtcgtccgcc agccggcgca
     2161 gctcgcggac gatggcgtcg aacgcctccc agctccgggc cgcccggccc gtgcccccgt
     2221 agaccacgag cgcgtccggg ttctcggcga cctcgggatc gaggttgttc atcagcatcc
     2281 gcagcaccgc ttcctgctgc cagccgtgcg tcgacagctc ggttccacgg ggcgcgcgga
     2341 cgggacgcgg acc
//
LOCUS       DBNHXO010000497         2129 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1945547, whole genome shotgun sequence.
ACCESSION   DBNHXO010000497 DBNHXO010000000
VERSION     DBNHXO010000497.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1945547"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2129
                     /locus_tag="AC162A_10010"
     CDS             <1..>2129
                     /locus_tag="AC162A_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5011221.1"
                     /translation="QVLDVIDEAIEAGLLLPVGGARGRYRFSHDLVRETLYDELSPGR
                     RVRLHRRIADVLETLYGPAVNEHLAELAFHFVQATVRPDGDGADDDAPVGRKAIEYAR
                     RAGDQASAALAYEEAARLYRMTLTVMALGAMTDDRARTETLLALGDVQTRTGDLDSAR
                     TTFLEASEIARRTGDGPHLARSALGFGGRHQWARPGRDGRLIPLLQDALVLLGGQDET
                     LRARLLTRLACAWRSSPERRNDSDSLSREAIDIARRLDDPATLVYTLTGRFWATWWPE
                     NPDERQAIAREVLSIAEPLRDGERIADAHFMSYLTLSELGRIHEARSALVTLARVIEE
                     VRQPAQVWLAPVNRSALALIAGDFAEAEESVASEARSQFWITPGRDEVSAARMHRFLL
                     RREQGRVAEEEAAVRQSIADFPWYPMFRSVLVCLLLDIGREAEARSEFADLARNEFAA
                     LYRDNAWLFGMSLAAEACARLGDAASADVLLEQLAPFAGRHAIAHAEGSVGVVDRYLG
                     LLAATVGRLDEAELHLTAAIDLNDRMGARPWTAHCEHDLAGVLRRRDRPGDAGAAAAL
                     DRRALATARELGMALGTEIAPPTDPAEAAPSEVPARSAEFRREGEYWSIVFERDTLRV
                     RDSRGMRHLARLLRTPGQEVHALELAAPPSTAIDRATIVEADVAMAGSDDAGPALDAE
                     AKAAYRARLIDLREELAEAESWHDPERV"
BASE COUNT          291 a          781 c          768 g          289 t
ORIGIN      
        1 caggtgctcg acgtgatcga cgaggcgatc gaggccgggc tgttgctccc ggtcggcggg
       61 gcgcgcggtc gataccgctt ctcgcacgat ctggtgcgcg agacgctgta tgacgagctg
      121 tcgccgggca ggcgcgtgcg gcttcatcgg cgcatcgcgg acgtgctcga gacgctgtat
      181 ggcccggccg tcaacgagca cctggcggag ctcgcgttcc acttcgtgca ggcaacggtt
      241 cgcccggacg gagacggtgc cgacgacgac gccccggtcg ggcgcaaagc gatcgagtac
      301 gcccgccgag cgggcgatca ggccagcgcc gcgctcgcct acgaagaggc tgcgcgcctg
      361 taccggatga cgctcaccgt gatggccctc ggcgccatga cggacgaccg cgcccggacc
      421 gagacgctgc tcgccctcgg cgacgtccag acgcggaccg gcgacctgga ctccgcgcgg
      481 accaccttcc tcgaggcctc ggagatcgcg cgccgcacag gtgacggacc acacctggcc
      541 cgctccgccc tcggcttcgg cggccgccac cagtgggcga gaccgggccg cgacgggcgc
      601 ctgatccccc tcctccagga cgctctcgtg ctgctcggcg gacaggacga gacgctgcgg
      661 gcgcgcctcc tgacgcgcct ggcctgcgcc tggcgcagct cgcccgagcg ccggaacgac
      721 agcgactcgc tgagccgcga ggccatcgac atcgctcggc ggctcgacga tcccgcaacg
      781 ctcgtctaca cgctcaccgg ccggttctgg gcgacctggt ggccggagaa cccggacgaa
      841 cggcaggcga tcgcccgcga ggtgctctcg atcgccgagc cactccggga cggggagcgc
      901 atcgctgacg cccacttcat gtcctatctg acgctgagcg agctcggtcg catccacgag
      961 gcgaggtccg cgctggtcac ccttgcccgg gtgatcgagg aggtccgcca gccggcccag
     1021 gtctggctcg cgccagtcaa ccgctcggcg ctcgcgctga ttgcaggcga ctttgcggag
     1081 gccgaggagt cggtcgcgag cgaggcccgg tcgcagttct ggatcacgcc gggccgcgac
     1141 gaggtctccg ccgcgcgaat gcaccgcttc ctcctccgtc gcgagcaggg ccgcgtcgcc
     1201 gaggaggagg ccgctgtccg ccaatcgatc gccgacttcc cctggtatcc gatgttccgg
     1261 tcggtgctcg tgtgcctgct gctcgacatc ggccgcgagg ccgaggcacg cagcgagttc
     1321 gccgacctgg ctcgaaacga gttcgcggcg ctctaccgcg acaacgcgtg gctgttcggc
     1381 atgagcctcg cggccgaggc ctgcgcgcgc ctcggtgacg ctgcgtcggc cgacgtgctc
     1441 ctcgagcagc tggcaccctt cgccggccgc cacgccatcg cgcatgccga gggcagcgtc
     1501 ggcgtcgtcg atcgctatct ggggttgctc gcggcgaccg tcgggcggct cgacgaggcc
     1561 gagctgcacc tgaccgccgc gatcgacctc aacgaccgca tgggcgcccg tccctggacc
     1621 gcccactgcg agcatgacct ggccggcgtc ctccgtcggc gtgacaggcc gggagacgcg
     1681 ggcgcggctg cggccctcga ccgccgggcg ctcgcgacgg cccgcgagct gggcatggcc
     1741 ctggggaccg agatcgcgcc gccgaccgac ccggctgagg ccgcaccgag cgaggtcccg
     1801 gcgaggtcgg cggagttccg ccgggaaggc gagtactggt cgatcgtgtt cgagcgggac
     1861 acactccggg tccgcgactc gcgcggcatg cgccatctgg cccgcctgct acgcaccccg
     1921 gggcaagagg tccacgcgct ggagctcgcc gctccaccgt cgacggcgat tgaccgggcg
     1981 acgatcgtgg aggccgacgt cgccatggcg gggtccgacg atgccggccc cgcgctcgat
     2041 gccgaggcga aggccgccta tcgggcgcgc ctgatcgacc tgcgcgagga gctggcggaa
     2101 gcggagagct ggcacgaccc ggaacgtgt
//
LOCUS       DBNHXO010000498         2366 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4724521, whole genome shotgun sequence.
ACCESSION   DBNHXO010000498 DBNHXO010000000
VERSION     DBNHXO010000498.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4724521"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="AC162A_10015"
     CDS             complement(<1..851)
                     /locus_tag="AC162A_10015"
                     /inference="COORDINATES: protein motif:HMM:NF015083.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HIR5011222.1"
                     /translation="MRHGSESFFIQGEGLVTTEVGGRRGLDTDPVNVSETPSAGAPKL
                     LAADEAPPFRFSRVGPKGTPLAAAVIRKVARAMVVEGGGNGNIPAGYTYLGQFIDHDL
                     TMDRTTVMLGDDVRPVDMLQGRSPRLDLDSLYGNGPSDAGSEKFYAADGLHLKTGSTI
                     ATPPFGAKAGHDLPRAGTGSSKKAKRVALIADPRNDENLIVAQTHLALIHFHNRVLDK
                     LPASVPASKRFSRARKKVSLHYQWLIRHDYLPRICSASVIDDVFTNGRKLVEPAASPD
                     SVPTMPV"
     gene            complement(954..1892)
                     /locus_tag="AC162A_10020"
     CDS             complement(954..1892)
                     /locus_tag="AC162A_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase"
                     /protein_id="HIR5011223.1"
                     /translation="MATRANNLPSSDIELIALLKAMKASDTVELKMTVLSEEHHATVG
                     SLPIDPVEAQPRQVFFFDTPDLQLNRAGVVARARRIQGGRGDTVIKLRPVVPEELPEE
                     LRKSPAFNVEVDVLPGNHGICSASLKGRVTGESIRDAVHGDFPLRKLFTKDQKAFYKE
                     HAPEGIELDALRVLGPTFVMKSVFIADELGRRVVAELWLYPDGSRILELSTKCLPNEA
                     FQVAAESRAYLASRGITVEIGAQQTKTKTALEFFSAHLLAGADADPVDDALGAIGSGA
                     AGHATRPEDDEDDDVLRVPEVFVELDDEDDAVAATG"
     gene            complement(1914..>2366)
                     /locus_tag="AC162A_10025"
     CDS             complement(1914..>2366)
                     /locus_tag="AC162A_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR5011224.1"
                     /translation="RFSVEVGAVRLTEQFGLGAALSREALDRALAAIATELVRVDGHG
                     VPDVLVGMGGAVTNIAAVMHGMATYDPDRIQGSVIDRAEIERQIELYRTRDVDARREI
                     VGLQPKRADVILAGACIVRTLMDKVGRDALTVSDRGLRHGVFVERFGG"
BASE COUNT          322 a          845 c          808 g          391 t
ORIGIN      
        1 tcgacgggca tggtcggcac ggagtccggt gaggcggcgg gctcgaccag cttgcggccg
       61 ttggtgaaca cgtcgtcgat caccgacgcg ctgcagatcc ggggcaggta gtcatggcgg
      121 atcagccact gatagtggag gctgaccttc ttccgcgccc gactgaagcg cttcgaggcc
      181 gggaccgatg ccggcagctt gtccagcacc cggttgtgga agtggatcag cgcgaggtgg
      241 gtctgggcga cgatgaggtt ctcgtcgttg cgtgggtcgg cgatcagggc gacccgcttg
      301 gccttcttgc tcgacccggt cccggcgcga ggcaggtcgt gcccggcctt ggcgccgaac
      361 ggcggggtcg cgatggtcga tcccgtcttg aggtggagcc cgtcggccgc gtagaacttc
      421 tcggaccccg cgtcactcgg gccgttgccg tacagcgagt cgaggtccag tcgcggcgac
      481 cggccctgca gcatgtcgac cggacggacg tcgtccccga gcatcacggt cgtccggtcc
      541 atggtcaggt cgtggtcgat gaactggccg aggtaggtgt acccggccgg gatgttcccg
      601 ttgccgccgc cctccaccac catcgcgcgg gcgaccttgc ggatcacggc cgctgccagc
      661 ggggttccct tggggccgac ccgcgagaac cggaacggcg gtgcctcgtc ggcggcgagc
      721 agcttcggag cgcccgcgct gggcgtctcg gacacgttga ccgggtcggt gtccaagccc
      781 ctgcgcccgc cgacctcggt ggtgaccaga ccctcgccct ggatgaagaa gctctcggac
      841 ccgtggcgca tggcgatcca ccccctggcg gcgccctggt cgacaacgtc aggccacagg
      901 gcgctccgtg gtcgcattgt ctgccacctg tcgatccctc cggccgacgc cggctagccc
      961 gtagctgcga ccgcgtcgtc ctcgtcgtcc agctcgacga acacctcggg cacccgcagc
     1021 acgtcgtcgt cctcgtcgtc ctctggcctg gtcgcgtggc ccgccgcgcc ggagccgatg
     1081 gcgcccaggg cgtcgtcgac ggggtccgcg tcggccccgg cgagcaggtg cgcgctgaag
     1141 aactcgagcg ccgtcttggt cttggtctgc tgcgcgccga tctcgaccgt gatgccgcgc
     1201 gaggcgagat aggcgcggct ctcggcggcc acctggaacg cctcgttggg caggcacttc
     1261 gtcgacagct cgaggatccg tgagccgtcc gggtacagcc acagctcggc gaccacgcgg
     1321 cggcccagct cgtcggcgat gaacaccgac ttcatgacga aggtcggccc caggacccgc
     1381 agcgcgtcga gctcgatgcc ctcgggcgcg tgctccttgt agaacgcctt ctggtccttc
     1441 gtgaacagct tgcgcaaggg gaagtcgccg tgaacggcgt cgcggatcga ctcgcccgtc
     1501 acacgtccct tgagcgacgc cgagcagatc ccgtggttgc ccggcagcac gtcgacctcg
     1561 acgttgaacg cgggcgactt ccgcaactct tcgggaagct cctccgggac cacgggccgg
     1621 agcttgatga ccgtgtcccc tcgcccaccc tggatccgcc gcgcccgcgc gacgacaccc
     1681 gccctgttca gctggaggtc cggcgtgtcg aagaagaaca cctggcgcgg ctgcgcctcc
     1741 accgggtcga tgggcaggct gccgaccgtc gcgtggtgct cctcgctgag aacggtcatc
     1801 ttgagctcga cggtgtcgga ggccttcatc gccttgagca gcgcgatcag ctcgatgtcg
     1861 ctcgacggca ggttgttcgc gcgcgtcgcc atgggttctc cctccttcgg ccgtcagccg
     1921 ccgaatcgct cgacgaacac cccgtgtcgc agcccgcggt cgctcaccgt gagtgcgtca
     1981 cggccgacct tgtccatgag ggtgcgcacg atgcacgccc ccgccaggat cacgtctgcg
     2041 cgctttggct ggaggcccac gatctcccgg cgcgcgtcaa cgtcgcgggt gcggtacagc
     2101 tcgatctggc gctcgatctc ggcgcggtcg atcacgctgc cctggatacg gtccggatcg
     2161 taggtcgcca tgccatgcat caccgcggcg atgttggtca cggcgccgcc catgccgacg
     2221 agaacgtccg gaaccccgtg gccgtcgacc cggacgagct cggtcgcgat cgccgcgagc
     2281 gcccggtcta gcgcctcgcg cgacagggcc gctcccagcc cgaactgctc cgtcagcctg
     2341 accgccccga cctcgacgct gaaccg
//
LOCUS       DBNHXO010000499         1845 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1945847, whole genome shotgun sequence.
ACCESSION   DBNHXO010000499 DBNHXO010000000
VERSION     DBNHXO010000499.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1945847"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="AC162A_10030"
     CDS             complement(<1..838)
                     /locus_tag="AC162A_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012876.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR5011225.1"
                     /translation="MAAVRPPDTRWPDSRGSRRPTVPRSFQTRLTIAFIGVVAMTLLL
                     VGPVVINRIDDYFRQQEEQSLLVRATATARIVGEFLDAQLAGAGVVHVNEQSGEAELN
                     PDVSGMLDSGLLTLVADNVAEADVSLEFGTARQDTDGRFVPDPHRDLSYAAQLDIEPA
                     AGQARDPQVSPARVEYGTANENGDWGMVVTLSNPYTSRASTLAAVTGLLLLMAGIALM
                     VAVLVAAFLARRFTTPLTRLTEASRRLAEGDLSSRVPTDEVSSSTTELRALSQQFNTM
                     ANR"
     gene            complement(840..1535)
                     /locus_tag="AC162A_10035"
     CDS             complement(840..1535)
                     /locus_tag="AC162A_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5011226.1"
                     /translation="MATILIVEDEFAVARGIQYALQQEGYEVTIARSGEEGLEFATNQ
                     APDLIVLDVRLPGIDGFEVLRRLRAVGSKAPVLFLTARGDEFDKVVGLELGADDYVTK
                     PFALRELMSRIRALLRRAYGDLANASGGRVIRHGDLVIDLEKRRVQRGSRRIGLTATE
                     FEILRHLASRPGRVFSRRELLELLRDYEALDQDEKTINVHVSHLRDKIEDDPSTPQFV
                     LTVRGAGYQFAEH"
     gene            1724..>1845
                     /locus_tag="AC162A_10040"
     CDS             1724..>1845
                     /locus_tag="AC162A_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HIR5011227.1"
                     /translation="MPDRPRLAAASRTVTGKAVAHMRKAGRVPAVVYGHGTASEP"
BASE COUNT          253 a          646 c          679 g          267 t
ORIGIN      
        1 gccggtttgc catcgtgttg aactgctggg acagggcccg gagctcggtc gtgctcgagg
       61 acacctcgtc ggtcgggacg cgcgaggaca ggtcgccctc ggccagccgg cgcgaggcct
      121 ccgtgagacg ggtgagcggc gtcgtgaacc ggcgggcgag gaacgcagcc acgaggacgg
      181 cgaccatgag ggcgatcccg gccatcagca gcagcaggcc cgtgacggcc gccagggtgc
      241 tcgcccggct cgtgtacggg ttcgacagcg tgacgaccat gccccagtcg ccgttctcgt
      301 tcgccgtccc gtactcgacg cgcgcgggtg acacctgcgg gtctcgagcc tgacccgccg
      361 ccggctcgat atccagctgt gcggcgtagc tcaggtctct gtgcgggtcc gggacgaagc
      421 gcccatccgt gtcctggcgt gcggtgccga actcgaggga cacgtcggcc tcggcgacgt
      481 tgtccgccac cagcgtcagc agtccgctgt cgagcatgcc gctcacatcc gggttgagct
      541 cggcctcccc gctctgctcg ttcacgtgga cgacgccggc tcccgcgagc tgggcatcga
      601 ggaactcccc gacgatccgt gcggtcgccg tggcccgcac gagaaggctc tgctcctcct
      661 gctggcggaa gtagtcgtcg atccggttga tgacgaccgg cccgacgagc agcagcgtca
      721 tcgcgacgac gccgatgaag gcgatggtga gccgcgtctg gaacgagcgg gggacggtcg
      781 gccggcgcga gccgcggctg tcgggccagc gggtgtccgg gggccggacg gcggccacgt
      841 cagtgctcgg cgaactggta gccggcgccc ctgacggtca ggacgaactg cggcgtggac
      901 gggtcgtcct cgatcttgtc gcgcaggtgg ctcacgtgga cgttgatggt cttctcgtcc
      961 tggtcgaggg cctcgtagtc gcgcagcagc tcgagcagct cgcggcggct gaacacccgg
     1021 cccggccggc tcgcgaggtg gcggaggatc tcgaactcgg tcgccgtgag gccgatccgc
     1081 cggctgcccc gctggacgcg gcgcttctca aggtcgatca cgaggtcccc gtgtcggatg
     1141 acgcggccgc cggacgcgtt ggccaggtcg ccgtaggccc ggcgcagcag cgcgcggatc
     1201 cggctcatca gctcgcgcag cgcgaacggc ttggtgacgt agtcgtcggc gccgagctcg
     1261 aggccgacga ccttgtcgaa ctcgtcgccg cgggcggtca ggaacaggac cggcgccttc
     1321 gatcccaccg cgcggaggcg gcgcaggacc tcgaacccat cgatccccgg caggcgcacg
     1381 tcgagcacga tgaggtccgg ggcctggttg gtcgcgaact cgagcccctc ctcgccggag
     1441 cgcgcgatcg tcacctcgta cccctcctgc tggagggcgt actggattcc tcgggcgacg
     1501 gcgaactcat cttcgacgat caggatggtg gccacggcgc ggcgatccta caccgggcgg
     1561 cctcgatggt tccccggcgt cgcccgtggg ggtgcgggcg cgcgtgtgat agcctccggg
     1621 gcgatgcagc gacggcggga tgccagtcgc gccgcggcgc cgcctgcggg aacgatccgc
     1681 cacctgcggg tgcgagcgct gccaccagaa gaggttcacc acgatgcctg accgcccccg
     1741 cctcgcggcc gccagccgga ccgtgaccgg caaggccgtc gcccacatgc gcaaggcagg
     1801 ccgcgtgccg gccgtcgtct acggccacgg cacggccagc gagcc
//
LOCUS       DBNHXO010000500         4000 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3335520, whole genome shotgun sequence.
ACCESSION   DBNHXO010000500 DBNHXO010000000
VERSION     DBNHXO010000500.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3335520"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..952
                     /locus_tag="AC162A_10045"
     CDS             182..952
                     /locus_tag="AC162A_10045"
                     /inference="COORDINATES: protein motif:HMM:NF018299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011228.1"
                     /translation="MATGLDAMEASSSPAFVVVGVFDGLHRGHAYLLEHLVAEAARRS
                     ARPVVITFDHHPDEVLVGNAPPILCDPQERLERLDAAGVETTVVVHFDRALRETPYEV
                     FVDRILARTSLAGFLMTPDAAFGYQRAGTPASLERLGRSRGFEVVVVPAFDVDGRAVS
                     STLVRAAIGAGDLDGAARMLGRPHAVVGEAGEAGEGSVLRYPLPVALPPQGTYRVRLA
                     SGSSDQEEADGVAVIDDGLTLPEHRGVGRTRVAFIARR"
     gene            1040..2398
                     /gene="xylA"
                     /locus_tag="AC162A_10050"
     CDS             1040..2398
                     /gene="xylA"
                     /locus_tag="AC162A_10050"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HIR5011229.1"
                     /translation="MPRVGESFFTDVEGPIPFGGLDSDDPLAFKVYQPDRVVLGKRME
                     DHLRAGVCFWHSFAWPGMDMFGVGTLDRPWLAPDADPMDAARTKMAVAFEFFTKLGTP
                     YYCFHDRDVAPETGGFAEFRSNLDALVDDAEGYQERTGVRLLWGTANLFTHPRYQAGA
                     ATNPDPEAFAYAAAQVKHMLEVTKRLGGQNYVLWGGREGYDTLLNTDLGREGAQLARF
                     LHLVVEHKHKIGFEGQLLIEPKPMEPTKHQYDYDTATVHGFLVRNGLEGEYRANIEAN
                     HATLAGHSFHHEVAYATANGMLGSIDANRGDPQNGWDTDQFPNSVEDLTLALYEILRA
                     GGIAPGGFNFDAKLRRQSSARSDLFHAHIGGLDTLARALLAAADLVERGELAALKDAR
                     YAGWDGDLGKAILGGSLSLEALEARVADGSIDPRRASGAQERLENVVNQAIWAVDRPR
                     RA"
     gene            2402..3886
                     /gene="xylB"
                     /locus_tag="AC162A_10055"
     CDS             2402..3886
                     /gene="xylB"
                     /locus_tag="AC162A_10055"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR5011230.1"
                     /translation="MGVVLGIDVSTTATKAVVLDSAGAVLAIGTAAYGFEQPHPLWSE
                     QDPALWWDGAVAAIRGALGVAGVAASDIEGIGLTGQMHGLVVLDAAGEVLRPSILWND
                     QRTAAECDLIRAWVGPERLVRITGNDALTGFTAPKLAWVRAHEPDVWARVAHVLLPKD
                     YLRYRLTGEYAMDKADGAGTQLFDLAARDWSPVMLDALGIDPGWMPPTFEGPEITGTL
                     TAAAADATGLRAGTPVMAGGGDQAANAVGVGAVVPGRVALSLGTSGVVFATTDAPLFE
                     PAGRVHAFCHAVPGKWHMMSVMLSAAGSLRWFRDAIAPGVEFGELVDAAAEVPAGSDG
                     LLFLPYLSGERSPHPDPLARGAFVGLTLAHDRRHLTRAVLEGVAFGLRDGLDLMTGAG
                     MPAPAQIRASGGGTASPLWRQILADVLQAEIATVSTTEGAACGAGLLAAVGAGWFPTV
                     EAAADAVVVATPTAAPGPDAPRYAAMHTRYRELYPALAPSFRRL"
BASE COUNT          530 a         1450 c         1461 g          559 t
ORIGIN      
        1 gatgcgccga tcgtcctcgc cgtggggcct gacggccgcg tcgccgccgt ctgccgtgcc
       61 gtcgccggtg ccctgcaccc gcacaaggtg ctggtcgagc gcgacatgac gccgcgcgat
      121 cgcggcgtcg agggcgatgc cgcggtgctc gggaccgcgg ccgaggtcgg ctgagatgcg
      181 ggtggcgacc ggtctcgacg cgatggaggc gtcgtcgagc ccggccttcg tggtcgtggg
      241 cgtgttcgac ggcctgcatc gaggccatgc ctacctgctc gagcacctcg tcgccgaggc
      301 cgcgcgccgg tcggccaggc cggtcgtgat cacgttcgac catcatccgg acgaggtgct
      361 cgtgggcaac gccccgccga tcctgtgcga cccgcaggag cgcctggagc ggctcgacgc
      421 ggccggggtc gagacgacgg tcgtcgtcca cttcgaccgg gcgctgcgcg agacgccgta
      481 cgaggtattc gtcgaccgga tcctggcccg cacgagcctc gcgggcttcc tcatgacgcc
      541 cgacgcggcc ttcggctacc agcgcgcagg gacgcccgcc tccctcgagc ggctcggccg
      601 ctcgcgcggc ttcgaggttg tcgtggttcc cgcattcgac gtcgacgggc gcgcagtcag
      661 cagcaccctg gtccgggcgg ccatcggcgc gggggacctc gacggggccg cccgcatgct
      721 cgggcgcccg cacgccgtgg tcggcgaggc gggcgaggcg ggggagggga gcgtgctccg
      781 gtacccgctc cccgtcgccc tgccccccca agggacatac cgggtccggc tcgcctcggg
      841 ttcatccgac caggaggagg ccgatggcgt ggccgtgatc gacgacgggc tgacgcttcc
      901 cgagcatcgc ggcgtgggcc ggacgagggt ggcattcatc gcccgtcgct gaacgtcgcc
      961 cggtaggatt gtgcaagcgc ctcacaaacc ggtggggcgg ccaccgcacg tggacgcgcg
     1021 gcggccggaa cgcaggagga tgccgagggt gggcgagtcg ttcttcaccg acgtcgaggg
     1081 gccgatcccg ttcggcgggc tcgacagcga cgatcccctg gcgttcaagg tctaccagcc
     1141 cgaccgcgtg gtcctgggca agcgcatgga ggaccacctt cgcgcaggcg tgtgcttctg
     1201 gcactcgttc gcgtggcccg gcatggacat gttcggggtc gggacgctcg atcggccatg
     1261 gctcgccccg gacgcggacc cgatggacgc cgcccggacg aagatggccg tcgcgttcga
     1321 gttcttcacg aagctcggca cgccgtacta ctgcttccac gaccgcgacg tcgcccccga
     1381 gaccggtgga ttcgccgagt tccgctcgaa cctcgacgcc ctcgtcgacg acgccgaggg
     1441 ctatcaggag cggaccggcg tccggctgct gtggggcacc gccaacctgt tcacccaccc
     1501 gcgctatcag gcgggcgccg cgaccaaccc tgacccggag gcgttcgcgt acgccgcagc
     1561 gcaggtcaag cacatgctcg aggtcaccaa gcggctcggc ggccagaact acgtgctgtg
     1621 gggtggccgc gagggctacg acaccctgct caacaccgac ctggggcgcg agggcgcgca
     1681 gcttgcccgc ttcctccacc tggtcgtcga gcacaagcac aaaatcggct tcgagggcca
     1741 gctgctgatc gagcccaagc cgatggagcc gaccaagcac cagtacgact acgacaccgc
     1801 gaccgtgcac ggcttcctcg tgcgcaacgg gctcgagggc gagtaccggg ccaacatcga
     1861 ggccaaccac gcgacgctcg cgggccacag cttccaccac gaggtcgcgt acgcgaccgc
     1921 caacgggatg ctgggcagca tcgacgccaa ccgcggcgat ccgcagaacg gctgggacac
     1981 cgaccagttc ccgaattccg tcgaagacct cacactggcg ctgtacgaga tcctgcgcgc
     2041 gggcgggatc gcgccgggcg ggttcaactt cgacgccaag ctgcgccgcc agagcagcgc
     2101 tcgctcggac ctgttccacg cccacatcgg cggcctcgat accctcgcac gtgcgctgct
     2161 ggcggccgcc gacctggtcg agcgcggcga gctggcggcg ctgaaggacg cgcggtacgc
     2221 cggctgggac ggtgacctcg gcaaggccat cctcgggggc tcgctctcgc tcgaggccct
     2281 cgaagcgcgc gtcgccgacg ggtccatcga tccgcggcgg gcatccgggg cccaggagcg
     2341 gctcgagaac gtcgtcaacc aggcgatctg ggcggtcgac cgaccccggc gggcctgacc
     2401 gatgggcgtc gtcctcggga tcgacgtctc gaccacggcg accaaggcgg tcgtcctcga
     2461 ttcagcaggc gccgtgctcg cgatcgggac ggcggcgtac gggttcgagc agccgcaccc
     2521 cctgtggagc gagcaggacc cggcgctgtg gtgggacggg gccgtcgcgg cgatccgcgg
     2581 ggcgctgggc gtggccggcg tcgcggcgtc cgacatcgag gggatcggcc tcaccggcca
     2641 gatgcatggc ctcgtcgtgc tggatgcggc gggcgaggtg ctgcgcccgt cgatcctgtg
     2701 gaacgatcag cgcaccgcgg ccgagtgcga cctgatccgg gcatgggtag gcccggagcg
     2761 cctcgtccgg atcacgggca acgacgcgct gaccgggttc acggcgccca agctcgcctg
     2821 ggtgcgggct cacgagcccg acgtctgggc gcgggtcgcc cacgtgctgc tgcccaagga
     2881 ctacctgcgc taccgcctga ccggcgagta cgccatggac aaggcggatg gcgccgggac
     2941 ccagctgttc gacctcgcgg cccgcgactg gtcgccggtg atgctggacg cgctcggcat
     3001 cgacccgggc tggatgccgc cgacgttcga gggcccggag atcaccggca cgctgacggc
     3061 cgccgcggcc gacgcgaccg ggctccgcgc cgggacaccg gtcatggcgg gtggcggtga
     3121 ccaggcggcc aacgcggtcg gcgtgggcgc cgtcgtcccc ggacgcgtgg cgctctcgct
     3181 ggggacgtcc ggtgtcgtgt tcgcgaccac cgacgcgccg ctgttcgagc cggcgggccg
     3241 cgtccacgcg ttctgccacg ccgtgcccgg caagtggcac atgatgtcgg tcatgctctc
     3301 ggccgccggg agcctgcgct ggttccggga cgcgatcgcg ccgggcgtcg agttcggcga
     3361 gctggtcgac gcggccgccg aggtgcccgc tggcagcgac ggcctgctgt tcctgcccta
     3421 cctctcgggc gaacgcagcc cgcaccccga cccgctcgcc cgcggcgcct tcgtggggtt
     3481 gaccctggcc cacgaccgga ggcacctcac gcgcgcggtg ctcgagggcg ttgcgttcgg
     3541 cctgcgtgac ggcctggacc tcatgaccgg cgccggcatg cccgcccctg cgcagatccg
     3601 ggcgtccggc ggcggcaccg ccagcccgct ctggcgccag atcctcgcgg acgtcctgca
     3661 ggcggagatc gcaacggtca gcacgaccga gggcgcagcg tgcggcgcgg ggctgctcgc
     3721 ggccgttggt gcgggttggt tcccgaccgt cgaggcggcg gcggatgccg tggtcgtcgc
     3781 gacacccacc gcggcgcccg ggccggacgc gccgcgctac gcggccatgc acacccggta
     3841 tcgcgagctc taccccgcgc tcgcgccctc gttccggcgg ctgtagcggg tcggagcccg
     3901 cgcgccgcta cccgacgagc tcggccagct ccgtcttgga cacctcgtcc ggcttcttgc
     3961 cgatgatgat catcgcgagg acctcgtcct tggtgacgtc
//
LOCUS       DBNHXO010000501         7442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4725250, whole genome shotgun sequence.
ACCESSION   DBNHXO010000501 DBNHXO010000000
VERSION     DBNHXO010000501.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4725250"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="AC162A_10060"
     CDS             <1..342
                     /locus_tag="AC162A_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011231.1"
                     /translation="GWSPTEGPAAMMGIALAAVLTIALLGWAALFATIGRGYIAPLAW
                     TVAMVAASQILTVLGWGAWFPWAVPALVAGAGGTAVEPVALGGVFVLLVAAAVGLVAT
                     VAWWDRADQTG"
     gene            410..1177
                     /locus_tag="AC162A_10065"
     CDS             410..1177
                     /locus_tag="AC162A_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5011232.1"
                     /translation="MDLDLNGRRALVGGGGSGIGSGIATALAAEGARVALIGRTGARL
                     EAEAARLGGTAVIADLATPDGPAGAVEAAVAALGGIDLLVVNTGGPPGGSFDTLGEAE
                     WEQAIAGTLQSTLRLIRAALPHLREGQDPSILIVLSSSVREPIPNLTTSNVLRPGLAG
                     LVKTLTAELAPIRINGLAPGRIATDRIAYLDGRRAEATGVSVEEIGERTRATIPLGRY
                     GDVAEIGRVGAFLLSPAASFVTGAIVPVDGGMIRALP"
     gene            complement(1060..2103)
                     /locus_tag="AC162A_10070"
     CDS             complement(1060..2103)
                     /locus_tag="AC162A_10070"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5011233.1"
                     /translation="MTPSPAPSPARSNSFADLGSLLFLGAVWGGVWLFMRVAAPEVGP
                     VWAAEVRVALAALILVVVAGRRTWRTARGRIGDFLFTGATFSAIPFTLIAFATLTLPV
                     GLGALLNAATPLFTALVGAFVLGQRLSGRVIAGVAVGMVAVAVVVGWSPLDLGPGTLA
                     AVGAALGAALSYAVAGTFVRRRLHGIPGIEVATGQLVSASIVLLPFAIASGAPGVPSA
                     AGVASLVAVAVISTALAWPLYFRVLGRTSATAASTVTFIVPAFAIVWGAIALDESIGP
                     EVVVGFALIMVSLALVLGLPLPGGLGRVPARVPQGRARIMPPSTGTIAPVTNDAAGDR
                     RKAPTRPISATSP"
     gene            complement(2165..3097)
                     /locus_tag="AC162A_10075"
     CDS             complement(2165..3097)
                     /locus_tag="AC162A_10075"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR5011234.1"
                     /translation="MGDIEALRGVWNIVPTPFLPDGELDVASIPRLVSFVRATGVDGL
                     TILGVLGEAARLGDEERTTVLRTALEAAAGSIPICVGVSHASTDRAIVYAREAEAAGA
                     HSVMLAPPPLARPTDAALRRHYLAVASAISIPVVVQDHPASSGVPMSVDFLLALAVEA
                     PSCRVIKLEEEPTPPKAGRLVDGSPDVIVLGGLGGEMLIEELRRGSHGTMTGFGFPEI
                     LVAVVRRWFAGDRDGAAALFDRYATVVRFENQAILNLAIRKVVYQRRGAIAHATLRAP
                     GMPPDAGTLADLDDLLRRTGLHQHLEAGAFTGVD"
     gene            complement(3300..4589)
                     /locus_tag="AC162A_10080"
     CDS             complement(3300..4589)
                     /locus_tag="AC162A_10080"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5011235.1"
                     /translation="MTAALTTVRPLRLTVVATGALVAGAIVLRLANPYHLPGPSVSAV
                     VPLLWAAGALVVLLLTQAWAPTLGWIAAIVGSTAAALAIVGNDWEARRLVDMGDDRWL
                     GLLVVAALLIPAVIAAAYAALGLGRAVVIAAWTAVGVLTVGLVSRYVARTLGGGDLDG
                     TLPEWAWLLLIGLLAAIGLTRDLRPALSRTRVRLAAGDAGGSGRGGPVAALPALRVFM
                     DELVPGRDAGRKEAVETERGRLAADLHAEVLPSLRRALAAAEAGGTVERLASDLRIAV
                     DEVESMLLARRSIVLEEMGLLAGLEWLAERVEDRSDVRVEITVEGGGEAGATDRPPRE
                     AERAAFRVAQLALDNVVRHAPSSTAGVSVAVGPRVIRLRVEDDADSAPIDEAAAARSG
                     RRGIADMRAEATACGGALEVWPGAGDRGTAIVFRWPA"
     gene            complement(4586..5218)
                     /locus_tag="AC162A_10085"
     CDS             complement(4586..5218)
                     /locus_tag="AC162A_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5011236.1"
                     /translation="MTGQPIRVVLVDDHPAILDAVGSAVRSAPDLILAGSARSLDDAR
                     ALLAVTTGIDVVVTDVQLDGEAEGLRLLEAVGPDGPSVLLLSSFDQPPLVRTAFERGA
                     AGYLLKTSEVPEILDAIRTVAGGGTAFSASMLRAIRSAPRRPSDREIEVLALVCGGAS
                     NDEIGARLGVSEKTVESHLRRLFDRYGVLSRTELAVLALREDWVPDKSRP"
     gene            5345..6445
                     /locus_tag="AC162A_10090"
     CDS             5345..6445
                     /locus_tag="AC162A_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011237.1"
                     /translation="MNEDEATPGGDATPGSDAPAGAPPPPPAYPVAPAYPPSPQYPAW
                     IAPVSAHTGPRIDVGSVLGRTFDTFGREWSLFLVLAIPAAVASALRLVITPTFESQVQ
                     NRFAATTNIGDPWALFVVSVIVATAVGFSALASVVAADGLWRGQPAGIVDTASTVVRS
                     IPRAIPVWLLIVLAQAAIVVFTTQLTPQTPVTRQSLAASSGAAALVLLAIPLVIVGAI
                     VAVVIQARLSLVLPVIALEEGSPGSVIPRTWRLTKGNVIMLFGCSLLIGLCVSVTAWG
                     STLFLLFGANRLIAGVALAIAELVTAPLTGIWVAIAWGDLVGGRHADNELMARGRGRW
                     TTATIVFGLGALLVVAGLGITGAAVTRLVDAP"
     gene            6805..6948
                     /locus_tag="AC162A_10095"
     CDS             6805..6948
                     /locus_tag="AC162A_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011238.1"
                     /translation="MDDRTQPGPDQGRKFPEPGPDQGKKFPGPDSGLYRREAGPERGF
                     YRR"
     gene            6977..7153
                     /locus_tag="AC162A_10100"
     CDS             6977..7153
                     /locus_tag="AC162A_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011239.1"
                     /translation="MDDRAQPGPEQGFKIPGPVRGFYRHFTGPDGGWYRRFSPDGGIF
                     RREAGPDRGMYRGH"
BASE COUNT         1071 a         2714 c         2755 g          902 t
ORIGIN      
        1 gggtggtcac cgaccgaggg gcccgcggcg atgatgggca tcgcgttggc agcggtcctc
       61 acgatcgcgc tgctcggatg ggccgcgctg ttcgccacca tcggacgcgg ctacatcgca
      121 ccgctcgcct ggaccgtggc gatggttgcc gcatcgcaga tcctcaccgt cctcggctgg
      181 ggcgcgtggt ttccatgggc ggtgccggca ttggtcgccg gcgccggcgg cactgcggtc
      241 gagccggtcg ccctgggagg tgtcttcgtg ctcctcgtgg ccgcggccgt gggacttgtg
      301 gcgacggtcg cctggtggga ccgcgccgac cagaccggct gaggcgttgc gagcctgtcg
      361 gctggcgtac cggcccgggt acgctgatag gcacgcattg gaggcgctga tggatctcga
      421 cctgaacggg cgacgggcac tggtcggtgg cggcggatcg gggatcggca gcggcatcgc
      481 cacggcgctc gcggccgagg gtgcccgggt ggccctgatc ggccggaccg gtgcccggct
      541 cgaagccgag gcggcgcggc tcggcgggac cgcggtcatc gcggatctgg cgacgcccga
      601 cggcccggcg ggtgccgtcg aagcggcagt cgcggccctg ggcggcatcg acctgctcgt
      661 cgtcaacacc ggcggtccgc cgggcggctc cttcgacacg ctcggcgagg ccgagtggga
      721 acaggcgatc gccggcaccc tccagtcgac gctgcgcctg atccgcgcgg ccctcccgca
      781 cctgcgcgag ggtcaggatc cgtcgatcct gatcgtgctc tcgtcgtccg tgcgcgagcc
      841 gatcccgaac ctcacgacgt cgaacgtcct gcgtccgggg ctcgccggcc tcgtcaagac
      901 gctcaccgcc gagctcgcgc cgatccggat caacgggctg gcgcccggcc ggatcgcgac
      961 cgaccggatt gcctatctcg acgggcgccg agccgaggcg acgggcgttt cggtcgagga
     1021 gatcggggaa cggacccggg cgacgatccc gctgggccgc tacggggacg tcgcggaaat
     1081 cgggcgggtc ggagccttcc tcctgtcgcc cgcggcgtcg ttcgtgaccg gcgcgatcgt
     1141 ccccgtcgac ggaggcatga tccgcgccct gccctgaggc acccgggccg ggactcggcc
     1201 gaggccaccc ggcagcggca gcccgaggac cagcgccagg ctgaccatga tgagcgcgaa
     1261 gcccacgacg acctcggggc cgatcgactc gtccagggcg atcgcgcccc acacgatggc
     1321 gaacgccggc acgatgaacg tgaccgtgct cgccgccgtg gcggaggtgc ggcccaggac
     1381 gcggaagtag agcggccagg ccagggcggt ggagatgacc gcgacggcga cgagcgaggc
     1441 gacccccgcg gcggacggca cgccgggcgc ccccgaggcg atcgcgaacg gcagcagcac
     1501 gatcgacgcc gagacgagct gacccgtggc gacctcgatg ccggggatac cgtgcagccg
     1561 gcggcggacg aaggtgccgg cgacggcgta gctgagggcg gcgcccaggg cggcgccgac
     1621 ggccgcgagg gtgccggggc cgagatcgag gggcgaccag ccgacgacca cggcgacggc
     1681 gaccatcccg acggccaccc cggcgatgac gcggcccgag aggcgctggc ccaggacgaa
     1741 cgcgccgacg agcgcggtga acagcggcgt ggcggcgttc agcagggcgc cgagaccgac
     1801 cggcagcgtc agcgtcgcga acgcgatgag ggtgaacggg atcgccgaga acgtggcgcc
     1861 cgtgaacagg aagtcgccga tccggccgcg tgccgtgcgc cacgtccggc ggcccgccac
     1921 cacgacgagg atcagcgcgg ccagtgcgac ccggacctcg gccgcccaga cgggaccgac
     1981 ctcgggggcc gccactcgca tgaacagcca gaccccgccc cagacggcgc ccaggaacag
     2041 cagcgagccg agatcggcga aggagttgga tcgggcgggc gagggagcgg gcgagggcgt
     2101 catccccaga gtgtcgagcg cgccccgcag cccgctagtt gacggccgtc gagatcggcc
     2161 tacgtcaatc gacgcccgtg aacgcccccg cttcgaggtg ctggtggagc ccggtgcgcc
     2221 gcaggaggtc gtcgaggtcg gcgagggtcc cggcgtcggg cggcatgccg ggtgcgcgga
     2281 gcgtggcgtg ggcgatcgcg ccgcgccgct ggtagacgac cttgcggatc gcgaggttca
     2341 ggatcgcctg gttctcgaag cggacgaccg tcgcgtacct gtcgaacagc gcggcggcgc
     2401 cgtcgcgatc gccggcgaac cagcgccgga cgacggccac caggatttcc gggaacccga
     2461 acccggtcat cgtcccgtgg ctgccgcgac gcagctcctc gatcagcatc tccccgccga
     2521 ggccgccgag cacgatgacg tcgggcgagc cgtcgaccag gcggccggcc ttgggcggcg
     2581 tcggctcctc ctccagcttg atcacgcggc acgacggcgc ctcgaccgcc agggcgagca
     2641 ggaagtcgac gctcatgggg acgcccgagc tcgcggggtg gtcctggacg acgacgggga
     2701 tcgagatcgc cgaggccacg gcgaggtagt ggcgacggag ggcggcgtcg gtcgggcggg
     2761 cgaggggcgg tggggcgagc atgaccgagt gcgcgccggc ggcctcggcc tcgcgggcat
     2821 acacgatcgc gcggtccgtc gaggcgtgcg acacgccgac acagatcggg atcgacccgg
     2881 cggcggcttc gagggcggtc cggagcacgg tggtgcgctc ctcgtcgccc agcctcgcgg
     2941 cctcgccgag cacgcccagg atcgtcaggc cgtcgacgcc cgtggcccgc acgaaggaca
     3001 cgagccgcgg gatcgacgca acgtccaact cgccgtccgg caggaacggc gtcggcacga
     3061 tgttccagac cccgcgcagc gcctcgatgt cgcccatgcc gcctcctgtc gtgcgtggat
     3121 cgagcctaca ggagcgggcg gcccgcgcag gctggcctca ggggccagcg aatcgcgcac
     3181 tggggcggat cagccggccg ctggacgccg cttctggcct gccaggccgg atccgcacct
     3241 caggcgaggc cggtcggctg gttcacgccg attcgctggc ctgccaggca agcgaccgtc
     3301 tacgcgggcc agcggaagac gatcgccgtg ccgcgatcgc cggccccggg ccacacctcg
     3361 agcgcgccgc cgcacgctgt cgcctccgcg cgcatgtccg cgatgccgcg ccgcccggag
     3421 cgcgccgccg ccgcttcatc gattggtgcc gagtcggcgt cgtcctccac ccgtaggcgg
     3481 atgacccgcg ggccgacggc gacggagacc cccgccgtcg acgatggcgc gtgacgcacg
     3541 acattgtcga gcgccagctg cgcgacccgg aacgccgccc gctccgcctc gcgcggcggc
     3601 ctgtccgtag ccccggcctc cccaccgccc tcgaccgtga tctcgacccg aacgtcggac
     3661 cggtcctcca cgcgctcggc cagccactcg agcccggcca gcaggcccat ctcctcgagc
     3721 acgatcgacc gtcgcgccag cagcatcgac tcgacctcgt cgacggcgat gcgcaggtcg
     3781 ctcgccagcc gctccaccgt cccgccggcc tcggccgcgg caagggcgcg acgcagcgac
     3841 ggcagcacct cggcgtggag atcggcggcc agccggccac gctccgtctc gaccgcctcc
     3901 ttgcggcccg cgtcccggcc cggcacgagc tcgtccatga acacgcgcag cgccggcagc
     3961 gcggcgaccg gaccgccccg tcccgaaccg cccgcatcgc cggcggccag tcgcacccgc
     4021 gtgcgcgaga gtgcggggcg caggtcgcgg gtcaggccga tcgccgccag gaggccgatc
     4081 aggagcagcc acgcccactc cgggagcgtg ccgtcgaggt cgccgccacc gagcgtccgg
     4141 gcgacgtacc gggacacgag gcccacggtg agcacgccga cggccgtcca ggcggcgatc
     4201 accacggcgc gaccgagacc gagcgccgcg tacgccgccg cgatgaccgc ggggatcaac
     4261 agcgccgcca cgacgagcag gccgagccaa cggtcatcgc ccatgtccac gagccggcgg
     4321 gcctcccagt cgttgcccac gatcgcgagc gccgccgcgg tcgatccgac gatcgccgcg
     4381 atccagccga gcgtcggcgc ccacgcctgc gtgagcagga ggaccacgag ggcgcccgcg
     4441 gcccacagca ggggcaccac cgccgagacc gacggcccgg gaaggtggta cgggttcgcg
     4501 aggcggagca cgatggcccc ggcgaccagc gcgccggtcg cgacgaccgt gagccgcagc
     4561 ggccgcacgg tcgtcagcgc cgccgtcacg ggcggctctt gtcggggacc cagtcctcgc
     4621 gcagcgcgag caccgcgagc tccgtgcgtg acaggacgcc atagcggtcg aaaagacgcc
     4681 gcaggtggct ctcgacggtc ttctcgctga cgccgagccg cgcgccgatc tcgtcgttcg
     4741 aggcgccgcc gcagaccagc gccaggacct cgatctcgcg gtccgagggt cgtcgggggg
     4801 cgctgcggat ggcgcgcagc atcgaggccg agaacgccgt cccgccgccg gccaccgtgc
     4861 ggatcgcgtc gaggatctcg gggacctcgc tcgtcttgag gaggtagccg gctgccccgc
     4921 gctcgaacgc cgtgcgcacg agcggcggct ggtcgaacga cgacaggagc aggaccgacg
     4981 gcccgtcggg cccgacggcc tcgaggaggc gcaggccctc ggcctcgccg tcgagctgga
     5041 cgtcggtgac gacgacatcg atgcccgtgg tgaccgccag cagcgcccgc gcgtcgtcga
     5101 gggagcgggc cgagcccgcg aggatcaggt cgggcgcgga tcgcacggcg ctgcccacgg
     5161 cgtcgaggat ggcggggtga tcgtccacga gcacgacgcg gatcggctgt cctgtcacgc
     5221 ccgcagccta gcgcgacgca tggcgggcgc gaaggccctc gcgcagggat gacgacgcat
     5281 gggggctgcg ggattccccg cagccgagaa cacgggcata ctcggggtca gggagggtcg
     5341 gcagatgaac gaggacgagg cgacgccggg cggcgacgcc accccgggta gcgacgcacc
     5401 ggcgggtgcc cccccgccgc ccccggccta cccggtcgct cccgcgtacc cgccgtcccc
     5461 gcagtacccg gcctggatcg cgccggtcag cgcccatacc ggcccgcgca tcgacgtggg
     5521 ctcggtcctc ggtcgcacct tcgatacctt cggccgggag tggtcgctgt tcctcgtgct
     5581 cgcgattccc gcggccgtcg ccagcgccct gcggctcgtg atcacgccca ccttcgaatc
     5641 gcaggtccag aaccggttcg ccgcgacgac caacatcggt gacccgtggg cgctgttcgt
     5701 ggtcagcgtg atcgtggcca cggcggtcgg gttcagcgcc ctcgccagcg tggtcgcggc
     5761 ggatgggctg tggcgcggcc agccggccgg gatcgtcgac accgcctcga cggtcgtgcg
     5821 gagcatcccg cgagcgatcc cggtgtggct gttgatcgtg ctggcccagg cggcgatcgt
     5881 cgtgttcacg acccagctca cgccgcagac gccggtcacc cgccagtcgc tggccgcctc
     5941 gagcggcgcg gccgcgctcg tgctgctcgc gatcccgctc gtgatcgtcg gggcgatcgt
     6001 cgccgtcgtg atccaggcac gcctgtcact cgtgctgccg gtgatcgcgc tcgaggaggg
     6061 ctcgcccggc agcgtcattc cgcgcacgtg gcggctgacc aagggcaacg tgatcatgct
     6121 gttcggctgc tccctgctca tcgggctgtg cgtctcggtc acggcctggg gctcgaccct
     6181 gttcctgctg ttcggcgcca accgcctcat cgcgggtgtc gcccttgcca tcgcggagct
     6241 cgtgaccgcc ccgctgaccg ggatctgggt ggcgatcgcg tggggcgacc tcgtcggcgg
     6301 ccggcacgcg gacaacgagc tcatggcgcg tggtcgcggc cggtggacga cggcgacgat
     6361 cgtgttcggg ctgggggcgc tgctggtcgt cgccggcctc ggtatcaccg gagcggccgt
     6421 cacgaggctc gtcgacgcgc cgtagcctcc tgcgcgcccg cgggccatcg agtcgcttcc
     6481 gtcaattccg cgtctcgtga ctgttcgaca gcgccggaga tgcttcgcag cctccgacgg
     6541 gtccgcgatc gcgagtgtcc agcacgacga cccgcctcga ccgctcgcgc gaacccacag
     6601 tccggtgacg aacagtcacc accgcaggat ttggcaggaa cgaagggcgg gaacggcgcg
     6661 ctcggggcga aatgaggacc ggtctgagga gttgccggat ggatacgcag tcgggcgagc
     6721 ccatcgtcgc tgcatgagat cgatcgacgt gatcggtggc caccaaggcc actgacgcga
     6781 cgacgactcg caaggagcag atcgatggac gatcgcacgc agcccggccc cgaccagggc
     6841 aggaagttcc ccgagcccgg ccccgaccag gggaagaagt tccccggccc ggacagcggc
     6901 ttgtatcgcc gcgaggccgg ccccgagcgc ggcttctacc gccgctgagc cgacgacaga
     6961 gacgaggagc agatcgatgg acgatcgcgc gcagcccggc cccgagcagg gcttcaagat
     7021 ccccggaccc gtccggggct tctaccggca cttcaccggc ccggacggcg gctggtaccg
     7081 ccgcttctcg cccgatggcg gcatcttccg ccgcgaggcc gggccggatc gcgggatgta
     7141 ccgcggccac tgacgaacgc cgccccgagc cgcctcgacc aacaggtcgg ggcggctctt
     7201 cgattcgtgg cgaccacgca gaaatgagga acggcctgag gagtagcccg atggagatcc
     7261 ggcgccgcga gcccatggtc ggggcatgag atcgatcgac gtgatcggtg gccaccaagg
     7321 ccacggacgc gacgacgact cgcaaggagc agatcgtgga cgatcgagcg cagccgggtc
     7381 ccgagatggg ccgcaagtac cccgagcccc gccccgagca gggctggagg ttccccgagc
     7441 cc
//
LOCUS       DBNHXO010000502         1639 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3731806, whole genome shotgun sequence.
ACCESSION   DBNHXO010000502 DBNHXO010000000
VERSION     DBNHXO010000502.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3731806"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1639
                     /locus_tag="AC162A_10105"
     CDS             <1..>1639
                     /locus_tag="AC162A_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010043027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HIR5011240.1"
                     /translation="DWGEADVPGCWTMQDTWDHPIYTNVQMPFPHRPPETPEENPTGV
                     YECSFEVPPAWAGRRVVLHVGAAESVLLVELNGQEIGISKDSHLAAEFDVTPSLRPGS
                     NDLRLTVVKWSDASFVEDQDQWWHGGITRPVYLYATGQVYLADLAIDTGLDADGTTGT
                     ISIGAGLGWGPDGPEAGWRVEATVEGLATPLEAEVRHAPPPPGKPSDWTVPGPPRRGV
                     IDLQSLNAAGALTAPDDVDRWREAEPQVRPGRVGRVRLDARLPDITPWSAEVPALYGV
                     AVSLVSPEGQVVERVERRIGFRRVEVRGVELLVNGRAVLIRGVNRHDFDPRTGRVVAA
                     EDMRADVIAMKRWGFNALRTSHYPNDPVLLDACDELGLYVIDEADIESHGWYEDVCAD
                     PRYRLQFVERVARMIERDRHHPSIIAWSLGNESGYGVNHDAAAGWARVADPSRPLQYE
                     GAIKFDWSSGQTASDLVCPMYPPIAALVAHATSGAQRHPLVMCEFSHAMGNSNGTLAE
                     YWDAIESTPGLQGGFIWEWRDHGLVQRLPNGSTRHAYG"
BASE COUNT          237 a          581 c          605 g          216 t
ORIGIN      
        1 ccgactgggg cgaggccgac gtccccggct gctggacgat gcaggacacg tgggatcacc
       61 cgatctacac caacgtccag atgccgttcc cccatcgccc gcccgagacg cccgaggaga
      121 acccgaccgg cgtctacgag tgctcgttcg aggtcccgcc cgcctgggct ggccggcgcg
      181 tggtgctgca cgtcggcgcc gccgagagcg tgctgctcgt cgagctcaac ggacaggaga
      241 tcgggatcag caaggactcg catctcgcgg cggagttcga cgtgacgccg tcgctccggc
      301 cgggctcgaa cgacctgcgg ctcacggtcg tgaagtggtc tgacgcgtcc ttcgtcgagg
      361 accaggacca gtggtggcac ggcggcatca cgcgccccgt atacctctac gcgacgggac
      421 aggtgtatct cgccgacctc gcgatcgaca ccggcctcga cgcggatggc acgacgggca
      481 ccatctcgat cggggccggg ctcggctggg gaccggacgg acccgaggcc ggctggcggg
      541 tcgaggcgac cgtcgagggc ctggcgacgc cgctcgaagc cgaggtgcgc cacgcgcctc
      601 cgccgcccgg caagccgagc gactggaccg tgcccgggcc gccgcgccgt ggcgtgatcg
      661 acctccagag cctcaacgcc gccggcgccc tgaccgcgcc cgacgacgtc gaccgctggc
      721 gcgaggcgga gccccaggtc cggccgggcc gcgtgggacg ggtgcggttg gacgccaggc
      781 tgccggacat cacgccgtgg tcggcggagg tgcccgcgct ctacggcgtg gccgtctcgc
      841 tggtctcgcc cgaaggccag gtggtggagc gggtcgagcg ccggatcggg ttccggcggg
      901 tcgaggtgcg cggggtcgag ctgctggtca acggccgggc cgtcctgatc cgcggcgtga
      961 accgccacga cttcgacccg cggaccgggc gcgtcgtcgc cgccgaggac atgcgcgccg
     1021 acgtcatcgc gatgaagcgc tgggggttca acgcgctgcg cacctcgcac taccccaacg
     1081 accccgtgct gctcgacgcg tgcgacgaac ttgggctgta cgtgatcgac gaggcggaca
     1141 tcgagtcgca cggctggtac gaggacgtat gcgccgatcc gcggtaccgg ctgcagttcg
     1201 tggagcgcgt ggcccggatg atcgagcgcg accgccacca cccctcgatc atcgcctggt
     1261 cgctgggcaa cgagtcgggc tacggcgtca accacgacgc ggcggccggg tgggcgcgcg
     1321 tcgccgaccc gtcgcggccg ctgcagtacg agggcgcgat caagttcgac tggtcgagcg
     1381 gccagacggc gagcgacctc gtgtgtccga tgtacccgcc gatcgcggcg ctggtcgccc
     1441 acgcgacgag cggagcccag cgccatcccc tcgtgatgtg cgagttcagc cacgcgatgg
     1501 gcaacagcaa cgggacgctg gccgagtact gggacgcgat cgagtcgacg cccgggctcc
     1561 agggcggctt catctgggag tggcgcgacc acgggctcgt ccagcggctg cccaacggct
     1621 cgacccgcca cgcgtacgg
//
LOCUS       DBNHXO010000503         3730 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3535148, whole genome shotgun sequence.
ACCESSION   DBNHXO010000503 DBNHXO010000000
VERSION     DBNHXO010000503.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3535148"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /locus_tag="AC162A_10110"
     CDS             complement(<1..1398)
                     /locus_tag="AC162A_10110"
                     /inference="COORDINATES: protein motif:HMM:NF023418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TgpA N-terminal domain-containing protein"
                     /protein_id="HIR5011241.1"
                     /translation="MIVPRIRRPEEGWLTLALVALLIAVVASAVDDPAWVNGRGKLTD
                     GLVLFGMLGVAVGFIGPKVGWSRWTTHIVGALFAGLLIPIVAGWAWLPNPSIGTAFYA
                     TASHSVEAYLDLAWRSRPFTTQELHYVLVLGIVMWGTAQFASYAVFGHRRPLNAVIMT
                     GVVLLANMSMTVRDELPYLILFTAGSLFLLIQMHAFDERATWIRRRIGDPSTISSLYL
                     RGGTVFIVAALVGSLVLTTRAASSPLAGAWDGVHDQLIDLGETIGRFLPVGGDAKGTG
                     VSFGSAARISGKWFSDENTAFSATLPPGTRKGLYWRAVTFNAFNLSGWEKTNGRDVPV
                     EAGAPMLVGTAENPQTVLTKPLKVIVHPEDFRGNELLTLGAPTVVDHPSTVKVAGVQG
                     WFTGVDLPFGSGDYTVDARLLRLDETDVISGNRLIAATEVYPKEITELYTDVPPDTLG
                     VDAELLLEQVKRQARS"
     gene            complement(1395..2609)
                     /locus_tag="AC162A_10115"
     CDS             complement(1395..2609)
                     /locus_tag="AC162A_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR5011242.1"
                     /translation="MFRRLQLLILSVVLVVAAFSTGYGFLFFLVYIGLLVGGGSYILT
                     RLGLSDLEAGYAVSQLSGHVGDKLRVTYTLRNTGRFPKPWLEIYNPTTIPGGLPGRAL
                     SLRGRQERSWLVRTPLLRRGHFRIEPLMIRTGDPFGFFEASAGVGQGVTLVVYPRVDA
                     LPMWRLPPASIEGARASPERTLQTTPLATTVRPYAPGDSMNRIHWPTTARMGEIQVKE
                     FDLEQTADAWLFLDLDASVEAGEGDRSTTEIAVRAAASIADKALGENRAVGMSASGHR
                     LAVLPADRGARQRLKILQLLAAVEADGRTPLAEALVTGVSRLRRGMTAIVITATLDPS
                     FVRPLATLRTRGIGTVVVLLDHLAFAPPADEAAAEVARQRSRAVRHALAEFEVPVSVV
                     GPDQELAEALAR"
     gene            complement(2652..3638)
                     /locus_tag="AC162A_10120"
     CDS             complement(2652..3638)
                     /locus_tag="AC162A_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5011243.1"
                     /translation="MSNIQQVGDTVLANVERVIVGKHQEVRLALVALMCRGHLLIEDV
                     PGTGKTMLAKAIARSLGCSFRRIQFTPDLLPSDVTGLSIYNQKTQEFEFRPGPIMSQV
                     VLADEINRATPKTQSALLECMEERQSTIDGVSHPMPDPFLVIATQNPIEYEGTFALPE
                     AQLDRFMLRIRLGYPQLVDEIVILDEQKRTHPLEDLTEVCSVDELRALQAGVREVYVD
                     PAVSDYIVRLVSSTRTHPDVYLGASPRGSIALYRATQALAGLLGRDYVIPDDVKTLAE
                     PALAHRVIIKTSSSIHDVSAGNVIRELLESTPIESVRQTGGPREVSPSAAAS"
BASE COUNT          564 a         1278 c         1345 g          543 t
ORIGIN      
        1 cgatcgggcc tggcgcttga cctgctcgag gaggagctcc gcgtcgaccc cgagggtgtc
       61 gggcggcacg tcggtataga gctcggtgat ctccttgggg tagacctcgg tcgccgcgat
      121 caggcggttg ccgctgatca cgtccgtctc gtcgaggcgc agcagccgcg cgtcgacggt
      181 gtagtcgccc gaaccgaacg gcaggtcgac gcccgtgaac cagccctgga cgccggccac
      241 cttgaccgtg ctcgggtggt cgaccacggt cggcgcgccg agggtcagca gctcgttgcc
      301 gcggaagtcc tcggggtgca cgatgacctt gaggggcttc gtcagcacgg tctgtgggtt
      361 ctccgcggtc ccgaccagca tgggcgcgcc cgcctcgacc gggacatcgc gaccgttggt
      421 cttctcccag cccgagaggt tgaacgcgtt gaacgtgacc gcccgccagt agaggccctt
      481 cctggtgcct ggcggaagcg tcgcgctgaa cgccgtgttc tcgtcgctga accacttccc
      541 cgagatccgc gccgccgacc cgaagctcac gccggtgccc ttggcgtcgc cgccgaccgg
      601 caggaaccgt ccgatggtct cgccgaggtc gatgagctgg tcgtgcacgc cgtcccacgc
      661 tcccgcgagc gggcttgacg ctgcgcgggt ggtcaggacc agcgacccga ccagggcggc
      721 gacgatgaac accgtgccgc cgcgcaggta gagcgacgag atcgtgctcg gatcgccgat
      781 ccggcggcgg atccacgtcg cacgctcgtc gaacgcgtgc atctggatca ggaggaacag
      841 ggaccccgcc gtgaacagga tgaggtaggg cagctcatcg cgcaccgtca tcgacatgtt
      901 cgcgagcagg acgacgcccg tcatgatcac agcgttcagc gggcggcgat gtccgaacac
      961 cgcgtacgac gcgaactgcg ccgtccccca catgacgatg cccaggacca gcacgtagtg
     1021 cagctcctgg gtcgtgaatg gccggctccg ccaagccagg tcgaggtacg cctcgacgct
     1081 gtggctggcc gtcgcgtaga aggcggtgcc gatcgacggg tttggcagcc aggcccagcc
     1141 ggcgacgatg gggatcagga ggcccgcgaa cagcgctccg acgatgtggg tcgtccatcg
     1201 gctccagccg accttgggac cgatgaaccc gaccgcgacg ccgagcatcc cgaacagcac
     1261 gagcccgtcc gtgagcttgc cacggccatt cacccaggcg ggatcatcga ctgccgacgc
     1321 cacgacggcg atcaggagcg caacgagcgc cagggtcagc cagccctcct ccgggcggcg
     1381 gatccgcgga acgatcatcg ggcgagcgcc tccgccagct cctggtccgg gcccacgacc
     1441 gagacgggga cctcgaattc ggcgagcgcg tgacgcaccg cgcggctccg ctgccgggcg
     1501 acctccgcag cggcttcgtc cgcgggcggc gcaaacgcga ggtggtcgag cagcacgacg
     1561 accgtcccga tcccccgcgt ccgcaaggtc gcgaggggac gcacgaacga cgggtcgagc
     1621 gtcgcggtga tcacgatcgc cgtcatgccg cgccgcaggc ggctgacgcc cgtcaccagc
     1681 gcctcggcca gcggggtccg cccgtccgcc tcgaccgccg ccagcagctg gaggatcttg
     1741 aggcgctggc gcgcgccgcg atcggcgggc aggacggcca gtcgatggcc gctcgcggac
     1801 atgccgaccg cgcggttctc gccaagggcc ttgtccgcga tggatgcggc cgcgcggacg
     1861 gcgatctcgg tcgtcgaacg atcgccctcg ccggcctcaa ccgacgcgtc gaggtcgagg
     1921 aacagccacg cgtcggcggt ctgctcgagg tcgaactcct tgacctggat ctcgcccatg
     1981 cgcgccgtgg tcggccagtg gatccggttc atcgagtcgc ccggtgcgta cggccggacc
     2041 gtcgtggcca gcggggtcgt ctggagcgtt cgctccggtg acgcgcgggc gccctcgatg
     2101 ctcgccggtg gcaggcgcca catcggcagc gcgtccaccc gggggtagac gacgagggtg
     2161 accccctgcc cgacgcccgc cgaggcctcg aagaagccga acgggtcgcc cgtccggatc
     2221 atcagcggct cgatccggaa gtgcccgcgt cggagcagcg gcgtgcgcac cagccacgac
     2281 cgctcctggc gcccgcggag cgacagcgcc cgtcccggca gaccgcccgg gatcgtcgtc
     2341 gggttgtaga tctccagcca cggcttcggg aagcggccgg tgttgcgcag ggtgtacgtg
     2401 acccgcagct tgtcgcccac gtgaccgctc agctgcgaca cggcgtagcc ggcctcgagg
     2461 tcggacaggc cgagccgggt caggatgtag ctcccgcccc cgaccagcag cccgatgtac
     2521 acgaggaaga acaggaaccc gtagccggtc gagaacgccg ccaccacgag gacgacggag
     2581 aggatgagca gctggaggcg ccggaacaag gcggtcggtc ctccgtggat cgcggggtcg
     2641 tggtggacgg atcagctggc ggcggcgctc gggctgacct cgcgcggacc gcccgtctgg
     2701 cggacggact cgatcggggt gctctcgagg agctcccgga tcacgtttcc cgcgctgacg
     2761 tcgtggatgg acgagctggt cttgatgatc acgcggtgtg caagcgcggg ctcggcgagc
     2821 gtcttgacgt cgtcggggat cacgtagtcg cgacccagca ggccggcgag cgcctgggtg
     2881 gcccggtaca gggcgatcga tccgcgcggg ctggcgccga ggtagacgtc cgggtgggtc
     2941 cgggtcgacg agacgaggcg gacgatgtag tcgctgaccg ccgggtcgac gtacacctcg
     3001 cgcacgcccg cctggagggc gcgcagctcg tccaccgagc agacctcggt caggtcctcg
     3061 agcggatggg tccgcttctg ctcgtcgagg atcacgatct cgtcgaccag ctgcgggtag
     3121 ccaagccgga tccgcagcat gaaccggtcg agctgggcct ccggcagcgc gaacgtcccc
     3181 tcgtactcga tcgggttctg ggtcgcaatc accaggaacg ggtccggcat cggatggctg
     3241 acgccatcga tggtcgactg gcgctcctcc atgcactcga gcagcgcgct ctgggtcttg
     3301 ggcgtcgccc ggttgatctc gtcggcgagc accacctggc tcatgatcgg cccgggccgg
     3361 aactcgaact cctgcgtctt ctggttgtag atcgacaggc cggtcacgtc cgacggcagc
     3421 aggtcgggcg tgaactggat ccggcggaag gagcagccga ggctccgggc gatcgccttg
     3481 gccaacatgg tcttgccggt gcccggaacg tcctcgatca ggaggtggcc gcggcacatc
     3541 agcgcgacga gggcgaggcg cacctcctgg tgcttcccga cgatgactcg ctcgacgttg
     3601 gcgaggacgg tgtcgccgac ctgctggatg ttgctcatcg gactcccgtg gtgtgcggcg
     3661 cgacgccgcg ggctgcgcgt gtccggcgtg aacgtgccgg tcgggcggtg cgatgacgtc
     3721 gcgctgagcg
//
LOCUS       DBNHXO010000504        11987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2741695, whole genome shotgun sequence.
ACCESSION   DBNHXO010000504 DBNHXO010000000
VERSION     DBNHXO010000504.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 11987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11987
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2741695"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /locus_tag="AC162A_10125"
     CDS             <1..1303
                     /locus_tag="AC162A_10125"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR5011244.1"
                     /translation="DPDALGRWPTADDLERAAPGRRVALWAHDHHALLASARALAEAG
                     VSGDRSDPPGGVIRRDGAGRPTGVLHESAAGLVATVVPPPSVDAMAAAVRTLARELLA
                     LGVVAVHDPGTLAARGDLGPFEAYRRLAASGDLALRVHASIRASQLGAAREAGLRSGL
                     PLGPDPLDRLRLGWLKAFADGSLGSRTAALLEPMERVAGEAAPPNDGLGVWLASPDHL
                     RELAARAAAAGIATQIHGIGDAAVRAALDALAPTVGVTPLMPRVEHAQLVADDDVARF
                     AELGIAASVQPVHVRSDVDKARAMWGGERAERRAFPLAALSRTGAVVAIGTDAPIEPI
                     DPWPGIACAVTRSAPSWPAGTAPLGSAQSLPLWRAIRAACVDPAISAGELDRGRLVAG
                     HRADVVVLPAAAISEPVDTGGGLWHARPRLVLVDGVVVAGG"
     gene            complement(1311..2687)
                     /locus_tag="AC162A_10130"
     CDS             complement(1311..2687)
                     /locus_tag="AC162A_10130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase family protein"
                     /protein_id="HIR5011245.1"
                     /translation="MPSFDFVVIGAGPAGEGTAHKARELGATVAVIDRRWFGGSCPHI
                     GCVPSKALLYAAQRHHAGADYSWQRASDRRDYMVNRAPGAPEPDDTSHVRSLERAGVS
                     TFRGEGRITGRGRVTITGDDGATTELDARHVVIAVGSTSKVPPLEGLADTRPWTNEQA
                     TLTRELPRSLLVLGGGPTGCELAQVFVRFGVPTVIVQSGPRLVPTEHPRNAETVRFAL
                     ERDGAVVRTGVRAVRARAGAGIDGAHVVELDDGSTAEGHVILLAVGRAFPFEGLGLEH
                     YDVAVSGPDAYKRDGRLRLADGLWAAGDPAGPELHTHQGHYQGELVARMALGEAIVPD
                     YRALPRATYMEPESASVGVSLDQATAAGIDAFECVADFTRSAKGYSVEAETGHVTIVV
                     DRATRELVGAAMACPDASAAIHECVLAIKARVKVDVLAETIHAFPSTSRILNGLFAEA
                     RSRLNEAA"
     gene            complement(2692..3504)
                     /locus_tag="AC162A_10135"
     CDS             complement(2692..3504)
                     /locus_tag="AC162A_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR5011246.1"
                     /translation="MADVALSSTRNPRVRAAAALRDRRARDEAGLTLIDGIRELTRAI
                     AGGARVVEVYVDPDRIGAPAAAVVEAARAVGAEVIEVSGTVIDTLAYGDRGEGVVAVA
                     RIPDTSLDAVSLPAGALVVVVEGVEKPGNLGAVLRSADGAGADLVIAADPRTDLFNPN
                     AIRASQGTVFSIPVAAAPSAAVRTWLREARLQVVTARVDAPTAYTDADLRGAVAIVLG
                     SETSGLTDTWSGDDVAPVRLPMLGVADSLNVSIAAAVLLYEARRQRASVQAD"
     gene            complement(3526..4347)
                     /locus_tag="AC162A_10140"
     CDS             complement(3526..4347)
                     /locus_tag="AC162A_10140"
                     /inference="COORDINATES: protein motif:HMM:NF020996.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR5011247.1"
                     /translation="MGDLELIDCGDGRRLERFGDVIVDRTAPSAVMPRRLTAAEWRRA
                     PLRWTAGAWARNGDRPGWTVNVGGLQLECRPAAGGQLGLFPEHAATWGWLDGAVRAAG
                     AGLGRPPELLSLFGYTGGATLACARAGARVTHVDASRPAVAWARRNAEASGLADLPVR
                     WLADDVRTVVRRERRRGKVYDGVVVDPPSYGHGVSPWKIDTDLPPLLDDLAALLGPRP
                     GLVLLTAHTPGFDGERLAALCREHLGVAAGSGEMRLTATSGNVLRLGSWARWPAR"
     gene            5217..5762
                     /locus_tag="AC162A_10145"
     CDS             5217..5762
                     /locus_tag="AC162A_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011248.1"
                     /translation="MATPIAPASRSSRLPFRPVAAILTGIALFAFGVFIGLEVLTPEP
                     ITAGRGTDVTLPTPSPTPIVHTLTVNVAIDESRGSLEDTLGTIAMGAPCEPSAGTFPD
                     LRPGTPVLVADQAGTIIAKASLSEGRKSGVSTCAFTAKAPVPEATFYRIVLGDRAANV
                     LAIDALSSAAWTTELRFVARP"
     gene            complement(5777..6097)
                     /locus_tag="AC162A_10150"
     CDS             complement(5777..6097)
                     /locus_tag="AC162A_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011249.1"
                     /translation="MTVLLVIAAQLVDLASFGVVARLLGPDGEVGPLGQVYAAGGFAP
                     VAATKILGLLAVMIILALYARKVGSPRRLALMVAAVGLFGALTNVMGMLGPGVAAATT
                     ALGF"
     gene            6336..6412
                     /locus_tag="AC162A_10155"
     tRNA            6336..6412
                     /locus_tag="AC162A_10155"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6370..6372,aa:Val,seq:gac)
     gene            6699..8564
                     /gene="thrS"
                     /locus_tag="AC162A_10160"
     CDS             6699..8564
                     /gene="thrS"
                     /locus_tag="AC162A_10160"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR5011250.1"
                     /translation="MAEDQAGAAAVAGEDDEPEFEAPQRGSVDELLDAGAHAPIDAMR
                     HSTAHVMAEAVLDLFPGAKLGIGPAIDNGFYYDFELPRALSPDDLAAIEARMGESIKA
                     DHPFVRKELPFDEARAIVDGEGQAFKVEILDDLRAKAEAAGEPAPVTTFYEHGPFHDL
                     CRGPHVESTGKIGPFKLLSVSGAYWRGDQARASMQRIYGTVWETQEELDQFLWRREEA
                     KKRDHRRLGVQLDLFSFHDVAPGSAFWHPKGWRLYQTLRGAMRELQDRRGYEEIYTPP
                     LVHQKLWEQSGHWALYRDNMYLVDVEGQWFSLKPMNCPESTFIYRSRLRSYRDLPLRL
                     SEYGVLHRNELSGVLSGLTRVRHFVTDDAHIYVRPDQIAAEIEALMGELRESYGWFGL
                     TPTLTFGTRPDKALGDPAVWAETEAIMRAELDRSGLQYRVKEKDGAFYAPKLDIQITD
                     ALGREWQTATIQVDRVMLPEQFDLHYIDENGQQQRPVAIHRAIYGSFERFIGVLTEHF
                     AGAFPLWCAPVQAVVIPIADRHVEAAEALAVELRAAGVRFVEVDASDNRMQNKIRLAQ
                     GQKVPYMLILGDREVEARTASVRRRGATKDEPQETLAWSDLAARLGAEIRERRPG"
     gene            complement(8570..10144)
                     /locus_tag="AC162A_10165"
     CDS             complement(8570..10144)
                     /locus_tag="AC162A_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011251.1"
                     /translation="MRASSPRATFAERLGVDRTLLLQLAGLAVAAFLIRLVPMLLTGG
                     VRGFVDYDDGVYMGTALALADGRVVYRDFPMLHPPGIVYVLSPFAALSWLVSDSTAFA
                     AARVGFMVLGGLNTFLVGLVGSRGGRSTALAAAAMYAVWIVAARVERSTWLIAPQTAL
                     LLLALLALTYPRSEASPTVTWRKALLVGALLGASCAIQIWGVVVAAVVFVWLLLRVWR
                     QPGGWLRPMVAYALGGIGAVALAFLPFLAAAGEKMVRLVIFDQIGRGAGGPPIPNRIR
                     AMEGLPSGFADLPLGGLIPIVIAVVVVVAVLWLAWRRPVVRLWVALFLAQCGFLLVTP
                     SYFGHYSGWMAAAAALSIGGVAAALIELPGLSVRASRAVGAVYVAGLACLLVVAVWPR
                     LVGFPRPSVPFPSRAVGELIADARCPTSDNPTVLILTNTLQRVLNNGCPLLVSPTGVS
                     YDTDRDLTGKDRTRPRQVEYQAIMRDYFSGSDAALFIRPASALGLTEETWAPIRARLP
                     VERQIGDLRIFLPAGN"
     gene            10373..11026
                     /locus_tag="AC162A_10170"
     CDS             10373..11026
                     /locus_tag="AC162A_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011252.1"
                     /translation="MAVTGAFLLAACAASTPTPAPDDSAGAASPGTTSSAGEPSAADV
                     GAASSLVSAVRLDSPDSLASIEGVRFTPAGSKAAADLLASGASGDALWAATYVYASSG
                     DSPEPLRAIATNTGASPSVRVMAAAGLLGRGDIAGFEPLIAALAPSDPMDGMEPAGTT
                     SEFAADVLERYAKAGFGPALTASEDERAASQAQWRAWLDANRATLQFDPASESWVTK"
     gene            11023..>11987
                     /locus_tag="AC162A_10175"
     CDS             11023..>11987
                     /locus_tag="AC162A_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011253.1"
                     /translation="MRRLPLIVAAAFALVLFSSAGSLVQADTTFTPSGAVTFKVDHDA
                     KTITATVKLAFYNRSCGVGQSCDAPVGDVARIVRAIEAMWNTGQKVKCYTFSVVVDGR
                     TVSSQADAGRNEVDVGLDYGPVPVRAFVHGEYNVATTTDPLSGSPDNRIDPVHDPASP
                     TTWPGRTYDQTYAHEFGHILGLDDNYDPARPGFPVPGTSDDLMFRKQGDVTAEMVKRV
                     VERSGQVELEDLECGWTTNADTPLGTVRGTHCEGLGGEWTINGDEKLLSRGTVTSLWN
                     VTIDPTTLAGKFRHEKIQDIKPTITTSNQKGSARIVKGQDGRVTMT"
BASE COUNT         1765 a         4345 c         4344 g         1533 t
ORIGIN      
        1 ggacccggac gcgctcgggc gctggccgac ggccgacgac ctcgagcgcg cggcgccggg
       61 ccggcgtgtc gcgctgtggg cccacgacca ccacgcgctg ctggccagcg cccgtgccct
      121 cgcggaagct ggcgtctcgg gcgaccgctc cgacccgccg ggcggcgtga tccggcgaga
      181 cggggccggc cggccgacgg gcgtgctcca tgagtcggcg gccggcctcg tggccacggt
      241 cgtcccgccg ccgtcggttg atgcgatggc ggccgccgtg cgaacgctcg cccgggagct
      301 cctcgccctg ggtgtcgtcg cggtccacga cccgggcacg ctggcggccc gcggcgacct
      361 cggtccgttc gaggcgtatc ggcggctcgc cgcgagcggt gacctcgcgc tgcgcgtcca
      421 cgccagcatc cgcgcgtcac agctcggtgc ggcacgggag gccggactcc gcagcgggct
      481 gccgttgggc ccggacccgc tcgatcgcct gcgcctgggc tggctcaagg cgttcgccga
      541 tggctcgctc ggatcgcgga ccgccgcatt gctggagccg atggaacggg ttgcgggcga
      601 ggccgcgccg cccaacgacg ggctcggcgt gtggctcgcg tcgcccgacc acctgcgcga
      661 gctcgccgct cgggcggccg ccgcgggcat cgcgacgcag atccatggga tcggcgacgc
      721 cgcggtccgg gcggcattgg acgccctcgc gccgaccgtc ggcgtgacgc cgctgatgcc
      781 gcgcgtcgag cacgcgcagc tcgtggccga cgacgacgtc gcgcggttcg ccgagctcgg
      841 catcgccgcg tccgtgcagc cggtgcacgt gcgctcggac gtcgacaagg cgcgggccat
      901 gtggggtggc gagcgcgccg aacggcgcgc cttcccgctg gccgcgctgt cccgcaccgg
      961 cgccgtcgtc gcgatcggga cggacgcccc catcgagccg atcgacccgt ggcccgggat
     1021 cgcctgcgct gtcacccgct cggcgccgtc gtggccggcc gggaccgcgc cgctcggatc
     1081 tgcgcagtca ctgcccctgt ggcgtgccat ccgggccgcg tgcgtggacc cggcgatcag
     1141 cgcgggcgag ctcgaccggg gccggttggt ggccgggcat cgggcggacg tcgtcgtgct
     1201 gcccgccgcg gccatcagtg agccggtcga taccggcggg gggctgtggc acgcgcgtcc
     1261 gaggctggtc ctggtcgacg gggtggtcgt cgcgggcggc tgaccctgtc tcaggcggcc
     1321 tcgttgagcc tcgagcgggc ctcggcgaac agcccgttga ggatccgcga cgtgctcggg
     1381 aacgcgtgga tcgtctcggc gaggacgtcg accttcacgc gtgccttgat cgccagcacg
     1441 cactcgtgga tcgcggcgga cgcgtcgggg caggccatcg ccgcgccgac gagctcgcgc
     1501 gtcgcgcggt cgaccacgat cgtcacgtgc ccggtctctg cctcgaccga gtagcccttg
     1561 gcgctcctcg tgaagtcggc gacacactcg aacgcgtcga tcccggccgc cgtcgcctgg
     1621 tcgaggctga cgcccacgga cgccgactcg ggctccatgt acgtcgcccg cggcagggcc
     1681 cggtagtcgg ggacgatggc ctcgccgagg gccatgcggg cgaccagctc gccctggtag
     1741 tggccctggt gggtgtgcag ctccggcccg gccgggtcgc cggcggccca cagcccgtcg
     1801 gcgagccgca accgcccgtc gcgcttgtac gcatcgggcc cggacaccgc cacgtcgtag
     1861 tgctcgagcc cgagcccctc gaacgggaag gcgcgcccga cggccagcag gatcacgtgc
     1921 ccctcggccg tcgacccgtc gtcgagctcg acgacgtggg cgccgtcgat tcccgcgccc
     1981 gcccgcgccc tgaccgcgcg aacgccggtt cggaccaccg ccccgtcgcg ctccagcgcg
     2041 aaccggacgg tctcggcgtt gcgtgggtgc tcggtcggca cgagccgggg cccggactgg
     2101 acgatcaccg tcgggacgcc gaaccgcacg aagacctggg cgagctcgca gccggtcgga
     2161 ccgccgccga gcacgagcag gctgcgcggc agctcgcggg tgagcgtcgc ctgctcgttg
     2221 gtccaggggc gggtgtcggc gaggccctcg agcggcggga ccttggaggt ggagccgacc
     2281 gcgatcacga cgtgccgggc atcgagctcg gtggtcgcgc cgtcgtcgcc ggtgatcgtc
     2341 acacgcccgc ggccggtgat ccgaccctcg ccacggaacg tcgacacgcc tgcccgctcg
     2401 agcgagcgga cgtgcgaggt gtcgtcgggc tccggcgccc cgggcgcacg gttgaccatg
     2461 tagtcgcgac gatccgacgc tcgttgccac gagtagtccg cgcccgcgtg gtggcgttgg
     2521 gccgcgtaca gcagcgcctt ggacggcacg caaccgatgt gcgggcacga gccgccgaac
     2581 cagcgacggt cgatcaccgc gacggtggcg ccgagctcgc gggccttgtg ggcggtcccc
     2641 tcgccggccg ggccggcgcc gatcacgacg aagtcgaagc tgggcatgcc ctcagtctgc
     2701 ctgcacgctg gcgcgctgtc gcctcgcctc gtacagcagg acggcggcgg cgatcgagac
     2761 attgaggctg tcggcgacgc cgagcatcgg tagcctgacg ggcgcgacgt cgtcccccga
     2821 ccaggtgtcc gtgagccccg acgtctcgct gccgagcacg atcgcgacgg cgccccggag
     2881 gtcagcgtcg gtgtaggcgg tcggggcatc gacgcgtgcc gtgacgacct gcaagcgcgc
     2941 ctcgcgcaac caggtccgca ccgcggccga tggcgcggcg gcgaccggga tcgagaacac
     3001 ggtgccctgc gacgcccgga tcgcgttcgg gttgaacagg tcggtgcggg gatccgcggc
     3061 gatgacgaga tcggcgcccg cgccgtcggc cgagcgcagc accgcgccga ggttgccggg
     3121 tttctcgacg ccctccacga cgacgaccag cgcgccggcg ggcagcgaga cggcgtcgag
     3181 cgaggtgtcg gggatgcgcg cgacagcgac gactccctcg ccccggtcgc cgtaggcaag
     3241 ggtgtcgatg acggtgccgc tgacctcgat gacctcggcg ccgacggcac gcgccgcctc
     3301 gacgaccgca gccgcgggag ccccgatgcg atccgggtcc acgtacacct cgaccaccct
     3361 ggccccgccg gcgatcgccc tcgtgagctc gcggatcccg tcgatgagcg tgagaccggc
     3421 ctcgtcgcgc gcccgccggt cgcgaagcgc tgccgccgcc cggacgcgtg ggttgcgggt
     3481 gctgctcagc gcgacgtcgg ccacgacgtc atcttcgcag accggtcagc gggcgggcca
     3541 gcgtgcccac gaccccagtc gcaggacgtt gccgctcgtg gcggtgagcc gcatctcccc
     3601 gctgccggcg gcgactccga gatgctcgcg acacagggcg gcgaggcgct cgccgtcgaa
     3661 tcccggggtg tgtgccgtga gcagcacgag accgggccgg gggccgagca gggccgcgag
     3721 gtcgtcgagc agcggcggga ggtcggtgtc gatcttccag ggcgagacgc catgcccgta
     3781 gctcggcgga tccacgacca cgccgtcgta gaccttcccg cgccggcgtt cgcggcggac
     3841 cacggtccgg acgtcgtcgg cgagccagcg cacgggcagg tccgcgaggc ccgatgcctc
     3901 cgcgttccgg cgggcccagg cgaccgcggg tcgtgacgcg tcaacgtggg tgacccgcgc
     3961 gccggcgcgg gcgcaggcga gcgtcgcgcc gccggtgtac ccgaacagcg agagcagctc
     4021 cggcggacgg cccaggccgg cgccggcggc ccggaccgcc ccgtcgagcc agccccaggt
     4081 cgccgcgtgc tcggggaaga gcccgagctg gccgcccgcc gcgggccggc actcgagctg
     4141 gaggccgccc acgttcaccg tccagcccgg tcggtcgccg ttccgcgccc acgccccggc
     4201 cgtccagcgc agcggcgctc gccgccactc tgcggccgtg agccgccggg gcatcaccgc
     4261 gctcggcgcc gtgcggtcga cgatcacgtc gccgaagcgc tcgaggcggc ggccgtcgcc
     4321 gcaatcgatc agttcgaggt cgcccacggc gtcatggtgg catgcatcgc ccgccgaccg
     4381 ggcaagagcg tgtagtgtct gtcgccgcag gcgcgttttg cctgtcgcag caggaaagga
     4441 cgccgccgtt ggacgacctg tgggcgtgcc cgagctgcca ctcgctcaac gttcgaagcg
     4501 cccgtcgctg ctacaagtgc aaggagaagc gcccggccgc tgtcacggcc gatgccgctc
     4561 ctttggatac gacgacgcct gcgccagagc cttcgacaaa gccgaccacg cagaaggcga
     4621 ccacgccaac ggagcctgcg cctgcacccg aggctgctcc ggcagccccg accaaggagc
     4681 cagcgcccaa gagaagggtg ttcgggaggt cggcccgatc ggtcgccgag gggtccgccg
     4741 cggccgcagt ggcgccagac gtgccggtgg cgccagaccc gcccgaggtc ccggttgccg
     4801 caccggaggc gtcagtcatc ccggaggagg cgttggccgc ccaacaggag ccaacagccg
     4861 ccccgtcgga ggccttggcc gatgcggagg agccggcccc agacccgggg gaggcgacgg
     4921 acgacgaacc gcccgtggac gcggccgcac ggctcgcctc agtcgtcggt gacgcccccg
     4981 acgttgccgc agcacccgag ctggttgcca cgcccgagcc gggggcgacg cccgagccgg
     5041 ccccgccgaa gcctgccaac atgccgccac cggtcgactg tgtcgcgtgc ggtaagccga
     5101 tcttcacctc ggaccgctac tgcgccagct gcggccaccg ggtgggagcc ccgctcgcgg
     5161 catcaggggc ggtcggcgca tcagcgttcg cggcgcggcc cgtctggccg cgcgatatgg
     5221 caacaccgat cgcgccggcg tcgcgctcat cgaggctccc gttcaggccg gtcgccgcga
     5281 tcctgaccgg catcgcgctg ttcgcgttcg gagtgttcat cggtctggag gtcctgacgc
     5341 cggagccgat cactgccggc cggggcacag acgtgaccct gccgacgccg agtccgaccc
     5401 ccatcgtgca taccctcacg gtgaacgtgg ccatcgacga gtcgcggggg agcctcgaag
     5461 acacgctggg cacgatcgcc atgggcgcgc cgtgcgagcc atccgcgggc acgttccccg
     5521 acctccggcc cggaaccccc gtcctcgtgg cggaccaggc cggcacgatc atcgccaagg
     5581 cgagcctctc ggagggccgc aagtccggcg tcagcacgtg tgccttcacg gccaaggcgc
     5641 cggtgcccga agcgacgttc taccggatcg tgctcggcga tcgggcagcg aacgtgctgg
     5701 cgatcgacgc cctgtccagc gccgcctgga ccacggagct gcgcttcgtc gcgaggccct
     5761 gatcgagcgt cgccggtcag aagcccaggg cggtcgtggc cgccgccacg cccgggccga
     5821 gcatccccat gacgttggtc agggcgccga acagtccgac cgcggcgacc atcagcgcga
     5881 ggcgccgcgg tgagccgacc ttccgcgcgt acaaggcgag gatgatcatc acggccagca
     5941 gcccgaggat cttggtcgcc gccaccggag cgaagccgcc ggcggcgtag acctggccga
     6001 gcgggcccac ctcgccgtcc gggccaagca ggcgagccac cacgccgaag ctcgcgaggt
     6061 cgacgagctg cgccgcgatg acgagcagca ccgtcacgcg cgccagggac cgccgttcca
     6121 cagccgtcac gtcgtcaccg atccgagggc acaccggccc ggccaacgta gtgtcaggac
     6181 tcaaccggat tgtggcgcac cgcacctatc cgcgcatcac cgcaccgaac tgcacggtgc
     6241 gtgctggcac cactgtcggt gtcctcacgg ggcccgggag gcccgaggcc tgcgccctga
     6301 cgaccacggg cgccgtgcta tcctgcgcgc ccgaagggcg attagctcag ctggttagag
     6361 cgcatgcttg acatgcatga ggtcactggt tcgagtccag tatcgcccac cattccaggc
     6421 ctccgggcct gcggacagat agcgcgtcga gcgggacgcg ccccatcggg acgtcgccag
     6481 agagccgggg ccacggctgc aagccccgac ggacacgacc gatgacggca ccacccgcga
     6541 gcggccggtg aaccggcccg ggatccgccc gttagcgcgg ctcgagcgca cgacggcatc
     6601 gcggcgagcg atgcggccgt gaaggcgggt ggaaccgcgg gaggtcggac ctctcgtcct
     6661 gtcggacggg aggttctgtg attctcggga gaagcgagat ggccgaagac caggcaggcg
     6721 ccgcggcagt cgcgggcgaa gacgacgagc cggagttcga ggcgccgcag cgcggttcgg
     6781 tggacgagct gctcgatgcc ggcgcccacg cgccgatcga tgccatgcgc cacagcaccg
     6841 cgcacgtgat ggccgaggcc gtcctcgacc tgttcccagg cgcgaagctc ggcatcgggc
     6901 ccgccatcga caacggcttc tactacgact tcgagctgcc gcgcgccctg tcgcccgacg
     6961 acctggccgc catcgaggcc cggatggggg agagcatcaa ggccgaccac ccgttcgtgc
     7021 gcaaggagct gccgttcgac gaggcgcgcg ccatcgtcga cggggagggc caggccttca
     7081 aggtcgagat cctcgacgac ctgcgggcga aggccgaggc ggccggtgag ccggcaccgg
     7141 tcacgacgtt ctacgagcac gggccgttcc acgatttgtg ccgcgggccc cacgtcgagt
     7201 cgaccggcaa gatcggcccg ttcaagctgc tctccgtctc gggtgcctac tggcgcggcg
     7261 accaggcgcg ggcgtcgatg cagcggatct acggcaccgt ctgggagacc caggaggagc
     7321 tggaccagtt cctgtggcgg cgggaagagg cgaagaagcg cgaccaccgc cgcctcggcg
     7381 tgcagctcga cctgttcagc ttccacgatg tcgcgcctgg gtcggcgttc tggcatccca
     7441 agggctggcg cctgtaccag accctgcggg gggcgatgcg cgagctccag gatcggcgtg
     7501 gctacgagga gatctacacg ccgccgctcg tgcaccagaa gctgtgggag cagtccgggc
     7561 attgggcgct ctaccgcgac aacatgtacc tcgtcgatgt cgaggggcag tggttcagcc
     7621 tcaagccgat gaactgcccc gagtcgacat tcatctatcg gtcgcggctg cggtcgtacc
     7681 gcgacctgcc gctccgtctg tccgagtacg gcgtgctgca tcgcaatgag ctctcgggcg
     7741 tgctgtcggg cctcacccgg gtgcgccact tcgtgaccga cgacgcgcac atctacgtcc
     7801 ggccggacca gatcgccgca gagatcgagg cgctgatggg cgagctccgc gagtcgtacg
     7861 gctggttcgg cctgacgccg acgctcacgt tcggcacgag gccggacaag gcccttggcg
     7921 accccgcggt gtgggcggag accgaagcga tcatgcgcgc cgagctcgac cgttcgggcc
     7981 tccagtaccg ggtcaaggag aaggacggcg cgttctacgc gcccaagctc gacatccaga
     8041 tcaccgacgc gctcgggcgc gagtggcaga cggcgacgat ccaggtcgac cgcgtgatgc
     8101 tgcccgagca gttcgacctc cactacatcg acgagaacgg ccagcagcag cggcccgtcg
     8161 cgatccaccg ggcgatctac gggtcgttcg agcgcttcat cggcgtcctg accgagcact
     8221 tcgcgggcgc gttcccgctg tggtgcgccc cggtccaggc cgtcgtgatc ccgatcgccg
     8281 accggcacgt tgaggccgcc gaggccctgg cggtcgagct gcgggccgcg ggcgtgcggt
     8341 tcgtcgaggt cgacgcctcc gacaaccgga tgcagaacaa gatccggctg gcgcaggggc
     8401 agaaggtccc gtacatgctg atcctgggcg accgcgaggt cgaggcccgg acggcatcgg
     8461 ttcgccgccg gggcgcgacg aaggacgagc cacaggagac actcgcctgg tccgacctcg
     8521 cggcgcggct gggcgccgag atccgcgaac gacggccggg gtaggaccgt cagttcccag
     8581 ccgggaggaa gatcctgaga tcgccgatct gccgctcgac gggtaggcgc gcccggatcg
     8641 gcgcccacgt ctcctcggtc aggccgaggg cgctcgcggg gcggatgaac agggcggcgt
     8701 cgctgccgct gaagtagtca cgcatgatcg cctgatactc gacctgtcgg gggcgcgttc
     8761 ggtccttccc ggtcaggtcg cgatccgtgt catacgagac gcccgtgggg ctgacgagca
     8821 gggggcatcc attgttcagc acgcgctgca gcgtgttcgt caggatcagg acggtcggat
     8881 tgtccgaggt ggggcaacgg gcgtcggcga tgagctcgcc gaccgcgcgg ctcgggaacg
     8941 gcacgctcgg tcgggggaac ccgacgagcc ttggccagac ggcaacgacg agcaggcagg
     9001 ccagccccgc gacatagacg gcgccgacgg ccctcgaggc acgcaccgag aggcctggca
     9061 gttcgatcaa tgctgcggcg acgccgccga tcgacagggc ggccgcggcc gccatccacc
     9121 ccgagtagtg gccgaagtag gacggcgtga caagcaggaa cccgcactgc gccaggaaca
     9181 gcgcgaccca tagccgaacc accggtcggc gccacgcgag ccacaggacc gccacgacga
     9241 cgacgacggc gatcacgatc ggaatgagcc cgcccagcgg gaggtcggcg aagccggatg
     9301 gcaggccctc catcgcgcgg atgcggtttg gaatcggcgg accgcccgcg cctcggccga
     9361 tctggtcgaa gataaccagc cggaccatct tctcgccggc ggccgccagg aacgggagga
     9421 acgcgagcgc caccgcgccg atcccgccga gcgcgtacgc gaccatcggc cgcaaccatc
     9481 cccccggttg tcgccacacg cgcagcagca gccacacgaa caccacggcc gcgaccacga
     9541 cgccccagat ctggatcgcg cacgacgccc cgaggagcgc ccccaccagc agcgccttgc
     9601 gccaggtgac ggtcggcgac gcctcggaac gcggataggt gagtgccagc agggcgagca
     9661 ggaggagcgc cgtctgtggg gcgatcaacc acgtggaccg ctcgaccctt gctgcgacga
     9721 tccacacggc gtacatcgcc gcggcggcga gcgcggtcga gcgccctcca cgcgagccga
     9781 ccagaccgac gaggaacgtg ttcaggccgc ccaggaccat gaacccgacc cgggcggctg
     9841 cgaaggcggt cgagtccgac accagccagc tgagcgcggc gaacggggac agcacgtaca
     9901 cgatcccggg cgggtgaagc atcggaaagt cgcggtagac gacccgtccg tcggccagcg
     9961 cgagcgccgt ccccatgtag acgccgtcgt cgtagtcgac gaagccgcgc acgccgccgg
    10021 tcagcagcat cggcacgagg cggatcagaa atgccgcgac ggcgagcccg gcgagctgca
    10081 gcagcagggt gcggtcgacg ccgagtcgct ccgcgaaggt cgcgcgcggc gacgaagccc
    10141 gcatgccttc ctccagttcc gggcggctcg cacgccggcg tccgagacat cgccgccagt
    10201 agcctacgtc gtcctctcga catcgtgccc cgcgccggcg gagactgtgt cggccggcgc
    10261 gatcagcgag tctcccgggc cgggacgtcg cgtacactcg gccctcacgc atcgtccggc
    10321 gatcggggaa cgaatggcgc gcagcaggag tcttccggcg ccctgggcgg tcgtggccgt
    10381 cacaggtgcc ttcctgctgg ccgcctgtgc cgcgtcaacc ccgacgcccg ccccggacga
    10441 ctccgcgggc gccgccagcc cgggcacgac ctcgtcggcc ggcgagccga gcgcagccga
    10501 tgtcggcgcc gcctcctcgc tcgtctcggc cgttcgcctg gacagccccg actcgctcgc
    10561 gtcgatcgaa ggggttcggt tcacgccggc gggatcgaag gcagccgccg acctcctggc
    10621 gtcaggcgcc agcggcgatg ccctgtgggc cgcgacgtac gtctacgcga gctccggcga
    10681 cagtcccgaa ccgttgcgcg ccatcgcgac gaacaccggc gccagtccgt cggtccgggt
    10741 catggcggcc gcgggcctcc tcgggcgcgg cgacatcgcc ggcttcgagc ccctcatcgc
    10801 ggcgcttgcc ccatccgatc cgatggacgg gatggagccc gcggggacga cgtccgagtt
    10861 cgcggccgac gtcctcgagc gatatgcgaa ggccgggttc gggcccgcgc tgacggcgtc
    10921 ggaggacgaa cgggccgcga gccaggccca gtggcgggca tggctggacg cgaatcgcgc
    10981 caccctgcag ttcgatcccg cgtccgaatc ctgggtgacg aagtgaggcg cctgccgctg
    11041 atcgtcgccg cggcgttcgc cctggtcctg ttctcgagcg ccggctcgct ggtccaggcg
    11101 gatacgacgt tcacgccgag tggcgcggtc acgttcaagg tcgatcacga cgcgaagacc
    11161 atcaccgcaa ccgtgaagct ggcgttctac aaccgcagct gcggcgtggg ccagtcctgc
    11221 gacgcgccgg tgggggacgt ggcccggatc gtgcgcgcga tcgaggccat gtggaacacc
    11281 ggtcagaagg tgaagtgcta cacgttctcc gtcgtcgtcg atggccggac cgtctccagt
    11341 caggcggatg ccggccggaa tgaggtcgac gtcgggctgg actacggccc ggtgccggtc
    11401 cgcgccttcg tccacggcga gtacaacgtc gcgacgacga ccgacccgct gagcggttcg
    11461 cccgacaacc ggatcgaccc ggtccacgac ccggcctcgc cgacgacgtg gccagggagg
    11521 acgtacgacc agacctacgc gcacgagttc ggccacatcc tcggcctcga cgacaactac
    11581 gaccccgcgc ggccggggtt cccggttccg gggacgtccg acgacctcat gttccgcaag
    11641 cagggcgacg tcaccgcaga gatggtcaag cgcgtcgtcg agcgcagcgg ccaggtcgag
    11701 ctcgaggacc tggagtgtgg ctggaccacc aacgccgaca cgccgctggg cactgtccgg
    11761 ggcacgcatt gcgagggcct cgggggtgag tggacgatca acggtgacga gaagctcctg
    11821 agccgcggca cggtgacgtc gctgtggaac gtcaccatcg accccacgac cctggcgggc
    11881 aagttccgcc acgagaagat ccaggacatc aagccgacga tcacaaccag caaccagaag
    11941 gggtccgcgc gcatcgtgaa gggccaggat ggccgcgtca cgatgac
//
LOCUS       DBNHXO010000505         1876 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2346515, whole genome shotgun sequence.
ACCESSION   DBNHXO010000505 DBNHXO010000000
VERSION     DBNHXO010000505.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2346515"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="AC162A_10180"
     CDS             <1..246
                     /locus_tag="AC162A_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011254.1"
                     /translation="VGAAADPVYPGSRVFRQFETAPQKWIRFRVLDPVVEVRVMVVAL
                     GKHGNEVSRSRIAIVTTGGDVTGEGESPPPDVPPDSP"
     gene            308..1243
                     /locus_tag="AC162A_10185"
     CDS             308..1243
                     /locus_tag="AC162A_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014874209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5011255.1"
                     /translation="MDHDQANPSRESRPEATRTIEGIGDAFRARGYIAERSLATTVFL
                     ALELGRPLLLEGEAGVGKTELAKVLAATLGSRLIRLQCYEGLDVNTAVYEWNYPRQML
                     EIRLLEARGEIDKASAHDIFGPDFLLKRPLLQALEATDGNAPVLLIDEIDRADEEFEA
                     YLLEILSDFQVTVPEIGTIRAVQAPRVVLTSNRTREVHDALKRRCLYHWIDYPTATKE
                     FEIVATRVPDAPPRLAREVVSFVHRLRAADLTKVPGVAETLDWAAALLSLGAAELSPD
                     VVDETLGVVLKYEEDIRQVRGSVARQYLAEATAQA"
     gene            1248..>1876
                     /locus_tag="AC162A_10190"
     CDS             1248..>1876
                     /locus_tag="AC162A_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011256.1"
                     /translation="MSDDASPILRAPTGELIDGRRLLAEAVGFGRALRAAGLSVDMAA
                     SIDFARALGLVDIGSKGIVRDAGAALFVRRRDDREVYDRVFAQFWRRRGRKLPTLRME
                     GLIPDAERDEDGEPGAADEGGEGREATMSELATMGVQVATDADEGAEIEGVTISPDAY
                     SKGEVLRHRDFDRMTAAELRDAERFVDLLRPRLERRRTRRFELHPHGRRV"
BASE COUNT          263 a          666 c          691 g          256 t
ORIGIN      
        1 gtcggagccg cggcggatcc ggtgtacccg ggatcgcgcg tcttccggca gttcgagacc
       61 gccccgcaga agtggatccg gttccgcgtg ctggacccgg tcgtcgaggt ccgggtcatg
      121 gtcgttgccc tcggcaagca cgggaacgag gtgtcgcgca gccggatcgc gatcgtgacg
      181 accggcggcg acgtgacggg agagggcgaa agcccaccac ccgacgttcc ccccgacagc
      241 ccctgacgtc ccggcgtcgg cgacccgcac gcgctcgggc accgtccccg gtgtaggatc
      301 gccgcccatg gatcacgatc aggcgaatcc ctcccgcgag tcccggcccg aggcgacccg
      361 gacgatcgaa ggcatcgggg acgcgttccg cgcccggggc tacatcgccg agcggtcgct
      421 cgcgacgacg gtgttcctcg cgctcgagct cggccgcccg ctgctcctcg agggtgaggc
      481 tggcgtcggc aagaccgagc tcgccaaggt cctcgcggcg accctcggct cgcggctgat
      541 ccggctccag tgctacgagg gactggacgt caacacggcg gtctacgagt ggaactaccc
      601 gcgccagatg ctcgagatcc ggctgctcga ggcgcgcggc gagatcgaca aggcctcggc
      661 gcacgacatc ttcggtcccg acttcctgct caagcggccg ctgctccagg ccctcgaggc
      721 cacggacggc aacgcgccgg tcctgctgat cgacgagatc gaccgcgccg acgaggagtt
      781 cgaggcctac ctgctcgaga tcctgtccga cttccaggtc acggtgcccg agatcggcac
      841 gatccgggcg gtccaggcgc cgcgtgtcgt cctcacgtcg aatcggaccc gggaggtcca
      901 cgacgcgctc aagcggcgct gcctgtacca ctggatcgac tacccgacgg cgaccaagga
      961 gttcgagatc gtggccacgc gcgtgccgga cgcgccgccg cgcctcgctc gcgaggtggt
     1021 gagcttcgtc catcggctgc gtgccgcgga cctgaccaag gtgccgggcg tcgccgagac
     1081 gctcgactgg gcggccgccc tgctgtcgct cggcgccgcg gagctgtcgc cggacgtcgt
     1141 cgacgagacc ctgggcgtgg tcctcaagta cgaggaggac atccggcagg tgcgcgggtc
     1201 cgtcgcccgc cagtacctcg ccgaggccac cgcccaggcc tgacgcgatg agcgacgacg
     1261 cgtccccgat cctgcgtgcc ccgacgggcg agctgatcga cgggcgccgc ctgctcgccg
     1321 aggcggtcgg gttcggacgc gcgctgcggg ccgccggcct gtcggtcgac atggccgcct
     1381 cgatcgactt cgctcgggcg ctgggcctcg tggacatcgg cagcaagggg atcgtgcgcg
     1441 atgcgggggc cgccctgttc gtgcgtcgcc gcgacgaccg cgaggtctac gaccgcgtgt
     1501 tcgcccagtt ctggcgccgc cggggccgga agctgccgac gttgcgcatg gagggcctga
     1561 tccccgacgc cgagcgcgac gaggatggcg agccgggggc ggccgacgag ggcggcgagg
     1621 gtcgcgaggc cacgatgtcg gagctcgcga cgatgggcgt ccaggtcgcg acggatgccg
     1681 acgaaggggc ggagatcgag ggggtcacga tctcaccgga cgcctactcg aagggcgagg
     1741 tgctgcgcca ccgggacttc gaccggatga cggccgccga gctgcgcgac gcggagcggt
     1801 tcgtcgacct gctgcggccc cgtctcgagc gtcgcaggac ccggcggttc gagctccacc
     1861 cgcacggccg gcgggt
//
LOCUS       DBNHXO010000506         5524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1354892, whole genome shotgun sequence.
ACCESSION   DBNHXO010000506 DBNHXO010000000
VERSION     DBNHXO010000506.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1354892"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="AC162A_10195"
     CDS             complement(<1..389)
                     /locus_tag="AC162A_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR5011257.1"
                     /translation="MRHDQIALQLYTVRRLMALDLPGTLRAVAETGYRSVELAGLPDL
                     APDELARLLADAGLTAISSHEGIDRLRADPGAVADRVAAVGCSRVIVPWMPPDDRRTL
                     DDVRRFAAELGRIAETLAARDITLGYH"
     gene            462..1562
                     /locus_tag="AC162A_10200"
     CDS             462..1562
                     /locus_tag="AC162A_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5011258.1"
                     /translation="MEPLGIGIVGTGNIAGGYARDIPTHPHIRLVAATDVDPARSAAF
                     GEAHGCRIHASLDGLLADPAVDIVVNLTVHHAHVEVSKRALEAGRHVYSEKPMALTSS
                     EARALIATAEDHGVRLGCAPATFLGEAQQTAAAWIRDGRLGTIRAVYADVSHGRIETW
                     HPSPEPFFDVGVLVDVAVYPLTLVTTMIGRATSVRAWGWDLVADRVTVAGTPFRIGSP
                     DLIIVAVELDGGAVLRLTGSFYVGRPAKHRGGLEFHGDAASLALGNFQDFNAAVEVGP
                     YGGDYAPVPYVREPFAGIAWGRGVADLAAAISEARPHRASAEQAAHVVDVLEAAQASM
                     ADDGRRIEVTSTFPPPPLMPWATLPEAESVGG"
     gene            1555..1893
                     /locus_tag="AC162A_10205"
     CDS             1555..1893
                     /locus_tag="AC162A_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011259.1"
                     /translation="MADDGRTVVLRHSRDKDGSRYLGATVAAGTLRIEGQDLGPGVEA
                     ILGAREYEWTWTVAAAAIPAAVDVLGGAPGSDPLTVLAAWSSANGDADPGQRLRDAGL
                     AVEFWSRIGD"
     gene            complement(1858..2382)
                     /locus_tag="AC162A_10210"
     CDS             complement(1858..2382)
                     /locus_tag="AC162A_10210"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006126628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5011260.1"
                     /translation="MRTPHLRTGRLVLDPLRVADADAMVTVLGDPALYAFTGGEPPEP
                     DALRETYGHLVAGPSDDPAEAWHNWVVRLAATGTAVGTVQATLWEGERRAAVAWLTGT
                     PWQGHGYASEAARAVVDWLASVGVRTIEAHIHPDHRASAAVAVRIGLRATEEVVDGEH
                     TWRSVADATPELDG"
     gene            complement(2392..3633)
                     /locus_tag="AC162A_10215"
     CDS             complement(2392..3633)
                     /locus_tag="AC162A_10215"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR5011261.1"
                     /translation="MTHFALDDPLLDAQALRVAGSAVYGGADPEECLAACARVRDTDL
                     ISWYVAWMAAADAAEAIGNREADEGHGESARGAFLRASNYARNAGVMLLGAPMDSRLV
                     EAQARQTALFRRAASLLARPPELVEIPFEATTLPGYFFSAGEGRRETMILTGGYDGTA
                     EELYLFNGAAALERGYNVLAFDGPGQGAALLERGLTMRHDWESVVTPVVDFLVGRDDV
                     DPERIALIGLSLGAHLAPRAASAEHRLAACIADCGSFDLYAAFLARVPRPLAGPFDRG
                     RRPAVAVMRRILERLAKDPTAGWVLRRGMLVHGVDDVIDYVEALRPFTLEGRAGAIRC
                     PTWVCSAEGDDISASAPQLVDALTCQHEYVHFREAEGAGDHCEVNARALYHRESFGWL
                     DLVLHPERADPDEAPRSFEPA"
     gene            3776..4672
                     /locus_tag="AC162A_10220"
     CDS             3776..4672
                     /locus_tag="AC162A_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-methyltransferase"
                     /protein_id="HIR5011262.1"
                     /translation="MVDDILEGNRGRVVLADNLVTLAALPDDSIDLSYNDPPFNTGKR
                     QSLRRLRTIRDEVDGDRTGWAGRRYRTETLGVQGYDDVHEDYLGFLEPRLAEIRRVLK
                     PTGSLYLHLDAREVHYVKVMLDGIFGRECFLNEVIWAYDYGARTSRRWPPKHDTILVY
                     VKDPGRYAFDAEAVDRIAYMAPDLVGPEKAARGKLPTDTWWHTIVPTGGRERTGYPSQ
                     KPVGIVRRMIQASCPPNGIVADFFAGSGSAGAAAYELGRRFLMVDDNPAACAIMRDRF
                     AAFDGVAYAGFDTIDMPEPAGA"
     gene            4683..5120
                     /locus_tag="AC162A_10225"
     CDS             4683..5120
                     /locus_tag="AC162A_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR5011263.1"
                     /translation="MPLSGDYAPGTARWARDQAERYEKSGGATAGDLRGRPIIVLTSV
                     GAKTGKLRKTALMRVEHDGVYAVVASLGGAPRHPVWYYNLRKQPHVELQDGEATLDYR
                     AREVTGDEKATWWARAVEAWPDYAKYQARTERQIPVFVLEPMA"
     gene            complement(5138..>5524)
                     /locus_tag="AC162A_10230"
     CDS             complement(5138..>5524)
                     /locus_tag="AC162A_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011264.1"
                     /translation="GGALAQGYATDDFPPIDEPIRLFASALAGDGPTPWGFVPLSVPE
                     DHRPFVQPLRQAYDLRAPVRAIFAEQQVPAPEWPHTCAFALVVELARVREAIDHRIAV
                     RLVLETVNGMAKTAPMTAKHLRDAAG"
BASE COUNT          800 a         1966 c         2023 g          735 t
ORIGIN      
        1 ttgtgatagc cgagcgtgat gtccctggcg gccagcgtct cggcgatacg cccgagctcg
       61 gcggcgaagc gccggacgtc atcgagcgtc cgccggtcgt cgggtggcat ccagggcacg
      121 atcacgcgcg agcacccgac ggcagcgacc cggtcggcga cggcaccggg gtcggcccgc
      181 agccggtcga tcccctcgtg ggacgagatg gccgtcaggc cggcatcggc cagcagccgc
      241 gcgagctcgt cgggtgcgag atcgggcagg cccgcgagct cgaccgagcg atagccagtc
      301 tcggcgaccg cgcgcagcgt gccggggagg tcgagggcca tgagccggcg gacggtgtac
      361 agctggagcg cgatctggtc gtgacgcatg ccctgacggt acggcatcgc ccgtgcgcga
      421 tggagccccg cgtgcgcacc cgcatcgcgt aggctcgggg catggagccc ctggggatcg
      481 ggatcgtcgg caccggcaac atcgccggcg gctacgcccg ggacatcccg acccacccgc
      541 acatccgcct cgttgcagcg accgacgtcg atcccgcccg ttccgcggcg ttcggagagg
      601 cccacggctg ccggatccac gcctcgctcg acggcctgct tgccgatccg gccgtcgaca
      661 tcgtggtcaa cctcacggtc catcacgcgc acgtcgaggt ctcgaaacgc gcgctcgagg
      721 ccggccgcca cgtctacagc gagaagccga tggcgctcac ctcctcggag gccagggcac
      781 tcatcgcgac cgccgaggac cacggggtgc gtctcgggtg cgcacccgcg acgttcctcg
      841 gcgaggcgca gcagaccgcg gcggcctgga tccgagacgg ccgcctgggc acgatccggg
      901 ccgtgtacgc cgacgtgagc cacgggcgga tcgagacctg gcacccgtcg cccgagccgt
      961 tcttcgacgt cggcgtgctg gtcgacgtcg ccgtgtatcc actgaccctg gtcacgacga
     1021 tgatcggccg cgcgacgtcg gtcagggcgt ggggctggga cctcgtcgcg gatcgcgtca
     1081 cggtcgccgg gacgccgttc cgcatcggga gcccggacct catcatcgtc gccgtcgaac
     1141 tcgacggcgg ggccgtcctc cggctgaccg ggagcttcta tgtcggccgg ccggccaagc
     1201 atcggggcgg gctcgagttc cacggcgatg ccgcatccct cgcgctgggc aacttccagg
     1261 acttcaacgc ggcggtcgag gtcgggccat atggcggcga ctacgcgccg gtcccgtacg
     1321 tccgcgagcc gttcgcgggc atcgcctggg ggcgcggcgt ggccgacctc gcggccgcga
     1381 tctccgaggc tcgcccgcat cgagcctcgg ccgagcaggc cgcccacgtc gtcgacgtcc
     1441 tcgaggcggc ccaggcgtcg atggcggacg acgggcgccg catcgaggtc acctcgacgt
     1501 tccccccgcc tccactgatg ccatgggcaa cgctcccgga ggccgagtcg gtcggtggct
     1561 gacgacgggc ggaccgtcgt gctgcgccac tccagggaca aggacggtag tcgctacctc
     1621 ggggcgaccg tggccgcagg cacgctgcgg atcgagggcc aggacctggg acccggcgtc
     1681 gaggcgatcc tcggtgcccg cgagtacgag tggacgtgga cggttgcggc ggccgccatt
     1741 ccggccgccg tggatgtgct gggcggcgcg ccgggatccg atcccctgac cgtgctcgcg
     1801 gcgtggtcga gcgcgaacgg ggacgccgac ccgggtcagc ggctgcggga cgccgggcta
     1861 gccgtcgagt tctggagtcg catcggcgac tgaccgccag gtgtgctcgc cgtcgacgac
     1921 ctcctcggtc gcccgcaggc cgatccgtac cgcgactgcc gccgacgctc ggtgatcagg
     1981 gtggatgtgc gcctcgatcg ttcggacgcc cacggacgca agccagtcga ccaccgcccg
     2041 ggcggcctcg gaggcgtacc cgtgtccctg ccacggcgtc ccggtcagcc atgccaccgc
     2101 cgcccgccgc tcgccctccc agagcgtcgc ctgcaccgtc ccgacggccg tgccggtggc
     2161 cgcgagccgg acgacccagt tgtgccaggc ctcggcgggg tcgtcggacg gacccgcgac
     2221 gagatgcccg taggtctcgc gcagcgcgtc gggctccggg ggctcgccgc cggtgaacgc
     2281 atacagcgcc gggtcaccca ggacggtgac catcgcgtcc gcgtcggcca cgcggagtgg
     2341 atccaggacg aggcgtcccg tgcgcaggtg tggcgtccgc acgggtcgcc gtcaggccgg
     2401 ctcgaaggag cgtggcgcct cgtcagggtc cgctcgctcg ggatggagga cgaggtcgag
     2461 ccagccgaag gactcgcggt ggtacagggc gcgtgcgttg acctcgcagt ggtcgccggc
     2521 tccctcggct tcccggaagt ggacgtactc gtgctggcag gtcagggcat cgacgagctg
     2581 gggcgccgac gcgctgatgt cgtcaccctc ggcgctgcac acccaggtgg ggcagcggat
     2641 cgcgccggcg cgcccttcca aggtgaacgg ccgcagggcc tcgacgtaat cgatgacgtc
     2701 gtcgacgccg tggacgagca tcccgcggcg cagcacccag ccggcggtgg gatccttcgc
     2761 aaggcgttcg aggatccggc gcatgaccgc gacggcgggc cgccggccac ggtcgaacgg
     2821 gccggcgagc gggcgtggaa cccgggccag gaacgccgca tagaggtcga acgacccgca
     2881 gtcggcgatg cacgcagcga gccggtgctc ggcgctcgcc gcccgtggcg cgaggtgggc
     2941 acccaggctg agcccgatga gcgcgatgcg ctccgggtcg acgtcgtcgc gcccgacgag
     3001 gaagtcgacg acgggggtga ccacggactc ccagtcgtgc cgcatcgtca ggccccgctc
     3061 gagcagggcg gcgccctgcc ccggtccgtc gaaggccagg acgttgtagc cccgctcgag
     3121 ggcggccgcg ccgttgaaca ggtacagctc ctcggcggtc ccgtcgtacc caccggtgag
     3181 gatcatggtc tcgcgacgac cctcgccggc gctgaagaag tagcccggca gcgtggtcgc
     3241 ctcgaacggg atctcgacca gctccggcgg gcgggcgagc aacgatgcgg cgcgccggaa
     3301 cagcgccgtc tgccgcgcct gggcctcgac gagccgcgag tccatcggcg ccccgagcag
     3361 catcacgccg gcgtttcgcg cgtagttcga cgcgcgcagg aaggcgccac gcgcggactc
     3421 cccgtgaccc tcgtcggcct cccggttccc gatcgcctcc gcggcgtcgg ctgcggccat
     3481 ccaggcgacg taccagctga tgaggtccgt gtcgcggacg cgggcgcacg ctgcgaggca
     3541 ctcttccgga tcggcgccgc catagaccgc cgaaccggcc acgcgcagcg cctgggcgtc
     3601 caacagcgga tcgtcgagcg cgaagtgcgt catcgtcggt caggatacgt gcggacctcc
     3661 cgatcgcgcc ctgcgggcca gactggacgc gagcggctat cgtcaacgga tgaccggggt
     3721 gctccgcccg tcggggcacg cgccgtcggg aggcgaggcg cgctaggctg gccgcgtggt
     3781 ggacgacatc ctcgaaggca accgcgggcg ggtcgtcctc gccgacaacc tcgtcaccct
     3841 ggcggccctc cccgacgact cgatcgacct ctcgtacaac gacccgccgt tcaacaccgg
     3901 caagcgccag tcgctccggc ggctgcggac gatccgggac gaggtcgatg gcgaccggac
     3961 gggctgggcc ggcaggcggt atcggaccga gacgctgggc gtgcaaggct atgacgacgt
     4021 ccacgaggac tatctcgggt tcctcgagcc gcgcctggcg gagatccgcc gggtcctgaa
     4081 gccgacggga agcctgtacc tccacctcga cgcgcgcgag gtccactacg tgaaggtcat
     4141 gctggacggc atcttcggcc gggagtgctt cctcaacgag gtcatctggg cctacgacta
     4201 cggggcccgc acgtcgcggc gctggccacc caagcacgac acgatcctcg tctacgtgaa
     4261 agaccctggc cgctatgcct tcgatgccga ggccgtcgac aggatcgcgt acatggcgcc
     4321 cgacctcgtc ggccccgaga aggcggcgcg cggcaagctc ccgacggaca cctggtggca
     4381 cacgatcgtg ccaacgggcg gtcgcgagcg gaccggctat cccagccaga agcccgtcgg
     4441 catcgtgcgc cgcatgatcc aggcctcgtg ccccccgaac ggcatcgtcg ccgacttctt
     4501 cgccggcagc gggtcggccg gggcggcggc gtacgagctg gggcgccggt tcctgatggt
     4561 cgacgacaat ccggcggcct gcgcgatcat gcgcgaccgt tttgcggcgt tcgatggcgt
     4621 cgcttacgcc ggcttcgaca cgatcgacat gccggagccg gccggagcct aggagccatg
     4681 ccatgccgct gagcggagac tacgcacccg ggaccgcgcg ctgggcgcgc gatcaggcgg
     4741 agcgctatga gaagtcaggc ggcgcaacgg ccggggacct ccgcggtcgg ccgatcatcg
     4801 tcctgacgtc ggtgggcgcg aagacgggca agctgcgcaa gaccgccctg atgcgcgtcg
     4861 aacacgacgg cgtctatgcc gtcgtggcgt cgctgggtgg cgcgccgagg catcccgtct
     4921 ggtactacaa cctgcgcaag caaccccacg tggagctcca ggacggcgag gcgacgctgg
     4981 actaccgcgc gcgcgaggtc accggcgacg agaaggcgac ttggtgggcg cgggccgtcg
     5041 aggcgtggcc cgactacgcc aagtaccaag ccaggacgga gcgccagatc ccggtgttcg
     5101 tgctggagcc aatggcgtag gttcgccgat ggccgtccta cccggcggcg tcgcgcagat
     5161 gcttcgcggt catcggcgcg gtcttggcca tcccattgac ggtctcgagg acgagccgca
     5221 cggcgatccg gtggtcgatc gcctcgcgga cgcgggcgag ctcgaccaca agcgcgaagg
     5281 cacaggtgtg cggccactcg ggcgccggga cctgctgctc cgcgaagatc gcacggaccg
     5341 gagcgcgaag gtcgtacgcc tggcggagcg gctgcacaaa cggccgatgg tcctcgggca
     5401 cgctgagcgg aacgaagccc cacggcgtcg gaccgtcgcc ggcgagcgcc gaggcgaaca
     5461 gccggatcgg ctcgtcgatc ggggggaaat cgtcggtcgc gtagccctgg gccagcgcgc
     5521 ctcc
//
LOCUS       DBNHXO010000507         2503 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2942277, whole genome shotgun sequence.
ACCESSION   DBNHXO010000507 DBNHXO010000000
VERSION     DBNHXO010000507.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2942277"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..99)
                     /locus_tag="AC162A_10235"
     tRNA            complement(15..99)
                     /locus_tag="AC162A_10235"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63..65),aa:Tyr,seq:gta)
     gene            complement(104..179)
                     /locus_tag="AC162A_10240"
     tRNA            complement(104..179)
                     /locus_tag="AC162A_10240"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Ala,seq:cgc)
     gene            272..1168
                     /locus_tag="AC162A_10245"
     CDS             272..1168
                     /locus_tag="AC162A_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR5011265.1"
                     /translation="MSAGLALGLLASLAWGFVDVAAALAGRRLGSLRVFAGAQVVSLA
                     TLALIVFASGGLGQDPLAGVLAGLPWGVGAAAAYLCYFTALRIGPLTVVSPVIVAYGG
                     TTVLLAVVLRGETLLPAQAVGAALATAGVVLACVVFDGGTMGGARIVGLGVVFALATM
                     VLFAVVTVGLAAPIEDHGWLPVVVGSRLANTGVALLLLAVVRRSSSRRLDVLTQPAGL
                     LDRASLGLVIVAGVFDIAAFAVYAVGLEVAPTWLIGLASSFGPVIVVVYAVWRLGERP
                     RSTQWAGLALLAAGVMVLALAG"
     gene            1468..>2503
                     /locus_tag="AC162A_10250"
     CDS             1468..>2503
                     /locus_tag="AC162A_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR5011266.1"
                     /translation="MATASDVSEALATLQARWGAAAPRRGGELGLAVEGALALAPLPL
                     DDPANADRPGLIPLAPVVAPRPAQPAADVDGRVVPTGFAALDAILGMGGLPRSATIAL
                     RGDASSGKTTLALRLAAEAQATGAIVAWLDLARAFDPVEAVARGIRPEWLVVLTPVDL
                     EEALALSAALLAARTVDLLVVDLPDGRDPAVSGVRVGDRLGRLAALARRAGTLLVVVE
                     PNALGRGLAAAVEEASGLRLELERTGWIRLGRDVVGQRSAVTVARNHHGPPGRRADLE
                     ILYAEGGARDACLERSGLLGPPSTSFDLKGPPPSADTHATPAPALAPPAAPARPVPPR
                     FSGERRHLRLV"
BASE COUNT          263 a          872 c          960 g          408 t
ORIGIN      
        1 gtctccaggg atgctggtgg gccggggcgg aatcgaaccg ccacagccga aggcgaaggt
       61 tttacagacc tttgggctca ccacctgccc aaccgaccca cgatggtgga gatgagggga
      121 ctcgaacccc tggcctccgc gatgcgaacg cggcgctctc ccagctgagc tacatcccca
      181 ctggggtcac ccggcagacc gggtggaccg gccgggaagt gtagcacgcg acatcggcca
      241 ttccgcctcg gcggacccgt agactccgcc gatgtccgcg ggtctcgcgc tcggcctcct
      301 ggcatcgctc gcgtggggat tcgtggacgt cgcggcggcc ctcgccggtc ggcgcctcgg
      361 cagcctgcgc gtgttcgccg gggcgcaggt cgtgagcctc gcgacactcg cgctgatcgt
      421 gttcgcgagc ggcgggctcg gccaggatcc acttgccggc gtgctggcgg gactcccctg
      481 gggcgtcgga gcggccgcgg catacctgtg ttacttcacc gcgcttcgga tcgggccgct
      541 caccgtggtc agccccgtga tcgtcgccta cggcgggacg acggtgctgc tggcggtggt
      601 gctgcggggc gagaccctgc tgccggcaca ggccgtcggg gcggcgctcg cgacggcggg
      661 tgtggtgctg gcctgcgtcg tgttcgacgg cggcacgatg ggcggggcgc ggatcgtcgg
      721 cctgggcgtc gtgttcgcgc tggccacgat ggtgctgttc gcggtggtga cggtcgggct
      781 cgctgcgccg atcgaggacc atggctggct gccggtggtc gtggggtcga ggctggccaa
      841 cacgggcgtg gcgctgctgt tgctggccgt ggtccgccgt tcgtcctcgc gacggctgga
      901 cgtcctgacg cagccggctg ggttgctgga tcgggcctcg ctggggctcg tgatcgtggc
      961 gggcgtgttc gacatcgcgg cgttcgccgt ctatgcggtg ggcctggagg tggcgccgac
     1021 atggctgatc ggcctcgcaa gctcgttcgg tccggtgatc gtggtcgtgt acgcggtgtg
     1081 gcgactgggc gagcgaccgc gttcgacgca gtgggcgggc ctggcgctgc tggcggcggg
     1141 cgtcatggtg ctggcgctgg cggggtaggc atttcctcgg cccgccctcc cctcgggccg
     1201 gccgtgatcg cctcatgtgc aatttgggcc ttggcgggcc acgcacggtc ggatcgtgac
     1261 cagtggccgg gcgagtccgg cccttgtcgt cgatgctgat ttccgcatgt gcagcgccgg
     1321 ggacaagagg ccaagacgct cggtcttcgg cgcgcgcagg cgccagattg cacatgtcag
     1381 gagcacgcgg ctcgcctggc gggagcggtg cgtggggccg ttgtcctctt gcatgaatag
     1441 aacagatgtt ctagaattcg acgtccgatg gcgaccgcat ccgacgtctc cgaggccctc
     1501 gcgacgctcc aggctcgctg gggtgccgca gctccgcggc ggggcggcga gctgggcctg
     1561 gcggtcgagg gtgcgctcgc gctcgcgccg ctgccgctgg acgacccggc caatgccgac
     1621 cgacccggac tgatcccgct ggcaccggtc gtcgcgcctc gcccggccca gccggcggcg
     1681 gatgtggacg gccgcgtcgt gcccacgggg ttcgccgccc tcgacgccat tctcggcatg
     1741 ggcgggctgc cgcgctccgc caccatcgcg ctgcgcgggg acgcctcgtc gggcaagacc
     1801 accctcgccc tgcggctcgc ggcggaggcg caggcgaccg gcgcgatcgt cgcctggctg
     1861 gacctcgccc gggccttcga tcccgtggag gccgtcgccc gcgggatccg cccggaatgg
     1921 ctcgtggtgc tcacccccgt cgacctcgag gaggccctcg cgctctcggc tgcgctcctc
     1981 gcggcccgga ccgtggacct cctcgtcgtc gatctgcccg acggccggga cccggcggtc
     2041 agcggcgtgc gtgtgggcga ccggctgggc cggctcgcgg cgctcgcgag gcgcgcgggg
     2101 acgctgctcg tggtcgtgga gccgaacgcg ctcgggcggg gcctggcggc cgccgtcgag
     2161 gaggcgagcg gcctgcgcct cgagctggag cggaccggct ggatccggct gggccgggac
     2221 gtcgtggggc agcgcagcgc ggtgacggtc gcgcgcaacc atcatgggcc gccgggacgg
     2281 cgcgcggacc tcgagatcct gtacgcggag ggcggcgcgc gtgatgcctg cctggaacgt
     2341 tcgggcctcc tcggcccacc gtccacctcc ttcgacctga aaggcccccc tccatcggcc
     2401 gatacccatg cgactcctgc acctgctctg gccccacctg ccgctccggc tcgcccggtc
     2461 ccgcctcggt tctccgggga gcgacggcat ctccgcctcg tgg
//
LOCUS       DBNHXO010000508         2674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_366133, whole genome shotgun sequence.
ACCESSION   DBNHXO010000508 DBNHXO010000000
VERSION     DBNHXO010000508.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_366133"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..697)
                     /locus_tag="AC162A_10255"
     CDS             complement(206..697)
                     /locus_tag="AC162A_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011267.1"
                     /translation="MSASTPTASSTFWTTTQPQRGVASRLTAGFLFITTGVVLASAVL
                     GLSVASAIIARGIVVADAGDVATIRALTPLFALFGVIAAAHLVAGFGMAFGSRQAASL
                     GIGLGAFNVVAGVVGLIVAATGSRNQADGVGIAMTFVAMGIVLAVAARAADWNTHGPV
                     DAD"
     gene            complement(839..1417)
                     /locus_tag="AC162A_10260"
     CDS             complement(839..1417)
                     /locus_tag="AC162A_10260"
                     /inference="COORDINATES: protein motif:HMM:NF016955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HIR5011268.1"
                     /translation="MSFLTRTLGLDNPASPELPVDPHAPLPSASEADTASVKLIALQL
                     SAMPPARARYLAAFAYLLGRAADANMSISDDETAEMVKLMEDAGGLDPETARLVVVLA
                     TAQADSYGATEDFLVTREFKAISTVEEREQLLRCCFLVMAADDEIDETESWLANRLAE
                     ELDIERPDLNEIRNEFYDQLSSVREIRRFAAN"
     gene            complement(1455..1775)
                     /locus_tag="AC162A_10265"
     CDS             complement(1455..1775)
                     /locus_tag="AC162A_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR5011269.1"
                     /translation="MTEIPDDVEIETIWVVEVPYTPEAEVRRPQFRRAHLTRIARLIR
                     EGRVVEAGGTADLTKAVLLIRAETEAEVLALIEEDVYTSGGVWHSPIARAFGRVIPRE
                     ATSG"
     gene            1816..2523
                     /locus_tag="AC162A_10270"
     CDS             1816..2523
                     /locus_tag="AC162A_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="HIR5011270.1"
                     /translation="MPERTIPILPCRSIDATLAFYGALGFEVTYRQERPNTYAAIARG
                     AIELQFFVLKALEPEANWSTCYVIVDDVDRLYEAMTDGLRAATGRVPGHGYPRIGALR
                     DMAYGVRQFVVVDPTGNHIRIGQPIEARPTGTVATMSRLERSLGAAVTLADSKGDPSA
                     AASALDSAFRADPPIAGGVAYRALVLRADLAHRLDHPDEARAWLIQASGVAVPEADEG
                     TTADERRRASELAEALG"
BASE COUNT          382 a          921 c          981 g          390 t
ORIGIN      
        1 cgggtcgtcg tgcacgtgcc ggcccccggc ggctcggcgc tgccgatgga gctgcggggc
       61 gtcgcctacg acgccgagct gctggccgag ctccgtcggc gggtcggcga gggggtcatc
      121 gacctcagcc tgggctgagc ccagcgccgc gggcctttcg cgcccttacc ggccgctgcc
      181 gcagccggcg agtcgccgtc gaggttcagt ccgcgtcgac cgggccatgc gtgttccagt
      241 cggccgcgcg ggccgcgacc gcgagcacga tgcccatcgc gacgaaggtc atcgcgatgc
      301 ccacgccgtc cgcctggttc cgcgagccgg tggccgcgac gatcaggccg accacgccgg
      361 cgacgacgtt gaaggcaccg agcccgatcc cgagcgaggc ggcctggcgg ctgccgaacg
      421 ccatcccgaa cccggccacg aggtgggccg cggcgatgac gccgaacagt gcgaacaacg
      481 gggtcagggc gcggatggtg gcgacgtcac cggcgtctgc gacgacgatg ccccgggcga
      541 tgatcgcgct ggcgacgctc aggccgagca cggccgaggc gagcacgacg cccgtcgtga
      601 tgaacaggaa acccgcggtc aggcgcgagg cgacgccgcg ctgcggctgg gtcgtcgtcc
      661 agaaggtcga ggacgcggta ggggtcgaag cggacatcgt gtgcggctct cccatgaccc
      721 ggtcactcga ccggcgttgc cgcgatcgtc gtcgcgcgcc agggagctcg ccatcggcgg
      781 ccgtcggcgg ccgccatcgt cattcgtcca tccccggccg gccgccgggg ctctgcgatc
      841 agttggcggc gaaccgccgg atctcgcgga cgctcgagag ctggtcgtag aactcgttgc
      901 ggatttcgtt gaggtcgggg cgctcgatgt cgagctcctc ggccaggcga ttggccagcc
      961 acgactccgt ctcgtcgatc tcgtcgtcgg ccgccatcac gaggaagcag cagcggagga
     1021 gctgctcgcg ctcctccacg gtggagatcg ccttgaactc gcgggtcacg aggaagtcct
     1081 cggtggcccc gtaggagtcg gcctgcgccg tcgccaggac gacgacgagc cgcgccgtct
     1141 ccgggtccag gccgccggcg tcctccatga gcttgaccat ctcggcggtc tcgtcgtcgc
     1201 tgatcgacat gttggcgtcg gccgcccgac ccaggaggta cgcgaaggcc gcgaggtatc
     1261 gggctcgcgc cggcggcatc gccgacagct gcagcgcgat cagcttgacg ctcgctgtgt
     1321 cggcctcgct cgcggagggc aacggggcgt gcgggtcgac gggcagttcg ggcgaggcgg
     1381 gattgtcgag gccgagcgtg cgcgtgagga agctcatcgg gactccgggg cgtggcgtgg
     1441 caatgggcgg gagtctaccc cgaggtcgcc tcccgcggga tcacgcgccc aaaggcgcga
     1501 gcgatgggcg agtgccaaac gccgcccgag gtgtagacgt cctcctcgat cagggccagc
     1561 acctcggcct cggtctcggc ccggatcaaa aggaccgcct tggtcaagtc ggccgtgccg
     1621 ccggcctcga cgacccgtcc ctcgcggata aggcgcgcga tccgcgtcag gtgggcccga
     1681 cgaaactgcg gtcggcgcac ctccgcctcg ggcgtatacg ggacctcgac gacccagatc
     1741 gtctcgatct cgacgtcgtc ggggatctcg gtcatcgatc ggagctcctc gtgcttgggg
     1801 tggcagaatc gtacgatgcc tgaacggacg atcccgatcc tcccgtgtcg gtcgatcgac
     1861 gcgaccctcg cgttctacgg ggcgctcggg ttcgaggtga cctaccgcca ggagcgcccg
     1921 aacacctacg cggccatcgc ccgcggcgcg atcgagctgc agttcttcgt cctcaaggcg
     1981 ctggagcccg aggcgaactg gtcaacctgc tacgtgatcg tcgacgacgt ggatcggctg
     2041 tacgaggcga tgacggacgg gctgcgcgcg gcgaccggcc gggttcctgg tcacgggtat
     2101 ccgcggatcg gcgcgttgcg cgacatggcg tacggcgtgc gccagttcgt cgtcgtcgac
     2161 ccgacaggga accacatccg gatcggccag ccaatcgaag cgcgcccgac cggcaccgtg
     2221 gcgacgatgt cccggctcga gcggtcgctc ggcgccgcgg tcacgctcgc cgactcgaag
     2281 ggcgatccgt cggcggccgc ctcggcgctc gactcggcgt tccgcgccga tccgccgatt
     2341 gcgggcgggg tcgcctaccg cgccctggtc cttcgggcgg acctcgcgca ccggctcgac
     2401 catcccgacg aggcgcgtgc ctggctcatc caggcttcgg gcgttgcggt cccggaggcc
     2461 gacgagggga cgacagccga cgagcggcga cgagccagcg agcttgcgga agcgctcggg
     2521 tagcgccgtc gcgggcctaa ccgcgcgggt agactgccat tccgcgcggt gcccgccgcg
     2581 atgggacgtg atgacgatgg gaggcaggcg tgatcgagta ctttgatcgc gagatcgcga
     2641 ccactctgga gaactttcag gatccgaagc agga
//
LOCUS       DBNHXO010000509         2501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1951157, whole genome shotgun sequence.
ACCESSION   DBNHXO010000509 DBNHXO010000000
VERSION     DBNHXO010000509.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1951157"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="AC162A_10275"
     CDS             complement(<1..346)
                     /locus_tag="AC162A_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011271.1"
                     /translation="MKQIWVCGGCKSVNQLRASRCYKCHVPRPAAGQELAAVDVPASH
                     EDIVQRAATPGSRYTTWPFALVAIALVVVCVALRLANVIGALGMVEVLVIGLAPPTPE
                     IERVAWLGIAWLV"
     gene            complement(457..747)
                     /locus_tag="AC162A_10280"
     CDS             complement(457..747)
                     /locus_tag="AC162A_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011272.1"
                     /translation="MGIGDARADDLTVIVEHDGSIWVDVGSRAVAVEAPDLERILDRL
                     AADGGTLRLLGGTGRADDGDEPPDHESDPAMGAASYVLALAHELGLEVISEA"
     gene            complement(770..2140)
                     /locus_tag="AC162A_10285"
     CDS             complement(770..2140)
                     /locus_tag="AC162A_10285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR5011273.1"
                     /translation="MSSEHGIGGDPEEALRLAERVIYAAEKAGAGEAEALVVAGESAL
                     TRFANSEIHQNVASAEAFINLRFVNGRRVGVASSGRIDEEGIGALVERAAAIAAHVEE
                     LEEWAGLPSADPPVTLHGGWSERTAHATPELRAEGARAVIAAADDAGVTAYGSFATEA
                     EAIAVANTKGIRAAERRSTSQLLTVTMGPDNGTGYAEQCAVDATVIDAAAIGREAAER
                     ARTSANPVDIAPGDYPVVLQDYAVVDLLDMLGYLGFSALAVQEDRSFFERGKQVASPL
                     VSITDDGHDPAGLPMGFDAEGVPKQRLGLLEKGVCCEVAYDAQTAARVGRHSTGHGLP
                     APNPYGPFPTNMVMAPGDASFEELVGGLDRGLLVTRFHYTNPVHGKRVIITGMTRDGT
                     FLVEGGKVVGPVRNLRFTMSYLDALANVEAVSRERRCIRGFLGGSVVPSVRLSSFSFT
                     GATAAG"
     gene            complement(2137..>2501)
                     /locus_tag="AC162A_10290"
     CDS             complement(2137..>2501)
                     /locus_tag="AC162A_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HIR5011274.1"
                     /translation="VNLLPRDGMSFDDIIADTDDGLMFETNRSWSIDDRRLNFQFATE
                     VCREIKGGKPGKLYRNGTYTGITYELWRSMDAIGDASSWRMWGTPNCGKGEPGQTGHV
                     GHGVSGGRFRNVRVGVMG"
BASE COUNT          349 a          886 c          904 g          362 t
ORIGIN      
        1 ccaccagcca ggcgatgccg agccacgcga cgcgctcgat ctcgggggtc ggcggcgcca
       61 ggccgatgac gaggacttcg accatgccga gggcgccgat gacgttcgcc agccgcagcg
      121 cgacacaaac gacgaccagt gcgatggcga ccagggcgaa gggccaggtg gtgtacctcg
      181 aacccggcgt tgctgcccgt tgcacgatgt cctcgtgcga ggccggcacg tcgaccgcgg
      241 caagctcctg gcccgcggcc ggccgaggga cgtggcactt gtagcagcgc gatgcgcgca
      301 gctggttgac cgacttgcag ccgccacaca cccagatctg cttcatcgac ccctcatttc
      361 gcggacgcgt ggtcccgggc gggatcgtac ggcccgggtg gcctccgagg caggacctgt
      421 tcgtgggggc agcgatccag agaacgggcg ggcgggtcag gcctcggaga tcacctcgag
      481 gccgagctcg tgggcgagcg ccaggacgta ggaggcggcg cccatcgcgg ggtcgctctc
      541 gtgatccggc ggttcatcac cgtcgtccgc gcggccggtc ccgcccagca ggcgaagggt
      601 gccgccgtcc gccgccaggc ggtcgaggat tcgctccagg tcgggcgcct cgacggccac
      661 ggcccgtgac cccacgtcga cccagatcga gccgtcgtgt tcgacgatga ccgtgaggtc
      721 atccgcgcgg gcgtcgccga tacccatcgc cgcgctgcca cgcgtcccgt cagcccgcgg
      781 ccgtcgcgcc ggtgaacgag aacgaagaca ggcgtactga gggcacgacg ctgccgccca
      841 ggaagccgcg gatgcagcgt cgttcgcggc tcacggcctc cacgttggcg agcgcgtcca
      901 ggtacgacat cgtgaaccgc aggttgcgca cgggtccgac gaccttgccg ccctccacga
      961 ggaacgtccc gtcgcgggtc atcccggtga tgatcacgcg cttgccgtgg accgggttcg
     1021 tgtagtggaa gcgggtgacg agcaggcctc gatcgagccc gccgaccagc tcctcgaacg
     1081 aggcgtcgcc cggggccatg accatgttgg tcgggaacgg gccgtagggg ttgggggccg
     1141 gaaggccgtg tccggtggag tggcggccga cccgggccgc ggtctgcgcg tcgtaggcaa
     1201 cctcgcagca gacgcccttc tcgagcaagc ccaggcgctg cttcggcacg ccctcggcat
     1261 cgaagcccat cgggaggccg gcggggtcgt ggccgtcgtc ggtgatgctg accagcggcg
     1321 aagcgacctg cttgccgcgc tcgaagaacg agcggtcctc ctgcacggcc agggccgaga
     1381 aaccgaggta gccgagcatg tcgagcaggt cgacgaccgc gtagtcctgg agcacgaccg
     1441 ggtagtcgcc gggggcgatg tcgacggggt tggcgctggt ccgggcgcgc tcggcggcct
     1501 cgcggccgat cgccgcggcg tcgatgaccg tcgcgtccac cgcgcattgc tcggcatagc
     1561 cggtgccgtt gtcggggccc atcgtcaccg tcagcagctg cgaagttgac cgtcgctcgg
     1621 ccgcgcggat ccccttggtg ttggcgacgg cgatcgcctc ggcctcggtc gcgaacgagc
     1681 cgtaggcggt gacgccggcg tcgtcggcgg cggcgatcac ggcccgcgcc ccctcggcgc
     1741 ggagctccgg ggtggcgtgc gcggtgcgct ccgaccaacc gccgtgcagc gtgacgggag
     1801 gatcggcgga cgggagcccg gcccactcct cgagctcctc gacgtgggca gcgatcgcgg
     1861 cggcgcgctc gaccagcgcg ccgatgccct cctcgtcgat ccggcccgac gacgcgacgc
     1921 ccacgcggcg gccgttgacg aaccgcaggt tgatgaacgc ctcggcgctg gcgacgttct
     1981 ggtggatctc ggagttggca aagcgcgtga gcgcggactc gccggcgacg accagggcct
     2041 cggcctcgcc ggccccggcc ttctcggcgg cgtagatgac gcgttcggcg agccgcagcg
     2101 cctcctcggg atcgcccccg atgccgtgct cgctgctcat cccatcaccc ccacccgcac
     2161 gttgcggaac cggccgcccg agacgccgtg cccgacgtgg cccgtctgcc ccggctcgcc
     2221 cttgccgcag ttcggcgtgc cccacatgcg ccagctcgac gcgtcgccga tggcgtccat
     2281 cgagcgccac agctcatagg tgataccggt gtaggtgccg ttccgataca gcttgcccgg
     2341 cttgccgccc ttgatctcgc ggcagacctc cgtcgcgaac tggaagttca ggcgccggtc
     2401 gtcgatcgac cagctgcggt tggtctcgaa catgagcccg tcgtccgtgt cggcgatgat
     2461 gtcgtcgaag ctcatcccgt cgcggggcag caggttgacg t
//
LOCUS       DBNHXO010000510         3062 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1557435, whole genome shotgun sequence.
ACCESSION   DBNHXO010000510 DBNHXO010000000
VERSION     DBNHXO010000510.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1557435"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1138)
                     /locus_tag="AC162A_10295"
     CDS             complement(59..1138)
                     /locus_tag="AC162A_10295"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR5011275.1"
                     /translation="MGGAEGPARVAVVGAGAWGTTLAAILAQREPVVLVCHSPETAAR
                     IRKDRRNEARLPGIDLPAGIRATHELSALAEATDLIVFAVPSAHLRSTASEAAPHIPP
                     SADLLSVVKGIERGTHLRMTDVIAEAGAIDRRRIAALSGPNLALEIARRLPASAVVAA
                     DDVDLAARIQERLGRRRFRLYVNRDVLGVELCGALKNVVAIAAGAAEELGFGDNGKAG
                     LMTRGVAEMTRLGIAAGANPLTFAGLAGIGDVIATCGSSLSRNHRLGVELAKGRTWAE
                     IEATLPGVAEGAYTVDAALALADELGVEMPIAREVHRALFEGKSAKRCLIDLLARESK
                     DELADYDRWAAQVGAQRGAADPAAG"
     gene            complement(1138..2721)
                     /gene="der"
                     /locus_tag="AC162A_10300"
     CDS             complement(1138..2721)
                     /gene="der"
                     /locus_tag="AC162A_10300"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR5011276.1"
                     /translation="MPARTRGLPVVAFVGRPNVGKSTLFNRVVGDSRAAIVEDRARTT
                     RDRLYGDAEWNGRRFVVVDTGGLEIDPGDPIEAQVQLQARLAISEADVIVFLVDAETG
                     PMPADQDAADLLRRTTKPVLVAANKADNEKRELDAAEFYAFGWEETYAISASHGRGVG
                     DLLDAVVWVLPPETEAETARKTREAEAEAWASDMAAGRIEPFSTGDDGAADGEDGTEA
                     AAGEDGAVPDDDSAESARWDAMMAAEGDEPPAIAFVGRPNVGKSSLLNSLLGETRTIV
                     SDIPGTTRDAIDTTIPWGRSEVVLIDTAGIRKRGKVASGPDADRYSTLRALKAISRAD
                     VAVLVLDASDGLTAQDAHVAGYVVEEGRGLVIAVNKWDIVEDKTHKSFDEFAANLRRE
                     LPFIDYAPVVSISAKTGQRVQKVLELAVEVWGERRRRIPTGELNRLVSAAVERTPPAM
                     VRGRRSKIRYATQAGVAPPTFVFFATDPTSIHFSYRRYLENRLRDAYGFDGTPIRLVF
                     RDQIRAKLPRHRSARTHKS"
     gene            complement(2725..>3062)
                     /locus_tag="AC162A_10305"
     CDS             complement(2725..>3062)
                     /locus_tag="AC162A_10305"
                     /inference="COORDINATES: protein motif:HMM:NF014456.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011277.1"
                     /translation="VVVGGRHSANTKELTRLCEIVGTAAIQIENARDLVDPAPFEGAK
                     VVGVTGGTSTPIEDLRDVAERIFEIAGTPEAIADAASLARGALDAAAHPAGRTTSLPA
                     HPVASTGAA"
BASE COUNT          381 a         1142 c         1101 g          438 t
ORIGIN      
        1 ggttggatcg ccgggcgttc gatctgccgg gccaaccgct ggactgctct cccggcgttc
       61 aacctgcggc ggggtcggcg gcgccacgct gcgcgccgac ctgcgccgcc cagcggtcgt
      121 agtcggcgag ctcgtccttg gactcgcggg ccaggaggtc gatcaggcag cgcttggcgc
      181 tcttgccctc gaacagcgcg cggtggacct cgcgggcgat cggcatctcc acgcccagct
      241 cgtcggccag ggcgagcgct gcatccactg tgtaggcgcc ctcggcaacg cccggcagcg
      301 tggcctcgat ctcggcccag gtccggccct tggccagctc gacacccagg cggtggttgc
      361 gcgatagcga cgagccgcag gtggcgatga cgtcaccgat gccggccagg cccgcgaagg
      421 tcaacgggtt ggcgccggcc gcgatcccga ggcgggtcat ctcggcgacg ccacgggtca
      481 tcagaccggc tttcccgttg tcgccgaagc ccagctcctc ggcggcgccg gcggcgatgg
      541 cgacgacgtt cttgagcgcc ccgcacaact cgacgccgag gacgtcacgg ttcacgtaca
      601 gccgaaaccg ccggcgtccc agccgctcct ggatgcgcgc cgcgaggtcg acgtcgtcgg
      661 cggcgacgac cgccgacgcc ggcagcctgc gcgcgatctc gagcgcgagg ttaggcccgg
      721 acagggcggc gatccggcgg cggtcgatcg cgccggcctc cgcgatgacg tcggtcatcc
      781 ggagatgggt gccgcgctcg atgcccttga cgaccgagag caggtccgcg ctgggcggga
      841 tgtggggcgc cgcctcgctc gccgtcgacc gcaggtgcgc cgacggcacg gcgaacacga
      901 tgagatccgt cgcctcggcc agcgccgaca gctcgtgcgt ggcacggatc ccggccggca
      961 ggtcgatgcc cggcaggcgc gcctcgttcc gccggtcctt gcggatccgt gccgcggtct
     1021 ccggagagtg gcacaccagc acgaccggct cgcgctgggc gaggatcgcg gcgagcgtcg
     1081 ttccccaggc gcccgcgccg acgaccgcga cccgcgcggg gccctcggcg ccgcccatca
     1141 gctcttgtgc gtgcgcgccg accggtggcg cggcagcttc gcgcggatct ggtcccggaa
     1201 cacgagccgg atcggcgtcc cgtcgaagcc ataggcgtcg cgcagccggt tctcgaggta
     1261 gcggcggtac gagaagtgga tcgacgtcgg atccgtcgcg aagaacacga acgtcggcgg
     1321 cgccacgccc gcctgggtcg cgtaccggat cttcgagcgt cgcccgcgga ccatcgccgg
     1381 cggcgtgcgc tcgacggccg ccgagaccag gcggttgagc tcgcccgtcg ggatccgccg
     1441 ccgccgttcg ccccacacct cgaccgcgag ctccagcacc ttctggacgc gctggccggt
     1501 cttggcgctg atcgacacga ccggggcgta gtcgatgaac ggcagctcgc gccggagatt
     1561 ggccgcgaac tcgtcgaagc tcttgtgcgt cttgtcctcg acgatgtccc acttgttgac
     1621 cgcgatgacg aggcccctcc cctcctccac cacgtagccg gccacgtgcg cgtcctgcgc
     1681 ggtcaggccg tcggaggcgt cgagcaccag gacggcgacg tcggcacgcg agatcgcctt
     1741 gagcgcgcgc agcgtcgagt agcgatcggc gtcgggcccg gacgcgacct tgccgcgctt
     1801 cctgatgccc gccgtgtcga tcaggacgac ctcgctccgg ccccacggga tcgtcgtgtc
     1861 gatcgcgtcc cgcgtggtcc ccgggatgtc ggacacgatc gtccgcgtct cgcccagcag
     1921 cgagttcagc aggctcgact tgccgacgtt gggccggccc acgaacgcga tcgccggcgg
     1981 ctcgtcgccc tccgcggcca tcatcgcgtc ccagcgggcg gactcggccg agtcgtcgtc
     2041 gggcaccgcc ccatcctcgc cggccgcggc ctcggtcccg tcctcgccat cggcggcccc
     2101 atcgtcgccc gtcgagaacg gttcgatccg gcccgccgcc atgtcggacg cccaggcctc
     2161 ggcctccgcc tcgcgggtct tgcgcgcggt ctcggcctcg gtctcggggg gcagcaccca
     2221 cacgacggca tcgagcaggt cccccacccc acgcccgtgg ctggccgaga tcgcatacgt
     2281 ctcctcccag ccgaaggcgt agaactcggc ggcgtccagc tcgcgcttct cgttgtcggc
     2341 cttgttggcg gccaccagca cgggcttggt cgtgcggcgc aggaggtcgg cggcgtcctg
     2401 gtcggccggc atgggcccgg tctccgcgtc gacgaggaac acgatcacgt cggcctcgct
     2461 gatcgccagc cgcgcctgga gctggacctg cgcctcgatc gggtcgccgg gatcgatctc
     2521 caggccgccg gtgtccacga cgacgaaccg ccggccgttc cactcggcgt cgccatacag
     2581 gcggtcgcgc gtcgtgcgcg cccggtcctc gacgatcgcc gcacgcgagt cgccgacgac
     2641 ccggttgaac agcgtgctct tgccgacatt cggtcgcccc acgaaggcga cgacgggcag
     2701 gccgcgggtg cgagcgggca cgggtcaggc cgcgccggtc gacgcgacgg ggtgcgcggg
     2761 cagcgaggtc gtgcggccgg cggggtgcgc ggcggcgtcg agggcgcccc gggcgaggct
     2821 cgcggcgtcg gctatcgcct cgggcgtgcc ggcaatctcg aagatgcgct ccgcgacgtc
     2881 gcgcaggtcc tcgatcgggg tcgaggtgcc gccggtgacg ccgacgactt tcgcgccctc
     2941 gaacggcgcc gggtcgacga ggtcgcgcgc gttctcgatc tggatcgcgg ccgtgccgac
     3001 gatctcgcac agccgggtca gctccttggt gttcgcggag tgccggccgc cgacgaccac
     3061 ca
//
LOCUS       DBNHXO010000511         2041 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2150366, whole genome shotgun sequence.
ACCESSION   DBNHXO010000511 DBNHXO010000000
VERSION     DBNHXO010000511.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2150366"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="AC162A_10310"
     CDS             complement(<1..1007)
                     /locus_tag="AC162A_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase family protein"
                     /protein_id="HIR5011278.1"
                     /translation="MSGRVFRRATSPEPPVAVSGHGSTIVDADGREYLDAAGGAIVVN
                     VGHGRLEIADAIAEQAGRLAYAHGSAFTTEPLERYAREVARHLPLSDPAIYPVSGGSE
                     AIETALKLARATQLARGEPDRLVVFARWGSYHGNTLGALDLSGRRPLRRPYEGWLGRF
                     RHVSAAYPYRGDDPGSQALGSTQELVDELDQAFTLADPGTVAAFVAEPIVGATLGAVE
                     PPPGYWPAIADVCRRHGALLIADEVMTGFGRTGAWFGLTHYGVQPDLLVAAKGATSGY
                     WPFGFVAASGEVYDAVIGAGPFVHGFTYSHQPVAATVALEVLRILESESLVEASAAKG
                     ER"
     gene            complement(1004..>2041)
                     /locus_tag="AC162A_10315"
     CDS             complement(1004..>2041)
                     /locus_tag="AC162A_10315"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR5011279.1"
                     /translation="RGPLLTLATIVIVVVGIGLAGSMFALLTFSALAAIVARRAQLWL
                     LDRGMASWLALVITTGGFVLILVVMGVAVVASIAAVGLALFEDSSQIEQWLGMAEDAW
                     GTVTGVPGATLPAIEPQAALGAARSVVAALAPAVTSLAFSVLIVIYVLLGAPGLRGRM
                     LRATSEGILARYDALATELVTYVKVRAALGAAAAVADTVLLLVLGVPYAVLWGLMSFL
                     FSFVPNIGFILALIPPAAFALLEHGPASAIAVVVGYVVINVAFDYVLQPRLVATTLDV
                     SPVITIVTILVWTALIGPAGALLAVPLTITLRALLMPFPGAAWFVAFLGREMAPDATD
                     AADPTEPAIPA"
BASE COUNT          334 a          754 c          732 g          221 t
ORIGIN      
        1 cgctcgccct tggcggccga tgcctcgacc agcgactccg actcgaggat ccggagcacc
       61 tcgagcgcga ccgtcgcggc gaccggctgg tgcgagtagg tgaagccgtg gacgaacggg
      121 ccggccccga tcaccgcgtc gtagacctcg cccgacgccg cgacgaaccc gaacggccag
      181 tagcccgaag tcgcgccctt ggccgccacc agcagatccg gctggacgcc gtagtgggtg
      241 agcccgaacc aggcgccggt ccgcccgaaa ccggtcatga cctcgtcggc gatgagcagg
      301 gcgccgtgcc ggcggcagac gtcggcgatc gccggccagt agccgggcgg cggttcgacg
      361 gcgcccagcg tcgccccgac gatcggctcc gcgacaaacg ccgctaccgt cccgggatcg
      421 gccagcgtga acgcctggtc cagctcgtcg accaactcct gcgtcgaccc gagcgcctgc
      481 gagccggggt cgtcgccgcg atacgggtac gcggcgctca cgtgccggaa gcgcccgagc
      541 cagccctcgt acggccggcg caggggccgc cgacccgaca ggtcgagcgc cccgagcgtg
      601 ttgccgtgat agctccccca gcgagcgaac acgaccaggc ggtccggctc accgcgcgcg
      661 agctgcgtgg cacgagccag cttgagggcg gtctcgatcg cctccgaccc ccccgagacc
      721 gggtagatcg ccgggtcgct caacggcagg tggcgggcga cctcgcgcgc gtagcgctcg
      781 agcggctcgg tggtgaacgc actgccgtgg gcgtacgcca gccggccggc ctgctccgcg
      841 atcgcgtcgg cgatctcgag ccggccgtgg ccgacgttga ccacgatggc gccccccgcg
      901 gcgtcgagat actcccggcc gtcggcgtcg acgatggtgg agccgtggcc cgacacggcg
      961 accggcggct cgggcgaggt cgcccggcgg aagacgcggc cgctcatgcc gggatcgcag
     1021 gctcggtggg gtccgcggcg tccgtggcgt ccggagccat ctcccgcccg aggaatgcca
     1081 cgaaccacgc cgcacccggg aacggcatca gcagcgctcg cagcgtgatc gtgagcggga
     1141 cagcgagcaa cgcacccgcc ggcccgatca acgcggtcca caccaggatc gtgacgatcg
     1201 tgatcacggg gctcacgtcg agcgtcgtcg ccaccagccg cggctggagg acgtagtcga
     1261 aggccacgtt gatgacgacg tagccgacga ccacggcgat ggccgacgcc gggccgtgct
     1321 cgagcagcgc gaacgcggcc ggtgggatca gggcgaggat gaagccgatg ttggggacga
     1381 agctgaacag gaacgacatc aggccccaca gcaccgcgta cggcacgccg agcacgagca
     1441 gcaggacggt gtccgcaacc gcggccgcgg cccccagcgc tgcgcgcacc ttgacgtagg
     1501 tgacgagctc ggtcgcgagg gcgtcgtacc gcgcgaggat cccctcggag gtcgcccgca
     1561 gcatccggcc gcgcagcccg ggcgcgccga gcaggacgta gatcacgatc aggaccgaga
     1621 acgcgagcga tgtgaccgcc ggcgcgagcg ccgccaccac gctgcgcgcc gctccgagcg
     1681 cggcttgcgg ctcgatcgcc ggcaacgtcg cgcccgggac accggtcacg gttccccacg
     1741 cgtcctcggc catgccaagc cattgctcga tctgtgacga gtcctcgaac agcgccaggc
     1801 cgaccgcggc gatcgaggcg acgacggcga cgcccatcac gaccaggatc aggacgaacc
     1861 cgccggtcgt gatgacgagc gccagccacg aggccatgcc ccggtcgagc agccacagct
     1921 gcgcgcgtcg ggcgacgatc gccgccaggg ccgagaacgt gagcagcgcg aacatgctgc
     1981 ccgccaggcc gatcccgacg acgacgatca cgatcgtcgc gagcgtcagc agcgggccgc
     2041 g
//
LOCUS       DBNHXO010000512         1806 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5128743, whole genome shotgun sequence.
ACCESSION   DBNHXO010000512 DBNHXO010000000
VERSION     DBNHXO010000512.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5128743"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="AC162A_10320"
     CDS             <1..1398
                     /locus_tag="AC162A_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011280.1"
                     /translation="GFAEKYGMEFRRASLDEQPDAWLIERHEREIVPLLHRRGDFAEA
                     RDFLLYDFGSDGGGVDEHVFAYSNGSGPSRSLIVYHNRYAETAGWIRDSVGFALKGAD
                     GSKAMVRRTLADGLDVADGPAEGRWLALREQRSGLEYLRSVAEIRDRGLHVALRAYET
                     RVFWELRELSDAAGVWGRLAERLGGRGVPSLEGALRELQLAPVHEALRAAISDPYDAT
                     IGRLVGVVAESTGTGGDRASVEEAVRALVDAVEPVALGIDDASQAAAIRVWALLRPLG
                     ALPTGAPVGATSRAWFEELRLAPAVADALRSMSIDEAGAWWAAERVRLLLDLPLPSTL
                     RGRGGAATLPLRLVDAWLADPVVRSFLRVNRWDDAEWFHRESWAELLAWTDRLERALT
                     PADARVRRPVERSVLARRLVEAGEASGYQIDALRAALAAPAKASGPNHGRAKPRLPKA
                     TGLPPGPKPAPKRRP"
     gene            complement(1436..>1806)
                     /locus_tag="AC162A_10325"
     CDS             complement(1436..>1806)
                     /locus_tag="AC162A_10325"
                     /inference="COORDINATES: protein motif:HMM:NF017745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HIR5011281.1"
                     /translation="NAILDVRIHTMGMTSEEAVALMVDGAFQERAEAVQKDERARLSS
                     TQLSTYFVGSLGMWDIEDAARRRAAVAAGASADAVPTPRVVGGYPATPGFDHRAHLEA
                     VISHGAPPISILRRILLADG"
BASE COUNT          215 a          650 c          706 g          235 t
ORIGIN      
        1 ggcttcgccg agaagtacgg catggagttc cggcgggcgt ccctggacga gcagccggac
       61 gcgtggctga tcgagcgcca cgagcgggag atcgtccccc tgctccaccg gcggggcgac
      121 ttcgccgagg cgcgcgactt cctgctgtac gacttcgggt ccgacggcgg cggggtcgac
      181 gagcacgtgt tcgcgtactc gaacgggtcg gggccgtcgc ggtcgctgat cgtctaccac
      241 aaccggtacg cggagacggc gggctggatc cgcgactcgg tcgggttcgc gctcaagggg
      301 gccgacgggt cgaaggcgat ggtgcgccgg acgctggcgg acgggctcga cgtggccgac
      361 gggccggccg agggccgctg gctcgcgttg cgcgagcagc ggtcgggcct cgagtacctg
      421 cgctcggtcg ccgagatccg ggaccgcggc ctccacgtcg cgctgcgggc ctacgagacg
      481 cgcgtgttct gggagctgcg cgagctgtcc gacgccgcgg gcgtgtgggg ccggctcgcc
      541 gagcggctgg gcggtcgcgg cgtgccgtcg ctcgagggcg ccctccgcga gctgcagctg
      601 gcgcctgttc atgaggccct gcgcgccgcg atcagcgacc cctacgacgc gacgatcgga
      661 cgcctggtcg gcgtcgtggc cgagtccacc ggcacgggcg gggaccgagc gtcggtcgag
      721 gaggcggtgc gagcgctcgt cgacgcggtg gagccggtcg cgctcgggat cgacgacgcg
      781 tcccaggccg cggcgatccg cgtctgggcg ctgctccggc cgctcggagc gctccccacg
      841 ggtgcgccgg tgggggcgac gagccgggcg tggttcgagg agctgcggct cgcgcccgcg
      901 gtcgcggacg cgctgcgatc gatgagcatc gacgaggcgg gggcgtggtg ggcggccgag
      961 cgggttcgcc tcctgctgga cctgccgctg ccgtcgacac tcaggggacg cggtggtgca
     1021 gccacgctcc ccctccgcct ggtggacgcg tggctcgccg acccggtcgt ccgctcgttc
     1081 ctgcgggtca accgctggga cgacgccgag tggttccacc gcgagtcgtg ggcggagctg
     1141 ctcgcctgga cggaccggct cgagcgcgcc ctgacacctg cggacgcccg cgttcgacgt
     1201 cccgtcgagc gctcggtcct cgcccggcgg ctggtcgagg ccggcgaagc gtccggctac
     1261 cagatcgatg cgctcagggc cgcgctcgcc gcgccggcga aggcctcagg cccgaatcac
     1321 gggagagcga agccgcgcct gccgaaggcc accggcctgc cgccgggccc gaaaccagcc
     1381 cccaagcgcc gtccctgacc cgcgccgacc ggaggcccgg gaacggcgcc cgggcctagc
     1441 cgtcggcgag caggatccgg cgcaggatcg agatcggcgg ggcgccgtgg gagatcacgg
     1501 cctcgaggtg ggcacggtgg tcgaagcccg gggtcgccgg gtagccgccg accacccgcg
     1561 gcgtcggcac cgcatccgcc gaggcgccag cggccaccgc cgcacgccgt cgcgcggcgt
     1621 cctcgatgtc ccacatgccg agcgacccga cgaagtacgt cgacagctga gtggagctga
     1681 gccgcgcgcg ctcgtccttc tggacggcct cggcccgctc ctggaacgcg ccgtcgacca
     1741 tcagcgcgac cgcctcctcg ctcgtcatcc ccatcgtgtg gatccgcacg tcgaggatcg
     1801 cgttgg
//
LOCUS       DBNHXO010000513         7555 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1161395, whole genome shotgun sequence.
ACCESSION   DBNHXO010000513 DBNHXO010000000
VERSION     DBNHXO010000513.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7555)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7555
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1161395"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="AC162A_10330"
     CDS             <1..870
                     /locus_tag="AC162A_10330"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HIR5011282.1"
                     /translation="VGRIVYTVLANERGGIEMDPTITRLGASTFLVLAPTLSQRRTMG
                     LLRNGLPDDAVVTDVNAGFATLHVAGPRSRELLARLTDEDLSTEAWPFLRAREIEVGR
                     VTGWAFRVSYTGELGWELMVSTELVADLYEHLVAAGTDLGLRHAGSFAFEAARLERGF
                     RAWGHDVGPLDDPFAAGLGFTVSRRKAAEYVGREALERLRANLELERRLISVHAPDAV
                     LWHGESMLRDGERVGFVTSASIAPTLGGSVGLAWIRGPVEGDWQVEVRAEAVPCRVAV
                     DPFYDPGGARLRG"
     gene            886..2445
                     /locus_tag="AC162A_10335"
     CDS             886..2445
                     /locus_tag="AC162A_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HIR5011283.1"
                     /translation="MDVLTALIIELVVIAVLFEVARRLGLPYPALFVLGGLALGVVPG
                     IPHITLEPDLVLLVFLPPLLFAAAFETPLRDLRTNLWPIARLSLGLVVLTTVVVAAVA
                     QALVPGMGWAAAFALGAIVAPTDALAATSVFRRLGVPRVILSLIEGEALFNDASALIL
                     YRAAVAAVLSGTFVFSDAIGNFVVAGIGGIVLGFVVGRVMAEVVRWLDDPPVEVVVSI
                     LIPFLAFLPAEEFGLSGVLAVVTAGLVIGRRLGTILSPSSRLLWLTSWKMVTFVLNGL
                     VFVLVGLALPDILAGPAMISPAQFIGLAILVCGAVIGTRFAWVFLSSLLPRSPRRLIA
                     DRDPALAWRLTFLVGWSGLRGAVSLAAALALPLDVPARNLILLLTFAVILATLVGQGL
                     TLPAVVRWVHWDGVEAEADEVLLARTTMYQVGRGGIRLARGRWPDHQPLLDRLDAGLQ
                     YREEHLATEDMAESDEGSEDHLAHQEIQLSIINAQRGAIIALRDAGEINDATLRTLER
                     ELDLEELRMEA"
     gene            complement(2451..3350)
                     /locus_tag="AC162A_10340"
     CDS             complement(2451..3350)
                     /locus_tag="AC162A_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013805618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR5011284.1"
                     /translation="MGNAGWTVAGLPDLTGRTVVVTGASSGIGAATARALAGAGATVV
                     LAVRDVVKGERVAATIAGTTEVRELDLENLASVRGFAAAWSGDVDILVNNAGIMMVPE
                     GRTADGFERQIGTNHLGPFALTNLLLPRITDRVVTVSSNAHERGRIDLDDLNWEHRPY
                     QSSQAYSDSKLANLLFTFELQRRLTAAGSSVRALAVHPGMVRTNLFGHASGVTATLIG
                     ALGPLVMQDPANGARPTLFAATQDVPGGSFVEPDGLAHLRGSPTIARASRSAYDAEVA
                     GRLWDLSARLTGVASPVAVAAGA"
     gene            complement(3367..4371)
                     /locus_tag="AC162A_10345"
     CDS             complement(3367..4371)
                     /locus_tag="AC162A_10345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020127328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR5011285.1"
                     /translation="MKAITQDTYGAAAVLKTRDIDKPQIGEGQVLVRVRAAGVNIADW
                     AIMTGLPYIARPVYGMRRPKIAIRGTDVAGVVEAVGPGVTRLKPGDEVFGWCTGSFAD
                     YAAASEDGLALKPANLTFEQAASVPMAGLVALLALRDHGKIKAGQRVLVNGAAGGIGT
                     FAVQIAKSMGAEVTGVCSTRNVEMVRSIGADHVIDYTEQDFTKSGEQYDFILDNVANH
                     SLTELRRVLTPTGMLVPNGGGFQHRWMASGGRLVRAAVMFRFGDQKLGNFLLAQKLDD
                     LLALKALIEAGKVTPVMDRAFSLSETREAIDHVATGHARGKVAITVSRSQPVALPAVA
                     "
     gene            4528..5187
                     /locus_tag="AC162A_10350"
     CDS             4528..5187
                     /locus_tag="AC162A_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5011286.1"
                     /translation="MARAEHRNRLSRERVLRAAVDQADAGGLDELTMRKLAEVLEVAP
                     MALYRHVANKDDLVDAMIDAVFAEIDLPSADGDWRTAMHGRAISVRDALARHRWAIGL
                     MESGTNPGPANLRHHDAVIGSLRAGGFDIANAAHAYSLLDSYIYGFALTKMNLPFEGS
                     DEIADVAQEMLAPFPEGEYPHLMEILTEHVMLPGYDYGDEFEYGLDLILDGLERVRAT
                     A"
     gene            complement(5235..5654)
                     /locus_tag="AC162A_10355"
     CDS             complement(5235..5654)
                     /locus_tag="AC162A_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015656404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HIR5011287.1"
                     /translation="MLKTRLLHPEILQALAEAGHGARVLIADGNYPLATQSNPAGRRV
                     YLNLEPGRLSVVEVLDVLVEAIPIEAAHVMGPDDGSEPTIFAEFGRALPDTELQRLGR
                     FEFYAAASAPDTALSIATGEQRLYANILLTIGVVLPG"
     gene            5864..7207
                     /locus_tag="AC162A_10360"
     CDS             5864..7207
                     /locus_tag="AC162A_10360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5011288.1"
                     /translation="MDVGDLGVRLAGDLVLPGDERWDTARQAWNLAVDQRPVAIVYPE
                     SAEDVAQTVRFAAERGLRIAFNAGGHNAGPIDWSRDTLLLKTERMRGVEIDAAARRAR
                     IEAGVQGKPLALAAAEHGLAYLSGTSPDVGVVGYSLGGGLSWMIRTFGLACNSIRAAE
                     VVTADGRLVRTDRDTEPDLFWALRGGGGNVAAVTALELELFPVAEIYAGALFWPIERA
                     TEILNAWRTWIETVPEACESLGRMLQFPDLPFLPDHLRGRSFVLVEAAIIGSEADAAA
                     LIRPLRDLGPEFDTVALMPASDLSLVNMDPDFPLPYAGDGILLNDLDAAAIDEIVTAF
                     VGSPLLHVEVRHLGGAAGRRSPDHGVLDAIDQPFVCFTFGIAPDAEAVEVVEHHARQL
                     LGALGPWDSGRRYLNFAESPMDPRSIFPVETYERFRQVKARYDPAGMFRANHAID"
     gene            complement(7223..>7555)
                     /locus_tag="AC162A_10365"
     CDS             complement(7223..>7555)
                     /locus_tag="AC162A_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011289.1"
                     /translation="AWGLAGTWTVARHAAVLNEPGGRIAFQFHARDVNLVMGPASRGA
                     AIRYRVYLDGQLAWGALGSDVSPDGSGIVDDQGTYQLIRTPGQVAERRFEIEFLDSGV
                     EAYCFTFG"
BASE COUNT         1008 a         2653 c         2693 g         1201 t
ORIGIN      
        1 gtcgggcgca tcgtctatac ggtcctcgca aacgagcgcg gcgggatcga gatggacccg
       61 acgatcacgc gcctcggcgc ctcgacgttc ctcgtgctgg ctccgacgtt gagccagagg
      121 cggacgatgg gcctgctgcg caacggcctg ccggacgacg ccgtcgtcac cgacgtcaac
      181 gctggtttcg cgacgctcca cgtcgcgggg ccgcggtcgc gcgagctcct cgcgcgcctg
      241 accgacgagg acctctcgac cgaggcgtgg ccgttcctcc gtgcccgcga gatcgaggtc
      301 ggccgggtga ccggctgggc cttccgcgtc tcctacacgg gtgagctggg ctgggagctc
      361 atggtctcga cggagctcgt ggccgacctg tacgagcacc tcgtggcggc cggcacggac
      421 ctcgggctgc ggcacgccgg gtcgttcgcg ttcgaggccg cgcggctcga gcgcgggttc
      481 cgcgcctggg gccacgacgt cggcccgctc gacgacccgt tcgcggccgg gctcgggttc
      541 acggtgtcgc gccggaaggc ggccgagtac gtgggccgcg aggcgctcga acgcctgcgc
      601 gcgaacttgg agctcgagcg ccggctcatc tcagttcacg ccccggacgc cgtcctgtgg
      661 cacggcgagt cgatgctccg cgacggcgag cgggtcgggt tcgtcaccag cgcttcgatc
      721 gcgccgaccc tcggcgggtc cgtcggactc gcctggatcc gcggaccggt cgagggcgat
      781 tggcaggtcg aggtccgagc ggaggccgtc ccgtgccgcg tcgccgtcga tccgttctac
      841 gacccgggag gcgcgcggct ccgcggctag gcatcatcgt cctgcatgga cgtcctcacc
      901 gccctcatca tcgagctggt cgtcatcgcg gtcctgttcg aggttgcgcg ccgcctgggc
      961 ctgccctacc ccgccctctt cgtgctgggc ggcctggcgc tcggggtcgt cccggggatt
     1021 ccgcacatca ccctcgagcc ggacctcgtg ctgctcgtct tcctgccgcc gctcctgttc
     1081 gcggcggcgt tcgagacgcc cctgcgtgac ctgcgcacca acctgtggcc gatcgcccgg
     1141 ctgtcgctcg ggctcgtcgt cctcaccaca gtcgtcgtcg cggccgtcgc gcaggccctg
     1201 gtccccggga tgggctgggc cgccgcgttc gcgctgggtg cgatcgtcgc cccgaccgac
     1261 gcgctggccg cgacgagcgt gttccgacgc ctcggcgtgc cgcgcgtgat cctgtccctg
     1321 atcgagggcg aggcgctgtt caacgatgcg tccgccctga tcctgtaccg cgccgcggtg
     1381 gccgcggtgc tgtccgggac gttcgtgttc agcgacgcca tcgggaactt cgtggttgcc
     1441 gggatcggcg gcatcgtcct cgggttcgtc gtcggccggg tgatggctga ggtcgtccga
     1501 tggctcgacg acccgcctgt cgaggtcgtc gtctcgatcc tcatcccgtt cctcgccttc
     1561 ctgcccgccg aggagttcgg gctgtcgggc gtgctggcgg tggtgaccgc cgggctcgtg
     1621 atcgggcgac gactggggac gatcctctcg cccagcagcc ggctgctgtg gttgacgagc
     1681 tggaagatgg tcacgttcgt cctcaacggc ctggtcttcg tcctcgtcgg cctcgcgctg
     1741 cccgacatcc tcgccggccc ggcgatgatc tcgccggccc agttcatcgg cctggcgatc
     1801 ctcgtgtgcg gcgcggtgat cgggacgcgg ttcgcctggg tcttcctctc gagcctgctg
     1861 ccgaggtcgc cgaggcgcct gatcgccgat cgcgacccgg cacttgcctg gcggctcacg
     1921 ttcctggttg gctggtccgg cctgcggggc gccgtgtcgc tggccgccgc cctcgcgctc
     1981 ccgctggacg tcccggcgcg caacctgatc ctgctgctga cgttcgcggt gatcctcgcg
     2041 acgctggtcg gccaggggct gacgctgccg gccgtcgtgc gctgggtcca ctgggacggc
     2101 gtggaggccg aggccgacga ggtgctcctg gctcggacga cgatgtacca ggtcgggcgg
     2161 ggcggcatcc ggctggcgcg cgggcggtgg cccgatcatc agccactcct ggaccgcctc
     2221 gacgccgggc tccagtaccg cgaggagcac ctcgccacgg aggacatggc cgagtccgac
     2281 gaggggagcg aggaccacct cgcccaccag gagatccagc tcagcatcat caacgcgcag
     2341 cgcggcgcga tcatcgccct ccgcgatgcg ggcgagatca acgacgcgac gctgcgcacg
     2401 ctggagcggg agctcgacct cgaagagctg cggatggagg cctgaggccg ctacgccccg
     2461 gcggcgacgg cgaccggcga ggcgaccccg gtgaggcggg cggagagatc ccacagccgt
     2521 ccggcgacct cggcgtcgta ggcgctgcgc gacgccctcg cgatggttgg gctgccgcgg
     2581 aggtgagcga gtccgtcggg ctccacgaag gagccgcccg ggacgtcctg cgtcgcggcg
     2641 aacagcgtcg ggcgtgcgcc attggcaggg tcctgcatga caagcggacc gagcgcgccg
     2701 atcagcgtcg ccgtgacccc ggatgcatga ccgaacaggt tcgtgcgcac catgcccggg
     2761 tgcaccgcga gggcccggac gctcgatccg gccgcggtga gccgccgctg cagctcgaac
     2821 gtgaacagca ggttggcgag cttgctgtcg ctgtacgcct ggctcgactg gtacgggcgg
     2881 tgttcccagt tgaggtcgtc gaggtcgatc cggccgcgtt cgtgcgcatt ggatgaaacc
     2941 gtcaccaccc ggtcggtgat ccgcggcagc agcaggttgg tcagcgcgaa cggcccgagg
     3001 tggttcgtcc cgatctgccg ttcgaagccg tcggccgtcc gaccctccgg gaccatcatg
     3061 atcccggcgt tgttgacgag gatgtcgacg tcgccgctcc acgcggcggc gaagccccgc
     3121 accgatgcca ggttctcgag gtcgagctcc cgcacctcgg tcgtgccggc gatggtcgcg
     3181 gcgacccgct cgcccttgac cacgtcacgg acggcgagga cgaccgtcgc ccccgcgccg
     3241 gcgagcgcgc gcgcagtggc ggccccgatc ccgctcgagg ccccggtcac cacgaccgtc
     3301 cgcccagtca ggtcgggaag tccggccacc gtccagcccg cgttccccat cggtcttgcc
     3361 tcgcggtcag gcgactgccg gcagcgccac gggctgcgag cgcgacacgg tgatcgcgac
     3421 cttgccccga gcgtggcccg tcgcgacgtg gtcgatcgcc tcgcgcgtct cgctcagcga
     3481 gaaggcgcgg tccatcaccg gggtgacctt accggcctcg atcagggcct tgagggcgag
     3541 cagatcgtcg agcttctgcg cgagcaggaa gttcccgagc ttctggtcgc cgaagcggaa
     3601 catgacggca gccctgacca ggcgcccgcc gctggccatc cagcgatgct ggaagccgcc
     3661 gccgttgggc acgagcatgc cggtcggcgt gagcacgcgc cggagctcgg tcagcgagtg
     3721 attcgccacg ttgtcgagga tgaagtcgta ctgctcgccg ctcttcgtga agtcctgctc
     3781 ggtgtagtcg atgacgtggt cggcaccgat cgatcggacc atctccacgt tgcgcgtgct
     3841 gcacacgccg gtcacctcgg cacccatcga cttcgcgatc tggaccgcga atgtgccgat
     3901 gccgccggcg gcgccgttca cgaggacccg ctggcccgcc ttgatcttgc cgtggtcgcg
     3961 cagcgccagg agcgcgacga ggccggccat cgggacggag gctgcctgct cgaaggtgag
     4021 gttggcgggc ttgagcgcca gaccatcctc cgatgcggcg gcgtagtcgg cgaacgagcc
     4081 ggtgcaccag ccgaacacct cgtcgcccgg cttgagccgg gtcacgccag ggccaacggc
     4141 ctcgaccacg cccgccacgt ccgtccctcg gatggcgatc ttcggcctgc gcatgccgta
     4201 gacagggcgg gcgatgtagg gcaggccggt catgatggcc cagtcggcga tgttgacgcc
     4261 ggccgcacgc acgcggacga ggacctggcc ctcgccgatc tgcggcttgt cgatgtcgcg
     4321 agtcttgagg acggcggccg cgccgtaggt gtcctgggtg atggccttca tcgggatggg
     4381 tctccgcttc gttccgcacg ttcgatgcgt acgttgtacg cttgtggtgt acgttgtacg
     4441 ctaacgcgta cgatgtacgc tgtcaaggcc ggggttgacc gtcctgcaga agacacacga
     4501 ggggtgaagt cagtggtcac tcgcgcgatg gctcgggccg agcatcgcaa tcgcctgagc
     4561 cgggaacgcg tccttcgcgc cgcggtcgac caggccgatg ccggcgggct cgatgagctc
     4621 acgatgcgca agctcgccga agtcctcgag gtcgcgccga tggcgctcta ccgccacgtc
     4681 gccaacaagg acgacctcgt cgacgcgatg atcgacgccg tctttgccga gatcgacctg
     4741 ccgtcggccg acggcgactg gcgaacggcg atgcacgggc gcgcgatctc ggtccgggac
     4801 gccctggcgc ggcaccgttg ggcgatcggc ctgatggagt cagggacgaa ccccggtccc
     4861 gcgaacctgc gccaccacga cgcggtgatc ggcagcctgc gggcgggcgg cttcgacatc
     4921 gcgaatgccg cgcacgccta ctcgctcctc gacagctaca tctacggctt cgcgctgacg
     4981 aagatgaacc tgccattcga gggttccgac gagatcgccg atgtcgccca ggagatgctc
     5041 gcgccgttcc cggagggcga gtacccccac ctcatggaga tcctgaccga acacgtgatg
     5101 ctgcccggct acgactacgg cgacgagttc gagtacggcc tcgacctcat cctcgacggc
     5161 ctggagcgcg tccgggcgac cgcgtgaacg aagtcgacga cgacgcgccg accgtcgaca
     5221 ggttgcggcg ggcgtcagcc gggcaggacc acgccgatcg ttagcaggat gttcgcgtaa
     5281 agccgttgct ccccggtggc aatggataac gccgtgtccg gcgcactggc cgccgcgtag
     5341 aactcgaaac ggccgaggcg ctgcaattcc gtgtccggca gggcgcgtcc gaactccgcg
     5401 aagatcgtcg gctcggagcc gtcgtccgga cccatcacgt gtgccgcctc gatcgggatg
     5461 gcctcgacga gcacgtcaag cacctcgacg acgctgagcc gcccgggctc gaggttgagg
     5521 tagacccggc gcccggccgg attcgactgc gtcgccagtg ggtagttgcc atcagcgatc
     5581 agcacgcggg cgccatggcc ggcctccgcg agcgcctgga ggatttccgg gtggagcagg
     5641 cgagtcttga gcatcaggct ccttggttgg tccgaacatc agctcccgag cggggccgcc
     5701 ggccacggtg cgagatcacc tccggcatgt caaggtggcc gtggccgtga gttccggggt
     5761 gcgggcgtgc cccaaacggg aacggggcgg tatccgaaga tgttcgtcgt cgagtcgttt
     5821 cgcggctggc gacccgtcgc cggcgcttca gcggaggtgg cacatggatg tgggcgatct
     5881 cggggtccga cttgccggcg acctcgtcct gcccggcgac gagcgctggg acaccgcgcg
     5941 gcaggcgtgg aacctggccg tcgaccagcg ccccgtcgcg atcgtctacc ccgagtcggc
     6001 ggaggacgtc gcgcagaccg tccgctttgc cgccgagcgc ggcctgcgca ttgcgttcaa
     6061 cgccggcggt cacaacgcgg ggccgatcga ctggagccgg gacacgctgc tgctcaagac
     6121 cgagcggatg cgcggggtcg agatcgacgc cgcggcgcgg cgcgcccgga tcgaggcggg
     6181 ggtccagggg aagccgctgg cgctcgcggc cgccgagcac ggcctcgcgt acctctccgg
     6241 cacgtcgccc gatgtcggcg tggtcgggta ttcgctcggg ggcggcctca gttggatgat
     6301 ccggaccttc gggctcgcct gcaacagcat ccgggccgcc gaggtcgtga ccgcggatgg
     6361 ccggctggtt cgaacagacc gcgacacgga gcctgacctg ttctgggcgc tccgcggggg
     6421 cggcggcaac gtcgccgcgg tgaccgcgct cgagctcgag ctgttcccgg tggccgagat
     6481 ctacgccggg gcgctgttct ggccgatcga gcgggcgacg gagatcctca acgcctggcg
     6541 cacctggatc gagacggtgc ccgaagcctg cgagtccctc ggtcggatgc tccagttccc
     6601 ggacctgccc ttcctccccg accacctgcg cggccggtcc ttcgtcctcg tcgaggcggc
     6661 gatcatcggc agcgaggctg atgcggcggc gctcattcgg ccgcttcgcg acctcggccc
     6721 ggagttcgac acggtcgcgc tgatgccggc gagcgacctc agcctcgtca acatggatcc
     6781 ggacttcccg ctgccctacg cgggcgacgg catcctgctc aacgacctgg atgcggccgc
     6841 gatcgacgag atcgtgacgg cgttcgtcgg ttcacccttg ctgcacgtcg aggttcgcca
     6901 cctgggcggc gcggcgggcc ggcgatcgcc ggatcatggg gtgctcgacg cgatcgatca
     6961 gcccttcgtc tgcttcacct tcgggatcgc gcctgacgcg gaggcggtcg aggtcgtgga
     7021 gcaccacgcc cggcagctgc tcggggcgct cgggccgtgg gacagcgggc ggcggtacct
     7081 caacttcgcc gagtcgccga tggacccgcg ctcgatcttc ccggtcgaga cctacgagcg
     7141 cttccggcag gtgaaggcgc gctacgaccc ggccggcatg tttcgggcca accacgcgat
     7201 cgactgacgg ccgcccgccg gctcagccga aggtgaagca gtaggcctcg acgcccgagt
     7261 cgaggaactc gatctcgaac cggcgctcgg cgacctgccc cggcgtacgg atcagctggt
     7321 acgtgccctg gtcgtccacg atcccgctgc cgtccgggct cacgtcgctc cccagggcgc
     7381 cccacgcgag ctggccgtcg agatagaccc ggtaccggat cgcggcgcca cgggatgccg
     7441 gtcccatcac gaggttgacg tcgcgcgcgt ggaactggaa cgcgatccga ccgccgggct
     7501 cgttcaggac cgccgcatgc cgggccacgg tccaggtccc ggcgaggccc cacgc
//
LOCUS       DBNHXO010000514         3440 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1952985, whole genome shotgun sequence.
ACCESSION   DBNHXO010000514 DBNHXO010000000
VERSION     DBNHXO010000514.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1952985"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="AC162A_10370"
     CDS             <1..281
                     /locus_tag="AC162A_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR5011290.1"
                     /translation="EREGLHEIFRAAGAEWRESGCSMCIAMNGDQLSPGQYAISTSNR
                     NFEGRQGKGGRTFLASPLTAAASALSGVVTDPRTLPGIEAPATVGGGY"
     gene            283..912
                     /gene="leuD"
                     /locus_tag="AC162A_10375"
     CDS             283..912
                     /gene="leuD"
                     /locus_tag="AC162A_10375"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR5011291.1"
                     /translation="MPDPYRGFSSAVVPLAAENVDTDQIVPARYLKVTDKEGLADALF
                     RDWRFEADGSRKDPLFILDRPGIAGRQIMLVGDNFGTGSSREHAPWALRAWGVRAILS
                     TSYADIFRSNSLKNGVLPIVVDPATHRRLFDLIERDPDAELRVDLAEQGVLLPDGSTI
                     EFEIDPFAKAMLLAGTDEIGYVMGHEDAIAAWEAANPPRVDTRLGTAAG"
     gene            1137..1751
                     /locus_tag="AC162A_10380"
     CDS             1137..1751
                     /locus_tag="AC162A_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011292.1"
                     /translation="MTTFRTRSALAALALVAVAILIAGCAAGSPAALPAPTPAASPAA
                     SAPAPTPVPGSTDSGAPGPDDPVSGGGSDPNTGTGSGGDPGVVDPGEPEPTIVAPGAG
                     LTGVHPVGAAKLDTAINGRDLAVRVSWWSGVAPCNVLAGVDVARDGTTFNLTVREGSA
                     AAPDTACIEIAQYKATIVDLGELEPGSYTITAFGDAAPVTVTID"
     gene            1810..2985
                     /locus_tag="AC162A_10385"
     CDS             1810..2985
                     /locus_tag="AC162A_10385"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HIR5011293.1"
                     /translation="MNLFDLFAVIVLVFAILAGIRTGALPQVGGVAGAIGGLLLVLAL
                     APSLLDLTADLDPIPRALVVLGVVLAAVVIGEAIGSGLGRVLAGQLGRGVLSSMDRAA
                     GGLLGAIQALLIVWLVGGLLAAGPFPTLGRAATQSMVVRGVDQVLPPPTEVVGEIAGA
                     LDASGMTDVFVGLDPLPLLPVDTPTDPQATRIAQAAIGGTARITTRACNSQVNGTGVI
                     IAPGYVVTNAHVVAGASAVRVALDTGVVDAVPVLFDPSLDVAVLYAPRLAGRSLRFAA
                     AVPERGSEGAAIGYAGGGGLVVLPAAVAGSYEATGRDIYDRTQVRREIIELRAAIEPG
                     DSGGPLILENGTIGGLVFAESRADPDVGYALSPIAVSTRVMPAIGRTGAIDVGPCID"
     gene            3059..>3440
                     /locus_tag="AC162A_10390"
     CDS             3059..>3440
                     /locus_tag="AC162A_10390"
                     /inference="COORDINATES: protein motif:HMM:NF017745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HIR5011294.1"
                     /translation="MDLTAAPPDRATPDETAALDALATAYWDAHLEADPLFATAIGDQ
                     RFDDHLPDPTPEGTAATRARYRHLLDRVDDLDAGALAGEARITLSMLRESIASDIAEL
                     DTGLLDWNVDPIEGVPAMFLLVPDY"
BASE COUNT          437 a         1309 c         1230 g          464 t
ORIGIN      
        1 ccgagcgcga gggcctccac gagatcttcc gcgccgcggg cgccgagtgg cgcgagtccg
       61 gctgctcgat gtgcatcgcg atgaacggcg accagctctc gcccggccag tacgcgatca
      121 gcacgtcgaa ccgcaacttc gagggccggc agggcaaggg cggccggacg ttcctggcct
      181 cgcccctgac cgcggccgcg tcggcgttga gcggcgtcgt caccgacccg cggacgctgc
      241 cggggatcga ggcgccggcc accgtcggcg gcgggtactg acatgccgga tccctaccgc
      301 ggcttcagct cggccgtcgt cccgctggcg gccgagaacg tcgacaccga ccagatcgtc
      361 ccggcccggt atctcaaggt gaccgacaag gagggcctcg cggacgcgct gttccgcgac
      421 tggcgcttcg aggcggacgg gtcgcgcaag gacccgctgt tcatcctcga ccggccgggg
      481 atcgccggcc gccagatcat gctcgtcggc gacaacttcg gcaccggcag ctcgcgcgag
      541 cacgcgccgt gggcgctgcg cgcctggggt gtgcgcgcga tcctgtcgac ctcctacgcc
      601 gatatcttcc gcagcaactc gctcaagaac ggcgtcctcc cgatcgtggt cgacccggcg
      661 acccaccggc ggctgttcga cctgatcgag cgcgaccccg acgccgagct gcgggtcgac
      721 ctcgcggaac agggcgtgct gctgcccgac ggctcgacga tcgagttcga gatcgacccg
      781 ttcgccaagg cgatgctcct cgccggcacc gacgagatcg ggtatgtcat gggacacgag
      841 gatgcgatcg ccgcctggga ggccgcgaac ccgccgcggg tcgacacgcg tctcggcacg
      901 gcggccggct aaccgtccgt cgcgggtggc tcgtgccact cccgcgcgca caacggcaca
      961 caccttcgag atccggggag ccggacgcgc aatcggagcc gatgtcggcg cgccgctcat
     1021 caaatgtggc tcggcagtgg ccaaatacag gagccaggcg cccttgcgcg agtcggggtg
     1081 gcgcggcagg tcggaacccg cgggaaccag gcgccgcgcg gcgccgtctg cgggccatga
     1141 ccaccttccg aacccgttcc gccctcgccg cgcttgcgct cgtcgcggtc gccatcctca
     1201 tcgccggctg cgcggccggc tcccctgcag ccttgcctgc gccgacaccc gccgcgagcc
     1261 ccgcagcctc ggcgccggct cctacgcccg tccccgggtc cacggactcc ggcgcccccg
     1321 gccccgacga ccccgtctcg ggcgggggct ccgacccgaa taccggtacg ggcagcggtg
     1381 gcgaccccgg cgtcgtcgac ccgggcgagc cggagcccac gatcgtcgcg ccgggcgccg
     1441 gcctcaccgg tgtgcatccc gtcggcgcgg cgaagctgga cacggcgatc aacggccgcg
     1501 acctcgcggt gcgggtgtcc tggtggagcg gggtcgcgcc gtgcaacgtg ctcgcgggcg
     1561 tcgacgtcgc ccgcgacggc accacgttca acctgaccgt ccgcgagggc agcgccgcgg
     1621 cgccggacac cgcgtgcatc gagatcgcgc agtacaaggc gacgatcgtg gacctcggcg
     1681 agctcgagcc gggcagctac acgatcaccg cgttcggcga cgccgcaccg gtgacggtga
     1741 cgatcgactg acccggctgg gccggatggc ccggcggcag gcatcgggca cctccccgat
     1801 actcgggccg tgaacctgtt cgacctgttc gcggtcatcg tcctcgtctt tgcgatcctc
     1861 gccggaatcc gcaccggcgc tctgccccag gtcggcggcg tcgcgggcgc catcggcggg
     1921 ctgctcctgg tcctcgccct cgcaccgtcc ctcctggacc tcaccgccga cctcgacccg
     1981 atcccgcgcg cgctcgtggt gctcggtgtc gtgctcgcgg cggtggtgat cggcgaggcg
     2041 atcgggtccg ggctcgggcg ggtcctggca ggccagctgg gtcgcggcgt cctgtcgagc
     2101 atggaccgcg ccgccggcgg gctgctgggc gccatccagg ccctcctgat cgtgtggctc
     2161 gtgggcggac tgctcgccgc ggggccgttc ccgaccctcg gccgggcggc gacccagtcg
     2221 atggtggtgc ggggcgtcga ccaggtcctg ccgccgccga ccgaggtcgt gggcgagatc
     2281 gccggtgccc tcgacgcgtc cgggatgacc gacgtgttcg tcggccttga ccccctgccc
     2341 ctgctgccgg tcgacacccc gaccgacccg caggccacgc ggatcgcgca ggcggcgatc
     2401 ggcggcacgg cgcgcatcac gacccgcgcg tgcaacagcc aggtcaacgg cacgggcgtg
     2461 atcatcgccc cgggctatgt ggtcaccaac gcccacgtcg tcgccggcgc gagcgccgtc
     2521 cgcgtcgcgc tcgacaccgg cgtggtcgac gccgtgccgg tgctgttcga tcccagcctc
     2581 gacgtggcgg tcctgtacgc gccgcgcctc gccggccgat ccctccggtt cgcggcggcg
     2641 gtcccggagc gtgggtcgga gggcgcggcg atcgggtatg ccggtggcgg ggggctggtc
     2701 gtcctgccgg ccgcggtcgc gggctcctac gaggccaccg gacgcgacat ctacgaccgc
     2761 acccaggtcc gccgcgagat catcgagctg cgcgccgcga tcgagcccgg cgactcgggc
     2821 gggccgctga tcctcgagaa cggcacgatc ggcgggctcg tgttcgccga gtcgcgtgcc
     2881 gaccccgacg tggggtacgc gctgagcccg atcgccgtct cgacccgcgt gatgccggcg
     2941 atcggccgga cgggcgcgat cgacgtgggg ccgtgcatcg actgacgctg gctacggtgt
     3001 cgggcggccg acacgacggc gcggcccggg cgagggtcgc ggtcgatacg atctgccgat
     3061 ggacctgacg gcggcaccgc ccgaccgcgc aaccccggac gagaccgccg ccctcgacgc
     3121 gctcgcgacc gcgtactggg acgcccacct cgaggccgac ccgctgttcg ccaccgcgat
     3181 cggcgatcaa cgcttcgacg accacctgcc cgacccgacg ccggagggaa cggcggccac
     3241 gcgcgcgcgc taccgccacc tcctcgaccg ggtggacgac ctggatgccg gcgcgctggc
     3301 cggcgaagcg cgcatcacgc tgtcgatgct gcgcgagtcg atcgcgtcgg acatcgccga
     3361 gctcgacacg ggcctgctcg actggaacgt cgatcccatc gagggcgtgc ccgcgatgtt
     3421 cctgctcgtc cccgactacc
//
LOCUS       DBNHXO010000515         1783 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1953770, whole genome shotgun sequence.
ACCESSION   DBNHXO010000515 DBNHXO010000000
VERSION     DBNHXO010000515.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1953770"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /gene="ftcD"
                     /locus_tag="AC162A_10395"
     CDS             complement(<1..937)
                     /gene="ftcD"
                     /locus_tag="AC162A_10395"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HIR5011295.1"
                     /translation="MLVESVPNVSEGRRLDVVDRLADALVGTPGVFLLDRTSDASHNR
                     SVFTIAGEHEPVAAGLEEMVGRALDEIDMETHTGEHPRIGAVDVVPFVPLADTPMDAC
                     VEMARAFGRRIAARWDLPVYLYAEAAARADRVRLADVRRGQYEGLKVEITHNGRQPDY
                     GPERMHPRAGAVAVGARPFLIAWNINLASDDVELAKRIARRVRESGGGLPRVQANGFR
                     VEEPERGHPARAQVSMNLLNARTTPMWRAWEVVGELAAEDGVELAESELIGLAPLAAF
                     LDVADHAGAPSDADTATRLAAAAEAIQLRDFDPLMA"
     gene            1038..>1783
                     /locus_tag="AC162A_10400"
     CDS             1038..>1783
                     /locus_tag="AC162A_10400"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5011296.1"
                     /translation="MTEPRRSLLHHPDFLKLWTAETISVFGTAVSQLALPLIAATILQ
                     VSAFEFGLLTTIAFLPFILLSLPAGVWVDRLRRRPILILGDLGRAIAIASIPIAFAFD
                     ALTIWQLYVVGFVNGCLTVFFDVAYQSYLPSIVERDQRVEGNSKLEITRSAAQILGPG
                     LAGVLIGAFRAPFAMVIDAVSYLWSAVFVFLIRRPEPPVVPHDEAAHGPKPSMRKEIA
                     IGLRYVTGHRWLRSIAATTGTANFFGNVMGA"
BASE COUNT          232 a          657 c          618 g          276 t
ORIGIN      
        1 gggccatcag cgggtcgaag tcgcggagct ggatcgcctc cgcggccgct gcgagccggg
       61 tcgcggtgtc ggcgtcgctc ggcgcacccg cgtggtcggc gacgtcaagg aacgcggcca
      121 gcggcgcgag cccgatcagc tccgactccg ccagctccac gccgtcctcc gcagcgagct
      181 cgccgaccac ctcccacgcc cgccacatcg gggtcgtgcg ggcgttcagg agattcatcg
      241 agacctgcgc ccgggcggga tgcccgcgct cgggctcctc gacccggaag ccgttggcct
      301 ggacgcgcgg cagcccgccg cccgattcgc gaacccgtcg ggcgatgcgc ttggcgagct
      361 cgacatcgtc cgacgcgagg ttgatgttcc atgcgatcag gaacggccgc gccccgacgg
      421 ccacggcccc ggcccgcggg tgcatccgct ccggtccgta gtcgggctgc cggccgttgt
      481 gcgtgatctc gactttgaga ccctcgtact gcccgcgtcg gacgtcggcc agcctcacac
      541 ggtcggcgcg tgcggcggcc tcggcgtaga ggtacacggg caggtcccag cgggccgcga
      601 tccggcgccc gaacgcccgc gccatctcga cgcaagcgtc catcggcgtg tcggcgagcg
      661 gcacgaacgg gacgacgtcg acggcgccga tccgcgggtg ctcgcccgtg tgggtctcca
      721 tgtcgatctc gtcgagcgcc cggccgacca tctcctcgag ccccgcggcg accggctcgt
      781 gctcgccggc gatcgtgaac accgagcggt tgtggctggc gtcggaggtg cggtcgagga
      841 gaaacacgcc gggcgtgccg acgagggcgt cggcgaggcg atcgacgacg tcgagacgcc
      901 ggccctccga cacgttgggg acgctctcga ccagcacgcc gcgatcctac tcgcccgcgt
      961 gacgccccga cgggccgccc ggcgacgacg ccgcttgacc gtctcggcgg tggcaccgca
     1021 cgtacactcc ctcgtcaatg accgagccgc gccgaagcct cctccaccac cccgacttcc
     1081 tcaagttgtg gaccgccgag acgatctcgg tgttcggcac cgcagtcagc cagctcgccc
     1141 tgccgctgat cgcggcgacg atcctgcagg tcagcgcgtt cgagttcggc ctgctgacca
     1201 cgatcgcgtt cctgccgttc atcctgctga gcctgccggc gggggtgtgg gtcgaccggc
     1261 tccgccgccg gccgatcctg atcctcggcg acctcggccg cgcgatcgcg atcgcgtcca
     1321 tcccgatcgc cttcgccttc gatgcgctga cgatctggca gctgtatgtc gtcggcttcg
     1381 tcaacggctg cctgacggtg ttcttcgacg tcgcctacca gagctacctg ccgtcgatcg
     1441 tggagcgcga ccagcgggtc gagggcaact ccaagctgga gatcacgcgc tccgccgcgc
     1501 agatcctcgg tcccggtctg gcgggcgtcc tgatcggggc gttccgggcg ccgttcgcga
     1561 tggtgatcga tgccgtgagc tacctgtggt cggcggtgtt cgtgttcctg atccggcgac
     1621 ccgagccgcc cgtcgtgccg catgacgagg cggcgcacgg gcccaagccg tcgatgcgca
     1681 aggagatcgc catcggcctg cgctacgtga ccgggcaccg ctggctgcgg tcgatcgcgg
     1741 cgacgacggg gacagcgaac ttcttcggca acgtcatggg cgc
//
LOCUS       DBNHXO010000516         2375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4136762, whole genome shotgun sequence.
ACCESSION   DBNHXO010000516 DBNHXO010000000
VERSION     DBNHXO010000516.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4136762"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="AC162A_10405"
     CDS             complement(<1..965)
                     /locus_tag="AC162A_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5011297.1"
                     /translation="MSEIVAPVAAPIVPAGPIDPAEIRGRRQWPLPKQAVPGAPLLRV
                     EDLAVRFSLPAGSVKAVDGVSFGLNDGEALGIAGESGCGKTTTALSLVRLLPANGRIR
                     SGKIELFGIDLVPKTETQLARYRWREISIVFQGAMNALNPVRRVGDQIAEPIELRLGQ
                     PRDSSRKRAAELLDLVGIPKKRASAYPHELSGGMRQRAMIAMALACDPAIVIGDEPTT
                     ALDVMVQAQILQLLEQLRRDLGLSLMLITHDLSVIAETCDRVMVMYAGRIAEEGPVRR
                     VFTAPRHPYTQALLGAYPNIHADRRTLEVIPGMPPDLRNPPPGCR"
     gene            complement(962..1912)
                     /locus_tag="AC162A_10410"
     CDS             complement(962..1912)
                     /locus_tag="AC162A_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5011298.1"
                     /translation="MSVTGIAGTELGAGRWSGTGRRTRDFLGKLLSRPDGMIGVAILG
                     VVTLLALFPTLFVGPLETLLGATGTPLEPPSAEHLFGTDELGRDILNLTVHGARISLT
                     IGLMATVVTIVIGALIGIVAGFVGGAVDNVLMRLSDFFLVLPTVVLALILAPIMLDVI
                     GPQAELFGIRSTLLVVVIVIGLTSWATTARVIRSQVLSLKERMFVDRARVIGSGSGRI
                     MRRHILPNVVNLIVAQSVLTFATAVFTETTLAFIGLGDPAAPSWGQLLNQAQTAGAPG
                     LGAWWYIAPPAIAVVLVVLAFTLVGNALDDVLNPKMLGRR"
     gene            complement(1909..>2375)
                     /locus_tag="AC162A_10415"
     CDS             complement(1909..>2375)
                     /locus_tag="AC162A_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017272050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5011299.1"
                     /translation="GMTTPGGDKTPIEALFDLGRHLVLPLTAVALGLIGAYSILMRSS
                     IIETRSEDYITTARAKGLSDTRILNKHAFPNALLPMVTLIAINLGYVVAGAITAEIVF
                     NWPGLGTLTVQALDARDYPLLQAIFLLIAVSVVFANFAADIVYGYLDPRVRA"
BASE COUNT          377 a          813 c          858 g          327 t
ORIGIN      
        1 aaccgacagc cgggcggtgg gttgcgcagg tcgggcggca tgcccgggat gacctcgagc
       61 gtccgccggt cggcatggat gttggggtac gcgcccagca gcgcctgggt gtacgggtgg
      121 cgcggcgccg tgaacacccg gcgcaccggg ccttcctcgg cgatccgccc ggcgtacatg
      181 accatgaccc ggtcgcacgt ctcggcgatg accgagaggt cgtgggtgat cagcatcagc
      241 gacagtccca ggtcgcgccg cagctgctcc agcagctgca ggatctgggc ctggaccatg
      301 acgtcgagag cggtcgtcgg ctcgtcgccg atcacgatcg cggggtcgca tgccagcgcc
      361 atcgcgatca tcgcccgctg gcgcatgccg cccgacagct cgtgcgggta ggccgacgcc
      421 cgcttcttgg ggatcccgac caggtccagc agctccgccg ctcgcttgcg cgacgaatca
      481 cgtggctggc ccagccgaag ctcgatcggc tccgcgatct ggtcgcccac ccggcggacc
      541 gggttgagcg cgttcatcgc gccctggaac acgatcgaga tctcccgcca tcggtagcgg
      601 gccagctggg tctcggtctt gggcacgaga tcgatcccga acagctcgat cttgccgctg
      661 cggatccgcc cgttcgcggg caggagccgg acgagcgaca gcgcggtggt ggtcttgccg
      721 cagccggact cgcccgcgat gccgagcgcc tccccgtcgt tgaggccgaa gctgacgccg
      781 tccaccgcct tgacgctgcc cgccggcagg ctgaagcgca ccgccaggtc ctcgacccgc
      841 aacagcggcg cgcccggcac ggcctgcttg ggcagcggcc attggcggcg accgcggatc
      901 tcggcggggt cgatggggcc ggcggggacg atgggcgcgg ccacgggcgc gacgatctcg
      961 ctcatcggcg gcccagcatc ttcgggttca ggacgtcgtc gagcgcgttc ccgaccaggg
     1021 tgaacgccag gaccacgagc accacggcga tcgccggcgg ggcgatgtac caccacgcgc
     1081 cgagtcccgg cgcacccgcg gtctgggcct ggttgaggag ctggccccac gatggcgccg
     1141 ccgggtcgcc gaggccgatg aaggcgagcg tcgtctcggt gaacacggcg gtcgcgaacg
     1201 tcaggacgga ctgggcgacg atcaggttga cgacgttggg caggatgtgc cgccgcatga
     1261 tccgcccgga cccggatccg atgacgcgcg cccggtcgac gaacatccgc tccttgagcg
     1321 acaggacctg gctccggatg acgcgcgccg tcgtcgccca gctggtcagg ccgatgacga
     1381 tgacgaccac gagcagcgtc gaccggatgc cgaacagctc cgcctgggga ccgatgacgt
     1441 cgagcatgat cggcgcgagg atcagggcga gcaccacggt cgggagcacg aggaagaagt
     1501 ccgacagccg catcaggacg ttgtcgacgg ctccgccgac gaacccggcc acgatcccga
     1561 tcagcgcgcc gatgacgatc gtgaccacgg tcgccatcag gccgatcgtg agcgagatgc
     1621 gcgccccgtg gaccgtcagg ttgaggatgt cgcggccgag ctcgtccgtc ccgaacaggt
     1681 gctcggcgct gggcggctcg agcggcgtgc cggtcgcgcc cagcaacgtc tcgagcgggc
     1741 cgacgaacag cgtcgggaac agggcgagca gggtcaccac cccgaggatg gcaacgccga
     1801 tcatgccgtc gggtcggctg agcagcttgc ccaggaagtc gcgcgtgcgc cgaccggtcc
     1861 cgctccagcg ccccgcgccg agctcggttc cggcgatccc ggtgacgctc acgcccgcac
     1921 ccgcggatcg aggtagccgt acacgatgtc cgccgcgaag ttggcgaaca cgaccgacac
     1981 cgcgatgagc aggaagatcg cctgcagcag cggatagtct cgggcgtcca gcgcctgcac
     2041 ggtcagcgtc ccgaggcccg gccagttgaa cacgatctcg gccgtgatcg cgcccgcgac
     2101 gacgtaaccg aggttgatgg cgatcagcgt gaccatcggc agcagcgcgt tcggaaacgc
     2161 gtgcttgttg aggatccggg tgtccgacag gcccttcgcc cgggccgtgg tgatgtagtc
     2221 ctcggaacgc gtctcgatga tcgaggagcg catcaggatc gagtaggcgc cgatcagccc
     2281 gagggcgacg gccgtaagcg gcaggacgag gtggcggccg aggtcgaaca gggcctcgat
     2341 cggggtcttg tcgccaccgg gcgtggtcat tcccg
//
LOCUS       DBNHXO010000517         1847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1954370, whole genome shotgun sequence.
ACCESSION   DBNHXO010000517 DBNHXO010000000
VERSION     DBNHXO010000517.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1954370"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1068)
                     /locus_tag="AC162A_10420"
     CDS             complement(169..1068)
                     /locus_tag="AC162A_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011300.1"
                     /translation="MAKGKSNTSAAAAAAAEVGVEDLGAGAELIDAAGDAARETVALA
                     AEGAADVTSGLDSLDRAESLDALGDLATEEAIADTIRGAATLEAAEETADVGALVAAV
                     SADNLDRGMFLASLAGQLDVASNVVELMRMPVLSAFLDAKGRQLRGLAVNEIGRAMAA
                     AALAEGMQGLSARLAAIGLSEIAEGISEIEAADELLDARDDAVASGIESAAAGVAELS
                     AAEAGARATRQLAGAGVVMATQGAEEVGIAEGLASSAASSKAAAKPSAKKAAAAKAPA
                     KRPAKASAKTTAKRSSGGTTRKK"
     gene            complement(1227..>1847)
                     /locus_tag="AC162A_10425"
     CDS             complement(1227..>1847)
                     /locus_tag="AC162A_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR5011301.1"
                     /translation="ATSLHALPDEIDDTTGALVEPGGNAWRAIEAADVGAGERLLVLG
                     TGALGMLIALMARARDVEVHLVGRREGPMAFARSLDFERVWSASTLPDERFDAVIDAT
                     NGPELPALAVDVVEPGGRVVWIGLSGGPSLVDSRRVVLKDVTVVGILSASPGLAGALE
                     LFASGGVDPSPLVETVIGLDEVAGVLDGTRDPVFGEAPKVHVDPRR"
BASE COUNT          251 a          682 c          658 g          256 t
ORIGIN      
        1 ctccggggtc gtggatcccg cggcgtgcac ctgaacgtgg cgaccgcgaa cgagaacgcg
       61 atcgcgttct accgccacct cgggttcgag accctcgagg cgctcgacga ctcgctcgtg
      121 atggggatgc ggctggactg aggcccggcc cgagaggctg tcccgcggct acttcttgcg
      181 ggtcgtgccg cccgaagacc gcttggccgt cgtcttggcc gacgccttgg ccggacgctt
      241 cgccggggcc ttggccgccg ccgccttctt ggcagagggc ttggccgccg ccttggagct
      301 ggcggcactc gacgcaagac cctcggcgat gccgacttcc tcggccccct gggtggccat
      361 gaccacgccc gcgccggcga gctgacgggt cgcccgagcc ccggcctcgg ccgccgagag
      421 ctccgcgaca cccgcggccg ccgactcgat gccggacgcg accgcgtcgt cgcgcgcgtc
      481 gagcagctcg tcagcggcct cgatctccga gatgccctcg gcgatctcgg acagcccaat
      541 ggcggccagt cgggcgctca ggccctgcat gccctcggcc agcgcggccg cggccatcgc
      601 ccgcccgatc tcgttcacgg cgagcccgcg gagctggcgg cccttggcat cgaggaacgc
      661 ggagagcacg ggcattcgca tcagctcgac gacgttggac gccacgtcca gctgaccggc
      721 aagcgaggcc aggaacatgc cccggtcgag gttgtcggcg ctgaccgccg cgacgagtgc
      781 gccgacgtcg gcggtctcct ccgcggcctc gagcgtcgcc gcgccgcgaa tggtgtcggc
      841 gatggcttcc tcggtcgcga ggtcaccaag cgcatcgagc gactcggcgc ggtcgagcga
      901 atcgaggccg ctcgtcacat cggcggcgcc ctccgcggcc agcgccacgg tctcgcgagc
      961 ggcgtcaccc gccgcgtcga tgagctccgc acctgccccg agatcctcga ccccgacctc
     1021 ggcggcggcc gcggctgcgg ccgacgtgtt ggacttcccc ttggccatcg tgagctcctt
     1081 ctgccgtgtt cccggtcatc gtcgcgcgga ggcgccatcg atggaacgtc aactatgcgt
     1141 gattcggcgg cagcggggcg aggattgtgt gttaacgacc ggagtgatta ggcaccctgt
     1201 caccggaatc tgcgcgaggc tccgcgtcag cgacgggggt cgacgtggac cttgggggcc
     1261 tcgccgaaca cggggtcgcg cgtgccgtcg agcaccccgg cgacctcgtc gaggccgatg
     1321 accgtctcga ccagcggcga cgggtcgacg ccgcccgacg cgaacagctc gagggctccg
     1381 gcgaggcccg gcgatgcgct gaggatcccg accaccgtca cgtccttgag cacgacccgc
     1441 cggctgtcga cgaggctcgg gccgcccgag aggccaatcc acacgacgcg cccgcccggt
     1501 tccacgacgt cgacggcgag ggcgggcagc tcggggccgt tggtggcgtc gatgacggcg
     1561 tcaaagcgct cgtccggcag ggtcgaggcc gaccagacgc gctcgaagtc gagtgaccgc
     1621 gcgaacgcca ttgggccctc gcgccgcccg accaaatgca cctcgacgtc ccgggcacgg
     1681 gccatgagcg cgatgagcat gccgagcgca cccgtcccga ggaccagcag gcgctcgccc
     1741 gcgccgacat cggccgcctc gatcgcgcgc catgcgttgc caccgggctc gaccagggca
     1801 ccggtggtgt cgtcgatctc gtcggggagg gcgtggagcg aggtggc
//
LOCUS       DBNHXO010000518         4796 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3146251, whole genome shotgun sequence.
ACCESSION   DBNHXO010000518 DBNHXO010000000
VERSION     DBNHXO010000518.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3146251"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(603..1625)
                     /locus_tag="AC162A_10430"
     CDS             complement(603..1625)
                     /locus_tag="AC162A_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011302.1"
                     /translation="MLNGRWPRVTAAGVPLTELEADVAAGTVAVDALGAAIDDLVARV
                     VGVQVDAGLDVVTDGSVRFPDPGGALLRALAANDLGPQGYLVRTWQAAALLSDRVVAQ
                     PIPGPYSLSRRVHDDQSALQREDFTLEMASYLAAELRALAAAGCQMALIEEPDVVLIG
                     GYPAPNTNVIERELFAATQSHLLAEPVGLHAMLVVTGGSAWAAGPEAVLGAPYQSYLF
                     DLIAGPDNWNLIREVPGDRGVVCGALRAEAAADQLPELVWAARYAASSNGRGLERVGL
                     SNATSLADLDADAVGRAAAALAKASRLARLSPDQAVAEGLDARAIRQPDTRPPRVRRP
                     KPASGG"
     gene            complement(1637..2851)
                     /locus_tag="AC162A_10435"
     CDS             complement(1637..2851)
                     /locus_tag="AC162A_10435"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR5011303.1"
                     /translation="MTAQPPFDLRATLAQDGPLTRETALAGLDHASQLMTSADFIDAA
                     RLYQRIVGFNDVAITGAALVGLGEALHRLDDDWQALATWEEATRLPDNPATYPAWRNV
                     AAARVRADNLQGAIDAYREAEKRAPELDKAEIASRLGWLSKETGDKGAAGRYFARARG
                     EQGLSIAMVVLAVTVVVSLTCNFAGQAGIDLFNLLMLNKPLVAAGELWRLWTVALVHS
                     PLTEMPLHLVFNMYFLYLAGPFVERLYGRWTFLAFYLLFAVGGSLASFAFGSAALAVG
                     ASGAIFGLFGLLVAAERVHHPVLDRQSRGFLGQLTGLVVFNLLLGFIIPNVDNFAHIG
                     GLVTGLVMGFLFAPTRVPTLRSLWRKPGPTPGTSVPAFGNTGTLAIRGAGLVALGVAM
                     LVLWTIGVGVWG"
     gene            2938..3636
                     /locus_tag="AC162A_10440"
     CDS             2938..3636
                     /locus_tag="AC162A_10440"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HIR5011304.1"
                     /translation="MCGRFTQQRPTSEIASIFEANDLFDDPGGRFNVAPTDPAAVVVQ
                     RDDRRAVVGYRWGLIPGWADDPRIASRAFNARSESIATSPLFRDAYRRRRCLVPVDGF
                     YEWLRDGSRRQPVRIFDPDERPLALAGLWTGRQDPESGEWHRTFTIVTTRPNAFMTPI
                     HNRMPVVVPPESWAAWLDPTVRDPGEVRALLEPRDDIALDAYPVPPLVNNVRNNGPEL
                     VARVEPADAGLGLA"
     gene            complement(3633..4274)
                     /locus_tag="AC162A_10445"
     CDS             complement(3633..4274)
                     /locus_tag="AC162A_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR5011305.1"
                     /translation="MIVIVGGLAWRLGDEPAPAGRACEIALAAAALGARVEVVGRAGD
                     DPTGDAVMLGLARAGIGHVAVLRDPSRPTPVIEALAATDDEPGGDQSGPAPAAIGPAL
                     DAADVSLGLQYLTAFGVLVVTDDVPSAAIPVAIESAGFTGAHLILLTAADADPPDVSG
                     QATILAAPDEADDGAFARLVGTYAAGLDHGAEPARAFAAAVDASGWEALEPSV"
     gene            complement(4292..>4796)
                     /locus_tag="AC162A_10450"
     CDS             complement(4292..>4796)
                     /locus_tag="AC162A_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011306.1"
                     /translation="VGLAAGSGVATAGALGFMQGEAGVPSPQPSIDAAPTPDGNSVPS
                     MPPRTEEPTTQPPASVEPVAPDETPTEPADTPKPTDNADDHSGPGGGGSGDDNGGQRT
                     PKPTKESGGSGGGDDDGGDDGDHSGPGGGGDDDEDETETPEPTETPDDDSGHGGDSEH
                     GGGSGES"
BASE COUNT          662 a         1740 c         1779 g          615 t
ORIGIN      
        1 gtcgcgcgag acgccgggtt ctgcccgtga ccacgcggag ccgtgatagt cccaccgtgg
       61 cgcgtcggct gcaaccgcgt ttcgcaccgg ttgcgaggtg cggtgtcgcg agcggcccgt
      121 ggcgcccgtg tcgcggccga cgcgcagggg gcgggctccg ctcgcgttca tctcccgcca
      181 tcgagtcaga ggacgacttg tcgtcgatgg acgacggctc cagaggggcg gaaggcatcg
      241 gcagttagcc gccaacggca aaccatggga cgggacggcc accccctggc cagtcgagtg
      301 ccccgaatgc gcgccgaaca gtcgtccttt gactggctga cgggggagct ccccggcagg
      361 ctcgcgggtg gaacgcgaga cgttcactgc gtcggtcagg cgcgccttgg ggtctcgcta
      421 gggaaccgcg ggcgtgccag atggcgatcg ctccgccgaa cggctcgcct ggcgttccga
      481 atggcgccga cttgccgcgc cgatcgtcgc aacgaacgcc acatgttcca cgtcccgcgc
      541 tggccgagcg ctcccgggcc tggcctgtgg cgaacgcgcg ccgacccgcg agctgcgcgg
      601 cgctagccgc cgctggcggg cttgggacgg cgtacgcgcg gggggcgggt gtcgggttgg
      661 cggatcgcgc gcgcgtcgag gccctcggcg accgcctggt ctggcgacag ccgggcgagc
      721 cgactcgcct tggcgagtgc ggcggccgcc cgaccgacgg catcggcgtc gaggtccgcg
      781 agagaggtcg cgttgctgag gccgacgcgc tccagcccgc gcccgttcga cgacgccgcg
      841 tagcgcgcgg cccacacgag ttccggcaac tggtcggcgg cggcttcggc ccgcagcgcg
      901 ccgcacacca cgccgcggtc cccgggtacc tcgcggatga ggttccagtt gtcggggccg
      961 gcgatgaggt cgaacaggta cgactggtac ggcgcgccga ggaccgcctc ggggcctgcc
     1021 gcccaggccg agccgccggt gaccaccagc atggcgtgca ggccgacggg ttccgcgagc
     1081 aggtgcgact gggtcgccgc gaacagctcc cgctcgatca cgttggtatt cggcgccggg
     1141 tagccaccga tcagcacgac gtccggttcc tcgatcaggg ccatctggca gcccgcggca
     1201 gcgagcgccc ggagctcggc ggcgaggtac gacgccatct cgagcgtgaa gtcctcgcgc
     1261 tgcagggcgg actggtcgtc gtggacgcga cgcgacagcg agtacggccc cgggatcggc
     1321 tgcgccacga cccggtcgga caacagggcc gccgcctgcc acgtccgcac gaggtagccc
     1381 tgcggcccga ggtcgttggc ggcgagggca cgcaggaggg cccccccggg atcggggaag
     1441 cgcacgctcc cgtcggtgac gacgtcgagc ccggcgtcga cctgcacgcc gaccacacgc
     1501 gcgacgagat cgtcgatggc cgcgcccagc gcatcgactg ccacggtccc ggcggccacg
     1561 tcggcctcca gctccgtcag ggggaccccg gccgcggtga ccctcggcca tcgaccgttg
     1621 agcatcgaga acatcgctac ccccagaccc ccacaccgat ggtccacagc acgagcatcg
     1681 ccaccccgag cgccaccagg ccggccccgc ggatggccag ggtcccggtg ttgccgaagg
     1741 ccggcaccga ggtcccgggt gtcggtccgg gcttgcgcca cagggagcgc agcgtgggca
     1801 cccgcgtggg cgcgaacagg aagcccatca cgaggccggt caccaggccg ccgatgtggg
     1861 cgaagttgtc gacgttggga atgatgaacc cgagcagcag gttgaacacc acgagcccgg
     1921 tgagctggcc caggaagccg cggctctggc ggtcgaggac ggggtggtgc acccgctcgg
     1981 ccgcgacgag gaggccgaac aggccgaaga tcgcgcccga tgcaccgacc gcgagcgcgg
     2041 cggagccgaa cgcgaacgac gcgagcgagc cgcccacggc gaacagcagg tagaacgcga
     2101 ggaacgtcca gcgcccgtac agccgctcga cgaacgggcc cgccaggtac aggaagtaca
     2161 tgttgaacac gaggtggagc ggcatctccg tgagcggcga gtggaccagc gcgaccgtcc
     2221 acagccgcca cagctccccg gccgcgacca agggcttgtt gagcatcagg agattgaaca
     2281 ggtcgatgcc cgcctggccg gcgaagttgc aggtcagcga gacgacgacg gtcaccgcga
     2341 ggacgaccat cgcgatcgag agtccctgct ccccgcgggc acgggcgaag taccgcccgg
     2401 cggcaccctt gtcgccggtc tccttggaca gccagcccag ccgcgaggcg atctccgcct
     2461 tgtccagctc gggggcgcgc ttctcggcct cgcggtaggc gtcgatcgcc ccctggaggt
     2521 tgtcggcccg cacgcgcgcc gccgccacgt tgcgccaggc cgggtaggtt gcgggattgt
     2581 cgggcaggcg ggtcgcctct tcccaggttg cgagggcctg ccagtcgtcg tcgaggcggt
     2641 ggagggcctc gcccaggccg accagcgccg ccccggtgat ggcgacgtcg ttgaacccga
     2701 cgatccgctg gtacagccgg gcggcgtcga tgaagtccgc cgaggtcatc agctgcgacg
     2761 cgtgatcgag gccggcgagg gccgtctcgc gtgtcagcgg accgtcctgc gcgagggtgg
     2821 cgcggaggtc gaacggcggc tgcgcggtca cgggcgcgac tgtacaggag gcaggccgac
     2881 ccgggcgccg cgcagtggtc ggggttcggc ggaccggacg ggctagactc gcccggcatg
     2941 tgcgggcgct tcacccagca acggccgacg tcggagatcg cgagcatctt cgaggccaac
     3001 gacctgttcg acgacccggg tggccggttc aacgtggcgc ccacggaccc tgcggcggtg
     3061 gtcgtccagc gcgacgatcg ccgggcggtg gtcggctatc gctggggcct gatcccgggc
     3121 tgggcggacg atccgcggat cgcgtcgcgg gccttcaacg cgcggtcgga gtccatcgcc
     3181 accagccccc tgttccgcga cgcgtaccgt cgtcgccgat gcctcgtccc ggtcgacggc
     3241 ttctacgagt ggctccgcga cggaagccgc cgccagccgg tgcggatctt cgatcccgac
     3301 gagcggccac tggccctggc cggcctgtgg acggggcggc aggacccgga gtccggcgaa
     3361 tggcaccgga cgttcacgat cgtcacgacg cgcccgaacg cgttcatgac ccccatccac
     3421 aaccggatgc cggtcgtggt cccgcccgaa tcgtgggcgg cctggctcga tccgacggtg
     3481 cgtgacccgg gcgaggtgcg cgccctcctc gaaccccgcg acgacatcgc gctcgacgcc
     3541 taccccgtcc cgccgctcgt gaacaacgtc cgcaacaacg gcccggagct ggtcgcgagg
     3601 gtcgagccgg ccgacgccgg gctcggactc gcctagaccg aaggctcgag cgcctcccag
     3661 ccggaggcat ccacggcggc ggcgaacgcc cgggcgggct ccgccccatg atcgaggccg
     3721 gcggcgtagg tgccgaccag ccgggcgaac gcgccgtcgt cggcctcgtc aggtgccgcc
     3781 aggatggttg cctggccaga cacgtcgggc gggtcggcgt ccgcggccgt gagcaggatc
     3841 aggtgggcgc cggtgaagcc ggccgactcg atggcgacgg ggatggcggc tgacggcacg
     3901 tcgtcggtca cgacgaggac cccgaacgcg gtcaggtact ggaggccgag cgagacgtcg
     3961 gccgcatcga gcgcgggccc gatcgccgcg ggcgccggac cagattggtc cccgcccggc
     4021 tcgtcgtcgg tcgcggccag cgcctcgatc accggtgtgg gccgggacgg atcgcggagg
     4081 accgcgacgt gtccgatgcc cgcgcgcgcg agcccgagca tgacggcgtc acccgtgggg
     4141 tcgtcgccgg cgcgtccgac gacctcgacc cgcgccccga gggccgccgc ggcgagcgcg
     4201 atctcgcacg cgcgccccgc gggagccggc tcgtcgccca gacgccaggc gaggccgccg
     4261 acgatcacga tcacacggca agcctacccg gtcaggactc gccggatccg ccgccgtgct
     4321 ccgaatcgcc gccgtgaccc gagtcgtcgt cgggcgtctc ggtcggctcc ggcgtctcgg
     4381 tctcgtcctc gtcgtcgtcg ccaccgccgc caggcccgct gtgatcgccg tcgtcgcccc
     4441 cgtcgtcgtc accgccacca gacccgcccg actccttggt cggcttgggg gttcgctggc
     4501 cgccgttgtc gtcgccggag ccaccgccgc caggcccgct gtggtcatct gcgttgtcgg
     4561 tgggcttggg cgtgtcggcc ggctccgtcg gcgtctcgtc gggcgcgacg ggctccaccg
     4621 atgcaggggg ctgcgtcgtg ggttcttcgg tccgcggcgg catcgacggc accgagttcc
     4681 cgtccggcgt gggcgccgcg tcgatcgacg gttgcggcga cggcacgccg gcctcgccct
     4741 gcatgaaccc gagtgccccc gcggtcgcga cgccggaccc agcggccagc ccgacc
//
LOCUS       DBNHXO010000519         4717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_766256, whole genome shotgun sequence.
ACCESSION   DBNHXO010000519 DBNHXO010000000
VERSION     DBNHXO010000519.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_766256"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="AC162A_10455"
     CDS             complement(<1..1379)
                     /locus_tag="AC162A_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011307.1"
                     /translation="MGATGYRRWRRFAALLIAIVVATGCGSSAPTTTTAVPSSAVPAA
                     SAAATTDGGPGASVSIVSPVPQVDVPEPTLTDPAAIGEALRDPARAAEGVASMLAAAG
                     IGVYRQDGTALRPGLERSEHDPFVYDAEVLGLIEMAEADGAAIAAGGTPATVETLRAG
                     LAELLPDLTTDQLLAAIRASLDAHPAGLPAAVIGADLAADTPLTRAQLWFLYLHGFVG
                     IPSVTALRSRVASADVWAAPLVAPGAPLAGVPFIPSPEPGVDARDYALLLAHVQLAAW
                     LIPFQPSTYAASVHEGHGGPGTPLTLAAFHTPWYQSPVGAFSFRPLLLPSNGRLDGVP
                     VTWSSSDAGVLNAHGTLNPPLDVPIPTDFSGKASLTYTPRKESVATQSGPVETDTAQL
                     TASVPVRDLFMSHFELPVGAYGLLTGERQVPVVMAIEWHEEPGWKVDGASGGGRVTGQ
                     HCGDVAGDWV"
     gene            1606..3171
                     /locus_tag="AC162A_10460"
     CDS             1606..3171
                     /locus_tag="AC162A_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011308.1"
                     /translation="MTESTAASPSRRARAFTLYPFLFAAYPVLFLWSQNLGETSAGDV
                     LPLLGIVLAVTAVAMVVLTLIFRDARRAALVVAPIAVAILMYGHLAIIVRPFHVGPLL
                     QQAAWVLLVGLGILGALRLRERPLQRASGILNGISAILVIVALVGIVPYQVTAATTAQ
                     VAPPQAVAGSGASQRPLRDVYYLILDRYGSDEALSLRYGIENDLTPWLADHGFRVLND
                     SNANYVRTTLSIASTLNMTHLTDLAARVGADSRGYGPLYDMLQDSRIVEQFKALGYKY
                     THIGSYFSPTRSDTGADRDLYIGGPSDFGATLYDTSALPRVFRALHIAGLSRAERLYE
                     NKVFGFKALDSVRDDPGPKFVFAHILLPHPPYLITRDGQFRDAAAAAQLSDEEQFGDQ
                     LAWTNDQLRAWIESLQALPEDRRPIIILQADEGPYPRAYGADTTTYDWADASPRDLLI
                     KFGILNAWYLPDGTDPGLYDSMTSVNTFPTLFSGYYGLDVPNLEDRVYTSKRPGQPYD
                     LTDITDRLESAQP"
     gene            complement(3197..4624)
                     /locus_tag="AC162A_10465"
     CDS             complement(3197..4624)
                     /locus_tag="AC162A_10465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5011309.1"
                     /translation="MTAPPTAEREIEDSATPRIDRDPGSYRDPGGFVYRRDGVLLRQV
                     GESLIADWEAFVASPLATRLVEQGSLISWTPVGLDRAATSEARAVIAPEAVEFISYPY
                     EWTFGQLRDAALLTLDIQLEALDAGWTLRDASAYNIQFRAGRPVLIDLLSLEPLEPGQ
                     PWVAYRQFCEQFLAPLALMARRDIRLSRLLRADPEGVPLDLAARLLPARSRLNFGLLT
                     HLYLHAAAQGRYAGNEDDGAAAKRTKIDPVRLRALIGSLRKTVAGLRWEPKGTEWADY
                     AGNTSYSDTATAAKERLVADYVGRIDGPVVWDLGANTGRYSRIAADAGKRVLAFDIDP
                     AAAERNYRALTGEARSDILPLVLDLANPSPGIGWAGRERRSLLERADADGTLALALVH
                     HLAISRNVPLPMFAALLADLAPGAVVEFVPKEDRMVRRLLAARRDVFPDYTLDGFRDA
                     VRTRFEIVAESPIEDSGRTLFLLRR"
BASE COUNT          691 a         1676 c         1691 g          659 t
ORIGIN      
        1 acccagtcgc ccgccacgtc gccgcagtgc tggccggtga cccgaccgcc gccggacgcg
       61 ccgtcgacct tccagcccgg ctcctcgtgc cactcgatcg ccatgaccac cgggacctgg
      121 cgctcccccg tcaagaggcc ataggcgccg acgggcagct caaagtggct catgaacagg
      181 tcccggacgg gcacggacgc ggtcagctgc gcggtgtccg tctcgacggg tccggattgc
      241 gtggcgactg actccttgcg cggcgtgtac gtcagggacg ccttgccgct gaagtccgtc
      301 gggatgggga cgtcgagcgg tgggttcagg gtgccgtgcg cgttgagcac gcctgcgtcg
      361 gacgacgacc aggtgaccgg gacgccgtcc aggcgcccgt tggatggcag cagaagcggg
      421 cggaacgaga aggccccgac cggcgactgg taccacggcg tgtggaaggc ggcgagggtc
      481 aggggcgtgc ccgggccgcc gtgcccctcg tggacgctgg cggcgtacgt gctgggctgg
      541 aacgggatga gccaggcggc gagctggacg tgggccagga ggagcgcgta gtcgcgggcg
      601 tcgacacccg gctcgggcga cgggatgaac gggacgcccg cgagcggtgc gccgggtgcg
      661 acgagcggcg cggcccaaac atcggccgac gcgaccctcg agcggagcgc ggtgacggac
      721 gggatcccca cgaacccgtg cagatacagg aaccacagct gggcgcgcgt cagcggtgtg
      781 tcggcggcga ggtccgcgcc gatgacggcg gctgggagcc cggccgggtg ggcgtcgagt
      841 gacgcgcgaa tcgccgcgag cagctggtcg gtggtcaggt ccggcaagag ctccgctagc
      901 ccagcccgga gcgtctcaac cgtggccggc gtgccccccg cggcgatcgc cgcgccgtct
      961 gcctccgcca tctcgatcag cccgaggacc tcggcgtcgt agacgaacgg gtcgtgctca
     1021 gagcgctcca accccggccg gagcgcagtt ccgtcctggc ggtagacgcc gatccccgcc
     1081 gcggcaagca tcgatgccac gccttccgcg gcccgcgccg gatcccgcag ggcctccccg
     1141 atcgccgccg gatcggtgag cgtcggctcc gggacgtcga cttgtggaac cggcgagacg
     1201 atcgagacgc tcgcgccggg cccgccgtcg gtggtcgctg ccgccgacgc tgccggcacg
     1261 gcactcgagg gcacggccgt ggtcgtcgtc ggcgcggacg acccgcagcc cgtggccacg
     1321 acgatggcga tcaaaagggc ggcgaaccgc cgccatcgcc ggtacccggt cgcacccacg
     1381 cgacccctcc tcgcgtccgt ctgggcgggt gtcggatgac ccgcctcgga gaccggatca
     1441 tgcgcctgca ggcgggcccg gctcatcgtc cagttgcgcc acctcggaag gacgcgaccc
     1501 tggccaccgg ctccggccca cttcggcatc cagcggtatg atctggcgcg ctgcggcttg
     1561 gagggcgttc tcgagcagcc cagcgaatgg gagcccgcac cccgcgtgac cgaatccacg
     1621 gccgcctcgc cgtcacgacg cgcccgcgcg tttacgctgt atcccttcct gttcgccgcg
     1681 tatccggtgc tgttcctgtg gtctcagaac ctgggcgaga cgagtgccgg cgacgtgctg
     1741 ccgctgctgg gcatcgtgct ggccgtgacc gccgtggcga tggttgtgct caccctgatc
     1801 ttccgcgacg cgaggcgggc cgcgctcgtg gtggcgccga tcgccgtcgc gatcctgatg
     1861 tacggccacc tcgcgatcat cgtgcggccg ttccacgtgg ggccgctgct ccagcaggcc
     1921 gcctgggtcc tgcttgtcgg gctcggcatt ctcggtgcgc tgcggctgcg cgagcgaccg
     1981 ctgcaacgcg cgagcgggat cctcaatgga atcagcgcga tcctcgtgat cgtggcgctg
     2041 gtggggatcg tcccctacca ggtcacggcc gccaccacgg cgcaggtggc cccaccccag
     2101 gccgtggccg gatccggcgc atcgcagcgt ccgttgcgcg acgtctacta tctgatcctc
     2161 gaccgctacg gatcggacga ggccctgtcg ctccggtacg ggatcgagaa cgacctcacg
     2221 ccgtggctcg ccgatcacgg gttccgggtg ctgaacgaca gcaacgcgaa ctacgtcagg
     2281 acgacgctgt cgatcgcgtc gacgctcaac atgacccatc tcaccgacct cgccgcccgc
     2341 gtgggcgcgg acagccgagg gtacgggccg ctgtacgaca tgctccagga ctcgcgcatc
     2401 gtcgaacagt tcaaggcgct cggctacaag tacacgcaca tcgggagcta cttcagcccg
     2461 acgcgtagcg acaccggcgc ggaccgcgac ctgtacatcg gtgggccgtc cgacttcggc
     2521 gccacgctct acgacacgag cgcgctgccc cgggtgttcc gtgccctcca catcgcgggc
     2581 ttgtcccgcg cggagcgcct gtacgagaac aaggtgttcg gtttcaaggc gctcgactcg
     2641 gtgcgggacg atccgggccc gaagttcgtc ttcgcgcaca tcctgctccc ccaccccccg
     2701 tatctgatca cgcgcgacgg gcagttccgg gacgcggccg ccgccgcaca gctcagcgac
     2761 gaggagcagt tcggcgacca gctcgcgtgg acgaacgacc aactgcgcgc ctggatcgaa
     2821 tcgctccagg ccctccccga ggaccgacgc ccgatcatca tcctgcaggc cgacgagggc
     2881 ccctatcccc gcgcatacgg ggccgacacg acgacgtacg actgggcgga cgcgtcgcca
     2941 cgcgacctgc tgatcaagtt cggcatcctg aacgcctggt acctgcccga cggcaccgac
     3001 cccggcctgt acgactcgat gaccagcgtc aacacgttcc cgacgttgtt ctcgggctac
     3061 tacgggctgg acgtcccgaa tctcgaggac cgcgtctaca cctcgaagag accgggccag
     3121 ccatacgacc tcacggacat cacggaccgc ctcgagtccg cccagccctg acggcggccg
     3181 gcgtgtgctc gcgcgatcag cgccggagca ggaacagcgt ccgaccgctg tcctcgatgg
     3241 gcgactcggc cacgatctcg aaccgcgtcc gaacggcgtc cctgaacccg tcgagggtgt
     3301 agtccgggaa cacgtcgcgg cgcgcggcaa ggagccggcg caccatccgg tcctccttgg
     3361 gcacgaactc cacgacggcc cccggcgcga ggtcggcgag cagcgccgcg aacatcggca
     3421 gcggcacgtt gcgcgagatc gcaaggtggt ggacgagggc gagcgcgagc gtcccgtcgg
     3481 cgtccgcccg ctcgagcagc gaccggcgct cgcggcccgc ccagccgatg cccgggctcg
     3541 ggttggcgag gtcgaggacc agcggcagga tgtccgaccg ggcctcgccg gtgagcgcgc
     3601 ggtagttgcg ctccgcagcg gccggatcga tgtcgaacgc cagcacgcgc ttgccggcat
     3661 cggcggcgat ccggctgtag cgcccggtgt tggcgcccag gtcccacacg accgggccgt
     3721 cgatccgccc gacgtagtcg gcgaccagcc gctccttcgc cgcggtggcg gtgtcgctgt
     3781 agctcgtgtt gccggcgtag tcggcccact cggtgccctt gggttcccag cgcagccccg
     3841 cgacggtctt gcgcaggctg ccgatcaagg cgcgcagccg gaccgggtcg atcttcgtgc
     3901 gcttcgcagc ggcgccgtcg tcctcgttgc ccgcgtagcg gccctgagcg gccgcgtgga
     3961 ggtacaggtg tgtgaggagc ccgaagttga ggcgtgaccg cgcgggcagg agtcgggcgg
     4021 cgaggtcgag cgggacgccc tccgggtcgg cccgcaacaa cctcgagagc cggatgtcgc
     4081 ggcgtgccat gagcgcgagc ggcgcgagga actgctcgca gaactggcgg tacgcgaccc
     4141 acggctggcc gggctcgagg ggctccagcg agaggaggtc gatgagcacc ggacggccgg
     4201 cacggaactg gatgttgtag gcggacgcgt cgcgcagcgt ccagcccgcg tccagcgcct
     4261 cgagctggat gtcgagggtg agcagcgcgg cgtcgcggag ctggccgaac gtccactcgt
     4321 aggggtagct gatgaactcg accgcctcgg gcgcgatgac cgcgcgcgcc tcggaggtgg
     4381 cggcccggtc gaggcctacg ggcgtccagg agatgagcga gccctgctcg acgagccggg
     4441 tagcgagcgg gctggcgacg aacgcctccc aatcggcgat cagcgactcg ccgacctggc
     4501 gcaggagcac cccgtcgcgg cgatagacga accccccggg gtcgcggtag gagcccggat
     4561 cgcgatcgat gcgcggtgtc gcggagtcct cgatctcgcg ctcagccgtg ggcggtgctg
     4621 tcatcgaccc cgtcggccgg ctcgtggtct cgccggatca ggcgggccag ctgggcgatt
     4681 cgcgcccgaa agatcaccgg gatcgcgatg atcgacg
//
LOCUS       DBNHXO010000520         3822 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3941875, whole genome shotgun sequence.
ACCESSION   DBNHXO010000520 DBNHXO010000000
VERSION     DBNHXO010000520.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3941875"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="AC162A_10470"
     CDS             <1..964
                     /locus_tag="AC162A_10470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR5011310.1"
                     /translation="VVSLDPVAKTVSMASIPRDMVDVPLPDGRKYRGKINGLVSYARW
                     HPNKFPGSKDGQSVLAAALGTLLDMKINYWAQVNLGGFVYLVDSVGGVNLTVTDGFCD
                     YRYKEYGIRGFNITPGRYHMDGEAALAYARVRKAAGESDFTRAGRQQEVVAALRDRLV
                     RGEFLGNPSKFLKSLGQTILTNIKPSVIADYLAIATQVKRNDVFRDVFDHPLVKSGYD
                     ARGSIQIPDVKAIRRKAARLFPPTGTRPSEARFDPLPEPGEGPLRSPSKSSTCGIAPN
                     PKPKPTPKPTPKPTPKPTKKPPKATPEPTPEPSPSEEAESQPNP"
     gene            966..1505
                     /locus_tag="AC162A_10475"
     CDS             966..1505
                     /locus_tag="AC162A_10475"
                     /inference="COORDINATES: protein motif:HMM:NF025052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5011311.1"
                     /translation="MALEIPDPCLVVLVGAAGSGKSTFAARHFAPDEVLSSDAFRELI
                     AGDPADQTATHAAFSALHKALARRLFEGRLTVVDATSVQRGARAALLRRSAAAGVPAV
                     AVVLALPPDVVQARNRERPPGAIVPAEAVDRQLAALATALRPDGMDAEGFARIHVLTT
                     PADVDAAVVLRVPGQRGPA"
     gene            1801..2931
                     /locus_tag="AC162A_10480"
     CDS             1801..2931
                     /locus_tag="AC162A_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5011312.1"
                     /translation="MRPVTRGRSLATLAFAAAIVAACTSGGGAASPTGAAATPAAATP
                     AGSDAASPEGGVAPLSDAVTVRFGVFPNITHAPGLVALADGGPLETLLPNADVQITPF
                     NSGTSAVEAMFADALDITYIGPNPAINAFAKSNGEAVRVISGSTSGGAFLVVKPEIAS
                     AADLRGKKIASPSLGNTQDVALRSWLKEQGLSTDTAGGGDVSVVPQENAQTLETFQAG
                     TIDGAWVPEPWATRLIQEAGAKVLVDERDLWPEGKYVTTHLMVAKKFLDANPEVVKRI
                     LAANIEAVDEVNADPAAAQATVNTEIEKFTTKKLGTDLLAAAWKNLVFTVDPIASSLG
                     KSATDAQALGLLKDPGDLSKLYDLTLLNELLQASDEQPVKGL"
     gene            2931..3749
                     /locus_tag="AC162A_10485"
     CDS             2931..3749
                     /locus_tag="AC162A_10485"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5011313.1"
                     /translation="MGSTAIAEILPQPTATPPPPGSATARGEAVRIDHVTKVFSGGGD
                     AVTALEDISLSVGRGEFVCLLGASGCGKSTLLNLVARLDSPTAGSVECVGDPSFMFQE
                     AALFPWLNVERNVELPLRLRKVPKAQRKERVEELLELVQLSAFAKRQPHELSGGMRQR
                     VALARSLAQEAEVLLMDEPFGALDAMTRDILHDELERIWQATGRTILFVTHNVREAVR
                     LGDRVVLLTSRPGKVAAEFPVDMRRPRRIEDRDVAQLAAEITTRLREEVARHGR"
BASE COUNT          601 a         1439 c         1235 g          547 t
ORIGIN      
        1 ggtggtctcg ctcgacccgg tcgcgaagac ggtatcgatg gcctcgatcc cccgcgacat
       61 ggtcgacgtg ccgctgccgg acggccgcaa gtaccgcggc aagatcaacg gcctcgtcag
      121 ctatgcccgg tggcacccca acaagttccc gggctcgaag gacgggcagt cggtgctggc
      181 tgcggcgctg gggacgctgc tcgacatgaa gatcaattac tgggcgcagg tcaacctcgg
      241 cggtttcgtg tacctcgtcg actcggtggg cggcgtcaac ctcaccgtca ccgacggctt
      301 ctgcgactac cgctacaagg aatacggaat caggggcttc aacatcaccc ctggcaggta
      361 ccacatggac ggcgaggcgg cgctcgccta cgcgcgcgtt cgcaaggccg ccggcgagag
      421 cgacttcacc cgcgcggggc ggcagcagga ggtggtcgcg gcgctccggg accggctggt
      481 tcgtggcgag ttcctcggca atccgtcgaa gttcctcaag tcgctggggc agacgatcct
      541 caccaacatc aaaccctccg tgatcgccga ctacctcgcg attgcgaccc aggtcaagcg
      601 caacgacgtc ttccgcgacg tgttcgacca cccgctggtg aagtcgggct acgacgcccg
      661 gggctcgatc cagatcccgg acgtcaaggc catccggcgc aaggcggcgc ggttgttccc
      721 gccgaccggc acccggccca gcgaagcgcg cttcgatccc ctccccgagc ccggcgaagg
      781 cccgctccgc agcccctcga agtcgtcgac ctgcggcatc gctcccaacc ccaagcccaa
      841 gccgaccccc aaaccaaccc cgaagccgac gcccaagccg accaagaagc cgcccaaggc
      901 gacgcccgag ccgacgccgg agccatcgcc ttcggaagag gccgagtcgc agcccaaccc
      961 ctgacgtggc gctcgagatc cccgacccct gcctggtcgt gctggtgggg gccgccggat
     1021 ccggcaagag caccttcgcc gcgcggcact tcgcgccaga tgaggtcctc tcgtccgacg
     1081 cgtttcgcga gctgatcgcg ggcgacccgg ccgatcagac ggcgacgcac gccgcgttct
     1141 ccgcgctgca caaggccctc gcgcgccggc tcttcgaggg aaggctcacg gtcgttgacg
     1201 cgacgagcgt gcagcgcggc gcgcgtgctg ccctgctccg ccggtccgcc gcggctggcg
     1261 ttccggccgt tgccgtcgtc cttgccctcc cgcccgacgt cgtccaggct cgcaaccgcg
     1321 aacggccgcc cggcgccatc gtcccggccg aggcagtgga ccggcagctc gccgccctgg
     1381 ccacggccct gcgtccagac gggatggatg cggagggctt cgcccggatc catgtgctca
     1441 cgacgccggc cgacgtcgat gcggcggtcg tgcttcgggt cccggggcaa cgcggacctg
     1501 cctgacggcc ccgactgctc gcccagcgag cagtgcagcg gttggtccgc tggatcgctc
     1561 gcccagcgag caggttgacg gttgtgtggc ccgccatccg ctgaacggct cgctcagcga
     1621 gccatcgagc tctgtatccg ctggattgct cgcctggcga gcacccggcg gggtccggac
     1681 gttgctgcgt gctgatttga caaccccgca caccctgtgg tctattctca ctagttcggt
     1741 agaaataacc cgaaaacacg acatcacacc agaccctgca gcaacgcaac cggaggtctc
     1801 atgcggcccg tcacacgcgg acgatccctc gccaccctcg ccttcgccgc cgccatcgtc
     1861 gcggcgtgca ccagcggcgg cggtgcggcc agcccgacgg gtgccgctgc cacgcccgcc
     1921 gctgccacgc ccgccgggtc cgacgcggcg tcccccgagg gcggcgtcgc accgctgtcc
     1981 gacgcggtta ccgtccggtt cggcgtgttc cccaacatca cccacgcgcc cggcctcgtc
     2041 gccctggccg acggcggtcc gctcgagaca ctgctcccga acgccgacgt ccagatcacc
     2101 ccgttcaact cgggcacgtc ggcggtcgag gcgatgttcg ccgatgcgct cgacatcacc
     2161 tacatcggcc ccaacccggc gatcaacgcg ttcgccaagt cgaacggcga ggcggtccgg
     2221 gtcatctccg gctcgacctc gggcggcgcc ttcctcgtgg tcaagcccga gatcgccagc
     2281 gcggcggacc tgcggggcaa gaagatcgcc agcccctcac tcggcaacac ccaggacgtc
     2341 gcgctccgat cctggctcaa ggaacagggc ctctccaccg acaccgccgg cggcggcgac
     2401 gtctcggtcg tgccccagga gaacgcgcag acgctcgaga cgttccaggc cggcaccatc
     2461 gacggcgcct gggtccccga gccgtgggcg acccggctga tccaggaggc cggcgccaag
     2521 gtgctcgtcg acgagcgtga cctgtggccc gagggcaagt acgtcaccac ccacctgatg
     2581 gtcgccaaga aattcctcga cgccaacccc gaggtcgtga agcgcatcct cgcggccaac
     2641 atcgaggcgg tcgacgaggt gaacgccgat cccgcagccg cccaggcgac ggtcaacacc
     2701 gagatcgaga agttcacgac caagaagctc ggcaccgatc tcctcgccgc ggcctggaag
     2761 aacctcgtgt tcacggtcga cccgatcgcg tcgtcactcg ggaagtcggc gacggacgct
     2821 caggccctcg ggctgctcaa ggacccgggc gacctctcga agctgtacga cctgacgctg
     2881 ctcaacgagc tcctccaggc gtcggacgag cagcccgtca agggcctctg atgggatcga
     2941 cggcgatcgc ggaaatcctt ccgcagccga cggctactcc tcctcccccc gggtcggcaa
     3001 cggcccgggg ggaagccgtt cggatcgacc acgtgacgaa ggtgttctcg ggtggcggtg
     3061 acgcagtcac ggccctcgag gacatttcgc tgtccgtggg ccgcggcgag ttcgtgtgcc
     3121 tcctcggagc ctcgggatgc ggcaagtcga cgctgctcaa cctcgtcgcc cgcctcgaca
     3181 gccccaccgc cggcagcgtc gagtgcgtcg gcgacccgtc gttcatgttc caggaggcgg
     3241 ccctgttccc gtggctcaac gtggagcgca acgtcgagct gccgctccgc ctgcgcaagg
     3301 tgcccaaggc gcagcgcaag gagcgggtcg aggaactgct ggagctcgtc cagctgagcg
     3361 cgttcgcgaa gcgccaaccc catgagctct cgggcggcat gcgccagcgc gtcgccctcg
     3421 cgcgctcgct cgcccaggag gccgaggtcc tgctcatgga cgagccgttc ggggcactcg
     3481 atgccatgac ccgcgacatc ctccacgacg agctcgagcg gatctggcag gccaccggcc
     3541 gcacgatcct gtttgtgacc cacaacgtgc gcgaggccgt gcgcctcggg gaccgggtcg
     3601 ttctgctgac cagccgcccc ggcaaggtcg ccgccgagtt cccggtggac atgcggaggc
     3661 cgcgccggat cgaggatcgc gacgtcgccc agcttgccgc cgagatcacc acgcggctcc
     3721 gcgaggaggt cgcacgccat ggacgctagt cttcggggcg tgctccccgt caaacagccc
     3781 tcgccccagc ccgctgcccc gggactccct gcgccccgtc gc
//
LOCUS       DBNHXO010000521         4303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2750384, whole genome shotgun sequence.
ACCESSION   DBNHXO010000521 DBNHXO010000000
VERSION     DBNHXO010000521.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2750384"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="AC162A_10490"
     CDS             <1..585
                     /locus_tag="AC162A_10490"
                     /inference="COORDINATES: protein motif:HMM:NF015102.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HIR5011314.1"
                     /translation="EMDLTVERGVGYLAAERSEALPIGVIAVDAIFTPVRKVNYWVES
                     MRVGQMTNYDKLTIEIETDGTITPEEALSRSADTLVRQFQPFVTIGVQQAAPDRAAAG
                     VPQLPPNMLDMPIEELDLPMRAYNSLKRNNIVKVGQLLQLKDDDLLRMRNFGKKSLDE
                     MKERLRMRGFILPESETDGVLDGDDEPFGDEDEV"
     gene            589..942
                     /gene="rplQ"
                     /locus_tag="AC162A_10495"
     CDS             589..942
                     /gene="rplQ"
                     /locus_tag="AC162A_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR5011315.1"
                     /translation="MAHRIDGRKLSRKHGPRMALYKNLTVSVLRYERIQTTEAKAKEI
                     RGQVERMITLAKRGDLAARRTVVSELPNEPLVVSKLFEVIAPKYADRQSGFTRLVHIG
                     QRKGDAAEIVQIELV"
     gene            991..1749
                     /gene="truA"
                     /locus_tag="AC162A_10500"
     CDS             991..1749
                     /gene="truA"
                     /locus_tag="AC162A_10500"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR5011316.1"
                     /translation="MRYRATVEYDGTDFAGFQLQPNARTVQGELEAALTRLERGVRVV
                     VDGAGRTDTGVHATGQVIGFTYRGGLTEEALRQAVNALLPPDVAIRGVRRAPARFHPR
                     YAARYREYRYTVWNGPRSPLRERQALGMRSPLNIAAMASAASVFEGRHDFSSFGGTDR
                     SPVRTVHSVRVRRDGRLVTIDVRADSFLRGQVRRMVAALLEVGLGTLDTDGLRAALAG
                     PGPALNGASAPAKGLCLRRVVLGGRPSDDEEDEE"
     gene            1746..2177
                     /gene="rplM"
                     /locus_tag="AC162A_10505"
     CDS             1746..2177
                     /gene="rplM"
                     /locus_tag="AC162A_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR5011317.1"
                     /translation="MNAKTYTPRESEIERRWYVVDATDETLGRLATRIARVLEGKHKP
                     TYAAHLDSGDHVIVLNAARITVTSDKLTSKTYARHSGYPAGFKEETLGHLLARRPEEV
                     IRRAVKGMLPHTTLGTQMLRKLKVYAGNDHPHQAQKPEPLA"
     gene            2191..2589
                     /gene="rpsI"
                     /locus_tag="AC162A_10510"
     CDS             2191..2589
                     /gene="rpsI"
                     /locus_tag="AC162A_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR5011318.1"
                     /translation="MSTPAFYWGTGRRKTAVARVRLIPGEGEMVVNGRTPEEHFGGVL
                     AESELFLPFRVTGTEGRYNTMIKVEGGGITGQAGAIRHGIARALLQVDAETNRLPLRQ
                     AGLLTRDPRMKERKKYGLKRARKAPQYTKR"
     gene            2911..3879
                     /gene="argF"
                     /locus_tag="AC162A_10515"
     CDS             2911..3879
                     /gene="argF"
                     /locus_tag="AC162A_10515"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007778557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR5011319.1"
                     /translation="MTSESLRGRDFISAADLTADEVGQVFARATALKAEFAEDRRHAR
                     PPLERRTLAMLFQRPSLRTRVTFEAAMTQLGGHAIYLTEGVVLGGRESVADVAHNLER
                     FVDGIMARTGPHEVVVELAAQASIPVVNGLTIREHPCQALADLFTLHERFGDLRGLPL
                     AFVGDGNNVYHSLALLGATLGMEVRIAHPAGYGPNERLVARAAEIAAASGGRLVFDTD
                     PRAVVQGAAVIYTDAWTSMGQEAEAEERRDAFARYQVNGDLLAMAPDALVMHCLPAHR
                     GEEITSDVMDGPRSIIFDQSENRLHTQKALLVEVIGDEPPDGRQAT"
     gene            3876..>4303
                     /locus_tag="AC162A_10520"
     CDS             3876..>4303
                     /locus_tag="AC162A_10520"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5011320.1"
                     /translation="MSGEALYERYKDALRRGHVASLRGRLEEALTAYAEAAAIAPERA
                     TPHTSAGTALMRRKRPADAVRHYTTAIRLSPHDEAALLGRAQALTAMNRRPQAADAYD
                     AAADALAASGKLADAVDAARRGLELAEGRERRRTLEQLIER"
BASE COUNT          711 a         1506 c         1519 g          567 t
ORIGIN      
        1 gagatggacc tcaccgtcga gcgcggcgtg ggctacctcg ccgccgagcg ctccgaggcc
       61 ctgccgatcg gcgtgatcgc ggtcgacgcg atcttcacgc ccgtgcgcaa ggtcaactac
      121 tgggtcgaga gcatgcgggt cggtcagatg accaactacg acaagctcac gatcgagatc
      181 gagaccgacg gcacgatcac ccccgaggag gccctgagcc ggtcggcgga cacgctggtg
      241 cgccagttcc agccgttcgt gacgatcggt gtccagcagg ctgccccgga ccgcgccgcc
      301 gccggcgtcc cgcagctccc gccgaacatg ctcgacatgc cgatcgagga gctggacctg
      361 ccgatgcggg cctacaactc gctcaagcgc aacaacatcg tcaaggtcgg ccagctgctg
      421 cagctcaagg acgacgacct gctgcgcatg cgcaacttcg gcaagaagtc gctcgacgag
      481 atgaaggagc gcctccgcat gcgcggcttc atcctccccg agtccgagac cgacggcgtc
      541 ctcgacggcg acgacgagcc gttcggcgac gaagacgagg tctgagcgat ggcgcaccgc
      601 atcgacggcc gcaagctctc gcgcaagcac ggaccgcgca tggccctgta caagaacctg
      661 acggtgtccg tgctccgcta cgagcggatc cagaccaccg aggccaaggc caaggagatc
      721 cgcgggcagg tggagcggat gatcacgctc gccaagcgcg gtgacctggc cgcccgacgg
      781 acggtcgtct cggagctccc gaacgagccg ctcgtcgtga gcaagctgtt cgaagtcatc
      841 gcccccaagt acgccgatcg ccagtcgggc ttcacccggc tggttcatat cggccagcga
      901 aaaggcgatg ccgccgagat cgttcagatc gaactggtct gagcgacgca gagaccgaat
      961 gacatagcag ggttccgaga gggcccgccg atgcgctacc gcgcgacggt tgaatacgac
     1021 ggaacggact ttgccgggtt tcagctccag ccgaacgccc ggacagtcca gggagagctc
     1081 gaggccgcgc tcacccgcct cgaacgaggc gtgcgggtcg tggtcgacgg ggcgggacgc
     1141 accgataccg gtgtccacgc cacgggtcag gtgatcggtt tcacctaccg tggggggctc
     1201 accgaggagg cgctccggca ggcggtcaac gccctgctcc cgccggacgt cgcgatccgc
     1261 ggcgtgcgtc gggccccggc gaggttccac cctcgctacg cggcgcggta tcgggagtac
     1321 cgctacaccg tctggaacgg gccgcgcagc ccgcttcgcg agcgccaggc gctcgggatg
     1381 cggagcccgc tcaacatcgc cgccatggcg agcgccgcgt cggtcttcga aggccggcac
     1441 gacttctcga gcttcggcgg cacggaccgg tcgccggtcc ggaccgttca ttcggtccgg
     1501 gtccgacggg acggccgcct ggtgacgatc gacgtccggg cggactcgtt cctccggggc
     1561 caggtgcggc gcatggtcgc cgccctcctg gaggtcggac tgggaacatt ggacaccgat
     1621 ggcctcaggg cggcgcttgc cggtccgggg ccggcactca acggggcatc ggcccccgcc
     1681 aagggcctgt gcctacggcg cgtcgtcctc ggagggcgac cgtcggacga cgaggaagac
     1741 gaggaatgaa cgcgaagacc tatacgcctc gagagagcga gatcgagcga cgctggtacg
     1801 tcgtggacgc gacggacgag acgctcggcc ggctcgcgac gcggattgcg cgtgtcctcg
     1861 aggggaagca caagccgacc tatgccgctc acctcgactc gggcgaccac gtgatcgtgc
     1921 tcaatgcggc gcggatcacc gtgaccagcg acaagctcac ctccaagacc tacgcccgcc
     1981 acagcggcta ccccgcggga ttcaaggagg agaccctcgg tcacctgctc gcccgccggc
     2041 cggaggaagt catccgccgc gccgtcaagg ggatgctgcc gcacaccacg cttggaaccc
     2101 agatgctccg gaagctcaag gtctatgcgg gaaacgatca tccgcatcag gcccagaagc
     2161 ccgagccgct ggcctgatcg aggagccctg atgtcgacgc ctgcgttcta ctggggcacc
     2221 ggccgccgca agacggcggt ggcccgcgtc cgcctcattc ccggtgaagg cgagatggtc
     2281 gtcaacggcc gcacgcccga ggagcacttc ggtggcgtgc tcgccgagtc cgagctgttc
     2341 ctgccgttcc gggtgaccgg gaccgagggc cgctacaaca cgatgatcaa ggtcgagggt
     2401 ggcgggatca ccggccaggc cggcgcgatc cgtcacggca tcgcgcgcgc cctgctgcag
     2461 gtcgatgccg agaccaatcg cctcccgctg cgccaggccg gcctcctgac ccgcgacccg
     2521 cggatgaagg agcgcaagaa gtacggcctc aagcgcgcga gaaaagcccc gcagtacacc
     2581 aagcggtaac gcaccgcggc ccggcggccg atcgcggcgt ttcccgctcc gggcgctcgc
     2641 tcgctgatgg atcgggtccg aataccgccc cgagcggtgc ggggcgggga cctgggttca
     2701 tataccgccg ccagtggtag gtgagcgtca gctcaccctc ggcggtgccg ttcgagcctc
     2761 tcgcacaccg ccaccggcgg tatccggacc tcgcagccgc gccgcggtgc cgacaaccgg
     2821 gcgcggcggt caccgacacg ccgcacgtcg agcgacgggc cgtgaacggg atggccgcga
     2881 gtcgtgctcg gtgctccgta cactgggcac gtgacgagcg agagcctccg ggggcgggac
     2941 ttcatctccg cggctgacct gacggccgac gaggtcggac aggtgttcgc ccgtgcgacg
     3001 gcgctcaagg ccgaatttgc cgaggaccgg cgccacgcgc ggccgccgct cgagcggcgc
     3061 accctggcca tgctgttcca gcgcccgagc ctccgcacgc gggtgacgtt cgaggccgcg
     3121 atgacccagc tgggcgggca tgcgatctac ctgaccgagg gcgtcgtgct cggcggccgg
     3181 gagagtgtgg ccgacgtggc gcacaacctc gagcggttcg tggacgggat catggcccgg
     3241 accgggccgc acgaggtcgt ggtcgagctc gcggcccagg cgtcgatccc cgtcgtcaac
     3301 gggctcacca ttcgtgagca cccgtgccag gcgctcgccg acctgttcac gctccacgag
     3361 cggttcgggg acctgcgcgg gctcccactg gcgttcgtgg gtgacggcaa caacgtctac
     3421 cactcgctgg ccctgctggg cgcgacgctg ggcatggagg tccggatcgc gcatccggcc
     3481 ggctacgggc ccaacgaacg gctcgtcgcc cgggccgccg agatcgccgc cgcgtcgggg
     3541 ggccggctcg tgttcgacac cgatccccgg gccgtcgtcc agggggccgc cgtgatttac
     3601 acggacgcct ggacctcgat gggccaggag gccgaggcgg aggagcgccg cgacgccttc
     3661 gcgaggtacc aggtcaacgg cgacttgttg gcgatggcgc ccgacgcgct cgtgatgcac
     3721 tgtctgcccg cgcaccgcgg cgaggagatc acgtcggacg tgatggacgg accgcggagc
     3781 atcatcttcg accagtcaga gaaccgcctc cacacgcaga aggcgctgct ggtcgaggtc
     3841 atcggcgacg agccacccga cgggaggcag gcgacatgag cggtgaggcc ctgtacgagc
     3901 gctacaagga cgcgctccgc cggggccacg tcgcgtcgct gcgggggagg ctcgaggagg
     3961 ccctgaccgc ctacgcggag gccgccgcca tcgcgcccga gcgggccacg ccacacacga
     4021 gcgccggcac cgcgctgatg cgtcgaaagc gccccgcaga cgccgttcgc cactacacga
     4081 ccgcgatccg cctgtcaccg cacgacgagg ccgcgctgct gggccgcgcg caggcgctga
     4141 cggcgatgaa ccgacgaccg caggccgcgg acgcgtacga cgcggcggcc gacgcgctgg
     4201 ccgcgtcggg caagctggcc gacgcggtgg atgccgctcg gcgcgggctc gagttggccg
     4261 agggtcgcga gcgacgacgg acgctggagc agctgatcga acg
//
LOCUS       DBNHXO010000522         2002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1956420, whole genome shotgun sequence.
ACCESSION   DBNHXO010000522 DBNHXO010000000
VERSION     DBNHXO010000522.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1956420"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1224
                     /locus_tag="AC162A_10525"
     CDS             136..1224
                     /locus_tag="AC162A_10525"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HIR5011321.1"
                     /translation="MTLTIELRTLVLPLRDPFVIARSSHGEGRSVTTVVAELRDDADG
                     PDGPVGLGEGFPDAYYGETPETMIAVMPRLLAAVEPMAAALRGGRDEARAALDGAADR
                     MADSIEHHGAAKCAIDIALHDLVGKRLGLPIRDLLGIEGPIPPTDFTLGIDEPAVVAE
                     RARRAGDFPALKLKVGGPSDLETVRAVRAVFEGPLRVDANTGWTPYGAVDLLPELERL
                     GVELIEQPFPARDHADLRELQARSPLPLVADESAVTIDDLDPLVGVVDGVNVKLAKCG
                     GIGPAHRMLVRARQLGFRTFLGCMEETSIGIAASAAVAPLADWVDLDGCLLLADDPVT
                     GLELGTDKRWILSEAPGLGLHWRHPTAR"
BASE COUNT          268 a          713 c          751 g          270 t
ORIGIN      
        1 gaccgccgcc gagaccggcc ggcggtgcga cgacccggcc cgcttggggg gcgccgcgct
       61 gtggcgtgac atcgtggcgg cggtcgacgc cctcccgtgg gtgacgccgg ccgagccggc
      121 cgaggaggct cccgcatgac gctgacgatc gagctgcgga cgctcgtgct gccactgcgt
      181 gacccgttcg tgatcgcgcg gtcgtcccac ggcgagggca gaagcgtgac gaccgtcgtc
      241 gccgagctgc gcgacgacgc cgacgggccg gacggaccgg tcggcctggg cgagggcttc
      301 cccgatgcct actacggcga gacgcccgag acgatgatcg cggtgatgcc gcggctgctg
      361 gccgccgtgg agccgatggc ggcggcactt cgcggcggcc gcgacgaggc tcgggcggca
      421 ctcgatggcg cggcagaccg gatggcggac tcgatcgagc atcacggggc ggccaagtgc
      481 gcgatcgaca tcgcgctcca cgacctcgtg ggcaagcggc tcggcctccc gatccgtgac
      541 ctgctcggga tcgagggccc gatcccgccg accgacttca ccctcggcat cgacgagccg
      601 gcggtcgtgg cggagcgggc gcgccgcgcc ggtgacttcc ctgcgctcaa gctcaaggtg
      661 ggcggaccga gcgatctcga gaccgtgcgg gccgtgcggg ccgtgttcga gggaccactg
      721 cgcgtcgacg ccaacaccgg ctggacgccg tatggcgcgg tcgacctgct gccggagctc
      781 gaacgcctgg gcgtcgagct gatcgagcag ccgttcccgg cccgcgacca cgccgacctg
      841 cgcgagctcc aggctcgatc cccgctgccg ctcgtcgcgg acgagagcgc cgtgacgatc
      901 gacgacctcg acccgctggt gggcgtcgtc gacggcgtca acgtcaagct cgcgaagtgc
      961 ggcggcatcg gccccgccca ccggatgctc gtgcgcgcgc ggcaactcgg cttccggacg
     1021 ttcctcggct gcatggagga gacctcgatc ggcatcgccg cgtcggccgc ggtcgcgccg
     1081 ctcgccgact gggtcgacct agacggctgc ctgctgctcg ccgacgaccc ggtgacaggt
     1141 ctcgagctgg gcaccgacaa gcgctggatc ctctccgagg ctcccgggct cggtctccac
     1201 tggcgtcatc cgaccgcccg ctgaggcacg ccgtccacag gtttccgcga cttgttgggg
     1261 cctgtccacc gagggttgcg gggtgtggac aagtcgggga ggaacatggt ggagaagccc
     1321 ctaccgggca aacgccacag gacctacgat gtgccgatcg ccggtgaccg cggacggccc
     1381 gagggaggga caccggcgag agcaagcaga cacccccggc ggcgggcggc cccggctcgc
     1441 cgccggttga ccacgcacgc ccctgggaga ggaggaccta tctcgatgct gcccgctggc
     1501 accgccgccc gcccgtcgcc ggaggccgag gccttcgtgc gattctgcta tcagcgccgg
     1561 cgcgtggggt ggcccgagct gtatgacgag atgtgcgccg tcgcgacgcg cggcctgttc
     1621 cggggcatgg gccatgaggc gctggcggag atcggggtgg ggttcagcct gttccagacg
     1681 ccgcggctgg ccgagctggt cacccagatc gtgaccgccg agcaggccgc gaggcgggcg
     1741 gcacgggcgg cgatgatcga ggccgccgag gcggcgcgcg tcgatggggg cgagcgatcg
     1801 gacgcccagc cggccgcgga tcctgggctg gccgtcgaga cccggcagca actccctgcg
     1861 gcgaccgccg cctatacccg gcctaggccc ggatctgccg ccgcgccatc tcgccctcga
     1921 tctggcgctc ggcctccacg tagcgatcga tctgctcgcg aagctcgttt gcctcggggg
     1981 cgtagaggtg gacctgctgc tg
//
LOCUS       DBNHXO010000523         5296 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2950150, whole genome shotgun sequence.
ACCESSION   DBNHXO010000523 DBNHXO010000000
VERSION     DBNHXO010000523.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2950150"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="AC162A_10530"
     CDS             <1..345
                     /locus_tag="AC162A_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5011322.1"
                     /translation="IVGGIGIMNIMLVSVRERTREIGIRKAVGARRRDILAQFLIEAL
                     TLSGLGGLIGIVLGLTVSGLIARLGGITFAFSPTTLAVAFLFSLAVGVVFGVWPARQA
                     ARLDPISALRYE"
     gene            359..1099
                     /locus_tag="AC162A_10535"
     CDS             359..1099
                     /locus_tag="AC162A_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011323.1"
                     /translation="MTTPHEPANLSADASPTEPVPPMPAFAPAQSATAFQPAQPEPMP
                     VGTPVVATGSAIAPTPPVAKPARKSGSVSILNVALGVAVLVATAGVAFAVGRSTAPAA
                     APTQVAGVTPGGRFFTNGGPGASLDPNGGFGAGPGANSGNGNGNGGRFGFAGGPTIEG
                     TVDSITADSVTIKTADGNTITIGLDSATTYHQQADATASDVEAGKTVILKLSGGFRPG
                     NGNGNGNGNGNGNGSVSLGTASDVTVVP"
     gene            1119..1796
                     /locus_tag="AC162A_10540"
     CDS             1119..1796
                     /locus_tag="AC162A_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5011324.1"
                     /translation="MHLLLVEDDERLARALTRLLEEDRHIVELAPDGQTGIDLARAIE
                     GLDAIILDIGLPDMSGLDVARRLRRDKIEVSILMLTARDTVNDRVSGLDAGADDYVVK
                     PFAYQEIAARLRALARRAEAGPRRPEPRLEVGVITLDEAARRVTVNGRNVDLSPREFS
                     LLEALMRHPGQTLTRDQLLDHAWPFSVAVTPNAVDAYVHYLRTKLGDAGSQVETVRGV
                     GYRLGDG"
     gene            1789..3225
                     /locus_tag="AC162A_10545"
     CDS             1789..3225
                     /locus_tag="AC162A_10545"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5011325.1"
                     /translation="MADGRRLDPEDDGAEQAALVDEAKLIRRVRWRLVAWSGISTLLI
                     LVVLGAALYAAVANSLAAASVTQLQNRAHPLVELLQGPTGEPGDGPDQGFVFGGGNTV
                     LYIFDDQGNPVQVGDRPVPVPEGLPIEGGVEAAKAAPNGTDVRTATLELGAQQALTVP
                     VRVMTVGVTAQQDGNTYYLQVLQDRSTEVETLQALLTVLLVGGLLVVIVAVAFGWIYA
                     ERALVPIRNSLGAQRTSLRRQREFAADASHELRTPLTVIRSSLEHVFRHPDRPVGESR
                     EALDDIDAEVTHLTAMVDDLLLLARSDSGAVSIERTQVDLGDVAFDAASAFGRTAEER
                     GVRVEVDPAPAMIDGDPARLRQLVTILVDNAVRHSPRGSSVTVSVRAVGPTNVIEVAD
                     EGPGLREEDMAHVFDRFYRAPGAPTGGTGLGLAIGRWIVDRHGGRIGVANREPHGAVF
                     RVELPTVAPVAHPPAVTATDPQLPPAAT"
     gene            3261..3776
                     /locus_tag="AC162A_10550"
     CDS             3261..3776
                     /locus_tag="AC162A_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007379310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative immunity protein"
                     /protein_id="HIR5011326.1"
                     /translation="MILPEVRDPRLVTVRRGGTLQDDDHRLLAVWAADCAQHVLHHFE
                     RARPGDDRPRRAIELGRAWARGEITMTHARSAGGHANAAARDLSGAARHAAFAAGQAG
                     VVAHVAAHELGAAAYAIKAVWAASPAGERREAGRRECQWQRARLPTAIRELVLDDQRL
                     RNKLCWFAFDC"
     gene            3980..5143
                     /gene="carA"
                     /locus_tag="AC162A_10555"
     CDS             3980..5143
                     /gene="carA"
                     /locus_tag="AC162A_10555"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR5011327.1"
                     /translation="MIVPPTVRQPPLPRADALVDPRIGDAGPALLALEDGTVFPGIAF
                     GAPVSAGGDLVVNTSQTGYQEIATDPSYAGQVVVMTYPLIGNYGRLRDEDQSERPWLR
                     GLVVAHATAAVLDDARQLATMLRRAGIPAIAGVDTRALARHLRSSGCLRGVITEPGIG
                     DRHQAVALARAVPRWEDQDFVAQVSPSEVREVGDEAGEPLVAIVDYGLKANIVNSLRR
                     RGVRVRVLPHTASAAEVMAGDVSGVVFSPGPGDPARLDGPVALARSVIEDGRPLLGIC
                     LGHQIVARAAGADTTRLRFGHHGANHPVQDLDTGYVQVTAQNHEVQVVGGSLPASSGF
                     RVSQINLNDRSVEGLRHAERPIETVQYHPEGAPGPLDALAVFDRFVEACRRGA"
     gene            5140..>5296
                     /locus_tag="AC162A_10560"
     CDS             5140..>5296
                     /locus_tag="AC162A_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017599990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase preATP-grasp
                     domain-containing protein"
                     /protein_id="HIR5011328.1"
                     /translation="MTDAGRVRRPKPASVLILGSGPVVIGQAAEFDYAGTQACRALRA
                     EGVRTVLV"
BASE COUNT          703 a         1983 c         1878 g          732 t
ORIGIN      
        1 atcgtgggcg gcatcgggat catgaacatc atgctggtct cggtccgcga gcggacccgc
       61 gagatcggca tccgcaaggc ggtcggtgcc cggcgtcgcg acatcctcgc gcagttcctg
      121 atcgaggccc tgacgctgtc gggcctcggc gggctgatcg gcatcgtgct cggcctcacc
      181 gtgtccgggc tcatcgcacg cctgggcggg atcaccttcg ctttcagccc cacgacgctc
      241 gccgtcgcgt tcctgttcag cctcgccgtc ggcgtcgtgt tcggcgtctg gccggcgcgc
      301 caggcggccc gcctcgaccc catcagcgcg ctgcgctacg agtaaggaga taccccgcat
      361 gaccacgccc cacgagcccg cgaacctctc ggccgacgcg tccccgacgg agcccgtgcc
      421 gccgatgccg gcgttcgcgc cggcgcagtc cgcgaccgcg ttccagccgg cacagcccga
      481 gccgatgccg gtcggcaccc ccgtcgtggc caccggctcg gccatcgcgc ccacgccgcc
      541 cgtcgccaag cccgcccgca agtcgggatc cgtgtccatc ctcaacgtcg cgctcggcgt
      601 cgcggtgctg gttgcgacgg ccggggttgc gttcgcggtc ggtcgctcga cggcccccgc
      661 cgccgcgccg acgcaggttg ccggcgtcac gcccggcggc cgcttcttca ccaacggggg
      721 tcccggcgcg agcctcgacc ccaacggcgg cttcggggcc gggcccggcg ccaacagcgg
      781 caatggcaat ggcaacggcg gccgcttcgg gttcgccggt ggcccgacga tcgagggcac
      841 cgtcgactcg atcacggctg actcggtgac gatcaagacc gccgatggca acacgatcac
      901 catcggcctc gattccgcga ccacgtacca tcagcaggcc gacgcgacgg cgtcggacgt
      961 cgaggccggc aagaccgtga tcctgaagct ctcgggcggc ttccgacccg gcaacgggaa
     1021 cggaaatggg aatggcaatg ggaacggcaa cggctccgtc agcctcggca ccgccagcga
     1081 cgtgaccgtc gtcccctgag cgaggacccg aggagaccat gcacctgctc ctggtcgagg
     1141 atgacgagcg cctcgcccgc gcgctgaccc ggctgctcga ggaagaccgg cacatcgtcg
     1201 agctcgcgcc cgacggccag accggcatcg atctcgcacg ggcgatcgag ggcctggacg
     1261 cgatcatcct cgacatcggg ctgccggaca tgtccggcct cgacgtggcg cgccgcctgc
     1321 ggcgcgacaa gatcgaggtg agcatcctga tgctcacggc acgcgacacc gtcaacgacc
     1381 gggtctccgg cctggacgcc ggcgccgacg actacgtcgt caagccgttc gcctaccagg
     1441 agatcgcggc acggctgcgt gcgctcgcac gtcgcgcgga ggccgggccg aggcgcccgg
     1501 agcctcgcct cgaggtcggc gtcatcaccc tcgacgaggc cgcccggcgg gtcaccgtca
     1561 acggccgcaa cgtcgaccta agtccgcgcg agttttcgct gctcgaggcg ctgatgcgcc
     1621 acccgggcca gacgctgacg cgcgaccagc tgctcgacca cgcctggccg ttcagcgtcg
     1681 cggtgacgcc caacgccgtc gacgcctacg tccactacct gcgcacgaag ctgggcgacg
     1741 cgggctcgca ggtggagacg gtgcgcggcg tgggctaccg gctcggcgat ggctgacgga
     1801 cgccggctcg acccggagga cgatggggcg gagcaggcgg ccctcgtcga cgaggcgaag
     1861 ctcatccgcc gtgtccgctg gcgccttgtc gcgtggagcg gcatctcgac gctgctgatc
     1921 ctggtcgtcc tcggcgctgc cctctacgcc gcggtcgcca actcgctggc cgcagcgtcc
     1981 gtgacgcagc tccagaatcg agcccacccg ctggtcgagc tgctacaggg gccgaccgga
     2041 gagcccggcg atggccccga ccaggggttc gtgttcggcg gcgggaacac cgtcctgtac
     2101 atcttcgacg accaggggaa cccggtgcag gtcggcgacc gaccggtccc cgtgccggag
     2161 ggtctgccga tcgagggcgg cgtcgaggcg gcgaaggctg caccgaacgg caccgatgtg
     2221 cggacggcga ccctcgagct cggcgcccag caagcgctca cggtccccgt tcgcgtgatg
     2281 accgtcggcg tcacggcgca gcaggacggg aacacgtact acctccaggt cctccaggat
     2341 cgctcgacgg aggtcgagac gctccaggcg ctgctgaccg tcctcctcgt cggcggcctg
     2401 ctggtcgtga tcgtcgccgt cgcattcggc tggatctacg ccgagcgcgc gctggtgccg
     2461 atccggaact ccctcggcgc gcagcggacg tcgctccgcc ggcagcgcga gttcgcggcg
     2521 gacgccagcc atgagctgcg gacgccgctg accgtgatcc gctcgtcgct cgagcacgtg
     2581 ttccgtcacc cggaccggcc ggtgggggag tcgcgcgagg cgctcgacga catcgatgcc
     2641 gaggtcaccc acctcaccgc gatggtcgac gacctgctgc tgctcgcccg ctccgactcg
     2701 ggggcggtgt cgatcgagcg gacgcaggtc gacctgggcg acgtggcctt cgacgcggca
     2761 tcggcgttcg gtcgcacggc cgaggagcgc ggcgtgcggg tggaggtgga tcctgcaccg
     2821 gcgatgatcg acggcgaccc ggcgcggctg cgccagctgg tcacgatcct ggtcgacaac
     2881 gcggtgcggc acagccctcg tggcagctcg gtgaccgtca gcgtgcgcgc ggtcggcccg
     2941 accaacgtca tcgaggtcgc cgacgagggc cccggcctgc gcgaggagga catggcccac
     3001 gtgttcgacc gcttctaccg cgcaccgggc gcgccgaccg gcggcacggg ccttgggctc
     3061 gcgatcgggc gctggatcgt ggaccgccac ggcggccgga tcggcgtcgc gaaccgcgag
     3121 ccgcacggtg cggtgttccg cgtggagctc ccgacggttg ccccggtggc gcacccgccc
     3181 gccgtgaccg cgacggatcc gcagctgccg cctgcggcga cgtaggtgcg cgggcggccc
     3241 gtcacacgga gcgacggctg atgatcctcc ccgaggtccg ggatccgagg ctcgtgaccg
     3301 tgcgtcgcgg cggcaccctc caggacgacg atcaccgcct cctcgccgtg tgggcggccg
     3361 actgcgcgca gcacgtgctc caccacttcg agcgcgcacg cccgggagac gatcgtccgc
     3421 gccgggccat cgaactgggg cgtgcctggg cgcgaggtga gatcacgatg acgcacgcac
     3481 gctcggcagg gggccatgcg aacgccgcgg ccagggacct gtccggagct gcgcggcacg
     3541 cggccttcgc cgccggccag gccggggtgg tcgcccacgt cgcagcccac gagctcggtg
     3601 cggccgccta tgcgatcaag gcggtctggg cggcgtctcc cgcgggcgag cgtcgcgagg
     3661 ccggtcgccg ggagtgccag tggcagcgcg cgcggcttcc caccgcgatc cgcgagctcg
     3721 tcctcgacga ccagcggctt cgcaacaagc tgtgctggtt cgccttcgac tgctgacggg
     3781 cttcattggc agccgtcgtt ccccggatcc gggcgccggt tcacgaagct cccggtaccg
     3841 ccgggatgct atcgtcaggg cgtcctgatg gccggtcctg agaggccggt gtaccggtcc
     3901 tgagaggccg ggtaggaggc cagatgctcg tgcgcacgcc cgcacgcggc gcgagttcgc
     3961 cgcggcccgc cgggatcccg tgatcgtccc accgaccgtc cgtcagcctc cgctgccgcg
     4021 cgccgacgcg ctggtcgatc cccgcatcgg cgacgccggt cccgcgctcc tcgcgctcga
     4081 ggacggcacc gtcttcccgg gcatcgcgtt cggtgccccc gtctccgcgg gtggcgatct
     4141 cgtcgtcaac acctcccaga ccggctacca ggagatcgcg accgatccct cgtacgccgg
     4201 ccaggtcgtg gtcatgacct acccgctgat cggcaactac gggcggctgc gcgacgagga
     4261 ccagtccgag cgcccgtggc tccggggcct tgtcgtcgcc cacgcgacgg ccgcggtcct
     4321 cgacgatgcc cgccagctcg cgacgatgct ccgccgggcc gggatcccgg ccatcgccgg
     4381 cgtcgacacc cgcgcgctcg cccggcacct ccggtcgagc ggctgtctgc gcggcgtgat
     4441 caccgagccc ggcatcggcg accgacatca agccgtcgcc ctcgcccgcg ccgtcccgcg
     4501 ctgggaggac caggacttcg tcgcccaggt ctcaccgtcc gaggtccgcg aggtgggcga
     4561 cgaggcgggc gagccgctgg tcgcgatcgt cgactacggg ctcaaggcga acatcgtgaa
     4621 ctccctgcgt cgacgcggcg tccgcgtccg ggtcctgccg catacggctt cggccgccga
     4681 ggtcatggcg ggcgatgtca gcggcgtcgt cttctcgccc ggccccgggg atccggcccg
     4741 gctcgacggg ccggtggcgc tggcccggtc cgtgatcgag gacgggcggc cgctgctcgg
     4801 gatctgcctc gggcaccaga tcgtggcccg cgccgccggt gccgacacca cgcgcctccg
     4861 gttcggtcat cacggcgcca atcacccggt ccaggacctc gacaccggct atgtccaggt
     4921 gaccgcgcag aaccatgaag tccaggtggt cggcggctcg ctgcccgcgt cgtcgggctt
     4981 ccgggtcagc cagatcaacc tcaacgaccg ctcggtcgag ggcctgcggc acgcagagag
     5041 gccgatcgag accgtccagt accaccccga gggcgcgccg gggccgctcg acgcgctggc
     5101 cgtgttcgac cggttcgtcg aagcctgccg gcggggcgca tgaccgacgc cggccgggtc
     5161 cgccgtccga agccggcgtc cgtgctgatc ctcggcagcg gccccgtcgt catcgggcag
     5221 gcggccgagt tcgactacgc cggcacccag gcctgccgcg ccctgcgcgc ggagggcgtc
     5281 cggacggtcc tcgtga
//
LOCUS       DBNHXO010000524         5316 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_373979, whole genome shotgun sequence.
ACCESSION   DBNHXO010000524 DBNHXO010000000
VERSION     DBNHXO010000524.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_373979"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="AC162A_10565"
     CDS             <1..230
                     /locus_tag="AC162A_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR5011329.1"
                     /translation="VVEVFRQTQALQAATFNFTPFLATALIFLVVTIPLARFTDYLLA
                     REKRRRSGGGGGGGGTRSMEQPGIRGMGGVS"
     gene            239..982
                     /locus_tag="AC162A_10570"
     CDS             239..982
                     /locus_tag="AC162A_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR5011330.1"
                     /translation="MPALSIEGLHKAFGSLDVLRGIDLDVAEHEVVALIGASGSGKST
                     LLRCVNLIEPIDAGRIRIKGEEITAKGVKVDRIRRRIGIVYQAYNLFPHMSVMDNVTL
                     APRKVLGRSRADAERDATELLTRFGLADKARDYPDSLSGGQQQRVAIVRALAMGPDLL
                     LLDEVTSALDPELVAEVLNVVRELAAGGMTMVIATHEMGFARDIANRVCFLDAGRILE
                     QGSPAQIFSNPQEPRTRQFLNRIIEAGRL"
     gene            complement(995..1756)
                     /locus_tag="AC162A_10575"
     CDS             complement(995..1756)
                     /locus_tag="AC162A_10575"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR5011331.1"
                     /translation="MSKAADGAPRIVVTLMVAAEQAEPGIAARKNALYLDALTRHGAV
                     TMPLDATSPEAERETAFAAMDGLLLSGGADVDPARYGQAVDGAHGVERERDALEAAAW
                     SAAEARAVPVFGICRGLQAMNVFAGGRLRQDIGGHAGHGWGHGDALTHPLRAIPGTRL
                     TRILYPANVGGGVLTVNSYHHQGVARDDLAPRFVASAVSPGPDGELVEAFESTAGPFR
                     MAVQCHPERTESTPRAFERLFAFFVDACRGPAASR"
     gene            complement(1781..2296)
                     /locus_tag="AC162A_10580"
     CDS             complement(1781..2296)
                     /locus_tag="AC162A_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR5011332.1"
                     /translation="MIRIHRRAHPRQGALPVVIGALALAIAIAGCLPASVRPTTPPPP
                     TPGPTPTAPPTPTPTPGPPTPTPGPTFRLYKVQRGDTLTRVARRFKTSTRSLSYWNRE
                     KYPTLDPESARYRPDRLELGWVLQVIPNKEYVAPEDDGETGIEVTPTPDDEEFDASPD
                     PSGSPVPSASP"
     gene            complement(2369..5260)
                     /locus_tag="AC162A_10585"
     CDS             complement(2369..5260)
                     /locus_tag="AC162A_10585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5011333.1"
                     /translation="MLAKSHIPATGTRVQRPGDATLSVTKAAHVLGVHPNTIRAWSEA
                     GRLRYYRINERGDRRYRLVDLQRFLTAAASDTPAGIADASQPTRIGRRSMDMADRTLT
                     ESAAGVDLLADLAEIASFPSGVDAALDQACHRIRVATGAALVGIWEVRPGGLVSRATD
                     VEGPGITAGRNVPPGRSLFSQALESPEPIHARPGVDGPEPLLGMGTDELVVRIPGGEH
                     PWGVLVIAGAVELGRDDGHHLARAIARTMGVLIQGANASEQASGRLRRAEALRRVATD
                     LTSRLDVSDVVRDLSDHARVLFGADRVAVVLHDTEGRVSSPGGTGFSDAFLDAARTLE
                     IDRLGQRELPSRRPVILVGPDTPRSGSPLRAAAIQEGVDTLLSAPLVDGHEIHGMLYL
                     AHDRPHRWRQIDLDSAEALAGDAAIAVRSARTFGRMATWAAQLQSIQRLGSRLSGLTD
                     VREIGHAIATELRQLIDYQNVRVYRVQGKDLMPVAMMGHGTVYSDETVESLRVEIGEG
                     VTGWVAKYRVPQLVDDTANDPRAITIPGSEPDIDESMLLAPMVHEGVCLGVLVLSKLG
                     LRQFTEDDLRLLVIYASFAAQAMTNADATAMMREQSSALERQLAAQRELLRTTESILT
                     TLDQRAVLEQITDRMGGLIHCDNIAIEVVDRSAGLLVPLTARGIHSDEYMQPWEPGEE
                     GIATWVVEHNEPVLIEDERTDERVNHFRATGAIDGSLIVVPLLGPEGAVGVMTLERLG
                     NATAFDEQEFELVKLFAAQVSIALRNAETFQAAEFRARTDDLTGLLNHRTFKDWLARS
                     VAGGETFGLVMVDLDEFKAVNDKLGHQAGDRLLREIAEALRAAARDTDAVFRYGGDEF
                     VLILPLSDPNGLLAVAERIRGAVAGVGGEGTNWRAENVVVAASIGTASFPVDGSTAED
                     VLLAADRACFVAKRGGRGLVATASEGLALASEFTLSEPTPVDPPSVPAD"
BASE COUNT          711 a         1879 c         1969 g          757 t
ORIGIN      
        1 gcgtcgtcga ggtgttccgc cagacccagg ccctccaggc cgccacgttc aacttcacgc
       61 ccttcctcgc gacggcgctg atcttcctgg tggtcacgat cccgctcgcc cggttcaccg
      121 actacctgct cgcccgcgag aagcgccgtc gcagcggggg cgggggcggc ggcggcggga
      181 cgcggtcgat ggagcagccg gggatccgcg gcatgggggg cgtcagctag tggacggggt
      241 gcccgccctg tcgatcgagg ggctccacaa ggcgttcggc agtctcgatg tcttgcgggg
      301 gatcgacctc gacgtcgcgg agcacgaggt cgtcgcgctc atcggggcgt cggggagcgg
      361 caagtccacg ctcctgcggt gtgtcaacct gatcgagccg atcgacgccg ggcgcatccg
      421 catcaagggc gaggagatca ccgccaaggg cgtcaaggtc gaccggatcc ggcgccggat
      481 cggcatcgtg taccaggcct acaacctgtt cccgcacatg tcggtgatgg acaacgtgac
      541 cctcgcgccg cgcaaggtcc tggggcgctc gcgggcggac gccgagcgcg acgcgaccga
      601 gctcctgacc cgcttcggcc tggcggacaa ggcgcgggac taccccgaca gcctgtcggg
      661 cggccagcag cagcgggtcg cgatcgtgcg cgcgttggcg atgggccccg acctcctgct
      721 cctcgacgaa gtcacctctg ccctcgaccc ggagctcgtc gccgaggtgc tgaacgtcgt
      781 ccgcgagctg gccgcgggcg gcatgacgat ggtcatcgcc acccacgaga tgggtttcgc
      841 gcgggacatc gcgaaccggg tctgcttcct cgacgccggc cggatcctgg agcagggctc
      901 gcccgcgcag atcttctcga acccccagga gccgcggacg cgccagttcc tcaaccggat
      961 catcgaagcc gggcgactgt agccgccccc gggctcagcg gctggcggcg ggtccccggc
     1021 aggcgtccac gaagaacgcg aacaggcgct cgaaggcccg gggcgtggac tcggtgcgtt
     1081 cggggtggca ctggacggcc atccggaacg ggccggcggt cgactcgaag gcctcgacga
     1141 gctcgccgtc cggcccgggc gacacggccg aggcgacgaa ccgcggcgcg aggtcgtccc
     1201 gagcgacgcc ctggtggtgg tagctgttca cggtcaggac cccgcccccg acgttggccg
     1261 ggtacaggat ccgggtgagg cgcgtgcccg ggatcgcgcg cagcgggtgc gtgagggcgt
     1321 caccgtgacc ccagccgtgg cccgcgtggc cgccgatgtc ctggcgcagc cggccgccgg
     1381 cgaacacgtt catcgcctgg agcccgcggc agatcccgaa cacgggcacc gcgcgcgcct
     1441 cggccgccga ccacgctgcg gcctcgaggg cgtcccgctc acgctcgacg ccgtgggcgc
     1501 cgtcgaccgc ctgcccgtac cgcgccgggt cgacgtcagc gcccccgctc agcaggagcc
     1561 cgtccatcgc cgcgaacgcc gtctcgcgct cggcctccgg ggacgtcgcg tccagcggca
     1621 tcgtcaccgc cccgtggcgc gtgagcgcgt cgaggtacag cgcgttcttg cgcgccgcga
     1681 tcccgggctc cgcctgttcg gcggcgacca tcagcgtgac gacgatccga ggcgctccat
     1741 cggcggcttt cgacatgccc ggaggataac ggcgcgccgc ttacggcgag gcgctcggga
     1801 ccggggaccc ggacgggtcc ggcgacgcgt cgaactcctc gtcgtccggc gtgggggtga
     1861 cctcgatgcc cgtctcgccg tcgtcctcgg gcgccacgta ctccttgttc gggatcacct
     1921 ggaggaccca gccgagctcg aggcgatccg gccggtagcg cgcgctctcc ggatcgaggg
     1981 tggggtactt ctcgcggttc cagtacgaga ggctgcgggt cgaggtcttg aaccggcgcg
     2041 cgacccgggt cagggtgtcg ccccgctgca ccttgtagag gcggaaggtc gggcccggcg
     2101 tgggcgtggg cgggcccggc gtcggtgtcg gggtgggggg cgccgtcggg gtagggccgg
     2161 gcgtcggcgg cggcggtgtg gtgggtcgga ccgaggccgg caggcagccg gcgatggcga
     2221 tggccagggc gagggcgccg atgacgaccg gcagggcacc ctgcctgggg tgagcgcgac
     2281 gatggatccg gatcacgtgc agcctccccg tcggccgatt gtgacgcacc cgccggaatc
     2341 ggggtgacgg cgggcccgat ggcccctctc agtccgccgg cacgctcggc gggtcgaccg
     2401 gcgtgggctc cgagagcgtg aactcgctgg cgagcgcgag gccttcggac gccgtcgcga
     2461 ccagcccccg gccgccgcgc ttcgctacga agcacgcgcg gtccgccgcg agcagcacgt
     2521 cttcggcggt ggacccgtcg accgggaacg aggcggtccc gatcgaggcc gcgacgacca
     2581 cgttctccgc gcgccaattg gtgccttcgc cgccgacgcc ggcaacggcg ccacggatcc
     2641 gctccgcaac ggcgagcagc ccgttgggat cgctgagggg caggatcaaa acgaactcgt
     2701 cgccgccgta gcggaacacc gcgtcggtgt cgcgtgcggc cgcccggagg gcctctgcga
     2761 tctcgcgcag gagccggtcg cccgcctggt ggccgagctt gtcgttgacc gccttgaact
     2821 cgtcgaggtc gaccatgacc aggccgaacg tctcgccgcc ggcgacgcta cgggccagcc
     2881 agtccttgaa cgtccggtgg ttcagcaggc cggtcaggtc gtcggtccgg gcgcggaatt
     2941 ccgccgcctg gaaggtctcg gcgttgcgga gcgcgatcga gacctgcgcc gcgaacagct
     3001 tgaccagctc gaactcctgc tcgtcgaacg cggtcgcgtt ccccagccgc tcgagcgtca
     3061 tgacgccgac cgcgccctcg gggccgagca gcgggacgac gatcaggctg ccgtcgatgg
     3121 caccggtcgc gcggaagtgg ttgacgcgtt cgtcggtccg ctcgtcctcg atcaggaccg
     3181 gctcgttgtg ctccacgacc caggtggcga tgccctcctc gccgggctcc cacggctgca
     3241 tgtactcgtc ggagtggatg ccgcgggccg tgaggggaac cagcagcccg gccgaccggt
     3301 ccacgacctc gatcgcgatg ttgtcgcagt ggatcaggcc gcccatgcgg tcggtgatct
     3361 gttcgagcac ggcccgctgg tcgagcgtgg tgaggatcga ctccgtcgtc cgcaggagct
     3421 cgcgctgggc ggcgagctgg cgctcgaggg cgctcgactg ctcgcgcatc atcgccgtcg
     3481 cgtcggcgtt ggtcatcgcc tgggcggcga acgaggcgta gatcacgagc agccgcagat
     3541 cgtcctcggt gaactggcgc aggccgagct tggacagcac gagcacgccg aggcacacgc
     3601 cctcgtgaac catcggggcc agcagcatcg actcgtcgat gtcaggctcc gaccccggga
     3661 tcgtgatcgc ccggggatcg ttcgcggtgt cgtcgaccaa ctgcgggacg cggtacttcg
     3721 cgacccaacc cgtgacgccc tcgccgatct cgacgcgcag gctctccacc gtctcgtcgg
     3781 agtacaccgt gccgtggccc atcatcgcga ccggcatcag gtccttgccc tgcacgcgat
     3841 agacgcgcac gttctggtag tcgatcagct gccgcagctc ggtcgcgatg gcatggccga
     3901 tctcgcgcac gtcggtgagg cccgacagcc gcgacccgag ccgctggatc gactggagct
     3961 gggcggccca ggtcgccatg cgcccgaacg tccgggccga cctgaccgcg atggccgcgt
     4021 cgccggcgag ggcctcggcg ctgtcgaggt cgatctggcg ccagcggtgc ggccggtcgt
     4081 gggcgaggta cagcatcccg tggatctcgt gtccgtcaac gaggggcgcg ctgagcagcg
     4141 tgtcgacccc ctcctggatc gctgcggcgc ggagcgggct gcccgagcgt ggcgtgtccg
     4201 gtccgacgag gatcacggga cggcgggacg gcagctcgcg ctgcccgagg cggtcgatct
     4261 ccagggtgcg tgccgcatcg aggaaggcat cggagaaccc ggtgccgccc ggcgacgaga
     4321 cgcgcccctc cgtgtcgtgg agcacgacgg cgacccggtc ggcgccgaac agcacccgag
     4381 cgtgatcgga gaggtctcgg acgacgtcgc tcacgtcgag ccgggacgtg aggtcggtcg
     4441 cgacccgtcg cagcgcctcg gcgcggcgca gccggccgct ggcctgctcc gaggcattgg
     4501 ccccctggat caggacgccc atcgtccggg cgatggcgcg ggccaggtga tgaccgtcgt
     4561 cccggccgag ctcgaccgcg ccggcgatga ccagcacgcc ccacggatgc tcgccgcccg
     4621 ggatccggac aacgagttcg tcggtgccca tgccgagcag gggctccggc ccgtccaccc
     4681 cggggcgagc gtggatcggc tcgggcgact cgagggcctg cgagaacagg ctgcgtcccg
     4741 gcgggacgtt ccggcccgcc gtgatgcccg ggccctccac gtcggtcgcc cgcgacacga
     4801 ggccgcctgg gcgaacctcc cagatcccta caagcgccgc ccctgtcgcg acgcggatcc
     4861 ggtggcaggc ctggtcgagc gcggcatcga cgccgctcgg gaaggatgcg atctcggcga
     4921 ggtccgccag caggtcgacg ccggccgcgg actcggtgag cgtccgatcg gccatgtcca
     4981 tcgagcggcg cccgatccgc gtcggctggc tcgcgtcggc gatgcccgcg ggcgtgtccg
     5041 acgccgcggc tgtgaggaaa cgctggaggt cgaccagccg gtagcggcga tcgccacgct
     5101 cgttgatccg gtagtagcgc aggcgccccg cctcgctcca cgcgcggatc gtgttcgggt
     5161 gcacacccag cacatgcgct gccttggtga ccgacagcgt cgcatcgccg ggtcgctgga
     5221 cccgggtgcc ggtggctgga atgtgggatt tggcaagcat cgcggtcttg gagggcgctt
     5281 ctcgtggtgt ggcgttgtgc tgggactttc cagcac
//
LOCUS       DBNHXO010000525         7131 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2751727, whole genome shotgun sequence.
ACCESSION   DBNHXO010000525 DBNHXO010000000
VERSION     DBNHXO010000525.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2751727"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="AC162A_10590"
     CDS             <1..585
                     /locus_tag="AC162A_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR5011334.1"
                     /translation="QMDIKVKGIDERVIREGLKQALAARLFILDKMTEVLPAAREEMS
                     DFAPRIITIKINPEKIREIIGKGGSMIRKIQEETQTEINVEDDGTVEIAAVSGENSRK
                     AINWIESLTRDVEVGALYLGKVTRLMNFGAFVEILPGKEGLVRIGELADYHVPSVEDV
                     VSVGDEVMVVVMEIDRQGRVNLSRKAAMQRHLAK"
     gene            complement(655..1395)
                     /locus_tag="AC162A_10595"
     CDS             complement(655..1395)
                     /locus_tag="AC162A_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011335.1"
                     /translation="MDDRSTGPAPLEHPGPAAARRVDTAHGPSPVDDPRALTILTTEH
                     WSLLSARSLVYNEAFSRAGMFLSFLAATLVALGLMSGAMGFSSEFIGISALVLGLDLF
                     IGLATMGRVATATSEDIRFLQGMNRLRHAYHETVPGLDPYFITGHHDDVLGIFANYGA
                     DVQTRTWRSIVHGFTTVVGMLGVINAALSGVVAATIAILIVGESVAAIAIGVGAFLVV
                     LAISVAWSTRAIAATVAGLQPRFPSPGA"
     gene            1683..2678
                     /locus_tag="AC162A_10600"
     CDS             1683..2678
                     /locus_tag="AC162A_10600"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR5011336.1"
                     /translation="MATDYSTSDLTTGAVLPDALTDLSLRQLLSFRAVADEGSFHGAA
                     DALDYTQSAVSQHVMGLEAALGVRLFDRGRGRRTVELTEGGRLLLRHVVTITDRLQAA
                     RADLLAYSAGETGTLRVGVYQSVGTRVLPAIIGRFGEAWPNVEIRLTEANSDQGLFEL
                     LGPGALDLSFGVLPLQEGPFEAVELLHDPFVLVTAAGSPLAGFVGQPSMQLIGEQRLI
                     GFRSCRSTRWVEDHMRARGAEPNFVFRSEDNGTVQAMVGAGLGTAMVPLLAVDATDPA
                     VRIIPTDLPARRIALIWHRERYRSPAALAFVEIACQVAADIQADLAGRVTSAAPA"
     gene            2815..3948
                     /locus_tag="AC162A_10605"
     CDS             2815..3948
                     /locus_tag="AC162A_10605"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR5011337.1"
                     /translation="MPRNAPDSLIVAVVGATGAVGRTMTQVLLEKQFPMARLRLLASE
                     RSAGRRIEVGGTEIEVELATPEAFEGVDIALFSAGGDTSKALAPEAARRGAAVIDNSS
                     AWRMDPAVPLVVSQVNPDDLAWHEGIIANPNCSTMQLLPPLMALRDAVGIERVVVDTY
                     QSVAGTGHKAIVELEDQVRAHAEGRPKVASVYPHPIAFNALPHIDVFLDNGYTKEEWK
                     VVTESRKILHLPELRVSCTAVRVPVFISHSEAVHVETRDPITPDEARALFAGVKGVVV
                     QDDPAANEYPLAENAAGSDDVFVGRVRQDLSIDGNRGLAFWVVSDNLRKGAASNAVEI
                     AEVLVERGWVRPASARDARPAGEQTTASSGEREPVGASGGRTA"
     gene            3945..4694
                     /locus_tag="AC162A_10610"
     CDS             3945..4694
                     /locus_tag="AC162A_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HIR5011338.1"
                     /translation="MTRAERQVALEAIAAEVQACTRCRLHEGRTRAVPGEGDPDTEVV
                     FVGEGPGFNEDREGRPFVGRAGGLLVQLLGSIGWQRDDVFITNVVKCRPPDNRDPQPD
                     EIAACAPYLRRQLEALDPAVVVTLGRYSMGTFMPGARIGQVHGTTAPIDPELGPPDAL
                     AFAMYHPAAALRTPALERESYGDIARVPDVLIRARERRQANATGPAEAAAVALVAPAV
                     APAAPQPLDADASPVFVVDATSDDASQLTLF"
     gene            4714..6381
                     /locus_tag="AC162A_10615"
     CDS             4714..6381
                     /locus_tag="AC162A_10615"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR5011339.1"
                     /translation="MTDPSTPLRIIPLGGVGEIGKNMYCFEYGDDIVVIDCGLMFPDE
                     EMFGIDLVVPDVTYLKENRSKVKAFLITHAHEDHVGGLPYVLPEFPGVPVYASTLARG
                     LLGNKIKEHKLHNNPLLELEPGDELALGPFTAVPFRIGHSIPDAMGIALRTPVGIVVH
                     TGDFKFDHTPVDGKLSDFHILARLGEEGVICLLSDSTRAENPGYTPSERTVGEAFREI
                     MEPLDGRVIVATFASNIARIQQVLDAAAEMDRKVAVIGRSMEQNFKIATDLGYLKYEP
                     SAIVAKDKIKETADSKLVIATTGAQGEPMAGLARMANRDHRFVEIQPGDTVIVSASPI
                     PGNEEYVARTIDNLFKAGANVYYHTIKRAHVSGHASQEELKLMLGLTKPTYFIPMHGE
                     FRMQVQHGRLAIDTGVKPDNVFIIENGTPIEIWPDGSARRGQPVAAGYVYVDGLSVGE
                     VGDVVLRDRRALANDGMFMVVVQVDKQTGRVVGRPEIITRGFVHGNEEDPIIEEAIRR
                     IHQALDQPGDRISEIALLKAQIKDGLSRFLYEQTKRRPMVFPVVVEV"
     gene            6385..>7131
                     /locus_tag="AC162A_10620"
     CDS             6385..>7131
                     /locus_tag="AC162A_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011340.1"
                     /translation="MATRRRTSTSRTSSTRRSQAARAKRGTGLKAPGLSLGMSPAVGR
                     SLVGIVLLVLGAVTLIALMLPGQGALTDWWRNLAVPYFGTGRWLLPFVLLLSGWYLEW
                     GPGREPGAPWGRTLLGIGMAYVGVLGLMQLVEFAGRFTGGRIGRFMVNLLEPLLTTPG
                     AFVILLGLTIAGLLIAFDMPLRALVSPATRAAKAAGTTLQDRTTRAADTVVAAKAADD
                     ARGGRWFAGFVGGLGGDDRVGGPRRPVLERR"
BASE COUNT         1101 a         2500 c         2517 g         1013 t
ORIGIN      
        1 cagatggaca tcaaggtcaa gggcatcgac gagcgggtca tccgcgaagg tctcaagcag
       61 gccctcgccg cccgcctgtt catcctcgac aagatgaccg aggtgctgcc ggcagcccgc
      121 gaggagatgt ccgacttcgc cccgcggatc atcacgatca agatcaaccc cgagaagatc
      181 cgcgagatca tcggcaaggg tgggtcgatg atccgcaaga tccaggaaga gacccagacc
      241 gagatcaacg tcgaggacga cggcaccgtg gagatcgcgg ccgtctccgg cgagaactcg
      301 cgcaaggcga tcaactggat cgagagcctg acccgcgacg tcgaggtcgg cgcgctgtac
      361 ctgggcaagg tcacccggct gatgaacttc ggcgccttcg tcgagatcct gccgggcaag
      421 gaaggcctgg tccggatcgg cgagctggcc gactaccacg tgccgtccgt cgaggacgtc
      481 gtgtcggtgg gcgacgaggt catggtggtg gtcatggaga tcgaccgcca gggccgcgtc
      541 aacctgtcgc gcaaggccgc gatgcagcgc cacctggcga agtaaccctc aacgccaaac
      601 gcccccggag cttcggcttc cgggggcgtt ccgattcccg gggtcagcct gtggtcaggc
      661 gccggggctg gggaacctgg gctggaggcc ggcaacggtc gccgcgatgg cgcgcgtgct
      721 ccacgcgacg ctgatcgcga gaaccacgag gaacgccccg acgccgatgg cgatcgccgc
      781 cacgctctcg cccacgatca ggatcgcgat cgtggcggcg accacgccgc tcagcgcggc
      841 gttgatcacg cccagcatgc cgacgacggt cgtgaagccg tggacgatgg atcgccacgt
      901 cctggtctgc acgtcggcgc cgtagttggc aaagatgccg agcacgtcgt cgtggtgtcc
      961 ggtgatgaag tacggatcga ggccggggac ggtctcgtgg taggcgtgcc gcagccggtt
     1021 catcccctgc aggaaccgga tgtcctcgct ggtggcggtg gcgacccgcc ccatcgtcgc
     1081 gagcccgatg aacaggtcca ggccgagcac gagggccgag atgccgatga actcgctgct
     1141 gaagcccatc gcgcccgaca tcagccccag cgccacgagc gtcgcggcga ggaaactcag
     1201 gaacataccc gcccgcgaga acgcctcgtt gtagacgagc gagcgcgccg acaacaggct
     1261 ccagtgctcc gtggtgagga tcgtcagggc ccgcggatcg tcgacagggg agggaccatg
     1321 ggcggtgtcc acccgccggg ccgccgcagg cccagggtgc tcaagcggcg cgggtccggt
     1381 cgagcggtcg tccatccgcg gcctccagct gcagggtcat gatgagcgca gcatgccgca
     1441 cgcgcgcaac gcccccgaag ccgaccgctc cgggggcgtt gttcgtttcg cgctgtgatc
     1501 gccgggggcg tctaccagac gcggggctgg aaccgcgtgt cggtgtcgcg cgagtcgacg
     1561 ccggccacgc tggcgagcag gccgaggatg acgatgagga gggctgcgac gaggagttcc
     1621 atgtgggcta tcctgcgccg gcctattgat cagagcaatc ggatgtgatc caataagaaa
     1681 tcatggcgac tgattattcg accagcgacc tgaccaccgg tgcggtgctc ccggacgcac
     1741 tcacggacct cagcctccgc cagctcctga gcttccgggc cgtggcggac gagggctcgt
     1801 tccacggcgc cgccgacgct ctcgactaca cccagtccgc cgtcagtcag cacgtgatgg
     1861 ggctcgaggc ggccctgggc gtcaggctgt tcgaccgtgg gcgcggccgc cggacggtgg
     1921 agctcacaga gggcggccgc ctgctgctcc gccacgtcgt gacgatcacc gaccgcctcc
     1981 aggcggcgcg cgcggacctg ctggcctact cggccgggga gaccggcacg ctgcgcgtgg
     2041 gcgtgtacca gagcgtcggc acccgggtgc tgcccgcgat catcggccgg ttcggcgagg
     2101 cgtggccgaa cgtcgagatc cgcctgaccg aggccaacag cgaccagggc ctgttcgagc
     2161 tcctcgggcc cggcgcgctc gatctctcgt tcggcgtgct gccgctccag gaaggcccgt
     2221 tcgaggcggt cgagctcctg cacgacccgt tcgtgctcgt caccgccgcg ggctcgccgc
     2281 tggctggctt cgtcggccag ccgtcgatgc agctcatcgg ggagcagcgg ctgatcggct
     2341 tccggtcgtg ccgcagcaca cgctgggtcg aggaccacat gcgcgcgcgc ggtgccgaac
     2401 cgaactttgt cttcagatcc gaggacaacg ggacggtcca ggcgatggtg ggagcggggc
     2461 tcggaacggc catggtgccg ctcctcgccg tcgacgcgac cgaccccgcg gtcaggatca
     2521 tcccgaccga tcttcccgcc cgtcggatcg cgctcatctg gcaccgcgag cgctaccggt
     2581 cgccggcggc gctcgccttc gtcgagatcg cctgtcaggt cgcggcggac atccaggccg
     2641 acctcgcggg acgcgtgaca agcgcggccc cggcctgacg cgcggcggac gcgtcaacgg
     2701 agtggtgccc gcacgcaccg cgggcgtatc gccggccatg ccgtgcccgg atcggccgga
     2761 acccgctggt atactccgcc ggtgcccgac aacctgtcag caaaaggtcg gaccatgccc
     2821 cgaaacgccc ctgattccct gatcgtcgcg gtcgtcggcg ccaccggcgc cgtgggccgg
     2881 acgatgaccc aggtcctgct cgagaagcag ttcccgatgg cccggctgcg gctgctcgcc
     2941 tcggagcggt ccgccggacg ccggatcgag gtcggcggca cggagatcga ggtcgagctc
     3001 gccactcccg aagccttcga aggcgtcgac atcgccctgt tcagcgccgg cggcgacacg
     3061 tcgaaggcgc ttgctcccga ggccgccagg cgcggcgcgg cggtcatcga caactcgagc
     3121 gcctggcgca tggacccggc ggtcccgctc gtcgtgtccc aggtcaaccc cgacgacctc
     3181 gcctggcatg agggcatcat cgccaacccc aactgctcga ccatgcagct gctgccgccg
     3241 ctgatggccc tgcgggacgc cgtcggcatc gagcgggtgg tcgtcgacac ctaccagtcg
     3301 gtcgcgggca cgggccacaa ggccatcgtg gagctcgagg atcaggtcag ggcgcacgcc
     3361 gagggccgcc cgaaggtcgc cagcgtctat ccgcacccga tcgcgttcaa cgcgctgccg
     3421 cacatcgacg tgttcctgga caacgggtac acgaaggagg agtggaaggt cgtcacggag
     3481 agccgcaaga tcctgcacct gcccgagctc cgggtctcgt gcacggcggt ccgcgtgccg
     3541 gtgttcatca gccactccga ggccgtccac gtcgagaccc gcgacccgat cacgcccgac
     3601 gaggcccgcg cgctgttcgc cggggtcaag ggcgtcgtcg tccaggacga ccccgccgcc
     3661 aacgagtatc cgctggccga gaacgccgcc ggctcggacg acgtgttcgt cgggcgcgtc
     3721 cgccaggacc tctcgatcga cggcaaccgc ggtctcgcgt tctgggtcgt gtcggacaac
     3781 ctgcgcaagg gcgcggcgtc gaacgccgtc gagatcgcgg aggtgctggt cgagcgcggc
     3841 tgggtccgcc cggcgagcgc gcgcgacgca cggccggcgg gagagcagac gacagcgagc
     3901 tctggcgagc gggagccggt cggggcgtcc gggggacgaa ccgcgtgacg cgcgccgagc
     3961 gccaggtggc gctcgaagcc atcgctgccg aagtccaagc gtgcacccgc tgccggttgc
     4021 acgagggccg gacgcgtgcc gtcccgggcg agggcgaccc cgacaccgag gtcgtgttcg
     4081 tgggcgaggg cccgggcttc aacgaggacc gcgagggccg gccgttcgtc ggccgcgcgg
     4141 gcgggctgct ggtccagctg ctgggctcga tcggctggca gcgcgacgac gtgttcatca
     4201 ccaacgtcgt gaagtgccgg ccgccggaca accgcgaccc gcagccggac gagatcgcgg
     4261 cgtgcgcgcc gtacctgcgg cgccagctcg aggcgctcga cccggcagtc gtggtcacgc
     4321 tcggcaggta ctcgatgggc acgttcatgc cgggcgcccg gatcggccag gtccacggca
     4381 cgacggcacc gatcgatccc gagctcgggc caccggacgc gctcgcgttc gcgatgtacc
     4441 acccggcggc cgccctgcgt acgccggcgc tcgagcgcga gagctacggg gacatcgcgc
     4501 gtgtcccgga cgtgctgatc cgggctcgcg agcgccgcca ggcgaacgcg acgggacccg
     4561 ccgaggcggc ggccgtcgcc ttggtcgccc cggctgtcgc gccggccgcg ccccagcccc
     4621 ttgatgccga cgcgtcgccc gtcttcgtcg tggacgccac gtccgacgac gcgtcccagc
     4681 tgaccctgtt ctgaccgggc ggacgacgac cacatgaccg atcccagcac gccgctccgc
     4741 atcatcccgc tcggcggggt gggggagatc ggcaagaaca tgtactgctt cgagtacggc
     4801 gacgacatcg tcgtcatcga ctgcggcctc atgttccccg acgaggagat gttcggcatc
     4861 gacctcgtcg tgcccgacgt gacctacctc aaggagaacc gcagcaaggt caaggcgttc
     4921 ctgatcacgc acgcgcacga ggaccacgtc ggcggcctgc cgtacgtgct gcccgagttc
     4981 ccgggcgtgc ccgtgtatgc cagcacgctc gcccgcggcc tgctgggcaa caagatcaag
     5041 gagcacaagc tccacaacaa cccgctgctg gagctcgagc cgggcgacga gctcgcgctg
     5101 gggccgttca cggccgtgcc gttccggatc ggccactcga tccccgacgc gatgggcatc
     5161 gcgctgcgga cgccggtggg catcgtcgtc cacacgggtg acttcaagtt cgaccacacc
     5221 cccgtcgacg gcaagctgtc ggacttccac atcctcgccc gtctcggcga ggagggcgtc
     5281 atctgcctcc tgtccgactc gacccgcgcg gagaacccgg gctacacgcc gtcggagcgc
     5341 acggtggggg aggccttccg cgagatcatg gagccgctcg acggccgggt gatcgtcgcc
     5401 accttcgcca gcaacatcgc ccggatccag caggtgctcg acgcggccgc cgagatggac
     5461 cgcaaggtcg cggtgatcgg ccggtcgatg gagcagaact tcaagatcgc caccgatctc
     5521 ggctacctca agtacgagcc gtccgcgatc gtcgccaagg acaagatcaa ggagacggcc
     5581 gacagcaagc tcgtgatcgc gacgaccggt gcccagggcg agccgatggc ggggctcgcc
     5641 cggatggcca accgcgacca ccggttcgtc gagatccagc ccggcgacac cgtgatcgtg
     5701 tcggccagcc cgatccccgg caacgaggaa tacgtcgccc gcaccattga caacctgttc
     5761 aaggcgggtg cgaacgtgta ctaccacacg atcaaacgcg cccacgtctc aggccacgcg
     5821 agccaggagg agctcaagct gatgctcggg ctcaccaagc cgacctactt catcccgatg
     5881 cacggcgagt tccggatgca ggtccagcac ggacgcctcg cgatcgacac cggcgtcaag
     5941 cccgacaacg tgttcatcat cgagaacggc acgccgatcg agatctggcc ggacggctcg
     6001 gcgcgccgcg gccagccggt cgcggcgggc tacgtctacg tcgacggtct gtccgtaggc
     6061 gaggtcggcg acgtcgtgct gcgcgaccgc cgggcgctcg ccaacgacgg gatgttcatg
     6121 gtcgtggtgc aggtcgacaa gcagaccggc cgggtcgtcg gccggccgga gatcatcacc
     6181 cgcgggttcg tccacggcaa cgaggaggac ccgatcatcg aggaggcgat ccgccggatc
     6241 caccaggcgc tcgatcagcc gggtgaccgg atcagcgaga tcgcgctgct caaggcgcag
     6301 atcaaggacg gcctctcgcg cttcctgtac gagcagacga agcggcgccc gatggtcttc
     6361 ccggtcgtgg tcgaggtcta gggggtggcc acgcggcgac gcaccagcac ctcgcgcacg
     6421 tcctccaccc ggcgctcgca ggccgcgcgc gccaagcgcg ggaccgggct gaaggcgccc
     6481 gggctgtcgc tcggcatgtc gccggcggtc gggcgctcgc tggtgggcat cgtgctgctc
     6541 gtgcttggtg cggtgacgct gatcgcgctg atgctcccgg gccagggcgc cttgaccgac
     6601 tggtggcgca acctcgcggt gccgtacttc gggacgggcc gctggctgct gccgttcgtg
     6661 ctgttgctgt cgggctggta cctcgagtgg ggcccgggcc gcgagcccgg cgcgccgtgg
     6721 ggccggacgc tgctgggcat cggcatggcc tacgtgggcg tgctcgggct gatgcagctc
     6781 gtcgagttcg ccggccggtt cacgggcggt cgcatcgggc gcttcatggt caacctcctc
     6841 gagccgctgc tgacgacgcc tggggcgttc gtgatcctgc tcggcctgac gatcgcgggg
     6901 ctgctgatcg ccttcgacat gccgttgcgg gcgctcgtct cgccggcgac gcgcgccgcc
     6961 aaggccgccg ggacgacgct ccaggaccgg acgacgcggg ccgccgatac ggtcgtcgcc
     7021 gcaaaggccg ccgacgacgc ccgcggcggc cgctggttcg cgggcttcgt cggcggcctt
     7081 ggcggcgacg accgtgtcgg cggcccgcgt cgtccggtcc tggagcgtcg t
//
LOCUS       DBNHXO010000526         3945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_769252, whole genome shotgun sequence.
ACCESSION   DBNHXO010000526 DBNHXO010000000
VERSION     DBNHXO010000526.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_769252"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="AC162A_10625"
     CDS             complement(<1..859)
                     /locus_tag="AC162A_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR5011341.1"
                     /translation="MSVDASSPSQALLDRSRRLGANPRNTNYAGGNTSAKGTAIDPVT
                     GGLVELLWVKGSGGDLGTLTDAGLAVLRLDRLRALMDVYPGIEREDEMVAAFDYCLHG
                     RGGAAPSIDTAMHGLVDAAHVDHLHPDSGIALATAADGEALTREAFGDRVAWVPWRRP
                     GFQLGLDIAAIRAANPAAIGCVLGGHGITAWADSSEACEANSLDIIGTAERFISDRGR
                     PEPFGPLLPGYEALPQAERRARAAALLPIVRGLASVDRPQVGHFNDADVVLDFLARAE
                     HPRLAALGTS"
     gene            complement(856..2022)
                     /gene="rhaI"
                     /locus_tag="AC162A_10630"
     CDS             complement(856..2022)
                     /gene="rhaI"
                     /locus_tag="AC162A_10630"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017607910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019318 - hexose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HIR5011342.1"
                     /translation="MTTESTILDALAGHRIELPSWAFGNSGTRFKVFAQPGVPRDPYE
                     KVADAAEVHRYTGVAPTVALHIPWDKVADYADLARHAQDCGVALGTINSNTFQDDDYM
                     LGSVCHPDPRVRQKAIDHLLECVDIMDATGSRDLKLWFADGTNYPGQDDIRARQDRLA
                     EALETVYGRLGDAQRMVLEYKLFEPSFYTTDVPDWGTALVHCLRLGDQALVVVDTGHH
                     APGTNIEFIVASLLRAGKLGAFDFNSRFYADDDLMVGSADPFQLFRIMHEVVKADALR
                     PEAGVAFMLDQCHNIEPKIPGQIRSVMNVQEATAKALLVDRTALETAQRTGDVLGANA
                     VLMDAYNTDVRPLLGRLRERMGLDPDPMAAYARSGYAARIVAERVGGNQAGWGA"
     gene            2324..3145
                     /locus_tag="AC162A_10635"
     CDS             2324..3145
                     /locus_tag="AC162A_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012669.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR5011343.1"
                     /translation="MTVPTGAASGDVFARERQDDIARLVTEQGRVRVRDLATRFGVST
                     VTIRKDLDVLESEGRLMRTHGGALTPIRDRPEPAFDLRERLQQDEKARIGAAAAALVH
                     DGDSIVMDASTTGLAVARRIRQQGGWSQLTVITNGLRIATELAGTQGITVLMTGGRVR
                     WQAMSVVGHLGDGLFDRINVQTAFVGAAGFALEPGLSDATEEEAQIKRSMVAAARRVI
                     AIVDHTKWQRVTFATFCRTGEIDAVLTDDQAPTDMVRDLRARGIEVTMIPPVSPA"
     gene            3199..>3945
                     /locus_tag="AC162A_10640"
     CDS             3199..>3945
                     /locus_tag="AC162A_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5011344.1"
                     /translation="MSKSFAATQALTDVSLDLRAGEVHALVGENGAGKSTLVKILAGV
                     HQPDTGTIRVDGEPRVIAGPAQARALGIAVVHQEPRLFPDLTVAENVFIGHAPSGPLR
                     TVDWGGMRREARKLFEELDVKFDVQAQVRGMSMADQQLIEIAKALSVEAGVLILDEPT
                     ASLSAHEVDRLFTIVRGLRDRGVAVLFVSHRLGEVFDLCDTATVLRDGRHVITTSTKE
                     LTTADLIRHMVGRTVTLFPKVETAVGDVLLE"
BASE COUNT          565 a         1398 c         1406 g          576 t
ORIGIN      
        1 acgacgtgcc gagcgcggcg aggcgcgggt gctcggcccg ggccaggaag tcgagcacga
       61 cgtccgcgtc gttgaagtga ccgacctgcg ggcgatcgac cgaggccagg ccacgcacga
      121 tcgggagtag cgcggccgct cgcgcccggc gctcggcctg cggcagggcc tcgtaccccg
      181 gcagcagcgg accgaacggc tccggtcgtc cgcggtccga gatgaaccgc tcggccgtcc
      241 cgatgatgtc cagcgagttc gcttcgcacg cctctgacga gtcggcccag gcggtgatcc
      301 cgtgcccccc gaggacacag ccgatggccg ccgggttcgc ggcgcggatg gcggcgatgt
      361 cgaggccgag ctggaacccc ggccgacgcc acggcaccca tgccacccga tcgccgaacg
      421 cctcgcgcgt cagcgcctcg ccatcggccg cggtggccag cgcgatcccc gagtcgggat
      481 ggaggtggtc gacgtgggcc gcgtccacca ggccatgcat ggcggtgtcg atcgacggcg
      541 cggcgccgcc ccggccgtgg aggcagtagt cgaacgccgc gaccatctcg tcctcgcgct
      601 cgatgcccgg gtagacgtcc atcagcgcgc gcagccggtc gagccgcagc accgcgagcc
      661 cggcgtcggt cagcgtgccc aggtcaccgc ccgagccctt gacccacagc agctcgacga
      721 gcccgccggt caccggatcg atcgccgtcc ccttggccga ggtgttgccg cccgcgtagt
      781 tcgtgttgcg cggattcgcg ccgagacggc gagaccggtc gagcagcgcc tggctgggcg
      841 aggacgcgtc gacgctcatg caccccaccc ggcctggttg cccccgaccc gttcggcgac
      901 gatccgcgcc gcatacccgg accgcgcgta ggcggccatc gggtctgggt cgaggcccat
      961 ccgctcccgc agccgcccga gcagcggccg gacgtcggtg ttgtaggcgt ccatcaggac
     1021 ggcattcgcg cccaggacat caccggttcg ctgcgccgtc tcgagcgccg ttcggtcgac
     1081 gagcagcgcc ttcgccgtcg cttcctgcac gttcatcacc gatcggatct ggcccgggat
     1141 cttgggctcg atgttgtgac actggtcgag catgaacgcc acgcccgcct cgggtcgcag
     1201 cgcgtcagcc ttcacgacct cgtgcatgat ccggaacagc tggaagggat cggcggatcc
     1261 gaccatcagg tcgtcgtccg cgtagaaccg cgagttgaag tcgaacgccc cgagcttccc
     1321 cgcccgcagc agcgaggcga cgatgaactc gatgttggtc cccggcgcgt ggtggccggt
     1381 gtcgacgacg acgagcgcct ggtcacccag ccgcaggcaa tgaacgagcg ccgtgcccca
     1441 gtcgggcacg tcggtcgtgt agaacgacgg ctcgaacagc ttgtactcga gcaccatccg
     1501 ctgcgcgtcg ccgaggcgcc cgtagaccgt ctcgagggcc tcagcgaggc gatcctggcg
     1561 ggcgcggatg tcgtcctggc ccgggtagtt ggtgccgtcg gcgaaccaca gcttgaggtc
     1621 ccgcgagccg gtggcgtcca tgatgtcgac gcactcgagc aggtggtcga tcgccttctg
     1681 ccggacccgc gggtccggat ggcagacgct gccgagcatg tagtcgtcgt cctggaacgt
     1741 gttcgagttg atcgtcccga gggcgacgcc gcagtcctgc gcgtggcgcg cgaggtccgc
     1801 atagtcggcg accttgtccc acgggatatg caacgcgacc gtcggcgcga cgccggtgta
     1861 ccggtggacc tcggccgcgt cggcgacctt ctcgtacggg tcgcgcggca cgcccggctg
     1921 cgcgaacacc ttgaaccgcg tcccggagtt gccgaacgcc caggacggca gctcgatccg
     1981 gtgcccggcc agtgcgtcga ggatggtgga ctcggtcgtc acgtgcgcct ccgtcgcccg
     2041 tcgccggcga tccgtgcgcg gactcaccgg gacgatggta ggggctcgcg gccggggaat
     2101 tcgaccggac gggcgacgat gtcggaggag tagacatcgt cgcccggtgc ccggtcgagc
     2161 tcctgatcca gccgatcaaa agctcggtcc tctcagcctg ccacaggttt cgtttcattt
     2221 caatctagtt tcttgacatt gcggatcgct tcgggccaag atgcgccgac acccgcgtga
     2281 cacctctccg tcccgtgccc gggacccgtg tggaggccgc ttcttgaccg tcccgaccgg
     2341 cgccgcgtcc ggcgacgtct tcgcccgcga acgccaggac gacatcgccc gcctcgtgac
     2401 cgagcagggc cgcgtgcggg tccgggacct cgccacccgg ttcggcgtgt ccacggtgac
     2461 gatccgcaag gacctcgacg tcctcgagag cgaggggcgc ctgatgcgca cgcacggcgg
     2521 ggcgctgacg ccgatccgcg accgcccgga gcccgcgttc gacctccgcg agcgcctcca
     2581 gcaggacgag aaggcgcgca tcggagccgc cgccgccgcg ctcgtgcacg acggcgactc
     2641 gatcgtcatg gatgccagca ccactgggct cgcggtcgcg cgccgcatcc gccagcaggg
     2701 cggctggagc cagctgacgg tgatcaccaa cgggttgcgc atcgcgaccg agctcgcggg
     2761 gacgcagggg atcacggtcc tgatgaccgg gggccgggtg cgctggcagg cgatgtcggt
     2821 cgtgggccac ctgggcgacg ggctattcga ccggatcaac gtccagaccg ccttcgtcgg
     2881 cgccgcgggg ttcgcgctcg agcccggcct ctcggacgcg accgaggaag aggcccagat
     2941 caagcggtcg atggtcgccg ccgcccggcg ggtcatcgcg atcgtcgacc acaccaagtg
     3001 gcagcgcgtg acgttcgcga ccttctgccg gaccggggag atcgacgccg tgctgaccga
     3061 cgaccaggcg ccgaccgaca tggtccgcga cctgcgcgca cggggcatcg aggtgacgat
     3121 gatcccgccg gtttcgccgg catgacggcg atggcggacg ccgtggcggg ggcggcggtt
     3181 cgcgcttcgc ttgccggcgt gtcgaagtcg ttcgccgcga cccaggccct gacggacgtg
     3241 tcgctggacc tgcgcgccgg cgaggtccat gcgctcgttg gggagaacgg cgccgggaag
     3301 agcacgctcg tcaagatcct cgccggcgtc caccagcccg acaccgggac gatccgcgtc
     3361 gatggcgagc cgcgggtgat cgcaggcccg gcccaggcgc gggcgctcgg gatcgcggtc
     3421 gtccaccagg agccgcggct gttccccgat ctcaccgtcg ccgagaacgt gttcatcggc
     3481 cacgcgccgt cgggtcccct gcgcacggtc gactggggag ggatgcgccg cgaggcgcgg
     3541 aagctgttcg aggagctcga cgtcaagttc gacgtccagg cgcaggtccg cggcatgtcg
     3601 atggcggacc agcagctgat cgagatcgcc aaggcgctgt cggtcgaggc cggggtcctg
     3661 atcctcgacg agccgaccgc ctcgctgtcg gcgcacgagg tcgaccgcct gttcacgatc
     3721 gtccgcggcc tgcgggaccg cggcgtggcc gtgctgttcg tgagccatcg gctgggcgag
     3781 gtgttcgacc tgtgcgacac cgcgaccgtc ctgcgcgacg gccgtcacgt catcacgacc
     3841 tcaacgaagg agctgacgac ggccgacctg atccggcaca tggtcggccg gaccgtcacg
     3901 ctgttcccca aggtcgagac ggcggtcggc gatgtgctgc tcgag
//
LOCUS       DBNHXO010000527         1709 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4539740, whole genome shotgun sequence.
ACCESSION   DBNHXO010000527 DBNHXO010000000
VERSION     DBNHXO010000527.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4539740"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..853
                     /locus_tag="AC162A_10645"
     CDS             116..853
                     /locus_tag="AC162A_10645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5011345.1"
                     /translation="MDERAFYGPEQSGIHHEAFGRLAGRAADLLTAELAAAGLAAGTI
                     VDLGCGSGILARRMTDAGFDVIGVDLSAAMLEIARREAPGARFVQGSLLDAELAPGSV
                     AVTATGEALNYAVDARAGDDAFAALAARVAAALVPGGVLLFDVSVHGRSGPDRLRVQF
                     HDRPGWSVGVRETEDDVSVTRAIATFRQLDDGRYERTDERHVLHLYDPDSLRSALEGV
                     GFDVDLRAGYEPGESMMGWVVVLARRR"
     gene            complement(868..1368)
                     /locus_tag="AC162A_10650"
     CDS             complement(868..1368)
                     /locus_tag="AC162A_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011346.1"
                     /translation="MTTPSPRRVVVHSPRPADEALEILANLLTGEPRPIRSRASPGIV
                     LLEGAVDARGVVFTVIPRTRAAGPGQPTESRTLEFVGAIDATSDGSMLTGSISAPPTA
                     LGLPAAAIAALVALFLIWNGIPLPLVAVGVVAWIFLTVIVVAGIGEQRLAGADSIGRL
                     LEDALA"
     gene            complement(1383..>1709)
                     /locus_tag="AC162A_10655"
     CDS             complement(1383..>1709)
                     /locus_tag="AC162A_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR5011347.1"
                     /translation="LVGPARTRDVILTGRRLSAPEAEAWGVVARVVEAGRARDEAVAL
                     AASIAEGAPLGIRQAKAAIRGAHRALADGLEAENELYEIVLVSDDRREGFRAFTERRK
                     PRFTGR"
BASE COUNT          222 a          626 c          620 g          241 t
ORIGIN      
        1 cggttggcgt cgaacgagga gccgcgaggc gtgtcggcga ggacggtcat cggccccatt
       61 agcatcgggc atccgccgcg tgtcaagcgg gccggcccgc tagcatcggc tggcgatgga
      121 tgagcgcgcg ttctacggcc ccgagcagtc cggtatccac cacgaggcgt tcgggcggct
      181 cgccggccgg gcggcagacc tgctgacggc ggagcttgcc gccgcgggcc tggccgcggg
      241 cacgatcgtg gacctcgggt gcgggtcggg catcctcgcg cgccgcatga ccgacgcggg
      301 cttcgacgtg atcggggtgg acctgtcggc cgcgatgctc gagatcgcgc ggcgcgaggc
      361 gcccggcgcc cgattcgtgc agggcagcct actcgacgcc gagctggcgc cagggtcggt
      421 cgccgtgacc gcgaccggcg aggcgctcaa ctacgcggtc gatgctcggg cgggcgacga
      481 tgccttcgcg gccctcgccg ctcgcgtcgc ggccgccctc gtccccggtg gagtgctgtt
      541 gttcgacgtt tcggtgcacg gcaggtccgg gccggaccgc ctgcgcgtcc agttccacga
      601 ccgtccgggc tggtcggtcg gggttcggga gaccgaggac gacgtgtccg tcacccgtgc
      661 catcgcgacg ttccgccagc tcgatgacgg tcgctatgaa cgcaccgatg aacgccacgt
      721 gcttcacctc tacgacccgg actcgctccg gtccgcgctc gagggtgtgg gtttcgacgt
      781 cgacctgcgg gcgggctacg agccgggaga atcgatgatg ggctgggtcg tcgtcctcgc
      841 acgccgccgc tgaccgcggt cgccggttca ggcgagggca tcctcgagca ggcgcccgat
      901 cgaatcggcg cccgcgagcc gctgctcgcc gatcccggcc acgacgatga cggtcaggaa
      961 gatccaggcc acgacgccga ccgcgacgag cggcagcggg atgccgttcc agatgaggaa
     1021 caaggcgacg agggcggcga tcgccgcggc cggcaggccc agcgccgtcg gcggggcgct
     1081 gatcgacccg gtcagcatgc tgccgtccga tgtcgcgtcg atcgcaccca cgaactcgag
     1141 cgtcctcgac tccgtcggtt gtcctgggcc ggcggcgcgc gtcctcggga tgaccgtgaa
     1201 tacaacccca cgcgcgtcga ccgctccttc gaggagcacg atgcccggcg acgcgcgcga
     1261 tcggatgggg cggggctcgc cggtcagcag gttcgcgagg atctccagcg cctcatcggc
     1321 cggccgcggc gagtggacca cgacccgccg gggcgacggc gtggtcacgt cgtgccacag
     1381 gctcaccgac ccgtgaagcg cggcttgcgc ctctcggtga acgcccggaa gccctcccgc
     1441 cggtcgtcgc tgaccagcac gatctcgtag agttcgttct cggcttcgag tccatcggcc
     1501 agcgcccgat gggcaccgcg gatcgccgcc ttcgcctggc ggatccccag cggcgctccc
     1561 tcggcgatcg aggccgccag ggccactgcc tcgtcacgcg cccgccccgc ctcgacgacc
     1621 cgtgcgacca cgccccacgc ctcggcctcc ggcgccgaca gccggcggcc ggtcaggatc
     1681 acgtcccggg ttctcgccgg cccgacgag
//
LOCUS       DBNHXO010000528         9039 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5335264, whole genome shotgun sequence.
ACCESSION   DBNHXO010000528 DBNHXO010000000
VERSION     DBNHXO010000528.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 9039)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9039)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9039
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5335264"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="AC162A_10660"
     CDS             complement(<1..604)
                     /locus_tag="AC162A_10660"
                     /inference="COORDINATES: protein motif:HMM:NF016593.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR5011348.1"
                     /translation="MTDPLSLTEAKRLAGTADTILLRATRTADLETPIGAFLRLDDGK
                     TPAYLLESVEGGERLGRYSFLGVGPRRLLEVGDGVARTTTRPVSVDSYSPDLPVTESQ
                     VPDPLAALRAFVPRRRVLPTEGMPRFTGGAVGALSYDAVTTFEPSVPRPEKDPVGVPL
                     AAFIETDLVLVFDHLSHQLSAIASLHTDAPDFEGRYRIAER"
     gene            complement(977..2203)
                     /locus_tag="AC162A_10665"
     CDS             complement(977..2203)
                     /locus_tag="AC162A_10665"
                     /inference="COORDINATES: protein motif:HMM:NF037144.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5011349.1"
                     /translation="MAAEHTRRLRAASVIAAGAIGASVLWAAPASAGSLGIGSAAMLV
                     PPVFPHLPMGDAWSVSHDQKLRVDEPGVLANDGPSHSGEMRAEVLTEPLFGELNLNHD
                     GGFDYEPNEGFVGIDTFIYTADDDTGPMPATVVITVTNERPDGHDDEYATDEGKELSV
                     DKPGPLANDDDGDGDELVAKQITNPAHGSLSMDSRGDFEYHPDDGFSGKDSFNYRPFD
                     GVEFGPVTKVRITVRPKPASTPVPTPSPTATPEPTPRATPEPTPKPTPEPTPRPTPEP
                     TPTPSPTREPTPRPTREPTPTESPSPTEDAESATPAGVVIGPNGGSGPPPGDTLVIQR
                     APTGGTSSDSVTLDAGLAAAFDGFTWQVPMLVLTVPGLLVVLAVGLQILAGAVWIPVI
                     RRRLGGDGLTPPDRRP"
     gene            complement(2176..3984)
                     /locus_tag="AC162A_10670"
     CDS             complement(2176..3984)
                     /locus_tag="AC162A_10670"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphohydrolase"
                     /protein_id="HIR5011350.1"
                     /translation="MRRPRPVLLLIVYGVFLVIVGVTASAQVMLASLHVSTSALNQAV
                     TADTQIVRGFVDDLLVPADLEAPGPDVRRLATLQAGLQAFVAPRGILRAEIRRPDGTI
                     VASDAPIAGFRAAGSPDWATAIAGQTAVGITAAAVSEAGPGELGAETMVREYLPLLRD
                     GQVAGVVAVWRDAAPIMAAIDAARRDVVLVTVSAAVAAAIVLYLVFRSAQARITRQTV
                     ALLEATRRDPFTGMLNHGELVDLVEAAIERLRGTGEGLEIALLDIDNFRILNDTWGHA
                     AGDDAISAVGDRLRPLAASSLAVGRYGPDEFLVAVEGPAVGTLLRTMEQVQRDLAEVR
                     LRFGDSQDLPVTISGAIARFPDDGDSVTELFANAAVTLREAKAGGGGAVLVTAFDVDA
                     TVLSGSFDVFQGLILAVDAKDRYTKRHSEDVARYAMFIATKSGLGAEELDTLRLAGLL
                     HDVGKVGIPDQILRKPGKLTDAEFDIVKQHVALGDAILRDLPDLDTIRAGVRHHHERW
                     DGRGYLHALAGEDIPLFARILAVADTFSAMTTSRPYRKALDLREARMRLEDAAGSQLD
                     ERLVKAFLDGIESDANPPLPGDDVAARLWLPNTRVA"
     gene            complement(4194..4826)
                     /locus_tag="AC162A_10675"
     CDS             complement(4194..4826)
                     /locus_tag="AC162A_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011351.1"
                     /translation="MDGGRSFGEAREPTQFAAFDGLVETCRRTRAALVAVVHERSGDG
                     EMVRAVIADDGLAHVDAVRAGLTALAKAGQFEAAELRDLVRREGILEVPRSGPDAVAA
                     AAEASVNRPPFRPADTGQILAAGHARIVFSVIPTLPSEAVTWPRVHRTYADIPTPRSE
                     AELFARVDELARVLWRVAEGRHRRDEQMRRALAFYEAGSRLSLRGGFSAA"
     gene            complement(4829..5284)
                     /locus_tag="AC162A_10680"
     CDS             complement(4829..5284)
                     /locus_tag="AC162A_10680"
                     /inference="COORDINATES: protein motif:HMM:NF014229.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HIR5011352.1"
                     /translation="MGEHLEVCEACVAEGTRWVHLRQCLVCGQTLCCDNSPRRHMRGH
                     WQGVGHAVMKGVSPGDDWIWCFADDAPIREAGDGWETYDAFAEAGVPVMAAHIAAGAG
                     MPRPDYMTDDEFPLGDWLGYVRDLHHDKELDPADEQAIEAIPGWSWGGG"
     gene            5522..7531
                     /locus_tag="AC162A_10685"
     CDS             5522..7531
                     /locus_tag="AC162A_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HIR5011353.1"
                     /translation="MRSHARGQVRLLLLAMVVAAVAVPVAPAERTNAATGVSGVFIDS
                     EPGESVGEGRQVVYDAGNSSFGFMSGSDESLWVELGTTDSFGHWVMMFAPAPGGTLVV
                     GTYDNAVGGSFPMLSVSHGGLIGCSSFGGWFTINEYAHDPGGVITALSLTFRQVCQPG
                     GKSLYGEVRYQAANGFKAATLSPTSRTFDSALVGDTGDQQTFTLTSAGQLDLEVGSVS
                     MAGEAAADYEVAGDCAPGTLPAGSSCTMTVTFAPETDGTRQAVLSIADDTVRGERSSA
                     LSGVGRLFIWGSPGAAGGNYAWNWGHALARTSPASGGPYLHALYTTNRVGGKWIKDSG
                     PYAGVTYIRSSDNGTSWGSPRRINPSSQHGDREAVAASGNYVYVTWVRVKKWVHFKGS
                     DPRALYIRVSPDNGATWKPIKRLTSASGRVDVSSVAASGSSVYVAYTNSSTGDVRLAV
                     SRNHGRTWHTTKVGTTKLKAAQGRVGLAVVAASGSNVGVAWRSGLQQVKGRVSTNSGK
                     SWKAAVELGGNSDSPTVAAAGSRVVFAWMQNLDLRVRTWNGAWSPEIRVDAGGDLSGQ
                     LPINVTVALNGSSRIGLAWAGCLTGCDDTYNANSRVGLFWRTSRDGGATWEPRDTLAT
                     GDEASSRHINDTPSIDWSVTDAPVVLFNGWSPGTNDYRLYLMQPS"
     gene            7746..8102
                     /locus_tag="AC162A_10690"
     CDS             7746..8102
                     /locus_tag="AC162A_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5011354.1"
                     /translation="MLKAIATAFDEHDLDGIMVHFADDAIFEAPRGADPWGTRFVGSD
                     QVRQAFAARFSGIPDIRYQQDEHFADGDRGASEWTLSGTTTDGQRIEVRGCDLWTFRD
                     GTVVKKDSFWKIRTGP"
     gene            complement(8106..8193)
                     /locus_tag="AC162A_10695"
     tRNA            complement(8106..8193)
                     /locus_tag="AC162A_10695"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8157..8159),aa:Ser,seq:tga)
     gene            complement(8226..8675)
                     /locus_tag="AC162A_10700"
     CDS             complement(8226..8675)
                     /locus_tag="AC162A_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HIR5011355.1"
                     /translation="MEHAQANLNRTAFDATVHCLTGCAIGEVLGLVIASALGLPDLPS
                     VLLAIVLAFTFGYALTMRPLIASGIGLRRAGRIALAADTVSITVMEIMDTLIVLAIPG
                     AMAAGLLDGLFWGSLALALFVAFLVALPVNRWLISRGLGHAVVSGHH"
     gene            8801..>9039
                     /locus_tag="AC162A_10705"
     CDS             8801..>9039
                     /locus_tag="AC162A_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5011356.1"
                     /translation="MFRRPRNGTNGSGGQEVPPPGHQHLIDLRSVVKDYVTDAGPFRA
                     IDGVDLQVDKGEFIAVVGRSGCGKSTLMNMITGIDR"
BASE COUNT         1322 a         3079 c         3284 g         1354 t
ORIGIN      
        1 cccgctcggc gatccggtag cggccttcga agtccggcgc gtcggtgtgg agcgacgcga
       61 tcgccgagag ctggtggctg aggtggtcga agaccaggac gaggtcggtc tcgatgaacg
      121 ccgccagcgg cacgccgacc gggtccttct ccgggcgtgg gaccgacggc tcgaacgtcg
      181 tgacggcgtc gtacgagagg gccccgacgg cgccgccggt gaacctgggc atcccctcgg
      241 tcgggagcac gcgacggcgc gggacgaacg cgcgcagcgc ggccaggggg tccgggacct
      301 ggctctcggt gaccggcagg tccggcgagt agctgtccac gctcacggga cgggtcgtgg
      361 tgcgggcgac gccgtcaccc acctcgagaa gccggcgcgg gccgacgccc aggaagctgt
      421 accgcccgag ccgctccccg ccctcgaccg actcgagcag gtaggccggg gtcttcccgt
      481 cgtcgagccg caggaacgcg ccgatcggcg tctcgaggtc ggccgtccgc gtggcgcgca
      541 gcaggatcgt gtcggccgtg ccggcgaggc gcttggcttc ggtcaggctc agcgggtcgg
      601 tcatcgcggt ccgtccttcg agttggctca gcggctcggg ggccggcggc gcccgagaat
      661 caagaaacga ccttcgtact tcatcgggac gaaaggtcgt gccttccgcg gtgccaccct
      721 cattcggcgg tgtgccgctc tctgaccgac ggagccggtt gcccggttcg atcggcgctg
      781 ccctgtatcg ctggcgccct gcgccggagc ctacttgtcg tccgcggcgc tgcgccgcct
      841 cgcgttcggt ccggtggctc ccgggtccat tcgccgccgc cgccatcccg gcttccacca
      901 gccgccgggt ctctggaatg gcgctccacg ggtactcgtc ccgatcaaag cccgtattcg
      961 cgatgtggcg cggagtctag gggcgacgat ccgggggcgt caagccgtcc ccgccgagcc
     1021 ggcgccggat gacagggatc cagacggcac cggccaggat ctggagtccg acggccagga
     1081 cgacgagcag accgggcacg gtcagcacga gcatcggcac ctgccaggtg aacccgtcga
     1141 aggcggccgc gaggccagcg tcgagcgtga ccgagtcgct cgacgtcccg ccggtcggcg
     1201 cacgctggat cacgagcgtg tcccctggcg gggggccgct cccgccattg ggtccgatca
     1261 cgacgccagc cggtgtcgcg gactcggcat cctcggtcgg tgacggcgac tccgtcgggg
     1321 tcggctcacg cgtcgggcgg ggcgtgggtt cgcgtgtggg cgagggtgtg ggagtgggct
     1381 cgggagtcgg ccttggcgtt ggctcgggcg tgggcttcgg ggtcggctcg ggcgtggccc
     1441 ttggggttgg ctccggcgtc gccgtcggcg acggggtcgg gacgggcgtt gatgcgggct
     1501 tcggtcggac cgtgatcctc accttggtca cggggccgaa ctcgacgccg tcgaacggcc
     1561 ggtagttgaa cgagtccttc ccgctgaagc catcgtcggg gtgatactcg aagtcgccgc
     1621 gcgagtccat cgagagactg ccgtgggcgg ggttcgttat ctgcttcgca acgagctcgt
     1681 cgccgtcacc gtcgtcgtcg ttggccagcg ggcctggctt gtcgaccgag agctcctttc
     1741 cctcatcggt cgcgtactcg tcgtcgtgcc cgtcgggccg ctcgttcgtc accgtgatca
     1801 cgaccgtggc gggcatcgga ccggtgtcgt cgtcggccgt atagatgaac gtatcgatcc
     1861 cgacgaaccc ctcgttcggc tcgtagtcga acccaccgtc gtggttgagg ttgagctcgc
     1921 cgaacagcgg ctccgttagg acctccgcgc gcatctcgcc gctgtggctg ggaccatcgt
     1981 tggcgagcac gccgggttca tccacccgca gcttctgatc gtggctgacc gaccaggcgt
     2041 cgcccatcgg gagatgcggg aacaccggcg gcacgagcat cgcggcgctg ccgattccga
     2101 gcgatcctgc cgacgccggc gcggcccaca gcaccgacgc gccgatggcg ccggccgcga
     2161 tgaccgaggc cgccctcagg cgacgcgtgt gttcggcagc caaagtcgtg cggcgacgtc
     2221 atcgccggga agcggggggt tggcgtcgct ctcgatgccg tcgaggaatg ctttcaccag
     2281 ccgctcgtcg agctgcgagc cggccgcgtc ctccagccgc atccgcgcct cccgcaggtc
     2341 cagcgccttg cggtagggcc ggctggtcgt catcgccgag aacgtgtcgg cgacggcgag
     2401 gatccgggcg aacagcggga tgtcctcacc cgcgagggcg tggaggtagc cgcgtccgtc
     2461 ccagcgctcg tgatggtggc ggaccccggc gcggatcgtg tcaaggtcgg ggaggtcgcg
     2521 caggatcgcg tcgcccagcg ccacgtgctg cttgacgatg tcgaactcgg catcggtgag
     2581 cttgcccggc ttgcgcagga tctggtccgg gatgcccacc ttgccgacgt cgtggaggag
     2641 ccccgccaga cgaagcgtgt cgagctcctc cgccccgagc ccggacttcg tcgcgatgaa
     2701 catcgcatac cgggcgacgt cctccgagtg gcgcttggtg tagcggtcct tggcgtcgac
     2761 ggcgaggatc agcccctgga agacgtcgaa cgacccggac aatacggtcg cgtccacgtc
     2821 gaacgcggtg acgagcacgg cgccgccgcc acccgccttg gcctcgcgga gcgtgacggc
     2881 ggcgtttgcg aatagctccg tgacggagtc gccgtcgtcg gggaagcggg cgatggcccc
     2941 gctgatggtg accggcaggt cctggctgtc cccgaagcgg aggcggacct cggcgaggtc
     3001 gcgctggacc tgctccatgg ttcgcagcag cgtccccacc gccggccctt ccaccgcgac
     3061 caggaactcg tcggggccgt accggccgac ggcgagcgag ctcgctgcga ggggccggag
     3121 gcggtcgccg accgcgctga tcgcgtcgtc accggccgcg tggccccagg tgtcgttgag
     3181 aatccggaag ttgtcgatgt ccagcagcgc gatctcgagc ccttcccccg tgcctcgcag
     3241 gcgctcgatc gcggcctcga ccaggtcgac cagctcgccg tggttgagca tgccggtgaa
     3301 cgggtcgcgg cgcgtagcct cgaggagggc gacggtctgg cgcgtgatcc gggcctgcgc
     3361 ggaccggaag acgaggtaca ggacgatggc ggcggccacg gccgcggaga cggtcacgag
     3421 gacgacgtcc cggcgggcgg catcgatcgc ggccatgatc ggggcggcgt cgcgccacac
     3481 ggccacgacg ccggcgacct ggccgtcgcg aagcagcggc agatactcgc ggaccatggt
     3541 ctcggcgccc agctcgcccg gaccggcctc gctgaccgcc gccgcggtga tgcccacggc
     3601 tgtctggccc gcgatggcgg tcgcccagtc gggagagcca gctgcgcgga agcccgcgat
     3661 cggcgcatcg ctggcgacga tggtgccgtc gggccgccgg atctcggcgc ggaggatgcc
     3721 gcgcggcgcg acgaacgcct gcagcccggc ctggagcgtg gcgaggcgac gtacgtccgg
     3781 acccggcgcc tcgagatctg cgggcacgag aaggtcgtcg acgaacccgc ggacgatctg
     3841 ggtgtcggca gtcacggcct ggttcagcgc ggacgtcgag acgtgcagcg acgcgagcat
     3901 gacctgtgcg ctagcggtga cgcccacgat cacgaggaac accccgtaga cgatcagcag
     3961 caggacgggg cggggacgtc gcaccgccgt ccgcgaacgt gtcgctgttg tcgatgtcgc
     4021 gggtctgatg tgggttccgg ccgaccgcgc ctgggacgtc acggagctcc tcgaattggg
     4081 tgcagctgcc ggacgcaacg tatcggcccc cagcacgcga tcgggatgac ccaaaagtca
     4141 cgacggtgcg gaggcgcacc ggcccgtgtt ggtctggggt cccgggcgtt ggctcaggcg
     4201 gcgctgaagc cgccgcgcag cgagaggcgg gaccccgcct cgtagaacgc aagcgcgcgg
     4261 cgcatctgct cgtcgcgccg atggcgcccc tcggcgacgc gccacaggac gcgcgcgagc
     4321 tcgtcgactc gcgcgaacag ctccgcctca ctgcgcggcg tcgggatgtc cgcataggtg
     4381 cgatggaccc gcggccaggt gacggcctcg gacgggaggg tcgggatcac cgagaacacg
     4441 atccgagcgt ggccggcggc gaggatctgg cccgtgtccg cgggccggaa cggcggacgg
     4501 ttgacggacg cctcggcggc cgcggcgacc gcgtcgggcc cggagcgcgg cacctccagg
     4561 atgccctcgc gacggacgag gtcgcgcagc tcggcggcct caaactggcc ggccttcgcc
     4621 agggcggtga ggccggcccg gacggcatcg acgtgggcca gcccgtcgtc ggcgatgacc
     4681 gcccggacca tctcgccgtc acccgatcgc tcgtggacca ccgcgaccag cgccgcacgc
     4741 gtccggcggc atgtctcgac cagcccgtcg aaggcggcga actgcgtcgg ttcccgcgcc
     4801 tcaccgaacg accggccgcc gtccaccgct atcccccgcc ccagctccag cccgggatcg
     4861 cctcgatcgc ctgttcgtcg gcaggatcga gctccttgtc gtggtggaga tcgcgcacgt
     4921 agccgagcca gtcgcccagc gggaactcgt cgtccgtcat gtagtccggc cgcggcatgc
     4981 ccgcgccggc ggcgatgtgg gcggccatca ccggcacccc ggcctcggcg aaggcgtcgt
     5041 acgtctccca gccgtcgccg gcctcgcgga tcggcgcgtc gtcggcgaag caccagatcc
     5101 agtcgtcgcc cggggacacg cccttcatca ccgcgtgtcc gacgccctgc cagtggccgc
     5161 gcatatgccg cctcgggctg ttgtcgcagc acagggtctg gccgcagacc aggcactggc
     5221 gcaagtggac ccagcgggtg ccttcggcga cgcaggcttc gcagacctcg aggtgttcgc
     5281 ccacggggtg catggtggcg gcgtgctcac agcgctggcg catcggcatc cttcgcttcc
     5341 cggcgcccgg gccggtttgg ttgtgcgtgt cataccacca tggggtggct cgtgcagcct
     5401 gacctgcgta ctaggggtga tcgcgcgcgc cgctcccgat ccgaagatcg cctggtgtaa
     5461 cgaatcctcc accgaggcgt cctcctgccg accggggggt tgagggatgg aggccgtcac
     5521 aatgcgatcg cacgctcgag gccaggtccg gctcctgttg ctcgcgatgg tcgtggccgc
     5581 cgtcgccgta cccgtcgcgc ccgccgagcg gacgaacgcg gcaacgggcg tgagcggggt
     5641 cttcatcgac agcgagcccg gggagagcgt cggcgagggc cggcaggtcg tgtacgacgc
     5701 cggcaactcc agcttcggtt tcatgagcgg gtccgacgag tcgctctggg tcgagctcgg
     5761 cacgactgac tcgttcggac actgggtgat gatgttcgcg cctgcgccgg gcgggacgct
     5821 cgtggtgggc acctatgaca acgccgtcgg tggctcattc ccgatgctgt ccgtgagtca
     5881 cggggggctc atcggatgct cgagcttcgg cggatggttc acgatcaacg agtacgcgca
     5941 tgaccccggt ggcgtcatca ccgctctgtc gctcaccttc cgccaggtct gccagccggg
     6001 cggcaagtcg ctctacggcg aggtccgcta ccaagcggcg aacgggttca aggcggcaac
     6061 gctctcgccg acgtccagga ccttcgactc ggcactcgtc ggcgacacgg gcgaccagca
     6121 gaccttcacg ctcacctccg cgggccagct cgacctcgag gtcggttcgg tctcgatggc
     6181 cggcgaggcg gcggcagatt acgaggtcgc cggagactgt gcgccgggaa cgctccctgc
     6241 cggttcgtcg tgcacgatga cggtcacctt cgctcccgag acggacggga cgcgtcaggc
     6301 ggtgctctcg atcgccgacg acaccgtccg aggcgagcga tcgagcgccc tgtccggcgt
     6361 cggtcgcctc ttcatctggg gatcaccggg ggccgccggt ggcaactacg cgtggaactg
     6421 gggccatgcg ctcgcccgga cgtcgcccgc gtcgggcggc ccatatctcc acgccctgta
     6481 cacgaccaac cgcgtcgggg gcaagtggat caaggattcg ggaccgtatg caggcgtgac
     6541 ctacatccgg agctccgaca acgggacctc gtggggctca cccaggcgaa tcaatcccag
     6601 ctcgcaacac ggtgaccgcg aggcggtcgc tgcatcgggc aactacgtct acgtgacctg
     6661 ggtccgggtg aagaagtggg tgcatttcaa gggttcggac ccgcgcgccc tgtacatccg
     6721 cgtgagtccc gacaacggcg cgacgtggaa gccgatcaag cggctgacct cggcgtcggg
     6781 gcgtgttgac gtgagctccg tcgctgcgtc cggctcgtcg gtctacgtcg cctatacgaa
     6841 ctcgtcgacc ggcgacgttc gcctcgccgt cagccgcaac cacggcagga cctggcacac
     6901 gaccaaggtc ggcacgacga agctcaaggc ggcgcaaggc cgtgtcgggc tcgccgtggt
     6961 cgccgcatcc gggagcaacg tcggcgtcgc ctggagatcc ggcttgcagc aggtcaaggg
     7021 cagggtctcg acgaactcgg gcaagagctg gaaggcggcc gttgagctcg gcggcaacag
     7081 cgacagcccg acggtcgccg ccgcgggctc gcgcgtcgtg ttcgcctgga tgcagaacct
     7141 ggacctgcgc gtcaggacct ggaacggcgc gtggagccct gagatcagag tggacgcggg
     7201 cggtgacctc tcggggcagt tgccgatcaa tgtcacggtt gcgctcaacg ggtccagccg
     7261 catcgggctc gcctgggccg gctgcctgac aggctgtgac gacacctaca atgccaacag
     7321 ccgggtcgga ctcttctggc ggacgtctcg cgacggcggc gccacctggg agccccgcga
     7381 caccctcgcg accggcgacg aggcgagtag tcgccacatc aacgacaccc cgtccatcga
     7441 ctggtcggtc acggatgccc cggtggtgct gttcaatggc tggtcgccgg gaacgaacga
     7501 ctaccggctg tacctgatgc aaccgagctg agcgtcggcc gctggcggag cgagtgggag
     7561 tcgattccgc atcggccgtt cgcgccaagg attggtggat cacctgtccg gctccaacca
     7621 tcgtgcgtcg ccatggagcc tggtcgctgg ttcgatctct gccgcgctcg actcgagcgt
     7681 cacgtaccct cggtgacggg gcgtcgaggc ggaggagtgc tgtggaccac gacgatgcgg
     7741 ttcggatgct caaggcgatc gctacggcgt tcgacgagca cgaccttgac gggatcatgg
     7801 tccacttcgc cgacgatgcg atcttcgagg cgccgcgtgg ggctgatccg tggggcaccc
     7861 ggttcgtcgg aagcgatcag gtccggcagg ccttcgccgc gcggttctcc gggatccccg
     7921 acatccggta ccagcaggac gagcacttcg ccgacggcga tcggggtgcc tccgagtgga
     7981 ccctgtccgg aacgacgacc gacggtcagc gcatcgaggt ccgcggctgc gacctgtgga
     8041 ccttccggga cggaaccgtc gtcaagaagg actcgttctg gaagatccga accggcccgt
     8101 agggctggcg gagcgggtgg gattcgaacc cacggaagct tgcgcctcag cggttttcaa
     8161 gaccgccgcc ttcaaccact cggccaccgc tccgcgggcg aggataacag cgcgccgtcg
     8221 ggcgttcagt ggtggccgga cacaaccgcg tggccgaggc cgcgtgagat cagccagcgg
     8281 ttgaccggca gcgcgacgag gaacgcgacg aacagcgcca gggcgaggct gccccagaac
     8341 aggccatcga gcagccctgc cgccatcgcg ccggggatcg cgagcacgat cagcgtgtcc
     8401 atgatctcca tgaccgtgat cgagacggtg tccgccgcga gggcgatgcg gcccgcccga
     8461 cgcaagccga tgccgctggc gatcagcggt cgcatggtca gcgcatagcc gaaggtgaag
     8521 gcgaggacga tcgcgagcag caccgacggc aggtccggga gcccgagcgc ggacgcgatc
     8581 acgaggccca acacctcgcc gatcgcgcag cccgtgaggc agtggacggt cgcgtcgaac
     8641 gcggtgcggt tgaggttcgc ctgcgcgtgt tccatactcc ctaggagtat acgggcgcgt
     8701 caagcgagcg ccggttcggg cgcctcggga tatgctccgc ccgcggcagg aacggtgcgc
     8761 cgggcccgcc ggagaaccgg ttgggcatac gggagccgac gtgtttcgcc gaccgcgcaa
     8821 cgggaccaac gggagcggcg gccaggaggt cccgcccccg ggccaccagc atctgatcga
     8881 cctgcgctcc gtggtcaagg actacgtgac ggacgcgggg ccgttccgcg cgatcgacgg
     8941 cgtcgacctc caggtggaca agggcgagtt catcgcggtc gtggggcggt cgggctgcgg
     9001 caagtcgacc ctcatgaaca tgatcaccgg gatcgaccg
//
LOCUS       DBNHXO010000529         6448 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1366171, whole genome shotgun sequence.
ACCESSION   DBNHXO010000529 DBNHXO010000000
VERSION     DBNHXO010000529.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1366171"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..113
                     /locus_tag="AC162A_10710"
     tRNA            29..113
                     /locus_tag="AC162A_10710"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63..65,aa:Tyr,seq:gta)
     gene            complement(171..833)
                     /locus_tag="AC162A_10715"
     CDS             complement(171..833)
                     /locus_tag="AC162A_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011357.1"
                     /translation="MTPRRALLLLATFALVLAAACSAPIAAVPPAPSLSPVSSAPDPT
                     PIPVPSVTPAPTATPTPPVDEGNVEGDVYPELTVESLDAETLRVSLDDPAAKAWRLVI
                     AGGGDLRYDRLEIEVVTSDVSPLITAREIRDSEVVSEMDLSGYAYGNGAAGGCHATLG
                     VCIDSDGFRLPEDGNGRFRFRLSIADVTGPLAITGSTADWPGEPFILGRWVDTDTLDW
                     SA"
     gene            complement(984..1757)
                     /locus_tag="AC162A_10720"
     CDS             complement(984..1757)
                     /locus_tag="AC162A_10720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR5011358.1"
                     /translation="MATETDALARLPGGELRYVLRRSPRSRRLRVTIVPDRGVVVSVP
                     VAGRRGWARPEPMVTAFLAERESWIRRHLDRQEATQARLEARPGLEDGRLVPYLGLPH
                     RVRVVAAGPGYRASRVERVGGDDGDELLVERVARDRRPTAVILEAWFRARARASLDAA
                     IGRHGPALGVAPARITIRDTTSRWGSCSRKGSLSFSWRLVLAPPEALDAVAAHELCHL
                     RVFGHGPAFITLLSSRVPDHAAWRRWLRKHSAELHAAID"
     gene            complement(1769..3397)
                     /locus_tag="AC162A_10725"
     CDS             complement(1769..3397)
                     /locus_tag="AC162A_10725"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR5011359.1"
                     /translation="MAFDLLIRGGLLIDGTGTPARPADVGVTGDRVTAIGDLSAIGDS
                     DVATVIDTTGHVVSPGFVDPHGHSDGSVLLDGALASHLRQGFTTQLSGNCGYTYAPMA
                     GVVRAMLDADFTTLGLDPGWTTFAEYLDEVERQPLAPNVAFLVGHGTVRWAALGPDGH
                     APSPRELAAMVRHVDEALEAGAVGVSSGLIYAPGVHAAPDEVAALVAVVARRDGLYAT
                     HMRNESAGVLDSLDEAISTTRLAGELAGRPARLQVSHLKAGARSVWGTADALVERLEA
                     ARRDGLDVAADQYPYTAAATTLSIILPPTILALSIADAEAAIRDPEARRAIREQMREG
                     SSGWENVTLDPGWGSIVISRSASRPEWDGRSIQEIADDLGSDPVDLALDVLADDRLSV
                     DIVIHCMAEPDVEAIMRMPWIAVCTDAEGRRPGHPILGEGVPHPRTYGSTARVLGDYV
                     RTRGVIDLETAVAKLSAVPAARVGLRDRGVLREGAYADVVVFDPATVADLATYERPAV
                     HPAGIRDVIVNGRVAVLGGRETGERPGRLLRRRG"
     gene            complement(3419..5269)
                     /locus_tag="AC162A_10730"
     CDS             complement(3419..5269)
                     /locus_tag="AC162A_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012652.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HIR5011360.1"
                     /translation="MSLLQRVERAQQAANEAANQGAGAPKPPPTSTSTAIVPVAAPSE
                     QRTAPRTPARELLLREIRILLQAAAVNAFDTLVDVPASEVRTRIEGVFDSVVKTHGFA
                     VTRPERDRLVDEMIDDVTGFGPIEPLLHDESITEVMVNGPDHIYIERGGKILRVDTTF
                     LNDEHVLRVIDRIITPMGRRIDETSPRVDARLPDGSRVNAIIEPLSLIGPVITVRKFS
                     TRPYTVDDLIAFGTATPEMFDFVRASIEARLNLFVSGGTGSGKTTTLNVLSQFIPNDE
                     RIVTIEDAAELQLRQEHVITLESRPPNLEGEGEITIRNLLRNAMHMRPDRIIVGECRS
                     GEALDMLQAMTTGHDGSLSTGHANSPRDMLRRLETMVLMTGYALPLRAIREQIASAVD
                     LIVHTARLKDGSRKIVTMTEVYGIEDDEILTQDIFEFRQTGVRDGKIEGELVPTGHRP
                     TFMPQFARNGVELPPDEFGIPSEKDAKPTASKVLKSRYGIGAPPEGTDHKVLELGRGR
                     AVTAGGMVYVSSVGPIDLESGQVVLGSIKEHTRQCMHNLKEKLEASGSSLDRIAWASW
                     SLREPSDFDAFNEEWVRWFPGDAPVGQGTLMPPLQRRAGFRLSLGVIAEA"
     gene            5740..>6448
                     /locus_tag="AC162A_10735"
     CDS             5740..>6448
                     /locus_tag="AC162A_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011361.1"
                     /translation="MIAVIAAVFGYQYWDQQQNYKAGHEAYLAADCAGAIVPLRKAAD
                     GSGDTATQAQAELQECQALLEGSDLIAGGNPADGLLAYSEFVLKYGDGPRADAAVAGG
                     RQAIDDGPPESVASVDVCDSLDTLEEQGFVAGEDEALPPLLYACGQTYEASAAYAAAI
                     VVLGRFLTSYPDHALTDSVKDAFARVTVADAKAAGAGSLPEPDHVGPSEAGGDLATVI
                     IQNSSPESMGIVFSGPDV"
BASE COUNT          906 a         2263 c         2370 g          909 t
ORIGIN      
        1 gttcgagtcc cctcatctcc accatcgtgg gtcggttggg caggtggtga gcccaaaggt
       61 ctgtaaaacc ttcgccttcg gctgtggcgg ttcgattccg ccccggccca ccagcatccc
      121 tggagacgac gcccggtctc cgcacgcccg cgaccgggcc gatcagcgga ctatgccgac
      181 cagtcgaggg tgtcggtgtc gacccatcgt ccgaggatga acggctcgcc cggccagtcg
      241 gcggtgctgc cggtgatcgc cagcgggccg gtcacgtcgg cgatcgagag gcggaaccgg
      301 aaccggccgt tgccgtcctc cggcaggcgg aacccgtcgg agtcgatgca tacgccgagc
      361 gtggcgtggc agccacccgc ggcgccgttg ccgtacgcgt agcccgacag atccatctcg
      421 ctgaccacct cgctgtcgcg gatctcgcgc gcggtgatca acggcgagac gtcgctcgtc
      481 acgacctcga tctcgagccg gtcgtagcgg aggtctccgc cgccggcgat gaccaggcgc
      541 caggccttgg cggccggatc gtcgaggctg acccggagcg tctcggcgtc aagcgactcg
      601 accgtgagct cgggatagac atcgccttcc acgttgccct cgtcgacggg tggggtgggg
      661 gtggcagtcg gggccggggt gaccgacggc acggggatcg gggtgggatc gggggcgctc
      721 gatacggggc tcagtgacgg cgccggcggc accgccgcga tcggcgcgga acaggctgcc
      781 gcgagcacga gggcgaaggt ggccagaagg aggagggcgc gacgcggggt caagggaacc
      841 tcaggcattc gggcgatgtg agctcggggc atccgtacga cgcttgagcg catcgttatg
      901 gttcccccgc cggcccacga aatcgagtga cattcgtcca tgccggccgg gctgttcgtc
      961 gcgcgacgag ggccggcggc gggtcagtcg atggcggcgt gcagctcggc cgaatgcttc
     1021 cgcagccagc ggcgccaggc ggcgtgatcg gggacgcgcg aggacagcaa cgtgatgaat
     1081 gcagggccgt ggccgaacac ccgcaggtgg cacagctcgt gggcggcgac ggcgtcgagg
     1141 gcctcggggg gcgcgaggac gagccgccag ctgaacgaga ggctgccctt gcgcgagcag
     1201 ctcccccagc ggctggtcgt atcgcggatc gtgatccgcg cgggtgctac cccgagcgcc
     1261 gggccgtgac ggccgatggc ggcatcgagg gacgcgcggg cgcgggcacg gaaccaggcc
     1321 tcgaggatca ccgcggtcgg acgccggtcg cgcgcaaccc gctcgaccag cagctcgtcc
     1381 ccatcgtcac cgccgacgcg ctcgacccgc gacgctcgat agcctgggcc ggccgcgacc
     1441 acgcgcaccc ggtgcgggag cccgaggtag ggcacgaggc ggccgtcttc gagccccggc
     1501 cgcgcctcga gtcgcgcctg cgtcgcctcc tggcgatcga ggtggcgccg gatccacgac
     1561 tcgcgctccg ccaggaacgc ggtgaccatg ggctcgggtc gcgcccagcc gcgccggccc
     1621 gcgacgggga ccgagacgac cacgccgcgg tcggggacga tcgtcacgcg cagccggcgg
     1681 gatcggggcg agcggcggag cacgtagcgc agctcgccgc cgggaaggcg cgcgagcgcg
     1741 tccgtctcgg tcgccacggc ggcgagcgtc atccccggcg ccgcaggagg cggccggggc
     1801 gttcgccggt ctcgcgcccg ccgagcaccg ccacccggcc gttcacgatc acgtcgcgga
     1861 ttcccgccgg gtggacggcc ggccgctcgt aggtcgcgag atcggcgacg gtggcggggt
     1921 cgaacacgac gacgtcggca taggcgccct cgcggagcac gccccggtct cgcaacccga
     1981 cgcgcgccgc cggcacggcg ctcagcttcg cgacggccgt ctcgaggtcg atgacgcccc
     2041 gcgtccggac gtagtcgccg agcacgcgcg ccgtcgagcc ataggtgcgc gggtggggca
     2101 caccctcgcc caggatcggg tggccgggac ggcgaccctc ggcgtcggtg cagacggcga
     2161 tccagggcat ccgcatgatc gcctcgacgt ccggctcggc catgcagtgg atgacgatgt
     2221 cgaccgagag gcgatcgtcg gcgaggacgt cgagggcgag atcgacggga tcactgccga
     2281 ggtcgtcggc gatctcctgg atcgaccggc catcccactc cggccgcgac gcgctgcgcg
     2341 agatcacgat cgacccccag ccgggatcga gggtgacgtt ctcccagccc gagctgccct
     2401 cgcgcatctg ctcgcggatc gcgcggcgcg cctccgggtc gcggatggcc gcctccgcgt
     2461 cggcgatcga gagggcgagg atcgtgggcg ggaggatgat cgagagggtg gtcgcggccg
     2521 cggtgtacgg gtactggtcg gccgcgacgt cgaggccgtc gcgccgcgcc gcctcaaggc
     2581 gctcgaccag cgcatcggcc gtgccccaga ccgagcgcgc gccggccttg aggtgggaga
     2641 cctggagccg cgcgggacgg ccggccagct cgcccgccag gcgggtcgtg gagatcgcct
     2701 cgtccagcga gtcgagcacg cccgccgact cgttgcgcat gtgggtggcg tacagcccat
     2761 cgcgccgggc gaccaccgcg accagcgcgg cgacttcgtc gggcgcggcg tggacgcccg
     2821 gggcgtagat gagcccgctg gagacgccga ccgcacccgc ctcgagcgcc tcgtcgacat
     2881 ggcggaccat cgccgcgagc tcccgcggcg agggcgcgtg gccatccggc cccagagcgg
     2941 cccagcggac ggtcccatga ccgacgagga aggccacgtt cggcgccagg ggctggcgtt
     3001 ccacctcgtc gaggtactcg gcgaacgtcg tccaccccgg atcgagtccg agcgtcgtga
     3061 agtcggcgtc gagcatcgcc cggaccaccc cggccatcgg cgcgtacgtg tacccgcagt
     3121 tgcccgacag ctgggtcgtg aaaccctggc gcaggtggct cgcgagcgcg ccatcgagca
     3181 gcacggagcc gtccgagtgg ccgtgggggt cgacgaagcc gggcgagacg acgtgtccgg
     3241 tcgtgtcgat gacggtcgcc acgtcgctgt cgccgatggc cgagaggtca ccgatcgcgg
     3301 tgacccggtc gccggtgacg ccgacgtcgg cgggcctcgc gggggtgccg gtgccgtcga
     3361 tcaacaggcc gccgcgaatc aggaggtcga acgccatccc cgcgagggta cctgcgaatc
     3421 aggcctccgc gatgaccccc agggagaggc ggaacccggc ccggcgctgg aggggcggca
     3481 tcagcgtgcc ttgcccgacc ggcgcatcgc ccgggaacca gcgcacccac tcctcgttga
     3541 aggcgtcgaa gtccgagggc tcgcgcagcg accagctcgc ccaggcgatc ctgtcgagcg
     3601 agctccctga ggcctcgagc ttctccttga ggttgtgcat gcactggcgc gtgtgttcct
     3661 tgatcgaccc gagcacgacc tgccccgact cgaggtcgat cgggcctaca gacgacacgt
     3721 agaccatgcc gccggcggtc accgcgcgtc cccggccgag ctcgagcacc ttgtggtcgg
     3781 tgccctccgg cggcgccccg atgccgtagc gcgacttgag caccttcgac gccgtcggct
     3841 tcgcgtcctt ctcactcggg atcccgaact cgtccggtgg cagctcgacg ccgttgcggg
     3901 cgaactgcgg catgaaggtc ggccggtggc ccgtcggcac cagctcgccc tcgatcttcc
     3961 cgtcgcgaac gccggtctgc cggaactcga agatgtcctg ggtcaggatc tcgtcgtcct
     4021 cgatcccgta cacctcggtc atcgtcacga tcttgcgcga gccgtccttg aggcgggcgg
     4081 tgtgcacgat caggtcgacc gccgacgcga tctgctcgcg gatcgcccgc agcggcagcg
     4141 catagccggt catcagcacc atcgtctcga ggcggcgcag catgtcccgc gggctgttcg
     4201 catggccggt cgagagcgag ccgtcgtggc ccgtcgtcat cgcctggagc atgtccaggg
     4261 cctcgcccga gcggcactcg ccgacgatga tccggtcggg ccgcatgtgc atcgcgttgc
     4321 gcagcaggtt gcggatcgtg atctcgccct cgccctcgag gttcgggggc cgcgattcca
     4381 gggtgatcac gtgctcctgg cggagctgga gctcggccgc gtcctcgatc gtgacgatcc
     4441 gctcgtcgtt ggggatgaac tgcgacagca cgttgagcgt ggtggtcttg cccgacccgg
     4501 tgccgcccga gacgaacagg ttgagccggg cctcgatcga cgcccggacg aagtcgaaca
     4561 tctccggcgt ggccgtgccg aacgcgatga ggtcgtcgac ggtgtacgga cgggtcgaga
     4621 acttgcggac cgtgatcacg gggccgatga gcgagagcgg ctcgatgatc gcgttgacgc
     4681 gcgagccgtc gggcagccgc gcgtcgacgc gcgggctcgt ctcgtcgatc cggcggccca
     4741 tcggcgtgat gatccggtcg atcacccgca ggacgtgctc gtcgttgagg aacgtcgtgt
     4801 cgacgcgcag gatcttgccg ccgcgctcga tgtagatgtg gtcggggccg ttgaccatca
     4861 cctcggtgat cgactcgtcg tggagcaacg gctcgatcgg gccgaagccc gtcacgtcgt
     4921 cgatcatctc gtcgaccagc cggtcgcgct ccggccgggt cacggcgaaa ccgtgcgtct
     4981 tgaccacgct gtcgaagaca ccctcgatcc gggtccggac ctccgatgcg ggaacgtcga
     5041 ccagcgtgtc aaacgcgttt accgccgccg cctggagaag gatccggatc tcgcgcagca
     5101 gcagttcccg ggccggggtc cgcggtgcgg tgcgctgctc gctcggcgcc gcgaccggga
     5161 cgatcgcggt ggacgtcgat gtcggaggcg gcttcggggc gcccgcgccc tgattggcgg
     5221 cctcgttcgc cgcctgctgc gccctctcga cccgttggag aagcgacatg cgctcgatcc
     5281 cctctggttc gcgtcgtgta ctgccggctg atcgtccggg cgggttcccg ggcgccggct
     5341 tcggcccccc aaattcgccg agatcgcgca gaggataacc ccatcgcggg cattcgcggt
     5401 tgctggggtg caccagaact ggtggactgt ggtccggtcg gggccggtcc ggaggggcca
     5461 gaggcggcag tagaatgccg ctctgggccg ccgggccccg ggggaaggag ccacatgacc
     5521 gccggattcg atccgccgcc gccgccggga gcgaatccgg gccccttgga ccctgccgtt
     5581 ccggcctcgc cacctcctcc gccggggcag cccggtctgg ggccgacgcc gttttcggcg
     5641 gacccggtcg gagcgccgac gtatccgccc gaaggcggcc agcccgcggt cgccgcgccc
     5701 aggaagggcc ggttccgcct ccgatggctc gtcctcgggg tgatcgccgt gatcgccgcc
     5761 gtgttcggct accagtactg ggatcagcag cagaactaca aggccggcca cgaagcctat
     5821 ctggcggcgg actgtgcggg cgcgatcgtc cccctgcgca aggccgcaga cggcagcggt
     5881 gacacggcga cccaggccca ggcggagctc caggagtgcc aggcactgct cgagggatcc
     5941 gacctgatcg ccgggggcaa cccggccgac gggctgcttg cctacagcga gttcgtgctc
     6001 aagtacggcg acggcccgcg ggcagatgcc gccgtggccg gtggccgcca ggcgatcgac
     6061 gacggcccgc ccgaatcggt cgcgtcggtc gacgtgtgcg actcgctgga cacgctcgag
     6121 gaacaagggt tcgtcgccgg cgaggatgag gcactgccgc cgctcctgta cgcgtgcggc
     6181 cagacctacg aggcctccgc ggcctatgcg gcggccatcg tcgtcctcgg ccggttcctc
     6241 acctcctacc cggaccacgc gctgacggac tcggtgaagg acgccttcgc gcgggtgacc
     6301 gtcgcggacg ccaaggctgc cggcgccggc agcctccccg agcccgacca cgtcggaccc
     6361 agcgaggcgg gtggcgacct ggccacggtg atcatccaga acagctcgcc cgagtcgatg
     6421 ggcatcgtgt tcagcgggcc ggacgtcc
//
LOCUS       DBNHXO010000530         8534 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_175156, whole genome shotgun sequence.
ACCESSION   DBNHXO010000530 DBNHXO010000000
VERSION     DBNHXO010000530.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8534)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8534
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_175156"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..3031)
                     /locus_tag="AC162A_10740"
     CDS             complement(170..3031)
                     /locus_tag="AC162A_10740"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5011362.1"
                     /translation="MHGSGPLVCPVLVGRDDLLALADRRRAQAAGGRGGLLFLAGEAG
                     IGKTRLLGSIERRAAADGFRVLRGAAFPRDLEVAGGVFLDLARSMMRDPSTLDAGAAL
                     ATCLAQPDPVEPGDRHRARRILVLDVVDLVAGIASSGRAMVSLEDLHWADDLSLEVLG
                     VLARRLADLPLLVVATYRSDQLYPRVPMREWRARLLAGRLAEEARLARLDLDGTATMT
                     TLILGAALPAPRDLVAAIHQRSDGVPLHVEELLGVIRAEGMPAGVVPDTLDEAVAQRA
                     VRLSKPARSVAEAAAVIGRTFDVDLLATVLGRAQDRLSAPLAELAREFFVVPAAEAGR
                     LDFRHAIIRDALYGSITPAARRRLHGRVADAAADDPRFGPAFRSVHLEEAGRGAEAFV
                     QAVEGGRRAAALSAHREALELFTRAWRNAPADAPPTDAAMLLAELATEAAATDDNELA
                     DRALADASDRFREAGDPLAAASLVPQQVAVRHLLGDDFAARTARLTAALASMESLPDD
                     HAARAVRARLLGGLSAANMLDRRLELAIAYGRTGRALAIDVGDLPTELDAAASVGSSL
                     VFAGQMQEGWALLESTVIAARDAHLEAQAARAYRMLGSCASVLVEYERADRWLRDGIE
                     YAERVELWNHRHYMAAHLAHVRWATGDWVDAERLATHALSDGRGGITTRITALHVLGF
                     LALGRGDLAAADEALDEALALGRQMAELQRLSPALWGLAEAAQLRGDTAAAIAWCLQG
                     EAASAAVDDAAYLFPFLVTGTRAHLAAGDPTGATAWTERVSTAVIRRGIPGTLPAVDH
                     ARGLLDLARGNTAHARTSLAVARDGWRARGRAWEGAWAAIDLATALARSKRAADARVL
                     IDEVRSTAAALPSATLVAGATALDHRLRTRAAAAPWDPLTAREFEVARLIAEGRTNAE
                     IADRLGVSTRTVTSHVEHVLAKLGAARRAEIAAWATRIG"
     gene            3207..3455
                     /locus_tag="AC162A_10745"
     CDS             3207..3455
                     /locus_tag="AC162A_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014693826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO4226 family nickel-binding protein"
                     /protein_id="HIR5011363.1"
                     /translation="MAKFMDVHDGFFGVTAEQLREAHDADLAIEGDEGVHFERAWLDP
                     DAGKVFCLATGPSKEAVMRIHERAGHPTPEVYELTVEV"
     gene            complement(3489..6386)
                     /locus_tag="AC162A_10750"
     CDS             complement(3489..6386)
                     /locus_tag="AC162A_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5011364.1"
                     /translation="MSRIGPLLSPLLVGRDDLLHLADRRIAEAAAGRGQLLLLAGEAG
                     IGKTRFLYAIINKATAAGFAYSKGDLAPHDRLVPLASILDLARAMGTDPSSFGTTGEE
                     ILRMQGGQGGDHLGSRRLLVRDVAERIASSLDKPTVLAFEDLQWADELSLEVIGELAR
                     LVRDRPVLLIGAYRVDEFPVGSLHREWRARLLSQRLGEEARLASLTYDETALVTTLIL
                     ATGLPAPREVVNAVYERTDGIPLHIEELLGAMSDEARSDGRAIRNAEVPDTIEDAILA
                     RFAHLSEESQAVARAGAVIGRCFVPDVLAGIMDRPVAELDGALEELVASSFLHPFEYL
                     DQGYYDFRHQLLRDALYASVRPSDLRRLHARAAEFGAQLSGASEIHASVHFERAGLRA
                     QAFRTALAGAEAAGALSSRQESYELYARAVANVPEGLTPLELAALYDGYSNAAFAVDD
                     VPVIEQTTRLARQSYLDAGRPLDAANMLIALASVARRDVRPHDERVRLLAQADAELAA
                     LPPSPERAYALSDLRVSEAVEAFDDGRLDAAVDLLAEARQHRHDSGDPDTGDIDYIAA
                     SVQVLAGRRDAGLAEMLRVARNARDHRLESTGVTAFRWAAAYAIRVMDYPSASAGLGE
                     GLRYADEIEQSYCRHVMAATSAHVAWAAGDWDDAVLTAEIELVERGSRRGTLGSRDAL
                     GFVSFGRGDADRARMLFEASLEIGGPTGEVELILPALWGLAETALVASDAGEAARRCE
                     EGHELAVAGGERALLVPFVVTGVRAYLAAHQPDEAARWLRRVTEALGDWGGPADVARR
                     HADGLLHLAAGSTVAARADLEAAIAGWDAIGRVWESTWARLDLAMALVRAGRHADALP
                     LLAAVRAAAERLRSDPLLRRAEELAGVVRSRGAIEEPWRPLTVREFEVAGLVAEGMTN
                     PEIAEALGLSPKTVSAHLEHILAKLGAMRRAEVASWVSTIRPKAGVGGGRR"
     gene            6491..7507
                     /locus_tag="AC162A_10755"
     CDS             6491..7507
                     /locus_tag="AC162A_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5011365.1"
                     /translation="MRIGMIGTGLMTLYHLTSMVGRDDTVIAAMCEPSAAAYEAAAAR
                     VEAAGAPRPPNEPDWRRFLDVTARELDLDAVMIVTPHALHFEQASAALEAGLDVLLEK
                     PMVMTAHEAVELIRVRDRTGRLLVVGFQGSLSPQVRAAAAMIRSGDLGTILNVNALVW
                     QDWQETSRGTWRQNPALSGGGFMFDTGAHMLNTVADLAGEDVVQVAAWLENDEAQVDV
                     RGAVIARLASGALITMNGCGRAIPSLGSDIHVFCERGTLRTGIWGERLELQRHGSSAL
                     RPVRSVRPKTLWQQFLDVRSGLEPNPSPPEVGLRMALLWDAIRESSGRGGAIVVPGAR
                     AGAV"
     gene            7504..8226
                     /locus_tag="AC162A_10760"
     CDS             7504..8226
                     /locus_tag="AC162A_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR5011366.1"
                     /translation="MTTRVTVWNEFRQERSDAPVGAIYPDGMHEAIAAGLRAVDGLAV
                     RTATLDEPEHGLTDQVLAATDVLTWWGHVAHDEVSDEVVDRVHARVLDGMGLVVLHSG
                     HFSRIFRRLMGTTCDLKWREDGRRERLWVVDPSQPIADGLGESMVLAEEEMYGEHFDI
                     PAPDELVLVSWFEGGEVFRSACTWRRGRGRVVYLRPGHETHPTYFDPGMRRLVGNSVR
                     WAAGAHGERPTYGHTPPAEPPA"
     gene            8237..>8534
                     /locus_tag="AC162A_10765"
     CDS             8237..>8534
                     /locus_tag="AC162A_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014981901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HIR5011367.1"
                     /translation="MLTRATIIEAPKALLHDHLDGGLRPATIVDLAAEYGYADLPTTD
                     VDELAAWFRRGADQTSLELYLETFAHTFGVMQERDAIIRVASECAQDLAADGVVY"
BASE COUNT         1200 a         3089 c         3143 g         1102 t
ORIGIN      
        1 gcgctgcgcg aggccgcgac ggtcgagggg gccctggccg ccgccgacgt gaccggcggc
       61 acggcgcccg aacgcgtggc gctggccctg gccgacgcga gggcgcgtct cggcgactga
      121 ccggcgatcc gcggcgacgc gcggcttcag tcgctcgccg acctcggcat cacccgatgc
      181 gcgtcgccca tgcggcgatc tccgcgcgcc gcgccgcccc gagcttcgcg aggacgtgct
      241 cgacgtgtga cgtcaccgtt cgggtcgaca cgccgagcct gtctgcgatc tcggcgttcg
      301 tccggccctc cgcgatcagg cgggcgactt cgaactcgcg cgccgtgagc ggatcccacg
      361 gggccgctgc agcgcgggtc cggagccggt ggtccagcgc cgtggcgccg gctacgagcg
      421 ttgcggacgg aagtgcggcc gcggtcgacc gcacctcgtc aatgagcacc cgggcgtcgg
      481 cggctcgctt cgaccgcgcg agtgcggtcg cgaggtcgat cgccgcccat gcgccctccc
      541 aggcgcgtcc gcgcgccctc cagccgtccc gcgcgacggc cagcgacgtc cgggcgtgcg
      601 ccgtgttgcc cctggcgagg tccagcaggc cgcgcgcgtg gtcaaccgca ggcagcgttc
      661 ccgggatccc gcgacggatg accgccgtgg acacgcgttc ggtccatgcg gtcgcgcccg
      721 tcgggtcgcc tgccgcgagg tgggcgcgcg tccccgtcac gaggaacggg aacaggtagg
      781 cggcatcatc gacggccgcc gacgcggctt ctccctgcaa gcaccacgcg atcgcggcgg
      841 ccgtatcccc ccgcagctgc gcggcctcgg cgagccccca cagcgccggc gaaagccgct
      901 ggagctccgc catctggcgc ccgagggcca gcgcctcgtc gagcgcctcg tccgcagcgg
      961 ccagatcgcc ccgcccgagg gcaaggaacc cgagcacgtg aagcgcggtg atgcgggtcg
     1021 tgatcccgcc acgaccgtcg gacagcgcgt gtgtcgcgag gcgctcggcg tcgacccagt
     1081 cgccggtcgc ccagcggacg tgggcgaggt gcgccgccat gtagtggcgg tgattccaga
     1141 gctcgactcg ctcggcgtac tcgatgccat cccggagcca gcggtcggcg cgttcgtact
     1201 cgacgaggac cgaggcgcat gacccgagca tccgatacgc cctcgccgcc tgtgcctcga
     1261 ggtgcgcgtc gcgggcggcg atcacggtcg actcgaggag cgcccagccc tcctgcatct
     1321 ggcccgcgaa gaccaggctc gagccgacgc tcgccgcggc atcgagctcc gtcgggaggt
     1381 ccccgacgtc gatcgccagc gcccgccctg tccggccgta cgcaatcgcg agttcgagcc
     1441 gccgatcgag catgttcgcc gcggacagcc cgccgagcag acgcgcccga acggctcgtg
     1501 ccgcatggtc gtccggcagc gactccatcg acgcgagcgc cgccgtgagg cgcgcggtcc
     1561 gggcggcaaa gtcgtcgcct aggagatggc ggacggcgac ctgctggggg acgaggctgg
     1621 cggctgcgag tgggtcgccg gcctcgcgga accggtcgct cgcatcggcc agcgcgcggt
     1681 cggccagctc gttgtcgtcg gtcgccgcgg cctcggtcgc gagctcggcg agcagcatcg
     1741 cggcatcggt cggcggcgcg tccgccgggg cgttgcgcca cgctcgcgtg aacagctcga
     1801 gcgcctcgcg gtgcgccgac agcgcggccg cacgccggcc gccctcgacc gcctgcacga
     1861 atgcctccgc gccgcggccg gcttcctcga ggtgcaccga gcggaatgcc ggcccgaagc
     1921 gcggatcgtc ggcggccgca tccgcgaccc gaccgtggag ccggcggcga gcggccggcg
     1981 tgatcgagcc gtacagcgcg tcccggatga tcgcgtgccg gaagtcgagc cgtccggcct
     2041 ccgcagcggg cacgacgaag aactcccggg ccagctcggc gagcggtgcc gacagccgat
     2101 cctgcgcacg accgaggacg gtggcgagga ggtcgacatc gaacgtccgt ccgatcacgg
     2161 cggccgcctc ggcgacggag cgcgccggct tcgacaggcg cacggcgcgc tgggcgacgg
     2221 cctcgtcgag ggtgtcgggg acgacgccgg cgggcatccc ctcggcgcgg atcacgccca
     2281 acagctcctc gacgtgcagc gggacgccgt cggatcgctg gtggattgcc gccacgaggt
     2341 cgcgcggcgc cggaagcgcg gcacccagga tcaaggtggt catggtggcg gtcccgtcga
     2401 ggtcgagccg ggcgagccgc gcctcctcgg cgagccggcc ggcgagcagc cgggctcgcc
     2461 actcacgcat cggcacgcgg ggatagagct ggtcgctgcg gtaggtcgcg accaccagca
     2521 gcggcaggtc ggcgagtcgg cgcgccagca cgccgagcac ctccagcgac aggtcgtccg
     2581 cccagtgcag gtcctcgagg ctcaccatcg cccggcccga gctcgcaata ccggccacaa
     2641 ggtcgacgac gtcgagcaca aggatccgcc gagcgcgatg acggtcgccc ggctcgacgg
     2701 ggtccggctg cgcgaggcac gtggcgagcg cggccccagc gtcgagcgtc gaggggtcgc
     2761 gcatcatcga ccgcgcgaga tcgaggaaca caccaccggc gacctcgagg tcccggggaa
     2821 aggccgcacc ccgcaggacc cggaacccgt cagctgccgc gcgacgctcg atcgacccga
     2881 gcaggcgcgt cttgccgatc ccggcctcgc cggcgaggaa cagcaggccg ccacgaccgc
     2941 ctgcggcctg ggcccggcgc cgatcggcaa gcgcgagcag gtcgtcacgc ccgacgagga
     3001 cagggcagac gagcggtccg gatccgtgca ccgaggacgt ccttgcggga gacatggtca
     3061 gccggccggc gaccgccggc gcgttctcca ctgtaattcg cgattctgac cgtccatcgg
     3121 tcattccacc gatgtcgggt cgccccgcgt catcgacgct ggcaacagat gagcggcgat
     3181 cgccgccccg atctggagac cacgagatgg cgaagttcat ggacgtgcat gacggattct
     3241 tcggcgtgac ggctgagcag ctgcgcgagg cgcacgacgc cgacctggcg atcgagggcg
     3301 acgagggcgt gcatttcgag cgcgcgtggc tcgacccgga tgcgggcaag gtgttctgcc
     3361 tcgcgaccgg gccctcgaag gaggccgtga tgcgtatcca cgagcgcgcc ggccacccga
     3421 cccccgaggt ctacgagctc accgtcgagg tctagacgag acccggaccc cggcaacccg
     3481 ggtggcggtc agcgacggcc gccacccacc cccgccttcg ggcggatcgt cgagacccat
     3541 gacgccacct cggctcggcg catcgcgccg agcttcgcga ggatgtgctc gaggtgggcg
     3601 ctcaccgtct tcggggacag cccgagcgcc tcggcgatct ccgggttggt catgccctcg
     3661 gccaccagcc ccgcgacctc gaactcgcgc accgtgagcg gccgccacgg ctcctcgatc
     3721 gccccgcggc tgcggacgac gcccgccagc tcctcggcgc gccgcaggag cgggtcagag
     3781 cgcaggcgtt cggcggccgc gcgcacggcg gcgagcaggg ggagggcgtc ggcgtgacgg
     3841 ccggcgcgga ccagcgccat ggccaggtcg agtcgtgccc aggtcgattc ccatacgcgg
     3901 ccgatggcat cccagccggc gatggcggcc tcgaggtcag cgcgcgcggc gacggtcgag
     3961 ccggctgcga ggtgcagcag gccgtcggca tgccggcgcg cgacgtcggc ggggccgccc
     4021 cagtcgccca acgcctcggt cactcgccgc aaccagcgcg cggcctcgtc gggctggtgc
     4081 gcggcgaggt aggcgcggac gcccgtgacc acgaacggca cgagcaacgc tcgctcgcca
     4141 cctgccacgg cgagctcgtg accctcctcg catcgacgcg cggcttcgcc agcatcgctg
     4201 gcgacgagcg ccgtctctgc caggccccac agggccggca ggatcagctc cacctcgccg
     4261 gtgggcccgc cgatctccag ggacgcctcg aacagcatcc gcgcacggtc tgcgtcgcca
     4321 cgcccgaacg agacgaagcc cagcgcatcc cgcgagccga gggtgccacg ccggctgccg
     4381 cgctcgacga gctcgatctc ggccgtcagg accgcgtcgt cccagtcgcc ggccgcccac
     4441 gcgacgtgcg ccgaggtcgc ggccatgacg tggcgacagt aggactgctc gatctcgtcg
     4501 gcatagcgga ggccttcacc gagcccggcg gacgcgctcg gataatccat cacccggatg
     4561 gcgtacgccg ctgcccagcg gaacgccgtc acgcccgtgc tctcgagccg gtggtcgcgg
     4621 gcgttgcgcg cgacccggag catctcggcc agcccggcgt ccctgcggcc agcgaggacc
     4681 tggacgctcg cggcgatgta gtcgatatcg cccgtatccg ggtcgcctga gtcgtgccgg
     4741 tgctgccgcg cctcggcgag caggtccaca gcggcgtcga gcctgccgtc gtcgaatgcc
     4801 tcgaccgcct ccgacactcg cagatccgag agggcgtagg cgcgctcggg agagggcggc
     4861 agcgcggcca gctcggcgtc ggcctgggcg aggagccgga ctcgttcgtc atggggcctc
     4921 acgtcgcggc gcgcgacgct cgcgagcgcg atcagcatgt tggccgcatc gagcgggcgg
     4981 ccggcatcga ggtaggactg gcgcgccagc cgcgtggtct gctcgatcac cggcacgtca
     5041 tcgacggcga acgccgcgtt cgagtacccg tcgtacagcg ccgcaagctc gagcggcgtc
     5101 aggccctcgg ggacattggc gacggctcgc gcgtagagct catacgactc ctggcggctg
     5161 gacagcgcgc ctgccgcctc ggcgccggcg agcgcggtcc gaaaggcctg tgcgcggagg
     5221 ccggctcgct cgaagtggac cgacgcgtgg atttccgagg cgcccgagag ctgggcgccg
     5281 aactcggcgg cccgggcgtg caggcgccgg aggtcgctcg ggcggacgct ggcgtagagc
     5341 gcgtcccgga ggagctggtg gcggaagtcg tagtagccct ggtcgaggta ctcgaatggg
     5401 tggaggaacg agctcgcgac cagctcctcg agcgccccgt cgagctcggc aaccgggcgg
     5461 tccatgatcc cggccagcac gtcgggaaca aagcagcggc cgatcacggc gccggcgcgg
     5521 gcgaccgcct gactctcctc cgagaggtgg gcgaagcgcg cgaggatcgc gtcctcgatc
     5581 gtgtccggga cctccgcgtt gcgaatggcc cggccgtccg agcgtgcctc gtcggacatg
     5641 gcgccgagga gctcctcgat gtgcagcggg atgccgtcgg tgcgctcgta gaccgcgttg
     5701 accacctccc gcggcgcggg cagccccgtc gccaggatga gcgtcgtcac cagcgcggtc
     5761 tcgtcatagg tcagggacgc cagtcgcgcc tcctcgccca ggcgctggct cagcagtcga
     5821 gcgcgccact cacgatggag cgagcccacg ggaaactcat cgacccggta ggcgccgatg
     5881 aggagcacag gccgatcgcg gaccaggcgt gcgagctcgc cgatcacctc aaggctcagc
     5941 tcgtccgccc actggaggtc ctcgaacgcg agcaccgtgg gcttgtcgag ggacgaggcg
     6001 atgcgctcgg cgacgtcacg gaccaggagc cggcgcgacc cgaggtggtc gccgccttgc
     6061 ccgccctgca tgcgaaggat ctcctcaccg gttgtcccga atgacgaggg atccgtgccc
     6121 atcgccctcg cgagatcgag gatcgacgcc agggggacga ggcggtcgtg cggggcgagg
     6181 tcgcccttgg agtaggcgaa gccggcggcg gtcgccttgt tgatgatcgc gtacaggaaa
     6241 cgcgtcttgc cgatcccggc ctcgcccgcg agcaggagca gctgaccacg acccgcggcg
     6301 gcttcggcga tccgtcggtc ggcgaggtgc agcaggtcgt cccgaccgac caggagcgga
     6361 gagagcaggg gtccgatgcg actcatccgg cggagtgtag gcctgcggta cgcggacggc
     6421 atcggtcgat cgaccgatgg gcggtggccg gcgggcgacc accgcgaccc ccgtcgggta
     6481 ctctcgcggc gtgcgcatcg gaatgatcgg gacgggcctg atgacgctct atcacctgac
     6541 ctcgatggtc gggcgagacg acaccgtcat cgccgcgatg tgtgagccct ctgccgccgc
     6601 ctacgaagcc gcggccgcga gggtcgaggc tgctggggcg ccccgtccgc cgaatgagcc
     6661 ggactggcgc cgcttcctcg acgtcaccgc ccgcgagctg gacctcgacg cagtgatgat
     6721 cgtgacgccg catgccctcc acttcgagca ggcgtcggcc gccctcgaag ccgggctcga
     6781 cgtgctgctc gagaagccca tggtgatgac cgcgcacgag gcggtggagc tgattcgggt
     6841 gcgcgatcgg accggccgtc tgctggtggt cggtttccag gggagcctct cgccgcaggt
     6901 tcgcgccgcc gccgcgatga tccggtccgg cgacctcggc acgatcctca acgtcaacgc
     6961 gctcgtgtgg caggactggc aggagacgtc tcgcggcaca tggcgccaga acccggcgct
     7021 gtcgggtggg ggcttcatgt tcgacactgg cgcccacatg ctcaacaccg tcgccgacct
     7081 tgccggcgag gacgtcgtgc aggtcgcggc ctggctcgag aacgacgaag cccaggtcga
     7141 cgtccggggc gcggtcatcg cgcggctggc atcgggggcc ctgatcacga tgaacggctg
     7201 cggccgcgcg atcccatcgc tgggctccga catccacgtg ttctgtgagc ggggcacgtt
     7261 gcgcacgggg atctggggcg agcgactcga gctccagcgg cacggctcgt cggcgcttcg
     7321 gccggtccgc tccgtgcgac ccaagaccct gtggcagcag ttccttgacg tgcgcagcgg
     7381 cctcgagccg aacccgagcc cgcccgaggt cgggctgcgg atggcgcttc tctgggacgc
     7441 gatccgggag tcgtccgggc gcggtggcgc catcgtggtc cccggcgccc gggctggcgc
     7501 cgtatgacga cccgggtgac ggtgtggaac gagttccgcc aggagcgctc ggacgcgccg
     7561 gtcggtgcga tctacccgga tggcatgcac gaggcgatcg ccgcggggct gcgcgccgtc
     7621 gacggccttg cggtgcggac ggccacgctc gacgaaccgg agcacggatt gaccgaccag
     7681 gtgctcgccg ccaccgacgt cctgacctgg tggggtcacg tcgcccacga cgaggtgagc
     7741 gacgaggtcg tcgaccgggt gcacgcgcgc gtcctcgacg ggatgggcct ggtcgtcctc
     7801 cactccggcc acttctcgcg catcttcagg cgcctcatgg gcacgacctg cgacctcaag
     7861 tggcgcgagg acggccgccg ggagcgcctg tgggtcgtcg acccgtcgca accgatcgcg
     7921 gacggactgg gcgagtcgat ggtgctcgcc gaggaggaga tgtacggcga gcacttcgac
     7981 atcccggccc ccgacgagct cgtcctggtc agctggttcg agggcgggga ggtgttccga
     8041 agcgcctgca cctggcgccg cggccggggc cgggtcgtgt acctgcgccc gggccacgag
     8101 acgcacccga cctacttcga ccccgggatg cggcggctgg tcggcaacag cgtccggtgg
     8161 gcggccggcg cccacggcga gcgcccgacc tacgggcaca cgccgccggc ggagccgccg
     8221 gcgtagggga ggggcgatgc tcacccgggc caccatcatc gaagcgccca aagcgctgct
     8281 ccacgaccat ctcgacgggg gcctgcggcc tgcgacgatc gtcgacctcg ccgccgagta
     8341 cgggtacgcg gacctgccga ccaccgacgt cgacgagctg gccgcctggt tccggcgcgg
     8401 cgccgaccag acgtccctcg agctgtatct cgagacgttc gcccacacgt tcggggtcat
     8461 gcaggagcgc gacgcgatca tccgggtcgc gtccgagtgt gcgcaggacc tggccgccga
     8521 cggcgtcgtg tacg
//
LOCUS       DBNHXO010000531         1832 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2360980, whole genome shotgun sequence.
ACCESSION   DBNHXO010000531 DBNHXO010000000
VERSION     DBNHXO010000531.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2360980"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="AC162A_10770"
     CDS             <1..354
                     /locus_tag="AC162A_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011970120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR5011368.1"
                     /translation="GLIQVVGGLGPHGLLVGLFVVTVIFGQLISNTATALIMIPIGVA
                     AAGELGVDLRPVLMSICVAAAASLLTPVATPVNLIAMGAAGYRFGDYWRLGLVVLVVF
                     FIVSVFVVPIFWPFG"
     gene            399..1373
                     /locus_tag="AC162A_10775"
     CDS             399..1373
                     /locus_tag="AC162A_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011369.1"
                     /translation="MSTRTALASLLVAIALVVAVAPVVLAPAAEQAFADVAANLRPGD
                     AATAQLPPAPASPDPPPPTSAPVPSATPAATPASPPTPAATPSAPPRADKPAVRSPVG
                     GTNGRTVHLNLAGPRDYVAQTNLVQCVGASVQMMLNIIKPGVNRTAAYQHRLQVQARK
                     RSGPTPPGFVRSGAGMFGWAATLSLRGGGAYEVVGADNLRQAMRMAARAIHDQRRPVG
                     LLVWRGRHAWVMSGYQAVVDRKAPGGFRVTRANILDPLNPYGGSGWGPSPRPGSSTSV
                     EAVGRQFVRRQPRPDSAWTTFWRTIPGRAALEGKYVLVLPVATASEAG"
     gene            1373..1726
                     /locus_tag="AC162A_10780"
     CDS             1373..1726
                     /locus_tag="AC162A_10780"
                     /inference="COORDINATES: protein motif:HMM:NF016854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HIR5011370.1"
                     /translation="MAVPQFTKSPPELVERFDAAAARHPAAVRRKMFGYPALFVGGNL
                     ATGLFADTWMVRLAPADLEALIALPGAGPFAPMPGRTMKGYATLPPDVVAQDEELDAW
                     LERAITFAGSLPAKQ"
BASE COUNT          224 a          698 c          640 g          270 t
ORIGIN      
        1 gggctgatcc aggtcgtcgg cggcctcggg ccgcacgggc tgctcgtcgg gttgttcgtc
       61 gtcaccgtga tcttcggcca gctgatcagc aacacggcca ccgcgctgat catgatcccg
      121 atcggcgtcg ccgcggccgg cgagctcggc gttgacctgc ggccggtgct catgtcgatc
      181 tgcgtcgccg ccgcggcctc gctgctgacc cccgttgcga cgccggtcaa cctgatcgcg
      241 atgggtgccg cgggctaccg gttcggcgat tactggcggc tcggccttgt cgtgctggtc
      301 gtgttcttca tcgtgtcggt gttcgtcgtc ccgatcttct ggccgttcgg gtagcgccgg
      361 aaccggggcg ccgcttccgg cgtatggttc gacatccgat gtcaacgcga accgcgctcg
      421 cctccctcct cgtcgccatc gccctcgtcg tggccgtcgc cccggtcgtg ctcgcacccg
      481 ccgctgagca ggccttcgcc gacgtcgccg ccaacctgcg ccccggcgat gcggccacgg
      541 cgcagctgcc gccggcaccc gcgtcgcccg accccccgcc gccgacttcc gcacccgtcc
      601 cttccgcgac gccggcggca accccggcgt cgccacccac gccggcggcg accccctcgg
      661 cgcccccgcg tgccgacaaa cccgccgtcc ggtcgccggt cggtggcacc aacggcagga
      721 ccgtgcacct caacctcgcc gggccgcgcg actatgtcgc ccagacgaac ctggtccagt
      781 gcgtcggcgc gagcgtgcag atgatgctca acatcatcaa gcccggcgtg aaccggacgg
      841 ccgcctacca gcaccggctc caggtccagg cgcggaagcg gagcggaccg acgcctccgg
      901 ggttcgtgcg cagcggggcc ggcatgttcg gctgggccgc gacgctctcg ctccgcggcg
      961 gcggcgccta cgaggtcgtg ggcgcggaca acctgcgcca ggcgatgcgg atggcggcaa
     1021 gagcgatcca cgaccagcgg cgcccggtcg ggctgctcgt ctggcgcggt cgccacgcgt
     1081 gggtgatgag cggctaccag gcggtcgtcg accgcaaggc tccgggcggc ttccgtgtca
     1141 cgcgcgccaa catcctcgac cccctgaacc cgtacggcgg gagcggctgg gggcctagcc
     1201 cgaggcccgg ctcatcgacc tcggtcgagg cggtcgggcg gcagttcgtg cggcgccagc
     1261 cgcgccctga cagcgcgtgg acgacgtttt ggcgaacgat cccaggcagg gcggcgctcg
     1321 agggcaagta cgtcctcgta ctgcccgtcg cgacggcatc cgaggccggc tgatggccgt
     1381 gccccagttc acgaagtcgc cgcccgagct ggtggagcgc ttcgatgccg cggcggcccg
     1441 ccatccggcg gccgtgcgcc ggaagatgtt cggctacccg gccctgttcg tcggcggcaa
     1501 cctcgcgacc ggcctcttcg ccgacacgtg gatggtccgg ctggcgccgg ccgacctcga
     1561 ggccctgatc gcgctgcccg gcgcggggcc cttcgcgccg atgccgggcc gaacgatgaa
     1621 gggctacgcg acgctgccgc ccgacgtggt cgcccaggac gaggagctgg acgcctggct
     1681 cgagcgcgcg atcacgttcg cggggagcct gcccgccaag cagtaacgcc gaccgggacc
     1741 ccccgcgcat ctggggggtc gccgtatggc gcaggatcgc gtatcctccg tcgacgcccc
     1801 atcgtcgggc gataccagta cggcacactc ga
//
LOCUS       DBNHXO010000532         2664 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4941821, whole genome shotgun sequence.
ACCESSION   DBNHXO010000532 DBNHXO010000000
VERSION     DBNHXO010000532.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4941821"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="AC162A_10785"
     CDS             complement(<1..379)
                     /locus_tag="AC162A_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5011371.1"
                     /translation="MTEVAAPPATHRLFIAGRWVESVSGETFDSVNPADTRDLVGHFQ
                     AGTAADVAMAVRAAEMAFPAWRATPAPKRGEILYRFGALIAEHKERLARAMTREMGKV
                     IAEARGDVQEGIDIAFLMAGEGRR"
     gene            complement(465..1715)
                     /locus_tag="AC162A_10790"
     CDS             complement(465..1715)
                     /locus_tag="AC162A_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011372.1"
                     /translation="MVARLRPLALLVALVALALAAAPPAARADRGLARVPVHIGGDVL
                     FDEARNDGRQGGPGTWSVTEGRLTRDGLRLHVEIDPVLQGLPVQSGPAVLFAGMQAFG
                     GSGDRGLGVSIDALATVELPPCDDADCRYAADVELPTAGIPGAVDRLDKMGDFLWIEV
                     ALTLVRTYGDGSWLQVTPLAPWMDGATFSDAGRLGNVKPVGGRLFPYGLFPSDEATPI
                     PRRGGWMFTSGFDYGRVVERLRKRAGDPSTPIPGVDARLHVTIEPACLHAAHLTLHDD
                     AGDRVFDADVYDTAEIDAVVRLPAGTTWRLTLHDGGGIDFDQGRQGWGVRVGSIEAVG
                     GPLDIAATFECVTPSGEVDVVGATQASADPTSAPPRPAPTIEPSSALTNADGDRLAWL
                     GAVVAAGTAASVALLVVLGRRRSR"
     gene            complement(1766..>2664)
                     /locus_tag="AC162A_10795"
     CDS             complement(1766..>2664)
                     /locus_tag="AC162A_10795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR5011373.1"
                     /translation="DGDLYVAGGMESMNQAPFLLRKARFGYRLGNGTLEDSTVLDGLW
                     CSVEDCHMGTHAERVAIHDQVSREDQDAFALASHQHAIAAIDEGRFDAELAPVTVRDA
                     KGRETLVTVDESPRRDTTAEALARLAPAFDLPEGEDRGSATTGTVTAGNAPGITDGAA
                     ATVVASERAVEEHGLTPLARIVGYAQAEVAPKWLFLAPVQGVRGLEARTGLPIEAYDL
                     VEINEAFAAQALADGRELGFDWSKVNVNGGAIALGHPIGASGARIVATLLHELARRGG
                     RYGMATLCLGGGGSVAMAFERV"
BASE COUNT          380 a          986 c          946 g          352 t
ORIGIN      
        1 tccggcggcc ctcgccggcc atcaggaagg cgatatcgat cccctcctgg acgtcgccgc
       61 gcgcctccgc gatcaccttg cccatctcgc gggtcatcgc gcgggcgagc cgctccttgt
      121 gctcggcgat cagcgcgccg aagcggtaca ggatctcgcc gcgcttgggg gcgggcgtcg
      181 cgcgccaggc ggggaacgcc atctccgcgg cacggacggc catcgcgacg tcggccgccg
      241 tgcccgcctg gaagtggccg acgaggtcgc gcgtgtccgc ggggttgacg ctgtcgaacg
      301 tctccccgga cacggactcg acccagcggc cggcgatgaa caggcgatgc gtggcgggcg
      361 gcgcggcgac ctcggtcaat gcggctcctc gctgctggca acggtcgacg tcgatgttcc
      421 gtgccggagc gcgcatccgt caatgcgcga gccgcaggac gaggctagcg cgatcgtcga
      481 cggccgagca cgactagcag cgcgacggat gcagccgtac cggcggcgac gacggctccg
      541 agccatgcga gtcggtcacc atcggcgttc gtcaacgcgg aggatggctc gatcgtgggc
      601 gccggacgcg ggggcgcgga cgtcgggtcc gcggaggcct gcgtcgcacc gacgacgtcc
      661 acctcgccgc tcggcgtcac gcactcgaac gtcgccgcga tgtcgagcgg gccgccgacg
      721 gcctcgatgc tcccaactcg gacgccccac ccctgacggc cctggtcgaa gtcgatcccg
      781 cccccgtcat ggagggtgag ccgccacgtc gtgccggccg gcagccggac cacggcatcg
      841 atctcggcgg tgtcgtagac atcggcatcg aacacccggt ccccggcatc gtcgtggagc
      901 gtcaggtggg cggcatggag gcaggcgggc tcgatcgtga cgtggagccg ggcatcgacg
      961 cccggaatcg gggtcgacgg atcgcccgcc cgcttgcgca ggcgctccac gactcgcccg
     1021 tagtcgaagc ccgaggtgaa catccagcca ccgcgccgcg ggatcggcgt cgcctcatcg
     1081 gaagggaaga gcccgtacgg aaagagtcgc ccgccgacgg gcttgacgtt gcccaggcgg
     1141 ccggcatcac tgaacgtcgc cccgtccatc cacggcgcca gaggtgtgac ctgcagccag
     1201 gaaccgtcgc catacgttcg gaccagcgtc agggcgacct cgatccagag gaagtcaccc
     1261 atcttgtcca gccggtcgac cgcgcccggg atgcccgcgg tcggcagctc gacatcggcc
     1321 gcgtagcggc agtcggcgtc gtcgcaggga ggaagctcga cggtcgcgag ggcatcgatg
     1381 gacaccccga gacccctgtc gccagaaccc ccgaaggcct gcatcccggc gaacagcacg
     1441 gccggacccg actggaccgg gaggccctgg aggaccggat cgatctcaac gtggagcctg
     1501 aggccgtcgc gggtcagcct gccttcggtc actgaccagg tccctggccc gccctggcgc
     1561 ccgtcgttgc gcgcctcgtc gaacaggacg tcgccgccga tgtggaccgg aacccgggca
     1621 aggccgcggt cggcccgggc agcgggtggc gccgcggcga gggcaagcgc aacgagcgcc
     1681 acgagcaagg cgagaggccg gagacgggcg accatccccg cttcgacgtc gcgcggggcc
     1741 gtggtgctgc gcgaggtcga ggccgctaga cccgttcgaa cgccatcgcg acggagccgc
     1801 cgccgccgag gcaaagcgtg gccatgccgt atcggccgcc gcgccgggcg agctcgtgga
     1861 gcagcgtggc cacgatccgt gccccggacg ccccgatcgg gtggcccagg gcgatcgccc
     1921 cgccgttcac gttgaccttc gaccagtcga agcccagctc tcgtccgtcg gccagcgcct
     1981 gcgccgcgaa cgcttcgttg atctcgacca ggtcgtaggc ctcgatcggc aggccggtgc
     2041 gcgcctccag gccccggaca ccctggacgg gtgcgaggaa cagccacttg ggcgcgacct
     2101 ccgcctgcgc atagccgacg atccgtgcga gcggcgtcag gccgtgctct tccaccgccc
     2161 gctcggacgc cacgaccgtc gctgcggccc cgtccgtgat cccgggcgcg ttgccggcgg
     2221 tgaccgtccc cgtcgtggcc gagccgcggt cctccccctc gggcaggtcg aacgccgggg
     2281 cgagccgcgc aagcgcctcg gccgtcgtgt cgcggcgcgg gctctcgtcg acggtgacca
     2341 gcgtctcccg gcccttcgca tcccgcacgg tcaccggcgc cagctccgcg tcgaaccggc
     2401 cttcatcgat ggccgcgatc gcgtgctggt ggctcgcgag ggcgaacgcg tcctggtcct
     2461 cgcgcgagac ctggtcgtgg atcgccacgc gctccgcatg ggtccccatg tggcagtcct
     2521 cgacactgca ccagagcccg tcgaggaccg tcgagtcctc gagcgtcccg ttgcccaggc
     2581 ggtagccgaa gcgagccttg cgcaggagga acggcgcctg gttcatcgac tccatgccgc
     2641 cggccacgta caggtcgccg tccc
//
LOCUS       DBNHXO010000533         5465 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1963486, whole genome shotgun sequence.
ACCESSION   DBNHXO010000533 DBNHXO010000000
VERSION     DBNHXO010000533.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1963486"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="AC162A_10800"
     CDS             complement(<1..1126)
                     /locus_tag="AC162A_10800"
                     /inference="COORDINATES: protein motif:HMM:NF018004.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HIR5011374.1"
                     /translation="MPDIRPFRALRFDPEVVGDLGRVVAPPYDVIGPDLHTALLARSP
                     RNAVRLDLPHEEPPEDPDERYRRVARTFAAWRSDGALRKDPRASLYVYEQVYRVPGTS
                     LERTQRGFFARLRLEPFGPDGGVLPHERTLAGPREDRYRLLRATGVNTSPVVVLYRDP
                     AASTRTDLAAIAAQTPVSDVTDDDGTRHRLWVVADDGPEGAVGRLAAAAGAGPVFIAD
                     GHHRYETALRYRDERRMTRSCEPDPPFDFIFALLLDVDDELTVLPTHRVVRGLGDDGL
                     AALLAGLDGLFEVMPAATDALVARYDAAGGLAGGEGQMGLVTRDGARQLVARRPAFET
                     LVSGGEALRRLDVSLLGATLEALAGIDAAAVAGGERIRYSK"
     gene            1339..3786
                     /locus_tag="AC162A_10805"
     CDS             1339..3786
                     /locus_tag="AC162A_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011375.1"
                     /translation="MTRPSLPIPGRIARLLAVFALLSSSLLVSATPVAAADGLKMDAR
                     ALMQGHVRAGSWFAIAVDLQNDGPTIVGELRISGGLESRTRFGTPVELATGSRKQYLL
                     YALPPSFGGNMTVELVSDGKVLAKAPVAVALHDQTQLVVGLVSENPARLVGEFDLLPS
                     QNGIAPVIVPLTAADLPERVQAWAPLDRLIWQDVDAASLSPGQLAALRTWVAGGGRLV
                     ILGGTAPADVLTGFPDDLLPYRPNGVVDVDPAVLQPLLGLAPANATTLTALAGDPGSG
                     RTLATSGDRVIAADMAFGSGSVTILGFDPATSWIAEGDAIDVPMWRRLLPPRTGGNVA
                     LADDQTIVSAVANLPSLALPPIGSLLVLLAGYIVLVGPVNYLVLRWLDRREWAWVTVP
                     VLIAVFTVGAFGIGSLLRGSDVIVNEVAIVRGAPGTDAAVAQSYLGIFSPSRATYQLR
                     VSGDALLATPMNGDMFGGGESELDVLQGDPSRVRDLAVGFGSMRTVRAESSTTGPKVS
                     ASLQLDGGRIRGTVTNQSDRTLIAPAIVLGSSAATLKDLAPGASAEVNLPILSSNFDG
                     RQLSDTVVGPYNWDGGQMSESQQRQLIRRSIIDQLSFDPMTGASGVLPGDSPTLLAWG
                     NDPVTQAELEGAQVRREANVLYQIPLAMTIRGRNTFAADLLRSSALDVSANFFNKDPW
                     NISFGIGEVTMAYQPLPFEGRLVPEQLVIAMGFGGDVTGLGGKHAILREQARCDPDAD
                     GCAPPQDGMPELEILDVRTGTWVQFEHLTPGQAFEVENAARWVDPSTGQVQVKFVNER
                     QDGIGFQFKVEITGTVE"
     gene            3783..4748
                     /locus_tag="AC162A_10810"
     CDS             3783..4748
                     /locus_tag="AC162A_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5011376.1"
                     /translation="MTGIVHASGLVKRYDRTLAVAGLDLDVAEGEIFGLVGPNGAGKT
                     TTLRILSTLLVPDSGDAEVGGFSVRRNPNDVRRVLGFMPDSFGVYDDMKVWEYLDFFA
                     RCYGIPADRRRRMIGDLLDLVDLGAKRDTYVQGLSRGMQQRLCLAHALVHDPRVLLLD
                     EPASGLDPRARVELRELLRELRTLGKTILVSSHILPELEELCTSVAIIDRGQVLAQGR
                     VSEIEQRLRVGAVYRVRVLGDDAAIAAAQAWFAQQHNVVSAQVLEDATIEIGFHGDDD
                     GAAALLSAAVRADLRVATFSRAASDLEELFLQVTAQDPIAAGVPA"
     gene            4745..>5465
                     /locus_tag="AC162A_10815"
     CDS             4745..>5465
                     /locus_tag="AC162A_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5011377.1"
                     /translation="MTTISAPPTRRLGALRDVGTGIAAVGVKELRGRMRGRRAFIVLT
                     VHLLIVAGFAWMIETLAERSYTGGFNGNSVSASAEIGRQLFSALIMLLTLIVLVLAPA
                     STAGAISLEREKQTLDLLTTTPISSLAIILGKLLSALSWVFLLLLASIPVTALVFTFG
                     GVGPEDLVRAYVILFATAIAFGSMGLFVSAFVKRTQAATVINLVLTIVLTAGATFIFA
                     FWSAMTANGMLPNQVVGRNDSI"
BASE COUNT          741 a         1991 c         1913 g          820 t
ORIGIN      
        1 acttcgagta ccggatgcgc tcgccgccgg cgaccgcggc agcgtcgatc ccggcgagcg
       61 cctcgagcgt cgccccgagg agggagacat cgagccgccg cagggcctcg ccacccgaga
      121 ccagcgtctc gaacgccggc cgccgcgcca ccagctgcct ggcgccgtcc cgggtcacga
      181 gtcccatctg tccctcgccg ccggcgaggc cgcccgcggc gtcgtagcgc gcgacgagcg
      241 catcggtcgc tgccggcatg acctcgaaca ggccgtcgag gcccgccagg agggcggcga
      301 gcccgtcgtc gccgagaccg cgcacgactc gatgggtcgg gagcacggtc agctcgtcgt
      361 cgacgtcgag gagcaacgcg aagatgaagt cgaacggggg gtccggctcg cacgagcggg
      421 tcatgcgccg ctcgtcccga tagcgcagcg ccgtctcgta gcggtggtgg ccatccgcga
      481 tgaacaccgg cccggcgccg gcggctgccg ccaggcgccc gactgcgccc tcgggcccgt
      541 cgtcggcgac cacccacaac cggtggcgcg tgccgtcgtc gtcggtgacg tcgctcaccg
      601 gcgtctgggc cgcgatcgcc gccaggtcgg tccgcgtcga cgcggccggg tcgcgataca
      661 ggaccaccac cggcgaggtg ttgacgccgg tcgcccggag cagccggtac cggtcttcgc
      721 gcggtcccgc gagcgtccgc tcgtggggca ggaccccgcc gtccggtccg aacggctcga
      781 ggcggaggcg ggcgaagaac ccgcgctggg tccgctccag gctcgtgccc ggcacgcgat
      841 agacctgttc gtacacgtac aggctcgcgc gcgggtcctt ccgcaacgcg ccgtcggacc
      901 gccaggccgc gaacgtgcgc gcgacgcggc gatagcgctc gtcgggatcc tccggcggct
      961 cctcgtgcgg caggtcgagc cggacggcgt tccgcgggct ccgcgcgagc agtgccgtgt
     1021 gcaggtcggg accgatcacg tcgtacggcg gagcgaccac gcggccaagg tcgccgacga
     1081 cctcgggatc gaaccggagc gcgcgaaacg gacggatgtc gggcacgagt tcctctgggc
     1141 gcggatgcgg gctgggcgcc ggcgctgggc gcacgaccgc tccgacgagc ccggggccga
     1201 agcatcgtac gatagccgcg gtcggacggc caccgagggg gccggcggcg gctgccgaat
     1261 gcccgccctg caacttttcc aggggctggg gcgtcgatgc tccgaccgat cgctcgatcc
     1321 cccggtccag gagccagctt gacccgtccg agcctgccca tcccgggccg aatcgcccgc
     1381 ctgctcgccg tcttcgcgtt gctctcgagc tcgttgctgg tttcggccac cccggtcgcc
     1441 gccgccgacg gcctcaagat ggacgcgcga gccctcatgc agggccacgt gcgcgcggga
     1501 tcgtggttcg cgatcgcggt cgacctgcag aatgatgggc cgaccatcgt cggcgagctg
     1561 cgaatctccg gcggcctcga atcgcggacc cgcttcggca caccggtgga gctcgcgacc
     1621 ggatcgcgca agcagtacct gctgtacgcc ctgccgcctt cgttcggcgg caacatgacg
     1681 gtcgagctgg tcagcgacgg gaaggtcctt gcgaaggcgc cggtcgcggt cgcgctccac
     1741 gaccagaccc agctcgtggt cggtctcgtg tcggagaacc cggcccggct cgtcggtgag
     1801 ttcgacctgc tgcccagtca gaacggcatc gccccagtca tcgtgcccct cacggccgcc
     1861 gatctccccg agcgcgtgca ggcgtgggcc cccctcgacc ggctgatctg gcaggacgtg
     1921 gacgcggcct cgctcagccc ggggcagctg gccgcgctgc gcacctgggt ggcgggtggc
     1981 ggacggctcg tgatcctggg cggcaccgcc ccggcggacg tcctgaccgg cttccccgat
     2041 gacctgcttc cctaccggcc caacggcgtc gtcgacgtcg atccggcggt gctgcagcca
     2101 ctcctcggcc tggcccctgc gaacgccacg acgctcacgg cactggccgg cgacccgggc
     2161 agcggacgca cgctcgccac gtctggggac cgggtgatcg cggcggacat ggcgttcggc
     2221 tcgggctcgg tgacgatcct cggtttcgac cccgcgacct cgtggatcgc cgaaggcgac
     2281 gcgatcgatg ttccgatgtg gcgccgcctc ctgccgccgc gaaccggcgg caacgtcgcg
     2341 ctcgcggacg accagacgat cgtcagcgcg gtcgcgaacc tgcccagcct cgcgctgccg
     2401 ccgatcgggt cgctgctcgt gctcctcgcg ggctacatcg tgctggtcgg tccggtgaac
     2461 tacctcgtcc tccgctggct cgacaggcgt gagtgggcct gggtgaccgt cccggtgctg
     2521 atcgccgtgt tcacggtcgg cgcgttcggg atcgggtcgc tgctgcgcgg atcggacgtg
     2581 atcgtcaacg aggtcgcgat cgtccgtggg gcgcccggaa ccgacgccgc ggtggcgcag
     2641 tcctacctcg gcatcttcag tccgtcgcgc gcgacgtacc agctccgcgt gtcgggcgat
     2701 gccctcctgg ccacgccgat gaacggcgac atgttcggcg gcggcgaatc cgagctcgac
     2761 gtgctccagg gcgacccgtc ccgcgtgcgc gacctcgccg tcggcttcgg atcgatgcgc
     2821 accgtgcgcg cggaatccag cacgacgggc cccaaggtgt cggcctcgct ccagctggac
     2881 ggcgggcgca tccgcggcac cgtgaccaac caatcggacc ggacgctcat cgcgccggcc
     2941 atcgtcctcg gctcgtcggc cgcgacgctg aaggacctcg cgccgggtgc gtcggccgag
     3001 gtcaacctcc cgatcctgtc gagcaacttc gacggccgcc agctctccga cacggtcgtc
     3061 ggtccctaca actgggacgg cgggcagatg agcgagagcc agcagcggca gctgatccgg
     3121 cgctcgatca tcgatcagct ctcgttcgac ccgatgaccg gcgccagcgg cgtgctcccg
     3181 ggcgactccc cgacgctcct cgcatggggc aacgatccgg tcacgcaggc cgagctcgag
     3241 ggagcccagg tccggcgcga ggccaacgtc ctgtaccaga tcccgctcgc catgacgatc
     3301 cgcggccgca acaccttcgc cgccgacctg ctgcgcagca gcgcacttga cgtcagcgca
     3361 aacttcttca acaaggatcc ctggaacatc tcgttcggga tcggcgaggt gacgatggcc
     3421 taccagccgc tgccgttcga gggccgcctc gtgccggagc agctggtcat cgcgatgggc
     3481 ttcggcggcg atgtcaccgg cctgggcggc aagcacgcga tccttcgcga gcaggcccga
     3541 tgcgaccccg acgccgatgg ctgcgcgcca ccgcaggacg gaatgccgga gctcgagatc
     3601 ctcgacgttc gcaccggcac gtgggtccag ttcgagcact tgacgcccgg ccaggccttc
     3661 gaggtggaga acgccgcccg ctgggtcgac ccctccaccg gccaggtgca ggtgaagttc
     3721 gtcaacgagc ggcaggacgg catcggcttc cagttcaagg tcgagatcac ggggacggtc
     3781 gagtgacggg catcgttcac gcgagcggcc tcgtcaagcg gtacgaccgc acgctcgccg
     3841 tggcgggcct cgacctcgac gtggccgagg gcgagatctt cggcctcgtg ggccccaatg
     3901 gagccggcaa gacgacgacg ctgcggatcc tctcgacgct gctcgtcccc gacagcgggg
     3961 acgccgaggt gggcggcttc tccgtccgcc ggaaccccaa cgacgtgcgg cgcgtcctcg
     4021 ggttcatgcc ggactcgttc ggggtgtacg acgacatgaa ggtctgggag tacctggact
     4081 tcttcgcccg ctgctacggc atcccggcgg accgccggcg gcggatgatc ggcgacctgc
     4141 tcgacctcgt cgacctcggg gccaagcgcg acacctacgt ccagggcctg tcgcggggca
     4201 tgcagcaacg cctctgcctg gcccacgcgc tggtgcacga tccccgcgtg ctgctcctcg
     4261 acgagccggc ctcgggcctc gacccacgcg cacgggtcga gctgcgcgag ctgctgcggg
     4321 agctgcggac gctgggcaag acgatcctcg tctcgtccca catcctcccc gagctcgagg
     4381 agctgtgcac ctcggtcgcg atcatcgacc ggggtcaggt cctggcccag ggccgcgtct
     4441 cggagatcga gcagcggttg cgcgtcggcg cggtctaccg ggtccgtgtc ctcggtgacg
     4501 acgcggccat cgcggccgcc caggcctggt tcgcccagca gcacaacgtc gtctccgctc
     4561 aggtgctgga ggacgcgacg atcgagatcg ggttccacgg cgacgacgac ggcgcggcgg
     4621 cgttgctgtc tgccgccgtg cgcgcggacc tgcgggtggc cacgttctcc cgggcggcga
     4681 gcgacctcga ggagctgttc ctccaggtca ccgcccagga tccgatcgcc gcaggggtgc
     4741 cggcgtgacg acgatcagcg ccccgccgac ccgacgcctc ggcgcgctgc gcgacgtcgg
     4801 cacgggcatc gctgccgtcg gcgtcaagga gctccgcggg cgtatgcgcg gccggcgggc
     4861 gttcatcgtc ctgacggtcc acctgttgat cgtcgcgggc ttcgcctgga tgatcgagac
     4921 gctggccgaa cgctcgtaca ccggtgggtt caacggcaac agcgtctcgg catcggccga
     4981 gatcgggcgg cagctgttct ccgccctgat catgctgctc acgctgatcg tgctcgtgct
     5041 cgcgccagcc tcgaccgccg gcgcgatcag cctcgagcgc gagaagcaga cgctcgacct
     5101 gctgaccacg acgccgatct cgtcgctcgc gatcatcctg ggcaagctgc tctccgcgct
     5161 gagctgggtg ttcctgctgc tgctcgcgtc gatcccggtc accgccctcg tgttcacgtt
     5221 cggcggcgtg ggaccggagg acctcgtgcg tgcctacgtg atcctgttcg cgaccgcgat
     5281 cgcgttcggg tcgatgggcc tgttcgtgtc cgccttcgtg aagcgcacgc aggcggccac
     5341 cgtcatcaac ctggtgctga cgatcgtgct caccgccggg gcgacgttca tcttcgcgtt
     5401 ctggtcggcg atgacggcca acgggatgct gcccaaccag gtggtcggcc ggaatgactc
     5461 catcg
//
LOCUS       DBNHXO010000534         5121 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2956989, whole genome shotgun sequence.
ACCESSION   DBNHXO010000534 DBNHXO010000000
VERSION     DBNHXO010000534.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2956989"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="AC162A_10820"
     CDS             complement(<1..335)
                     /locus_tag="AC162A_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011378.1"
                     /translation="MPAEWRDRLDRLTRRLAVVALTVGVAGLAIGTVNVAADWRATAA
                     PLDAAPVSLDAITLDSAAESDRIAALTSQLDDAAAQIAAMRTAIAAANGNVTGDADSA
                     SALQRQLDAA"
     gene            complement(335..1045)
                     /locus_tag="AC162A_10825"
     CDS             complement(335..1045)
                     /locus_tag="AC162A_10825"
                     /inference="COORDINATES: protein motif:HMM:NF013918.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HIR5011379.1"
                     /translation="MRVGAATLLAIGAIAALVALGVTDQVLPAADPRTASMRPWIAAR
                     AMGITAYLLLALEVALGLVLSHPRNTAEWRKTKQVFPWHEMVTVFTAAFLTLHVALLA
                     TDPYADVGVIGALVPGYSTYRPVAVGLGSIALYAFIFTAVTAKWIHLLPAGWWLKAHR
                     FAAVAFFLTWMHAVLAGTDGGALLPLYLFTGIPILAGVGHRWWTARVRPARRVPPTST
                     TKPLTERPTAALVATEEL"
     gene            complement(1175..2113)
                     /locus_tag="AC162A_10830"
     CDS             complement(1175..2113)
                     /locus_tag="AC162A_10830"
                     /inference="COORDINATES: protein motif:HMM:NF013465.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR5011380.1"
                     /translation="MYGYVQWLGRRAMLALAASALISACGSPASSGATRFPTGTVVPV
                     VATTTVFADLVQQVGGSRVDVRSLVPKGGEVHTFDPTPSDVQKITEAKVIVRNGLGLD
                     DWLTALAEDAGTSAPIVTLGEGLDGVTYLEGEGGAGSVNPHLWLDVAYASKYVDRIQA
                     ALTDVDPAGASEYAARASAYKAVLTALDADVRQRLDALPASNRVVVSFHDAFPYVAAA
                     YGLTVVGTIIDAPGQDPSAGQIADLVRAIRASGAKAIFAEAQFSDALVKAVASETGAT
                     VVSDLYDDTLGDAPVDTYVGMMTWNADRVVDALRSR"
     gene            complement(2187..2621)
                     /locus_tag="AC162A_10835"
     CDS             complement(2187..2621)
                     /locus_tag="AC162A_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR5011381.1"
                     /translation="MIAEESLIEALDRAGYRLTEPRRTVAGLVAARDAHFTAADLVAD
                     ARKRHLRIGRATVFRALDVFTDLGLVERVDLPDGDHAYVGCDPVHHHHAICTRCGRSL
                     DIEDHGLSEVLAEVGERLAFTVTSHRLELYGLCDACRAAHRT"
     gene            complement(2618..3475)
                     /locus_tag="AC162A_10840"
     CDS             complement(2618..3475)
                     /locus_tag="AC162A_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR5011382.1"
                     /translation="MDLILEPLTLGFFVRALVASAMVGLVCAVVGTYVVLRGIAFIGD
                     AIAHASFPGVVAAFLLAVPFYLGAAVAAVGTALAIGYVTKRAGIRQDTAIGVLFAGTF
                     ALGVFMFSAIQGYVADLFSFLFGNVLAISPTDLVALMALGLGVLIVIALLWKELLYAT
                     FDPLGAAASGIPVDRLEYLFLALVALTIVVSLQAVGIILVVAMLVTPAATAQMLTVRF
                     TRLMAVAAIVGISSAVLGLYLSYWLDVASGATIVLVQTGLFLVAMVFGPRGMLARRRA
                     VGVALEAMV"
     gene            complement(3475..4344)
                     /locus_tag="AC162A_10845"
     CDS             complement(3475..4344)
                     /locus_tag="AC162A_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIR5011383.1"
                     /translation="MTLQSAHSHDHALDAAHAHAHADVWLSDVTAGYGERVALEHVSV
                     AVEPGSLLAVVGPNGAGKSTLLKLMAGLLTPWSGRIEILGAPAGRESRRVAYVPQAEN
                     VDWAFPVTVEDVVMMGRFPRLGPVRRPGRDDRRAVVDALAQVGMEKHLRTQIGRLSGG
                     QRRRVFLARALAAEPDLFLLDEPVTGIDATTQELLMDLLEAQAKRGKTVVATTHDLAC
                     AAQRFQQVLAVNRTVVAYGPSNIVLDADVLASAYGGHLLVLEGRTVILDDAHHHDDAA
                     PGERHFHERDGNR"
     gene            4488..>5121
                     /locus_tag="AC162A_10850"
     CDS             4488..>5121
                     /locus_tag="AC162A_10850"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR5011384.1"
                     /translation="MRRPLSGKQQDPPVSRRDRRRADRRARYDAARDERRGRSSGGGS
                     SFLSGRYLTIGAVVVGVLLIGFLAVGQLGDKATDTFIDPAIEYPVALVDGATIGAKDA
                     PVTLEVYEDFQCPVCARYSLTVEPQLVTKYVLPGTLRIVHHDIAILGNRTPDDESKIA
                     AVGASCASDQNRYWDYSHWVYANQAGENSGGFAPERLTAIAQAAGVDVSGF"
BASE COUNT          813 a         1759 c         1841 g          708 t
ORIGIN      
        1 gcggcatcga gctggcgctg cagcgcgctc gcgctgtccg catcgccggt cacgttcccg
       61 ttggccgcgg caattgcggt gcgcatggcc gcgatctggg cggcggcatc gtcgagctgg
      121 ctcgtcaatg cggcgatgcg gtcgctctcc gcggccgaat cgagggtgat cgcgtcgagg
      181 cttacgggtg ccgcgtcgag cggcgcggcg gtcgcccgcc agtcggcggc gacgttgacg
      241 gtgccgatgg ccagccctgc cacgccgacc gtcagggcga cgacggccag ccgccgcgtg
      301 agccggtcaa gccggtcgcg ccattccgcg ggcatcacag ttcctccgtt gcgacgagcg
      361 ccgcggtcgg ccgctcggtc aggggcttgg tcgtggatgt gggcgggacg cggcgcgccg
      421 gtcggacccg cgcggtccac catcgatggc cgacgcccgc gaggatcggg atgcccgtga
      481 acaggtagag cggcaggagc gcgccgccgt cggtccccgc caggacggcg tgcatccagg
      541 tgaggaagaa ggcgacggcc gcgaagcggt gagctttgag ccaccaaccc gccggcagca
      601 ggtggatcca cttggcggtg acggccgtga agatgaacgc gtacagggcg atcgacccga
      661 gcccgacggc gaccggccgg taggtggaat agcccgggac gagcgcgccg atcaccccca
      721 cgtcggcgta cggatctgtg gcgagcaagg cgacgtggag cgtcaggaac gccgccgtga
      781 acacagtcac catctcgtgc cacggaaaca cctgcttggt cttgcgccac tcggccgtgt
      841 tgcgcgggtg gctgaggacg agcccgagcg ccacctcgag ggccaggagc aggtaggccg
      901 tgatccccat ggcgcgggcc gcgatccagg gacgcatcga ggcggtgcga gggtcggcgg
      961 ccggcaggac ctggtccgtc acgccgagcg cgacgagggc ggcgatggcg ccgatggcga
     1021 ggagtgttgc ggcgcctacc cgcatggccg ggcggtgtcg tgcatgtcga gcctccaggg
     1081 gatctgctca actgaggcac gatccgccgg ataggtttca cgccacatag ccggacgatc
     1141 gtccgggtca gggccctcga tcgtcccggg ccggtcagcg actgcgtagc gcgtcgacca
     1201 cgcggtccgc gttccaggtc atcatgccca cgtaggtgtc gaccggcgca tcgccgagcg
     1261 tgtcgtcgta gaggtccgac acgacggtcg ccccggtctc ggacgcgacg gccttgacga
     1321 gggcgtcgct gaattgggcc tctgcgaaga tcgccttggc cccactcgcc ctgatcgcgc
     1381 ggacgagatc cgcgatctgg cccgcgctcg ggtcctggcc tggcgcgtcg atgatcgtgc
     1441 ccacgacggt cagtccgtac gcggccgcga cgtacgggaa ggcgtcgtgg aacgagacaa
     1501 ccacccgatt cgacgccggg agggcgtcga gccgctgtcg gacatccgcg tcgagagcag
     1561 tgaggacggc cttgtatgcc gacgcgcgcg ccgcgtactc cgacgccccc gcgggatcga
     1621 cgtccgtcag cgccgcctgg atgcgatcga cgtacttcga cgcgtacgcc acatcgagcc
     1681 aaagatgggg gttgaccgag ccggcgccgc cctcgccctc gaggtacgtc actccatcga
     1741 gaccctcccc gagggtgacg atcggcgcgc tcgtacctgc gtcctcggcc agggcggtca
     1801 gccagtcgtc gagtccaagg ccgttgcgca cgatgacctt ggcttcagtg atcttctgca
     1861 cgtccgaggg cgttggatca aaggtatgca cctcgccgcc tttcgggacc aggctccgca
     1921 cgtcaacgcg cgagccgccg acctgctgga cgaggtcggc gaacacggtg gtggtcgcga
     1981 cgaccgggac aacggttccc gttgggaacc tcgtcgcgcc agagctggca gggctcccgc
     2041 acgccgagat caatgccgac gcggccaggg cgagcatggc gcggcgaccc agccattgaa
     2101 cgtatccgta catcgagcca gatcccttct tatcgagact gagtctcaca attggttacg
     2161 acgaagctcc cgatgaactg cgaaggtcag gttcggtggg cggccctgca tgcgtcgcac
     2221 aggccgtaca gctcgaggcg atgggacgtg acggtgaacg cgagccgctc cccaacctcg
     2281 gccaggacct cggacagccc gtggtcctcg atgtcgagcg agcggccgca gcgggtgcaa
     2341 atcgcgtgat ggtggtggac ggggtcgcag ccgacgtagg cgtgatcgcc gtcgggcagg
     2401 tcgacgcgct cgacgaggcc aaggtccgtg aacacgtcga gcgcacggaa gacggtcgcc
     2461 ctgccgatcc ggaggtgacg cttccgggcg tccgcgacga ggtctgcggc cgtgaagtgc
     2521 gcatcgcggg ccgcgacgag gccggcgacc gtgcgccgcg gctcggtcag tcgatagccc
     2581 gcccggtcga gtgcctcgat gagcgactcc tcggcgatca caccatcgcc tcgagcgcga
     2641 cgccgacggc acgccggcgg gcgagcatgc cccgcggccc gaacaccatc gcgaccagga
     2701 acaggccggt ctggacgagc acgatcgtgg cgccgctggc gacgtcgagc cagtaggaga
     2761 ggtacagccc gagcaccgcg ctggagatgc cgacgatcgc ggccacggcc atcagcctcg
     2821 tgaaccggac cgtgagcatc tgcgcggtcg cggccggggt caccagcatc gcgacgacga
     2881 ggatgatccc caccgcctgg aggctcacga cgatcgtcag cgcgaccagc gcgaggaaca
     2941 ggtactcgag ccggtccacc ggaatcccgg acgcggccgc ccccagcggg tcgaacgtgg
     3001 cgtacaggag ctccttccac agcagcgcga tcacgatgag cacgccgagg ccgagggcca
     3061 tcagcgccac gaggtcggtc gggctgatgg cgaggacatt gccgaacagg aagctgaaca
     3121 ggtccgccac gtagccctgg atggcgctga acatgaacac gccgagcgcg aaggtgcctg
     3181 cgaacagcac cccgatcgcc gtgtcctggc ggatgcccgc ccgcttggtc acgtagccga
     3241 tcgcgagcgc cgtgccgacc gcggccaccg ccgcgcccag gtagaacggg accgcgagca
     3301 ggaacgcggc gacgacgccc gggaacgacg cgtgggcgat cgcgtccccg atgaacgcga
     3361 tgccgcgcag cacgacgtac gttccgacca ccgcgcacac gaggccgacc atcgccgagg
     3421 cgacgagcgc gcggacgaag aagccgagcg tgagcggctc gaggatcagg tccatcagcg
     3481 gttcccgtcc cgctcgtgga agtgccgctc gccgggtgcc gcgtcgtcgt ggtgatgcgc
     3541 gtcgtcgagg atgaccgttc ggccctcgag cacgagcagg tggccgccgt aggcgctggc
     3601 gaggacgtcg gcgtcgagca cgatgttcga gggcccgtag gccacgaccg tgcgattcac
     3661 ggccagcacc tgctggaacc gctgcgccgc gcaggcgagg tcgtgggtgg tcgcgaccac
     3721 cgtcttgccg cgcttggcct gggcctcgag gaggtccatc aggagctcct gggtggtcgc
     3781 gtcgatcccc gtcaccggct cgtcgagcag gaacaggtcc ggctccgccg ccagcgcccg
     3841 ggccaggaac acgcgccttc gctggccccc ggacagccgc ccgatctgcg tccgcaggtg
     3901 cttctccatc ccgacctggg cgagcgcgtc gacgaccgcg cgccgatcat cgcggcccgg
     3961 ccggcgcacc ggcccgaggc gcgggaagcg gcccatcatc accacgtctt cgaccgtgac
     4021 ggggaacgcc cagtcgacgt tctccgcctg ggggacatag gcgacccgac gggactcacg
     4081 tccggcgggc gccccgagga tctcgatccg gccagaccac ggggtgagca ggccggccat
     4141 cagcttgagc agcgtgctct tgccggcgcc gttcggacca acgacggcga gcaggctccc
     4201 gggctcgacg gcgaccgaca cgtgctcgag cgcgacccgc tcgccatacc cggccgtgac
     4261 gtcgctcagc cacacgtcgg cgtgggcgtg ggcgtgggcg gcgtcgagcg cgtggtcgtg
     4321 gctatgggcc gattgaagcg tcatatgaga cacagtatca actaggccgg ccccgagggc
     4381 aagtcccccg tgtgtgacgt ctgccacggc gagaacatgc cgtgtggctc ggggttggtt
     4441 tcctggtccc gacctagaat ggcgccgcgt tcccatccgt cacgagcgtg aggcgtcccc
     4501 tgagcggcaa gcagcaagat cctcccgtct cgcgacgtga ccgcaggcgt gcggaccgca
     4561 gggcccgcta cgacgccgcg cgcgacgagc gccgcggccg gtcgagcggc ggcggttcgt
     4621 cgttcctgtc gggccggtac ctcacgatcg gggccgtggt cgtcggcgtg ctgctgatcg
     4681 ggttcctcgc ggttggccag ctgggcgaca aggcgaccga cacgttcatc gacccggcga
     4741 tcgagtaccc ggtggcgctc gtggacggcg ccacgatcgg cgcgaaggac gcgccggtga
     4801 cgctcgaggt ctacgaggac ttccagtgcc cggtgtgcgc gcgctactcg ctgacggtgg
     4861 agccccagct cgtgacgaag tacgtgcttc cgggcaccct gcgcatcgtc caccacgaca
     4921 tcgcgatcct gggcaatcgc acgcccgacg acgaatcgaa gatcgcggcc gtcggtgcga
     4981 gctgcgcgag cgaccagaat cgctactggg actactccca ctgggtctac gccaaccagg
     5041 cgggcgagaa ctccggcggc ttcgctccag agcggctcac ggccatcgcc caggcggccg
     5101 gcgtcgacgt ctcgggcttc c
//
LOCUS       DBNHXO010000535         1716 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1371167, whole genome shotgun sequence.
ACCESSION   DBNHXO010000535 DBNHXO010000000
VERSION     DBNHXO010000535.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1371167"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1696)
                     /gene="purH"
                     /locus_tag="AC162A_10855"
     CDS             complement(<1..1696)
                     /gene="purH"
                     /locus_tag="AC162A_10855"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR5011385.1"
                     /translation="MSGSGSNLRALVAVGARGALGGDIVVVFADRPCAALDWAAEQGI
                     ETVLVPGGEDATLADTLAALDLDAVVLAGYLRLVGPDVLRRFGGRILNVHPSLLPAFP
                     GLHAVRDALGAGVAVTGVTVHLVDATLDGGPIVAQEGVPVLPGDDEAALLERLHAVEH
                     RLLPSAVAALLAGALSVTLGARRATLDPAAFDAHAPTPRRALVSVSDKAGLAGFGAGL
                     VARGFELVSTGGTARALREAGLPVTDVAAVTGFPEMLDGRVKTLHPRVHAGLLADRRR
                     ADHREALAAAGIAPFELVVVNLYPFAEAARKPGLSLDELVEEIDIGGPSMVRAAAKNH
                     ASVAIVTSPARYDAVLGALDEHGEVPIGLRSALAIEAFAHTAAYDARIAAELPCRMHD
                     AGIALPSEPGLPGAEDPYPPVLTISMAKVETLRYGENPHQPAARYRRTDREPRSAEGP
                     FASGEPPLQGKALSYNNVLDASAAASLARLLRGPGVVIVKHTNPCGAAERATLHEAWD
                     AALAGDPVSAFGGVVAITGPVDRALAERLTSLFLEVVVAPAFDASALEVLATKPNLR"
BASE COUNT          234 a          621 c          673 g          188 t
ORIGIN      
        1 gccgcaggtt gggcttggtg gcgagcacct cgagggcgga cgcgtcgaac gcgggcgcga
       61 cgacgacctc gaggaacagc gatgtcagcc gctcggccag cgcgcgatcg accgggccgg
      121 tgatcgccac gaccccgccg aacgccgaca cggggtcgcc ggccagcgcg gcgtcccacg
      181 cctcgtgcag ggtcgcccgc tccgccgcgc cgcaggggtt ggtgtgcttg acgatcacga
      241 cgccaggacc gcgcagcagc cgggcgagcg acgccgcggc cgacgcatcg agcacgttgt
      301 tgtagctgag tgccttgccc tggagcggcg gttcgccgct cgcgaacggg ccttcggcgc
      361 tgcgcggctc gcggtcggtc cgccggtagc gcgctgccgg ctggtgggga ttctcgccgt
      421 agcgcagcgt ctcgaccttg gccatcgaga tcgtcagcac cggcggatag gggtcctccg
      481 cgcccggcag gcccggctcg gacgggagtg cgatgcccgc gtcatgcatc cggcagggca
      541 gctcggcggc gatccgggcg tcgtaggccg cggtatgggc gaaggcctcg atcgcgagcg
      601 ccgaccgcag gccgatcggg acctcgccgt gctcgtcgag ggcgccgagg accgcgtcat
      661 agcgggctgg ggacgtcacg atcgcgaccg acgcgtggtt cttggcggcg gcacgaacca
      721 tggacggccc accgatatcg atctcctcga ccagctcgtc cagcgatagg ccgggcttgc
      781 gggccgcctc ggcgaacggg tacaggttga cgacgacgag ctcgaacggg gcgatccccg
      841 cggcggcgag cgcctcccgg tggtccgcgc gccggcggtc cgcgagcagc ccggcgtgga
      901 cgcgcgggtg gagcgtcttg acacggccgt cgagcatctc ggggaagccg gtgacggcgg
      961 cgacgtcggt gaccggcaga ccggcctcgc gcagcgcgcg cgccgtcccg cccgtgctga
     1021 cgagctcgaa gccgcgggcg acgaggccgg cgccgaagcc ggccagaccc gccttgtcgg
     1081 acacggacac cagggcccgc ctcggcgtcg gggcgtgggc gtcgaacgcg gcgggatcga
     1141 gggtggcgcg tcgggcgccg agcgtcaccg agagcgcccc ggcgaggagg gcagccacgg
     1201 ccgacgggag aagccgatgc tcgacggcgt ggagccgctc gaggagcgcc gcctcgtcat
     1261 cgcccgggag cacggggacg ccctcctggg cgacgatcgg cccgccgtcg agtgtcgcgt
     1321 ccacgaggtg gacggtgacg ccggtgacgg cgacgccggc gcccagcgcg tcgcgcacgg
     1381 cgtggagccc cggaaaggcc ggcagcaacg aggggtggac gttgaggatc cggccgccga
     1441 accgtcgcag gacgtcggga ccgacgagcc gcaggtagcc ggcgagcacg accgcgtcca
     1501 ggtcgagggc ggcgagcgtg tccgcgaggg tcgcgtcctc accgcccggc acgagcacgg
     1561 tctcgatccc ctgctccgcc gcccagtcca gggccgcgca cggccggtcg gcgaacacca
     1621 ccacgatgtc cccgcccagc gcgccccggg cgccgacggc gaccagcgcc cgcaggttgg
     1681 acccgctgcc cgacacgccg accgcgatcc gcggcg
//
LOCUS       DBNHXO010000536         1752 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1766982, whole genome shotgun sequence.
ACCESSION   DBNHXO010000536 DBNHXO010000000
VERSION     DBNHXO010000536.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1766982"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /locus_tag="AC162A_10860"
     CDS             complement(<1..1617)
                     /locus_tag="AC162A_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011386.1"
                     /translation="MQGSAGTSTGNDISFSIKGADSVTLRVLADASAGLDAGLFRKGT
                     GVTLTGVIGQRASRKGALDGYRLWLRDRGDVVVTTTPAPTPSPTPRGGPTPTPKPSAG
                     VPKPKVTSIRSALRQQGQRVTVEGVLTVNTTLLDASGRRTILEDGTAAIEIYLAAPDA
                     SLRLGARIRVTGTVGVAWGAPRLRAEETRVLGTRQPAVHALRSAPTAAVEWRLVRVSG
                     TIVEVHRSGDRWTADLQMRGARVLISGLAGSGIASTDLTEGRAATVTGIVKRPYPTAT
                     DRRFALVPRQRSDIVLGKVAAGPGASASATGASTGSSAGTPGPGASGVTGASAAGSPT
                     AATDIDLRDLDAHVGERVRVGGLVTTVQVDGVRLDDGTSEALLVFDGDAPELLGLLQV
                     GDALNATGVPEQRDGPVLVVAGAADVELVGELGAADPFSSGVTHAAAVDPGDWPEPTL
                     APVRAVLAPGLGFDSLATGLGMLVLVSLASVAVTLARRERSRRVLRSRIVARLEAFGR
                     GGEGSGSAPDRSAEPPLGAQTGPELGGNVRG"
BASE COUNT          224 a          666 c          632 g          230 t
ORIGIN      
        1 tccacgcacg tttccgccga gttccggccc ggtttgcgcc cccaacgggg gctcggctga
       61 ccgatcgggg gccgatccgg acccttcgcc gccccgcccg aaggcctcca gacgggccac
      121 gatgcgcgac cgcaggaccc gccgtgagcg ctcgcggcgg gccagcgtca ccgcgaccga
      181 tgcgaggctg acgagcacga gcatgcccag cccggtcgcg agggagtcga agcccagtcc
      241 gggcgcgagg accgcgcgaa ctggcgcgag cgtcggttcc ggccagtcac cggggtccac
      301 cgcggccgca tgggtcactc ccgagctgaa ggggtcggcg gcgccgagtt cgcccacgag
      361 ctcgacatct gccgcgccgg caaccacgag cacgggtccg tcgcgctgtt ccggcacgcc
      421 ggtcgcgttc agggcatcgc ccacctggag caggccgagc agctccggag cgtcaccgtc
      481 gaagacgagc agcgcctccg acgtcccgtc atcgaggcgg acgccgtcca cctgcacggt
      541 cgtcaccagg ccgcccaccc ggacgcgctc gccgacgtgg gcgtcgaggt cgcgcaggtc
      601 gatgtcagtc gcggcggtcg gggagcccgc cgcggatgcg ccggtcacgc ccgatgcgcc
      661 cggtcccggg gtgcctgcgg aactccctgt cgacgcgcct gttgctgatg ccgaagcgcc
      721 cgggccggcc gcgaccttgc cgagcacaat gtccgagcgc tgcctgggta cgagcgcgaa
      781 ccgccggtcc gtggccgtcg ggtacgggcg cttcacgatg ccggtgaccg tcgccgcccg
      841 accctccgtg aggtcggtgg acgcgatccc gctccccgcc agtccgctga tcaggacgcg
      901 cgcgccgcgc atctgcaggt cggcagtcca gcggtcgcca ctccgatgca cctcgacgat
      961 cgtgccgctc acgcgcacca gccgccattc gaccgccgcc gtcggcgcgg accgcagggc
     1021 gtgaacagcc ggctgccgcg tgcccaggac gcgcgtctcc tccgcccgga ggcggggagc
     1081 gccccaggcg acgccgacgg taccggtcac ccggatccga gcgccgagac gcagtgatgc
     1141 atcgggcgcc gccaggtaga tctcgatggc ggccgtgccg tcctcgagga tcgtccgcct
     1201 cccactcgca tcgaggagcg tggtgttcac ggtcagcacg ccctcgacgg tcacccgctg
     1261 cccctgctgg cggagcgcgc tgcggatgga cgtcaccttg ggcttcggga cgccggcact
     1321 cggcttcggc gtgggcgtgg ggccgccgcg gggcgtcggc gacggggttg gtgcaggcgt
     1381 ggtggtgaca acgacgtcac cgcggtcgcg gagccacagg cgatagccgt cgagcgcgcc
     1441 cttgcgcgag gcgcgctggc cgatgacgcc ggtcagcgtc acgcccgtgc ccttgcggaa
     1501 cagccccgcg tcgagtccgg ctgatgcgtc ggcgaggacg cgcagcgtga cgctgtcggc
     1561 gcccttgatc gagaacgaga tgtcgttgcc ggtcgatgtg cccgcggacc cctgcaccgt
     1621 ccccgcgacg cgtccgatgc gcccctcgtt cgcctcgccg accgccccgg ccttcagcgt
     1681 gatgggtccc gggaccgtcc cggaaccgat caccgtgacg ccgccggtcg gcggtcggag
     1741 ctcggtctgg cc
//
LOCUS       DBNHXO010000537         2012 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1174206, whole genome shotgun sequence.
ACCESSION   DBNHXO010000537 DBNHXO010000000
VERSION     DBNHXO010000537.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1174206"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="AC162A_10865"
     CDS             <1..756
                     /locus_tag="AC162A_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5011387.1"
                     /translation="VPWILWRPWAEGELDENRRLLQALNCLMVETTAGRVLIETGIGE
                     RLDDKARGMREYTGRPVVPAMLAAGFDPATVDVVAMSHLHFDHAGGLLLADGSRAFPR
                     ARIVAQRAEWEVALGGNSRLVASYDQPELRLVADWGAEGWADGDRELLPGVSVVRTGG
                     HSAGHQAIVVRGSGPGARTLAFFGDLCMRPWAANPRWVTAFDDFPLDSVEVKGELFRR
                     AVADDWLVALSHERRDPVGRLVPDRDRFRFEAL"
     gene            756..1298
                     /locus_tag="AC162A_10870"
     CDS             756..1298
                     /locus_tag="AC162A_10870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR5011388.1"
                     /translation="MADDPREILRPLRRVRQVRQFTAQPPSDAALDAMADVGRWSGSS
                     RNEQPWRFLILRDQAVLRRIWEIGVPQTRALETATAAIAIVLPERGHEIADAYDDGRA
                     AERILIAASFLGLGAAIQWVRNDVRQAVSEILGLPPDRYVRTIVALGHTTDAAARPKA
                     APGAARLPRDESVFRDRWPR"
     gene            complement(1321..1695)
                     /locus_tag="AC162A_10875"
     CDS             complement(1321..1695)
                     /locus_tag="AC162A_10875"
                     /inference="COORDINATES: protein motif:HMM:NF015735.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR5011389.1"
                     /translation="MRYVMLTFQGDEHLAAWESATPEERRLEIERTIAWFREHGALGR
                     IVGGEELGDGAQARTLRRGGFVTDGPFAETKEHLGGFIVLEVPDMATAIEVASGWPGL
                     DWDGDGVELRPAGDSASDPSMA"
     gene            complement(1801..>2012)
                     /locus_tag="AC162A_10880"
     CDS             complement(1801..>2012)
                     /locus_tag="AC162A_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR5011390.1"
                     /translation="DVRAYAERYELGYEIAFDASADIFNLYKVYALPTQFFIDPEGRI
                     LEVVNGPLTSEGAKARMDAWLPKDT"
BASE COUNT          257 a          714 c          760 g          281 t
ORIGIN      
        1 gtgccctgga tcctgtggcg gccatgggcg gagggcgagc tcgacgagaa ccgccgcctg
       61 ctccaggcgc tcaactgcct catggtcgag acgaccgcgg ggcgggtgct gatcgagacc
      121 gggatcgggg agcggctcga cgacaaggcc cggggaatgc gcgagtacac gggcaggccg
      181 gtggtcccgg cgatgctggc ggcgggcttc gatcccgcga cggtcgatgt cgttgcgatg
      241 agccacctcc atttcgacca cgcgggcggc ctgctgctcg cggacggctc gcgtgcgttc
      301 ccgcgggcgc ggatcgtggc gcagcgcgcc gagtgggagg tcgcgctcgg gggcaacagc
      361 cggctggtcg cgtcgtacga ccagcccgag ctgcggctag tggcggactg gggcgccgag
      421 ggctgggcgg atggcgaccg cgagctgctc ccgggcgtgt cggtcgtgcg gaccggcggt
      481 cactcggcgg ggcaccaggc gatcgtcgtg cggggttcgg gaccgggcgc gcggaccctg
      541 gccttcttcg gcgacctgtg catgcggccg tgggccgcca acccgcgctg ggtgacggcc
      601 ttcgacgact tcccgctcga cagcgtcgag gtcaagggcg agctgttccg gcgggcggtc
      661 gcggacgact ggctggtggc gctctcgcac gaacgccgcg acccggtggg ccggctggtg
      721 ccggaccgcg accggttccg cttcgaggcc ctgtgatggc ggacgacccc cgggagatcc
      781 tccggccgct gcgccgcgtc cggcaggtgc gccagttcac cgcccagccc ccgtccgacg
      841 ccgcgctcga tgcgatggcc gacgtcggcc gctggagcgg cagcagccgc aacgagcagc
      901 cgtggcggtt cctgatcctg cgcgaccagg cggtgctccg gaggatctgg gagatcggcg
      961 tcccccagac ccgcgcgctc gagacggcga cggctgcgat cgcgatcgtg ctgcccgagc
     1021 gtgggcacga gatcgccgac gcctacgacg acggccgggc ggcggaacgg atcctgattg
     1081 ccgcctcgtt cctcggcctg ggcgcggcga tccagtgggt gcggaacgac gtgcgccagg
     1141 cggtgagcga gatcctgggc ttgccccccg accgctacgt ccggacgatc gtcgcgctcg
     1201 gccatacgac cgacgcggcc gcgcgcccga aggcggcacc cggcgcggcg cgcctgcctc
     1261 gcgacgagtc ggtgttccgg gaccgctggc cccgctgacg cgacgagcga gcccaggcgg
     1321 tcaggccatg ctcgggtcgc tcgcggagtc gcccgccggg cgcagctcga cgccgtcgcc
     1381 atcccagtcg agccccggcc agccggacgc gacctcgatc gcggtcgcca tgtcgggcac
     1441 ctcgagcacg atgaagccgc cgaggtgctc cttggtctcg gcgaacggcc cgtcggtgac
     1501 gaacccgccc cggcgcagcg tgcgcgcctg cgcgccgtcg cccagctcct caccgccgac
     1561 gatccggccc agggcgccgt gctcgcggaa ccaggcgatc gtccgctcga tctcgagccg
     1621 tcgctcctcg ggggtcgcgg attcccaggc agccaggtgc tcgtcgccct ggaaggtcag
     1681 catcacgtaa cgcacagaac gtctcctcga tgacgcggcc atcatggggc cgccggaggg
     1741 tgaacgagcg gccgcctcct gatcgacgag cgccggcccg gttgcgccct gcgggacggg
     1801 ctaggtgtcc ttgggcagcc aggcgtccat gcgcgccttc gccccctcgc tggtgagcgg
     1861 cccgttcacg acctcgagga tccgcccttc ggggtcgatg aagaactgcg tcggcagggc
     1921 gtagaccttg tacaggttga agatgtcggc ggacgcgtcg aacgcgatct cgtagcccag
     1981 ctcgtagcgc tcggcgtagg cgcgtacatc gt
//
LOCUS       DBNHXO010000538         2864 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2957919, whole genome shotgun sequence.
ACCESSION   DBNHXO010000538 DBNHXO010000000
VERSION     DBNHXO010000538.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2957919"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="AC162A_10885"
     CDS             complement(<1..553)
                     /locus_tag="AC162A_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TolB-like translocation protein"
                     /protein_id="HIR5011391.1"
                     /translation="MTKTPRDPVRPAPAKRPARDPYGLLPSGVPIAAVVSILGLLVIG
                     FVTLGLTQGQLPTPGGADPGGAQASDEIPARTPTPSNIVIVPTPEPGIEVAGTLVYAK
                     DGNIWVQTGDTATQLTAPGKDVEDSMPSFSEDGKSVYFVRSRRADGKWSIDGEVKDYR
                     LNVPALMRVNVADGNTERLLDGLV"
     gene            complement(550..2442)
                     /gene="recJ"
                     /locus_tag="AC162A_10890"
     CDS             complement(550..2442)
                     /gene="recJ"
                     /locus_tag="AC162A_10890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR5011392.1"
                     /translation="MIDARHRWVFPEPLHLDPALRSAARALGLGTFAATVLARRGIAD
                     ADAMARFLGPADDGLNDPRLLPDAGVVITRVARAIEQQERVMVFGDFDADGLTGLAQL
                     VLTFRRLGLETLPYVPSRLEEGHGLSLAAVEAAARGGVALIVTVDTGSTSVAEIADAA
                     ARGIDVIVTDHHHLPEVLPAAVAIVNPQRADSAYPDARLSGSGVAFTVARLLLGELAG
                     AEAEARELADLATIGTVSDVSPILGENRSIARLGLELMRTAPRPGIAALLERAGTRAA
                     VVDLETVGFVIAPRLNAAGRVGEALDAARLLLAASAEEAATLAVVLEEANVTRRDLMR
                     TAIAEARAAFGLPDPGQAPGQQAWLDAPEPSESAIRVAGGPEAAAMLLTGPWPVGILG
                     LVAGRLADEANRPAVVGTVIATPDGDVVRASCRSDGRLSLAVILDACGDLLLRHGGHA
                     AAAGFELPAERWDAFTARFLAEAASAAPEDPRKPLNVDLALPASFVDYGLYRDLARLA
                     PCGTGNPEPLVAVLGLTVQRVRAANGGHTQLVLRRERDVLDGIAFGRPDLAEAVVEGD
                     RVDVVARLASRTFGGLETLQLEVRDVSTSGAHPRAAEVLARAAGNADATAVPVEIPVS
                     VGRGAA"
     gene            complement(2567..>2864)
                     /locus_tag="AC162A_10895"
     CDS             complement(2567..>2864)
                     /locus_tag="AC162A_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR5011393.1"
                     /translation="NKARHVGESFDRIVNHSILQTLGRSITTSLTVVITLLTLLLFAG
                     DAIGTFVLALLLGIVSGTYSSIFNAAPLLVVWHNRDDKRRQSELAARPSRRAAT"
BASE COUNT          394 a         1020 c         1094 g          356 t
ORIGIN      
        1 cgacgagccc gtcgagcagc cgctccgtgt tgccgtcggc gacgttgacg cgcatcagcg
       61 cgggcacgtt gagccggtag tccttgacct cgccgtcgat gctccacttg ccgtccgccc
      121 ggcgcgaccg gacgaagtac acggacttgc catcctcgga gaaggacggc atcgagtcct
      181 cgacatcctt gcccggcgcg gtcagctggg tagccgtatc gcctgtttgg acccagatgt
      241 tgccgtcctt cgcgtacacc agcgtgcccg ccacctcgat gccgggctcc ggggtcggga
      301 cgatgacgat gttcgacggg gtcggcgtgc gcgcggggat ctcgtcactg gcctgcgccc
      361 cgccggggtc tgcaccgccg ggcgtgggca gctggccctg ggtcagcccg agggtgacga
      421 agccgatcac cagcaggccg aggatcgaga cgaccgccgc gatcgggaca ccgctcggca
      481 gcaggccata cgggtccctg gctggccgct tggccggcgc ggggcggacg gggtcgcggg
      541 gggtcttcgt catgcggctc ctctgccgac cgacacgggg atctcaacgg ggaccgcggt
      601 tgcgtccgcg ttccccgccg cgcgggcgag gacctcggct gcgcgcgggt gggcgccgga
      661 cgtcgagacg tcgcggacct cgagctggag ggtctcgagc ccgccgaacg tgcggctggc
      721 caggcgggcg acgacgtcga cacgatcgcc ctccacgacg gcctcggcga gatcggggcg
      781 accgaatgcg attccatcga ggacgtcccg ctcgcgccgc agcacgagct gcgtgtggcc
      841 gccgttggcg gcccggacgc gctggaccgt caggccgagg acggcgacga gcggctccgg
      901 gttgccggtt ccgcacggcg ccaggcgcgc aaggtcgcgg tacaggccgt agtccacgaa
      961 cgaggcgggg agcgccaggt ccacgttgag cggcttccgg ggatcctccg gagccgcgga
     1021 cgcggcctcc gcgaggaagc gcgcggtgaa cgcgtcccag cgctccgccg gcagctcgaa
     1081 gccggcggcc gcggcgtgac cgccgtggcg caggagcagg tcgccgcacg cgtcgaggat
     1141 gacggcgagg ctgaggcgtc cgtcgctgcg acacgaggcg cggacgacgt cgccgtcggg
     1201 cgtcgcgatc accgtgccca ccacggcggg gcggttcgcc tcgtcggcca ggcggccggc
     1261 gacgagaccg aggatgccga ccggccacgg tccagtcagg agcatcgccg ccgcctccgg
     1321 gccaccggcg acccggatcg cggactcgga tggttccggc gcgtcgagcc atgcctgctg
     1381 gccaggcgcc tgccccggat ccggcagccc gaacgccgcg cgcgcctcgg cgatggcggt
     1441 gcgcatcagg tcgcggcgag tgacgttggc ctcctcgagc accacggcga gcgtggcggc
     1501 ctcctcggcc gacgccgcga gcaggaggcg cgccgcgtcg agcgcctccc ccacccggcc
     1561 ggcggcgttg agccgtggcg cgatgacgaa gccgacggtc tcgaggtcga cgacggcagc
     1621 ccgcgtaccg gcgcgctcga gcagcgcggc gatccccggg cgcggcgccg tgcgcatgag
     1681 ctcgagcccg aggcgcgcga tcgagcggtt ctcgcccagg atcggcgaca cgtcggacac
     1741 ggtcccgatg gtcgcgaggt cggcgagctc ccgcgcctcc gcctcggcgc ccgccagctc
     1801 gcccagcagc aggcgcgcga cggtgaacgc gacgcccgag ccggacaggc gcgcgtccgg
     1861 gtaggccgag tccgcccgct gcggattgac gatggcgacc gcggccggca gcacctctgg
     1921 caggtggtgg tggtcggtga cgatgacgtc gatcccgcgc gccgccgcgt ccgcgatctc
     1981 cgcgacgctg gtcgatccgg tgtcgactgt cacgatcagg gcgacgccac cgcgtgccgc
     2041 tgcctcgacg gccgccagcg agaggccgtg gccctcctcg aggcgggacg ggacgtacgg
     2101 cagcgtctcg aggccgagcc gccggaaggt cagcaccagc tgggccagcc cggtcaggcc
     2161 gtccgcgtcg aagtcgccga acaccatcac gcgttcctgc tgctcgatcg cgcgggcgac
     2221 ccgcgtgatc acgacgccag catccgggag cagccggggg tcgttgaggc cgtcgtcggc
     2281 cggcccgagg aagcgggcca tggcgtcggc gtcggcgatc ccccgccgcg ccaggacggt
     2341 ggccgcaaac gtcccgaggc cgagcgcccg ggccgccgac cgcaacgccg ggtcgaggtg
     2401 cagcggctcc ggaaacaccc agcggtgacg cgcgtcgatc atcgtccgga ggatgccacg
     2461 tccggcttac cccggactcg acgggacggc gaggcggtgc cggagctcgg gtccgtcagg
     2521 aacgcggacg ggagagtcgg gtcccagcct cggccgggtc ccgtcgtcac gtggcggcgc
     2581 ggcggcttgg gcgagccgcg agctcggatt ggcgccgctt gtcgtcgcgg ttgtgccaca
     2641 cgacgagcag cggggcggcg ttgaagatcg aggagtaggt cccggacacg atcccgagca
     2701 gcaacgcgag cacgaaggtc ccgatcgcgt cgcccgcgaa cagcagcagg gtcagcaggg
     2761 tgatcacgac ggtcaggctg gtggtgatcg accggcccag cgtctggagg atcgaatgat
     2821 tgacgatgcg atcgaacgac tcgcccacgt gccgcgcctt gttc
//
LOCUS       DBNHXO010000539         3302 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2759522, whole genome shotgun sequence.
ACCESSION   DBNHXO010000539 DBNHXO010000000
VERSION     DBNHXO010000539.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2759522"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="AC162A_10900"
     CDS             complement(<1..734)
                     /locus_tag="AC162A_10900"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5011394.1"
                     /translation="MDTASGPVVAERSSAPASTDGLRATDGLRAADVSLTFRDARTVQ
                     ALDAISLDVRPREVVALIGPNGCGKSTLLRVLSGLIAPDRGTVTLGGRPISGPDPRVG
                     LVFQEPRLLAWRSAVDNVRFPLELADWPRARQDARATELLGLVGLAETAQAKPSTLSG
                     GMRQRVSIARALALEPEVLLLDEPFSALDALTRERLNAELLDLWQRIGTTIVLVTHSI
                     SEAVFLADRVVVMSPRPGRIVADVPVG"
     regulatory      complement(814..982)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1025..1780)
                     /locus_tag="AC162A_10905"
     CDS             complement(1025..1780)
                     /locus_tag="AC162A_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5011395.1"
                     /translation="MADTAIERLAASRGRALRRMVAGQLEELREERGLSQREVAGAAG
                     VDRSWLARAEAGDANLTLHALAAIATVLGTEASVRLYPSSGPRLRDHIQVRLIETLLD
                     QLHPRWRPRLEVPVYRPVRGVIDLVLTDRAASELVAAEAHSEIRRAERQLRWAFEKAD
                     ALPSAVGWPWMDRHPLASRLLILRSTDATRAVVRAAPHVFGAAYPGRTAEAVAALTGA
                     AGTVPDAAIVWIDVLGSASRLLAAPPRGIDVGR"
     gene            complement(2002..>3302)
                     /locus_tag="AC162A_10910"
     CDS             complement(2002..>3302)
                     /locus_tag="AC162A_10910"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR5011396.1"
                     /translation="YPLDVGPDGRAEQSPADWWAGVSAAVRSIDVTGIDVIAVCGVGQ
                     GPTVAVVDGAGDPVRPAITWQDRRPTADGSDGGFGLLPTMAWLAREDRASVERATWLL
                     SAWDALGLWLSGEAATSLQGHETALDAVALRAAGVRPDAVPPPRPFGTVLGALRGAAA
                     EALGLRAGIPVVAGVNDGTASMLGAGLRVAGDAVDTGGASGGIGIYADAPVTVDGLFV
                     APAPLPGRWVVGGAMAALGASVDWLRESVLAGAASAEVLMAEAATVPPGAGGLLFLPY
                     LAGERAPLFDDAARGAFIGLTLAHGRAELARAVLEGAAFAMRSVAEPLAVAGAPIREL
                     RLAGRRSPGDAWARIKADVLGVPVAIPSVGESAVVGAAVLAAAGVGAVRDLEAGVAAM
                     TSIAARIEPDPDAHARYDSIYAVYRDLWPAIAPTVHALGR"
BASE COUNT          467 a         1268 c         1183 g          384 t
ORIGIN      
        1 ccgaccggca cgtcggccac gatgcgtccc gggcgcggcg acatgacgac gacgcggtcg
       61 gcgaggaaca ccgcctcgct gatgctgtgg gtcacgagca cgatcgtcgt gccgatgcgc
      121 tgccacaggt cgagcagctc cgcgttgagc cgctcacggg tcagcgcgtc gagggcgctg
      181 aacggctcat cgagcaggag cacctcgggt tcgagggcga gggcgcgggc gatcgacacc
      241 cgctggcgca tgccgcccga gagggtcgag ggcttcgcct gcgcggtctc ggcaaggccg
      301 acgaggccga ggagctcggt cgcccgggcg tcctggcgcg cgcgcggcca gtcggcgagc
      361 tcgagcggga agcggacgtt gtcgaccgcg gagcgccacg cgagcaggcg cggctcctgg
      421 aacacgaggc cgacccgtgg gtccggaccg ctgatcggcc gcccgccgag cgtgacggtg
      481 ccgcggtcgg gtgcgatcag ccccgagagc acgcgcagca gcgtgctctt gccgcagccg
      541 ttggggccga tgagggcgac gacctcgcgg ggccggacgt cgagggagat ggcgtcgagg
      601 gcctggaccg tacgggcgtc gcggaacgtg agcgagacgt cggcggcgcg gaggccgtcg
      661 gtggcgcgga ggccgtcggt ggaggcgggc gcggacgagc gctcggcgac caccggtccg
      721 ctcgctgtgt ccactccacg tcctccgtcg cacgtctcgg gcgcaacggg gcaggagtga
      781 cgccgacgga gtcccttccg ggacgtgtcg acccacggcc ttcttccatc cggactgtac
      841 cgtcggctcc ggccggctcg gctgacgcct cgcctcggat ctgcagtccc gcgaacatcg
      901 tccgcgtcgc cgctcgtggg ctcgtcccgc gtcgcgggac cctaccaccg gtcgggactc
      961 gcaccctgcc ccgaaggctc tatcccgatc ctaccaccgg atcgcggcgt gcctgctctg
     1021 ccgatcaccg cccgacgtcg atcccgcgag gcggagcggc tagcagccgg ctcgccgaac
     1081 ctaggacgtc gatccacacg atcgccgcgt cggggacggt ccctgccgcg cccgtgagcg
     1141 ccgcgaccgc ctcggccgtt cgaccgggat acgccgcacc gaacacgtgc ggcgccgcac
     1201 ggacgactgc gcgcgtcgcg tcggtcgacc gcaggatgag gagtcgcgag gcgaggggat
     1261 gccgatccat ccatggccag ccgaccgcgg acgggagggc gtcggccttc tcgaacgccc
     1321 atcggagctg gcgttccgcg cggcggatct cgctgtgcgc ctcggcggcc acgagctcgc
     1381 tcgccgcgcg atccgtgagg acgaggtcga ttacgccacg aacaggccga tagacaggca
     1441 cctcgaggcg gggacgccac cgcggatgca gctgatcgag cagtgtctcg atgagccgga
     1501 cctggatgtg gtcccgcagc ctgggaccgc tcgacggata cagccgcacc gaagcctcgg
     1561 tgcccagcac ggtcgcgatg gcggcaaggg cgtggagggt caggttcgcg tcgccggcct
     1621 ccgccctcgc cagccaggaa cggtcgacgc cggcggcccc ggcgacctcg cgctgggaga
     1681 gcccgcgctc ttcgcgcagc tcctcgagct gcccggcgac catgcggcgc agcgcacgtc
     1741 cgcgcgacgc ggccagtcgc tcgattgccg tgtcggccat ggtcgacctc ctgggcggcg
     1801 ggcatgtgcc tcagatcatc caaatcgctg catatctggc ccttactcgg ttgtggctgc
     1861 ctggttcacg ccacattcgt gcctgggagg cacggaagcg ctcgcagcgc ccagtctcga
     1921 gcgttggagg cttgccgacg gaggaaattg gcgggtttca agcacactcg gcgtgggcga
     1981 cggaggcgcg gagcgagccg atcagcgtcc gagcgcgtgc accgtggggg cgatggccgg
     2041 ccagaggtcg cggtagaccg cgtagatcga gtcgtagcgg gcgtgggcgt cgggatcggg
     2101 ctcgatgcgc gccgcgatcg aggtcatcgc cgcgacgccc gcctcgaggt cgcggaccgc
     2161 gcccacccca gccgcggcga ggaccgccgc ccccacgacc gccgactccc ccacggacgg
     2221 gatcgccacc ggcacgccca ggacgtccgc cttgatgcgc gcccaggcgt cgccgggcga
     2281 gcgcctccca gcgagacgga gctcgcggat cggggcgccg gcgacggcca ggggttcggc
     2341 cacggatcgc atcgcgaacg cggccccctc gaggactgcg cgggccagct cggcccgtcc
     2401 gtgcgccagg gtcagcccga tgaacgcgcc tcgtgccgca tcgtcgaaca gcggcgcccg
     2461 ctcgccggcc aggtacggca ggaacagcag tccgcccgca cccggcggca ccgtggccgc
     2521 ctcggccatc aggacctcgg cagacgcagc gccggcgagc accgactcgc gcagccagtc
     2581 gaccgaggca ccgagcgccg ccatcgcgcc ccccacgacc cagcgtcccg gcagcggcgc
     2641 cggggcgacg aacaggccat caacggtgac gggcgcatcc gcgtagatcc cgatcccgcc
     2701 cgacgccccg ccggtgtcca cggcgtcgcc cgcaacacgc agccccgccc ccagcatgct
     2761 cgcggtcccg tcgttcaccc cggccacgac ggggatcccg gcccgcaacc cgagcgcctc
     2821 ggcagccgcc ccgcgcaggg ccccgagcac cgtcccgaaa ggtcgcggcg gcgggacagc
     2881 gtccggccgc acacccgcgg cgcgcaaggc caccgcgtcc aacgccgtct cgtgcccctg
     2941 cagcgacgtc gcggcctccc ccgagagcca caggcccagc gcgtcccacg ccgacaggag
     3001 ccacgtcgcc cgctcaacgg acgctcggtc ctcgcgcgcc agccaggcca tcgtcggcag
     3061 cagcccgaac ccgccgtccg acccgtccgc cgtcggccgc cggtcctgcc atgtgatcgc
     3121 cggccgcacg gggtccccgg caccatcgac caccgccacc gtcgggccct gcccaacccc
     3181 gcacacggcg atgacgtcga tcccggtgac atcgatcgac cggaccgccg ccgagacccc
     3241 ggcccaccag tcggccggcg actgctccgc ccggccgtcg gggcccacgt ccagcggata
     3301 gc
//
LOCUS       DBNHXO010000540         2503 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3554641, whole genome shotgun sequence.
ACCESSION   DBNHXO010000540 DBNHXO010000000
VERSION     DBNHXO010000540.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3554641"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="AC162A_10915"
     CDS             complement(<1..512)
                     /locus_tag="AC162A_10915"
                     /inference="COORDINATES: protein motif:HMM:NF019544.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol phosphotransferase CPT family
                     protein"
                     /protein_id="HIR5011397.1"
                     /translation="MATGRVIVLNGTSSSGKSTLLRALQARLDGPWLGVGIDTVVFAL
                     PKRYLDQPLWSEVFRYVDAEPGADAPFRIETGELGHHLVEGMHRMIASLADAGLAVIV
                     DHVLLDPAWVVDLARRLAAHEVLFVGVRCPREVVVERERARRDRTIGQAAAQFDVVHR
                     AGGYDLEVDTS"
     gene            585..1265
                     /gene="folE"
                     /locus_tag="AC162A_10920"
     CDS             585..1265
                     /gene="folE"
                     /locus_tag="AC162A_10920"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIR5011398.1"
                     /translation="MSDSTTPRQLPDHVRELVEEIESEIGDDVGQPAHAASPNGRIES
                     AVREILTEIGEDPDREGLRTTPDRVHRMYSELTAGYHVDPERLINRAVFEVDYSEMVV
                     VRDVPFYSLCEHHLLPFFGNAHVAYIPDGRVIGLSKIPRIVEMYARRLQVQERLTQQV
                     ADFLMDRLQPQGVGVVIEATHLCAAMRGVRKPGTVMTTAAVLGLFRRNDKTRAEFFAH
                     LGRPAPGA"
     gene            1274..2167
                     /locus_tag="AC162A_10925"
     CDS             1274..2167
                     /locus_tag="AC162A_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR5011399.1"
                     /translation="MAHTHATPAAANRNRLAAVFFVTLGVFVFELIGGIAANSLALIA
                     DAGHLLTDVAGIGLTLVAIWFAGRPANLNRSYGLMRLEILAAVVNAMLLFAVAAYVLV
                     EAWRRWNAPPEVASGLMLVVAVIGLLANGVSLVVLHNAQRESLPMRGAYLEVMGDLVG
                     SVAVIVAAILITVTGQVRADAIASVFIGLLILPRTWSLLKEAVDVLLEASPKGIDLGE
                     VRSHILEAPGVADVHDLHAWTITSGVNVVSAHVVLEADASPPAVLDYLCGCLSGDFDI
                     EHSTFQLETSDRRRLETAHHA"
     gene            2395..>2503
                     /locus_tag="AC162A_10930"
     CDS             2395..>2503
                     /locus_tag="AC162A_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HIR5011400.1"
                     /translation="MTDRPLKVLIAKPGLDGHDRGAKVLARGLRDEGFEV"
BASE COUNT          363 a          912 c          856 g          372 t
ORIGIN      
        1 gacgtgtcga cctcgaggtc gtaaccgccg gcccggtgga cgacgtcgaa ttgcgcggcc
       61 gcctggccga tcgtccggtc gcgccgcgca cgctcccgtt cgacgacgac ctcacgcgga
      121 cagcggacgc cgacgaacag cacctcgtgg gccgccaggc ggcgcgccag gtcgacgacc
      181 caggccgggt ccagcagcac gtgatccacg atcaccgcca ggccggcatc ggcgagcgag
      241 gcgatcatcc ggtgcatccc ctcgaccagg tggtggccca gctcgccggt ctcgatccgg
      301 aacggcgcgt cggccccggg ctcggcgtcg acgtaccgga acacctcgct ccacaacggc
      361 tggtcgaggt accgcttggg cagggcgaac acgacggtgt cgatcccgac cccgagccac
      421 ggcccgtcga gccgcgcctg gagcgcccgc agcagtgttg acttgccgga gctcgacgtc
      481 ccgttgagca cgatcacgcg gccggtcgcc atccgctcat gatggcgagt tccggcccgc
      541 gtcgggtggc gggtcgtcgg ccgcgcgcta ccatcggcca acagatgagc gatagcacaa
      601 cgccccggca gctccccgac catgtccgcg agctcgtcga ggagatcgag agcgagatcg
      661 gcgacgacgt cggtcagccg gcgcacgcgg cgtcaccgaa cgggcggatc gagtccgcgg
      721 tgcgcgagat cctgaccgag atcggggagg accccgatcg cgagggcctg cggacgacgc
      781 ccgatcgcgt ccaccggatg tactcggagc tcacggccgg ctaccacgtg gaccccgagc
      841 ggctgatcaa ccgggccgtg ttcgaggtcg attactccga gatggtggtc gtccgcgacg
      901 tgccgttcta ctcgttgtgc gagcaccacc tgctgccgtt cttcggcaac gcgcacgtcg
      961 cctacatccc cgacgggcgg gtgatcgggc tgtcaaagat cccgcgcatc gtcgagatgt
     1021 acgcgcgccg gctccaggtc caggagcggc tgacccagca ggtcgccgac ttcctcatgg
     1081 accggctcca gccccagggc gtcggcgtcg tgatcgaggc cacgcacctg tgcgcggcca
     1141 tgcgcggcgt ccgcaagccc ggcacggtca tgacgaccgc cgcggtgctc ggcctgttcc
     1201 ggcgcaacga caagacgcgg gccgagttct tcgcccatct cggacggccg gcgccgggcg
     1261 cctgacgccg ccgatggccc acacccacgc cacgcccgcc gccgcgaacc ggaaccggct
     1321 cgcagcggtg ttcttcgtca cgctcggcgt gttcgtgttc gagctgatcg gcgggatcgc
     1381 cgccaacagc ctcgcgctca tcgccgacgc cggccacctg ctgaccgacg tcgccggcat
     1441 cgggctcacc ctggtcgcga tctggttcgc cggccgccca gccaacctca atcgctcgta
     1501 cggcctgatg cgcctcgaga tcctcgcggc cgtcgtcaac gcgatgctgc tgttcgcggt
     1561 ggccgcgtac gtgctcgtcg aggcctggcg gcggtggaac gcgccacccg aggtcgcgtc
     1621 ggggctgatg ctcgttgtcg ccgtgatcgg cctgctcgcg aacggcgtct cgctggtcgt
     1681 cctgcacaac gcgcagcgcg agagcctgcc gatgcggggt gcctacctcg aggtgatggg
     1741 cgacctcgtc ggctccgtgg ccgtcatcgt cgccgccatc ctgatcaccg tgacgggcca
     1801 ggtgcgggcg gacgcgatcg cctccgtgtt catcgggctg ctgattctgc cgcggacctg
     1861 gtcgctgctc aaggaggcgg tcgacgtcct gctcgaggcg tcgcccaagg ggatcgacct
     1921 gggcgaggtg cggtcgcaca tcctggaggc cccgggcgtc gccgacgtcc acgacctcca
     1981 cgcctggacg atcacctcgg gcgtgaacgt cgtctccgcg cacgtcgtcc tggaggccga
     2041 cgcgagcccg ccggcggtgc tcgactatct ctgcggttgc ctgtccggcg acttcgacat
     2101 cgagcactcg acgttccagc tggagacctc cgaccgccgc cggctcgaga ccgcccacca
     2161 tgcgtgagac ctcctgagat ccgcggcgtg cctggcaggc aacaagaccg tctcgaaggg
     2221 ccatcggatc tcgctccagc gggtccacgc ctccggatcg tgtctgtgag acacgaaacc
     2281 gggacctcgg aggcggtcag cggcacttcg acgtgaaagt gttgcctgac aggcacgctg
     2341 ccccgcggac ggcaaccggt cgttgcgcgc gtgccgcccg cctacaatcc gggcatgacc
     2401 gatcgaccgc tcaaggtgct gatcgccaag cccggcctcg acgggcacga ccgcggggcg
     2461 aaggtgctgg cgcgcggcct gcgcgacgag ggcttcgagg tcg
//
LOCUS       DBNHXO010000541         1663 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2960368, whole genome shotgun sequence.
ACCESSION   DBNHXO010000541 DBNHXO010000000
VERSION     DBNHXO010000541.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2960368"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="AC162A_10935"
     CDS             complement(<1..176)
                     /locus_tag="AC162A_10935"
                     /inference="COORDINATES: protein motif:HMM:NF017999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5011401.1"
                     /translation="MQFSGTVDINAPRDRVRAFLMDPNEVGPCGPGVESIEVIDEDHF
                     KAKAKVGIGFISAK"
     gene            complement(202..1251)
                     /locus_tag="AC162A_10940"
     CDS             complement(202..1251)
                     /locus_tag="AC162A_10940"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR5011402.1"
                     /translation="MPGRSLFPRVAELEASGGTFAVATVVAVQRPTSARPGAMGIVHP
                     DGTIEGWVGGSCAQPVVVREALRSLADGHPRLLRLSKTAPAEGRRGDGVVELVMTCHS
                     GGTLEIYVEPHLPAPSLWLAGTTPIVGALATLGAATGWRVSVFDPIADEDAFPGAERV
                     STMTALGRVDPELSPYVVVATQGIWDEEALAAALRRDVSYVGLVASPTRSGVVRAWLR
                     DEANVPEERLAALRAPAGIDLGAETPEEIALSILAELVQVRRGRASFVAVPGPATLAG
                     EGAAGALEAVSLAPVVDDIVLLDPVCGMTVDREHARHLAEHDGAVYAFCSMGCRTAFI
                     KEPGAYVAGSTTAGA"
     gene            complement(1256..>1663)
                     /locus_tag="AC162A_10945"
     CDS             complement(1256..>1663)
                     /locus_tag="AC162A_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012715912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR5011403.1"
                     /translation="QVHVRRFVAVDDCGNIINPMIVDGQIHGGLTMGLAPALYEEISY
                     DAQGNNLAGTFMDYLLPTAVETPSWETGKTITPSPHHPLGAKGVGESATVGAPPAIAN
                     AVVDALSHLGVEHIDIPITPQKVWAILHEKGIS"
BASE COUNT          240 a          598 c          606 g          219 t
ORIGIN      
        1 aacttggccg agatgaaccc gatgcccacc ttcgccttgg ccttgaagtg gtcctcgtcg
       61 atgacctcga tcgactcgac gcccggcccg catggcccga cctcgttggg gtccatgagg
      121 aaggcccgaa cgcggtcgcg cggcgcgttg atgtcgacgg tgccactgaa ctgcatcgat
      181 gtctccgaag aagggccgcg gtcaggcgcc tgcggtggtc gagccggcga cgtacgcgcc
      241 gggctccttg atgaacgcgg tccgacagcc catcgagcag aaggcgtaca cggcgccgtc
      301 gtgctcggcg aggtgccggg cgtgctcgcg gtccacggtc atgccgcaga cgggatcgag
      361 caggacgatg tcgtcgacga cgggcgcgag ggacacggcc tcgagcgcgc ccgcggcccc
      421 ctcccccgcc agcgtcgccg gacccggcac cgccacgaag gacgcgcggc cgcggcggac
      481 ctggacgagc tcggcgagga tcgagagcgc gatctcctcg ggggtctcgg caccgaggtc
      541 gatgcccgcg ggcgcacgca gcgccgccag ccgctcctcg ggcacgttgg cctcgtcgcg
      601 cagccaggcg cggacgacgc cggaccgcgt cggcgacgcc accagcccga cgtaggacac
      661 gtcgcggcgg agggcggcgg ccagcgcctc ctcgtcccag atgccttggg tcgccacgac
      721 gacatacggc gagagctccg gatcgacgcg cccgagcgcc gtcatcgtcg agacccgctc
      781 ggcgcccggg aaggcgtcct cgtcggcgat cgggtcgaac accgacaccc gccagcccgt
      841 cgcggccccc agggtcgcga gcgcgccaac gatcggcgtc gtgccggcca gccacagcga
      901 tggcgcagga aggtgcggct ccacgtagat ctccagcgtg ccccccgagt ggcaggtcat
      961 gaccagctcg acgaccccgt cgccgcggcg cccttccgcg ggcgccgtct tcgagaggcg
     1021 gagcagccgc gggtggccgt ccgcgagcga acggagcgcc tcgcggacga cgacgggctg
     1081 ggcgcagctg ccgccgaccc agccctcgat cgtgccgtcg ggatggacga tccccatcgc
     1141 cccgggccgg gccgacgtgg gacgctggac cgccacgacc gtggcgaccg cgaacgtccc
     1201 tccgctggcc tcgagctccg cgacgcgcgg gaacaggctc cgtcccggca tggggtcagg
     1261 agatgccctt ctcgtgcagg atggcccaca ccttctgggg cgtgatcggg atgtcgatgt
     1321 gctcgacgcc gaggtggctc agggcatcga cgacggcgtt ggcgatcgcc ggcggggcgc
     1381 cgacagtggc cgactcgccc acgcccttcg cgcccagcgg gtggtgcggc gagggggtga
     1441 tggtcttgcc cgtctcccag gagggagtct cgacggccgt cgggagcagg tagtccatga
     1501 acgtcccggc gaggttgttg ccctgcgcgt cgtacgagat ctcctcgtac agcgcggggg
     1561 cgaggcccat cgtcaggcca ccgtggatct gcccgtcaac gatcatcggg ttgatgatgt
     1621 tgccgcagtc gtcgacggcc acgaaccggc gcacgtgcac ctg
//
LOCUS       DBNHXO010000542         5339 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2167092, whole genome shotgun sequence.
ACCESSION   DBNHXO010000542 DBNHXO010000000
VERSION     DBNHXO010000542.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2167092"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1748)
                     /locus_tag="AC162A_10950"
     CDS             complement(<1..1748)
                     /locus_tag="AC162A_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011404.1"
                     /translation="MRWLWIALRRIGDDRGATIGFAALVLVTALIAALAPRVLASLAD
                     EAVRAEVRSAPAAARSMVMLQNRTFGLGPAEDPLAQVRAAGDELDTTVPGSIRALVDH
                     REIEVESGRYRVQKDTTDPAFVRLRIQEDASDHIRYTDGRAPTASVTVRDDVGPDQVD
                     DVPVYEAAISAETARRFGISLGETVPLVGDPGDQLIGRTNGDLYAFATITGIYEALDP
                     DEDYWLGDQQLIHPVIRALSLEVQLLDAALLVDDATHAALAQYASTASPGLRYSWRSF
                     LDPRRLSQRSLAALTTDFRRLLVEYPSANITPSTDTALRTGMAAILETHQRRWAAAEG
                     IVAVMAIGPALVALATLTLIAVLAARRRQVTMSLARSRGASGGQVVVPTVVEGLLIAV
                     PGALVAVVIAVALIPGDLLTPTLAGVAIVVAIAVSVVTSTVVSIVRSRGPGKRDGDRV
                     VGRVGARRLMLEGLIVAGAIGAALLLRQRGLSAVSSAGSVGGFDPLIAAVPVLVGIAA
                     GIIVVRLYPFVLGAVAAVARRGRGLVLVLAARRATEGGTSSAVLLVLLATATVAAFAA
                     TSLESLDRSADAAAWQA"
     gene            complement(1751..4996)
                     /locus_tag="AC162A_10955"
     CDS             complement(1751..4996)
                     /locus_tag="AC162A_10955"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR5011405.1"
                     /translation="MLRRSRADLPVVFASWALIASALALLAAGTLYSDAVTLAGLHRE
                     LRNAPPAERAIVVRTRILPDRIALADEAVLPELRRAIAPTGGEIAKVLHSSAYADAAA
                     DPETVTDLTTFGSFEGLDRHATLVDGRWAEAGHSPVEATLSQPAATALGVTTGDTIAL
                     ESRLDPGRMVDVAITGTWRADPADPYWLGDPLVLDGVETSGRFTTRGPLVVQEADLLG
                     DALSEPLDAQWRAIPAIDGFSPEMLDAMATDVGGVLGRVNAALPQSNQASVTTKLPDI
                     LASVDRSVLVAQSGILLLLVQFGVLAAYAVILVAALLLERRRTETALLRSRGAGTGHL
                     VSMAFGEALLVVVPAVLAAPWLAMLLVQAVRLNPAMEGVGLSAPLPGPSTFITAALGG
                     LVALLVLTIPTLASGVSIAGVRAAVGRQAGRTLPQRLGLDLALVVLAVIAMLQLRLYG
                     ATLTRNAGGTLGVDPLLVAAPAIGLLGGAVLAIRIVPRLAELGERAMARTRGLIGSLG
                     SRQVARRPLRYTRAALLLILAAALGTFASAHAATWTQSQADQAAWAVGADMRMSPGPR
                     SDIPPWTTGGALRAIAGVTAATPVVQAAVDIGSTIRGGTLVAVDGNAMADIVRLRDDA
                     TGDATVAGLREIGSRRPPVPGIALPAGTRRIALRYDSAFTPVEGFAPVPEGHPGLRAA
                     VVLVDGDGRVARIQSDHGPIGVGGAELVIPQATQAGGEATDPLHLIGLDVGLSISGLT
                     DAGVQGQVDLTSVAVSPDDEGDTWTPVDLASADGRWVRDQGGTLIPFDPGTGPVVRLP
                     VQDLFPFFEYGWEMTVLPPRDPAPIPTLVGQAFLDRTGAEVGDQLRASVFGVPVTLDL
                     AARVDAFPPLDQSKPFVVVDGLALDLARAGARGSIVDADTWLLSVDPDRAAEVAATLR
                     SAPIGALDVVETSAVTADLAGDPLGLGVIGILGLGSLAALVFASIGFLVSATVSISER
                     IGEFALLKALGLAPRQLLLWLSIENFLLLATGLILGTLLGLVLAFLVLPFATLTPTGA
                     PPVPAPVVVVPPEAAIPTAVLAAILVLATVLLAMRQLPAARTSAVLRARDE"
     gene            5137..>5339
                     /locus_tag="AC162A_10960"
     CDS             5137..>5339
                     /locus_tag="AC162A_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011406.1"
                     /translation="MTVAAVILAARPATALADADGTPGVRRLADVAWSGGATPVVVCS
                     FDPDGAVAAALANTEATLVDPVDP"
BASE COUNT          801 a         1972 c         1972 g          594 t
ORIGIN      
        1 gcctgccagg cggccgcgtc cgccgaccgg tccaggctct cgagcgatgt cgcggcgaac
       61 gcggcgacgg tcgccgtcgc gaggagcacg agcaggacgg ccgacgacgt cccgccttcg
      121 gtcgcccgac gcgccgccag gaccaggacg agaccgcgtc cgcggcgggc gacggcggcg
      181 acggccccca aaacgaacgg gtagagccgg acgacgatga tcccggccgc gatcccgacg
      241 aggaccggga cggccgcgat cagcgggtcg aaaccgccca cggaccccgc cgagctgacc
      301 gcggagagcc cgcgctgccg gagcagcagc gccgcgccga tcgccccggc cacgatcaac
      361 ccctcgagca tcaggcggcg cgcgccgacg cgcccgacga cgcgatcgcc gtcccgcttg
      421 cccggtccac gcgagcgaac gatcgacacc acggtcgacg tgacgacgct gaccgcgatc
      481 gcgaccacga tcgccacgcc ggccagcgtc ggcgtcagga ggtcgcccgg gatcagggcg
      541 acggcgatga ccacggccac caacgccccc gggaccgcga tcaggaggcc ctcgacgacc
      601 gtggggacca ccacctggcc gcccgaggct ccgcggctgc gcgcgagcga catggtcacc
      661 tgccgccggc gcgcggcgag cacggcgatc agcgtcaggg tcgcgagggc gaccagcgcc
      721 ggcccgatcg ccatcaccgc gacgattccc tcggccgcgg cccagcgccg ctggtgcgtc
      781 tcgaggatcg cggccatccc ggtccgcagc gccgtgtcgg tcgacggcgt gatgtttgcc
      841 gacgggtact cgacgagcag gcgccggaag tcggtggtca gggcggcgag cgaccgctgg
      901 ctgaggcgcc gcgggtcgag gaacgagcgc cacgagtagc gcaggcccgg tgacgccgtg
      961 ctcgcgtact gggcgagcgc ggcatgggtc gcgtcatcga ccagaagtgc ggcatcgagc
     1021 agctgcacct ccagcgacaa ggcccggatc accgggtgga tcagctgctg gtcgccgagc
     1081 cagtagtcct cgtcggggtc gagcgcctca tagatgccgg tgatcgtcgc gaaggcgtac
     1141 aggtcgccat tcgtgcggcc gatcagctgg tcgcccgggt caccgacgag cggcacggtc
     1201 tcgccgagcg agatcccgaa ccgccgcgcg gtctcggccg agatcgccgc ctcgtagacc
     1261 ggcacgtcgt cgacctggtc tggtccgacg tcgtcacgga cagtgacgct ggcggtcggc
     1321 gcgcggccgt cggtgtagcg gatatggtcg cttgcgtctt cctggatccg gagccggacg
     1381 aacgccgggt cggtcgtgtc cttctgcacc cgataccgac cgctctccac ctcgatctcc
     1441 cggtggtcca cgagcgcgcg gatcgagccg ggcaccgtcg tgtcgagctc gtcgccggcc
     1501 gcgcgcacct gcgcgagcgg gtcctccgca gggccgagcc cgaaggtccg gttctggagc
     1561 atcaccatgc tccgcgccgc ggccggcgcc gagcggacct cggcccgcac cgcctcgtcg
     1621 gcgaggctgg ccagcacgcg cggcgcgagc gcggcgatga gggccgtgac gagcacgagc
     1681 gcggcgaacc cgatcgtggc tccccggtcg tcgccgatcc ggcgcagcgc gatccacagc
     1741 cagcgcacgg ctactcgtcc ctcgcccgga ggacggcgct ggtgcgggcc gccggcagct
     1801 gccgcatggc cagcaggacg gtcgcgagga ccaggatcgc cgccagcacc gcagtcggga
     1861 tcgcggcctc gggcggcacg acgacgaccg gcgcggggac cggcggggca cccgtgggcg
     1921 tgagcgtcgc aaacggcagc accaggaacg cgaggacgag cccgagcagc gtcccgagaa
     1981 tgagccccgt ggcgagcagc aggaagttct cgatcgagag ccacagcagc agttggcgtg
     2041 gcgccaggcc gagtgccttg aggagcgcga actcgccgat ccgctcggag atcgacaccg
     2101 tcgcgctcac gaggaagccg atcgacgcga acacgagcgc cgcgagcgag ccgagaccga
     2161 ggatcccgat cacgcccagc ccgagcgggt cgccggcgag gtcggccgtg acggccgagg
     2221 tctcgaccac gtccagggcg ccgatcggcg ccgagcgcag cgtcgcggcg acctcggccg
     2281 cccggtccgg gtccacggac agcaaccacg tgtccgcgtc gacgatcgag cccctggcac
     2341 cggcccgcgc aaggtcgagc gccagcccgt ccacgaccac gaacggcttc gactggtcga
     2401 gcggcgggaa ggcatcgacg cgcgccgcca ggtcgagcgt caccggcacc ccgaacacgg
     2461 acgcgcgcag ctggtcgccg acctccgcgc cggtgcggtc gaggaacgcc tggccgacga
     2521 gcgtcgggat cggcgcgggg tcccgcggcg ggagcaccgt catctcccag ccgtactcga
     2581 agaacgggaa caggtcctga acgggcagcc ggaccacagg tcccgtgccc ggatcgaagg
     2641 ggatcagggt gccgccctgg tcccggaccc accgcccgtc ggccgatgcc aggtccaccg
     2701 gcgtccaggt gtcgccctcg tcgtccgggc tcaccgcgac gctcgtcaag tcgacctgtc
     2761 cctgcacgcc ggcgtccgtg agcccggaaa tggacagccc gacgtcgagg ccgatcaggt
     2821 ggagtgggtc ggtcgcctca ccgccggcct gggtcgcctg ggggatgacg agctcggcac
     2881 ccccgacgcc gatcgggccg tgatccgact ggatccgcgc gacacggcca tcgccgtcga
     2941 cgagcacgac tgctgctcgc aggcccgggt ggccctcggg aacgggcgcg aacccctcaa
     3001 caggcgtaaa ggccgagtcg tagcggagcg cgatccggcg ggttcccgca ggcagcgcga
     3061 tgccgggcac cggcgggcgg cggctcccga tctcgcgcag accggcgacg gtcgcgtcgc
     3121 cggtcgcatc gtcgcgcagg cggacgatgt cggccatggc gttgccgtcg accgcaacga
     3181 gggtgccacc gcggatcgtc gacccgatgt cgacggccgc ctgcacgacc ggcgtggcgg
     3241 ccgtgacgcc cgcgatcgcc cgcaacgcgc cgccggtcgt ccacggcgga atgtcgctcc
     3301 ggggaccggg cgacatccgc atgtcggccc cgaccgccca cgccgcctgg tccgcttggc
     3361 tctgggtcca ggtcgcggcg tgagccgatg cgaaggtccc gagggcggcg gcgaggatga
     3421 gcagcagcgc ggcccgcgtg tagcgcagcg gccggcgcgc gacctgtcgg ctcccgagcg
     3481 acccaatcag cccgcgcgtc ctcgccatcg cgcgctcgcc gagctccgcg aggcggggca
     3541 cgatccggat ggcgaggacc gccccgccga gcaggccgat cgcgggtgcg gcgaccagca
     3601 gcggatcgac ccccagggtg ccgccggcgt tccgcgtgag cgtcgccccg tacaggcgga
     3661 gctggagcat cgcgatcacc gccaggacga caagggcgag gtcgaggccc aggcgttgcg
     3721 ggagcgtccg gcccgcctgc cggccgacgg cggcgcgaac gcccgcgatg ctcacgcccg
     3781 acgcgagcgt cgggatcgtc aggacgagca gcgccacgag gcccccgagg gccgcggtga
     3841 tgaacgtcga tgggccgggc aggggcgcac tcaggccgac gccctccatc gcgggattga
     3901 ggcgcaccgc ctggacgagc agcatcgcga gccacggggc ggcgagcacg gcggggacga
     3961 cgaccagcaa cgcctctccg aacgccatcg agacgaggtg acccgtgccc gcgccgcgcg
     4021 accgcagcaa cgcggtctcc gtacgccgcc gctcgaggag cagcgccgcg accaggatga
     4081 cggcataggc ggcgagcacg ccgaactgga ccagcagcag caggatcccc gactgcgcga
     4141 cgaggacgga acggtcgacc gacgcgagga tgtcgggcag cttggtggtg accgaggcct
     4201 ggttcgactg cgggagggcg gcattgacgc gaccgagcac cccgcccacg tcggtcgcca
     4261 tcgcgtccag catctccggg ctgaagccgt cgatcgccgg gatcgctcgc cactgcgcgt
     4321 cgagcggctc gctcaacgcg tcgcccaaca ggtcggcctc ctgcacgacc agcggcccgc
     4381 gggtggtgaa tcgcccgctg gtctcgaccc cgtcgagcac cagcggatcg ccgagccagt
     4441 agggatccgc cggatcagcg cgccaggtac cggtgatggc gacatccacc atccggcccg
     4501 ggtccaggcg gctctccagg gcgatggtgt cgccggtggt caccccgagc gcggtggcgg
     4561 ccggctggga gagggtcgcc tccaccggcg agtgacccgc ctccgcccat cgcccgtcga
     4621 ccagcgtcgc gtgcctgtcg agcccctcga acgatccaaa cgtcgtgagg tccgtcaccg
     4681 tctctgggtc cgccgcggcg tccgcgtagg cggaggagtg caggaccttc gcgatctccc
     4741 cacccgtcgg ggcgatggcc cgccggagct ccgggagcac ggcttcatcc gcgagggcga
     4801 tccgatccgg gaggatccgc gtccgcacga cgatcgcgcg ctcggccggg ggcgcgttcc
     4861 gcaactcgcg atggagtccg gcgagcgtga ccgcgtccga gtacagggtt cccgccgcca
     4921 gcagtgccag ggcgctcgcg atcagggccc acgaggcgaa cacgacgggc aggtccgcac
     4981 ggctgcgtcg gagcacggcg gaccataggc cgagcggtgt gggcagcgga ccttctcccg
     5041 gaggggggcg cggcgggcgt cactcgacaa taacgcgcgt cgaccggaaa ggttcgcgac
     5101 gtcgctacac tgcgcgggca gacaaggagg gagcccgtga ccgtcgccgc cgtcatcctc
     5161 gccgcccgcc cggcgacggc ccttgccgac gccgacggca cgccgggcgt ccgcaggctc
     5221 gccgacgtcg cgtggtccgg tggggcgacg cccgtcgtcg tctgctcgtt cgatcccgac
     5281 ggggccgtcg cggcggcgct cgcgaacacc gaggcgacgc tcgtggaccc cgttgatcc
//
LOCUS       DBNHXO010000543         4987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4350765, whole genome shotgun sequence.
ACCESSION   DBNHXO010000543 DBNHXO010000000
VERSION     DBNHXO010000543.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4350765"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="AC162A_10965"
     CDS             <1..429
                     /locus_tag="AC162A_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR5011407.1"
                     /translation="ERDAYNWVPESSRRARGFPMWAALRQLGRTGVAEMIGRTSDHAQ
                     RFAAGLADAPGVRILNDVVLNQVLVRFDDPSGGVEAGNARTDAVIAAIQADGTLWLGG
                     TRWHDLRAMRISVSGWSTTEADVDRSIAVIRRVADGIRAG"
     gene            733..2067
                     /locus_tag="AC162A_10970"
     CDS             733..2067
                     /locus_tag="AC162A_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5011408.1"
                     /translation="MGISTSPGLTSEEIVSLSRSHTLFSWSVQNALDPIAIDHAEGVY
                     LYTPEGRRILDFNAQLMSVNIGHGDRRVIDAMHEQALKLQFVQPAFTTEIRARLGAKL
                     AEILPGDMNKVFFTLGGAEAIENAIKLARHYTGRTKLLARYRAYHGATFGAMTLTGDP
                     RRWANEPGLVGVVRYPDTHRWGEAEPRPVAESLQGLEDVIKYEGAGSIAAVFLETIVG
                     TNGILIPPDGYLQGVREICDRYQILMVCDEVMAGFGRTGRWFAVDHWDVVPDLMTMAK
                     GLTSSYLPLGAVAMRDTIAEAMEGRMFYGGLTYSSHPVSLAASLATIGVYEEDDLIGN
                     AARLDGVMRGHHERLAAKHPSVGAHRNLGLFGILELVRSHEPWTPMTPFNGTSDEMKA
                     VGKFLRDNGLYTMIGNNAIHTNPPLCITAEQLAEGFEIIDRALDIADQAVTG"
     gene            2102..3052
                     /locus_tag="AC162A_10975"
     CDS             2102..3052
                     /locus_tag="AC162A_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5011409.1"
                     /translation="MSVPAQNEIGQLERSLELELEAAQPRGPRTTLRQALIIAGAIVF
                     VLLAWEGVKFLGGTPWRAPGALPGSPVLWNPPFRWAFANDINLPHVWNIAIALGRPFQ
                     SGRDVSLFQYLVGAALYTWREALLGFLLGSLLGLGLATAFVHSRLAERAFVPYVIASQ
                     TIPIIALAPLIVFALGQGVLSVVVIATYLTFFPVTIAMLRGLRSFDPRALELMRSYGA
                     SKTGIYTKLRLPASMPYFFTALKIAATASIVGAIIAEDTGAIKEGLGRVITTFNQYYA
                     TGPEKLWAAIFIAGVLGIGFFLVIRAAEIIVLRGRPGIDA"
     gene            3049..3912
                     /locus_tag="AC162A_10980"
     CDS             3049..3912
                     /locus_tag="AC162A_10980"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019925862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5011410.1"
                     /translation="MTDGTVPAPAAPIVRIKGVDKVFTTREGASTVALQGIDLEIRPR
                     EFVSLIGPSGCGKSTLLRVIGDLVQPSRGEVVVNGKPAPRARLDRDYGMVFQAPVLFD
                     WRSVEDNVKLPLEILGKPKSWRTQRAKEMLDLVELGEFTRHMPYQLSGGMQQRVAIAR
                     ALSFEPRILLMDEPFGALDEMTRERMNDEVLRIWEQTGTTIVFVTHSIPEAVFLSSRV
                     VVMSARPGRITKVVDVDLPRPRTDEVRETEAYFSLITEVREALRAGGSDGADEDGSAS
                     SRRTQAEGAVG"
     gene            3909..4760
                     /locus_tag="AC162A_10985"
     CDS             3909..4760
                     /locus_tag="AC162A_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5011411.1"
                     /translation="MSAATGMVGPSSSDTGPGRAARAARVAREYLPAVLVAVGALIVW
                     EGLIRALQMRAFILPAPSAILAALAENWSGERFPLFRSAQSTIFEAIGGFVIGVTAGT
                     AVAFMTARWAGMRSVMLPAAVAAGAIPIIAFAPLFNTWFGLTNPLSKMMMAAVLSFFP
                     VMANVTRGLAAVDPSSLELMRSYAASEGTVLRKVRIPNALPYFFTALKLASTLSLIGA
                     IVAEYFGGSTSVLGRMIVQSASALRFDITWAAVSLAAVTGIVFYLVIALVERLVIPWH
                     SSARNEA"
BASE COUNT          729 a         1753 c         1737 g          768 t
ORIGIN      
        1 gagcgcgacg cctacaactg ggtgcccgag tcgtcgcgcc gcgcccgggg gttcccgatg
       61 tgggcggccc tgcgccagct cgggcggacc ggcgtcgcgg agatgatcgg ccggacctcg
      121 gaccatgcgc agcggttcgc cgcgggcctc gcggatgcgc cgggcgtgcg gatcctcaac
      181 gacgtcgtgc tcaaccaggt cctggtccgg ttcgacgacc cgtcgggcgg cgtcgaggca
      241 ggcaatgcgc ggaccgacgc cgtgatcgcg gcgatccagg ccgacgggac gctatggctg
      301 ggcggcactc gctggcatga cctgcgggcc atgcggatct cggtgtccgg atggtccacg
      361 accgaggcgg acgtggaccg gtcgatcgcg gtgatccgtc gcgttgcgga cgggatccgc
      421 gccgggtaac tgatcagcgt tgctccaggg cccggcgcaa tggaacgcga gaggttcact
      481 cgcactgcgc cagggccaag cagatgccga tctgaacccg tggcgttcca ctcggccgtc
      541 gatcgggcct gcggaacgcc tgcggttcca tattggggga gttggggccg cgacccgctg
      601 cagcgaacgt caggcgttcc gccgcgatcg cctcggccgt gtgctgtaat gaccgccacg
      661 gcacgacccg ccggctccca cgtcgcgcac aggcacggtc gggctccgga tcccgaccgg
      721 aacggaggat ccatgggcat cagcacctcg ccgggcctga cgagcgagga gatcgtctcg
      781 ctctcccggt cgcacaccct gttctcgtgg tccgtccaga acgccctcga tccgatcgcg
      841 atcgaccatg ccgaaggcgt gtacctgtac acgccggagg gccggcggat cctcgacttc
      901 aacgcgcagc tgatgtcggt caacatcggc cacggcgacc ggcgcgtgat cgacgcgatg
      961 cacgagcagg cgctcaagct gcagttcgtc cagcccgcgt tcaccacgga gatccgcgcc
     1021 cgactcggcg ccaagctcgc cgagatcctg cccggcgaca tgaacaaggt gttcttcacc
     1081 ctcggcggcg ccgaggcgat cgagaacgcg atcaagctcg cccgccacta cacgggccgg
     1141 accaagctgc tggcccggta ccgcgcctac cacggcgcga cgttcggggc gatgaccctc
     1201 accggcgacc cgcgtcgctg ggcgaacgaa cccggcctcg tgggcgtcgt ccgctacccg
     1261 gatacccacc gctggggaga ggcggagccg cgcccggtgg cggagtcgct ccagggcctg
     1321 gaggacgtga tcaagtacga gggcgcgggc tcgatcgccg cggtgttcct cgagacgatc
     1381 gtgggcacca acggcatcct catcccgccc gacggctatc tccagggcgt ccgcgagatc
     1441 tgcgaccgct accagatcct catggtctgc gacgaggtca tggcggggtt cgggcggacg
     1501 ggccgctggt ttgccgtcga tcactgggac gtcgtgccgg acctgatgac gatggccaag
     1561 ggcctcacct cgagctacct gcccctgggc gccgtcgcga tgcgcgacac gatcgccgag
     1621 gcaatggaag gccgcatgtt ctacggcggc ctcacctaca gcagccaccc ggtgagcctc
     1681 gcggcgtcgc tggcgacgat cggcgtgtac gaggaggacg atctcatcgg caacgccgca
     1741 cgcctcgacg gcgtgatgcg cggtcaccac gagcgcctcg cggccaagca cccgtcggtg
     1801 ggcgcgcacc gcaacctcgg cctgttcgga atcctcgagc tcgtccggtc gcacgagccg
     1861 tggacaccga tgacgccgtt caacggcacc agcgacgaga tgaaggcggt cggcaagttc
     1921 ctgcgcgaca acggcctgta cacgatgatc ggcaacaacg cgatccacac caatccgccc
     1981 ctgtgcatca ccgcggagca gctggcggag ggcttcgaga tcatcgaccg cgccctcgac
     2041 atcgccgacc aggccgtcac gggctgagca cgagccagcg agcgaggagg accaggcgcg
     2101 cgtgagcgtc ccggcccaga acgagatcgg tcagctggaa cgaagcctcg agctggagct
     2161 cgaggccgcc cagccgcgcg gtccgcggac gacgctgcgc caggcgttga tcatcgccgg
     2221 cgccatcgtc ttcgtcctgc tggcgtggga aggcgtgaag tttctgggtg gcacgccgtg
     2281 gcgagcgccc ggcgcactgc cgggctcgcc ggtcctgtgg aatccgccgt tccgctgggc
     2341 cttcgcgaac gacatcaacc tcccccacgt ctggaacatc gcgatcgcgc tgggccggcc
     2401 gttccagagc ggcagggacg tgagcctgtt ccagtacctg gtgggggcgg cgctctacac
     2461 ctggcgcgag gcgctgctcg ggttcctgct gggcagcctg ctcgggctcg ggctcgcgac
     2521 cgcgttcgtc cactcgcggc tcgcggagcg cgcgttcgtg ccctacgtca tcgccagcca
     2581 gaccatcccg atcatcgccc tcgcgccgtt gatcgtgttc gcgctcggcc agggcgtgct
     2641 ctcggtcgtc gtcatcgcga cctacctcac gttcttcccg gtcacgatcg ccatgctccg
     2701 cggcctgcgc tcattcgacc cgcgtgccct ggagctgatg cggtcctatg gggcgtcgaa
     2761 gaccgggatc tacaccaagc tgcggctgcc ggcgtcgatg ccgtacttct tcaccgcgct
     2821 caagatcgcc gccaccgcga gcatcgtggg cgcgatcatc gccgaggaca ccggcgccat
     2881 caaggagggc ctcggccgcg tgatcacgac cttcaaccag tactacgcaa cgggcccgga
     2941 gaagctatgg gcggcgatct tcatcgccgg cgtgctcggg atcggcttct tcctcgtcat
     3001 ccgcgcggcc gagatcatcg tgctccgcgg acgcccgggg atcgacgcgt gaccgacggc
     3061 accgtgcccg cccccgccgc gcccatcgtc cggatcaagg gcgtcgacaa ggtgttcacg
     3121 acgcgcgagg gcgcgagcac cgtcgccctc caggggatcg acctcgagat ccgcccgcgc
     3181 gagttcgtgt ccctcatcgg tccgtcgggc tgcggcaagt cgacgttgct gcgggtcatc
     3241 ggcgacctcg tccagcccag tcgcggagag gtcgtcgtca acggcaagcc ggcgccacgg
     3301 gcccgcctcg accgcgacta cgggatggtc ttccaggccc cggtgctgtt cgactggcgc
     3361 agcgtcgagg acaacgtcaa gttgccgctc gagatcctgg gcaagcccaa gtcctggcgc
     3421 acgcagcggg ccaaggagat gctcgacctg gtcgagctcg gcgagttcac ccgccatatg
     3481 ccctaccagc tctcgggcgg catgcagcag cgggtcgcga tcgctcgcgc gctgtcattc
     3541 gagccccgga tcctgctcat ggacgagccg ttcggggcgc tcgacgagat gacccgcgag
     3601 cggatgaacg acgaggtgct ccggatctgg gagcagaccg gcacgacgat cgtgttcgtg
     3661 acccactcga tcccggaggc cgtgttcctc tcgtcgcggg tcgtcgtgat gagcgcccgg
     3721 ccgggccgga tcacgaaggt cgtcgacgtc gacctgccgc ggccccgaac ggacgaggtt
     3781 cgcgagaccg aggcgtactt ctcgctgatc accgaggtcc gcgaggcact ccgggccggc
     3841 ggctcggacg gcgccgacga ggacgggtca gcgtccagcc gacggacgca ggccgagggc
     3901 gccgtcgggt gagcgcagcg acggggatgg tgggtcccag cagcagcgac accgggccgg
     3961 gccgggccgc ccgggccgcc cgcgtggccc gcgagtacct gccggccgtg ctcgtcgcgg
     4021 tcggcgcgct gatcgtctgg gagggcctga tccgcgcgct ccagatgcgc gcgttcatcc
     4081 tgcccgcgcc atcggcgatc ctcgccgccc tggccgagaa ctggagcggg gagcggttcc
     4141 cgctgttccg ttcggcgcag tcgacgatct tcgaggcgat cggtgggttc gtcatcggcg
     4201 tgacggcggg cacggccgtc gcgttcatga cggcgcgctg ggccgggatg cgctcggtga
     4261 tgctgccggc ggcggtggcg gcaggggcga tcccgatcat cgccttcgcg ccgctgttca
     4321 acacctggtt cgggctgacc aacccgctgt ccaagatgat gatggcggcg gtcctgtcgt
     4381 tctttccggt gatggccaac gtgacccggg gcctcgccgc ggtggacccg agctcgctcg
     4441 agctgatgcg ctcctacgcc gcatcggagg ggacggtcct gcgcaaggtg cgcatcccca
     4501 acgccctccc gtacttcttc acggcgctca agctggcctc gacgctcagc ctcatcgggg
     4561 cgatcgtcgc cgagtacttc ggcggctcga cgtcggtcct cggccggatg atcgtccaga
     4621 gtgcgagcgc cctgcggttt gacatcacct gggccgcggt gtcgctcgcg gcggtgaccg
     4681 ggatcgtgtt ctacctggtc atcgcactcg tcgagcggct ggtcatcccc tggcacagct
     4741 cggcgcgcaa cgaggcctga cggcccggtc gcgacccgcc ggtgaggccc gtttcgcccg
     4801 gctcgccaat ccggtatcgt tgccgccgat ccgcggctca tggccgcggc ggaggagaga
     4861 agtgggcgtc ccgcgcgtcg gggcgccgca acagaggaga agcatgagga agcagacacg
     4921 cgtcctcggg ctggtgtccg cagcggcggt gctgttcgca gcgtgctcga gctcgaccgc
     4981 gactccc
//
LOCUS       DBNHXO010000544         3031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1575794, whole genome shotgun sequence.
ACCESSION   DBNHXO010000544 DBNHXO010000000
VERSION     DBNHXO010000544.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1575794"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1440
                     /locus_tag="AC162A_10990"
     CDS             <1..1440
                     /locus_tag="AC162A_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5011412.1"
                     /translation="TMKVASVVGRVFEAPMLPGAYEELGDLDAVLGHLDALRTLDLVA
                     LDREAEQAWMFKHVVTQEVAYESLPFALRAVLHGRVGDHIERTEADDLERAVPLLEHH
                     YWRSDREDKKVLYLRLAAEQAQASYANQAAIAYYDRLVPLLEGGEQVQAMLGLGKVLD
                     LVGDWSRARMVGEDALREATTLGDGGAQGWALAALADIDRRQGQFDDAREALSNAADL
                     FRAAGDEAGEGQVLHLAGTVAAQTGDLDLARARYEESLLIRERIGDRAKVGSLYSNLA
                     IVAEYAGDQDETVRMAELALAVREEAGDTWGVGVSHTNLGWIAIRAGQLAEARTHLET
                     AIGVMQQVGDTWFTTSARALLANVDRDLGDLAAAGRGYAESLRVFVTLNDQVAQVEVL
                     EDVATLATRLGDHEAAVALASAITAVRDELGSPMAEDLAAEHRGRLEPSVEALGDEGA
                     AAAEARGRRLGIDGAVALALGVCTPAAEA"
     gene            1679..>3031
                     /locus_tag="AC162A_10995"
     CDS             1679..>3031
                     /locus_tag="AC162A_10995"
                     /inference="COORDINATES: protein motif:HMM:NF016733.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF642 domain-containing protein"
                     /protein_id="HIR5011413.1"
                     /translation="MVRPFRPLFNPRNVLWRLGFVAPVAMLALAAIASPLGVLASGRT
                     TWVGDARPGFEANLVDATVVKDGAGGLSEFHYRYDACTGPLTPAGCGGPGLTERQLYF
                     ATTADAKESVRLDWKFTGFHAFAGVEVGLEANVISVLGEDTNIPLVADGPVSCCTPPS
                     GGFTYSDTVHLDVEPGDTFGFTITGSNGDATSILEGTLTVATSVVVNGSFEQGEMTSP
                     TTFDRLDADSTDLTGWTIADGNVDWTGGRWQASDGVRSLELVGSGPGEIYQDLPTIDG
                     QTYDVRYAYSANPGRNGEPLAPDCATVANPSPTYGFALGSTVFTGPGNTFTAANTPAD
                     MKWQHANLEFTATESVTRVRFFSTDASGCGIVIDDISVVPVIPTDPGPTPSPSPGPSE
                     ETDPTQDVFAMTVTTADDHDNGFCTVSDCSLREAINAANAAGLNEGAYGIGFNVDGAS
                     V"
BASE COUNT          480 a         1047 c         1068 g          436 t
ORIGIN      
        1 acgatgaagg tcgcgagcgt cgtcggacgc gtgttcgagg cgccgatgct gccgggggcg
       61 tacgaggagc tcggcgatct cgacgccgtc ctcggacacc tcgacgcgct tcgcacgctc
      121 gacctcgtgg cgctcgaccg ggaggccgag caggcctgga tgttcaagca cgtggtgacc
      181 caggaggtcg cctacgagag cctgccattt gccctccgcg cggtcctcca cggccgtgtc
      241 ggcgaccaca tcgagcgcac ggaggccgac gatctcgagc gcgccgtgcc cctgctcgag
      301 catcactact ggcggagcga ccgcgaggac aagaaggtcc tgtacctgcg cctggcggcc
      361 gaacaggccc aggcgtcgta cgcgaaccag gccgcgatcg cctactacga ccggctcgtg
      421 ccgctgctcg aggggggcga gcaggtccag gcgatgctcg gactgggcaa ggtgctggat
      481 ctcgtgggtg actggtcccg agcgagaatg gtcggcgagg acgcccttcg cgaggcgacg
      541 acgctcgggg atggcggcgc ccagggctgg gcgcttgcgg cactcgcgga catcgatcgc
      601 aggcagggcc agttcgacga cgcgcgcgag gcgctctcga acgccgcgga tctgttccgg
      661 gcggccggcg acgaggccgg cgaaggccag gtgctgcacc tggcggggac cgtcgcggcg
      721 cagaccggag acctcgatct cgcccgggct cgatacgagg agagcctgct catccgggag
      781 cggatcggtg atcgcgcgaa ggtgggctcg ctgtactcca atctcgcgat cgtggccgaa
      841 tacgcgggcg accaggacga aaccgtgcgc atggctgagc ttgcgctcgc ggttcgcgag
      901 gaggcggggg acacctgggg cgtcggcgtc tcgcacacca acctcggctg gatcgcgatc
      961 cgggccggcc agctcgccga ggccaggacg catctcgaga ccgccatcgg ggtcatgcag
     1021 caggtcggcg acacgtggtt cacgacgtcg gcccgggcgc tgctggcgaa cgtggatcgc
     1081 gacctcggcg acctcgccgc cgcgggccgg gggtatgccg aaagcctgcg ggtgttcgtc
     1141 acgctcaacg accaggtggc ccaggtcgaa gtgctcgagg acgtcgctac cctggccact
     1201 cgtctggggg accatgaggc cgcggtcgcg cttgcgtccg caatcacggc cgtccgagac
     1261 gagctcggct cgcccatggc ggaggacctg gcagccgaac atcgggggcg cctcgagccc
     1321 agcgtcgagg cgctgggcga cgaaggcgcg gccgcggcgg aagctcgcgg tcggaggctc
     1381 ggcatcgatg gtgccgtcgc gctcgcgctg ggcgtctgca cacccgccgc cgaggcctga
     1441 gctccgcgcg ccggtggtgc gcggcgacac cgccgggagc gcttcgaaac ccaatcaacg
     1501 cattgcgccg accccccacg gcgactaacg taaccggact acgccgcacc aaaccaaacc
     1561 gcgtgaccaa cggccggtca cggcggttgt catcgcgtct gcgcaaccct cagtgcccga
     1621 ggtcaggaac gtgagtcacc cgcgcccctc gtcgtccgac gtcaccggac gacccgtagt
     1681 ggtcaggccc ttccgtccgc tgttcaaccc ccggaacgtg ctctggcgcc tcggctttgt
     1741 tgcgcccgtc gcgatgctgg cgctcgccgc gatcgcgtcg ccgctgggcg tgctggcctc
     1801 gggccggacg acgtgggtcg gtgacgccag gccgggtttc gaggccaatc tcgtcgacgc
     1861 gacggtcgtc aaggacggcg cgggtgggct ctcggagttc cactaccgct acgacgcgtg
     1921 cacgggtccc ttgacgcccg ctggatgcgg cggtccaggc ctcactgagc gccagctgta
     1981 cttcgcgacg acggccgacg cgaaggagtc ggtccggctc gactggaagt tcaccggatt
     2041 ccacgccttt gcgggcgtcg aggtgggcct cgaggcgaac gtgatcagcg tcctcgggga
     2101 ggacacgaac attccgctgg tcgccgacgg gcccgtgtcg tgctgcacgc caccgtcggg
     2161 aggcttcacc tatagcgaca ccgtgcatct cgatgtcgag cccggcgaca ccttcgggtt
     2221 caccatcacc ggttccaacg gcgacgcgac ctcgatcctc gaagggacgc tgaccgtcgc
     2281 aacgagcgtc gtcgtcaacg gctcgttcga gcagggcgag atgacgagcc ccaccacgtt
     2341 cgaccggctc gatgcagact ccacggactt gacaggctgg acgatcgccg acggcaacgt
     2401 ggactggacc ggcggtcgat ggcaggccag tgacggcgtc cgatcgctgg agctggtggg
     2461 tagcgggccg ggcgagatct accaggacct gccgacgatc gacggccaga cgtacgacgt
     2521 ccggtacgcg tactccgcca acccgggccg caacggcgag ccgctggcgc ccgactgcgc
     2581 cactgtcgcc aacccgtcgc ccacgtacgg tttcgcgctg ggcagcaccg tcttcaccgg
     2641 acccgggaac acgttcacgg cggccaacac ccccgccgac atgaaatggc agcatgccaa
     2701 cctggagttc accgcaacgg aatcggtgac ccgggtgagg ttcttctcga cggacgccag
     2761 cggctgcggg atcgtcatcg acgacatctc cgtcgtgccc gtgatcccga ccgatcccgg
     2821 tccgacgccg tcgccgtcgc cgggcccgag cgaggagacg gacccgacgc aggacgtgtt
     2881 cgcgatgacg gtcacgaccg ccgacgacca cgacaatggc ttttgcacgg tctcggactg
     2941 ctccctccgc gaggccatca acgccgcgaa tgcggcaggc ctgaacgagg gcgcctacgg
     3001 catcggcttc aacgtcgacg gcgcgtcggt g
//
LOCUS       DBNHXO010000545         2283 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2368832, whole genome shotgun sequence.
ACCESSION   DBNHXO010000545 DBNHXO010000000
VERSION     DBNHXO010000545.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2368832"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="AC162A_11000"
     CDS             complement(<1..463)
                     /locus_tag="AC162A_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5011414.1"
                     /translation="MSPIDPLEVPSHPHDVLVIGAGGAGLRAAIEAQAAGADVGLVCK
                     SLLGKAHTVMAEGGVAAALAHVAAGDTWQVHFRDTMVGGKLLNNPRMAQLHATEAPDR
                     VRELELWGAVFDRTDDGRILQRPFGGHTHPRLAHVGDRTGLEMIRTLQDRAV"
     gene            complement(477..1283)
                     /locus_tag="AC162A_11005"
     CDS             complement(477..1283)
                     /locus_tag="AC162A_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HIR5011415.1"
                     /translation="MSGIPASVRLPRKLPGTAPAGLGTTRRRDRWWIPPLGQGTLFTV
                     LATYLFFSGIVWTPLFGTAYEVDGYLSPLFSPLIAPAWLPAFISPGLLILWIPLGFRA
                     TCYYYRKAYYRFYFADPPGCAVGEPTVHRRSSMESKFPFILQNFHRYFLYLAFVPLFF
                     LWVDAVASLFPASGPRLGLGSVIFFVNVALLSAFSLSCNSLRHLVGGRVDCFSCTRNR
                     RVQYTLWQRITRLNLHHMRWAWVSFISVVLTDVYVRALALGVITDPAIRF"
     gene            complement(1280..>2283)
                     /locus_tag="AC162A_11010"
     CDS             complement(1280..>2283)
                     /locus_tag="AC162A_11010"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HIR5011416.1"
                     /translation="DGIGPELAEATRRVLDASGVAFEWELQDAGEAVIAQYGTPLPEH
                     VLESVRRNKVALKGPITTPVGSGFRSVNVTLRQTLGLYANLRPARSIQGLETRYADVD
                     LVIVRENTEDLYAGIEHMVGPDAAESIKIITRAASERIARFAFDYAVANGRHKVTAVH
                     KANIMKLSDGLFLESCRTVAADYDGRIEFEDRIVDNMCMQLVQKPDLYDVLVLPNLYG
                     DIVSDLCAGLVGGLGVAPGANIGTEASVFEPVHGSAPKYAGQDKANPTAMILSGVLML
                     RQLGEQPAAERVETAVRDVIAEGKHTTYDLGGNAGTRAYADAIIDRLGAPASTSAPR"
BASE COUNT          350 a          789 c          827 g          317 t
ORIGIN      
        1 ccacggcccg gtcctggagc gtgcggatca tctcgaggcc ggtccggtca ccgacgtggg
       61 ccagccgcgg atgcgtgtgg ccgccgaacg ggcgctggag gatccggccg tcgtcggtcc
      121 ggtcgaacac ggcgccccac agctcgagct cgcggacccg gtccggcgcc tcggtcgcgt
      181 ggagctgcgc catgcgcggg ttgttcagca gcttgccgcc gaccatcgtg tcgcggaagt
      241 ggacctgcca cgtgtccccg gcggcaacgt gggccagcgc cgccgcgacg ccgccctcgg
      301 ccatcacggt gtgggccttg cccagcagcg acttgcacac gaggccgacg tctgccccgg
      361 ccgcctgcgc ctcgatggcc gcgcgcagcc ccgcgccacc ggcgccgatg accaggacgt
      421 cgtgcgggtg ggacgggacc tcgagcgggt cgatcggtga cacggttcgt cgccggctag
      481 aagcggatcg ccgggtcggt gatgacgccc agcgcgaggg ccctgacgta gacgtccgtc
      541 agcacgaccg agatgaagct cacccacgcc caccgcatgt gatggaggtt gagcctcgtg
      601 atccgctgcc acagcgtgta ctggacccgc cgattccggg tgcacgagaa gcagtccacg
      661 cgcccgccga ccaggtggcg cagcgagttg cacgacagcg agaaggccga cagcagggcg
      721 acgttgacga agaagatcac gctcccgagg cccaggcgcg gcccgctggc cgggaacaac
      781 gacgcgaccg cgtcgaccca caggaagaac agtggcacga acgcgagata caggaagtag
      841 cggtggaagt tctggaggat gaacgggaac ttggactcca tgctggaccg ccggtggacc
      901 gtcggctccc ccaccgcgca gccgggcggg tccgcgaagt agaagcggta gtacgccttc
      961 cggtagtagt agcaggtggc ccggaagccg agcgggatcc acaggatcag caggcccggg
     1021 ctgatgaagg cgggcagcca cgcgggcgcg atcagcggcg agaacagcgg cgacaggtag
     1081 ccgtccacct cgtacgcggt cccgaacagc ggcgtccaga cgatcccgga gaagaacagg
     1141 tacgtcgcga ggaccgtgaa cagcgtcccc tgaccgagcg gcgggatcca ccagcggtcg
     1201 cgccggcgcg tcgtccccag cccggccggc gccgtgcccg gcagcttgcg cggcaggcgg
     1261 acgctggccg ggatcccgct catcgcggcg cggaggtcga cgccggggcg ccgaggcggt
     1321 cgatgatcgc gtcggcgtag gcgcgggtgc ccgcgttgcc gccgaggtcg taggtcgtgt
     1381 gcttcccctc cgcgatcacg tcgcgcacgg ccgtctcgac ccgctccgcg gccggctgct
     1441 cgcccagctg gcggagcatc aggacgcccg acaggatcat cgccgtcggg ttggccttgt
     1501 cctggcccgc gtacttgggt gccgagccgt ggacgggctc gaacaccgac gcctcggtcc
     1561 cgatgttggc gcccggcgcg accccgaggc caccgaccag gcccgcgcac aggtcgctga
     1621 cgatgtcgcc gtacaggttg ggcagcacga gcacgtcgta caggtccggc ttctggacca
     1681 gctgcatgca catgttgtcg acgatgcggt cctcgaactc gatccggccg tcgtagtccg
     1741 cggccaccgt cctgcagctt tcgaggaaca gcccgtcgga cagcttcatg atgttcgcct
     1801 tgtgaacggc cgtgaccttg tgccggccgt tggcgaccgc gtagtcgaac gcgaacctgg
     1861 cgatccgctc cgacgcggcc cgcgtgatga tcttgatgct ctcggcggcg tcggggccga
     1921 ccatgtgctc gatgccggcg tacaggtcct cggtgttctc gcggacgatc acgaggtcga
     1981 cgtccgcgta gcgggtctcg aggccctgga tcgaccgggc gggccggagg ttggcgtaca
     2041 ggcccagcgt ctggcgcagc gtgacgttga cggagcggaa gccagagccg acgggcgtcg
     2101 tgatcgggcc cttgagcgcg accttgttgc ggcgcaccga ctccagcaca tgctcgggca
     2161 gcggcgtgcc gtactgggcg atcacggcct cgcctgcgtc ctggagctcc cactcgaagg
     2221 cgacacccga ggcgtcgagg acgcgccggg tggcctccgc cagctccggc ccgatgccgt
     2281 cgc
//
LOCUS       DBNHXO010000546         9150 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3957655, whole genome shotgun sequence.
ACCESSION   DBNHXO010000546 DBNHXO010000000
VERSION     DBNHXO010000546.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 9150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9150
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3957655"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..894)
                     /locus_tag="AC162A_11015"
     CDS             complement(16..894)
                     /locus_tag="AC162A_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011417.1"
                     /translation="MSRRVPAILLVLVALAGCAPADAVEPPRLAVVTDVPATPVATPL
                     DAPTARLTAEPAPATEPPATSEAPASPQPGATPEPSATPEPVVGHDTGGIKPSAAMSV
                     NLAQTADFVPQYTFEWCVGASLQMTRSIITGDRNETRRSQGRLWRMARERTLNSPYGG
                     VNPIGWTAALNDMGLGPYRFVSLPTFDAALTAAARAIAVTRRPVGLVMWAGRHAWVMT
                     GFESDGNPRKDDNARVTRIRVMDPLYPHGSRWGRSPAPNTLVSRKTLARQFVLRYRPD
                     YDLGIEPGWLLILPVR"
     gene            983..1696
                     /locus_tag="AC162A_11020"
     CDS             983..1696
                     /locus_tag="AC162A_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2161 family putative PD-(D/E)XK-type
                     phosphodiesterase"
                     /protein_id="HIR5011418.1"
                     /translation="MPASRTRTTTPPETALYGPVKALLEGQGYVVKGEITGCDVVAVR
                     GDEPPVIVELKRTFGLDVVLQGIDRLALTDAVYVAVGAWPRRPADARRLCRRLGLGLI
                     VVVGGRADVVLDPVPYQPRRNRRRTARLLDEHRRRVGDPTSGGSVRRPIMTAYRQEAL
                     RCLERLEAGPATLRAIRAGGDVPHAGPIMQSNVYGWFERVERGVYALSPRGRQALADD
                     RPTGGTPIGGPVSPRSTPR"
     gene            complement(1656..2450)
                     /locus_tag="AC162A_11025"
     CDS             complement(1656..2450)
                     /locus_tag="AC162A_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5011419.1"
                     /translation="MALSVLAIVWLYPVVWTLTNALKSSADIYRAPWDVPWPPQLGNI
                     PDAWNRGQLGLAMANSAIVTALTVAVVLVLAVSGAYALTRLRPPGRAVLFLVVLAPLI
                     VPMEVLIVPLFSMYRALGLINSLPGLAMTNVVASVSFATVILAGYFRTIPQEVIDAAR
                     VDGAGRVSVLLRIVVPLARPGIVAVAVIVAVFAWNDFSGALVLLQRPDTFTVQLALTR
                     FSTFYATDQGLTFAGMAIAIVPPLVLFLLLRRSFIGGLSAGTQGRR"
     gene            complement(2480..3373)
                     /locus_tag="AC162A_11030"
     CDS             complement(2480..3373)
                     /locus_tag="AC162A_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5011420.1"
                     /translation="MSRRGRREGAIDLLFLVPTLAILAVFLVYPLIYGLVLSVHDTHG
                     FDLTAFVGLEHYARAILGDAVFHRSVLNTLLFTGVAVVLQTGLGLLLAILVGGVRRGR
                     ALFVLVFFAPFVLAPVAVGAVWKFLFAPYFGIVPTVGAALGLDTQTFAPLADVDVALW
                     AIMAAFLWRFAGFSMVVYLAAIQALPREYDEYAILEGAGRFHRLRRVTWPLLWPQTFT
                     LVLLTTLGTLRIFDMVWIMTAGGPSHATATVATDVYETAFRFLEVGYAQAMAMVLLVL
                     ILLLAVVEYRILNRRAEAVST"
     gene            3540..4949
                     /locus_tag="AC162A_11035"
     CDS             3540..4949
                     /locus_tag="AC162A_11035"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5011421.1"
                     /translation="MIEPTHPTPDGPWDRPRHRREILKLGAVLAAGAAAAPLVAACAM
                     SATGSPRSPGGTGAGLSGPITVVTGAGGDPTVRPALQRVYDGFKARNPAIEWDIRAIP
                     GGGPEWDRLARALVASGEPVGLVIIDGQQVRGWLRDGLLADLGADPGVAAVLARVPDQ
                     YHHLSGPGETTTRAFPLALTGGVHTTGIYYNKALLDAASLGPPTSIADLKAMVKPLAA
                     LGAAPLVHCSGDVFFNQILMTWVLPMIAERTRDPLEFAESTVRGDIAYDSPEWLEAFE
                     TIADLRSSGVLLEGSGATDYAAMQQLLLQGKAAMTFNGSWLLPQLLAGSPSVPFDLHV
                     VAPPLVDGAARPRPILSWAGVALPASTAASRDSVYAFLDYASQPAVDRTIVEGLQVYS
                     PIAASNDAITNAVAQEFLPLFEDAITPLDWLWEPEITAEIDSQVQGLVKGQGDPRSAA
                     QAVEAVARELHASGRSYYP"
     gene            4975..7713
                     /locus_tag="AC162A_11040"
     CDS             4975..7713
                     /locus_tag="AC162A_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5011422.1"
                     /translation="MPSGLLQTKLYAPRPRGGLVPRPRLTARLDRGIATRLTLVSAPA
                     GFGKTTALAEWLASAPDHRLRTAWLSLDAGDNHPASFWAHVIAALQGVAPGIGAGVLG
                     ALDSSEPSTEIVVAPLLNELSVVTDDIVLVLDDFHVVESPQVLGGIEFLLDHLPANVH
                     LVIATRADPPLPLARLRVRGDLVEVRAADLRFSHGEATAYLTDVMGLALTASDAAALE
                     ARTEGWIAAIQLAGLSMQGRDDVSGFIAGFAGDDRYVVDYLMEEVLRRQPEGVRGFLL
                     ETSILARMTGPLVDAVTGQRGGNATLEALDRGNLFLVPLDDRRQWYRYHHLFGDVLKA
                     RLLDERTADVSDLHRRASDWYELNGERPEAIRHAMAGGDLARAADLVELAMPGMRQRR
                     EEDELRRWLDALPTDLIMERPVLSTGYVGVLLATGELDGVEEFLQSVERWLAATPEAG
                     ERPSGMIIVDEAMVPRVPAGVAMYRAAQARMLGDVPGTMAHARRALDLVAEDDHLGRG
                     GARALLGLAYWTSGDLDSAYRWFADGVENLEQGGHLPDVVGGAITLTDIRLTQGRLTE
                     ALTLFERGLAMATRPGEPVLRGAADMHVGISRILRERNDLGGARQHLRQAEELGEANG
                     LPQNRHRWRISMAQVRQAEGDLDGALELLAEAERLYVGDFSPNARPIAAMKARVWIGQ
                     GRLGEAMGWAREHGISGSDDLTYLREFEHITLARLLLAQGTRDRTGTALDEANGLLVR
                     LLAAAEAGGRTGTAIEILVVLALVREARGDGADAMAALERALALAEPEGYVRVFVDEG
                     PPLGALLRLLAKDRSASSYVRQLLAATVTTARETSSDQPLIEPLSERELDVLRLLESD
                     LDGPDIARELSVSLPTVRTHTRNIYAKLGVTSRRAAVRRAAELGLLARAPEGRPAS"
     gene            7865..8110
                     /locus_tag="AC162A_11045"
     CDS             7865..8110
                     /locus_tag="AC162A_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011423.1"
                     /translation="MNEHRTSRGDRDGAGHYQIRLTGHLDARWATWFDGLSVTHEGDG
                     TTVISGPVADQAALYGLLQRVRDLGLPLVSVTHVDTD"
     gene            8141..8947
                     /locus_tag="AC162A_11050"
     CDS             8141..8947
                     /locus_tag="AC162A_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HIR5011424.1"
                     /translation="MASTTRSAATNRISMSSMRKTALVAGVLYLLTFLSSIPAVLLQS
                     PVLSDPTFIISGISTEQVRLGALLDIVNSLTAIGTAVALYAVVKRQNQGFAIGFVSSR
                     LFEATILMIGVVSILSIVTLQQPGATGAEAALLVTVQDTLVTVRNWSFVLGTGVPALN
                     AVMLGWLMYKSRLVPRAIPVIGLIGAPLFTSWIIGYVLGVTESGTAWHAIGVAPIFIW
                     ELSLGLWMTFKGFRKDAPLMVEAAAEATELDASQGVAHRSFGAATEGGAA"
BASE COUNT         1372 a         3264 c         3227 g         1287 t
ORIGIN      
        1 gggcgaggac gatcctcagc gaacgggcag gatcagcagc cagcccggtt cgatgcccag
       61 gtcgtagtcc ggccggtagc gcagcacgaa ctgccgcgcc agcgtcttgc gcgatacgag
      121 cgtgttgggt gccgggctcc gcccccagcg cgacccgtgc gggtagagcg gatccatcac
      181 gcggatccgg gtcaccctcg cgttgtcgtc ctttcgcggg ttcccgtccg actcgaagcc
      241 ggtcatcacc cacgcgtggc gacccgccca catcacgagg ccgacgggcc ggcgggtcac
      301 ggcgatggca cgggccgccg ccgtgagggc ggcgtcgaac gtcggcaggc tgacgaagcg
      361 atacgggccg aggcccatgt cgttgagcgc tgcggtccag ccgatggggt tcacgccgcc
      421 gtatgggctg ttcagcgtgc gctcccgggc catccgccac aggcggccct gtgagcgcct
      481 cgtctcgttg cggtcaccgg tgatgatcga acgcgtcatc tgcaggctgg caccgacaca
      541 ccattcgaac gtgtactggg gcacgaagtc tgccgtctgc gccaggttca cagacattgc
      601 agccgacggc ttgatgcccc cggtgtcgtg cccgaccacg ggctccgggg tagcgctggg
      661 ctccggggtt gcacctggct gcggcgatgc cggagcctcg ctggttgccg ggggctcggt
      721 cgcgggtgcc ggttcggcgg tcagccgggc cgtcggggca tcgagcgggg tcgcgacggg
      781 cgtcgcgggc acgtcggtca cgacagcgag ccggggcggc tcgacggcat ccgccggagc
      841 gcatccggcc agcgccacga gcacgaggag gattgcgggg acgcgccggg acatggtccg
      901 ggcagtatgc cccgaggtct cggatcacgc tcccggtccg atcggctgtc gcggggatgg
      961 ccgcccgtgg caccgtagat ccgtgccagc gtcgcggacc cgaaccacaa ccccgcctga
     1021 gaccgcgctc tacggaccgg tcaaggcgtt gctcgagggg cagggctacg tcgtcaaggg
     1081 cgagatcacc gggtgcgacg tggtcgccgt ccgcggcgac gagccgccgg tgatcgtcga
     1141 gctcaagcgg acgttcggtc tcgacgttgt cctccagggc atcgaccggc tcgccctgac
     1201 cgacgccgtc tacgtcgcgg tcggtgcatg gccgcgccgg cccgccgacg cacggcggct
     1261 gtgtcgccgc ctcggcctgg ggctcatcgt ggtcgtcggc gggcgcgcgg acgtggtcct
     1321 cgatcccgtc ccctaccagc cacgcaggaa ccgacgccga acggcccggt tgctcgacga
     1381 gcatcgccgg cgggttggtg atccgaccag cggtggctcc gtgcggcgcc cgatcatgac
     1441 cgcctaccgg caggaggccc tgcgctgcct cgagcggctc gaggcgggcc cggcgaccct
     1501 gcgggcgatc cgtgcgggag gcgacgtgcc gcacgcgggt ccgatcatgc agtcgaacgt
     1561 gtacggctgg ttcgagcgcg tcgagcgcgg cgtgtatgcg ctttcgccgc gtgggcggca
     1621 ggccctcgcg gacgaccggc cgacgggagg gacgcctatc ggcggccctg tgtccccgcg
     1681 ctcaaccccc cgatgaagct tctgcgcaga agcaggaaca ggacgagggg cgggacgatc
     1741 gcgatcgcca tgcccgcgaa cgtgagcccc tggtcggtag cgtagaacgt cgagaaccgg
     1801 gtcagcgcga gctggacggt gaacgtgtcc ggccgctgga gcagcacgag ggcaccgctg
     1861 aagtcgttcc acgcgaacac cgcgacgatg accgcgacgg cgacgatccc cggtcgtgcg
     1921 agcgggacca cgatccggag gaggacgctg acccggccgg cgccatcgac cctcgccgca
     1981 tcgatcacct cctgcgggat cgtgcggaag tagccggcca ggatcaccgt cgcgaacgag
     2041 acgctggcga caacgttcgt catcgccagc ccggggaggc tgttgatcag gcccaacgcc
     2101 cggtacatcg agaacaacgg gacgatgagc acctccatcg ggacgatcag cggcgccagg
     2161 acgaccagga acagcacggc ccggccgggc ggtcgaagcc gggtgagcgc atacgccccg
     2221 ctgacggcaa gcacgagcac gacggccacg gtcagcgcgg tcacgatcgc ggagttcgcc
     2281 atcgcgagcc cgagctgtcc ccggttccat gcgtcgggaa tgttcccgag ctggggcggc
     2341 cacggcacat cccacggggc gcggtagata tcggcgctcg acttgagcgc gttggtcagt
     2401 gtccagacca ccgggtacag ccagacgatg gcgagcacgc tcagggccac cgtcgaccat
     2461 cgaacgcgcg gcagcctcgt cacgtgctca cagcctcggc ccgccgattg aggatccggt
     2521 actcgacgac ggccagcagg aggatcagga cgagaaggac catcgccatc gcctgcgcat
     2581 agcccacctc gaggaaccgg aaggcggtct cgtagacgtc ggtggcgacg gtcgcggtgg
     2641 cgtgcgacgg cccgcccgcg gtcatgatcc acaccatgtc gaagatgcgc agggtcccga
     2701 gggtggtcag caggacgagg gtgaacgtct gcggccacag cagcggccag gtgacccgcc
     2761 ggagccgatg gaacctgccg gcgccctcga ggatcgcgta ctcgtcgtac tcgcgcggca
     2821 gcgcctggat ggccgcgagg tacacgacca tgctgaagcc ggcgaaccgc cacaggaagg
     2881 cggccatgat cgcccacagc gcgacgtcga cgtccgcgag tggcgcgaac gtctgggtgt
     2941 cgagcccgag ggccgcgccg accgtcggga cgatcccgaa gtacggcgcg aacaggaact
     3001 tccacaccgc gccgaccgcg acgggggcga gcacgaacgg cgcgaagaac acgaggacga
     3061 acagcgcccg cccgcgacgt accccgccga cgaggatggc cagcagcagc ccgagcccgg
     3121 tctggagcac gacggccacg ccggtgaaca ggagcgtgtt cagcacgctc cgatggaaca
     3181 ccgcgtcgcc caggatcgcc cgcgcgtagt gctcgagccc cacgaaggcc gtgaggtcga
     3241 agccgtgggt gtcgtgcacg ctgagcacga gaccgtagat gagcgggtag acgaggaaca
     3301 cggccaggat cgccagggtc ggcacgagga acagcaggtc gatggcgcct tcgcgccgtc
     3361 cgcgcctgct caccaccagc ccgtgacgat cactcgtggc gcgttccgcc gccgtcgcat
     3421 gcgaattgga cccagatcac ccacgtgccg gttctgacac gccgaacgat acgtgacaat
     3481 gtggtccgga aacgaagcat gagcggcgcc gagctcgtga ctggtcaacg aggagccgct
     3541 tgatcgaacc gacccacccg acacccgacg gcccttggga ccgcccgcgc catcgccgcg
     3601 agatcctgaa gctgggtgcc gtcctcgccg cgggcgcggc ggcggccccc ctggtggccg
     3661 cgtgcgcgat gtccgccacc ggctcgccac gatccccagg tggcaccggc gccgggctgt
     3721 ccgggccgat caccgtggtc accggtgccg gcggcgatcc gaccgtgcgg ccggccctgc
     3781 agcgggtcta cgacggtttc aaggcacgga accccgcgat cgagtgggac atccgggcga
     3841 tcccgggcgg cgggcccgag tgggatcggc tcgctcgcgc attggtggcg tcgggcgagc
     3901 cggtcgggct cgtgattatc gatggtcagc aggtccgggg ctggctgcgg gacgggctgc
     3961 tcgccgacct gggcgcggat ccgggtgttg ccgccgtcct ggcccgggtg cctgatcaat
     4021 accaccacct cagcggtccc ggggagacca cgacgcgcgc cttcccgctg gccctcacgg
     4081 gaggcgtcca caccacgggc atctactaca acaaggcgct gctcgacgcc gcctcgctcg
     4141 gaccgccgac atcgatcgcg gacctcaagg cgatggtcaa gcccctggcc gcgctcgggg
     4201 ccgcgccgct ggtccactgc tcgggtgacg tgttcttcaa ccagatcctg atgacctggg
     4261 tcctgccgat gatcgcggag cggacgcgcg accccctgga gttcgccgag agcacggtcc
     4321 ggggcgacat cgcctacgac agccccgagt ggctggaggc cttcgagacg atcgccgacc
     4381 tgcgctcgtc cggcgtcctg ctcgagggat ctggcgcgac ggactacgcg gcgatgcagc
     4441 agctcctgct gcaggggaag gccgcgatga cgttcaacgg gtcgtggctg ctgccgcagc
     4501 tgctggccgg ttcgccgtcc gtgccgttcg acctgcacgt cgtggcgccg cccctcgtcg
     4561 acggcgcggc gcggccccgc ccgatcctct cctgggccgg cgtcgccctg ccggcctcga
     4621 ccgctgcgag ccgcgacagc gtgtatgcgt tcctcgacta cgcgagccag ccggccgtcg
     4681 atcggacgat cgtcgagggc ctgcaggtgt actcgccgat cgccgcctcg aacgacgcga
     4741 tcaccaacgc ggtcgcacag gagttcctgc cgctgttcga ggacgccatc acgccgcttg
     4801 actggctgtg ggagccggag atcaccgccg agatcgacag ccaggtccag ggcctcgtga
     4861 agggacaggg cgatcctcgg tccgcagccc aggccgtgga agccgtggcc agggagctgc
     4921 acgcctccgg ccgaagctac tacccctgag gcccgcgggt acagtgaccg ggcgatgccc
     4981 agtggcctgc tccagacgaa gctctacgcg cctcggccgc gaggcggcct ggtccctcgt
     5041 ccccggctga cggcgcggct ggatcgaggg atcgcgacga ggctcaccct cgtgtccgcg
     5101 ccggcgggct tcggcaagac caccgcgctg gctgagtggc tggcgtcggc cccggaccac
     5161 cggttgcgca cagcgtggct ctcgctcgac gcgggcgaca accatcccgc gtccttctgg
     5221 gcccacgtga tcgcggcgct ccagggcgtt gcgcccggga tcggcgccgg ggtgctcggg
     5281 gcgctcgact cgtccgaacc ctcgactgag atcgtcgtgg cgccgctgct caacgagctc
     5341 agcgtcgtca cggacgacat cgtgctggtg ctggacgact tccacgtggt cgaatcgccg
     5401 caggtcctgg gcggcatcga gttcctgctc gatcacctgc cggcgaacgt gcacctcgtg
     5461 atcgccacgc gggccgaccc gccgctgccg ctggcgcgcc tgcgcgtgcg cggcgacctc
     5521 gtcgaggtcc gggccgccga cctgcggttc tcccacggcg aggccaccgc ctatctcacg
     5581 gatgtcatgg gcctcgccct gaccgcatcg gacgcggcgg ccctggaggc ccggaccgaa
     5641 ggctggatcg cggcgatcca gctggcgggc ctgtccatgc agggacggga cgatgtctcg
     5701 ggcttcatcg cgggcttcgc cggcgatgac cggtatgtcg tcgactacct gatggaggag
     5761 gttctgcggc gtcagcccga gggcgtccga ggcttcctgc tcgagacatc gatcctggcg
     5821 cgcatgaccg gaccgctggt cgacgccgtc accggccagc gaggaggcaa cgccacgctg
     5881 gaggcgctcg accgggggaa cctgttcctc gtcccgctcg acgaccggcg gcagtggtat
     5941 cgctaccacc acctgttcgg ggacgtcctc aaggcccgcc tcctggacga gcggaccgcc
     6001 gacgtgtccg acctccatcg gcgcgcaagc gactggtacg agctgaacgg cgagcgcccc
     6061 gaggccatcc gccacgcaat ggccggtggc gacctggccc gagcggccga ccttgtcgag
     6121 ctcgccatgc cggggatgcg ccagcgtcgc gaggaggacg agctgcgccg gtggctcgat
     6181 gcgctgccga ccgacctcat catggagcga cccgtgctca gcaccgggta cgtcggcgtc
     6241 ctgctggcga ccggcgagct cgacggcgtc gaggagttcc tgcagtcggt cgagcggtgg
     6301 cttgccgcga cccccgaggc gggcgagcgg ccgtccggga tgatcatcgt ggacgaggcg
     6361 atggtccctc gcgtcccggc gggggtcgcc atgtaccggg ccgcccaggc gcggatgctc
     6421 ggcgacgtgc ccggcaccat ggcccacgcg cgccgcgccc tcgacctcgt cgcggaggac
     6481 gaccatctcg gccgcggcgg cgcgcgtgcg ctgctgggac tcgcctattg gacgagcggg
     6541 gatctcgatt cggcctatcg ctggttcgcg gacggtgtcg agaacctcga gcagggcggg
     6601 cacctgcccg acgtggtcgg gggcgcgatc acgctcaccg acatccggtt gacccagggc
     6661 cggctgaccg aggcgctgac cctgttcgag cgcggcctgg ccatggcgac gcggcccggc
     6721 gagcccgtcc tgcgcggcgc ggccgacatg catgtgggca tcagtcggat cctccgagag
     6781 cggaacgatc tcggtggcgc caggcagcac ctcaggcagg ccgaggagct gggcgaggca
     6841 aacggcctgc cgcagaaccg ccatcggtgg cgcatctcca tggcccaggt ccggcaggcc
     6901 gagggcgacc tggacggggc gctcgagctc ctcgccgagg ccgagcgcct ctatgtcggc
     6961 gacttctccc cgaacgcccg cccgatcgcc gcgatgaagg cgcgggtctg gatcggccag
     7021 gggcggctgg gcgaggccat gggctgggcg cgcgagcacg ggatctcagg cagcgacgac
     7081 ctgacctacc tgcgcgagtt cgagcacatc acgctggcca ggctgctcct cgcccaggga
     7141 acacgcgacc gcaccgggac ggcgctcgac gaggcgaacg ggctcctggt gcgcctgctc
     7201 gccgcggcgg aggccggagg acggaccggc accgcgatcg agatcctcgt cgtcctcgcg
     7261 ctggtccgcg aagcacgcgg cgacggggcg gacgcaatgg ccgcgctcga gcgcgccctg
     7321 gcgctcgccg agccggaagg ctacgtccgc gtcttcgtcg acgaggggcc gccgctcggg
     7381 gccttgctca ggctgctggc gaaggaccgg agcgcttcga gctacgttcg ccagctgctg
     7441 gccgccaccg tgacgaccgc gcgggagacc tcgagcgacc aaccgctgat cgagccgctc
     7501 agcgaacgcg agctcgacgt cctgcggctc ctggagagcg atctcgacgg tccggacatc
     7561 gctcgcgagc tcagcgtgtc cctgcccacc gtgcggacgc acaccaggaa catctacgcc
     7621 aagctcggtg tcactagccg gcgcgccgcg gtccgccgcg ccgccgagct cggcctgctc
     7681 gcgcgtgccc cggagggccg gccagcctcg tagtcctccg tcgcggctgc cacgaaagat
     7741 tcatcacgtc actcatcgcg tgtggtgatg cgcgctcatc acatccgctc cgacgctgcg
     7801 gctgggtcga tcaggtcggg ttcaccgccc gtcggcccag tcgggaacac gcaagaggag
     7861 cggaatgaac gaacaccgca cgtctcgagg cgatcgtgat ggagccgggc actaccagat
     7921 ccggctcacc gggcacctcg acgcgcgctg ggccacctgg ttcgacggcc tgtccgtcac
     7981 ccatgagggc gacggaacca ccgtcatcag cggtccggtc gccgaccagg cagccctcta
     8041 cggactgctc cagcgagtcc gtgacctggg cctccccctg gtctcggtca cccacgtcga
     8101 caccgactag cccacccaac cacgaacaag gagctaccac atggcatcca ccacacggtc
     8161 cgccgcgacg aaccggatct cgatgagctc gatgcgcaag acggcgctcg tcgccggcgt
     8221 cctgtacctc ctcacgttcc tcagctcgat cccggccgtc ctcctccaga gcccggtcct
     8281 gagcgatccg accttcatca tcagcggcat cagcacggaa caggtgcgcc tcggcgccct
     8341 gctcgacatc gtcaacagcc tcaccgccat cgggaccgcg gtcgcgctgt acgccgtcgt
     8401 caagcgccag aaccagggct tcgccatcgg cttcgtgagc tcgcggctgt tcgaagcaac
     8461 catcctcatg atcggcgtcg tgagcatcct gtcgatcgtg accctgcagc agccgggcgc
     8521 cacgggcgcc gaggcggcgc tcctggtcac cgtccaggac acgctcgtca ccgtccgcaa
     8581 ctggtccttc gtcctcggaa ccggcgtccc tgccctcaac gccgtgatgc tcggctggct
     8641 gatgtacaag tcgcgcctgg tcccgcgtgc gatcccggtg atcggcctca tcggagcgcc
     8701 gctgttcacc tcctggatca tcggctacgt cctcggcgtc accgagtccg gcaccgcctg
     8761 gcacgcgatc ggggtcgccc cgattttcat ctgggagctg tcgctcggcc tgtggatgac
     8821 cttcaagggc ttccgcaagg acgcgccgct catggtcgag gccgccgcgg aggcgaccga
     8881 gctcgacgcc tcgcagggtg tcgcccaccg gtccttcggg gccgccacgg agggtggcgc
     8941 cgcctgatgg tccgctgacg gaactccttc ttcgggcgcc acccggcgca atcgccgccg
     9001 ggtggcgcct ccgtgtgcgc ggcaacgccg gcacagggtc cgcggcggta gattgcagtg
     9061 cgtgaggact tcggcgcgct cgagacgacc ggggatgggc gccgtcccgt actcgggtgg
     9121 aaccacgttc cgcgtctggg caccccacgc
//
LOCUS       DBNHXO010000547         2045 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2568610, whole genome shotgun sequence.
ACCESSION   DBNHXO010000547 DBNHXO010000000
VERSION     DBNHXO010000547.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2568610"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..751
                     /gene="ypfJ"
                     /locus_tag="AC162A_11055"
     CDS             8..751
                     /gene="ypfJ"
                     /locus_tag="AC162A_11055"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_02809 family neutral zinc metallopeptidase"
                     /protein_id="HIR5011425.1"
                     /translation="MLSSSGGGGAGSGGLGGVLDGLGGGAVGPVGTPDLTNDGDKVEF
                     IGYVLDDVNALWADVFRDAGDAYQPTTLVLFTSATRSGCGPASSDTGPFYCPADQKVY
                     LDLAFFAELRRRFGAPGDFAEAYVIAHEVGHHVQQQTGIEGQVRQAQQQDPGRVNELS
                     VKMELQADCLAGVWAQSVYEQGDLEAGDIDEGLRAASAVGDDRIQAGAGMAVNPETWT
                     HGSAEQRSSWFDKGYSTGDPAACDTFAGG"
     gene            797..871
                     /locus_tag="AC162A_11060"
     tRNA            797..871
                     /locus_tag="AC162A_11060"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:829..831,aa:Lys,seq:ctt)
     gene            1048..1260
                     /locus_tag="AC162A_11065"
     CDS             1048..1260
                     /locus_tag="AC162A_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011426.1"
                     /translation="MDKRDKRQMLDKLIELKDRCGRLDDEDTTVALLLDDVQLFVAER
                     WAAAVAADAEAAEAAAALVDERAPGR"
     gene            1383..>2045
                     /locus_tag="AC162A_11070"
     CDS             1383..>2045
                     /locus_tag="AC162A_11070"
                     /inference="COORDINATES: protein motif:HMM:NF016176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR5011427.1"
                     /translation="MTTPQPTTRPRGAILVIGVLVLAVAIAGAAGLWYLFFRPAGPAP
                     VSLASLPAVTATQPADSTASPATEPTAGAAATADPAASEDPAATAATSDGIAGAWAVD
                     TSVGSGESGTFVGYRVNEQLATVGAATAVGRTTGVTGTMTIDGTTITAVDITADLTGL
                     QSDESNRDRQLQRQGLETATFPTATFSLTQPIELESVPAEGEIIDVTATGDLTLHGVT
                     KSV"
BASE COUNT          326 a          736 c          711 g          272 t
ORIGIN      
        1 cctcaacctg ctgagcagca gcggcggcgg gggcgcgggg agcggggggc tcggcggcgt
       61 cctcgacggg ctcgggggag gagccgtggg accggtcggg acgcccgacc tgaccaacga
      121 cggcgacaag gtcgagttca tcggctacgt cctcgacgac gtcaatgccc tgtgggccga
      181 cgtgttccgc gacgccggcg acgcgtacca gccgaccacg ctcgtcctgt tcacgagcgc
      241 gacgaggtcg ggttgcgggc ccgcgagctc ggacactggc ccgttctact gccccgccga
      301 tcagaaggtc tatctcgacc tcgcgttctt cgccgagctg cgccggcggt tcggcgcgcc
      361 gggcgacttc gccgaggcgt acgtgatcgc ccacgaggtc ggccaccacg tccagcagca
      421 gacgggcatc gagggccagg tgcgacaggc ccagcagcag gacccgggcc gcgtgaacga
      481 gctgtcggtg aagatggagc tccaggccga ctgtctcgcg ggcgtatggg cacaatcggt
      541 gtacgagcag ggcgacctgg aggccggcga catcgacgag ggcctgcggg cggcgtctgc
      601 cgtgggcgac gaccggatcc aggcgggagc ggggatggcg gtcaacccgg agacctggac
      661 ccacggatcg gccgagcagc gatccagctg gttcgacaag ggctactcga ccggcgatcc
      721 cgcggcctgc gacacgttcg ccggcggctg accagtcgtc ccgcgcggca agtgcggtat
      781 ccttcccgcc ggttccgggc cggtagctca acggtagagc aagtgactct taatcactag
      841 gttcagggtt cgaatccctg ccggctcacc accaccccac gcaggcaaac tgcccgtcaa
      901 tgtcatcggg acggccccga cgttgaccga tcattggggt aggcggcccc cgacaaatca
      961 ggcagttgcc cgatggtcgc gcgcgctccc actcctagcg tgtccccaac gaagccggac
     1021 atccggcaca cgggaaggga gacgacggtg gacaagcgtg acaagcgcca gatgctcgac
     1081 aagctgatcg agctcaagga tcgctgcggt cgcctcgacg acgaggacac gaccgtggcc
     1141 ctgctgctcg acgacgtcca gctcttcgtc gcagagcgct gggccgccgc ggttgcggcg
     1201 gatgccgagg cggccgaggc ggccgcggcc ctcgtcgacg agcgcgcgcc cggacgctga
     1261 tcgccggccg ggtcgggatc tcggcggcga gccgggggta gcgctccgaa ggctctcatg
     1321 cgactctcac ggcgggtcgg ttccataggc acagcaacga acctgcccca ggagcactcc
     1381 tcatgaccac gccgcagccg accacccgac cgcgaggcgc gatcctcgtg atcggcgtcc
     1441 tcgtccttgc cgtcgccatc gccggagccg cggggctctg gtacctgttc ttccgccccg
     1501 ccggccccgc acccgtgtcg ctggcctcgc tacccgcggt gaccgcgacg cagccggccg
     1561 attcgacggc atctcccgcc accgagccga ccgccggcgc cgctgcgacc gccgaccccg
     1621 cggcgagcga ggacccggcc gccaccgcgg caaccagcga cggtattgcc ggcgcctggg
     1681 cggtcgacac gtccgtgggc tcgggcgagt cgggcacctt cgtcggctac cgcgtcaacg
     1741 agcagttggc gaccgtcggc gctgccacgg ccgtcgggcg caccactggc gtcaccggca
     1801 ccatgacgat cgacggcacg acgatcaccg cggtcgatat caccgccgac ctgaccggcc
     1861 tccagagcga cgagtcgaac cgcgaccgcc agctccagcg gcagggcctc gagacggcga
     1921 cattcccgac ggcgacgttc tccctgaccc agcccatcga gctcgagtcc gtgccggccg
     1981 agggcgagat catcgacgtg accgcgacag gtgacctgac gctccatggg gtcaccaagt
     2041 cggtg
//
LOCUS       DBNHXO010000548         3342 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4353704, whole genome shotgun sequence.
ACCESSION   DBNHXO010000548 DBNHXO010000000
VERSION     DBNHXO010000548.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4353704"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="AC162A_11075"
     CDS             <1..1306
                     /locus_tag="AC162A_11075"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:NF049783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR5011428.1"
                     /translation="PNVRPLVQGGDPVGRIRQLAAARERFYAAAARVNGVAELSGVIE
                     AVERFVAAGVPASTTLLHATTKIGEVAFGEGIAADAVSAALQRLEARRAVIMSEPGAW
                     AAFGEGVADGLRARGWPVDEVMLPAGEAAKELAVIGEAARELARLRVERRDPLIAIGG
                     GALGDPAGFLAATYLRGVPWIQVPTTLVAQVDSSIGGKTGVDLPEGKNLLGAFHQPAA
                     IVLDIAALGPLPERQRRAALGEIVKMAALGDEALFATLEADGDAIARGDAGVLASGAL
                     YEAVERPAWAKVEVVSADEREQGATGGRISLNLGHTVAHALEAVDGYATLLHGEAVAY
                     GLRAAVRIGVAAGVTPPDRAARIERLLDDVDLGRSPLPSSPDAVLAATASDKKHRGGV
                     LRWVLPTADGVEVRDDVDAAVVASVIGDVVAGRPAAAAEARA"
     gene            1303..1740
                     /locus_tag="AC162A_11080"
     CDS             1303..1740
                     /locus_tag="AC162A_11080"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR5011429.1"
                     /translation="MTRVLVLEGPNLNLVGTREPEIYGHESLDEIHDWIAVRAAELGL
                     EVDFYQSNHEGALIDRLHRRDFDAAIVNAGGLTHTSIALRDALLAVQRPFWEVHLSDP
                     ATREPFRHVNYLRDIAQASIVGQGARGYHLALEAIAERFGGAA"
     gene            1737..2090
                     /locus_tag="AC162A_11085"
     CDS             1737..2090
                     /locus_tag="AC162A_11085"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIR5011430.1"
                     /translation="MSREGDDPVKPTTAERDEAAVELSRIRDEIDTLDLRIVALLNDR
                     AALGRSAGHAKRLAGRRAIHDPEREREVLLRVAMANRGPIAQADLLSIYRRIVAVTRG
                     LEARDRHLDGRDGDG"
     gene            2161..3060
                     /locus_tag="AC162A_11090"
     CDS             2161..3060
                     /locus_tag="AC162A_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012951.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HIR5011431.1"
                     /translation="MAPVTRFAPAPTGDLHLGHLVNALYTRGIAGATGGRVILRIEDH
                     DRQRSRPTFEAAILDDLERLGLVPDEPTLSAFRSGATPYRQSDASAEYEAAVERLRDA
                     GLVYACACSRATFAAYESERGRPWRGIGCPGDCRPKALPEDEGTGLRVAIGAGSERWI
                     DLLAGPLADEPAAAGDLLVRDRLGNWTYALCVVVDDLRHGVDLVIRGRDLLEATPVQI
                     RLARLLGREVPPAFLHHPLVRRVSGQKLSKSEGDTSVRSLLDAGRTPAELFGLAARLA
                     GLRDDASPVEPADLGSLFELESP"
     gene            complement(3073..>3342)
                     /locus_tag="AC162A_11095"
     CDS             complement(3073..>3342)
                     /locus_tag="AC162A_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011432.1"
                     /translation="PAHCGWQATVWFHRGDALYIRDPEGIFARQTVRPYAEPAKLPSS
                     AVDSGLHSIRWRLFSSPNPKVAWMVRPDGGVEVWARARDPMMGCV"
BASE COUNT          412 a         1195 c         1283 g          452 t
ORIGIN      
        1 gcccaacgtg cggccgctcg tccagggcgg cgacccggtc ggccggatcc gccagctcgc
       61 cgcagcccgc gagcgcttct acgccgccgc cgcgcgcgtg aacggggtcg ccgagctgtc
      121 cggggtcatc gaggccgtcg agcgcttcgt ggcggccggc gtgcccgcct cgacgacgct
      181 cctgcatgcc acgacgaaga tcggcgaggt ggcgttcggc gagggcatcg ccgccgacgc
      241 cgtgagcgcc gcgctccagc ggctcgaggc gcggcgcgcc gtcatcatgt ccgagccggg
      301 cgcctgggcg gccttcggcg agggggtggc cgacgggctg cgtgcgcggg gctggccggt
      361 cgacgaggtg atgctgcccg cgggggaggc ggccaaggag ctcgcggtga tcggcgaggc
      421 ggcgcgggag ctcgcgaggc tgcgggtgga gcggcgggac ccgctgatcg ccatcggcgg
      481 cggggcgctg ggggacccgg ccgggttcct ggccgcgacg tatctccggg gcgtgccgtg
      541 gatccaggtc ccgacgacgc tcgtcgccca ggtggactcg tccatcggcg gcaagaccgg
      601 cgtcgacctg cccgagggca agaacctgct cggggcgttc caccagccgg cggcgatcgt
      661 gctcgacatc gcggccctcg gcccgctccc cgagcggcag cgccgggccg cccttggcga
      721 gatcgtgaag atggcggcgc tcggcgacga ggccctgttt gccacgctgg aggccgacgg
      781 tgacgcgatc gcgcgcggcg acgccggggt gctggcgtcg ggcgccctct atgaggccgt
      841 cgagcgcccg gcgtgggcga aggtcgaggt cgtgtctgcg gacgagcgcg agcagggcgc
      901 gacgggcggg cggatctcgc tcaacctcgg acacacggtg gcccacgccc ttgaggcggt
      961 cgacggctac gcgacgctgc tccacggcga ggccgtcgcg tacgggcttc gggcggcggt
     1021 gcggatcggg gtcgctgctg gggtgacgcc ccctgatcgg gcggctcgga ttgagcggct
     1081 gctcgacgac gtcgacctcg gtcgttcccc gctccccagt tcgcccgacg ccgtcctggc
     1141 cgcgaccgcg agcgacaaga agcaccgggg cggcgtgctg cgctgggtgc tgccgaccgc
     1201 ggacggggtc gaggtccggg acgacgtcga cgcggcggtc gtggcgtcgg tgatcgggga
     1261 cgtcgtcgcc ggccggccgg ccgccgcggc ggaggcgcgg gcgtgacgcg ggtgctggtc
     1321 ctcgagggac cgaacctgaa cctggtcggg acgcgtgagc cggagatcta cggccacgag
     1381 agcctggacg agatccacga ttggatcgcc gtccgcgcgg cggagctcgg gctcgaggtc
     1441 gatttctacc agtcgaatca cgagggtgcc ctcatcgacc gcctccaccg gcgcgacttc
     1501 gacgccgcca tcgtcaacgc cggcggcctc acccacacct cgatcgccct gcgcgacgcc
     1561 ctccttgcgg tccagcggcc gttctgggag gtccacctgt cggacccggc gacgcgcgag
     1621 cccttccggc acgtgaacta cctccgcgac atcgcccagg cgtcgatcgt gggccagggc
     1681 gcgcgggggt accacctcgc gctcgaggcc atcgccgagc gcttcggggg cgcggcgtga
     1741 gtcgcgaggg cgatgacccg gtcaagccga ccacggccga acgcgacgag gccgccgtcg
     1801 agctgtcgcg gatccgcgac gagatcgaca cgctcgacct gcggatcgtc gcgctgctca
     1861 acgatcgggc cgcgcttggg cggtccgcgg gccacgcgaa gcggctcgcg ggccggcgcg
     1921 cgatccacga tcccgagcgc gagcgcgagg tgctcctgcg ggtcgcgatg gccaacaggg
     1981 gcccgatcgc gcaggccgac ctgctctcga tctaccggcg catcgtcgcc gtgacacgcg
     2041 gcctcgaggc gcgcgaccgg cacctcgacg ggcgggacgg cgacggctga cgacgccctc
     2101 gctgacctgc tcgtcccgca ggtcacgggg accgccctgc ccgatccgcg cgtcctgcct
     2161 gtggcgcccg tcacgcggtt cgctccggcc ccgaccgggg acctgcacct cggacacctc
     2221 gtcaacgcgc tgtacacgcg ggggatcgcc ggggcgaccg gcgggcgggt gatcctgcgg
     2281 atcgaggacc acgaccgcca gcggtcgcgg ccaacgtttg aggcggcgat cctcgacgat
     2341 ctcgagcggc tggggctggt tcccgatgag ccgacgctct cggcgttccg atctggcgcg
     2401 acgccgtacc ggcagtccga cgcttcagcc gagtacgaag ccgcggtcga gcggctgcgc
     2461 gacgcggggc tggtgtatgc gtgcgcctgc tcgcgggcga cgttcgcagc gtacgagtcg
     2521 gagcgggggc ggccgtggcg gggcatcggc tgtcccggtg actgccggcc gaaggcactc
     2581 cccgaggacg aaggcacggg cctgcgggtg gcgatcgggg ccggctcgga gcgctggatc
     2641 gacctgctgg cggggccgtt ggccgatgag cccgcggcgg ccggcgatct tctggtacgc
     2701 gaccggctgg gcaactggac gtatgcgctg tgcgtcgtgg ttgacgactt gcgccacggc
     2761 gtcgacctcg tcatccgcgg ccgcgacctg ctcgaggcga cgcctgttca gatccggctc
     2821 gcgcgcctgc tcgggcgcga agtgccgccg gcgttcctcc accacccgct tgtgcgacgt
     2881 gtctccggtc agaagctgtc gaagtccgag ggcgacacct cggtgcgctc gctgctggac
     2941 gccgggcgga cgcccgccga gctgttcggc ctcgccgccc ggctggcggg cctgcgcgac
     3001 gacgcgtcgc cggtcgagcc ggccgatctg ggctccctgt tcgaactaga gtcgccgtag
     3061 gctcccggac cctcagacgc agcccatcat tggatccctg gcccgcgccc acacctcgac
     3121 gccgccgtcc ggccggacca tccaggcgac cttcgggttc gggctcgaga acaggcgcca
     3181 acggatggag tgcagcccgc tgtccaccgc cgacgagggc agcttggctg gctcggcata
     3241 cggacgcacc gtctgacgcg cgaagatgcc ctcggggtcc cggatgtaca gcgcgtcgcc
     3301 gcggtggaac cacacggttg cttgccaccc gcagtgcgca gg
//
LOCUS       DBNHXO010000549         1721 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4154832, whole genome shotgun sequence.
ACCESSION   DBNHXO010000549 DBNHXO010000000
VERSION     DBNHXO010000549.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4154832"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1372)
                     /gene="alaS"
                     /locus_tag="AC162A_11100"
     CDS             complement(<1..1372)
                     /gene="alaS"
                     /locus_tag="AC162A_11100"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR5011433.1"
                     /translation="MASPTDQTAIPYLPAAAIRSRFVEFFAERGHTIVPSASLVPAGD
                     QTLLFTNSGMVQFKDALTGVEKRDYVRAVDYQRCLRVAGKHNDFEEVGRTPRHHTLFE
                     MLGNWSFGDYFKRDAIHWAWQFLTRDLQIPADRLAATVYTTDDLAHSVWKDEIGLPPE
                     RLVRWGDFPAGDEKNWWRMADIGPCGPCSELHYDRGAHLSEGPECVPDHSEHCPRWLE
                     VWNLVFMEFELHPDRSLTPLPAPGVDTGLGLERIASVVQGVTSNYDTDLFAPIHARMR
                     ELLGHDPEGFEQERFSYQVIADHSRAVTFLVGDGVLPANEGRGYVLRRILRRAVRHGR
                     LLGRTEPFMAEAAKVVIDTMADAYPHLGERRDEILGVIAREERQFNRTLEAGTGLLEE
                     ALIPLTGAERVVGRQANTLPPDAPVLPGPVAFKLHDTFGFPIDLTVELAAEYGVRTDL
                     AGFEDAL"
     gene            complement(1444..1650)
                     /locus_tag="AC162A_11105"
     CDS             complement(1444..1650)
                     /locus_tag="AC162A_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011434.1"
                     /translation="MAPERAPDEQRPAGPRPLGSLADLSELLGGDTNRSRRFLGGLMV
                     GALVGAAIAGSTALRLRAKGGRRD"
BASE COUNT          237 a          606 c          628 g          250 t
ORIGIN      
        1 cgagcgcgtc ctcaaagccc gcgaggtctg tgcgcacgcc gtactcggcc gcgagctcga
       61 cggtgaggtc gatcgggaag ccgaaggtgt cgtggagctt gaaggcgaca ggtcccggca
      121 gcaccggcgc gtccgggggc agcgtgttcg cctggcgtcc caccacccgc tccgcacccg
      181 tcagcgggat cagcgcctcc tcgagcaggc ccgtcccggc ctcgagcgtc cgattgaact
      241 ggcgctcctc gcgcgcgatg acgccgagga tctcgtcgcg gcgctcgccg aggtgcgggt
      301 acgcgtcggc catggtgtcg atcacgacct tcgccgcctc ggccatgaac ggctcggtcc
      361 gcccgagcag tcgcccgtgg cggacggcgc ggcgcaggat ccggcgcagg acgtacccgc
      421 ggccctcgtt ggcgggcagc acgccgtcgc cgaccaggaa cgtcacggcc cgcgagtggt
      481 cggcgatcac ctggtagctg aagcgctcct gctcgaagcc ctccgggtcg tggcccagca
      541 gctcgcgcat ccgggcgtgg atcggcgcga acagatccgt gtcgtagttg ctggtcacgc
      601 cctggacgac gctcgcgatc cgctcgaggc cgaggcccgt gtcgacgccc ggcgccggca
      661 gcggcgtcag cgaccggtcc gggtgcagct cgaactccat gaacacgagg ttccagacct
      721 cgagccagcg cgggcagtgc tcgctgtggt ccgggacgca ctcggggccc tcggacaggt
      781 gggcgccgcg gtcgtagtgg agctccgagc agggtccgca gggcccgatg tcggccatgc
      841 gccaccagtt cttctcgtcg cccgcgggga agtcgcccca cctgacgagc cgctcgggcg
      901 gcaggccgat ctcgtccttc cagaccgagt gggcgaggtc gtcggtcgtg tacacggtcg
      961 cggccaggcg atccgcgggg atctggaggt cccgggtcag gaactgccac gcccagtgga
     1021 tcgcgtcgcg cttgaagtag tcgccgaagc tccagttgcc gagcatctcg aacagcgtgt
     1081 gatgccgggg ggtccggccg acctcctcaa agtcgttgtg cttgccggcg acgcggagac
     1141 agcgctggta gtcgaccgcc cgtacgtagt cgcgcttctc gacccccgtc agcgcgtcct
     1201 tgaactggac catccccgag ttcgtgaaca gcagcgtctg gtcgcccgct gggacgaggc
     1261 tggcgctggg cacgatcgtg tgcccgcgct cggcgaagaa ctccacgaac cgcgaccgga
     1321 tcgcggccgc ggggaggtac gggatcgcgg tctggtcggt ggggctcgcc atcgcgcgca
     1381 atggtagcga acggggacct tccaactcgc ccggcgcctc gcccctgcct caacgctcgg
     1441 gtgtcagtcc cggcggccgc ccttggcgcg gagtcggaga gccgtggagc cggcgatggc
     1501 cgcgcccacg agcgcgccga ccatcagccc gccgaggaag cggcgtgatc gattcgtgtc
     1561 cccgcccagc agttcggaca ggtccgcgag cgagccgagc ggccgcgggc cggcgggtcg
     1621 ctgttcgtcg ggcgctcgtt cgggcgccat cagcggacct tctgcgactg cgggcccagg
     1681 gtcgcgagga cggcgacgat gccgatgccg acccaggcca g
//
LOCUS       DBNHXO010000550         2878 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4554293, whole genome shotgun sequence.
ACCESSION   DBNHXO010000550 DBNHXO010000000
VERSION     DBNHXO010000550.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4554293"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2697
                     /locus_tag="AC162A_11110"
     CDS             <1..2697
                     /locus_tag="AC162A_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013124.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5011435.1"
                     /translation="SSSAASLATELLPAGTGLMDLGEHHLRDLGRPEHLYQLVHPDLP
                     AAFPPLVTARQPGADLPSRVAALIGRRDEIAQITERLADGSVRLLTLIGPGGTGKTTL
                     AIRVAADLAPRFRDGVSFVELASAHDTNAVLVAIARSVGLGEIIDRPLQDELVDRLRD
                     TRMLIVLDNLEQVTESGAAVAQLLSDGAGLTVVATSREALHVRAEQVFVVPPLALPPD
                     GRGSVTAASVRQVEAVQLFVDRARVARPDFDLTDDNAAAVAEICRRLDGLPLAIELAA
                     ARLRLFSPEVLRDRLDDSLGLLRGGPRDLPERQQTLRAAMDWSYELLAPGEQRLFEQL
                     AVFADAEVGAIEAVTDEVGAVDGEAIDVLDGLAGLVEKSLLRRIDAPGAEPRVAMLKT
                     IREFATDRLERRHDVDARTRLAHASYYSDVARRLRAELNGARREAALEALHADIGNLR
                     IAWTYWVEARDLEHLDGLAKALLALDDAHGWYLDTAKLAADMLAVVEAVPASPERINQ
                     EIGLRTTLARALMTTKGFTPEVEAAFAGALERFEQGADVGQQFAVLRGLASLYLFRAQ
                     LDKSAAIGQEILLLGEAENDPVMRIDGHLLVGTMLMSYDDLTGGLDHLEQAIALFPDP
                     PTPNRTVRVGNDPRVSTLTTSAFTLWMLGRSDRAAERADAALELAAALDHPFTTAYAR
                     YHAGLLRLWRREPERAHDLAMSLLALADEHEFHVWKATGNVLLGASLVELGRADDGMA
                     AVRAGIGMYGELRSPPIFWPFLLFVQARACASAGRPEEGLRAIDQALDILGQGEGGSI
                     MPEIRILKGDLVQALATDGPTGANDAEPWYRLALDRATELGGLTAQLRAATRLARLRI
                     EAGDPAGAAALLGPVHAAFTEGHDTADVLEATDLLANLSPQD"
     gene            2731..>2878
                     /locus_tag="AC162A_11115"
     CDS             2731..>2878
                     /locus_tag="AC162A_11115"
                     /inference="COORDINATES: protein motif:HMM:NF014643.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR5011436.1"
                     /translation="MRRIGAYEAKTHLPRLLDEVAAGESITITKHGQPVAILVPARPG
                     RAAAA"
BASE COUNT          397 a         1078 c          994 g          409 t
ORIGIN      
        1 tcgtcgtcgg ccgcgagcct cgccaccgag ttgctccccg ccgggaccgg gctcatggac
       61 ctcggcgagc accatctccg cgacctcggc cggcccgagc acctctacca gctcgtccac
      121 ccagacctcc cggccgcctt ccctccgctg gtcaccgcgc gccagccggg cgcggacctg
      181 ccgtcgcgcg tcgccgcgct catcggtcgc cgcgacgaga tcgcccagat caccgagcgt
      241 ctcgccgacg gttccgttcg cctgctcacg ctcatcggcc ccggtggcac cggcaagacg
      301 acgctcgcga tccgcgtggc ggcggacctg gccccgaggt ttcgcgacgg cgtgtcgttc
      361 gtggagctgg ccagcgccca cgacacgaac gcggtcctgg tggcgatcgc gcgctccgtg
      421 ggcctcggcg agatcatcga ccggccgctg caggacgagc tcgtcgaccg cctgcgcgac
      481 acacggatgc tgatcgtgct cgataacctc gagcaggtca ccgagtcggg cgcggcggtg
      541 gcccagctgc tgagcgacgg cgccgggctc accgtggtcg ccaccagccg cgaggccctc
      601 cacgtccggg cggagcaggt gttcgtcgtc ccgccgctgg ccctcccgcc cgacggtcgc
      661 ggcagcgtga cggcggcgtc cgtacgccag gtcgaggccg tccagctgtt cgtcgatcgc
      721 gcccgggtcg cccgtccgga cttcgatctc accgatgaca acgccgcggc cgtcgcggag
      781 atctgccgcc ggctcgatgg cctgccgctg gcgatcgagc tcgcggcggc ccgcctccgg
      841 ctgttctcgc cggaagtgct gcgcgaccgg ctcgacgaca gcctgggcct gctccgcggc
      901 ggaccccggg acctgccgga acgccagcag acgctgcggg cggcgatgga ctggagctac
      961 gagctcctcg cgcccggcga gcaacgcctg ttcgagcagc tggccgtgtt cgcggacgcg
     1021 gaggtgggcg cgatcgaggc ggtcaccgac gaggtcggtg cggtcgacgg tgaggccatc
     1081 gacgtcctcg acgggctggc cggcctcgtc gagaagagcc tcctccgccg gatcgatgcg
     1141 ccgggcgccg agccgcgggt cgcgatgctc aagacgatcc gcgagttcgc caccgaccgc
     1201 ctcgaacgcc ggcacgacgt cgacgcgcga acccgcctgg ctcacgcctc gtactactcg
     1261 gacgtcgcgc gccgcctccg cgctgagctc aacggggccc gacgcgaagc cgccctggaa
     1321 gccctccacg cggacatcgg gaacctgcgg atcgcgtgga cctactgggt cgaggcgcgc
     1381 gacctcgaac acctcgacgg gctcgcgaag gccctcctcg cgctcgacga cgcgcacggc
     1441 tggtacctcg ataccgcgaa gctggcggcc gacatgctgg ccgtcgtcga ggccgtcccg
     1501 gcatcccctg agcggatcaa ccaggagatc gggctgcgga cgaccctcgc ccgggcgttg
     1561 atgacgacga agggcttcac gcccgaagtc gaggccgcgt ttgcgggcgc gctcgagcgg
     1621 ttcgagcagg gcgctgatgt cgggcagcag ttcgcggtcc tgcgcgggct ggccagcttg
     1681 tacttgttcc gtgcgcagct ggacaagtcc gccgcaatcg gccaggagat cctcctcctc
     1741 ggtgaggccg agaacgatcc ggtgatgcgg atcgacggcc acctgctcgt cggcacgatg
     1801 ctcatgagct acgacgacct caccggagga cttgatcacc tcgagcaggc gatcgccctg
     1861 ttcccggatc ccccgacgcc gaaccggacc gtccgcgtcg gcaacgaccc gcgggtctcc
     1921 accctcacca cctccgcgtt caccctgtgg atgctgggca ggtccgaccg cgccgccgaa
     1981 cgcgcggatg cggcgctcga gctcgccgcc gccctggatc atccgttcac gaccgcgtac
     2041 gcccgctatc acgccggcct gctgcgactg tggcgccgcg agccagagcg agcccacgac
     2101 ctcgcgatgt ccctgctggc tctcgccgac gagcacgaat tccatgtctg gaaggcgacg
     2161 ggcaacgtgc tgctcggggc ttcgctcgtg gagcttggcc gggccgacga cggcatggct
     2221 gcggtcaggg ccgggatcgg catgtacggg gagcttcggt cgccaccgat cttctggccg
     2281 ttcctgctgt tcgtgcaggc gcgggcgtgc gcgagcgccg gacggcccga ggagggtctt
     2341 cgcgcgatcg atcaggcgct cgacatcctg ggccagggcg agggggggtc gatcatgccg
     2401 gagatccgga tcctgaaggg tgacctcgtg caggctctgg cgactgacgg gccgaccggc
     2461 gccaatgacg ccgagccgtg gtaccggctg gcgctcgacc gggcgaccga gctggggggg
     2521 ctcacggccc agctccgcgc cgcgacccgg ctcgcccggc tgcgcatcga ggcgggcgac
     2581 ccggccgggg ccgcggccct gctcggcccg gtccacgcgg ccttcaccga gggccacgac
     2641 acggccgacg tcctcgaggc aaccgatctc ctcgcgaatc tgtctcctca ggactagact
     2701 agtcgtagac cagtcgctag catggtcagc atgcgacgga tcggcgccta cgaagcaaag
     2761 acccatctcc cccggctcct cgatgaggtc gccgccggcg agtcgatcac gatcaccaag
     2821 cacggccaac cagtcgcgat cctggtcccc gcgcggcccg gccgtgcggc cgccgcct
//
LOCUS       DBNHXO010000551         2054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_1182573, whole genome shotgun sequence.
ACCESSION   DBNHXO010000551 DBNHXO010000000
VERSION     DBNHXO010000551.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1182573"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /gene="ccsA"
                     /locus_tag="AC162A_11120"
     CDS             <1..266
                     /gene="ccsA"
                     /locus_tag="AC162A_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014367675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HIR5011437.1"
                     /translation="AFPIFATMIILGSWWASIAWSRYWGWDPKETAALVTWLTYAIYL
                     HARNQRRWAGRPAALLLVVGFGMVLITYSGSLWFSGLHAYSGL"
     gene            587..1036
                     /locus_tag="AC162A_11125"
     CDS             587..1036
                     /locus_tag="AC162A_11125"
                     /inference="COORDINATES: protein motif:HMM:NF019428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HIR5011438.1"
                     /translation="MASRGIARRRRGRGERGQSLVEFTLVVPLFLLLIAGMVDFGIGL
                     YSNITVINAAREGARLSVTAPGDPAAVEARVRAMASGLNAADLSVNMTCQRPDPAPAT
                     TWKACTGAQYQPGDAVVVSVDYVYHMIWPLAFGNRIPMTSTVRMRIE"
     gene            1036..>2054
                     /locus_tag="AC162A_11130"
     CDS             1036..>2054
                     /locus_tag="AC162A_11130"
                     /inference="COORDINATES: protein motif:HMM:NF024792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HIR5011439.1"
                     /translation="MTTFGKRTRSGTARDGERGQVIVLFTFFIVVLLGFAAIVVDLGV
                     LRNANQNLWNALDSGALAGASSLPADGTGARALALRYAGANYPDGIDPSRVDLSFRCL
                     IGDRNNNGLPDMTDIPTTCNPGTVAASEWRCARGICAAPCDPAEGDTCNTLVMAGSVS
                     VPFRFGSAVGVTDGNTQVVVSAACKGPCGSEPSALVDVALVVDRTSSMSGVDTDNARN
                     AADSVRKIYNPAAQWLSFGMLGPSQVGGGCSTTPASGIGSAGLPTDLRRWMPIGLTGS
                     GASFGSDYSGATSPLARAIACYTNSSTGTDLTDPIPTAAYELMHNGRSGVTKGIILMS
                     DGQPNA"
BASE COUNT          302 a          744 c          692 g          316 t
ORIGIN      
        1 tcgcgttccc gatcttcgcg acgatgatca tcctcggctc ctggtgggcc tcgatcgcct
       61 ggtcgcgcta ctggggctgg gatcccaagg agaccgccgc gctcgtgacc tggctcacgt
      121 acgcgatcta cctccatgcg cgcaaccagc gtcggtgggc gggtcggccg gccgcgctgc
      181 tgctggtcgt cggcttcggg atggtgctga tcacctattc cggcagcctg tggttctccg
      241 ggttgcacgc ctacagcggg ctgtaaccgg ccacttcggc cgccgttcgt tgcggctacg
      301 ccccaccaac gaaattgggt gaaaggtcct gcgcaaggtc tatcgacccc cgcgtgaccc
      361 tggtgccctg ggcggccggc gccctcgttg tcgcgcggca acgatcccgt tcgggccggg
      421 tcatgggacc ccggcgacgc agcccaaacg gtgcgtgcgc gcatcgggcg atctagtacc
      481 caagtcgcat tggctcggat gactcgcgac ccgaaggatg gcgacaccgg cgacacgggt
      541 gctgccaacc gggagcactg cgaccgccat ccgaagggtt gtccacatgg cgtctcgggg
      601 aattgctcga cgcaggcgcg gacgcggtga acgcggccag tcgctcgtcg agttcacgct
      661 ggtcgtgccg ctgttcctgc tgctgatcgc gggcatggtc gacttcggca tcggcctgta
      721 ctcgaacatc acggtcatca acgccgcccg cgagggcgcc cgcctgagcg tcaccgcgcc
      781 gggcgatccg gccgccgtcg aggcccgggt ccgcgcgatg gcgagcggcc tcaacgccgc
      841 cgacctctcg gtcaacatga cctgccagcg ccccgacccg gcgccggcga ccacctggaa
      901 ggcctgcacc ggcgcgcagt accagcccgg tgacgcggtc gtggtcagcg tcgactacgt
      961 ctaccacatg atctggccgc tggccttcgg caaccggatc ccgatgacct cgaccgtccg
     1021 gatgcggatc gagtgatgac gaccttcggc aagcgaacgc gctcgggaac ggcccgggac
     1081 ggcgagcgtg gccaggtcat cgtcctgttc acgttcttca tcgtcgtgct gctggggttc
     1141 gcggcgatcg tcgtcgacct cggcgtcctg cgcaacgcca accagaacct ttggaacgcc
     1201 ctcgacagcg gcgcgctcgc gggggcctcg agcctcccgg ccgacggcac cggggctcgc
     1261 gccctggcgc tgcgctacgc gggcgccaac tacccggacg gcatcgaccc cagccgcgtc
     1321 gacctcagct tccgctgcct gatcggcgac cggaacaaca acggcctgcc cgacatgacc
     1381 gacatcccga ccacgtgcaa cccgggcacg gtcgctgcct ccgagtggcg atgcgcacgt
     1441 ggcatctgcg cggcgccttg cgaccccgcc gagggcgaca cctgcaacac gctcgtgatg
     1501 gccggctcgg tcagcgtgcc gttccggttc ggcagcgccg tgggcgtcac cgacggcaac
     1561 acgcaggtcg tggtctcggc cgcctgcaag ggcccgtgcg ggtccgagcc atcggcgctg
     1621 gtagatgtcg cgctcgtcgt ggaccgcacg agctcgatga gcggcgtcga caccgacaac
     1681 gcacgcaacg cggccgattc ggtccgcaag atctacaacc cggccgccca gtggctgtcg
     1741 ttcgggatgc tcggacccag ccaggtcggc ggcggctgtt ccacgacgcc ggcgtccggc
     1801 atcggctcag ccggcctgcc gacggacctg cggcggtgga tgccgatcgg gctcaccggc
     1861 tcgggagcct cgttcgggtc cgactactcg ggcgcgacct cgccgctggc cagggcgatc
     1921 gcctgctaca cgaactcgag cacgggcacc gacctcacgg acccgatccc gacggcggcg
     1981 tacgagctga tgcacaacgg ccggagcggc gtgacgaagg gaatcatcct gatgtccgac
     2041 ggccagccga acgc
//
LOCUS       DBNHXO010000552         1851 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_390201, whole genome shotgun sequence.
ACCESSION   DBNHXO010000552 DBNHXO010000000
VERSION     DBNHXO010000552.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_390201"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="AC162A_11135"
     CDS             complement(<1..158)
                     /locus_tag="AC162A_11135"
                     /inference="COORDINATES: protein motif:HMM:NF014045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR5011440.1"
                     /translation="MGKNVAVFVDVANIFYAAKAAGVDIDYVTLLKSATAGRDFVRAY
                     AYTGLDPD"
     gene            complement(210..671)
                     /locus_tag="AC162A_11140"
     CDS             complement(210..671)
                     /locus_tag="AC162A_11140"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5011441.1"
                     /translation="MEYLVRQARITDIDRLATIGRASLTRAGSGSLDAADLMRQLVYL
                     PNASLLVAEVRREIAGGALLVLRPSVVAGGYVGTIDLLVVSPDHDGDRVTDALVEELL
                     RSASNKGCSWVEVSQPSDPTNLARLERIGFSPAGPTLRRPVGAAGAAARRT"
     gene            1016..>1851
                     /locus_tag="AC162A_11145"
     CDS             1016..>1851
                     /locus_tag="AC162A_11145"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR5011442.1"
                     /translation="MKLRAIGALTFAVAVVAAGCTGGSSGEKTLKLGVTLPLSGGAAA
                     DGQPTLKGAQLAVDEINAKGGVGGYKIQLFPLDHAVNGKYNEQQGAQDMQTFIADPAV
                     IGVVGPYNSAVAKVQIPIGNKAGLLQCSPANTNETLTKPEFGALDFRKDFPDRINYIR
                     VAATDDIQGPAMSVYAYNTLGLKNLLIIDDVTTFGTGVANNFQKKFEELGGTTAQRVG
                     APPDTTDFNGIITAAKEKNPDGVYYGGVVTSGAGLLLKQLRQQGLTIPFLGPDGIVNG
                     NG"
BASE COUNT          317 a          625 c          642 g          267 t
ORIGIN      
        1 ttgtccgggt cgagcccggt gtaggcgtac gcgcgcacga agtcgcgtcc ggcggtagcc
       61 gacttcagga gggtgacgta gtcgatgtcc acgcccgccg ccttggcggc gtagaagatg
      121 ttcgccacgt caacgaagac ggcgacgttc ttgcccactg tgactcctat agatggtggg
      181 gcggtccggc catccggccg cccggggcgc taggttcgac gggcggccgc accggccgca
      241 cctacggggc gccggagcgt tggcccggcg gggctgaacc cgatccgctc gaggcgggcg
      301 aggttcgtgg ggtcggaggg ctgcgagacc tcgacccacg agcaaccctt gttgctcgcg
      361 gaccggagga gctcctccac gagtgcatcg gtcacgcggt ccccgtcgtg gtcgggcgag
      421 acgaccagga ggtcgatcgt gcccacgtac ccgccggcga caaccgacgg gcgaaggacg
      481 aggagcgcgc cgccggcgat ctcgcgtcgc acttctgcga cgagcaggct cgcgttcggc
      541 aggtagacga gttggcgcat gaggtctgcg gcgtcaaggg atccggagcc cgctcgcgtc
      601 agcgaagccc ggccgatcgt ggccaggcgg tcgatatccg tgatgcgcgc ctgtcgaacc
      661 aggtactcca cgccgaaggt tggacctcgt gcaagcaagg gcggccggtg cgcttgcgct
      721 cccgtcccga tgaggatcgg aggggggcgg ctacgctgcc gccggaccag gggcccggga
      781 ggcgagcgac tcgacgctcg acagccatcc cgggtggcga tggcgccgcc ttgcatccgc
      841 gacgccagcg cctatgatac ggcatcgctc gaccgctctc gcggcgagcg ttggtttggt
      901 ccctcgctcg cgcacccgtc accgccggaa tcgggcgtac tcccgatcca ccagggacgg
      961 accgcgggtc ccgaggcgag agctctcggc tgcgagggca taggaggagg cgttcatgaa
     1021 gctacgcgcg atcggcgcat tgaccttcgc tgttgccgtg gtggcagcgg gctgcaccgg
     1081 gggcagctca ggcgagaaga ccctcaagct cggcgtcacg ctcccgctgt ccggtggggc
     1141 cgcggccgac ggccagccga cgctcaaggg cgcccagctg gccgttgacg agatcaacgc
     1201 caagggcggc gtcggcggct acaagatcca gctgttcccg ttggaccacg ccgtgaacgg
     1261 caagtacaac gagcagcagg gcgctcagga catgcagacg ttcatcgctg accccgcggt
     1321 catcggtgtc gtcgggccgt acaactcggc cgttgccaag gtccagatcc ccatcggcaa
     1381 caaggctggc ctcctgcagt gcagcccggc gaacacgaac gagacgctga cgaagccgga
     1441 gttcggcgcg ctggacttcc gcaaggactt ccctgaccgc atcaactaca tccgcgtcgc
     1501 ggccacggac gacatccagg gcccggccat gtcggtctac gcgtacaaca cgctgggcct
     1561 caagaacctg ctgatcatcg acgacgtcac gaccttcgga acgggcgtcg ccaacaactt
     1621 ccagaagaag ttcgaggagc tcggcggaac gacggcgcag cgcgtcggcg cgccgcccga
     1681 cacgaccgac ttcaacggga tcatcactgc cgccaaggaa aagaaccccg atggcgtcta
     1741 ctacggcggt gtcgtgacct ccggagcggg cctgctgctc aagcagctgc gccagcaggg
     1801 tctcaccatc ccgttcctcg gccctgacgg catcgtcaac ggcaacggca a
//
LOCUS       DBNHXO010000553         3836 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_189621, whole genome shotgun sequence.
ACCESSION   DBNHXO010000553 DBNHXO010000000
VERSION     DBNHXO010000553.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_189621"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="AC162A_11150"
     CDS             <1..826
                     /locus_tag="AC162A_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011443.1"
                     /translation="AGLGIARRRLGGRMIRRRRLLVGAGIALLMVLAAGSVFGAVAIV
                     NELALGNRPAIASRFGPTDPDAEVPPCTGALAAGSTARLDLRLDASVDDRRTGQVVIG
                     GIRNGRDVRWNGFAATRLLVGQVGAARVGERAWERQPGTAWTETSLGSLENGDLDVPL
                     INLALTPENRSVAEDRGLSYIEGARARHCRITIDGDALRRALPEVSLLIGQTDISRWR
                     VDLDYWVFADGQLGQADGRATGPAGGLESDALLAALRFRLTAVDRGLPVTVFPPTR"
     gene            852..1889
                     /locus_tag="AC162A_11155"
     CDS             852..1889
                     /locus_tag="AC162A_11155"
                     /inference="COORDINATES: protein motif:HMM:NF052219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5011444.1"
                     /translation="MTDAWDGADDPALDGDASDRGDGKRDRLARLLGVLAALQASGEA
                     GIRPDEIARRTGMSKRTVYRDLRALESELRFPVWSEGGRWGVQPGAFLPPLRLTLTEA
                     MAVFLSARLVTRYADKYEPHLASAFQKLEEGLPDALREHVERTLDDLSRRRVDEAFNR
                     HVEDLTRAWAERRVVRFRYAPARYDGTDRKPRWAEVRPYLLEPSLATHALYLIGLDET
                     RDALRTFKVERIIDLSLTPRTFEPPEPGALEATLRRAWDIIADQPETEVVLRFDPSVA
                     ARVLEATWHPSQQVSEAADGSLEWRARVAGTIEIRLWILSWGDEVEVLAPAELRADVA
                     RTLARAIARYP"
     gene            2058..2813
                     /locus_tag="AC162A_11160"
     CDS             2058..2813
                     /locus_tag="AC162A_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR5011445.1"
                     /translation="MLAVTALGGHVSPAAAATSASMADHMLEWMNRDREKRGLVKYRA
                     WGALAVVAEDRAGRMASRNALSHDAAGGNIGAEYDQHGIQWFGYGEIIGVSNADWGND
                     AAKHIYSMWKDSPPHASIMFSNDYNYVGIGFAYRASNETTWASIVFSNSKDHTRPVAE
                     RDGKSVSGRSVTFRWTGHDRALQTHKAGLDDFDVEYRVDGGGWHRIRNGTTARSLTLR
                     DRARGHWYGYRVRASDERGNLSGWTSEGRVWVP"
     gene            2780..>3836
                     /locus_tag="AC162A_11165"
     CDS             2780..>3836
                     /locus_tag="AC162A_11165"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR5011446.1"
                     /translation="MDERGQGLGPVGDEIEPVTDRRGRRLPFAAVNLISDPIHRYVEL
                     TKRLAPDESRAAGLHEEDVAEDDLLDTAWVQRLRRISQLQSARWVFPTAEHSRFTHGL
                     GVMHEAGLWARSLYPSLRSALAGLAPEEPVPSEGLVVETLRIAGLLHDVGHGPFAHFF
                     DERFLASFPAPADPRRPGAKSLTHEDLSQLVIERELGPHIRGLRRAPGAVAERDRFAP
                     GETIEPRWVSFLVSKPPLVDGTMPAWVRILQPLLSGVFTVDNLDYVRRDAYLTGVSMG
                     PVDAERLRRYSFISERGLTLYESGVGALEMFLTARLFMHQHVYLHRTVRAIDLDLAEV
                     FAPSIRAVFGDGSPAERL"
BASE COUNT          522 a         1390 c         1396 g          528 t
ORIGIN      
        1 cgcgggcctg ggcatcgccc ggcggcgcct cggcggccgc atgatccggc gtcggaggct
       61 cctcgtcgga gcggggatcg ccttgctcat ggtgcttgcg gccgggtccg tgttcggcgc
      121 ggtggccatc gtcaacgagc tcgccctggg caatcgcccg gcgatcgcat cccgcttcgg
      181 cccgaccgat cccgacgccg aggtcccgcc ctgcaccggc gcgctcgcgg cgggttcgac
      241 ggcgcgcctc gacctgcggc tcgacgcatc ggtcgacgac cggcggacgg ggcaggtcgt
      301 gatcggtggc atccggaacg ggcgagacgt gcgctggaac ggctttgccg cgacccggct
      361 gctcgtgggc caggtcggcg ccgcacgggt cggcgagcga gcctgggaac ggcagcccgg
      421 gacggcctgg acggagacgt cgctcgggtc gctcgagaac ggcgatctcg acgtgccgct
      481 gatcaacctg gcgctgacgc cggagaatcg gtccgtcgcc gaggatcggg gcctgtccta
      541 catcgagggc gcccgggcgc gccactgccg gatcacgatc gacggcgacg cgctgcgtcg
      601 ggcactgccc gaggtcagcc tgctgatcgg ccagacggac atctcacgct ggcgcgtcga
      661 cctcgactac tgggtcttcg ccgacggcca gctgggccag gccgacggtc gggcgacggg
      721 ccccgcgggc gggctcgagt cggacgcgtt gctggccgcg ctccggttcc ggctcaccgc
      781 cgtcgaccgg gggctgccgg tcaccgtgtt cccgcccacg cgatgagcgg gacgaggagg
      841 aggcgcgtgc catgacggac gcctgggatg gggccgacga ccccgcgctc gacggcgacg
      901 ccagcgaccg tggcgacggc aagcgcgacc ggctcgcgcg cctgctcggg gttctggccg
      961 cactccaggc gagcggggag gccgggatcc gcccggacga gatcgcgcgc cggacgggaa
     1021 tgtcgaagcg gacggtctac cgggacctgc gggcactcga gagcgagctc cgcttccccg
     1081 tctggagcga gggcggccgc tggggcgtcc agcccggcgc cttcctgccg ccgctgcgcc
     1141 tcacgctgac cgaggcgatg gccgtgttcc tgtcggcgcg gctcgtcacg cgctacgccg
     1201 acaagtacga gccgcacctc gcgtccgcgt tccagaagct ggaggagggg ctgccggacg
     1261 cgctccggga gcacgtggag cgcacgctcg acgatctctc ccggcgacgg gtcgacgagg
     1321 ccttcaaccg ccacgtcgag gacctcacgc gggcctgggc ggagcggcgg gtcgtccggt
     1381 tccgctacgc cccggcgcgc tacgacggca ccgatcgcaa gccccgctgg gcggaggtcc
     1441 gtccctacct cctcgagccc tcgctggcga cccacgcgct gtacctgatc gggctcgacg
     1501 agacccgcga cgcccttcgc acgttcaagg tggagcggat catcgatctg tcgctgacgc
     1561 cgcggacatt cgagccgccg gagcccggcg ccctcgaggc cacgctgcgc cgggcctggg
     1621 acatcatcgc ggaccagccc gagaccgagg tggtcctgcg cttcgacccg tccgtcgcgg
     1681 cgcgcgtcct cgaggcgacg tggcatccga gccagcaggt gagcgaggcc gccgacggct
     1741 cgctcgagtg gcgggcccgg gtcgcgggca cgatcgagat ccgcctgtgg atcctgtcgt
     1801 ggggcgacga ggtcgaggtc ctcgcaccgg ccgagctccg cgcggacgtc gcgcgaaccc
     1861 tcgcgcgggc gattgcaagg tacccgtaac ccgcggcggc ccggacttcg ggtaggctgg
     1921 cggcaccccg aaagcccgcg gaccatcgct ccgtcgcccc ccttccggag ggactcgtca
     1981 tcgatccagc tgtcttcggc gttcccgcgc gtcgtcgctc cctgcggcgc catgccggct
     2041 tcgcgatcgc cgccgccgtg ctggcggtga cggcgctcgg cggccacgtg tcgcccgcgg
     2101 cggcagccac ctcggcatcg atggccgacc acatgctcga gtggatgaac cgggaccgcg
     2161 agaagcgagg cctggtcaag taccgggcgt ggggcgccct ggccgtggtg gcggaggacc
     2221 gggccggccg catggcgtct cgcaacgccc tgtcccacga cgcggcgggc gggaacatcg
     2281 gcgccgagta cgaccagcac gggatccagt ggttcggcta cggcgagatc atcggcgtct
     2341 ccaacgccga ctggggcaac gacgccgcga aacacatcta ctcgatgtgg aaggacagcc
     2401 cgccgcacgc gtcgatcatg ttcagcaacg actacaacta cgtcgggatc ggcttcgcct
     2461 accgggccag caacgagacc acgtgggcgt cgatcgtgtt cagcaactcg aaggaccaca
     2521 cgcgcccggt cgccgagcgc gacggcaaga gcgtcagcgg ccggagcgtg acgttccgtt
     2581 ggacgggcca cgacagggcg ctccagaccc acaaggccgg cctcgacgac ttcgacgtcg
     2641 aataccgcgt cgacggcggc ggctggcacc ggatccggaa cgggacgacg gcccgctcgc
     2701 tgaccctgcg ggaccgggca cggggtcact ggtacggcta ccgggttcgg gcgtcggacg
     2761 agcgcggtaa cctctccggg tggacgagcg agggcagggt ctgggtcccg taggggacga
     2821 gatcgagcct gtcaccgacc gccggggtcg ccgcctcccg ttcgccgccg tcaacctcat
     2881 cagcgacccg atccaccgct acgtcgagct gacgaagcgg ctcgcgccgg acgagtcacg
     2941 tgccgccggc ctccacgagg aggacgtcgc cgaggacgac ctgctcgaca ccgcatgggt
     3001 ccagcgactg cggcggatca gccagctcca gagcgcacgc tgggtgtttc cgaccgccga
     3061 gcactcccga ttcacgcacg gcctcggggt catgcatgag gccggcctgt gggcccgctc
     3121 gctgtatccc agcctgcggt cggcgctggc gggcctcgca cccgaggaac ccgtcccatc
     3181 cgaagggctg gtcgtcgaga cgctgcggat cgcggggctg ctccacgacg tggggcacgg
     3241 gccattcgcc cacttcttcg acgagcggtt cctcgcctcg ttccccgccc ccgccgaccc
     3301 gcggcggccg ggcgccaagt cgctgaccca cgaggacctc tcgcagctgg tgatcgaacg
     3361 cgagctcggg ccccacatcc ggggactgcg gcgggcgccg ggggccgtcg cggagcgcga
     3421 ccgcttcgct cccggcgaga cgatcgagcc gcgctgggtc tcgttcctcg tgtccaagcc
     3481 gccgctggtg gacggcacga tgcccgcctg ggtgcggatc ctccaaccgc tgctgtcggg
     3541 cgtgttcacg gtcgacaacc tcgactacgt ccggcgcgac gcctacctca ccggggtgtc
     3601 gatgggaccg gtcgatgccg agcggctgcg gcggtactcg ttcatatccg agcgcgggct
     3661 cacgctgtac gagtcgggcg tgggcgcgct cgagatgttc ctcacggcac gcctgttcat
     3721 gcaccagcac gtctacctgc atcggacggt gcgggccatc gacctcgacc tcgccgaggt
     3781 cttcgcgccg tcgatccggg ccgtgttcgg cgacgggtcg ccggccgagc ggctgg
//
LOCUS       DBNHXO010000554         2420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_190877, whole genome shotgun sequence.
ACCESSION   DBNHXO010000554 DBNHXO010000000
VERSION     DBNHXO010000554.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_190877"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="AC162A_11170"
     CDS             <1..1320
                     /locus_tag="AC162A_11170"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR5011447.1"
                     /translation="DAIGVNCGVGPLACLDALARFGPSADGDPARSIMPNAGLPQRLE
                     GRFIYAAEPPWFGSIVPRLLDAGARIIGGCCGTTPAHIAAMRVALDGSTGAVEPSTAT
                     PSPSIIAGATPRSALAETGPIATIEHERGSRLGHALNAGRFVISVEIDPPRSVRIERT
                     IEAGRLLQDAGADLVNVSDSAMARVRMGAMAVAFGLQHDLDIECLVHVTTRDRNLMAL
                     ESELLGAHALGVRNILALTGDPPRIGDYPTGSGVWDVDSIGLIEILARLNRGEDQAGS
                     PIGQQAVFTIACALDPTATDALTEWDRLERKLAAGAHFVMTQPLYDVAQVEAMFVEAR
                     RRFGPGGFPVPLLLGVLPLVSSRHAEFLHNEVPGITIPESTRARMREAGAAGTEAGID
                     MADALLAAVGDEISGTYLMPSFGRYEQAAELVRRVRTRHPSKAAAAS"
     gene            1349..>2420
                     /locus_tag="AC162A_11175"
     CDS             1349..>2420
                     /locus_tag="AC162A_11175"
                     /inference="COORDINATES: protein motif:HMM:NF016424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR5011448.1"
                     /translation="MRSRHLSRFLSSAAVGAAAIAALAVIAVGPALAAGPPFPPPVDD
                     QAVYDTAGALRDGTIATVEATIDAIEQRTGAEVAVFTQLVPCCETTEDAERNARALMD
                     QWGVGRRGFDDGLVILFDLHEDDPCHGQVQLYAGPGFASRFLTNSERQAIYEDQMLPL
                     LKRCDLDGALLVAMQEVNAAATPEHAAELNFFRLLNAVLGLLIAPLVFILLVGGALFA
                     WFRHGRDPVYLDDPSIHIPAPPVGLTPAAGAVVRDGKSSRRALTAASLDLAARGLIGF
                     QADKAGLLGMSTELSIRTGIASTADPVEAARLARARARPTDEATDFLLQRLRSIGGGA
                     GLIDNKELLKLGSDVGGFDKRLE"
BASE COUNT          318 a          924 c          850 g          328 t
ORIGIN      
        1 gacgcgatcg gggtgaactg cggcgtcggg ccgctcgcct gcctcgacgc gctcgcccgc
       61 ttcgggccgt cggcggacgg cgaccccgcc cgctcgatca tgcccaatgc cggcctgccg
      121 cagcgcctcg agggccgctt catctacgcg gccgagccgc cgtggttcgg ctcgatcgtc
      181 ccgaggctcc tcgacgccgg cgcgcggatc atcggcggct gctgcggcac gacgcctgcc
      241 cacatcgccg cgatgcgcgt agccctcgat ggctccacgg gcgccgtcga gcccagcacg
      301 gccacccctt cgccctcgat catcgccggc gcgacgcccc gcagcgccct cgccgagacg
      361 gggcccatcg cgacgatcga gcacgagcgc ggctctcgcc tggggcatgc gctcaacgcc
      421 gggcggttcg tgatctccgt cgagatcgac cctccgcgca gcgtccgcat cgaacgcacg
      481 atcgaggccg gccgcctcct ccaggacgcc ggcgccgatc tggtcaacgt gtcggactcg
      541 gcgatggcgc gggtgcgcat gggggccatg gccgtcgcct tcgggctcca gcacgacctc
      601 gacatcgagt gcctggtcca cgtcacgacc cgcgaccgga acctgatggc gctcgagtcg
      661 gagctgctcg gcgcccacgc cctcggcgtg cgcaacatcc tcgccctgac cggggatccg
      721 ccgcggatcg gcgactaccc gacggggagc ggcgtgtggg acgtcgactc gatcgggctg
      781 atcgagatcc tcgcccgcct caaccggggc gaggaccagg cgggctcgcc gatcggccag
      841 caggcggtct tcacgatcgc gtgcgcgctg gacccgaccg cgacggacgc gctgaccgag
      901 tgggaccgcc tggagcggaa gctggccgcg ggcgcgcact tcgtcatgac ccagccgctg
      961 tacgacgtcg cgcaggtcga ggcgatgttc gtcgaggcgc ggcggcgctt cgggccgggc
     1021 gggttcccgg taccgctcct gctgggcgtg ctgcccctcg tgtcgtcgcg ccacgccgag
     1081 ttcctgcaca acgaggtccc gggcatcacg atccccgagt cgacccgggc gcgaatgcgg
     1141 gaggcgggcg cagccggaac cgaggccggc atcgacatgg ccgacgcgct gctcgccgcg
     1201 gtcggcgacg agatctcggg cacgtacctc atgcccagct tcggtcgcta cgagcaggcg
     1261 gcggagctgg ttcgacgggt ccggacccgg cacccaagca aggcggccgc ggcctcctga
     1321 cccgatgccc gtcgcgtatc gtggcggcgt gcgcagccga catttgagcc gcttcctctc
     1381 ctcggcagcg gtcggcgccg cggcgatcgc cgccctcgcc gtcatcgccg tcggccccgc
     1441 gctcgcggcc gggccgccgt tcccgccccc ggtcgacgac caggccgtct acgacacggc
     1501 cggcgcgctt cgcgacggga ccatcgccac ggtcgaggcg acgatcgacg ccatcgagca
     1561 gcggaccggc gccgaggtcg cggtcttcac ccagctcgtc ccctgctgcg agacgaccga
     1621 ggacgcggaa cggaacgccc gggcgctcat ggaccagtgg ggcgtcggcc gccgcggctt
     1681 cgacgatggg ctcgtgatcc tgttcgacct ccacgaggac gatccctgtc acggtcaggt
     1741 ccagctgtac gcggggccgg gcttcgcctc gaggttcctc accaacagcg agcgccaggc
     1801 gatctacgag gaccagatgc tgccgctgct caagcggtgc gacctcgacg gcgcgctgct
     1861 ggtcgcgatg caggaggtca acgcggcggc gacccccgag cacgcggcag agctcaactt
     1921 cttccggctg ctcaacgcgg tcctggggct cctcatcgcg cccctcgtct tcatcctgct
     1981 ggtcggcggc gcgctgttcg cgtggttccg ccacggccgc gaccccgtgt acctcgatga
     2041 cccctcgatc cacatcccgg cgccgcccgt cggcctcacg ccggccgccg gcgcggtcgt
     2101 gcgcgacggc aagtcgagcc ggcgcgcgct gacggcggcc agcctcgacc tggccgcgcg
     2161 cggcctgatc gggttccagg cggacaaggc cgggctgctc ggcatgagca cggaactctc
     2221 gatccgcacg gggatcgcgt caaccgccga cccggtcgag gcggcgcgac tcgcgcgcgc
     2281 acgggccagg ccaacggacg aggcgacgga cttcctcctg cagcgcctgc gcagcatcgg
     2341 cggcggggcc gggctcatcg acaacaagga gctgctcaag ctcggctccg acgtcggcgg
     2401 cttcgacaag cggctcgagg
//
LOCUS       DBNHXO010000555         1704 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2769835, whole genome shotgun sequence.
ACCESSION   DBNHXO010000555 DBNHXO010000000
VERSION     DBNHXO010000555.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2769835"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="AC162A_11180"
     CDS             <1..1165
                     /locus_tag="AC162A_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HIR5011449.1"
                     /translation="GAMSAVHDDPDHFRRWTDLPTEAFARRVDYGRYVQEVLAGAIAS
                     SAASLRHVVAQVERLGPEPGGARVTLSNGETLPADVVVLATGVETPALPPYLAALVDD
                     DRVVVDPWVPGALDGIVDGGTVAIIGTSLTAVDLAGSILNRHPRARVIAVSRNGHLPR
                     PHEDPWRPRFPEPPFTVAEFLAFEDPFGEAAARLRASGADWPRAVDSLRPISQALWMA
                     MGDDLRRTFLDDYRHDWEIHRHRMAAEIARDLDAWTTQGRFDVQAAAIEVVEPAGTRL
                     RIRALAAGADKPASWEVDRIIVAIGPNMDPIANPLLGAAIADGILRPGPLGIGIDVDP
                     ATGLVIDANGDTPRPVFAMGALRKGVLWETLAVPEIRGQAHDVASRILGSTAG"
     gene            complement(1411..>1704)
                     /locus_tag="AC162A_11185"
     CDS             complement(1411..>1704)
                     /locus_tag="AC162A_11185"
                     /inference="COORDINATES: protein motif:HMM:NF014173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR5011450.1"
                     /translation="LVKARRGPDGGWSLARAASAISFADVIRAIDGTLVNVAGTRPEK
                     LSYPGSAAGLKTALIAVRAAEREVLESISLADGVSETFPVSVERLLANPNAWH"
BASE COUNT          222 a          612 c          628 g          242 t
ORIGIN      
        1 cggcgcgatg agcgcggtcc acgacgaccc ggaccacttc cggcggtgga cggacctccc
       61 cacggaggcg ttcgcgcggc gcgtcgacta cgggcgatac gtgcaggagg tgctcgcggg
      121 ggcgatcgcg tcgtcggcgg cctcactgcg ccacgtggtg gcccaggtcg agcggctcgg
      181 gccggagccc ggtggtgcgc gcgtgaccct ctcgaacggt gagacgctcc cggctgacgt
      241 cgtcgtgctt gcgacgggcg tcgagacgcc ggccctgcct ccgtacctcg cggcgctcgt
      301 ggacgacgac cgggtcgtcg tggatccgtg ggtgcccgga gcgctcgacg gcatcgtgga
      361 tggcgggacc gtcgcgatca tcggcaccag cctcaccgcg gtcgacctcg cgggctcgat
      421 cctcaaccgg catccgaggg cacgggtgat cgcggtgtcg cgcaatggcc acctgccacg
      481 gccgcacgag gacccgtggc ggccccggtt ccccgagccg ccgttcacgg tcgccgagtt
      541 cctcgcgttc gaggacccat tcggcgaggc ggccgcgcgg ctgcgggcgt caggcgccga
      601 ctggccgcgt gccgtcgact cgctgcgccc gatctcgcag gcgctgtgga tggcgatggg
      661 cgacgacctg cggcgcacgt tcctcgacga ctaccgccat gactgggaga tccaccgcca
      721 ccgcatggcg gcggagatcg cgcgcgacct cgacgcgtgg acgacgcagg gccggttcga
      781 cgttcaagct gccgccatcg aggtggtcga gcccgcgggc acgcgcctgc ggatccgggc
      841 gctcgccgcc ggcgccgaca agccggcgtc gtgggaggtc gaccggatca tcgtcgccat
      901 cgggcccaac atggacccca tcgcgaatcc gctgctcggc gcggccatcg ccgacggaat
      961 cctgcgacct ggcccgctcg gcatcgggat cgacgtggac ccggcgacgg gcctcgtcat
     1021 cgacgcgaac ggcgacactc cacggcccgt gttcgcgatg ggcgcattgc gcaagggcgt
     1081 gttgtgggag accttggccg tgcccgagat ccgtggccag gcgcatgacg tcgcgtcgcg
     1141 gatcctgggc tcgaccgccg gctagccgga tccaccgacg gcatgggcga ttcgcctacg
     1201 ccgtggtctt cgtgccactc ccgtgcgcac aacgacacgt gtcttcgaga ttcggggcgc
     1261 ccgacgcgca gacgggcgtg attcccgtgc cgcgcgctcc ggaagcggcg cgggggtggc
     1321 gtaacggcga agccaggtgt ccttgtgcca ccccgggtgg cgtggcgggc cgccactcgt
     1381 ggtctcgctg acccgccact cggccggcgg ttagtgccag gcgttggggt tcgcgagcag
     1441 ccgctcgact gacaccggga acgtctcgct gaccccgtcc gcgagcgaga tcgactcgag
     1501 cacctcgcgc tcggcggccc ggacggcgat cagcgccgtc ttgaggccag ccgccgagcc
     1561 cgggtaggac agcttctcgg gtcgcgtgcc ggccacgttc accagggtcc cgtcgattgc
     1621 ccggatcacg tcagcgaacg agatcgcgga ggcggcgcga gcgagtgacc agccgccgtc
     1681 ggggccccgg cgcgccttca ccag
//
LOCUS       DBNHXO010000556         3225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3366886, whole genome shotgun sequence.
ACCESSION   DBNHXO010000556 DBNHXO010000000
VERSION     DBNHXO010000556.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3366886"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="AC162A_11190"
     CDS             complement(<1..614)
                     /locus_tag="AC162A_11190"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR5011451.1"
                     /translation="MIEGAVPREIREGPEAIRATVEVARADARRIARGWLAAGIDRVH
                     VIGNGTSFHSSLAAATLYRHRAGPDDPVVIPVTAAEFLLYAPRLGPRDAIVGISSSGE
                     FKDVVAVAEAVRGRFPMAGIVHVPGSTLTTVASDVVLSAGGPSTVPVMTKTVSSTLVA
                     TELLLVELLGQETADAEATRILAAADAAEAAIAAAEPLAEPFAES"
     gene            complement(611..1405)
                     /locus_tag="AC162A_11195"
     CDS             complement(611..1405)
                     /locus_tag="AC162A_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012669.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR5011452.1"
                     /translation="MLETERRTRLVEHLRADGRASVAELADDLGVTPSTIRRDLQRLA
                     REGRVVRTYGGAALSAATAGSTGSLRGDPQLDAKRAIARAAAARVADDSTIAIASGTT
                     TLAFAREIAHRRLTVITNALDIANVLLDRDGIELIVLGGVVRPRMHSMLGHLAELSMR
                     ELRADTLFMGIGAISLEQGLMNDSVPEILTDRALAQMSRSVVVLADATKFEQVAPGFV
                     FGLDEVDVLVTDDAAPAGTLDALRRRGIDVEAVPVPGRPERAEAGR"
     gene            1523..2491
                     /locus_tag="AC162A_11200"
     CDS             1523..2491
                     /locus_tag="AC162A_11200"
                     /inference="COORDINATES: protein motif:HMM:NF009498.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HIR5011453.1"
                     /translation="MAFQLGVLRGLDACSSPRGAFTVLALDHRQNLRKELRPDDPKAV
                     TEAEMVEFKQAVVRALGTTGTGILLDPEIGVGPAIADGSLPGGAGLIVAIEATGYEGP
                     SDARVSRVLPGWGVEQIKRLGASAAKLLVYYHPDAVNAADQERLVADVAAACRAADLA
                     LFLEPLSFGLDGSKLTGEERRRVVVETARRLTALGADVLKAEFPYDAGVSDESRWREA
                     CAELDAANPLPWVLLSGGVDDATFERQVRVACEAGASGVLVGRSVWAEAAALEGAARD
                     AYLMSTGRDRLGRLVELVDDLAAPWRPRWAAARRPEAPGPGWYERY"
     gene            2494..>3225
                     /locus_tag="AC162A_11205"
     CDS             2494..>3225
                     /locus_tag="AC162A_11205"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR5011454.1"
                     /translation="MTGRGGPGARDIDLLIVGEINPDIVVSDPDPRAAFGQEERFVES
                     VRLTVGSSCAITACGAARLGLAVSMVGVVGDDPLGRYMLEALAGRDVDVSACRVAPDR
                     PTGASVILSNGTDRATLTALGTIADVRLADVPPALLARVRHVHVGSYFLQPGLAAEVP
                     ALFRAAHAAGATTSLDTNWDPSGAWDGGFVAALAETDVLLPNAAEATRLARLDDVEAA
                     ALALASGPGGRRRTVAVKRGADGALA"
BASE COUNT          406 a         1144 c         1230 g          445 t
ORIGIN      
        1 gactcggcga atggctcggc gaggggctct gctgcggcga tcgcggcctc ggccgcgtcg
       61 gcggcggcga ggatccgggt ggcctccgcg tcagccgttt cctggcccag gagctcgacc
      121 agcaggagct ccgtcgcgac cagggtggac gagaccgtct tggtcatcac cggcacggtg
      181 gacggcccgc cggcggacag caccacgtcc gaggccacgg tcgtcagggt ggaaccgggc
      241 acgtggacga tccccgccat ggggaaccgg ccgcgcacgg cttccgcgac ggcgaccacg
      301 tccttgaact cgcccgacga cgagatgccg acgatggcat cgcgcgggcc gagtctcggc
      361 gcatagagga ggaactccgc cgcggtgacc gggatgacga cgggatcgtc cggtccggcg
      421 cggtggcggt acagggtggc cgcggccagg ctcgagtgga agctcgtacc gttgccgatg
      481 acgtggacgc ggtcgatgcc cgcagccagc cacccgcgag cgatccggcg ggcgtcggca
      541 cgggccacct cgacggttgc ccggatcgcc tccgggcctt cgcggatctc gcgcgggacc
      601 gcaccctcga tcatcggccg gcctcggcgc gttccgggcg accgggcacc ggcaccgcct
      661 cgacgtcgat cccccgccgc cgcagggcgt ccagcgtccc tgccggggcc gcgtcgtcgg
      721 tgacgaggac gtcgacctcg tccagcccga acacgaaccc cggggcgacc tgctcgaact
      781 tggtggcatc ggcgagcacc acgaccgagc gcgacatctg cgcgagtgcc cgatccgtca
      841 ggatctccgg gacggagtcg ttcatcagcc cctgctcgag gctgatcgcg ccgatgccca
      901 tgaacagcgt gtcggcgcgc agctcgcgca tcgacagctc ggccaggtgg cccagcatcg
      961 agtgcatccg gggccggacg acgccgccca ggacgatgag ctcgatgccg tcgcggtcga
     1021 gcagcacgtt cgcgatgtcg agggcgttgg tgatgaccgt gagccggcga tgggcgatct
     1081 cgcgcgcgaa cgcgagcgtg gtcgtgccgc tggcgatggc gatcgtcgag tcgtcggcca
     1141 ctcgcgcggc cgcggcgcgg gcgatggccc gcttggcgtc gagctgaggg tcgccgcgca
     1201 gcgagccggt ggatccggcg gtcgcggctg agagcgccgc gccgccgtag gtccggacca
     1261 cgcgtccctc gcgcgccagc cgctggaggt cgcggcggat cgtggacggg gtgacgccga
     1321 ggtcgtcggc gagctccgcc acgctcgcgc ggccgtcggc ccgcaggtgc tcgaccaggc
     1381 gcgtgcgacg ctccgtctcc agcatgcgcg gatcttgctc gtttctcgcg tagtttgtca
     1441 acgccgtgcg tgttctgcgc aggatgtgcg tttccatgcg cggaagttgc gcacgcggcc
     1501 accgggcgca tacgatccgg ccatggcctt ccagctgggc gtccttcgcg gcctggacgc
     1561 gtgcagctcg ccccgtggcg ccttcacggt gctggcgctc gaccaccgcc agaacctgcg
     1621 caaggagctg cgacccgacg accccaaggc cgtcaccgag gccgagatgg tcgaattcaa
     1681 gcaagccgtg gtgcgcgccc tcgggaccac ggggacgggc atcctgctcg acccggagat
     1741 cggcgttggc cccgcgatcg ccgacgggtc gctgcccggc ggggcaggcc tgatcgtcgc
     1801 catcgaggca acgggctacg aaggcccgag cgacgcacgc gtgagccggg tcctgcccgg
     1861 ctggggggtc gagcagatca agcgactggg cgcctcggcc gccaagctgc tcgtctacta
     1921 ccaccccgac gcggtcaacg cggccgacca ggagcgcctg gtcgccgacg tcgcggcggc
     1981 ctgccgcgcg gcggacctcg cgctgttcct ggaacccctc tcgttcggcc tcgatgggtc
     2041 gaagctcacc ggcgaggagc ggcgacgcgt ggtggtcgag accgcgcgac gcctgacggc
     2101 gctgggcgcg gacgtgctca aggcggagtt cccgtacgac gcgggtgttt cggatgagag
     2161 tcgatggcgc gaggcatgcg cggagctgga cgccgccaat ccgctcccct gggtgctctt
     2221 gtccgggggc gtggacgacg cgacgttcga gcgccaggtc cgggtcgcct gcgaggcggg
     2281 cgcgagcggc gtgctggtcg ggcggtccgt atgggccgag gcggccgcgc tcgagggggc
     2341 ggcacgcgat gcgtacctca tgtcgaccgg gcgcgatcgt ctcggccgcc tcgtcgagct
     2401 cgtggacgac ctcgccgcgc cgtggcggcc gcggtgggcg gcggcccgtc ggcccgaggc
     2461 gcccggaccc ggctggtacg agcggtacta gcgatgaccg ggcgcggcgg gccgggtgcg
     2521 cgcgacatcg acctgctcat cgtgggcgag atcaatcccg acatcgttgt ctcggacccc
     2581 gacccgcggg ctgcgttcgg gcaggaggag cgcttcgtgg agagcgtgcg gctgaccgtc
     2641 ggcagctcgt gcgcgatcac cgcgtgcggc gcggcgcgac tggggctggc ggtgagcatg
     2701 gtcggcgtcg tcggcgacga tccgctcggg cggtacatgc tcgaggcgct ggccgggcgg
     2761 gacgttgacg tctccgcctg ccgcgtagcg ccggatcgtc cgaccggcgc gtccgtgatc
     2821 ctcagcaacg ggaccgaccg cgcgacgctc accgcgctgg gcacgatcgc cgacgtgcgt
     2881 ctggccgacg ttccgccggc gctcctggcg cgcgtcaggc acgtccacgt cggcagctac
     2941 tttctccagc cgggtctcgc cgccgaggta cccgcgctgt tccgcgcggc tcatgcggcc
     3001 ggcgcgacga cgagcctgga caccaactgg gacccgtcgg gtgcctggga cggcgggttc
     3061 gtcgcggccc tcgccgagac cgacgtgctg ttgcccaacg ccgccgaggc cacgcggctg
     3121 gcccggctcg acgacgtgga ggccgccgcg ctcgcgctgg cctcgggccc cggtggccgt
     3181 cggcggaccg tggccgtcaa gcgcggcgcc gacggcgcgc tcgcg
//
LOCUS       DBNHXO010000557         3112 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_394895, whole genome shotgun sequence.
ACCESSION   DBNHXO010000557 DBNHXO010000000
VERSION     DBNHXO010000557.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_394895"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="AC162A_11210"
     CDS             complement(<1..408)
                     /locus_tag="AC162A_11210"
                     /inference="COORDINATES: protein motif:HMM:NF044585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6812 domain-containing protein"
                     /protein_id="HIR5011455.1"
                     /translation="MYSSFRDETPVELILYTDALMTRGLVRTSQHRVTDVLNHNDEAF
                     LILEDVTVEDLGNRGETTRVDYAQVNLDAVLFAVSNADVPSMPELRTPKEATEAIISV
                     PPFKIVGTIHLLPTQGNLRHGLTELTGRFLPVTG"
     gene            complement(467..2176)
                     /locus_tag="AC162A_11215"
     CDS             complement(467..2176)
                     /locus_tag="AC162A_11215"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HIR5011456.1"
                     /translation="MRRWTEIAERVAATTKTSEKTAILAGYLAGLSDAELPIAAVFLT
                     GRPFPEADQRSIGVGWSGLGGSVLRVAGADPEAMRRAYDRSSDVAIAVGEVLTEAGHA
                     PDPANEPTLPETQATFEAVEAAQGTAAKAALLDALLARSSPLTATGIVKVLSGELRIG
                     LREGLLEAAIAKAFDRPLDAVKRAGMLTGDVGRTALLAKADRLAEAALAMFHPLKFML
                     ASPAEDAAEIIGRLGPTVWVEDKYDGIRAQLHRSGGEVRLYSRDLHDISGQFPEVIAG
                     ARELRWAGILDGELLAWRDGVVLPFLQLQARLGRKNPSAKILAEIPVIFVAWDALGLD
                     TDGDGDPDGTVIPLLEEPLTERRRRLEALDLPLASEGGTFALSYLASVDSVDGLEEAF
                     AQARARRNEGLMVKDPDSRYSPGRRGLGWLKMKKALATLDCVVVGVEVGHGKRHGVLS
                     DYTFAVRDDETGALVTIGKAYSGLTDAEIAEMTRWFEAHTIATYGRYRQVEPTIVVEI
                     AFDVIMRSARHKSGFALRFPRIAQLRTDKPADEIDTLATVTRLYEGLQHGAEHLVTAG
                     ARR"
     gene            2203..2835
                     /locus_tag="AC162A_11220"
     CDS             2203..2835
                     /locus_tag="AC162A_11220"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5011457.1"
                     /translation="MLHRRWFPDHLDGDLVILRRHVRENLRDFMRWYQDPEVARLTRY
                     QDGPMRAEEVERFFTMRSLGPDSMAMGIHVRDTNRLIGSCAFSQLDGDNGSAMFHITI
                     GERDAWGHGYGSEATRLMVDHAFSTLGLHRVALSVFAFNERAIRAYLRVGFVTEGRAR
                     EAIWRDGRWWDELHMSVLEPEWRRTRWEERAIADGRSIPEREGVPVENTG"
     gene            2844..>3112
                     /locus_tag="AC162A_11225"
     CDS             2844..>3112
                     /locus_tag="AC162A_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011458.1"
                     /translation="MPSTGPAADAARAAFREQMDAKGHAVENARIAADGLDAAFTSGA
                     LVKTPALDGMLADLSLALEQDDGQKLGGKSAEAARFILRAISREL"
BASE COUNT          432 a         1068 c         1140 g          472 t
ORIGIN      
        1 cccggttacg ggcaggaatc ggccggtcag ctcggtcagg ccgtggcgga ggttgccctg
       61 tgtcggcagg aggtggatcg tgcccacgat cttgaacggc ggcaccgaga tgatcgcctc
      121 ggttgcctcc ttcggcgtcc ggagctccgg catgctcggc acgtcggcgt tcgagacggc
      181 gaacagcacg gcgtcgaggt tcacctgcgc gtagtcgacc cgcgtggtct cgccccggtt
      241 gccgaggtcc tcgacggtca cgtcctcgag gatcaggaac gcctcgtcgt tgtggttgag
      301 gacgtcggtg acccgatgct ggctggtgcg cacaagcccg cgcgtcatca gcgcgtccgt
      361 gtagaggatc agctcgaccg gggtctcgtc tctgaacgac gaatacacgt gtccctccat
      421 cctttcgtcc gcgcgcctgc ccgctcgcgc agcatacggg cccgcgctag cgacgcgcgc
      481 cggccgtgac gaggtgctcg gcgccgtgct ggaggccctc gtacaggcgg gtgacggtgg
      541 ccagggtgtc gatctcgtcg gcgggcttgt cggtgcgcag ctgcgcgatg cgtgggaacc
      601 ggagggcgaa gcccgacttg tggcgcgccg agcgcatgat cacgtcgaac gcgatctcca
      661 cgacgatcgt cggctcgacc tggcggtagc ggccgtaggt cgcgatggtg tgggcctcga
      721 accagcgggt catctcggcg atctcggcat cggtcaggcc gctgtaggcc ttgccgatgg
      781 tgacgagggc gcccgtctcg tcgtcgcgca cggcgaacgt gtagtccgac agcaccccgt
      841 ggcgcttgcc atggccgacc tcgaccccca ccaccacgca gtcgagcgtc gcgagcgcct
      901 tcttcatctt cagccagccg aggccgcgcc ggcccgggga gtaccggctg tccggatcct
      961 tgaccatcag cccctcgttg cgccgggcgc gggcctgcgc gaacgcctcc tcgagcccgt
     1021 ccaccgagtc gaccgatgcg aggtagctga gggcgaacgt gccgccctcg ctggcgagcg
     1081 ggaggtcgag cgcctccagg cggcgacggc gctcggtgag cggctcctcg agcagcggga
     1141 tcaccgtccc gtccgggtcc ccgtccccgt ccgtgtccag cccgagcgcg tcccaggcga
     1201 cgaagatcac ggggatctcc gcgaggatct tcgccgacgg gttcttgcgg ccgaggcgcg
     1261 cctggagctg caggaacggc agcacgacgc cgtcccgcca ggcgagcagc tcgccgtcga
     1321 ggatgcccgc ccaacgtagc tccctcgcgc cggcgatcac ctcggggaac tggccgctga
     1381 tgtcgtgcag gtcgcgcgag tacagccgga cctcgccgcc ggagcgatgc agctgggccc
     1441 ggatgccgtc gtacttgtcc tcgacccaga ccgtggggcc gagccggccg atgatctcgg
     1501 cggcgtcctc ggcgggcgac gcgagcatga acttgagggg gtgaaacatc gccagcgcgg
     1561 cctcggccag gcggtccgcc ttcgccagca gcgccgtgcg gccgacgtcg ccggtcagca
     1621 tgcccgcgcg cttgacggcg tcgagcgggc ggtcgaaggc tttcgcgatc gccgcctcca
     1681 gcaggccctc gcggaggccg atgcggagct cgccgctcag gaccttcacg atcccggtgg
     1741 cggtcagtgg ggaggatcgc gccagcaggg cgtcgaggag cgccgccttc gccgctgtgc
     1801 cctgggccgc ctcgaccgcc tcgaaggtgg cctgggtctc ggggagcgtc ggctcgttcg
     1861 ccgggtccgg ggcgtggccg gcttccgtca acacctcccc gacggcgatc gcgacgtcgg
     1921 acgagcggtc gtaggcgcgg cgcatggcct cggggtccgc cccggccacc cggagcaccg
     1981 atccgccgag acccgaccaa cccacgccga tcgaacgctg gtcggcctcg gggaacgggc
     2041 ggccggtcag gaagacggcc gcgatcggca gctcggcgtc ggaaaggccc gccaggtagc
     2101 ccgccaggat cgccgtcttc tccgaggtct tggtcgttgc ggcgacgcgc tcggcgatct
     2161 cggtccagcg gcgcatgatc gcgatggtat agggttcgag tgatgctcca tcgccggtgg
     2221 ttcccggatc acctagacgg tgatctcgtc atcctgcgca ggcacgttcg tgagaacctg
     2281 cgtgacttca tgcgctggta tcaggatccc gaggtcgccc ggctgacccg ctaccaggac
     2341 ggccccatgc gcgccgagga ggtcgagcgc ttcttcacga tgcgctcgct ggggcccgat
     2401 tccatggcca tggggattca cgtccgggac acgaaccggc tcatcgggtc gtgtgcgttc
     2461 tcgcagctcg acggcgacaa cgggtccgcg atgttccaca tcacgatcgg cgaacgcgat
     2521 gcctggggcc acgggtacgg cagcgaggcg acgcggctga tggtcgacca cgcgttctcg
     2581 acgctcgggc tccaccgcgt ggccctgtcg gtgttcgcgt tcaacgagcg ggcgatccgg
     2641 gcctacctgc gggtcgggtt cgtgaccgag ggccgtgccc gcgaggcgat ctggcgcgac
     2701 ggccgatggt gggacgagct tcacatgagc gtgctcgagc cggagtggcg ccggacgcgc
     2761 tgggaggagc gcgcgatcgc ggatgggcga tcgatccccg agcgcgaggg cgtcccggtc
     2821 gagaacacgg gctaggggcc cggatgccca gcacgggtcc cgcggcggat gcagcgcgcg
     2881 cggccttccg cgaacagatg gacgcgaagg gccacgcggt cgagaacgca cgcatcgcgg
     2941 cggacgggct ggacgccgcg ttcacgtcgg gagccctcgt gaagacgccg gcgctcgacg
     3001 ggatgctcgc ggacctgtcc ctcgcgctgg agcaggacga cggccagaag ctgggcggga
     3061 agtcggccga agccgccagg ttcatcctcc gcgcgatctc gcgcgagctg ga
//
LOCUS       DBNHXO010000558         2203 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5553777, whole genome shotgun sequence.
ACCESSION   DBNHXO010000558 DBNHXO010000000
VERSION     DBNHXO010000558.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5553777"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="AC162A_11230"
     CDS             <1..918
                     /locus_tag="AC162A_11230"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR5011459.1"
                     /translation="ANTSFAAFPRLAAVLAEDGFIPRQFAFRGDRLAFSTGIILLGTV
                     AGAVVIAGGASTHALIPLYAVGVFIDFTISQAGMVRHWIRTKDQGWRRRLTINAVGCT
                     LTAVIAVVVTSVKFIDGAWLVLLLIPALVGGMLFIRREYDGQSRELEVRDDLVFDKPH
                     REQRVVIPVNGINRSVIQAVMFGRSLATDPSMIQAMFVTTEPEEADALRERWERQLPG
                     IPLVIVESPYRALVGPVVTYLDVLDRAWPPDRPTPTTIVVLPEYVARHWWDRVLYNQT
                     AKRLKAALVGREHTVIADVPYRRSDHPHV"
     gene            1060..>2203
                     /locus_tag="AC162A_11235"
     CDS             1060..>2203
                     /locus_tag="AC162A_11235"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR5011460.1"
                     /translation="MVAKPAALGPQSGSERAVARVKVWLIGRPLASEEEIGERLPKRL
                     ALPIFSSDAISSSAYATEEIILAFILAGVAGAAFNLSVPVAIAIAALLGIVAFSYRQV
                     CIAYPTGGGSYSVSKANFGRMASLVAASALMIDYTLTVAVSTSSAVEQLSSAIPALDP
                     YRVEIAIVAIGLITLGNLRGLREAGNIFAIPTYLFLGSAFLMIAAGFIRIVFQGDTGP
                     APTPEVIAATTETAKGVTVLILLRAFAAGAVALTGTEAIATGVPAFKPPEARNAARTL
                     MVMAGILAVLFVGITFLATSFHIMPIEEPKTTVIAQVARHVYGDSSVGFYLFQAFTAL
                     LLFLAANTSFAAFPRLAAVLAEDGFIPRQFAFRGDRLAFSTGIILLG"
BASE COUNT          290 a          818 c          729 g          366 t
ORIGIN      
        1 gcgaacacct cgttcgccgc gtttccgcgc ctcgcggccg tgctggccga ggacggcttc
       61 atcccgcgcc agttcgcgtt ccggggcgac cgcctcgcgt tctcgacggg gatcatcctg
      121 ctcgggaccg tcgccggcgc ggtggtcatc gccggcggcg cctcgaccca cgcgctgatc
      181 ccgctgtatg cggtcggcgt gttcatcgac ttcacgatct cgcaggcggg catggtccgc
      241 cactggatcc ggaccaagga ccagggctgg cgccgtcgcc tcacgatcaa cgcggtcggc
      301 tgcacgctca cggccgtcat cgcggtggtc gtcacgtcgg tcaagttcat cgacggcgcc
      361 tggctcgtcc tgctgctgat cccggcgctg gtgggcggga tgctgttcat ccggcgcgag
      421 tacgacggcc agtcccgcga gctcgaggtc cgcgacgacc tcgtgttcga caagccgcac
      481 cgcgagcagc gcgtcgtgat cccggtcaac ggcatcaacc gctcggtgat ccaggccgtc
      541 atgttcgggc gttcgctggc gaccgacccc tcgatgatcc aggcgatgtt cgtgaccacg
      601 gaacccgagg aggccgacgc gctgcgcgag cgctgggagc ggcagctgcc ggggatcccg
      661 ctggtcatcg tcgagtcgcc ctaccgcgcc ctggtcggac cggtcgtgac ctatctcgac
      721 gtcctcgacc gcgcctggcc gccggaccgc ccgacgccca ccacgatcgt cgtgctgccc
      781 gagtacgtcg cgcggcactg gtgggatcgc gtcctgtaca accagacggc aaagcgcctc
      841 aaggccgcgc tcgtcggccg cgagcacacg gtgatcgcgg acgtgccgta tcgtcggagc
      901 gatcacccgc acgtctagcg catcgctcgc attgaggtcg gtccaaccca gcccatgatc
      961 ggtggacggc ggcccatcaa gggacggaag cccgcggacc ggcgcgtccg cgtcgagcgt
     1021 ccacactccg cctacttccg gtacacgggg ccgggacaga tggtggccaa gcccgccgcg
     1081 ctgggcccgc agtcgggcag cgagcgggcc gtcgcccggg tcaaggtgtg gctcatcggc
     1141 cggcccctcg cgagcgagga ggagatcggc gagcggctcc ccaagcggct cgccctgccg
     1201 atcttcagct cggacgccat ctcgtcgtcg gcctacgcga ccgaggagat catcctcgcg
     1261 ttcatcctcg ccggggtcgc gggcgcggcg ttcaacctct cggtcccggt cgccatcgcg
     1321 atcgccgccc tgctgggcat cgtcgccttc agctaccgcc aggtctgcat cgcctacccg
     1381 accggcggcg gctcgtactc ggtgtccaag gcgaacttcg gccgcatggc atccctcgtg
     1441 gccgcctcgg cgctgatgat cgactacacg ctgacggtcg ccgtgtcgac gtcgtcggcc
     1501 gtcgagcagc tctcgtcggc gatcccggcg ctcgacccat accgggtgga gatcgcgatc
     1561 gtcgccatcg gcctgatcac gctgggcaac ctgcgcgggc tgcgcgaggc cggcaacatc
     1621 ttcgccatcc cgacctacct gttcctgggc tcggcgttcc tgatgatcgc cgccgggttc
     1681 atccggatcg tgttccaggg cgacaccggt ccggcgccca cgcccgaggt catcgccgcg
     1741 accaccgaga ccgcgaaggg cgtgaccgtc ctgatcctgc tgcgcgcgtt cgccgccggc
     1801 gcggtcgcgc tcaccggcac cgaggcgatc gccaccggcg tgcccgcgtt caagccgccc
     1861 gaggcgcgca acgccgcccg gacgctgatg gtgatggccg gaatcctcgc ggtcctgttc
     1921 gtggggatca cgttcctcgc cacgagcttc cacatcatgc cgatcgagga gccgaagacg
     1981 acggtgattg cccaggtcgc ccgccacgtg tatggcgaca gcagcgtcgg cttctacctg
     2041 ttccaggcgt tcaccgctct gctgctgttc ctcgccgcga acacctcgtt cgccgcgttt
     2101 ccgcgcctcg cggccgtgct ggccgaggac ggcttcatcc cgcgccagtt cgcgttccgg
     2161 ggcgaccgcc tcgcgttctc gacggggatc atcctgctcg gga
//
LOCUS       DBNHXO010000559         4842 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4758454, whole genome shotgun sequence.
ACCESSION   DBNHXO010000559 DBNHXO010000000
VERSION     DBNHXO010000559.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4758454"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="AC162A_11240"
     CDS             <1..567
                     /locus_tag="AC162A_11240"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR5011461.1"
                     /translation="VDRLAAAAAFTHRLLDPLAAAAGAQRWIEMTPGNVYVAPTLLRM
                     FPDMRLVHSVRDGRDVACSVAPLAWGPDDLDDALDWWAAGLERASKATEAVPPDRLLV
                     IRMEDLVANDREASYGRLLAFAGLEDNPAMRSYFEDRATAERAHIGRWESDVPPDRRA
                     VFDAHYRSLEARLSAVVPELSTSGAPGR"
     gene            670..1083
                     /locus_tag="AC162A_11245"
     CDS             670..1083
                     /locus_tag="AC162A_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011462.1"
                     /translation="MKFILGLLTGIVLGAIGAVLYSVTTGRDLREEYAGVRDEVMQRD
                     FDALSKRLEDRANELQSTLQDRVSQIQGSHPGTNGNGSAGDAIADAGEAAGDAIKDAG
                     DAAGKAVDDAARKAKGVAKDAGAAVDDAASELPSA"
     gene            1204..3084
                     /locus_tag="AC162A_11250"
     CDS             1204..3084
                     /locus_tag="AC162A_11250"
                     /inference="COORDINATES: protein motif:HMM:NF017369.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011463.1"
                     /translation="MRRPTLPAVIALLLIGALVAPAGAAAAGPSNDETVTGVLQAYYV
                     DDFDSRGDDDTLHYDLRTASGTIPVRFPGRGPVRLVGSKVTLTGTLRGGALRVASSSP
                     GADLRVRAAAGHIDADSDALTFAVVLMNFTDIPTQPWTKATVASAIAGGSSSMKAFFE
                     EESKGDVSVSASVYGWYTIPATSAGCDWQDWETLGWDAATAAGVDLTAFTNVMFISPY
                     TSSCGFAGVGYVPGPYTYLNGTISTQVMAHEVGHNLGLAHANARTCTVDGTRVTIAAN
                     SACSTQGYADPFSVMGNVALRHDHGSQLGELGWLSASQKVVGSPGHTYTIAPYFGSGT
                     VKLVRIPRGDGSYFDLDLRTPHGVFDSFSAGSPVVNGVTVRLGWGSASPTWSPVATEL
                     LDTTPATASLNDASLLVGQTMTDPVSTISITTQSIGASGVTVEVSESTPPGMPGSLAA
                     TLYSTPSVGLAWTAASDNAGIESYRVRRDGATVATLGAGARDWLDQDVELGSSYDYSV
                     AAIDTSGNVGPAATISIDVEPGGGPPPPDTEAPGKPGNFHVRHRQHGGPHVTFAWAAA
                     TDNVGVVKYKIYRAGHARPIATTTSRHIRITTKAGAKYKVRAFDAAGNRSAASNKVRG
                     RR"
     gene            complement(3417..4070)
                     /locus_tag="AC162A_11255"
     CDS             complement(3417..4070)
                     /locus_tag="AC162A_11255"
                     /inference="COORDINATES: protein motif:HMM:NF025120.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5011464.1"
                     /translation="MPRRPKVVERGHVRGVELTALLQRELRDARHDRGLSQASVATAL
                     GVDRSWVSRVERGRVDDVGIIAATELLAAVGLELSVRAYPSGGPLRDAAHSALLEHLR
                     ARIHRSLSWATEVPLPMVGDMRAWDALIRGRDWRCGVEAETRPRDLQALERRLALKLR
                     DGGVDMLVLLLLDSRHNRALVRENADALHERFPVPGLRAIELLGAGVSPGGSSLILL"
     gene            4134..4469
                     /locus_tag="AC162A_11260"
     CDS             4134..4469
                     /locus_tag="AC162A_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011465.1"
                     /translation="MPTQAWALVALFSLCSIVLGVVAVRIAARGSGPVGLAAYVLPVV
                     AAFGAFYVIGHRLGLAVGPEIGLFGFQVALIGDLAIGFAAALAVALAQAAVVRARRRG
                     SAGVEGERG"
     gene            complement(4466..>4842)
                     /locus_tag="AC162A_11265"
     CDS             complement(4466..>4842)
                     /locus_tag="AC162A_11265"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010549987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR5011466.1"
                     /translation="LAALEERLGSVGVIDARAPERYRGEVEPVDGVPGHIPTAVNRPS
                     GGNLGPDGLFLPPEALRDRFEALTAVGDVVSYCGSGVTACHNALAMRIAELPDPLVYA
                     GSYSDWTRAGRPVATGSEPGPA"
BASE COUNT          699 a         1737 c         1735 g          671 t
ORIGIN      
        1 gtggaccggc tggctgcagc ggccgcgttc acgcaccgcc tgctcgatcc tctggcggcc
       61 gcggcgggcg cccagcgctg gatcgagatg acgccaggga acgtctacgt ggcgccgacc
      121 ctcctgcgca tgttcccgga catgcgtctg gtgcacagcg ttcgcgacgg gcgcgacgtc
      181 gcgtgctcgg tcgctcccct cgcctggggc ccggacgacc tggacgacgc gctcgactgg
      241 tgggccgccg gcctcgaacg cgcttcgaag gcgacggaag ccgtgccgcc ggaccgcctc
      301 ctggtcatac gcatggagga tctcgtcgcg aacgaccgcg aggcatccta cggacgactg
      361 ctcgccttcg cgggtctcga ggacaacccg gcgatgcggt cgtacttcga ggaccgggcg
      421 acggccgaac gggcgcacat cgggcggtgg gagagcgacg tgcctccgga tcggcgagcg
      481 gtgttcgacg cccactaccg atcgctggag gcgcgcctgt ccgccgtggt acccgagctg
      541 tccacaagcg gggcaccggg tcggtagcga ccgggtcagg gccggagaac cgtgcggccg
      601 gagtaccatc atcgtcccac gcgagggatg cgccggcgtt ggtccggccg acaaccggga
      661 gagttggcca tgaaattcat cctcggactg ctgacaggga tcgtcctcgg ggcgatcggc
      721 gccgtcctgt actccgtgac gacggggcgc gacctgcgcg aggagtacgc gggtgtccgc
      781 gacgaggtca tgcagcgcga cttcgacgcg ctgagcaagc gcctcgagga ccgggccaac
      841 gagctgcagt cgacgctcca ggatcgggtg agccagatcc aggggtcgca cccgggcacg
      901 aacggaaacg gcagcgcggg cgatgccatc gccgacgcgg gcgaggccgc gggcgacgcg
      961 atcaaggacg cgggcgacgc cgccggcaag gcggtcgacg atgccgcccg gaaggcaaag
     1021 ggcgtggcga aggacgcggg cgcggccgtc gacgacgcgg cctcggagct gcccagcgcc
     1081 tgaggcgccg ctcctcgtca ccgatcgaag cggtgcgtag acgcaccgaa cgggaccatc
     1141 gcgtcgcggg cgcgcgacgc acgggtgatg cgcgccgggc gcgtcttggc cgaggctccg
     1201 gacatgcgcc gtcccaccct gcccgccgtt atcgcgctgt tgctgatcgg tgccctcgtc
     1261 gcgcccgccg gcgcggccgc ggcggggccg tccaacgacg agaccgtgac gggcgtgctc
     1321 caagcgtatt acgtcgacga cttcgacagt cgcggtgacg acgacaccct tcactacgac
     1381 ctgcggaccg ccagcggcac gatcccggtc cggtttcccg gccgcggacc cgtccggctg
     1441 gtcggctcga aggtcacgct gactgggacg cttcggggtg gcgcgctccg cgtcgccagc
     1501 tcgtcgcccg gcgccgacct gcgtgtccgc gcggcggccg gccacatcga cgccgattcg
     1561 gacgcgctca cgttcgccgt cgtcctcatg aacttcaccg acatcccgac ccagccctgg
     1621 accaaggcaa ccgtcgcgag cgccatcgcc ggcggttcat cgagcatgaa ggcgttcttc
     1681 gaggaggagt cgaagggcga cgtgagcgtc agcgcctcgg tctacggctg gtacacgatc
     1741 ccggcgacat cggcggggtg cgattggcag gactgggaaa cgctgggctg ggacgccgcg
     1801 acggcggccg gggtcgacct cacggcgttc accaacgtga tgttcatctc gccctacacg
     1861 agcagctgcg ggttcgcggg cgtcggctat gtgcccggcc cgtacacgta tctcaacggg
     1921 acgatcagca cgcaggtcat ggcccacgag gtcggccaca acctggggct cgcgcacgcg
     1981 aacgcgcgga cctgcacagt cgacgggacg cgggtcacca tcgccgcgaa cagcgcctgc
     2041 tcgacgcagg gctacgccga cccgttctcc gtgatgggca acgttgcgct gcgccacgac
     2101 cacggctcgc agctgggcga gctcggctgg ctgtccgcct cgcagaaggt ggtgggatcg
     2161 ccggggcaca cgtatacgat cgcgccgtac ttcggatcgg ggacggtcaa gctcgtccgg
     2221 atcccgcgcg gcgacggcag ctacttcgac ctcgacctgc ggacgccgca cggggtcttc
     2281 gactcgttct cggcgggctc gcccgtcgtg aacggcgtca cggtccgcct gggctggggc
     2341 tcggccagcc cgacctggtc gccggtcgcc acggagctgc tcgacacgac gcccgcgacg
     2401 gcgtcgctca acgacgcctc gctgctcgtg ggccagacga tgaccgaccc ggtctcgacg
     2461 atctcgatca cgacgcagtc gatcggcgcg tccggcgtca cggtcgaggt gtccgagtcg
     2521 acgccgccgg ggatgccggg gtcactcgcc gcgacgctgt actcgacgcc gagcgtgggt
     2581 ctcgcgtgga ccgccgcgag cgacaacgcg ggcatcgagt cgtaccgggt ccgccgggac
     2641 ggggccaccg tggcgacgct gggggcgggc gcgcgcgact ggctcgatca ggacgtcgag
     2701 ctcgggtcct cgtacgacta ctcggtggcc gcgatcgaca cctcgggcaa cgtcggcccc
     2761 gcggcgacga tctcgatcga tgtcgagccg ggcggtgggc cgccgccacc cgacacggag
     2821 gcaccgggca agccgggcaa cttccacgtc cgccaccgcc agcacggcgg gccgcacgtc
     2881 acattcgcct gggccgccgc gacggacaac gtcggcgtgg tcaagtacaa gatctatcgc
     2941 gccgggcacg cccggccgat cgcgacgacg acgtcgcgcc acatccggat cacgaccaag
     3001 gccggcgcga agtacaaggt ccgggcgttc gatgccgccg gcaaccgcag cgcggcctcg
     3061 aacaaggtcc gcggccgccg ctagcccgtt cgccgccgcg cctgcggctg tttcggcggc
     3121 tgagcccggc cgtccgcgcc cccgcggggt gccccagccc ggccctccgc gcccttgcgc
     3181 tcgatctgtg caccccgtgc acatcgcccg atgggttgtg cagcagaggc acattcttgc
     3241 cctccgctgc gcgtgtcacg tcgattctgt gcgtgtacat cacgacgaca gcgccggctg
     3301 accgccgggt cgacacgcga gcagcacatg tgcgccagga gcacaagtgc gacgcgaccg
     3361 gccatcggat cgagcccgac cgcgcaggaa tgtgcaccag ctgcacaccg acgagatcag
     3421 agcaggatca gcgagctccc gccgggcgag acacccgcgc caagcagctc gatggcgcgc
     3481 aggcccggaa ccgggaagcg ctcatggagg gcgtcggcgt tctcgcggac gagcgcgcgg
     3541 ttgtgccggc tgtcgaggag cagcaggacg agcatgtcca cgccaccgtc gcgcagcttc
     3601 agggccagtc ggcgctcgag cgcctggagg tctcgtggcc gggtctccgc ctcgacgccg
     3661 cagcgccagt cgcggccacg gatcagcgcg tcccacgccc gcatgtcgcc caccatgggg
     3721 agggggactt ccgtcgccca gctcagactc cggtggatcc gcgcgcgcag gtgctcgagc
     3781 agcgccgagt gcgcggcatc gcgcagcgga cctcctgacg ggtaggccct gaccgacagc
     3841 tcgagcccga ccgccgcgag cagctcagtc gccgcgatga tgcccacgtc atcgactcgg
     3901 ccgcgttcga cgcgactgac ccacgagcgg tcgacgccga gggcagtcgc cacgcttgcc
     3961 tggctgagtc cgcggtcgtg tcgagcgtcc cggagctcgc gctgcaagag ggcggtcagc
     4021 tcaacgcctc gcacgtgccc tcgctcgacg accttaggac gcctcggcat cgccacagca
     4081 tgaaaacggc gacttcacca cggcttatca cggccggccg gtagattggg gtcatgccga
     4141 cccaagcctg ggcgctcgtc gccctgttca gcctgtgttc gatcgtcctg ggggtcgtgg
     4201 cggtgcggat cgccgctcgc ggcagcggac ccgtaggcct cgccgcgtac gtcctgcccg
     4261 ttgtcgccgc attcggcgcg ttctacgtca tcggccaccg gctggggctc gcggtgggtc
     4321 cggaaatcgg cctgttcggg ttccaggtcg cgctgatcgg cgacctcgcg atcgggttcg
     4381 cggctgcgct ggccgtcgcc ctggcccagg cggcggtcgt tcgggcaagg cggcgagggt
     4441 ccgcgggcgt cgagggcgag cgaggctagg ccggccccgg ttcgctgcct gtcgccaccg
     4501 ggcggcccgc ccgcgtccaa tcactgtacg accccgcata gaccaggggg tcggggagct
     4561 cggcgatccg catcgccagc gcgttgtggc acgccgtgac gccgctcccg cagtagctga
     4621 cgacgtcgcc cacggcggtc agggcctcga atcggtcccg gagtgcttcg gggggcaaga
     4681 agaggccgtc cggcccgagg ttgccgccgc tcggccggtt gaccgctgtc gggatgtggc
     4741 cgggcacgcc gtcgacgggc tcgacctcgc cgcggtatcg ctcgggcgcc cgcgcgtcga
     4801 tgacgccaac gctccccagc cgctcctcga gtgccgccag at
//
LOCUS       DBNHXO010000560         3242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3369164, whole genome shotgun sequence.
ACCESSION   DBNHXO010000560 DBNHXO010000000
VERSION     DBNHXO010000560.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3369164"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="AC162A_11270"
     CDS             complement(<1..854)
                     /locus_tag="AC162A_11270"
                     /inference="COORDINATES: protein motif:HMM:NF015736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HIR5011467.1"
                     /translation="MTTETGEAAKATWRLEGPNGSMAPRSAIEVLVDLNDKVTTGKVG
                     EYQPVPLGFTPLDKTIGTGLRAGELLLIGGAQGTGKTTMALQMARNVASGGQANVLYI
                     CFEHDELYLLNRLIAAESALAHLPHKTGAIKIQDVRKEILGTWMAEGGGAAQLAANPR
                     LRPSLDRIARYGQNLFLLRGSQTASTIDGIRELINQHRELSGDRRLMVVVDYLQKVPV
                     IPEPLTESEKVTFVVNGLKDIALSMDVPMIAIVAADKEGLKASRLRNYHLRGSSAINY
                     EADIILIL"
     gene            complement(973..2400)
                     /locus_tag="AC162A_11275"
     CDS             complement(973..2400)
                     /locus_tag="AC162A_11275"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR5011468.1"
                     /translation="MTEPSPAPVAGPIDPAIEAALQASLDARIESLLEIARIPSVSSL
                     HEHSADMVATADWIADRLRGLGATEVEVIATPLHPIVYGRIHEAPGAPTAIVYCHYDV
                     QPVDPLDLWEHPPFEPFVRDGRFIGRGVADDKGQLVMHLAALEALRSVGRAPAVNLTF
                     VFEGEEEFGSRSLEAWIPDNRDRLQADAVIISDTGFFAGNLPAITTSLRGIMYAQIDV
                     VLSPVDLHSGGYGGVVQNPANALASIIAALKGPDGRVRIPGFYDDVVPLTADERAAMA
                     ALPFDDETYRAEIPVSELVGEAGWTTLERRATRPTLDVNGIWGGFQGEGAKTIIPAHA
                     HAKVSTRLVSDQDPVRVYEALRDHVAAVAPPGVTVTTNYVHGGRPSLTPSDHPATLAA
                     SRALRAVYDVDPVLIREGGSIPVTAVFEHDLGLPVVLLGFAQPACNAHAPNEWFLVEN
                     FEQGTRVIVRLWDELAAAFRATEPA"
     gene            complement(2530..>3242)
                     /locus_tag="AC162A_11280"
     CDS             complement(2530..>3242)
                     /locus_tag="AC162A_11280"
                     /inference="COORDINATES: protein motif:HMM:NF013593.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR5011469.1"
                     /translation="ERKSRVISDEEKRIIAYHEGGHAVVQRILPKCDPVAKVTVISRG
                     MALGYTMALPTEDRYLQSKSEFEDKIAGLLGGNVAERIVFGDTTTGASNDIEKATDLA
                     RRMVTEFGMSDRLGPLSFGKRDELVFLGREIGEQRNYSDDVAKQIDEEVRAIIDRAYE
                     RATETLTAHRDRLDRLAEKLIAEETVDTEGFEQLFDDLPPKPTRQAGIPRVIAPGTDG
                     GTTSGPAAPAPNPSPQPA"
BASE COUNT          440 a         1105 c         1166 g          531 t
ORIGIN      
        1 ttcaggatca ggatgatgtc ggcctcgtag ttgatcgccg acgacccgcg caggtggtag
       61 ttgcgcaggc gcgacgcctt gagaccttcc ttgtccgcgg cgacgatggc gatcatcggc
      121 acgtccatcg agagcgcgat gtccttgagg ccgttcacga cgaacgtgac cttctcgctc
      181 tcggtcaacg gctccgggat caccggcacc ttctggaggt agtcgacgac gaccatcagc
      241 cggcggtcgc ccgagagctc gcggtgctgg ttgatcagct cgcggatgcc gtcgatggtg
      301 ctcgcggtct ggctgcctcg cagcaggaac aggttctgcc cataacgggc gatccggtcc
      361 agcgacgggc gcagacgcgg attggccgcg agctgggcgg caccgccgcc ctcggccatc
      421 caggtgccca ggatctcctt gcggacatcc tggatcttga tcgcgccggt cttgtgcggc
      481 aggtgggcga gcgccgactc tgcggcgatc agccggttga gcaggtagag ctcgtcgtgc
      541 tcgaagcaga tatagagcac gttggcctgg ccgccggacg ccacattgcg cgccatctgg
      601 agggccatcg tcgtcttgcc ggtgccctgg gcaccgccga tgagcaggag ctcacccgcg
      661 cgcaggcccg tgccgatggt cttgtcgagc ggtgtgaacc cgagggggac gggctggtac
      721 tcgccgacct tgccggtggt caccttgtcg ttgaggtcga ccagcacctc gatcgccgac
      781 cgaggcgcca tgctcccgtt cgggccttcg agacgccagg tggccttcgc cgcttccccg
      841 gtctccgtcg tcacgtgctt tgtgttcctc cagcgatagc cgtgagttgc atcctagcac
      901 cgggtttttg gcgcacagtg acccgacacg gtgcgcccga tgggccgaag gtacggggtg
      961 cgcgcacgca cctcaggccg gttcggtcgc ccgaaacgcc gctgccagct cgtcccacag
     1021 acggacgatg acgcgggtgc cctgctcgaa gttctcgacc aggaaccact cgttgggagc
     1081 gtgggcgttg caggccggct gcgcgaagcc gagcaggacg acgggcagcc cgagatcgtg
     1141 ctcgaagacc gcggtcaccg ggatggagcc gccctcccgg atcagtacgg gatcgacgtc
     1201 gtagaccgcg cgcagcgccc gcgacgccgc cagcgtcgcc gggtggtccg aaggagtgag
     1261 gctggggcgg ccgccgtgga cgtagttggt cgtgacggtg accccgggcg gcgcgacggc
     1321 ggcgacatgg tcgcgcaggg cctcgtatac gcgaaccggg tcctggtcgg agacgagcct
     1381 ggtcgacacc ttggcgtgcg cgtgggccgg gatgatcgtc ttcgcgcctt caccctggaa
     1441 cccgccccag atgccgttca cgtcgagcgt cgggcgcgtg gcccgccgtt cgagcgtcgt
     1501 ccagccggcc tcgccgacca gctccgacac ggggatctcc gcgcggtacg tctcgtcgtc
     1561 gaacggcagc gcggccatcg cggcgcgctc gtccgccgtg agcgggacga cgtcgtcgta
     1621 gaagcccggg atccggacgc gaccgtccgg gcccttgagc gcggcgatga tcgaagcgag
     1681 ggcgttggcc gggttctgga ccacgccgcc gtagccgccc gagtggaggt cgaccggcga
     1741 cagcaccacg tcgatctggg catacatgat gccgcgcagg ctggtcgtga tcgccggcag
     1801 gttgcccgcg aagaaccccg tgtcgctgat gatcacggcg tccgcctgga gccggtcgcg
     1861 gttgtcgggg atccacgcct ccaagctgcg cgacccgaac tcctcctcgc cctcgaacac
     1921 gaacgtcagg ttcaccgcgg gtgcccgtcc gaccgaccgc agcgcctcga gggcggccag
     1981 gtgcatgacc aactgcccct tgtcgtccgc tacgccgcgg ccgatgaacc ggccgtcgcg
     2041 aacgaacggc tcgaacggcg ggtgctccca caggtccagg gggtcgacgg gctggacgtc
     2101 gtagtggcag tacacgatcg ccgtgggcgc accgggagcc tcgtggatcc gaccgtagac
     2161 gatcgggtgc agcggcgtcg cgatcacctc gacctcggtc gccccgagcc cacgcaggcg
     2221 gtccgcgatc cagtccgccg tcgcgaccat gtcggcgctg tgctcgtgga gcgacgacac
     2281 gctcgggatc cgcgcgatct cgaggagcga ctcgatccgc gcgtcgagcg acgcctgcag
     2341 cgccgcctcg atggccggat cgatggggcc ggcgacgggc gcgggactgg gctcggtcat
     2401 cgtggctcct gcggcacggc gggacgcgac cgatcgtagc gcgctcgcgc gcagcgccgg
     2461 gtggcccgtt aacgcgcaga gggcggaccg tgtggtccgc cctccgtggg tgtcgagtgg
     2521 gtcgccgggt caggccggct ggggcgaggg gttgggcgcc ggggccgccg ggccgctggt
     2581 ggtgccgccg tccgtgccgg gcgcgatcac gcgggggatc cccgcctggc gcgtcggctt
     2641 gggcggcagg tcgtcgaaca gctgctcgaa gccctccgtg tcgacggtct cctcggcgat
     2701 cagcttctcc gccaggcggt cgagccggtc gcggtgggcc gtcagggtct cggtcgcccg
     2761 ctcgtaggcg cggtcgatga tcgcccgcac ctcctcgtcg atctgcttcg cgacgtcgtc
     2821 cgagtagttg cgctgctcgc cgatctcgcg gccgaggaac acgagctcgt cgcgcttccc
     2881 gaacgagagc ggacccagcc ggtcgctcat gccgaactcg gtcaccatgc ggcgcgcgag
     2941 gtcggtcgcc ttctcgatgt cgttgctggc gcccgtggtc gtatcaccga acacgatccg
     3001 ctcggcgacg ttgccgccga gcaggcccgc gatcttgtcc tcgaactcgg acttgctctg
     3061 caggtagcgg tcctcggtgg gcagggccat cgtgtagccg agcgccatgc cgcggctgat
     3121 gaccgtgacc ttggcgaccg gatcgcactt gggcaggatc cgctgcacga cggcgtggcc
     3181 gccctcgtgg taggcgatga tccgcttctc ctcgtcgctg atgacgcggc tcttgcgctc
     3241 cg
//
LOCUS       DBNHXO010000561         4485 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5156833, whole genome shotgun sequence.
ACCESSION   DBNHXO010000561 DBNHXO010000000
VERSION     DBNHXO010000561.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5156833"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="AC162A_11285"
     CDS             <1..267
                     /locus_tag="AC162A_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017249558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR5011470.1"
                     /translation="GSVFRNPDGDSAGRLIDELGLKGYRIGGASVSEKHANFIVNDQR
                     GSAADVRRLAEHVRSEVLARHGTELAFEVEFAGDWSGWPPEEAA"
     gene            267..1409
                     /locus_tag="AC162A_11290"
     CDS             267..1409
                     /locus_tag="AC162A_11290"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR5011471.1"
                     /translation="MSVPPLAHDPAATPVAVVLGGPSAEHDVSIVSGTAILEALGGSG
                     YPAEAWLVDLGGGWWRLPDGHRRDGRPQALYDDPAAVGGTGPLHAAAALDGLAAQARP
                     PVVFLALHGPFGEDGTVQALCEAAGLAYTGSGVTASAIGMDKAVFKRLIRGLGLPVVD
                     WREVRAARWSRDPGSVLAELEAFAAGTGDARLMVKPARLGSSVGMTLAHTVGERAGAL
                     ETAFRYDDLVLVERYLAGARDLEVAVMGNEPDALELYGPGEILAGHEFYDYAAKYTPG
                     LSESTTSAEVTPAERAATLKLARDAYRATGCEGFARVDFLLAGPELVVSEINTIPGFT
                     PISLFPTLPAAGGYDFAAVCSRVVDLALERAAGRVRHRLTAADLPR"
     gene            1412..2266
                     /locus_tag="AC162A_11295"
     CDS             1412..2266
                     /locus_tag="AC162A_11295"
                     /inference="COORDINATES: protein motif:HMM:NF020067.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR5011472.1"
                     /translation="MQRRGAASRQGAGVRRTRTVRRRSAGLTPTRAGALLALLLSLAG
                     LYGATSSGAFSMRRLDVSGVTWTSEDAVLAALAIPDGQNLFTLSTGDLIDRLTVLPAV
                     RDASITIALPDEVRVDVAERVALLAWEVDGQRYLVDEDGLLFARVDETTPAAADRLPF
                     VDDHRVAAGALTVGATLDPVILDAARRLGSLRPADLGTSAASLAIRLDDENGFTLRAE
                     PVGWSAVFGFYTPTLRTTDLVPGQVRLLRSMLAGREDRILKVILADDRSGTMIPREDA
                     SPSGEPAP"
     gene            2346..2669
                     /locus_tag="AC162A_11300"
     CDS             2346..2669
                     /locus_tag="AC162A_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HIR5011473.1"
                     /translation="MLLPLVGLLVGVVAGLILNVNVSFEFSRYSAVAILAALDSVLGA
                     VRAELDGVYDNRIFISGFVVNAIVAVLLVFIGDKLSLDLYLVALINFGFRIFNNVALI
                     RRHFI"
     gene            2694..3923
                     /gene="ftsA"
                     /locus_tag="AC162A_11305"
     CDS             2694..3923
                     /gene="ftsA"
                     /locus_tag="AC162A_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR5011474.1"
                     /translation="MEREAVLVGIDVGTSKVCALIGEVARDGRLTIMGHGTVPASGLK
                     KGVVVNIDQTVRSIADAVERAERLSGWKIDRAFVGVGGNHVESLNSTGQVAVTAHHRE
                     VTREDISRAIEVARAVSIPNNRDVLHVERRGFTVDGQEGVKDPLGMSALRLEVEAHIV
                     SASATAVQNLTKCVQLAGVKIDELVADALASSEAVLTETEKELGVAVADIGAGTIDLS
                     LFADGSPFHTSVLPVGGNNVTNDVAIGVKTTLQVAEELKVRHGTCDLRGLEDEEIAVS
                     MVGDDAGRTVSREELCQIIEARMRETFELLRGEMTRSGHGMLPAGIILTGGAAQLAGT
                     AELGREVLQMPVRVGSPSGIAGLVDTIQDPSYSTAVGLLRWGAIQVAQGEPLRYESAP
                     GGGGFGRIRDAIRSIFP"
     gene            4186..>4485
                     /locus_tag="AC162A_11310"
     CDS             4186..>4485
                     /locus_tag="AC162A_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017545088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HIR5011475.1"
                     /translation="MQSIQLEVRTGRDRGFTDLTPACSRFAAEASQGGDGLLSLFVPH
                     ATAGVVILELGAGSDVDAVATLDRLLPRDDRWTHRHGSPGHGADHVLPLLAAPSIT"
BASE COUNT          597 a         1622 c         1591 g          675 t
ORIGIN      
        1 ggcagcgtct tccgcaaccc ggacggcgac tcggccgggc gcctgatcga cgagctcggc
       61 ctgaagggct atcgcatcgg cggcgccagc gtctcggaga agcacgctaa cttcatcgtc
      121 aacgaccagc gcggcagcgc ggccgacgtg cggcgcctcg ccgagcacgt tcgcagcgag
      181 gtcctcgccc gtcacgggac cgagctcgcg ttcgaggtcg agttcgcggg cgactggtcc
      241 ggctggccgc ccgaggaggc tgcctgatgt ccgtgccgcc cctcgcgcac gacccggccg
      301 cgacgcccgt cgccgtggtg ctgggcggtc catcggcgga gcacgacgtg tcgatcgtgt
      361 ccgggacggc gatcctggaa gcactgggcg gctccggtta ccccgcggag gcgtggctgg
      421 tcgaccttgg cggcggctgg tggcggctgc ccgatgggca ccggcgagat ggcaggccac
      481 aggcgctcta cgacgatccc gccgccgtgg ggggcacggg tccgcttcac gccgcggccg
      541 ccttggatgg gctggctgcg caggcccgcc cgccggtcgt gttcctcgcg ctgcacggtc
      601 cgttcggtga ggacggtacg gttcaggccc tgtgcgaggc cgccggcctc gcgtacacgg
      661 ggtcgggcgt caccgcgtcc gccatcggca tggacaaggc cgtgttcaag cggctgatcc
      721 gcgggctcgg gttgcccgtg gtcgattggc gcgaggtgcg cgcggcccgc tggtcgcgcg
      781 acccgggcag cgtcctcgcg gagctcgagg cgttcgccgc agggacgggc gacgcgcgcc
      841 tgatggtcaa gcccgcgcgc ctcggcagct ccgtcgggat gaccctggcc catacggtgg
      901 gggagcgagc cggggccctc gagaccgcgt tccgctacga cgatctggtg ctggtcgagc
      961 gctacctcgc cggcgcgcgc gacctggagg tcgcggtgat gggcaacgag cccgatgcgc
     1021 tcgagctcta cggcccaggc gagatcctcg ccggccacga gttctacgac tatgccgcca
     1081 agtacacgcc cgggctgtcc gagtcgacga cgagcgccga ggtgacgccc gccgagcgag
     1141 cggcgaccct caagctcgcc cgcgatgcct accgggcgac cggctgcgag ggcttcgcgc
     1201 gcgtggactt cctgctcgcc ggaccggagc tcgtcgtgtc cgagatcaac acgatcccgg
     1261 ggttcacgcc gatcagcctg ttcccgaccc tgcccgccgc cggcggctac gacttcgccg
     1321 ccgtctgcag ccgggtcgtg gacctcgcgc tcgaacgtgc cgcgggccgc gtccggcacc
     1381 ggctcaccgc cgccgacctg ccgcgctgac gatgcagcgt cgaggcgccg cgtcgcggca
     1441 gggagcgggg gtccgccgca cccgcaccgt ccgtcgacgc tcggccggcc tcaccccgac
     1501 gcgggccggc gccctgctcg cgctgctgct gtcgctcgcc gggctgtacg gagcgacgag
     1561 ctcgggtgcc ttctccatgc gtcgcctcga cgtctcgggc gtgacctgga cgtccgagga
     1621 cgcggtgctg gcggccctcg cgatccccga cggtcagaac ctgttcacgc tgagcaccgg
     1681 cgacctgatc gaccggctga cggtgctgcc cgcggttcgc gatgcctcga tcaccatcgc
     1741 cctgcccgac gaggtgcgag tggacgtggc cgagcgggtc gccctgctcg cctgggaggt
     1801 cgacggccag cgctacctgg tcgacgagga cgggctgctg ttcgcgcgcg tcgacgagac
     1861 gacgcccgcg gcagccgaca ggctgccgtt cgtcgatgac caccgtgtcg ccgccggggc
     1921 gctgaccgtg ggtgcgacgc tcgacccggt gatcctcgac gccgcccgac ggctcggctc
     1981 cctgcgtccc gccgacctcg gcacgtcggc cgcaagcctc gccattcgcc tcgacgacga
     2041 gaacggcttc acgcttcgag ccgagcccgt cgggtggtcg gccgtgttcg gcttctacac
     2101 cccgaccctg cggaccaccg acctcgtgcc gggccaggtg cgactgctcc ggagcatgct
     2161 cgccggccgc gaggatcgca tcctcaaggt gatcctcgcc gacgaccgga gcggcacgat
     2221 gatcccgcgc gaggacgcat caccgtcggg cgagcccgcg ccgtgaccac ccggcggccg
     2281 actccgcgag cgcgggtgtc tggtagcgta tcggggacct tcccccggac ggaggctgcc
     2341 cgcccgtgct cctgcccctc gtgggacttc ttgtcggcgt cgtcgcgggc ctcatcctca
     2401 acgtcaatgt cagcttcgaa ttctcgcgtt actcggccgt ggccatcctg gccgcactcg
     2461 actcggtcct gggggccgtc cgggcggagc tcgacggcgt ctacgacaat cggatcttca
     2521 tcagcgggtt cgtggtcaac gcgatcgtcg ccgtcctgct ggtgttcatc ggcgacaagc
     2581 tcagcctcga cctctacctg gtggccctga tcaacttcgg attccgcatc ttcaacaacg
     2641 tcgcgctgat ccggcgccac ttcatctagc agctagcgag gaacgacagg caggtggaac
     2701 gggaagcggt cctggtcggc atcgacgtcg gcacgtcaaa ggtctgtgcc ctgatcggcg
     2761 aggtggcccg cgacgggcgc ctgacgatca tgggccacgg gaccgtcccg gcctcgggcc
     2821 tcaagaaagg cgtcgtggtc aacatcgacc agaccgtccg ctcgatcgcc gacgccgtcg
     2881 agcgcgccga gcgcctgtcc ggctggaaga tcgaccgcgc cttcgtcggc gtcggcggca
     2941 accatgtcga gagcctcaac tcgaccggcc aggtcgcggt cacggcccac caccgcgagg
     3001 tcacccgcga ggacatcagt cgcgcgatcg aggtcgcgcg tgccgtctcg atccccaaca
     3061 accgcgacgt gcttcacgtc gagcgccggg gcttcacggt cgacggccag gagggggtca
     3121 aggacccgct cgggatgagc gcgctccgcc tcgaggtcga ggcccacatc gtctcggcgt
     3181 cggccaccgc cgtccagaac ctgaccaagt gcgtgcagct ggccggcgtc aagatcgacg
     3241 agctcgtcgc ggatgccctc gcgtcgtcgg aggccgtgct gaccgagacc gagaaggagc
     3301 tgggcgtcgc cgtcgccgac atcggcgccg gcacgatcga cctctcgctg ttcgccgatg
     3361 gctcgccctt ccacacctcg gtgctgccgg tcggcggcaa caacgtgacc aacgacgtcg
     3421 cgatcggcgt gaagaccacg ctccaggtcg cggaggagct caaggtccgc cacgggacgt
     3481 gcgacctgcg cgggctcgag gacgaagaga tcgccgtctc gatggtgggc gacgacgccg
     3541 gccggaccgt cagccgcgag gagctgtgcc agatcatcga ggcccggatg cgcgagacgt
     3601 tcgagctcct gcgcggcgag atgacccgca gcggccatgg gatgctgccc gctgggatca
     3661 tcctcaccgg cggcgcggcg cagcttgcgg gtacggctga gctcggccgg gaggtgctcc
     3721 agatgccggt ccgggtcggg tcgccatcgg ggatcgccgg cctcgtcgac acgatccagg
     3781 acccgtccta ctcgacggcc gtcggcctgc tccgctgggg ggcgatccag gtggcgcagg
     3841 gcgagccgct gcgctacgaa tcggcgccgg gcggtggcgg cttcggccgg atccgggacg
     3901 cgatccgctc gatcttcccg tagcggtctc ccggggttcg tctcccgtca gccagtcaaa
     3961 gcacgactgt tcggcgcgaa tctggggcag tcgactgacc gggggcggcc ctctcgtacc
     4021 atcgttcgcc gttggcggct aactgccgac gccatcctcc gccctgaagc cctcgtccat
     4081 cgacgacaag tcgtcctttg actcgatggc gggagattcg tggctcggca tcgggcgagg
     4141 cgcgcgagaa tggtcgccga tccagggaca cggacgggag gcagcgtgca atcgatccag
     4201 ctcgaggttc ggacggggcg tgaccgcggc ttcaccgacc tgacgcccgc ctgctcccgc
     4261 ttcgcggccg aggcctcgca gggcggcgac ggcctgctct cgctgttcgt gccgcatgcg
     4321 acggccggcg tggtgatcct cgagctgggc gcggggtccg acgtcgatgc ggttgcgaca
     4381 ctcgaccggc tgctgccgcg ggacgaccgc tggacgcatc gccacggatc cccgggccac
     4441 ggggcggacc acgtcctgcc gctgctcgcc gcgccctcga tcacc
//
LOCUS       DBNHXO010000562         2112 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4959167, whole genome shotgun sequence.
ACCESSION   DBNHXO010000562 DBNHXO010000000
VERSION     DBNHXO010000562.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4959167"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="AC162A_11315"
     CDS             <1..382
                     /locus_tag="AC162A_11315"
                     /inference="COORDINATES: protein motif:HMM:NF052759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR5011476.1"
                     /translation="GATRVVIEPPRRQGRGPLVREVIVDGFRFEVEVEDERLAALRER
                     ASRGRTTGQGGGPLEVRAVIPGKVGAVSVAAGDVVEAGQQLLVVEAMKMQNELRAPRS
                     GTIDRVGVAEGVNIEVGDLLLVIG"
     gene            393..2096
                     /locus_tag="AC162A_11320"
     CDS             393..2096
                     /locus_tag="AC162A_11320"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR5011477.1"
                     /translation="MSDETPRGATNGDEDEADATLDRWRSTTRARAVASSPERRAEFA
                     TTSEIPVADVYTQGDVDELDPGRDLGLPGEYPFTRGVQATMYRSRFWTMRQYAGFATA
                     AETNERFRYLLSQGQTGLSVAFDLPTQMGYDSDAPEAEGEVGRVGVPISSLADMEVLF
                     AGIPLGEVSTSMTINSTAPILLALYVAAAERQGVARAAVSGTTQNDILKEYIARGTWI
                     YPPRASMRLVTDVFEFAARELPKWNTISISGYHMREAGATAAQELAFTLADGIAYVEA
                     AVARGLAVDDFAPRLSFFFAAWSELFEEVAKFRAARRMWARIMRDRFGASNIRSMMCR
                     FHTQTAGSSLTAQSIDNNVVRTTIQALAAVLGGTQSLHTNSRDEALALPTAEAARLAL
                     RTQQIIAHEAGVTETPDPLAGSWFVESLTDQLEAAANAYLEEIDTMGGTLAAIEAGFQ
                     QRQIQEAAYRVQREVERGDRIVVGVNKFVDDDEGVRPAIQRIDPEGERRPIEGVRRVR
                     AERDPAAWEAAMRRLGEEAKGEANLLPAIIEAVEAYATVGEIADRLRSSWGVHRELIT
                     V"
BASE COUNT          313 a          726 c          792 g          281 t
ORIGIN      
        1 cggcgcgacc cgcgtcgtca tcgagccgcc ccgtcggcag ggcagggggc cgctcgtccg
       61 ggaggtcatc gtcgacggct tccggttcga ggtggaggtg gaggacgagc gcctggccgc
      121 cctccgggag cgcgcgtcgc gcgggcggac gaccgggcag ggtggcgggc ccctcgaggt
      181 ccgggcggtg atcccgggca aggtcggggc ggtctcggtg gcggccggcg acgtcgtcga
      241 ggccgggcag cagctgctgg tggtcgaggc gatgaagatg cagaacgagc ttcgagcccc
      301 acgaagtggc accatcgacc gggtaggggt tgccgagggc gtcaacatcg aggtcggcga
      361 cctgctgctg gtcatcggct agggaggtcc gggtgagcga cgagactccg aggggagcga
      421 cgaacgggga cgaggacgag gcggacgcca ccctcgatcg ctggcggtcg acgacgcgcg
      481 cccgcgctgt cgccagctcg ccggaacgtc gggccgagtt cgcgaccacc tcggagatcc
      541 cggtcgcgga cgtctacacg cagggcgacg tcgacgagct ggatcccggg cgtgacctcg
      601 gcctccccgg ggagtacccg ttcacccggg gcgtgcaggc gaccatgtac cggtcccggt
      661 tctggaccat gcgccagtac gccggcttcg ccactgccgc cgagaccaac gagcgcttcc
      721 ggtacctgct ctcccagggt cagaccggcc tgtcggtcgc cttcgacctg ccgacccaga
      781 tgggctacga cagcgacgca cccgaggcgg agggcgaggt cggccgggtg ggggtcccga
      841 tctcgtccct cgcggacatg gaggtgctgt tcgcggggat ccccctgggc gaggtcagca
      901 cctcgatgac gatcaactcc accgccccga tcctgctcgc cctgtatgtg gccgccgccg
      961 agcggcaggg ggtcgcgagg gcggccgtct cgggcacgac ccagaacgac atcctcaagg
     1021 agtacatcgc ccgggggacg tggatctacc cgccccgcgc ctcgatgcgc ctcgtgaccg
     1081 acgtgttcga gttcgccgcc cgtgagctgc ccaagtggaa cacgatcagc atctcgggct
     1141 accacatgcg cgaggcgggg gccacggcgg cccaggagct ggccttcacg ctcgccgatg
     1201 ggatcgccta cgtggaggcg gcggtcgccc ggggcctcgc ggtcgacgac ttcgcccccc
     1261 ggctgtcgtt cttcttcgcc gcctggtccg agctgttcga ggaggtcgcc aagttccggg
     1321 ccgcacgccg gatgtgggcc cggatcatgc gggatcgctt cggggcatcg aacatccgtt
     1381 cgatgatgtg ccgcttccac acgcagacgg ccgggtcgtc gctgaccgcc cagtcgatcg
     1441 acaacaacgt cgtgcggacc acgatccagg ccctcgcggc cgtgctgggc ggcacccaga
     1501 gcctgcacac gaactcgcgc gacgaggcgc tggccctgcc gacggccgag gcggcccgcc
     1561 tcgccctgcg cacgcagcag atcatcgccc acgaggccgg ggtcaccgag acgccggacc
     1621 cgctcgcggg cagctggttc gtcgagtcgc tcaccgacca gctggaggcg gcggcgaacg
     1681 cctacctcga ggagatcgac accatgggcg gcacgctagc cgcgatcgag gcgggcttcc
     1741 agcagcgcca gatccaggag gccgcgtacc gggtccagcg ggaggtcgaa cgcggcgacc
     1801 ggatcgtggt cggcgtcaac aagttcgtcg acgacgacga gggcgtccgg ccggcgatcc
     1861 agcggatcga cccggagggg gagcggcgcc cgatcgaggg tgtccgccgc gttcgggcgg
     1921 agcgcgaccc ggccgcctgg gaggccgcaa tgcgccgcct cggtgaggag gcgaagggcg
     1981 aggccaacct gttgccggcg atcatcgagg ccgtcgaggc gtacgcaacg gtcggcgaga
     2041 tcgccgaccg gctccgctcg tcgtggggcg tccaccgcga gctcatcacg gtctgaggag
     2101 gagcgaccga tg
//
LOCUS       DBNHXO010000563         2891 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3369966, whole genome shotgun sequence.
ACCESSION   DBNHXO010000563 DBNHXO010000000
VERSION     DBNHXO010000563.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3369966"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="AC162A_11325"
     CDS             complement(<1..164)
                     /locus_tag="AC162A_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5011478.1"
                     /translation="MTPIRLLIVDDHPVVRDGLRGMFSGDPEFDVVGEAADGAEAIEL
                     AARLGPDVIL"
     gene            complement(161..1423)
                     /locus_tag="AC162A_11330"
     CDS             complement(161..1423)
                     /locus_tag="AC162A_11330"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017563210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5011479.1"
                     /translation="MSGSPSPGSTAAAPSLYGRWGDRLNDWCPYVTLAVSSVLTFAWT
                     DDPAGRPLTVALIALAALWVYVGYTRAPRPRSAQRARMLVYFIGLLAIASVLAVRDYL
                     FFIFLITGFFHAMMLRPWPVLIAGVFATSVLVNTVIGGLPTNPEWWAIWVVIVVVQTL
                     AIGGGGILGERMAEQNEERRQAVARLEVALEENAGLHAQLLTQAREAGVLDERGRMAR
                     EIHDTIAQGLTGIVTQLEAAEQARDRPDDWQRHVRNAIGLARDSLSEARRSVEGSRPE
                     YLETARLPEALAEVARQWSELNGIPVEVATTGDVQPLHAEVEVALLRTAQEALANVAR
                     HAKASRAWLTLSYMGDVVTLDVRDDGIGFEVPAASDERGSGFGLSGMRQRVNRVAGTL
                     AIESEPGAGTAISARVPAIVSPGMVGRA"
     gene            complement(1420..2145)
                     /locus_tag="AC162A_11335"
     CDS             complement(1420..2145)
                     /locus_tag="AC162A_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5011480.1"
                     /translation="MLHHMITAETRLFFREPGVWLLTILLPTIVLVAVGLVFGVEPDP
                     GLGGLRWIDIFAPSMVVMTLAILGVNLLPARLVKYREKGVLRRLSTTPASPRSVLIAQ
                     LVVNMGVAVVSLAVLLVVGNLMFGIPLPKDPIGFAVAFLLGMSALFALGLLVAAVAPS
                     AGIASALFVPLFALVMLLGGVYLPRTMLPELLIRIGDYTPPGVQALLDAWQGTSPQLA
                     QLAVMALITVLAGAAAAKRFHWE"
     gene            complement(2177..>2891)
                     /locus_tag="AC162A_11340"
     CDS             complement(2177..>2891)
                     /locus_tag="AC162A_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5011481.1"
                     /translation="DPQRDRDELRRVVGVQLQESELPEQMRVREAVELYASFYAGPRD
                     GEQLLADLGLADKRKSQFGKLSGGEKQRLSVALALIGNPKVAILDELTTGLDPQARRD
                     TWQLIEDVRAGGVTVILVTHFMEEAERLADRIALIDAGRVVALDTPSGIVSRVDAEQT
                     LRFRPSVAMDDGLLTGLPEVRRVSRHGPTVEVAGTGNLLAAVTAVLARNHIVANELRI
                     EQAGLDDAFVALTGRKLAS"
BASE COUNT          460 a         1019 c         1040 g          372 t
ORIGIN      
        1 atcaggatca cgtccggacc gagacgggcc gcgagctcga tcgcttcggc gccatcggcc
       61 gcctcgccga cgacgtcgaa ctcggggtcg ccggaaaaca tgccccggag gccgtcgcga
      121 accaccgggt ggtcgtcgac gatgagcagc cggatcggcg tcacgcgcga ccgaccatgc
      181 ccggcgagac gatcgccggg acacgtgccg agatggccgt cccggcgccg ggctcggact
      241 cgatcgcgag cgtcccggcg acccggttga cccgctggcg catccccgac agcccgaagc
      301 cggaccctcg ctcgtcgctc gcggccggga cctcgaagcc gatcccgtca tcgcggacat
      361 ccagcgtcac cacgtcgccc atgtaggaca gggtcagcca ggcgcgcgac gccttggcgt
      421 gcctggcgac gttggccagg gcctcctgtg cggtccgtag cagggccacc tccacctcgg
      481 cgtggagagg ctggacgtcg ccggtggtcg ccacttcgac ggggatcccg ttcagctcgg
      541 accactgccg cgcgacctcc gccagcgcct cgggcagccg cgcggtctcg aggtattcgg
      601 ggcgtgagcc ctcgaccgag cgccgcgcct ccgacaggct gtcgcgcgcg aggccgatcg
      661 cgttccgcac gtgccgctgc caatcgtcgg gccggtcgcg cgcctgctcg gcggcctcga
      721 gctgggtgac gatcccggtg aggccctggg cgatcgtgtc gtggatctcg cgcgccatgc
      781 gcccgcgctc gtcgagcaca cccgcctcgc gcgcctgggt cagcagctgg gcgtggaggc
      841 cggcgttctc ctcgagcgcc acctcgagcc gggccaccgc ctggcgccgc tcctcgttct
      901 gctcggccat gcgctcgccg aggatgccgc cgcccccgat cgcgagcgtc tggacgacga
      961 cgatcaccac ccagatcgcc caccactcag ggttcgtcgg gagcccgccg atcacggtgt
     1021 tgaccaggac cgacgtggcg aacacgccgg cgatcaggac gggccagggc cgcagcatca
     1081 tcgcgtggaa gaagccggtg atcaggaaga tgaagaacag gtagtcgcgg acggccagca
     1141 ccgatgcgat cgccagcagg ccgatgaagt acacgagcat ccgcgcccgc tgggccgagc
     1201 gcggacgtgg tgcccgcgtg tagccgacgt acacccacaa ggcggcgagt gcgatcaggg
     1261 ccaccgtcag tggacgccct gccgggtcgt cggtccacgc gaaggtgaga accgaggaca
     1321 cggccagggt gacgtacgga caccagtcgt tcagccggtc gccccatcgc ccgtagagcg
     1381 atggggcagc ggcggtcgat cccggcgagg gtgacccgct cattcccagt ggaatcgctt
     1441 cgcagcggcg gcgcccgcca ggacggtgat cagggccatc accgccagct gggcgagctg
     1501 cggcgacgtc ccctgccagg catcgaggag ggcctggacg ccgggcggcg tgtagtcacc
     1561 gattcggatc agcagctcgg gcagcatcgt gcgtggcagg tataccccac cgaggagcat
     1621 gacgagcgcg aacagcggca cgaacagcgc gctggcgatg ccggccgagg gagccacggc
     1681 ggcgaccagg aggccgaggg cgaacagcgc tgacatgccg agcaggaacg cgacggcgaa
     1741 cccgatcggg tccttcggca gcgggatgcc gaacatgagg ttgccgacga cgaggagcac
     1801 cgcgaggctc acgacggcca cgcccatgtt gaccacgagc tgggcgatga gcaccgaccg
     1861 cgggctggcg ggggtcgtcg acaagcggcg aaggacaccc ttctcgcggt acttcaccag
     1921 gcgcgccggc aggagattca cgcccaggat cgccagcgtc atgacgacca tcgacggcgc
     1981 gaagatgtcg atccagcgca gcccgccgag ccctggatcc ggctcgacgc cgaagacgag
     2041 cccgacggcg accaggacga tggtcggcag caggatcgtg aggagccaca ccccgggctc
     2101 gcgaaagaac agccgggtct cggcggtgat catgtgatgc agcatggcag tctcctgggg
     2161 aagtcagtgg gaggggtcag gacgcgagct tgcggccggt gagcgcgacg aacgcgtcgt
     2221 cgagaccggc ctgctcgatc cgcagctcgt tggccacgat gtggttgcgc gcgaggacgg
     2281 ccgtgacggc cgccaggagg ttgccggtgc ccgcgacctc gacggtcggg ccgtggcggc
     2341 tcaccctgcg aacctcgggc agccccgtca gcagcccgtc gtccatcgcg acggacggcc
     2401 ggaaccggag cgtctgctcg gcgtcgacgc gcgacacgat gcccgagggc gtgtcgaggg
     2461 ccacgacgcg gcccgcgtcg atcagcgcga tgcggtcggc gaggcgctcg gcctcctcca
     2521 tgaagtgggt cacgaggatg acggtcacgc ccccggcccg gacgtcctcg atcagctgcc
     2581 aggtgtcacg cctggcctgc gggtcgagcc ccgtcgtcag ctcgtcgagg atcgcgacct
     2641 tcgggttgcc gatgagcgcg agggcgaccg agagccgctg cttctcgccg cccgacagct
     2701 tgccgaactg ggacttgcgc ttgtccgcga gcccgaggtc ggccagcagc tgctcgccgt
     2761 cccgcggccc cgcatagaac gaggcgtaga gctcgacggc ttcccggacg cgcatctgct
     2821 ccggcagctc gctctcctgg agctgcacgc ccacgacgcg acgaagctcg tcgcggtcgc
     2881 gctgcgggtc g
//
LOCUS       DBNHXO010000564         2420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_792432, whole genome shotgun sequence.
ACCESSION   DBNHXO010000564 DBNHXO010000000
VERSION     DBNHXO010000564.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_792432"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="AC162A_11345"
     CDS             <1..873
                     /locus_tag="AC162A_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5011482.1"
                     /translation="STALRALRDWARGEVAYPAIRRHLEAGNETETIAEMVPELKKLA
                     KLSRDHLTSANLRLVVSIARKYINQAGMGLLDLVQEGNAGLIRAVDKFEYERGFKFST
                     YATWWIRQAIQRGLADQSRTIRIPVHMVETMGRVARVVRELSTQLGREPTNTEIAAAL
                     PDETPAMTPQRVEEIRKLGREPVSLQSPVGDEGDAELGELIPDEDAVSPLDAVADRMR
                     REQIEHVLNSLDGREQRVLRLRFGLDDGHARTLEEVGREFGLTRERIRQIEVQALRKL
                     RHPSRSRKLREFAA"
     gene            complement(929..>2420)
                     /locus_tag="AC162A_11350"
     CDS             complement(929..>2420)
                     /locus_tag="AC162A_11350"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR5011483.1"
                     /translation="AETAVAILRDEPPTAERARVLAGYGQMLMLLDHWSESRAVCEEA
                     IEIALRVGAREAEGHARNSLGLDLAAQGRCEEALESLETAIVIAEEVGNADDVGRAHV
                     NLAEALNVCGLVHEAADAVVRGIAAAEAYGISSSYGSFIRHNGILINYDLGRWETAVR
                     LAGESATSLSESGASNPDRYLASHWAPLLVATGQFEQARAQLERLGELLEGAPLEAQF
                     SGNYVIGRAELALWEGRTLDALAEVDRGLAWLGDEGWRWFVTRLLRLGARAAADAVGL
                     ARARRDPAGEAEALARADAYRERRVRHRGACEAAEHGPQLAVSLAEFAMAEAEDTRPD
                     GGGDVASWLDVRDQWLALERPYVAAYASWRLGEARLEAGDRDGAATDLRDAHATATGL
                     GALPLTTAIEALAARARIDLRPASEALLPPTQADPFGLTRREREVLALVATGRTNRQI
                     ADELFISENTAGVHVSNILGKLGVASRTEAAAVAVRLDMGAAPEAG"
BASE COUNT          335 a          914 c          850 g          321 t
ORIGIN      
        1 agcacggccc ttcgcgccct gcgcgactgg gctcgcggcg aggtcgccta cccggcgatc
       61 cggcgccacc tcgaggcggg caacgagacc gagaccatcg ccgagatggt gccggagctc
      121 aagaagctgg ccaagctgtc gcgggaccac ctgacgtcgg ccaacctgcg cctcgtcgtg
      181 tcgattgccc gcaagtacat caaccaggcc ggcatgggcc tcctcgacct cgtccaggag
      241 ggcaacgcgg gcctgattcg cgccgtcgac aagttcgagt acgagcgcgg cttcaagttc
      301 agcacctatg ccacctggtg gatccgccag gcgatccagc gcggcctcgc ggaccagagc
      361 cggacgatcc ggatcccggt ccacatggtc gagacgatgg gtcgcgtggc gcgcgtcgtg
      421 cgcgagctgt cgacgcagct cgggcgtgag cccaccaaca ccgagatcgc ggcggccctc
      481 cccgatgaga cgccggcgat gacgccgcag cgcgtcgagg agatccgcaa gctcgggcgc
      541 gagcccgtct cgctccagtc gccggtcggc gacgagggcg acgcggagct cggcgagctg
      601 atccccgacg aggacgcggt gtcgcccctc gacgcggtcg cggaccggat gcgccgggag
      661 cagatcgagc acgtgctaaa cagcctcgac ggccgcgagc agcgcgtcct gcgcctgcgg
      721 ttcggcctcg acgacgggca tgcccgcacg ctcgaggaag tcggacgcga gttcggcctg
      781 acccgcgagc ggatccgcca gatcgaggtc caggcgctgc gcaagctgcg ccacccgtcg
      841 cggagccgca agctgcgcga gttcgccgcc tagggcgatc caccttcgcc acacctggag
      901 ccgcggtcct cgaccgcggc ttcttccgtt aaccggcctc gggcgcggcc cccatgtcga
      961 ggcgcacggc gaccgcggcg gcctcggtgc gggaggcgac gccgagcttg cccaggatgt
     1021 tcgagacgtg gacgcccgcc gtgttctcgc tgatgaacag ctcgtcggcg atctggcggt
     1081 tggtccggcc cgtggcgacc aatgccagca cctcgcgctc ccgccgggtc agcccgaacg
     1141 ggtcggcctg cgttggcggg agcaacgcct ccgacgcggg tcgcaggtcg atccgggcgc
     1201 gggcggcaag cgcctcgatg gcggtcgtga gcggaagggc gccgagaccc gtcgccgtcg
     1261 cgtgggcgtc ccgcaggtcc gtcgccgcgc cgtcacgatc gccggcctcg aggcgtgcct
     1321 cgccgaggcg ccaggacgcg taggcagcca cgtatggccg ctccagcgcg agccactggt
     1381 ctcggacgtc gagccacgag gcgacatcgc cgccaccgtc cggtcgcgtg tcttcggctt
     1441 ccgccatcgc gaactccgca agggagacgg cgagctgtgg cccgtgctcc gccgcctcgc
     1501 atgccccgcg gtgccggacc cgccgctcgc gatacgcgtc cgcccgtgca agcgcctccg
     1561 cctcgcccgc cgggtcgcga cgcgcacgcg cgagtccaac cgcgtcggcg gccgcccggg
     1621 ctcccaggcg caggaggcgg gtcacgaacc agcgccagcc ctcgtcgccg agccacgcca
     1681 gtccacggtc gacctcggcc agcgcgtcga gcgtccgccc ctcccacagg gcaagctcgg
     1741 cccggccgat gacgtagttg ccgctgaact gggcctcgag cggcgcgccc tcgagcagct
     1801 cgccgaggcg ctccagctgc gccctcgcct gctcgaactg gccagtcgcg acgagcagcg
     1861 gtgcccagtg gctggccagg taacggtcgg ggttggaggc tccgctctcc gagagcgacg
     1921 tcgcgctctc gcccgcgagc cgcaccgcgg tctcccagcg cccgaggtcg tagttgatca
     1981 ggatcccgtt gtggcggatg aacgagccgt aggagctcga gattccgtag gcctcggccg
     2041 cggcaatgcc ccggacgacc gcgtcggcgg cctcgtgcac gagcccgcac acgttgaggg
     2101 cctcggccag gttcacgtgc gcgcgtccga cgtcatccgc gttgccgacc tcctcggcga
     2161 tgacgatggc cgtctcgagc gactccaggg cctcctcgca ccggccttgg gcggccaggt
     2221 cgaggccgag actgttccgc gcgtgaccct cggcctcgcg ggctcccacg cgcagcgcga
     2281 tctcgatcgc ctcctcgcac acggcgcggg actcgctcca gtgatcgagc agcatcagca
     2341 tctggccgta ccccgccagc acgcgggccc gctcggccgt gggcggctcg tcgcgcagga
     2401 tcgcgacggc cgtctcggcc
//
LOCUS       DBNHXO010000565         5087 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3569063, whole genome shotgun sequence.
ACCESSION   DBNHXO010000565 DBNHXO010000000
VERSION     DBNHXO010000565.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3569063"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="AC162A_11355"
     CDS             <1..1208
                     /locus_tag="AC162A_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR5011484.1"
                     /translation="KTRLARLLVGLLDEWLPVVRGGELNDDPFAPISAGARAIVEAQG
                     DATPIDWLPRDRRYAEKLATPDITIADLIGEVDPIRVAEGRYLSDELTLHYGLIPRAN
                     RGIFTLNELPDLAERIQVGLLNVLEERDVQIRGFTIRLPLDLLVVASANPEDYTSRGR
                     IITPLKDRLGSQIRTHYPRSLDHEIAIVRQEKQAFPERAEIPPVATPGFMEELVAELT
                     HLARRSPEISQRSGVSVRVSVANMEVLEAAAIKRAVRLREPMASPRISDLGAVLAATT
                     GKVEVETVGEDTPEERIVERLVTKAVFASFNRRASLDRLAPVIEAFEDGLIVETGERV
                     PSREYVRWMGEVPGLRDAVVRLGTFDVTDGAEEPALVASAVEFLLEGLHLQRRLNKDR
                     GAAGVTYRR"
     gene            1227..3344
                     /locus_tag="AC162A_11360"
     CDS             1227..3344
                     /locus_tag="AC162A_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR5011485.1"
                     /translation="MAFLTPDPRHLGRPDAGLGGRDRPLPPAAKYRRWDGSQDLPDLT
                     ADEIVDALADDVLEHGDVGEALRRLMERGLRGSDPSRGDLRGLRDMLDRLRDRREEIL
                     RQGALSDPLADVRRELDEIVEAEREGVQQRLDAGAEADAEGNRADPELERMLREIAAK
                     RLDTLDALPPDVGARIRGLGDYDFMDPSARERFEALVDRLRGSVLDRLSEGLADAVKG
                     MRPEDLAAQRSMVQDLNRLLARRLSGDEPSRADVDEFLAQHGSFFPGAATLDDVVDQL
                     AERMAAMQSLMRSLSPQQRAELQDTIDALLRDDRLRWDLAQLAANLDQLLPDGLGDRV
                     RFGGDEPMGLEAALDQLGRLQALDRLEADLEGIGGEGDLADIDRADVRDLLGPDAVRD
                     LDALEDLAQRLEQAGYLDREGGRLELTPRGSRRIGQKVLDDLFARLRKDAFGGHRLDR
                     TGRGGDREETAKPFEFGDPFHLDLRATMTNALMREENAPARRPAGSAVRLRPDDFEVF
                     RTERTTSASTVLLVDMSRSMLLRGCYLAAKRVAIALDTLIRTQFPRDHLSIVGFAYYA
                     RELRPEALAELGWNTYEYGTNLQHGLLLARRILARHHAANREIVVITDGEPTAHFENG
                     QVEFSYPPTRRTIQETLREVVRCTKDGITINTFMLERSPALTDFVGLVTELNRGRAFY
                     AEPERLGEYVLVDFVQRRTRRVS"
     gene            3390..3872
                     /locus_tag="AC162A_11365"
     CDS             3390..3872
                     /locus_tag="AC162A_11365"
                     /inference="COORDINATES: protein motif:HMM:NF021538.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HIR5011486.1"
                     /translation="MDQLTIFRLFLFLHVLGAIVAFGPGFAAAIAGAMVAKEPQHANF
                     FARTQVAIGMKLVTPAAISMLITGVIMIGVMGYSTIIGGRQWLTVAVILYVIAIGYSI
                     AVQAPAGRRLVALTETPPAPGSPPPPELPATAKKVRTGGIFLSTLVVIIVFLMVVKPF
                     "
     gene            3943..>5087
                     /locus_tag="AC162A_11370"
     CDS             3943..>5087
                     /locus_tag="AC162A_11370"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR5011487.1"
                     /translation="MPNVAESPADLIASLTGAAHLADGRLVIDEEAGFRSRVAADLAW
                     TAAFTSDDPTAEAARWLVWEASQALGARSASIQDLYLARGRGEVSGFTVPAINIRAQT
                     FDMARTFYETAARADVGAVIFELARSEQTYTFQRPMDYATFVLAGAIAAGWRHPVFIQ
                     GDHYQFNAKKYAADPEAMTEEIRRACRLAIDAGYRNIDIDSSTLVDLSKADRDEEQRE
                     NYVRAAELTALIRGLETDGVTVSVGGEIGEVGKQNSTADELEAYLDGYDRELARRSPG
                     APGLSKVSVQTGTSHGGVPLPGGGVAEVKLDFEVLRHLGEIARARGLAGAVQHGASTL
                     PEELFHHFPAVETAEIHLATGFQNALYEHPAFPSSLMDEIYAWCGVNA"
BASE COUNT          746 a         1879 c         1775 g          687 t
ORIGIN      
        1 cgaagacgag gctcgcacgc ctgctcgtcg gcctcctcga cgagtggctg cccgtcgtcc
       61 gcggcggcga gctcaacgac gacccgttcg cgccgatcag cgccggcgcg cgggcgatcg
      121 tcgaggcgca gggcgacgcg acccccatcg actggttgcc gcgcgaccgc cggtatgccg
      181 agaagctggc cacccccgac atcacgatcg ccgacctgat cggcgaggtc gatccgatcc
      241 gcgtcgccga aggccgctac ctgtccgacg agctgaccct gcactacggg ctgatcccgc
      301 gcgccaaccg gggcatcttc accctcaacg agctgccgga cctcgccgag cggatccagg
      361 tcggcctgct caacgtcctc gaggagcgtg acgtccagat ccgcgggttc acgatccgcc
      421 tgccgctcga cctgctcgtc gtcgcgtccg ccaacccgga ggactacacc agccgcggcc
      481 ggatcatcac ccccctcaag gatcgcctgg ggtcgcagat ccgaacccat tacccgcgct
      541 cgctcgacca cgagatcgcg atcgtccgcc aggagaagca ggccttcccc gagcgcgccg
      601 agatcccgcc cgtcgcaacc ccgggcttca tggaggagct ggtcgccgag ctgacccacc
      661 tcgcccgtcg gtcgcccgag atcagccagc gcagcggcgt gtccgtgcgc gtgtcggtcg
      721 cgaacatgga ggtcctggag gccgcggcga tcaagcgtgc ggtccggctg cgcgagccga
      781 tggcctcgcc ccggatctcg gacctcggcg ccgtgctcgc ggcgacgacg ggcaaggtcg
      841 aggtcgagac ggtgggcgag gacacgcccg aggagcgcat cgtcgagcgc ctggtcacga
      901 aggccgtgtt cgcgagcttc aaccggcggg cctcgctgga ccgcctcgcg ccggtgatcg
      961 aggcgttcga ggatgggctg atcgtcgaga ccggcgagcg cgtgccatcc cgcgagtacg
     1021 tccgatggat gggcgaggtg ccgggcttgc gggacgcggt cgtgcggctc ggcacgttcg
     1081 acgtcaccga cggggccgag gagcccgccc tcgtggcatc cgcggtcgag ttcctgctcg
     1141 agggactcca cctccagcgc cgcctcaaca aggaccgcgg cgccgccggc gtcacctacc
     1201 ggcgctgagg gcagacgccc gagccaatgg ccttcctgac gcccgatccc cgccacctcg
     1261 gacggcccga cgccgggctg ggcggccgtg accggccgct gccgccggcc gccaagtacc
     1321 ggcgctggga cggcagccag gacctgcccg atctcaccgc cgacgagatc gtcgatgccc
     1381 tcgcggacga cgtgctcgag catggggacg tcggcgaggc gctgcgtcgc ctcatggagc
     1441 ggggcctgcg cggcagcgat ccgtcgcgcg gcgacctccg aggcctgcgg gacatgctcg
     1501 acaggctgcg ggatcgccgc gaggagatcc tgcgccaggg cgcgctgtcg gacccgctgg
     1561 cggacgtgcg gcgcgagctc gacgagatcg tcgaggcgga acgcgagggc gtccagcaac
     1621 gcctggacgc cggcgccgag gcggacgccg aggggaaccg cgccgacccc gagctcgagc
     1681 ggatgctccg ggagatcgcg gccaagcgcc tcgacacgct cgacgcgctg ccgcccgacg
     1741 tcggggcgcg gatccggggc ctcggggact acgacttcat ggacccctcg gcgcgcgagc
     1801 gcttcgaggc gctggtcgat cgcctgcggg gcagcgtgct cgaccggctg agcgagggcc
     1861 tggccgacgc ggtgaagggc atgcggcccg aggacctcgc cgcgcagcgc tcgatggtcc
     1921 aggacctcaa ccggctgctg gccaggcggc tctcgggcga cgagcccagc cgcgcggacg
     1981 tcgacgagtt cctcgcccag cacggcagct tcttcccggg cgccgcaacg ctcgacgacg
     2041 tcgtggacca gctcgccgag cggatggccg cgatgcagtc actgatgcgg tcgctctcgc
     2101 cgcagcagcg cgccgagctc caggacacga tcgacgccct gctgcgcgac gaccggctgc
     2161 gctgggacct cgcccagctg gcggccaacc tcgaccagct gttgcccgac ggcctgggcg
     2221 atcgggtccg gttcggtggc gacgagccca tgggcctcga ggcggcgctg gatcagctcg
     2281 ggcggctgca ggcgctcgac cgtctcgagg cggatctcga ggggatcggg ggcgagggcg
     2341 atctcgccga catcgatcgt gccgatgtcc gtgacctcct cgggccggac gcggtccgcg
     2401 acctcgacgc cctcgaggac ctcgcgcaac gcctggagca ggcgggctac ctcgaccgcg
     2461 agggcggccg cttggagctc acgccgcgcg gcagccggcg gatcgggcag aaggtgctcg
     2521 acgacctgtt cgcgcggctg cgcaaggacg ccttcggagg gcaccggctg gaccggacgg
     2581 gcaggggagg cgaccgcgag gagaccgcca agccgttcga gttcggcgat ccgttccacc
     2641 tcgacctgcg cgcgacgatg accaacgcgc tgatgcgcga ggagaacgca cccgcccggc
     2701 gacccgcggg ctcggcggtc cgcctccgcc ccgacgactt cgaggtgttc cgcaccgagc
     2761 gcacgacctc ggcctcgacc gtcctcctcg tcgacatgag ccgctcgatg ttgctgcggg
     2821 gctgctacct cgccgccaag cgcgtcgcga tcgccctcga cacccttatc cggacccagt
     2881 tcccgcgcga ccacctctcg atcgtcggct tcgcctacta cgcccgcgag ctgcgcccgg
     2941 aggcgctcgc cgagctcggc tggaacacgt atgagtacgg caccaacctc cagcacggct
     3001 tgctgctcgc gcgccggatc ctcgcccgcc accacgccgc gaaccgcgag atcgtggtca
     3061 tcaccgacgg tgagccgacc gcgcacttcg agaacggcca ggtcgagttc agctatccgc
     3121 cgacccggcg cacgatccag gagacgttgc gcgaggtcgt ccgctgcacc aaggacggga
     3181 tcaccatcaa caccttcatg ctcgagcggt cacccgccct caccgacttc gtcggcctgg
     3241 tgaccgagct caaccgcggc cgcgcgttct acgcggagcc ggagcgcctc ggcgagtacg
     3301 tgctggtcga tttcgtccag cggcgcacac gccgggtgtc gtagagtccg cggcgcgacg
     3361 ggccgtcagg accgcgacag gaggcgtcga tggaccagct cacgatcttc cggctgttcc
     3421 tgttcctgca cgtcctgggc gcgatcgtcg ccttcgggcc ggggttcgcg gcagccatcg
     3481 ctggggcgat ggtggcgaaa gagccgcagc atgcgaactt cttcgcccgg acccaggtcg
     3541 ccatcgggat gaagctcgtc acccccgcgg ccatcagcat gctgatcacc ggcgtgatca
     3601 tgatcggtgt catgggctac agcacgatca tcggcggccg gcagtggctg accgtggcgg
     3661 tgatcctgta cgtgatcgcg atcgggtact cgatcgccgt ccaggcgccg gcggggcgcc
     3721 ggctcgtggc cctcaccgag accccgcccg ccccgggctc cccgccgccg cccgagctgc
     3781 cagcgaccgc gaagaaggtc cggaccggcg ggatcttcct ctcgaccctg gtcgtgatca
     3841 tcgtgttcct gatggtcgtc aagcccttct gatctggtcg gcggcccggg gccgcccatc
     3901 gcggctacca tctgggccga gacccacacg aggacgagcc gcatgccgaa cgtcgccgag
     3961 tcgcccgccg acctcatcgc cagcctgacc ggcgccgccc acctggccga tggccggctc
     4021 gtgatcgacg aggaggccgg cttccggagc cgggtggccg ccgatctcgc gtggacggcc
     4081 gcgttcacga gcgacgaccc cacggccgaa gcggcccgct ggctcgtgtg ggaggccagc
     4141 caggcgctgg gcgcccgatc cgcgagcatc caggacctct accttgcccg cggccgtggc
     4201 gaggtctccg ggttcaccgt gccggcgatc aacatccgcg cccagacgtt cgacatggcc
     4261 cgcacgttct acgagacggc cgcgcgcgcc gacgtgggcg ccgtgatctt cgagctcgcc
     4321 cgcagcgagc agacctatac gttccagcgg ccgatggact acgcgacgtt cgtgctggcg
     4381 ggcgcgatcg ccgccggctg gcggcacccg gtgttcatcc agggcgatca ctaccagttc
     4441 aacgccaaga agtacgccgc ggaccccgag gcgatgaccg aggagatccg ccgggcgtgc
     4501 cggctcgcga tcgacgccgg ctatcgcaac atcgacatcg acagctccac gctcgtcgac
     4561 ctgtccaagg ccgaccggga cgaggagcag cgcgagaact acgtccgcgc ggccgagctg
     4621 acggcgctga tccggggcct cgagaccgac ggcgtcaccg tgagcgtcgg gggcgagatc
     4681 ggcgaggtcg gcaagcagaa ctcgacggcc gacgagctcg aggcgtacct cgacggatac
     4741 gaccgcgagc tggcgcggcg ttcgccgggc gcgccgggcc tgtccaaggt gagcgtccag
     4801 accggcacct cgcacggagg cgtgccactc cccggcggcg gcgtcgccga ggtcaagctc
     4861 gatttcgagg tcctgcgcca cctgggcgag atcgcccggg ctcgcgggct cgccggcgcc
     4921 gtccagcacg gcgcgtccac gctccccgag gagctgttcc accacttccc ggcggtcgag
     4981 acggccgaga tccacctggc cacgggcttc cagaacgccc tctatgagca cccggcgttc
     5041 ccgtcgtcgc tgatggacga gatctacgcc tggtgcggcg tcaacgc
//
LOCUS       DBNHXO010000566         1777 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4164624, whole genome shotgun sequence.
ACCESSION   DBNHXO010000566 DBNHXO010000000
VERSION     DBNHXO010000566.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4164624"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="AC162A_11375"
     CDS             <1..627
                     /locus_tag="AC162A_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR5011488.1"
                     /translation="VTGFARIMGYPVGIVANNGILFSESSLKGAHFIELCTKRRIPLV
                     FLQNITGFMVGREYENRGLARDGAKMVMAVANARVPKFTVIVGGSFGAGNYGMCGRAY
                     SPRQLWMWPNARISVMGGQPAASVLLQVRLDALAREGRDMTPAEREAFTAPTLEAYER
                     EGSPYFSTARLWDDGIIDPADTRMVLGLGISAALNAPIPDTPFGVFRM"
     gene            640..>1777
                     /locus_tag="AC162A_11380"
     CDS             640..>1777
                     /locus_tag="AC162A_11380"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009024851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR5011489.1"
                     /translation="MFSSVLVANRGEIALRVMRTCARLGIRTVAVYSDADWAAPHVRA
                     ADDAVRLGPGPAGESYLRAEAILDAARATGAEAIHPGYGFLSENAGFADACLAAGLAW
                     IGPPPGAMRALGDKARAKALAEANGVPVLPGYHGDDTADEALTEAAATIGYPLLVKAS
                     AGGGGRGMRVVREPGELGEALAAARREAAASFGDDRLLLERFVERPRHVVVQILGDAH
                     GALVHLGERECSIQRRHQKLIEESPSPAVSPALRSSMGEASLRLARAAGYEGAGTVEF
                     LLDDGGAFSFLEVNARLQVEHPVTEAVTGLDLVEQQLRVAAGEPLAFAQADVRLDGHA
                     MELRIVAEDAAAGFLPSTGVVTAWDMPERVRVDTGVALGSTVSPF"
BASE COUNT          224 a          610 c          700 g          243 t
ORIGIN      
        1 gtcaccggct tcgcgcggat catgggctac ccggtgggca tcgtcgccaa caacgggatc
       61 ctgttcagcg agagctcgct caagggcgcc cacttcatcg agctgtgcac gaagcgccgc
      121 atcccgctcg tgttcctcca gaacatcacc ggcttcatgg tcggccgcga atacgagaac
      181 cgggggctgg cccgggacgg cgcgaagatg gtgatggcgg tcgcgaacgc gcgggtgccg
      241 aagttcacgg tcatcgtcgg cgggtcgttc ggggccggca actacggcat gtgcgggcgc
      301 gcctactcgc cacgccagct gtggatgtgg ccgaacgcgc ggatcagcgt catgggcggc
      361 cagccggccg catcggtcct gctgcaggtc cgcctcgacg ccctggcccg cgagggccgg
      421 gacatgaccc cggccgaacg cgaggcgttc acggcgccga cgctggaggc ctacgagcgc
      481 gagggctcgc cctacttctc gacggcccgc ctgtgggacg acggcatcat cgacccagcc
      541 gacacccgga tggtgctggg gctgggcatc tccgcggcgc tcaacgcgcc gatcccggac
      601 acgccgttcg gcgtgttccg gatgtagcgt ccgcgcgggg tgttctcgtc ggtcctcgtc
      661 gcgaaccggg gcgagatcgc gctgcgcgtg atgcggacct gcgcgcggct ggggatccgc
      721 acggtcgccg tgtactccga cgccgactgg gcggcgccgc acgtccgtgc cgccgacgat
      781 gccgttcgcc tgggccccgg gccggccggc gagagctacc tgcgggccga ggcgatcctc
      841 gacgcggccc gcgcgacggg cgcggaggcg atccacccgg gctacgggtt cctgagcgag
      901 aacgccgggt tcgccgacgc gtgccttgcc gcggggctgg cctggatcgg tccgccgccg
      961 ggcgcgatgc gggcgctcgg ggacaaggcg cgggcgaagg cgctggccga ggcgaacggg
     1021 gtgccggtcc tgcccggcta ccacggcgac gacacggctg acgaggccct gaccgaggcg
     1081 gccgcgacga tcgggtaccc gctgctggtg aaggcgagcg cgggcggcgg cgggcggggg
     1141 atgcgcgtcg tccgcgagcc cggcgagctc ggtgaggcgc tggcggcggc acggcgggag
     1201 gccgcggcgt cgttcggcga cgaccgcctg ctcctggagc ggttcgtcga gcgcccgcgg
     1261 cacgtcgtgg tgcagatcct cggcgacgcc cacggcgcgc tcgtccatct cggcgagcgc
     1321 gagtgcagca tccagcgacg gcaccaaaag ctgatcgagg aatcgccgtc gcccgccgtg
     1381 tcgccagcgc tccggtcgtc gatgggggag gcctcgctcc ggctggcgcg ggcggccggg
     1441 tacgagggcg cggggacggt cgagttcctg ctcgacgacg gaggcgcgtt ctcattcctc
     1501 gaggtcaacg cgcggctcca ggtggagcac ccggtcacgg aggcggtgac cggtctcgac
     1561 ctcgtcgagc agcagctgcg cgtggcggcg ggggagccgc tcgcgttcgc ccaggccgac
     1621 gtacgtctcg acggacacgc gatggagctg cggatcgtgg ccgaggacgc cgcggccggg
     1681 ttcctgccgt cgacgggcgt cgtgacggcg tgggacatgc ccgagcgggt ccgggtggac
     1741 acgggcgtcg cgctcggctc gacggtgtcg ccgttct
//
LOCUS       DBNHXO010000567         4803 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_5159326, whole genome shotgun sequence.
ACCESSION   DBNHXO010000567 DBNHXO010000000
VERSION     DBNHXO010000567.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5159326"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="AC162A_11385"
     CDS             <1..552
                     /locus_tag="AC162A_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HIR5011490.1"
                     /translation="EEMQIELHQIQQEVGITFIYVTHDQEEALTMSHRLAVFNAGRIV
                     QIGAPAEVYERPATSFVAGFVGTSNLLAGQTATAIVGRDGTFTVRPEKIRLEDAEGGG
                     GAAATTSDAPIGDDEYAASGRIRSVVYLGPDTRFHVALDAGGELVVTQQNLATTSTEV
                     LAQQGRAVRLVWKRQHTREIAGG"
     gene            586..1920
                     /locus_tag="AC162A_11390"
     CDS             586..1920
                     /locus_tag="AC162A_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5011491.1"
                     /translation="MRRPILALVAVVALMAAACSSGATPSAAPSQAATAAPTPAPTPA
                     ASAPASASAEAPSQAAASLIPSEYGTMTPLKPAVDLSTVGGPGEGELNIIIWAGYAED
                     GSNVAEYDWVKPFTEATGCKVNTKIGNTSDEMVSLVRGGGYDGVSASGDASLRLILDG
                     NVAPIDPATIPGFGDVAPFLQDAPHYVVDGKHYGTPHGWGGNTLLYNTEEVTPAPTSW
                     DVVFDPAKAEPYAGKITAYDAPIYIADAALYLKAHRPELGITDPYELTQPQFDAAVEL
                     LKQQRPWVGKYWSAFADEIDNFTNGTTIVGTTWPYQYNTLLAGGTPVAQVVPTEGMTG
                     WADTWMLTSDATHPNCMLKWMTWMLTPEVQAQVAESFGEAPANPKACKYLDAVYASYG
                     IKDFCTGYGVNDPNFYNSIAFWKTPQVDCGTGGTEPCIDFATWTQKWTEIKG"
     gene            1985..2887
                     /locus_tag="AC162A_11395"
     CDS             1985..2887
                     /locus_tag="AC162A_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5011492.1"
                     /translation="MTSRAEGAPPQGLRRRLFAFLYRHRTLQAVLLLTPPMGWFAVVY
                     LGSLVVLFVAAFWSLDPLTSAVKQEFTLKNFQQLFTDPVYRTITLRTVAFAAVVTLFD
                     AVIAFPIAYYMARIAGPRVRAILFMLVLMPLWSSYLVRVYAWRVILAKGGPLEWFFAQ
                     LGIPGVVLYPSDIAVLIVFTYIWLPFMILPIYAGLERIPGSFLEASGDLGARPTTTFR
                     RVVFPLAFPAIVAGSIFTFSLTLGDYITPGLVSNTQFIGNVIYESQGVAGNIPFAAAY
                     ATIPVMIMAVYLLIARRFGAFDAL"
     gene            2901..3695
                     /locus_tag="AC162A_11400"
     CDS             2901..3695
                     /locus_tag="AC162A_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007035293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5011493.1"
                     /translation="MRSSHRLVGLATIAVLVFLYVPIGIIFLYAFNGDRTQTWPIDNW
                     TTHWWGDAFANESIREALLLSVEVALAATALALLLGSLASFAVHRFRFFGRNTVSFLL
                     VLPIALPGIVTGMALNATFHTADIPFGFLTIVVGHATFCVVVVYNNVIARLRRSSPNI
                     EEASMDLGADTWQTFRYVTLPVIRTALLAGGLLAFALSFDEVIVTTFTAGTQETLPIW
                     ILANLRLPNQRPLVNVVAVTLILLSAIPVYLSHRFSSDAGGPGRRI"
     gene            4067..4351
                     /locus_tag="AC162A_11405"
     CDS             4067..4351
                     /locus_tag="AC162A_11405"
                     /inference="COORDINATES: protein motif:HMM:NF013427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR5011494.1"
                     /translation="MLGELAGIVVEGPGQMTYGSQAAAATHVFEQQRDLALRDRDEQH
                     LAAIDAAIVRLDAGIYGQCESCGNPIAAERLEALPSAALCIDCQRAARRR"
     gene            4348..>4803
                     /locus_tag="AC162A_11410"
     CDS             4348..>4803
                     /locus_tag="AC162A_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR5011495.1"
                     /translation="MTEPDVALVGIDEIRAAAAILAGIALRTPLVPFGSTERNQWLKA
                     ESLQPIGAFKIRGAYNAAAALSPADRARGLITYSSGNHAQGVARAARLLGVPAAIVMP
                     SDAPAVKRARVEADGAQVVTVGTASEERRLVAEEIARTRGLAIIPPYDDP"
BASE COUNT          723 a         1765 c         1580 g          735 t
ORIGIN      
        1 gaggagatgc agatcgagct gcaccagatc cagcaggaag tcgggatcac gttcatctac
       61 gtgacccacg accaggaaga ggccctcacg atgagccacc gtctggcggt gttcaacgcc
      121 ggccggatcg tgcagatcgg ggccccggca gaggtctacg agcgtccggc gaccagcttc
      181 gttgcgggct tcgttggcac ctccaacctc cttgccggcc agacggcgac ggcgatcgtc
      241 gggcgcgatg ggacgttcac ggtccggccc gagaagatcc ggctcgagga cgccgaaggc
      301 ggtggcgggg cggcggcgac gaccagcgac gcgcccatcg gcgacgacga gtacgccgcg
      361 tccggacgga tccgctcggt cgtctacctc ggccccgaca cccgcttcca cgtcgccctc
      421 gacgcgggcg gtgagctcgt ggtcacccag cagaacctcg cgacgacctc gaccgaggtc
      481 ctcgcccaac aggggcgcgc tgtccgcctc gtgtggaagc ggcagcacac ccgggagatc
      541 gccgggggct agcccggcac cggaactgga ggaggagaac ggatcatgcg cagacccatc
      601 ctggccctgg tcgccgtcgt cgcgttgatg gcggccgcct gttcgagtgg tgcgacgccc
      661 agcgcagccc caagtcaggc cgcgaccgcg gctccgacgc cagcgccgac gccggcggcg
      721 agtgctccgg ccagcgcgtc cgcggaggcc ccgagccagg ccgccgcgag cctgatcccg
      781 agcgagtacg gcaccatgac gccgctcaag ccggcggtcg acctgagcac ggtcggcggg
      841 ccgggcgaag gcgagctgaa catcatcatc tgggccggct acgcggagga cggctccaac
      901 gtcgccgagt acgactgggt gaagccattc accgaggcca ccggctgcaa ggtcaacacc
      961 aagatcggca acacctccga cgagatggtc tccctcgtcc gcgggggtgg ctacgacggg
     1021 gtgagcgcct ccggcgacgc gtccctgcgc ctcatcctcg acgggaacgt cgccccgatc
     1081 gacccggcca cgatccccgg cttcggcgat gtcgcgccgt tcctgcagga cgcgccccac
     1141 tacgtggtcg acgggaagca ctacggcacc ccgcatggct ggggcggtaa cacgctcctc
     1201 tacaacacgg aggaggtcac gcccgccccg accagctggg acgtggtgtt cgacccggcg
     1261 aaggcagagc cctacgccgg caagatcacg gcctacgacg ccccgatcta catcgccgat
     1321 gccgccctgt atctcaaggc ccatcggccc gagctcggga tcaccgaccc gtacgagctc
     1381 acccagccgc agttcgacgc cgcggtggag ctgctcaagc agcagcggcc gtgggtcggc
     1441 aagtactggt cggcgttcgc cgacgagatc gacaacttca ccaacgggac cacgatcgta
     1501 ggcacgacct ggccctacca atacaacacc ctgctcgcgg ggggcacgcc ggtcgcccag
     1561 gtcgtgccga ccgagggcat gaccggatgg gcggacacgt ggatgctcac gtccgacgcc
     1621 acccacccga actgcatgct caagtggatg acctggatgc tgacgcccga ggtccaggcg
     1681 caggtcgccg agtccttcgg cgaggcgccc gccaacccga aggcatgcaa gtacctcgac
     1741 gccgtctacg cctcgtacgg gatcaaggac ttctgcaccg ggtacggcgt caacgacccg
     1801 aacttctaca actcgatcgc cttctggaag acgccgcagg tcgactgcgg caccggcggc
     1861 accgagccat gcatcgactt cgccacctgg acccagaagt ggacggagat caagggctga
     1921 cgggctcgcc tcgccggtcc gcgggggtga ccggacaccg gtcgcccccg tcccggccgt
     1981 ccgcgtgaca agccgcgcgg agggagcgcc gccgcagggc ctgcggcggc gcctcttcgc
     2041 gttcctctac cgccatcgca ccctccaggc ggtgctgctc ctcacgcccc cgatgggctg
     2101 gttcgccgtc gtctacctgg gctcgctcgt tgtcctgttc gtcgcggcgt tctggtcgct
     2161 cgatccgctc acctcggcgg tgaagcagga gttcacgctc aagaacttcc agcagctgtt
     2221 cacggacccg gtctaccgga cgatcacgct gcgcacggtc gccttcgcgg cggtcgtgac
     2281 cctgttcgac gcggtcattg cctttcccat cgcctactac atggcccgga tcgcgggccc
     2341 gcgggtgcgc gcgatcctgt tcatgctcgt cctgatgccg ctgtggtcga gctatctcgt
     2401 ccgcgtctat gcctggcggg tgatcctcgc caagggcggg ccgttggaat ggttcttcgc
     2461 ccagctcggg atccccgggg tcgtcctgta cccgagcgac atagcggtgc tgatcgtgtt
     2521 cacctacatc tggctgccgt tcatgatcct gccgatctac gccggcctcg agcggatccc
     2581 cggctcgttc ctcgaggcgt ccggcgacct cggggcccgc ccgaccacca ccttccgacg
     2641 cgtggtcttc ccgctggcgt tcccggccat cgtcgcgggc tcaatcttca cgttctcgct
     2701 gaccctgggc gactacatca cgcccggcct cgtgtcgaac acccagttca tcggcaacgt
     2761 gatctacgag agtcagggtg tggccggaaa catcccgttc gccgcggcct acgccacgat
     2821 cccggtcatg atcatggccg tctacctgct catcgcgcgc cgtttcggcg cgttcgatgc
     2881 gctgtgagcg aggacgcttc atgaggtcga gccaccgact ggtcgggctg gcaacgatcg
     2941 ccgtcctggt gttcctctac gtcccgatcg ggatcatctt cctgtacgcc ttcaacggcg
     3001 accgcaccca gacctggccg attgacaact ggaccacgca ctggtggggc gacgcgttcg
     3061 ccaacgagtc gatccgggag gccctgctgc tgtcggtcga ggtggcgctc gcggccaccg
     3121 ccctggcgct cttgctgggc tcgctcgcgt cgttcgcggt ccaccggttc cggttcttcg
     3181 gtcgcaacac ggtgtcgttc ctgctggtgc tgccgatcgc cctccccggc atcgtcaccg
     3241 gcatggcgct caacgcgacg ttccacaccg ccgacatccc gttcggcttc ctgacgatcg
     3301 tcgtcggcca cgcgaccttc tgcgtcgtcg tcgtctacaa caacgtcatc gcccgcctcc
     3361 gcagatcctc gcccaacatc gaagaggcgt cgatggacct gggcgcggac acgtggcaga
     3421 cgttccggta cgtgaccctg cccgtgatcc ggaccgcgct gctcgccggc gggctgctgg
     3481 cgttcgcgct gtccttcgac gaggtcatcg tcacgacctt caccgccggc acccaggaga
     3541 cgctgccgat ctggatcctg gccaacctgc ggctgccgaa ccagcgtccg ctggtcaacg
     3601 tcgtcgcggt gacgctcatc ctcctgtcgg cgatccccgt ctacctgtcg catcggttca
     3661 gcagcgacgc cggcggcccc gggcgccgaa tctagccgga cctaccagcg gcgggcgtgc
     3721 ccgcaggggg tcagcgcgtc gccgctcctt gagcgttcga cgctcaccat cgctttgccc
     3781 cctcgtatac gagcacgagc ccgagcgcga gcgcgctcgc gaacccggca acggccgcgc
     3841 ggacctgccg aaccatcgtc atcctccgcc ccgggcaccg ggactcgagc caccctcgat
     3901 ccctccgagg agtgcccgat cgggggagcg gcaccggtga ctatggcggc cgccacttac
     3961 gcgccactta tcccggcgag ccgcgccggg gcgtagcatc gagtcatgga caagccccag
     4021 acctcgttcg acccgcgcgc cgcgctccag gcggaagcgt gaacaggtgc tgggcgagct
     4081 cgcggggatc gtggtcgagg ggcccgggca gatgacctac gggtcccagg cggcggccgc
     4141 gacccacgtg ttcgagcagc agcgggacct cgcgctgcgg gaccgggacg agcagcacct
     4201 cgcggccatc gacgcggcca tcgtgcggct cgatgcgggc atatacggcc agtgcgagtc
     4261 gtgcggcaac ccgatcgcgg cggagcggct cgaggcgctg ccgtcggcgg cgctgtgcat
     4321 cgactgccag cgcgccgcgc gccggcggtg acggaacccg acgtcgccct cgtcggcatc
     4381 gacgagatcc gcgcggcggc cgcgatcctg gccgggatcg ccctgcgcac gcccctggtc
     4441 ccgttcggga gcaccgagcg gaaccagtgg ctgaaggccg aatcgctgca gccgatcggc
     4501 gcgttcaaga tccgcggcgc ctacaacgcc gcggccgcgt tgtcgcccgc cgatcgcgcc
     4561 cgcggcctca tcacctactc ctcgggcaac cacgcgcagg gtgtcgcccg ggcggcgcgg
     4621 ctgctcggcg tcccggcggc gatcgtgatg ccgtccgacg cacccgcggt caagcgcgcg
     4681 agggtcgagg cggacggcgc gcaggtcgtg accgtcggca cggcgtccga ggagcgacgc
     4741 ctcgtcgcgg aggagatcgc gcggacgcgc ggcctcgcga tcatcccgcc ctacgacgac
     4801 ccg
//
LOCUS       DBNHXO010000568         3592 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2181344, whole genome shotgun sequence.
ACCESSION   DBNHXO010000568 DBNHXO010000000
VERSION     DBNHXO010000568.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2181344"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="AC162A_11415"
     CDS             complement(<1..448)
                     /locus_tag="AC162A_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011496.1"
                     /translation="MQRPVVNLVFLALALALAACGGGTATAAPDGGGGAAPPAATDTT
                     APTNAADAPTDPASAPTDAPQGPGSAGGVCDLATVDELAGVFGTPVSTRVLAGPPDTC
                     LAETAEGDVLVSWVLATENAASVYELLTDGARPVPGIGDKAAFVEYT"
     gene            774..2393
                     /gene="pckA"
                     /locus_tag="AC162A_11420"
     CDS             774..2393
                     /gene="pckA"
                     /locus_tag="AC162A_11420"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR5011497.1"
                     /translation="MTRPAPTPRLGRNVLTNLSVAELYERAIAADEGVIAADGPLVVR
                     TGQHTGRSPRDKFIVREPWSASKVWWGEVNAEISEANYDRLRARLMGYLADRELYAQD
                     MHIGAHPDHRRSLRVYTETAWASIFARNLFRRPAREDLATFEPNFTIIDVPSFEADPA
                     TEGTRSGTAILLHLKRMEIIIVGTKYAGEIKKSAFTVMNYLMPDEGVLPMHSSINVGQ
                     AGDACIFFGLSGTGKTTLSADPLRSLIGDDEHGWGEGYSFNFEGGCYAKTIRLSPMYE
                     PDIFATTKRFGTILENVDIDPLTRSLDLDSERFTENTRGAYPLDYIGNADPTGIAPGP
                     RNIVFLTADAFGVLPPISRLTREQAAYHFISGYTAKLAGTEVGVKEPTATFSAGFGAP
                     FLPRHPGEYAAMLVDRLERFDVPVWLVNTGWTGGPYGTGERMNINHTRNMVRAAIGGA
                     LADVSTVEDPIFRVAVPVSVPGVPDELLTPRETWADPVAYDAAARRLATMFHANFAKY
                     ADGVSEAIREAGPAPVHEDLGDTAISAPGEG"
     gene            2459..3388
                     /gene="mdh"
                     /locus_tag="AC162A_11425"
     CDS             2459..3388
                     /gene="mdh"
                     /locus_tag="AC162A_11425"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR5011498.1"
                     /translation="MARNKVTVIGAGNVGATTAQRIAEAGLADVVLVDIVDGLPQGKG
                     LDLAEAAPVVGHDARVTGTNDYADTAGSDIIVVTSGLARQPGMSRDDLLAKNAGIVAS
                     VVGQAAAVSPDAILIVVTNPLDAMCHVAMRASGFPRERVLGMAGVLDSARFRTFIAQE
                     MGVSVEDVHAFVLGGHGDTMVPLSRYSTVAGVPITELLPADRVRALEERTANGGAEVV
                     ALLKTGSAFYAPAASTFEMVDAILRDRKRVLPCAVQLKGEFGVDGLFVGVPVVLGRDG
                     LERVFEITLTADERIAFDASAAAVQELVDKLPA"
BASE COUNT          533 a         1351 c         1208 g          500 t
ORIGIN      
        1 cggtgtactc gacgaacgcc gccttgtcgc caatccccgg gaccggcctc gccccgtcgg
       61 tcaggagctc gtagacgctg gccgcgttct cggtcgcgag cacccaggac accagcacgt
      121 cgccctctgc ggtctctgcc aggcacgtgt ccggcggacc agcgagcacg cgcgtcgaca
      181 cgggcgtccc gaacacgccg gccagctcgt cgaccgtggc gaggtcgcac acgccgccag
      241 ccgagccagg gccttgcggc gcatcggtgg gtgcactggc agggtcggtg ggcgcatccg
      301 ccgcgttggt tggcgcagtg gtgtcggtgg cggccggagg cgccgccccg cccccgccgt
      361 ccggggcagc ggttgccgtg ccaccgccgc aggcggccaa tgccagggcc agggccagaa
      421 agaccaggtt caccacgggt cgctgcatcg gggatacctc gctgccggtg gtgcgccggc
      481 cgacgtcagc cggcttcacc accgcggaca gcctcgcgga ccggcgactg gccgacatcg
      541 tccagttgcg ccacgtcgcg tccgcgatgg cggcaaggcc ggaccgcccg tgggaacgtg
      601 accttcggcg actggctgcc tgacggatgg cccgctacaa tcgccgaaac cgaatcgcaa
      661 ccttgactgt ggaagccgac tcccgggcat cgtgcccgtc acgcgcggcc ggtcaccgag
      721 cgatcgcggg cgccttggcg tccgcgaccc agtcgaggac acgaatcgtg acgatgacga
      781 ggcccgcccc aacaccacgg ctcggccgca acgtcctgac caatctctcc gttgccgagc
      841 tgtacgagcg cgccatcgcg gcggacgagg gcgtcatcgc cgccgacggc ccgctcgtcg
      901 tccggaccgg gcagcacacc ggccgctcgc cgcgcgacaa gttcatcgtg cgcgagccgt
      961 ggagcgcgtc caaggtctgg tggggcgagg tcaacgccga gatcagcgag gccaactacg
     1021 accggctgcg cgcccgcctg atgggctacc tcgcggaccg cgagctgtac gcccaggaca
     1081 tgcacatcgg cgcccatccc gaccaccgcc ggagcctgcg cgtgtacacc gagaccgcgt
     1141 gggccagcat cttcgcgcgc aacctgttcc gccggcccgc gcgcgaggac ctggccacct
     1201 tcgagcccaa cttcacgatc atcgacgtgc ccagcttcga ggccgacccg gctaccgagg
     1261 gcacgcgatc cggaacggcg atcctgctcc acctcaagcg gatggagatc atcatcgtcg
     1321 gcacgaagta cgcgggcgag atcaagaagt cggcgttcac ggtcatgaac tacctgatgc
     1381 ccgacgaggg cgtgctgccc atgcactcct cgatcaacgt cggccaggcc ggcgacgcgt
     1441 gcatcttctt cggcctcagc gggaccggca agacgaccct gtccgccgac ccgctgcgaa
     1501 gcctgatcgg cgacgacgag cacggctggg gcgagggcta cagcttcaac ttcgagggcg
     1561 gctgctacgc caagaccatc cgcctgtcac cgatgtacga gcccgacatc ttcgcgacca
     1621 cgaagcgctt cgggaccatc ctcgagaacg tcgacatcga ccccctcacc cgctccctcg
     1681 acctcgactc ggagcggttc accgagaaca cccgtggcgc ctatccgctc gactacatcg
     1741 gcaacgccga ccctacgggc atcgcgccgg ggccccggaa catcgtgttc ctgaccgccg
     1801 acgcgttcgg cgtcctgccc ccgatctcgc ggctgacgcg cgagcaggcc gcctatcact
     1861 tcatcagcgg ctacacggcc aagctcgccg gcaccgaggt tggcgtcaag gagccgacgg
     1921 cgaccttctc ggccggcttc ggggccccgt tcctgccgcg ccatcccggc gagtacgccg
     1981 cgatgctggt cgaccgcctc gagcggttcg acgtcccggt ctggctggtg aacaccggct
     2041 ggacgggcgg gccgtacggc accggcgagc ggatgaacat caaccacacc cggaacatgg
     2101 tccgggccgc catcggcgga gccctggccg acgtctcgac ggtcgaggat ccgatcttcc
     2161 gggtcgccgt cccggtcagc gtcccgggcg tccccgacga gctgctgacg ccgcgcgaga
     2221 cgtgggccga ccccgtggcc tacgatgcgg cggctcgcag gctggccacg atgttccacg
     2281 ccaacttcgc gaagtacgcc gacggcgtca gcgaggcgat ccgcgaagcc gggccggcgc
     2341 ccgtccacga ggacctcggc gacaccgcca tctccgcgcc gggcgagggc tgaccggccg
     2401 tcccgcaccg ctcacacaac cgaatccgtc ccggcagctc ctggagggtc cgcgcgtcat
     2461 ggctcgcaac aaggtcaccg tcatcggcgc cgggaacgtc ggcgccacca ccgcccagcg
     2521 aatcgccgag gccggcctcg ccgacgttgt cctcgtcgac atcgtcgacg ggctcccgca
     2581 gggcaagggt ctcgatctcg cggaggccgc tcccgtcgtg ggccacgatg cgcgggtcac
     2641 cggcaccaac gactacgccg acaccgccgg gtccgacatc atcgtcgtga cgtcgggcct
     2701 cgcgcgccag cccggcatga gccgcgacga cctgctggcc aagaacgcgg ggatcgtcgc
     2761 gagcgtcgtg gggcaggcgg ccgcggtgtc gcccgacgcg atcctgatcg tcgtcaccaa
     2821 cccgctcgac gcgatgtgcc acgtcgcgat gcgggcctcc ggcttccccc gggaacgcgt
     2881 cctgggcatg gccggcgtgc tcgactccgc gcgcttccgc accttcatcg cccaggagat
     2941 gggcgtgtcg gtcgaggacg tccacgcgtt cgtgctcggc ggccacggcg acacgatggt
     3001 gccgctctcg cgctactcga cggtcgcggg cgtgccgatc acggagctcc tgccggcgga
     3061 cagggttcgc gcgctcgagg agcggacggc gaacggcggc gccgaggtcg tcgcgctgct
     3121 caagacaggc tcggccttct acgccccggc cgcgtcgacg ttcgagatgg tcgacgccat
     3181 cctgcgcgac cggaagcgcg tactcccgtg cgcggtgcag ctcaagggcg agttcggcgt
     3241 cgacggcctg ttcgtcggcg tgccggtcgt cctcggccga gacgggctcg agcgggtgtt
     3301 cgagatcacc ctgacggcgg acgagcggat cgccttcgac gcttccgccg ccgcggtcca
     3361 ggagctcgtc gacaagctgc ccgcctgagg ggttcgtcgc tccgcggggt ccttcgagcg
     3421 cgtggtgcgc ctgcgcaccc cctgtccctc ggcgactcgc gcaatcgcgt tgacccctgc
     3481 tcgataccgg ggtacgttca cgccatcggg caggtccgcc tggaccgacc ccagcgcgcc
     3541 gccagagccg gggggccagc accctgcacc ccctgtccgt ggggtttgac cg
//
LOCUS       DBNHXO010000569         2597 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_2776390, whole genome shotgun sequence.
ACCESSION   DBNHXO010000569 DBNHXO010000000
VERSION     DBNHXO010000569.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2776390"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="AC162A_11430"
     CDS             complement(<1..1276)
                     /locus_tag="AC162A_11430"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HIR5011499.1"
                     /translation="MRVPRAPLLLLFVLSGAAGLIYEVVWARQLVLVFGNTSQAVSTI
                     LTGFFGGLAIGGFFGGRFADRVARPLRMYGLLELILVVIVVLTPLSFRLIGEVYRGVY
                     PALATAPIALALVRFLLAILALAPATVLMGATLPTLTRFLSTGTAGIGRAFQQLYAAN
                     TIGAILGTAVAGFALIELFGLTGALLVGAACSGTAGIVALILDRRVGAPVEAPVEAPV
                     AVAASVAPVAARATPDRRRLALTLAFVSGLTSLGYQVVWNRLLGSGTGSSTYVFTVIL
                     VLFLIGIAVGAILLGVIRPRIRSVVGLIAVAQLLTAALVTLGAAVLASPTDPFLNGAS
                     AKFLDGLRDFAWSTAFVVLPPTIIMGLTFPATAALLGDETGSEGSASGALLAVNTTGA
                     IVATFVLPFFVIPLIGSPATLALLAIVNVAVGA"
     gene            complement(1287..2186)
                     /locus_tag="AC162A_11435"
     CDS             complement(1287..2186)
                     /locus_tag="AC162A_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011500.1"
                     /translation="MLLAWWVSGRIGAGRIRDRLAMAVLLGFSTQIWWVTTRGGVWHT
                     GHLIATILSLLLLAEMFGRRRALLMGLLIGAAFLTRAPIAFITPAVALWLVASWSDVL
                     GGGRSIGERIRALPWRGWILLGIGLLPALAFFFWYNIDRFGNPLESGYAMATLPEWLE
                     AIRRQGLFSTSHVQMNMEYLFWKMPAFTNTFPYFRPDGLGMSVLLTSPALLLAIRAPW
                     RDGRSWLLLFGAIAVLIPTLLYYGGGWLQYGYRYFLDSIPFIWALVAMGIASRGSLKW
                     WGWLLIGWGVLMGLGGVYWAYHF"
     gene            complement(2361..2504)
                     /locus_tag="AC162A_11440"
     CDS             complement(2361..2504)
                     /locus_tag="AC162A_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011501.1"
                     /translation="MDRVRSPVVWAGVLLAIASFFVYWLSNRAFDATRGDLFYLADAF
                     LQG"
BASE COUNT          503 a          884 c          951 g          259 t
ORIGIN      
        1 acgcgccgac ggcgacgttg acgatcgcga ggagggcgag cgtcgcgggc gacccgatca
       61 gggggatgac gaagaacggg agcacgaagg tcgccacgat cgcgcccgtc gtgttgacgg
      121 cgaggagcgc gcccgaggcc gatccctcgg accccgtctc gtcgccgagg agcgcggccg
      181 tcgcggggaa cgtgaggccc atgatgatcg tgggtggcag gacgacgaag gcggtggacc
      241 aggcgaagtc ccgtaggcca tccaggaact tggccgaggc cccgttgagg aacgggtctg
      301 tcggcgaagc gagcaccgcg gcgcccagcg tcaccagcgc cgccgtcagc agctgggcga
      361 cggcgatcag cccgaccacc gagcggatgc gcggccggat cacgcccagc aggatcgccc
      421 cgaccgcgat gccgatcagg aacaggacca ggatgaccgt gaacacgtac gtcgagctgc
      481 cggtgcccga tccgaggagc cggttccaga cgacctggta gcccagcgac gtcaggcccg
      541 agacgaacgc gagcgtcagg gcgagccgac ggcggtcggg cgtcgcccgg gcggcaaccg
      601 gggcgacgga agccgcgacg gccaccggcg cctcgaccgg cgcctcgacc ggcgccccca
      661 cccgccggtc caggatcagc gccacgatgc cggccgtccc cgaacaggcc gcccccacca
      721 ggagcgcccc cgtgaggccg aacagctcga tgagcgcgaa cccggcgacc gccgtgccca
      781 ggatcgcgcc gatggtgttc gccgcgtaca gctgctggaa cgcgcgcccg atgccggcgg
      841 tccccgtcga gaggaagcgc gtcagcgtcg gcagggtcgc gcccatgagc acggtcgcgg
      901 gcgcgagggc gaggatcgcg agcaggaagc gcacgagcgc gagggcgatc ggggccgtag
      961 cgagcgccgg gtagacgccc cggtagacct cgccgatcag gcggaagctg agcggcgtga
     1021 gcacgacgat gacgacgagg atgagctcga gcaacccgta catccggagc ggcctggcga
     1081 cccggtccgc gaaccgcccg ccgaagaagc cgccgatcgc gagcccaccg aagaacccgg
     1141 tgaggatcgt cgacaccgcc tggctcgtgt tcccgaacac gaggacgagc tggcgtgccc
     1201 aaaccacctc gtagatcagg ccggccgcgc cggacagcac gaacagcagc aggagcggcg
     1261 cccggggaac cctcatcgac acatcctcaa aagtggtacg cccagtacac gccgccgagg
     1321 cccatcagca cgccccagcc gatcaggagc caaccccacc acttgagcga cccacggctc
     1381 gcgatgccca tggcgacgag cgcccagatg aaggggatcg agtccaggaa gtagcggtag
     1441 ccgtactgga gccagccgcc gccgtagtag aggagcgtcg ggatcaggac cgcgatggcc
     1501 ccgaacagca gcagccacga gcggccgtcg cgccacgggg cgcggatcgc gagcagcagc
     1561 gccgggctgg tgagcagcac ggacatcccc aggccgtcgg ggcggaagta cgggaaggtg
     1621 ttggtgaacg ccggcatctt ccagaacagg tactccatgt tcatctgcac gtgggacgtc
     1681 gagaacaggc cctgccggcg gatcgcctcc agccactcgg gcagcgtggc catcgcgtag
     1741 ccggactcga gcgggttgcc gaagcggtcg atgttgtacc agaagaagaa cgcgagggcg
     1801 ggcaggaggc cgatgccgag caggatccag ccccgccagg ggagcgcgcg gatgcgctcg
     1861 ccgatcgacc ggccaccgcc gaggacgtcc gaccacgacg ccaccagcca cagggcgacg
     1921 gccggcgtga tgaaggcgat cggggcccgg gtcaggaacg cggcaccgat cagcagcccc
     1981 atcagcagcg cgcgcctgcg gccgaacatc tcggccagta gcaagagcga aaggatcgtc
     2041 gcgatcaggt gaccggtgtg ccagacgccg ccgcgagtcg tgacccacca gatctgggtc
     2101 gagaatccga gcaggaccgc catcgccagc cggtcgcgga tcctgccggc accgatccgg
     2161 cccgagaccc accacgcgag cagcacgacc gtcgcggcga ggaacgcgtt gatcccggtc
     2221 tcccactggt ccgcgacggc ggggccggtc agcgcgacga tcggggcgag gacgagggcc
     2281 gggaacgggg cgaacgggac gaagatcagt cccttgtagt agatgacgtc gaagaagcca
     2341 agagcgacgg cgatgttcgt ctagccctgc aggaacgcgt cggcgaggta gaacaggtcg
     2401 ccgcgcgtcg cgtcgaaggc gcgattcgac agccagtaca cgaagaacga ggcgatcgcc
     2461 agcaggacgc cggcccagac gaccggcgaa cggactcggt ccatggactc cactgggtga
     2521 ggggtgcggc gcgtagggct cgcgcgggac gtcggcaagg atagcgggac tcgcccgcgt
     2581 cgcccgcgtc gcccgcg
//
LOCUS       DBNHXO010000570         2101 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4761865, whole genome shotgun sequence.
ACCESSION   DBNHXO010000570 DBNHXO010000000
VERSION     DBNHXO010000570.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4761865"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..450
                     /locus_tag="AC162A_11445"
     CDS             100..450
                     /locus_tag="AC162A_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011502.1"
                     /translation="MGVSITRGLIGGIGVVLVVVGFVVAAAAPGGGVVSFLFLIVPGV
                     ILIIAALIERLRYRSLAAEGLGDTHGPGGGEPDVPEPRFRPTDERFVDPTTGVQMRVF
                     VDPRTGERRYLAEG"
     gene            610..>2101
                     /gene="ppk1"
                     /locus_tag="AC162A_11450"
     CDS             610..>2101
                     /gene="ppk1"
                     /locus_tag="AC162A_11450"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR5011503.1"
                     /translation="MTAVRPAPGPRTATRGRRRAARDQRLPILNRELSWLEFNARVLH
                     EAGDERNRLLDRVRSLFFFVNNLDEFFQVRVSGLRRQELSGSQAISADGRTADEQLDA
                     IRARVLELMAEQARIFAALRLHLETEGIEILDYDDVPEHHEALRHRFHDEIFPVLTPL
                     AVDPGHPFPYISTLTLSIAVGLRDPETGEERFARVKIPPLLPRLVELEPGRFVLLDQV
                     IEANLDELFRGMEILETHLFRVTRDADIAIEEDEADDLLLAIEEEIRRRRFGEAVRLE
                     VERSMPELTRRILSKGIGVRDDDVYEVAGMLDLTALWQLVDLDRPDLKSPPYVPVVPA
                     RLMPHDEDEQVDVFAQIRGGDMLVHHPYESFSASVERFIAQAADDPEVLTIKQTLYRT
                     SGDSPIVQNLIRAAERGKQVVVLVEIKARFDEEANIVWARRLEQAGAHVVYGLVGLKT
                     HSKVALVVRREGGGLRRYVHIGTGNYNSKTARSYVDLGILSCREELG"
BASE COUNT          306 a          748 c          729 g          318 t
ORIGIN      
        1 tcggagctgg ccgggaccat gtccgagatc gtcgcctacg ggacggccgt ggtcgtcatg
       61 cccgacgccg gcgcggccca ggcgcaggag tagcgcgcga tgggcgtatc gatcacccgg
      121 ggactgatcg gtggcatcgg cgtcgtgctg gtcgtggtcg gcttcgtcgt cgcggccgcg
      181 gccccgggcg gtggcgtggt ctcgttcctg ttcctgatcg tgccgggagt gatcctgatc
      241 atcgcggcac tcatcgagcg gctgcgctac cggtcgctcg cggccgaggg tctgggcgac
      301 acgcacggcc cgggcggtgg cgaacccgac gtcccggaac cccgcttccg cccgaccgac
      361 gagcgcttcg tcgacccgac cacgggcgtg cagatgcggg tgttcgtgga cccccggacc
      421 ggagagcggc ggtacctcgc ggagggctga ccgcaggccc gcaactcatg gggtacggac
      481 gcaccgatag gacctcctcc ccatcggccg ggctcgggcg gcgtggtatt ctcggcggcc
      541 ccgaccggga tcgggccgtc ggtgcgttcg cgccccgcgc ccgggctcgg atcgcaccgt
      601 gtgaggacca tgaccgccgt tcgcccagca cccggaccgc gtaccgcaac ccgcggacgc
      661 cgacgggccg cgcgggacca gcggctcccc atcctgaacc gcgagctctc gtggctcgag
      721 ttcaacgcgc gcgtgctcca cgaggccggg gatgagcgca accggctgct cgatcgtgtc
      781 cggtcgctgt tcttcttcgt caacaatctc gacgagttct tccaggtccg ggtgtccggc
      841 ctgcgccgtc aggagctgag cggctcgcag gcgatctccg ctgacggccg caccgccgac
      901 gaacagctgg acgccatccg ggcgcgggtc ctcgagctca tggccgaaca ggcgcggatc
      961 ttcgcggccc tgcgcctcca cctcgagacc gagggaatcg agatcctcga ctacgacgac
     1021 gtcccggagc atcacgaggc cctgcgccat cgcttccacg acgagatctt tccggtcctg
     1081 acgccgctgg ccgtcgaccc cggtcacccg ttcccgtaca tcagcacgct cacgctctcg
     1141 atcgccgtgg ggctccgcga cccggagacc ggcgaggagc gcttcgcccg ggtcaagatc
     1201 ccgccgctgc tgccccgcct cgtcgagctg gagcccggac ggttcgtgct gctcgaccag
     1261 gtcatcgagg cgaacctcga cgagctgttc cggggcatgg agatcctcga gacccacctg
     1321 ttccgcgtga cgcgcgacgc ggacatcgcg atcgaggagg acgaggccga cgatctcctg
     1381 ctcgcgatcg aagaggagat ccggcgccgg cgcttcggcg aggccgtgcg gctcgaggtc
     1441 gaacgctcga tgccggagct cacccgccgg atcctcagca agggaatcgg cgtccgggat
     1501 gacgacgtct acgaggtcgc cgggatgctc gacctcaccg cgctgtggca gctggtcgac
     1561 ctcgatcgcc cggacctcaa gtccccgccc tacgtccccg tcgtgccggc gcggctgatg
     1621 ccccacgacg aggacgagca ggtcgacgtg ttcgcccaga tccggggcgg cgacatgctc
     1681 gtgcaccacc cgtacgagtc gttctcggcg tccgtggagc ggttcatcgc gcaggccgcc
     1741 gacgacccgg aagtcctcac gatcaagcag acgctctacc gcacatcggg cgactcgccg
     1801 atcgtgcaga acctgatccg cgcggcggag cgcggcaagc aggtggtggt gctggtcgag
     1861 atcaaggcgc gcttcgatga ggaggccaac atcgtgtggg cccgacgcct cgagcaggcg
     1921 ggtgcccacg tcgtgtacgg actggtaggc ctgaagacgc actccaaggt cgcgctcgtc
     1981 gtgcgtcgcg agggcggcgg gctgcggcgc tacgtgcaca tcgggaccgg caactacaac
     2041 tccaagaccg cccgctcgta cgtcgacctc gggatcctgt cctgccgcga ggagctgggc
     2101 g
//
LOCUS       DBNHXO010000571         4002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_3570268, whole genome shotgun sequence.
ACCESSION   DBNHXO010000571 DBNHXO010000000
VERSION     DBNHXO010000571.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3570268"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1534)
                     /locus_tag="AC162A_11455"
     CDS             complement(71..1534)
                     /locus_tag="AC162A_11455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5011504.1"
                     /translation="MVGIRTRLAITLVALVALTVAAIGVGVYAFVESSLRERMLADAR
                     QQADFNLSVLLPAEVPRPTDSTTFAASGLPEAFALRGDVDTIADFGDGNPYTPGRLLG
                     GLNELSPELRAIVGNGELGFAWQTLQDEPVLVVGGRQGGPPDLYFVFSAAAVETALAQ
                     LRIGLLAAGLLAVLVALATAGLIARRILRPVDEAVLAARRIAAGDLTARVPEGGRDEF
                     GRWAAEFNRMAGTLESTVGRLESAQQQNRRFVADVSHELRTPLTALVAEASLIEAGMD
                     GLSPDGRRAAELLVADVRRLRTLVDELMEVSRFDADAERVSLEPVDLGRVVATVVAAR
                     LPTAAVALPARPVVVDTEPRRLDRILGNLVDNARDHAPGSPVEVSLTRTAAGAVIVVA
                     DRGPGVARDDLPHVFDRFYKADPSRTGGSSGLGLAIAAEHAALLGATLRARPRPGGGL
                     IFALTLPVTQPLPGGEGPDMAVRDDDRESQPAPGSVT"
     gene            complement(1568..2275)
                     /locus_tag="AC162A_11460"
     CDS             complement(1568..2275)
                     /locus_tag="AC162A_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5011505.1"
                     /translation="MDAHILLVEDDPSIREVTALGLRAAGFTVSTAEDGVDGLERWRA
                     DRPDLLLLDVMLPRMDGLEVCRAIRHESTIPIVMLTARADTIDVVVGLESGADDYVRK
                     PFEMPELVARVRAALRRRTGGLGPDDANGAEPAISLGDLDIDLAGRTVERDGRDIPLT
                     RTEFDLLVELARHPGQVFTRDVLLDHVWGYDYLGDSRLVDVAVGRLRAKVEPDPSNPA
                     LVLTVRGAGYKAARPAA"
     gene            complement(2295..2681)
                     /locus_tag="AC162A_11465"
     CDS             complement(2295..2681)
                     /locus_tag="AC162A_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005242597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HIR5011506.1"
                     /translation="MDDRDDELFEGWVIEAIDALPAVFRDQLGSVAITIEAWPSQAQL
                     ESTGAPGLYGLYQGVPRSTYGADGAAIPSRITIFRGTIERHFPDDASRRAQVIDTVHH
                     EIAHHFGISDARLHELAAGPGDGSRP"
     gene            2754..>4002
                     /gene="dnaE"
                     /locus_tag="AC162A_11470"
     CDS             2754..>4002
                     /gene="dnaE"
                     /locus_tag="AC162A_11470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR5011507.1"
                     /translation="MTIAPNDFVHLHTHSEFSLLDGLGRITDLVDTSVRHGFDALAVT
                     DHGALYGSIAFYQAATKAGIKPILGVETYVARRGMTDRDPRADAQPYHLILLATDLTG
                     YRNLCRLLTDAHIDGFYYKPRIDRAHLARYSEGLVGLSACLGGEIPKALEAEDWDLAR
                     TVAGEYRDILGKDRFFLELQDHGIPEQKRLNEQLLRLAPEMGLPLVVTNDLHYVHQAQ
                     HEAHDVLLCVGTGNNLDTPNRMRFETRDFYLKTAAEMAALFPDQLEAIRNTRRIAEMV
                     DLSLPLGELRIPHFPVPDGETVETWLRKECEAGLVRRYGGITPALQERLDYELGVIIS
                     MGYAGYFLIVADFVRFAREQGIATTCRGSAPGSIVTYTLGITPVDPIDYGLPFERFLN
                     PDRVTMPDIDVDFEDERRDEVINY"
BASE COUNT          606 a         1430 c         1424 g          542 t
ORIGIN      
        1 gacgggctgc acgcggccgc gaggatgccg atcacggcga gcaggatgat gcttgacctt
       61 gactggcgac tcacgtgacg gaccccggtg cgggctgtga ctcgcgatca tcgtcgcgca
      121 ccgccatgtc cggtccttcg cccccaggta acggttgggt caccgggagg gtgagggcga
      181 agatgagccc gccgcccggc ctcgggcggg cccggagggt cgcgccgagc agtgccgcat
      241 gctcggcggc aatggcaaga cccagcccgg aactgccgcc ggtccgcgac gggtccgcct
      301 tgtagaagcg gtcgaagacg tgggggaggt cgtcccgcgc gacgccgggg cctcggtcgg
      361 caacgacgat gacggcgccc gctgctgtcc gcgtcagcga cacctcgacc ggcgagccgg
      421 gcgcgtggtc tcgcgcgttg tccaccaggt tgcccaggat cctgtccagg cggcgaggtt
      481 ccgtatccac gacgaccgga cgggccggga gggcgaccgc ggccgttggc aggcgtgccg
      541 cgaccacggt ggccaccact cggccgaggt cgacgggctc caggctgacc cgctcggcgt
      601 ccgcgtcgaa gcgcgagacc tccatcagct cgtcgaccag cgtccggagc cggcggacat
      661 cggccacaag cagctccgcc gcccgccgcc cgtcgggcga gaggccgtcc atgccggcct
      721 cgatcagcga cgcctcggcg acgagggcgg tcagcggcgt tcgcagctcg tgggagacgt
      781 cggcgacgaa gcgccggttc tgctgctgag cggactcgag ccgtcccacc gtcgactcga
      841 gcgtgcccgc catgcggttg aactccgccg cccagcgccc gaattcgtcg cgcccgccct
      901 ccgggacgcg cgccgtgagg tcgccggccg cgattcgcct cgcggcaagg accgcctcgt
      961 cgaccggccg caggatgcga cgggcgatca accccgccgt cgccagggcg acgaggaccg
     1021 cgagcagacc cgccgcgagg aggccgatgc ggagctgggc gagggccgtc tcaacggcgg
     1081 ccgcggagaa cacgaagtac aggtcgggtg gtccaccctg ccggccgccg acgacgagca
     1141 cgggctcgtc ctggagcgtc tgccaggcaa agccgagctc gccattgccg acgatggccc
     1201 gcagctcggg cgacagctcg ttcaggcccc cgagcagtcg tccgggcgtg tacggattgc
     1261 cgtcgccgaa gtccgcgatc gtgtcgacgt cgccgcgcag cgcgaacgcc tcgggaagtc
     1321 cgctcgcggc gaacgtcgtc gagtccgtcg gccgcgggac ctcggccggg aggagcaccg
     1381 acaggttgaa gtcggcctgc tggcgcgcgt cggcgagcat ccgttcgcgc aacgaggact
     1441 cgacgaacgc gtagacaccg acaccgatcg cggcgacggt gagggcgacc agcgcgacca
     1501 gcgtgatcgc gaggcgcgtg cggatgccga ccatgggtcc agcctcgtcc cgtgcgtcgg
     1561 accgcggtca ggcggcgggg cgcgcggcct tgtagccggc accccgaacg gtcaggacga
     1621 gcgccgggtt cgagggatcc ggctcgacct tggctcgcag gcggccgacg gcgacgtcca
     1681 cgagtctgga gtcgccgagg tagtcgtagc cccagacatg gtcgagcagc acgtcccggg
     1741 tgaacacctg gcccgggtgg cgggcgagct cgacgagcag gtcgaactcc gtccgcgtca
     1801 gcgggatgtc gcggccatca cgctccaccg tgcggcccgc gaggtcgatg tcgaggtcgc
     1861 ccagggagat cgcgggctcg gctccgttgg cgtcgtccgg cccgaggcca ccggtcctgc
     1921 gccgaagggc cgcgcggacc cgggcgacca gctccggcat ctcgaacggc ttgcggacgt
     1981 agtcgtcggc gcccgactcg aggccgacca cgacatcgat ggtgtcggcg cgcgcggtga
     2041 gcatcacgat cggaatcgtg gactcgtgtc ggatcgcccg gcagacctcg agcccgtcca
     2101 tgcgcggcag catcacgtcg agcagcagga gatccgggcg gtcggcccgc cagcgctcga
     2161 ggccgtccac gccatcctcg gccgtcgaga ccgtgaaccc ggcggccctg aggccgaggg
     2221 cggtcacctc gcggatggaa gggtcgtcct cgacgagcag gatgtgggcg tccacgcgcg
     2281 gagtatggca gcgctcaggg gcgcgagccg tcgcccgggc cggccgcgag ctcgtgcagt
     2341 cgggcgtcgg agatgccgaa gtggtgggcg atctcgtgat gcaccgtgtc gatgacctgt
     2401 gcccgccgtg aggcgtcgtc tggaaagtgc cgctcgatcg tcccgcgaaa gatcgtgatc
     2461 cgcgacggga tcgctgcgcc gtccgcaccg tacgtcgacc gcggcacgcc ctggtacagg
     2521 ccgtacagtc ccggcgcgcc ggtcgactcg agctgcgcct gcgagggcca ggcctcgatg
     2581 gtgatcgcga cgctgccgag ctggtcgcgg aagacggccg gaagggcatc gatcgcctcg
     2641 atcacccagc cttcgaagag ttcgtcgtcg cggtcatcca tggggctgcg aagcgtagcc
     2701 ggggtccgcc gagcgtgccg tcgggcggcc accccggcta ccattgaccc cccgtgacca
     2761 tcgctccgaa cgacttcgtc cacctccaca cccactcgga gttcagcctg ctcgacgggc
     2821 tggggcggat cacggacctc gtcgatacct cggtccggca cggcttcgat gccctcgcgg
     2881 tcaccgacca cggcgcgctg tacgggtcga tcgcgttcta ccaggcggcg accaaggcgg
     2941 gcatcaagcc gatcctgggc gtcgagacct acgtagcccg ccgcggcatg accgatcgcg
     3001 acccgcgggc cgacgcgcag ccctaccacc tgatcctgct ggcgacggac ctcaccggtt
     3061 accggaacct gtgccggctg ctcaccgacg cccacatcga cggcttctac tacaagccgc
     3121 ggatcgaccg cgcgcacctc gcccgctaca gcgaaggcct cgtcggcctc tccgcgtgcc
     3181 tcggcggcga gatccccaag gccctcgagg cggaggactg ggacctcgcg cgcacggtcg
     3241 cgggcgagta ccgcgacatc ctgggcaagg accggttctt cctcgagctc caggaccacg
     3301 gcatcccgga gcagaagcgg ctcaacgagc agctgctccg gctggcgccc gagatgggcc
     3361 tcccgctggt ggtgacgaac gacctccact acgtccacca ggcgcagcac gaggcccacg
     3421 acgtgctgct gtgcgtgggc acggggaaca acctcgacac gcccaaccgg atgcgcttcg
     3481 agacccgcga cttctacctc aagaccgcgg ccgagatggc ggccctgttc cccgatcagc
     3541 tcgaggcgat tcgcaacacg cgccggattg ccgagatggt cgacctgtcg ctgcccctgg
     3601 gcgagttgcg catcccgcac ttccccgtgc ccgatggcga gacggtcgag acgtggctgc
     3661 gcaaggagtg cgaggcgggc ctcgtccgtc gctacggggg gatcacgccg gcgctgcagg
     3721 agcgcctgga ctacgagctg ggcgtgatca tctcgatggg ctacgcgggc tacttcctga
     3781 tcgtggcgga cttcgtgcgg ttcgcccgcg agcaggggat cgccacgacc tgccgcggct
     3841 ccgcgcccgg atcgatcgtg acctacaccc tcgggatcac ccccgtcgac cccatcgact
     3901 acggcctgcc gttcgagcgc ttcctcaacc ccgaccgggt gacgatgccc gacatcgacg
     3961 tggacttcga ggacgagcgc cgggacgagg tcatcaacta cg
//
LOCUS       DBNHXO010000572         5241 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4365575, whole genome shotgun sequence.
ACCESSION   DBNHXO010000572 DBNHXO010000000
VERSION     DBNHXO010000572.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4365575"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /locus_tag="AC162A_11475"
     CDS             <1..1382
                     /locus_tag="AC162A_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gluzincin family metallopeptidase"
                     /protein_id="HIR5011508.1"
                     /translation="TADVPATASTAVTAPHDLVSASANVTSVPVPNPIEPAATAEAGT
                     ATAIVPGAVHRTSLTIDATYDVDVRLKVAARRLKGTVTIDARNDSGDGIDRLMLNTVM
                     ARLGALDLGAVTVDGSRVKARIDDQTIMVPLGGVLPDGASATIVVPFAARLRANLSGS
                     NWLFTRANGIVDMHRWIPWISRKRPFNRPNHGDPFVTPVSDRVTVRLRTDVPLRFGTS
                     GHVVSASSDGLTRTFRATNVRDFVVTAAADYRVLKRRVGDTKVRLIARPGFPAARALD
                     AAANAVRKLEAKLGPYPYATLRVVQSAGAYGMEGPGIAWIPTGTPSANLTYLVTHEIA
                     HQWFYGLVGNDQAREPFADEAATDFVARRVLGLRRGSRCASDDLDRSIYRYSSLCYYE
                     VVYIQGGNVLDNARQKMGSKPFWAALRGYVADHRWGLSHTRTLLDALDAATPLNLRPT
                     WRHRFPSLY"
     gene            1385..1954
                     /locus_tag="AC162A_11480"
     CDS             1385..1954
                     /locus_tag="AC162A_11480"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5011509.1"
                     /translation="MAPITVRLATVADGAACAAIYGPFVAGTSISFELNPPTAEEMAA
                     RIAKATERTPWLVAEVDGIVRGYAYATRHRDRAAYDWTVESAVYVDGAFAGRGIGRTA
                     MTALLEVLRLQGFHLVVAGITQPNPASTSLHLALGFERIGQFEAIGWKQGRWHGVEWY
                     GLELGGRDTAPTPIRPLAEAITAWDGRER"
     gene            complement(1951..2913)
                     /locus_tag="AC162A_11485"
     CDS             complement(1951..2913)
                     /locus_tag="AC162A_11485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HIR5011510.1"
                     /translation="MGGRAAAERPLTPPFGDDADLERAGLYGPRSEAWRLNREAALLL
                     GAGPRALLLQIAHPLVAEGVDQHSDFRADPWARLAGTLRSYLRIVYGTRTAAHAEIRR
                     LNGLHRTIGGPVRDAAAAARFGAAYDARDPGLALWVHATLVDSTLATGEAWLGPIPPA
                     VRAGFYAETMPVARLFGIAESQIPADVDAFDAYVASMLAPSGPVHPSATARDLASVIL
                     HPPLAPAVEAGPVAALLGDWAGPTAALLREIPTPAYDWLLVPAIGLLPPGLRDEYGFA
                     WGARERVISAWLTTTWGFWRRVLPPGWRWFPQALAADRRIAGGS"
     gene            complement(2880..3524)
                     /locus_tag="AC162A_11490"
     CDS             complement(2880..3524)
                     /locus_tag="AC162A_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR5011511.1"
                     /translation="MSYALPSITRVPAAEKGLTIADFLVPVGLGERVSPRLRNVVLVI
                     AGAVLIYLTAQVFFTIPGNPVPYTLQTFGVLVVGGALGLRRGGLAAFLYVALGVIGLP
                     FFAEHKGGIQVIWGATGGYLVGFILAGALVGRLAELGWDRRIAGAIGATALGTLVIYL
                     VGVPWLAVTTGMSLGQAIETGMLPFLVVDTVKLLAAAAVFPVAWWVVGRRPSDR"
     gene            complement(3613..>5241)
                     /locus_tag="AC162A_11495"
     CDS             complement(3613..>5241)
                     /locus_tag="AC162A_11495"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR5011512.1"
                     /translation="TRAQVGRIVVTQGLLLGALGSALGIVAGALLAAVAATWLRTAGG
                     VTLDGPVVTPWAIVAGIAAGMIVALVAAVEPARRAGAVSPVEALRARSDAAAGVRAHA
                     GWLLVVMAAVGVLAAVLVPGIASSGALSQRTIVVYVVLLAAVLATPALLGPLGRVAGF
                     PFARVLRLEERLARAAIARDRGRTTITVGALVVGLAMVVALGVVAVNARTAATAWLDD
                     VVPGDEVLTAIAPAPVGADGYESEIAAIDGVARVTPMAAFDLSFEGTRMPAVAIRGAD
                     LDADGRLTLLSGDRATALAAIDAGGSVILPAADARGMGVEVGAVIVVATAAGQVELAV
                     AGVVERSFPGASGEAALVGWGDALDRFGVLGADAFIVRYAAGRAATAMPAVDDLARQL
                     ALSPAPVARVAGTVGDALDRVFGLLDLLALAAVVIAGFGIANTLSMDSWERSRELGVL
                     RAAGMSRRQVWRSVLVEAGILGVIGGAVGVVTGLVVGTLLLAFAGGLPSMVQIPWVTI
                     GVSLAMAVGLAMLAAAQPARIAGRRSIVAAVRGA"
BASE COUNT          816 a         1980 c         1860 g          585 t
ORIGIN      
        1 caacggctga cgtgcccgcg accgcgtcga cggcggtcac cgcgccacac gatctcgtgt
       61 cggcgtccgc gaacgtgacc tcggtacccg tgccgaaccc gatcgagccg gccgcgacag
      121 ccgaggcagg gaccgcaacg gcgatcgttc ccggcgccgt tcatcggacg agcctcacga
      181 tcgacgcgac gtacgacgtc gacgtccgcc tcaaggtcgc cgcccgacgg ctcaaaggca
      241 cggtcacgat cgacgcccgg aacgactccg gcgacggcat cgaccgactc atgctcaaca
      301 ccgtcatggc gcgcctgggc gcgctcgacc tgggcgccgt caccgtcgac gggtcaaggg
      361 tcaaggcacg catcgacgac cagacgatca tggtcccgct cggcggcgtg ctcccggacg
      421 gcgcctcggc cacgatcgtc gtccccttcg ccgcccgcct gcgtgccaac ctgagcggtt
      481 caaactggct gtttacccgg gccaacggca tcgtcgacat gcatcgctgg atcccgtgga
      541 tcagccgcaa gcgcccgttc aaccggccca accacggcga tccattcgtg accccggtca
      601 gcgatcgcgt caccgtgcgc ctcaggaccg acgtgccact ccgcttcggc accagcggtc
      661 acgtcgtttc ggcctcgagc gacggcctga cccgcacatt ccgggcgacc aacgtccgcg
      721 acttcgtggt cacggcggcg gccgactacc gggtgctcaa gcggcgggtc ggcgacacca
      781 aggtccgtct catcgcccgc cccggcttcc cggccgcccg cgcgctggac gccgcggcca
      841 acgccgtgcg caagctcgaa gccaagctcg gcccataccc gtacgccacg ctccgcgtgg
      901 tccaatccgc cggcgcgtac ggtatggagg ggcccgggat cgcatggatc ccgaccggga
      961 cgcccagcgc aaacctgacc tatctcgtca cccacgagat cgcgcaccag tggttctacg
     1021 gcctcgtggg caacgaccag gcgcgcgagc cgttcgccga cgaggccgcg acggacttcg
     1081 tggcccggcg cgtcctgggg ctccgccgcg gctcgaggtg cgcgagcgac gacctcgacc
     1141 ggtcgatcta ccgctactcc tcgctgtgct actacgaggt cgtctacatc cagggcggca
     1201 acgtgctcga caacgcccgc cagaagatgg gatcgaagcc gttctgggca gcgctccgcg
     1261 gttacgtcgc cgatcaccgc tggggcctca gccacacgcg aaccctgctc gacgcgctgg
     1321 acgcggccac gccgctgaac ctgcgcccga cgtggcgcca tcgattcccg agcctgtact
     1381 gacgatggcg cccatcaccg tccgcctcgc gaccgtcgcc gacggggccg cgtgtgccgc
     1441 gatctacggc ccgttcgtgg ccgggacgtc gatctcgttc gagctgaatc cgccgacggc
     1501 cgaggagatg gccgcgcgca tcgccaaggc gaccgagcgg accccgtggc tcgtggccga
     1561 ggtcgacggg atcgtgcgtg gctacgccta cgcgacgcgc caccgcgatc gggcagcgta
     1621 cgactggacg gtcgagtcgg cggtctatgt cgacggtgcg ttcgcgggac ggggcatcgg
     1681 ccggacggcg atgaccgcgc tgctcgaggt gctgcgcctg cagggcttcc acctcgtggt
     1741 ggccgggatc acgcagccga acccggcctc gacctcgctc cacctggcgc tcggcttcga
     1801 gcggatcggc cagttcgagg caatcggctg gaagcagggc cgctggcacg gggtcgagtg
     1861 gtatgggctg gagctcggcg ggcgggacac cgcaccgacg cctatccgac cgctcgccga
     1921 ggcgatcacg gcatgggacg gccgcgaacg ctaggaccct cccgcgatcc ggcgatccgc
     1981 cgcgagggcc tgggggaacc accgccagcc gggcggcagg acccgtcgcc agaaccccca
     2041 tgtcgtcgtc agccacgcgg agatcacgcg ctcgcgcgcg ccccaggcga acccgtactc
     2101 gtcgcgcagg cctggcggca gcagcccgat cgccggcacg agcagccagt cgtaggctgg
     2161 ggtcggaatc tctcggagca aggcggccgt tggacccgcc caatcgccca gcagcgccgc
     2221 gaccggcccg gcctcgaccg cgggtgcgag cggcggatgg aggatgaccg aggcgaggtc
     2281 gcgggccgtc gccgacgggt gcaccgggcc ggacggcgcc agcatcgagg cgacgtaggc
     2341 gtcgaacgcg tcgacgtcgg caggtatctg cgactccgcg atgccgaaca ggcgggcgac
     2401 cggcatggtc tcggcgtaga acccggcccg gaccgccggc ggaatcgggc ccagccaggc
     2461 ctcgccggtg gccagcgtcg agtcgacgag cgtcgcgtgg acccacagcg cgaggccggg
     2521 atcgcgggcg tcgtaggcgg ccccgaaccg ggcggccgca gcggcatccc gaaccggccc
     2581 gccgatggtt cgatggagcc cgttcagccg acggatctcc gcgtgtgccg ccgtcctcgt
     2641 cccgtacacg atccgcaggt agctgcgcag ggtcccggcg aggcgcgccc acgggtcggc
     2701 ccggaagtcg gagtgctggt ccacgccctc ggccacgagc ggatgcgcga tctggagcag
     2761 gagcgcccgg gggccggcgc cgaggaggag cgccgcctcg cggttgaggc gccaggcctc
     2821 ggacctcggg ccgtagagcc cggcgcgttc gagatcggcg tcgtcgccga agggcggcgt
     2881 cagcggtcgc tcggccgccg cccgaccacc caccaggcca cggggaacac cgccgccgcc
     2941 gcgagcagct tgaccgtgtc gacgacgagg aatgggagca tgccggtctc gatcgcctgg
     3001 cccagggaca tcccggtggt caccgccagc cacggcacgc cgacgaggta gatgaccagc
     3061 gtgccgagcg ccgtggcgcc gatcgcgccc gcgatccgcc ggtcccagcc gagctcggcg
     3121 aggcgcccga cgagggcgcc ggcgaggatg aacccgacga ggtagccgcc ggtcgcaccc
     3181 cagatgacct ggatcccgcc cttgtgctcg gcgaagaacg gcaggccgat gacgccgagc
     3241 gcgacgtaca ggaacgcggc caggccaccc cggcgcaggc cgagcgcgcc gccgacgacc
     3301 aggacgccga aggtctggag cgtgtacggc actggattgc cggggatcgt gaagaacacc
     3361 tgggccgtca ggtagatgag gaccgcgccg gcgatgacga ggacgacgtt gcgcaggcgg
     3421 ggcgagaccc gctcgcccag gccgaccggg acgaggaagt ccgcgatcgt gaggcccttc
     3481 tccgcggcgg gcacccgcgt gatggacggc agcgcgtagg acatcggcct ccctcttggc
     3541 gccgcctggg ggcgagcgcc gcgtcgcgcc gagtctagca acccggggaa gccgggtgga
     3601 ttggacgatc ggtcaggcgc cccggaccgc tgccacgatc gagcgccggc cggcgattcg
     3661 ggccggctgg gcagccgcga gcatcgccag gccgacggcc atcgccagcg acacgccgat
     3721 cgtcacccat gggatctgca ccatcgacgg cagcccgccc gcgaacgcga gcagcagcgt
     3781 gcccacgaca aggcccgtga cgacgccgac ggcacccccg atcacgccca ggattccggc
     3841 ctcgacgagc acgctgcgcc acacctggcg gcggctcatg ccggccgccc gcaggacgcc
     3901 cagctcgcgg ctgcgctccc agctgtccat cgaaagggtg ttggcgatgc cgaagcccgc
     3961 gatgacgacg gccgcgaggg ccagcaggtc gagcagcccg aacacgcgat cgagcgcatc
     4021 accgaccgtc cccgcgaccc gggcaacggg cgccggcgac agcgcgagct gccgggccag
     4081 gtcgtcgaca gccggcatgg ccgtggcagc gcggccggcc gcgtagcgaa cgatgaaggc
     4141 gtcggccccc agcacgccga accgatcgag cgcgtcgccc cagccgacca gcgcggcctc
     4201 gcccgaggcg cccgggaacg atcgctcgac cacgccggcc acggccagct cgacttgccc
     4261 ggccgcggtg gccaccacga tcacggcgcc gacctcgacg cccatgcctc gggcgtcggc
     4321 ggccgggagg atcacagagc cgcccgcgtc gatcgccgcc agcgcggtgg ctcgatcacc
     4381 ggacagtaat gtcagccggc cgtcggcatc caggtcggca ccgcggatgg cgaccgccgg
     4441 catgcgcgtg ccctcgaacg agaggtcgaa cgcggccatc ggggtcacgc gcgccacgcc
     4501 gtcgatcgcc gcaatctcgg actcgtagcc atcggcgccc accggcgcgg gcgcgatagc
     4561 ggtcaggacc tcgtcacccg gcaccacgtc gtccagccac gcggtggcgg cggtccgggc
     4621 gttgacggcg acaacgccga gcgcgaccac catcgccagc cccacgacca gggccccgac
     4681 ggtgatcgtc gtccggccgc ggtcccgggc gatcgccgcg cgagcgagcc gctcctcgag
     4741 gcgcagtacc cgggcgaatg ggaacccggc cacgcggccg agcgggccta gcagcgcggg
     4801 ggtcgccaga acggctgcca gcagcaccac gtagaccacg atggtgcgct gcgacagcgc
     4861 cccggaggac gcgatgccgg gcacgagcac ggccgcgagc acgcccacgg cggccatcac
     4921 gaccagcagc cagccggcgt gcgcacggac gccggccgca gcgtcggacc gcgcccggag
     4981 tgcctcgacg gggctcacgg cgccggcacg acgggccggc tctaccgcgg ccaccagggc
     5041 gacgatcatc cccgcggcga tcccggcgac gatcgcccaa ggggttacga ccgggccgtc
     5101 gagcgtcacg ccgcccgcgg tccgcaacca ggtagcggcc actgccgcga gcagggcccc
     5161 ggccacgatc cccagcgccg agcccagcgc gccgagcaac aggccctggg tgaccacgat
     5221 ccgcccgacc tgggcgcggg t
//
LOCUS       DBNHXO010000573         5600 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate MAG04721
            SRR25158249_k127_4166495, whole genome shotgun sequence.
ACCESSION   DBNHXO010000573 DBNHXO010000000
VERSION     DBNHXO010000573.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414128
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Bacillati; Chloroflexota; Candidatus Limnocylindria;
            Candidatus Limnocylindrales.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:54:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,304
            CDSs (total)                      :: 2,275
            Genes (coding)                    :: 2,265
            CDSs (with protein)               :: 2,265
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4166495"
                     /isolate="MAG04721"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="AC162A_11500"
     CDS             complement(<1..526)
                     /locus_tag="AC162A_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR5011513.1"
                     /translation="MQSSARSRPDPVDVGPWRRRSRDVAYANPWIEVFHDEVDRPDGS
                     PGIYGVVHFRSAAVGVVAVADDGRLLLVGQHRYTLDEYSWEIPEGGVPEGESALEGAR
                     RELREETGFDATDWRQLCRMTVSNSVTDERGVIFVATGLRPGVATPDATEQLAVRWAS
                     LDEVLGEIAAGEIHD"
     gene            695..1705
                     /locus_tag="AC162A_11505"
     CDS             695..1705
                     /locus_tag="AC162A_11505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5011514.1"
                     /translation="MTAGYGERRRRPWPLLFAVLLLGSFLLLAYIVASYAVYDTLSSV
                     SGTCHPADVDNTPEHFTAAGIDEAVTDAYAMPAPQEVEFHSRDPKHPELMLRGWWIPG
                     RMPTSPAVILVHGVGSCRRDPNVLMAAGMLHRHGFGVLLMDLRDHGDSDDEDLRFAGG
                     TEEYLDVLGAWHWLEEQGGVSEERIGIAGMSFGAATTVVAGGEEPRVQAVWEDSSFAD
                     MDQAIRDYLVREGYPTILAPGGELAARIVAGDDLTARGPLMEIPNYTGRPLAIVHGLA
                     DTTLSPHYAEQLRDAAEAAGVDLRDFWLVPGSEHTRAVLEHPDEYESRIVEFFTQTLG
                     TP"
     gene            1728..3830
                     /locus_tag="AC162A_11510"
     CDS             1728..3830
                     /locus_tag="AC162A_11510"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR5011515.1"
                     /translation="MTPHDPRAASQPGHDHDVAWDVRIPARDGLELSANIWRPLPRAD
                     APLARFPAILEMIPYGKDNWRRNWDTAFGEWLAARGFALCRLDVRGTGSSPGVALDEY
                     TEAETLDGFDAVEWLAAQPWCNGNVGMWGISYGGFTSIQVAKLRPPHLRAIAPMYATD
                     DRYRDDVHVRGGCITASEKSQYAVSQLGMNAMPPRPSFRGAGWREEWLARLEQTPPWL
                     LSWVREQLDGPYWRRGSLAPDYEALECAVFQIAGWSDSYVDPAFRIQERCVNAARRTL
                     VGNWVHSFPDDAYPGPNVDWMHEFVRFFDRYLKDVDNGWEDEPALTWFEREWAEPEPF
                     PKEWPGRWRAADTYPVPDSRPMELFLDEDPFSGAYRLQSQGWPGDPDRDTFGGGRMHR
                     MTHRATVGTSGGLSWGAGWPPNGLARDLRPDEQRGLVYTTDPLDEPLSILGVPEAVLW
                     LQATMPVATCVVRLSEVSPDGTSFLVATGALNLTHRRSDSDPTPMPFERRTADQVHIP
                     LRTSGYRFAAGNRIRLTLLTSYWPALWPSPLPGELRVLTGFITPSRVVLPVVPEAAPI
                     EPAISSPAEPASVRDVGSHESDEPVWRIEEDVLAGTVSVTIFDGGASVNEDGSRLYAS
                     ERLVLTASDADPAHAGLASDVVYRWTGDGADVDIRAVGGIESDAESFDVRVALTVRLD
                     DEPFFAREWRERIPRRLV"
     gene            complement(3834..4889)
                     /locus_tag="AC162A_11515"
     CDS             complement(3834..4889)
                     /locus_tag="AC162A_11515"
                     /inference="COORDINATES: protein motif:HMM:NF023178.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HIR5011516.1"
                     /translation="MTDSVLVLTWLVFAHLVADFVLQNDWIAMNKATGGREGSRALAV
                     HGFHVGVCLSPVVLAFGLRGVVYLAVVTITHMIVDRWKVKATRRAEAEAQAQARARVA
                     RTGQAPTSGLGAAWTPWPGILFLGDQVLHLTIAIVGWLVILAGAVLLPQWVDLVNRIL
                     GTWDRATVNAVILTGLVIVSLFIVNTRAAYYFVLSLVSPRELTPDPDAPAQPEPPRPT
                     PAGYTVRVGPLVATVEPTLAGAGPEPDDDDVNAAEQASPTASPVPGPVAAVPTGAPAR
                     IGATIGALERLLIVAFMLTGAEAAVGFVIAAKTIARFKQLDDRGFAEYYLLGTLASVA
                     VAISTSLVAIAALSSLS"
     gene            complement(4886..5578)
                     /locus_tag="AC162A_11520"
     CDS             complement(4886..5578)
                     /locus_tag="AC162A_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5011517.1"
                     /translation="MTARSAGTVLFGDVVDSRRDPGSSDWLRALCAELEGAYPRADRL
                     AAFAFTQGDEVQGLLAPGVDPFRAVLHAALQPDARSLRWAIVAGDIDPGTGPATERNG
                     DAFLVAREAIARAKAHREGLVAISGDPVADGLLADTGPLLAALLADLTSRQREVARLM
                     IIDEMRQSQAAEHLGVSRATISVVADRARVRHLQGLGRTLASIFDQGVRRTMVEPGAP
                     TTLTTGTPVSPA"
BASE COUNT          802 a         1971 c         2036 g          791 t
ORIGIN      
        1 ggtcgtggat ctcgcccgct gcgatctcgc ccagcacctc gtccaggctc gcccagcgca
       61 cggcgagctg ctcggtcgcg tccggtgtcg cgacgccggg gcggaggccg gtcgcgacga
      121 agatcacgcc ccgttcgtcg gtgaccgagt tggacacggt catccggcac agctggcgcc
      181 agtccgtcgc gtcgaagccg gtctcctcgc gcagctcgcg gcgcgcaccc tcgagcgccg
      241 actccccctc cggcacgccg ccctctggga tctcccagct gtactcgtcg agcgtatagc
      301 ggtgctggcc gacgagcagg agccgcccgt cgtcggccac ggccacgacg ccgaccgccg
      361 ccgagcggaa gtgcaccacg ccatagatgc cggggctccc gtcggggcgg tcgacctcgt
      421 cgtggaagac ttcgatccag gggttcgcgt aggcgacgtc acgcgaccgg cggcgccacg
      481 gcccgacgtc gaccggatcg ggcctggatc gggcgctcga ctgcacgtcg caacggtagc
      541 ggtcgattcg cgtcgatgcc tgtggataac acgtgatgga tgtggatgac cgccgcacgg
      601 cgactcgcct gaaggcggcc cggtgcggcg gggcaacgcg tatcgtgtgc cgatcggtgg
      661 tcgtcgccgg gagggtgggc tgtatggcat gcggatgacg gctggctacg gtgagcgacg
      721 gcggcgacca tggccgctcc tgttcgccgt gctgctgctc gggtcgttcc tgctcctcgc
      781 ttacatcgtg gcgagctatg ccgtctacga caccctgtcg tccgtctccg gcacctgcca
      841 tcccgccgac gtcgacaaca cgcccgagca cttcaccgcg gccgggatcg acgaggcggt
      901 caccgacgcg tatgcaatgc cggcgccgca ggaggtggag ttccactccc gcgacccgaa
      961 gcatcccgag ctgatgctgc gcggctggtg gatcccgggt cgcatgccga ccagtcctgc
     1021 ggtgatcctg gtccacggcg tgggcagctg ccggcgagac ccgaacgtgc tgatggccgc
     1081 cggaatgctg catcgtcacg gcttcggcgt gctgctgatg gaccttcgcg accacggcga
     1141 cagcgacgac gaggacctgc ggttcgccgg cgggaccgag gagtatctcg acgtcctcgg
     1201 cgcctggcac tggctcgagg agcagggcgg cgtgtccgag gagcggatcg ggatcgccgg
     1261 catgtcgttt ggggctgcga cgacggtggt cgccggaggc gaggagccgc gcgtccaggc
     1321 tgtctgggag gactcgtcgt tcgcggacat ggaccaggca atccgcgact acctcgtccg
     1381 cgagggctat ccgacgatcc tcgccccggg cggcgagctt gcggcgcgaa tcgtcgccgg
     1441 tgacgacctg acggcgaggg ggccgctcat ggagatcccc aactacacgg ggcggccgtt
     1501 ggcgatcgtc cacggattgg ccgacacgac gctgagcccc cactacgcgg agcagctgcg
     1561 cgacgccgcg gaagcggcgg gcgtggactt gcgggacttc tggctggtcc ccggctccga
     1621 acacacacgg gccgtcctgg agcatcccga cgagtacgag tcccgcatcg tcgagttctt
     1681 cacccagacg ctcgggacgc catagcggtc cgcgtatcct cgggcccgtg acccctcacg
     1741 atccgcgcgc cgcgagccag cctggccacg atcacgacgt cgcgtgggac gtgcggatcc
     1801 ccgcgcggga tggtctcgag ctgtcggcca acatctggcg cccactcccc cgggccgatg
     1861 cgccactcgc gcgcttcccc gcgatcctcg agatgatccc gtacggcaag gacaactggc
     1921 gccgaaactg ggacacggcc ttcggggagt ggctcgcggc acgcggcttc gccctctgcc
     1981 gcctcgacgt ccggggcacc gggagctcgc cgggcgtcgc gctcgacgag tacacggaag
     2041 ccgagacgct cgacgggttc gacgccgtgg agtggctcgc ggcccaaccc tggtgcaacg
     2101 ggaacgtcgg catgtgggga atcagctacg ggggcttcac gtcgatccag gtcgcgaagc
     2161 tgcgcccgcc gcacctgcga gcgatcgccc cgatgtacgc caccgacgac cgctaccggg
     2221 acgatgtcca tgtccgcggc ggctgcatca cggcgagcga gaagagccaa tacgccgtca
     2281 gccagctggg catgaacgcg atgccgccac ggccgtcgtt ccgcggcgcg ggctggcggg
     2341 aggagtggct ggcgcgcctc gagcagacgc cgccgtggct cctgtcctgg gtccgcgagc
     2401 agctcgatgg cccgtactgg cggcgggggt cgctggcgcc cgactacgag gcactcgaat
     2461 gcgcggtatt ccagatcgcc ggctggagcg actcgtacgt ggatcctgcg ttccgcatcc
     2521 aggagcgatg cgtcaacgcc gcccgccgga ccctggtcgg caactgggtc cactcgttcc
     2581 cggacgatgc ctacccgggc ccgaacgtcg actggatgca cgagttcgtg cgcttcttcg
     2641 accgctacct caaggacgtc gacaacggct gggaggacga gccggcactc acctggttcg
     2701 agcgcgagtg ggcggagccc gagccgttcc ccaaggaatg gcccgggcga tggcgggctg
     2761 ccgatacgta tcccgtgccc gactcccggc ccatggagct gttcctcgac gaggacccct
     2821 tcagcggtgc gtaccgcctg caatcgcagg gttggcctgg cgatccggac cgtgacacgt
     2881 tcggtggggg ccgcatgcac cggatgaccc accgcgcgac ggtcggcacg agcggcgggc
     2941 tgtcgtgggg ggccggctgg cccccgaacg gactggcgcg tgacctccgc cccgacgagc
     3001 agcgcgggct cgtgtacacg accgacccgc tggacgagcc gctgtccatc ctgggcgtgc
     3061 ccgaggcggt cctctggctg caggcgacga tgcctgtcgc gacctgcgtc gtccgcctct
     3121 cggaggtgtc gcccgacggg acgtcattcc tcgtcgccac cggcgctctg aacctcaccc
     3181 accgccgctc cgacagcgac cccaccccga tgcccttcga gcggcggacc gccgaccagg
     3241 tgcacatccc gctgcggacg agcggctacc ggttcgcggc agggaaccgg atccgcctga
     3301 ccctgctcac ctcgtactgg cccgcgctgt ggccatcgcc gctgcccggc gagctgcggg
     3361 tcctgaccgg cttcatcacc ccgtcgcggg tcgtgctgcc ggtcgtgccg gaggccgcgc
     3421 cgattgagcc cgcaatctca tcgcccgcgg aaccggcatc ggtccgcgac gtcgggagcc
     3481 acgagtcgga cgagcccgtg tggcggatcg aggaggacgt ccttgccggg acggtttcgg
     3541 tgacgatctt cgacggcggg gcgagcgtca acgaggacgg ctcgcgcctg tatgcctcgg
     3601 agcgcctggt cctgaccgcc tcggacgccg acccggccca cgccggcctc gcgagcgacg
     3661 tcgtctaccg ctggacgggc gacggcgccg acgtcgacat ccgggccgtc ggcgggatcg
     3721 agtcggatgc cgagtcgttc gacgtgcggg tcgcgctcac cgtccgcctc gacgacgagc
     3781 cgttcttcgc gcgcgagtgg cgggagcgga tcccgcgccg gctggtctga ccgtcaggac
     3841 agcgagctga gggcggcgat cgcgacgagc gacgtgctga tcgccacggc gacgctcgcg
     3901 agcgttccca gcaggtagta ctcggcgaag ccgcgatcgt cgagctgctt gaaccgggcg
     3961 atggtcttgg ccgcgatgac gaacccgacg gccgcctctg cgcccgtgag catgaacgcg
     4021 acgatcagca ggcgctcgag cgcgccgatc gtcgcgccga tcctggccgg ggcgccggtc
     4081 ggtaccgcgg ccacgggtcc gggcaccggg ctcgccgtcg ggctggcctg ctcggcggcg
     4141 ttgacgtcat cgtcgtccgg ctcgggacct gcgccggcaa gcgtcggctc gacggtcgcg
     4201 acgagcggcc cgacgcgcac ggtgtagcca gcgggggtcg ggcgaggcgg ctcgggctgg
     4261 gccggcgcgt cggggtcggg cgtgagctcg cgcggcgaca cgagcgagag gacgaagtag
     4321 taggcggccc gcgtgttgac gatgaacagc gacacgatga cgaggccggt caggatcacg
     4381 gcattgacgg tagcccgatc ccaggtgccc aggatgcggt tgaccaggtc gacccactgc
     4441 ggcagcagga ccgcgcccgc gaggatcaca agccagccca cgatcgcgat cgtgaggtgg
     4501 agcacctggt cgcccaggaa caggatcccg ggccacgggg tccacgccgc gccaagcccc
     4561 gaggtcggtg cctgtcccgt ccgggcgacg cgagcccgtg cctgcgcctg ggcctcggct
     4621 tccgcccgcc gcgtcgcctt gaccttccag cggtcgacga tcatgtgggt gatcgtgacg
     4681 accgcgaggt agacgacgcc gcgcagcccg aatgccagca cgaccggcga caggcacacg
     4741 ccgacgtgga acccgtggac ggcgagggca cgcgacccct cccggccacc cgtcgccttg
     4801 ttcatcgcga tccagtcgtt ttggaggacg aagtcggcga ccaggtgggc gaacaccagc
     4861 caggtcagga cgaggacgga atcggtcatg ccgggctcac aggcgttccg gtcgtgagcg
     4921 tcgtcggcgc gcccggctcg accatcgtcc gccggacgcc ctggtcaaag atcgaggcga
     4981 gggtccggcc gaggccctgc aggtgacgga cacgggcgcg atcggcgacg acggagatcg
     5041 ttgcgcggct gacgccgagg tgttccgccg cctgggactg gcgcatctcg tcgatgatca
     5101 tcaggcgggc gacctctcgc tggcgcgacg tgaggtcggc gaggagcgcc gccaggagcg
     5161 ggccggtgtc ggcgagcagg ccatccgcca cggggtcgcc ggagatggca accagcccct
     5221 cgcggtgggc cttcgccctg gcgatcgcct cccgggcgac caggaacgcg tcgccgttgc
     5281 gctcggtggc ggggccagtg ccgggatcga tgtcgccggc gacgatcgcc caacgcaggc
     5341 tgcgggcgtc gggctggagc gccgcgtgga ggacggcccg gaacgggtcg acgcccggcg
     5401 ccagcaggcc ctggacctcg tcgccctgtg taaacgcgaa cgccgcgagg cggtcggcgc
     5461 gcgggtacgc gccctcgagc tcggcgcaca gcgcgcggag ccagtcgctg gagcccgggt
     5521 cccgccgcga gtcgacgacg tcgccgaaca ggacggtccc ggcagagcgg gcagtcatcc
     5581 ggccgagcct actcgatggc
//
